A method for rapid generation of transgenic animals to evaluate testis genes during sexual maturation.
In certain forms of idiopathic infertility, there is failure of follicle stimulating hormone (FSH) and testosterone (T) to initiate spermatogenesis despite the presence of Sertoli cells and germ cells in the testis. In postnatal rats (up to 11 days of age) and infant monkeys (3-4 months old), robust division and differentiation of spermatogonial stem cells is not discerned, even though serum levels of FSH and T are similar to those found during adulthood. Lack of spermatogenesis together with normal hormone levels is a situation similar to that found in certain categories of male infertility. To investigate this intriguing situation, Sertoli cells were cultured from infant and pubertal rats and monkeys and differential gene expression by testicular Sertoli cells was evaluated by DNA microarray using the Agilent microarray system. To determine the role of candidate genes in regulation of spermatogenesis, transgenic animals over-expressing these genes must be generated. However, present techniques for generation of transgenic animals have limited utility for production of several transgenic animals within a short period of time. Therefore, we have developed a technique for making transgenic animals by the testicular route which is less labor intensive and less time consuming. This technique is also ethically superior since fewer mice are required than in existing alternative methods of transgenesis.
Majumdar
Subeer S
SS
Division of Cellular Endocrinology, National Institute of Immunology, New Delhi, India. subeer@nii.res.in
Usmani
Abul
A
Bhattacharya
Indrashis
I
Sarda
Kanchan
K
Gautam
Mukkesh
M
Sharma
Deepika
D
Basu
Sayon
S
Dhup
Suveera
S
D000818:Animals; D030801:Animals, Genetically Modified; D000831:Animals, Newborn; D018274:Electroporation; D020869:Gene Expression Profiling; D000882:Haplorhini; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D012741:Sexual Maturation / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism*