MicroRNA-214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells.
MicroRNAs are a group of endogenously expressed, single-stranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.
Yang
Zuozhen
Z
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
Chen
Shuang
S
Luan
Xuejing
X
Li
Yixuan
Y
Liu
Min
M
Li
Xin
X
Liu
Tao
T
Tang
Hua
H
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D048371:MAP Kinase Kinase 3 / Q000096:biosynthesis; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D008875:Middle Aged; D048055:Mitogen-Activated Protein Kinase 8 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D048055:Mitogen-Activated Protein Kinase 8; D048371:MAP Kinase Kinase 3; C482530:MAP2K3 protein, human