title: Chelex-based DNA isolation procedure for the identification of microbial communities of eggshell surfaces.
abstract: Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total communities of eggshell surfaces from six avian species.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
authors.forename: Antonio Manuel
authors.initials: AM
authors.lastname: Martín-Platero
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Peralta-Sánchez
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Soler
authors.forename: Manuel
authors.initials: M
authors.lastname: Martínez-Bueno
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001717:Birds / Q000196:embryology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000302:isolation & purification; D004528:Egg Shell / Q000382:microbiology*; D004625:Embryo, Nonmammalian / Q000196:embryology; D046109:Passeriformes / Q000382:microbiology; D011137:Polystyrenes; D011145:Polyvinyls; D020305:Strigiformes / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex