Down-regulation of Tip60 gene as a potential marker for the malignancy of colorectal cancer.
BACKGROUND
Recently, it was shown that the loss of human Tip60 leads to an accumulation of double-strand DNA breaks and is linked to a growing number of cancer types.


METHODS
Tip60 expression levels were in examined in 38 colorectal cancer samples using a quantitative real-time polymerase chain reaction. Subsequently, clinicopathological data were correlated with the Tip60 expression score.


RESULTS
A down-regulation of the Tip60 gene was observed 5 out of 38 (13%) specimens of primary colorectal cancer. Tip60 down-regulation showed significant correlation with larger tumor size (p=0.0005), poorly differentiated type (p=0.0394), peritoneal dissemination (p=0.0053), distant metastasis (p=0.0394), and higher stage of TNM classification (p=0.0226).


CONCLUSIONS
These results suggested that Tip60 was more frequently down-regulated in advanced colorectal carcinoma.
Sakuraba
Kazuma
K
Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan.
Yasuda
Takashi
T
Sakata
Makiko
M
Kitamura
Yo-hei
YH
Shirahata
Atsushi
A
Goto
Tetsuhiro
T
Mizukami
Hiroki
H
Saito
Mitsuo
M
Ishibashi
Kazuyoshi
K
Kigawa
Gaku
G
Nemoto
Hiroshi
H
Sanada
Yutaka
Y
Hibi
Kenji
K
D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D051548:Histone Acetyltransferases / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D000074443:Lysine Acetyltransferase 5; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051548:Histone Acetyltransferases; C098489:KAT5 protein, human; D000074443:Lysine Acetyltransferase 5