Neucrin is a novel neural-specific secreted antagonist to canonical Wnt signaling.
A gene encoding a novel secreted protein in mice and humans was identified, and named Neucrin. Mouse Neucrin consists of 343 amino acids with a cysteine-rich domain in its carboxyl terminal region. The positions of 10 cysteine residues in the cysteine-rich domain are similar to those of Dickkopfs (Dkks), secreted Wnt antagonists. However, whereas Dkks have two cysteine-rich domains, Neucrin has only one. Neucrin as well as Dkks bound to LDL receptor-related protein 6 and inhibited the stabilization of cytosolic beta-catenin, indicating that Neucrin is an antagonist of canonical Wnt signaling. Mouse Neucrin expression was not detected in any major tissues in the adult, but was detected in developing neural tissues, including the brain and spinal cord. The expression pattern of Neucrin is distinct from that of any Dkk. Neucrin is a unique secreted Wnt antagonist that is predominantly expressed in developing neural tissues.
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo, Kyoto 606-8501, Japan. miyakea@pharm.kyoto-u.ac.jp
Ayumi
A
Miyake
Yohsuke
Y
Takahashi
Hiroyuki
H
Miwa
Akihiko
A
Shimada
Morichika
M
Konishi
Nobuyuki
N
Itoh
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011973:Receptors, LDL / Q000378:metabolism; D051153:Wnt Proteins / Q000037:antagonists & inhibitors*; D051176:beta Catenin / Q000378:metabolism
C545900:DRAXIN protein, human; D036341:Intercellular Signaling Peptides and Proteins; C555475:LRP6 protein, human; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555476:Lrp6 protein, mouse; D009419:Nerve Tissue Proteins; D011973:Receptors, LDL; D051153:Wnt Proteins; D051176:beta Catenin; C534831:draxin protein, mouse; D003545:Cysteine