Visual DNA as a diagnostic tool.
We report on the incorporation of the Visual DNA concept in a genotyping assay as a simple and straightforward detection tool. The principle of trapping streptavidin-coated superparamagnetic beads of micrometer size for visualization of genetic variances is used for PrASE-based detection of a panel of mutations in the severe and common genetic disorder of cystic fibrosis. The method allows a final investigation of genotypes by the naked eye and the output is easily documented using a regular hand-held device with an integrated digital camera. A number of samples were run through the assay, showing rapid and accurate detection using superparamagnetic beads and an off-the-shelf neodymium magnet. The assay emphasizes the power of Visual DNA and demonstrates the potential value of the method in future point-of-care tests.
Division of Gene Technology, AlbaNova University Center, Stockholm, Sweden.
Erik
E
Pettersson
Patrik L
PL
Ståhl
Hovsep
H
Mahdessian
Max
M
Käller
Joakim
J
Lundeberg
Afshin
A
Ahmadian
D000483:Alleles; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004247:DNA / Q000032:analysis*; D008280:Magnetics; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010447:Peptide Hydrolases; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA; D010447:Peptide Hydrolases