Next-generation 9216-microwell cell arrays for high-content screening microscopy.
Reverse transfection on cell arrays is a high-throughput method for the parallel transfection of mammalian cells for use in high-content screening light microscopy. Here, we present novel 9216-microwell cell arrays which combine the advantages of multiwell plates (physically separated samples) and cell microarrays (high sample density and long-term storage).
Reymann
Jürgen
J
ViroQuant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, Germany.
Beil
Nina
N
Beneke
Jürgen
J
Kaletta
Peter-Paul
PP
Burkert
Klaus
K
Erfle
Holger
H
D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005240:Feasibility Studies; D005780:Gelatin / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D020527:Microscopy, Scanning Probe; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics; D013379:Substrate Specificity; D014025:Titanium / Q000737:chemistry; D014162:Transfection
D020099:Coated Materials, Biocompatible; D034741:RNA, Small Interfering; D005780:Gelatin; D014025:Titanium