MG-63 osteoblast-like cells enhance the osteoprotegerin expression of PC-3 prostate cancer cells.
BACKGROUND
Osteoprotegerin (OPG) expression participates in the pathophysiology of osteoblastic metastasis in prostate cancer.


METHODS
We investigated whether the expression of OPG of PC-3 prostate cancer cells grown in 3-D collagen gels is stimulated by co-culture with MG-63 osteoblast-like cells. The expression of Runx2 (Cbfa1) and OPG were assessed by reverse transcription-polymerase chain reaction and Western blot analysis.


RESULTS
OPG and Runx2 were expressed in both PC-3 and MG-63 cells. OPG expression was remarkably enhanced in PC-3 cells grown in co-culture with MG-63 cells in a time-dependent manner. Runx2 expression of PC-3 cells was not altered by their co-culture with MG-63 cells. OPG expression of PC-3 cells was altered neither by insulin-like growth factor I (IGF-1), transforming growth factor beta1 (TGFbeta1), interleukin 6 (IL-6) nor by dexamethasone and zoledronic acid exogenously added to PC-3 cells.


CONCLUSIONS
The enhancement of the OPG expression in PC-3 cells by MG-63 cells is not mediated by IGF-1, IL-6 and TGFbeta1.
Department of Experimental Physiology, Medical School, University of Athens, 75 Micras Asias, Goudi, Athens, 115 27 Greece.
Haralampos
H
Katopodis
Anastassios
A
Philippou
Roxane
R
Tenta
Charles
C
Doillon
Katerina K
KK
Papachroni
Athanasios G
AG
Papavassiliou
Michael
M
Koutsilieris
D001859:Bone Neoplasms / Q000473:pathology / Q000556:secondary; D002450:Cell Communication / Q000502:physiology*; D018920:Coculture Techniques; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D053244:Osteoprotegerin / Q000096:biosynthesis*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D050659:Core Binding Factor Alpha 1 Subunit; D015850:Interleukin-6; D053244:Osteoprotegerin; C493709:RUNX2 protein, human; C506030:TNFRSF11B protein, human; D053773:Transforming Growth Factor beta1; D007334:Insulin-Like Growth Factor I