A developmental study of enteric neuron migration in the grasshopper using immunological probes.
Motility of enteric plexus neurons in the grasshopper Locusta migratoria depends critically on the NO/cGMP signaling cascade. This is reflected in a strong NO-dependent cGMP staining in migrating enteric midgut neurons. In contrast, first cGMP immunoreactivity (cGMP-IR) in the foregut enteric ganglia was detected clearly after the main migratory processes have taken place. Thus, expression of cGMP-IR in migrating neurons is a distinct phenomenon restricted to neurons forming midgut and foregut plexus, that does not occur during convergence of neurons forming the enteric ganglia. Analysis of time lapse video microscopy of migrating midgut neurons together with an immunofluorescence study of midgut cellular structures suggests a contribution of the midgut musculature to enteric neuron guidance. Additionally, during midgut plexus formation a fasciculating signal for enteric neuron neurites appears to be provided by the cell adhesion molecule Fasciclin I.
Knipp
Sabine
S
University of Veterinary Medicine Hannover, Division of Cell Biology, Institute of Physiology, Hannover, Germany.
Bicker
Gerd
G
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002465:Cell Movement; D006152:Cyclic GMP / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D017615:Enteric Nervous System / Q000196:embryology* / Q000378:metabolism; D006110:Grasshoppers / Q000196:embryology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000196:embryology* / Q000294:innervation; D009132:Muscles / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism
D015816:Cell Adhesion Molecules, Neuronal; C051641:fasciclin I; D009569:Nitric Oxide; D006152:Cyclic GMP