Cloning and functional analysis of an allene oxide synthase in Physcomitrella patens.
Jasmonic acid (JA) is a plant hormone that plays important roles in a large number of processes in stress adaptation and development in flowering plants. A search of genome database indicated the existence of allene oxide synthase (AOS), an enzyme of JA biosynthesis, in Physcomitrella patens, a model plant among mosses. In this study, the presence of JA was detected in P. patens. The recombinant AOS of P. patens, which was overexpressed in Escherichia coli, showed AOS activity. These data suggest that the octadecanoid pathway also exists in P. patens.
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
P K G S S
PK
Bandara
Kosaku
K
Takahashi
Michio
M
Sato
Hideyuki
H
Matsuura
Kensuke
K
Nabeta
D000595:Amino Acid Sequence; D019068:Bryopsida / Q000201:enzymology*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism; D016415:Sequence Alignment
D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase