Backbone resonance assignment of Staphylococcal Enterotoxin H.
The staphylococcal enterotoxin H (SEH; 217 aa, 25 kD) belongs to a family of superantigens that cause a massive immune response upon simultaneous binding to the T cell receptor (TCR) and the major histocompatibility complex class II. The SEH-TCR interaction is weak and amenable to studies using NMR methodology. Essentially, 2 mg of U{(2)H, (13)C,(15)N}-labeled SEH was used for the complete sequential backbone assignment of SEH at 900 MHz. The protein secondary structure inferred from the chemical shift index (C(alpha) and C(beta)) is in very good agreement with the secondary structure elements of the X-ray structure.
Saline
Maria
M
Swedish NMR Centre, University of Gothenburg, PO Box 465, 405 30 Gothenburg, Sweden.
Orekhov
Vladislav
V
Lindkvist-Petersson
Karin
K
Karlsson
B Göran
BG
D000595:Amino Acid Sequence; D004768:Enterotoxins / Q000737:chemistry* / Q000235:genetics; D048168:Escherichia coli K12; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics; D018089:Superantigens / Q000737:chemistry* / Q000235:genetics
D004768:Enterotoxins; D011994:Recombinant Proteins; C402489:Staphylococcal enterotoxin H; D018089:Superantigens