[Small interfering RNA-mediated islet neogenesis associated protein gene silencing inhibits the proliferation of INS-1 islet cells].
OBJECTIVE
To investigate the effect of small interfering RNA (siRNA)-mediated islet neogenesis associated protein (INGAP) gene silencing on the proliferation of islet cells.


METHODS
Different siRNAs targeting INGAP gene were designed and transfected into INS-1 islet cells, and the expression levels of INGAP mRNA and protein following the transfection were detected using RT-PCR, flow cytometry and Western blotting. The proliferation of the transfected INS-1 cells was evaluated using MTT assay.


RESULTS
Compared with those in the irrelevant siRNA, empty vector control, and un-transfected groups, the expression levels of INGAP mRNA and protein in the cells transfected with siRNA6 were reduced significantly. The cell proliferation rate significantly increased after transfection with siRNA6 (P<0.05).


CONCLUSIONS
siRNA targeting INGAP can effectively down-regulate INGAP expression and inhibit the proliferation of INS-1 cells.
Sha
Jian-ping
JP
Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shajianping99@sina.com
Xue
Yao-ming
YM
Chen
Xuan
X
Long
Ke
K
Liang
Hua-cheng
HC
Sang
Dan
D
Mao
Rui-rui
RR
Lin
Zhan
Z
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D007340:Insulinoma / Q000473:pathology; D007515:Islets of Langerhans / Q000473:pathology*; D037181:Lectins, C-Type / Q000235:genetics*; D000073718:Pancreatitis-Associated Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D034741:RNA, Small Interfering; C000612144:Reg3b protein, rat