[Promotive effect of LRP16 gene on proliferation of K562 cells].
The study was aimed to investigate the promotive effect of LRP16 gene on K562 cell proliferation. Open reading frame of LRP16 gene was amplified using reverse transcription-polymerase chain reaction (RT-PCR) and ligated to pGEM-T plasmid to construct LRP16 ORF-pGEM-T recombinant vector. Then, LRP16 ORF identified by sequencing was inserted into pcDNA3.1+ plasmid to construct LRP16 ORF-pcDNA3.1+ recombinant expression plasmid which was transfected into K562 cell lines to make overexpression of LRP16 gene in K562 cells. Survival of cells was determined by MTT assay and growth curve of cells was drawn, the cell cycle was detected by flow cytometry. The results showed that LRP16 ORF was successfully amplified, then the LRP16 ORF-pcDNA3.1+ recombinant plasmid was constructed. The K562 cell line with overexpression of LRP16 gene was established. The promotive effect of LRP16 gene overexpression on proliferation of K562 cells was observed and the effect partially related to the enhancement of cells from G0 to S phase induced by LRP16 gene. It is concluded that LRP16 gene overexpression shows a promotive effect on proliferation of K562 cells.
Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Bo
B
Yang
Xue-Chun
XC
Lu
Xiao-Hua
XH
Chi
Wei-Dong
WD
Han
Li
L
Yu
Fang-Ding
FD
Lou
D002265:Carboxylic Ester Hydrolases; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D009363:Neoplasm Proteins / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids
D009363:Neoplasm Proteins; D002265:Carboxylic Ester Hydrolases; C476832:MACROD1 protein, human