[Effect of jieze no. 1 gel on vaginal levels of lactobacillus, glycogen and lactoferrin in mice].
OBJECTIVE
To evaluate the effect of Jieze No. 1 Gel (JZ1) on the inner environment of vagina through observing its influences on vaginal levels of lactobacillus, glycogen and lactoferrin in mice.


METHODS
One hundred female Kunming mice were randomized into 5 groups, the blank control (BC) group, the blank gel (BG) group, and the three JZ1 treated groups. They were treated via vagina with saline, matrix gel, high (2 mg/microL), middle (1 mg/microL) and low (0.5 mg/microL) dose JZ1 respectively in volume of 20 microL, 72 h after being subcutaneously injected with physiological estradiol benzoate (E2) 2 microg. The 24 h and 72 h living lactobacillus in vaginal lavage fluid cultures were counted, the content of glycogen and the expression of lactoferrin in vaginal tissue were measured.


RESULTS
No significant difference was found between the BC group and the three JZ1 treated groups in terms of lactobacillus-CFU, glycogen content and lactoferrin expression, and these indices detected at 24 h were not different to those detected at 72 h in the groups treated with various doses of JZ1 respectively (P > 0.05).


CONCLUSIONS
Once application of JZ1 shows no effect on levels of lactobacillus, glycogen, and lactoferrin expression in the vagina of mice.
Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. chenz@tjh.tjmu.edu
Zhuo
Z
Chen
Wen
W
Gao
Yong-Gui
YG
Ma
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005782:Gels; D006003:Glycogen / Q000378:metabolism*; D007778:Lactobacillus / Q000302:isolation & purification*; D007781:Lactoferrin / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D014621:Vagina / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology
D004365:Drugs, Chinese Herbal; D005782:Gels; D006003:Glycogen; D007781:Lactoferrin