Scrub typhus induced by peripheral blood stem cell transplantation in the immunocompromised patient: diagnostic usefulness of nested polymerase chain reaction.
Scrub typhus (Orientia tsutsugamushi) is a Gram-negative rickettsial disease in parts of Asia, transmitted from wild rodents to human by mites. This is a case report of scrub typhus contraction in an acute leukemia patient by transfusion of peripheral blood stem cells collected during the incubation period. Although human-to-human transmission of scrub typhus by needle-stick injury or transplacental transmission has previously been reported, this is the first case confirmed by polymerase chain reaction (PCR) and DNA sequencing. This type of incident shows the need to heighten awareness of the threat of rickettsial agents in transfused blood. Nested PCR is a useful diagnostic method to confirm the diagnosis during incubation period and in the early phase of disease, especially for immunocompromised patients.
Kang
Seung-Ji
SJ
Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
Park
Kyung-Hwa
KH
Jung
Sook-In
SI
Jang
Hee Chang
HC
Ji
Soo Young
SY
Ahn
Jae Sook
JS
Kim
Hyeoung Joon
HJ
Shin
Jong-Hee
JH
Kim
Dong Min
DM
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D003951:Diagnostic Errors; D005260:Female; D005334:Fever / Q000209:etiology; D006086:Graft vs Host Disease / Q000175:diagnosis; D006801:Humans; D016867:Immunocompromised Host; D007166:Immunosuppressive Agents / Q000009:adverse effects; D055256:Infectious Disease Incubation Period; D015470:Leukemia, Myeloid, Acute / Q000150:complications* / Q000601:surgery; D019520:Living Donors; D008297:Male; D008969:Molecular Sequence Data; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012612:Scrub Typhus / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014184:Transplantation, Homologous / Q000009:adverse effects
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007166:Immunosuppressive Agents