Simple binary optical elements for aberration correction in confocal microscopy.
When a confocal fluorescence microscope with a high-numerical-aperture oil-immersion objective is focused deep into an aqueous medium, aberrations result that degrade image quality. We have designed and fabricated a simple two-level binary phase mask that partially corrects these aberrations, improving axial resolution. We present the design and some confirming results.
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CK
Sieracki
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CG
Levey
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Hansen