[In vitro effect of secnidazole benzoate on Trichomonas vaginalis].
OBJECTIVE
To research the trichomonacidal effect of secnidazole benzoate in vitro.


METHODS
Trichomonas vaginalis was cultured in liver extract medium in 96-well microplate. The culture suspension of Trichomonas vaginalis was divided into four groups: secnidazole benzoate, secnidazole, metronidazole and control, with medium as blank control. MTT colorimetric assay was applied to determine the inhibitory effect of secnidazole benzoate on the proliferation of Trichomonas vaginalis. The culture suspension was transferred into test tubes and divided into same groups to observe inhibitory effect by the classical microscopic counting method.


RESULTS
After 24 h incubation, the proliferation of the parasites was concentration-dependent by secnidazole benzoate (t = 9.02, P < 0.01) at the concentration ranges from 0.15 microg/ml to 20.0 microg/ml with a relative inhibition rate (%) of 14.6, 28.7, 31.3, 60.4, 89.0, 89.2, 95.6, and 100.0 for MTT colorimetric assay, and 18.2, 31.1, 39.7, 68.8, 84.6, 90.1, 94.6, and 100.0 for counting method, respectively. In the period of 6-24 h incubation, the inhibition was in a time-dependent manner. The minimum sterilizing concentration and the minimum inhibitory concentration were 20 microg/ml and 0.15 microg/ml respectively.


CONCLUSIONS
Secnidazole benzoate shows a similar trichomonacidal effect to metronidazole and secnidazole.
Department of Pharmaceutical Engineering, Hefei University of Technology, Hefei 230009, China. zxxwyfzsy@sina.com
Xiao-Xiang
XX
Zhang
Shan-Shan
SS
Zheng
Mei-Mei
MM
Zhu
D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D066298:In Vitro Techniques; D008795:Metronidazole / Q000031:analogs & derivatives* / Q000494:pharmacology; D021261:Parasitic Sensitivity Tests; D014246:Trichomonas vaginalis / Q000187:drug effects*
D000994:Antitrichomonal Agents; D001565:Benzoates; D008795:Metronidazole; C016724:secnidazole