Genes expression of calcium signaling molecules in salivary glands of Drosophila melanogaster larvae.
Genes expression of the Itp-r-83A, Ca-P60A, olf186-F which encode inositol-1,4,5-trisphosphate receptor, endoplasmic reticulum Ca(2+)-ATPase and Oral protein--component of the store-operated Ca(2+)-entry respectively, was determined in salivary glands of Drosophila melanogaster larvae. For this purpose, the method of reverse transcription-polymerase chain reaction (RT-PCR) was used. Our results suggest that mentioned Ca(2+)-transport systems play an important role in maintaining of Ca(2+)-homeostasis in larval salivary glands of Drosophila melanogaster.
Chorna
T I
TI
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. tanya0104@yandex.ru
Hasan
G
G
Man'ko
V V
VV
Klevets
M Yu
MY
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000235:genetics*; D000252:Calcium-Transporting ATPases / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D017344:Genes, Insect; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics; D007814:Larva; D008565:Membrane Proteins / Q000235:genetics; D000071740:ORAI1 Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics; D012469:Salivary Glands / Q000378:metabolism*
D029721:Drosophila Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008565:Membrane Proteins; D000071740:ORAI1 Protein; D012269:Ribosomal Proteins; C107533:RpL32 protein, Drosophila; C510747:olf186-F protein, Drosophila; D000252:Calcium-Transporting ATPases; D002118:Calcium