Deoxynivalenol and nivalenol inhibit lipopolysaccharide-induced nitric oxide production by mouse macrophage cells.
Deoxynivalenol (DON) and nivalenol (NIV), trichothecene mycotoxins, are secondary metabolites produced by Fusarium fungi. Trichothecene mycotoxins cause immune dysfunction, thus leading to diverse responses to infection. The present study evaluated the effect of DON and NIV on nitric oxide (NO) production by RAW264 cells stimulated with lipopolysaccharide (LPS). LPS-induced NO production was reduced in the presence of these toxins. The transcriptional activation and expression of inducible NO synthase (iNOS) by LPS were also repressed by these toxins. DON or NIV inhibited LPS-induced expression of interferon-beta (IFN-beta), which plays an indispensable role in LPS-induced iNOS expression. These results indicate that DON and NIV inhibit the LPS-induced NO and IFN-beta production, which both play an important role for host protection against invading pathogens, and suggests that the inhibition of these factors may be involved in the immunotoxic effects of these mycotoxins.
Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan. sugiyama@nihs.go.jp
Kei-ichi
K
Sugiyama
Masashi
M
Muroi
Ken-ichi
K
Tanamoto
Motohiro
M
Nishijima
Yoshiko
Y
Sugita-Konishi
D000818:Animals; D002460:Cell Line; D005670:Fusarium / Q000472:pathogenicity; D016899:Interferon-beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009183:Mycotoxins / Q000633:toxicity*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*
D008070:Lipopolysaccharides; D009183:Mycotoxins; D014255:Trichothecenes; D009569:Nitric Oxide; C038405:nivalenol; D016899:Interferon-beta; D019001:Nitric Oxide Synthase; C007262:deoxynivalenol