[Metabolism characteristics of Chelativorans oligotrophicus by two-phase growth on the mixture of EDTA and glucose].
The obligate destructor of ethylene diamine tetraacetate--a culture of Chelativorans oligotrophicus LPM-4--did not grow on a medium with glucose, but it was good to use it under cultivation on a mixture with EDTA after considerable decrease of the EDTA concentration in the medium (two-phase growth). Strong inhibition of hexokinase and glucose 6-phosphate dehydrogenase in cell exracts 4 mM EDTA was revealed. Using EDTA, cells accumulated polyphosphates whose rate decreased during glucose utilization phase. High activities of polyphosphate biosynthesis ferments (adenylat kinase and polyphosphate kinase) were distinguished during the first phase of the cultivation; considerable decrease of them and increase of polyphosphate glucokinase were found during the second phase of the cultivation. This points to the possible participating of polyphosphates in glucose metabolism as a supplementary energy source.
E N
EN
Kaparullina
N V
NV
Doronina
L V
LV
Trilisenko
V M
VM
Vagabov
Iu A
IuA
Trotsenko
D000263:Adenylate Kinase / Q000378:metabolism; D020561:Alphaproteobacteria / Q000201:enzymology / Q000254:growth & development*; D001426:Bacterial Proteins / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism; D013549:Sweetening Agents / Q000494:pharmacology*
D001426:Bacterial Proteins; D002614:Chelating Agents; D013549:Sweetening Agents; D004492:Edetic Acid; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D000263:Adenylate Kinase; D005947:Glucose