Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction.
Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.
Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Thailand. pewpan@kku.ac.th
Pewpan M
PM
Intapan
Tongjit
T
Thanchomnang
Viraphong
V
Lulitanond
Phunthira
P
Pongsaskulchoti
Wanchai
W
Maleewong
D000818:Animals; D005243:Feces / Q000469:parasitology*; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008297:Male; D009891:Opisthorchis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity