[Determination of anti-oxidative activity of different fractions of Xinjiang red raspberry fruits extract].
OBJECTIVE
To determination total phenolics content of raspberry different polar solvent extracts was measured by Folin-Ciocalteu method.


METHODS
Red raspberry fruits were extracted with 95% ethanol, defatted with petroleum ether, and then partitioned with petroleum ether, ethyl acetate and butanol successively. Total phenolic content of each part was measured by Folin-Ciocalteu method and calculated as gallic acid equivalents (GAE). Stability of the method were assayed with RSD (relative standard deviation). Antioxidant activities of the different extractions were evaluated with DPPH* and OH* scavenging assay.


RESULTS
(1) The method had high quality of precision, stability, repetition rate. (2) Total phenolic contents were varied from GAE 10.56 mg/g to GAE 96.76 mg/g as gallic acid equivalent, and ethyl acetate extracts had the highest total phenolic content (GAE 96.76 mg/g). (3) The crude extracts and different soluble fractions showed good antioxidative activities.


CONCLUSIONS
Raspberry could be good resource of natural antioxidant.
Han
Jia
J
College of Public Health, Xinjiang Medical University, Urumqi 830054, China.
Hai
Liqian
L
Chen
Peipei
P
Mirban
Shabor
S
Nurdana
Liu
Jiwen
J
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027824:Rosaceae / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid