Inhibition of enzymatic degradation of adhesive-dentin interfaces.
Adhesive procedures activate dentin-associated matrix metalloproteinases (MMPs), and so iatrogenically initiate bond degradation. We hypothesized that adding MMP inhibitors to adhesive primers may prevent this endogenous enzymatic degradation, thereby improving bond durability. A non-specific MMP inhibitor (chlorhexidine) and a MMP-2/9-specific inhibitor (SB-3CT) were admixed to the primers of an etch & rinse and a self-etch adhesive, both considered as gold-standard adhesives within their respective categories. For dentin powder exposed to the adhesives under clinical application conditions, gelatin zymography revealed the release of MMP-2 (not of MMP-9) by the etch & rinse adhesive, while no release of enzymes could be detected for the mild self-etch adhesive, most likely because of its limited dentin demineralization effect. The built-in MMP inhibitors appeared effective in reducing bond degradation only for the etch & rinse adhesive, and not for the self-etch adhesive. Water sorption of adhesive interfaces most likely remains the principal mechanism of bond degradation, while endogenous enzymes appear to contribute to bond degradation of only etch & rinse adhesives.
Leuven BIOMAT Research Cluster, Department of Conservative Dentistry, School of Dentistry, Oral Pathology and Maxillo-facial Surgery, Catholic University of Leuven, Kapucijnenvoer 7, 3000 Leuven, Belgium. jan.demunck@med.kuleuven.be
J
J
De Munck
P E
PE
Van den Steen
A
A
Mine
K L
KL
Van Landuyt
A
A
Poitevin
G
G
Opdenakker
B
B
Van Meerbeek
D000042:Absorption; D000134:Acid Etching, Dental / Q000379:methods; D000327:Adsorption; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D001840:Dental Bonding; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2; D061965:Matrix Metalloproteinase Inhibitors; D046529:Microscopy, Electron, Transmission; D016877:Oxidants / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012973:Sodium Hypochlorite / Q000737:chemistry; D013314:Stress, Mechanical; D013450:Sulfones / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014867:Water / Q000737:chemistry; D055815:Young Adult
C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; D004791:Enzyme Inhibitors; D006573:Heterocyclic Compounds, 1-Ring; D061965:Matrix Metalloproteinase Inhibitors; C469607:OptiBond FL; D016877:Oxidants; D019279:Resin Cements; C429533:SB 3CT compound; D013450:Sulfones; D014867:Water; D012973:Sodium Hypochlorite; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D002710:Chlorhexidine