Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice.
DNA and recombinant virus vaccines against swine influenza virus (SIV) have been pursued with promising results, but induce poor immunogenicity. This study evaluated the effects of a vaccine regimen in mice including priming with three DNA vaccines expressing soluble HA (sHA), complete HA (tmHA), or sHA fused with three copies murine C3d (sHA-mC3d3) and boosting with recombinant pseudorabies virus expressing HA (rPRV-HA). Immune responses were monitored by ELISA, HI assays, and virus neutralization. Protective efficacy was evaluated by virus isolation from lungs, distribution in tissues, and pathology following challenge with H3N2 SIV. Priming with sHA-mC3d3 and boosting with rPRV-HA induced higher levels of HA-specific antibodies and yielded the most effective protection. This finding implied that priming with a DNA vaccine expressing C3d fused with antigen and boosting with a recombinant vector vaccine is an effective way to induce protective humoral immunity and prevent some infectious diseases.
Li
Guo-Xin
GX
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, China.
Zhou
Yan-Jun
YJ
Yu
Hai
H
Tian
Zhi-Jun
ZJ
Yan
Li-Ping
LP
Zhang
Qiang
Q
Hu
Shou-Ping
SP
Tong
Guang-Zhi
GZ
D000818:Animals; D015933:Complement C3d / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology
D006388:Hemagglutinins; D022421:Pseudorabies Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D015847:Interleukin-4; D015933:Complement C3d; D007371:Interferon-gamma