Multiple layers of B cell memory with different effector functions.
Memory B cells are at the center of longstanding controversies regarding the presence of antigen for their survival and their re-engagement in germinal centers after secondary challenge. Using a new mouse model of memory B cell labeling dependent on the cytidine deaminase AID, we show that after immunization with a particulate antigen, B cell memory appeared in several subsets, comprising clusters of immunoglobulin M-positive (IgM(+)) and IgG1(+) B cells in germinal center-like structures that persisted up to 8 months after immunization, as well as IgM(+) and IgG1(+) B cells with a memory phenotype outside of B cell follicles. After challenge, the IgG subset differentiated into plasmocytes, whereas the IgM subset reinitiated a germinal center reaction. This model, in which B cell memory appears in several layers with different functions, reconciles previous conflicting propositions.
Dogan
Ismail
I
Institut National de la Santé et de la Recherche Médicale U783 'Développement du système immunitaire', Université Paris Descartes, Faculté de Médecine, Site Necker-Enfants Malades, Paris, France.
Bertocci
Barbara
B
Vilmont
Valérie
V
Delbos
Frédéric
F
Mégret
Jérome
J
Storck
Sébastien
S
Reynaud
Claude-Agnès
CA
Weill
Jean-Claude
JC
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D002454:Cell Differentiation; D003564:Cytidine Deaminase; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007156:Immunologic Memory; D051379:Mice; D023421:Models, Animal; D009154:Mutation; D010641:Phenotype
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003564:Cytidine Deaminase