A rapid method for detection of PrP by surface plasmon resonance (SPR).
Surface plasmon resonance was used to develop a rapid, label-free and sensitive immunoassay for detection of Prion protein (PrP). Anti-PrP monoclonal antibodies immobilized on the biosensor surface were allowed to bind various concentrations of cellular prion protein (PrP(C)), followed by a pulse with additional soluble anti-PrP polyclonal antibodies to intensify the signal. The interaction of antibody with antigen was monitored in real time. With this method, it was possible to detect PrP(C) with a limit of 31.7 ng/ml in serum and 13.1 ng/ml in HEPES-saline, requiring 1 h for a single sample measurement. The assay also detected misfolded prion protein (PrP(Sc)) in spiked serum and PrP(Sc) in scrapie-infected mouse brains. This is a rapid and sensitive assay for the detection of PrP in serum that could be developed into a platform for a serum-based TSE test.
Jiayu
Wan
W
Institute of Military Veterinary Sciences, Academy of Military Medical Sciences, Changchun, China.
Xiong
Wang
W
Jiping
Li
L
Wensen
Liu
L
Ming
Xu
X
Linna
Liu
L
Jing
Xu
X
Haiying
Wang
W
Hongwei
Gao
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism*; D007118:Immunoassay; D051379:Mice; D018621:PrPC Proteins / Q000097:blood / Q000276:immunology; D018620:PrPSc Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism; D011328:Prions / Q000097:blood / Q000276:immunology; D012608:Scrapie / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D018621:PrPC Proteins; D018620:PrPSc Proteins; D011328:Prions