Saliva substitute in combination with high-concentrated fluoride toothpaste: effects on demineralised dentin in vitro.
OBJECTIVE
The aim of this in vitro study was to assess the effects of saliva substitutes (modified with respect to calcium, phosphates, and fluorides) in combination with a high-concentrated fluoride toothpaste on demineralised dentin.


METHODS
Before and after demineralisation of bovine dentin specimens (subsurface lesions; 37 degrees C, pH 5.0, 5 d), one-quarter of each specimen's surface was covered with nail varnish (control of sound/demineralised tissue). Subsequently, specimens were exposed to original Saliva natura (saturation with respect to octacalciumphosphate [S(OCP)]: 0.03; SN 0), or to three lab-produced Saliva natura modifications (S(OCP): 1, 2, and 3; SN 1-3) for 2 and 5 weeks (37 degrees C). An aqueous solution (S(OCP): 2.5) served as positive control (PC). Two times daily (2min each), Duraphat toothpaste (5000ppmF(-); Colgate)/saliva substitute slurry (ratio 1:3) was applied gently. Differences in mineral losses (DeltaDeltaZ) and lesion depths (DeltaLD) between values before and after exposure were microradiographically evaluated.


RESULTS
After both treatment periods specimens immersed in SN 0 revealed significantly higher mineral losses (lower DeltaDeltaZ values) and lesion depths (lower DeltaLD) compared to PC (p<0.05; ANOVA). After 5 weeks, specimens stored in SN 1 and 2 showed significantly higher mineral losses compared to PC (p<0.05), while those stored in SN 3 showed similar results (p>0.05). No differences in mineral loss could be observed between SN 2 and 3 (p>0.05).


CONCLUSIONS
Under the conditions of this limited protocol, the combination of Saliva natura solutions slightly saturated with respect to OCP in combination with a high-concentrated fluoride toothpaste enabled remineralisation of dentin in vitro.
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, CharitéCentrum 3, Charité - Universitätsmedizin Berlin, Germany. peter.tschoppe@charite.de
Peter
P
Tschoppe
Daniela L
DL
Zandim
José E C
JE
Sampaio
Andrej M
AM
Kielbassa
D000704:Analysis of Variance; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology / Q000627:therapeutic use*; D002327:Cariostatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002417:Cattle; D003804:Dentin / Q000187:drug effects; D004338:Drug Combinations; D012464:Saliva, Artificial / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012969:Sodium Fluoride / Q000494:pharmacology / Q000627:therapeutic use*; D018709:Statistics, Nonparametric; D017001:Tooth Demineralization / Q000188:drug therapy*; D014089:Tooth Remineralization / Q000379:methods*; D014100:Toothpastes / Q000737:chemistry / Q000627:therapeutic use
D002130:Calcium Phosphates; D002327:Cariostatic Agents; D004338:Drug Combinations; D012464:Saliva, Artificial; D014100:Toothpastes; C022045:octacalcium phosphate; D012969:Sodium Fluoride