Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs.
Intercellular exchange of protein and RNA-containing microparticles is an increasingly important mode of cell-cell communication. Here we investigate if mesenchymal stem cells (MSCs) known for secreting therapeutic paracrine factors also secrete RNA-containing microparticles. We observed that human embryonic stem cell (hESC)-derived MSC conditioned medium contained small RNAs (less than 300 nt) encapsulated in cholesterol-rich phospholipid vesicles as evidenced by their RNase sensitivity only in the presence of a sodium dodecyl sulfate-based cell lysis buffer, phospholipase A2 and a chelator of cholesterol, cyclodextrin and the restoration of their lower than expected density by detergent or phospholipase A2 treatment. MicroRNAs (miRNAs) such as hsa-let-7b and hsa-let-7g were present in a high precursor (pre)- to mature miRNA ratio by microarray analysis and quantitative reverse transcription-polymerase chain reaction. The pre-miRNAs were cleaved to mature miRNA by RNase III in vitro. High performance liquid chromatography-purified RNA-containing vesicles have a hydrodynamic radius of 55-65 nm and were readily taken up by H9C2 cardiomyocytes. This study suggests that MSCs could facilitate miRNA-mediated intercellular communication by secreting microparticles enriched for pre-miRNA.
Chen
Tian Sheng
TS
Institute of Medical Biology, A*STAR, NUS, Singapore.
Lai
Ruenn Chai
RC
Lee
May May
MM
Choo
Andre Boon Hwa
AB
Lee
Chuen Neng
CN
Lim
Sai Kiang
SK
D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic
D035683:MicroRNAs; D010743:Phospholipids; D012322:RNA Precursors; D012259:Ribonuclease, Pancreatic