Rhodobacter johrii sp. nov., an endospore-producing cryptic species isolated from semi-arid tropical soils.
An oval to rod-shaped, phototrophic, purple non-sulfur bacterium, strain JA192(T), was isolated from an enrichment culture of a pasteurized rhizosphere soil sample from a field cultivated with jowar (sorghum) collected from Godumakunta village near Hyderabad, India. Strain JA192(T) is Gram-negative, motile and produces endospores. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that the strain JA192(T) is closely related to Rhodobacter sphaeroides 2.4.1(T) (99.9 % sequence similarity), Rba. megalophilus JA194(T) (99.8 %) and Rba. azotoformans KA25(T) (98.1 %) and clusters with other species of the genus Rhodobacter of the family Rhodobacteraceae. However, DNA-DNA hybridization with Rba. sphaeroides DSM 158(T), Rba. megalophilus JA194(T) and Rba. azotoformans JCM 9340(T) showed relatedness of only 38-57 % with respect to strain JA192(T). On the basis of 16S rRNA gene sequence analysis, DNA-DNA hybridization data and morphological, physiological and chemotaxonomic characters, strain JA192(T) represents a novel species of the genus Rhodobacter, for which the name Rhodobacter johrii sp. nov. is proposed. The type strain is JA192(T) (=DSM 18678(T) =JCM 14543(T) =MTCC 8172(T)).
Bacterial Discovery Laboratory and Resource Centre, Centre for Environment, IST, JNT University, Kukatpally, Hyderabad 500 085, India.
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Girija
Bacterial Discovery Laboratory and Resource Centre, Centre for Environment, IST, JNT University, Kukatpally, Hyderabad 500 085, India.
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Sasikala
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India.
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Ramana
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
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Spröer
Department of Biology, Nippon Medical School, Kosugi-cho, Nakahara, Kawasaki 211-0063, Japan.
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Takaichi
Leibniz-Institut für Meereswissenschaften IFM-GEOMAR, Marine Mikrobiologie, Düsternbrooker Weg 20, 24105 Kiel, Germany.
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Thiel
Leibniz-Institut für Meereswissenschaften IFM-GEOMAR, Marine Mikrobiologie, Düsternbrooker Weg 20, 24105 Kiel, Germany.
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Imhoff
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016992:Rhodobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012988:Soil Microbiology; D045868:Sorghum / Q000382:microbiology; D013171:Spores, Bacterial / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S