Molecular characterisation of rifampicin-resistant Mycobacterium tuberculosis strains from Morocco.
Mutations in the rpoB gene associated with rifampicin (RMP) resistance were studied in 47 RMP-resistant and 147 RMP-susceptible clinical strains of Mycobacterium tuberculosis from Morocco using probe-based assay and DNA sequencing. RMP-resistant mutations were identified in 85% of RMP-resistant isolates. No mutations were observed among the 147 RMP-susceptible strains. Sequence analysis identified 10 alleles, including two deletions not previously reported. Nucleotide changes at codons 531, 526 and 516 were the most prominent, accounting for 74.4% of our RMP-resistant strains. These results demonstrate that resistance genotyping at these codons would be a good marker for the rapid detection of RMP resistance.
Laboratoire de Biologie Moléculaire, Institut National d'Hygiène, Rabat, Morocco.
M
M
Kourout
I
I
Chaoui
R
R
Sabouni
O
O
Lahlou
M
M
El Mzibri
A
A
Jordaan
T C
TC
Victor
M
M
Akrim
R
R
El Aouad
D000293:Adolescent; D000328:Adult; D000368:Aged; D000904:Antibiotics, Antitubercular / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics*; D003062:Codon; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009018:Morocco; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D012189:Retrospective Studies; D012293:Rifampin / Q000627:therapeutic use*; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000208:ethnology / Q000382:microbiology; D055815:Young Adult
D000904:Antibiotics, Antitubercular; D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin