Overexpression of CsNMAPK in tobacco enhanced seed germination under salt and osmotic stresses.
In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt and osmotic stresses, transgenic tobacco plants constitutively overexpressing CsNMAPK were generated. Northern and Western blot analysis showed that strong signals were detected in the RNA and protein samples extracted from transgenic lines, whereas no signal was detected in the wild type tobacco, indicating that CsNMAPK was successfully transferred into tobacco genome and overexpressed. The results of seed germination showed that germination rates of transgenic lines were significantly higher than wild type under high salt and osmotic stresses. In addition, seed growth of transgenic lines was much better than wild type under salt and osmotic stresses. These results indicated that overexpression of CsNMAPK positively regulated plant tolerance to salt and osmotic stresses.
Xu
Huini
H
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
Li
Kunzhi
K
Yang
Fengjuan
F
Shi
Qinghua
Q
Wang
Xiufeng
X
D015152:Blotting, Northern; D015153:Blotting, Western; D028462:Cucumis / Q000201:enzymology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects*; D008353:Mannitol / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009995:Osmosis / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D012639:Seeds / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D008353:Mannitol; D012965:Sodium Chloride; D020928:Mitogen-Activated Protein Kinases