Aluminum-induced oxidative stress in lymphocytes of common carp (Cyprinus carpio).
Several studies of fish have shown that aluminum may induce hypoxia, hypercapnia, metabolic acidosis, and respiratory failure. In lymphocytes, morphologic abnormalities and reduced immune activity have been observed. Nevertheless, there is little data on oxidative stress and such data are essential in order to identify its mechanism of action. The common carp Cyprinus carpio, an omnivorous fish commonly used in commercial aquaculture, has been proposed as a test organism in toxicologic assays due to its economic importance and wide geographic distribution. The aim of this work was to evaluate Al-induced oxidative stress in lymphocytes of the common carp C. carpio. Specimens were exposed to three different concentrations of Al (0.05, 120, and 239 mg/l) in a static exposure system for 96 h. At the end of the exposure period, blood was collected and lymphocytes were separated. Lipid peroxidation, oxidized protein content and the activity of superoxide dismutase, catalase, and glutathione peroxidase were measured. Results show that the tested Al concentrations modified the activity of antioxidant enzymes and elicited higher levels of lipid peroxidation and oxidized proteins. The degree of damage induced was concentration and tissue dependent.
Escuela Nacional de Ciencias Biológicas, Laboratorio de Toxicología Acuática, Departamento de Farmacia, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Col. Industrial Vallejo, Mexico, DF, Mexico.
Sandra
S
García-Medina
Amparo Celene
AC
Razo-Estrada
Leobardo Manuel
LM
Gómez-Oliván
Araceli
A
Amaya-Chávez
Eduardo
E
Madrigal-Bujaidar
Marcela
M
Galar-Martínez
D000535:Aluminum / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D002347:Carps; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute
D000535:Aluminum; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase