Isolation and characterization of murine multipotent lung stem cells.
The capacity of the lung to repair itself after injury is well known, but the cell types involved in lung regeneration remain undefined. The aim of this study was to isolate and characterize resident progenitor/stem cells from adult mouse lung. We report the isolation and characterization of resident stem cells that have a Sca1+/CD45(-)/CD31(-) phenotype. Their immunophenotype and differentiative potentiality were distinct from that of other previously described lung stem cells. These cells underwent extensive self-renewal in culture and could differentiate into endothelial and lung epithelial (alveolar type I, II, and Clara) cells in vitro. They have exhibited some mesenchymal but no neural differentiation ability. Transfer of these cells into mouse models of lung injury significantly improved survival and minimized lung destruction. These cells may provide useful tools for the study of lung stem cells and the assessment of new therapeutic approaches for lung diseases.
Department of Advanced Preventive Medicine for Infectious Disease, Tohoku University School of Medicine, Sendai, Japan. hegabb@ucla.edu
Ahmed E
AE
Hegab
Hiroshi
H
Kubo
Naoya
N
Fujino
Takaya
T
Suzuki
Mei
M
He
Hidemasa
H
Kato
Mutsuo
M
Yamaya
D056809:Alveolar Epithelial Cells / Q000166:cytology; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D005434:Flow Cytometry; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D055370:Lung Injury / Q000473:pathology / Q000628:therapy; D008565:Membrane Proteins / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D009474:Neurons / Q000166:cytology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens