Protective effect of Corchorus olitorius leaves on sodium arsenite-induced toxicity in experimental rats.
The present study was undertaken to evaluate the protective effect of aqueous extract of Corchorus olitorius leaves (AECO) against sodium arsenite-induced toxicity in experimental rats. The animals exposed to sodium arsenite at a dose of 10mg/kg body weight p.o. for 10days exhibited a significant inhibition (p<0.01) of hepatic and renal antioxidant enzymes namely superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase and glutathione reductase. In addition, arsenic intoxication significantly decreased (p<0.01) the level of reduced glutathione and increased (p<0.01) the levels of oxidized glutathione and thiobarbituric acid reactive substances in selected tissues. Treatment with AECO at doses of 50 and 100mg/kg body weight p.o. for 15days prior to arsenic intoxication significantly improved hepatic and renal antioxidant markers in a dose dependant manner. AECO treatment also significantly reduced the arsenic-induced DNA fragmentation of hepatic and renal tissues. Histological studies on the ultrastructural changes of liver and kidney supported the protective activity of the AECO. The results concluded that the treatment with AECO prior to arsenic intoxication has significant role in protecting animals from arsenic-induced hepatic and renal toxicity.
Das
Anup K
AK
Department of Pharmaceutical Sciences, Dibrugarh University, Assam, India.
Bag
Sujit
S
Sahu
Ranabir
R
Dua
Tarun K
TK
Sinha
Mohit K
MK
Gangopadhyay
Moumita
M
Zaman
Kamaruz
K
Dewanjee
Saikat
S
D000818:Animals; D018053:Arsenites / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D032381:Corchorus / Q000737:chemistry*; D053938:DNA Fragmentation; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018053:Arsenites; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D017670:Sodium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C017947:sodium arsenite; D011794:Quercetin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione