HPMA-copolymer conjugates targeted to tumor endothelium using synthetic oligopeptides.
Synthesis and characterization of N-(2-hydroxypropyl)methacrylamide (HPMA)-copolymer-based drug carriers targeted on specific receptors in the membrane of endothelial cells by oligopeptides (GRGDG, cyclo(RGDfK), and PHSCN) are described in this study. The copolymers containing targeting oligopeptides bound to the polymer via dodeca(ethylene glycol) spacer showed a receptor-specific time-dependent uptake with selected endothelial cell lines. The polymers were labeled with a fluorescent dye to enable monitoring of the interaction of the polymer conjugate with cells using fluorescence microscopy. Cellular uptake and apoptosis induction have been studied in vitro using various cell lines (EA.hy926, 3T3, SW620, and EL4). In vivo accumulation of the conjugate specifically targeted with cyclo(RGDfK) within the tumor vasculature was detected using fluorescence intravital microscopy in mice. The conjugate targeted by cyclo(RGDfK) was accumulated preferentially in the periphery of the growing tumor suggesting that the cyclo(RGDfK) peptide targets the polymer conjugate to the site of neoangiogenesis, rather than to the tumor mass.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Vídenská, Prague, Czech Republic. pola@imc.cas.cz
Robert
R
Pola
Martin
M
Studenovský
Michal
M
Pechar
Karel
K
Ulbrich
Ondrej
O
Hovorka
David
D
Vetvicka
Blanka
B
Ríhová
D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009389:Neovascularization, Pathologic; D009842:Oligopeptides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D013997:Time Factors
D000178:Acrylamides; D009842:Oligopeptides; C032976:N-(2-hydroxypropyl)methacrylamide