Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01.
Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).
Kim
Do Young
DY
Industrial Bio-materials Research Center, KRIBB, Daejeon, Republic of Korea.
Park
Doo-Sang
DS
Kwon
Soon Bum
SB
Chung
Moon Gyu
MG
Bae
Kyung Sook
KS
Park
Ho-Yong
HY
Rhee
Young Ha
YH
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D019117:Burkholderia / Q000378:metabolism / Q000502:physiology*; D001517:Coleoptera / Q000378:metabolism* / Q000502:physiology*; D005942:Gluconates / Q000378:metabolism; D007982:Levulinic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011091:Polyesters / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D005942:Gluconates; D007982:Levulinic Acids; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C030691:gluconic acid; C032246:levulinic acid