title: Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton.
abstract: To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation System) indicate that these genes are involved in 68 biochemical pathways. These findings suggest that the related defense response, gossypol biosynthesis pathway and other complex regulation may be associated with pigment gland morphogenesis in cotton. The results may provide a basis for further study and serve as a guide for related research.
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, 400065, Chongqing, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Sun
authors.forename: Yingfan
authors.initials: Y
authors.lastname: Cai
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Xie
authors.forename: Jianchuan
authors.initials: J
authors.lastname: Mo
authors.forename: Youlu
authors.initials: Y
authors.lastname: Yuan
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Shi
authors.forename: Shengwei
authors.initials: S
authors.lastname: Li
authors.forename: Huaizhong
authors.initials: H
authors.lastname: Jiang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Pan
authors.forename: Yunling
authors.initials: Y
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: He
mesh_terms: D015536:Down-Regulation / Q000235:genetics; D017753:Ecosystem; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D003368:Gossypium / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant