Analytical approaches to uptake and release of hydrogel-associated FGF-2.
Strategies to control the delivery of growth factors are critically important in the design of advanced biomaterials. In this study we investigated the binding and release of fibroblast growth factor 2 (FGF-2) to/from a biohybrid hydrogel matrix by four independent analytical methods: radioisotope and fluorescence labeling, amino acid analysis and Enzyme-Linked Immunosorbent Assays (ELISA). The compared analyses provided qualitatively similar uptake characteristics while the results of the FGF-2 quantification strongly depended on the particular experimental conditions. The release kinetics of FGF-2 from the gels could be monitored sensitively by (125)I labeling and by ELISA-techniques. The latter method was concluded to be advantageous since it permits the application of unmodified ("native") growth factors.
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden & Technical University Dresden, Center for Regenerative Therapies Dresden, Dresden, Hohe Str. 6, 01069, Dresden, Germany. zieris@ipfdd.de
A
A
Zieris
S
S
Prokoph
P B
PB
Welzel
M
M
Grimmer
K R
KR
Levental
W
W
Panyanuwat
U
U
Freudenberg
C
C
Werner
D001672:Biocompatible Materials / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007457:Iodine Radioisotopes / Q000737:chemistry; D007700:Kinetics; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding; D013997:Time Factors; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D020100:Hydrogels; D007457:Iodine Radioisotopes; D014867:Water; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D006493:Heparin