MicroRNA-214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells.
MicroRNAs are a group of endogenously expressed, single-stranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
Zuozhen
Z
Yang
Shuang
S
Chen
Xuejing
X
Luan
Yixuan
Y
Li
Min
M
Liu
Xin
X
Li
Tao
T
Liu
Hua
H
Tang
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D048371:MAP Kinase Kinase 3 / Q000096:biosynthesis; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D008875:Middle Aged; D048055:Mitogen-Activated Protein Kinase 8 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D048055:Mitogen-Activated Protein Kinase 8; D048371:MAP Kinase Kinase 3; C482530:MAP2K3 protein, human