[Nandeshi: a powerful inhibitor of human acrosin activity].
OBJECTIVE
To evaluate the inhibitory effect of Nandeshi, an acrosin inhibitor, on human acrosin activity.


METHODS
We collected sperm samples from 10 healthy fertile men and cultured them with Nandeshi at 30 degrees C for 5 minutes at the concentrations of 0. 100, 0.120, 0.144, 0.173, 0.207, 0.249, 0.299, 0.358 and 0.430 mmol/L, with the controls treated with a well-known acrosin inhibitor N-alpha-p-tosyl-L-lysine chloromethylketone (TLCK) at 150.0, 189.8, 213.6, 240.3, 270.3, 304.1 and 342.1 mmol/L. Then we determined the residual activity of human acrosin by improved Kennedy assay.


RESULTS
The residual activity of acrosin was negatively correlated with the Nandeshi concentration, and Nandeshi exhibited an inhibition rate about 800 times that of TLCK.


CONCLUSIONS
Nandeshi has a powerful inhibitory effect on human acrosin, and improved Kennedy assay is a simple, practical and highly sensitive technique for the detection of human acrosin activity.
Song
Gui-Hong
GH
Research Center of Reproductive Medicine, Changhai Hospital, 2. School of Pharmacology, The Second Military, Shanghai 200433, China.
Zhang
Jue
J
Zhang
Xiao-Meng
XM
Ning
Wei-Wei
WW
Ji
Ya-Zhong
YZ
Hui
Ning
N
Zhao
Ya-Nan
YN
Zhou
You-Jun
YJ
Zhu
Ju
J
Lü
Jia-Guo
JG
D000176:Acrosin / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000187:drug effects; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D003271:Contraceptive Agents, Female; D004791:Enzyme Inhibitors; D014107:Tosyllysine Chloromethyl Ketone; D000176:Acrosin