In vitro and in vivo evaluation of the prebiotics GroBiotic-A, inulin, mannanoligosaccharide, and galactooligosaccharide on the digestive microbiota and performance of hybrid striped bass (Morone chrysops x Morone saxatilis).
Two separate experiments were conducted with hybrid striped bass to evaluate four potential prebiotics: GroBiotic-A (partially autolyzed brewer's yeast, dairy ingredient components, and fermentation products), mannanoligosaccharide (MOS), galactooligosaccharide (GOS), and inulin. In the in vitro experiment, intestinal contents were incubated with the individual prebiotics (0.5% by weight) at 25 degrees C for 24 and 48 h. Analysis of volatile fatty acids in the supernatant showed that GroBiotic-A, MOS, and GOS tended to produce lower acetate levels but higher butyrate levels at 48 h compared to diet alone. However, denaturing gradient gel electrophoresis (DGGE) analysis failed to detect any differences in the composition of the microbial community among treatments. DNA sequencing of a common band for all inoculated samples revealed close similarity to the anaerobic Fusobacteria bacterium. An 8-week feeding trial also was conducted to evaluate the four prebiotics looking at growth performance; weight gain, feed efficiency ratio, protein efficiency ratio, whole-body ash, moisture, and lipid did not vary among fish fed the various diets. However, DGGE analysis revealed that all prebiotics produced a different type of microbial community in the intestinal tract of hybrid striped bass compared to fish fed the basal diet. Thus, GroBiotic-A, FOS, GOS, and MOS exhibited prebiotic effects in hybrid striped bass.
Burr
Gary
G
Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA. gary.burr@ars.usda.gov
Hume
Michael
M
Ricke
Steven
S
Nisbet
David
D
Gatlin
Delbert
D
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D001492:Bass / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D007444:Inulin / Q000494:pharmacology*; D023421:Models, Animal; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics
D005232:Fatty Acids, Volatile; D009844:Oligosaccharides; D056692:Prebiotics; D007444:Inulin