Studies on the synthesis of basic proteins and antigenic components in measles virus infected cells.
The synthesis of histone f1 fraction in synchronous culture of (3H)-L-lysine labeled HeLa cells was inhibited 30 to 45 hours earlier and to a higher degree after infection with attenuated L-16 strain of the measles virus than after infection with laboratory Edmonston strain. Dithiothreitol and triton X-100 were used to isolate a cytoplasmatic component (K-2c) lacking infectivity but associated with hemagglutinating activity, from both strains. The component from both strain induced the synthesis of measles antibodies detected by the hemagglutination inhibition, neutralization and immunodiffusion techniques. The antibody titers in the sera of animals vaccinated with K-2c of L-16 strain were similar or higher than for K-2c of the Edmonston strain.
Department of Virology, Department of Radiation Protection and Radiobiology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
I
I
Polna
F
F
Rzendowska
J
J
Beker
G
G
Hirszfeld
W
W
Sadowski
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D006168:Guinea Pigs; D006367:HeLa Cells; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006657:Histones / Q000096:biosynthesis*; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D008458:Measles Vaccine / Q000276:immunology; D008459:Measles virus / Q000254:growth & development*; D009500:Neutralization Tests; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006657:Histones; D008458:Measles Vaccine; D014764:Viral Proteins