THE EXTRACTION OF THE CELL CONTENT OF MICRO-ORGANISMS BY PERCUSSION OF THE FROZEN CELLS.
A method is described for rapidly obtaining fresh extracts of microorganisms by percussion of the frozen cells. Filtrates giving the biuret reaction and yielding heavy precipitates upon the addition of acetic acid were obtained from the washed cells of cultures of yeast, hemolytic streptococcus, pneumococcus, B. coli, B. xerosis, B. dyphtheriae and the bacillus of bovine tuberculosis.
Department of Biochemistry and the Department of Bacteriology and Immunology, Vanderbilt University School of Medicine, Nashville.
J M
JM
Johlin
R C
RC
Avery