Phenotypic inheritance induced by hairpin RNA in Drosophila.
Phenotypic inheritance induced by RNA has been documented in mouse and Caenorhabditis elegans. Here we report a similar inheritance in Drosophila. Mutant phenotypes of eye defects and antenna duplication generated from the crossing of one RNA interference (RNAi) transgenic line harboring one hairpin RNA transgene with a GAL4 driver line were inherited independently of the GAL4 driver. Hairpin RNA injection experiments demonstrated that the hairpin RNA could induce heritable mutant-like phenotypes on the eye and antenna. The penetrance of mutant phenotypes was reduced when the mutants were crossed to ago1 and piwi mutants. Our data suggest that hairpin RNA can induce phenotypic inheritance in Drosophila.
Li
Huaguang
H
CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China. hgli@sibs.ac.cn
Lu
Yi
Y
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D055029:Inverted Repeat Sequences / Q000235:genetics*; D020412:Multifactorial Inheritance / Q000235:genetics*; D010641:Phenotype; D012330:RNA, Double-Stranded / Q000235:genetics*
D012330:RNA, Double-Stranded