Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood.
Commitment towards megakaryocyte versus erythroid blood cell lineages occurs in the megakaryocyte-erythroid progenitor, where mutually exclusive expression of either EKLF (Klf1) or Fli1 defines alternative outcomes. Here we show there is a marked increase in the number of circulating platelets in mice lacking the erythroid transcription factor EKLF. In addition, committed erythroid cells retain key signatures of megakaryocytes both on the cell surface and at the mRNA level. We also show that the effect of EKLF on megakaryocyte-erythroid progenitor lineage decision and commitment is cell autonomous in bone marrow reconstitution assays where stem cells lacking EKLF favor the megakaryocyte differentiation pathway. We conclude the megakaryocyte program is aberrantly activated in EKLF null erythroid cells.
Institute for Molecular Bioscience, University of Queensland, St Lucia, 4072 Qld, Australia.
Michael R
MR
Tallack
Andrew C
AC
Perkins
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000097:blood / Q000172:deficiency* / Q000235:genetics*; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic
D051741:Kruppel-Like Transcription Factors; C080774:erythroid Kruppel-like factor