Propionate and butyrate dependent bacterial sulfate reduction at extremely haloalkaline conditions and description of Desulfobotulus alkaliphilus sp. nov.
Evidence on the utilization of simple fatty acids by sulfate-reducing bacteria (SRB) at extremely haloalkaline conditions are practically absent, except for a single case of syntrophy by Desulfonatronum on acetate. Our experiments with sediments from soda lakes of Kulunda Steppe (Altai, Russia) showed sulfide production with sulfate as electron acceptor and propionate and butyrate (but not acetate) as an electron donor at a pH 10-10.5 and a salinity 70-180 g l(-1). With propionate as substrate, a highly enriched sulfidogenic culture was obtained in which the main component was identified as a novel representative of the family Syntrophobacteraceae. With butyrate as substrate, a pure SRB culture was isolated which oxidized butyrate and some higher fatty acids incompletely to acetate. The strain represents the first haloalkaliphilic representative of the family Desulfobacteraceae and is described as Desulfobotulus alkaliphilus sp. nov.
Sorokin
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Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia. soroc@inmi.host.ru
Detkova
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Muyzer
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D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism* / Q000494:pharmacology; D020564:Deltaproteobacteria / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011422:Propionates / Q000378:metabolism* / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012800:Siberia; D013431:Sulfates / Q000378:metabolism* / Q000494:pharmacology; D014871:Water Microbiology
D002087:Butyrates; D011422:Propionates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates