Trimethylated chitosan-conjugated PLGA nanoparticles for the delivery of drugs to the brain.
Trimethylated chitosan (TMC) surface-modified poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (TMC/PLGA-NP) were synthesized as a drug carrier for brain delivery. TMC was covalently coupled to the surface of PLGA nanoparticles (PLGA-NP) via a carbodiimide-mediated link. The zeta potential of TMC/PLGA-NP was about 20mV with a mean diameter around 150nm. 6-coumarin loaded PLGA-NP and TMC/PLGA-NP were injected into the caudal vein of mice, and fluorescent microscopy of brain sections showed a higher accumulation of TMC/PLGA-NP in the cortex, paracoele, the third ventricle and choroid plexus epithelium, while no brain uptake of PLGA-NP was observed. There was no pronounced difference in cell viability between TMC/PLGA-NP and PLGA-NP as shown by MTT assay. Behavioral testing showed that the injection of coenzyme Q(10) loaded TMC/PLGA-NP greatly improved memory impairment, restoring it to a normal level, but the efficacy was slight for loaded PLGA-NP, without TMC conjugation. The senile plaque and biochemical parameter tests confirmed the brain-targeted effects of TMC/PLGA-NP. These experiments show that TMC surface-modified nanoparticles are able to cross the blood-brain barrier and appear to be a promising brain drug delivery carrier with low toxicity.
Wang
Zhao H
ZH
Department of Pharmaceutics, Shenyang Pharmaceutical University, Liaoning, China.
Wang
Zhan Y
ZY
Sun
Chang S
CS
Wang
Chun Y
CY
Jiang
Tong Y
TY
Wang
Si L
SL
D000818:Animals; D001921:Brain / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics*; D019344:Lactic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D008657:Metabolic Clearance Rate; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014018:Tissue Distribution
D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan