Augmentation of radiation-induced apoptosis by ellagic acid.
This study evaluates the potential of ellagic acid (EA) as an enhancer of radiation-induced apoptosis in cancer cells. HeLa cells treated with EA and gamma radiation showed increased superoxide generation, upregulated p53 protein expression, and decreased antioxidant enzymes. We also found that EA and radiation enhance capase-3 activity via oxidative stress, increased intracellular calcium levels, and phospholipase C and cause a drop in mitochondrial potential. These results might provide a basis for prominent reduction of cancer cell using EA as an adjunct to radiotherapy and an opportunity to lower the toxic radiation doses to improve the quality of life.
Bhabha Atomic Research Centre, Radiation Biology and Health Sciences Division, Trombay, Mumbai, India.
Sushma Madhukar
SM
Bhosle
Vidhula R
VR
Ahire
Monica S
MS
Henry
Vikas S
VS
Thakur
Nagraj G
NG
Huilgol
Kaushala Prasad
KP
Mishra
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D053148:Caspase 3 / Q000378:metabolism; D004610:Ellagic Acid / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D018384:Oxidative Stress; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D013481:Superoxides; D004610:Ellagic Acid; D005979:Glutathione Peroxidase; D053148:Caspase 3