[Clinical evaluation of the application of gene chip for identifying pathogens in blood cultures].
OBJECTIVE
To explore the feasibility of using gene chip method to identify pathogens in blood cultures.


METHODS
Clinical blood samples were obtained and cultured using an automated blood culture system. A gene chip diagnostic kit was used to detect the pathogenic bacteria in these blood cultures following the procedures of target gene extraction and amplification, hybridization and result analysis. The conventional method was also used to isolate and identify the bacteria from the clinical blood cultures, and the results of the two methods were compared.


RESULTS
In the 86 clinical blood samples, 74 were positive and 12 negative according to the conventional method, while 48 were positive and 38 negative as found by the gene chip method, showing significant differences in the results (P<0.05). The two methods only had a concordance rate of 69.77%.


CONCLUSIONS
The gene chip diagnostic kit has low concordance rate with the conventional method for detecting pathogens in clinical blood cultures and awaits further improvement.
Li
Lin-hai
LH
Department of Clinical Laboratory, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. mature303@126.com
Cheng
Ying
Y
Chen
Li-dan
LD
Huang
Xiao-yan
XY
Shi
Yu-ling
YL
He
Jie-jing
JJ
Wang
Lu-xia
LX
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S