Response of blood platelets to beta-glucan from Saccharomyces cerevisiae.
The effects of the beta-D-glucan, a polysaccharide derived from the yeast cell walls of species such as Saccharomyces cerevisiae, on blood platelets activation induced by physiological agonists (thrombin, ADP, collagen) in vitro were studied. The aim of our study was to assess in vitro if beta-glucan, a naturally strong biological response modifier, may modify platelet activation, i.e. platelet aggregation and degranulation (release of proteins and adenine nucleotides) induced by thrombin, ADP and collagen. Cytochrome c reduction method was used to test the ability of beta-glucan to change superoxide anion generation in platelets. Moreover, we determined also its effect on enzymatic arachidonic acid cascade. The obtained results indicate that beta-glucan has the inhibitory effects on platelet aggregation and secretion. beta-glucan distinctly reduced the arachidonic acid pathway and superoxide anion radical generation in platelets stimulated by biological agonists. The results of the present study suggest that beta-glucan from Saccharomyces cerevisiae has antiplatelet and antioxidative activities, and therefore may be beneficial in the prevention of the excessive blood platelet activation-related diseases, such as cardiovascular or inflammatory diseases.
Saluk-Juszczak
Joanna
J
Department of General Biochemistry, University of Lodz, Lodz, Poland. juszczak@biol.uni.lodz.pl
Królewska
Karolina
K
Wachowicz
Barbara
B
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D047071:beta-Glucans / Q000494:pharmacology*
D007155:Immunologic Factors; D017392:Thiobarbituric Acid Reactive Substances; D047071:beta-Glucans