Two-way communication between endometrial stromal cells and monocytes.
Immune system cells and cells of the endometrium have long been proposed to interact in both physiological and pathological processes. The current study was undertaken to examine communication between cultured monocytes and endometrial stromal cells and also to assess responses of endometrial stromal cells for treatment with estradiol (E) in the absence and presence of medroxyprogesterone acetate (P). A telomerase-immortalized human endometrial stromal cell (T-HESC) line and the U937 monocyte cell line were used. Telomerase-immortalized human endometrial stromal cells were treated with E +/- P +/- monocyte conditioned medium; U937 were treated +/- T-HESC conditioned medium. Gene expression in response to treatment was examined by DNA microarray. Bidirectional communication, as demonstrated by changes in gene expression, clearly occurred between U937 monocytes and T-HESC.
Klinkova
Olga
O
Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, South Dakota 57069, USA.
Hansen
Keith A
KA
Winterton
Emily
E
Mark
Connie J
CJ
Eyster
Kathleen M
KM
D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002461:Cell Line, Transformed; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004715:Endometriosis / Q000473:pathology* / Q000503:physiopathology; D004717:Endometrium / Q000166:cytology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D009000:Monocytes / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D011388:Prolactin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology*; D019098:Telomerase / Q000235:genetics; D020298:U937 Cells
D017077:Culture Media, Conditioned; D004958:Estradiol; D011388:Prolactin; D019098:Telomerase