Optimization and validation of a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of nicotine, cotinine, trans-3'-hydroxycotinine and norcotinine in human oral fluid.
An analytical procedure was developed and validated for the simultaneous identification and quantification of nicotine, cotinine, trans-3'-hydroxycotinine, and norcotinine in 0.5 mL of human oral fluid collected with the Quantisal oral fluid collection device. Solid phase extraction and liquid chromatography-tandem mass spectrometry with multiple reaction monitoring were utilized. Endogenous and exogenous interferences were extensively evaluated. Limits of quantification were empirically identified by decreasing analyte concentrations. Linearity was from 1 to 2,000 ng/mL for nicotine and norcotinine, 0.5 to 2,000 ng/mL for trans-3'-hydroxycotinine, and 0.2 to 2,000 ng/mL for cotinine. Correlation coefficients for calibration curves were >0.99 and analytes quantified within +/-13% of target at all calibrator concentrations. Suitable analytical recovery (>91%) was achieved with extraction efficiencies >56% and matrix effects <29%. This assay will be applied to the quantification of nicotine and metabolites in oral fluid in a clinical study determining the most appropriate nicotine biomarker concentrations differentiating active, passive, and environmental nicotine exposure.
Shakleya
Diaa M
DM
Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Biomedical Research Center, 251 Bayview Boulevard Suite 200, Room 05A-721, Baltimore, MD 21224, USA. shakleyad@intra.nida.nih.gov
Huestis
Marilyn A
MA
D002853:Chromatography, Liquid / Q000379:methods; D003367:Cotinine / Q000031:analogs & derivatives* / Q000737:chemistry*; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009538:Nicotine / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C066333:norcotinine; C001381:hydroxycotinine; D009538:Nicotine; D003367:Cotinine