Plumieribetin, a fish lectin homologous to mannose-binding B-type lectins, inhibits the collagen-binding alpha1beta1 integrin.
Recently, a few fish proteins have been described with a high homology to B-type lectins of monocotyledonous plants. Because of their mannose binding activity, they have been ascribed a role in innate immunity. By screening various fish venoms for their integrin inhibitory activity, we isolated a homologous protein from the fin stings and skin mucus of the scorpionfish (Scorpaena plumieri). This protein inhibits alpha1beta1 integrin binding to basement membrane collagen IV. By protein chemical and spectroscopic means, we demonstrated that this fish protein, called plumieribetin, is a homotetramer and contains a high content of anti-parallel beta strands, similar to the mannose-binding monocot B-lectins. It lacks both N-linked glycoconjugates and common O-glycan motifs. Despite its B-lectin-like structure, plumieribetin binds to alpha1beta1 integrin irrespective of N-glycosylation, suggesting a direct protein-protein interaction. This interaction is independent of divalent cations. On the cellular level, plumieribetin failed to completely detach hepatocarcinoma HepG2 cells and primary arterial smooth muscle cells from the collagen IV fragment CB3. However, plumieribetin weakened the cell-collagen contacts, reduced cell spreading, and altered the actin cytoskeleton, after the compensating alpha2beta1 integrin was blocked. The integrin inhibiting effect of plumieribetin adds a new function to the B-lectin family, which is known for pathogen defense.
de Santana Evangelista
Karla
K
Center for Molecular Medicine, Department of Vascular Matrix Biology, Frankfurt University Hospital, Excellence Cluster Cardio-Pulmonary System, 60590 Frankfurt, Germany.
Andrich
Filipe
F
Figueiredo de Rezende
Flávia
F
Niland
Stephan
S
Cordeiro
Marta N
MN
Horlacher
Tim
T
Castelli
Riccardo
R
Schmidt-Hederich
Alletta
A
Seeberger
Peter H
PH
Sanchez
Eladio F
EF
Richardson
Michael
M
Gomes de Figueiredo
Suely
S
Eble
Johannes A
JA
D000595:Amino Acid Sequence; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D024141:Collagen Type IV / Q000378:metabolism*; D005399:Fishes; D006801:Humans; D039222:Integrin alpha1beta1 / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D014688:Venoms / Q000737:chemistry
D024141:Collagen Type IV; D039222:Integrin alpha1beta1; D037102:Lectins; D014688:Venoms