title: Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis (2-DE).
abstract: Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).
authors.affiliation: Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland. gabi@microb.uni.wroc.pl
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bugla-Płoskońska
authors.forename: Bozena
authors.initials: B
authors.lastname: Futoma-Kołoch
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skwara
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Doroszkiewicz
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003902:Detergents