Differences in the expression of glycosaminoglycans in human fibroblasts derived from gingival overgrowths is related to TGF-beta up-regulation.
Glycosaminoglycans (GAGs) play important roles in cell behavior and have the ability to bind and modulate cytokines. Using primary cultured fibroblasts from hereditary gingival fibromatosis (HGF), normal gingiva (NG), and NG treated with cyclosporin-A (NGc) we show changes in the expression and structural characteristics of GAGs as well as in the expression of enzymes involved in their biosynthesis and degradation. In addition, we show the over-expression of TGF-beta1 and TGF-beta type II receptor in HGF and NGc. There is an increase in the GAGs retained in the cellular fraction, and the fine structure of galactosaminoglycans show a decrease in alpha-l-iduronic acid content in HGF and NGc. Elevated extracellular levels of low molecular weight hyaluronan (HA) are found in HGF due to increase in the expression of HA synthase 3 and hyaluronidases 1 and 2. The results bring new insights to the accumulation of extracellular matrix related to TGF-beta over-expression.
Dreyfuss
Juliana L
JL
Disciplina de Biologia Molecular, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
Veiga
Silvio S
SS
Coulson-Thomas
Vivien J
VJ
Santos
Isabel A
IA
Toma
Leny
L
Coletta
Ricardo D
RD
Nader
Helena B
HB
D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D005351:Fibromatosis, Gingival / Q000378:metabolism; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism; D019214:Gingival Overgrowth / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D006025:Glycosaminoglycans; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D016572:Cyclosporine