Conformation and thermostability of oligonucleotide d(GGTTGGTGTGGTTGG) containing thiophosphoryl internucleotide bonds at different positions.
The thrombin-binding aptamer d(GGTTGGTGTGGTTGG) (TBA) is an efficient tool for the inhibition of thrombin function. We have studied conformations and thermodynamic stability of a number of modified TBA oligonucleotides containing thiophosphoryl substitution at different internucleotide sites. Using circular dichroism such modifications were found not to disrupt the antiparallel intramolecular quadruplex specific for TBA. Nevertheless, the presence of a single thiophosphoryl bond between two G-quartet planes led to a significant decrease in the quadruplex thermostability. On the contrary, modifications in each of the loop regions either stabilized an aptamer structure or did not reduce its stability. According to the thrombin time test, the aptamer with thio-modifications in both TT loops (LL11) exhibits the same antithrombin efficiency as the original TBA. This aptamer shows better stability against DNA nuclease compared to that of TBA. We conclude that such thio-modification patterns are very promising for the design of anticoagulation agents.
Institute for Physical-Chemical Medicine of Ministry of Public Health, Moscow, Russian Federation.
Marina
M
Zaitseva
Dmitry
D
Kaluzhny
Anna
A
Shchyolkina
Olga
O
Borisova
Igor
I
Smirnov
Galina
G
Pozmogova
D000925:Anticoagulants / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002942:Circular Dichroism; D015195:Drug Design; D004355:Drug Stability; D054856:G-Quadruplexes / Q000187:drug effects; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013816:Thermodynamics; D013873:Thionucleotides / Q000737:chemistry*
D000925:Anticoagulants; D052157:Aptamers, Nucleotide; D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides; C081676:thrombin aptamer