Role of IL-1beta on the glutamine synthetase in retinal Müller cells under high glucose conditions.
OBJECTIVE
To investigate (1) the role of cytokine interleukin-1beta on the glutamine synthetase in retinal Müller cells under high glucose condition, (2) the mechanism for down-regulation of glutamine synthetase in retinal Müller cells induced by interleukin-1beta under high glucose conditions.


METHODS
The effect of interleukin-1beta on the expression of glutamine synthetase and c-Jun in retinal Müller cells under normal and high glucose conditions was measured by immunocytochemistry, Western blot, and real-time (RT) PCR, and was further confirmed by c-Jun siRNA method.


RESULTS
Under high glucose conditions, interleukin-1beta significantly increased expression of c-Jun and decreased the expression of glutamine synthetase. When c-Jun gene was silenced by siRNA, interleukin-1beta could not decrease glutamine synthetase expression in high glucose concentrations.


CONCLUSIONS
Interleukin-1beta decreases expression of glutamine synthetase via activation of c-Jun. The data suggested that interleukin-1beta may play a role in the development of diabetic retinopathy.
Shen
Xi
X
Department of Ophthalmology, Ruijin Hospital, School of Medicine, The Jiaotong University, Shanghai, China.
Xu
Gezhi
G
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002478:Cells, Cultured; D015536:Down-Regulation; D019084:Fluorescent Antibody Technique, Indirect; D020868:Gene Silencing; D005947:Glucose / Q000494:pharmacology*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000502:physiology*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000502:physiology; D051381:Rats; D055351:Retinal Neurons / Q000187:drug effects* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053583:Interleukin-1beta; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D005974:Glutamate-Ammonia Ligase; D005947:Glucose