Improved selective isolation of Bordetella pertussis by use of modified cyclodextrin solid medium.
We have developed a modified cyclodextrin solid (MCS) medium using the selective antibiotic cefdinir. MCS medium exhibited higher sensitivity (95.6%; any culture-positive sample as reference) and greater inhibition of nasopharyngeal flora than did Bordet-Gengou agar (65.2%, P = 0.009) or cyclodextrin solid medium (39.1%, P < 0.001).
Department of Infection Control Science, Faculty of Medicine, Juntendo University, 113-8421 Tokyo, USA.
Masayuki
M
Ohtsuka
Ken
K
Kikuchi
Kazuya
K
Shundo
Kenji
K
Okada
Masato
M
Higashide
Keisuke
K
Sunakawa
Keiichi
K
Hiramatsu
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001431:Bacteriological Techniques; D001886:Bordetella pertussis / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects; D000077525:Cefdinir; D002511:Cephalosporins / Q000494:pharmacology; D002648:Child; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D003505:Cyclodextrins; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D003470:Culture Media; D003505:Cyclodextrins; D000362:Agar; D000077525:Cefdinir