Characterization of two NADPH: cytochrome P450 reductases from cotton (Gossypium hirsutum).
Cytochrome P450 monooxygenases (P450s) are commonly involved in biosynthesis of endogenous compounds and catabolism of xenobiotics, and their activities rely on a partner enzyme, cytochrome P450 reductase (CPR, E.C.1.6.2.4). Two CPR cDNAs, GhCPR1 and GhCPR2, were isolated from cotton (Gossypium hirsutum). They are 71% identical to each other at the amino acid sequence level and belong to the Class I and II of dicotyledonous CPRs, respectively. The recombinant enzymes reduced cytochrome c, ferricyanide and dichlorophenolindophenol (DCPIP) in an NADPH-dependent manner, and supported the activity of CYP73A25, a cinnamate 4-hydroxylase of cotton. Both GhCPR genes were widely expressed in cotton tissues, with a reduced expression level of GhCPR2 in the glandless cotton cultivar. Expression of GhCPR2, but not GhCPR1, was inducible by mechanical wounding and elicitation, indicating that the GhCPR2 is more related to defense reactions, including biosynthesis of secondary metabolites.
Yang
Chang-Qing
CQ
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China.
Lu
Shan
S
Mao
Ying-Bo
YB
Wang
Ling-Jian
LJ
Chen
Xiao-Ya
XY
D015086:2,6-Dichloroindophenol / Q000378:metabolism; D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D018076:DNA, Complementary; D005292:Ferricyanides / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins; D017386:Sequence Homology, Amino Acid; D050564:Trans-Cinnamate 4-Monooxygenase / Q000378:metabolism
D018076:DNA, Complementary; D005292:Ferricyanides; D010940:Plant Proteins; D011994:Recombinant Proteins; C007931:hexacyanoferrate III; D045304:Cytochromes c; D003577:Cytochrome P-450 Enzyme System; D015086:2,6-Dichloroindophenol; D050564:Trans-Cinnamate 4-Monooxygenase; C551015:CPRR1 protein, cotton; C551016:CPRR2 protein, cotton; D009251:NADPH-Ferrihemoprotein Reductase