Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.).
Warty fruit is one of the highly valuable external quality traits related to the market values of cucumber. Genetic analysis has shown that a single dominant gene, Tu (Tuberculate fruit), determines the warty fruit trait in the cucumber plant. An F(2) population (247 individuals) from the cross of S06 x S52 was used for the mapping of the Tu/tu locus. By combining bulked segregant analysis with the sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers, 15 markers (9 SRAPs and 6 SSRs) linked to the Tu/tu locus were identified. Of nine SRAP markers, three closely linked to the Tu/tu locus were successfully converted into sequence characterized amplified region (SCAR) markers. The Tu/tu locus was mapped between the co-dominant SSR marker SSR16203 and the SCAR marker C_SC933, at a genetic distance of 1.4 and 5.9 cM, respectively. Then the linked SSR markers in the study were used as anchor loci to locate the Tu/tu locus on cucumber chromosome 5. Moreover, the validity analysis of the C_SC69 and C_SC24 markers was performed with 62 cucumber lines of diverse origins, showing that the two SCAR markers can be used for marker-assisted selection (MAS) of the warty fruit trait in cucumber breeding. The information provided in this study will facilitate the map-based cloning of the Tu/tu gene.
Zhang
Weiwei
W
School of Agriculture and Biology, Shanghai Jiaotong University, Dongchuan Road, Minhang District, 200240 Shanghai, China.
He
Huanle
H
Guan
Yuan
Y
Du
Hui
H
Yuan
Lihua
L
Li
Zheng
Z
Yao
Danqing
D
Pan
Junsong
J
Cai
Run
R
D020090:Chromosome Segregation / Q000235:genetics; D018553:Cucumis sativus / Q000166:cytology / Q000235:genetics*; D005638:Fruit / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic
D005819:Genetic Markers