Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS.
We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with alpha-keto-gamma-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 +/- 0.3 microM for Trolox, 5.7 +/- 0.3 microM for ascorbic acid, 8.4 +/- 0.4 microM for uric acid and 38 +/- 2 microM for reduced glutathione.
Department of Chemistry, University of Canterbury, Christchurch, New Zealand.
Senti T
ST
Senthilmohan
Brett M
BM
Davis
Paul F
PF
Wilson
Murray J
MJ
McEwan
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood; D002087:Butyrates / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000097:blood; D005978:Glutathione / Q000032:analysis / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds; D014527:Uric Acid / Q000032:analysis / Q000097:blood
D000975:Antioxidants; D002087:Butyrates; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D014527:Uric Acid; C045636:2-keto-4-mercaptobutyric acid; D005978:Glutathione; D001205:Ascorbic Acid