Isoelectric focusing, blotting and probing methods for detection and identification of monoclonal proteins.
Isoelectric focusing (IEF) is a technique of exquisite resolution and high sensitivity. When applied to human biological fluids using conventional protein stains it is capable of detecting down to about 100 mg of protein/L. When combined with blotting and probing techniques it can get down to less than 1 mg/L. The exquisite resolution enables a greater discrimination between the various immunoglobulin abnormalities encountered in the clinical laboratory.
Cornell
Findley N
FN
Iso Laboratories Pty Ltd, Balwyn North, Vic. 3104, Australia. danc@helena.com.au