Efficient conjugation of oligonucleotides through aromatic oxime formation.
The present work reports on the preparation of oligonucleotide conjugates via the formation of aromatic oxime linkage. The conjugation consists in the reaction between the oligonucleotide derivatized at 5'-extremity with a benzaldehyde moiety and an aminooxy reporter group. The conjugation was found highly efficient and was extended for the conjugation of phosphorothioate oligonucleotide. In addition, the stability of the so-formed oxime conjugate was investigated.
Département de Chimie Moléculaire, UMR CNRS 5250, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
Pierre
P
Murat
Nicolas
N
Spinelli
Pascal
P
Dumy
Eric
E
Defrancq
D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010091:Oximes / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010091:Oximes