Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings.
The modified cellulose solvent- (concentrated phosphoric acid) and organic solvent- (95% ethanol) based lignocellulose fractionation (COSLIF) was applied to a naturally-dry moso bamboo sample. The biomass dissolution conditions were 50 degrees C, 1 atm for 60 min. Glucan digestibility was 88.2% at an ultra-low cellulase loading of one filter paper unit per gram of glucan. The overall glucose and xylose yields were 86.0% and 82.6%, respectively. COSLIF efficiently destructed bamboo's fibril structure, resulting in a approximately 33-fold increase in cellulose accessibility to cellulase (CAC) from 0.27 to 9.14 m(2) per gram of biomass. Cost analysis indicated that a 15-fold decrease in use of costly cellulase would be of importance to decrease overall costs of biomass saccharification when cellulase costs are higher than $0.15 per gallon of cellulosic ethanol.
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA.
Noppadon
N
Sathitsuksanoh
Zhiguang
Z
Zhu
Tsung-Jen
TJ
Ho
Ming-Der
MD
Bai
Yi-Heng Percival
YH
Zhang
D031723:Bambusa / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000191:economics; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D002241:Carbohydrates; D004798:Enzymes; D005936:Glucans; D010756:Phosphoric Acids; D012997:Solvents; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C030242:phosphoric acid; D002480:Cellulase