Influence of membrane curvature on the structure of the membrane-associated pleckstrin homology domain of phospholipase C-delta1.
The effects of geometric properties of membranes on the structure of the phospholipase C-delta1 (PLC-delta1) pleckstrin homology (PH) domain were investigated using solid state (13)C NMR spectroscopy. Conformations of the PLC-delta1 PH domain at the surfaces of multilamellar vesicles (MLV), small unilamellar vesicles (SUV), and micelles were examined to evaluate the effects of membrane curvature on the PH domain. An increase in curvature of the water-hydrophobic layer interface hinders membrane-penetration of the amphipathic alpha2-helix of the PH domain that assists the membrane-association of the PH domain dominated by the phosphatidylinositol 4,5-bisphosphate (PIP(2)) specific lipid binding site. The solid state (13)C NMR signal of Ala88 located at the alpha2-helix indicates that the conformation of the alpha2-helix at the micelle surface is similar to the solution conformation and significantly different from those at the MLV and SUV surfaces which were characterized by membrane-penetration and re-orientation. The signal of Ala112 which flanks the C-terminus of the beta5/beta6 loop that includes the alpha2-helix, showed downfield displacement with decrease in the interface curvature of the micelles, SUV and MLV. This reveals that the conformation of the C-terminus of the beta5/beta6 loop connecting the beta-sandwich core containing the PIP(2) binding site and the amphipathic alpha2-helix is sensitive to alterations of the curvature of lipid bilayer surface. It is likely that these alterations in the conformation of the PLC-delta1 PH domain contribute to the regulatory mechanisms of the intracellular localization of PLC-delta1 in a manner dependent upon the structure of the molecular complex containing PIP(2).
Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Kamigori, Hyogo 678-1297, Japan.
Naoko
N
Uekama
Takahiro
T
Aoki
Toshihiro
T
Maruoka
Seiji
S
Kurisu
Akiko
A
Hatakeyama
Satoru
S
Yamaguchi
Masashi
M
Okada
Hitoshi
H
Yagisawa
Katsuyuki
K
Nishimura
Satoru
S
Tuzi
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D001798:Blood Proteins; D008051:Lipid Bilayers / Q000737:chemistry*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry*; D054803:Phospholipase C delta / Q000737:chemistry*; D010750:Phosphoproteins; D017433:Protein Structure, Secondary / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats; D040681:Structural Homology, Protein
D001798:Blood Proteins; D008051:Lipid Bilayers; D019269:Phosphatidylinositol 4,5-Diphosphate; D010750:Phosphoproteins; C039077:platelet protein P47; D054803:Phospholipase C delta; C518026:Plcd1 protein, rat