title: Dynamic NAD(P)H post-synaptic autofluorescence signals for the assessment of mitochondrial function in a neurodegenerative disease: monitoring the primary motor cortex of G93A mice, an amyotrophic lateral sclerosis model.
abstract: Abnormal mitochondrial function was reported in patients and models for amyotrophic lateral sclerosis (ALS). It is therefore important to set up sensitive tools for the monitoring of active agents that enhance energy metabolism delay onset, and extend lifespan of transgenic G93A-SOD1 ALS mice. In this report, primary motor cortex slices from G93A mice at different stages of disease were studied, using NAD(P)H autofluorescence post-synaptic signals following ultraviolet stimuli, as a probe to evaluate mitochondrial function. We observed consistent age-related alterations of responses in G93A primary motor cortex slices versus controls. We conclude that NAD(P)H autofluorescence post-synaptic signal is a highly sensitive real-time technique to detect mitochondrial function failure in primary cortex from living tissues.
authors.affiliation: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy. stefanoloiz@libero.it <stefanoloiz@libero.it>
authors.forename: Stefano
authors.initials: S
authors.lastname: Loizzo
authors.forename: Massimo
authors.initials: M
authors.lastname: Pieri
authors.forename: Alberto
authors.initials: A
authors.lastname: Ferri
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Carrì
authors.forename: Cristina
authors.initials: C
authors.lastname: Zona
authors.forename: Andrea
authors.initials: A
authors.lastname: Fortuna
authors.forename: Stefano
authors.initials: S
authors.lastname: Vella
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology*; D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism*; D009044:Motor Cortex / Q000473:pathology*; D009249:NADP / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D009249:NADP