Phages specific for mycobacterial lipoarabinomannan help serodiagnosis of tuberculosis.
This study evaluated the possibility to use six phages specific to the Mycobacterium tuberculosis lipoarabinomannan (LAM) as tools for tubercular serodiagnosis. We analysed sera samples from 30 subjects with active tuberculosis (TB+), 30 with latent tubercular infection (LTBI) and 60 healthy subjects as controls (K). Our data indicated a good antibody response of the TB+ and LTBI patients against the phage Ri(7)17; the optical density (OD) values obtained from sera patients was statistically significant when compared to the control samples. Our results confirm that phage display technology might be useful to develop new tools for diagnosis of tuberculosis.
Bua
Alessandra
A
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy. ale.b76@email.it
Rosu
Valentina
V
Molicotti
Paola
P
Das Gupta
Sujoy K
SK
Ahmed
Niyaz
N
Zanetti
Stefania
S
Sechi
Leonardo A
LA
D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D019151:Peptide Library; D012698:Serologic Tests / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019151:Peptide Library; C050016:lipoarabinomannan