Fine-tuning of POPC liposomal leakage by the use of beta-cyclodextrin and several hydrophobic guests.
The effect of entrapped beta-cyclodextrin (beta-CD) on the stability of multilamellar vesicles (MLVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), prepared by the dehydration-rehydration method, was studied by monitoring the release of 5(6)-carboxyfluorescein encapsulated into the liposomes. Different hydrophobic guests, such as Fullerene C(60), have been incorporated into the POPC bilayer in order to modify the membrane composition. The kinetic results as well as ESI-MS measurements evidenced that the destabilizing activity of beta-CD is due to the formation of beta-CD inclusion complexes and the consequent removal of selected bilayer constituents from the liposomal membrane. Hence, when beta-CD was added to the liposomes in the form of a strong, water-soluble 2:1 beta-CD/C(60) inclusion complex, such a destabilizing effect was not observed. However, the same beta-CD/C(60) inclusion complex does not form as a result of C(60) extraction from the bilayer. This may be attributed either to the overwhelming concentration of POPC with respect to C(60) and/or to the fact that C(60) is largely aggregated in the bilayer. Turbidimetric and fluorimetric determinations of lamellarity and entrapped volume of the studied MLVs provided further evidence of the alteration of the liposomal bilayer as a consequence of the addition of beta-CD and/or the presence of the studied guests.
Dipartimento di Scienze del Farmaco, Università G. d'Annunzio, Chieti, Italy.
Carla
C
Gasbarri
Susanna
S
Guernelli
Simona
S
Boncompagni
Guido
G
Angelini
Gabriella
G
Siani
Paolo
P
De Maria
Antonella
A
Fontana
D004355:Drug Stability; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010713:Phosphatidylcholines / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
D008081:Liposomes; D010713:Phosphatidylcholines; D047392:beta-Cyclodextrins; C031215:betadex; C028694:1-palmitoyl-2-oleoylphosphatidylcholine