Emergence of SHV-2a extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a university hospital in Tunisia.
Extended-spectrum beta-lactamases (ESBLs) in Pseudomonas aeruginosa are increasingly reported worldwide. In our study, a total of 70 clinical isolates of multidrug-resistant P. aeruginosa were studied. Isoelectric focusing electrophoresis, PCR, and PCR product sequencing were designed to characterize the contained ESBLs. The Double Disk Synergy Test in Cloxacillin (250 microg/ml)-containing Mueller-Hinton agar plates with a 20 mm distance between disks was the most reliable ESBL-screening method. Seven out of 70 multidrug-resistant P. aeruginosa clinical isolates were positive for ESBL and have the bla(SHV-2a) ESBL gene. The bla(SHV-2a)-positive isolates were clonally related according to Enterobacterial Repetetive Intergenic Consensus-PCR (ERIC-PCR) results. The bla(SHV-2a) gene was found to be chromosomally located, and the flanking IS26 sequence in the immediate upstream region of the bla(SHV-2a) gene was detected in all SHV-2a-producing isolates. This is the first report of SHV-2a-producing P. aeruginosa isolates from Tunisia.
Mansour
Wejdene
W
Research Unit of Infections with Bacteria Resistant to Multiple Antibiotics (UR/29/04), Laboratory of Microbiology, Sousse, Tunisia. wejmbr@yahoo.fr
Dahmen
Safia
S
Poirel
Laurent
L
Charfi
Karama
K
Bettaieb
Dalia
D
Boujaafar
Noureddine
N
Bouallegue
Olfa
O
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003023:Cloxacillin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006785:Hospitals, University; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014416:Tunisia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; C490029:beta-lactamase SHV-2a; D001618:beta-Lactamases; D003023:Cloxacillin