title: Usefulness of addition of Orvus ES paste and sodium lauryl sulfate to frozen feline semen.
abstract: It has been shown that addition of the surfactant Orvus ES paste (OEP) and its main component sodium lauryl sulfate (SLS) to boar or dog semen before freezing improves post-thaw sperm motility and protects acrosome caps. In this study, we investigated the usefulness of the addition of OEP (0, 1, 2 and 4%) or SLS (0, 1, 2, 3 and 4 mg/ml) to cat ejaculates before freezing and their concentrations. Among the OEP addition groups, the 1% OEP group showed higher sperm motility than the other groups. Among the SLS addition groups, the 3 mg/ml SLS group showed slightly higher sperm motility and viability than the other groups. Comparison between the 1% OEP and 3 mg/ml SLS addition groups suggested a higher percentage of sperm with an acrosome cap in the 1% OEP group. The other sperm properties did not significantly differ between the 2 groups. These results indicate that addition of 1% OEP or 3 mg/ml SLS is effective for freezing of cat ejaculated semen.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan. tatsuji@mizutani-ah.com
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Mizutani
authors.forename: Shiho
authors.initials: S
authors.lastname: Sumigama
authors.forename: Keiichi
authors.initials: K
authors.lastname: Nagakubo
authors.forename: Noriko
authors.initials: N
authors.lastname: Shimizu
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Oba
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D002415:Cats; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate