title: Differential expression of liver proteins in Chianina and Holstein young bulls.
abstract: The purpose of this work was to produce and compare liver proteomic maps of cattle breeds that have been selected for different purposes: dairy (Holstein) and beef (Chianina). Liver was chosen because it displays functions of synthesis, homeostasis, excretion, and defense. Two dimensional electrophoresis analyses were performed on bovine livers collected from 4 individuals of each breed to evaluate differential protein expression. We found 9 spots differing in photodensity by >or=2-fold between the 2 breeds. Spots were analyzed through matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). A search against protein sequences in the National Center for Biotechnology Information databases indicated that the differentially expressed proteins correspond to liver-specific enzymes, with a major role in the metabolism of fatty acids, carbohydrates, AA, and the urea cycle.
authors.affiliation: Agricultural Research Council, Research Centre for Meat Production and Genetic Improvement, Via Salaria, 31 00016 Monterotondo, Rome, Italy. maria.miarelli@entecra.it
authors.forename: M
authors.initials: M
authors.lastname: Miarelli
authors.forename: F
authors.initials: F
authors.lastname: Signorelli
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D014176:Protein Biosynthesis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization