[Molecular cloning and characterization of calcineurin A in Setosphaeria turcica].
A Setosphaeria turcica gene encoding the catalytic subunit of calcineurin was cloned using degenerated primers corresponding to conserved domains of Ser/Thr protein phosphatases and its complete cDNA (GenBank accession No. EF 407562) was obtained with RACE method. It's validated single copied model by southern hybridization. Furthermore, the CNA inhibitor Cyclosporin A (CsA) exhibited potent antifungal activity against conidial germination and appressorium formation of S. turcica. The inhibition ratio was positively correlated to CsA concentration. However, appressorium formation was more sensitive than conidium germination to the inhibitor at the same concentration. It was suggested that CNA might play an important role in the pathogenicity of S. turcica.
Molecular Plant Pathology Laboratory of Agricultural University of Hebei, Baoding 071001, China. lizhiyongds@126.com
Zhi-Yong
ZY
Li
Zhi-Min
ZM
Hao
Zhi-Ping
ZP
Dong
He-Long
HL
Si
Jin-Gao
JG
Dong
D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D019703:Calcineurin / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny
D019703:Calcineurin