title: Identification and characterization of a novel member of the radical AdoMet enzyme superfamily and implications for the biosynthesis of the Hmd hydrogenase active site cofactor.
abstract: The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif defining this family, HmdB contains a unique CX(5)CX(2)C motif.
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
authors.forename: Shawn E
authors.initials: SE
authors.lastname: McGlynn
authors.forename: Eric S
authors.initials: ES
authors.lastname: Boyd
authors.forename: Eric M
authors.initials: EM
authors.lastname: Shepard
authors.forename: Rachel K
authors.initials: RK
authors.lastname: Lange
authors.forename: Robin
authors.initials: R
authors.lastname: Gerlach
authors.forename: Joan B
authors.initials: JB
authors.lastname: Broderick
authors.forename: John W
authors.initials: JW
authors.lastname: Peters
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D045858:Desulfitobacterium / Q000201:enzymology / Q000235:genetics; D006864:Hydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular
chemical_list: D001426:Bacterial Proteins; D006864:Hydrogenase