The fundamental properties of Homer 1 in association with cardiac ryanodine receptor in mouse heart.
BACKGROUND
Homer, known as a scaffolding protein that regulates postsynapse signaling in neurons, has been poorly explored in cardiac research. We show the fundamental properties of Homer 1 in mouse heart in association with cardiac ryanodine receptor (RyR), a binding protein of Homer 1.


RESULTS
Immunohistochemistry of adult mouse heart with Homer 1 antibody showed striated staining on Z-bands both in atria and ventricles. The interactions between Homer 1 and RyR were confirmed by co-immunoprecipitation assays. Immunostaining of adult isolated cardiomyocytes showed partial co-localization of both proteins. In neonatal primary cultures, targeting of Homer 1 preceded that of RyR in their Z-band arrangement.


CONCLUSIONS
Homer 1 binds to RyR in adult mouse heart and precedes RyR in Z-band arrangement in the early postnatal period.
Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan. skawtsrg@tmd.ac.jp
Satoru
S
Kawaguchi
Satoshi
S
Shoji
Makoto
M
Sunamori
Teiichi
T
Furuichi
Seiko
S
Kawano
D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D000072233:Homer Scaffolding Proteins; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D012518:Sarcomeres / Q000378:metabolism
D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D019837:Ryanodine Receptor Calcium Release Channel