Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR.
This study was designed to develop a multiplex PCR method with five specific primer pairs for the detection of Salmonella spp., Salmonella subspecies I, Salmonella enterica serovars Typhimurium, Typhi and Enteritidis. A multiplex PCR was constructed with five primer pairs for the detection of Salmonella and pathogenic Salmonella serovars, including a specific primer pair for Salmonella Typhi, based on the sequence comparison between genomic DNA sequences of 12 Salmonella strains. Each primer pair was specifically targeted to Salmonella spp., Salmonella subspecies I, Salmonella Typhimurium, Typhi and Enteritidis. This multiplex PCR was evaluated with various DNAs of Salmonella serovars that yielded high specificity for amplifying the expected PCR products of Salmonella serovars. Using this primer pair, a set of multiplex PCR was performed for the rapid identification of salmonellae and major pathogenic Salmonella serovars. Although this multiplex PCR method will need to be evaluated for a wide range of Salmonella serovars among multilaboratories, it should be useful for identifying clinically significant strains of Salmonella serovars rapidly and accurately without the need for serological testing.
Institute of Life Sciences and Resources, Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.
Si Hong
SH
Park
Hyun Joong
HJ
Kim
Woo Hee
WH
Cho
Jae Hwan
JH
Kim
Mi Hwa
MH
Oh
Sung Hun
SH
Kim
Bok Kwon
BK
Lee
Steven C
SC
Ricke
Hae Yeong
HY
Kim
D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012480:Salmonella Infections / Q000382:microbiology; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial