Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts.
A new eastern blotting technique has been established for ginsenoside Re (G-Re) contained in American ginseng berry pulp extracts. G-Re in American ginseng berry pulp was extracted using 100% methanol, 100% ethanol, 50% aqueous methanol, and 50% aqueous ethanol. The combined crude extracts were applied onto a polyethersulfone membrane and developed using the methanol-water-acetic acid solvent system (45:55:1 v/v). Separated components were immunostained using anti-G-Re monoclonal antibody. G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp.
Morinaga
Osamu
O
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.
Uto
Takuhiro
T
Yuan
Chun-Su
CS
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000911:Antibodies, Monoclonal; D002855:Chromatography, Thin Layer; D005638:Fruit / Q000737:chemistry*; D036145:Ginsenosides / Q000032:analysis*; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012984:Software
D000911:Antibodies, Monoclonal; D036145:Ginsenosides; D010936:Plant Extracts; C049864:ginsenoside Re