[The study of two alkaliphilic thermophile bacteria of the anoxybacillus genus as producers of extracellular proteinase].
Two strains of alkaliphilic thermophile bacteria of the genus Anoxybacillus from hydrothermal vents of Lake Baikal were detected and characterized. It was demonstrated that proteinases secreted by these bacteria had wide substrate specificity, hydrolyzed proteins and n-nitroanilide substrates, and showed maximal activity at pyroglutamyl-alanine-alanine-leucine n-nitroanilide hydrolysis. We determined maximal activity of the proteinases at alkaline pH values (10.0-10.5), the enzymes were thermostable and were characterized by a wide thermal optimum (55-70 degrees C). The results of inhibitor analysis and substrate specifity examination of extracellular enzymes demonstrated their belonging to the subtilisin-like serine proteinases.
E V
EV
Lavrent'eva
A P
AP
Shagzhina
O B
OB
Babasanova
Ia E
IaE
Dunaevskiĭ
Z B
ZB
Namsaraev
D D
DD
Barkhutova
D001406:Bacillaceae / Q000201:enzymology* / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D012800:Siberia; D013379:Substrate Specificity / Q000502:physiology; D014871:Water Microbiology
D012697:Serine Endopeptidases