Developmental expression of Smoc1 and Smoc2 suggests potential roles in fetal gonad and reproductive tract differentiation.
SMOC1 and SMOC2 are matricellular proteins thought to influence growth factor signaling, migration, proliferation, and angiogenesis. We examined the expression and regulation of Smoc1 and Smoc2 in fetal gonad/mesonephros complexes to discover possible roles for these genes in gonad and mesonephros development. Smoc1 was upregulated at approximately E10.75 in a center-to-poles wave in pre-Sertoli and pre-granulosa cells and its expression was greatly reduced in Wt1, Sf1, and Fog2 mutants. After E13.5, Smoc1 was downregulated in an anterior-to-posterior wave in granulosa cells but persisted in Sertoli cells, suggesting a sexually dimorphic requirement in supporting cell lineage differentiation. Smoc2 was expressed in Leydig cells, mesonephroi, and Wnt4 mutant ovaries, but not wildtype ovaries. Using organ culture, we determined that Smoc2 expression was dependent on Hedgehog signaling in testes, mesonephroi, and kidneys. Overall, these results demonstrate that SMOC1 and SMOC2 may mediate intercellular signaling and cell type-specific differentiation during gonad and reproductive tract development.
Pazin
Dorothy E
DE
Genetics Program, Department of Medicine, and Graduate Program in Genetics and Genomics, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Albrecht
Kenneth H
KH
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D005260:Female; D053823:Hedgehog Proteins / Q000378:metabolism*; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D008650:Mesonephros / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015676:Osteonectin / Q000378:metabolism*; D010053:Ovary / Q000196:embryology* / Q000378:metabolism; D000072260:RNA Splicing Factors; D012708:Sertoli Cells / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013737:Testis / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D060528:Wnt4 Protein
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D053823:Hedgehog Proteins; D015676:Osteonectin; D000072260:RNA Splicing Factors; C524549:SMOC-1 protein, mouse; C513851:SMOC-2 protein, mouse; C491514:Sf1 protein, mouse; D014157:Transcription Factors; D025721:WT1 Proteins; D051153:Wnt Proteins; D060528:Wnt4 Protein; C555582:Wnt4 protein, mouse; C492170:Zfpm2 protein, mouse; D014212:Tretinoin