Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana.
Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.
Deguchi
Tomonori
T
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakouji, Amagasaki, Hyogo, Japan.
Itoh
Mariko
M
Urawa
Hiroko
H
Matsumoto
Tomohiro
T
Nakayama
Sohei
S
Kawasaki
Takashi
T
Kitano
Takeshi
T
Oda
Shoji
S
Mitani
Hiroshi
H
Takahashi
Taku
T
Todo
Takeshi
T
Sato
Junichi
J
Okada
Kiyotaka
K
Hatta
Kohei
K
Yuba
Shunsuke
S
Kamei
Yasuhiro
Y
D000818:Animals; D030801:Animals, Genetically Modified; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018390:Gene Targeting / Q000379:methods; D017930:Genes, Reporter / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007259:Infrared Rays; D007834:Lasers; D008954:Models, Biological; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000528:radiation effects; D015533:Transcriptional Activation / Q000528:radiation effects*; D019076:Transgenes / Q000502:physiology / Q000528:radiation effects; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins