title: Antistaphylococcal effect of enterocin AS-48 in bakery ingredients of vegetable origin, alone and in combination with selected antimicrobials.
abstract: The inhibitory effect of enterocin AS-48 against Staphylococcus aureus was investigated in various types of bakery ingredients. Antibacterial activity greatly depended on the food substrate, ranging from complete inactivation of S. aureus in liquid caramel (in which the bacterium survived poorly) to no significant inhibition (as in vanilla or chocolate creams). Significant reductions of viable counts in the range of 1.8 to 2.7 log units (P < 0.05) were achieved in substrates like pumpkin confiture or diluted almond cream stored at temperatures of 10 or 22 degrees C. Given the very low activity detected in chocolate substrates, enterocin AS-48 was tested in combination with other antimicrobials. Bactericidal activity increased markedly for the combinations of AS-48 and 0.1% eugenol (v/v), 0.5% 2-nitropropanol (v/v), or 3% Nisaplin (w/v). Enterocin AS-48 could be applied in combination with other antimicrobials for preservation of bakery ingredients against S. aureus.
authors.affiliation: Area de Microbiología, Depto de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ de Jaén, 23071-Jaén, Spain.
authors.forename: Pilar Martinez
authors.initials: PM
authors.lastname: Viedma
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.forename: Nabil Ben
authors.initials: NB
authors.lastname: Omar
authors.forename: Rosario Lucas
authors.initials: RL
authors.lastname: López
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D003080:Cold Temperature; D015169:Colony Count, Microbial; D004357:Drug Synergism; D005516:Food Microbiology; D005520:Food Preservatives / Q000494:pharmacology; D005517:Foodborne Diseases / Q000517:prevention & control; D008826:Microbial Sensitivity Tests / Q000379:methods; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D001430:Bacteriocins; D005520:Food Preservatives; C466236:enterocin AS-48, Enterococcus faecalis