Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein.
Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 A. The crystals belonged to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 118.8, b = 118.8, c = 495.1 A, alpha = beta = 90, gamma = 120 degrees .
School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore. masayo@hku.hk
Masayo
M
Kotaka
Sujit
S
Dutta
Hooi Chen
HC
Lee
Mitchell J M
MJ
Lim
Yeehwa
Y
Wong
Feng
F
Rao
Edward P
EP
Mitchell
Zhao Xun
ZX
Liang
Julien
J
Lescar
D003460:Crystallization; D018360:Crystallography, X-Ray; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
D014157:Transcription Factors