Effects of neurotrophic factors on growth and glial cell alignment of cultured adult spiral ganglion cells.
Adult spiral ganglion cells were cultured in chorus to assess the influence of the neurotrophins brain-derived neurotrophic factor, neurotrophin 3 and glial cell line-derived neurotrophic factor (GDNF) on neurite growth and Schwann cell alignment. Over 1500 measurements were collected using each factor at 10 ng/ml and all three in combination. Evaluation was made with GDNF at concentrations of up to 100 ng/ml. Neurite dimensions were assessed at days 5, 7, 9 and 11 using a computer-based program (Axon Analyzer). GDNF had a strong effect on spiral ganglion cell growth almost attaining the level of all three factors in combination. GDNF increased glial cell alignment and nerve bundle formation. Results show the potential of GDNF to maintain and possibly restore auditory nerve integrity.
Department of Otolaryngology, Head and Neck Surgery, Uppsala University Hospital, Uppsala, Sweden.
Marja
M
Boström
Shaden
S
Khalifa
Henrik
H
Boström
Wei
W
Liu
Ulla
U
Friberg
Helge
H
Rask-Andersen
D000328:Adult; D000818:Animals; D018929:Cell Culture Techniques; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D020933:Neurotrophin 3 / Q000494:pharmacology; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013136:Spiral Ganglion / Q000166:cytology* / Q000187:drug effects
D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020933:Neurotrophin 3