Expression profiling of a novel calcium-dependent protein kinase gene, LeCPK2, from tomato (Solanum lycopersicum) under heat and pathogen-related hormones.
A full-length cDNA LeCPK2 (GenBank GQ205414) from tomato (Solanum lycopersicum) encoding a calcium-dependent protein kinase (CDPK) was cloned by in silico cloning using NtCPK5 (AY971376) as a virtual probe. The deduced amino acid sequence of LeCPK2 contained the kinase, autoinhibitory, and calmodulin-like domains typical of CDPKs. Expression profiling indicated that LeCPK2 expressed predominantly in flowers and responded divergently to heat and cold stress, in which obvious mRNA accumulation was detected at 4 h under 42 degrees C stress, but no change in LeCPK2 mRNA levels was observed in 6 h at 4 degrees C. Mechanical wounding and phytohormones including ethylene, methyl jasmonate, and salicylic acid were also observed to arouse the expression of LeCPK2 in a similar pattern. mRNA accumulation was enhanced at 30 min and reached a maximum at 3 h, followed by a decrease to the normal level. All the results suggest that LeCPK2 is a novel versatile isoform of tomato CDPKs.
Chang
Wen-Jun
WJ
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Su
Huo-Sheng
HS
Li
Wei-Jing
WJ
Zhang
Zhi-Li
ZL
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000166:cytology / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D013314:Stress, Mechanical; D013312:Stress, Physiological / Q000235:genetics; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D010937:Plant Growth Regulators; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases