Apple (Malus domestica L. Borkh.) allergen Mal d 1: effect of cultivar, cultivation system, and storage conditions.
It has been reported on the basis of skin prick tests and gene expression studies that apple cultivars differ in their allergenic potential. Only a few studies have tried to measure the amount of the major apple allergen Mal d 1 so far. Mal d 1 belongs to the pathogenesis-related proteins, a family of proteins that are induced by pathogens and environmental stress. Due to cross-reactivity between Bet v 1 and proteins present in several plant-derived foods, birch pollen allergic patients develop food allergies, most frequently to apples. Mal d 1 content was quantified in different apple cultivars, cultivated at the research stations Klein-Altendorf and Bavendorf, dependent on cultivation method and storage conditions by sandwich-ELISA. Apple cultivars differ considerably in their Mal d 1 content. A high variability in Mal d 1 content was determined within one cultivar and between the two locations for the same apple cultivar. In most cases organically cultivated fruit showed lower Mal d 1 content in comparison to fruit from integrated production. At harvest the detected concentration of Mal d 1 was low, but during storage the Mal d 1 content increased significantly.
Institute of Crop Science and Resource Conservation, Horticultural Science, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany. amatthes@uni-bonn.de
Anne
A
Matthes
Michaela
M
Schmitz-Eiberger
D000383:Agriculture / Q000379:methods; D000485:Allergens / Q000032:analysis*; D052179:Antigens, Plant; D004797:Enzyme-Linked Immunosorbent Assay; D005519:Food Preservation / Q000379:methods*; D058870:Food, Organic / Q000032:analysis; D005638:Fruit / Q000254:growth & development* / Q000276:immunology*; D027845:Malus / Q000254:growth & development* / Q000276:immunology*; D010940:Plant Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D013045:Species Specificity
D000485:Allergens; D052179:Antigens, Plant; C095551:MALD1 protein, Malus domestica; D010940:Plant Proteins