title: The intact muscle lipid composition of bulls: an investigation by MALDI-TOF MS and 31P NMR.
abstract: The analysis of beef lipids is normally based on chromatographic techniques and/or gas chromatography in combination with mass spectrometry (GC/MS). Modern techniques of soft-ionization MS were so far scarcely used to investigate the intact lipids in muscle tissues of beef. The objective of the study was to investigate whether matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry and (31)P nuclear magnetic resonance (NMR) spectroscopy are useful tools to study the intact lipid composition of beef. For the MALDI-TOF MS and (31)P NMR investigations muscle samples were selected from a feeding experiment with German Simmental bulls fed different diets. Beside the triacylglycerols (TAGs), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatidylinositol (PI) species the MALDI-TOF mass spectra of total muscle lipids gave also intense signals of cardiolipin (CL) species. The application of different matrix compounds, 2,5-dihydroxybenzoic acid (DHB) and 9-aminoacridine (9-AA), leads to completely different mass spectra: 9-AA is particularly useful for the detection of (polar) phospholipids, whereas apolar lipids, such as cholesterol and triacylglycerols, are exclusively detected if DHB is used. Finally, the quality of the negative ion mass spectra is much higher if 9-AA is used.
authors.affiliation: Research Institute for Biology of Farm Animals, Research Unit of Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Dannenberger
authors.forename: Rosmarie
authors.initials: R
authors.lastname: Süss
authors.forename: Kristin
authors.initials: K
authors.lastname: Teuber
authors.forename: Beate
authors.initials: B
authors.lastname: Fuchs
authors.forename: Karin
authors.initials: K
authors.lastname: Nuernberg
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schiller
mesh_terms: D000585:Aminacrine / Q000032:analysis / Q000737:chemistry; D000818:Animals; D002417:Cattle; D005841:Gentisates / Q000032:analysis / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D018129:Phosphatidylinositol Phosphates / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis
chemical_list: D005841:Gentisates; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D018129:Phosphatidylinositol Phosphates; D014280:Triglycerides; C483858:phosphatidylethanolamine; D000585:Aminacrine; C010925:2,5-dihydroxybenzoic acid