Pulsed electromagnetic fields accelerate proliferation and osteogenic gene expression in human bone marrow mesenchymal stem cells during osteogenic differentiation.
Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined the effect of pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and gene expression in bone marrow mesenchymal stem cells (BMMSCs) during osteogenic differentiation. Exposure of BMMSCs to PEMFs increased cell proliferation by 29.6% compared to untreated cells at day 1 of differentiation. Semi-quantitative RT-PCR indicated that PEMFs significantly altered temporal expression of osteogenesis-related genes, including a 2.7-fold increase in expression of the key osteogenesis regulatory gene cbfa1, compared to untreated controls. In addition, exposure to PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis. These results suggest that PEMFs enhance early cell proliferation in BMMSC-mediated osteogenesis, and accelerate the osteogenesis.
Sun
Li-Yi
LY
Department of Biological Science and Technology, National Chiao Tung University, No. 75 Po-Ai Street, Hsinchu, Taiwan, ROC.
Hsieh
Dean-Kuo
DK
Lin
Po-Cheng
PC
Chiu
Hsien-Tai
HT
Chiou
Tzyy-Wen
TW
D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology / Q000528:radiation effects*; D002113:Calcification, Physiologic / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004574:Electromagnetic Fields; D005109:Extracellular Matrix / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology / Q000528:radiation effects*; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*; D010507:Periodicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D050659:Core Binding Factor Alpha 1 Subunit; D000469:Alkaline Phosphatase