Synthesis, conjugation, and immunological evaluation of the serogroup 6 pneumococcal oligosaccharides.
The first synthesis of the newly discovered oligosaccharide of pneumococcal serotype 6C and its spacer-containing analogue is reported. Conjugation of the spacer-containing oligosaccharides of pneumococcal saccharides 6A, 6B, 6C and derivatives thereof with bovine serum albumin (BSA) protein carrier was carried out by using squaric-acid approach to obtain the oligosaccharide-protein conjugates in excellent yields. The conjugates have been tested with a rabbit antiserum pool (Pool B) used for pneumococcal serotyping. The results showed that synthetic carbohydrate conjugates express epitopes found in native capsular polysaccharides of serotypes 6A, 6B, and 6C.
Parameswar
Archana R
AR
Department of Chemistry and Biochemistry, University of Missouri, St. Louis, 63121, USA.
Park
In Ho
IH
Saksena
Rina
R
Kovác
Pavol
P
Nahm
Moon H
MH
Demchenko
Alexei V
AV
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000378:metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000145:classification / Q000276:immunology*
D000907:Antibodies, Bacterial; D000939:Epitopes; D006001:Glycoconjugates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine