Bile acid transport correlative protein mRNA expression profile in human placenta with intrahepatic cholestasis of pregnancy.
OBJECTIVE
To analyze the expression of bile acid transport correlative proteins in the placenta of patients with intrahepatic cholestasis of pregnancy (ICP).


METHODS
This case-control study was performed in the Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China from March 2007 to October 2008. Real time reverse transcriptase polymerase chain reaction was applied for the mRNA expression measurement of 8 bile acid transport correlative proteins, organic anion transporting polypeptide (OATP)1A2, OATP1B1, multidrug resistance protein (MRP)1, MRP2, anion exchanger (AE)2, bile salt export pump (BSEP), multidrug resistance 3, and familial intrahepatic cholestasis (FIC)1, in normal human placentas (n=20) and those with ICP (n=20).


RESULTS
All the transcripts except OATP1B1 and BSEP were detected. Both OATP1A2 and AE2 mRNA were higher while FIC1 was lower in ICP patients.


CONCLUSIONS
The alteration of bile acid transport correlative proteins OATP1A2, AE2, and FIC1 may be involved in the fetal cholestasis of ICP.
Cui
Tao
T
Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Sichuan, China.
Liu
Yufang
Y
Men
Xiaoliang
X
Xu
Zhou
Z
Wu
Lin
L
Liu
Shuyun
S
Xing
Aiyun
A
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000328:Adult; D001647:Bile Acids and Salts / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D002585:Cesarean Section; D002681:China; D002780:Cholestasis, Intrahepatic / Q000175:diagnosis / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D027361:Organic Anion Transporters / Q000235:genetics; D010920:Placenta / Q000737:chemistry / Q000378:metabolism; D021041:Placental Circulation / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis / Q000235:genetics*; D011256:Pregnancy Outcome; D011263:Pregnancy Trimester, Third; D011336:Probability; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D018528:ATP-Binding Cassette Transporters; D001647:Bile Acids and Salts; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger