[2-(14)C-Methylerythritol 2,4-cyclodiphosphate incorporation into bacterial and plant isoprenoids].
To work out a test system for studying the inhibition of isoprenoid biosynthesis through non-mevalonate pathway, the conversion of 2-(14)C-methylerythritol 2,4-cyclodiphosphate (14C-MEC) into isoprenoids of three bacteria species and into plastids of 11 higher plant species was studied. The highest efficiency (up to 63%) of the process was observed with chromoplasts from narcissus (Narcissus pseudonarcissus L.) and celandine (Chelidonium majus L.). Twenty five percent of added 14C-MEC was recovered in an isoprenoid fraction of red pepper (Capsicum annuum L.) chromoplasts. Thus, these three models can be used to test antibiotic inhibitors of isoprenoid biosynthesis.
K V
KV
Mazikin
Iu V
IuV
Ershov
A V
AV
goncharenko
D N
DN
Ostrovskiĭ
D001419:Bacteria / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism / Q000494:pharmacology; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010944:Plants / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism* / Q000494:pharmacology; D013729:Terpenes / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; D002247:Carbon Isotopes; D013403:Sugar Phosphates; D013729:Terpenes; D004896:Erythritol