Mercury(II) recognition and fluorescence imaging in vitro through a 3D-complexation structure.
8,8'-(1,4,10,13-Tetrathia-7,16-diazacyclooctadecane-7,16-diyl)-bis(methylene)diquinolin-7-ol (TTBQ) was synthesized and proved to selectively recognize Hg(2+), forming a Hg(2+)/TTBQ complex with an association constant (K(a)) as large as approximately 1.3 x 10(4) +/- 520 M(-1) in aqueous solution. Single crystals of TTBQ and a TTBQ-Hg(2+) complex have also been successfully grown, in which the latter unambiguously revealed a cagelike configuration consisting of thiol-crown and dual 7-hydroxyquinoline moieties to firmly trap Hg(2+). This 3D-complexation structure accounts for approximately 25-fold luminescence enhancement and a detection limit of sub-microM in water for sensing Hg(2+). Great selectivity toward Hg(2+) has been exhibited over alkali- and alkaline-earth metal ions, first-row transition-metal ions, and other cations studied. This chemosensor is particularly suited for the detection of Hg(2+) in a pH range of 5.5 to 7.5. This, in combination with its fine biocompatibility, leads to the success toward in vitro mercury recognition based on fluorescence imaging.
Department of Chemistry, Soochow University, Taipei, Taiwan. meilin_ho@scu.edu.tw
Mei-Lin
ML
Ho
Kew-Yu
KY
Chen
Gene-Hsiang
GH
Lee
Yu-Chun
YC
Chen
Chih-Chieh
CC
Wang
Jyh-Fu
JF
Lee
Wen-Chun
WC
Chung
Pi-Tai
PT
Chou
D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D018360:Crystallography, X-Ray; D005453:Fluorescence; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000737:chemistry*; D015394:Molecular Structure; D011804:Quinolines / Q000737:chemistry*; D013057:Spectrum Analysis
C530509:8,8'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)-bis(methylene)diquinolin-7-ol; D006573:Heterocyclic Compounds, 1-Ring; D011804:Quinolines; D008628:Mercury