Histochemical analyses of tissue-engineered human menisci.
The field of tissue engineering remains one of the least explored areas of current meniscal research but holds great promise. In this investigation, meniscal fibrochondrocytes were isolated from fresh human meniscal tissue and seeded onto synthetic polyglycolic acid (PGA) scaffolds. Constructs were implanted into the dorsal subcutaneous space of athymic nude mice. Control scaffolds, devoid of meniscal cells, were simultaneously implanted in additional mice. Constructs were harvested over 12 weeks and treated with a variety of histochemical stains to analyze general specimen morphology, cellular viability and proliferation, and collagen secretion. Results indicate that meniscal fibrochondrocyte proliferation increased over the time of implantation with cellular consolidation occurring as the PGA scaffolding was progressively hydrolyzed. Collagen production also increased over time. There were favorable similarities between constructs and human meniscal controls in terms of cellular morphology, phenotypic expression, and collagen production. These initial findings demonstrate procedures supporting proliferation of meniscal fibrochondrocytes, expression of fibrochondral phenotype, and the formation of putative meniscal tissue.
Department of Integrative Medical Sciences, Northeastern Ohio Universities Colleges of Medicine and Pharmacy, Roots town, USA. ajschoen@neoucom.edu
Andrew J
AJ
Schoenfeld
Robin
R
Jacquet
Elizabeth
E
Lowder
Alison
A
Doherty
Mark C
MC
Leeson
William J
WJ
Landis
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D051445:Fibrocartilage / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D006085:Graft Survival / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010641:Phenotype; D011100:Polyglycolic Acid / Q000494:pharmacology / Q000627:therapeutic use; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous / Q000379:methods
D011100:Polyglycolic Acid; D003094:Collagen