Profile and removal of endocrine disrupting chemicals by using an ER/AR competitive ligand binding assay and chemical analyses.
An estrogen receptor (ER)/androgen receptor (AR) ligand competitive binding assay (ER/AR-binding assay) and chemical analyses were used to evaluate the endocrine disrupting chemicals (EDCs) behavior of two municipal wastewater treatment plants (WWTPs) (K and S). In the influents, estrone (E1), androsterone (A), androstenedione (AD), BPA (bisphenol A), NP (nonylphenol) and daidzein (DZ) were detected in high amounts with subsequent 24 h-average concentrations of 350, 1000, 29, 1300, 3900, and 5700 ng/L in K-WWTP and of 310, 620, 59, 1600, 2600, and 8400 ng/L in S-WWTP. The estrogenic (androgenic) activity as 17beta-estradiol (E2) equivalents (EEQ) or testosterone (Te) equivalents (TEQ) was consequently 620 ng E2/L (570 ng Te/L) and 580 ng E2/L (800 ng Te/L) for the two WWTPs. The removal efficiencies of the above mentioned sole target chemicals were 51%-100% for K-WWTP and 55.6%-100% for S-WWTP. The removal efficiencies of EEQ were about 73% for both WWTPs, while the removal efficiencies of TEQ were 62.1% for K-WWTP and 98.4% for S-WWTP. In addition, chemical-derived EEQ were about 1.2%-52.4% of those by ER-binding assay for K-WWTP and the corresponding ratios were 1.3%-83.3% for S-WWTP, while chemical derived TEQ were less than 3% of values measured by the AR-binding assay for both WWTPs.
Department of Urban Engineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. liuzehua@sues.edu.cn
Ze-hua
ZH
Liu
Mamoru
M
Ito
Yoshinori
Y
Kanjo
Atsushi
A
Yamamoto
D000735:Androstenedione / Q000302:isolation & purification / Q000378:metabolism; D000738:Androsterone / Q000302:isolation & purification / Q000378:metabolism; D001559:Benzhydryl Compounds; D001681:Biological Assay / Q000379:methods*; D052244:Endocrine Disruptors / Q000302:isolation & purification* / Q000378:metabolism; D004784:Environmental Monitoring; D004958:Estradiol / Q000302:isolation & purification / Q000378:metabolism; D004970:Estrone / Q000302:isolation & purification / Q000378:metabolism; D007529:Isoflavones / Q000302:isolation & purification / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011944:Receptors, Androgen / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D013739:Testosterone / Q000302:isolation & purification / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods*; D000098826:Bisphenol A Compounds
D000735:Androstenedione; D000738:Androsterone; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D004970:Estrone; D007529:Isoflavones; D010636:Phenols; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D013739:Testosterone; D014874:Water Pollutants, Chemical; C004742:daidzein; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds