Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays.
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
Roche Diagnostics GmbH, Mannheim, Germany.
Hanne
H
Zimmermann
Daniel
D
Gerhard
Theo
T
Dingermann
Ludwig A
LA
Hothorn
D001681:Biological Assay / Q000379:methods*; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D007118:Immunoassay / Q000379:methods*; D016014:Linear Models; D008954:Models, Biological; D015233:Models, Statistical; D017711:Nonlinear Dynamics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity