Using LC3 to monitor autophagy flux in the retinal pigment epithelium.
Autophagy is a highly conserved housekeeping pathway that plays a critical role in the removal of aged or damaged intracellular organelles and their delivery to lysosomes for degradation. Autophagy begins with the formation of membranes, arising in part from the endoplasmic reticulum, that elongate and fuse engulfing cytoplasmic constituents into a classic double-membrane bound nascent autophagosome. These early autophagosomes undergo a stepwise maturation process to form the late autophagosome or amphisome that ultimately fuses with a lysosome. Efficient autophagy is dependent on an equilibrium between the formation and elimination of autophagosomes; thus, a deficit in any part of this pathway will cause autophagic dysfunction. Autophagy plays a role in aging and age-related diseases. However, few studies of autophagy in retinal disease have been reported.
Wang
Ai Ling
AL
Forsythe Laboratory for the Investigation of the Aging Retina, Department of Ophthalmology, Northwestern University School of Medicine, Chicago, IL, USA. alwang0526@yahoo.com
Boulton
Michael E
ME
Dunn
William A
WA
Rao
Harripriya Vittal
HV
Cai
Jun
J
Lukas
Thomas J
TJ
Neufeld
Arthur H
AH
D001343:Autophagy; D015153:Blotting, Western; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection
C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D049452:Green Fluorescent Proteins