The priming effects of some human interleukins on the production of leukotrienes by calcium ionophore stimulated leukocytes.
This study demonstrates the changing levels of leukotrieneB4 (LTB4) and leukotrieneC4 (LTC4) generated by human white blood cells primed with different human interleukins IL-3, IL-5, IL-6, IL-8 and with human hematopoietic growth factor granulocyte-macrophage colony stimulating factor (GM-CSF). Preincubation of white blood cells at 37 degrees C for 30 min with different human interleukins significantly increased leukotriene production in white blood cells preincubated with diluent control while IL-3 was without priming effects. Addition of exogenous arachidonic acid to non primed white blood cells increased the production of leukotrienes in response to the calcium ionophore (A23187). The enhancement of the leukotrienes production by white blood cells stimulated by A23187 was only observed when the cells stimulated for periods of 2 min or less. The results reveal that GM-CSF, IL-4, IL-6 and IL-8 belong to particular set of cytokines with potent modulating effect on inducing inflammatory mediators released by white blood cells, while IL-3 was ineffctive.
Tumor Marker Oncology Research Center, Al-Azhar University, Cairo, Egypt.
M
M
Mansour
O
O
El-Ahmady
T
T
Motawi