Monoglyceride and diglyceride production through lipase-catalyzed glycerolysis and molecular distillation.
Distilled glycerides are obtained through distillation of the system mono-diglycerides which is produced from the esterification reaction between a triglyceride with glycerol. In this work, monoglycerides (MG) and diglycerides (DG) are produced through lipase-catalyzed glycerolysis of soybean oil using Candida antarctica B in a solvent-free system. To separate the products of the reaction in order to obtain essentially MG and an oil of DG, it is necessary to use a suitable process in order to preserve the stability of the components and to keep the products free of inappropriate solvents. So, after 24 h of enzymatic reaction, the mixture of acylglycerols and fatty acids was distilled into a centrifugal molecular distiller, since it provides a free solvent and lower temperature environment to increase the desired product concentration. Starting from a material with 25.06% of triglycerides (TG), 46.63% of DG, 21.72% of MG, 5.38% of free fatty acids (FFA), and 1.21% of glycerol, the MG purity in the distillate stream was 80% at evaporator temperature (T (E)) equal to 250 degrees C and feed flow rate (Q) equal to 10.0 mL/min. At these conditions, the MG recovery was 35%. The material collected in the residue stream presented DG-enriched oil with TG unhydrolyzed, residual MG, and low acidity (29.83% of TG, 53.20% of DG, 15.64% of MG, and 1.33% of FFA), which is suitable to replace TG oil in the human diet.
Separation Process Development Laboratory, Chemical Engineering School, State University of Campinas, 13081970 Campinas, São Paulo, Brazil. lucente@feq.unicamp.br
Patricia Bogalhos Lucente
PB
Fregolente
Gláucia Maria F
GM
Pinto
Maria Regina
MR
Wolf-Maciel
Rubens Maciel
RM
Filho
D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D002498:Centrifugation / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005656:Fungal Proteins; D005990:Glycerol / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D013024:Soybean Oil / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D050178:Monoglycerides; D013024:Soybean Oil; D008049:Lipase; C431349:lipase B, Candida antarctica; D005990:Glycerol