CDK10 is not a target for aberrant DNA methylation in breast cancer.
BACKGROUND
Loss of cyclin-dependent kinase (CDK) 10 expression may be an important mechanism of tamoxifen resistance and the 5' CpG island associated with the CDK10 gene has been suggested to be a target for aberrant methylation in breast cancer.


METHODS
The methylation status of CDK10, RASSF1A (Ras association domain family 1A) and DAL-1 (differentially expressed in adenocarcinoma of the lung) was determined by means of methylation-specific PCR (MSP) in the formalin-fixed, paraffin-embedded (FFPE) surgical specimens of 96 breast carcinoma patients. Reverse transcription kinetic PCR (RT-kPCR) was used for assessment of the expression of CDK10.


RESULTS
The unmethylated form of CDK10, RASSF1A and DAL-1 was detected in all the samples analyzed. Methylation of the CDK10 5' region was not found in any of the 96 breast cancer samples. RASSF1A methylation was detected in 75 out of 96 (78%) and DAL-1 in 9 out of 15 (60%) breast cancer samples, respectively. Consistent with the methylation results, the expression of CDK10 was detected in all 96 samples.


CONCLUSIONS
CDK10 is not a target for aberrant DNA methylation in breast cancer.
Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria.
Gerwin
G
Heller
Barbara
B
Ziegler
Anita
A
Brandstetter
Sabine
S
Novak
Margaretha
M
Rudas
Guido
G
Hennig
Mathias
M
Gehrmann
Torsten
T
Acht
Sabine
S
Zöchbauer-Müller
Martin
M
Filipits
D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics*; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics*; D019175:DNA Methylation; D047628:Estrogen Receptor alpha / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
C530104:CCND1 protein, human; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D019938:Cyclin D1; C501013:CDK10 protein, human; D018844:Cyclin-Dependent Kinases