Coupling tree-ring delta13C and delta15N to test the effect of fertilization on mature Douglas-fir (Pseudotsuga menziesii var. glauca) stands across the Interior northwest, USA.
Nitrogen (N) fertilization causes long-term increases in biomass production in many N-limited forests around the world, but the mechanistic basis underlying the increase is often unclear. One possibility, especially in summer-dry climates, is that N fertilization increases the efficiency with which a finite water supply is consumed to support photosynthesis. This increase is achieved by a reduction in the canopy-integrated concentration of internal CO(2) and thus discrimination against (13)C. We used stable isotopes of carbon (delta(13)C) in tree rings to experimentally test the physiological impact of N fertilization on mature Douglas-fir (Pseudotsuga menziesii Franco var. glauca) stands across the geographic extent of the Intermountain West, USA. The concentration and the stable isotopes of N (delta(15)N) in tree rings were also used to assess the presence and activity of fertilizer N. We hypothesized that N fertilization would (i) increase delta(15)N and N concentration of stemwood relative to non-fertilized stands and (ii) increase stemwood delta(13)C as photosynthetic gas exchange responded to the additional N. This experiment included two rates of urea addition, 178 kg ha(-1) (low) and 357 kg ha(-1) (high), which were applied twice over a 6-year interval bracketed by the 18 years of wood production measured in this study. Foliar N concentrations measured the year after each fertilization treatment suggest that the fertilizer N had been assimilated by the trees (P < 0.001). The N fertilization significantly enriched stemwood delta(15)N by 1.3 per thousand at the low fertilization rate and by 2.4 per thousand at the high rate (P < 0.001) despite variation in soil N between sites. However, we found no significant effect of the N fertilizer on delta(13)C of the annual rings (P = 0.76). These data lead us to suggest that alternative mechanisms underlie the growth response to fertilizer, i.e., increase in canopy area and shifts in biomass allocation.
Department of Soil Science, University of Wisconsin, Madison, WI 53706-1299, USA. njbalster@wisc.edu
Nick J
NJ
Balster
John D
JD
Marshall
Murray
M
Clayton
D002244:Carbon / Q000378:metabolism; D002247:Carbon Isotopes; D005308:Fertilizers; D009584:Nitrogen / Q000378:metabolism / Q000494:pharmacology; D009587:Nitrogen Isotopes; D015205:Northwestern United States; D028224:Pseudotsuga / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors
D002247:Carbon Isotopes; D005308:Fertilizers; D009587:Nitrogen Isotopes; D002244:Carbon; D009584:Nitrogen