Reelin-disabled-1 signaling in the mature rat cochlear nucleus.
CONCLUSIONS
Immunohistochemical detection of Reelin in granular cells and disabled-1 in cochlear nucleus suggests a possible Reelin signaling pathway in mature rat cochlear nucleus.


METHODS
Six-week-old Wister rats were used throughout this study. The expression of reelin and disabled-1 were studied by using in situ hybridization technique and immunohistochemistry.


RESULTS
Reelin mRNA expression was observed in granular cell layer of dorsal cochlear nucleus. Immunohistochemistry using anti-reelin monoclonal antibodies confirmed reelin expression in granule cells at protein level. We also examined disabled-1 expression in cochlear nucleus and observed positive immunoreactivity in both ventricular and dorsal cochlear nucleus. In the dorsal cochlear nucleus, fusiform and cartwheel cells were labeled. In the ventricular cochlear nucleus, relatively large cells were labeled with anti-disabled-1 polyclonal antibody but the subtypes of disabled-1 positive cells could not be identified.
Inagaki
Akira
A
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Nagoya City University, Nagoya-City, Japan. ainagaki@med.nagoya-cu.ac.jp
Ugawa
Shinya
S
Safwat
Majid M
MM
Keceli
Sumru
S
Shimada
Shoichi
S
Motohiko
Suzuki
S
Murakami
Shingo
S
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D017626:Cochlear Nucleus / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism*; D015398:Signal Transduction
D048868:Adaptor Proteins, Signal Transducing; D015816:Cell Adhesion Molecules, Neuronal; C499786:Dab1 protein, rat; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000091042:Reelin Protein; C000717129:Reln protein, rat; D012697:Serine Endopeptidases