Effect of an antioxidant functional food beverage on exercise-induced oxidative stress: a long-term and large-scale clinical intervention study.
The efficacy of long-term intake of a novel functional food supplement Funciona™ containing vitamins and juiced fruits was evaluated in order to assess the net effect of physical activity and antioxidant potentials in healthy older adult population. The long-term (2 years) and large-scale (400 older adult subjects) interventional study was based on both moderate-intensity exercise practice and concurrent supplementation. Sustained exercise-induced oxidative stress as reflected in significantly increased blood thiobarbituric acid-reactive substances (TBARS) (+15%), protein carbonyl groups (PC) (+18%) and oxidized glutathione (GSSG) (+112%) concentrations, and leukocyte 8-OHdG contents (23%). Exercise decreased the reduced/oxidized glutathione (GSH/GSSG) molar ratio (-43%) and plasma vitamin C levels (-22%). Supplementation with Funciona™ was significant in preventing oxidative damage to lipid, protein and DNA, and normalizing blood GSSG, GSH/GSSG and vitamin C levels. Thus daily intake of the antioxidant functional beverage counteracts the exercise-induced oxidative stress in free-living older subjects, and might be necessary to restore impaired antioxidant balance due long-term regular exercise.
Muñoz
Maria E
ME
Department of Physiology & Pharmacology, Campus M. Unamuno, University of Salamanca, E-37007 Salamanca, Spain.
Galan
Ana I
AI
Palacios
Encarna
E
Diez
Maria A
MA
Muguerza
Begoña
B
Cobaleda
Cesar
C
Calvo
Jose I
JI
Aruoma
Okezie I
OI
Sanchez-Garcia
Isidro
I
Jimenez
Rafael
R
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood; D015331:Cohort Studies; D019587:Dietary Supplements; D004435:Eating / Q000502:physiology; D005260:Female; D005978:Glutathione / Q000097:blood; D006147:Guanine / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D050050:Protein Carbonylation / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005978:Glutathione; D001205:Ascorbic Acid