Sterility testing by CE: a comparison of online preconcentration approaches in capillaries with greater internal diameters.
Detection of microbial contamination is of critical importance in the medical and the food industry. Rapid tests for the absence or presence of viable microorganisms are in urgent demand. CE is a modern analytical technique that can be adapted for rapid screening of microbial contamination. However, the small dimensions of capillaries allow the introduction of only a small fraction of the sample, which can be problematic when examining large samples. In this article, we examine the possibilities of introducing larger sample volumes using capillaries with greater id together with different stacking techniques. The use of 0.32 mm id capillary and the injection of 60% of the capillary volume led to approximately 120-fold improvement of the injected sample volume over the classical injection 5% of a 0.10-mm id capillary. The setup we described opens new possibilities in sterility testing using CE.
Petr
Jan
J
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA.
Jiang
Chunxia
C
Sevcik
Juraj
J
Tesarova
Eva
E
Armstrong
Daniel W
DW
D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation; D012475:Salmonella / Q000302:isolation & purification