A novel assay for influenza virus quantification using surface plasmon resonance.
Quantification of hemagglutinin (HA) by single-radial immuno diffusion (SRID) is the predominant method to ensure product potency in seasonal influenza vaccines. Here a new method for quantification of influenza virus using biosensor technology is presented. The method employs quantification of virus via an inhibition assay format using HA proteins for H1N1, H3N2 and B immobilized on a sensor chip. Initial results showed the assay to have higher sensitivity (detection range 0.5-10 microg/ml), higher precision and significantly lower analysis and hands on time compared with SRID.
Nilsson
C Estmer
CE
GE Healthcare BioSciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden.
Abbas
S
S
Bennemo
M
M
Larsson
A
A
Hämäläinen
M D
MD
Frostell-Karlsson
A
A
D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007252:Influenza Vaccines; D011786:Quality Control; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors; D019562:Viral Load; D014773:Virology / Q000379:methods*
D007252:Influenza Vaccines