Copper redistribution in Atox1-deficient mouse fibroblast cells.
Quantitative synchrotron X-ray fluorescence (SXRF) imaging of adherent mouse fibroblast cells deficient in antioxidant-1 (Atox1), a metallochaperone protein responsible for delivering Cu to cuproenzymes in the trans-Golgi network, revealed striking differences in the subcellular Cu distribution compared with wild-type cells. Whereas the latter showed a pronounced perinuclear localization of Cu, the Atox1-deficient cells displayed a mostly unstructured and diffuse distribution throughout the entire cell body. Comparison of the SXRF elemental maps for Zn and Fe of the same samples showed no marked differences between the two cell lines. The data underscore the importance of Atox1, not only as a metallochaperone for delivering Cu to cuproenzymes, but also as a key player in maintaining the proper distribution and organization of Cu at the cellular level.
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332, USA.
Reagan
R
McRae
Barry
B
Lai
Christoph J
CJ
Fahrni
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D027682:Cation Transport Proteins / Q000172:deficiency* / Q000378:metabolism; D002460:Cell Line; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D000079922:Copper Transport Proteins; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018832:Molecular Chaperones / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission; D021601:trans-Golgi Network / Q000187:drug effects / Q000378:metabolism
C499247:Atox1 protein, mouse; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; D003470:Culture Media; D018832:Molecular Chaperones; D003300:Copper; C029892:cupric chloride