Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation.
Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb(-/-) embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo. Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb(-/-) ES cells have a prolonged G(2)/M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.
Center for Experimental Medicine and Systems Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Masaki
M
Shibayama
Satona
S
Ohno
Takashi
T
Osaka
Reiko
R
Sakamoto
Akinori
A
Tokunaga
Yuhki
Y
Nakatake
Mitsuharu
M
Sato
Nobuaki
N
Yoshida
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D015152:Blotting, Northern; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D038941:Polypyrimidine Tract-Binding Protein / Q000235:genetics* / Q000378:metabolism*
D038941:Polypyrimidine Tract-Binding Protein