title: Requirement for the basic helix-loop-helix transcription factor Dec2 in initial TH2 lineage commitment.
abstract: How naive CD4(+) T cells commit to the T helper type 2 (T(H)2) lineage is poorly understood. Here we show that the basic helix-loop-helix transcription factor Dec2 was selectively expressed in T(H)2 cells. CD4(+) T cells from Dec2-deficient mice showed defective T(H)2 differentiation in vitro and in vivo in an asthma model and in response to challenge with a parasite antigen. Dec2 promoted expression of interleukin 4 (IL-4), IL-5 and IL-13 during early T(H)2 differentiation and directly bound to and activated transcription of genes encoding the transcription factors JunB and GATA-3. As GATA-3 induces Dec2 expression, our findings also indicate a feed-forward regulatory circuit during T(H)2 differentiation.
authors.affiliation: Department of Immunology, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Xuexian O
authors.initials: XO
authors.lastname: Yang
authors.forename: Pornpimon
authors.initials: P
authors.lastname: Angkasekwinai
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhu
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.forename: Zhiduo
authors.initials: Z
authors.lastname: Liu
authors.forename: Roza
authors.initials: R
authors.lastname: Nurieva
authors.forename: Xikui
authors.initials: X
authors.lastname: Liu
authors.forename: Yeonseok
authors.initials: Y
authors.lastname: Chung
authors.forename: Seon Hee
authors.initials: SH
authors.lastname: Chang
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
authors.forename: Chen
authors.initials: C
authors.lastname: Dong
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D004195:Disease Models, Animal; D050990:GATA3 Transcription Factor / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism
chemical_list: C470968:Bhlhb3 protein, mouse; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors