Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata.
Telomerase, the key enzyme essential for the maintenance of eukaryotic chromosome ends, contains a reverse transcriptase and an RNA that provides the template for the synthesis of telomeric repeats. Here, we characterize the telomerase subunits in the hemiascomycete yeast Candida glabrata. We propose a secondary structure model for the telomerase RNA that is the largest described to date. Telomerase deletion mutants show a progressive shortening of telomeres and a modest loss of viability. Frequent post-senescence survivors emerge that possess long telomeric repeat tracts. We suggest that the high telomere length heterogeneity accounts for this distinct senescence phenotype.
Kachouri-Lafond
R
R
Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 67084 Strasbourg, France.
Dujon
B
B
Gilson
E
E
Westhof
E
E
Fairhead
C
C
Teixeira
M T
MT
D001483:Base Sequence; D015139:Blotting, Southern; D041221:Candida glabrata / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division; D004271:DNA, Fungal / Q000235:genetics; D005434:Flow Cytometry; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D019098:Telomerase / Q000737:chemistry / Q000235:genetics*; D016615:Telomere / Q000235:genetics*
D004271:DNA, Fungal; D012331:RNA, Fungal; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase