[Effect of the mixed liquor of danshen and magnesium sulfate injection on IgG and IgM in serum of rabbits with lumbar intervertebral disc herniation].
OBJECTIVE
To explore the effect of the mixed liquor of danshen and magnesium sulfate injection on inflammatory reaction caused by autoimmune response of rabbits with lumbar intervertebral disc herniation.


METHODS
Sixty rabbits were divided into blank group (10 rabbits), sham operation group (10 rabbits), model group (40 rabbits) according to method of random digits table. Then model group was divided into made group (group A, 10 rabbits), normal saline group (group B, 10 rabbits), aescin natrium group (group C, 10 rabbits) and danshen and magnesium sulfate injection group (group D,10 rabbits). After model success of lumbar intervertebral disc herniation, different drugs were given to rabbits with lumbar intervertebral disc herniation by ear margin vein. The rabbits of the group C,B,D were respectively given aescin natriu (0.5 mg/kg), normal saline (5 ml/kg), danshen and magnesium sulfate injection (2.0 mg/kg). The rabbits of blank group, sham operation group and group A were not given any disposal. The IgG and IgM level in serum of different groups were determined with ELISA method in fourteen day after model success and drugs given.


RESULTS
The level of IgG and IgM in the group A was higher than that of blank group and sham operation group (P < 0.05). The level of IgG and IgM in the group D was lower than that of group B,C (P < 0.05).


CONCLUSIONS
The mixed liquor of danshen () and magnesium sulfate injection could inhibit inflammatory reaction caused by autoimmune response of lumbar intervertebral disc herniation,which provides a new alternative for lumbar intervertebral disc herniation.
Li
Zhi-bin
ZB
Department of Orthopaedics, the Affiliated Hospital of Shaanxi College of TCM, Xianyang 712000, Shaanxi, China.
Yuan
Pu-wei
PW
Zhu
Chao
C
D000818:Animals; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007267:Injections; D007405:Intervertebral Disc Displacement / Q000097:blood / Q000188:drug therapy* / Q000276:immunology*; D008278:Magnesium Sulfate / Q000008:administration & dosage*; D008297:Male; D010618:Phenanthrolines / Q000008:administration & dosage*; D011817:Rabbits; D011897:Random Allocation; D027561:Salvia miltiorrhiza / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010618:Phenanthrolines; C028972:dan-shen root extract; D008278:Magnesium Sulfate