Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis.
The wound response prohormone jasmonic acid (JA) accumulates rapidly in tissues both proximal and distal to injury sites in plants. Using quantitative liquid chromatography-mass spectrometry after flash freezing of tissues, we found that JA accumulated within 30 s of injury in wounded Arabidopsis leaves (p = 3.5 e(-7)). JA augmentation distal to wounds was strongest in unwounded leaves with direct vascular connections to wounded leaves wherein JA levels increased significantly within 120 s of wounding (p = 0.00027). This gave conservative and statistically robust temporal boundaries for the average velocity of the long distance signal leading to distal JA accumulation in unwounded leaves of 3.4-4.5 cm min(-1). Like JA, transcripts of the JA synthesis gene LIPOXYGENASE2 (LOX2) and the jasmonate response gene JAZ10.3 also accumulated to higher levels in directly interconnected leaves than in indirectly connected leaves. JA accumulation in a lox2-1 mutant plant was initiated rapidly after wounding then slowed progressively compared with the wild type (WT). Despite this, JAZ10.3 expression in the two genotypes was similar. Free cyclopentenone jasmonate levels were similar in both resting WT and lox2-1. In contrast, bound cyclopentenone jasmonates (arabidopsides) were far lower in lox2-1 than in the WT. The major roles of LOX2 are to generate arabidopsides and the large levels of JA that accumulate proximal to the wound. LOX2 is not essential for some of the most rapid events elicited by wounding.
Glauser
Gaetan
G
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Dubugnon
Lucie
L
Mousavi
Seyed A R
SA
Rudaz
Serge
S
Wolfender
Jean-Luc
JL
Farmer
Edward E
EE
D000818:Animals; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism / Q000469:parasitology; D015398:Signal Transduction / Q000502:physiology*; D018411:Spodoptera / Q000378:metabolism
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C525049:JAS1 protein, Arabidopsis; D009687:Nuclear Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D008084:Lipoxygenase