A micropatterned cell array with an integrated oxygen-sensitive fluorescent membrane.
We propose a simple method for producing micropatterned cell spots by photocatalytic lithography on a Pt porphyrin-based oxygen-sensitive polystyrene membrane that enables real-time imaging of oxygen consumption of patterned cell spots with sub-millimetre resolution.
Montagne
Kevin
K
LIMMS/CNRS-IIS, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. montagne@iis.u-tokyo.ac.jp
Komori
Kikuo
K
Yang
Fei
F
Tatsuma
Tetsu
T
Fujii
Teruo
T
Sakai
Yasuyuki
Y
D019069:Cell Respiration / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D010100:Oxygen / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D012966:Sodium Cyanide / Q000494:pharmacology; D046888:Tissue Array Analysis / Q000379:methods*
D008567:Membranes, Artificial; D011137:Polystyrenes; D011166:Porphyrins; D012966:Sodium Cyanide; D010100:Oxygen