Kinetics of {alpha}2-macroglobulin and {alpha}1-acid glycoprotein in rats subjected to repeated acute inflammatory stimulation.
The kinetics of alpha(2)-macroglobulin (alpha2M) and alpha(1)-acid glycoprotein (AAG) in rats repeatedly stimulated with intramuscular injections of turpentine oil at doses 0.05 and 0.4 mL/rat were investigated. Mean serum levels of alpha2M peaked at 48 h after the first turpentine oil injection, reaching 1.74 and 2.36 mg/mL at 0.05 and 0.4 mL/rat, respectively. AAG peaks were also observed at 48 h after injection, and the mean values were 2.02 and 2.53 mg/mL, respectively. These peak values of alpha2M and AAG differed significantly between the 0.05 and 0.4 mL/rat injection groups. Mean serum levels of interleukin-6 (IL-6) at 0.05 mL/rat were 52.61 pg/mL at 12 h, 48.86 pg/mL at 36 h and 81.93 pg/mL at 84 h after the first injection. Mean IL-6 serum levels at 0.4 mL/rat were 215.24 pg/mL at 12 h, 56.33 pg/mL at 36 h and 39.25 pg/mL at 84 h after the first injection. Mean serum levels of cytokine-induced chemoattractant-1 (CINC-1) at a dose of 0.05 mL/rat were 5.70 ng/mL at 12 h, 5.58 ng/mL at 36 h and 4.58 ng/mL at 84 h after the first injection. Mean serum levels of CINC-1 after injection at 0.4 mL/rat were 11.57 ng/mL at 12 h, 4.68 ng/mL at 36 h and 4.42 ng/mL at 84 h. Serum levels of IL-6 differed significantly at 12, 24, 72 and 84 h, while those of CINC-1 differed significantly at 12, 24, 48 and 96 h between the 0.05 and 0.4 mL/rat injection groups. Differences in peak serum levels in the 0.05 and 0.4 mL/rat groups were attributed to differences in the production of IL-6 and CINC-1, which are thought to contribute to alpha2M and AAG production.
Azabu University, Fuchinobe, Sagamihara, Kanagawa, Japan.
Toshio
T
Honjo
Takashi
T
Kuribayashi
Mariko
M
Matsumoto
Shunsuke
S
Yamazaki
Shizuo
S
Yamamoto
D000210:Acute-Phase Reaction / Q000097:blood*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D054360:Chemokine CXCL1 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism*; D005779:Immunodiffusion; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D008297:Male; D009961:Orosomucoid; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014425:Turpentine / Q000633:toxicity; D000511:alpha-Macroglobulins / Q000378:metabolism*
D001798:Blood Proteins; D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D006023:Glycoproteins; D015850:Interleukin-6; C506108:Orm1 protein, rat; D009961:Orosomucoid; D000511:alpha-Macroglobulins; D014425:Turpentine