Protein stabilization and the Hofmeister effect: the role of hydrophobic solvation.
Using the IGg binding domain of protein L from Streptoccocal magnus (ProtL) as a case study, we investigated how the anions of the Hofmeister series affect protein stability. To that end, a suite of lysine-to-glutamine modifications were obtained and structurally and thermodynamically characterized. The changes in stability introduced with the mutation are related to the solvent-accessible area of the side chain, specifically to the solvation of the nonpolar moiety of the residue. The thermostability for the set of ProtL mutants was determined in the presence of varying concentrations (0-1 M) of six sodium salts from the Hofmeister series: sulfate, phosphate, fluoride, nitrate, perchlorate, and thiocyanate. For kosmotropic anions (sulfate, phosphate, and fluoride), the stability changes induced by the cosolute (encoded in m(3)=deltaDeltaG(0)/deltaC(3)) are proportional to the surface changes introduced with the mutation. In contrast, the m(3) values measured for chaotropic anions are much more independent of such surface modifications. Our results are consistent with a model in which the increase in the solution surface tension induced by the anion stabilizes the folded conformation of the protein. This contribution complements the nonspecific and weak interactions between the ions and the protein backbone that shift the equilibrium toward the unfolded state.
Tadeo
Xavier
X
Structural Biology Unit, Centro de Investigación Cooperativa bioGUNE, Derio, Spain.
López-Méndez
Blanca
B
Castaño
David
D
Trigueros
Tamara
T
Millet
Oscar
O
D000838:Anions; D001703:Biophysics / Q000379:methods*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008239:Lysine / Q000737:chemistry; D009154:Mutation; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013291:Streptococcus / Q000378:metabolism; D013816:Thermodynamics
D000838:Anions; D011506:Proteins; D012997:Solvents; D005973:Glutamine; D008239:Lysine