An artificial extracellular matrix created by hepatocyte growth factor fused to IgG-Fc.
The design of artificial extracellular matrices (ECM) has attracted much attention in tissue engineering and regenerative medicine as well as in molecular biology research. A recombinant hepatocyte growth factor (HGF), fused to an immunoglobulin G (IgG) Fc region (abbreviated as AeHGF-Fc) was constructed and confirmed by Western blot assay. Almost similar amounts of HepG2 cells adhered to AeHGF-Fc-coated surface compared to collagen-coated one with large morphological changes. Immobilized AeHGF-Fc continuously activated Akt in HepG2 cells whereas Akt activation induced by soluble HGF rapidly decreased with time, indicating that immobilized AeHGF-Fc follows different signal transduction pathways compared to soluble HGF.
Department of Biomolecular Engineering, Tokyo Institute of Technology, Japan.
Koji
K
Azuma
Masato
M
Nagaoka
Chong-Su
CS
Cho
Toshihiro
T
Akaike
D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism*; D056945:Hep G2 Cells; D017228:Hepatocyte Growth Factor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008422:Materials Testing; D011961:Receptors, Fc / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods
D007074:Immunoglobulin G; D011961:Receptors, Fc; D011993:Recombinant Fusion Proteins; D017228:Hepatocyte Growth Factor