Protective effect of beta carotene pretreatment on renal ischemia/reperfusion injury in rat.
Renal ischemia/reperfusion injury is a major cause of acute renal failure. The production of free radicals and reactive oxygen species are important factors contributing to ischemia/reperfusion injury. Thus, scavenging of the excess free radicals can be an important therapeutic approach. The present study examined the protective effect of beta carotene against renal ischemia/reperfusion injury in rat. Male adult Wistar rats (250-300 g) were exposed to 45 min of renal ischemia followed by 4 h of reperfusion. Beta carotene (10, 30 and 100 mg kg(-1)) or vehicle was administered for 5 days prior to ischemia. Renal function was assessed by plasma and urinary analysis. Present results showed that ischemia/reperfusion injury increased (p < 0.05-p < 0.001) serum urea and creatinine levels, as well as urinary excretion of protein and calcium and fractional excretion of sodium, while decreased glomerular filtration rate and potassium excretion. However, alterations in these biochemical indices due to ischemia/reperfusion injury were attenuated by beta carotene pretreatment (p < 0.05-p < 0.001), although not by all doses. Since, beta carotene administration improved renal function, it seems that beta carotene protects renal tissue against ischemia/reperfusion-induced oxidative damage.
Hosseini
F
F
Physiology Research Center, Department of Physiology, School of Medicine, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran.
Naseri
M K Gharib
MK
Badavi
M
M
Ghaffari
M A
MA
Shahbazian
H
H
Rashidi
I
I
D000818:Animals; D001806:Blood Urea Nitrogen; D002118:Calcium / Q000652:urine; D003404:Creatinine / Q000097:blood; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D008297:Male; D011188:Potassium / Q000652:urine; D011507:Proteinuria / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D012964:Sodium / Q000652:urine; D019207:beta Carotene / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
D017382:Reactive Oxygen Species; D019207:beta Carotene; D012964:Sodium; D003404:Creatinine; D011188:Potassium; D002118:Calcium