[SNPs detection of adiponectin gene and its relationship with carcass and meat quality traits in Qinchuan cattle].
Four hundred and five Qinchuan cattle at the age of 24 months were used to detect SNPs of adiponectin gene by PCR-SSCP and sequencing technology and to analyze the correlation of SNPs with carcass and meat quality traits using the general linear model (GLM) in SPSS program. Five genotypes (AA, AB, BB, CC, CD) were detected,with one G-->C mutation at 64 bp in exon2 of adiponectin in ABBB genotypes and one C-->T mutation at 50 bp in exon3 of adiponectin in CD genotype. G-->C mutation resulted glutamic acid (GGA) into glutamine (GCA) and C-->T mutation resulted serine (TCA) into leucine (TTA). Statistical analysis revealed that Qinchuan cattle with AA genotype was higher than BB genotype in slaughter weight, back fat thickness, carcass weight, loin muscle area (P < 0.05). The crural girth of AA genotype was significantly higher than AB and BB genotypes (P < 0.01). Qinchuan cattle with CD genotype was higher than CC genotype in slaughter weight, subcutaneous fat thickness, back fat thickness, crural girth, and tenderness (P < 0.05). Adiponectin gene was proved to be closely related to carcass and meat quality traits (P < 0.05), which can be used as a candidate molecular marker for production of high-grade meat in Qinchuan beef cattle.
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China. yangyanjie817@163.com
Yan-Jie
YJ
Yang
D052242:Adiponectin / Q000235:genetics*; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D008460:Meat / Q000592:standards*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics
D052242:Adiponectin