Genetic fusions of heat-labile (LT) and heat-stable (ST) toxoids of porcine enterotoxigenic Escherichia coli elicit neutralizing anti-LT and anti-STa antibodies.
Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of diarrheal disease in humans and farm animals. E. coli fimbriae, or colonization factor antigens (CFAs), and enterotoxins, including heat-labile enterotoxins (LT) and heat-stable enterotoxins (ST), are the key virulence factors in ETEC diarrhea. Unlike fimbriae or LT, STa has not often been included as an antigen in development of vaccines against ETEC diarrhea because of its poor immunogenicity. STa becomes immunogenic only after being coupled with a strongly immunogenic carrier protein. However, native or shorter STa antigens either had to retain toxic activity in order to become antigenic or elicited anti-STa antibodies that were not sufficiently protective. In this study, we genetically mutated the porcine LT (pLT) gene for a pLT(192(R-->G)) toxoid and the porcine STa (pSTa) gene for three full-length pSTa toxoids [STa(11(N-->K)), STa(12(P-->F)), and STa(13(A-->Q))] and used the full-length pLT(192) as an adjuvant to carry the pSTa toxoid for pLT(192):pSTa-toxoid fusion antigens. Rabbits immunized with pLT(192):pSTa(12) or pLT(192):pSTa(13) fusion protein developed high titers of anti-LT and anti-STa antibodies. Furthermore, rabbit antiserum and antifecal antibodies were able to neutralize purified cholera toxin (CT) and STa toxin. In addition, preliminary data suggested that suckling piglets born by a sow immunized with the pLT(192):pSTa(13) fusion antigen were protected when challenged with an STa-positive ETEC strain. This study demonstrated that pSTa toxoids are antigenic when fused with a pLT toxoid and that the elicited anti-LT and anti-STa antibodies were protective. This fusion strategy could provide instructive information to develop effective toxoid vaccines against ETEC-associated diarrhea in animals and humans.
Zhang
Weiping
W
Center for Infectious Disease Research & Vaccinology/Veterinary Science Department, Box 2157, South Dakota State University, Brookings, SD 57006, USA. weiping.zhang@sdstate.edu
Zhang
Chengxian
C
Francis
David H
DH
Fang
Ying
Y
Knudsen
David
D
Nataro
James P
JP
Robertson
Donald C
DC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D057134:Antibodies, Neutralizing / Q000097:blood*; D001427:Bacterial Toxins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000276:immunology; D006152:Cyclic GMP; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000378:metabolism*; D004768:Enterotoxins / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D011817:Rabbits; D011994:Recombinant Proteins; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli; D006152:Cyclic GMP