The clonogenic potential of hematopoietic stem cells and mesenchymal stromal cells in various hematologic diseases: a pilot study.
OBJECTIVE
Mesenchymal stromal cells (MSC) are the most popular cells used in regenerative medicine and biotechnology. The clonogenic potential of these cells is defined by colony-forming unit-fibroblasts (CFU-F). It is well known that there is an interaction between hematopoietic cells and stromal cells in disease formation pathogenesis. Therefore we hypothesized that there should be a quantitative and qualitative relationship between MSC colonies (CFU-F) and hematopoietic stem cell colonies (colony-forming unit-granulocyte-macrophages; CFU-GM) among patients with and without hematologic diseases.


METHODS
Forty-two patients were included in this study. Patients were divided into three groups: group A, patients with hematologic malignancies (n =20); group B, patients with bone marrow (BM) failure (n =11); group C, patients without hematologic diseases (n =11). BM aspirates were plated in different densities for CFU-F culture. The plating density was the same for CFU-GM culture.


RESULTS
CFU-GM colonies grew in 90% of group A cells and all of group B and C cells (P= 0.0001). CFU-F colonies became visible on the ninth day of plating in group A and on the eight day in groups B and C. There was no statistically significant difference between the groups for the duration of CFU-F colony formation (P= 0.12). There were differences in the morphology of the colonies among the groups.


CONCLUSIONS
This is the first study that has compared the clonogenic potential of stromal cells and hematopoietic stem cells in the same subjects with and without hematologic diseases. No correlation was shown between the clonogenic potential of stromal cells and hematopoietic cells.
Kurt Yüksel
Meltem
M
Department of Hematology, Ankara Oncology Research and Education Hospital, Ankara University School of Medicine, Ankara, Turkey. meltemkurt@hotmail.com
Topçuoğlu
Pervin
P
Kurdal
Mevlude
M
Ilhan
Osman
O
D000328:Adult; D000368:Aged; D001855:Bone Marrow Diseases / Q000503:physiopathology / Q000628:therapy; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods; D005260:Female; D055014:Granulocyte-Macrophage Progenitor Cells / Q000502:physiology; D006402:Hematologic Diseases / Q000628:therapy; D019337:Hematologic Neoplasms / Q000503:physiopathology / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D010865:Pilot Projects; D017154:Stromal Cells / Q000502:physiology* / Q000637:transplantation*; D055815:Young Adult