Monitoring gene expression of potato under salinity using cDNA microarrays.
The molecular response to salt exposure was studied in the leaves of a Solanum tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed. On the other hand, we observed the induction of various kinds of transcription factors implicated in osmotic stress response via ABA-dependent or ABA-independent pathways but also in plant defense pathways. This revealed a crosstalk between abiotic and biotic stress responses during salt exposure, which activated several adaptation mechanisms including heat shock proteins, late embryogenesis abundant, dehydrins and PR proteins. Gene expression changes related to carbohydrate and amino acid metabolism were also observed, pointing at putative modifications at the metabolic level.
Legay
Sylvain
S
Department EVA Environment and Agrobiotechnologies, Centre de Recherche Public-Gabriel Lippmann, 4422 Belvaux, Luxembourg.
Lamoureux
Didier
D
Hausman
Jean-François
JF
Hoffmann
Lucien
L
Evers
Danièle
D
D001692:Biological Transport / Q000187:drug effects; D002241:Carbohydrates / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D014674:Plant Proteins, Dietary / Q000494:pharmacology; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D012987:Soil; D011198:Solanum tuberosum / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D002241:Carbohydrates; D002364:Caseins; D008055:Lipids; D014674:Plant Proteins, Dietary; D012987:Soil; C080652:Osmolite; D012965:Sodium Chloride