Identification and quantitation of trace impurities in fatty alcohol ethoxylates using HPLC and MALDI-TOF mass spectrometry.
A two-step HPLC separation that combines isocratic and gradient elution has been developed for the analysis of trace impurities in fatty alcohol ethoxylates (FAEs). Quantitative estimates of the early eluting impurities were obtained from a calibration curve based on the responses of an evaporative light scattering detector (ELSD). Isocratic conditions were used to elute the impurities and gradient conditions for the main FAE components. In this manner, an acceptable compromise was obtained between resolution and analysis time. Fractions were collected and the impurities identified using MALDI-TOF mass spectrometry.
Lee
Dean
D
Laboratory for Advanced Separations Technologies, Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, USA.
Rumbelow
Steve
S
Williams
S Kim Ratanathanawongs
SK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005233:Fatty Alcohols / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry*
D005233:Fatty Alcohols; D013501:Surface-Active Agents; D011092:Polyethylene Glycols