A chemical platform for improved induction of human iPSCs.
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA.
Tongxiang
T
Lin
Rajesh
R
Ambasudhan
Xu
X
Yuan
Wenlin
W
Li
Simon
S
Hilcove
Ramzey
R
Abujarour
Xiangyi
X
Lin
Heung Sik
HS
Hahm
Ergeng
E
Hao
Alberto
A
Hayek
Sheng
S
Ding
D001549:Benzamides / Q000494:pharmacology; D002454:Cell Differentiation / Q000235:genetics*; D004149:Dioxoles / Q000494:pharmacology; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D048369:MAP Kinase Kinase 1 / Q000037:antagonists & inhibitors; D011743:Pyrimidines / Q000494:pharmacology; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D013844:Thiazoles / Q000494:pharmacology; D014161:Transduction, Genetic
C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D001549:Benzamides; D004149:Dioxoles; D011743:Pyrimidines; D018125:Receptors, Transforming Growth Factor beta; D013844:Thiazoles; C545214:thiazovivin; C506614:mirdametinib; D004159:Diphenylamine; D048369:MAP Kinase Kinase 1