Controlled gating of lysenin pores.
Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, USA. dfologea@uark.edu
Daniel
D
Fologea
Eric
E
Krueger
Rachel
R
Lee
Matt
M
Naglak
Yuriy
Y
Mazur
Ralph
R
Henry
Greg
G
Salamo
D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000737:chemistry*; D013109:Sphingomyelins; D014118:Toxins, Biological / Q000737:chemistry*
D008051:Lipid Bilayers; D013109:Sphingomyelins; D014118:Toxins, Biological; C107040:lysenin