Contribution of microextraction in packed sorbent for the analysis of cotinine in human urine by GC-MS.
A simple, rapid, sensitive, and non-consuming solvent method for the determination of cotinine in urine was developed, based on sample preparation by the relatively new technique microextraction in packed sorbent (MEPS) and analysis by GC-MS. This optimized method was compared with conventional solid-phase extraction/liquid-liquid extraction method used as reference. The wide linear range (5-5,000 ng/mL) and high sensitivity of the MEPS method (limit of detection 0.8 ng/mL) allow application to analysis of urine from smokers as well as non-smokers susceptible to passive smoking.
CNRS Service Central d'Analyse-USR59, Chemin du Canal, 69360 Solaize, France.
Florent
F
Lafay
Emmanuelle
E
Vulliet
Marie-Magdeleine
MM
Flament-Waton
D003367:Cotinine / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
D003367:Cotinine