Ozone fumigation (twice ambient) reduces leaf infestation following natural and artificial inoculation by the endophytic fungus Apiognomonia errabunda of adult European beech trees.
In 2006, a controlled infection study was performed in the 'Kranzberger Forst' to address the following questions: (1) Will massive artificial inoculation with Apiognomonia errabunda override the previously observed inhibitory effect of chronic ozone? (2) Can biochemical or molecular markers be detected to account for the action of ozone? To this end six adult beech trees were chosen, three ozone fumigated (2x ozone) and three control trees (ambient = 1x ozone). Spore-sprayed branches of sun and shade crown positions of each of the trees, and uninoculated control branches, were enclosed in 100-L plastic bags for one night to facilitate infection initiation. Samples were taken within a five-week period after inoculation. A. errabunda infestation levels quantified by real-time PCR increased in leaves that were not fumigated with additional ozone. Cell wall components and ACC (ethylene precursor 1-amino cyclopropane-1-carboxylic acid) increased upon ozone fumigation and may in part lead to the repression of fungal infection.
Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
Maren
M
Olbrich
Claudia
C
Knappe
Marion
M
Wenig
Elke
E
Gerstner
Karl-Heinz
KH
Häberle
Mitsutoshi
M
Kitao
Rainer
R
Matyssek
Susanne
S
Stich
Michael
M
Leuchner
Herbert
H
Werner
Katja
K
Schlink
Gerhard
G
Müller-Starck
Gerhard
G
Welzl
Hagen
H
Scherb
Dieter
D
Ernst
Werner
W
Heller
Günther
G
Bahnweg
D000393:Air Pollutants / Q000633:toxicity*; D001203:Ascomycota / Q000187:drug effects*; D029964:Fagus / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*; D015870:Gene Expression / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*
D000393:Air Pollutants; D010126:Ozone