LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial Characterization.
THE LOCALIZATION OF CHICK EMBRYONIC LYSOZYME WAS DETERMINED BY TWO TECHNIQUES: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.
Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612.
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Kuettner
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Eisenstein
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Soble
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Arsenis