Different bacteria species lipopolysaccharide co-exposure with Pseudomonas exotoxin A on multiple organ injury induction.
The present study investigated the effect of different bacterial species lipopolysaccharide plus Pseudomonas exotoxin A (LPS/PEA) on the induction of multiple organ injury (MOI). Rats were injected with various LPS from Salmonella (SAE, SAT), E. coli (EB4, EB5), or P. aeruginosa (PAL) and PEA showed a greater mortality in the SAE/PEA and SAT/PEA groups. Histological alterations, serum enzymes, and cytokines changes were severer in the SAE/PEA group than the EB4/PEA or PAL/PEA group. EB4/PEA and PAL/PEA failed to induce MOI, even at the LPS doses increased up to 2-4- and 4-8-fold, respectively. Rats co-treated with Salmonella lipid A/PEA developed severer MOI than the E. coli lipid A/PEA. The results indicated the critical roles of MOI induction, which were related to LPS derived from appropriate bacterial species.
Chuang
Hsiao-Li
HL
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
Chiu
Chien-Chao
CC
Chen
Ter-Hsin
TH
Chen
Hans Hsien-Chuan
HH
Chu
Ying-Yueh
YY
Huang
Yen-Te
YT
D036002:ADP Ribose Transferases / Q000633:toxicity*; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D004306:Dose-Response Relationship, Immunologic; D005098:Exotoxins / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009102:Multiple Organ Failure / Q000276:immunology* / Q000382:microbiology* / Q000401:mortality; D009104:Multiple Trauma / Q000276:immunology* / Q000382:microbiology* / Q000401:mortality; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D015996:Survival Rate; D037521:Virulence Factors / Q000633:toxicity*; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D008070:Lipopolysaccharides; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A