Fast ion chromatography using short anion exchange columns.
A fast ion chromatographic system is described which uses shorter column lengths and compares various eluent profiles in order to maximise the performance without sacrificing the chromatographic resolution. Both isocratic and gradient elution profiles were considered to find the most efficient mode of separation. The separation and determination of seven target anions (chloride, chlorate, nitrate, chromate, sulfate, thiocyanate and perchlorate) was achieved using a short (4mm ID, 50mm long) column packed with Dionex AS20 high-capacity anion exchange material. A hydroxide eluent was used at an initial concentration of 25 mM (at a flow-rate of 1.0 mL/min) and two performance maxima were found. The maximum efficiency occurred at a normalised gradient ramp rate of 5mM/t(0), resulting in a peak capacity of 16, while the fastest separation (<3 min) occurred at a normalised ramp rate of 30 mM/t(0). The retention time, peak width and resolution using the different eluent profiles on varying column lengths is also compared. Further investigations in this study determined that the highest peak capacity separation under gradient conditions could be approximated using an isocratic separation. The advantage of using this novel approach to approximate the maximum efficiency separation removes the need for column re-equilibration that is required for gradient elution resulting in faster analyses and enhanced sample throughput, with benefits in particular for multidimensional chromatography.
Tyrrell
Eadaoin
E
Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia.
Shellie
Robert A
RA
Hilder
Emily F
EF
Pohl
Christopher A
CA
Haddad
Paul R
PR
D000838:Anions / Q000737:chemistry*; D002712:Chlorides / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods* / Q000592:standards; D010472:Perchlorates / Q000302:isolation & purification; D013997:Time Factors
D000838:Anions; D002712:Chlorides; D010472:Perchlorates; C494474:perchlorate