An easy and reproducible model of kidney transplantation in rats.
OBJECTIVE
Kidney transplantation in rats is an important research model. Various methods have been reported, but there is no "standard operation." We investigated a 1-stage versus a 2-stage native nephrectomy and the type of ureteral anastomosis seeking to establish a standard, reproducible and successful method.


METHODS
We used PVG (RT1c-RT1Ac: B/Dc) male rats, weighing approximately 200 to 250 g, that underwent transplantation after right recipient nephrectomy. Left recipient nephrectomy was performed either 10 days later or simultaneously. The ureteric anastomosis was fashioned 2 ways: using a ureteral stent or by bladder insertion.


RESULTS
Urinary complications (obstruction or reflux) were observed in 77.8% when a ureteral stent was used for the ureteric anastomosis versus 28.6% when using the bladder insertion technique (P = .0211). Transplanted rats with nephrectomy of both native kidneys at the time of grafting showed a perioperative mortality of 70%, whereas those hosts with a 2-stage nephrectomy displayed a mortality rate of 22% (P = .0025).


CONCLUSIONS
The bladder insertion technique reduced the incidence of urological complications in rats. In addition, unilateral native nephrectomy at the time of operation with delayed contralateral nephrectomy was better tolerated than simultaneous bilateral nephrectomy. These 2 surgical variants allowed us to perform kidney transplantation with a high degree of success.
Department of Abdominal Transplant Surgery, University Hospitals Leuven, 3000 Leuven, Belgium.
T
T
Soma
E
E
Lerut
A
A
Billiau
M
M
Waer
J
J
Goebels
T
T
Koshiba
S
S
Uemoto
J
J
Pirenne
D000273:Adipose Tissue / Q000601:surgery; D000714:Anastomosis, Surgical; D000818:Animals; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D009392:Nephrectomy / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D015607:Stents; D014513:Ureter / Q000601:surgery; D001743:Urinary Bladder / Q000601:surgery