Evaluation of the effects of endodontic materials on fibroblast viability and cytokine production.
BACKGROUND
Recently, a new sealer composed of Portland cement named Endo-CPM-Sealer was developed. The aim of this study was to investigate the effects of Endo-CPM-Sealer (EGEO SRL, Buenos Aires, Argentina), Sealapex (Sybron Endo, Glendora, CA), and Angelus MTA (Angelus, Londrina, Brazil) on cell viability and cytokine (interleukin [IL]-1beta and IL-6) production by mouse fibroblasts.


METHODS
Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts. Cells cultured with only empty polyethylene tubes were used as the control. After 24 hours, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to evaluate the cell viability. For cytokine assay, mouse fibroblasts were incubated in 24-well flat-bottom plates with set material disks at the bottom. Cells cultured without the material disks served as the negative control. After 24 hours of incubation, culture media were collected for cytokine evaluation by using an enzyme-linked immunosorbent assay. The data were statistically analyzed by analysis of variance and Bonferroni correction.


RESULTS
Endo-CPM-Sealer, Sealapex, and Angelus MTA did not inhibit the cell viability. All materials induced IL-6 releasing, but the amount was not statistically significant compared with the control group. Angelus MTA induced IL-1beta releasing significantly more than the control.


CONCLUSIONS
All materials were not considered cytotoxic in fibroblast culture.
Department of Endodontics, Araçatuba School of Dentistry, São Paulo State University, São Paulo, Brazil. joao@foa.unesp.br
João Eduardo
JE
Gomes-Filho
Simone
S
Watanabe
Alessandra Cristina
AC
Gomes
Max Douglas
MD
Faria
Carolina Simonetti
CS
Lodi
Sandra Helena
SH
Penha Oliveira
D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D053583:Interleukin-1beta / Q000187:drug effects; D015850:Interleukin-6 / Q000032:analysis; D007378:Interleukins / Q000032:analysis*; D008422:Materials Testing; D051379:Mice; D010087:Oxides / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D017640:Silicates / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; C540763:Endo-CPM-Sealer; D053583:Interleukin-1beta; D015850:Interleukin-6; D007378:Interleukins; D010087:Oxides; D012387:Root Canal Filling Materials; D012459:Salicylates; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; C055601:Sealapex; C022616:thiazolyl blue; D002126:Calcium Hydroxide