Contribution of microbial activity to carbon chemistry in clouds.
The biodegradation of the most abundant atmospheric organic C1 to C4 compounds (formate, acetate, lactate, succinate) by five selected representative microbial strains (three Pseudomonas strains, one Sphingomonas strain, and one yeast strain) isolated from cloud water at the puy de Dôme has been studied. Experiments were first conducted under model conditions and consisted of a pure strain incubated in the presence of a single organic compound. Kinetics showed the ability of the isolates to degrade atmospheric compounds at temperatures representative of low-altitude clouds (5 degrees C and 17 degrees C). Then, to provide data that can be extrapolated to real situations, microcosm experiments were developed. A solution that chemically mimicked the composition of cloud water was used as an incubation medium for microbial strains. Under these conditions, we determined that microbial activity would significantly contribute to the degradation of formate, acetate, and succinate in cloud water at 5 degrees C and 17 degrees C, with lifetimes of 0.4 to 69.1 days. Compared with the reactivity involving free radicals, our results suggest that biological activity drives the oxidation of carbonaceous compounds during the night (90 to 99%), while its contribution accounts for 2 to 37% of the reactivity during the day, competing with photochemistry.
Clermont Université, Laboratoire SEESIB, Clermont-Ferrand, France.
Mickaël
M
Vaïtilingom
Pierre
P
Amato
Martine
M
Sancelme
Paolo
P
Laj
Maud
M
Leriche
Anne-Marie
AM
Delort
D000391:Air Microbiology; D001272:Atmosphere / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D020578:Sphingomonas / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D015003:Yeasts / Q000302:isolation & purification / Q000378:metabolism*
D002244:Carbon