Identification of specific transmembrane residues and ligand-induced interface changes involved in homo-dimer formation of a yeast G protein-coupled receptor.
The Saccharomyces cerevisiae alpha-factor pheromone receptor, Ste2p, has been studied as a model for G protein-coupled receptor (GPCR) structure and function. Dimerization has been demonstrated for many GPCRs, although the role(s) of dimerization in receptor function is disputed. Transmembrane domains one (TM1) and four (TM4) of Ste2p were shown previously to play a role in dimerization. In this study, single cysteine substitutions were introduced into a Cys-less Ste2p, and disulfide-mediated dimerization was assessed. Six residues in TM1 (L64 to M69) that had not been previously investigated and 19 residues in TM7 (T278 to A296) of which 15 were not previously investigated were mutated to create 25 single Cys-containing Ste2p molecules. Ste2p mutants V68C in TM1 and nine mutants in TM7 (cysteine substituted into residues 278, 285, 289, and 291 to 296) showed increased dimerization upon addition of an oxidizing agent in comparison to the background dimers formed by the Cys-less receptor. The formation of dimers was decreased for TM7 mutant receptors in the presence of alpha-factor indicating that ligand binding resulted in a conformational change that influenced dimerization. The effect of ligand on dimer formation suggests that dimers are formed in the resting state and the activated state of the receptor by different TM interactions.
Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996, USA.
Heejung
H
Kim
Byung-Kwon
BK
Lee
Fred
F
Naider
Jeffrey M
JM
Becker
D019943:Amino Acid Substitution / Q000235:genetics; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D003553:Cystine / Q000378:metabolism; D005033:Ethylmaleimide / Q000378:metabolism / Q000494:pharmacology; D015951:Factor Xa / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D007700:Kinetics; D008024:Ligands; D000072235:Mating Factor; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000378:metabolism / Q000494:pharmacology; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D010618:Phenanthrolines / Q000378:metabolism / Q000494:pharmacology; D055503:Protein Multimerization / Q000187:drug effects / Q000235:genetics; D051964:Receptors, Mating Factor / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D000596:Amino Acids; D008024:Ligands; D009942:Organometallic Compounds; D016877:Oxidants; D010455:Peptides; D010618:Phenanthrolines; D051964:Receptors, Mating Factor; D011994:Recombinant Proteins; C498031:STE2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C464760:copper-1,10-phenanthroline; D003553:Cystine; D000072235:Mating Factor; D015951:Factor Xa; D003545:Cysteine; D005033:Ethylmaleimide