Cloning, purification and preliminary X-ray crystallographic analysis of a hypothetical protein, MJ0754, from Methanococcus jannaschii DSM 2661.
The protein encoded by the MJ0754 gene from the archaeon Methanococcus jannaschii DSM 2661 is an unknown hypothetical protein. Two recombinant proteins, MJ0754 (residues 1-185) and MJ0754t (a truncated form of MJ0754, residues 11-185), were cloned from MJ0754, overexpressed as His-tag fusion proteins and purified. The crystals were found to grow under two different conditions and to have two different shapes. The crystal of MJ0754 belonged to space group P6(1), with unit-cell parameters a = b = 127.015, c = 48.929 A, a calculated Matthews coefficient of 2.85 A(3) Da(-1) and two molecules per asymmetric unit. The crystal of MJ0754t belonged to space group C222(1), with unit-cell parameters a = 51.915, b = 79.122, c = 93.869 A, a calculated Matthews coefficient of 2.41 A(3) Da(-1) and one molecule per asymmetric unit. The SeMet-labelled P6(1) crystal diffracted to a resolution of 3.1 A, while the native C222(1) crystal diffracted to 1.3 A resolution.
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Eun Hye
EH
Lee
Ki Hyun
KH
Nam
Kwang Yeon
KY
Hwang
D019843:Archaeal Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D017017:Methanococcus / Q000737:chemistry*
D019843:Archaeal Proteins