Glycerol conversion to D-xylulose by a two-stage microbial reaction using Candida parapsilosis and Gluconobacter oxydans.
A yeast strain, 25N-2B, that produces D-arabitol from glycerol, was identified as Candida parapsilosis based on phylogenetic, morphological, physiological, and biochemical analyses. It produced 32.2 g/L D-arabitol from 170 g/L glycerol in a jar fermentor. The D-arabitol in the reaction mixture was then completely converted to D-xylulose using Gluconobacter oxydans NBRC3293. The product was isolated from the reaction mixture and confirmed to be D-xylulose by (1)H and (13)C-NMR and optical rotation.
Habe
Hiroshi
H
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
Fukuoka
Tokuma
T
Kitamoto
Dai
D
Sakaki
Keiji
K
D002175:Candida / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D020600:Gluconobacter oxydans / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry* / Q000378:metabolism; D010802:Phylogeny; D014996:Xylulose / Q000737:chemistry* / Q000378:metabolism
D014996:Xylulose; D005990:Glycerol