In vitro small intestinal epithelial cell growth on a nanocomposite polycaprolactone scaffold.
Tissue engineering of the small intestine remains experimental despite worldwide attempts to develop a functional substitute for short bowel syndrome. Most published studies have reported predominant use of PLLA (poly-L-lactide acid)/PGA (polyglycolic acid) copolymer as the scaffold material, and studies have been limited by in vivo experiments. This lack of progress has inspired a fresh perspective and provoked further investigation and development in this field of tissue engineering. In the present paper, we exploit a relatively new nanocomposite of POSS (polyhedral oligomeric silsesquioxane) and PCL [poly(caprolactone-urea)urethane] as a material to develop porous scaffolds using a solvent casting/particulate leaching technique to fabricate porous scaffolds in different pore sizes and porosities. Scaffolds were characterized for pore morphology and porosity using scanning electron microscopy and micro-computed tomography. Rat intestinal epithelial cells were then seeded on to the polymer scaffolds for an in vitro study of cell compatibility and proliferation, which was assessed by Alamar Blue and lactate dehydrogenase assays performed for 21 days post-seeding. The results obtained demonstrate that POSS-PCL nanocomposite was produced as a macroporous scaffold with porosity over the range of 40-80% and pore size over the range of 150-250 microm. This scaffold was shown to support epithelial cell proliferation and growth. In conclusion, as a further step in investigating small intestinal tissue engineering, the nanocomposite employed in this study may prove to be a useful alternative to poly(lactic-co-glycolic acid) in the future.
Gupta
Ashish
A
Centre for Nanotechnology, Biomaterials and Tissue Engineering, University College London, London, U.K.
Vara
Dina S
DS
Punshon
Geoffrey
G
Sales
Kevin M
KM
Winslet
Marc C
MC
Seifalian
Alexander M
AM
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology* / Q000201:enzymology; D007421:Intestine, Small / Q000166:cytology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D017646:Organosilicon Compounds; D011091:Polyesters; C510837:trisilanolisobutyl-POSS; C016240:polycaprolactone; D007770:L-Lactate Dehydrogenase