Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-polymer production from a local isolate, Brevibacillus invocatus MTCC 9039.
Accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] co-polymer by a local isolate, Brevibacillus invocatus MTCC 9039 under batch mode was investigated under glucose, acetate and propionate-supplemented conditions. Cells harvested at the stationary phase of growth depicted maximum accumulation of poly-3-hydroxybutyrate (PHB), i.e. 3% of dry cell weight (dcw) at pH 7.0 and temperature 30 degrees C at 48h of incubation. PHB accumulation reached up to 52% (dcw) under 3% glucose with 1% acetate supplementation. P(3HB-co-3HV) co-polymer synthesis was observed under propionate-supplemented condition, which reached up to 45% under 3% glucose with 1% propionate supplementation. Optimization of process parameters by response surface methodology (RSM) resulted into co-polymer accumulation up to 65% (dcw) at 2.08% glucose, 1.62% acetate, 0.75% propionate and 2.15 g l(-1) KH(2)PO(4) concentrations. This co-polymer exhibited comparable material properties with the commercial [P(3HB-co-3HV)] co-polymers, whereas the elasticity was tremendously high and could be comparable with polypropylene. Thus, B. invocatus MTCC 9039 is emerging as an interesting organism and could be exploited further for P(3HB-co-3HV) co-polymer production.
Sankhla
Indu Singh
IS
Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
Bhati
Ranjana
R
Singh
Akhilesh Kumar
AK
Mallick
Nirupama
N
D000085:Acetates / Q000737:chemistry; D056449:Bacillales / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry; D011422:Propionates / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D000085:Acetates; D002244:Carbon; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; D011126:Polypropylenes; D011422:Propionates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C029658:propionic acid