Monoclonal antibodies against the connexin43-interacting protein CIP85.
The connexin43 (Cx43)-interacting protein of 85 kDa CIP85 has been identified as an interacting partner for the cytoplasmically located, carboxyl-terminal tail of Cx43. Further characterization has shown that the interaction between Cx43 and CIP85 is associated with increased turnover of Cx43 that may be lysosome-mediated. This suggests that CIP85 may regulate the endocytic trafficking of Cx43 from the plasma membrane and its degradation, and thus, indirectly influence gap junction function. This study reports the first successful production of monoclonal antibodies (MAbs) against CIP85. These antibodies are useful in detecting CIP85 expressed in several species in immunoblotting, immunoprecipitation, and immunofluorescence microscopy experiments. These MAbs will assist in defining the functional roles of CIP85, including its influence on Cx43 trafficking and intercellular communication through Cx43-containing gap junctions.
Natural Products and Cancer Biology Program, Cancer Research Center of Hawaii, Honolulu, Hawaii, USA.
Kimberly
K
Cochrane
John M
JM
Berestecky
Carolynn
C
Kitamura
Alan F
AF
Lau
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D020691:rab GTP-Binding Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; C499824:CIP85 protein, mouse; D020691:rab GTP-Binding Proteins