An exploratory NMR nutri-metabonomic investigation reveals dimethyl sulfone as a dietary biomarker for onion intake.
The metabolome following intake of onion by-products is evaluated. Thirty-two rats were fed a diet containing an onion by-product or one of the two derived onion by-product fractions: an ethanol extract and the residue. A 24 hour urine sample was analyzed using (1)H NMR spectroscopy in order to investigate the effects of onion intake on the rat metabolism. Application of interval extended canonical variates analysis (ECVA) proved to be able to distinguish between the metabolomic profiles from rats consuming normal feed and rats fed with an onion diet. Two dietary biomarkers for onion intake were identified as dimethyl sulfone and 3-hydroxyphenylacetic acid. The same two dietary biomarkers were subsequently revealed by interval partial least squares regression (PLS) to be perfect quantitative markers for onion intake. The best PLS calibration model yielded a root mean square error of cross-validation (RMSECV) of 0.97% (w/w) with only 1 latent variable and a squared correlation coefficient of 0.94. This indicates that urine from rats on the by-product diet, the extract diet, and the residue diet all contain the same dietary biomarkers and it is concluded that dimethyl sulfone and 3-hydroxyphenylacetic acid are dietary biomarkers for onion intake. Being able to detect specific dietary biomarkers is highly beneficial in the control of nutritionally enhanced functional foods.
University of Copenhagen, Faculty of Life Sciences, Dept. of Food Science, Quality & Technology, Rolighedsvej 30, 1958 Frederiksberg C, Denmark.
Hanne
H
Winning
Eduvigis
E
Roldán-Marín
Lars O
LO
Dragsted
Nanna
N
Viereck
Morten
M
Poulsen
Concepción
C
Sánchez-Moreno
M Pilar
MP
Cano
Søren B
SB
Engelsen
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D004032:Diet; D004121:Dimethyl Sulfoxide / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D004435:Eating; D000431:Ethanol / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D009747:Nutritional Physiological Phenomena; D019697:Onions / Q000737:chemistry; D010648:Phenylacetates / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D025341:Principal Component Analysis; D051381:Rats; D012995:Solubility; D013450:Sulfones / Q000737:chemistry / Q000378:metabolism* / Q000652:urine
D000975:Antioxidants; D015415:Biomarkers; D010648:Phenylacetates; D010936:Plant Extracts; D013450:Sulfones; D000431:Ethanol; C025910:dimethyl sulfone; C008069:3-hydroxybenzeneacetic acid; D004121:Dimethyl Sulfoxide