Parvovirus PARV4 visualization and detection.
The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5 x 10(8) genome equivalents ml(-1). Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.
Tuke
Philip W
PW
Health Protection Agency, Centre for Infections, Virus Reference Department, 61 Colindale Avenue, London NW9 5HT, UK.
Parry
Ruth P
RP
Appleton
Hazel
H
D000914:Antibodies, Viral / Q000097:blood; D006801:Humans; D008854:Microscopy, Electron; D010322:Parvoviridae Infections / Q000276:immunology / Q000821:virology*; D017992:Parvovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure*; D019562:Viral Load; D014771:Virion / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure
D000914:Antibodies, Viral