[Investigation of uninterpretative HLA typing in 311 umbilical cord blood samples].
The aim of this study was to investigate the factors which affect HLA typing in 311 umbilical cord blood (UCB) samples. The HLA low resolution typing of UCB samples with misinterpreted HLA types from 311 UCB samples analyzed by PCR-SSO and PCR-SSP was performed. 7 samples difficult to determine their HLA genotype were sequenced directly and the reason leading to misinterpret HLA typing was analyzed. The results indicated that 99.4% of misinterpreted samples resulted from the restriction of HLA typing method itself and 0.6% of misinterpreted samples were suspected to be contaminated with maternal blood in UCB. It is concluded that HLA typing is mainly affected by the shortcomings of oligonucleotide probe design for PCR-SSO and lack of allele specific primers of PCR-SSP.
Union Stem Cell & Gene Engineering Co., Ltd., Tianjin Cord Blood Bank, Tianjin 300384, China.
Jing-Xin
JX
Hong
Xiao-Lan
XL
Liang
Jun-Ling
JL
Han
Qian
Q
Li
Lu-Gui
LG
Qiu
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005312:Fetal Blood / Q000276:immunology*; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D006680:HLA Antigens; D015345:Oligonucleotide Probes