A new Factor Xa inhibitor from Amblyomma cajennense with a unique domain composition.
Bioactive compounds of great interest are found in the saliva of hematophagous organisms. While exploring a cDNA library derived from the salivary glands of the tick Amblyomma cajennense, a transcript that codes for a protein with unique structure (containing an N-terminal Kunitz-type domain and a C-terminus with no homology to any annotated sequences) was found. The recombinant mature form of this protein ( approximately 13.5kDa) was produced in Escherichia coli BL21 (DE3), and it was able to inhibit Factor Xa (FXa) and extend global blood clotting times in vitro and ex vivo. Static and dynamic predictions of its tertiary structure indicate regions that may be related to its FXa inhibitor function.
Laboratório de Bioquímica e Biofísica, Instituto Butantan, Av. Vital Brasil, São Paulo, SP, Brazil.
I F C
IF
Batista
O H P
OH
Ramos
J S
JS
Ventura
I L M
IL
Junqueira-de-Azevedo
P L
PL
Ho
A M
AM
Chudzinski-Tavassi
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015951:Factor Xa / Q000737:chemistry* / Q000378:metabolism; D065427:Factor Xa Inhibitors; D006801:Humans; D026863:Ixodidae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
D018076:DNA, Complementary; D065427:Factor Xa Inhibitors; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D015951:Factor Xa