Anti-invasive activity of anthocyanins isolated from Vitis coignetiae in human hepatocarcinoma cells.
We investigated anti-invasive effects of the anthocyanins from fruits of Vitis coignetiae Pulliat (known as meoru in Korea) on human hepatoma Hep3B cells. The anthocyanins inhibited cell invasion in a dose-dependent manner as measured by Matrigel (BD Biosciences, San Jose, CA, USA) invasion assays. They also inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9 and activation of nuclear factor kappaB (NF-kappaB) stimulated by tumor necrosis factor alpha. Taken together, the results of this study indicate that the anthocyanins isolated from fruits of V. coignetiae Pulliat have anti-invasive effects on human hepatoma Hep3B cells and inhibit MMP-2 and MMP-9 gene expression at least in part through the inhibition of NF-kappaB activation.
Department of Biochemistry, Dongeui University College of Oriental Medicine, Jeju, Republic of Korea.
Dong Yeok
DY
Shin
Won Sup
WS
Lee
Seok Hyun
SH
Kim
Min Jeong
MJ
Kim
Jeong Won
JW
Yun
Jing Nan
JN
Lu
Sung Joong
SJ
Lee
Irina
I
Tsoy
Hye Jung
HJ
Kim
Chung Ho
CH
Ryu
Gi Young
GY
Kim
Ho Sung
HS
Kang
Sung Chul
SC
Shin
Yung Hyun
YH
Choi
D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009361:Neoplasm Invasiveness; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; D010936:Plant Extracts