Urinary biomarkers suggest that estrogen-DNA adducts may play a role in the aetiology of non-Hodgkin lymphoma.
A variety of evidence suggests that estrogens may induce non-Hodgkin lymphoma (NHL). The reaction of catechol estrogen quinones with DNA to form depurinating estrogen-DNA adducts is hypothesized to initiate this process. These adducts are released from DNA, shed from cells into the bloodstream and excreted in urine. The aim of this study was to determine whether or not the depurinating estrogen-DNA adducts might be involved in the aetiology of human NHL. Estrogen metabolites, conjugates and depurinating DNA adducts were identified and quantified in spot urine samples from 15 men with NHL and 30 healthy control men by using ultraperformance liquid chromatography/tandem mass spectrometry. The levels of estrogen-DNA adducts were significantly higher in the men with NHL than in the healthy control men. Thus, formation of estrogen-DNA adducts may play a critical role in the aetiology of NHL, and these adducts could be potential biomarkers of NHL risk.
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198-6805, USA.
Nilesh W
NW
Gaikwad
Li
L
Yang
Dennis D
DD
Weisenburger
Julie
J
Vose
Cheryl
C
Beseler
Eleanor G
EG
Rogan
Ercole L
EL
Cavalieri
D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000652:urine; D018736:DNA Adducts / Q000737:chemistry / Q000652:urine*; D004967:Estrogens / Q000737:chemistry / Q000652:urine*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000188:drug therapy / Q000652:urine; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D018736:DNA Adducts; D004967:Estrogens; D004247:DNA