[Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
This study was purposed to investigate the practicality of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for detection of single nucleotide polymorphisms (SNP) on jak2 gene in multiple myeloma (MM) cells. The DNA fragment containing 2 SNPs of jak2 (C428T and C643T) was amplified using PCR and was purified. The purified product was used as the template for primer extension (PEX). The small products of allele-specific reaction were purified, the SNPs on jak2 gene of 5 patients with MM and 5 healthy persons were detected by MALDI-TOF MS. The results showed that the distribution of genotype C428T and C643T was not different between MM patients and healthy persons, both of which are homozygous T/T. In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice.
Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College, Nanjing 210009, Jiangsu Province, China.
Yue-Jiao
YJ
Zhoang
Bao-An
BA
Chen
Lu
L
Cheng
Ji-Feng
JF
Feng
Yu-Feng
YF
Li
Jun
J
Qian
Jia-Hua
JH
Ding
Jian
J
Cheng
Feng
F
Gao
Guo-Hua
GH
Xia
Ning
N
Sun
Yan
Y
Zhang
Xiao-Ping
XP
Zhang
Pei-Pei
PP
Xu
Zu-Hong
ZH
Lu
D016022:Case-Control Studies; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D017931:DNA Primers; C507924:JAK2 protein, human; D053614:Janus Kinase 2