Protective action of D-ribose against renal injury caused by ischemia and reperfusion in rats with transient hyperglycemia.
Hyperglycemia amplifies the inflammatory state after ischemia/reperfusion (I/R), and activated neutrophils have been implicated in the development of I/R-induced renal injuries. D-ribose is a naturally occurring monosaccharide found in all living cells. In this study, we examined whether D-ribose attenuates I/R-induced renal injury by reducing neutrophil activation in rats with transient hyperglycemia. Male Wistar rats were divided into sham (n = 24), control (n = 64), and D-ribose (n = 32) groups. Rats received intraperitoneal injection of glucose (3 g/kg) 30 min before induction of ischemia to induce transient hyperglycemia. Anesthetized rats underwent right nephrectomy and subsequent occlusion of the left renal artery and vein for 45 min. D-ribose (400 mg/kg) was intravenously administered 30 min before induction of ischemia. D-ribose significantly reduced the degree of the I/R-induced increases in renal concentrations of cytokine-induced neutrophil chemoattractant-1 (a chemotactic factor for the activation of neutrophils and chemotaxis to the site of injury) and myeloperoxidase (an indicator of neutrophils infiltration). D-ribose also reduced the I/R-induced increases in serum levels of blood urea nitrogen and creatinine, and improved histological changes, including acute tubular necrosis in the corticomedullary junction fields. These results indicate that D-ribose reduces the I/R-induced acute renal injury in rats with transient hyperglycemia, probably by reducing neutrophil activation. D-ribose might thus be useful for surgical procedures, such as renal transplant surgery, under hyperglycemia.
Department of Anesthesia and Perioperative Medicine, Kobe University Hospital Anesthesiology, Kobe, Japan.
Junji
J
Nishiyama
Masaaki
M
Ueki
Takehiko
T
Asaga
Kousuke
K
Chujo
Nobuhiro
N
Maekawa
D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism; D006943:Hyperglycemia / Q000150:complications* / Q000503:physiopathology; D007674:Kidney Diseases / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology* / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000503:physiopathology; D012266:Ribose / Q000627:therapeutic use*
D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D020011:Protective Agents; D012266:Ribose; D009195:Peroxidase