A chemical platform for improved induction of human iPSCs.
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Lin
Tongxiang
T
Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA.
Ambasudhan
Rajesh
R
Yuan
Xu
X
Li
Wenlin
W
Hilcove
Simon
S
Abujarour
Ramzey
R
Lin
Xiangyi
X
Hahm
Heung Sik
HS
Hao
Ergeng
E
Hayek
Alberto
A
Ding
Sheng
S
D001549:Benzamides / Q000494:pharmacology; D002454:Cell Differentiation / Q000235:genetics*; D004149:Dioxoles / Q000494:pharmacology; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D048369:MAP Kinase Kinase 1 / Q000037:antagonists & inhibitors; D011743:Pyrimidines / Q000494:pharmacology; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D013844:Thiazoles / Q000494:pharmacology; D014161:Transduction, Genetic
C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D001549:Benzamides; D004149:Dioxoles; D011743:Pyrimidines; D018125:Receptors, Transforming Growth Factor beta; D013844:Thiazoles; C545214:thiazovivin; C506614:mirdametinib; D004159:Diphenylamine; D048369:MAP Kinase Kinase 1