[Influence of baicalin on TNF-alpha and soluble intercellular adhesion molecule-1 in rats infected with Pneumocystis carinii].
OBJECTIVE
To explore the effect of baicalin on tumour necrosis factor-alpha (TNF-alpha) and soluble intercellular adhesion molecule-1 (sICAM-1) of immunosuppressed rats infected with Pneumocystis carinii.


METHODS
Forty-nine SD rats were randomly divided into 7 groups: normal control (A), immunosuppressed control (B), SMZ/TMP control (C), baicalin prevention (D), low dose (E), moderate dose (F) and high dose (G). Rats of group D received six injections at three-day intervals with 3.5 mg dexamethasone sodium phosphate for 3 weeks, while groups B, C, E, F and G received same immunosuppressor but for 6 weeks. Rats in group D were given 100 mg/kg baicalin daily for 5 weeks, and groups C, E, F and G were given 250 mg/kg SMZ+50 mg/kg TMP, low dose [100mg/(kg x d)], moderate dose [200 mg/(kg x d)], and high dose [400 mg/(kg x d)] baicalin daily for 2 weeks, respectively. At the end of 8th week after immunosuppression, the contents of TNF-alpha and sICAM-1 in peripheral blood were detected by radioimmunoassay (RIA) and ELISA, respectively. The pathological change of lung was observed by lung imprint smear with gomori methenamine silver (GMS) staining and lung section with hematoxylin-eosin (HE) staining.


RESULTS
The content of TNF-alpha in group D [(2.14 +/- 0.14) ng/ml], group E [(2.57 +/- 0.15) ng/ml], group F [(1.46 +/- 0.14) ng/ml], group G [(1.12 +/- 0.13) ng/ml] and group C [(1.59 +/- 0.14) ng/ml] were higher than that of group A [(0.70 +/- 0.21) ng/ml] (P < 0.05, P < 0.01), and lower than group B [(3.65 +/- 0.73) ng/ml] (P < 0.01). The content of sICAM-1 in group D [(618.68 +/- 52.42) pg/ml], group E [(814.29 +/- 61.11) pg/ml], group F [(498.08 +/- 32.56) pg/ml], group G [(377.06 +/- 56.56) pg/ml] and group C [(386.95 +/- 44.98) pg/ml] were lower than group B [(1 247.39 +/- 288.57) pg/ml] (P < 0.05). Compared with immunosuppressed control group, there were less alveolar interstitial lymphocytes, foamy intra-alveolar exudate and inflammation of lung tissue in rats of drug treatment groups.


CONCLUSIONS
Baicalin can decrease the contents of TNF-alpha and sICAM-1, and alleviate inflammation in lung tissues of rats infected with Pneumocystis carinii.
Department of Traditional Chinese Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China.
Ling
L
Zhou
Bi-Ying
BY
Zhou
D000818:Animals; D005260:Female; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008517:Phytotherapy; D045363:Pneumocystis carinii; D011020:Pneumonia, Pneumocystis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D005419:Flavonoids; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; C038044:baicalin