THE REACTIONS BETWEEN BACTERIA AND ANIMAL TISSUES UNDER CONDITIONS OF ARTIFICIAL CULTIVATION : III. THE ACTION OF BACTERIAL VACCINES ON TISSUE CULTURES IN VITRO.
These results seem to indicate that many bacteria may be utilized by tissue cells as food for growth or may contain a substance or substances stimulating cell growth or multiplication. This substance is stable and is not destroyed by heating to 100 degrees C. or by long standing. With Micrococcus aureus this action is often neutralized or overcome by a substance inhibitory to growth. The nature of these substances has not yet been determined, though several attempts along this line were made by endeavoring to split the typhoid bacterial substance according to the method of Vaughan See PDF for Structure and testing the poisonous and non-poisonous residues separately. However, at the time too little bacterial substance was used to obtain enough end-products to be of much use, and the products so obtained prevented the plasma from coagulating. Even in the uncoagulated plasma there appeared to be an increase of cells in the cultures with the non-poisonous residue. The author hopes to be able to repeat these tests with split products at a later time when more bacterial substance is available, with the hope of obtaining more definite results.
Laboratory of Hygiene of the University of Pennsylvania, Philadelphia.
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Smyth