Preparation of a glucan from the roots of Rubus crataegifolius Bge. and its immunological activity.
A water-soluble glucan (RCP-1) was prepared from the roots of Rubus crataegifolius Bge. by extraction with hot-water, deproteination by Sevag reagent, alpha-amylase treatment and ultrafiltration. RCP-1 consisted of only glucose, and its molecular weight was determined to be approximately 7KD by high performance gel permeation chromatography (HPGPC). Fourier transform infra-red spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), methylation and periodate oxidation analyses indicated that RCP-1 was an alpha-d-glucan. Its main chains were composed of (1-->4)- and (1-->6)-linked alpha-glucopyranosyls, and side chains were single alpha-glucopyranosyl residues attached to the O-6 of glucosyls in the main chains. RCP-1 could increase both cytotoxic activity against B16 melanoma cells and the production of nitric oxide (NO) of macrophages in vitro. Furthermore, in vivo bioassay tests indicated that RCP-1 could remarkably enhance T and B lymphocyte proliferations, augment the phagocytosis of macrophages and increase the tumour necrosis factor-alpha (TNF-alpha) levels in serum.
Ni
Weihua
W
School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Zhang
Xu
X
Bi
Hongtao
H
Iteku
Jeff
J
Ji
Li
L
Sun
Chengxin
C
Fang
Jinbo
J
Tai
Guihua
G
Zhou
Yifa
Y
Zhao
Jimin
J
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D006801:Humans; D007167:Immunotherapy; D008214:Lymphocytes / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000970:Antineoplastic Agents; D005936:Glucans; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D005947:Glucose