[The impact of melafen on the expression of chloroplastic chaperone protein HSP70B and photosynthetic pigments in cells of Chlamydomonas reinhardtii].
The effects of growth regulator of the new generation-melamine salt of bis(oxymethyl)phosphine acid (melafen)--on culture growth, pigment and protein content, and the induction of protective chloroplastic chaperone HSP70B in Chlamydomonas reinhardtii CW15 cells were studied. Melafen exhibited 10-30% growth inhibition at 10(-9)-10(-2)% concentration. At 10(-9)-10(-4)% of melafen electrophoretic concentration, the pattern of cellular proteins was similar to the control. The alterations in protein content of algae cells were detected only at 10(-2)% concentration. The content of chlorophyll and carotenoids in melafen-treated cells was 17-40% lower than in the control. Melafen at 10(-9)-109-2)% concentration inhibited HSP70B induction by 39-43% compared to untreated cells. The potential mechanism of melafen effect might involve its influence on nuclear gene expression.
Ermokhina
O V
OV
Belkina
G G
GG
Oleskina
Iu P
IuP
Fattakhov
S G
SG
Iurina
N P
NP
D020418:Algal Proteins / Q000096:biosynthesis*; D000818:Animals; D002338:Carotenoids / Q000096:biosynthesis; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism*; D002734:Chlorophyll / Q000096:biosynthesis; D002736:Chloroplasts / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D010721:Phosphinic Acids / Q000494:pharmacology*; D010940:Plant Proteins; D015800:Protozoan Proteins / Q000096:biosynthesis*; D014227:Triazines / Q000494:pharmacology*
D020418:Algal Proteins; D018840:HSP70 Heat-Shock Proteins; C102156:HSP70B protein, Chlamydomonas; D010721:Phosphinic Acids; D010940:Plant Proteins; D015800:Protozoan Proteins; D014227:Triazines; C503626:melaphene; D002734:Chlorophyll; D002338:Carotenoids