Bioassay-guided separation of the proinflammatory constituents from the roots of Euphorbia kansui.
In view of the toxic inflammatory reaction induced by Euphorbia kansui roots, a traditional Chinese medicine used for the treatment of edema, ascites, and asthma, the 95% ethanol extract was found to have a significant stimulating effect on inflammatory cells. Bioassay-guided separation of the 95% ethanol extract from the roots of E. kansui led to the isolation of five diterpenoids whose structures were identified by (1)H, (13)C NMR spectroscopy and HR-ESI-MS as kansuinine B (1), kansuinine A (2), kansuiphorin C (3), 3-O-benzoyl-20-deoxyingenol (4), and 3-O-(2'E,4'Z-decadienoyl)-20-O-acetylingenol (5). The proinflammatory effect of compounds 1-5 was evaluated in vitro in models of inflammation using exoteric mice splenic lymphocytes (SPL) and rat peritoneal macrophages (PMphi). Compounds 1, 2, and 5 markedly promoted SPL proliferation and NO production by PMphi at concentrations from 0.78 to 12.50 microg/mL. Hence the three compounds are believed to be important proinflammatory components of the roots of E. kansui.
Shu
Xiaoyun
X
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, China.
Yu
Li
L
Tang
Yuping
Y
Zhang
Li
L
Ding
Anwei
A
Luo
Dan
D
Duan
Jin-ao
JA
Shen
Xiangchun
X
D000818:Animals; D001681:Biological Assay / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D004224:Diterpenes; D010936:Plant Extracts; D009569:Nitric Oxide