Depth-resolved microscopy of cortical hemodynamics with optical coherence tomography.
We describe depth-resolved microscopy of cortical hemodynamics with high-speed spectral/Fourier domain optical coherence tomography (OCT). Stimulus-evoked changes in blood vessel diameter, flow, and total hemoglobin were measured in the rat somatosensory cortex. The results show OCT measurements of hemodynamic changes during functional activation and represent an important step toward understanding functional hyperemia at the microscopic level.
Photon Migration Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging,Massachusetts General Hospital/Harvard Medical School, Charlestown, Massachusetts 02129, USA.
Vivek J
VJ
Srinivasan
Sava
S
Sakadzić
Iwona
I
Gorczynska
Svetlana
S
Ruvinskaya
Weicheng
W
Wu
James G
JG
Fujimoto
David A
DA
Boas
D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000502:physiology; D001931:Brain Mapping / Q000379:methods*; D002560:Cerebrovascular Circulation / Q000502:physiology; D005583:Fourier Analysis; D006439:Hemodynamics; D006940:Hyperemia / Q000473:pathology; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional; D051381:Rats; D013003:Somatosensory Cortex / Q000033:anatomy & histology* / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods