Prostasin inhibits cell invasion in human choriocarcinomal JEG-3 cells.
Controlled invasion of the uterine wall by the trophoblast cells is pivotal for the successful pregnancy, and various kinds of protease are involved in this process. Serine protease prostasin has been shown to participate in the proteolytic activation of epithelial sodium channel as well as cleavage of epidermal growth factor receptor extracellular domain in human epithelial cells. Its physiological significance in human placentation has been suggested but not validated. In the present study, we found that prostasin was expressed at a relatively high level in human placenta trophoblasts in early pregnant weeks. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. Our data indicated that this serine protease may function as an invasion suppressor in human trophoblast, participating in the invasion-restrictive regulation of trophoblasts to avoid their over-penetration into the uterine wall.
Ma
Xiao-jie
XJ
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West Road, ChaoYang District, 100101, Beijing, China.
Fu
Ya-yuan
YY
Li
Yu-xia
YX
Chen
Li-mei
LM
Chai
Karl
K
Wang
Yan-ling
YL
D045744:Cell Line, Tumor; D002465:Cell Movement; D002822:Choriocarcinoma / Q000473:pathology*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D053505:Matrix Metalloproteinases, Secreted / Q000096:biosynthesis; D009361:Neoplasm Invasiveness; D011247:Pregnancy; D012697:Serine Endopeptidases / Q000502:physiology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis; D014327:Trophoblasts / Q000166:cytology; D014599:Uterus / Q000166:cytology; D000097792:Tissue Inhibitor of Metalloproteinase-4; D000099271:Prostasin
D020778:Matrix Metalloproteinase 2; D053505:Matrix Metalloproteinases, Secreted; D012697:Serine Endopeptidases; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D000097792:Tissue Inhibitor of Metalloproteinase-4; D000099271:Prostasin; C410599:MMP26 protein, human