title: [Identification of differentially expressed genes in different anomalies of Glycyrrhiza uralensis by cDNA-AFLP method].
abstract: OBJECTIVE
  To understand the molecular mechanism in genetic variation within species, and identify the differentially expressed genes among 8 anomalies of Glycyrrhiza uralensis under the same genetic background and cultivated environment.
  
  
  METHODS
  The differentially expressed genes in roots of different anomalies of G. uralensis in vigorous growing stage were identified by cDNA-AFLP and confirmed by reverse-Northern hybridization. Their functions were inferred through bioinformatics method.
  
  
  RESULTS
  Fourteen differentially expressed genes were identified, among which, the function of 6 were unknown, and the rest were involved in antibacterium, regulating metabolism, enhancing the heat resistance ability, increasing the nitrogen fixation ability, and other bioprocesses.
  
  
  CONCLUSIONS
  For the first time, the differentially expressed genes were cloned from the different anomalies of G. uralensis. This provided the basis for the screening of fine anomalies and varieties, and the research of functional genomics.
authors.affiliation: School of Traditional Chinese Medicines, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yang
authors.forename: Shengli
authors.initials: S
authors.lastname: Wei
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Chunrong
authors.initials: C
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D029882:Glycyrrhiza uralensis / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins