Molecular characterization of Brazilian infectious bursal disease virus isolated from 1997 to 2005.
This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleotide and phylogenetic analysis. All of them had the typical amino acid residues, and all clustered in a phylogenetic tree with the vvIBDV strains. Four amino acid substitutions, at positions D213N, G254D, S317R, and D323E, were common to 3 vv isolates, Br/03/DB, Br/03/CK, and Br/04/CR, and differed from other vv isolates and strains. These isolates came from the same locale, but were collected in different years, indicating that the vvIBDVs circulating on Brazilian farms are undergoing slight but continuous exchanges.
Instituto Biológico, Caixa Postal 12898, São Paulo, SP, Brazil.
Maria Judite B
MJ
Fernandes
Isabela C
IC
Simoni
Maria Gabriela
MG
Vogel
Ricardo
R
Harakava
Eliana B
EB
Rivas
Maria Beatriz C
MB
Oliveira
Ana M I
AM
Kanashiro
Eliana N C
EN
Tessari
Nilce M S Q
NM
Gama
Clarice W
CW
Arns
D000595:Amino Acid Sequence; D000818:Animals; D018175:Birnaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D012189:Retrospective Studies; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics
C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins