Development and validation of a simple and sensitive liquid chromatography-tandem mass spectrometry method for quantifying trimetazidine in human plasma.
1. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for quantifying trimetazidine in human plasma was developed and validated. Sample preparation was based on deproteinating with acetonitrile. 2. Chromatography was performed on a C18 analytical column (5 mum; 150 x 2.1 mm i.d.) and the retention times for trimetazidine and cetirizine (used as the internal standard) were 1.8 and 3.0 min, respectively. The ionization was optimized using an electrospray ionization source and enhanced selectivity was achieved using tandem mass spectrometry. The calibration curve ranged from 0.1 to 200 ng/mL. The inter-day precision, accuracy and the relative standard deviation (RSD) were all < 15%. The analyte was shown to be stable over the time-scale of the entire procedure. 3. The robustness of the method was demonstrated by the good reproducibility of the results obtained during the analysis of clinical samples.
Zhou
Gan
G
Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.
Tan
Zhi-Rong
ZR
Ouyang
Dong-Sheng
DS
Chen
Yao
Y
Guo
Dong
D
Xie
Hai-Tang
HT
Liu
Ying-Zi
YZ
Fan
Lan
L
Deng
Han-Wu
HW
D053000:Analytic Sample Preparation Methods; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D008832:Microchemistry / Q000379:methods; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014292:Trimetazidine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
D014292:Trimetazidine