Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.
Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRalpha+Sca-1+CD45-TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.
Morikawa
Satoru
S
Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.
Mabuchi
Yo
Y
Kubota
Yoshiaki
Y
Nagai
Yasuo
Y
Niibe
Kunimichi
K
Hiratsu
Emi
E
Suzuki
Sadafumi
S
Miyauchi-Hara
Chikako
C
Nagoshi
Narihito
N
Sunabori
Takehiko
T
Shimmura
Shigeto
S
Miyawaki
Atsushi
A
Nakagawa
Taneaki
T
Suda
Toshio
T
Okano
Hideyuki
H
Matsuzaki
Yumi
Y
D000273:Adipose Tissue / Q000166:cytology / Q000528:radiation effects; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D019070:Cell Lineage / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000166:cytology / Q000528:radiation effects; D006410:Hematopoiesis; D045164:Mesenchymal Stem Cell Transplantation; D008648:Mesoderm / Q000166:cytology / Q000528:radiation effects; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000528:radiation effects; D010641:Phenotype; D011836:Radiation Tolerance; D014916:Whole-Body Irradiation