Diallyl sulfide inhibits murine WEHI-3 leukemia cells in BALB/c mice in vitro and in vivo.
It is well documented that enhanced garlic (Allium sativum) consumption leads to decrease in the cancer incidences. Diallyl sulfide (DAS), one of the components of garlic, induces cytotoxicity and apoptosis in many cancer cell lines. The present studies are focused on the in vivo effects of DAS on leukemia WEHI-3 cells in the BALB/c mice. We examined the effects of DAS on the cytotoxicity of WEHI-3 cells and results indicated that DAS decreased the percentage of viable WEHI-3 cells and these effects are dose-dependent. We examined the effects of DAS on WEHI-3 in vivo and the results indicated that DAS decreased the percentage of Mac-3 and CD11b, indicating that the differentiation of the precursor of macrophage cells was inhibited. DAS stimulated the percentage of CD3 and CD19, indicating that the differentiation of the precursor of T and B cells promoted. The weights of liver and spleen indicated that DAS decreased the weight of these organs after being compared to the control groups. One of the major characteristic of WEHI-3 leukemia is the enlarged spleen in murine after intraperitoneal (i.p.) injection of WEHI-3 cells. In conclusion, DAS affects WEHI-3 cells both in vitro and in vivo.
Yu
Fu-Shun
FS
School of Dental Hygiene, China Medical University, Taichung, Taiwan, Republic of China.
Wu
Chih-Chung
CC
Chen
Chi-Tsai
CT
Huang
Shang-Pang
SP
Yang
Jai-Sing
JS
Hsu
Yuan-Man
YM
Wu
Ping-Ping
PP
Ip
Siu-Wan
SW
Lin
Jing-Pin
JP
Lin
Jaung-Geng
JG
Chung
Jing-Gung
JG
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D007202:Indicators and Reagents; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D013154:Spleen / Q000473:pathology; D013440:Sulfides / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D000498:Allyl Compounds; D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D007202:Indicators and Reagents; D008565:Membrane Proteins; D013440:Sulfides; D013778:Tetrazolium Salts; D013844:Thiazoles; C038491:allyl sulfide; C022616:thiazolyl blue