SELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstructive pulmonary disease.
OBJECTIVE
The aim of this work was to establish protein profiles in serum and nasal epithelial cells of cystic fibrosis individuals in comparison with controls, asthma and chronic obstructive pulmonary disease patients for specific biomarker signatures identification.


METHODS
Protein extracts were analyzed by Surface Enhanced Laser Desorption/Ionization Time-Of-Flight Mass-Spectrometry (SELDI-TOF-MS).


RESULTS
The mass spectra revealed a set of peaks with differential expression in serum and nasal cells among the different groups studied, resulting into peak signatures representative/specific of each pathology. Logistic regressions were applied to those peaks; sensitivity, specificity, Youden's indexes and area under the curve (AUC) of the respective receiver operating characteristic (ROC) curves were compared.


CONCLUSIONS
Multivariate analysis demonstrated that combination of peaks has a better predictive value than the individual ones. These protein signatures may serve as diagnostic/prognostic markers for the studied diseases with common clinical features, or as follow-up assessment markers of therapeutic interventions.
Gomes-Alves
Patrícia
P
Laboratório de Proteómica, Departamento de Genética, INSA-IP, Av Padre Cruz, 1649-016 Lisboa, Portugal.
Imrie
Margaret
M
Gray
Robert D
RD
Nogueira
Paulo
P
Ciordia
Sergio
S
Pacheco
Paula
P
Azevedo
Pilar
P
Lopes
Carlos
C
de Almeida
António Bugalho
AB
Guardiano
Micaela
M
Porteous
David J
DJ
Albar
Juan P
JP
Boyd
A Christopher
AC
Penque
Deborah
D
D000328:Adult; D000368:Aged; D001249:Asthma / Q000097:blood* / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood*; D003550:Cystic Fibrosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D040901:Proteomics / Q000379:methods; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000175:diagnosis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
D015415:Biomarkers