Ectopic expression of hC/EBPs in breast tumor cells induces apoptosis.
CCAAT/enhancer binding proteins (C/EBPs) are a group of transcription factors which have been implicated in cellular proliferation, terminal differentiation, and apoptosis in a variety of tissues including mammary gland. Owing to its role in various cellular functions, inactivation of C/EBP proteins is central to the pathogenesis of many disorders. Recent reports suggest that expression as well as function of C/EBP proteins is deregulated in breast tumors. Although, role of C/EBPs in growth arrest in mammary tissues has been studied in much detail; their role in apoptosis is relatively less explored. In the present study, we have assessed if breast tumors evade apoptosis and grow faster by down regulating and inhibiting the C/EBP proteins, C/EBPalpha in particular. Our data shows that ectopic expression of human C/EBPs in breast tumor cells inhibits proliferation and induces apoptosis which is likely to be associated with caspase dependent pathway.
Pal
Pooja
P
Drug Target Discovery and Development Division (DTDD), Central Drug Research Institute, CDRI, Chhattar Manzil, Lucknow, UP, India.
Lochab
Savita
S
Kanaujiya
Jitendra
J
Sanyal
Sabyasachi
S
Trivedi
Arun Kumar
AK
D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000502:physiology; D049109:Cell Proliferation; D053938:DNA Fragmentation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology
D051017:Apoptosis Regulatory Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D011065:Poly(ADP-ribose) Polymerases