Synaptic capture-mediated long-lasting long-term potentiation is strongly dependent on mRNA translation.
In the CA1 region of mice hippocampal slices, a strong tetanic stimulation of an input pathway triggers a long-lasting long-term potentiation (L-LTP), which requires protein synthesis for the development of its late phase. A weak tetanic stimulation of one pathway, which is incapable of triggering protein synthesis on its own, can nonetheless induce L-LTP if it is preceded by a strong stimulation of another pathway (synaptic capture-mediated L-LTP). We found that anisomycin (25 microM), a translational inhibitor, impaired the strong stimulation-induced L-LTP more severely when the drug was applied during the whole experiment than when delivered only around the induction period. Taking advantage of this phenomenon, we showed that the synaptic capture-mediated L-LTP was strongly dependent on mRNA translation.
Ris
Laurence
L
Laboratory of Neurosciences, University of Mons, Mons, Belgium.
Villers
Agnès
A
Godaux
Emile
E
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004334:Drug Administration Schedule; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D011930:Reaction Time / Q000187:drug effects / Q000235:genetics; D009435:Synaptic Transmission / Q000187:drug effects / Q000235:genetics*; D013997:Time Factors
D009419:Nerve Tissue Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D000841:Anisomycin