Penetration of cefuroxime in subcutaneous tissue during coronary artery bypass grafting surgery.
A sensitive and rapid HPLC assay for determining cefuroxime penetration in the subcutaneous tissue near to surgical incision of patients submitted to coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass (CPB) was performed. Blood and subcutaneous tissue samples were collected from 14 patients, in four periods during surgery. The analytical method presented linearity from 0.5 to 100 microg/g, LOQ=0.50 microg/g, LOD=0.25 microg/g, intra- and interday precision (%CV) ranged from 4.9 to 8.9% and 6.4 to 9.9%, respectively, and intra- and interday accuracy expressed as % of the nominal concentration ranged from 87.1 to 104.6% and 94.8 to 103.8%, respectively (mean of three concentrations). Relative recovery was 98.4%. Tissue/plasma ratios obtained for CPB and non-CPB were, respectively: 14.6% vs 19.0% (0.6 h); 15.7% vs 15.7% (2.1 h); 22.5% vs 19.9% (3.6 h); 15.7% vs 18.8% (4.5 h). Data obtained indicate that tissue/plasma ratio remains unchanged in CPB and non-CPB patients during all period of surgery and the CPB does not affect the penetration of cefuroxime in tissues close to the surgical wound.
Pharmacy Department, School of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 580, 05508-000 Sao Paulo, SP, Brazil. jorgewillian@uninove.br
Jorge Willian Leandro
JW
Nascimento
Maria José Carvalho
MJ
Carmona
Tânia Mara Varejão
TM
Strabelli
José Otávio Costa
JO
Auler
Sílvia Regina Cavani Jorge
SR
Santos
D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000097:blood*; D002444:Cefuroxime / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D001026:Coronary Artery Bypass / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged
D000900:Anti-Bacterial Agents; D002444:Cefuroxime