[Follicular cell implantation for induce hair follicle growth in nude mice].
OBJECTIVE
To establish a convenient and reliable method for inducing hair regeneration by follicular cell implantation for the treatment of alopecia.


METHODS
The human hair follicle stem cells were separated and purified by micromanipulation and magnetic cell sorting, and human scalp dermal papilla cells were isolated by enzyme digestion. The two cells were mixed and implanted subcutaneously in nude mice to observe the regeneration of the hair follicles.


RESULTS
Formation of intact hair follicle-like structures was observed in the skin sections of the recipient nude mice.


CONCLUSIONS
Follicular cell implantation can induce hair follicle-like structures in nude mice, which provides a means for efficient hair regeneration for treatment of hair loss.
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. dr.tanting@hotmail.com
Ting
T
Tan
Zhi-qi
ZQ
Hu
D000328:Adult; D000505:Alopecia / Q000601:surgery; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D018859:Hair Follicle / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous; D055815:Young Adult