Cytotoxic activity and G1 cell cycle arrest of a Dienynone from Echinacea pallida.
In the present study, a further investigation of the cytotoxic activity of an acetylenic constituent of Echinacea pallida roots, namely, pentadeca-(8 Z,13 Z)-dien-11-yn-2-one, was performed, revealing a concentration-dependent cytotoxicity on several human cancer cell lines, including leukemia (Jurkat and HL-60), breast carcinoma (MCF-7), and melanoma (MeWo) cells. As part of its mechanism of action, the ability of this constituent to arrest the cell cycle in the G1 phase was demonstrated on HL-60 cells. Furthermore, a stability test of the target compound over 72 h was carried out, indicating that the cytotoxic activity can be attributed mainly to the genuine, not oxidized, molecule.
Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Pisa, Italy. andrea.chicca@ittumori.it
Andrea
A
Chicca
Barbara
B
Adinolfi
Federica
F
Pellati
Giulia
G
Orlandini
Stefania
S
Benvenuti
Paola
P
Nieri
D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D020900:Echinacea; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry
D000970:Antineoplastic Agents; D007659:Ketones; D010936:Plant Extracts; C549757:pentadeca-(8Z,13Z)-dien-11-yn-2-one