Panaxatriol saponins extracted from Panax notoginseng induces thioredoxin-1 and prevents 1-methyl-4-phenylpyridinium ion-induced neurotoxicity.
OBJECTIVE
Thioredoxin-1 has various biologic activities, including the control of redox balance and the inhibition of apoptosis. The current study was designed to examine the effects of panaxatriol saponins (PTS) extracted from Panax notoginseng on thioredoxin-1 expression and 1-methyl-4-phenylpyridinium ion-induced injury.


METHODS
Using PC12 cells and Kunming mice, we test thioredoxin-1 expression after PTS treatment by Western blot. The protective effect of PTS against 1-methyl-4-phenylpyridinium ion-induced injury was assessed by MTT assay and LDH release assay.


RESULTS
PTS induced thioredoxin-1 expression in vitro and in vivo, and attenuated 1-methyl-4-phenylpyridinium ion-induced cell death of PC12 cells.


CONCLUSIONS
PTS is a new inducer of thioredoxin-1 and has a possible potential as a therapeutic agent for neurodegenerative diseases including Parkinson's disease.
Luo
Fu-Cheng
FC
College of Life Science and Technology, Kunming University of Science and Technology, No 296 Bailong Temple, Kunming 650224, China.
Wang
Sheng-Dong
SD
Li
Kui
K
Nakamura
Hajime
H
Yodoi
Junji
J
Bai
Jie
J
D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005260:Female; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D016716:PC12 Cells; D053723:Panax notoginseng; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013879:Thioredoxins / Q000819:agonists / Q000096:biosynthesis*
D036145:Ginsenosides; D010936:Plant Extracts; D012503:Saponins; D013879:Thioredoxins; C004563:panaxatriol; D015655:1-Methyl-4-phenylpyridinium