Dexamethasone and the inflammatory response in explants of human omental adipose tissue.
Dexamethasone is a synthetic glucocorticoid that is a potent anti-inflammatory agent. The present studies examined the changes in gene expression of 64 proteins in human omental adipose tissue explants incubated for 48h both in the absence and presence of dexamethasone as well as the release of 8 of these proteins that are putative adipokines. The proteins were chosen because they are inflammatory response proteins in other cells, are key regulatory proteins or are proteins with known functions. About 50% were significantly up-regulated while about 10% were unchanged and the remaining 40% were down-regulated. Dexamethasone significantly up-regulated the expression of about 33% of the proteins but down-regulated the expression of about 12% of the proteins. We conclude that dexamethasone is a selective anti-inflammatory agent since it inhibits only about one-fourth of the proteins up-regulated during in vitro incubation of human omental adipose tissue.
Fain
John N
JN
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA. jfain@uthsc.edu
Cheema
Paramjeet
P
Madan
Atul K
AK
Tichansky
David S
DS
D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D009852:Omentum / Q000033:anatomy & histology / Q000187:drug effects / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology; D046509:Tissue Culture Techniques; D014475:Uncoupling Agents / Q000494:pharmacology; D015854:Up-Regulation
D000893:Anti-Inflammatory Agents; D016209:Interleukin-8; D012333:RNA, Messenger; D014475:Uncoupling Agents; D012402:Rotenone; D003907:Dexamethasone