title: Plasma alkylresorcinol metabolites as potential biomarkers of whole-grain wheat and rye cereal fibre intakes in women.
abstract: It has been demonstrated that intact plasma alkylresorcinols (AR) and urinary AR metabolites could be used as biomarkers of whole-grain intake. Thereafter, we developed the method for the plasma AR metabolites, which is more convenient and requires less sample pretreatment than the analysis of intact plasma AR. The aim of the present study is to evaluate whether AR metabolites measured in plasma, in the same population, could also be considered as useful biomarkers of cereal fibre. Fifty-six women were recruited in a cross-sectional and observational study. Dietary intake (5-d record) and plasma AR metabolites (3,5-dihydroxybenzoic acid, DHBA; 3-(3,5-dihydroxyphenyl)-1-propanoic acid, DHPPA) were measured. The relationship between plasma AR metabolites and cereal fibre intake was examined using partial correlation and stepwise regression. Cereal fibre intake correlated significantly with plasma DHBA (r 0.411; P = 0.002) and DHPPA (r 0.463; P = 0.000) even after adjustment for BMI and age. Thus, plasma AR metabolites correlate with cereal fibre intake as noted with plasma intact AR and urinary AR metabolites. We observed that plasma DHPPA was the independent predictor of cereal fibre intake, explaining 18 % of the variance (adjusted r(2) 0.176; P = 0.002). In epidemiological screening, it might be easier to obtain and to collect plasma than urine samples. In addition, the plasma AR metabolites half-life seems longer than those of intact plasma AR, and their measurements are more convenient, and faster. Thus, sum of plasma AR metabolites and more specifically plasma DHPPA seems to be good and specific biomarkers of cereal fibre intake.
authors.affiliation: Institute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center, and Division of Clinical Chemistry, Biomedicum Helsinki, University of Helsinki, Haartmaninkatu 8, PO Box 63, BP 63, Room C315a, FIN-00014 Helsinki, Finland. mylene.aubertin-leheudre@helsinki.fi
authors.forename: Mylène
authors.initials: M
authors.lastname: Aubertin-Leheudre
authors.forename: Anja
authors.initials: A
authors.lastname: Koskela
authors.forename: Adile
authors.initials: A
authors.lastname: Samaletdin
authors.forename: Herman
authors.initials: H
authors.lastname: Adlercreutz
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D015992:Body Mass Index; D004043:Dietary Fiber; D002523:Edible Grain; D002149:Energy Intake; D005260:Female; D005638:Fruit; D006801:Humans; D008875:Middle Aged; D011422:Propionates / Q000097:blood / Q000652:urine; D012118:Resorcinols / Q000097:blood* / Q000652:urine; D012434:Secale; D014908:Triticum; D014675:Vegetables
chemical_list: D015415:Biomarkers; D004043:Dietary Fiber; D011422:Propionates; D012118:Resorcinols