Modulatory role of alizarin from Rubia cordifolia L. against genotoxicity of mutagens.
Rubia cordifolia L. (Rubiaceae) is an important medicinal plant used in the Ayurvedic medicinal system. Its use as a traditional therapeutic has been related to the treatment of skin disorders and cancer. Besides its medicinal value, anthraquinones from this plant are used as natural food colourants and as natural hair dyes. Dyes derived from natural sources have emerged as important alternatives to synthetic dyes. Alizarin (1,2-dihydroxyanthraquinone) was isolated and characterized from R. cordifolia L. and evaluated for its antigenotoxic potential against a battery of mutagens viz. 4-nitro-o-phenylenediamine (NPD) and 2-aminofluorene (2-AF) in Ames assay using TA98 tester strain of Salmonella typhimurium; hydrogen peroxide (H(2)O(2)) and 4-nitroquinoline-1-oxide (4NQO) in SOS chromotest using PQ37 strain of Escherichia coli and in Comet assay using human blood lymphocytes. Our results showed that alizarin possessed significant modulatory role against the genotoxicity of mutagens.
Kaur
Prabhjit
P
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Chandel
Madhu
M
Kumar
Subodh
S
Kumar
Neeraj
N
Singh
Bikram
B
Kaur
Satwinderjeet
S
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000880:Anthraquinones / Q000494:pharmacology*; D016587:Antimutagenic Agents; D002396:Catechols / Q000633:toxicity; D002855:Chromatography, Thin Layer; D020552:Comet Assay; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005449:Fluorenes / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity; D018517:Plant Roots / Q000737:chemistry; D015363:Quinolones / Q000633:toxicity; D045470:Rubia / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
C120195:4-nitroquinolone-1-oxide; D000880:Anthraquinones; D016587:Antimutagenic Agents; D002396:Catechols; D005449:Fluorenes; D009153:Mutagens; D009942:Organometallic Compounds; D016877:Oxidants; D015363:Quinolones; C042235:neodymium pyrocatechin disulfonate; C012177:2-aminofluorene; D015112:4-Nitroquinoline-1-oxide; C010078:alizarin; D006861:Hydrogen Peroxide