STUDIES ON COMPLEMENT FIXATION IN TUBERCULOSIS. III.
The prolonged extraction of the tubercle bacillus with boiling ethyl alcohol, followed by one or more reprecipitations by chilling the hot alcoholic solution, easily yields a preparation very active as antigen in the complement fixation reaction. This preparation gives a precipitation reaction with high dilution of the normal blood serum of a number of species. The precipitation reaction presents as a peculiar feature a very long pro-zone and is further dependent on a preceding heat treatment of the serum for its demonstration. Occurring as a reaction of normal serum, the reaction is apparently not influenced by immunization sufficient to develop moderate specific complement fixation reactions.
Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
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Lewis