Genetic variability and phylogenetic analysis of hosta virus X.
Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3-100% and 98.2-100% nucleotide and amino acid sequence identity, respectively, whereas TGB1 shared 97.4-100% nucleotide and 97-100% amino acid sequence identity. TGB1 of TN isolates were all longer by one codon from that of a Korean isolate, which is the only sequence publicly available. Phylogenetic analysis of nucleotide and amino acid sequences of TGB1 and CP of all known HVX isolates, separately or combined, revealed a close relationship, suggesting that all of them are derived from a common ancestor. Phylogenetic analysis with the type member of each genus of the family Flexiviridae confirmed that HVX is a member of a distinct species of the genus Potexvirus.
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA.
O L
OL
Fajolu
R-H
RH
Wen
A S
AS
Windham
M T
MT
Windham
J K
JK
Moulton
M R
MR
Hajimorad
D036022:Capsid Proteins / Q000235:genetics; D014644:Genetic Variation; D031422:Hosta / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013714:Tennessee; D014764:Viral Proteins / Q000235:genetics
D036022:Capsid Proteins; D014764:Viral Proteins