The role of the intravascular microenvironment in spontaneous metastasis development.
Metastasis is primarily responsible for the morbidity and mortality of cancer. Improved therapeutic outcomes and prognosis depend on improved understanding of mechanisms regulating the establishment of early metastasis. In this study, use of green fluorescent protein (GFP)-expressing PC-3 orthotopic model of human prostate cancer and two complementary fluorescence in vivo imaging systems (Olympus OV100 and VisEn FMT) allowed for the first time real-time characterization of cancer cell-endothelium interactions during spontaneous metastatic colonization of the liver and lung in live mice. We observed that prior to the detection of extra-vascular metastases, GFP-expressing PC-3 cancer cells resided initially inside the blood vessels of the liver and the lung, where they proliferated and expressed Ki-67 and exhibited matrix metalloprotenases (MMP) activity. Thus, the intravascular cancer cells produced their own microenvironment, where they could continue to proliferate. Extravasation occurred earlier in the lung than in the liver. Our results demonstrate that the intravascular microenvironment is a critical staging area for the development of metastasis that later can invade the parenchyma. Intravascular tumor cells may represent a therapeutic target to inhibit the development of extravascular metastases. Therefore, this imageable model of intravascular metastasis may be used for evaluation of novel anti-metastatic agents.
Department of Pathology, Cellular and Radiation Oncology, The University of Chicago, Illinois 60637, USA.
Qingbei
Q
Zhang
Meng
M
Yang
Jikun
J
Shen
Lynnette M
LM
Gerhold
Robert M
RM
Hoffman
H Rosie
HR
Xing
D000818:Animals; D045744:Cell Line, Tumor; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000473:pathology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology / Q000556:secondary; D008168:Lung / Q000201:enzymology / Q000473:pathology; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology / Q000556:secondary; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000473:pathology* / Q000503:physiopathology; D009368:Neoplasm Transplantation / Q000379:methods / Q000662:veterinary; D009369:Neoplasms / Q000098:blood supply / Q000401:mortality; D011471:Prostatic Neoplasms / Q000098:blood supply* / Q000201:enzymology / Q000473:pathology / Q000601:surgery
D049452:Green Fluorescent Proteins; D020782:Matrix Metalloproteinases