title: Antimicrobial activity of aminoreductone against Helicobacter pylori.
abstract: Anti-Helicobacter pylori (H. pylori) effects of aminoreductone (AR), a Maillard reaction product, were evaluated in this study. AR effectively inhibited the growth of all 24 strains (19 clinical isolates and 5 isogenic mutants) irrespective of susceptibility to antibiotics and clinical manifestation. The minimum inhibitory concentration (MIC) of AR ranged from 0.5 to 5 mM. A killing assay with multiples of MIC was performed, demonstrating that the killing activity of AR was significantly higher than that of its derived melanoidin, an inhibitor of H. pylori urease-gastric mucin adherence, formed in the final stage of the Maillard reaction. These significant effects of AR on H. pylori were observed even in acidic conditions (pH 3). At most, 25 mM AR effectively exhibited bactericidal activity in all strains. These results rise up the possibility that foods containing AR, such as milk and dairy products, are valuable sources for preventing colonization of H. pylori in the stomach and its associated tissue damages.
authors.affiliation: Faculty of Agriculture, Kochi University, Monobe B-200, Nankoku, Japan.
authors.forename: Vu Thu
authors.initials: VT
authors.lastname: Trang
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Hayato
authors.initials: H
authors.lastname: Kudo
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Aoki
authors.forename: Shinya
authors.initials: S
authors.lastname: Katsuno
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shimamura
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ukeda
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; C017945:scorbamic acid; D001205:Ascorbic Acid