Effect of pre- and postnatal exposure to urban air pollution on myocardial lipid peroxidation levels in adult mice.
Exposure to air pollution can elicit cardiovascular health effects. Children and unborn fetuses appear to be particularly vulnerable. However, the mechanisms involved in cardiovascular damage are poorly understood. It has been suggested that the oxidative stress generated by air pollution exposure triggers tissue injury. To investigate whether prenatal exposure can enhance oxidative stress in myocardium of adult animals, mice were placed in a clean chamber (CC, filtered urban air) and in a polluted chamber (PC, São Paulo city) during the gestational period and/or for 3 mo after birth, according to 4 protocols: control group-prenatal and postnatal life in CC; prenatal group-prenatal in PC and postnatal life in CC; postnatal group-prenatal in CC and postnatal life in PC; and pre-post group-prenatal and postnatal life in PC. As an indicator of oxidative stress, levels of lipid peroxidation in hearts were measured by malondialdehyde (MDA) quantification and by quantification of the myocardial immunoreactivity for 15-F2t-isoprostane. Ultrastructural studies were performed to detect cellular alterations related to oxidative stress. Concentration of MDA was significantly increased in postnatal (2.45 +/- 0.84 nmol/mg) and pre-post groups (3.84 +/- 1.39 nmol/mg) compared to the control group (0.31 +/- 0.10 nmol/mg) (p < .01). MDA values in the pre-post group were significantly increased compared to the prenatal group (0.71 +/- 0.15 nmol/mg) (p = .017). Myocardial isoprostane area fraction in the pre-post group was increased compared to other groups (p < or = .01). Results show that ambient levels of air pollution elicit cardiac oxidative stress in adult mice, and that gestational exposure may enhance this effect.
Department of Pathology, University of São Paulo, Brazil. nilsa@usp.br
Nilsa Regina
NR
Damaceno-Rodrigues
Mariana Matera
MM
Veras
Elnara Márcia
EM
Negri
Ana Claudia Tedesco
AC
Zanchi
Claudia Ramos
CR
Rhoden
Paulo Hilário Nascimento
PH
Saldiva
Marisa
M
Dolhnikoff
Elia Garcia
EG
Caldini
D000375:Aging / Q000187:drug effects / Q000473:pathology; D000397:Air Pollution / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D019570:Inhalation Exposure / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism* / Q000473:pathology*; D014504:Urban Health