Amyloid-beta colocalizes with apolipoprotein B in absorptive cells of the small intestine.
BACKGROUND
Amyloid-beta is recognized as the major constituent of senile plaque found in subjects with Alzheimer's disease. However, there is increasing evidence that in a physiological context amyloid-beta may serve as regulating apolipoprotein, primarily of the triglyceride enriched lipoproteins. To consider this hypothesis further, this study utilized an in vivo immunological approach to explore in lipogenic tissue whether amyloid-beta colocalizes with nascent triglyceride-rich lipoproteins.


RESULTS
In murine absorptive epithelial cells of the small intestine, amyloid-beta had remarkable colocalization with chylomicrons (Manders overlap coefficient = 0.73 +/- 0.03 (SEM)), the latter identified as immunoreactive apolipoprotein B. A diet enriched in saturated fats doubled the abundance of both amyloid-beta and apo B and increased the overlap coefficient of the two proteins (0.87 +/- 0.02). However, there was no evidence that abundance of the two proteins was interdependent within the enterocytes (Pearson's Coefficient < 0.02 +/- 0.03), or in plasma (Pearson's Coefficient < 0.01).


CONCLUSIONS
The findings of this study are consistent with the possibility that amyloid-beta is secreted by enterocytes as an apolipoprotein component of chylomicrons. However, secretion of amyloid-beta appears to be independent of chylomicron biogenesis.
The Australian Technology Network Centre for Metabolic Fitness, School of Public Health, Curtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia, Australia. susan.galloway@postgrad.curtin.edu.au
Susan
S
Galloway
Ryusuke
R
Takechi
Menuka M S
MM
Pallebage-Gamarallage
Satvinder S
SS
Dhaliwal
John C L
JC
Mamo
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism*; D002914:Chylomicrons / Q000096:biosynthesis; D004041:Dietary Fats; D020895:Enterocytes / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000737:chemistry*; D008074:Lipoproteins; D051379:Mice; D014280:Triglycerides
D016229:Amyloid beta-Peptides; D001055:Apolipoproteins B; D002914:Chylomicrons; D004041:Dietary Fats; D008074:Lipoproteins; D014280:Triglycerides; C030087:lipoprotein triglyceride