Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description.
The species Aeromonas simiae was first described on the basis of two strains (CIP 107798(T) and CIP 107797) isolated from faeces of healthy monkeys (M. fascicularis) from Mauritius, which were kept in quarantine in the Centre for Primatology, Louis Pasteur University, Strasbourg, France. In the present study, during a survey to determine the prevalence of Aeromonas at three different pig slaughterhouses at north of Portugal, a single strain MDC2374 (out of 703 isolates) was identified as A. simiae on the basis of 16S rDNA, gyrB and rpoD sequencing, confirming that traditional biochemical approaches are not resolute to identify rarely isolated Aeromonas species. To our knowledge, this is the first time since species description that A. simiae is newly isolated and identified.
Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.
Maria Conceição
MC
Fontes
Maria José
MJ
Saavedra
Arturo
A
Monera
Conceição
C
Martins
Antonio
A
Martínez-Murcia
D000003:Abattoirs; D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D027081:DNA Gyrase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase