Anti-obesity effect of Blumea balsamifera extract in 3T3-L1 preadipocytes and adipocytes.
Obesity, the leading metabolic disease in the world, is a serious health problem in industrialized countries. We investigated the anti-obesity effect of Blumea balsamifera extract on adipocyte differentiation of 3T3-L1 preadipocytes and anti-obesity effect of 3T3-L1 adipocytes. We found that treatment with an extract of Blumea balsamifera suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity without affecting cell viability in 3T3-L1 preadipocytes and adipocytes. Furthermore, Blumea balsamifera extract brought significant attenuation of expressions of key adipogenic transcription factors, including peroxisome proliferator-activated receptor (PPAR)gamma, CCAAT element binding protein (C/EBPs) and leptin, however, induced up-regulation of adiponectin at the protein level in 3T3-L1 preadipocytes and adipocytes. These results suggest that Blumea balsamifera extract may block adipogenesis, at least in part, by decreasing key adipogenic transcription factors in 3T3-L1 preadipocytes and may have antiatherogenic, anti-inflammatory, and antidiabetic effects through up-regulation of adiponectin in 3T3-L1 adipocytes.
Kubota
Hiroaki
H
Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.
Kojima-Yuasa
Akiko
A
Morii
Risako
R
Huang
Xuedan
X
Norikura
Toshio
T
Rho
Sook-Nyung
SN
Matsui-Yuasa
Isao
I
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001391:Azo Compounds / Q000737:chemistry; D015153:Blotting, Western; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D051379:Mice; D009765:Obesity / Q000517:prevention & control; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D052242:Adiponectin; D001391:Azo Compounds; D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D020738:Leptin; D008055:Lipids; D047495:PPAR gamma; D010936:Plant Extracts; D005993:Glycerolphosphate Dehydrogenase; C011049:oil red O