title: Important but little known (or forgotten) artifacts in protein biochemistry.
abstract: Many subtle and sometimes obscure artifacts exist that can have major effects on the outcomes of otherwise carefully performed experiments. This chapter focuses on a few of these artifacts involved in processes such as SDS gel sample preparation, buffers, Ni-chelate affinity and ion-exchange chromatography, urea preparation, use of plasticware, and filtration of protein samples.
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Burgess
mesh_terms: D000042:Absorption / Q000502:physiology; D000818:Animals; D016477:Artifacts; D001671:Biochemistry / Q000379:methods* / Q000592:standards; D002021:Buffers; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010969:Plastics / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D002021:Buffers; D010969:Plastics; D011506:Proteins