Tunicamycin-induced cell death in the trigeminal ganglion is suppressed by nerve growth factor in the mouse embryo.
The effect of nerve growth factor (NGF) on tunicamycin (Tm)-treated neurons in the trigeminal ganglion was investigated by use of caspase-3 immunohistochemistry. In intact embryos at embryonic day 16.5, only a few caspase-3-immunoreactivity were detected in the ganglion neurons. Mean +/- SE of the density of the immunoreactivity was 0.22 +/- 0.03%. In contrast, the number of the immunoreactive neurons was increased at 24 h after injection of 0.5 microg Tm in 1 microl of 0.05 N NaOH solution into mouse embryos at embryonic day 15.5. The density of immunoreactivity was also increased (mean +/- SE = 1.44 +/- 0.11%) compared to intact and 0.05 N NaOH-treated embryos (mean +/- SE = 0.35 +/- 0.03%). The Tm treatment caused increase of the number of trigeminal neurons representing apoptotic profiles (intact, mean +/- SE = 79.3 +/- 8.5; 0.05 N NaOH, mean +/- SE = 132 +/- 11.5; 0.5 microg Tm, mean +/- SE = 370.2 +/- 64.8). In addition, NGF significantly prevented the increase of density of the immunoreactivity (mean +/- SE = 0.54 +/- 0.16%) and the number of apoptotic cells (mean +/- SE = 146.2 +/- 11.3). Saline application (without NGF) had no effect on Tm-induced increase of the immunoreactivity (mean +/- SE = 1.78 +/- 0.23%) or the apoptotic profiles (mean +/- SE = 431.9 +/- 80.5). These results indicate that Tm-induced cell death in the trigeminal ganglion is suppressed by NGF in the mouse embryo.
Division of Oral and Craniofacial Anatomy, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, 980-8575, Japan. hiroichi@anat.dent.tohoku.ac.jp
Hiroyuki
H
Ichikawa
Bing-Ran
BR
Zhao
Mitsuhiro
M
Kano
Yoshinaka
Y
Shimizu
Toshihiko
T
Suzuki
Ruji
R
Terayama
Saburo
S
Matsuo
Tomosada
T
Sugimoto
D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020932:Nerve Growth Factor / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012668:Trigeminal Ganglion / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D014415:Tunicamycin / Q000037:antagonists & inhibitors* / Q000633:toxicity
D000900:Anti-Bacterial Agents; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D009498:Neurotoxins; D014415:Tunicamycin; D020932:Nerve Growth Factor; D053148:Caspase 3