Functional variants of glucokinase regulatory protein and apolipoprotein A5 genes in ischemic stroke.
Both the natural variants of the apolipoprotein A5 (APOA5) and the glucokinase regulatory protein gene (GCKR) have been shown to associate with increased fasting triglyceride levels. Here, we investigated the possible association of the functional variants of these two genes with non-fasting triglyceride levels and their susceptibility nature in ischemic stroke. A total of 513 stroke patients and 172 healthy controls were genotyped. All the APOA5 variants (T-1131C, IVS3 + G476A, C56G, and T1259C) were associated with increased triglyceride levels in all stroke patients and controls; except for T1259C, they all conferred risk for the disease. No such association was found for the examined GCKR rs1260326 (C1337T) variant. Furthermore, we examined the effects of specific combinations of the GCKR rs1260326 and APOA5 polymorphisms. Our findings confirmed the previous results regarding the association of APOA5 variants with triglyceride-level increase and stroke susceptibility of these alleles. By contrast, we could not detect any association of the studied GCKR allele with triglyceride levels or with the susceptibility of stroke in the same cohort of patients. In addition, the effect of APOA5 did not change significantly when specific combinations of the two genes were present.
Department of Medical Genetics, University of Pécs, Szigeti út 12, 7624, Pécs, Hungary.
Luca
L
Járomi
Veronika
V
Csöngei
Noémi
N
Polgár
Zoltán
Z
Szolnoki
Anita
A
Maász
Katalin
K
Horvatovich
Bernadett
B
Faragó
Csilla
C
Sipeky
Eniko
E
Sáfrány
Lili
L
Magyari
Péter
P
Kisfali
Márton
M
Mohás
Ingrid
I
Janicsek
Lilla
L
Lakner
Béla
B
Melegh
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000368:Aged; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics* / Q000378:metabolism; D002545:Brain Ischemia / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020521:Stroke / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D014280:Triglycerides / Q000097:blood
C437704:APOA5 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; C485100:GCKR protein, human; D020033:Protein Isoforms; D014280:Triglycerides