Vitamin A supplementation induces oxidative stress and decreases the immunocontent of catalase and superoxide dismutase in rat lungs.
Lungs require an adequate supply of vitamin A for normal embryonic development, postnatal maturation, and maintenance and repair during adult life. However, recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer, although the mechanisms underlying this effect are still unknown. Here, the authors studied the effect of vitamin A supplementation on oxidative stress parameters in lungs of Wistar rats. Vitamin A supplementation either at therapeutic (1000 and 2500 IU/kg) or excessive (4500 and 9000 IU/kg) doses for 28 days induced lipid peroxidation, protein carbonylation, and oxidation of protein thiol groups, as well as change in catalase (EC 1.11.1.6; CAT) and superoxide dismutase (EC 1.15.1.1, SOD) activities and immunocontents. These results altogether suggest that vitamin A supplementation causes significant changes in redox balance the free radical status in lungs, which are frequently associated to severe lung dysfunction.
Centro de Estudos em Estresse Oxidativo, Universidade Federal do Rio Grande do Sul, Departamento de Bioquímica, Porto Alegre, Brazil.
Matheus A B
MA
Pasquali
Daniel P
DP
Gelain
Marcos R
MR
Oliveira
Guilherme A
GA
Behr
Leonardo L
LL
Motta
Ricardo F
RF
Rocha
Fábio
F
Klamt
José C F
JC
Moreira
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D019587:Dietary Supplements / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D015151:Immunoblotting; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology* / Q000633:toxicity
D013438:Sulfhydryl Compounds; D014801:Vitamin A; D002374:Catalase; D013482:Superoxide Dismutase