[Phylogenetic analysis and PCR-restriction fragment length polymophism identification of Salmonella based on grOEL gene sequence].
OBJECTIVE
To investigate the value of grOEL gene sequence in phylogenetic analysis and typing of Salmonella.


METHODS
The grOEL gene was amplified by PCR, sequenced and analyzed using Bioedit and DNAstar software. The Salmonella strains were identified using PCR-restriction fragment length polymophism (PCR-RFLP).


RESULTS
The conservative and variable regions of grOEL gene of Salmonella serogroup were separately distributed and most of the small mutant regions distributed intermittently among the conservative regions. The phylogenetic tree of Salmonella based on the nucleotides differed from that generated based on the amino acid sequence. O8, O9 and O10 had the closest consanguinity, and 5 patterns were identified by PCR-RFLP.


CONCLUSIONS
The grOEL gene can be used as a genetic marker for phylogenetic analysis of Salmonella and also as a target sequence for Salmonella typing identification.
Department of Pathogenic Biology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Yu-shan
YS
Hu
Jun-hua
JH
Liu
Xing-lin
XL
Pang
Shou-yi
SY
Chen
Xiao-guang
XG
Chen
D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018834:Chaperonin 60; D004269:DNA, Bacterial