A novel and an effective analytical approach for the LC-MS determination of ethyl glucuronide and ethyl sulfate in urine.
An alternative liquid chromatography-mass spectrometry (LC-MS) method based on no discharge (ND) atmospheric pressure chemical ionization (APCI) was developed for the simultaneous determination of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine in negative ion conditions. Abundant [M-H](-) species of EtG and EtS were obtained, allowing to reach limits of quantification (0.1 microg/ml for both analytes), accuracy, and precision comparable to those proposed in the literature. Additionally, the LC-ND-APCI-MS method proved to be reliable, requiring little maintenance even when high throughput analyses (i.e., 6,000 samples per year) were required.
Favretto
Donata
D
Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, Padua, Italy.
Nalesso
Alessandro
A
Frison
Giampietro
G
Viel
Guido
G
Traldi
Pietro
P
Ferrara
Santo Davide
SD
D001274:Atmospheric Pressure; D015415:Biomarkers / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D053593:Forensic Toxicology; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013463:Sulfuric Acid Esters / Q000652:urine*
D015415:Biomarkers; D005965:Glucuronates; D013463:Sulfuric Acid Esters; C093924:ethyl glucuronide; C011612:diethyl sulfate