Enterobactin synthetase-catalyzed formation of P(1),P(3)-diadenosine-5'-tetraphosphate.
The EntE enzyme, involved in the synthesis of the iron siderophore enterobactin, catalyzes the adenylation of 2,3-dihydroxybenzoic acid, followed by its transfer to the phosphopantetheine arm of holo-EntB, an aryl carrier protein. In the absence of EntB, EntE catalyzes the formation of Ap(4)A, a molecule that is implicated in regulating cell division during oxidative stress. We propose that the expression of EntE during iron starvation produces Ap(4)A to slow growth until intracellular iron stores can be restored.
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Alison L
AL
Sikora
Sean M
SM
Cahill
John S
JS
Blanchard
D000255:Adenosine Triphosphate / Q000378:metabolism; D055162:Biocatalysis; D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D025461:Feedback, Physiological / Q000502:physiology; D006867:Hydrolases / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009097:Multienzyme Complexes / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D015226:Dinucleoside Phosphates; D029968:Escherichia coli Proteins; D062385:Hydroxybenzoates; D009097:Multienzyme Complexes; C020733:diadenosine tetraphosphate; C009135:2,3-dihydroxybenzoic acid; D000255:Adenosine Triphosphate; D006867:Hydrolases; C064012:isochorismatase; D008025:Ligases; C066234:enterobactin synthetase; C061746:2,3-dihydroxybenzoate-AMP ligase, E coli; D020156:Salicylic Acid