[Interaction between mixed-lineage leukemia and Menin proteins].
The study was aimed to investigate the subcellular localization of mixed-lineage leukemia (MLL) and Menin proteins, and to explore the interaction between these two proteins. The recombinant eukaryotic cell expression vectors of pcDNA3.1-myc-MLL and pCMV-flag-Menin were constructed respectively, and transfected into the HEK293T cells. Immunofluorescence technique was used to observe the subcellular localization of the two proteins. Co-immunoprecipitation and Western blot methods were applied to evaluate the expression and interaction of the two proteins. The results showed that MLL and Menin proteins could be co-localized in cell nuclei, and the study of binding in vivo revealed that MLL protein could be detected in the immunoprecipitation complex of anti-FLAG, while Menin proteins could also be found in the immunoprecipitation complex of anti-MYC. It is concluded that MLL and Menin proteins co-localized in cell nuclei have same location and the interaction exists between MLL and Menin proteins.
Department of Hematology and Oncology, Shanghai Children Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Lin
L
Liu
Ben-Shang
BS
Li
Qi-Dong
QD
Ye
Ying-Yi
YY
He
Jing-Yan
JY
Tang
D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005822:Genetic Vectors; D057809:HEK293 Cells; D018398:Homeodomain Proteins; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D025941:Protein Interaction Mapping; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014162:Transfection
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C105373:MEN1 protein, human; D011518:Proto-Oncogene Proteins; D051788:Myeloid-Lymphoid Leukemia Protein