[Preliminary studies on the function of Arabidopsis CK1A gene].
A casein kinase 1 protein gene, CK1A, was isolated from Arabidopsis seedlings by RT-PCR method. This gene contains an open reading frame of 2,112 bp, which encodes 703 amino acids. The plant expression vector of 35S: GFP: CK1A was constructed by the Gateway System. The 35S: GFP: CK1A fusion protein was localized to the nucleus in onion epidermal cell, indicating that the product of CK1A gene plays a role in the cell nucleus. The semi-quantitative RT-PCR analysis showed that CK1A was highly expressed in flowers, stems and roots, but less in leaves and leafstalks. The yeast two-hybrid analysis demonstrated that CK1A and CRY2 can interact in vivo under blue light, which indicates that CK1A may play an important role in blue light signal induction of Arabidopsis.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Life Science and Technology, Hunan University, Changsha 410082, China.
Da-Shi
DS
Yu
Qiong
Q
Zhao
Ke-Qin
KQ
Deng
Xin-Hong
XH
Guo
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D047389:Casein Kinase I / Q000378:metabolism*; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; C103618:CRY2 protein, Arabidopsis; D056931:Cryptochromes; D047389:Casein Kinase I