Use of ion mobility mass spectrometry and a collision cross-section algorithm to study an organometallic ruthenium anticancer complex and its adducts with a DNA oligonucleotide.
We report the development of an enhanced algorithm for the calculation of collision cross-sections in combination with Travelling-Wave ion mobility mass spectrometry technology and its optimisation and evaluation through the analysis of an organoruthenium anticancer complex [(eta6-biphenyl)Ru(II)(en)Cl]+. Excellent agreement was obtained between the experimentally determined and theoretically determined collision cross-sections of the complex and its major product ion formed via collision-induced dissociation. Collision cross-sections were also experimentally determined for adducts of this ruthenium complex with the single-stranded oligonucleotide hexamer d(CACGTG). Ion mobility tandem mass spectrometry measurements have allowed the binding sites for ruthenium on the oligonucleotide to be determined.
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. J.P.Williams@warwick.ac.uk
Jonathan P
JP
Williams
Julie Ann
JA
Lough
Iain
I
Campuzano
Keith
K
Richardson
Peter J
PJ
Sadler
D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium; D017975:Ruthenium Compounds / Q000737:chemistry*
D000970:Antineoplastic Agents; D018736:DNA Adducts; D009841:Oligonucleotides; D009942:Organometallic Compounds; D017975:Ruthenium Compounds; D012428:Ruthenium