Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in swine muscle by liquid chromatography-tandem mass spectrometry with immunoaffinity chromatography clean-up.
A rapid and sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method to quantify thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) in swine muscle is described. An immunoaffinity chromatography (IAC) column based on polyclonal antibodies and protein A-sepharose CL 4B was used to clean-up extracted samples. IAC optimized conditions were found that allowed the IAC to be reused for selective binding of TAP, FF, and FFA. The dynamic column capacity was more than 512ng/mL of gel after being used for 15 cycles. From fortified swine muscle samples at levels of 0.4-50ng/g, the average recoveries were 85.2-98.9% with intra- and inter-day variations less than 9.8% and 12.4%, respectively. The limit of quantitation ranged from 0.4 to 4.0microg/kg.
Department of Veterinary Pharmacology and Toxicology, China Agricultural University, Beijing, People's Republic of China.
Pengjie
P
Luo
Xia
X
Chen
Chunlai
C
Liang
Hua
H
Kuang
Liming
L
Lu
Zhigang
Z
Jiang
Zhanhui
Z
Wang
Cun
C
Li
Suxia
S
Zhang
Jianzhong
J
Shen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000276:immunology; D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007163:Immunosorbent Techniques; D016014:Linear Models; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D000900:Anti-Bacterial Agents; D000906:Antibodies; C083312:florfenicol amine; C035534:florfenicol; D013839:Thiamphenicol