Diversity of human parechoviruses isolated from stool samples collected from Thai children with acute gastroenteritis.
A total of 82 fecal specimens which were known to be negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus and which were collected from infants and children with acute gastroenteritis in Chiang Mai, Thailand, from January to December 2005 were screened for human parechovirus (HPeV). HPeV was detected by reverse transcription-PCR with a primer pair that amplified the 5' untranslated region of its genome and was genotyped by sequencing of the VP1 region. HPeV was detected in 12 of 82 specimens tested, and the detection rate was found to be 14.6%. The capsid VP1 gene was successfully sequenced from nine of the HPeV strains detected. The HPeV strains studied clustered into four different genotypes, HPeV genotype 1 (HPeV1) to HPeV4, and the majority of the strains studied (five strains) belonged to HPeV1. This is the first finding of HPeV from children with acute gastroenteritis in Thailand. In addition, the diversity of the Thai HPeV strains was also noted.
Pham
Ngan Thi Kim
NT
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Trinh
Quang Duy
QD
Khamrin
Pattara
P
Maneekarn
Niwat
N
Shimizu
Hideaki
H
Okitsu
Shoko
S
Mizuguchi
Masashi
M
Ushijima
Hiroshi
H
D020121:5' Untranslated Regions; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000821:virology*; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013785:Thailand
D020121:5' Untranslated Regions; D036022:Capsid Proteins; D017931:DNA Primers