Determination of urinary adenosine using resonance light scattering of gold nanoparticles modified structure-switching aptamer.
A novel sensitive method has been developed for the detection of adenosine (AD) in human urine by using enhanced resonance light scattering (RLS). This method is based on the specific recognition and signal amplification of adenosine aptamer (Apt) coupled with gold nanoparticles (GNPs) via G-quartet-induced nanoparticle assembly, which was fabricated by triggering a structure switching of the 3' terminus G-rich sequence and aptamer duplex. RLS signal linearly correlated with the concentration of adenosine over the range of 6-115nM. The limit of detection (LOD) for adenosine is 1.8nM with relative standard deviations (RSD) of 2.90-4.80% (n=6). The present method has been successfully applied to determination of adenosine in real human urine, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the combination of the excellent selectivity of aptamer with the high sensitivity of the RLS technique could provide a promising potential for aptamer-based small molecule detection, and be beneficial in extending the application of RLS.
Zhang
Jin-Quan
JQ
College of Public Health, University of South China, Hengyang 421001, People's Republic of China.
Wang
Yong-Sheng
YS
He
Yan
Y
Jiang
Tao
T
Yang
Hong-Mei
HM
Tan
Xuan
X
Kang
Rong-Hui
RH
Yuan
Yu-Kun
YK
Shi
Lin-Fei
LF
D000241:Adenosine / Q000652:urine*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013696:Temperature
D052157:Aptamers, Nucleotide; D006046:Gold; D000241:Adenosine