[Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes].
OBJECTIVE
To study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes.


METHODS
3T3-L1 preadipocytes transfected with either an empty expression vector (pcDNA3.1; control group) or an NYGGF4 expression vector (NYGGF4-pcDNA3.1) were cultured in vitro and differentiated into the matured adipocytes with the standard insulin plus dexamethasone plus 3-isobutyl-methylxanthine (MDI) induction cocktail. 2-deoxy-D-[3H] glucose uptake was determined by liquid scintillation counting. Western blot was performed to detect the protein content and translocation of glucose transporter 4 (GLUT4). The supernatant concentrations of TNF-alpha, IL-6, adiponectin and resistin were measured using ELISA.


RESULTS
NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake. NYGGF4 over-expression impaired insulin-stimulated GLUT4 translocation without affecting the total protein content of GLUT4. The concentrations of TNF-alpha, IL-6, adiponectin and resistin in the culture medium of 3T3-L1 transfected with NYGGF4 were not significantly different from those in the control group.


CONCLUSIONS
NYGGF4 over-expression impairs the insulin sensitivity of 3T3-L1 adipocytes through decreasing GLUT4 translocation and had no effects on the secretory function of adipocytes.
Nanjing Maternal and Child Health Care Hospital, Nanjing Medical University, Nanjing 210029, China.
Chun-Mei
CM
Zhang
Jie
J
Qiu
Xiao-Hui
XH
Chen
Bin
B
Wang
Min
M
Zhang
Xi-Rong
XR
Guo
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000032:analysis / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D052243:Resistin / Q000032:analysis; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D052242:Adiponectin; D002352:Carrier Proteins; D051275:Glucose Transporter Type 4; D007328:Insulin; D015850:Interleukin-6; C514653:PID1 protein, human; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D005947:Glucose