Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment.
A Gram-staining-positive, endospore-forming, motile, rod-shaped bacterium, strain AD-1(T), was isolated from a tidal flat sediment of the coast of Hwangdo on the Yellow Sea, Korea. Strain AD-1(T) grew optimally at pH 7.0-7.5 and 40 degrees C and in the presence of 5-10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD-1(T) was most closely related to Thalassobacillus devorans G-19.1(T) (98.0 % sequence similarity) and Thalassobacillus cyri HS286(T) (97.8 %). The cell-wall peptidoglycan was based on meso-diaminopimelic acid and MK-7 was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The major fatty acids (>10 % of total fatty acids) were iso-C(15 : 0), iso-C(17 : 0) and anteiso-C(15 : 0). The DNA G+C content of strain AD-1(T) was 45.2 mol%. It appears reasonable to classify strain AD-1(T) as a member of the genus Thalassobacillus. There were differences in fatty acid profiles and phenotypic and genetic characteristics between strain AD-1(T) and the type strains of the two Thalassobacillus species. On the basis of the data presented, strain AD-1(T) represents a novel species within the genus Thalassobacillus, for which the name Thalassobacillus hwangdonensis sp. nov. is proposed. The type strain is AD-1(T) (=KCTC 13254(T) =CCUG 56607(T)).
Lee
Soo-Young
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S