Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs.
Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA(Gln) is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro.
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom .
Christian
C
Cole
Andrew
A
Sobala
Cheng
C
Lu
Shawn R
SR
Thatcher
Andrew
A
Bowman
John W S
JW
Brown
Pamela J
PJ
Green
Geoffrey J
GJ
Barton
Gyorgy
G
Hutvagner
D001483:Base Sequence; D019295:Computational Biology; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000378:metabolism*
D035683:MicroRNAs; D034741:RNA, Small Interfering; D012343:RNA, Transfer; D043244:Ribonuclease III