title: Low-high season variation in Plasmodium falciparum erythrocyte binding antigen 175 (eba-175) allelic forms in malaria endemic area of Burkina Faso.
abstract: OBJECTIVE
  To assess the impact of seasonal variation on the distribution of the eba-175 allelic forms in the area where malaria transmission is markedly seasonal.
  
  
  METHODS
  Blood samples were collected from 291 and 239 children under 5 years of age during the low and the high malaria transmission season, respectively, in four villages named Dawelgué, Kounda, Tanghin and Watenga of Saponé Health District, then screened for eba 175 F- and C- alleles by nested PCR analysis.
  
  
  RESULTS
  F- alleles were more prevalent than C-alleles in the low [0.66 vs. 0.34 (P < 0.0001)] and high transmission season [0.67 vs. 0.33 (P < 0.0001)]. No significant seasonal variation was observed in the distribution of the two alleles. However, according to Sewall Wright rules, the population pairwise F(ST) values, between Dawelgué and Tanghin during the low transmission season (F(ST_) value = 0.10415, P-value = 0.0090 and during the high season (F(ST_)value = 0.08244, P-value < 0.00001), between Tanghin and Watenga during the low season (F(ST) value = 0.07414, P-value = 0.009) indicated a moderate but statistically significant genetic differentiation.
  
  
  CONCLUSIONS
  Although there was a moderate but significant genetic differentiation between some study villages at different times of the year, this study result in the seasonal stability of eba-175 allele's distribution in the study area.
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
authors.forename: Issiaka
authors.initials: I
authors.lastname: Soulama
authors.forename: Edith C
authors.initials: EC
authors.lastname: Bougouma
authors.forename: Amidou
authors.initials: A
authors.lastname: Diarra
authors.forename: Issa
authors.initials: I
authors.lastname: Nebie
authors.forename: Sodiomon B
authors.initials: SB
authors.lastname: Sirima
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002050:Burkina Faso / Q000453:epidemiology; D002675:Child, Preschool; D019353:Endemic Diseases; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D008297:Male; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics*; D012423:Rural Health / Q000706:statistics & numerical data; D012621:Seasons
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium