Müeller-Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus neoformans and Cryptococcus gattii to posaconazole with Etest.
Mueller-Hinton modified agar (MH-GMB) was compared with RPMI + 2% glucose-agar to determine the MICs of 80 isolates of Cryptococcus neoformans and C. gattii to posaconazole with Etest. MH-GMB minimised trailing and agreement between both media was 94%. Agreement of M27-A2 microbroth reference method was 98% with RPMI and 94% with MB-GMB.
Infectious Disease and Mycology Research Unit. Institut Municipal d 'Investigació Mèdica, Parc de Recerca Biomèdica de Barcelona, Barcelona, Spain. evictoriaalvarado@hotmail.com
Eidi
E
Alvarado-Ramírez
Josep M
JM
Torres-Rodríguez
Francisca
F
Murciano
Maite
M
Sellart
D000935:Antifungal Agents / Q000494:pharmacology*; D056285:Cryptococcus gattii / Q000187:drug effects*; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D003470:Culture Media; D014230:Triazoles; C101425:posaconazole