Enhanced amplification of GC-rich DNA with two organic reagents.
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
Zhang
Zhizhou
Z
HIT Bio-X Centre for Systems Biotechnology, Harbin Institute of Technology, Weihai, China. zhangzzbiox@gmail.com
Yang
Xia
X
Meng
Liangyu
L
Liu
Fang
F
Shen
Cenchao
C
Yang
Wenjuan
W
D019855:Ethylene Glycol; D020862:GC Rich Sequence; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019946:Propylene Glycol; D017422:Sequence Analysis, DNA / Q000379:methods*
D019946:Propylene Glycol; D019855:Ethylene Glycol