Controlled gating of lysenin pores.
Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
Fologea
Daniel
D
Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, USA. dfologea@uark.edu
Krueger
Eric
E
Lee
Rachel
R
Naglak
Matt
M
Mazur
Yuriy
Y
Henry
Ralph
R
Salamo
Greg
G
D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000737:chemistry*; D013109:Sphingomyelins; D014118:Toxins, Biological / Q000737:chemistry*
D008051:Lipid Bilayers; D013109:Sphingomyelins; D014118:Toxins, Biological; C107040:lysenin