Synthesis of an unlocked nucleic acid terpyridine monomer and binding of divalent metal ion in nucleic acid duplexes.
Herein we present the synthesis and thermal stability studies of modified oligonucleotides containing an unlocked nucleic acid (UNA) terpyridine monomer. Incorporation of this monomer into both strands of a DNA duplex allowed reversible thermal stability modulation upon addition or withdrawal of divalent metal ions. A likely explanation of this phenomenon is interstrand complexation between two terpyridine units and a metal ion. This system could be useful in the development of nanoscale devices based on DNA hybridization.
Karlsen
Kasper K
KK
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense, Denmark.
Jensen
Troels B
TB
Wengel
Jesper
J
D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D015032:Zinc / Q000737:chemistry*
D007477:Ions; D009696:Nucleic Acids; D009841:Oligonucleotides; D011725:Pyridines; D003300:Copper; D009532:Nickel; D015032:Zinc