A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies.
A new methodology has been developed to produce sections as thin as 5 microm of stink bug (Heteroptera: Pentatomidae) mouthparts for morphological studies. Heads of the southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) were fixed on Bouin solution, and treated with EDTA (ethylenediamine tetraacetic acid 7%) in an ice bath within a microwave oven for 0.5, 1, 2, 3, 4, and 5 h. The chitin structures and labium soft parts were well preserved after 0.5h EDTA treatment. Longer times of exposure to EDTA and microwave irradiation caused damage to mouthpart structures.
Depieri
Rogério A
RA
Department of Zoology, Federal University of Paraná, P.O. Box 19020, Curitiba, PR, 81531-980, Brazil.
Andrade
Célia G T J
CG
Panizzi
Antônio R
AR
D000818:Animals; D004492:Edetic Acid; D006430:Hemiptera / Q000033:anatomy & histology*; D008872:Microwaves; D009055:Mouth / Q000033:anatomy & histology*; D014021:Tissue Preservation / Q000379:methods*
D004492:Edetic Acid