Guanosine + OH radical reaction in aqueous solution: a reinterpretation of the UV-vis data based on thermodynamic and kinetic calculations.
Thermodynamic and kinetic calculations have been used to reinterpret the UV-vis data related to the OH radical oxidation of guanosine. The main channel of reaction (70-75%) is proposed to be the formation of a guanosine radical cation followed by deprotonation. It accounts for both the absorbance decay at approximately 620 nm and the build-up at approximately 300 nm. A secondary channel yielding the G8OH adduct was found to contribute to the overall reaction by 12% at least.
Galano
Annia
A
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, 09340 México D. F., México. agalano@prodigy.net.mx
Alvarez-Idaboy
J Raul
JR
D006151:Guanosine / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D012996:Solutions; D014867:Water; D006151:Guanosine; D017665:Hydroxyl Radical