title: The immunohistochemical characterization of human fetal olfactory bulb and olfactory ensheathing cells in culture as a source for clinical CNS restoration.
abstract: Clinical studies have expanded the therapeutic olfactory ensheathing cells (OECs) transplantation to different human Central Nervous System (CNS) diseases. In fact, the OEC transplantation in clinic is a mixture of olfactory bulb cells; they even have not demonstrated that they have such a subpopulation yet. However, as a source of OECs transplantation, the development and identification of human fetal OECs are still need more understanding, because some surgery try to restoration CNS injury with a more purity of OEC cultures generated by a number of different procedures. In this article, twelve human fetal olfactory bulb (OB) samples were obtained from six fetuses in 20 weeks of gestation, it was studied by immunofluorescence on histological sections and cultured cells with multiple antibodies under confocal microscopy. The P75NTR positive OB-OECs (olfactory ensheathing cell from the olfactory bulb) were present in both outer olfactory nerve layers and glomerular layer. The percentage of OB cells in culture, about 22.31 was P75NTR positive, 45.77 was S100beta, and 31.92 was GFAP. P75NTR and GFAP were coexpressed with S100beta, respectively; however, P75NTR was not coexpressed with GFAP in human fetal OECs. It is suggested that the localization and development of human OECs in OB are different to those in rodent, and the P75NTR immunohistological staining is still necessary to identify and characterize human fetal OECs in culture before transplantation.
authors.affiliation: Beijing Hongtianji Neuroscience Academy, Xi-xia-zhuang, Ba-da-chu, Shi-jing-shan District, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Jiang
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dongming
authors.initials: D
authors.lastname: Sun
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Wise
authors.initials: W
authors.lastname: Young
authors.forename: Hongyun
authors.initials: H
authors.lastname: Huang
authors.forename: Changman
authors.initials: C
authors.lastname: Zhou
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002490:Central Nervous System; D019084:Fluorescent Antibody Technique, Indirect; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009830:Olfactory Bulb / Q000196:embryology* / Q000378:metabolism; D009832:Olfactory Nerve / Q000196:embryology* / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D009418:S100 Proteins