Comparison of microscopic methods for evaluating platelet adhesion to biomaterial surfaces.
Microscopic methods usable for sample surface imaging and subsequent qualitative and quantitative evaluation of platelet adhesion to the surface of the biomaterial studied were compared. It was shown, making use of the samples of medical steel (AISI 316L), that such tools as surface imaging with scanning electron microscopy (SEM), glutaraldehyde induced fluorescence technique (GIFT) and metallurgical microscopy (MM) are equivalent in evaluating surface platelet adhesion. The importance of biological variability of blood samples for a proper result assessment and the necessity of using internal standards were also considered.
Okrój
Wiesława
W
Institute of Materials Science and the Centre of Excellence NANODIAM, Technical University of Łódź, and the Centre of Advanced Technology BioTechMed, Łódź, Poland.
Walkowiak-Przybyło
Magdalena
M
Rośniak-Bak
Kinga
K
Klimek
Leszek
L
Walkowiak
Bogdan
B
D001672:Biocompatible Materials / Q000378:metabolism* / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008853:Microscopy / Q000379:methods*; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D013232:Steel / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects
D001672:Biocompatible Materials; D013232:Steel