Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description.
The species Aeromonas simiae was first described on the basis of two strains (CIP 107798(T) and CIP 107797) isolated from faeces of healthy monkeys (M. fascicularis) from Mauritius, which were kept in quarantine in the Centre for Primatology, Louis Pasteur University, Strasbourg, France. In the present study, during a survey to determine the prevalence of Aeromonas at three different pig slaughterhouses at north of Portugal, a single strain MDC2374 (out of 703 isolates) was identified as A. simiae on the basis of 16S rDNA, gyrB and rpoD sequencing, confirming that traditional biochemical approaches are not resolute to identify rarely isolated Aeromonas species. To our knowledge, this is the first time since species description that A. simiae is newly isolated and identified.
Fontes
Maria Conceição
MC
Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.
Saavedra
Maria José
MJ
Monera
Arturo
A
Martins
Conceição
C
Martínez-Murcia
Antonio
A
D000003:Abattoirs; D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D027081:DNA Gyrase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase