A marine sulfate-reducing bacterium producing multiple antibiotics: biological and chemical investigation.
A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 degrees C to 30 degrees C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.
Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, 116028 Dalian, China. zhangyi@djtu.edu.cn
Yi
Y
Zhang
Jun
J
Mu
Xiaojie
X
Gu
Chenyan
C
Zhao
Xiaoliang
X
Wang
Zeping
Z
Xie
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045285:Desulfovibrio desulfuricans / Q000378:metabolism*; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D009761:Nystatin / Q000494:pharmacology; D010795:Phthalic Acids / Q000302:isolation & purification / Q000493:pharmacokinetics; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism; D013941:Thymine / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D010795:Phthalic Acids; D009761:Nystatin; D000667:Ampicillin; D013941:Thymine; C028577:monobutyl phthalate