D-amino acid oxidase generates agonists of the aryl hydrocarbon receptor from D-tryptophan.
The aryl hydrocarbon receptor (AHR) is well-known for its role in mediating the toxic and adaptive responses to xenobiotic compounds. Recent studies also indicate that AHR ligands are endogenously produced and may be essential for normal development. Previously, we showed that the endogenous enzyme, aspartate aminotransferase (AST), generates the AHR proagonist, indole-3-pyruvic acid (I3P), by deamination of its substrate L-tryptophan. We hypothesized that other enzymatic pathways capable of producing I3P may generate AHR agonists in vivo. We now demonstrate that the enzyme d-amino acid oxidase (DAAO) catalyzes the production of AHR agonists through the enzymatic conversion of D-tryptophan to I3P. Moreover, we provide evidence that the nonenzymatic oxidation and condensation of I3P is a critical step in the generation of receptor agonists by DAAO and AST. Products of this process include two novel agonists, 1,3-di(1H-indol-3-yl)propan-2-one and 1-(1H-indol-3-yl)-3-(3H-indol-3-ylidene) propan-2-one [characterized in the accompanying paper, Chowdhury et al. ( 2009 ) Chem. Res. Toxicol. , DOI: 10.1021/tx9000418 ], both of which can potently activate the AHR at concentrations in the nanomolar range. These results show that endogenous AHR activity can be modulated by I3P production from amino acid precursors through multiple enzymatic pathways, including those catalyzed by DAAO and AST.
McArdle Laboratory, Department of Oncology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA.
Linh P
LP
Nguyen
Erin L
EL
Hsu
Goutam
G
Chowdhury
Miroslav
M
Dostalek
F Peter
FP
Guengerich
Christopher A
CA
Bradfield
D045744:Cell Line, Tumor; D003605:D-Amino-Acid Oxidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000302:isolation & purification; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists* / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism*
C547592:1,3-di(1H-indol-3-yl)propan-2-one; C547593:1-(1H-indol-3-yl)-3-(3H-indol-3-ylidene)propan-2-one; D007211:Indoles; D018336:Receptors, Aryl Hydrocarbon; C008122:indol-3-yl pyruvic acid; D014364:Tryptophan; D003605:D-Amino-Acid Oxidase