Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum.
Heat-shock proteins (HSP) are molecular chaperones for protein molecules. These proteins play an important role in protein-protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. A small HSP gene GHSP26 present in Gossypium arboreum responds to dehydration. In the present study, an attempt was made to overcome the problem of drought stress in cotton. A cDNA of GHSP26 was isolated from G. arboreum, cloned in plant expression vector, pCAMBIA-1301 driven by the cauliflower mosaic virus 35S promoter and introduced into Gossypium hirsutum. The integration and expression studies of putative transgenic plants were performed through GUS assay; PCR from genomic DNA, and quantitative real-time PCR analysis. Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought.
Center of Excellence in Molecular Biology, University of the Punjab, Lahore 53700, Pakistan.
Asma
A
Maqbool
Waseem
W
Abbas
Abdul Qayyum
AQ
Rao
Muhammad
M
Irfan
Muzna
M
Zahur
Allah
A
Bakhsh
Shiekh
S
Riazuddin
Tayyab
T
Husnain
D003001:Cloning, Molecular; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D003368:Gossypium / Q000235:genetics* / Q000502:physiology*; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
D050888:Heat-Shock Proteins, Small