title: Analysis of [U-13C6]glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry compared with two other mass spectrometry techniques.
abstract: The use of stable isotope labelled glucose provides insight into glucose metabolism. The 13C-isotopic enrichment of glucose is usually measured by gas chromatography/mass spectrometry (GC/MS) or gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). However, in both techniques the samples must be derivatized prior to analysis, which makes sample preparation more labour-intensive and increases the uncertainty of the measured isotopic composition. A novel method for the determination of isotopic enrichment of glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) has been developed. Using this technique, for which hardly any sample preparation is needed, we showed that both the enrichment and the concentration could be measured with very high precision using only 20 microL of plasma. In addition, a comparison with GC/MS and GC/IRMS showed that the best performance was achieved with the LC/IRMS method making it the method of choice for the measurement of 13C-isotopic enrichment in plasma samples.
authors.affiliation: Erasmus Medical Center-Sophia Children's Hospital, Department of Paediatrics, Division of Neonatology, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. h.schierbeek@erasmusmc.nl
authors.forename: Henk
authors.initials: H
authors.lastname: Schierbeek
authors.forename: Tanja C W
authors.initials: TC
authors.lastname: Moerdijk-Poortvliet
authors.forename: Chris H P
authors.initials: CH
authors.lastname: van den Akker
authors.forename: Frans W J
authors.initials: FW
authors.lastname: te Braake
authors.forename: Henricus T S
authors.initials: HT
authors.lastname: Boschker
authors.forename: Johannes B
authors.initials: JB
authors.lastname: van Goudoever
mesh_terms: D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D002247:Carbon Isotopes / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007223:Infant; D007700:Kinetics; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D002247:Carbon Isotopes