Preparation and time-resolved luminescence bioassay application of multicolor luminescent lanthanide nanoparticles.
Because highly luminescent lanthanide compounds are limited to Eu(3+) and Tb(3+) compounds with red (Eu, approximately 615 nm) and green (Tb, approximately 545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu(3+)-Tb(3+) complexes with a ligand N,N,N(1),N(1)-(4'-phenyl-2,2':6',2''-terpyridine-6,6''-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.
Jiang
Hongfei
H
State Key Laboratory of Fine Chemicals, Department of Chemistry, Dalian University of Technology, Dalian, 116012, People's Republic of China.
Wang
Guilan
G
Zhang
Wenzhu
W
Liu
Xiaoyu
X
Ye
Zhiqiang
Z
Jin
Dayong
D
Yuan
Jingli
J
Liu
Zhiguang
Z
D000085:Acetates / Q000737:chemistry; D000818:Animals; D003116:Color; D005470:Fluorometry; D016829:Giardia lamblia / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging; D010316:Particle Size; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000276:immunology; D012822:Silicon Dioxide / Q000737:chemistry; D019809:Streptavidin / Q000378:metabolism; D013997:Time Factors
D000085:Acetates; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D012822:Silicon Dioxide; D019809:Streptavidin; D017430:Prostate-Specific Antigen