title: Fenvalerate inhibits the growth of primary cultured rat preantral ovarian follicles.
abstract: Fenvalerate is a widely used synthetic pyrethroid insecticide and is reported to disrupt reproductive function in humans and animals. However, little is known about its influence on follicular development. In this study, rat preantral follicles were primary cultured to investigate the effects of fenvalerate on follicular survival rate, morphological change, steroid hormone levels and steroidogenesis related gene mRNA expression. Follicles were cultured with 0, 1, 5 and 25 micromol/L fenvalerate for 72 h. And then the morphous was assessed by conventional light microscopy, steroid hormones were measured by RIA, and the expressions of steroidogenic acute regulatory protein (StAR) and cytochrome P450 side-chain cleavage enzyme (P450scc) were monitored by real-time quantitative PCR analysis. Results showed that fenvalerate inhibited the augmentation of follicular diameters but did not have detectable effects on follicular survival rates. The level of steroid hormones, such as progesterone, testosterone and estradiol, was inhibited. The inhibition might be due to the decreased expression levels of StAR and P450scc. These results suggested that fenvalerate restrained the follicular growth, and inhibited steroidogenesis by reducing StAR and P450scc gene expression, which might further contribute to the fenvalerate-induced reproductive dysfunction.
authors.affiliation: Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Fei
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Qu
authors.forename: Xin-Liang
authors.initials: XL
authors.lastname: Ding
authors.forename: Kai
authors.initials: K
authors.lastname: Xue
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Song
authors.forename: Yan-Kai
authors.initials: YK
authors.lastname: Xia
authors.forename: Shou-Lin
authors.initials: SL
authors.lastname: Wang
authors.forename: Xin-Ru
authors.initials: XR
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D009570:Nitriles / Q000633:toxicity*; D038081:Organogenesis / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D012739:Gonadal Steroid Hormones; D007306:Insecticides; D009570:Nitriles; D010750:Phosphoproteins; D011722:Pyrethrins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D003577:Cytochrome P-450 Enzyme System; C017690:fenvalerate