The susceptibility of three strains of Chinese minipigs to diet-induced type 2 diabetes mellitus.
Researchers have extensively used animal models to study diabetes mellitus. In this study, the authors determined the susceptibility of three strains of Chinese minipigs to diet-induced type 2 diabetes mellitus. For 8 months, the researchers fed Nongda control minipigs (n=4) a normal diet and fed Bama, Wuzhishan and Nongda minipigs (n=6 per group) a high-sucrose, high-fat diet. They measured the minipigs' body weights, fasting serum glucose concentrations and insulin concentrations each month. Every 2 months, they measured serum triglyceride, cholesterol, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels and carried out intravenous glucose tolerance tests (IVGTTs). The Bama and Wuzhishan minipigs were relatively susceptible to diabetes induced by the high-sucrose, high-fat diet, though susceptibility differed among individual animals in the same strain. On the other hand, Nongda minipigs were relatively resistant to diet-induced diabetes. These results provide a foundation for diabetes-related genetic analyses in minipigs with high and low susceptibility to diet-induced type 2 diabetes.
Chen
Hua
H
Animal Experiment Center of General Hospital of PLA, Beijing, China. chenhua@301hospital.com.cn
Liu
Ya-qian
YQ
Li
Chun-hai
CH
Guo
Xiong-ming
XM
Huang
Li-jie
LJ
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D007328:Insulin; D014280:Triglycerides; D002784:Cholesterol