Biosensor for luteolin based on silver or gold nanoparticles in ionic liquid and laccase immobilized in chitosan modified with cyanuric chloride.
Novel and effective biosensors based on Ag or Au nanoparticles dispersed in ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate (BMI.PF(6)) and laccase (Lac) from Aspergillus oryzae immobilized in chitosan (Chi) chemically cross-linked with cyanuric chloride (CC) were constructed. This enzyme catalyzes the oxidation of luteolin to the corresponding o-quinone, which is electrochemically reduced back to luteolin at 0.35 V vs. Ag/AgCl. Square-wave voltammetry was used for the electrochemical determination of luteolin at the Lac-nanoparticles-BMI.PF(6) biosensors. The best performance was obtained with 50:20:15:15% (w/w/w/w) as the graphite powder:Chi-CC:Nujol:Ag-BMI.PF(6) or Au-BMI.PF(6) composition (Lac 0.29 units mL(-1)) in 0.1 M acetate buffer solution (pH 4.0) with frequency, pulse amplitude and scan increment at 50 Hz, 100 mV, and 5.0 mV, respectively. Under optimized conditions, the cathodic currents increased linearly for the luteolin concentration range of 0.099-5.825 microM with detection limits of 0.054 +/- 0.004 microM (Ag-BMI.PF(6)) and 0.028 +/- 0.002 microM (Au-BMI.PF(6)). These biosensors demonstrated high sensitivity, good repeatability and reproducibility, and long-term stability (13% decrease in response over 70 days). The recovery study for luteolin in chamomile tea samples gave values of 91.8-104.8%. The influence of Lac immobilized in Chi-CC and nanoparticles-BMI.PF(6) contributes to the excellent performance of the biosensors.
Departamento de Química, Laboratório de Biossensores, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, Santa Catarina, Brazil. aninhafranzoi@yahoo.com.br
Ana Cristina
AC
Franzoi
Iolanda Cruz
IC
Vieira
Jairton
J
Dupont
Carla Weber
CW
Scheeren
Luciane França
LF
de Oliveira
D001236:Aspergillus oryzae / Q000201:enzymology; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D020843:Chamomile / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D047311:Luteolin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry; D014227:Triazines / Q000737:chemistry*
C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D007093:Imidazoles; D052578:Ionic Liquids; D014227:Triazines; D012834:Silver; C019309:cyanuric chloride; D006046:Gold; D048271:Chitosan; D042845:Laccase; D047311:Luteolin