Monocyte-derived interleukin-10 depresses the Bordetella pertussis- specific gamma interferon response in vaccinated infants.
Antigen-specific gamma interferon (IFN-gamma) has been demonstrated to participate in protection against Bordetella pertussis infection. Circulating mononuclear cells from B. pertussis-infected and from pertussis-vaccinated infants secrete high amounts of IFN-gamma after in vitro stimulation by B. pertussis antigens, but with a large variation in the secreted IFN-gamma levels between individuals. We show here that the inhibition of the specific IFN-gamma response can be at least partially attributed to IL-10 secretion by monocytes. This IL-10 secretion was not associated with polymorphisms at positions -1082, -819, and -592 of the IL-10 gene promoter, suggesting that other genetic or environmental factors affect IL-10 expression and secretion.
Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles, Brussels, Belgium.
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Dirix
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Verscheure
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Goetghebuer
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Hainaut
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Debrie
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Locht
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Mascart
D018829:Adhesins, Bacterial / Q000494:pharmacology; D000483:Alleles; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001886:Bordetella pertussis / Q000276:immunology*; D005838:Genotype; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007223:Infant; D007371:Interferon-gamma / Q000819:agonists / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D018664:Interleukin-12 / Q000819:agonists / Q000096:biosynthesis / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008137:Longitudinal Studies; D037342:Pertussis Toxin / Q000494:pharmacology; D010567:Pertussis Vaccine / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014917:Whooping Cough / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control
D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; D007155:Immunologic Factors; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma; D037342:Pertussis Toxin