Suppressor of Hairless is required for long-term memory formation in Drosophila.
Suppressor of Hairless [Su(H)] is a DNA-binding protein of the Notch-signaling pathway, which is important for developmental processes and has been implicated in behavior plasticity. It acts as a transcriptional activator in the Notch pathway, but also as a repressor in the absence of Notch signaling. Our previous work has shown that Notch signaling contributes to long-term memory formation in the Drosophila adult brain. In the present report, we show that Su(H) null heterozygous mutants perform normally for learning, early memory, and anesthesia-resistant memory, whereas long-term memory is impaired. Interestingly, we find overexpressing wild- type Su(H) also causes long-term memory defect in Drosophila. Significantly, induction of a heat-shock inducible Su(H)(+) transgene before training can fully rescue the memory defect of Su(H) mutants, thereby demonstrating an acute role for Su(H) in behavioral plasticity. We show that Su(H) is widely expressed in the adult brain. Transgenic expression of wild-type Su(H) in the Mushroom Bodies is sufficient to rescue the memory defect of Su(H) mutants. Our data clearly demonstrate that transcriptional activity of Su(H) in Notch signaling in the mushroom bodies is critical for the formation of long-term memory.
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Qingxuan
Q
Song
Kan
K
Sun
Yichun
Y
Shuai
Ran
R
Lin
Wenjia
W
You
Lianzhang
L
Wang
Yi
Y
Zhong
D000818:Animals; D030801:Animals, Genetically Modified; D003214:Conditioning, Classical / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D006361:Heating / Q000379:methods; D007859:Learning Disabilities / Q000235:genetics / Q000378:metabolism; D057567:Memory, Long-Term / Q000502:physiology*; D024521:Mushroom Bodies / Q000502:physiology; D009154:Mutation / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000172:deficiency / Q000378:metabolism*; D012903:Smell / Q000235:genetics; D013997:Time Factors
D029721:Drosophila Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C088731:Su(H) protein, Drosophila