Effects of chlorophyllin on replication of poliovirus and bovine herpesvirus in vitro.
OBJECTIVE
Chlorophyllin (CHLN), a synthetic derivative of chlorophyll, was assayed in the replication of poliovirus (PV-1) and bovine herpesvirus (BoHV-1) in HEp-2 cell cultures.


RESULTS
Virucidal activity of CHLN was evaluated and the time-of-addition assay was performed as follows: before the infection (-1 and -2 h), at the time of the infection (0 h) and after the infection (1 and 2 h). Plaque reduction assay (PRA) showed that CHLN inhibited BoHV-1 and PV-1 infection and the 50% inhibitory concentrations (IC(50)) against BoHV-1 and PV-1 infection were 8.6 and 19.8 microg ml(-1), respectively. The time-of-addition study demonstrated that the CHLN was effective inhibiting viral replication in 51% and 66.5% for PV-1 and BoHV-1, respectively, at the highest concentration of 20.0 microg ml(-1), when added during the infection. The directed effect of CHLN on viral strains demonstrated an inhibition of 62% and 66.4% for PV-1 and BoHV-1, respectively, by PRA.


CONCLUSIONS
These results demonstrated that CHLN could be used as an antiviral suggesting directed activity on virus particles and on virus-receptor sites to BoHV. For poliovirus, CHLN also demonstrated virucide activity, moreover, showed to inhibit early steps of the replication cycle.


CONCLUSIONS
CHLN demonstrated promising selectivity index for both virus strains; therefore, it can be used for the development of an antiviral agent.
Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, CEP, Brasil.
F J
FJ
Benati
F
F
Lauretti
L C
LC
Faccin
B
B
Nodari
D V
DV
Ferri
M S
MS
Mantovani
R E C
RE
Linhares
C
C
Nozawa
D000998:Antiviral Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002735:Chlorophyllides / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006564:Herpesviridae / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D020128:Inhibitory Concentration 50; D017955:Poliovirus / Q000187:drug effects / Q000502:physiology*; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D002735:Chlorophyllides; C007020:chlorophyllin