[Pre-treatment in determining total polysaccharide in flos Chrysanthemum indicum by response surface design].
OBJECTIVE
To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici.


METHODS
The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design.


RESULTS
The best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best.


CONCLUSIONS
A model equation that can be used to predict the experiment is established through the response surface method.
Fang
Hailing
H
Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
Guo
Qiaosheng
Q
Shen
Haijin
H
D053000:Analytic Sample Preparation Methods; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*
D010936:Plant Extracts; D011134:Polysaccharides