Beta-glucosidase coating on polymer nanofibers for improved  cellulosic ethanol production.
Beta-glucosidase (betaG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, betaG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The betaG coating (EC-betaG) was fabricated by crosslinking additional betaG molecules onto covalently attached betaG molecules (CA-betaG) via glutaraldehyde treatment. The initial activity of EC-betaG was 36 times higher than that of CA-betaG. After 20 days of incubation under shaking, CA-betaG and EC-betaG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of betaG coating. It is anticipated that the recycled uses of highly active and stable betaG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.
Lee
Sang-Mok
SM
Department of Biological Engineering, Inha University, Inchon 402-751, Korea.
Jin
Li Hua
LH
Kim
Jae Hyun
JH
Han
Sung Ok
SO
Na
Hyon Bin
HB
Hyeon
Taeghwan
T
Koo
Yoon-Mo
YM
Kim
Jungbae
J
Lee
Jung-Heon
JH
D002475:Cellobiose / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D000431:Ethanol; D005947:Glucose / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
D004800:Enzymes, Immobilized; D002475:Cellobiose; D000431:Ethanol; C427304:Novozym 188; D001617:beta-Glucosidase; D005947:Glucose