Decreased serum levels of carboxylesterase-2 in patients with ovarian cancer.
OBJECTIVE
Carboxylesterase-2 has been identified as the key enzyme in the metabolic activation ofirinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors. Previous studies have shown that carboxylesterase-2 is down-regulated in colorectal cancer following progression of the disease. However, very limited information is available on carboxylesterase-2 expression in ovarian cancer. The aim of the present study was to detect the serum level and the tissue expression of carboxylesterase-2 in human ovarian cancer patients at different stages of the disease.


METHODS
Carboxylesterase-2 levels in the serum of ovarian cancer patients were investigated by western blot and ELISA and in the tumor mass of ovarian cancer patients by western blot.


RESULTS
Both the serum carboxylesterase-2 level and the expression of carboxylesterase-2 in tumor tissues were significantly different among patients at different stages of the disease (n = 40). No positive correlation was found between the serum carboxylesterase-2 level and the cancer antigen 125 level (n = 40). Serum carboxylesterase-2 is more sensitive than cancer antigen 125 in detecting the early stage patient with ovarian cancer.


CONCLUSIONS
Our results indicate that serum carboxylesterase-2 level might be a potential marker in the diagnosis of the early stage ovarian cancer.
Department of Obstetrics and Gynecology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, PR China.
Liying
L
Cai
Xiaobo
X
Tang
Lei
L
Guo
Yuan
Y
An
Yuguang
Y
Wang
Jianhua
J
Zheng
D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D043182:Carboxylesterase / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000201:enzymology* / Q000473:pathology
D014408:Biomarkers, Tumor; C517204:CES2 protein, human; D043182:Carboxylesterase