Effects of pomegranate juice on hyperoxaluria-induced oxidative stress in the rat kidneys.
To evaluate the role of the inducible nitric oxide synthase (iNOS), selective nuclear factor-kB (NF-kB), and p38-mitogene-activated protein kinase (p38-MAPK) on hyperoxaluria-induced oxidative stress and stone formation in rat kidneys. The rats were divided into five groups: group 1, control group; group 2: ethylene glycol (EG) group; group 3: EG + pomegranate juice (PJ)-low group; group 4: EG + PJ-middle group; group 5: EG + PJ-high group. Rats were sacrificed on 7, 15, and 45 days. The iNOS expression, p65-NF-kB and p38-MAPK activity, and oxidative stress markers were evaluated in the kidney. Crystal depositions were evident on day 7, and mild and severe crystallization were observed on day 15 and 45 in EG group, respectively. There was limited or no crystal formation in rats in both middle- and high-dose PJ groups when compared to low-dose PJ group. Crystal depositions, iNOS, p38-MAPK and p65-NF-kB activity, and oxidative stress markers were found to be decreased by middle- and high-dose PJ treatment. PJ was found to have inhibitory effects on renal tubular cell injury and oxidative stress caused by oxalate crystals by reducing ROS, iNOS, p38-MAPK, and NF-kB expression.
Department of Urology, Bezm-i Alem Valide Sultan Vakif Gureba Research and Education Hospital, Istanbul, Turkey. ozlemyusufilbey@hotmail.com
Yusuf Ozlem
YO
Ilbey
Emin
E
Ozbek
Abdulmuttalip
A
Simsek
Mustafa
M
Cekmen
Adnan
A
Somay
Ali Ihsan
AI
Tasci
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001806:Blood Urea Nitrogen; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D006959:Hyperoxaluria / Q000378:metabolism*; D007150:Immunohistochemistry; D007669:Kidney Calculi / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D029561:Lythraceae; D008297:Male; D008854:Microscopy, Electron; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods*; D028321:Plant Preparations / Q000494:pharmacology; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016482:Urinalysis
D015415:Biomarkers; D016328:NF-kappa B; D028321:Plant Preparations; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D005978:Glutathione