Evaluation of in vitro fertilization parameters and estrogen receptor alpha gene polymorphisms for women with unexplained infertility.
OBJECTIVE
Association of ESR1 gene PvuII, XbaI and (TA)n microsatellite polymorphisms and woman infertility was evaluated.


METHODS
Infertile(n = 104) and fertile(n = 107) women were included in this study. We performed polymerase chain reaction-restriction fragment-length polymorphism analysis for detecting ESR1 polymorphisms.


RESULTS
PvuII and XbaI polymorphisms confered risk for infertility in a simple dominant manner in which a significant relationship was observed between infertile and control women. Infertile women had fewer(<18) short repeat alleles in promotor region. ESR1 genotypes were compared concerning maturation, fertilization, pregnancy rates and embryo quality. Although no difference was found in terms of pregnancy rates, maturation and fertilization rates were significantly smaller in pp and xx genotypes. Also, pp genotypes had significantly lower number of good quality embryos. Long TA repeat in promotor was found to be associated with low fertilization rate.


CONCLUSIONS
Polymorphisms at the ESR1 gene are associated with infertility in this Turkish infertile women population.
Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, 06500 Ankara, Turkey. ozg_e@yahoo.com
Ozge Uner
OU
Ayvaz
Abdullah
A
Ekmekçi
Volkan
V
Baltaci
Hacer Ilke
HI
Onen
Evrim
E
Unsal
D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D014421:Turkey
D047628:Estrogen Receptor alpha; C047084:DNA modification methylase PvuII; D015257:DNA-Cytosine Methylases; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific