The range of hemoglobin A(2) in hemoglobin E heterozygotes as determined by capillary electrophoresis.
Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A(2) (HbA(2)), thus permitting quantification of HbA(2) in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA(2) of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA(2) of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA(2) values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA(2) in patients with HbE when evaluated by CE.
Department of Pathology, St Joseph Mercy Hospital, Ann Arbor, MI 48106, USA.
Daniel David
DD
Mais
Ronald D
RD
Gulbranson
David F
DF
Keren
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D006443:Hemoglobin A2 / Q000032:analysis* / Q000235:genetics; D006446:Hemoglobin E / Q000032:analysis* / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D012016:Reference Values; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis / Q000235:genetics
D006443:Hemoglobin A2; D006446:Hemoglobin E