GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column.
Heptafluorobutyl chloroformate (HFBCF), a recently introduced derivatization reagent, was examined in enantioseparation of amino acids (AAs) by GC. Twenty proteinogenic AAs, plus ornithine, cystine and 4-fluorophenylalanine (internal standard) were treated with the reagent and separation properties of the derivatives were assessed on a Chirasil-Val capillary column. Nineteen AA enantiomers were efficiently separated in 43 min except proline, arginine and cystine. The HFBCF derivatives of the studied DL-AAs show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediate one-step reaction-extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of HFBCF to chiral AA analysis.
Zahradnícková
Helena
H
Department of Analytical Biochemistry, Biology Centre, Academy of Sciences of the Czech Republic, Ceské Budejovice, Czech Republic. helenaz@bclab.eu
Husek
Petr
P
Simek
Petr
P
D002849:Chromatography, Gas / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry*; D005561:Formates / Q000737:chemistry*; D057230:Limit of Detection; D015203:Reproducibility of Results
D005466:Fluorocarbons; D005561:Formates; C033094:perfluorobutyric acid; C083354:chlorocarbonic acid