Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs).
OBJECTIVE
We speculated that distinct angiogenic profiles are involved in idiopathic interstitial pneumonias (IIPs) in comparison with interstitial pneumonias associated with collagen vascular disease (CVD-IPs). This hypothesis was investigated by measuring the expression of a cardinal biologic axis, the vascular endothelial growth factor (VEGF)-stromal derived growth factor [SDF-1alpha, transcripts 1 and 2 (TR1 and TR2)] and receptor, CXCR4 and the angiogenetic receptors CXCR2 and CXCR3 in bronchoalveolar lavage fluid (BALF) in both conditions.


METHODS
We studied prospectively 25 patients with fibrotic IIPs (f-IIPs) [20 with idiopathic pulmonary fibrosis (IPF) and 5 with idiopathic non-specific interstitial pneumonia (NSIP)] and 16 patients with CVD-IPs. mRNA expression was measured by Real-Time RT-PCR and protein was evaluated by Western Blotting.


RESULTS
A significantly greater value has been detected in SDF-1alpha-TR1 mRNA expression levels of CVD-IPs (p=0.05) in comparison with IPF group. A similar trend has been also detected in protein expression in favor of CVD-IP group. In addition, VEGF mRNA levels have been found significantly increased in CVD-IPs in comparison with the NSIP group (p=0.05). No significant difference has been found in SDF-1alpha-TR2-CXCR4 mRNA and CXCR2-CXCR3 between the two groups.


CONCLUSIONS
These results showed increased expression of SDF-1alpha in CVD-IPs, suggesting different angiogenic procedures. Further studies are needed in order to better explore the angiogenetic pathway in these disorders.
Margaritopoulos
Giorgos A
GA
Department of Thoracic Medicine, Interstitial Lung Disease Unit, University Hospital, Medical School, University of Crete, Heraklion 71110, Crete, Greece.
Antoniou
Katerina M
KM
Soufla
Giannoula
G
Karagiannis
Konstantinos
K
Proklou
Athanasia
A
Lasithiotaki
Ismini
I
Tzanakis
Nikolaos
N
Spandidos
Demetrios A
DA
Siafakas
Nikolaos M
NM
D000368:Aged; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D054377:Chemokine CXCL12 / Q000235:genetics*; D003095:Collagen Diseases / Q000235:genetics / Q000503:physiopathology; D005260:Female; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000235:genetics* / Q000503:physiopathology; D017563:Lung Diseases, Interstitial / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics; D023063:Receptors, Interleukin-8B / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014652:Vascular Diseases / Q000235:genetics / Q000503:physiopathology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D054377:Chemokine CXCL12; D012333:RNA, Messenger; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4; D023063:Receptors, Interleukin-8B; D042461:Vascular Endothelial Growth Factor A