CE-ECL detection of gatifloxacin in biological fluid after clean-up using SPE.
A simple, rapid and sensitive CE method has been proposed for the determination of gatifloxacin in biological fluid. This method is based on the ECL reaction of gatifloxacin and tris(2,2'-bipyridyl)ruthenium(II) occurred in the end-column detection cell. Under the optimal conditions, gatifloxacin can be assayed within 10 min over the concentration range of 5.0x10(-8)-5.0x10(-6) g/mL with the theoretical plate numbers of 18,000. The intra-day and inter-day precision of the signal intensity and the migration time shows acceptable reproducibility for the analysis of gatifloxacin. The presented method has been successfully applied to determine the concentrations of gatifloxacin in urine and blood samples after clean-up using C(18) SPE column.
Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, China. fuzf@swu.edu.cn
Zhifeng
Z
Fu
Lin
L
Wang
Cuifang
C
Li
Ying
Y
Liu
Xueting
X
Zhou
Wei
W
Wei
D000328:Adult; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D000077734:Gatifloxacin; D006801:Humans; D049449:Luminescence; D008297:Male; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077734:Gatifloxacin