Electrical stimulation promotes nerve cell differentiation on polypyrrole/poly (2-methoxy-5 aniline sulfonic acid) composites.
The purpose of this work was to investigate for the first time the potential biomedical applications of novel polypyrrole (PPy) composites incorporating a large polyelectrolyte dopant, poly (2-methoxy-5 aniline sulfonic acid) (PMAS). The physical and electrochemical properties were characterized. The PPy/PMAS composites were found to be smooth and hydrophilic and have low electrical impedance. We demonstrate that PPy/PMAS supports nerve cell (PC12) differentiation, and that clinically relevant 250 Hz biphasic current pulses delivered via PPy/PMAS films significantly promote nerve cell differentiation in the presence of nerve growth factor (NGF). The capacity of PPy/PMAS composites to support and enhance nerve cell differentiation via electrical stimulation renders them valuable for medical implants for neurological applications.
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia.
Xiao
X
Liu
Kerry J
KJ
Gilmore
Simon E
SE
Moulton
Gordon G
GG
Wallace
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D017097:Electric Impedance; D004558:Electric Stimulation; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D051381:Rats; D013451:Sulfonic Acids / Q000737:chemistry*
D000814:Aniline Compounds; D001672:Biocompatible Materials; D011108:Polymers; D011758:Pyrroles; D013451:Sulfonic Acids; C525236:poly(2-methoxyaniline-5-sulfonic acid); C067635:polypyrrole; D020932:Nerve Growth Factor