[Mechanism of C-reactive protein on proliferation of multiple myeloma U266 cells].
This study was purposed to investigate the mechanism of C-reactive protein (CRP) on proliferation of U266 cells. The human multiple myeloma cell line U266 was incubated with human CRP (0, 5, 10, 20 mg/L) for 24 hours, then the proliferation level of U266 cells was detected by using blood analyser. The mRNA expressions of survivin and HSP90alpha were examined by RT-PCR. The results showed that the proliferation ratio was increased, as compared with the control group (p<0.05); furthermore, the mRNA levels of survivin and HSP90alpha were up-regulated in proportion to the increased CRP concentrations. There was significant correlation between expression of survivin and HSP90alpha (r=0.737, p<0.0001) in incubated cells. It is concluded that CRP can stimulate the proliferation of MM cells directly by up-regulating the expression of survivin and HSP90alpha in MM cells. CRP can be regarded as a potential target for MM treatment.
Yang
Yi-Rong
YR
Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
Huang
Ling-Juan
LJ
Ma
Yan-Ping
YP
Lu
Yu-Jin
YJ
Yang
Lin-Hua
LH
Zhou
Yong-An
YA
D017209:Apoptosis; D002097:C-Reactive Protein / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D000077022:Survivin
C107489:BIRC5 protein, human; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; D002097:C-Reactive Protein