Polymeric nanoparticle-based activatable near-infrared nanosensor for protease determination in vivo.
We report here a new protease activatable strategy based on a polymer nanoparticle platform. This nanosensor delivers chemically labeled matrix metalloproteinase (MMP)-activatable fluorogenic peptides to the specific MMPs of interest in vivo. Intravenous administration of the nanosensor in an MMP-positive SCC-7 xenograft tumor and a colon cancer mouse model verified the enzyme specificity of the nanosensor in vivo. The design platform of the nanosensor is flexible and can be fine-tuned for a wide array of applications such as the detection of biomarkers, early diagnosis of disease, and monitoring therapeutic efficacy.
Lee
Seulki
S
Biomedical Research Center, Korea Institute of Science and Technology, Seongbuk-gu, Seoul 136-791, Korea.
Ryu
Ju Hee
JH
Park
Kyeongsoon
K
Lee
Aeju
A
Lee
Seung-Young
SY
Youn
In-Chan
IC
Ahn
Cheol-Hee
CH
Yoon
Soon Man
SM
Myung
Seung-Jae
SJ
Moon
Dae Hyuk
DH
Chen
Xiaoyuan
X
Choi
Kuiwon
K
Kwon
Ick Chan
IC
Kim
Kwangmeyung
K
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology*; D004337:Drug Carriers / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes; D020782:Matrix Metalloproteinases / Q000032:analysis*; D051379:Mice; D036103:Nanotechnology / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*; D014159:Transducers
D014408:Biomarkers, Tumor; D004337:Drug Carriers; D005456:Fluorescent Dyes; D011108:Polymers; D020782:Matrix Metalloproteinases