Immobilization of Escherichia coli cells with cis-epoxysuccinate hydrolase activity for D(-)-tartaric acid production.
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best immobilization conditions optimized by response surface methodology with 83% yield (114 U/g). Cell autolysis was observed after immobilization. Immobilized cells showed high pH (5-10) stability, thermal (up to 65 degrees C) stability, conversion rate (>99.5%), enantioselectivity (ee > 99.6%), and were less affected by metal ions and surfactants compared with free cells. Conversion rate for immobilized cells preserved 93% after 10 repeated batches (5% for free cells).
Pan
Haifeng
H
College of Life Science, Zhejiang University, Hangzhou, 310058, China.
Bao
Wenna
W
Xie
Zhipeng
Z
Zhang
Jianguo
J
Li
Yongquan
Y
D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000502:physiology; D004789:Enzyme Activation; D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D013644:Tartrates / Q000302:isolation & purification* / Q000378:metabolism*
D013644:Tartrates; D006867:Hydrolases; C029768:tartaric acid