Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation.
Rats were intraperitoneally treated once with compound 48/80 (C48/80), a mast cell degranulator, (0.75 mg/kg). Serum serotonin, histamine and corticosterone levels increased 0.5 h after C48/80 treatment, but their increases were reduced thereafter. Adrenal total ascorbic acid (ascorbic acid plus dehydroascorbic acid), ascorbic acid and dehydroascorbic acid levels decreased 0.5, 3 or 6 h after C48/80 treatment, adrenal lipid peroxide level increased at 3 and 6 h, adrenal non-protein-SH level decreased at 3 and 6 h and adrenal beta-tocopherol level decreased at 3 h. Ketotifen, a mast cell stabilizer (1 mg/kg) administered intraperitoneally at 0.5 h before C48/80 treatment, attenuated all these changes found in the serum and adrenal at 3 h after treatment, while beta-tocopherol (250 mg/kg), administered orally at 0.5 h after C48/80 treatment, attenuated all these changes in the adrenal tissue. These results indicate that C48/80 causes oxidative stress in rat adrenal gland through mast cell degranulation.
Kaida
Shingo
S
Department of Anesthesiology, 2nd Teaching Hospital, Fujita Health University School of Medicine, Nagoya, Aichi 454-0012, Japan.
Ohta
Yoshiji
Y
Imai
Yoichiro
Y
Ohashi
Koji
K
Kawanishi
Minoru
M
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D003345:Corticosterone / Q000097:blood; D006632:Histamine / Q000097:blood; D007274:Injections, Intraperitoneal; D007665:Ketotifen / Q000008:administration & dosage; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000097:blood; D024502:alpha-Tocopherol / Q000008:administration & dosage; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D024502:alpha-Tocopherol; D003345:Corticosterone; D007665:Ketotifen