Identification of viral microRNAs expressed in human sacral ganglia latently infected with herpes simplex virus 2.
Deep sequencing of small RNAs isolated from human sacral ganglia latently infected with herpes simplex virus 2 (HSV-2) was used to identify HSV-2 microRNAs (miRNAs) expressed during latent infection. This effort resulted in the identification of five distinct HSV-2 miRNA species, two of which, miR-H3/miR-I and miR-H4/miR-II, have been previously reported. Three novel HSV-2 miRNAs were also identified, and two of these, miR-H7 and miR-H9, are derived from the latency-associated transcript (LAT) and are located antisense to the viral transcript encoding transactivator ICP0. A third novel HSV-2 miRNA, miR-H10, is encoded within the unique long (U(L)) region of the genome, 3' to the U(L)15 open reading frame, and is presumably excised from a novel, latent HSV-2 transcript distinct from LAT.
Umbach
Jennifer L
JL
Department of Molecular Genetics and Center for Virology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Wang
Kening
K
Tang
Shuang
S
Krause
Philip R
PR
Mont
Erik K
EK
Cohen
Jeffrey I
JI
Cullen
Bryan R
BR
D001483:Base Sequence; D002460:Cell Line; D005727:Ganglia, Spinal / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006561:Herpes Simplex / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012445:Sacrococcygeal Region / Q000821:virology*; D017735:Virus Latency
D035683:MicroRNAs; D012367:RNA, Viral