Evaluation of sulfobetaine-type polymer resin as an SPE adsorbent in the analysis of trace tetracycline antibiotics in honey.
A new sulfobetaine polymer resin SPE method combined with HPLC-MS/MS for the determination of tetracycline (TC) antibiotics residues from honey samples is presented. The sulfobetaine resin was synthesized and was packed into a syringe-type tube, which served as the SPE cartridge for selective adsorption of TCs. TCs were quantitatively adsorbed on the sulfobetaine cartridge, when the loading solvent was 95% v/v acetonitrile solution, and TCs adsorbed were not eluted by aqueous acetonitrile washing solution. TFA aqueous solution was used for eluting the adsorbed TCs. The proposed SPE method has been applied to the determination of TCs in honey samples. The recoveries of TCs spiked in honey samples ranged from 70 to 80%. Reduction of the recoveries might be derived from low solubility of TCs in acetonitrile. Compared with other SPE resins, this resin was superior in terms of selectivity with simple pretreatment.
Tsukamoto
Tomoyasu
T
College of Bioscience and Biotechnology, Chubu University, Kasugai-shi, Aichi, Japan. gr07804-7682@sti.chubu.ac.jp
Yasuma
Mari
M
Yamamoto
Atsushi
A
Hirayama
Kayo
K
Kihou
Takafumi
T
Kodama
Shuji
S
Inoue
Yoshinori
Y
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D002853:Chromatography, Liquid; D006722:Honey / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry; D013752:Tetracycline / Q000032:analysis*
D000900:Anti-Bacterial Agents; D011108:Polymers; D001622:Betaine; C483727:sulfobetaine; D013752:Tetracycline