Ecophysiological analysis of microorganisms in complex microbial systems by combination of fluorescence in situ hybridization with extracellular staining techniques.
Ecophysiological analysis and functions of single cells in complex microbial systems can be examined by simple combinations of Fluorescence in situ hybridization (FISH) for identification with various staining techniques targeting functional phenotypes. In this chapter, we describe methods and protocols optimized for the study of extracellular enzymes, surface hydrophobicity and specific surface structures. Although primarily applied to the study of microbes in wastewater treatment (activated sludge and biofilms), the methods may also be used with minor modifications in several other ecosystems.
Nielsen
Jeppe Lund
JL
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
Kragelund
Caroline
C
Nielsen
Per Halkjaer
PH
D018441:Biofilms / Q000254:growth & development; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012722:Sewage / Q000382:microbiology; D014871:Water Microbiology
D012722:Sewage