[Interaction between mixed-lineage leukemia and Menin proteins].
The study was aimed to investigate the subcellular localization of mixed-lineage leukemia (MLL) and Menin proteins, and to explore the interaction between these two proteins. The recombinant eukaryotic cell expression vectors of pcDNA3.1-myc-MLL and pCMV-flag-Menin were constructed respectively, and transfected into the HEK293T cells. Immunofluorescence technique was used to observe the subcellular localization of the two proteins. Co-immunoprecipitation and Western blot methods were applied to evaluate the expression and interaction of the two proteins. The results showed that MLL and Menin proteins could be co-localized in cell nuclei, and the study of binding in vivo revealed that MLL protein could be detected in the immunoprecipitation complex of anti-FLAG, while Menin proteins could also be found in the immunoprecipitation complex of anti-MYC. It is concluded that MLL and Menin proteins co-localized in cell nuclei have same location and the interaction exists between MLL and Menin proteins.
Liu
Lin
L
Department of Hematology and Oncology, Shanghai Children Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Li
Ben-Shang
BS
Ye
Qi-Dong
QD
He
Ying-Yi
YY
Tang
Jing-Yan
JY
D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005822:Genetic Vectors; D057809:HEK293 Cells; D018398:Homeodomain Proteins; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D025941:Protein Interaction Mapping; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014162:Transfection
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C105373:MEN1 protein, human; D011518:Proto-Oncogene Proteins; D051788:Myeloid-Lymphoid Leukemia Protein