CR3 (CD11b/CD18) activation of nasal neutrophils: a measure of upper airway endotoxin exposure.
Inhaled endotoxin (lipopolysaccharide, LPS) initiates an inflammatory response and leads to the expression of CR3 (CD11b/CD18) receptors on polymorphonuclear leukocytes (PMNs). We determined if PMN activation in nasal lavage fluid (NLF) is a possible biomarker of occupational endotoxin exposure. Seven subjects exposed to endotoxin provided NLF samples that were split into three aliquots (negative control--1 M nicotinamide; sham; positive control--11 etag of exogenous LPS) and PMN activation was measured using a chemiluminometer. Differences in mean PMN activation were apparent, negative control: 548 +/- 15.65 RLU 100 microl(-1); sham: 11469 +/- 2582 RLU 100 microl(-1); positive control: 42026 +/- 16659 RLU 100 microl (n = 7; p <0.05). This technique shows promise as a diagnostic method for measuring upper airway LPS exposure.
University of Toronto, Toronto, Ontario, Canada. romy.seth@utoronto.ca
Romy
R
Seth
Alexander D
AD
Romaschin
Marcos
M
Ribeiro
Susan M
SM
Tarlo
D000704:Analysis of Variance; D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D017817:Nasal Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009784:Occupational Diseases / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D016273:Occupational Exposure / Q000009:adverse effects
D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen