Insulin resistance in insulin-resistant and diabetic hamsters (Mesocricetus auratus) is associated with abnormal hepatic expression of genes involved in lipid and glucose metabolism.
Fat-induced hepatic insulin resistance (FIHIR) in obesity induced by high-fat diet leads to ectopic lipid accumulation and may contribute to the pathogenesis of type 2 diabetes. We examined the alterations in hepatic gene expression involved in FIHIR by using obese insulin-resistant and diabetic hamsters that received high-fat diet with or without low-dose streptozotocin. Microarray analysis and confirmatory real-time RT-PCR indicated that increased mRNA levels of sterol regulatory element-binding proteins (SREBPs) and decreased mRNA levels of liver X receptor (LXRalpha) and peroxisome-proliferator-activated receptor (PPARalpha) occurred in FIHIR in insulin-resistant and diabetic hamsters. Expression levels of hepatic LXRalpha, SREBPs, and PPARalpha differed significantly between insulin-resistant and diabetic hamsters. Expression of LXRalpha, SREBPs, and PPARalpha all change in FIHIR associated with hepatic lipid accumulation in insulin-resistant and diabetic hamsters in which disease is induced by high-fat diet and streptozotocin injection.
Li
Guosheng
G
Department of Pathology, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.
Liu
Xuhan
X
Zhu
Hua
H
Huang
Lan
L
Liu
Yali
Y
Ma
Chunmei
C
Qin
Chuan
C
D000818:Animals; D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000032:analysis; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage; D050171:Dyslipidemias / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018149:Glucose Intolerance / Q000097:blood / Q000139:chemically induced / Q000235:genetics*; D007333:Insulin Resistance / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D008647:Mesocricetus; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051779:Sterol Regulatory Element Binding Proteins / Q000235:genetics / Q000378:metabolism
D001786:Blood Glucose; D004041:Dietary Fats; D008055:Lipids; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D047493:PPAR alpha; D012333:RNA, Messenger; D051779:Sterol Regulatory Element Binding Proteins