title: Synthesis of a mitochondria-targeted spin trap using a novel Parham-type cyclization.
abstract: A new cyclic nitrone spin trap, [4-(3',3'-dibutyl-2'-oxy-3'H-isoindol-5'-yloxy)butyl]triphenylphosphonium bromide (MitoSpin), bearing a lipophilic cation has been prepared by a route that involves a novel Parham-type lithiation-cyclization of an isocyanate to give the isoindolinone core. MitoSpin accumulates in a membrane potential dependent way in energized mitochondria and its oxidation could potentially be used in the study of oxidative stress resulting from reactive oxygen species generated in mitochondria.
authors.affiliation: Centre for the Chemical Research of Ageing, WestCHEM Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, UK.
authors.forename: Caroline
authors.initials: C
authors.lastname: Quin
authors.forename: Jan
authors.initials: J
authors.lastname: Trnka
authors.forename: Alison
authors.initials: A
authors.lastname: Hay
authors.forename: Michael P
authors.initials: MP
authors.lastname: Murphy
authors.forename: Richard C
authors.initials: RC
authors.lastname: Hartley