Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein.
Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 A. The crystals belonged to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 118.8, b = 118.8, c = 495.1 A, alpha = beta = 90, gamma = 120 degrees .
Kotaka
Masayo
M
School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore. masayo@hku.hk
Dutta
Sujit
S
Lee
Hooi Chen
HC
Lim
Mitchell J M
MJ
Wong
Yeehwa
Y
Rao
Feng
F
Mitchell
Edward P
EP
Liang
Zhao Xun
ZX
Lescar
Julien
J
D003460:Crystallization; D018360:Crystallography, X-Ray; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
D014157:Transcription Factors