Enhanced neutrophil expression of annexin-1 in coronary artery disease.
The systemic inflammatory activity in patients with stable coronary artery disease (CAD) is associated with a dysregulated cortisol response. Moreover, an aberrant activation status of neutrophils in CAD has been discussed; and the question of glucocorticoid resistance has been raised. The anti-inflammatory actions of glucocorticoids are mediated by annexin-1 (ANXA1). We investigated the expression of glucocorticoid receptors (GR) and ANXA1, as well as the exogenous effects of ANXA1 on neutrophils in CAD patients and related the data to diurnal salivary cortisol. Salivary cortisol levels were measured in the morning and evening during 3 consecutive days in 30 CAD patients and 30 healthy individuals. The neutrophil expression of GR and ANXA1 was determined by flow cytometry. The effect of exogenous ANXA1 was determined in a neutrophil stimulation assay. The patients showed a flattened diurnal cortisol pattern compared with healthy subjects, involving higher levels in the evening. The neutrophil expression of GR-total and GR-alpha was decreased, whereas the GR-beta expression did not differ compared with controls. The neutrophil expression of ANXA1 was significantly increased in patients. Ex vivo, ANXA1 impaired the leukotriene B(4)-induced neutrophil production of reactive oxygen species in patients but not in controls. Our findings indicate a persistent overactivation of the hypothalamic-pituitary-adrenal axis in CAD patients but do not give any evidence for glucocorticoid resistance, as assessed by the neutrophil expression of GR and ANXA1. The altered neutrophil phenotype in CAD may thus represent a long-term response to disease-related activation.
Särndahl
Eva
E
Department of Biomedicine, School of Health and Medical Sciences, Orebro University, SE-701 82 Orebro, Sweden. eva.sarndahl@oru.se
Bergström
Ida
I
Nijm
Johnny
J
Forslund
Tony
T
Perretti
Mauro
M
Jonasson
Lena
L
D000368:Aged; D000792:Angiography; D017305:Annexin A1 / Q000096:biosynthesis*; D018821:CD18 Antigens / Q000096:biosynthesis; D002940:Circadian Rhythm / Q000502:physiology; D003324:Coronary Artery Disease / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000096:biosynthesis / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007975:Leukotriene B4 / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010641:Phenotype; D017382:Reactive Oxygen Species; D044042:Receptors, Formyl Peptide / Q000096:biosynthesis; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis; D044067:Receptors, Lipoxin / Q000096:biosynthesis; D012463:Saliva / Q000378:metabolism
D017305:Annexin A1; D018821:CD18 Antigens; C105101:FPR2 protein, human; D005938:Glucocorticoids; D017382:Reactive Oxygen Species; D044042:Receptors, Formyl Peptide; D011965:Receptors, Glucocorticoid; D044067:Receptors, Lipoxin; D007975:Leukotriene B4; D006854:Hydrocortisone