Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom.
Crotoxin B is a basic phospholipase A(2) found in the venom of several Crotalus durissus ssp. rattlesnakes and is one of the subunits that constitute crotoxin, the main component of the venom of these snakes. This heterodimeric toxin is related to important envenomation effects such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, the first structural data only became available in 2007 (for crotoxin B from C. durissus terrificus) and showed an ambiguous result for the biological assembly, which could be either dimeric or tetrameric. In this work, the crystallization, X-ray diffraction data collection at 2.2 A resolution and molecular-replacement solution of a dimeric complex formed by two crotoxin B isoforms from C. durissus collilineatus venom is presented.
Departamento de Física e Biofísica, Instituto de Biociências, UNESP, Distrito de Rubião Júnior, Caixa Postal 510, 18618-000 Botucatu-SP, Brazil.
G H M
GH
Salvador
C A H
CA
Fernandes
L C
LC
Corrêa
N A
NA
Santos-Filho
A M
AM
Soares
M R M
MR
Fontes
D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry; D003439:Crotoxin / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D054467:Phospholipases A2 / Q000737:chemistry; D020836:Protein Structure, Quaternary
D003435:Crotalid Venoms; D003439:Crotoxin; D054467:Phospholipases A2