Preparation and characterization of porous alginate scaffolds containing various amounts of octacalcium phosphate (OCP) crystals.
The present study was designed to investigate whether the amount of octacalcium phosphate (OCP) affects the characteristics of alginate (Alg)/OCP scaffolds regarding the pore formation and its distribution, and the thermodynamic stability from OCP to hydroxyapatite (HA) in an in vitro physiological environment. Alg/OCP composites with weight ratios of 100/0, 75/25, 50/50, and 25/75 were prepared through mixing the ground synthesized OCP crystals with an Alg solution and applying lyophilization. Analysis of X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, and mercury intrusion porosimetry verified that the crystalline phase of OCP and the porosities were retained regardless of the OCP amount. On the other hand, the elastic modulus, determined by mechanical testing, and, interestingly, the pore size increased with increasing the OCP amount. The immersion of the composites in a simulated body fluid up to 14 days revealed that OCP in Alg matrices tends to convert to HA with enhancing the calcium consumption depending on the OCP amount. The results indicated that the inclusion of OCP crystals in the Alg matrix by the mixing process controls the character of the pore distribution in Alg/OCP composites while maintaining the transitory nature of OCP.
Shiraishi
Naru
N
Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
Anada
Takahisa
T
Honda
Yoshitomo
Y
Masuda
Taisuke
T
Sasaki
Keiichi
K
Suzuki
Osamu
O
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D055119:Elastic Modulus; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D016062:Porosity; D051381:Rats; D013314:Stress, Mechanical; D014961:X-Ray Diffraction
D000464:Alginates; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D006603:Hexuronic Acids; C022045:octacalcium phosphate; D020723:Glucuronic Acid; D017886:Durapatite