Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits.
OBJECTIVE
To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine.


METHODS
Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and
including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method.


RESULTS
Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate.


CONCLUSIONS
The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.
Stem Cell Research Institute, Sao Paulo, Brazil. lilian@ipctron.com.br
Lilian Piñero
LP
Eça
Renata Belmonte
RB
Ramalho
Isis Sousa
IS
Oliveira
Paulo Oliveira
PO
Gomes
Paulo
P
Pontes
Alice Teixeira
AT
Ferreira
Marcelo Paulo Vaccari
MP
Mazzetti
D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004195:Disease Models, Animal; D005269:Femur / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D007085:Ilium / Q000166:cytology; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D044968:Regenerative Medicine / Q000379:methods; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards