CC chemokine receptor-1 activates intimal smooth muscle-like cells in graft arterial disease.
BACKGROUND
Graft arterial disease (GAD) limits long-term solid-organ allograft survival. The thickened intima in GAD contains smooth muscle-like cells (SMLCs), leukocytes, and extracellular matrix. The intimal SMLCs in mouse GAD lesions differ from medial smooth muscle cells in their function and phenotype. Although intimal SMLCs may originate by migration and modulation of donor medial cells or by recruitment of host-derived precursors, the mechanisms that underlie their localization within grafts and the factors that drive these processes remain unclear.


RESULTS
This study of aortic transplantation in mice demonstrated an important function for chemokines beyond their traditional role in leukocyte recruitment and activation. Intimal SMLCs, but not medial smooth muscle cells, express functional CC chemokine receptor-1 (CCR1) and respond to RANTES by increased migration and proliferation. Although RANTES infusion in vivo promoted inflammatory cell accumulation in the adventitia of aortic allografts of wild-type and CCR1-deficient recipients, it increased GAD intimal thickening with SMLC proliferation in only the wild-type hosts. Aortic allografts transplanted into CCR1-deficient mice after wild-type bone marrow transplantation did not develop intimal lesions, which indicates that CCR1-bearing inflammatory cells do not contribute to intimal lesion formation. Moreover, RANTES induced SMLC proliferation in vitro but did not promote medial smooth muscle cell growth. Blockade of CCR5 attenuated RANTES-induced T-cell and monocyte/macrophage proliferation but did not affect RANTES-induced SMLC proliferation, consistent with a larger role of CCR1-binding chemokines in SMLC migration and proliferation and GAD development.


CONCLUSIONS
These studies provide a novel mechanistic insight into the formation of vascular intimal hyperplasia and suggest a novel therapeutic strategy for preventing allograft arteriopathy.
Donald W. Reynolds Cardiovascular Clinical Research Center, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. ksmz@rics.bwh.harvard.edu
Koichi
K
Shimizu
Manabu
M
Minami
Rica
R
Shubiki
Marco
M
Lopez-Ilasaca
Lindsey
L
MacFarlane
Yukiko
Y
Asami
Yuxin
Y
Li
Richard N
RN
Mitchell
Peter
P
Libby
D000818:Animals; D001011:Aorta / Q000637:transplantation*; D001018:Aortic Diseases / Q000209:etiology*; D049109:Cell Proliferation; D018946:Chemokine CCL5 / Q000502:physiology; D006965:Hyperplasia; D051379:Mice; D018345:Mice, Knockout; D032389:Myocytes, Smooth Muscle / Q000473:pathology*; D054389:Receptors, CCR1 / Q000502:physiology*; D019737:Transplants; D017539:Tunica Intima / Q000473:pathology*
D018946:Chemokine CCL5; D054389:Receptors, CCR1