Development of cationic colloidal silica-coated magnetic nanospheres for highly selective and rapid enrichment of plasma membrane fractions for proteomics analysis.
PM (plasma membrane) proteins play critical roles in many biological processes and are often used as molecular targets for drug discovery. In PM proteome research, fast and highly selective methods for PM preparation are highly desirable for efficient PM protein identification. In the present study, an improved PM isolation technique involving coating intact cells with synthesized cationic silica-coated magnetic nanospheres was developed and applied to the proteomic analysis of the PM from human erythroleukaemia K562 cells. Western blotting characterization and protein identification of the prepared PM indicated that the PM enrichment method using the prepared magnetic nanospheres is a fast and inexpensive strategy with high specificity. Our results demonstrate the potential of these cationic silica-coated magnetic nanospheres for high-throughput identification of PM proteins from cells.
Li
Xuanwen
X
Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
Jia
Xueen
X
Xie
Chunliang
C
Lin
Yong
Y
Cao
Rui
R
He
Quanze
Q
Chen
Ping
P
Wang
Xianchun
X
Liang
Songping
S
D015153:Blotting, Western; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D003102:Colloids / Q000737:chemistry; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D008280:Magnetics; D008565:Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D040901:Proteomics / Q000191:economics / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*
D002412:Cations; D003102:Colloids; D008565:Membrane Proteins; D012822:Silicon Dioxide