Facile synthesis of 4-mercaptophenylboronic acid functionalized gold nanoparticles for selective enrichment of glycopeptides.
In the work, 4-mercaptophenylboronic acid (4-MPBA)-functionalized gold nanoparticles were synthesized via a facile approach. At first, gold nanoparticles (about 50 nm) were prepared by a simple and convenient hydrothermal method based on a polyol process. Then, gold nanoparticles were modified with 4-MPBA by the well-known reaction of Au with the thiol groups. The MPBA-functionalized gold nanoparticles were characterized by Fourier transform infrared spectra and UV/Vis adsorption spectra. Due to the fact that the boronic acid group on the surface of 4-MPBA-modified gold particles can form tight yet reversible covalent bonds with glycopeptides containing cis-1,2-diols groups, the MPBA-modified gold nanoparticles were successfully applied to selective enrichment of glycopeptides. Isolation and enrichment of glycopeptides in a standard protein (asialofetuin and horseradish peroxidase) digestion and a complex sample were performed using MPBA-modified gold nanoparticles, followed by matrix-assisted laser desorption/ionization quadruple ion trap time-of-flight (MALDI-QIT-TOF) mass spectrometric analysis. The experimental results demonstrated that MPBA-modified gold nanoparticles synthesized by the facile approach have the powerful potential for selective enrichment of glycopeptides, and can be an alternative tool in glycoproteomics.
Department of Chemistry and Institutes of Biomedical Sciences, Shanghai 200433, China.
Guoping
G
Yao
Huaiyuan
H
Zhang
Chunhui
C
Deng
Haojie
H
Lu
Xiangmin
X
Zhang
Pengyuan
P
Yang
D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D006020:Glycopeptides; D013438:Sulfhydryl Compounds; D006046:Gold