[Effect of soluble total proteins of Zaocys dumnades on interleukin-1beta, tumor necrosis factor-alpha and interleukin-10 expressions in human fibroblast-like synoviocytes in vitro].
OBJECTIVE
To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro.


METHODS
Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect the expressions of IL-1beta, IL-10 and TNF-alpha in the FLS.


RESULTS
The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01).


CONCLUSIONS
The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS.
Wu
He-yong
HY
Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. wuheyong2002@163.com
Li
Juan
J
Li
Ya-ling
YL
D000818:Animals; D001172:Arthritis, Rheumatoid; D002478:Cells, Cultured; D017812:Colubridae; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D008419:Materia Medica / Q000737:chemistry / Q000494:pharmacology*; D061251:Primary Cell Culture; D011506:Proteins / Q000302:isolation & purification; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D053583:Interleukin-1beta; D008419:Materia Medica; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10