Effects of estrogen on PMCA 2 and 4 in human fibroblast-like synovial cells and mouse macrophage-like cells.
We investigated the possible roles of estrogen on plasma membrane Ca(2+)-ATPase (PMCA) in human fibroblast-like synovial cells (HFLS) and mouse macrophage-like cells (RAW 264.7). Western blots revealed the expression of PMCA 2 and 4 in both cells. In vitro treatments with 17beta-estradiol for 24 hours resulted in a concentration dependent decrease in PMCA expression. Moreover, Ca(2+)-ATPase specific activity was similarly decreased with estrogen treatments. However, treatments for 1 hour in the presence or absence of cycloheximide demonstrated non-significant effects. These results suggest that estrogen has a modulatory role on Ca(2+) homeostasis through decreasing PMCA expression and abating their activity.
Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
Waleed
W
El-Beialy
Nadia
N
Galal
Yoshiaki
Y
Deyama
Yoshitaka
Y
Yoshimura
Kuniaki
K
Suzuki
Kanchu
K
Tei
Yasunori
Y
Totsuka
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013582:Synovial Fluid / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D011500:Protein Synthesis Inhibitors; D004958:Estradiol; D003513:Cycloheximide; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium