Interaction of sortase A and lipase 2 in the inhibition of Staphylococcus aureus biofilm formation.
Recombinant sortase A (SrtA) was used to immune rabbit, and the inhibitory activity of anti-SrtA serum on Staphylococcus aureus biofilm formation was tested. Biofilm formation was inhibited by anti-SrtA rabbit serum in S. aureus ATCC25923 and two clinical isolated strains. The antiserum was separated into two fractions, and the main component with the inhibitory activity was demonstrated to be the IgG fraction. Two proteins interact with the IgG fraction were identified by using an in vitro pull-down assay and were confirmed to be lipase 2 and gamma-hemolysin by mass spectrometry. Cross-interaction between SrtA and lipase 2 was further confirmed by Western blotting. Addition of anti-lipase 2 serum in the culture medium also showed inhibitory effect against biofilm formation. Together, our study suggests anti-SrtA serum inhibits S. aureus biofilm formation and lipase 2 is one of the targets of anti-SrtA serum in this inhibition process. This is the first study to demonstrate the roles of antisera against SrtA and lipase 2 in the inhibition of biofilm formation in S. aureus.
State Key Laboratory of Virology, Wuhan Institute of Virology, The Chinese Academy of Sciences, 430071, Wuhan, Hubei Province, China.
Ning
N
Xiong
Chunyan
C
Hu
Yong
Y
Zhang
Shiyun
S
Chen
D019881:Aminoacyltransferases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006460:Hemolysin Proteins / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008049:Lipase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000276:immunology / Q000502:physiology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C037956:gamma-hemolysin, Staphylococcus aureus; D019881:Aminoacyltransferases; C120542:sortase A; D008049:Lipase; C426544:lipase 2, Staphylococcus warneri; D003546:Cysteine Endopeptidases