Glutathione reductase activity correlates with concentration of extracellular matrix degradation products in synovial fluid from patients with joint diseases.
The mechanisms underlying cartilage matrix degradation in joint diseases is not fully understood but reactive oxygen species are implicated as main causative factors. Comparative studies of glutathione reductase (GR) activity in synovial fluid from patients with rheumatoid arthritis (RA), reactive arthritis (ReA) and osteoarthritis (OA) as well as correlations between GR activity and concentration of the major cartilage components in synovial fluid are presented in this study. We found significantly higher activity of GR in RA (about three-fold) and ReA (about two-fold) than in OA. In RA and ReA patients, GR activity in synovial fluid correlates negatively with the concentrations of collagen and degradation products of sulfated glycosaminoglycans. In OA patients the activity of GR was significantly lower than in RA and ReA, which positively correlated with the concentration of collagen and showed a tendency for positive correlation with the degradation products of sulfated glycosaminoglycans. Our results suggest that in RA and ReA patients increased activity of GR does not prevent the increased degradation of collagen and proteoglycans by ROS.
Sredzińska
Krystyna
K
Department of Medical Chemistry, Medical University of Białystok, Białystok, Poland. krystyna@umwb.edu.pl
Galicka
Anna
A
Porowska
Halina
H
Sredziński
Łukasz
Ł
Porowski
Tadeusz
T
Popko
Janusz
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D016918:Arthritis, Reactive / Q000201:enzymology*; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005980:Glutathione Reductase / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology*; D000091402:Prohibitins; D017382:Reactive Oxygen Species / Q000378:metabolism; D013582:Synovial Fluid / Q000378:metabolism*
C000717331:PHB2 protein, human; D000091402:Prohibitins; D017382:Reactive Oxygen Species; D003094:Collagen; D005980:Glutathione Reductase