[Use of PCR for TB bacteria on BAL in early diagnosis of tubercular lobitis].
In June 2008 a 23-year-old immunocompetent came to our observation, without fever and with an occasional cough for 2 months, who showed two chest X-rays and a CT, performed respectively 60, 40 and 20 days earlier, that pointed to a small lobitis at the right lung base. The patient had already undergone several antibiotic therapies that had not changed the X-graphic framework. On presentation, routine blood tests and cultural examinations of sputum were carried out to detect common germs, fungi and TB bacteria (microscopic observation, cultivation and PCR), and a new antibiotic therapy (piperacillin/tazobactam) was started. Since the radiological picture appeared unchanged after 10 days of therapy and the examinations (microscopic observation and PCR) were negative, bronchoscopy with bacteriological evaluation of BAL was performed, which was positive to Mycobacterium tuberculosis, and then tubercular lobitis was diagnosed. Therefore a specific therapy - rifampin (RMP), isoniazid (INH), etambutol (EMB), pyrazinamid (PZA) - was started and changed after 10 days due to the growth of mycobacteria resistant to INH and EMB on examination of sputum. Consequently, the early use of PCR on BAL allows, in skilled hands, small aspecific lobitis to be diagnosed more rapidly than using cultural examination of sputum.
Franco
Alfredo
A
VIII Divisione di Malattie Infettive ad Alta Complessità, A.O.R.N. Cotugno, Napoli, Italy.
Aprea
Lucia
L
Conte
Marco
M
Manzillo
Elio
E
Marocco
Alessandro
A
Martucci
Fiorella
F
Parrella
Roberto
R
Pizzella
Teresa
T
Simioli
Francesco
F
Izzo
Crescenzo Maria
CM
D000995:Antitubercular Agents / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D001999:Bronchoscopy; D004269:DNA, Bacterial / Q000032:analysis*; D042241:Early Diagnosis; D006801:Humans; D007121:Immunocompetence; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology; D014057:Tomography, X-Ray Computed; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000382:microbiology; D055815:Young Adult
D000995:Antitubercular Agents; D004269:DNA, Bacterial