title: Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus.
abstract: The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12h after BmNPV infection.
authors.affiliation: Innate Immunity Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Sagisaka
authors.forename: Kosuke
authors.initials: K
authors.lastname: Fujita
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Jun
authors.initials: J
authors.lastname: Ishibashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Noda
authors.forename: Shigeo
authors.initials: S
authors.lastname: Imanishi
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
authors.forename: Minoru
authors.initials: M
authors.lastname: Yamakawa
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D017924:Nucleopolyhedroviruses / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods