Adhesion, migration and mechanics of human adipose-tissue-derived stem cells on silk fibroin-chitosan matrix.
Silk fibroin-chitosan (SFCS) scaffold is a naturally derived biocompatible matrix with potential reconstructive surgical applications. In this study, human adipose-derived mesenchymal stem cells (ASCs) were seeded on SFCS scaffolds and cell attachment was characterized by fluorescence, confocal, time-lapse, atomic force, and scanning electron microscopy (SEM) studies. Adhesion of ASCs on SFCS was 39.4 + or - 4.8% at 15 min, increasing to 92.8+/-1.5% at 120 min. ASC adhered at regions of architectural complexity and infiltrate into three-dimensional scaffold. Time-lapse confocal studies indicated a mean ASC speed on SFCS of 18.47+/-2.7 microm h(-1) and a mean persistence time of 41.4 + or - 9.3 min over a 2.75 h study period. Cytokinetic and SEM studies demonstrated ASC-ASC interaction via microvillus extensions. The apparent elastic modulus was significantly higher (p<0.0001) for ASCs seeded on SFCS (69.0 + or - 9.0 kPa) than on glass (6.1 + or - 0.4 kPa). Also, cytoskeleton F-actin fiber density was higher (p<0.05) for ASC seeded on SFCS (0.42 + or - 0.02 fibers microm(-1)) than on glass-seeded controls (0.24 + or - 0.03 fibers microm(-1)). Hence, SFCS scaffold facilitates mesenchymal stem cell attachment, migration, three-dimensional infiltration, and cell-cell interaction. This study showed the potential use of SFCS as a local carrier for autologous stem cells for reconstructive surgery application.
Department of Plastic Surgery, The University of Texas M. D. Anderson Cancer Center, P.O. Box 301402, Unit 602, Houston, TX 77230-1402, USA.
Andrew M
AM
Altman
Vishal
V
Gupta
Carmen N
CN
Ríos
Eckhard U
EU
Alt
Anshu B
AB
Mathur
D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D001696:Biomechanical Phenomena / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D047011:Silk / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors; D054457:Tissue Scaffolds
D000199:Actins; D047011:Silk; D049452:Green Fluorescent Proteins; D005349:Fibroins; D048271:Chitosan