Scale-up of diluted sulfuric acid hydrolysis for producing sugarcane bagasse hemicellulosic hydrolysate (SBHH).
Sugarcane bagasse was pretreated with diluted sulfuric acid to obtain sugarcane bagasse hemicellulosic hydrolysate (SBHH). Experiments were conducted in laboratory and semi-pilot reactors to optimize the xylose recovery and to reduce the generation of sugar degradation products, as furfural and 5-hydroxymethylfurfural (HMF). The hydrolysis scale-up procedure was based on the H-Factor, that combines temperature and residence time and employs the Arrhenius equation to model the sulfuric acid concentration (100 mg(acid)/g(dm)) and activation energy (109 kJ/mol). This procedure allowed the mathematical estimation of the results through simulation of the conditions prevailing in the reactors with different designs. The SBHH obtained from different reactors but under the same H-Factor of 5.45+/-0.15 reached similar xylose yield (approximately 74%) and low concentration of sugar degradation products, as furfural (0.082 g/L) and HMF (0.0071 g/L). Also, the highest lignin degradation products (phenolic compounds) were rho-coumarilic acid (0.15 g/L) followed by ferulic acid (0.12 g/L) and gallic acid (0.035 g/L). The highest concentration of ions referred to S (3433.6 mg/L), Fe (554.4 mg/L), K (103.9 mg/L). The H-Factor could be used without dramatically altering the xylose and HMF/furfural levels. Therefore, we could assume that H-Factor was directly useful in the scale-up of the hemicellulosic hydrolysate production.
Rodrigues
Rita de Cássia L B
Rde C
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, P.O. Box 116, 12600-970 Lorena, SP, Brazil. rita@debiq.eel.usp.br
Rocha
George J M
GJ
Rodrigues
Durval
D
Filho
Hélcio J I
HJ
Felipe
Maria das Graças A
Md
Pessoa
Adalberto
A
D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000138:chemical synthesis* / Q000737:chemistry / Q000648:ultrastructure; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D010865:Pilot Projects; D011134:Polysaccharides / Q000138:chemical synthesis*; D031786:Saccharum / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014994:Xylose / Q000032:analysis
D011134:Polysaccharides; D013464:Sulfuric Acids; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; D014994:Xylose; C033158:sulfuric acid