gamma-radiation-induced gammaH2AX formation occurs preferentially in actively transcribing euchromatic loci.
The central dogma in radiation biology is that nuclear DNA is the critical target with respect to radiosensitivity. In accordance with the theoretical expectations, and in the absence of a conclusive model, the general consensus in the field has been to view chromatin as a homogeneous template for DNA damage and repair. This paradigm has been called into question by recent findings indicating a disparity in gamma-irradiation-induced gammaH2AX foci formation in euchromatin and heterochromatin. Here, we have extended those studies and provide evidence that gammaH2AX foci form preferentially in actively transcribing euchromatin following gamma-irradiation.
Vasireddy
Raja S
RS
Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, Melbourne, VIC, Australia.
Karagiannis
Tom C
TC
El-Osta
Assam
A
D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D044127:Epigenesis, Genetic / Q000528:radiation effects*; D022041:Euchromatin / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D014158:Transcription, Genetic / Q000528:radiation effects*
D022041:Euchromatin; C468783:H2AX protein, human; D006657:Histones; D004247:DNA