Slow-freezing cryopreservation of neural stem cell spheres with different diameters.
Neural stem cells (NSCs) are of great value for clinical application and scientific research. The development of efficient cryopreservation protocols could significantly facilitate the storage and transportation for clinic applications. The objective of the present study is to improve the survival rate and viability of NSCs. Neural stem cells with three states of single-cell suspension, NSC spheres with diameters of 30-50 microm and 80-100 microm, were cryopreserved by slow-freezing method with the cryoprotective agent (CPA) of dimethyl sulfoxide (Me(2)SO), respectively. Then the post-thawing NSCs were tested for the survival rate and the differentiation ability. As a result, NSC spheres with diameter of 80-100 microm and Me(2)SO concentration of 8% achieve the survival rate of 82.9%, and the NSCs still sustain the multi-differentiation potentiality. These results indicated that both the subtle interaction among NSCs and sphere diameters may affect the survival rate together.
Ma
Xue-Hu
XH
Dalian R&D Center for Stem Cell and Tissue Engineering, Department of Chemical Engineering, Dalian University of Technology, Dalian, China. xuehuma@dlut.edu.cn
Shi
Ying
Y
Hou
Ying
Y
Liu
Yang
Y
Zhang
Lei
L
Fan
Wen-Xia
WX
Ge
Dan
D
Liu
Tian-Qing
TQ
Cui
Zhan-Feng
ZF
D000818:Animals; D001562:Benzimidazoles; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D048429:Cell Size; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D053595:Embryonic Stem Cells / Q000166:cytology; D005456:Fluorescent Dyes; D009474:Neurons / Q000166:cytology; D011419:Propidium; D051381:Rats; D013997:Time Factors
D001562:Benzimidazoles; D003451:Cryoprotective Agents; D005456:Fluorescent Dyes; D011419:Propidium; C017807:bisbenzimide ethoxide trihydrochloride; D004121:Dimethyl Sulfoxide