title: TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins.
abstract: A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of (15)N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232-13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Palmer
mesh_terms: D015820:Cadherins / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: C112316:CDH8 protein, human; D015820:Cadherins; D009587:Nitrogen Isotopes; D011506:Proteins