Electrical stimulation promotes nerve cell differentiation on polypyrrole/poly (2-methoxy-5 aniline sulfonic acid) composites.
The purpose of this work was to investigate for the first time the potential biomedical applications of novel polypyrrole (PPy) composites incorporating a large polyelectrolyte dopant, poly (2-methoxy-5 aniline sulfonic acid) (PMAS). The physical and electrochemical properties were characterized. The PPy/PMAS composites were found to be smooth and hydrophilic and have low electrical impedance. We demonstrate that PPy/PMAS supports nerve cell (PC12) differentiation, and that clinically relevant 250 Hz biphasic current pulses delivered via PPy/PMAS films significantly promote nerve cell differentiation in the presence of nerve growth factor (NGF). The capacity of PPy/PMAS composites to support and enhance nerve cell differentiation via electrical stimulation renders them valuable for medical implants for neurological applications.
Liu
Xiao
X
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia.
Gilmore
Kerry J
KJ
Moulton
Simon E
SE
Wallace
Gordon G
GG
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D017097:Electric Impedance; D004558:Electric Stimulation; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D051381:Rats; D013451:Sulfonic Acids / Q000737:chemistry*
D000814:Aniline Compounds; D001672:Biocompatible Materials; D011108:Polymers; D011758:Pyrroles; D013451:Sulfonic Acids; C525236:poly(2-methoxyaniline-5-sulfonic acid); C067635:polypyrrole; D020932:Nerve Growth Factor