Incorporation of the chlorophyll d-binding light-harvesting protein from Acaryochloris marina and its localization within the photosynthetic apparatus of Synechocystis sp. PCC6803.
The gene encoding a chlorophyll d-binding light-harvesting protein, pcbA from Acaryochloris marina (now called as accessory Chlorophyll Binding Protein CBPII) marked with a His-tag was transformed into the genome of Synechocystis PCC6803. Protein gel electrophoresis and western blotting confirmed that this foreign chlorophyll d-binding protein CBPII was expressed and integrated into the thylakoid membrane and bound with chlorophyll a, the only type of chlorophyll present in Synechocystis PCC 6803. Native electrophoresis suggested that CBPII interacts with photosystem II of Synechocystis PCC 6803. Surprisingly, spectral analyses showed that the phycobiliproteins were suppressed in the transformed Synechocystis pcbA(+), with a lower ratio of phycobilins to chlorophyll a. These results suggest that there are competitive interactions between the external antenna system of phycobiliproteins and the integral antenna system of chlorophyll-bound protein complexes.
Yang
Daping
D
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, PR China.
Qing
Yang
Y
Min
Chen
C
D015153:Blotting, Western; D002734:Chlorophyll / Q000378:metabolism*; D015151:Immunoblotting; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology*; D052980:Phycobilins / Q000378:metabolism; D052979:Phycobiliproteins / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism
D045342:Light-Harvesting Protein Complexes; D052980:Phycobilins; D052979:Phycobiliproteins; D002734:Chlorophyll; C107509:chlorophyll d