Coordinated expression of MMPs and TIMPs in rat knee intra-articular tissues after ACL injury.
Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a rat ACL rotating injury model, we found that after ACL injury, the mRNA levels of MMP-13, TIMP-1, and CD147 were significantly elevated in ACL, posterior cruciate ligament (PCL), synovium, meniscus, and cartilage. Also, MMP-2 activity was also elevated significantly in a time-dependent manner in all intra-articular tissues. Synovium showed the most capability to release MMPs, whereas ACL showed the highest MMP-13/TIMP-1 ratio. Generic MMP activity assay and zymography showed time dependent elevation of MMP activities in synovial fluids (SF). We concluded that the ACL injury would induce a coordinated response of intra-articular tissues to express MMPs, TIMPs, and CD147. The MMP activities in the microenvironment in SF would accumulate, released by all the intra-articular tissues, which would contribute to the knee damage and degeneration induced by ACL injury.
Tang
Zhenyu
Z
Chongqing University, China.
Yang
Li
L
Zhang
Jin
J
Xue
Ruyue
R
Wang
Yequan
Y
Chen
Peter C
PC
Sung
K-L Paul
KL
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000201:enzymology* / Q000503:physiopathology; D000070598:Anterior Cruciate Ligament Injuries; D051926:Basigin / Q000235:genetics / Q000378:metabolism; D002358:Cartilage, Articular / Q000201:enzymology; D004195:Disease Models, Animal; D018450:Disease Progression; D057075:Enzyme Assays; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007593:Joint Instability / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology; D007718:Knee Injuries / Q000201:enzymology* / Q000503:physiopathology; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008592:Menisci, Tibial / Q000201:enzymology; D016119:Posterior Cruciate Ligament / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000201:enzymology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D051926:Basigin; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2