The tissue distribution in mice and pharmacokinetics in rabbits of oxaliplatin liposome.
A rapid, sensitive, and simple high-performance liquid chromatographic (HPLC) method with an ultraviolet detector (UV) has been developed for the determination of oxaliplatin in the plasma of rabbits and tissues of mice. The sample preparation was carried out by complexation with 0.5 mL of DETC (diethyl-dithiocarbamate) solution and extracted by ether and chloroform. Then, 20 microL of supernatant was injected into the HPLC system with 0.25 mol/L of sodium chloride solution and methanol (30:70 v/v) as the mobile phase at a flow rate of 1.0 mL/min. Separation was performed with a C(18) column at 25 degrees C. The peak was detected at 254 nm. The calibration curve was linear (R(2) > or = 0.9995) in the concentration range of 0.1 approximately 200 microg/mL in plasma and tissues. The intra- and interday variation coefficients were not more than 2.61 and 3.83%, respectively. The limit of detection was 20 ng/mL. The mean recoveries of oxaliplatin were ranged from 97.83 to 104.17% in plasma and tissues. The present method has been successfully applied to the pharmacokinetic study of oxaliplatin liposome in mice and rabbits.
School of Chemical Engineering, Wuhan University of Technology, Wuhan, People's Republic of China. pharmlxp@163.com
Xiao-ping
XP
Liu
Dan-qing
DQ
Geng
Hai-xing
HX
Xu
Xiao-hui
XH
Sui
D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D008081:Liposomes; D051379:Mice; D009944:Organoplatinum Compounds / Q000493:pharmacokinetics*; D000077150:Oxaliplatin; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution
D000970:Antineoplastic Agents; D008081:Liposomes; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin