Kinetics determination of electrogenerated hydrogen peroxide (H2O2) using carbon fiber microelectrode in electroenzymatic degradation of phenolic compounds.
The kinetics of electrogenerated hydrogen peroxide (H(2)O(2)), which can activate peroxidases in an electroenzymatic process, was examined by an amperometric technique using a carbon fiber microelectrode that was modified by polyaniline (PAn) film and platinum particles. The electrogeneration of H(2)O(2) was found to be dependent on the pH and applied potential, and resulting in a variable current response of the carbon fiber microelectrode. The highest amount of H(2)O(2) was electrogenerated when 2.3 V was applied between the Pt/Ti anode and a reticulated vitreous carbon (RVC) cathode at pH 6.0, with a current response of 0.0190 microA min(-1). Phenol was completely degraded by the electroenzymatic reaction of the immobilized horseradish peroxidase (HRP), and the time required for the electrogeneration of H(2)O(2) increased according to the initial concentration of phenol. The degradation stoichiometric ratio between the electrogenerated H(2)O(2) and the aqueous phenol under HRP immobilized on RVC was found to be 1:1.
Cho
Seung-Hee
SH
Animal Environment Division, National Institute of Animal Science, Rural Development Administration, 77 Chuksan-gil, Kwonsun-Gu, Suwon 441-706, Republic of Korea.
Jang
Am
A
Bishop
Paul L
PL
Moon
Seung-Hyeon
SH
D000814:Aniline Compounds / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008839:Microelectrodes; D019800:Phenol / Q000737:chemistry* / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D019800:Phenol; D002244:Carbon; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase