NO-loaded Zn(2+)-exchanged zeolite materials: a potential bifunctional anti-bacterial strategy.
Nitric oxide (NO) is important for the regulation of a number of diverse biological processes, including vascular tone, neurotransmission, inflammatory cell responsiveness, defence against invading pathogens and wound healing. Transition metal exchanged zeolites are nanoporous materials with high-capacity storage properties for gases such as NO. The NO stores are liberated upon contact with aqueous environments, thereby making them ideal candidates for use in biological and clinical settings. Here, we demonstrate the NO release capacity and powerful bactericidal properties of a novel NO-storing Zn(2+)-exchanged zeolite material at a 50 wt.% composition in a polytetrafluoroethylene polymer. Further to our published data showing the anti-thrombotic effects of a similar NO-loaded zeolite, this study demonstrates the anti-bacterial properties of NO-releasing zeolites against clinically relevant strains of bacteria, namely Gram-negative Pseudomonas aeruginosa and Gram-positive methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Clostridium difficile. Thus our study highlights the potential of NO-loaded zeolites as biocompatible medical device coatings with anti-infective properties.
Fox
Sarah
S
University of Edinburgh, MRC Centre for Inflammation Research, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. sfox1@staffmail.ed.ac.uk
Wilkinson
Tom S
TS
Wheatley
Paul S
PS
Xiao
Bo
B
Morris
Russell E
RE
Sutherland
Alistair
A
Simpson
A John
AJ
Barlow
Peter G
PG
Butler
Anthony R
AR
Megson
Ian L
IL
Rossi
Adriano G
AG
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009569:Nitric Oxide / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors; D017641:Zeolites / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D017641:Zeolites; D009569:Nitric Oxide; D015032:Zinc