Protective effects of dieckol isolated from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells.
The effect of dieckol, one of phlorotannin polyphenol compound purified from Ecklonia cava (E. cava) against high glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30mM) treatment induced HUVECs cell death, but dieckol, at concentration 10 or 50microg/ml, significantly inhibited the high glucose-induced cytotoxicity. Furthermore, treatment with dieckol dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF-kB) proteins in HUVECs, but dieckol treatment reduced the overexpressions of these proteins. These findings indicate that dieckol is a potential therapeutic agent that will reduce the damage caused by hyperglycemia-induced oxidative stress associated with diabetes.
Lee
Seung-Hong
SH
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Han
Ji-Sook
JS
Heo
Soo-Jin
SJ
Hwang
Ji-Young
JY
Jeon
You-Jin
YJ
D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology*; D005947:Glucose / Q000008:administration & dosage / Q000633:toxicity*; D006801:Humans; D015227:Lipid Peroxidation; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology
D001572:Benzofurans; D016328:NF-kappa B; C503840:dieckol; D005947:Glucose