Vitamin A supplementation induces oxidative stress and decreases the immunocontent of catalase and superoxide dismutase in rat lungs.
Lungs require an adequate supply of vitamin A for normal embryonic development, postnatal maturation, and maintenance and repair during adult life. However, recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer, although the mechanisms underlying this effect are still unknown. Here, the authors studied the effect of vitamin A supplementation on oxidative stress parameters in lungs of Wistar rats. Vitamin A supplementation either at therapeutic (1000 and 2500 IU/kg) or excessive (4500 and 9000 IU/kg) doses for 28 days induced lipid peroxidation, protein carbonylation, and oxidation of protein thiol groups, as well as change in catalase (EC 1.11.1.6; CAT) and superoxide dismutase (EC 1.15.1.1, SOD) activities and immunocontents. These results altogether suggest that vitamin A supplementation causes significant changes in redox balance the free radical status in lungs, which are frequently associated to severe lung dysfunction.
Pasquali
Matheus A B
MA
Centro de Estudos em Estresse Oxidativo, Universidade Federal do Rio Grande do Sul, Departamento de Bioquímica, Porto Alegre, Brazil.
Gelain
Daniel P
DP
Oliveira
Marcos R
MR
Behr
Guilherme A
GA
Motta
Leonardo L
LL
Rocha
Ricardo F
RF
Klamt
Fábio
F
Moreira
José C F
JC
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D019587:Dietary Supplements / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D015151:Immunoblotting; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology* / Q000633:toxicity
D013438:Sulfhydryl Compounds; D014801:Vitamin A; D002374:Catalase; D013482:Superoxide Dismutase