Genetic characterization of G3 rotaviruses detected in Italian children in the years 1993-2005.
In recent years an apparent increase in the frequency of detection of G3P[8] rotaviruses has been observed worldwide. Similarly, in Italy G3P[8] strains have been detected sporadically and in a scattered fashion over 20 years, whereas in 2003 and 2005 G3P[8] rotavirus activity increased markedly. By analysis of the VP7, VP4, VP6, and NSP4 genes of a selection of G3P[8] rotaviruses detected between 1993 and 2005, a remarkable sequence conservation was observed in the VP7, VP4, and VP6 genes. By converse, after 2002 the Italian G3P[8] strains were found to possess unique mutations in significant regions of the NSP4 protein.
Dipartimento di Scienze per la Promozione della Salute G. D'Alessandro, Sezione di Microbiologia, Università di Palermo, Palermo, Italy.
Simona
S
De Grazia
Vito
V
Martella
Claudia
C
Colomba
Antonio
A
Cascio
Serenella
S
Arista
Giovanni M
GM
Giammanco
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D036022:Capsid Proteins / Q000032:analysis / Q000235:genetics; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D017423:Sequence Analysis, RNA; D014118:Toxins, Biological / Q000032:analysis / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000235:genetics
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D012367:RNA, Viral; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins