A furanocoumarin from Prangos uloptera roots, biological effects.
A known furanocoumarin, 8-geranyloxy psoralen, was isolated from n-hexane extract of Prangos uloptera roots by the TLC method. Its structure was determined by comparison of the spectral data with the literature. Cytotoxic effects of the isolated compound were determined by MTT and Tripan blue assays. The antioxidant and antimicrobial potential of the compound were evaluated by DPPH assay and agar dilution method, respectively. The MTT assay results showed that 8-geranyloxy psoralen reduced cell viability of Hela and Mc-Coy cell lines with IC(50) values of 0.792 and 0.835 mM, respectively. Based on the Tripan blue assay, the compound has cytotoxic effects, with an IC(50) value of 1.26 mM for Mc-Coy cell line. The compound exhibited weak antioxidant potential, with an RC(50) value of 0.262 mg mL(-1) and high antimicrobial effects against Staphylococcus epidermidis and Candida kefyr.
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. razavi694@gmail.com
Seyed Mehdi
SM
Razavi
Saber
S
Zahri
Hossein
H
Nazemiyeh
Gholamreza
G
Zarrini
Sariyeh
S
Mohammadi
Mohammad-Amin
MA
Abolghassemi-Fakhri
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D019661:Apiaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002175:Candida / Q000187:drug effects; D002460:Cell Line; D002855:Chromatography, Thin Layer; D011564:Furocoumarins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006586:Hexanes / Q000737:chemistry; D006801:Humans; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects
D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D011564:Furocoumarins; D006586:Hexanes; D010851:Picrates; D010936:Plant Extracts; C026385:n-hexane; C004931:1,1-diphenyl-2-picrylhydrazyl