title: Existence of NO-triggered vagal afferent activation in the rat gastric mucosa.
abstract: OBJECTIVE
  We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by the gastric vagus nerve, and we proposed that the stomach, like the duodenum, could "taste" luminal nutrients. Nitric oxide synthase (NOS) is widely distributed in the gastric mucosa, but the physiological role of mucosal NO is not well understood. In this study, we investigated the functional coupling of NO and vagal nerve endings in the gastric mucosa.
  
  
  METHODS
  For electrophysiological recordings, male Sprague-Dawley rats were anesthetized with urethane, and afferent nerve responses of rat vagal gastric branches to a NO donor were monitored.
  
  
  RESULTS
  Intravenous application of 100microg/kg sodium nitroprusside (SNP) transiently increased afferent nerve discharges of the rat ventral gastric vagus, which was followed by rapid changes in blood pressure. High doses of SNP (>300microg/kg, i.v.) showed a biphasic increase in afferent discharges. Secondary activation of the vagal afferent continued even after blood pressure returned to basal levels. SNP-evoked afferent responses were abolished by mucosal 5-HT depletion using p-cholorophenylalanine and were inhibited by pre- and post-treatment with the 5-HT(3) antagonist granisetron.
  
  
  CONCLUSIONS
  These pharmacological results strongly indicate that NO-triggered 5-HT release is coupled to vagal afferent activation in the rat gastric mucosa.
authors.affiliation: Physiology & Nutrition Group, Institute of Life Sciences, Ajinomoto Co., Inc. Kawasaki 210-8681, Japan. hisayuki_uneyama@ajinomoto.com
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Uneyama
authors.forename: Akira
authors.initials: A
authors.lastname: Niijima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kitamura
authors.forename: Kunio
authors.initials: K
authors.lastname: Torii
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014630:Vagus Nerve / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide