Respiratory syncytial virus infection of camels (Camelus dromedaries).
This study aimed to investigate the occurrence of respiratory syncytial virus (RSV) infections in camels in Sudan. A total of 272 camel lung specimens showing pneumonia were collected from slaughter houses at four different areas in Sudan, additionally 8 specimens were collected from outbreaks of respiratory infection in camels. Using sandwich ELISA kits for RSV antigen detection 4 out of 280 tested lungs (1.4%) were positive, all were from Central Sudan (Tambool slaughter house). FAT was used to confirm the ELISA positives. Polymerase chain reaction RT/PCR was applied for the detection of RSV genome in camel lungs; 1 out of 4 ELISA positives was positive by RT/PCR. Using indirect ELISA kits 135 out of 495 (27.3%) camel sera showed antibodies to RSV, highest prevalence was observed in Western (33.5%) then Central (31.6%) and Eastern Sudan (23.5%). Based on the manufacturer specified calculations for OD readings, most of positive sera (90/135) were low reactive (1+). This is the first report for the detection of RSV antigen, genome and antibody in camels in Sudan.
Central Veterinary Research Laboratory, P.O. Box 8067, Al Amarat, Khartoum, Sudan. intisarkamil@yahoo.com
K S
KS
Intisar
Y H
YH
Ali
A I
AI
Khalafalla
Mahasin E A
ME
Rahman
A S
AS
Amin
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification; D002162:Camelus / Q000821:virology*; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D008168:Lung / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013397:Sudan / Q000453:epidemiology
D000914:Antibodies, Viral; D000956:Antigens, Viral