Possible effects of interferon-alpha on Fas-induced renal apoptosis in mouse.
Fas (APO-1/CD95) is a cell surface receptor that initiates apoptotic pathway. Fas-stimulated ROS generation may play important role in Fas-mediated apoptosis. The aim of this study was to evaluate the influence of interferon-alpha on oxidative stress parameters in Fas-induced renal apoptosis in mice kidney.


METHODS
One-month-old Balb C male mice were used for the study. The animals were divided in four groups: group 1 were the controls, group 2 mice were treated with anti-Fas antibody i.p., group 3 mice were treated with IFN-alpha, and group 4 mice were treated with both agents simultaneously. The mice were killed 48 h afterwards, and kidneys were homogenized. TBA reactive substances (TBARS), glutathione content, and reactive carbonyl group (RCG) were measured.


RESULTS
The results showed a statistically significant increase of TBARS (p < 0.05) and RCG (p < 0.05) concentration in the group treated with anti-Fas antibody versus control. IFN-alpha decreased the concentration of TBARS and RCG after anti-Fas antibody administration (p < 0.05). There is no significant difference in glutathione content between investigated groups.


CONCLUSIONS
IFN-alpha might be considered as a new target for therapeutic intervention in FasL/Fas induced renal injury.
Institute of Biochemistry, Medical Faculty, University of Nis, Serbia. tanja_cvetkovic@yahoo.co.uk
T
T
Cvetkovic
G
G
Kocic
T
T
Jevtovic-Stoimenov
D
D
Pavlovic
J
J
Basic
A
A
Topalovic
A
A
Veljkovic
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D016898:Interferon-alpha / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D018709:Statistics, Nonparametric; D014508:Urea / Q000097:blood; D019014:fas Receptor / Q000494:pharmacology*
D016898:Interferon-alpha; D019014:fas Receptor; D014508:Urea; D003404:Creatinine