Microencapsulation in genipin cross-linked gelatine-maltodextrin improves survival of Bifidobacterium adolescentis during exposure to in vitro gastrointestinal conditions.
To improve survival during exposure to adverse conditions, probiotic Bifidobacterium adolescentis 15703T cells were encapsulated in novel mono-core and multi-core phase-separated gelatine-maltodextrin (GMD) microspheres where the gelatine (G) phase was cross-linked with genipin (GP). Microscopy showed that encapsulated cells were exclusively associated with maltodextrin (MD) core(s). Small (average diameter 37 microm) and large (70 microm) GMD and G microspheres were produced by modulating factors (e.g. mixing speed, surfactant, GP and G concentrations) affecting the size, structural stability and phase-separation. In vitro sequential gastro-intestinal (GI) juice challenge experiments revealed increased survival of cells encapsulated in GMD ( approximately 10(6-7) cfu mL(-1)) and G (approximately 10(5) cfu mL(-1)) microspheres as compared to free cells (approximately 10(4) cfu mL(-1)). In GMD microspheres, the bacteria derive energy from MD to survive during exposure to acid and bile salts. In conclusion, the novel food grade GMD microencapsulation formulation was shown to protect probiotic bifidobacteria from adverse conditions.
Food Science and Chemical Engineering Programs, Department of Process Engineering and Applied Science, Dalhousie University, Halifax, Nova Scotia, Canada.
Antonela D
AD
Borza
Nana T
NT
Annan
Debra L
DL
Moreau
Paula M
PM
Allan-Wojtas
Amyl
A
Ghanem
Dérick
D
Rousseau
Allan T
AT
Paulson
Lisbeth Truelstrup
LT
Hansen
D001644:Bifidobacterium / Q000166:cytology*; D018914:Cells, Immobilized / Q000166:cytology; D003432:Cross-Linking Reagents / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D005750:Gastric Juice / Q000378:metabolism / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology; D005780:Gelatin / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000737:chemistry*; D039823:Iridoids; D044367:Phase Transition; D011134:Polysaccharides / Q000737:chemistry*; D019936:Probiotics
D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D011134:Polysaccharides; C008315:maltodextrin; D005780:Gelatin; C007834:genipin