Adaptive harmonic generation microscopy of mammalian embryos.
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.
1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
Alexander
A
Jesacher
Anisha
A
Thayil
Kate
K
Grieve
Delphine
D
Débarre
Tomoko
T
Watanabe
Tony
T
Wilson
Shankar
S
Srinivas
Martin
M
Booth
D000388:Air; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods; D004622:Embryo, Mammalian / Q000378:metabolism*; D004867:Equipment Design; D005898:Glass; D007091:Image Processing, Computer-Assisted; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D012031:Refractometry / Q000379:methods; D013997:Time Factors