Consequences of cerebroventricular insulin injection on renal sodium handling in rats: effect of inhibition of central nitric oxide synthase.
In the present study, we investigated the effects of acute intracerebroventricular (icv) insulin administration on central mechanisms regulating urinary sodium excretion in simultaneously centrally NG-nitro-L-arginine methylester (L-NAME)-injected unanesthetized rats. Male Wistar-Hannover rats were randomly assigned to one of five groups: a) icv 0.15 M NaCl-injected rats (control, N = 10), b) icv dose-response (1.26, 12.6 and 126 ng/3 microL) insulin-injected rats (N = 10), c) rats icv injected with 60 microg L-NAME in combination with NaCl (N = 10) or d) with insulin (N = 10), and e) subcutaneously insulin-injected rats (N = 5). Centrally administered insulin produced an increase in urinary output of sodium (NaCl: 855.6 +/- 85.1 Delta%/min; 126 ng insulin: 2055 +/- 310.6 Delta%/min; P = 0.005) and potassium (NaCl: 460.4 +/- 100 Delta%/min; 126 ng insulin: 669.2 +/- 60.8 Delta%/min; P = 0.025). The urinary sodium excretion response to icv 126 ng insulin microinjection was significantly attenuated by combined administration of L-NAME (126 ng insulin: 1935 +/- 258.3 Delta%/min; L-NAME + 126 ng insulin: 582.3 +/- 69.6 Delta%/min; P = 0.01). Insulin-induced natriuresis occurred by increasing post-proximal sodium excretion, despite an unchanged glomerular filtration rate. Although the rationale for decreased urinary sodium excretion induced by combined icv L-NAME and insulin administration is unknown, it is tempting to suggest that perhaps one of the efferent signals triggered by insulin in the CNS may be nitrergic in nature.
Oliveira
P C
PC
Faculdade de Medicina, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
Michelotto
J B
JB
Zapparoli
A
A
Boer
P A
PA
Gontijo
J A R
JA
D000818:Animals; D001921:Brain / Q000201:enzymology*; D007276:Injections, Intraventricular; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008845:Microinjections; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D009318:Natriuresis / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester