HIV-1 subtype B Tat gene activities and disease progression in HIV-1 CRF01_AE infection.
HIV-1 tat gene function and immunogenicity of HIV-1 Tat protein from 3 low (PS01, PS40, PS58) and 3 high (PS19, PS65, LP22) viral load infected, untreated and asymptomatic individuals from Thailand were compared. Levels of Tat-dependent chloramphenicol acetyltransferase (CAT) induced in HL3T1 cells with tat1 gene from HIV-1 isolates of high viral load group was significantly higher than those from low viral load group. HIV-1 subtype determination using env (C2-V4) gene demonstrated that 2/3 (PS01 and PS40) and 1/3 (PS58) from low viral load group were CRF01_AE and subtype B, while all 3 HIV-1 isolates from high viral load group were CRF01_AE. However, all 3 HIV-1 tat nucleotide sequences from low viral load group, which contained env CRF01_AE sequence, belonged to subtype B whereas all those from high viral load group contained CRF01_AE sequence. HIV Tat recombinant proteins from these groups were tested for immunogenicity in mice. All recombinant Tat proteins (except from PS58) were immunogenic in a dose-dependent manner, but with significantly differences of the immunogenicity levels between high and low viral load groups. These results indicated that HIV-1 subtype B tat gene activities might be associated with reduced disease progression of HIV-1 CRF01_AE infected individuals.
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Chaisit
C
Niyasom
Navin
N
Horthongkham
Apichai
A
Sreephiang
Wannee
W
Kantakamalakul
Suda
S
Louisirirotchanakul
Thippawan
T
Chuenchitra
Ruengpung
R
Sutthent
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018450:Disease Progression; D000939:Epitopes / Q000235:genetics; D005260:Female; D015756:Genes, tat / Q000502:physiology*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D011994:Recombinant Proteins; D019562:Viral Load; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000502:physiology*
D000939:Epitopes; D011994:Recombinant Proteins; D054299:env Gene Products, Human Immunodeficiency Virus