Double-stranded RNA poly(I:C) enhances matrix metalloproteinase mRNA expression in human nasal polyp epithelial cells.
CONCLUSIONS
The significant up-regulation of matrix metalloproteinase (MMP)-9 mRNA, which is not modulated by tissue inhibitor of metalloproteinase (TIMP)-1, is an additional source of increased proteolytic activity in virus-infected upper airways that might contribute to the exacerbation of chronic rhinosinusitis with nasal polyps.


OBJECTIVE
Chronic rhinosinusitis is often exacerbated by viral infection. We hypothesized that a disruption of the mechanisms that regulate the activity of MMPs during viral infection is one possible mechanism responsible for the exacerbation. In the present study we attempted to achieve a better understanding of MMP expression in nasal epithelial cells after viral infection.


METHODS
Human nasal epithelial cells were isolated from nasal polyp specimens obtained during endoscopic endonasal surgery in chronic rhinosinusitis patients. We investigated the expression of MMP-2, MMP-9, and TIMP-1 mRNA in primary human nasal polyp epithelial cells after dsRNA stimulation.


RESULTS
Among the genes whose expression was evaluated, only expression of MMP-9 mRNA increased significantly after dsRNA stimulation.
Department of Otorhinolaryngology, Showa University School of Medicine, Tokyo, Japan.
Jiyun
J
Wang
So
S
Watanabe
Satoshi
S
Matsukura
Harumi
H
Suzaki
D000998:Antiviral Agents / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism*; D009298:Nasal Polyps / Q000473:pathology*; D011070:Poly I-C / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012220:Rhinitis / Q000821:virology; D012852:Sinusitis / Q000821:virology; D015854:Up-Regulation
D000998:Antiviral Agents; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D020782:Matrix Metalloproteinases; D011070:Poly I-C