[Effects of hyperbaric oxygen on tumor growth in the mouse model of LNCaP prostate cancer cell line].
OBJECTIVE
To assess the safety of hyperbaric oxygen in the treatment of radiation-induced hemorrhagic cystitis in patients with prostate cancer, and to investigate its effect on the growth of indolent prostate cancer in vivo.


METHODS
Thirty severe combined-immunodeficient mice received subcutaneous injection of human prostate cancer LNCaP cells. Then they were randomized to an experimental and a control group and exposed to 20 sessions of hyperbaric oxygen and normobaric air, respectively, followed by a 4-week observation on the growth of the transplanted tumors and analyses of their histopathological features at 28 days, including the volume, microvessel density (CD34), apoptosis markers (p53 and p27 proteins) and the proliferation index (Ki-67) of the LNCaP tumors.


RESULTS
On the 28th day after tumor vaccination, the tumor volume was (120 +/- 7.9) mm3 in the HBO and (122 +/- 8.2) mm3 in the control group; the microvessel density and the expressions of Ki-67, p53 and p27 were 39.3 +/- 5.2, (78.1 +/- 7.6)%, (40.4 +/- 6.2)% and (63.7 +/- 5.1)% in the former, and 36.2 +/- 4.9, (75.3 +/- 8.4)%, (44.2 +/- 5.7)% and (61.5 +/- 5.5)% in the latter. There were no significant differences in all the indexes above between the two groups (P > 0.05).


CONCLUSIONS
Hyperbaric oxygen did not promote the growth of indolent prostate cancer in the murine model, nor did it have any significant effect on the new vessels.
Department of Urology, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. ttanghao@hotmail.com
Hao
H
Tang
Zheng-Yu
ZY
Zhang
Jing-Ping
JP
Ge
Wen-Quan
WQ
Zhou
Jian-Ping
JP
Gao
D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011471:Prostatic Neoplasms; D023041:Xenograft Model Antitumor Assays