Molecular cloning, functional analysis and localization of a novel gene encoding polygalacturonase-inhibiting protein in Chorispora bungeana.
Polygalacturonase-inhibiting proteins (PGIPs) are plant defense proteins. To date, no spatial distribution of PGIPs and interaction between PGIPs and nitric oxide (NO) in plant were described. Here, we first reported the full-length cDNA sequence of PGIP of Chorispora bungeana (CbPGIP1). Notably, immunofluorescence localization showed that the CbPGIP was evenly distributed in leaves but it was mainly localized in epidermis and vascular bundle in stems and roots. Further studies indicated that CbPGIP had higher abundance in roots than in stems and leaves. Conversely, the bulk PGIP of C. bungeana showed a higher activity in leaves than in stems and roots. In addition, quantitative real-time polymerase chain reaction demonstrated that CbPGIP1 expression was induced by Stemphylium solani, salicylic acid (SA), 4, -4 degrees C and NO. This is a first report attempting to predict if NO can induce the PGIP expression. Taken together, these findings showed that the gene was spatially regulated and NO and SA might take part in CbPGIP1 expression induced by biotic and abiotic stresses. This study highlighted the potential importance of CbPGIP1 and NO in plant resistance.
Di
Cuixia
C
Key Laboratory of Arid and Grassland Agroecology (Ministry Education), School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China.
Li
Ming
M
Long
Feng
F
Bai
Muqun
M
Liu
Yajie
Y
Zheng
Xiaolin
X
Xu
Shijian
S
Xiang
Yun
Y
Sun
Zhenglong
Z
An
Lizhe
L
D000595:Amino Acid Sequence; D019607:Brassicaceae / Q000187:drug effects / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D005455:Fluorescent Antibody Technique; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000494:pharmacology; D010802:Phylogeny; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D020156:Salicylic Acid / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
C081479:PGIP protein, plant; D010940:Plant Proteins; D009569:Nitric Oxide; D020156:Salicylic Acid