Determination of the core promoter regions of the Saccharomyces cerevisiae RPS3 gene.
Ribosomal protein genes (RPG), which are scattered throughout the genomes of all eukaryotes, are subjected to coordinated expression. In yeast, the expression of RPGs is highly regulated, mainly at the transcriptional level. Recent research has found that many ribosomal proteins (RPs) function in multiple processes in addition to protein synthesis. Therefore, detailed knowledge of promoter architecture as well as gene regulation is important in understanding the multiple cellular processes mediated by RPGs. In this study, we investigated the functional architecture of the yeast RPS3 promoter and identified many putative cis-elements. Using beta-galactosidase reporter analysis and EMSA, the core promoter of RPS3 containing UASrpg and T-rich regions was corroborated. Moreover, the promoter occupancy of RPS3 by three transcription factors was confirmed. Taken together, our results further the current understanding of the promoter architecture and trans-elements of the Saccharomyces cerevisiae RPS3 gene.
Joo
Yoo Jin
YJ
Labotoray of Biochemistry, School of Life Sciences and Biotechnology, and BioInsititute, Korea University, Seoul 136-701, Korea.
Kim
Jin-Ha
JH
Baek
Joung Hee
JH
Seong
Ki Moon
KM
Lee
Jae Yung
JY
Kim
Joon
J
D024506:5' Flanking Region / Q000235:genetics; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000528:radiation effects; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
C495982:CRF1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C103154:HMO1 protein, S cerevisiae; D006609:High Mobility Group Proteins; D009841:Oligonucleotides; C440829:RAP1 protein, S cerevisiae; C501334:RPS3 protein, S cerevisiae; D012097:Repressor Proteins; D012269:Ribosomal Proteins; C117421:SFP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; D004317:Doxorubicin; D001616:beta-Galactosidase