Association between XRCC1 polymorphisms and head and neck cancer in a Hungarian population.
BACKGROUND
Head and neck cancer is a significant current health problem in Hungary because the mortality of this cancer has increased by 387% in the last thirty-two years. Because of the important role of the XRCC1 gene in DNA repair, we wanted to test the effects of the Arg194Trp and Arg399Gln polymorphisms of XRCC1 on the clinical outcome of head and neck cancer.


METHODS
A polymerase chain reaction-restriction fragment lenght polmorphism (PCR-RFLP) method was used. A total of 108 samples were taken from intraoperatively removed formalin-fixed, and paraffin-embedded blocks of tissue. An age- and sex-matched cancer-free control group was used to compare the frequency of polymorph variants.


RESULTS
No significant difference was found between patients and controls in repect of the investigated polymorphisms. A significant difference was found between the patients with different XRCC1 194 polymorph status in clinical stage SIII. The survival proportion of patients with the Arg194Arg genotype was significantly lower than of those with the Arg194Trp genotype.


CONCLUSIONS
The complex analysis of these factors may provide the basis for personal risk assessment and an opportunity for individualised therapy.
Csejtei
Andras
A
Department of Oncoradiology, Markusovszky Hospital, Szombathely, Hungary.
Tibold
Antal
A
Koltai
Katalin
K
Varga
Zsuzsa
Z
Szanyi
Istvan
I
Gobel
Gyula
G
Prantner
Ida
I
Steffler
Denes
D
Feher
Gergely
G
De Blasio
Antonio
A
Ember
Istvan
I
Kiss
Istvan
I
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D006814:Hungary; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D015996:Survival Rate; D000076105:X-ray Repair Cross Complementing Protein 1
D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human