Three-stage hydrolysis to enhance enzymatic saccharification of steam-exploded corn stover.
The objective of the present research was to explore new approach to reduce the hydrolysis time and to enhance the productivity of enzymatic saccharification. One-stage hydrolysis of steam-exploded corn stover required 72 h to reach a yield of 62.8%, while multi-stage hydrolysis could reduce the time to 24 h. A concept of three-stage hydrolysis was therefore proposed in which cellulosic substrate was hydrolyzed for 6, 6, and 12 h, respectively. High hydrolysis yields, 70.2% with enzyme recycling and 76.1% with the supplement of fresh enzyme to eliminate enzyme recovery procedure, were obtained in 24 h. Analysis indicated that short-time hydrolysis and the removal of end products at each stage improved cellulase activities and benefited the adsorption of cellulase enzyme to the solid substrate. When steam-exploded corn stover was used as the substrate for cellulase synthesis, a hydrolysis yield of 88.6% was achieved in 24 h.
Yang
Jing
J
Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Zhang
Xiaoping
X
Yong
Qiang
Q
Yu
Shiyuan
S
D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013227:Steam; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
D002241:Carbohydrates; D004798:Enzymes; D013227:Steam; D002482:Cellulose; D002480:Cellulase