[Screening the differential expression of adriamycin-resistance related genes of breast cancer by cDNA microarray].
OBJECTIVE
To study the differential gene expression of adriamycin-resistance of breast cancer cell line MCF-7/ADR as compared with its parent cell MCF-7 and screen the related genes.


METHODS
The in vitro inhibitory rate of MCF-7/ADR and MCF-7 cell lines to ADM (adriamycin) was examined by MIT assay. Then a cDNA microarray representing 14,755 genes was used to analyze the expression profiles of these two breast cancer cell lines.


RESULTS
The inhibitory rate of MCF-7/ ADR to ADM was lower than that of MCF-7(P <0.05)and the drug resistance to ADR of MCF-7/ADR was stronger than that of MCF-7. There were 2374 differential expression genes in MCF-7/adr and MCF-7 cell lines, of which 1099 genes upregulated with 99 genes 10-fold upregulated and 1275 genes downregulated with 71 genes 10-fold downregulated. The important upregulated genes were Bcl-2, GSTP1, c-myc, MMP-1 and NNMT while those downregulated ones p53, p21, p27 and CYPIA1.


CONCLUSIONS
Drug resistance of breast cancer is a complicated process involving the expression of many upregulated or downregulated genes. The application of gene chip in screening drug-resistant genes may be a new approach for selecting the optimal chemotherapeutic regimens. [Key words]
Department of General Surgery Oncology, General Coal Hospital, Beijing 100028, China. penghao009@sina.com
Hao
H
Peng
Hua-Wei
HW
Yang
Li-Wei
LW
Song
Zheng
Z
Zhou
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin