Markers of haemostasis and angiogenesis in placentae from gestational vascular complications: impairment of mechanisms involved in maintaining intervillous blood flow.
BACKGROUND
Preeclampsia (PE) and fetal growth restriction (FGR) are multifactorial diseases, whose pathogenesis is largely unknown. A significant relationship between haemostasis and angiogenesis in placentae from uneventful pregnancies was previously shown.


METHODS
RNA expression of haemostasis (TF, TFPI, TFPI-2, PAI-2, Anx V, TM) and angiogenesis (Ang-1, Ang-2, PlGF, VEGF) markers in placentae from PE (n=12), PE+FGR (n=17) and FGR (n=20) in respect of placentae from uncomplicated pregnancies (n=21) were investigated.


RESULTS
Placentae from complicated pregnancies showed a significant lower expression (p<or=0.05 Mann-Whitney U test) of TF, TFPI, TFPI-2, Anx V, PAI-2 than those from in uncomplicated ones. VEGF and PlGF were not different in the considered groups; Ang-1 and Ang-2 were significantly higher (p<or=0.05 Mann-Whitney U test) in the PE group. Correlations between factors involved in haemostasis and those involved in angiogenesis, observed in placentae from uneventful pregnancies are lacking in those from complicated ones.


CONCLUSIONS
Haemostasis factors are reduced in placentae from complicated pregnancies. The relationship between haemostasis and angiogenesis observed in uncomplicated pregnancies is impaired in PE and FGR.
Chinni
Elena
E
Atherosclerosis and Thrombosis Unit, I.R.C.C.S. Casa Sollievo della Sofferenza, S.Giovanni Rotondo, Foggia, Italy.
Colaizzo
Donatella
D
Tiscia
Giovanni L
GL
Martinelli
Pasquale
P
Maruotti
Giuseppe M
GM
Matteo
Maria
M
Margaglione
Maurizio
M
Grandone
Elvira
E
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000098:blood supply; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010920:Placenta / Q000098:blood supply* / Q000166:cytology / Q000502:physiology*; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000378:metabolism*; D012039:Regional Blood Flow
D015415:Biomarkers