[Antibiotic-associated diarrhea due to Clostridium perfringens].
A 40-year-old man undergoing allo-hematopoietic stem cell transplantation for chronic myelogenous leukemia and developing diarrhea was administered prophylactic antibiotics including levofloxacin, fluconazole, cotrimoxazole, and vancomycin. Stool specimens were positive for toxin A in enzyme immunoassay but negative for toxin B in cell culture assay with a neutralization test, indicating that toxin A detection was false-positive. Stool culture yielded enterotoxin producing Clostridium perfringens, not Clostridium difficile. Polymerase chain reaction (PCR) detected the gene encoding C. perfringens enterotoxin in DNA extracted from stool specimens, but not the toxin B gene. Laboratory tests for enterotoxic C. perfingens may therefore be necessary for diagnosing antibiotic-associated diarrhea when culture for C. difficile is negative.
Division of Clinical Laboratory, Chiba Cancer Center Hospital.
Hideyuki
H
Satomura
Ikuko
I
Odaka
Chikara
C
Sakai
Haru
H
Kato
D000328:Adult; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D003015:Clostridium Infections / Q000209:etiology*; D003016:Clostridium perfringens; D003967:Diarrhea / Q000209:etiology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D008297:Male
D000900:Anti-Bacterial Agents