Expression of the AMACO (VWA2 protein) ortholog in zebrafish.
AMACO is a basement membrane associated protein that belongs to the VWA domain-containing protein superfamily. In addition to three VWA domains it contains two EGF-like domains, a cysteine-rich domain and a unique domain. Mouse AMACO has been partially characterized, but its function remains unknown. The zebrafish genome contains a single AMACO ortholog gene on chromosome 12. The domain structure is completely conserved between zebrafish and mouse and the first EGF-like domain, carrying a rare O-glucosylation and O-fucosylation consensus sequence, has the highest identity at the protein level. RT-PCR shows strongest AMACO expression during development, starting at the 5 somite stage. An antibody specific for zebrafish AMACO detected expression mainly in myosepta but also in skin, pronephros, pituitary gland, otic capsule and gills. In situ hybridization revealed that the muscle precursor cells of the somites express the protein that is laid down in the myosepta.
Gebauer
Jan M
JM
Center for Biochemistry, University of Cologne, D-50931 Cologne, Germany.
Karlsen
Kristina Røkenes
KR
Neiss
Wolfram F
WF
Paulsson
Mats
M
Wagener
Raimund
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005880:Gills / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012867:Skin / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D016326:Extracellular Matrix Proteins; C549398:VWA2 protein, zebrafish; D029961:Zebrafish Proteins