Human papillomavirus types in invasive cervical cancer specimens from Turkey.
The main aim of the study is to describe the human papillomavirus (HPV) type-specific distribution in invasive cervical cancer (ICC) specimens from Turkey. Paraffin-embedded ICC specimens were identified from the histopathologic archives of the Hacettepe University Medical School in Turkey. HPV detection was carried out through amplification of HPV DNA by a SPF-10 broad-spectrum primer polymerase chain reaction and subsequently followed by DNA enzyme immunoassay and genotyping by LiPA25 (version 1). Two hundred seventy-seven ICC cases diagnosed between 1993 and 2004 were retrieved. After histologic evaluation and human beta-globin gene analysis for sample quality, 248 cases were considered suitable for HPV/DNA testing. HPV prevalence was 93.5% (232/248; 95% confidence interval: 90.5%-96.6%). The five most common HPV types identified as single types among HPV-positive cases were HPV16 (64.7%), HPV18 (9.9%), HPV45 (9.9%), HPV31 (3.0%), and HPV33 (2.2%). The study shows that in Turkey, HPV16/HPV18 accounted for 75.4% (95% confidence interval: 69.9%-81.0%) of HPV-positive ICC cases. This information is essential to evaluate the potential impact of the HPV vaccines in the country.
Usubütün
Alp
A
Medical School, Department of Pathology, Hacettepe University, Sihhiye, Ankara, Turkey. ausubutu@hacettepe.edu.tr
Alemany
Laia
L
Küçükali
Türkan
T
Ayhan
Ali
A
Yüce
Kunter
K
de Sanjosé
Silvia
S
Font
Rebeca
R
Lloveras
Belen
B
Klaustermeier
Joellen
J
Quint
Wim
W
Muñoz
Nubia
N
Bosch
Francesc Xavier
FX
D003430:Cross-Sectional Studies; D005260:Female; D052162:Human papillomavirus 16; D052161:Human papillomavirus 18; D006801:Humans; D030361:Papillomavirus Infections / Q000150:complications* / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D014421:Turkey; D002583:Uterine Cervical Neoplasms / Q000821:virology*