The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex.
The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in severe defects in biorientation and organization of the spindle midzone and midbody. Our results indicate that a major function of the Nup107 complex in mitosis is to ensure the proper localization of the CPC at the centromere.
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH93JR, United Kingdom. m.platani@ed.ac.uk
Melpomeni
M
Platani
Rachel
R
Santarella-Mellwig
Markus
M
Posch
Rudolf
R
Walczak
Jason R
JR
Swedlow
Iain W
IW
Mattaj
D000705:Anaphase / Q000187:drug effects; D064107:Aurora Kinase B; D064127:Aurora Kinases; D020090:Chromosome Segregation / Q000187:drug effects; D002877:Chromosomes, Human / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D048749:Cytokinesis / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D018386:Kinetochores / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013379:Substrate Specificity / Q000187:drug effects; D013871:Thiones / Q000378:metabolism
C091489:KIF2C protein, human; C512772:NUP107 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011743:Pyrimidines; D013871:Thiones; C400223:monastrol; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D016547:Kinesins; D017239:Paclitaxel; D015739:Nocodazole