Clinicopathological significance of Sip1-associated epithelial mesenchymal transition in non-small cell lung cancer progression.
BACKGROUND
Epithelial-mesenchymal transition (EMT) is a key event in cancer progression. The expression of EMT-related factors in non-small cell lung cancer (NSCLC) and their impact on clinicopathological variables was examined.


METHODS
A total of 137 NSCLC patients who underwent surgical resections were investigated. The expression of Twist, Snail, smad-interacting protein 1 (Sip1), E-cadherin, N-cadherin, vimentin and beta-catenin was detected by immunohistochemical analyses.


RESULTS
The expression of Sip1 was associated with the reduced expression of E-cadherin (p=0.04) and the positive expression of N-cadherin (p=0.04). There was no association between the expression of Twist nor Snail and the epithelial or mesenchymal markers. The expression of Sip1 correlated with advanced T status (p=0.01), tumor diameter (p=0.01) and advanced stage (p=0.01). Furthermore, the expression of Sip1 was associated with poor postoperative overall survival (p=0.02).


CONCLUSIONS
The expression of Sip1 is significantly associated with tumor growth and poor prognoses in NSCLC and EMT might be activated via Sip1 expression and result in accelerated tumor growth and poor survival in NSCLC.
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Naoko
N
Miura
Tokujiro
T
Yano
Fumihiro
F
Shoji
Daigo
D
Kawano
Tomoyoshi
T
Takenaka
Kensaku
K
Ito
Yosuke
Y
Morodomi
Ichiro
I
Yoshino
Yoshihiko
Y
Maehara
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008648:Mesoderm / Q000473:pathology; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D051879:Twist-Related Protein 1 / Q000096:biosynthesis / Q000235:genetics; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics; D051176:beta Catenin / Q000096:biosynthesis / Q000235:genetics
D015703:Antigens, CD; C050361:CDH2 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; C108381:GEMIN2 protein, human; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D000071250:Snail Family Transcription Factors; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D014746:Vimentin; D051176:beta Catenin