Conversion of alpha-amyrin into centellosides by plant cell cultures of Centella asiatica.
Plant cell cultures of Centella asiatica produce small quantities of centellosides: madecassosid > asiaticosid > madecassic acid > asiatic acid. To obtain a more efficient production system of these bioactive triterpenoid compounds, we developed a process where the substrate, alpha-amyrin, was converted into centellosides by cell suspensions of C. asiatica. When alpha-amyrin in acetone was added at 0.01 mg/ml(-1) to the culture medium, together with the permeabilizing agent DMSO, after 7 days nearly 50% had penetrated the plant cells, of which almost 84% was transformed into centellosides. The system therefore efficiently converts alpha-amyrin into centellosides, thus opening a new possibility for the production of these compounds.
Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, 08028 Barcelona, Spain.
Liliana
L
Hernandez-Vazquez
Mercedes
M
Bonfill
Elisabeth
E
Moyano
Rosa M
RM
Cusido
Arturo
A
Navarro-Ocaña
Javier
J
Palazon
D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival; D028041:Centella / Q000378:metabolism*; D050178:Monoglycerides / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
D050178:Monoglycerides; D009828:Oleanolic Acid; C036380:beta-amyrin