Monodisperse cell-encapsulating peptide microgel beads for 3D cell culture.
This paper describes a method to produce monodisperse cell-encapsulating microgel beads composed of a self-assembling peptide gel for three-dimensional (3D) cell culture. We used a 3D microfluidic axisymmetric flow-focusing device with an external gelation method. The finely powdered salts were dispersed into a continuous phase, and the salts induced the gelation when in contact with the peptide solution. Over 93% of the cells survived after the encapsulation, and the cells migrated and grew within the gels. Applications of our cell-encapsulating beads include bead-based cell assays in drug testing and engineering tissue constructs.
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
Yukiko
Y
Tsuda
Yuya
Y
Morimoto
Shoji
S
Takeuchi
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005782:Gels / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D013499:Surface Properties
D005782:Gels; D010455:Peptides