Effect and mechanism of AR-6 in experimental rheumatoid arthritis.
The root of Clematis chinensis Osbeck has been used widely in rheumatoid arthritis in Chinese traditional medicine and AR-6 is a triterpene saponin isolated from it. In this present study, we investigated in vivo effects of oral AR-6 in chronic rat adjuvant-induced arthritis (AA) and in vitro effect in macrophage and synoviocytes cells. Arthritic scores and serum inflammatory mediators were evaluated 19 days after AA induction by endermic injection of Freund's complete adjuvant in Sprague-Dawley(S-D) rats. Oral administration of AR-6 to arthritic rats resulted in a clear decrease of clinical signs compared to untreated controls. The synoviocyte and macrophage response ex vivo were then analyzed. Anti-arthritic effects of AR-6 correlated with significant decrease of NO and TNF-alpha produced by peritoneal macrophages, ex vivo and in vitro. AR-6 also significant decreased the proliferation of synoviocyte. These data indicate that AR-6 is a potential anti-inflammatory therapeutic and preventive agent.
Sun
Shi-xue
SX
Department of Physiology, China Pharmaceutical University, 210009 Nanjing, China.
Li
Yun-man
YM
Fang
Wei-rong
WR
Cheng
Peng
P
Liu
Lifang
L
Li
Fengwen
F
D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D018836:Inflammation Mediators / Q000097:blood; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage*; D012720:Severity of Illness Index; D013582:Synovial Fluid / Q000166:cytology; D016896:Treatment Outcome; D014315:Triterpenes / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D012503:Saponins; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide