QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : EFFECT OF SALTS ON THE REACTION.
1. A quantitative study has been made of the effect on the precipitin reaction between the specific polysaccharide of Type III pneumococcus and the homologous antibody of salt concentrations ranging from O.1 M to 1.79 M, including the effect of ions of higher valence. 2. Within these limits, observed decreases in precipitated antibody with increasing salt concentration appear to be due to a decrease in the amount of antibody combined with the S III, rather than to an increase in solubility of the S III-antibody compounds. 3. The egg albumin-antibody reaction is far less sensitive to changes in salt concentration than is the S III-antibody reaction.
Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York.
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M
Heidelberger
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FE
Kendall
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T
Teorell