A novel splice variant of FR4 predominantly expressed in CD4+CD25+ regulatory T cells.
Folate receptor 4 (FR4) is recently found as a lymphoid tissue specific protein. In this study, we have identified an alternative splicing variant of the FR4 gene from murine splenocytes, termed FR4v, which is almost identical to FR4 cDNA sequence except with the retained 108 bp intron 3 between exon 3 and 4 of FR4 gene. FR4v mRNA encodes a larger protein than FR4 and is constitutively expressed on CD4(+)CD25(+) regulatory T cell (Treg) membrane via a GPI anchor mechanism. Whether FR4v plays a redundant or unique functional role in Tregs should be investigated further in the future.
Jia
Zhengcai
Z
Institute of Immunology, PLA, Third Military Medical University, Chongqing, 400038, PR China.
Zhao
Ren
R
Tian
Yi
Y
Huang
Zemin
Z
Tian
Zhiqiang
Z
Shen
Zhigang
Z
Wang
Qinghong
Q
Wang
Jingxue
J
Fu
Xiaolan
X
Wu
Yuzhang
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis; D002469:Cell Separation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D005434:Flow Cytometry; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D007438:Introns / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013154:Spleen; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*
D015704:CD4 Antigens; D017261:Glycosylphosphatidylinositols; D053645:Interleukin-2 Receptor alpha Subunit; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; C543634:folate receptor 4, mouse