1-Aminocyclopropane-1-carboxylic acid (ACC) concentration and ACC synthase expression in soybean roots, root tips, and soybean cyst nematode (Heterodera glycines)-infected roots.
Colonization of plant roots by root knot and cyst nematodes requires a functional ethylene response pathway. However, ethylene plays many roles in root development and whether its role in nematode colonization is direct or indirect, for example lateral root initiation or root hair growth, is not known. The temporal requirement for ethylene and localized synthesis of ethylene during the life span of soybean cyst nematode (SCN) on soybean roots was further investigated. Although a significant increase in ethylene evolution was not detected from SCN-colonized roots, the concentration of the immediate precursor to ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC), was higher in SCN-colonized root pieces and root tips than in other parts of the root. Moreover, expression analysis of 17 ACC synthase (ACS) genes indicated that a select set of ACS genes is expressed in SCN-colonized root pieces that is clearly different from the set of genes expressed in non-colonized roots or root tips. Semi-quantitative real-time PCR indicated that ACS transcript accumulation correlates with the high concentration of ACC in root tips. In addition, an ACS-like sequence was found in the public SCN nucleotide database. Acquisition of a full-length sequence for this mRNA (accession GQ389647) and alignment with transcripts for other well-characterized ACS proteins indicated that the nematode sequence is missing a key element required for ACS activity and therefore probably is not a functional ACS. Moreover, no significant amount of ACC was found in any growth stage of SCN that was tested.
Tucker
Mark L
ML
Soybean Genomics and Improvement Lab, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Ave., Beltsville, MD, USA. mark.tucker@ars.usda.gov
Xue
Ping
P
Yang
Ronghui
R
D000598:Amino Acids, Cyclic / Q000378:metabolism*; D000818:Animals; D005030:Ethylenes / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008190:Lyases / Q000235:genetics* / Q000378:metabolism; D018519:Meristem / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D009348:Nematoda / Q000254:growth & development / Q000502:physiology*; D010935:Plant Diseases / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*; D013025:Glycine max / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology
D000598:Amino Acids, Cyclic; D005030:Ethylenes; D010940:Plant Proteins; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase