Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp.
Thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as Klebsiella pneumoniae and Ralstonia sp. by 16S rDNA sequencing. The co-culture was able to degrade thiocyanate with degradation rate of 500 mg L(-1)d(-1) at 2,500 mg L(-1) thiocyanate concentration at pH 6.0 and 37 degrees C following thiocyanate hydrolase pathway. The Haldane kinetic model elucidates the growth and thiocyanate biodegradation kinetics of the co-culture with Ki value of 1,876 mg L(-1). The thiocyanate biodegradation kinetics was not affected by the additional supply of glucose. The very high activities of thiocyanate hydrolase, cyanide oxygenase, and cytochrome P-450 content during growth on thiocyanate were observed, showing the induction mechanism.
Biochemistry Division, Department of Chemistry, University of Pune, Pune, India.
Ashvini U
AU
Chaudhari
Kisan M
KM
Kodam
D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043367:Ralstonia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013696:Temperature; D013861:Thiocyanates / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D013861:Thiocyanates; D002244:Carbon; D009584:Nitrogen; C031760:thiocyanate