Supercritical phase inversion of starch-poly(epsilon-caprolactone) for tissue engineering applications.
In this work, a starch-based polymer, namely a blend of starch-poly(epsilon-caprolactone) was processed by supercritical assisted phase inversion process. This processing technique has been proposed for the development of 3D structures with potential applications in tissue engineering applications, as scaffolds. The use of carbon dioxide as non-solvent in the phase inversion process leads to the formation of a porous and interconnected structure, dry and free of any residual solvent. Different processing conditions such as pressure (from 80 up to 150 bar) and temperature (45 and 55 degrees C) were studied and the effect on the morphological features of the scaffolds was evaluated by scanning electron microscopy and micro-computed tomography. The mechanical properties of the SPCL scaffolds prepared were also studied. Additionally, in this work, the in vitro biological performance of the scaffolds was studied. Cell adhesion and morphology, viability and proliferation was assessed and the results suggest that the materials prepared are allow cell attachment and promote cell proliferation having thus potential to be used in some for biomedical applications.
Department of Polymer Engineering, 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909, Caldas das Taipas, Guimarães, Portugal. aduarte@dep.uminho.pt
Ana Rita C
AR
Duarte
João F
JF
Mano
Rui L
RL
Reis
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D019245:Compressive Strength; D003460:Crystallization / Q000379:methods; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D044367:Phase Transition; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D011312:Pressure; D013213:Starch / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D013213:Starch