Equine herpesvirus type 1 quantification in different types of samples by a real-time PCR.
Equine herpesvirus type 1 (EHV-1) is one of the major viral agents causing diseases in horses common worldwide. A variety of techniques, including PCR, have been used to diagnose EHV-1 infections. In this paper, an attempt of real-time PCR has been described, which uses specific fluorochrome-labeled TaqMan probes for detection of viral DNA. This method does not require post-amplification manipulations, thereby reducing the risk of cross-contamination. The assay was sensitive enough to detect EHV-1 sequences in different clinical samples, as well in mice neuronal cell cultures. The technique was also very specific--here was no cross reaction with other human and equine herpesviruses. Compared to previously used nested PCR technique, the test was more sensitive and should be useful for the common diagnosis based on its specificity and rapidity.
Dzieciatkowski
T
T
Chair and Department of Medical Microbiology, Faculty of Medicine, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland. dzieciatkowski@wp.pl
Przybylski
M
M
Cymerys
J
J
Turowska
A
A
Chmielewska
A
A
Tucholska
A
A
Banbura
M W
MW
D000818:Animals; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D018259:Herpesvirus 1, Human; D006736:Horses; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D004279:DNA, Viral