Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells.
The aim of the current study was to visualize new bone formed in vivo on a small intestine submucosa (SIS) sponge used as a tissue-engineered scaffold for the repair of damaged bone. The SIS sponge provided a three-dimensional pore structure, and supported good attachment and viability of rat bone marrow stem cells (rBMSCs). To examine bone regeneration, we prepared full-thickness bilateral bone defects in the rat crania, and then treated the defects with an implanted SIS sponge or PGA mesh without or with rBMSCs, or left the defects untreated. Bone defects were evaluated by micro-CT and histologically after 2 and 4 weeks. Micro-CT demonstrated a trend toward a decrease in bone void in both the SIS sponge and SIS sponge/rBMSCs groups compared to the control and PGA mesh groups. At 4 weeks, bone formation in defects containing SIS sponge/rBMSCs was significantly greater than in all other groups. A histological analysis after 2 and 4 weeks of implantation showed localized collagen and osteocalcin deposition on SIS sponges and SIS sponges with rBMSCs. These in vivo results indicate that the SIS sponge, implanted at bone-removal defects, facilitated bone regeneration.
Kim
Kyung Sook
KS
Nano Bio Fusion Research Center, Korea Research Institute of Chemical Technology, P. O. Box 107, Yuseung, Daejeon 305-600, Republic of Korea.
Lee
Ju Young
JY
Kang
Yun Mi
YM
Kim
E Sle
ES
Kim
Gyeong Hae
GH
Rhee
Sang Dal
SD
Cheon
Hyae Gyeong
HG
Kim
Jae Ho
JH
Min
Byoung-Hyun
BH
Lee
Hai Bang
HB
Kim
Moon Suk
MS
D000818:Animals; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D007413:Intestinal Mucosa / Q000637:transplantation*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010012:Osteogenesis; D011859:Radiography; D051381:Rats; D011916:Rats, Inbred F344; D012887:Skull Fractures / Q000000981:diagnostic imaging / Q000601:surgery*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome