Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus.
The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12h after BmNPV infection.
Sagisaka
Aki
A
Innate Immunity Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
Fujita
Kosuke
K
Nakamura
Yuki
Y
Ishibashi
Jun
J
Noda
Hiroaki
H
Imanishi
Shigeo
S
Mita
Kazuei
K
Yamakawa
Minoru
M
Tanaka
Hiromitsu
H
D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D017924:Nucleopolyhedroviruses / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods