title: Differential expression proteomics of human colorectal cancer based on a syngeneic cellular model for the progression of adenoma to carcinoma.
abstract: This is the first differential expression proteomics study on a human syngeneic cellular in vitro progression model of the colorectal adenoma-to-carcinoma sequence, the anchorage-dependent non-tumorigenic adenoma derived cell line AA/C1 and the derived anchorage-independent and tumorigenic carcinoma cell line AA/C1/SB10C. The study is based on quantitative 2-DE and is complemented by Western blot validation. Excluding redundancies due to proteolysis and post-translational modified isoforms of over 2000 protein spots, 13 proteins were revealed as regulated with statistical variance being within the 95th confidence level and were identified by peptide mass fingerprinting in MALDI MS. Progression-associated proteins belong to the functional complexes of anaerobic glycolysis/gluconeogenesis, steroid biosynthesis, prostaglandin biosynthesis, the regulation and maintenance of the cytoskeleton, protein biosynthesis and degradation, the regulation of apoptosis or other functions. Partial but significant overlap was revealed with previous proteomics and transcriptomics studies in colorectal carcinoma. Among upregulated proteins we identified 3-HMG-CoA synthase, protein phosphatase 1, prostaglandin E synthase 2, villin 1, annexin A1, triosephosphate isomerase, phosphoserine aminotransferase 1, fumarylacetoacetate hydrolase and pyrroline-5-carboxylate reductase 1 (PYCR1), while glucose-regulated protein 78, cathepsin D, lamin A/C and quinolate phosphoribosyltransferase were downregulated.
authors.affiliation: Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Köln, Germany. Udo.Roth@qiagen.com
authors.forename: Udo
authors.initials: U
authors.lastname: Roth
authors.forename: Hanieh
authors.initials: H
authors.lastname: Razawi
authors.forename: Julia
authors.initials: J
authors.lastname: Hommer
authors.forename: Katja
authors.initials: K
authors.lastname: Engelmann
authors.forename: Tilo
authors.initials: T
authors.lastname: Schwientek
authors.forename: Stefan
authors.initials: S
authors.lastname: Müller
authors.forename: Stephan E
authors.initials: SE
authors.lastname: Baldus
authors.forename: Georgios
authors.initials: G
authors.lastname: Patsos
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Corfield
authors.forename: Christos
authors.initials: C
authors.lastname: Paraskeva
authors.forename: Franz-Georg
authors.initials: FG
authors.lastname: Hanisch
mesh_terms: D000236:Adenoma / Q000737:chemistry* / Q000473:pathology*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology*; D018450:Disease Progression; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008954:Models, Biological; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D020543:Proteome