Imaging and organelle distribution of fluorescent InGaP/ZnS nanoparticles in glial cells.
OBJECTIVE
To assess the effects of oleic acid treatment on subcellular distribution of indium gallium phosphide-zinc sulfide (InGaP/ZnS) nanoparticles in microglia and astrocytes.


METHODS
The extent of colocalization between the nanoparticles and organelles was assessed by confocal microscopy, spectrofluorometry and cell sorting.


RESULTS
Cell treatment with a common fatty acid (oleic acid) within the range of physiological concentrations markedly enhanced the InGaP/ZnS uptake by microglia and afforded their colocalization within lipid droplets/lysosomes but not with mitochondria.


CONCLUSIONS
These results suggest that the availability of mono-unsaturated fatty acids, such as oleic acid, in different cells could significantly alter nanoparticle uptake and localization, which can in turn affect the functions of cells and tissues coexposed to nanoparticles.
Department of Pharmacology & Therapeutics, McGill University, Montreal, Quebec, H3G 1Y6, Canada.
Maik
M
Behrendt
Marinella G
MG
Sandros
R Anne
RA
McKinney
Ken
K
McDonald
Ewa
E
Przybytkowski
Maryam
M
Tabrizian
Dusica
D
Maysinger
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D005708:Gallium / Q000737:chemistry / Q000378:metabolism*; D007204:Indium / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology; D000073718:Pancreatitis-Associated Proteins; D010720:Phosphines / Q000737:chemistry / Q000378:metabolism*; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism*; D017967:Zinc Compounds / Q000737:chemistry / Q000378:metabolism*
D000073718:Pancreatitis-Associated Proteins; D010720:Phosphines; C000612141:REG3A protein, human; D013440:Sulfides; D017967:Zinc Compounds; D007204:Indium; D019301:Oleic Acid; C485338:gallium phosphide; D005708:Gallium; C031238:zinc sulfide; C090882:indium phosphide