Eudragit S100 microparticles containing Spodoptera frugiperda nucleopolyehedrovirus: physicochemical characterization, photostability and in vitro virus release.
The aim of this study was to encapsulate the occlusion bodies (OBs) of Spodoptera frugiperda nucleopolyhedrovirus (SfNPV) in Eudragit S100 microparticles (MPs), considering this technique as a possible alternative to protect them from deleterious environmental conditions. The MPs were prepared by oil-in-oil emulsion (O/O) solvent evaporation method. Experimental conditions were established according to a previous multi-level experimental design involving the core/polymer ratio as independent variable. The effects of these parameters on particle size and process yield were investigated observing that polymer concentration had a significant effect on particle size. After adequate conditions for MPs formation were determined, virus was encapsulated. The virus microparticles presented a particle size between 50-300 microm and concentration was 2.62 x 10(9) OBs g(-1). Microencapsulation efficiency was 53.43% and virus release adjusted to Higuchi model suggesting diffusion as the release mechanism. Evaluated microencapsulation process protected viral particles of UV-inactivation, suggesting its potential for a biopesticide development.
Centro de Biotecnología y Bioindustria, Corpoica, Km 14 Vía Mosquera, Bogotá, Colombia. laurafernandav@yahoo.es
Laura
L
Villamizar
Gloria
G
Barrera
Alba Marina
AM
Cotes
Fleming
F
Martínez
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002627:Chemistry, Physical; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D008027:Light; D008855:Microscopy, Electron, Scanning; D017924:Nucleopolyhedroviruses / Q000737:chemistry*; D018411:Spodoptera; D014764:Viral Proteins / Q000737:chemistry*; D057074:Virus Release / Q000528:radiation effects
D000180:Acrylic Resins; D014764:Viral Proteins; C050528:Eudragit RS