Identification of prenyl flavonoid glycosides and phenolic acids in Epimedium koreanum Nakai by Q-TOF-MS combined with selective enrichment on "click oligo (ethylene glycol)" column.
Prenyl flavonoid glycosides and phenolic acids are constituents of the medicinal plant Epimedium koreanum Nakai (EK). An efficient method was developed to enrich these compounds and identify them, using ultra performance liquid chromatography combined with Q-TOF-MS, and a "click oligo (ethylene glycol)" (Click OEG) column. Using this method, 51 prenyl flavonoid glycosides and 18 phenolic acids were identified or tentatively identified. Of these, 11 prenyl flavonoid glycosides and 4 phenolic acids were new compounds, and 7 phenolic acids were newly identified in EK. Therefore, MS combined with selective enrichment provided a powerful means for analyzing prenyl flavonoid glycosides and phenolic acids.
Key Lab of Separation Science for Analytical Chemistry Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Yaqin
Y
Wang
Zhimou
Z
Guo
Yu
Y
Jin
Xiuli
X
Zhang
Li
L
Wang
Xingya
X
Xue
Xinmiao
X
Liang
D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D034181:Epimedium; D019855:Ethylene Glycol; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis* / Q000737:chemistry; D035261:Plant Components, Aerial; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D062385:Hydroxybenzoates; D019855:Ethylene Glycol; C017616:phenolic acid