Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB.
The Neisseria meningitidis outer membrane protein PorB was expressed in Escherichia coli and purified from inclusion bodies by denaturation in urea followed by refolding in buffered LDAO on a size-exclusion column. PorB has been crystallized in three different crystal forms: C222, R32 and P6(3). The C222 crystal form may contain either one or two PorB monomers in the asymmetric unit, while both the R32 and P6(3) crystal forms contained one PorB monomer in the asymmetric unit. Of the three, the P6(3) crystal form had the best diffraction quality, yielding data extending to 2.3 A resolution.
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232-6600, USA.
Mikio
M
Tanabe
Tina M
TM
Iverson
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D018272:Porins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C057699:porin protein, Neisseria