Effect of ploidy levels on the activities of delta(1)-pyrroline-5-carboxylate synthetase, superoxide dismutase and peroxidase in Cenchrus species grown under water stress.
The effects of ploidy levels on the activities of delta(1)-pyrroline-5-carboxylate synthetase (P5CS; EC not assigned), superoxide dismutase (SOD; EC 1.15.1.1) and guaiacol peroxidase (POX; EC 1.11.1.7), as well as malondialdehyde (MDA) and proline contents were studied in two months old plants of Cenchrus species. The Cenchrus species represent three ploidy levels: diploid, tetraploid, hexaploid and two life spans: annual and perennial. Plants were subjected to water stress for 2, 4, 6 and 8 d by withholding water under glasshouse conditions. Although the levels of proline increased with the magnitude of water stress, the P5CS activity did not show a corresponding increase in all species. Peroxidase and superoxide dismutase activities showed an increase or steady state in the early phase of drought and then declined with the further increase in the magnitude of water stress, indicating differing behaviors of species towards drought tolerance. Under drought, diploid Cenchrus species had a higher POX activity, MDA accumulation and lower proline content than tetraploid species. Lower POX and higher P5CS activities and proline contents, however, were observed in hexaploid and tetraploid species. Taken together, our findings suggest that diploid species have a less efficient antioxidant system to scavenge reactive oxygen species than tetra and hexaploid Cenchrus. This may result in a corresponding variability in growth and persistence under natural grasslands. The study also paves the way for investigations on the molecular events associated with drought in Cenchrus species differing in ploidy and life span.
Plant Physiology and Biochemistry Laboratory, Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi 284003, UP, India. amaresh62@yahoo.com
Amaresh
A
Chandra
Archana
A
Dubey
D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D031741:Cenchrus / Q000201:enzymology / Q000235:genetics*; D002875:Chromosomes; D003681:Dehydration; D003890:Desiccation; D055864:Droughts; D017343:Genes, Plant; D050698:Glutamate-5-Semialdehyde Dehydrogenase / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011123:Polyploidy; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; D009097:Multienzyme Complexes; D017382:Reactive Oxygen Species; C549031:delta(1)-pyrroline-5-carboxylate synthetase, Cenchrus; D008315:Malondialdehyde; D011392:Proline; C121048:guaiacol peroxidase; D009195:Peroxidase; D013482:Superoxide Dismutase; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); D008025:Ligases