[Changes and mechanism of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine].
This study was purposed to investigate the changes of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine, and its possible mechanism. JM cells was induced with 0.4 mg/ml matrine for 4 days, the total RNA was extracted from JM cells before and after matrine induction, the differential expression of apoptosis-related genes were screened with cDNA Expression Array Kit, the expression change of a part of gene was checked by Western blot. The results indicated that after induction of JM cells with matrine, differential expression of 31 genes were found by gene chip hybridization, the expression of caspase 8 was up-regulated more than 5 times. Western blot analysis showed that the up-regulation of caspase 8 gene expression positively correlated with induction time. It is concluded that differential expressions of many apoptosis-related genes in JM cells can be induced by matrine, in which gene expression of caspase 8 is up-regulated notably.
Zhang
Yong-Qing
YQ
Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China. zyqlhj@fmmu.edu.cn
Huang
Gao-Sheng
GS
Chen
Xie-Qun
XQ
Bai
Qing-Xian
QX
Liang
Rong
R
Feng
Ji-Liang
JL
Wang
Zhe
Z
D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000235:genetics; D011807:Quinolizines / Q000494:pharmacology*; D015854:Up-Regulation; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; C505530:CASP8 protein, human; D053181:Caspase 8; D000093842:Matrines