Expression of M-CSF and CSF-1R is correlated with histological grade in soft tissue tumors.
BACKGROUND
Macrophage colony stimulating factor (M-CSF) binds to colony-stimulating factor-1 receptor (CSF-1R) and thereby stimulates the proliferation, differentiation and behaviour of monocytes, macrophages and their bone marrow progenitors. Previous studies have suggested that high expression of these markers is correlated with poor prognosis.


METHODS
M-CSF, CSF-1R and CD68 protein expression was examined by immunohistochemistry in paraffin embedded sections of soft tissue tumor specimens from 46 patients. The proportion of positive cells and the expression intensity of M-CSF, CSF-1R and CD68 in both the tumor cell areas and the adjacent stromal areas were correlated to the histological grade.


RESULTS
In the high grade tumors M-CSF and CSF-1R were more highly expressed than in the low grade tumors. This was seen in both the tumor cell areas and the adjacent stromal areas. No differences in CD68 expression between the high and low grade tumors were found either in the tumor cell areas or the stromal areas.


CONCLUSIONS
The expression of M-CSF and CSF-1R in tumor cell areas and adjacent stromal areas correlate with the histological grade of soft tissue tumors.
Department of Pathology, Post Box 46, University Hospital of Northern Norway, 9038 Tromsø, Norway. elin.richardsen@unn.no
Elin
E
Richardsen
Sveinung Wergeland
SW
Sørbye
John Phil
JP
Crowe
Jia-Lin
JL
Yang
Lill-Tove
LT
Busund
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D012509:Sarcoma / Q000378:metabolism* / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor; C067980:CD68 antigen, human; D000097382:CD68 Molecule