Conversion of peripheral CD4(+)CD25(-) T cells to CD4(+)CD25(+) regulatory T cells by IFN-gamma in patients with Guillain-Barré syndrome.
CD4(+)CD25(-) T cells from 25 Guillain-Barré syndrome (GBS) patients were stimulated by IFN-gamma to generate the induced CD4(+)CD25(+) regulatory T cells (iTregs). Then, their phenotypical and functional characterizations were analyzed. Results show that iTregs can be generated from CD4(+)CD25(-) T cells in GBS patients by IFN-gamma in the presence of activating anti-CD3 and anti-CD28 antibodies in vitro. When CD4(+)CD25(-) T cells are stimulated by 40ng/ml IFN-gamma for 3days, the ratio of conversion is the highest under this condition and iTregs are similar to naturally occurring CD4(+)CD25(+) regulatory T cells of healthy donors phenotypically and functionally.
Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
Shuo
S
Huang
Lei
L
Li
Songlan
S
Liang
Weizhi
W
Wang
D000293:Adolescent; D000328:Adult; D000368:Aged; D000998:Antiviral Agents / Q000494:pharmacology*; D015704:CD4 Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D020275:Guillain-Barre Syndrome / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
D000998:Antiviral Agents; D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D007371:Interferon-gamma