Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens.
In the present study, biosynthesis of silver nanoparticles and its activity on water borne bacterial pathogens were investigated. Silver nanoparticles were rapidly synthesized using leaf extract of Acalypha indica and the formation of nanoparticles was observed within 30min. The results recorded from UV-vis spectrum, scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS) support the biosynthesis and characterization of silver nanoparticles. From high-resolution transmission electron microscopy (HRTEM) analysis, the size of the silver nanoparticles was measured 20-30nm. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against water borne pathogens Viz., Escherichia coli and Vibrio cholerae. Silver nanoparticles 10microg/ml were recorded as the minimal inhibitory concentration (MIC) against E. coli and V. cholerae. Alteration in membrane permeability and respiration of the silver nanoparticle treated bacterial cells were evident from the activity of silver nanoparticles.
Krishnaraj
C
C
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai, India. krishnarajbio@gmail.com
Jagan
E G
EG
Rajasekar
S
S
Selvakumar
P
P
Kalaichelvan
P T
PT
Mohan
N
N
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D019665:Euphorbiaceae / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D014734:Vibrio cholerae / Q000187:drug effects; D014871:Water Microbiology; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver