Distribution of G (VP7) and P (VP4) genotypes of group A bovine rotaviruses from Turkish calves with diarrhea, 1997-2008.
Group A rotaviruses are major enteric pathogens of calves. In order to investigate the genetic diversity of bovine rotaviruses (BRVs), a collection of 53 BRVs, detected from diarrheic calves from several Turkish geographical areas, between 1997 and 2008 was analyzed by RT-PCR for specificities of the outer capsid proteins VP7 (G type) and VP4 (P type), for the first time. Overall, G6 was the predominant G type, detected in 40/53 samples (75.4%), while P[11] was the predominant P type, detected in 52/53 samples (98.1%). The most common VP7/VP4 combinations were G6P[11] (60.3%) and G10P[11] (24.5%). Mixed infections were identified in 7/53 samples (13.2%). In the VP7 region the G6P[11] viruses were similar to other ones detected worldwide, forming an independent G6 lineage, distantly related to the G6 gene of the vaccine G6P[1] strain NCDV (90.1% amino acid identity), and suggesting that G6P[11] viruses represent a genetically stable BRV strain. The study of G and P type diversity is pivotal to understand the efficacy of the existing rotavirus vaccines and to provide the basis of future prophylaxis tools against rotaviral diarrhea of calves.
Alkan
Feray
F
Ankara University, Faculty of Veterinary Medicine, Irfan Bastug Cad, Diskapi, 06110 Ankara, Turkey. falkan@veterinary.ankara.edu.tr
Ozkul
Aykut
A
Oguzoglu
Tuba C
TC
Timurkan
Mehmet O
MO
Caliskan
Elvin
E
Martella
Vito
V
Burgu
Ibrahim
I
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000209:etiology*; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D014421:Turkey
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus