Molecular cytogenetic analysis of feline leukemia virus insertions in cat lymphoid tumor cells.
This study was conducted to map the acquired proviral insertions in the chromosomal genome of feline lymphoid tumors induced by feline leukemia virus (FeLV). Chromosome specimens of the lymphoid tumor-derived cell lines and normal cat lymphocytes were subjected to fluorescence in situ hybridization and tyramide signal amplification, using an exogenous FeLV-A genome as a probe. Specific hybridization signals were detected only on the metaphase chromosomes of the tumor cells. Poisson's distribution-based statistics indicated that 6 chromosomal loci in each cell line showed FeLV integration. In the examination of metaphase chromosomes of FL-74, FT-1 and KO-1 cells, significant signals were detected on B2p15-p14, B2q11, D1p14, E1p14-p13, E1q12 and F2q16; A2p23-p22, B2p15-p14, B4p15-p14, D4q23-q24, E1p14-p13 and E2p13-p12; and A2p22, A3q22, B1p13, B1q13, D1p13 and D3p15-p14, respectively. Consistently, Southern blot hybridization using an FeLV LTR-U3 probe specific for exogenous FeLV revealed the presence of at least 6 copies of exogenous FeLV proviruses at different integration sites in each cell line. These results indicate that there may be common FeLV integration sites at least in A2p22 and B2p15-p14. The cytogenetic analysis used in this study can promptly screen FeLV insertions and provide tags for identifying the novel common integration site.
Fujino
Yasuhito
Y
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. afujino@mail.ecc.u-tokyo.ac.jp
Satoh
Hitoshi
H
Ohno
Koichi
K
Tsujimoto
Hajime
H
D000818:Animals; D015139:Blotting, Southern; D002415:Cats; D045744:Cell Line, Tumor; D002875:Chromosomes / Q000821:virology; D020732:Cytogenetic Analysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D005256:Leukemia Virus, Feline / Q000235:genetics*; D008223:Lymphoma / Q000821:virology*; D016254:Mutagenesis, Insertional; D011533:Proviruses / Q000235:genetics*; D016662:Virus Integration