[In vitro trials with single and co-cultures of osteoblasts and endothelial cells : evaluation of new biomaterials for bone reconstruction and regeneration].
Many different types of bone substitute biomaterials are being developed for different applications in the body. The current dogma is that if osteoblasts and endothelial cells grow and exhibit normal cell functions on these materials in vitro as single cultures or in co-cultures, then the biomaterials are suitable for implantation for bone reconstruction and regeneration. Generally, only in vivo animal studies will prove whether this is the case. However, in vitro studies offer a good pre-screening and selection basis to evaluate the biocompatibility of novel biomaterials prior to animal studies. Multicell type co-culture systems hold a great promise for the future.
Unger
R E
RE
REPAIR Lab, Institut für Pathologie, Johannes-Gutenberg-Universität, Langenbeckstrasse 1, 55133, Mainz, Deutschland. runger@uni-mainz.de
Halstenberg
S
S
Günther
H
H
Sartoris
A
A
Brochhausen
C
C
Kirkpatrick
C J
CJ
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry* / Q000627:therapeutic use; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing / Q000379:methods; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes