Identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks.
In this study, a novel avian beta-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20 degrees C to 100 degrees C) and pH values (range, 3 to 12).
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR China.
Deying
D
Ma
Ruiqin
R
Wang
Wenyan
W
Liao
Zongxi
Z
Han
Shengwang
S
Liu
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D004372:Ducks; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010179:Pancreas; D010802:Phylogeny; D055550:Protein Stability; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D023083:beta-Defensins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D023083:beta-Defensins