Teladorsagia circumcincta: survival of adults in vitro is enhanced by the presence of a mammalian cell line.
Adult Teladorsagia circumcincta survival and motility in vitro was examined in a range of different cell culture media, supplements and gas mixes. Under optimum conditions, worms survived for 14 days, exhibiting high motility for 9 days and egg production for 72 h. Optimum conditions involved co-culture of worms with a HeLa cell line in a supplemented cell medium (CEM) and an atmosphere containing 10% CO(2), 5% O(2) 85% N(2), 65% humidity at 37 degrees C. The incubation medium consisted of Minimum Essential Medium with 10% fetal calf serum, 1% non-essential amino acids, 1% glutamax and 1% penicillin-neomycin-streptomycin cocktail mix. Compared with optimum conditions, incubation in CEM alone, cell conditioned CEM, RPMI alone, Medium 199 alone, reduced CO(2) or O(2), or when cells were replaced with Escherichia coli, both survival and motility were reduced. Optimum conditions for adult T. circumcincta maintenance for culture, anthelmintic testing or generation of excretory/secretory products are described.
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11222, Palmerston North 5301, New Zealand.
A
A
Luque
L R
LR
Walker
J C
JC
Pedley
K C
KC
Pedley
K
K
Hillrichs
H V
HV
Simpson
D C
DC
Simcock
D000818:Animals; D002245:Carbon Dioxide; D002460:Cell Line; D018920:Coculture Techniques; D003470:Culture Media; D017077:Culture Media, Conditioned; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D009068:Movement; D009584:Nitrogen; D010058:Oviposition; D010100:Oxygen; D012756:Sheep; D013997:Time Factors; D014251:Trichostrongyloidea / Q000254:growth & development* / Q000502:physiology
D003470:Culture Media; D017077:Culture Media, Conditioned; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen