TRAP plays a role in stress response in Staphylococcus aureus.
Staphylococci are common pathogens of implant-related infections. RIP is a heptapeptide (YSPWTNF-NH2 ) that was shown to be very effective in preventing and treating antibiotic-resistant staphylococcal infections, in healing polymicrobial wounds, and in enhancing the effect of commonly used antibiotics. How the peptide negatively affects the survival of the bacteria in the host is not yet known. In staphylococci, RIP was shown to suppress toxin production by inhibiting the expression of agr and production of RNAIII. RIP was also shown to suppress the phosphorylation of TRAP (target of RNAIII-activating peptide), whose function was not clear. Here we show that mutant S. aureus TRAP- cells were more sensitive to oxidative stress and had higher rates of spontaneous and adaptive (agr) mutations. Furthermore, recombinant TRAP protected DNA from oxidative damage caused by hydroxyl radicals. Put together, these results suggest that TRAP is involved in DNA protection from stress. RIP may thus suppress pathogenesis through multiple independent molecular mechanisms involving both suppression of virulence and suppression of stress response.
Kiran
Madanahally D
MD
Department of Biological Sciences, Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA.
Balaban
Naomi
N
D048868:Adaptor Proteins, Signal Transducing; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D004249:DNA Damage; D015964:Gene Expression Regulation, Bacterial; D017665:Hydroxyl Radical / Q000378:metabolism; D009154:Mutation; D009842:Oligopeptides / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D014774:Virulence
D048868:Adaptor Proteins, Signal Transducing; C095698:Agr protein, Staphylococcus aureus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D009842:Oligopeptides; D010750:Phosphoproteins; C428447:RAP protein, Staphylococcus aureus; D011994:Recombinant Proteins; C428446:TRAP protein, Staphylococcus; D015534:Trans-Activators; D017665:Hydroxyl Radical