Dual effects of hyperbaric oxygen on proliferation and cytotoxic T lymphocyte activity of rat splenic lymphocytes.
Contradictory reports exist regarding the effects of hyperbaric oxygen (HBO2) on immune functions. We hypothesized that the intensity of exposure is a key factor, which leads to the different effects of HBO2 on immunity. In this study, we determined the function of rat splenic lymphocytes after in vitro exposure to different pressure-durations of HBO2. The proliferation rate and total cytotoxic T lymphocyte (CTL) activity stimulated by concanavalin A were enhanced by exposure to low (100 kPa for 60 or 90 minutes and 150 kPa for 30 or 60 minutes) HBO2 pressure-durations, but were inhibited by exposure to higher pressure-durations. We conclude that HBO2 exerts dual effects on lymphocyte function, as appropriate exposure promotes and excessive exposure inhibits the functions.
Liu
W
W
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, PR China.
Zhang
J
J
Ma
C
C
Liu
Y
Y
Li
R
R
Sun
X
X
Zhang
J
J
Xu
W G
WG
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D006931:Hyperbaric Oxygenation; D007111:Immunity, Cellular / Q000187:drug effects; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D010100:Oxygen