Dual effects of hyperbaric oxygen on proliferation and cytotoxic T lymphocyte activity of rat splenic lymphocytes.
Contradictory reports exist regarding the effects of hyperbaric oxygen (HBO2) on immune functions. We hypothesized that the intensity of exposure is a key factor, which leads to the different effects of HBO2 on immunity. In this study, we determined the function of rat splenic lymphocytes after in vitro exposure to different pressure-durations of HBO2. The proliferation rate and total cytotoxic T lymphocyte (CTL) activity stimulated by concanavalin A were enhanced by exposure to low (100 kPa for 60 or 90 minutes and 150 kPa for 30 or 60 minutes) HBO2 pressure-durations, but were inhibited by exposure to higher pressure-durations. We conclude that HBO2 exerts dual effects on lymphocyte function, as appropriate exposure promotes and excessive exposure inhibits the functions.
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, PR China.
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D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D006931:Hyperbaric Oxygenation; D007111:Immunity, Cellular / Q000187:drug effects; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D010100:Oxygen