Expression of transient receptor potential vanilloid (TRPV) families 1, 2, 3 and 4 in the mouse olfactory epithelium.
We investigated the expression of transient receptor potential vanilloid (TRPV)-1-4 in the mouse olfactory epithelium (OE) in comparison to its expression in respiratory epithelium. CBA/J mice were used. The localization of TRPV-1, -2, -3 and -4 in the nasal mucosa was investigated using immunohistochemistry and a double staining study for TRPV-1 and -2 and SP was also carried out. TRPV-1-4, were expressed variably in the OE with a diffuse pattern in lamina propria, and were expressed in respiratory epithelium with strong positive expression in glandular cells of lamina propria. The double-staining study revealed coexpression of TRPV-1 and -2 and substance P (SP) in the trigeminal nerve fibers of the OE. Coexpression of TRPV-1 and SP was marked around the blood vessels and seromucinous gland of respiratory epithelium while TRPV-2 showed no co-localization. TRPV-1-4 were found to be localized in the mouse OE and respiratory epithelium. Our results suggest that TRPVs may play multiple roles in the OE, contributing to olfactory adaptation, olfactory/trigeminal interactions in nasal chemoreception and OE homeostasis; they may also be involved in olfactory transduction as well as olfactory dysfunction secondary to sinonasal inflammatory disease. TRPVs in respiratory mucosa may play a significant role in nasal nociception, ciliary movement and the regulation of mucous secretion.
Department of Otorhinolaryngology, Head & Neck Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima city, Japan.
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Khalifa Ahmed
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Takumida
Department of Otorhinolaryngology, Head & Neck Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima city, Japan.
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Ishibashi
Department of Otorhinolaryngology, Head & Neck Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima city, Japan.
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Hamamoto
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Hirakawa
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D009297:Nasal Mucosa / Q000378:metabolism; D009831:Olfactory Mucosa / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism*
D015220:Calcium Channels; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C485717:Trpv2 protein, mouse; C486760:Trpv3 protein, mouse; C486576:Trpv4 protein, mouse