Serum adipokine profile and fatty acid composition of adipose tissues are affected by conjugated linoleic acid and saturated fat diets in obese Zucker rats.
Conjugated linoleic acid (CLA) has been reported as having body fat lowering properties and the ability to modulate the inflammatory system in several models. In the present study, the effects of CLA added to saturated fat diets, from vegetable and animal origins, on the serum adipokine profile of obese Zucker rats were assessed. In addition, the fatty acid composition of epididymal and retroperitoneal adipose tissues was determined and a principal component analysis (PCA) was used to assess possible relationships between fatty acids and serum metabolites. Atherogenic diets (2 % cholesterol) were formulated with palm oil and ovine fat and supplemented or not with 1 % of a mixture (1:1) of cis-9, trans-11 and trans-10, cis-12-CLA isomers. CLA-fed animals exhibited lower daily feed intake, final body and liver weights, and hepatic lipids content. Total and LDL-cholesterol levels were increased in CLA-supplemented groups. CLA also promoted higher adiponectin and lower plasminogen activator inhibitor-1 (PAI-1) serum concentrations. In contrast to palm oil diets, ovine fat increased insulin resistance and serum levels of leptin, TNF-alpha and IL-1beta. Epididymal and retroperitoneal adipose tissues had similar deposition of individual fatty acids. The PCA analysis showed that the trans-10, cis-12-CLA isomer was highly associated with adiponectin and PAI-1 levels. Summing up, CLA added to vegetable saturated enriched diets, relative to those from animal origin, seems to improve the serum profile of adipokines and inflammatory markers in obese Zucker rats due to a more favourable fatty acid composition.
Martins
Susana V
SV
Secção de Bioquímica, CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal. smartins@fmv.utl.pt
Lopes
Paula A
PA
Alfaia
Cristina M
CM
Rodrigues
Pedro O
PO
Alves
Susana P
SP
Pinto
Rui M A
RM
Castro
Matilde F
MF
Bessa
Rui J B
RJ
Prates
José A M
JA
D054392:Adipokines / Q000097:blood*; D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D001823:Body Composition; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D004822:Epididymis; D005227:Fatty Acids / Q000032:analysis*; D050152:Intra-Abdominal Fat / Q000737:chemistry; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry; D008297:Male; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D009929:Organ Size; D000073878:Palm Oil; D010938:Plant Oils / Q000008:administration & dosage; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D051381:Rats; D011924:Rats, Zucker; D012756:Sheep
D054392:Adipokines; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D010938:Plant Oils; D017395:Plasminogen Activator Inhibitor 1; C496197:trans-10,cis-12-conjugated linoleic acid; D000073878:Palm Oil; D002784:Cholesterol