Novel hepatitis B virus subgenotype in the southern Yunnan Province of China.
Hepatitis B virus (HBV) infection is highly prevalent in China. To identify the genotypes of HBV in the southern Yunnan Province of China, full-length HBV genomes were extracted from 1 Dai and 4 Hani HBV carriers and linked with the pMD T-18 vector. For each patient, 3-10 clones were sequenced directly and a consensus sequence was created. Genotypic and serotypic analysis revealed 4 HBV/B (2 B2 with adw2 and 2 new subgenotypes with ayw1) and 1 HBV/C (C1 with adrq+) genotypes. The divergences of the entire genome sequences of the new subgenotype were 0-0.9% and 2.99-6.48% between other known HBV/B. Divergences in other coding regions revealed that it was more similar to B3 and B4 in the precore/core gene (2.02 and 2.09%, respectively), and similar to B3 and B5 in the preS1/S2/S gene (2.24 and 2.78%, respectively). Phylogenetic trees using the precore/core and X genes both revealed a new clad separating from the major trunk of genotype B with a 99% bootstrap value. These results show that the 2 consensus isolates are a mosaic of B3-B5, which we designated to subgenotype B6. Considering the geographical distances, the relationship between B6 and other HBV/B subgenotypes (B3-B5) and HBV evolution needs to be further studied.
Shen
Tao
T
Institute of Basic Medicine of the First People's Hospital of Yunnan Province, Kunming 650032, Yunnan Province, PR China.
Gao
Jian-mei
JM
Zou
Yun-Lian
YL
Dong
Hong
H
Yan
Xin-Min
XM
D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D006509:Hepatitis B / Q000453:epidemiology* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004279:DNA, Viral