Nonsteroidal anti-inflammatory drug hypersensitivity syndrome. A multicenter study. I. Clinical findings and in vitro diagnosis.
BACKGROUND
We present the results obtained from the largest series of in vitro diagnostic tests ever reported in patients with clinically validated hypersensitivity to acetylsalicylic acid (ASA)/nonsteroidal anti-inflammatory drugs (NSAID) compared with various categories of controls tolerating ASA/NSAIDs. This multicenter study, which was performed within the framework of the European Network for Drug Allergy (ENDA) group, showed that the basophil activation test (BAT), particularly when used with the 3 NSAIDs aspirin (ASA), diclofenac (DIC), and naproxen (NAP), allows us to confirm the diagnosis of NSAID hypersensitivity syndrome. The results of the cellular allergen stimulation test (CAST) frequently correlate with those of the BAT, although not always. An unexpected finding was that basophil activation by NSAIDs is not an all-or-nothing phenomenon restricted to clinically hypersensitive patients, but that it also occurs in a dose-related manner in some NSAID-tolerant control individuals.Therefore, NSAID hypersensitivity appears as a shift in the normal pharmacological response to NSAIDs. These findings allow us to formulate a new rational hypothesis about the mechanism of NSAID hypersensitivity syndrome, a mechanism that most authors continue to describe as "unknown."


METHODS
We enrolled 152 patients with a history of hypersensitivity to NSAIDs and 136 control participants in 11 different centers between spring 2003 and spring 2006. Flowcytometric BAT was performed.


RESULTS
The most noteworthy results of our study were that 57% of 140 patients presented very clear-cut positive BAT results to multiple NSAIDs, and 16% were entirely negative. In about 27% of cases, positive results were obtained with 1 or 2 concentrations of a single NSAID. There is clearly a correlation between the results of BAT and CAST.


CONCLUSIONS
BAT seems particularly indicated in patients with a clinical history of NSAID intolerance, and in whom a provocation test is not advisable for ethical, clinical, or other reasons. Clear-cut positive results can be considered as confirming a history of NSAID hypersensitivity, although negative results may not exclude it.
Department of Allergology and Clinical Immunology, Clinica Universidad de Navarra, Collaborative center of Pamplona, Spain.
A L
AL
De Weck
M L
ML
Sanz
P M
PM
Gamboa
W
W
Aberer
M
M
Blanca
S
S
Correia
S
S
Erdman
J M
JM
Jermann
G
G
Kanny
M
M
Kowalski
L
L
Mayorga
W
W
Medrala
A
A
Merk
G J
GJ
Sturm
J
J
Sainte-Laudy
M S
MS
Schneider
A
A
Sczczeklik
J M
JM
Weber
A
A
Wedi
D000293:Adolescent; D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects* / Q000276:immunology*; D001241:Aspirin / Q000009:adverse effects / Q000276:immunology; D001491:Basophils / Q000166:cytology / Q000276:immunology; D004008:Diclofenac / Q000009:adverse effects / Q000276:immunology; D004342:Drug Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D015289:Leukotrienes / Q000097:blood; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009288:Naproxen / Q000009:adverse effects / Q000276:immunology; D012372:ROC Curve; D012680:Sensitivity and Specificity; D055815:Young Adult
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015289:Leukotrienes; D004008:Diclofenac; D009288:Naproxen; D001241:Aspirin