Influence of age on clock gene expression in peripheral blood cells of healthy women.
Recent studies have demonstrated a close relationship between circadian clock function and the development of obesity and various age-related diseases. In this study, we investigated whether messenger RNA (mRNA) levels of clock genes are associated with age, body mass index, blood pressures, fasting plasma glucose, or shift work. Peripheral blood cells were obtained from 70 healthy women, including 25 shift workers, at approximately 9:00 AM. Transcript levels of clock genes (CLOCK, BMAL1, PER1, and PER3) were determined by real-time quantitative polymerase chain reaction. Stepwise multiple regression analysis demonstrated that BMAL1 mRNA levels were correlated only with age (beta = -.50, p < .001). In contrast, PER3 levels were correlated with fasting plasma glucose (beta = -.29, p < .05) and shift work (beta = .31, p < .05). These results suggest that increased age, glucose intolerance, and irregular hours independently affect the intracellular clock in humans.
Department of Pharmacology, School of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.
Hitoshi
H
Ando
Kentarou
K
Ushijima
Masafumi
M
Kumazaki
Toshinari
T
Takamura
Noritsugu
N
Yokota
Tetsuo
T
Saito
Shin
S
Irie
Shuichi
S
Kaneko
Akio
A
Fujimura
D056930:ARNTL Transcription Factors / Q000097:blood / Q000235:genetics; D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood* / Q000235:genetics; D001683:Biological Clocks / Q000235:genetics*; D001773:Blood Cells / Q000502:physiology*; D015992:Body Mass Index; D056926:CLOCK Proteins / Q000097:blood / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics; D056950:Period Circadian Proteins / Q000097:blood / Q000235:genetics; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D012016:Reference Values; D012189:Retrospective Studies; D055815:Young Adult
D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; C539022:PER1 protein, human; C512644:PER3 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D056926:CLOCK Proteins