Molecular cloning and expression analysis of ovary-specific transcript 1 (Pm-OSTI) of the giant tiger shrimp, Penaeus monodon.
Isolation and characterization of genes specifically expressed in ovaries are necessary for understanding sex differentiation and ovarian development processes in the giant tiger shrimp, Penaeus monodon. In this study, a transcript that significantly matched the polehole precursor was further characterized by RACE-PCR. The sequence obtained was 5151 bp in length and contained a coding region of 5031 bp corresponding to 1677 amino acids. This transcript was only expressed in ovaries but not in testes of Juveniles (N = 10) and broodstock (N = 22) of P. monodon. A tissue distribution analysis further confirmed ovary-specific expression of this transcript (called P. monodon ovary-specific transcript 1, Pm-OST1) in female broodstock. Expression levels of Pm-OST 1 in ovaries of juvenile P. monodon upon 5-HT Injection (33.9+/-6.40 g; 50 microg/g body weight) were significantly higher at 12-72 hours post Injection (P<0.05). Quantitative real-time PCR Indicated that Pm-OST1 was comparably expressed throughout ovarian development in normal P. monodon broodstock (P>0.05). However, the expression level of Pm-OST1 was significantly higher in stage-III ovaries in eyestalk-ablated broodstock (P<0.05). Pm-OST1 was clearly localized in the ooplasm of previtellogenic and vitellogenic oocytes. Our results suggest that Pm-OST1 plays a functionally Important role in promoting the development of female germ cells and oocytes in P. monodon.
Klinbunga
Sirawut
S
Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong Luang, Pathumthani 12120, Thailand. sirawut@biotec.or.th
Sittikankaew
Kanchana
K
Yuvanatemiya
Vasin
V
Preechaphol
Rachanimuk
R
Prasertlux
Sirikan
S
Yamano
Keisuke
K
Menasveta
Piamsak
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D015854:Up-Regulation
D011506:Proteins; D012701:Serotonin