Poly(ADP-ribose) polymerase-1 (PARP-1) longer alleles spanning the promoter region may confer protection to bilateral Meniere's disease.
CONCLUSIONS
The longer alleles (CA)17-20 of the promoter region of PARP-1 gene may confer some protection against bilateral Meniere's disease (BMD).


OBJECTIVE
To analyze microsatellite (CA)(n) polymorphisms in the promoter region of PARP-1 gene and seek out risk and protective variants for BMD.


METHODS
Eighty patients from two ethnically defined groups with definite BMD, according to the diagnostic scale of the American Academy of Otolaryngology Head and Neck Surgery, were compared with a group of 371 normal controls from the same origin in a prospective multicenter study. We developed a specific amplification protocol to determine the PARP1-promotor CA microsatellite polymorphisms.


RESULTS
We found that the longer alleles (CA)17-20 had a very low frequency in BMD (2/160, 1.3%, OR=7.33 (1.77-30.37, 95% CI), corrected p=0.012), suggesting that it may confer some protection against BMD.
Lopez-Escamez
Jose A
JA
Otology & Neurotology Group, Department of Otolaryngology, Hospital de Poniente de Almería, Ctra. de Almerimar s/n, El Ejido, Almería. jalopeze@cajamar.es
Moreno
Antonia
A
Bernal
Monica
M
Perez-Garrigues
Herminio
H
Santos-Perez
Sofia
S
Soto-Varela
Andres
A
Aran
Ismael
I
Fernandez-Sanfrancisco
Omar
O
Lopez-Nevot
Alicia
A
Lopez-Nevot
Miguel A
MA
D000483:Alleles; D022981:Allelic Imbalance / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008575:Meniere Disease / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011446:Prospective Studies; D012016:Reference Values; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases