title: An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers.
abstract: Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.
authors.affiliation: Hamza Polat Vocational School, Ataturk University, Ispir-Erzurum, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Erturk
authors.forename: S
authors.initials: S
authors.lastname: Ercisli
authors.forename: D
authors.initials: D
authors.lastname: Maghradze
authors.forename: E
authors.initials: E
authors.lastname: Orhan
authors.forename: G
authors.initials: G
authors.lastname: Agar
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D014644:Genetic Variation; D027861:Prunus / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers