Neuroprotective action of FK-506 (tacrolimus) after seizures induced with pilocarpine: quantitative and topographic elemental analysis of brain tissue.
In the present work, X-ray fluorescence microscopy with a synchrotron source for the exciting radiation was applied for topographic and quantitative elemental analysis of rat brain tissue in pilocarpine-induced epilepsy and neuroprotection with FK-506. The mass per unit area of the elements P, S, Cl, K, Ca, Fe, Cu, Zn, Se, Br, and Rb was determined in four fields of the hippocampal formation (sectors 1 and 3 of Ammon's horn-CA1, CA3; dentate gyrus; hilus of dentate gyrus) and the parietal cortex. The results obtained for epileptic rats treated with FK-506 (SNF) were compared with data obtained previously for epileptic rats (SNS) and a control group. Many statistically significant differences in elemental composition were observed between the SNF and SNS groups. Higher mass per unit area of P was noticed in CA1 and CA3 regions of the hippocampus of SNF rats in comparison with SNS rats. A similar relation was observed for K in all five brain areas analyzed. Also, Fe in CA3 and dentate gyrus, Cu in the parietal cortex, and Zn in CA3 and in the cortex were present at a higher level in the SNF group in comparison with the SNS group. The findings obtained in the present study suggest that the neuroprotective action of FK-506 in epileptic rat brain may involve not only the inhibition of calcineurin but also blockade of the K(+) channels.
Department of Applied Nuclear Methods, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Kraków, Poland. jchwiej@novell.ftj.agh.edu.pl
Joanna
J
Chwiej
Krzysztof
K
Janeczko
Marianna
M
Marciszko
Mateusz
M
Czyzycki
Karen
K
Rickers
Zuzanna
Z
Setkowicz
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced* / Q000517:prevention & control*; D013052:Spectrometry, X-Ray Emission; D017356:Synchrotrons; D016559:Tacrolimus / Q000494:pharmacology*
D018696:Neuroprotective Agents; D010862:Pilocarpine; D016559:Tacrolimus