3D environment on human mesenchymal stem cells differentiation for bone tissue engineering.
In this work a novel method was developed to create a three dimensional environment at a cellular level for bone tissue engineering. Biphasic calcium phosphate (BCP) particles of 140-200 microm were used in association with human mesenchymal stem cells (hMSCs). The cells seeded on these particles adhered and proliferated more rapidly in the first day of culture compared to culture on plastic. Analyses of hMSCs cultured without osteogenic factors on BCP particles revealed an abundant extracellular matrix production forming 3-dimensional (3D) hMSCs/BCP particles constructs after few days. Bone morphogenetic 2 (BMP-2), bone sialoprotein (BSP) and ALP gene expression using real time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed that expression profiles were modified by the culture substrate while the addition of osteogenic medium enhanced bone markers expression. These results indicate that BCP particles alone are able to induce an osteoblastic differentiation of hMSCs that might be of interest for bone tissue engineering.
INSERM U791, Center on Osteoarticular and Dental Tissue Engineering, School of Dental Surgery, Nantes, France. thomas.cordonnier@univ-nantes.fr
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Cordonnier
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Layrolle
Julien
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Gaillard
Alain
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Langonné
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Sensebé
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Rosset
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Sohier
D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002130:Calcium Phosphates / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional; D058574:Integrin-Binding Sialoprotein; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D011137:Polystyrenes / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods
D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D011137:Polystyrenes; D012795:Sialoglycoproteins; D017886:Durapatite; C020243:calcium phosphate; D000469:Alkaline Phosphatase