title: In vitro reconstitution of Escherichia coli O86 O antigen repeating unit.
abstract: Polysaccharides constitute a major component of the bacterial cell surface. They play critical roles in the interactions between bacteria and the host environments, and consequently contribute to the virulence of pathogens. The lipopolysaccharide (LPS) found on the surface of gram-negative bacteria consists of three parts: lipid A, a core oligosaccharide, and the O antigen. The O antigen is the outermost part of LPS and contains multiple oligosaccharide repeating units. Biosynthesis of the O-repeating unit is the first committed step in LPS biosynthesis. We sequenced and characterized the O-antigen biosynthetic gene cluster of Escherichia coli serotype O86. Four glycosyltransferases encoded by the genes within the cluster were cloned and overexpressed. In vitro reconstitution of the O-repeating unit of E. coli 086 was achieved via using these enzymes.
authors.affiliation: Departments of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Nicholas
authors.initials: N
authors.lastname: Pettit
authors.forename: Wen
authors.initials: W
authors.lastname: Yi
authors.forename: Robert
authors.initials: R
authors.lastname: Woodward
authors.forename: Xianwei
authors.initials: X
authors.lastname: Liu
authors.forename: Wanyi
authors.initials: W
authors.lastname: Guan
authors.forename: Veer
authors.initials: V
authors.lastname: Bhatt
authors.forename: Jing Katherine
authors.initials: JK
authors.lastname: Song
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000737:chemistry; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D008055:Lipids; D008070:Lipopolysaccharides; D019081:O Antigens; D016695:Glycosyltransferases