Acinetobacter sp. Ud-4 efficiently degrades both edible and mineral oils: isolation and characterization.
A novel Acinetobacter strain, Ud-4, possessing a strong capacity to degrade edible, lubricating, and heavy oil was isolated from seawater in a fishing port located in Toyama, Japan. It was identified by morphological and physiological analyses and 16S rDNA sequencing. This strain could utilize five types of edible oils (canola oil, olive oil, sesame oil, soybean oil, and lard), lubricating oil, and C-heavy oil as the sole carbon source for growth in M9 medium. The strain grew well and heavily degraded edible oils in Luria-Bertani medium during a 7-day culture at 25 degrees C; it also degraded all kinds of oils in artificial seawater medium for marine bacteria. Furthermore, this strain was capable of degrading almost all C10-C25 n-alkanes in C-heavy oil during a 4-week culture. Oligonucleotide primers specific to two catabolic genes involved in the degradation of n-alkanes (Acinetobacter sp. alkM) and triglyceride (Acinetobacter sp. lipA) allowed amplification of these genes in strain Ud-4. To our knowledge, this is the first report on the isolation of a bacterium that can efficiently degrade both edible and mineral oils.
Graduate School of Science and Engineering, University of Toyama, Toyama, Japan. tanakada@sci.u-toyama.ac.jp
Daisuke
D
Tanaka
Miyuki
M
Takashima
Asako
A
Mizuta
Shunsuke
S
Tanaka
Akihiro
A
Sakatoku
Atsushi
A
Nishikawa
Tsutomu
T
Osawa
Munenori
M
Noguchi
Shin-Ichi
S
Aizawa
Shogo
S
Nakamura
D000150:Acinetobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D000473:Alkanes / Q000378:metabolism; D001673:Biodegradation, Environmental; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D007564:Japan; D007700:Kinetics; D054327:Lubricants / Q000378:metabolism; D008854:Microscopy, Electron; D008899:Mineral Oil / Q000378:metabolism*; D009821:Oils / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D014280:Triglycerides / Q000378:metabolism
D000473:Alkanes; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D054327:Lubricants; D009821:Oils; D012336:RNA, Ribosomal, 16S; D014280:Triglycerides; D008899:Mineral Oil