Comparison of the performance of two mycobacteriophage D29-based protocols for fluoroquinolone susceptibility testing in Mycobacterium tuberculosis.
We tested a mycobacteriophage D29-based method for fluoroquinolone susceptibility assessment in clinical isolates of Mycobacteriumtuberculosis. The method was incapable of identifying susceptible strains as such, although a slightly different published protocol successfully identified resistant and susceptible strains. Thus, caution is necessary when choosing an "in-house" D29-based protocol for testing of drug resistance.
Cátedra de Microbiología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, 2000 Rosario, Argentina.
Emma J
EJ
Stella
Agustina I
AI
de la Iglesia
Héctor R
HR
Morbidoni
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009160:Mycobacteriophages / Q000187:drug effects / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones