Analysis of Foxp3, CD25, and CD127 expressed on regulatory T cells in Thai subjects.
Foxp3+ natural regulatory T cells (nTreg) play a distinct role in maintaining self tolerance at the periphery. CD25hi and CD127lo were proposed for the identification and purification of nTreg but they have not been confirmed in non-Caucasian populations. This study examined the sensitivity and purity of Foxp3 nTreg identified by CD25 and CD127 in the peripheral blood of Thai subjects (13 males, 15 females with age range of 20-42 years old). The proportions of nTreg/CD4+ as identified by the different markers were as follows: Foxp3+, 18.3 +/- 6.4%; CD25hi 6.4 +/- 3.2%; and CD127lo, 54.3 +/- 14.2%. Sensitivity tests showed the following results: CD25hi, 23.1%; CD127lo, 40.6%; CD25hiCD127lo, 7.4%. Purity tests concluded as follows: CD25hi, 63.6%; CD25int, 24.9%; CD2510, 8.7%, CD127lo, 26.5%; CD127hi, 14.9%, and CD25hiCD127lo, 52.0%. In conclusion, the proportions of nTreg in Thai subjects are similar to Caucasian populations. CD25hi is superior to CD127lo for separating Foxp3+ nTreg. Combining CD25hi and CD127lo does not improve the nTreg purity.
Thongpan
Masatha
M
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Kitiyakara
Chagriya
C
Louischaroen
Yada
Y
Keesukphan
Prasit
P
Chaiyaroj
Sansanee C
SC
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002452:Cell Count; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology / Q000378:metabolism*; D053699:Interleukin-7 Receptor alpha Subunit / Q000276:immunology / Q000378:metabolism*; D008297:Male; D012680:Sensitivity and Specificity; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013785:Thailand; D044465:White People
D000954:Antigens, Surface; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit