Co-appearance of autoantibody-producing B220(low) B cells with NKT cells in the course of hepatic injury.
Severe hepatic injury is induced by Concanavalin A (Con A) administration in mice, the major effector cells being CD4(+) T cells, NKT cells and macrophages. Since autologous lymphocyte subsets are associated with tissue damage, Con A-induced hepatic injury is considered to be autoimmune hepatitis. However, it has remained to be investigated how autoantibodies and B-1 cells are responsible for this phenomenon. In this study, it was demonstrated that autoantibodies which were detected using Hep-2 cells in immunofluorescence tests and using double-strand (ds) DNA in the ELISA method, appeared after Con A administration (a peak at day 14). Moreover, autoantibody-producing B220(low) cells (i.e., B-1 cells) also appeared at this time. Purified B220(low) cells were found to have a potential to produce autoantibodies. These results suggest that Con A-induced hepatic injury indeed includes the mechanism of autoimmune hepatitis.
Department of Immunology, Niigata University School of Medicine, Niigata 951-8510, Japan.
Yuki
Y
Fujii
Hiroki
H
Kawamura
Toshihiko
T
Kawamura
Yasuhiro
Y
Kanda
Hiroaki
H
Matsumoto
Takahiro
T
Kobayashi
Takashi
T
Yamamoto
Takashi
T
Aoyama
Toru
T
Abo
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D045744:Cell Line, Tumor; D003208:Concanavalin A; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006520:Hepatitis, Animal / Q000097:blood / Q000139:chemically induced / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D018655:Lymphocyte Count; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000166:cytology / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors
D001323:Autoantibodies; D003208:Concanavalin A; D000410:Alanine Transaminase