Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus.
Recent studies have indicated that Staphylococcus aureus can survive the nitrosative stress (caused by the radical nitric oxide; NO.) mounted by the immune system of the infected host. It does this by expressing a nitric oxide-inducible L-lactate dehydrogenase (Sa-LDH-1). Therefore, if efficient inhibitors of Sa-LDH-1 can be designed then Sa-LDH-1 could be a potential drug target against the pathogen S. aureus. For this purpose, the nitric acid-inducible LDH-1 from S. aureus COL strain has been cloned into the expression vector pET-28a(+) and the protein has been expressed, purified and crystallized. The Sa-LDH-1 crystal diffracted to 2.4 A resolution at a home X-ray source and belonged to space group C2, with unit-cell parameters a = 131.4, b = 74.4, c = 103.2 A, beta = 133.4 degrees .
Dong
Jia Bin
JB
Department of Microbiology, Tianjin Medical University, Tianjin 300070, People's Republic of China.
Liu
Xiang
X
Li
Lan Fen
LF
Wu
Shangwei
S
Su
Xiao Dong
XD
D003460:Crystallization; D018360:Crystallography, X-Ray; D004790:Enzyme Induction; D007527:Isoenzymes / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D009569:Nitric Oxide / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000201:enzymology*
D007527:Isoenzymes; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1