Study of the safety of transplantation of cultured autologous human chondroblasts.
Study of tumorigenic activity of cultured human chondroblasts intended for autologous transplantations showed that subcutaneous injection of human chondroblast suspension in a dose of 10(7)cells/0.2 ml did not cause the development of tumors in C.B-17 SCID immunodeficient mice over 12 weeks. Subcutaneous formations of compact cartilaginous consistency persisted at the site of chondroblast transplantation in all animals; these formations gradually shrank. Subcutaneous injection of tumor cell suspension (A204 human embryonic sarcoma) to C.B-17 SCID mice under the same conditions caused 100% development of tumors (poorly differentiated sarcomas) by day 14.
Rzhaninova
A A
AA
Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia. rzhaninova@rm7.ru
Ermakova
N P
NP
Merkulova
I B
IB
Mishin
A I
AI
Arutjunian
I V
IV
Volkov
A V
AV
Mikhailova
L M
LM
Murashev
A N
AN
Goldshtein
D V
DV
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID