In vitro cytotoxicity and drug release properties of pH- and temperature-sensitive core-shell hydrogel microspheres.
A simple method has been developed to prepare smart P(N,N-diethylacrylamide-co-methacrylic acid) (P(DEA-co-MAA)) microspheres that consist of well-defined temperature-sensitive cores and pH sensitive shells. The microgels have been prepared by surfactant-free emulsion polymerization using water as the solvent. The core-shell hydrogel microspheres have been characterized by Fourier transform infrared (FTIR) spectroscopy, UV spectrometry, dynamic light scattering (DLS) and transmission electron micrograph (TEM). Preliminary characterization of the biocompatibility of hydrogel microspheres has been done by the cytotoxicity assays using the HeLa human breast cancer cell line as probes. The in vitro drug release indicates that drug release rate, encapsulation efficiency (EE) and release kinetics depend upon the pH value and copolymer composition. According to this study, the hydrogel microspheres based on P(DEA-co-MAA) could serve as suitable candidate for drug site-specific carrier in intestine.
Ma
Liwei
L
State Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Liu
Mingzhu
M
Liu
Hongliang
H
Chen
Jun
J
Cui
Dapeng
D
D000178:Acrylamides / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008027:Light; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000633:toxicity; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D014867:Water / Q000737:chemistry
D000178:Acrylamides; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D011108:Polymers; D011109:Polymethacrylic Acids; D012997:Solvents; C428028:poly(N,N-diethylacrylamide); D014867:Water; C030613:polymethacrylic acid; D012710:Serum Albumin, Bovine