[Thermoregulation of catalytic activity of enzymes polymeric derivatives].
Trypsin was immobilized on polymeric carriers with low critical solution temperature (LCST). Homopolymer of N,N-diethylacrylamide (DEAA), random copolymers of DEAA and acrylamide (AA), and block copolymers polyDEAA-polyAA were used as the carriers. It was shown that at a temperature above LCST all carriers have a conformation change and trypsin's polymeric derivatives precipitate. The maximal activity after phase transition keeps trypsin, immobilized on polyDEAA block in polyDEAA-polyAA block-copolymer.
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D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006358:Hot Temperature; D014357:Trypsin / Q000737:chemistry*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D014357:Trypsin; D000099166:Polyacrylamides