title: [Determination of the eliminating ratio of TCM polysaccharide to hydroxyl radical by kinetic fluorescent spectrophotometry].
abstract: OBJECTIVE
  To explore reaction dynamic of hydroxyl radical (*OH) and salicylic acid,and to determine the elimination ratios of TCM polysaccharide to *OH by kinetic fluorescent analysis.
  
  
  METHODS
  The impact dynamics factors of this reaction were studied by fluorescent, such as the reaction of concentration, reaction time and temperature. The dynamical equation was built, a kinetic fluorescent spectrophotometry based on the reaction was developed to determine the elimination ratio. Repetitiveness and reliability of this method were tested by vitamin C.
  
  
  RESULTS
  The dynamical equation of reaction rate to salicylic acid was gamma = 0. 9818x -1. 1801 under the condition of lambda ex = 295 nm, lambda em = 411 nm at room temperature, r approximately 1. The 50% elimination ratio (IC50) of TCM polysaccharide of Tangerine peel and Ganoderma lucidum to *OH was 78.01 mg/L and 232.5 mg/L, respectively. The IC50 of vitamin C was 24.52 microg/L, RSD was 0.23% (n = 5).
  
  
  CONCLUSIONS
  The method is sensitive and reliable, it can be used to determine the elimination ratio of TCM polysaccharide to *OH.
authors.affiliation: Department of Analytical Chemistry, Guangdong Pharmaceutical University, Guangzhou 510006, China. huipingf@126.com
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Feng
authors.forename: Yun-Yan
authors.initials: YY
authors.lastname: Mo
authors.forename: Min-Si
authors.initials: MS
authors.lastname: Huang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Pan
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Fan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers; D032902:Ganoderma / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020156:Salicylic Acid / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D017665:Hydroxyl Radical; D020156:Salicylic Acid