-509C>T polymorphism in the TGF-β1 gene promoter is not associated with susceptibility to and progression of colorectal cancer in Chinese.
OBJECTIVE
Colorectal cancer is common, accounting for nearly 10% of all cancers. Transforming growth factor-β1 (TGF-β1) is a pleiotropic cytokine that has been implicated in the pathogenesis of colorectal neoplasia. The most studied -509C>T polymorphism of TGF-β1 gene has been associated with various kinds of cancer. This study investigated the association between this genetic variant and the risk and/or progression of colorectal cancer.


METHODS
A case-control study was carried out of 150 colorectal cancer cases and 503 healthy controls. DNA was extracted from blood cell nuclear materials, and -509C>T polymorphism in the TGF-β1 gene promoter was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Colorectal cancer tissues (n = 70) were obtained from the studied cases for measurement of TGF-β1 mRNA expression levels. We also assessed the plasma TGF-β1 levels of cases (n = 88) and healthy subjects (n = 120).


RESULTS
The TGF-β1 producer genotype, -509TT, was not associated with an increased risk of colorectal cancer compared with other genotypes. Colorectal cancer patients especially those with a more aggressive disease behaviour were more frequently associated with C allele.


CONCLUSIONS
The results suggest that TGF-β1 -509C>T polymorphism is not associated with either an increased risk or progression of colorectal cancer.
Department of Laboratory Medicine, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.
P
P
Qi
C-P
CP
Ruan
H
H
Wang
F-G
FG
Zhou
Y-P
YP
Zhao
X
X
Gu
C-F
CF
Gao
D044466:Asian People; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D002903:Chromosomes, Human, 19-20 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D053773:Transforming Growth Factor beta1