Isolation of measles virus from clinical specimens using B95a and Vero/hSLAM cell-lines.
The clinical presentation of acute measles is normally quite typical, especially in the presence of Koplik's spots, that laboratory test is seldom required to confirm the diagnosis. However, with wide measles vaccination coverage and the extensive use of immunosuppressive chemotherapy, the diagnosis of atypical manifestations of acute measles may require laboratory confirmation. When compared with B95a cell-line, this study shows that the Vero/hSLAM cell-line is sensitive and is recommended for use in the primary isolation of wild-type measles virus from clinical specimens. Throat swab and urine specimens are the clinical specimens of choice and both are recommended for optimal isolation of measles virus from patients suspected of acute measles virus infection.
Makmal Kesihatan Awam Kebangsaan (National Public Health Laboratory), Kementerian Kesihatan, Lot 1853 Kg. Melayu, 47000 Sungai Buloh, Selangor, Malaysia.
C
C
Keniscope
R
R
Juliana
H
H
Subri
S R
SR
Shangari
W A
WA
Wan Nor Azlina
A
A
Hamizah
E E
EE
Emmi
M D
MD
Nor Azlina
I
I
Norizah
K B
KB
Chua
D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D008459:Measles virus / Q000302:isolation & purification*; D010614:Pharynx / Q000821:virology; D014556:Urine / Q000821:virology; D014709:Vero Cells