DNA damage/repair and polymorphism of the hOGG1 gene in lymphocytes of AMD patients.
Oxidative stress is thought to play a role in the pathogenesis of age-related macular degeneration (AMD). We determined the extent of oxidative DNA damage and the kinetics of its removal as well as the genotypes of the Ser326Cys polymorphism of the hOGG1 gene in lymphocytes of 30 wet AMD patients and 30 controls. Oxidative DNA damage induced by hydrogen peroxide and its repair were evaluated by the comet assay and DNA repair enzymes. We observed a higher extent of endogenous oxidative DNA damage and a lower efficacy of its repair in AMD patients as compared with the controls. We did not find any correlation between the extent of DNA damage and efficacy of DNA repair with genotypes of the Ser326Cys polymorphism. The results obtained suggest that oxidative DNA damage and inefficient DNA repair can be associated with AMD and the variability of the hOOG1 gene may not contribute to this association.
Wozniak
Katarzyna
K
Department of Molecular Genetics, University of Lodz, 90-237 Lodz, Poland.
Szaflik
Jacek P
JP
Zaras
Malgorzata
M
Sklodowska
Anna
A
Janik-Papis
Katarzyna
K
Poplawski
Tomasz R
TR
Blasiak
Janusz
J
Szaflik
Jerzy
J
D016022:Case-Control Studies; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics*; D004260:DNA Repair; D005787:Gene Frequency; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D008268:Macular Degeneration / Q000097:blood / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics; D011110:Polymorphism, Genetic
D004247:DNA; D006861:Hydrogen Peroxide; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human