Mutations at the same amino acid in myosin that cause either skeletal or cardiac myopathy have distinct molecular phenotypes.
To date, more than 230 disease-causing mutations have been linked to the slow/cardiac muscle myosin gene, beta-MyHC (MYH7). Most of these mutations are located in the globular head region of the protein and result in cardiomyopathies. Recently, however, a number of novel disease-causing mutations have been described in the long, alpha-helical, coiled coil tail region of the beta-MyHC protein. Mutations in this region are of particular interest because they are associated with a multitude of human diseases, including both cardiac and skeletal myopathies. Here, we attempt to dissect the mechanism(s) by which mutations in the rod region of beta-MyHC can cause a variety of diseases by analyzing two mutations at a single amino acid (R1500P and R1500W) which cause two distinct diseases (Laing-type early-onset distal myopathy and dilated cardiomyopathy, respectively). For diseases linked to the R1500 residue, we find that each mutation displays distinct structural, thermodynamic, and functional properties. Both R1500P and R1500W cause a decrease in thermodynamic stability, although the R1500W phenotype is more severe. Both mutations also affect filament assembly, with R1500P causing an additional decrease in filament stability. In addition to furthering our understanding of the mechanism of pathogenesis for each of these diseases, these data also suggest how the variance in molecular phenotype may be associated with the variance in clinical phenotype present with mutations in the beta-MyHC rod.
Department of Molecular, Cellular, and Developmental Biology, 347 UCB, University of Colorado, Boulder, CO 80309, USA.
Thomas Z
TZ
Armel
Leslie A
LA
Leinwand
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D000596:Amino Acids / Q000235:genetics*; D002152:Calorimetry, Differential Scanning; D002311:Cardiomyopathy, Dilated / Q000235:genetics*; D002942:Circular Dichroism; D049310:Distal Myopathies / Q000235:genetics*; D006801:Humans; D008854:Microscopy, Electron; D008954:Models, Biological; D009154:Mutation; D015879:Myosin Subfragments / Q000235:genetics; D009218:Myosins / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D055550:Protein Stability; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D000596:Amino Acids; D015879:Myosin Subfragments; D009218:Myosins