Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry.
Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification.
Peterson
Robyn
R
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. rpeterson@chem.mq.edu.au
Grinyer
Jasmine
J
Joss
Janice
J
Khan
Alamgir
A
Nevalainen
Helena
H
D003224:Congo Red / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology; D008361:Mannosidases / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*
D005656:Fungal Proteins; D003224:Congo Red; D008361:Mannosidases