[Expression of caspase-3 in rat kidney with renal tubular damage induced by lipopolysaccharide and hypoxia].
OBJECTIVE
To observe the expression of caspase-3 in the kidney of a rat model of renal tubular damage induced by endotoxin and hypoxia and explore the mechanism of renal tubular damage.


METHODS
Ten rats were anesthetized with artificial ventilation and received 2 mg/kg lipopolysaccharide (LPS) injection through the penile vein. The FiO2 was reduced 90 min later from 21% to 5%, and the ventilation was withdrawn after another 90 min. Immediately after ventilation withdrawal, the kidney of the rats were obtained for immunocytochemistry and HE staining.


RESULTS
HE staining showed obvious hyperemia in most of the glomeruli, mild swelling of the endothelial and mesangial cells, severe swelling and turbidity in the proximal tubular epithelial cells without obvious changes in most of the distal proximal tubules. A small portion of the interstitial epithelial cells showed swelling and turbidity, and the entire renal interstitium appeared hyperemic but without inflammatory cell infiltration. Immunocytochemistry detected the presence of caspase-3 in the cytoplasm, and most of the distal renal tubule cells were positive for caspase-3, while only occasional cells showed caspase-3 positivity in the proximal tubular epithelial cells. Most of the proximal tubular epithelial and glomerulus cells were negative for caspase-3.


CONCLUSIONS
Endotoxin and hypoxia can induce renal damage, particularly in the proximal renal tubule cells, and the distal tubular epithelial cells sustain relatively light damage. Caspase-3 is strongly expressed in the distal renal tubular cells, suggesting that in renal tubular damage induced by endotoxin and hypoxia, cell degeneration, necrosis and apoptosis coexist in the tubular epithelial cells; degeneration and necrosis occur primarily in the proximal tubular epithelial cells, while apoptosis is obvious in the distal renal cells.
Department of Pediatrics, First Affiliated Hospital, Medical College of Jinan University, Guangzhou 510630, China. tyf@jnu.edu.cn
Fang
F
Yang
Guo-sheng
GS
Liu
Xiao-ye
XY
Lu
Jiu-ling
JL
Kang
D000818:Animals; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D000860:Hypoxia / Q000139:chemically induced*; D007668:Kidney / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology*; D008070:Lipopolysaccharides; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; C505410:Casp3 protein, rat; D053148:Caspase 3