title: Human liver progenitor cell lines are readily established from non-tumorous tissue adjacent to hepatocellular carcinoma.
abstract: Non-tumorous liver tissue removed during surgery to resect hepatocellular carcinoma (HCC) is potentially a useful source of material from which cells, particularly liver progenitor/stem cells (LPCs), can be isolated to establish cell lines. The purpose of this study was to evaluate the applicability of the "plate-and-wait" method to derive LPCs from resections to remove HCC. Three independent non-tumorous liver samples from HCC resection and 3 samples from liver donors were used for LPC isolation. Staining for LPC markers, OV6, CK19, and EpCAM, in the above liver samples demonstrated staining in only 2 of the non-tumorous samples. We isolated 2 human liver epithelial cell lines (HLECs) from these 2 samples. These HLECs were positive for general stem cell markers CD133, EpCAM, and Oct4. They expressed the liver progenitor cell markers OV6, CK14, and M2PK but not CK19. They also expressed the hepatocellular markers albumin, CK8, CK18, HNF4-alpha, and the drug-metabolizing gene CYP3A4. These cells accumulated glycogen, indocyanine green, and synthesized urea. They produced colonies in soft agar that showed anchorage-independent growth and their tumorigenic status was confirmed when they produced tumors following transfer to athymic nude mice. In contrast, the third non-tumorous tissue and 3 normal liver samples did not produce cell lines. This study establishes a correlation between the presence of LPCs in the source liver tissue and the ability to derive cell lines from these tissues. The phenotypic similarities between the LPCs and the HLECs suggest that a precursor-product relationship may exist between the 2 cell types.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Aibin
authors.initials: A
authors.lastname: Zhang
authors.forename: Roslyn
authors.initials: R
authors.lastname: London
authors.forename: Frederik Michael
authors.initials: FM
authors.lastname: Schulz
authors.forename: Philippe Wilfred Roger
authors.initials: PW
authors.lastname: Giguère-Simmonds
authors.forename: Luc
authors.initials: L
authors.lastname: Delriviere
authors.forename: Harsha
authors.initials: H
authors.lastname: Chandraratana
authors.forename: Kathy
authors.initials: K
authors.lastname: Hardy
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
authors.forename: John K
authors.initials: JK
authors.lastname: Olynyk
authors.forename: George
authors.initials: G
authors.lastname: Yeoh
mesh_terms: D000071916:AC133 Antigen; D053687:Adult Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002869:Chromosome Aberrations; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D000071858:Epithelial Cell Adhesion Molecule; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D006023:Glycoproteins / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010455:Peptides / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D006023:Glycoproteins; D053539:Keratin-19; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; C115011:oval cell marker OV-6; D014508:Urea; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human