title: Applications of genome-scale metabolic reconstructions.
abstract: The availability and utility of genome-scale metabolic reconstructions have exploded since the first genome-scale reconstruction was published a decade ago. Reconstructions have now been built for a wide variety of organisms, and have been used toward five major ends: (1) contextualization of high-throughput data, (2) guidance of metabolic engineering, (3) directing hypothesis-driven discovery, (4) interrogation of multi-species relationships, and (5) network property discovery. In this review, we examine the many uses and future directions of genome-scale metabolic reconstructions, and we highlight trends and opportunities in the field that will make the greatest impact on many fields of biology.
authors.affiliation: Department of Biomedical Engineering, University of Virginia, Health System, Charlottesville, VA, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Oberhardt
authors.forename: Bernhard Ø
authors.initials: BØ
authors.lastname: Palsson
authors.forename: Jason A
authors.initials: JA
authors.lastname: Papin
mesh_terms: D005075:Biological Evolution; D005818:Genetic Engineering; D016678:Genome / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008957:Models, Genetic