[Application of magnetic particle antibody immunoassay in detection of anti-Schistosoma japonicum egg antibody].
OBJECTIVE
To establish a magnetic particle antibody immunoassay (MPAIA) for the detection of specific antibody in sera of schistosomiasis patients.


METHODS
Fluorescein isothiocyanate (FITC) was used to label Schistosoma japonicum soluble egg antigen (Sj-SEA). Anti-human IgG coated with alkaline phosphatase (ALP) as enzyme-labeled second antibody, and magnetic beads were coupled with sheep anti-FITC antibody as solid phase. Phenolphthale in monophosphate was used as substrate to set up MPAIA for the detection. Serum samples from cases with schistosomiasis or other helminth infections were tested.


RESULTS
The positive rate of MPAIA was 96.7% (116/120) with the sera of S. japonicum-infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases. The positive titer with reference sample was 1: 1,600. The precision was lower than 10%. The MPAIA tips can be stored at 4 degrees C for 12 months.


CONCLUSIONS
MPAIA shows a high sensitivity, proper specificity and long-term validity for schistosomiasis detection.
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Jin
J
Huang
Zhen-Shi
ZS
Liu
Lan
L
Yao
Su-Juan
SJ
Dong
Qi-Lin
QL
He
Tao
T
Xiong
Lan-Ying
LY
Wei
Zheng-Ming
ZM
Eang
Chang-Fu
CF
Jiang
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008280:Magnetics; D010270:Parasite Egg Count / Q000379:methods*; D012549:Schistosoma japonicum / Q000276:immunology*; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth