Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): gene, inductive expression pattern and subcellular localization.
Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper.
Pearl River Fishery Research Institute, Chinese Academic of Fishery Science, Guangzhou, 510380, China.
Yan
Y
Shi
Zhe
Z
Zhao
Jing-Kui
JK
Yin
Xin-Ping
XP
Zhu
Kun-Ci
KC
Chen
De-Bo
DB
Pan
Jian-Fang
JF
Gui
D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D016678:Genome; D050836:Interferon Regulatory Factor-2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D021381:Protein Transport; D015533:Transcriptional Activation / Q000187:drug effects
D050836:Interferon Regulatory Factor-2; D008070:Lipopolysaccharides; D011070:Poly I-C