Decreased polyunsaturated Fatty Acid content contributes to increased survival in human colon cancer.
Among diet components, some fatty acids are known to affect several stages of colon carcinogenesis, whereas others are probably helpful in preventing tumors. In light of this, our aim was to determine the composition of fatty acids and the possible correlation with apoptosis in human colon carcinoma specimens at different Duke's stages and to evaluate the effect of enriching human colon cancer cell line with the possible reduced fatty acid(s). Specimens of carcinoma were compared with the corresponding non-neoplastic mucosa: a significant decrease of arachidonic acid, PPARalpha, Bad, and Bax and a significant increase of COX-2, Bcl-2, and pBad were found. The importance of arachidonic acid in apoptosis was demonstrated by enriching a Caco-2 cell line with this fatty acid. It induced apoptosis in a dose- and time-dependent manner via induction of PPARalpha that, in turn, decreased COX-2. In conclusion, the reduced content of arachidonic acid is likely related to carcinogenic process decreasing the susceptibility of cancer cells to apoptosis.
Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, 10125 Torino, Italy.
Manuela
M
Oraldi
Antonella
A
Trombetta
Fiorella
F
Biasi
Rosa A
RA
Canuto
Marina
M
Maggiora
Giuliana
G
Muzio