New insights on O2 uptake mechanisms in two-phase partitioning bioreactors.
Numerous reports show that both transfer and uptake of poorly-water soluble substrates are significantly enhanced in two-phase partitioning bioreactors (TPPBs). A number of hypotheses have been put forward to explain these enhancements and among them, the occurrence of direct substrate or oxygen uptake from the vector/water interface has been suggested. The objective of this paper was to quantify the direct oxygen uptake from the vector/water interface in a culture of Pseudomonas putida, performed in a stirred tank reactor, using glucose as substrate and silicone oil as vector. Despite of a sufficient dissolved O(2) concentration in the vector phase (17 mg l(-1)) and a significant vector surface area (4,000 m(-1)) no significant direct O(2) uptake from the vector/water interface was observed, compared to O(2) uptake from the aqueous phase. From these results it was concluded that, direct O(2) or substrate uptake from the vector/water interface might not be significant in TPPBs.
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav), Apdo Postal 14-740, Mexico, DF 07360, Mexico.
Guillermo
G
Quijano
Alberto
A
Ordaz
Raúl
R
Muñoz
Frédéric
F
Thalasso
D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D016958:Pseudomonas putida / Q000378:metabolism*
D010100:Oxygen