Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis.
Chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans (GAGs) are present in high levels in connective tissue where they play roles as structural molecules and in protein-binding interactions. Recent developments in the techniques for analysis of CS/DS using capillary electrophoresis (CE) have enabled progress in the understanding of changes in CS/DS structure that accompany connective tissue diseases including osteoarthritis. Key to these developments is the ability to extract CS/DS GAGs from small quantities of connective tissue. This chapter describes a method for connective tissue GAG extraction, derivatization, and workup for subsequent capillary electrophoretic and/or mass spectrometric analysis.
Bielik
Alicia M
AM
Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
Zaia
Joseph
J
D000328:Adult; D002648:Child; D002807:Chondroitin / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003238:Connective Tissue / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D009844:Oligosaccharides / Q000737:chemistry; D010003:Osteoarthritis / Q000378:metabolism
D009844:Oligosaccharides; D003871:Dermatan Sulfate; D002807:Chondroitin; D002809:Chondroitin Sulfates