The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex.
The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in severe defects in biorientation and organization of the spindle midzone and midbody. Our results indicate that a major function of the Nup107 complex in mitosis is to ensure the proper localization of the CPC at the centromere.
Platani
Melpomeni
M
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH93JR, United Kingdom. m.platani@ed.ac.uk
Santarella-Mellwig
Rachel
R
Posch
Markus
M
Walczak
Rudolf
R
Swedlow
Jason R
JR
Mattaj
Iain W
IW
D000705:Anaphase / Q000187:drug effects; D064107:Aurora Kinase B; D064127:Aurora Kinases; D020090:Chromosome Segregation / Q000187:drug effects; D002877:Chromosomes, Human / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D048749:Cytokinesis / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D018386:Kinetochores / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013379:Substrate Specificity / Q000187:drug effects; D013871:Thiones / Q000378:metabolism
C091489:KIF2C protein, human; C512772:NUP107 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011743:Pyrimidines; D013871:Thiones; C400223:monastrol; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D016547:Kinesins; D017239:Paclitaxel; D015739:Nocodazole