A new preservation solution (SCOT 15) Improves the islet isolation process from pancreata of non-heart-beating donors: a Murine model.
BACKGROUND
Due to the organ shortage, there is increased use of organs harvested from non-heart-beating donors (NHBD). These organs have been subjected to a period of warm ischemia that is most deleterious to functional recovery. We have designed a new preservation solution, "Solution de Conservation des Organes et des Tissus" (SCOT 15; Macopharma, Tourcoing, France) which contains an extracellular ionic composition including PEG 20 kD (15 g/L) as a colloid.


METHODS
Our objective was to compare SCOT 15 with University of Wisconsin (UW) solution or islet culture medium CMRL 1066 + 1% of Bovine Serum Albumin (BSA), as the working and preservation solution for islet isolation from pancreata subjected to warm ischemia using a murine model.


RESULTS
Warm ischemia decreased the islet yield and cellular viability regardless of the preservation solution. Either when the pancreas was or was not subjected to warm ischemia, the best islet yield was obtained with SCOT 15 (P < .05 vs UW or CMRL 1066). The same results were observed for islet viability as assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test; namely, better viability with SCOT 15 as compared with UW or CMRL 1066 (P < .01).


CONCLUSIONS
In a murine model SCOT 15 was a better preservation solution for islet isolation than UW solution or culture medium (CMRL 1066).
Giraud
S
S
Institut National de la Santé et de la Recherche Médicale U927, Paris, France.
Hauet
T
T
Eugene
M
M
Mauco
G
G
Barrou
B
B
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002102:Cadaver; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D005978:Glutathione; D006339:Heart Rate; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010101:Oxygen Consumption; D010179:Pancreas / Q000473:pathology; D011887:Raffinose; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms
D003470:Culture Media; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D012710:Serum Albumin, Bovine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose