title: Evaluation of particulate acellular vaccines against Brucella ovis infection in rams.
abstract: The attenuated Brucella melitensis Rev 1 vaccine, used against brucellosis infection, interferes with serological diagnosis tests, may induce abortions in pregnant animals, and may infect humans. In order to overcome these drawbacks, we developed acellular vaccines based on a Brucella ovis antigenic complex (HS) containing outer membrane proteins and R-LPS entrapped in poly(anhydride) conventional and mannosylated nanoparticles (NP-HS and MAN-NP-HS) or in poly(epsilon-caprolactone) microparticles (HS-PEC) as antigen delivery systems and immunoadjuvants. Brucellosis free rams were vaccinated subcutaneously with a single dose of particles containing 3mg of HS, and challenged 6 months thereafter. Protection was evaluated by clinical, bacteriological and serological examinations, in comparison with non-vaccinated control rams. HS-PEC vaccine induced protection (7 out of 13 animals were infected) equivalent to that induced by the reference Rev 1 vaccine (8/14). In contrast, animals immunized with NP-HS were not protected, showing similar results to that obtained in the control unvaccinated rams. Furthermore HS-PEC vaccine did not interfere against B. melitensis serodiagnostic tests. In summary, HS-PEC microparticles could be used as a safe and effective vaccine against brucellosis in rams.
authors.affiliation: Immunoadjuvant unit, Department of Pharmacy and Pharmaceutical Technology and Department of Microbiology and Parasitology, University of Navarra, Pamplona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Da Costa Martins
authors.forename: Juan M
authors.initials: JM
authors.lastname: Irache
authors.forename: José M
authors.initials: JM
authors.lastname: Blasco
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Muñoz
authors.forename: Clara M
authors.initials: CM
authors.lastname: Marín
authors.forename: M
authors.initials: M
authors.lastname: Jesús Grilló
authors.forename: M
authors.initials: M
authors.lastname: Jesús De Miguel
authors.forename: Montserrat
authors.initials: M
authors.lastname: Barberán
authors.forename: Carlos
authors.initials: C
authors.lastname: Gamazo
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000276:immunology*; D042061:Brucella ovis / Q000276:immunology*; D002006:Brucellosis / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D004337:Drug Carriers; D005260:Female; D007279:Injections, Subcutaneous; D008081:Liposomes / Q000494:pharmacology; D053758:Nanoparticles; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D002004:Brucella Vaccine; D004337:Drug Carriers; D008081:Liposomes; D022282:Vaccines, Acellular