RNA interference targeting nucleocapsid protein (C) inhibits classical swine fever virus replication in SK-6 cells.
The application of RNA interference (RNAi) strategy for controlling classical swine fever could become a promising alternative to the conventional eradication measures, as it was recently shown for foot-and-mouth disease (Chen et al., 2004), influenza (Ge et al., 2003), porcine reproductive and respiratory syndrome (He et al., 2007) and porcine transmissible gastroenteritis (Zhou et al., 2007). The use of synthetic siRNA which is corresponding to nucleotides 1130-1148 of the CSF virus strain Alfort, targeting the nucleocapsid protein (C) was investigated to show the inhibition of CSF virus replication. It could be shown that the virus titer of infected cells, which had been mock-transfected or transfected with control (non-silence) RNA were not affected. These data indicate that siRNA_253 is able to inhibit viral replication.
Department of Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. Sarthorn@kku.ac.th
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Porntrakulpipat
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Supankong
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Chatchawanchonteera
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Pakdee
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D006691:Classical Swine Fever / Q000378:metabolism*; D006692:Classical Swine Fever Virus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D003601:Cytotoxicity Tests, Immunologic; D050296:Microbial Viability / Q000187:drug effects; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D013552:Swine; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering