Dynamic aberration correction for multiharmonic microscopy.
We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2(2N+1) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, F-91128 Palaiseau, France.
Nicolas
N
Olivier
Delphine
D
Débarre
Emmanuel
E
Beaurepaire
D000465:Algorithms; D000818:Animals; D015509:Developmental Biology / Q000379:methods; D004330:Drosophila / Q000196:embryology*; D004867:Equipment Design / Q000379:methods; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055095:Optics and Photonics; D012212:Rheology / Q000379:methods; D013997:Time Factors