title: On-line combination of CE and microscopy: an insight into the migration of microorganisms.
abstract: Identification of microbial contamination by CE has interested many researchers mainly because of the high speed of CE analysis. However, the CE separation of such big structures brings a lot of questions mainly about the behavior of microorganisms and about the mechanism of separation. In this work, we constructed a simple apparatus where a microscope was used as one detector and a UV detector was used as the second one and we made the comparison of three typical setups for CE of microorganisms. Saccharomyces cerevisiae was chosen as a model microorganism and was analyzed in bare fused-silica capillaries, covalently modified capillaries and dynamically modified capillaries by poly(ethylene oxide) or CTAB. Results showed that the use of CE instrument directly connected with a microscope could be advantageous for the study of separation mechanisms and/or migration behavior.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic. secjpetr@gmail.com
authors.forename: Jan
authors.initials: J
authors.lastname: Petr
authors.forename: Olga
authors.initials: O
authors.lastname: Ryparová
authors.forename: Joanna
authors.initials: J
authors.lastname: Znaleziona
authors.forename: Vítĕzslav
authors.initials: V
authors.lastname: Maier
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevcík
mesh_terms: D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation*; D011092:Polyethylene Glycols / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D011092:Polyethylene Glycols