Lymph node stromal cells support dendritic cell-induced gut-homing of T cells.
T cells are imprinted to express tissue-specific homing receptors upon activation in tissue-draining lymph nodes, resulting in their migration to the site of Ag entry. Expression of gut-homing molecules alpha(4)beta(7) and CCR9 is induced by retinoic acid, a vitamin A metabolite produced by retinal dehydrogenases, which are specifically expressed in dendritic cells as well as stromal cells in mucosa-draining lymph nodes. In this study, we demonstrate that mesenteric lymph node stromal cell-derived retinoic acid can directly induce the expression of gut-homing molecules on proliferating T cells, a process strongly enhanced by bone marrow-derived dendritic cells in vitro. Therefore, cooperation of sessile lymph node stromal cells with mobile dendritic cells warrants the imprinting of tissue specific homing receptors on activated T cells.
Department of Molecular Cell Biology and Immunology, VU (Vrije Universiteit) University Medical Center, Amsterdam, The Netherlands.
Rosalie
R
Molenaar
Mascha
M
Greuter
Arnold P J
AP
van der Marel
Ramon
R
Roozendaal
Stefan F
SF
Martin
Fanny
F
Edele
Jochen
J
Huehn
Reinhold
R
Förster
Tom
T
O'Toole
Wendy
W
Jansen
Inge L
IL
Eestermans
Georg
G
Kraal
Reina E
RE
Mebius
D000444:Aldehyde Dehydrogenase / Q000276:immunology / Q000378:metabolism; D000818:Animals; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D016023:Integrins / Q000276:immunology* / Q000378:metabolism; D007422:Intestines / Q000276:immunology*; D008198:Lymph Nodes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D054388:Receptors, CCR / Q000276:immunology / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
C119309:CC chemokine receptor 9; D016023:Integrins; D054388:Receptors, CCR; C071777:integrin alpha4beta7; D000444:Aldehyde Dehydrogenase