Induction of chemotaxis to sodium chloride and diacetyl and thermotaxis defects by microcystin-LR exposure in nematode Caenorhabditis elegans.
Apart from the liver disruption, embryotoxicity and genotoxicity, microcystin (MC)-LR also could cause neurotoxicity. Nematode Caenorhabditis elegans was explored as a model to study the neurotoxicity. In the present study, we provided evidence to indicate the neurotoxicity on chemotaxis to NaCl and diacetyl, and thermotaxis from MC-LR exposure to C. elegans. As a result, higher concentrations of MC-LR caused significantly severe defects of chemotaxis to NaCl and diacetyl, and thermotaxis. The neurotoxicity on chemotaxis to NaCl and diacetyl, and thermotaxis from MC-LR exposure might be largely mediated by the damage on the corresponding sensory neurons (ASE, AWA, and AFD) and interneuron AIY The expression levels of che-1 and odr-7 were significantly decreased (P < 0.01) in animals exposed to MC-LR at concentrations lower than 10 microg/L, whereas the expression levels of ttx-1 and ttx-3 could be significantly (P < 0.01) lowered in animals even exposed to 1 microg/L of MC-LR. Moreover, both the chemotaxis to NaCl and diacetyl and the thermotaxis were more significantly reduced in MC-LR exposed mutants of che-1(p674), odr-7(ky4), ttx-1(p767), and ttx-3(ks5) than those in exposed wild-type N2 animals at the same concentrations.
Li
Yunhui
Y
Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China. lyh1216@126.com
Ye
Huayue
H
Du
Min
M
Zhang
Yanfen
Y
Ye
Boping
B
Pu
Yuepu
Y
Wang
Dayong
D
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects*; D003931:Diacetyl / Q000494:pharmacology*; D004785:Environmental Pollutants / Q000633:toxicity; D052998:Microcystins / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D011984:Sensory Receptor Cells / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D004785:Environmental Pollutants; D052998:Microcystins; D012965:Sodium Chloride; C078588:microcystin; D003931:Diacetyl