Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer films--effects on fibroblast spreading.
The Pro-His-Ser-Arg-Asn (PHSRN) sequence in fibronectin is a second cell-binding site that synergistically affects Arg-Gly-Asp (RGD). The PHSRN peptide also induces cell invasion and accelerates wound healing. We report on the surface immobilization of PHSRN by spontaneous adsorption on polysiloxane thin films which have different surface free energy characteristics. Low-surface energy (hydrophobic) polysiloxane and the corresponding high-surface energy (hydrophilic) surfaces obtained by UV-ozone treatments were used as adsorbing substrates. The peptide adsorption process was investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy. Both adsorption kinetics and peptide rearrangement dynamics at the solid interface were significantly different on the surface-modified films compared to the untreated ones. Fibroblast cells cultures at short times and in a simplified environment, i.e., a medium-free solution, were prepared to distinguish interaction events at the interface between cell membrane and surface-immobilized peptide for the two cases. It turned out that the cell-adhesive effect of immobilized PHSRN was different for hydrophobic compared to hydrophilic ones. Early signatures of cell spreading were only observed on the hydrophilic substrates. These effects are explained in terms of different spatial arrangements of PHSRN molecules immobilized on the two types of surfaces.
Department of Chemical Sciences, Catania University, viale A. Doria 6, 95125 Catania, Italy. csatriano@unict.it
Cristina
C
Satriano
Grazia M L
GM
Messina
Clara
C
Marino
Ivana
I
Aiello
Enrico
E
Conte
Diego
D
La Mendola
Donatella A
DA
Distefano
Franca
F
D'Alessandro
Giuseppe
G
Pappalardo
Giuseppe
G
Impellizzeri
D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002942:Circular Dichroism; D005347:Fibroblasts / Q000166:cytology*; D005353:Fibronectins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008853:Microscopy; D018625:Microscopy, Atomic Force; D010126:Ozone / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects; D011487:Protein Conformation; D012833:Siloxanes / Q000737:chemistry; D013499:Surface Properties; D013816:Thermodynamics; D054457:Tissue Scaffolds / Q000737:chemistry; D014466:Ultraviolet Rays; D017155:Wettability
D005353:Fibronectins; D010446:Peptide Fragments; D011108:Polymers; D012833:Siloxanes; C515054:poly(hydroxymethylsiloxane); C495978:prolyl-histidyl-seryl-arginyl-asparagine; D010126:Ozone