The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62).
Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication G- and R-banding patterns. Two sets of synchronized lymphocyte cultures were set up to obtain early (GBA+CBA-banding) and late (RBA-banding) BrdU incorporation. Slides were stained with acridine orange and observed under a fluorescence microscope. Reverse GBA+CBA- and RBA-banded karyotypes at the 450-band level were constructed. To verify G- and R-banding patterns in some acrocentric chromosomes, sequential GBA+CBA/Ag-NORs and RBA/Ag-NORs were also performed. The results of CBA-banding patterns obtained in 12 animals from 2 breeds showed a pronounced polymorphism of heterochromatin, especially in EAS1q-prox. Ideogrammatic representations of G- and R-banded karyotypes were constructed using only one common G- and R-banding nomenclature. In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level.
National Research Council (CNR), ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy.
G P
GP
Di Meo
A
A
Perucatti
V
V
Peretti
D
D
Incarnato
F
F
Ciotola
L
L
Liotta
T
T
Raudsepp
D
D
Di Berardino
B
B
Chowdhary
L
L
Iannuzzi
D000818:Animals; D001773:Blood Cells / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D002503:Centromere; D002871:Chromosome Banding / Q000662:veterinary*; D002874:Chromosome Mapping / Q000662:veterinary*; D004171:Diploidy; D018397:Equidae / Q000235:genetics*; D005260:Female; D007621:Karyotyping / Q000662:veterinary*; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics / Q000378:metabolism; D016622:Silver Staining