Anti-invasive activity of anthocyanins isolated from Vitis coignetiae in human hepatocarcinoma cells.
We investigated anti-invasive effects of the anthocyanins from fruits of Vitis coignetiae Pulliat (known as meoru in Korea) on human hepatoma Hep3B cells. The anthocyanins inhibited cell invasion in a dose-dependent manner as measured by Matrigel (BD Biosciences, San Jose, CA, USA) invasion assays. They also inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9 and activation of nuclear factor kappaB (NF-kappaB) stimulated by tumor necrosis factor alpha. Taken together, the results of this study indicate that the anthocyanins isolated from fruits of V. coignetiae Pulliat have anti-invasive effects on human hepatoma Hep3B cells and inhibit MMP-2 and MMP-9 gene expression at least in part through the inhibition of NF-kappaB activation.
Shin
Dong Yeok
DY
Department of Biochemistry, Dongeui University College of Oriental Medicine, Jeju, Republic of Korea.
Lee
Won Sup
WS
Kim
Seok Hyun
SH
Kim
Min Jeong
MJ
Yun
Jeong Won
JW
Lu
Jing Nan
JN
Lee
Sung Joong
SJ
Tsoy
Irina
I
Kim
Hye Jung
HJ
Ryu
Chung Ho
CH
Kim
Gi Young
GY
Kang
Ho Sung
HS
Shin
Sung Chul
SC
Choi
Yung Hyun
YH
D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009361:Neoplasm Invasiveness; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; D010936:Plant Extracts