Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp.
BACKGROUND
Mesenchymal stem cells (MSCs) have been used for clinical application in tissue engineering and regenerative medicine (TERM). To date, the most common source of MSCs has been bone marrow. However, the bone marrow aspirate is an invasive and painful procedure for the donor. Thus, the identification and characterization of alternative sources of MSCs are of great importance. This study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) compared with dental pulp stem cells (DPSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs).


METHODS
We have compared "stemness" such as the proliferation rate and the expression of stem cell marker of DPSCs, SHED, and BMMSCs. In addition, gene expression profile of DPSCs and SHED were analyzed by using DNA microarray.


RESULTS
All cells isolated from the three sources exhibited MSC characteristics including a fibroblastic morphology, and the expression of mesenchymal stem-cell markers. The proliferation rate of SHED was significantly higher than that of DPSCs and BMMSCs (P < 0.05). The comparison of the gene expression profiles indicated 4386 genes with a changed expression between DPSCs and SHED by 2.0-fold or more. Higher expression in SHED was observed for genes that participate in pathways related to cell proliferation and extracellular matrix, including several cytokines such as fibroblast growth factor and tumor growth factor beta.


CONCLUSIONS
Because of its advantages of a higher proliferation capability, abundant cell supply, and painless stem cell collection with minimal invasion, SHED could be a desirable option as a cell source for potential therapeutic applications.
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
Sayaka
S
Nakamura
Yoichi
Y
Yamada
Wataru
W
Katagiri
Takayuki
T
Sugito
Kenji
K
Ito
Minoru
M
Ueda
D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D055395:Bone Morphogenetic Protein 1 / Q000235:genetics; D049109:Cell Proliferation; D055513:Connective Tissue Growth Factor / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D016220:Fibroblast Growth Factor 1 / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020932:Nerve Growth Factor / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014080:Tooth Exfoliation; D014094:Tooth, Deciduous / Q000166:cytology*; D053781:Transforming Growth Factor beta2 / Q000235:genetics; D053782:Transforming Growth Factor beta3 / Q000235:genetics
D000954:Antigens, Surface; C526317:CCN2 protein, human; D016326:Extracellular Matrix Proteins; D005819:Genetic Markers; D053583:Interleukin-1beta; C525591:STRO-1 antigen, human; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D055513:Connective Tissue Growth Factor; D020932:Nerve Growth Factor; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1