Anti-human proacrosin antibody inhibits the zona pellucida (ZP)-induced acrosome reaction of ZP-bound spermatozoa.
The anti-acrosin monoclonal antibody AcrC5F10 inhibited proacrosin activation, proacrosin-human zona pellucida glycoprotein A (ZPA) binding, and the zona pellucida (ZP)-induced acrosome reaction of the ZP-bound spermatozoa but had no significant effect on sperm-ZP binding. These results suggest that proacrosin-acrosin may play an important role in the ZP-induced acrosome reaction of spermatozoa after primary binding to the ZP.
Instituto de Biología y Medicina Experimental (IBYME), National Research Council of Argentina (CONICET), 1428ADN, Buenos Aires, Argentina.
Carolina
C
Veaute
De Yi
DY
Liu
Laura I
LI
Furlong
Juan C
JC
Biancotti
H W G
HW
Baker
Mónica H
MH
Vazquez-Levin
D000176:Acrosin / Q000276:immunology* / Q000378:metabolism; D020101:Acrosome Reaction / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004527:Egg Proteins / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015044:Zona Pellucida / Q000187:drug effects / Q000502:physiology*; D000072216:Zona Pellucida Glycoproteins
D000906:Antibodies; D004527:Egg Proteins; D004792:Enzyme Precursors; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D000072216:Zona Pellucida Glycoproteins; C022438:proacrosin; D000176:Acrosin