Purification of intact microtubules from brain.
Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.
Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903.
J B
JB
Kirkpatrick
L
L
Hyams
V L
VL
Thomas
P M
PM
Howley