A practical approach to screen for authorised and unauthorised genetically modified plants.
In routine analysis, screening methods based on real-time PCR are most commonly used for the detection of genetically modified (GM) plant material in food and feed. In this paper, it is shown that the combination of five DNA target sequences can be used as a universal screening approach for at least 81 GM plant events authorised or unauthorised for placing on the market and described in publicly available databases. Except for maize event LY038, soybean events DP-305423 and BPS-CV127-9 and cotton event 281-24-236 x 3006-210-23, at least one of the five genetic elements has been inserted in these GM plants and is targeted by this screening approach. For the detection of these sequences, fully validated real-time PCR methods have been selected. A screening table is presented that describes the presence or absence of the target sequences for most of the listed GM plants. These data have been verified either theoretically according to available databases or experimentally using available reference materials. The screening table will be updated regularly by a network of German enforcement laboratories.
Waiblinger
Hans-Ulrich
HU
State Institute for Chemical and Veterinary Analysis of Food Freiburg, Chemisches und Veterinäruntersuchungsamt Freiburg, Bissierstr. 5, 79114 Freiburg, Germany. hans-ulrich.waiblinger@cvuafr.bwl.de
Grohmann
Lutz
L
Mankertz
Joachim
J
Engelbert
Dirk
D
Pietsch
Klaus
K
D003257:Consumer Product Safety; D018556:Crops, Agricultural / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*