Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier.
A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM NDA, 20 mM borate buffer (pH 9.2) with the reaction time of 4 min at room temperature. The capillary electrophoresis analysis of GSH could be achieved in less than 120 s using 10 mM sodium tetraborate (pH 9.2) containing 2.5 mM beta-cyclodextrin (beta-CD) as the running buffer, and the detection limit of 2.5 x 10(-9) M (S/N=3) was obtained. This method was successfully applied to analyze the content of GSH in tobacco BY-2 cells.
Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China. lyzhang26@yahoo.com.cn
Li-Yao
LY
Zhang
Meng-Xiang
MX
Sun
D002021:Buffers; D002460:Cell Line; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D009281:Naphthalenes / Q000737:chemistry; D014026:Nicotiana; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002021:Buffers; D009281:Naphthalenes; D047392:beta-Cyclodextrins; C027717:2,3-naphthalenedicarboxaldehyde; D005978:Glutathione; C031215:betadex