A novel lipidomic strategy reveals plasma phospholipid signatures associated with respiratory disease severity in cystic fibrosis patients.
The aim of this study was to search for lipid signatures in blood plasma from cystic fibrosis (CF) patients using a novel MALDI-TOF-ClinProTools strategy, initially developed for protein analysis, and thin layer chromatography coupled to MALDI-TOF (TLC-MALDI). Samples from 33 CF patients and 18 healthy children were subjected to organic extraction and column chromatography separation of lipid classes. Extracts were analyzed by MALDI-TOF, ion signatures were compared by the ClinProTools software and by parallel statistical analyses. Relevant peaks were identified by LC-MSn. The ensemble of analyses provided 11 and 4 peaks differentially displayed in CF vs healthy and in mild vs severe patients respectively. Ten ions were significantly decreased in all patients, corresponding to 4 lysophosphatidylcholine (18:0, 18:2, 20:3, and 20:5) and 6 phosphatidylcholine (36:5, O-38:0, 38:4, 38:5, 38:6, and P-40:1) species. One sphingolipid, SM(d18:0), was significantly increased in all patients. Four PC forms (36:3, 36:5, 38:5, and 38:6) were consistently downregulated in severe vs mild patients. These observations were confirmed by TLC-MALDI. These results suggest that plasma phospholipid signatures may be able to discriminate mild and severe forms of CF, and show for the first time MALDI-TOF-ClinProTools as a suitable methodology for the search of lipid markers in CF.
Guerrera
Ida Chiara
IC
Plateau Proteome Necker, Université Paris Descartes, IFR94, Paris, France.
Astarita
Giuseppe
G
Jais
Jean-Philippe
JP
Sands
Dorota
D
Nowakowska
Anna
A
Colas
Julien
J
Sermet-Gaudelus
Isabelle
I
Schuerenberg
Martin
M
Piomelli
Daniele
D
Edelman
Aleksander
A
Ollero
Mario
M
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002855:Chromatography, Thin Layer / Q000379:methods; D003550:Cystic Fibrosis / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007223:Infant; D008055:Lipids / Q000737:chemistry*; D008244:Lysophosphatidylcholines / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010743:Phospholipids / Q000097:blood*; D012120:Respiration Disorders / Q000097:blood* / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008055:Lipids; D008244:Lysophosphatidylcholines; D010743:Phospholipids