Placental features of chorioamnionitis colonized with Ureaplasma species in preterm delivery.
Ureaplasma spp. is detected in the urogenital tract, including the vagina, cervix, chorioamnion, and placenta. Their colonization is associated with histologic chorioamnionitis (CAM), often observed in placentas from preterm delivery. We isolated Ureaplasma spp. from 63 preterm placentas among 151 specimens, which were delivered at <32 wk of gestation. Of the 63 placentas, 52 (83%) revealed CAM in cultures positive for Ureaplasma spp., however, CAM was observed only in 30% (26/88) of cultures negative for Ureaplasma spp. (p < 0.01). Colonization by Ureaplasma spp. was an independent risk factor for CAM (OR, 11.27; 95% CI, 5.09-24.98). Characteristic neutrophil infiltration was observed in the amnion and subchorion (bistratified pattern) in cultures positive for Ureaplasma spp. FISH analysis of CAM placenta with male infant pregnancy indicated that bistratified infiltrated neutrophils showed the XX karyotype and umbilical vein infiltrated neutrophils showed XY karyotype. The distribution of sulfoglycolipid, the receptor of Ureaplasma spp., was mainly detected in the amnion. Ureaplasmal urease D protein and ureB gene were both detected in the amnion, indicating direct colonization by Ureaplasma spp.
Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka 594-1101, Japan.
Fumihiko
F
Namba
Taeko
T
Hasegawa
Masahiro
M
Nakayama
Takuro
T
Hamanaka
Tatsuyuki
T
Yamashita
Kumiko
K
Nakahira
Akihito
A
Kimoto
Masatoshi
M
Nozaki
Masahiro
M
Nishihara
Kazuya
K
Mimura
Minoru
M
Yamada
Hiroyuki
H
Kitajima
Noriyuki
N
Suehara
Itaru
I
Yanagihara
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002821:Chorioamnionitis / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D005260:Female; D005865:Gestational Age; D006017:Glycolipids / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D016015:Logistic Models; D008297:Male; D020556:Neutrophil Infiltration; D016017:Odds Ratio; D010920:Placenta / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D047928:Premature Birth / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D018570:Risk Assessment; D012307:Risk Factors; D014509:Ureaplasma / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016869:Ureaplasma Infections / Q000150:complications / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D014510:Urease / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D006017:Glycolipids; C011117:sulfoglycolipids; D014510:Urease