Increased microparticle tissue factor activity in cancer patients with Venous Thromboembolism.
Cancer patients exhibit a high rate of thromboembolism (VTE). In this study, we analyzed levels of microparticle (MP) tissue factor (TF) activity in cancer patients with or without VTE. Blood was collected from cancer patients within 24 h of objectively diagnosed VTE (n=53) and from cancer patients without VTE (n=13). MPs were isolated from platelet poor plasma by centrifugation at 20,000g for 15 min. MP TF activity was measured using a two-stage chromogenic assay. Cancer patients with VTE had a significantly higher mean MP TF activity compared with cancer patients without VTE (1.7+/-3.8 pg/mL vs 0.6+/-0.4 pg/mL, p<0.05). Further prospective studies are required to determine if levels of MP TF activity may be a useful biomarker to identify patients at increased risk for VTE.
Division of Hematology/Oncology, Department of Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7035, USA.
David A
DA
Manly
Jianguo
J
Wang
Sam L
SL
Glover
Raj
R
Kasthuri
Howard A
HA
Liebman
Nigel S
NS
Key
Nigel
N
Mackman
D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D002498:Centrifugation; D002863:Chromogenic Compounds; D003110:Colonic Neoplasms; D006801:Humans; D008175:Lung Neoplasms; D009369:Neoplasms / Q000097:blood / Q000150:complications*; D010190:Pancreatic Neoplasms; D013925:Thromboplastin / Q000032:analysis*; D054556:Venous Thromboembolism / Q000097:blood* / Q000209:etiology
D015415:Biomarkers; D002863:Chromogenic Compounds; D013925:Thromboplastin