[Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
This study was purposed to investigate the practicality of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for detection of single nucleotide polymorphisms (SNP) on jak2 gene in multiple myeloma (MM) cells. The DNA fragment containing 2 SNPs of jak2 (C428T and C643T) was amplified using PCR and was purified. The purified product was used as the template for primer extension (PEX). The small products of allele-specific reaction were purified, the SNPs on jak2 gene of 5 patients with MM and 5 healthy persons were detected by MALDI-TOF MS. The results showed that the distribution of genotype C428T and C643T was not different between MM patients and healthy persons, both of which are homozygous T/T. In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice.
Zhoang
Yue-Jiao
YJ
Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College, Nanjing 210009, Jiangsu Province, China.
Chen
Bao-An
BA
Cheng
Lu
L
Feng
Ji-Feng
JF
Li
Yu-Feng
YF
Qian
Jun
J
Ding
Jia-Hua
JH
Cheng
Jian
J
Gao
Feng
F
Xia
Guo-Hua
GH
Sun
Ning
N
Zhang
Yan
Y
Zhang
Xiao-Ping
XP
Xu
Pei-Pei
PP
Lu
Zu-Hong
ZH
D016022:Case-Control Studies; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D017931:DNA Primers; C507924:JAK2 protein, human; D053614:Janus Kinase 2