Histamine stimulates human lung fibroblast migration.
Histamine is a potent mediator in allergic inflammatory processes and is released by basophils and mast cells. The aim of this study was to investigate the effect of histamine on in vitro migration of human fetal lung fibroblasts (HFL-1) to human plasma fibronectin (HFn), a chemoattractant. Using the blindwell chamber technique, histamine alone had no chemotactic activity. However, histamine augmented HFn-induced HFL-1 migration at concentrations ranging between 0 and 10(-7) M (290.6 +/- 20.8%) (P < 0.05). The concentration-response was bell-shaped. The effect of histamine increased with time. The stimulatory effect of histamine on HFL-1 migration was inhibited by an H4 receptor antagonist, JNJ7777120 (10(-5) M). Histamine's effect was also inhibited by pertussis toxin (50 ng/ml), showing that the effect was mediated by the H4 receptor. This study demonstrated that histamine has the potential to stimulate human lung fibroblast migration, and thus may contribute to regulation of wound healing and the development of fibrotic disorders of the lung.
Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. koyama-tky@umin.ac.jp
Tadashi
T
Kohyama
Yasuhiro
Y
Yamauchi
Hajime
H
Takizawa
Sumiko
S
Kamitani
Shin
S
Kawasaki
Takahide
T
Nagase
D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D005353:Fibronectins / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008168:Lung / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D011968:Receptors, Histamine / Q000378:metabolism / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects
D005353:Fibronectins; D006633:Histamine Antagonists; D007211:Indoles; D010879:Piperazines; D011968:Receptors, Histamine; C484309:1-((5-chloro-1H-indol-2-yl)carbonyl)-4-methylpiperazine; D006632:Histamine; D011899:Ranitidine; D004155:Diphenhydramine