A simplified, 96-well-adapted, ATP luminescence-based motility assay.
Directional motility assays make use of Boyden chambers or Transwell culture inserts with porous membranes that separate cells seeded in the upper chamber from a chemoattractant supplied in a lower chamber. These assays are often time-consuming and are associated with several limitations due to manual counting and inconsistent results; low signal-to-noise ratio and fluorescence interference; and high cost and the need for specific equipment. Here, we describe a simple, direct, and easy ATP luminescence-based motility assay (ALMA), which can be used for 96-well plate quantification.
Restouin
Audrey
A
Inserm, U891, TrGET, Centre de Recherche en Cancérologie de Marseille, Marseille, France, Institut Paoli-Calmettes, Marseille, France.
Aresta
Sandra
S
Prébet
Thomas
T
Borg
Jean-Paul
JP
Badache
Ali
A
Collette
Yves
Y
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001943:Breast Neoplasms / Q000473:pathology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D049449:Luminescence; D012680:Sensitivity and Specificity
D016895:Culture Media, Serum-Free; D000255:Adenosine Triphosphate