High-throughput genetic mapping of mutants via quantitative single nucleotide polymorphism typing.
Advances in next-generation sequencing technology have facilitated the discovery of single nucleotide polymorphisms (SNPs). Sequenom-based SNP-typing assays were developed for 1359 maize SNPs identified via comparative next-generation transcriptomic sequencing. Approximately 75% of these SNPs were successfully converted into genetic markers that can be scored reliably and used to generate a SNP-based genetic map by genotyping recombinant inbred lines from the intermated B73 x Mo17 population. The quantitative nature of Sequenom-based SNP assays led to the development of a time- and cost-efficient strategy to genetically map mutants via quantitative bulked segregant analysis. This strategy was used to rapidly map the loci associated with several dozen recessive mutants. Because a mutant can be mapped using as few as eight multiplexed sets of SNP assays on a bulk of as few as 20 mutant F(2) individuals, this strategy is expected to be widely adopted for mapping in many species.
Liu
Sanzhen
S
Biology and the Genetics Institute, University of Florida, Gainesville, Florida 32610, USA.
Chen
Hsin D
HD
Makarevitch
Irina
I
Shirmer
Rebecca
R
Emrich
Scott J
SJ
Dietrich
Charles R
CR
Barbazuk
W Brad
WB
Springer
Nathan M
NM
Schnable
Patrick S
PS
D001947:Breeding; D002874:Chromosome Mapping / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D057166:High-Throughput Screening Assays / Q000379:methods*; D009154:Mutation; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D003313:Zea mays / Q000235:genetics
D005819:Genetic Markers