title: The lipid A of Burkholderia multivorans C1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model.
abstract: Cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. Burkholderia multivorans and Burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. Lipopolysaccharide endotoxins (LPSs) are among the foremost factors of pathogenesis of Gram-negative infection and, in particular, lipid A is the endotoxic portion of LPS responsible for eliciting host innate immune response. In this work, the complete primary structure of the lipid A from B. multivorans C1576 has been defined and, further, its pro-inflammatory activity in a cystic fibrosis airways model is shown. The structure of B. multivorans lipid A was attained by chemical, mass spectrometry and nuclear magnetic resonance analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the airway epithelial IB3-1 cells, carrying the ΔF508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico, Napoli, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ieranò
authors.forename: Paola
authors.initials: P
authors.lastname: Cescutti
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Leone
authors.forename: Alessandro
authors.initials: A
authors.lastname: Luciani
authors.forename: Roberto
authors.initials: R
authors.lastname: Rizzo
authors.forename: Valeria
authors.initials: V
authors.lastname: Raia
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Luigi
authors.initials: L
authors.lastname: Maiuri
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019117:Burkholderia / Q000737:chemistry*; D018938:Caco-2 Cells; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D054884:Host-Pathogen Interactions; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008050:Lipid A / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D009298:Nasal Polyps / Q000188:drug therapy; D021241:Spectrometry, Mass, Electrospray Ionization; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D008050:Lipid A; D014409:Tumor Necrosis Factor-alpha