A straightforward method for the colorimetric detection of endogenous biological cyanide.
Corrin-based chemosensors allow the rapid and selective colorimetric detection of endogenous biological cyanide. The color change from orange to violet can be easily observed with the "naked eye" (Deltalambda(max) = 51 nm). The methodology works directly in the biological matrix without time-consuming sample pretreatment and the use of special reaction conditions. It was possible to study the enzymatic release of cyanide from its biological precursor linamarin with diffuse reflectance UV-visible (DRUV-vis) spectroscopy on a freshly prepared biological surface. These experiments were accompanied by stopped-flow kinetic measurements under homogeneous conditions. Detection in the biological sample is based on the selective coordination of cyanide to the metal-based chemosensor as proven by UV-vis and (1)H NMR spectroscopy. Examples of applications during food manufacturing are given.
Institute of Inorganic Chemistry, University of Zürich, Switzerland.
Christine
C
Männel-Croisé
Benjamin
B
Probst
Felix
F
Zelder
D045728:Corrinoids / Q000737:chemistry*; D003486:Cyanides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D002365:Manihot / Q000737:chemistry*; D009570:Nitriles / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D001617:beta-Glucosidase / Q000378:metabolism*
D045728:Corrinoids; D003486:Cyanides; D009570:Nitriles; C473700:corrin; C021514:cyanogenic beta-glucosidase; D001617:beta-Glucosidase; C005091:linamarin