[Cloning and sequence analysis of the egG1Y162 gene of Echinococcus granulosus].
A pair of primers (egG1Y162) were designed according to the nucleotide sequence of Echinococcus multilocularis emY162 antigen gene. Using genomic DNA and cDNA from protoscoleces and adult worms of E. granulosus as templates, PCR was performed with the primers to obtain fragments of egG1Y162 gene. PUCm-T/egG1Y162 recombinant plasmids and PUCm-T/egY162 cDNA recombinant plasmids were constructed and identified by PCR, digestion with restriction enzyme and sequencing. The egG1Y162 antigen gene was amplified in protoscoleces and adult worms of E. granulosus. The size of the egG1Y162 gene was 1 648 bp and cDNA was 459 bp, and GenBank accession numbers were AB458258 and AB458259, respectively.
Xinjiang Key Laboratory of Echinococcosis, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China.
Chun-Bao
CB
Cao
Xiu-Min
XM
Ma
Jian-Bing
JB
Ding
Hai-Ying
HY
Jia
Wulamu
W
Mamuty
Hai-Mei
HM
Ma
Hao
H
Wen
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D048209:Echinococcus granulosus / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth