title: Identification of viral microRNAs expressed in human sacral ganglia latently infected with herpes simplex virus 2.
abstract: Deep sequencing of small RNAs isolated from human sacral ganglia latently infected with herpes simplex virus 2 (HSV-2) was used to identify HSV-2 microRNAs (miRNAs) expressed during latent infection. This effort resulted in the identification of five distinct HSV-2 miRNA species, two of which, miR-H3/miR-I and miR-H4/miR-II, have been previously reported. Three novel HSV-2 miRNAs were also identified, and two of these, miR-H7 and miR-H9, are derived from the latency-associated transcript (LAT) and are located antisense to the viral transcript encoding transactivator ICP0. A third novel HSV-2 miRNA, miR-H10, is encoded within the unique long (U(L)) region of the genome, 3' to the U(L)15 open reading frame, and is presumably excised from a novel, latent HSV-2 transcript distinct from LAT.
authors.affiliation: Department of Molecular Genetics and Center for Virology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Umbach
authors.forename: Kening
authors.initials: K
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Tang
authors.forename: Philip R
authors.initials: PR
authors.lastname: Krause
authors.forename: Erik K
authors.initials: EK
authors.lastname: Mont
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Cohen
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Cullen
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D005727:Ganglia, Spinal / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006561:Herpes Simplex / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012445:Sacrococcygeal Region / Q000821:virology*; D017735:Virus Latency
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral