title: Accidental infection by Trypanosoma cruzi follow-up by the polymerase chain reaction: case report.
abstract: We report a case of accidental infection by Trypanosoma cruzi in a 42-year-old female patient who presented an inoculation chagoma. Laboratory confirmation was based on examination of fresh blood, Giemsa-stained blood smear, immunoenzyme test (EIA-IgG), indirect immunofluorescence (IIF-IgM, IgG) and polymerase chain reaction (PCR). Only the PCR gave a positive result, and the EIA test was inconclusive. Two treatments with benznidazole were necessary. PCR was the only technique that continued to give positive results for approximately two months (65 days, or 2.2 months) following the second treatment and negative results from 96 days (3.2 months) to 850 days (28.3 months). We concluded that the presence of an inoculation chagoma and use of PCR were important and decisive for diagnosis and follow-up of the case.
authors.affiliation: Departamento de Análises Clínicas, Laboratório de Doença de Chagas, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: Andréa Tieko
authors.initials: AT
authors.lastname: Kinoshita-Yanaga
authors.forename: Max Jean de Ornelas
authors.initials: MJ
authors.lastname: Toledo
authors.forename: Silvana Marques de
authors.initials: SM
authors.lastname: Araújo
authors.forename: Berenice Pelizza
authors.initials: BP
authors.lastname: Vier
authors.forename: Mônica Lúcia
authors.initials: ML
authors.lastname: Gomes
mesh_terms: D000062:Accidents, Occupational; D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology* / Q000635:transmission*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006230:Hand Injuries / Q000469:parasitology; D006801:Humans; D017056:Medical Laboratory Personnel; D009593:Nitroimidazoles / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D014344:Trypanocidal Agents / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology; D014951:Wounds, Stab / Q000469:parasitology
chemical_list: D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole