Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata.
Telomerase, the key enzyme essential for the maintenance of eukaryotic chromosome ends, contains a reverse transcriptase and an RNA that provides the template for the synthesis of telomeric repeats. Here, we characterize the telomerase subunits in the hemiascomycete yeast Candida glabrata. We propose a secondary structure model for the telomerase RNA that is the largest described to date. Telomerase deletion mutants show a progressive shortening of telomeres and a modest loss of viability. Frequent post-senescence survivors emerge that possess long telomeric repeat tracts. We suggest that the high telomere length heterogeneity accounts for this distinct senescence phenotype.
Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 67084 Strasbourg, France.
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Kachouri-Lafond
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Dujon
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Gilson
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Westhof
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Fairhead
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Teixeira
D001483:Base Sequence; D015139:Blotting, Southern; D041221:Candida glabrata / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division; D004271:DNA, Fungal / Q000235:genetics; D005434:Flow Cytometry; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D019098:Telomerase / Q000737:chemistry / Q000235:genetics*; D016615:Telomere / Q000235:genetics*
D004271:DNA, Fungal; D012331:RNA, Fungal; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase