Antibacterial compounds from mushrooms II: lanostane triterpenoids and an ergostane steroid with activity against Bacillus cereus isolated from Fomitopsis pinicola.
Anti- Bacillus cereus bioassay-guided fractionation of a crude extract of the American mushroom, Fomitopsis pinicola, was performed using thin-layer chromatography, Sephadex LH-20 column chromatography, and preparative-scale HPLC. Five lanostane triterpenoids (1-5) and one ergostane steroid (6) were isolated and identified. Compound 1 is a new lanostane triterpenoid, and its structure was determined using 1D and 2D NMR experiments, HR-MS, and physical data. Each of the purified compounds (1-6) was tested for antibacterial activity against B. cereus using standard MIC assays. Compounds 1-6 had MIC values of 32, 16, 32, 32, 128, and 64 microg/mL, respectively.
Department of Chemistry, University of Wisconsin - La Crosse, La Crosse, Wisconsin 54601, USA.
Xue-Ting
XT
Liu
Abby L
AL
Winkler
William R
WR
Schwan
Thomas J
TJ
Volk
Marc
M
Rott
Aaron
A
Monte
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D000898:Antibiosis; D001409:Bacillus cereus / Q000187:drug effects* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D055453:Coriolaceae / Q000737:chemistry*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D014315:Triterpenes; C015190:ergostane; D004875:Ergosterol