Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning.
OBJECTIVE
To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats.


METHODS
Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles.


RESULTS
R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05).


CONCLUSIONS
R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.
Surgery and Research Postgraduate Program, UNIFESP, Sao Paulo, Brazil.
Luciana Lamarão
LL
Damous
Sônia Maria da
SM
Silva
Renato Antônio Migliano
RA
Lopes
Célia Regina de Souza Bezerra
CR
Sakano
Manuel de Jesus
Mde J
Simões
Edna Frasson de Souza
EF
Montero
D000818:Animals; D015925:Cryopreservation; D004971:Estrus / Q000502:physiology; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D019194:Ischemic Preconditioning / Q000379:methods*; D006080:Ovarian Follicle / Q000098:blood supply*; D010053:Ovary / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D014021:Tissue Preservation; D014182:Transplantation, Autologous; D014626:Vaginal Smears