Selection for increased nitric oxide production does not increase resistance to Marek's disease in a primary broiler breeder line.
Two primary broiler breeder lines, A and B, were examined for their potential to produce nitric oxide (NO) after stimulating splenocytes from 20-day-old embryos with lipopolysaccharide and interferon-gamma. Significant differences were found between lines A and B. Overall, line A had a higher response than line B, but line A also had a large degree of variation between individual sire families. Selection for high and low responders within line A resulted in the segregation of high- and low-responder sire families. Offspring from sire families selected for high and low NO responses and from a nonselected control group from line A were challenged with RB-1B Marek's disease (MD) virus to determine whether these differences could be used to select for improved resistance to MD. Virus isolation rates at 6 and 10 days postinfection were not significantly different, but unexpectedly, the MD incidence in the high-responder group was significantly higher than in the other two groups.
Buscaglia
Celina
C
Cátedra de Zootecnia Especial III (Aves y Piliferos), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296 (B190AVW) La Plata, Argentina.
O'Connell
Priscilla H
PH
Jarosinski
Keith W
KW
Pevzner
Igal
I
Schat
Karel A
KA
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005260:Female; D020022:Genetic Predisposition to Disease; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008380:Marek Disease / Q000235:genetics* / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D012641:Selection, Genetic; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014766:Viremia / Q000235:genetics
D008070:Lipopolysaccharides; D009569:Nitric Oxide; D007371:Interferon-gamma