A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE.
A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated.
Laboratories of the International Health Division, The Rockefeller Foundation, New York.
T P
TP
Hughes
E G
EG
Pickels
F L
FL
Horsfall