Haloarchaeal diversity in 23, 121 and 419 MYA salts.
DNA was extracted from surface-sterilized salt of different geological ages (23, 121, 419 million years of age, MYA) to investigate haloarchaeal diversity. Only Haloarcula and Halorubrum DNA was found in 23 MYA salt. Older crystals contained unclassified groups and Halobacterium. The older crystals yielded a unique 55-bp insert within the 16S rRNA V2 region. The secondary structure of the V2 region completely differed from that in haloarchaea of modern environments. The DNA demonstrates that unknown haloarchaea and the Halobacterium were key components in ancient hypersaline environments. Halorubrum and Haloarcula appear to be a dominant group in relatively modern hypersaline habitats.
Canadian Institute for Advanced Research, Program in Integrated Microbial Diversity, and Department of Biology, Dalhousie University, Halifax, Canada.
J S
JS
Park
R H
RH
Vreeland
B C
BC
Cho
T K
TK
Lowenstein
M N
MN
Timofeeff
W D
WD
Rosenzweig
D001483:Base Sequence; D044822:Biodiversity; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D019613:Haloarcula / Q000235:genetics* / Q000302:isolation & purification; D006217:Halobacterium / Q000235:genetics* / Q000302:isolation & purification; D055352:Halorubrum / Q000235:genetics* / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012492:Salts