FT-Raman and energy dispersive X-ray fluorescence spectrometric analyses of enamel submitted to 38% hydrogen peroxide bleaching, an acidic beverage, and simulated brushing.
OBJECTIVE
This study aimed to investigate the effects on enamel surface treated with hydrogen peroxide bleaching and acidic soft drink immersion and/or brushing with whitening dentifrices.


METHODS
Fifty-six standardized enamel slabs obtained from labial surfaces of bovine incisors were used. Enamel slabs were ground flat, polished, and randomly assigned to one of seven treatment groups: (1) control, in which no treatment was performed, (2) soft drink immersion, (3) 38% hydrogen peroxide bleaching, (4) simulated toothbrushing with whitening dentifrice, (5) soft drink immersion and bleaching, (6) soft drink immersion, bleaching, and toothbrushing, and (7) bleaching and toothbrushing. The mineral concentration of enamel surfaces was determined before and after treatments by means of Fourier transform (FT)-Raman spectroscopy and energy dispersive x-ray fluorescence spectrometry (EDXRF). Data were statistically analyzed by ANOVA and Tukey test (p < 0.05).


RESULTS
Raman spectroscopy results indicated that enamel mineral content decreased after all treatments except in group 1, whereas EDXRF results exhibited mineral decrease in groups 3, 4, 5, and 7.


CONCLUSIONS
Bleaching alone or in combination with soft drink immersion and brushing decreases enamel mineral content.
Department of Operative Dentistry, University of Taubaté, Taubaté, São Paulo, Brazil.
Shirley de Souza
Sde S
Paula
Luís Eduardo Silva
LE
Soares
Ana Maria
AM
do Espírito Santo
Airton Abrahão
AA
Martin
Vanessa
V
Cavalli
Priscila Christiane Suzy
PC
Liporoni
D002253:Carbonated Beverages; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects*; D003802:Dentifrices / Q000627:therapeutic use; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000494:pharmacology*; D008422:Materials Testing; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D014073:Tooth Bleaching / Q000379:methods; D014099:Toothbrushing
D003802:Dentifrices; D006861:Hydrogen Peroxide