Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone.
Different cell disintegration methods were used for the liberation of intracellular carbonyl reductase from Geotrichum candidum, in its active form. Solid shear (bead milling) was proved to be the best method for the extraction of the enzyme. Various solid supports were checked for the immobilization of the purified enzyme. Carbonyl reductase was immobilized on silica with an optimized protein loading of 4 mg/g support. Cross-linking with glutaraldehyde rendered the preparation more stable and suitable for use in consecutive batches. Carbonyl reductase of G. candidum immobilized on silica support and cross-linked by glutaraldehyde was found to be highly efficient biocatalyst formulation for the production of S(-)-1-(1'-naphthyl) ethanol.
Department of Pharmaceutical Technology Biotechnology, National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar-160062, Punjab, India.
Mani Shankar
MS
Bhattacharyya
Amit
A
Singh
U C
UC
Banerjee
D000327:Adsorption; D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005848:Geotrichum / Q000201:enzymology*; D005976:Glutaral / Q000737:chemistry; D007659:Ketones / Q000737:chemistry*; D009243:NAD / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013010:Sonication; D013314:Stress, Mechanical
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D007659:Ketones; D009243:NAD; D000431:Ethanol; D002244:Carbon; D012822:Silicon Dioxide; D000429:Alcohol Oxidoreductases; D005976:Glutaral