Isolation and antifungal susceptibility of Exophiala dermatitidis isolates from human stool samples in Nigeria.
We screened a total of 460 stool samples from humans with and without underlying disease for the black yeast and neurotrope, Exophiala dermatitidis using conventional and molecular identification procedures. Sixteen (3.5%) samples were positive. Twelve out of the sixteen positive samples where the fungus was recovered were from individuals who had diarrhoea at the moment of isolation. Two asymptomatic positive cases were within the age bracket of 21-30. Susceptibility testing of the strains using the CLSI M38 protocol suggests that almost all the strains were susceptible to amphotericin B, 5 fluorocytosine, itraconazole, fluconazole and voriconazole. Considering the health risk currently posed by this organism in Asia, especially among healthy younger subjects of the population and the scarcity of data on this fungus in Africa in general and Nigeria in particular, some recommendations were suggested.
Nweze
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Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria. nwezemeka@yahoo.com
Ezute
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D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002353:Carrier State / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D005093:Exophiala / Q000187:drug effects* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009181:Mycoses / Q000382:microbiology*; D009549:Nigeria; D055815:Young Adult
D000935:Antifungal Agents