Production and characterization of a novel laccase with cold adaptation and high thermal stability from an isolated fungus.
A new white-rot fungus SYBC-L1, which could produce an extracellular laccase, was isolated from a decayed Elaeocarpus sylvestris. The strain was identified as Pycnoporus sp. SYBC-L1 according to the morphological characteristics and ribosomal ITS1-5.8S-ITS2 RNA genomic sequence analysis. The highest laccase activity of 24.1 U ml(-1), which was approximately 40-fold than that in basal medium, was achieved in optimal culture medium in submerged fermentation. The laccase produced by Pycnoporus sp. SYBC-L1 was not only a cold adaptation enzyme with a relative catalytic activity of 30.2% at 0 degrees C but also a high thermostable enzyme. The half-lives at 60, 70 and 80 degrees C were 85.5, 37.2, and 2.6 h, respectively. The laccase could effectively decolorize weak acid blue AS and diamond black PV up to 88% and 74.7%, respectively, within 2 h in the absence of any redox mediators. The results suggested Pycnoporus sp. SYBC-L1 was a potential candidate for laccase production and industrial application.
Wang
Zhixin
Z
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Cai
Yujie
Y
Liao
Xiangru
X
Zhang
Feng
F
Zhang
Dabing
D
Li
Zhiling
Z
D000222:Adaptation, Physiological; D000818:Animals; D002244:Carbon / Q000494:pharmacology; D003080:Cold Temperature; D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D045467:Elaeocarpaceae / Q000382:microbiology; D004795:Enzyme Stability; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007101:Immersion; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology; D055494:Pycnoporus / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification / Q000502:physiology*; D014935:Wool
D004396:Coloring Agents; D002244:Carbon; D003300:Copper; D042845:Laccase; D009584:Nitrogen