Maturation of 23S rRNA in Bacillus subtilis in the absence of Mini-III.
23S rRNA maturation in Bacillus subtilis is catalyzed by the recently characterized enzyme Mini-RNase-III. Mini-III is dispensable, however, and 23S rRNA is matured by other ribonucleases in strains lacking this enzyme. Here we show that these RNases are the 5'-to-3' exoribonuclease RNase J1 and the 3'-to-5' exoribonucleases, principally RNase PH and YhaM.
Redko
Yulia
Y
Institut de Biologie Physico-Chimique, CNRS UPR 9073, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Condon
Ciarán
C
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012338:RNA, Ribosomal, 23S / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D012338:RNA, Ribosomal, 23S; C056502:ribonuclease PH; D005095:Exoribonucleases; D043244:Ribonuclease III