Sensing of peptide hormones with dynamic combinatorial libraries of metal-dye complexes: the advantage of time-resolved measurements.
A dynamic combinatorial library of metal-dye complexes was obtained by reacting aqueous solutions of the dyes Methyl Calcein Blue, Arsenazo I, and Xylenol Orange with CuCl(2) and NiCl(2). The mixture gave a characteristic UV-Vis response upon addition of the peptide hormones angiotensin I and angiotensin II. This allowed distinguishing pure samples of peptide hormones from mixtures. The discriminatory power of the sensor was enhanced when the several UV/Vis measurements were performed during the equilibration process of the library.
Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Friederike
F
Zaubitzer
Thomas
T
Riis-Johannessen
Kay
K
Severin
D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D004396:Coloring Agents / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D016002:Discriminant Analysis; D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D036361:Peptide Hormones / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D017186:Titrimetry
D004396:Coloring Agents; D008670:Metals; D036361:Peptide Hormones; D000804:Angiotensin II; D000803:Angiotensin I