[Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides].
OBJECTIVE
To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.


METHODS
Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.


RESULTS
The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.


CONCLUSIONS
Hairy root of E. ulmoides will be useful for the production of active components in aucubin.
Yang
Rui
R
School of Life Science, Southwest University, Chongqing 400715, China.
Zhang
Jiahui
J
Sun
Xuemei
X
Sun
Min
M
D003470:Culture Media / Q000378:metabolism; D031283:Eucommiaceae / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D005960:Glucosides / Q000032:analysis / Q000378:metabolism*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques
D003470:Culture Media; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; C006650:aucubin