Genetic characterization of G3 rotaviruses detected in Italian children in the years 1993-2005.
In recent years an apparent increase in the frequency of detection of G3P[8] rotaviruses has been observed worldwide. Similarly, in Italy G3P[8] strains have been detected sporadically and in a scattered fashion over 20 years, whereas in 2003 and 2005 G3P[8] rotavirus activity increased markedly. By analysis of the VP7, VP4, VP6, and NSP4 genes of a selection of G3P[8] rotaviruses detected between 1993 and 2005, a remarkable sequence conservation was observed in the VP7, VP4, and VP6 genes. By converse, after 2002 the Italian G3P[8] strains were found to possess unique mutations in significant regions of the NSP4 protein.
De Grazia
Simona
S
Dipartimento di Scienze per la Promozione della Salute G. D'Alessandro, Sezione di Microbiologia, Università di Palermo, Palermo, Italy.
Martella
Vito
V
Colomba
Claudia
C
Cascio
Antonio
A
Arista
Serenella
S
Giammanco
Giovanni M
GM
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D036022:Capsid Proteins / Q000032:analysis / Q000235:genetics; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D017423:Sequence Analysis, RNA; D014118:Toxins, Biological / Q000032:analysis / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000235:genetics
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D012367:RNA, Viral; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins