title: OPTIMIZATION OF A MICROFLUIDIC DEVICE FOR DIFFUSION-BASED EXTRACTION OF DMSO FROM A CELL SUSPENSION.
abstract: This study considers the use of a two-stream microfluidic device for extraction of dimethyl sulphoxide (DMSO) from a cryopreserved cell suspension. The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel. The model of Fleming et al.[14] is employed to determine and discuss optimal geometry and operating conditions for a case requiring removal of 95% DMSO from suspension streams with volumetric flow rates up to 2.5 ml/min. The effects of Peclet number, flow rate fraction, and cell volume fraction are analyzed, and expansion of the analysis to other applications is discussed.
authors.affiliation: Department of Mechanical Engineering, 1100 Mechanical Engineering, 111 Church Street, University of Minnesota, Minneapolis, MN 55455.
authors.forename: K K
authors.initials: KK
authors.lastname: Fleming Glass
authors.forename: E K
authors.initials: EK
authors.lastname: Longmire
authors.forename: A
authors.initials: A
authors.lastname: Hubel