Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes.
Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 micromol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40 degrees C, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.
Wu
Jing
J
Department of Biotechnology, Dong-A University, Busan 604-714, South Korea. gikura@gmail.com
Jung
Byung-Gil
BG
Kim
Kyoung-Sook
KS
Lee
Young-Choon
YC
Sung
Nak-Chang
NC
D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D014320:Triphenylmethyl Compounds / Q000378:metabolism*
D004396:Coloring Agents; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane