Detection of DNA using bioactive paper strips.
Paper strips containing DNA-conjugated microgels (MG) are used to achieve sensitive DNA detection in three steps: target DNA promoted ligation of a DNA primer to the MG-bound DNA, rolling circle amplification (RCA) between the primer and a circle DNA, and hybridization of the RCA products and a fluorescent DNA probe.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario, CanadaL8N 3Z5.
M Monsur
MM
Ali
Sergio D
SD
Aguirre
Yaqin
Y
Xu
Carlos D M
CD
Filipe
Robert
R
Pelton
Yingfu
Y
Li
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D010209:Paper
D004247:DNA