Super-resolution optical microscopy: multiple choices.
The recent invention of super-resolution optical microscopy enables the visualization of fine features in biological samples with unprecedented clarity. It creates numerous opportunities in biology because vast amount of previously obscured subcellular processes now can be directly observed. Rapid development in this field in the past two years offers many imaging modalities that address different needs but they also complicates the choice of the 'perfect' method for answering a specific question. Here I will briefly describe the principles of super-resolution optical microscopy techniques and then focus on comparing their characteristics in various aspects of practical applications.
Huang
Bo
B
Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA. bhuang@picasso.ucsf.edu
D007091:Image Processing, Computer-Assisted; D008027:Light; D008853:Microscopy / Q000379:methods; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D055095:Optics and Photonics