Adaptive harmonic generation microscopy of mammalian embryos.
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.
Jesacher
Alexander
A
1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
Thayil
Anisha
A
Grieve
Kate
K
Débarre
Delphine
D
Watanabe
Tomoko
T
Wilson
Tony
T
Srinivas
Shankar
S
Booth
Martin
M
D000388:Air; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods; D004622:Embryo, Mammalian / Q000378:metabolism*; D004867:Equipment Design; D005898:Glass; D007091:Image Processing, Computer-Assisted; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D012031:Refractometry / Q000379:methods; D013997:Time Factors