[Expression of M4 receptor in normal guinea pigs retina].
OBJECTIVE
To detect the expression and distribution of M4 receptor in normal guinea pigs retina.


METHODS
Twenty one normal 4-week-old triad color guinea were selected. Eyes were enucleated. Seven eyes were fixed, paraffin-embedded and cut into sections. Expression and distribution of M4 receptor were detected using immunohisto chemically. The rest eyes were dissected. Tissue of retina were separated. RNA and protein of different tissue were extracted respectively. M4 receptor at mRNA level and protein level were detected by RT-PCR and Western blot methods.


RESULTS
receptor at mRNA level was detected in normal guinea retina, with the amplification product at 221 bp. No product was detected in negative control. M4 receptor at protein level was detected in normal guinea retina, with signal band at 38,000. No signal was detect in negative control. As detected by immunohistochemistry, M4 receptor was detected in RPE cells, photoreceptor layer, outer nuclear layer, inner nuclear layer, most ganglion cells and the junctional zone of inner plexiform layer and inner nuclear layer.


CONCLUSIONS
M4 receptor was expressed in normal guinea retina.
Gao
Yan
Y
The Affliated Hospital of Medical College of Qingdao University, Qingdao 266003, China. gaoyansd@yahoo.com.cn
Zeng
Jun-Wen
JW
Liu
Gui-Xiang
GX
D000818:Animals; D005136:Eye Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D012333:RNA, Messenger / Q000235:genetics; D043588:Receptor, Muscarinic M4 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
D005136:Eye Proteins; D012333:RNA, Messenger; D043588:Receptor, Muscarinic M4