[TRPV1 UTR-3 polymorphism and susceptibility of childhood asthma of the Han Nationality in Beijing].
OBJECTIVE
To explore TRPV1 UTR-3 polymorphism and susceptibility of childhood asthma of Han Nationality in Beijing.


METHODS
177 asthmatics, 44 atopy, and 151 control children less than 14-years-old were enrolled in case-control study, and all subjects were investigated by ISSAC questionnaires. Dominant, recessive, co-dominant, over-dominant, and log additive model were used to do genotype analysis, and LD analysis and haplotypes of SNPs were tested by Haploview 4.1. Hardy-Weinberg equilibrium test, Person chis-square test, linkage disequilibrium analysis, and logistic analysis were performed by SAS 9.1 software to determine the association between polymorphisms of TRPV1 and susceptibility of childhood asthma.


RESULTS
Polymorphisms were found in rs402369, rs4790521, and rs4790522, Hardy-Weinberg P > 0.05. As to allele frequencies, frequency of SNP rs4790521 T/C in asthmatics were significantly increased (P < 0.05), no significant difference were found in MAF of rs402369 and rs4790522 (P > 0.05). Genotype analysis showed that rs4790521 C/C and rs4790522 A/C were associated with childhood asthma (P < 0.05), and odd ratios were 2.94 (1.32 - 6.53) and 0.588 (0.376 - 0.920) respectively. LD were found between rs4790521 and rs4790522, 3 haplotypes were built. Adjusted by age, gender, parent asthma history, and smoking exposure, logistic stepwise analysis showed that MAF of rs4790521, allozygote C/C of rs4790521, and haplotype C/C were associated with susceptibility to childhood asthma in Chinese Han Nationality in Beijing (P < 0.05).


CONCLUSIONS
TRPV1 UTR-3 polymorphisms could be associated with the susceptibility to childhood asthma of Han Nation a city in Beijing.
Wang
Qiang
Q
Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Bai
Xuetao
X
Xu
Donggang
D
Xu
Dongqun
D
Li
Hong
H
Fang
Jianlong
J
Zhu
He
H
Fu
Wenliang
W
Cai
Xin
X
Wang
Jinfeng
J
Jin
Zhe
Z
Wang
Qin
Q
Xu
Chunyu
C
Chang
Junrui
J
D020413:3' Untranslated Regions / Q000235:genetics*; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D050916:TRPV Cation Channels / Q000235:genetics*
D020413:3' Untranslated Regions; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human