Protein-loaded microspheres prepared by sequential adsorption of dextran sulphate and protamine on melamine formaldehyde core.
Polyelectrolyte multilayer microspheres were fabricated by layer-by-layer self-assembly of a dextran sulphate and a protamine on melamine formaldehyde cores, followed by the partial decomposition of the core. Effects of pH on the encapsulation of proteins and enzymes with different physico-chemical properties (insulin, aprotinin, peroxidase, glucose oxidase (GOD), catalase (Cat)) in the prepared microspheres were then studied. This method of protein encapsulation demonstrated a high loading capacity and efficiency. The protein incorporation and release was regulated by the pH of the solution. Encapsulated enzymes retained a high specific activity depending on the amount of protein incorporated. Bienzyme system GOD/Cat immobilized in the microspheres was suitable for the glucose content assay.
Chemistry Department, Lomonosov Moscow State University, Moscow, Russia.
Nadezda G
NG
Balabushevich
Natalia I
NI
Larionova
D000327:Adsorption; D016264:Dextran Sulfate / Q000737:chemistry*; D004339:Drug Compounding; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011479:Protamines / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage*; D014227:Triazines / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011479:Protamines; D011506:Proteins; D014227:Triazines; C504353:melamine formaldehyde; D016264:Dextran Sulfate