Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India.
Classical swine fever (CSF), a highly contagious viral disease of pigs, is endemic in India. As there is no information concerning the accurate genetic typing of classical swine fever virus (CSFV) isolates in India, 16 CSF viruses isolated during 2005-2007 from domestic pigs in different districts of Assam were typed in 5' UTR (150 nucleotides). To confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in E2 (190 nt) and NS5B gene (409 nt) regions. Phylogenetic analysis revealed that all the 16 CSFV isolates analyzed belonged to group 1 and subgroup 1.1 in contrast to the situation in other Asian countries. Additionally, analysis in E2 and NS5B region placed the Indian isolates in a clearly separated clade within subgroup 1.1. The results suggest that subgroup 1.1 CSF viruses are currently circulating in India, which is important for epidemiology and control of CSF.
Sarma
Dilip Kumar
DK
Assam Agricultural University, Khanapara, Guwahati, Assam, India.
Mishra
Niranjan
N
Vilcek
Stefan
S
Rajukumar
Katherukamem
K
Behera
Sthita Pragnya
SP
Nema
Ram Kumar
RK
Dubey
Pooja
P
Dubey
Shiv Chandra
SC
D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D007194:India; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins