Hydration structures of 2'-deoxyguanosine studied by IR-UV double resonance spectroscopy: comparison with guanosine.
Infrared spectra of mono- and dihydrated clusters of 2'-deoxyguanosine (2'-dGs), which are formed by laser desorption combined with supersonic jet-cooling, have been measured by the technique of IR-UV double resonance spectroscopy. The structures of these hydrates are compared with those reported for guanosine (Gs) to elucidate the importance of the 2'-hydroxy group in the hydration. It is shown that monohydrated structures observed for 2'-dGs are similar to those of Gs, indicating no significant influence by the absence of 2'-OH group. For the dihydrated cluster, two structural isomers are identified and assigned to the dihydrates with the guanine moiety is in the keto form, which are consistent with the lowest-energy structures derived from theoretical calculations. Comparison with the results for Gs suggests that the presence of 2'-OH group leads to the stabilization of specific dihydrate structures involving the sugar group.
Asami
Hiroya
H
Graduate School of Arts and Sciences, Yokohama City University, Yokohama 236-0027, Japan.
Urashima
Shu-hei
SH
Saigusa
Hiroyuki
H
D003849:Deoxyguanosine / Q000737:chemistry*; D006151:Guanosine / Q000737:chemistry*; D008958:Models, Molecular; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014867:Water / Q000737:chemistry*
D014867:Water; D006151:Guanosine; D003849:Deoxyguanosine