title: The cross-reactivity of Shewanella fidelis lipopolysaccharide with anti-Proteus antibodies.
abstract: The serological cross-reactivity between lipopolysaccharides (LPS) of S. fidelis KMM3582(T) and rabbit anti-O P. mirabilis antibodies was tested. Using ELISA and Western blot cross-reactivity between S. fidelis LPS and antisera against P. mirabilis O14, O3 LPSs was found. The observed cross-reaction may suggest that anti-P. mirabilis S1959 (O3) antibodies may bind to the internal part of S. fidelis O-polysaccharides. A weak interaction between S. fidelis LPS and antiserum against P. mirabilis O13 in Western blot suggests that the absolute configuration of non-sugar "AlaLys" component (N(epsilon)-[(S)-l-carboxyethyl]-N(alpha)-(D-galacturonoyl)-L-lysine) may influence the affinity of antibodies for S. fidelis LPS.
authors.affiliation: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland. marek.kwinkowski@ujk.edu.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Kwinkowski
authors.forename: Sebastian
authors.initials: S
authors.lastname: Grabowski
authors.forename: Iwona
authors.initials: I
authors.lastname: Konieczna
authors.forename: Evgeny L
authors.initials: EL
authors.lastname: Nazarenko
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D011511:Proteus / Q000276:immunology*; D011817:Rabbits; D020592:Shewanella / Q000378:metabolism*
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides