Analysis of the GIGYF2 gene in familial and sporadic Parkinson disease in the Spanish population.
OBJECTIVE
Linkage analysis in familial Parkinson's disease (PD) identified a locus in 2q36-37 (PARK11). Sequencing of GIGYF2 identified several variants only present amongst PD individuals.


METHODS
We analyzed the presence of disease-associated GIGYF2 variants in familial and sporadic PD from Spanish origin by sequencing of 147 PD individuals. The entire GIGYF2 coding sequence was analyzed in 122 familial PD individuals and exons 2, 4, 8-11, 14 and 25-26 were sequenced in 25 sporadic PD to identify disease-associated variants.


RESULTS
We found no variants associated with PD and failed to identify any of previously PD-associated GIGYF2 variants in our sample. We identified four novel missense changes in GIGYF2. p.Met48Ile was found in a PD individual who also was a carrier of two PARKIN mutations. p.Q1244_Q1247del variant was present only in one PD individual but not found in 70 controls. However, its location in the highly polymorphic GIGYF2 glutamine/proline-rich region does not support a role in PD. Two variants (p.P1238insAGC and p.Q1249del) were present both in PD subjects and in controls. Additionally, the p.L1230_Q1237del variant, which was previously considered as a PD-associated change, was found in one control.


CONCLUSIONS
Our findings suggest that GIGYF2 mutations are not a frequent cause of PD in the Spanish population, since we found no clearly segregating variants. We propose further analyses in PD subjects from different populations to define the role of GIGYF2. A clear pathogenic mutation in other gene at 2q36-37 in the PARK11-linked PD families would definitively disprove GIGYF2 as the responsible gene.
Neurogenetics Laboratory, Division of Neurosciences, Center for Applied Medical Research, University of Navarra, Pamplona, Spain.
L
L
Samaranch
E
E
Lorenzo
M A
MA
Pastor
M
M
Riverol
M R
MR
Luquin
M C
MC
Rodríguez-Oroz
J A
JA
Obeso
P
P
Pastor
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002352:Carrier Proteins / Q000235:genetics*; D002889:Chromosomes, Human, Pair 2; D005091:Exons; D005190:Family; D005260:Female; D056726:Genetic Association Studies; D014644:Genetic Variation; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D010300:Parkinson Disease / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013030:Spain; D044767:Ubiquitin-Protein Ligases / Q000235:genetics; D044465:White People / Q000235:genetics*; D055815:Young Adult
D002352:Carrier Proteins; C478016:GIGYF2 protein, human; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein