Ammonium and phosphate limitation in 1,3-propanediol production by Klebsiella pneumoniae.
Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia-limited fed-batch cultures produced 61 g 1,3-PD l(-1) and a total of 15 g l(-1) organic acid in 36 h. However, phosphate-limited fed-batch cultures excreted 61 g lactate l(-1) and 44 g 1,3-PD l(-1).
National Engineering Laboratory for Biomass Power Generation Equipment, School of Renewable Energy, North China Electric Power University, Beijing, 102206, China, zhengzm@ncepu.edu.cn.
Zong-Ming
ZM
Zheng
Yun-Zhen
YZ
Xu
Ti-Peng
TP
Wang
Chang-Qing
CQ
Dong
Yong-Ping
YP
Yang
De-Hua
DH
Liu
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D003198:Computer Simulation; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008954:Models, Biological; D010710:Phosphates / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*
D010710:Phosphates; D011409:Propylene Glycols; D000644:Quaternary Ammonium Compounds; C041787:1,3-propanediol