The role of polyamines in the regulation of the plasma membrane and the tonoplast proton pumps under salt stress.
Polyamine content (PAs) often changes in response to abiotic stresses. It was shown that the accumulation of PAs decreased in roots treated for 24h with 200 mM NaCl. The role of polyamines (putrescine - PUT, spermidine - SPD and spermine - SPM) in the modification of the plasma membrane(PM) H(+)-ATPase (EC 3.6.3.6) and the vacuolar(V) H(+)-ATPase (EC 3.6.3.14) activities in cucumber roots treated with NaCl was investigated. 24h treatment of seedlings with 50 microM PUT, SPD or SPM lowered the activities of proton pumps in both membranes. The decreased H(+)-ATPase activity in plasma membranes isolated from the PA-treated roots was positively correlated with a lower level of PM-H(+)-ATPase CsHA3 transcript. However, transcript levels of PM-H(+)-ATPase CsHA2 and V-ATPase subunit A and c in roots treated with 50 microM PAs were similar to those in the control. Additionally, treatment of plants with salt markedly increased the activity of the PM- and V-H(+)-ATPases. However, exposure of plants to 20% PEG had no effect on these activities. These data suggest that, under salt stress conditions, the increase in H(+)-ATPase activities is caused mainly by the ionic component of salt stress. It seems that the main role of the PAs in the 24h salt-treated cucumber plants could be a result of their cationic character. The PA levels decreased when concentration of Na(+) increased, so action of PAs contributes to ionic equilibrium. Moreover, the decrease in the concentration of polyamines, which inhibit the PM-H(+)-ATPase and the V-H(+)-ATPase, at least under the studied conditions, seems to be beneficial. Thus, plants can increase salinity tolerance by modifying the biosynthesis of polyamines.
Janicka-Russak
Małgorzata
M
Department of Plant Physiology, Institute of Plant Biology, University of WrocŁaw, Kanonia 6/8, 50-328 WrocŁaw, Poland. russak@biol.uni.wroc.pl
Kabała
Katarzyna
K
Młodzińska
Ewa
E
Kłobus
Grazyna
G
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D018533:Biomass; D015153:Blotting, Western; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018553:Cucumis sativus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions; D013312:Stress, Physiological / Q000187:drug effects*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism*
D011073:Polyamines; D021122:Protein Subunits; D017494:Proton Pumps; D012333:RNA, Messenger; D012996:Solutions; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium