Study of peptide-protein interaction by high-performance liquid chromatography. Effect of hydrophobic and steric parameters.
The interaction of 16 peptides was studied on zein-coated silica column by high-performance liquid chromatography. The separation factor, theoretical plate number and the asymmetry factors of the peaks were calculated. Stepwise regression analysis was applied to study the peptide-protein interactions and to find the molecular substructures involved in the interactions and to define their character. Zein-coated silica column was suitable for the determination of peptide-protein interaction and the investigated peptide-protein interactions were found to be mixed (hydrophobic and steric) character.
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest, Hungary.
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Szikszay
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Forgács