[Expression CD27 on T and B lymphocytes in hypertrophied adenoids at children with otitis media with effusion].
BACKGROUND
Adenoid has particular meaning to develop of immunological response to inflammations in upper respiratory inclusive middle ear. The mining of antigen CD27 on lymphocytes T and B in creation of memory cells is still unclear.


OBJECTIVE
CD27 on lymphocytes T and B has a crucial role in development of immune response against inflammatory state. Aim of this study was evaluation functions of lymhocytes with expression CD27 in hypertrophied adenoid in children with otitis media with effusion.


METHODS
[corrected] We tested 24 children in examine group with hypertrophied adenoid and otitis media with effusion (OME), and 29 children in comparative group only with hypertrophied adenoid (HA). We also divided both groups into two groups, above 5 and over 5 years old. We made the research by flow cytometry method. We used anty-CD19, anty-CD3, anty-CD5 and anty-CD27 monoclonal antibodies to examinations.


RESULTS
We showed the lowest percentage lymphocytes B CD5+ with expression of CD27 in subgroup older children with otitis media with effusion (O.M.E. 22.43 +/- 2.66%) in compartative to younger children in the same group (15.96 +/- 5.31%; p < 0.005) and to older comparative subgroup (H.A. 15.50 +/- 8.03; p < 0.001). Percentages of B CD5 cells with expression of CD27 was statistical lower in examinated group (7.25 +/- 3.81) than in comparative group (H.A. 16.26 +/- 5.82; p < 0.04). In group with hypertrophied adenoid showed higher percentages of CD5-CD27+CD19+ cells both in younger and older supgroup than in equivalent supgroups with O.M.E.


CONCLUSIONS
Iteraction of CD27-CD70 regulates many functions of T and B lymphocytes. It seems that disorders humoral immunologucal answer in hypertrophied adenoids are supported to develop of otitis media with effusion.
Ratomski
Karol
K
Zakład Laboratoryjnej Diagnostyki Pediatrycznej Uniwersytetu Medycznego w Białymstoku. karol.cam@interia.pl
Zelazowska-Rutkowska
Beata
B
Wysocka
Jolanta
J
Skotnicka
Bozena
B
Kasprzycka
Edwina
E
Hassmann-Poznańska
Elzbieta
E
D000234:Adenoids / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes; D018956:CD5 Antigens / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006984:Hypertrophy / Q000276:immunology; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000276:immunology*; D014068:Tonsillectomy; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism*
D018941:Antigens, CD19; D015415:Biomarkers; D017252:CD3 Complex; D018956:CD5 Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7