Polybrominated diphenyl ethers cause oxidative stress in human umbilical vein endothelial cells.
Polybrominated diphenyl ethers (PBDEs) are used as flame retardants to prevent combustion in consumer products, such as electronics, construction materials, and textiles and, therefore, have become important commercial substances. PBDEs were also detected in maternal blood, breast milk, umbilical cord blood, and cord tissue, thereby indicating that fetuses were also exposed to PBDEs. The purpose of this study is to identify the effect of PBDEs on human umbilical vein endothelial cells (HUVECs). Cultured HUVECs were exposed to a commercial mixture of penta-BDE (DE71), octa-BDE (DE79), and deca-BDE (DE83). Each gene expression that was altered in DNA microarray was confirmed by real-time reverse transcription-polymerase chain reaction and Western blotting analysis. The results indicated that gene expressions concerning antioxidant system, i.e., thioredoxin family, 24-dehydrocholesterol reductase (DHCR24), and tumor suppressor protein p53, were altered by PBDEs exposure in HUVECs. Moreover, it was demonstrated that thioredoxin-interacting protein (TXNIP) was a target gene in exposure to DE71 and DE79 in HUVECs, by drastically decreasing time-dependent TXNIP expression in HUVECs.
Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
Yukiko
Y
Kawashiro
Hideki
H
Fukata
Koji
K
Sato
Hiroyuki
H
Aburatani
Hidetaka
H
Takigami
Chisato
C
Mori
D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013879:Thioredoxins / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects*
D017931:DNA Primers; D055768:Halogenated Diphenyl Ethers; D009419:Nerve Tissue Proteins; D016159:Tumor Suppressor Protein p53; D013879:Thioredoxins; D044925:Oxidoreductases Acting on CH-CH Group Donors; C499055:DHCR24 protein, human