The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions.
Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.
Michel
B
B
Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Leroy
B
B
Stalin Raj
V
V
Lieffrig
F
F
Mast
J
J
Wattiez
R
R
Vanderplasschen
A F
AF
Costes
B
B
D000818:Animals; D002347:Carps / Q000821:virology; D002478:Cells, Cultured; D004266:DNA Virus Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D015678:Viral Structural Proteins