[Roles of OxyRxoo, a transcriptional regulator of Xanthomonas oryzae pv. oryzae in regulation of detoxification of hydrogen peroxide].
OBJECTIVE
OxyRxoo is a homologue of OxyR from Xanthomonas oryzae pv. oryzae (Xoo), the pathogen of bacterial blight of rice. To elucidate the role of OxyRxoo in detoxification of hydrogen peroxide.


METHODS
OxyRxoo was studied by gene cloning, sequencing, deletion,complement and phenotype analysis.


RESULTS
Compared to those of the wild-type strain PXO99(A), there was no difference in bacterial growth in vitro of delta oxyRxoo, but delta oxyRxoo is more sensitive to H2O2 with reduced catalase activity. In the presence of H2O2, the expression of catalase genes (ahpCxoo, catBxoo, katExoo and srpAxoo) was significantly down-regulated, while the expression of oxyRxoo was up-regulated in delta oxyRxoo.


CONCLUSIONS
OxyRxoo functions as a transcription regulator in mediating and controlling H2O2 detoxification in Xoo.
Zhang
Jiantang
J
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. zh_jiantang@126.com
Gao
Jie
J
Wu
Maosen
M
Chen
Huamin
H
He
Chenyang
C
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D012097:Repressor Proteins; D006861:Hydrogen Peroxide; D002374:Catalase