A bombesin-like peptide from skin of Sanguirana varians.
Bombesin-like peptides (BLPs) are a family of neuro-endocrinic peptides that mediates a variety of biological activities. A BLP named bombesin-SV from the skin secretions of the frog, Sanguirana varians, was purified and characterized. Its amino acid sequence is pEMIFGAPMWALGHLM-NH2 determined by Edman degradation and mass spectrometry. The cDNA encoding bombesin-SV precursor was cloned from skin cDNA library of S. varians. The precursor is composed of 67 amino acid residues including a copy of mature bombesin-SV. Two predicted enzymatic processing sites (-Lys-Arg-) are flanked at the mature bombesin-SV sequence. In the in vitro myotropic contraction experiment, the synthesized bombesin-SV displayed the stimulating effect toward rat stomach strips, suggesting that bombesin-SV acts as agonist as most other BLPs do.
Department of Gastroenterology, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China. myldu@sina.com
Yinglei
Y
Miao
Wenliang
W
Li
Liping
L
Duan
Yuliang
Y
Xiao
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001839:Bombesin / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D011898:Ranidae; D051381:Rats; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D013270:Stomach / Q000502:physiology
D018076:DNA, Complementary; D010455:Peptides; D001839:Bombesin