An albumin-conjugated peptide exhibits potent anti-HIV activity and long in vivo half-life.
The clinical application of conventional peptide drugs often is limited by their short in vivo half-life and potential immunogenicity. Frequent injection presents challenges to the treatment of chronic diseases, such as HIV infection. We chemically modified a peptide HIV fusion inhibitor with 3-maleimidopropionic acid (MPA), which allows rapid and irreversible conjugation with serum albumin at a 1:1 molar ratio. FB006M, with an MPA modification at the 13th amino acid, rapidly formed conjugate with albumin upon intravenous injection, and it exhibited a remarkably extended in vivo half-life. The albumin conjugate of FB006M displayed potent inhibitory activity against a number of laboratory and clinical isolates of HIV-1 in vitro and in vivo. No immunogenicity or antibody formation was detected after repeated dosing. The clinical application of FB006M may decrease the cost of treatment and improve treatment compliance and patient quality of life.
Xie
Dong
D
Chongqing Frontier Biotechnologies Co., 3rd Floor, Bldg. I, 70 Keyuan 4th St., Bio-Garden, High-Tech Zone, Chongqing 400041, China. dxie@frontierbiotech.com
Yao
Cheng
C
Wang
Li
L
Min
Wenjie
W
Xu
Jiahong
J
Xiao
Jiahai
J
Huang
Mingxian
M
Chen
Bo
B
Liu
Bin
B
Li
Xiaolin
X
Jiang
He
H
D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000493:pharmacokinetics* / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015700:HIV Envelope Protein gp41 / Q000187:drug effects / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects*; D006207:Half-Life; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects / Q000821:virology; D010455:Peptides / Q000493:pharmacokinetics* / Q000494:pharmacology* / Q000633:toxicity; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000737:chemistry*
D019380:Anti-HIV Agents; D015700:HIV Envelope Protein gp41; D010455:Peptides; D012709:Serum Albumin