Use of ion mobility mass spectrometry and a collision cross-section algorithm to study an organometallic ruthenium anticancer complex and its adducts with a DNA oligonucleotide.
We report the development of an enhanced algorithm for the calculation of collision cross-sections in combination with Travelling-Wave ion mobility mass spectrometry technology and its optimisation and evaluation through the analysis of an organoruthenium anticancer complex [(eta6-biphenyl)Ru(II)(en)Cl]+. Excellent agreement was obtained between the experimentally determined and theoretically determined collision cross-sections of the complex and its major product ion formed via collision-induced dissociation. Collision cross-sections were also experimentally determined for adducts of this ruthenium complex with the single-stranded oligonucleotide hexamer d(CACGTG). Ion mobility tandem mass spectrometry measurements have allowed the binding sites for ruthenium on the oligonucleotide to be determined.
Williams
Jonathan P
JP
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. J.P.Williams@warwick.ac.uk
Lough
Julie Ann
JA
Campuzano
Iain
I
Richardson
Keith
K
Sadler
Peter J
PJ
D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium; D017975:Ruthenium Compounds / Q000737:chemistry*
D000970:Antineoplastic Agents; D018736:DNA Adducts; D009841:Oligonucleotides; D009942:Organometallic Compounds; D017975:Ruthenium Compounds; D012428:Ruthenium