[Effect of Santalum album on tissue distribution of danshensu in rabbits by HPLC].
OBJECTIVE
To investigate the influence of Shi herb (Santalum album, SA) to the tissue distribution of danshensu (DSS) which is the main hydrosoluble component of Jun herb (Salvia miltiorrhiza, SM) in rabbits by HPLC.


METHODS
Rabbits were oral administrated decoction of SM and SM-SA, respectively. Perchloric acid (10%) was used to precipitate the tissue samples of rabbits heart, brain, liver, kidney, acetic ether was used to extracte supernatant, and the internal standard was p-hydroxybenzoic acid. The content of DSS of SM in tissues was detected.


RESULTS
The content of DSS reached the highest point close to 50 min in the mentioned tissues. Before and after co-administration, the sequences of average concentration of DSS in tissues were C(kidney) > C(heart) > C(brain) > C(liver) and C(kidney) > C(liver) > C(brain) > C(heart) respectively. Compared with SM administrated singly, the content of DSS in every tissues of co-administration was higher.


CONCLUSIONS
In Danshenyin Formulae, SA can increase concentration of DSS in target tissues significantly, and therefore therapeutic effect of SM for cardiovascular disease is raised.
Zhang
Qian
Q
College of Life Science, Northwest University, Xi'an 710069, China.
Tang
Yimei
Y
Wang
Rui
R
Wang
Shixiang
S
Fang
Minfeng
M
Zhang
Yajun
Y
Zheng
Xiaohui
X
D000818:Animals; D001921:Brain / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D007773:Lactates / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D008099:Liver / Q000187:drug effects; D011817:Rabbits; D032123:Santalum / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid