Monoglyceride and diglyceride production through lipase-catalyzed glycerolysis and molecular distillation.
Distilled glycerides are obtained through distillation of the system mono-diglycerides which is produced from the esterification reaction between a triglyceride with glycerol. In this work, monoglycerides (MG) and diglycerides (DG) are produced through lipase-catalyzed glycerolysis of soybean oil using Candida antarctica B in a solvent-free system. To separate the products of the reaction in order to obtain essentially MG and an oil of DG, it is necessary to use a suitable process in order to preserve the stability of the components and to keep the products free of inappropriate solvents. So, after 24 h of enzymatic reaction, the mixture of acylglycerols and fatty acids was distilled into a centrifugal molecular distiller, since it provides a free solvent and lower temperature environment to increase the desired product concentration. Starting from a material with 25.06% of triglycerides (TG), 46.63% of DG, 21.72% of MG, 5.38% of free fatty acids (FFA), and 1.21% of glycerol, the MG purity in the distillate stream was 80% at evaporator temperature (T (E)) equal to 250 degrees C and feed flow rate (Q) equal to 10.0 mL/min. At these conditions, the MG recovery was 35%. The material collected in the residue stream presented DG-enriched oil with TG unhydrolyzed, residual MG, and low acidity (29.83% of TG, 53.20% of DG, 15.64% of MG, and 1.33% of FFA), which is suitable to replace TG oil in the human diet.
Fregolente
Patricia Bogalhos Lucente
PB
Separation Process Development Laboratory, Chemical Engineering School, State University of Campinas, 13081970 Campinas, São Paulo, Brazil. lucente@feq.unicamp.br
Pinto
Gláucia Maria F
GM
Wolf-Maciel
Maria Regina
MR
Filho
Rubens Maciel
RM
D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D002498:Centrifugation / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005656:Fungal Proteins; D005990:Glycerol / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D013024:Soybean Oil / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D050178:Monoglycerides; D013024:Soybean Oil; D008049:Lipase; C431349:lipase B, Candida antarctica; D005990:Glycerol