On-chip electrical impedance tomography for imaging biological cells.
Electrical impedance tomography is an imaging technology that spatially characterizes the electrical properties of an object. We present a miniaturized electrical impedance tomography system that can image the electrical conductivity distribution within a two-dimensional cell culture. A chip containing a circular 16-electrode array was fabricated using printed circuit board developing technology and used to inject current and to measure spatial voltage across the object. The signal stimulation and voltage data acquisition were performed using an impedance analyzer, operating in four-electrode mode. An open source software, EIDORS was used for image reconstruction. Finite element modelling was used to simulate the image reconstruction process by imaging two ellipsoidal phantoms in the circular 16-electrode array. The effect of the regularization parameter in the reconstruction algorithm and the influence from noise on the fidelity of the images has been numerically analyzed. Experimentally, we show reconstructed images of a multi-nuclear single cellular organism, Physarum Polycephalum, demonstrating the first step towards impedance imaging of single cells in culture. Our system provides a non-invasive lab-on-a-chip technology for spatially mapping the electrical properties of single cells, which would be significant and useful for diagnostic and clinical applications.
Sun
Tao
T
School of Electronics and Computer Science, University of Southampton, United Kingdom. ts5@ecs.soton.ac.uk
Tsuda
Soichiro
S
Zauner
Klaus-Peter
KP
Morgan
Hywel
H
D015374:Biosensing Techniques / Q000295:instrumentation*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D016839:Physarum polycephalum / Q000166:cytology / Q000502:physiology*; D010992:Plethysmography, Impedance / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014054:Tomography / Q000295:instrumentation*