Developing VUV spectroscopy for protein folding and material luminescence on beamline 4B8 at the Beijing Synchrotron Radiation Facility.
The new 4B8 beamline provides UV-VUV light in the wavelength range from 360 to 120 nm. It uniquely enables two kinds of spectroscopy measurements: synchrotron radiation circular dichroism spectroscopy and VUV excited fluorescence spectroscopy. The former is mainly used in protein secondary structure studies, and the latter in VUV excited luminescent materials research. Remote access to fluorescence measurement has been realised and users can collect data online. Besides steady-state measurements, fluorescence lifetime measurements have been established using the time domain method, while a laser-induced temperature jump is under development for protein folding dynamics using circular dichroism as a probe.
Tao
Ye
Y
Beijing Synchrotron Radiation Facility (BSRF), Institute of High Energy Physics, Chinese Academy of Sciences, People's Republic of China. taoy@ihep.ac.cn
Huang
Yan
Y
Gao
Zhenghua
Z
Zhuang
Hao
H
Zhou
Aiyu
A
Tan
Yinglei
Y
Li
Daowu
D
Sun
Shuaishuai
S
D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry; D017510:Protein Folding; D012709:Serum Albumin / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D017356:Synchrotrons / Q000295:instrumentation*
D009211:Myoglobin; D012709:Serum Albumin