Ischemia-reperfusion alters cardiac lipoprotein lipase.
Ischemia-reperfusion (I/R) is associated with changes in energy metabolism in the heart. However, the majority of studies have focused on examining rates and extent of fatty acid (FA) oxidation, with limited emphasis on FA delivery. We examined the influence of acute myocardial I/R on coronary lipoprotein lipase (LPL), the key enzyme responsible for triglyceride-lipoprotein hydrolysis and FA delivery to the heart. In a whole animal and an ex vivo model of I/R, we demonstrate increases in luminal LPL activity, an effect that involved signaling through nitric oxide. Given the damaging effect of excess FA utilization by the ischemic heart, strategies to restrict LPL at the vascular lumen would be an attractive therapeutic option in limiting I/R related cardiac injury.
Cardiovascular Research Center, Department of Pediatrics, 430, Heritage Medical Research Centre, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G2S2, Canada.
Thomas
T
Pulinilkunnil
Prasanth
P
Puthanveetil
Min Suk
MS
Kim
Fang
F
Wang
Veronika
V
Schmitt
Brian
B
Rodrigues
D000818:Animals; D015153:Blotting, Western; D005227:Fatty Acids / Q000378:metabolism; D006321:Heart / Q000502:physiology*; D007511:Ischemia / Q000201:enzymology*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D015425:Myocardial Reperfusion; D032383:Myocytes, Cardiac / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D008074:Lipoproteins; D014280:Triglycerides; C030087:lipoprotein triglyceride; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D008071:Lipoprotein Lipase