Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma.
We compared microRNA profiles between choriocarcinoma and non-cancerous trophoblasts, and revealed that miR-199b was underexpressed in choriocarcinoma. By computational prediction and microarray studies, SET (protein phosphatase 2A inhibitor) was shown to be one of the target genes regulated by miR-199b. Ectopic expression of miR-199b inhibited endogenous SET protein levels and the activity of the luciferase reporter containing the 3'-UTR of SET. Further comparisons of formalin-fixed paraffin-embedded human choriocarcinoma, mole, and non-cancer trophoblast tissues confirmed the initial findings of low miR-199b expression and SET upregulation in choriocarcinomas, suggesting that microRNA-dysregulated SET protein may account for the rapid growth seen with choriocarcinomas.
Chao
Angel
A
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taiwan.
Tsai
Chia-Lung
CL
Wei
Pei-Chi
PC
Hsueh
Swei
S
Chao
An-Shine
AS
Wang
Chin-Jung
CJ
Tsai
Chi-Neu
CN
Lee
Yun-Shien
YS
Wang
Tzu-Hao
TH
Lai
Chyong-Huey
CH
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins; D005260:Female; D056488:Histone Chaperones / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D011247:Pregnancy; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D014327:Trophoblasts / Q000737:chemistry; D014594:Uterine Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology
D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D035683:MicroRNAs; C513473:SET protein, human; D014157:Transcription Factors; C531408:mirn199 microRNA, human