Response surface analysis for the production of an enantioselective lipase from Aspergillus niger by solid-state fermentation.
The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH(2)P0(4), KH(2)P0(4), MgS0(4)-7H(2)0, CaCl(2), NaCI, and MnS0(4). The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH(2)P0(4), and KH(2)P0(4), which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2P04 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as micromoles of oleic acid released per minute of reaction (micromol/min).
Laboratório de Bioquímica de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil. fabiano.contesini@gmail.com
Fabiano Jares
FJ
Contesini
Vania Castriani Fernades
VC
da Silva
Rafael Ferreira
RF
Maciel
Rosemary Joana
RJ
de Lima
Francisco Fábio Cavalcante
FF
Barros
Patrícia
P
de Oliveira Carvalho
D001234:Aspergillus niger / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D008049:Lipase / Q000096:biosynthesis*; D015233:Models, Statistical
D003470:Culture Media; D008049:Lipase