Aptamers as a model for functional evaluation of LNA and 2'-amino LNA.
The affinity change upon incorporation of LNA and 2'-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2'-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.
Hernandez
Frank J
FJ
Biochemistry and Molecular Biology Department, Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense M 5230, Denmark.
Kalra
Neerja
N
Wengel
Jesper
J
Vester
Birte
B
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry; D001665:Binding Sites; D007700:Kinetics; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013997:Time Factors
D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; C477371:locked nucleic acid; D001360:Avidin