Friend of Prmt1, a novel chromatin target of protein arginine methyltransferases.
We describe the isolation and characterization of Friend of Prmt1 (Fop), a novel chromatin target of protein arginine methyltransferases. Human Fop is encoded by C1orf77, a gene of previously unknown function. We show that Fop is tightly associated with chromatin, and that it is modified by both asymmetric and symmetric arginine methylation in vivo. Furthermore, Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes, including TFF1 (pS2). Fop depletion results in an almost complete block of estradiol-induced promoter occupancy by the estrogen receptor. Our data indicate that Fop recruitment to the promoter is an early critical event in the activation of estradiol-dependent transcription.
Erasmus MC, Department of Cell Biology, Rotterdam, The Netherlands.
Thamar Bryn
TB
van Dijk
Nynke
N
Gillemans
Claudia
C
Stein
Pavlos
P
Fanis
Jeroen
J
Demmers
Mariëtte
M
van de Corput
Jeroen
J
Essers
Frank
F
Grosveld
Uta-Maria
UM
Bauer
Sjaak
S
Philipsen
D000818:Animals; D001120:Arginine / Q000378:metabolism; D019921:Biotinylation; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D008745:Methylation; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011496:Protein Methyltransferases / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012097:Repressor Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014157:Transcription Factors; D015533:Transcriptional Activation
C541075:CHTOP protein, human; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D004958:Estradiol; D001120:Arginine; D011496:Protein Methyltransferases; C099988:PRMT1 protein, human; C546338:Prmt1 protein, mouse; C498518:Prmt5 protein, mouse; D011484:Protein-Arginine N-Methyltransferases