title: Determination of the 5'-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells.
abstract: Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5'-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the genotype 3 variants, it was found that the subgenomic RNA of genotype 3 and 4 HEVs initiates exclusively at nucleotide 5122 with the common sequence of 5'-GC, which is identical to that of the prototype genotype 1 HEV.
authors.affiliation: Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ichiyama
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.forename: Toshinori
authors.initials: T
authors.lastname: Tanaka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nagashima
authors.lastname: Jirintai
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamoto
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003470:Culture Media; D017077:Culture Media, Conditioned; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D016751:Hepatitis E / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012315:RNA Caps / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D003470:Culture Media; D017077:Culture Media, Conditioned; D012315:RNA Caps; D012367:RNA, Viral; D014764:Viral Proteins