Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction.
Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.
Intapan
Pewpan M
PM
Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Thailand. pewpan@kku.ac.th
Thanchomnang
Tongjit
T
Lulitanond
Viraphong
V
Pongsaskulchoti
Phunthira
P
Maleewong
Wanchai
W
D000818:Animals; D005243:Feces / Q000469:parasitology*; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008297:Male; D009891:Opisthorchis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity