title: Immunostaining patterns of CD31 and podoplanin in previously untreated advanced oral/oropharyngeal cancer: prognostic implications.
abstract: BACKGROUND
  The objective of this study was to assess angiogenesis and lymphangiogenesis patterns in advanced oral/oropharyngeal cancer by immunohistochemical techniques.
  
  
  METHODS
  Forty-five patients with advanced oral/oropharyngeal cancer, treated by primary surgery between January 1996 and December 2005, were selected. All cases were followed for at least 24 months. Angiogenesis and lymphangiogenesis were evaluated with antibodies against CD31 and podoplanin, respectively. Survival outcomes were calculated by the Kaplan-Meier method, whereas univariate comparisons were obtained by log-rank, chi-square, and Mann-Whitney tests.
  
  
  RESULTS
  Survival correlated with the area of peritumoral blood vessels (p = .02), whereas the number of intratumoral lymphatics (p = .02) correlated with the occurrence of nodal metastasis. The risk for distant metastasis correlated with the perimeter of intratumoral lymphatics (p = .02).
  
  
  CONCLUSIONS
  Peritumoral angiogenesis presented different expression patterns between survivors and patients who died of disease. Intratumoral lymphangiogenesis was correlated with a higher risk of developing lymph node (LN) and distant metastasis.
authors.affiliation: Department of Otorhinolaryngology, University of Brescia, Brescia, Italy. dr.bolton@libero.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Bolzoni Villaret
authors.forename: Alberto
authors.initials: A
authors.lastname: Schreiber
authors.forename: Fabio
authors.initials: F
authors.lastname: Facchetti
authors.forename: Simona
authors.initials: S
authors.lastname: Fisogni
authors.forename: Silvia
authors.initials: S
authors.lastname: Lonardi
authors.forename: Davide
authors.initials: D
authors.lastname: Lombardi
authors.forename: Daniela
authors.initials: D
authors.lastname: Cocco
authors.forename: Luca Oscar
authors.initials: LO
authors.lastname: Redaelli de Zinis
authors.forename: Piero
authors.initials: P
authors.lastname: Nicolai
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D042583:Lymphangiogenesis / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D009959:Oropharyngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011379:Prognosis; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C117468:PDPN protein, human; D000098853:Podoplanin