Insights into the soluble PEG-supported synthesis of cytosine-containing nucleoside 5'-mono-, di-, and triphosphates.
Nucleoside phosphoesters (nucleotides) have crucial roles in a large variety of biological processes including nucleic acid biosynthesis and their corresponding analogues are extensively used as biological tools. Herein, we describe a new and efficient synthetic procedure involving polyethylene glycol (PEG) as soluble support and regioselective mono-, di-, and triphosphorylation steps. Applied to natural and synthetic cytosine containing nucleosides, this methodology allowed the preparation of various phosphorylated forms in high yields and good purity.
Crauste
Céline
C
UMR 5247 CNRS-UM1-UM2, Institut des Biomolécules Max Mousseron, Nucleosides and Phosphorylated Effectors Team, Université Montpellier 2, cc1705, Place E. Bataillon, 34095 Montpellier, France.
Périgaud
Christian
C
Peyrottes
Suzanne
S
D003597:Cytosine Nucleotides / Q000138:chemical synthesis*; D008722:Methods; D009711:Nucleotides / Q000138:chemical synthesis; D010766:Phosphorylation; D011092:Polyethylene Glycols / Q000737:chemistry*; D012995:Solubility
D003597:Cytosine Nucleotides; D009711:Nucleotides; D011092:Polyethylene Glycols