Early morphological and histochemical alterations in rats subjected to ileocystoplasty.
OBJECTIVE
To study morphologic and histochemical alterations arising at the ileocystoplasty site.


METHODS
Sixteen Wistar female rats were subjected to ileocystoplasty and sacrificed after eight weeks. Material collected was divided into four groups for histological and histochemical studies: Group I (control) - isolated ileum segment removed during ileocystoplasty; Group II - ileoileal anastomosis; Group III - ileovesical anastomosis and Group IV - ileal segment from the neobladder. Histological and histochemical study assessed dysplasia, metaplasia, acute and chronic inflammation, fibrosis, atrophy, hypertrophy, total mucins, sialomucins and sulfomucins. The non-parametric Wilcoxon and Mann-Whitney tests were employed in statistical analysis.


RESULTS
None of the groups presented dysplasia. Acute inflammation and atrophy occurred in Groups II, III and IV, not reaching statistical significance. Metaplasia was significant only in Group III (p=0.012). Chronic inflammation, fibrosis and hypertrophy were significant in Groups II, III and IV. There was a significant increase in total mucin content in Group IV (p=0.014) and a reduction in Group III (p=0.016). Increases in sialomucins were observed in samples for Groups III (p=0.003) and IV (p=0.002) along with reduced sulfomucins in samples from Groups III (p=0.013) and IV (p=0.008).


CONCLUSIONS
Ileocystoplasty in female rats caused squamous metaplasia, chronic inflammatory infiltration, fibrosis, hypertrophy, increase in sialomucin content, reduction in sulfomucins, and alterations in total mucin content with statistical significance, as well acute inflammatory infiltration and muscular atrophy with less intensity.
Miranda
Edinaldo Gonçalves de
EG
Head Division of Pediatric Surgery and Experimental Surgery, FACIME, UESPI, Piaui, Brazil. edinaldomiranda@hotmail.com
Bizerra
Marcelo Ribeiro de Sousa
MR
Waisberg
Daniel Reis
DR
Carnevale
José
J
Silva
José Figueredo
JF
Waisberg
Jaques
J
D000714:Anastomosis, Surgical; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007082:Ileum / Q000473:pathology / Q000601:surgery*; D008679:Metaplasia; D051381:Rats; D017208:Rats, Wistar; D052116:Sialomucins / Q000032:analysis; D001743:Urinary Bladder / Q000473:pathology / Q000601:surgery*; D001745:Urinary Bladder Diseases / Q000601:surgery*; D014547:Urinary Diversion / Q000009:adverse effects / Q000379:methods
D052116:Sialomucins