Typing of Pseudomonas aeruginosa isolated from patients with VAP in an intensive care unit.
Aim of this study was to characterize isolates of Pseudomonas aeruginosa responsible for ventilator-associated pneumonia (VAP) in patients admitted to an ICU in order to evaluate a possible strain clonality. The study was performed from October 2004 to June 2005 in one Southern Italy ICU and 29 patients suspected of having VAP were enrolled. The etiology of VAP was established by quantitative cultures of endotracheal aspirations. Molecular characterization was carried out by PFGE. P. aeruginosa was responsible for 51% of all cases of VAP (15/29) and 12/15 strains were multi-drug resistant. High mortality (44.8%) was connected to this pathogen and evidence of strain clonality was found. The early identification of strain clonality and the application of infection control procedures are necessary to avoid the spread of pathogens such as P. aeruginosa involved in nosocomial infections.
Lambiase
Antonietta
A
Department of Cellular and Molecular Biology and Pathology "Luigi Califano", Medicine School, University "Federico II", Naples, Italy. alambias@unina.it
Rossano
Fabio
F
Piazza
Ornella
O
Del Pezzo
Mariassunta
M
Catania
Maria Rosaria
MR
Tufano
Rosalba
R
D000368:Aged; D000890:Anti-Infective Agents / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007362:Intensive Care Units; D007558:Italy / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D053717:Pneumonia, Ventilator-Associated / Q000453:epidemiology / Q000209:etiology*; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
D000890:Anti-Infective Agents; D004269:DNA, Bacterial