The oxidation status of ALDH3A1 in human saliva and its correlation with antioxidant capacity measured by ORAC method.
Oxidation status of the salivary aldehyde dehydrogenase (ALDH) was measured in healthy human population using two-assay fluorimetric method and compared with antioxidant capacity (ORAC) in non-smoking and heavy smokers group. Influence of high or low antioxidant diet was also examined. Except for the group of smokers, the salivary ALDH oxidation degree in human saliva was not correlated with antioxidant capacity. Simultaneously direct administration of the antioxidant-containing drug, Fluimucil, resulted in short-term, but statistically significant increase of the reduced (active) form of the enzyme, presumably due to a radical-scavenging activity of the drug.
Bogucka
Małgorzata
M
Department of Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., PL-02097 Warsaw, Poland.
Giebułtowicz
Joanna
J
Zawada
Katarzyna
K
Wroczyński
Piotr
P
Wierzchowski
Jacek
J
Pietrzak
Monika
M
Piekarczyk
Piotr
P
Romanowska
Katarzyna
K
D000111:Acetylcysteine / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D004032:Diet; D005260:Female; D005470:Fluorometry / Q000379:methods; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012463:Saliva / Q000201:enzymology* / Q000378:metabolism; D012907:Smoking / Q000378:metabolism*; D055815:Young Adult
D000975:Antioxidants; D016166:Free Radical Scavengers; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase; D000111:Acetylcysteine