A novel assay for influenza virus quantification using surface plasmon resonance.
Quantification of hemagglutinin (HA) by single-radial immuno diffusion (SRID) is the predominant method to ensure product potency in seasonal influenza vaccines. Here a new method for quantification of influenza virus using biosensor technology is presented. The method employs quantification of virus via an inhibition assay format using HA proteins for H1N1, H3N2 and B immobilized on a sensor chip. Initial results showed the assay to have higher sensitivity (detection range 0.5-10 microg/ml), higher precision and significantly lower analysis and hands on time compared with SRID.
GE Healthcare BioSciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden.
C Estmer
CE
Nilsson
S
S
Abbas
M
M
Bennemo
A
A
Larsson
M D
MD
Hämäläinen
A
A
Frostell-Karlsson
D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007252:Influenza Vaccines; D011786:Quality Control; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors; D019562:Viral Load; D014773:Virology / Q000379:methods*
D007252:Influenza Vaccines