Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis.
A water-soluble polysaccharide named CPS1 had been isolated from C. sinensis mycelium by hot water extraction, ethanol precipitation, anion-exchange, and gel-permeation chromatography. UV spectra, FTIR spectra, partial acid hydrolysis, PMP precolumn derivation, periodate oxidation and Smith degradation studies were conducted to elucidate its structure. The results indicated that CPS1 was a glucomannogalactan with the monosaccharide composition of glucose: mannose: galactose = 2.8: 2.9: 1. The total carbohydrate content of CPS1 was 99.0%. The weight-average molecular weight was 8.1 x 10(3) Da. The results predicted (1-->2) and (1-->4)-linkage of mannose, (1-->3)-linkage of galactose, (1--> ) and (1-->3, 6)-linkage of glucose composed the backbone of CPS1. CPS1 was also evaluated for its antioxidant activity in vitro, including scavenging effects on the hydroxyl radicals, the reducing power, Fe(2+)-chelating activity, scavenging effect on superoxide radicals, as well as the inhibition of hydrogen peroxide induced haemolysis. CPS1 showed a high antioxidant effect, especially scavenging effect of hydroxyl radicals, the reducing power and Fe(2+)-chelating activity. The results provide scientific support for the antioxidant activity and indicated a connection between antioxidant activity and reparation of renal failure.
Wang
Ying
Y
Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Jiangsu, China. wangying19830511@hotmail.com
Wang
Min
M
Ling
Yun
Y
Fan
Weiqiang
W
Wang
Yufeng
Y
Yin
Hongping
H
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000975:Antioxidants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D011134:Polysaccharides; D006861:Hydrogen Peroxide; D007501:Iron; D005947:Glucose; D008358:Mannose; D005690:Galactose