Wide-field high-resolution surface-plasmon interference microscopy.
We describe a wide-field interferometric surface-plasmon microscope capable of submicrometer resolution. The system is a speckle-illuminated Linnik interferometer, which behaves as a wide-field analog of a scanning heterodyne interferometer. The presented images demonstrate contrast reversals at different defocus while retaining submicrometer lateral resolution. The contrast mechanisms are discussed as well as the instrumental requirements of the technique.
Somekh
Michael G
MG
Institute of Biophysics Imaging and Optical Science, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom. mike.somekh@nottingham.ac.uk
Stabler
Graham
G
Liu
Shugang
S
Zhang
Jing
J
See
Chung W
CW
D000465:Algorithms; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D004867:Equipment Design; D007368:Interferometry / Q000379:methods; D008853:Microscopy / Q000379:methods; D008857:Microscopy, Interference / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D020349:Surface Plasmon Resonance / Q000379:methods*
D012709:Serum Albumin