Effect of smoking on rat basilar artery: correlation with inducible nitric oxide synthase and endothelin converting enzyme-1.
OBJECTIVE
Smoking is an extremely important risk factor for subarachnoid hemorrhage and seems to increase rupture risk of unruptured aneurysms by accelerating their growth rate. The aim of the study was to investigate the effect of smoking on the luminal diameter with wall thicknesses of rat basilar arteries and to detect alterations of inducible nitric oxide synthase and endothelin-converting enzyme -1 in the endothelial cells.


METHODS
Rats were divided into two groups. The level of middle pons slices were embedded in paraffin before they were stained with hematoxylin and eosin. Rabbit anti-human inducible nitric oxide synthase and endothelin converting enzyme-1 antibodies were used.


RESULTS
Significant decrease of the vessel luminal diameter and increase of the vessel wall thickness were found in chronic smokers in our study. There was a linear and significant (p= 0,023, r =0,704) correlation between thickness of the wall and endothelin converting enzyme-1 immune reaction. Correlation was not found with inducible nitric oxide synthase (p > 0.05).


CONCLUSIONS
This study on the comparison of vessel luminal diameter and vessel wall thickness with inducible nitric oxide synthase and endothelin converting enzyme-1 immune reactions revealed that the main effect of smoking on the vessel wall is associated with endothelin converting enzyme-1.
Koc
Kenan
K
Kocaeli University School of Medicine, Neurosurgery Department, Kocaeli, Turkey. kenankoc01@yahoo.com
Anik
Ihsan
I
Bozkurt
Suheyla Uyar
SU
Ercin
Cengiz
C
Ceylan
Savas
S
D000818:Animals; D000906:Antibodies; D016282:Aspartic Acid Endopeptidases / Q000276:immunology / Q000378:metabolism*; D001488:Basilar Artery / Q000201:enzymology*; D002908:Chronic Disease; D000072396:Endothelin-Converting Enzymes; D007150:Immunohistochemistry; D008297:Male; D008666:Metalloendopeptidases / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000453:epidemiology / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000453:epidemiology / Q000378:metabolism*; D020301:Vasospasm, Intracranial / Q000453:epidemiology / Q000378:metabolism
D000906:Antibodies; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes