Efficient conjugation of oligonucleotides through aromatic oxime formation.
The present work reports on the preparation of oligonucleotide conjugates via the formation of aromatic oxime linkage. The conjugation consists in the reaction between the oligonucleotide derivatized at 5'-extremity with a benzaldehyde moiety and an aminooxy reporter group. The conjugation was found highly efficient and was extended for the conjugation of phosphorothioate oligonucleotide. In addition, the stability of the so-formed oxime conjugate was investigated.
Murat
Pierre
P
Département de Chimie Moléculaire, UMR CNRS 5250, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
Spinelli
Nicolas
N
Dumy
Pascal
P
Defrancq
Eric
E
D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010091:Oximes / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010091:Oximes