Significant CD5 expression on normal stage 3 hematogones and mature B Lymphocytes in bone marrow.
B-cell maturation from hematogones to mature B cells in bone marrow exhibits a consistent, complex spectrum of sequential antigen expression. CD5 expression, however, has not been characterized. We studied the dynamics of CD5 expression on developing B cells by 4-color flow cytometry in 32 patients, aged 9 months to 63 years, with hematogone hyperplasia (>3.5% of total events). The mean percentage of hematogones was 8.1%. We demonstrate consistent CD5 expression on normal, polytypic B cells in a continuum, predominantly at later stages of maturation, specifically on stage 3 hematogones and mature B cells. Awareness of this normal pattern of CD5 expression on B-cell subsets has implications in the analysis of minimal residual disease of CD5+ B-lineage non-Hodgkin lymphomas.
Fuda
Franklin S
FS
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USA.
Karandikar
Nitin J
NJ
Chen
Weina
W
D000328:Adult; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D018956:CD5 Antigens / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007223:Infant; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000378:metabolism / Q000473:pathology
D018956:CD5 Antigens