Peanut varieties with reduced Ara h 1 content indicating no reduced allergenicity.
Peanut allergy is a major cause of food-induced severe anaphylactic reactions. To date, no medical care is available to prevent and treat peanut allergy and therefore hypoallergenic peanut varieties are of considerable health political and economic interest. Major allergens that induce IgE-responses in peanut-sensitive patients are Ara h 1, Ara h 2 and Ara h 3/4. In order to identify hypoallergenic peanuts, commercially locally available peanut varieties were screened for their allergen content. Ara h 1-deficient peanuts from Southeast Asia were identified by SDS-PAGE, immunoblotting, inhibition assays and ELISA. 2-D PAGE analyses demonstrated the different compositions of the tested extracts and revealed a number of variations of the allergen patterns of peanuts from different varieties. Mediator release experiments of these peanut extracts demonstrated similar allergenicities as compared with standard peanut extract. These results indicate that the allergenicity of peanuts with reduced Ara h 1 content might be compensated by the other allergens, and thus do not necessarily cause a reduction of allergenicity.
Research Center Borstel, Molecular und Clinical Allergology, Borstel, Germany.
Susanne
S
Krause
Ties
T
Latendorf
Hendrik
H
Schmidt
Yasemin
Y
Darcan-Nicolaisen
Gerald
G
Reese
Arnd
A
Petersen
Ottmar
O
Janssen
Wolf-Meinhard
WM
Becker
D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D052179:Antigens, Plant / Q000032:analysis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D010367:Arachis / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology*; D001210:Asia, Southeastern; D015549:Basophil Degranulation Test; D001491:Basophils / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D004044:Dietary Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D004306:Dose-Response Relationship, Immunologic; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins; D009754:Nuts / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology*; D021183:Peanut Hypersensitivity / Q000276:immunology* / Q000503:physiopathology / Q000517:prevention & control; D010936:Plant Extracts / Q000032:analysis / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats; D013045:Species Specificity
D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D004044:Dietary Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; D010936:Plant Extracts; D010940:Plant Proteins