Single-nucleotide polymorphisms in genes predisposing to asthma in children of Chinese Han nationality.
BACKGROUND
Research increasingly suggests that asthma is a familial and hereditary disorder in the pathogenesis of which genetic and environmental factors play an important role.


OBJECTIVE
To investigate the single and combined associations between 8 single-nucleotide polymorphism (SNP) loci in 5 genes and the development of asthma in children of Chinese Han nationality.


METHODS
The study population comprised 192 children with asthma and an equal number of healthy controls. Asthma was diagnosed in accordance with American Thoracic Society criteria. Polymerase chain reaction-restriction fragment length polymorphism was used to detect the genotypes of the SNP loci.


RESULTS
No statistically significant differences (P>.05) were found between the experimental and control group in genotype distribution among 6 loci (IL-13 C- 1112T, IL-13 C1923T, IL-4 C-590T, IL-4RA 175V, FcepsilonR1beta E237G, and beta2-ADR Q27E). However, significant diversity was observed among FcepsilonR1beta C-109T (P=.002) and beta2-ADR R16G (P=.000). Furthermore, the frequency of FcepsilonR1beta C-109T T/T and beta2-ADR R16G A/A in the asthma group was significantly higher than in the control group (odds ratio [OR]=1.96, P=.001; OR=2.58, P=.000, respectively). Carriers of both FcepsilonR1beta C-109T T/T and beta2-ADR R16G A/A had a more significant risk of developing asthma than those with only a single polymorphism.


CONCLUSIONS
The 6 loci (IL-13 C-1112T, IL-13 C1923T, IL-4 C-590T, IL-4RA 175V, FcER1B E237G and 12-ADR Q27E) make little contribution to the development of asthma in children of Chinese Han nationality. FcepsilonR1beta C-109T and beta2-ADR R16G are significantly associated with childhood asthma. FcepsilonR1beta C-109T T/T and beta2-ADR R16G A/A have a significant and combined effect on the development of asthma.
Department of Pediatrics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, China.
H
H
Li
D
D
Xiaoyan
L
L
Quanhua
L
L
Jie
B
B
Yixiao
D000293:Adolescent; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D055815:Young Adult
D017455:Receptors, IgE; D004247:DNA