Ectopic expression of hC/EBPs in breast tumor cells induces apoptosis.
CCAAT/enhancer binding proteins (C/EBPs) are a group of transcription factors which have been implicated in cellular proliferation, terminal differentiation, and apoptosis in a variety of tissues including mammary gland. Owing to its role in various cellular functions, inactivation of C/EBP proteins is central to the pathogenesis of many disorders. Recent reports suggest that expression as well as function of C/EBP proteins is deregulated in breast tumors. Although, role of C/EBPs in growth arrest in mammary tissues has been studied in much detail; their role in apoptosis is relatively less explored. In the present study, we have assessed if breast tumors evade apoptosis and grow faster by down regulating and inhibiting the C/EBP proteins, C/EBPalpha in particular. Our data shows that ectopic expression of human C/EBPs in breast tumor cells inhibits proliferation and induces apoptosis which is likely to be associated with caspase dependent pathway.
Drug Target Discovery and Development Division (DTDD), Central Drug Research Institute, CDRI, Chhattar Manzil, Lucknow, UP, India.
Pooja
P
Pal
Savita
S
Lochab
Jitendra
J
Kanaujiya
Sabyasachi
S
Sanyal
Arun Kumar
AK
Trivedi
D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000502:physiology; D049109:Cell Proliferation; D053938:DNA Fragmentation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology
D051017:Apoptosis Regulatory Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D011065:Poly(ADP-ribose) Polymerases