Glutathione and malondialdehyde levels in late pulmonary complications of sulfur mustard intoxication.
It has been hypothesized that antioxidant and oxidant capacities may be related to the severity of obstructive lung impairment in patients with sulfur mustard (SM)-induced lung injuries. Our study was designed to measure the level of glutathione (GSH) and malondialdehyde (MDA) activities in patients intoxicated with SM and to evaluate the relationship between their activity and the severity of pulmonary dysfunction. A total of 250 patients with a history of exposure to a single high dose of SM gas and also 60 healthy nonsmoking individuals with no history of exposure to SM were selected. All patients underwent spirometry; based on its indices they were divided into two groups: mild (n = 140) and moderate-to-severe (n = 110) pulmonary dysfunction. Also, serum GSH and MDA concentration measurements were performed for all patients and controls. The mean GSH level in controls was 29.85 +/- 3.26 micromol/ml, which was significantly higher than in patients with mild and moderate-to-severe pulmonary dysfunction (19.02 +/- 2.36 and 17.89 +/- 2.16 micromol/ml, respectively). Also, the mean MDA level in controls was 0.69 +/- 0.09 micromol/ml, which was significantly lower than in patients with mild and moderate-to-severe pulmonary dysfunction (0.74 +/- 0.05 and 0.75 +/- 0.05 micromol/ml, respectively). There was a weak linear correlation between GSH level and some of the pulmonary function indices. On the other hand, there was no significant relationship between the MDA level and pulmonary indices. Our study confirmed important alterations in the oxidative-antioxidative system in patients suffering from SM-induced lung injuries, as shown by a decreased serum level of GSH and an increased level of MDA. Individuals with moderate-to-severe SM-induced lung injuries show a greater tendency for a decreased level of GSH and an increased level of MDA than those with mild injuries; however, there is only minimal association between pulmonary function parameters and the serum level of MDA and GSH. These findings encourage us to examine therapeutic measures to correct such imbalances in future studies.
Research Center of Chemical Injuries, Baqiyatallah Medical Sciences University, Mollasadra Street, P.O. Box 19945-546, Tehran, Iran. shohratimajid@yahoo.com
Majid
M
Shohrati
Mostafa
M
Ghanei
Navvab
N
Shamspour
Fatemeh
F
Babaei
Majid Norozi
MN
Abadi
Mahvash
M
Jafari
Ali Amini
AA
Harandi
Amini Harandi
AH
Ali
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002619:Chemical Warfare Agents / Q000506:poisoning*; D005978:Glutathione / Q000097:blood*; D006801:Humans; D019570:Inhalation Exposure; D007492:Iran; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D055370:Lung Injury / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009151:Mustard Gas / Q000506:poisoning*; D018384:Oxidative Stress / Q000187:drug effects; D012720:Severity of Illness Index; D013147:Spirometry
D015415:Biomarkers; D002619:Chemical Warfare Agents; D008315:Malondialdehyde; D005978:Glutathione; D009151:Mustard Gas