Gene expression profiling of metabolism-related genes between top round and loin muscle of Korean cattle (Hanwoo).
Using differential display reverse transcriptase polymerase chain reaction, we detected 11 differentially expressed genes between top round and loin muscle in Korean cattle (Hanwoo). In the loin muscle, the lightness (L*) value (P<0.01) and marbling fat content (P<0.01), which are important factors in determining meat quality, were higher than in top round muscle. Three of the 11 genes were validated as significant genes between two types of muscle by real-time polymerase chain reaction (P<0.05). To determine whether the three genes were associated with meat quality traits, a regression analysis was preformed. The result demonstrated that two genes (NADH dehydrogenase 2 and cytochrome oxidase III), which are involved in oxidative phosphorylation in mitochondria, were significantly correlated with marbling fat content in the loin muscle (P<0.01), while two genes were not significant with marbling fat content in top round muscle. No significant effects for two genes on other meat quality traits such as meat color (redness and yellowness value), Warner-Bratzler shear force, and water-holding capacity were detected in this study.
Kim
Nam-Kuk
NK
National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea.
Cho
Yong-Min
YM
Jung
Yu-Sung
YS
Kim
Geon-Seok
GS
Heo
Kang-Nyeong
KN
Lee
Seung-Hwan
SH
Lim
Dajeong
D
Cho
Soohyun
S
Park
Eung-Woo
EW
Yoon
Duhak
D
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000235:genetics; D020869:Gene Expression Profiling / Q000662:veterinary*; D007723:Korea; D008297:Male; D008460:Meat / Q000032:analysis*; D008931:Mitochondria, Muscle / Q000201:enzymology; D018482:Muscle, Skeletal / Q000737:chemistry*; D009245:NADH Dehydrogenase / Q000235:genetics; D010085:Oxidative Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV