Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit.
Loquat (Eriobotrya japonica Lindl.) is a subtropical fruit, with some cultivars such as 'Luoyangqing' (LYQ) susceptible to chilling injury (CI), while others such as 'Baisha' (BS) are resistant. Although loquats are non-climacteric, modulation of ethylene has an effect on ripening-related post-harvest CI. Therefore the role of ethylene signalling in the development of CI was investigated in fruit of both the LYQ and BS cultivars. Three ethylene receptor genes, one CTR1-like gene, and one EIN3-like gene were isolated and characterized in ripening fruit. All of these genes were expressed differentially within and between fruit of the two cultivars. Transcripts either declined over fruit development (EjERS1a in both cultivars and EjEIL1 in LYQ) or showed an increase in the middle stages of fruit development before declining (EjETR1, EjERS1b, and EjCTR1 in both cultivars and EjEIL1 in BS). The main cultivar differences were in levels rather than in patterns of expression during post-harvest storage. EjETR1, EjCTR1, and EjEIL1 genes showed increased expression in response to low temperature and this was particularly notable for EjETR1, and EjEIL1 during CI development in LYQ fruit. The genes were also differentially responsive to ethylene treatment, 1-methycyclopropene (1-MCP) and low temperature conditioning, confirming a role for ethylene in regulation of CI in loquat fruit.
Wang
Ping
P
Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China.
Zhang
Bo
B
Li
Xian
X
Xu
Changjie
C
Yin
Xueren
X
Shan
Lanlan
L
Ferguson
Ian
I
Chen
Kunsong
K
D003080:Cold Temperature; D003521:Cyclopropanes / Q000494:pharmacology; D031983:Eriobotrya / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000187:drug effects
D003521:Cyclopropanes; D005030:Ethylenes; D010940:Plant Proteins; D008031:Lignin; C036216:ethylene; C412563:1-methylcyclopropene