Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco.
OBJECTIVE
This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods.


METHODS
Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes.


RESULTS
Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb.


CONCLUSIONS
The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.
Ammari
Sanae
S
Service de recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger, Maroc.
Laglaoui
Amin
A
En-nanei
Latifa
L
Bertrand
Sophie
S
Wildemauwe
Christa
C
Barrijal
Said
S
Abid
Mohammed
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001434:Bacteriophage Typing; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009018:Morocco; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012480:Salmonella Infections / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins