CONTRIBUTIONS TO THE BIOLOGY OF TISSUE CELLS : I. THE RELATION OF CELL CROWDING TO TISSUE GROWTH IN VITRO.
1. A method has been described by which it is possible to scatter isolated tissue cells in a suitable medium. 2. Experiments were undertaken to determine whether minute fragments of fibroblastic culture are able to persist and multiply when transferred into a new culture medium. 3. Cell divisions were not observed in isolated cells under the conditions of the experiment. Growth took place only when the tissue cells were numerous and close to one another.
Fischer
A
A
Laboratories of The Rockefeller Institute for Medical Research, New York, and the Institute of General Pathology of the University of Copenhagen, Copenhagen, Denmark.