title: First isolation of an H1N1 avian influenza virus from wild terrestrial non-migratory birds in Argentina.
abstract: A type A avian influenza (AI) virus was isolated from dead or severely ill red-winged tinamous (Rhynchotus rufescens) found in a hunting ground in April 2008 in Argentina. The subtype of A/red-winged tinamou/Argentina/MP1/2008 was determined as H1N1 by sequence analysis. The cleavage site of the viral hemagglutinin corresponded to a low pathogenic influenza virus, although the clinical presentation and pathological studies suggest that the virus was pathogenic for red-winged tinamous. Phylogenetic analysis of the viral genome suggested that while the hemagglutinin and neuraminidase genes were related to AIV from North America, the internal genes were most closely related to other South American isolates. These findings support the postulated South American phylogenetic lineage for AIV PB2, PB1, PA, M and NS genes, and suggest that the evolutionary pathways of HA and NA genes involve exchanges between the Northern and Southern hemispheres.
authors.affiliation: Centro de Virología Animal, Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, (1440) Buenos Aires, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Alvarez
authors.forename: Rosana
authors.initials: R
authors.lastname: Mattiello
authors.forename: Pierre
authors.initials: P
authors.lastname: Rivailler
authors.forename: Ariel
authors.initials: A
authors.lastname: Pereda
authors.forename: Charles T
authors.initials: CT
authors.lastname: Davis
authors.forename: Lorena
authors.initials: L
authors.lastname: Boado
authors.forename: Elisa
authors.initials: E
authors.lastname: D'Ambrosio
authors.forename: Sebastian
authors.initials: S
authors.lastname: Aguirre
authors.forename: Cora
authors.initials: C
authors.lastname: Espinosa
authors.forename: José
authors.initials: J
authors.lastname: La Torre
authors.forename: Ruben
authors.initials: R
authors.lastname: Donis
authors.forename: Nora
authors.initials: N
authors.lastname: Mattion
mesh_terms: D000818:Animals; D001118:Argentina; D001483:Base Sequence; D001717:Birds / Q000821:virology*; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis / Q000473:pathology; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase