Mercury(II) recognition and fluorescence imaging in vitro through a 3D-complexation structure.
8,8'-(1,4,10,13-Tetrathia-7,16-diazacyclooctadecane-7,16-diyl)-bis(methylene)diquinolin-7-ol (TTBQ) was synthesized and proved to selectively recognize Hg(2+), forming a Hg(2+)/TTBQ complex with an association constant (K(a)) as large as approximately 1.3 x 10(4) +/- 520 M(-1) in aqueous solution. Single crystals of TTBQ and a TTBQ-Hg(2+) complex have also been successfully grown, in which the latter unambiguously revealed a cagelike configuration consisting of thiol-crown and dual 7-hydroxyquinoline moieties to firmly trap Hg(2+). This 3D-complexation structure accounts for approximately 25-fold luminescence enhancement and a detection limit of sub-microM in water for sensing Hg(2+). Great selectivity toward Hg(2+) has been exhibited over alkali- and alkaline-earth metal ions, first-row transition-metal ions, and other cations studied. This chemosensor is particularly suited for the detection of Hg(2+) in a pH range of 5.5 to 7.5. This, in combination with its fine biocompatibility, leads to the success toward in vitro mercury recognition based on fluorescence imaging.
Ho
Mei-Lin
ML
Department of Chemistry, Soochow University, Taipei, Taiwan. meilin_ho@scu.edu.tw
Chen
Kew-Yu
KY
Lee
Gene-Hsiang
GH
Chen
Yu-Chun
YC
Wang
Chih-Chieh
CC
Lee
Jyh-Fu
JF
Chung
Wen-Chun
WC
Chou
Pi-Tai
PT
D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D018360:Crystallography, X-Ray; D005453:Fluorescence; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000737:chemistry*; D015394:Molecular Structure; D011804:Quinolines / Q000737:chemistry*; D013057:Spectrum Analysis
C530509:8,8'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)-bis(methylene)diquinolin-7-ol; D006573:Heterocyclic Compounds, 1-Ring; D011804:Quinolines; D008628:Mercury