A food safety control low mass-range proteomics platform for the detection of illicit treatments in veal calves by MALDI-TOF-MS serum profiling.
Performance enhancing agents (PEAs) are illegally used in cattle and other meat producing species to increase food conversion and lean meat production. Due to the very short breeding cycle, veal calves represent the meat producing bovine category mostly subjected to illicit treatments. These chemical agents are difficult to detect by conventional analytical approaches due to the employment of synergistic formulations at very low dosage and given the use of uncharacterized novel compounds. Such a scenario has fostered a strong interest in the discovery of functional molecular biomarkers for the detection of growth promoting agents in meat producing species. A multivariate MALDI-TOF-MS proteomics platform has been developed using bovine serum samples. Analytical performances have been thoroughly evaluated in order to enable reproducible profiles from 10 microL sera samples. We propose univariate and multivariate discrimination models capable to identify calves undergoing illicit treatments. In particular, we found a strong discrimination power associated with a polypeptide fragment from beta2-glycoprotein-I. We provide a fundamental proof of concept in the potential application of MALDI-TOF-MS proteomics profiling in the food safety control.
Department of Animal Pathology, Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Università di Torino, Grugliasco, Turin, Italy.
Lorenza
L
Della Donna
Maurizio
M
Ronci
Paolo
P
Sacchetta
Carmine
C
Di Ilio
Bartolomeo
B
Biolatti
Giorgio
G
Federici
Carlo
C
Nebbia
Andrea
A
Urbani
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle / Q000097:blood*; D005513:Food Inspection / Q000379:methods*; D008297:Male; D008460:Meat / Q000592:standards*; D040901:Proteomics / Q000379:methods*; D012372:ROC Curve; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods / Q000662:veterinary*
D015415:Biomarkers