Epidemiology and characterization of Staphylococcus epidermidis isolates from humans, raw bovine milk and a dairy plant.
Geographically related Staphylococcus epidermidis isolates from human patients (n=30), dairy farms (farmers and individual raw milk from cattle, n=36) and a dairy plant (n=55) were examined for epidemiological relatedness by pulsed-field gel electrophoresis and, using in vitro methods, for the ability to produce biofilm and antimicrobial resistance. Methicillin-resistant isolates (MRSE) were also identified and characterized. Isolates from farmers and dairy cattle were found to be genetically related, while isolates from human patients were highly diverse. Some dairy plant isolates (18.2%) were closely related to those from dairy farms. Biofilm production and resistance to antimicrobial agents were most typical for isolates from human patients, of which 76.7% were MRSE. Methicillin resistance was also widespread in farm-related isolates (61.1%). This study indicates the possible transmission of S. epidermidis between cattle and farmers. Dairy products were not proven to be an important source of either human infections or methicillin-resistant strains.
Department of Food and Feed Safety, Veterinary Research Institute, 621 00 Brno, Czech Republic. jaglic@vri.cz
Z
Z
Jaglic
E
E
Michu
M
M
Holasova
H
H
Vlkova
V
V
Babak
M
M
Kolar
J
J
Bardon
J
J
Schlegelova
D000383:Agriculture; D000818:Animals; D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D017720:Molecular Epidemiology; D010641:Phenotype; D013212:Staphylococcus epidermidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial