Relationship between NFKB1 -94 insertion/deletion ATTG polymorphism and susceptibility of cervical squamous cell carcinoma risk.
BACKGROUND
A very high expression of nuclear factor-kappa B protein (nuclear p50, encoded by NFKB1) in high-grade squamous intraepithelial lesion and invasive cancers has been observed. The aim of this study was to determine whether the functional NFKB1 -94 insertion/deletion ATTG polymorphism (rs28362491) is associated with cervical squamous cell carcinoma (CSCC).


METHODS
PCR-polyacrylamide gel electrophoresis method was used to genotype the NFKB1 -94 insertion/deletion ATTG polymorphism in 233 women with CSCC and 365 ethnicity-matched healthy control women. The genotyping method was confirmed by the DNA sequencing analysis.


RESULTS
The frequency of ATTG(2)/ATTG(2) genotype and ATTG(2) allele in the CSCC patients was significantly higher than that of controls, indicating that the -94 insertion/deletion ATTG polymorphism in NFKB1 promoter was associated with CSCC [P = 0.001, odds ratio (OR) = 2.560, 95% confidence interval (CI) 1.459-4.492 and P = 0.001, OR = 1.493, 95% CI 1.168-1.908, respectively]. Results of stratified analyses revealed that this polymorphism is associated with younger age (< or =35 years) and positive parametrial invasion but not with tumor differentiation, high clinical stage or lymph node status.


CONCLUSIONS
Our results indicate that the functional NFKB1 -94 insertion/deletion ATTG polymorphism is associated with CSCC, especially with younger age (< or =35 years) and positive parametrial invasion of CSCC patients.
Zhou
B
B
Laboratory of Molecular Translational Medicine.
Qie
M
M
Department of Obstetrics and Gynecology, West China Second University Hospital.
Wang
Y
Y
Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
Yan
L
L
Department of Obstetrics and Gynecology, West China Second University Hospital.
Zhang
Z
Z
Department of Obstetrics and Gynecology, West China Second University Hospital.
Liang
A
A
Department of Obstetrics and Gynecology, West China Second University Hospital.
Wang
T
T
Laboratory of Molecular Translational Medicine.
Wang
X
X
Laboratory of Molecular Translational Medicine.
Song
Y
Y
Laboratory of Molecular Translational Medicine.
Zhang
L
L
Laboratory of Molecular Translational Medicine. Electronic address: zhanglin@scu.edu.cn.
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human