A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests.
BACKGROUND
Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method.


METHODS
The susceptibility of two bacterial and two fungal strains to a 6h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods.


RESULTS
Three rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones.


CONCLUSIONS
The development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing.
Lafond
Mickael
M
UPCAM iSm2, Case 342, Université Paul Cézanne, Av. Escadrille Normandie Niémen, 13397 Marseille cedex 13, France.
Vidal
Nicolas
N
Letourneux
Yves
Y
Brunel
Jean Michel
JM
D000263:Adenylate Kinase / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D049408:Luminescent Agents / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D049408:Luminescent Agents; D016660:NAD(P)H Dehydrogenase (Quinone); D000263:Adenylate Kinase