title: Is the expression pattern of BD ProExC the same as Ki-67? A comparative analysis in cervical biopsies.
abstract: BD ProExC (ProExC) and Ki-67, both used as biomarkers for high-grade cervical intraepithelial neoplasia (HG CIN), yield discordant staining in some cervical biopsies. This study compared ProExC and Ki-67 expression in 197 cervical biopsies with consensus diagnoses (55 negative, 21 atypical squamous metaplasia, 61 low-grade CIN, and 60 HG CIN). Percentages of immunostained nuclei were evaluated by 2 pathologists yielding 68 (35%) cases with discordant ProExC/Ki-67 immunostaining for analysis. In 78% of cases the difference in staining involved <25% of lesional cells. This was noted across all morphologic diagnoses, being most frequent in HG CIN. Discordant staining involving >50% of lesional cells occurred in 22% of discrepant cases, being most frequent in atypical squamous metaplasia. Using staining in >50% of lesional nuclei as a positive result, positive/negative discordance occurred in 25 cases (13% of all cases) including 18% of HG CIN cases. Fourteen cases were ProExC+ (7 of which were strongly p16+) and 11 were Ki-67+ (6 of which were strongly p16+).
authors.affiliation: Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. walts@cshs.org
authors.forename: Ann E
authors.initials: AE
authors.lastname: Walts
authors.forename: Shikha
authors.initials: S
authors.lastname: Bose
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000472:pathogenicity / Q000502:physiology*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D001706:Biopsy; D018797:Cell Cycle Proteins / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D004250:DNA Topoisomerases, Type II / Q000276:immunology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D055088:Early Detection of Cancer; D000939:Epitopes / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D000939:Epitopes; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D004250:DNA Topoisomerases, Type II