Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern.
Two extremely halophilic archaea, strains RO5-2(T) and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2(T) and RO5-14 were able to grow at 20-50 degrees C (optimum 37 degrees C), at 2.6-4.8 M NaCl (optimum 3.4-3.9 M NaCl), at 0.03-0.7 M MgCl(2) (optimum 0.5 M MgCl(2)) and at pH 5.5-8.0 (optimum pH 6.5-7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2(T) and RO5-14 showed 93.4-93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2(T) and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA-DNA relatedness between strains RO5-2(T) and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2(T) and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2(T) (=CGMCC 1.7739(T) =JCM 15773(T)).
Cui
Heng-Lin
HL
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Li
Xin-Yi
XY
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Gao
Xia
X
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Xu
Xue-Wei
XW
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Zhou
Yu-Guang
YG
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Liu
Hong-Can
HC
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Oren
Aharon
A
Institute of Life Sciences, and the Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Zhou
Pei-Jin
PJ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride