Cytotoxic activity and G1 cell cycle arrest of a Dienynone from Echinacea pallida.
In the present study, a further investigation of the cytotoxic activity of an acetylenic constituent of Echinacea pallida roots, namely, pentadeca-(8 Z,13 Z)-dien-11-yn-2-one, was performed, revealing a concentration-dependent cytotoxicity on several human cancer cell lines, including leukemia (Jurkat and HL-60), breast carcinoma (MCF-7), and melanoma (MeWo) cells. As part of its mechanism of action, the ability of this constituent to arrest the cell cycle in the G1 phase was demonstrated on HL-60 cells. Furthermore, a stability test of the target compound over 72 h was carried out, indicating that the cytotoxic activity can be attributed mainly to the genuine, not oxidized, molecule.
Chicca
Andrea
A
Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Pisa, Italy. andrea.chicca@ittumori.it
Adinolfi
Barbara
B
Pellati
Federica
F
Orlandini
Giulia
G
Benvenuti
Stefania
S
Nieri
Paola
P
D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D020900:Echinacea; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry
D000970:Antineoplastic Agents; D007659:Ketones; D010936:Plant Extracts; C549757:pentadeca-(8Z,13Z)-dien-11-yn-2-one