Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebrafish (Danio rerio).
Genomic and cDNA sequences coding for a fatty acid-binding protein (FABP) in zebrafish were retrieved from DNA sequence databases. The cDNA codes for a protein of 14.7 kDa (pI = 5.94), and the gene consists of four exons, properties characteristic of most vertebrate FABP genes. Phylogenetic analyses using vertebrate FABPs indicated that this protein is most similar to zebrafish Fabp10. Currently, only one fabp10 gene is annotated in the zebrafish genome. In this article, the notations 'fabp10a' and 'fabp10b' are used to refer to the duplicate copies of fabp10. The zebrafish fabp10a and fabp10b genes were assigned by radiation hybrid mapping to chromosomes 16 and 19, respectively. On the basis of conserved gene synteny with chicken FABP10 on chromosome 23, zebrafish fabp10a and fabp10b are duplicates resulting from a whole-genome duplication event early in the ray-finned fish lineage some 230-400 million years ago. Whole-mount in situ hybridization detected fabp10b transcripts only in the olfactory vesicles of embryos and larvae, whereas fabp10a transcripts have been shown previously to be present only in the liver of embryos and larvae. In adults, RT-PCR detected fabp10b transcripts in all tissues assayed. By contrast, fabp10a transcripts were detected only in adult liver, intestine and testis. This differential tissue distribution of transcripts for the duplicated fabp10 genes suggests considerable divergence of their cis-acting regulatory elements since their duplication.
Venkatachalam
Ananda B
AB
Department of Biology, Dalhousie University, Halifax, Canada.
Thisse
Christine
C
Thisse
Bernard
B
Wright
Jonathan M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000235:genetics; D004625:Embryo, Nonmammalian; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication; D018507:Gene Expression Regulation, Developmental; D007413:Intestinal Mucosa / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D050556:Fatty Acid-Binding Proteins; D029961:Zebrafish Proteins; C513422:fatty acid-binding protein 10, zebrafish