Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen.
Cryopreservation of human semen can cause DNA damages, which compromise the fertilization and normal embryo development. The present study showed that the antioxidant resveratrol prevents these damages both in fertile and infertile men. The addition of ascorbic acid before cryopreservation can reduce DNA damages only in infertile men. Although further studies are needed, the present work showed that resveratrol could be considered in human cryopreservation procedures to avoid/minimize DNA damages and preserve sperm integrity.
Branco
Cátia S
CS
Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil.
Garcez
Márcia E
ME
Pasqualotto
Fábio F
FF
Erdtman
Bernardo
B
Salvador
Mirian
M
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D000077185:Resveratrol; D012662:Semen Preservation / Q000009:adverse effects* / Q000379:methods*; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D013267:Stilbenes; D001205:Ascorbic Acid; D000077185:Resveratrol