Preparation of N,O-carboxymethyl chitosan nanoparticles as an insulin carrier.
The aim of this research was to develop pH-sensitive insulin-loaded NOCC (N,O-carboxymethyl chitosan) nanoparticles for the controlled release of insulin via the oral route. Thus, in this study, insulin-loaded NOCC nanoparticles were prepared by ionic gelation of NOCC with TPP (tripolyphosphate). NOCC nanoparticles were formed at conditions of 2 mg/ml of NOCC and 1 mg/ml of TPP. It was found that the encapsulation efficiency and process yield decreased with increasing NOCC to TPP weight ratio. Furthermore, the cumulative release of insulin from insulin-loaded NOCC nanoparticles decreased with decreasing NOCC-to-TPP weight ratio, but it increased with decreasing the initial concentration of insulin. The higher the pH of the phosphate buffered saline, the greater the amount of cumulative release of insulin-loaded NOCC nanoparticles, and thus they could protect insulin from acid.
Lin
Chuen-Chang
CC
Department of Chemical & Materials Engineering, National Yunlin University of Science and Technology, Douliu, Yunlin, Taiwan. linchuen@yuntech.edu.tw
Lin
Cheng-Wei
CW
D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D007328:Insulin / Q000008:administration & dosage*; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007004:Hypoglycemic Agents; D007328:Insulin; C514968:carboxymethyl-chitosan; D048271:Chitosan