Inverse-fluorescence correlation spectroscopy.
An alternative version of fluorescence correlation spectroscopy is presented, where the signal from a medium surrounding the particles of interest is analyzed, as opposed to a signal from the particles themselves. This allows for analysis of unlabeled particles and potentially of biomolecules. Here, the concept together with principal experiments on polystyrene beads of 100, 200, 400, and 800 nm diameter in an aqueous solution of alexa 488-fluorophores are presented. The use of photo detectors allowing higher photon fluxes, or of reduced detection volumes, should enable analysis of significantly smaller particles or even biomolecules.
Department of Applied Physics, Experimental Biomolecular Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden. stewen@kth.se
Stefan
S
Wennmalm
Per
P
Thyberg
Lei
L
Xu
Jerker
J
Widengren
D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D011137:Polystyrenes