Changes in cerebral arterial, tissue and venous oxygenation with evoked neural stimulation: implications for hemoglobin-based functional neuroimaging.
Little is known regarding the changes in blood oxygen tension (P(O2)) with changes in brain function. This work aimed to measure the blood P(O2) in surface arteries and veins as well as tissue with evoked somato-sensory stimulation in the anesthetized rat. Electrical stimulation of the forepaw induced average increases in blood flow of 44% as well as increases in the tissue P(O2) of 28%. More importantly, increases in P(O2) throughout pial arteries (resting diameters=59 to 129 microm) and pial veins (resting diameters=62 to 361 microm) were observed. The largest increases in vascular P(O2) were observed in the small veins (from 33 to 40 mm Hg) and small arteries (from 78 to 88 mm Hg). The changes in oxygen saturation (S(O2)) were calculated and the largest increases were observed in small veins (Delta=+11%) while its increase in small arteries was small (Delta=+4%). The average diameter of arterial vessels was observed to increase by 4 to 6% while that of veins was not observed to change with evoked stimulation. These findings show that the increases in arterial P(O2) contribute to the hyper-oxygenation of tissue and, mostly likely, also to the signal changes in hemoglobin-based functional imaging methods (e.g. BOLD fMRI).
Vazquez
Alberto L
AL
Department of Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15203, USA.
Fukuda
Mitsuhiro
M
Tasker
Michelle L
ML
Masamoto
Kazuto
K
Kim
Seong-Gi
SG
D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003952:Diagnostic Imaging / Q000379:methods*; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D006454:Hemoglobins / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D006454:Hemoglobins