[Clinical evaluation of the application of gene chip for identifying pathogens in blood cultures].
OBJECTIVE
To explore the feasibility of using gene chip method to identify pathogens in blood cultures.


METHODS
Clinical blood samples were obtained and cultured using an automated blood culture system. A gene chip diagnostic kit was used to detect the pathogenic bacteria in these blood cultures following the procedures of target gene extraction and amplification, hybridization and result analysis. The conventional method was also used to isolate and identify the bacteria from the clinical blood cultures, and the results of the two methods were compared.


RESULTS
In the 86 clinical blood samples, 74 were positive and 12 negative according to the conventional method, while 48 were positive and 38 negative as found by the gene chip method, showing significant differences in the results (P<0.05). The two methods only had a concordance rate of 69.77%.


CONCLUSIONS
The gene chip diagnostic kit has low concordance rate with the conventional method for detecting pathogens in clinical blood cultures and awaits further improvement.
Department of Clinical Laboratory, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. mature303@126.com
Lin-hai
LH
Li
Ying
Y
Cheng
Li-dan
LD
Chen
Xiao-yan
XY
Huang
Yu-ling
YL
Shi
Jie-jing
JJ
He
Lu-xia
LX
Wang
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S