[Structure characteristics of the chloroplast rpS16 intron in Allium sativum and related Allium species].
For the first time the chloroplast rpS16 intron sequences in A. sativum accessions with different ecologo-geographical origins and related Allium species have been characterized. The main stem-loop consensus sequences and boundaries ofsix domains have been identified and the most probable secondary structure model of the intron pre-RNA has been predicted. Allium rpS16 introns have been characterized by mutation rate heterogeneity between structure regions of all six domains. Domains II and IV of the intron are shown to be more variable with transition predominance in I, III, V and VI domain sequences. In addition to structure elements typical for group IIB introns the Allium specific micro- and macrostructural alterations have been revealed. The 290 nucleotide deletion of domains III-IV sequences and of the part of the domain V has been revealed in A. altaicum, A. fistulosum, A. schoenoprasum rpS16 intron sequences. Several nucleotide substitutions and extensive length mutations result in secondary structure deviation from the consensus model of group II introns.
Ryzhova
N N
NN
Kholda
O A
OA
Kochieva
E Z
EZ
D005737:Garlic / Q000235:genetics* / Q000378:metabolism; D054628:Genome, Chloroplast / Q000502:physiology*; D007438:Introns / Q000502:physiology*; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D013329:Structure-Activity Relationship
D010940:Plant Proteins; D012322:RNA Precursors; D018749:RNA, Plant; D012269:Ribosomal Proteins