Screening of Quercus infectoria gall extracts as anti-bacterial agents against dental pathogens.
OBJECTIVE
A number of bacteria have now become antibiotic-resistant. This increases the importance of ayurvedic drugs. We report, here, the activity of different extracts (petroleum ether, chloroform, methanol and water) of Quercus infectoria galls against dental pathogens -- Streptococcus mutans, Streptococcus salivarius, Staphylococcus aureus, Lactobacillus acidophilus (designated) and Streptococcus sanguis (isolated).


METHODS
The cup-plate method was used in anti-bacterial activity of the extracts at concentration of 200 mg/ml against dental pathogens. Minimum inhibitory concentration (MIC) values of most effective extracts against the most susceptible bacteria were determined using a two-fold serial micro dilution method.


RESULTS
Methanolic extract showed maximum anti-bacterial activity against all the bacteria. The most susceptible bacteria were S. sanguis followed by S. aureus, S. mutans, S. salivarius and L. acidophilus. The MIC values showed that methanolic extract was more effective than water extract.


CONCLUSIONS
The plant has the potential to generate herbal metabolites. The crude extracts demonstrating anti-dental caries activity could result in the discovery of new chemical classes of antibiotics. These chemical classes of antibiotics could serve as selective agents for the maintenance of human health and provide bio-chemical tools for the study of infectious diseases.
Vermani
Archa
A
Department of Botany and Microbiology, Gurukul Kangri University, Haridwar, India. archa_vermani@yahoo.co.in
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D007778:Lactobacillus / Q000187:drug effects; D008515:Medicine, Ayurvedic; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010941:Plant Tumors; D029963:Quercus / Q000737:chemistry*; D013210:Staphylococcus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D010936:Plant Extracts