title: Single-nucleotide polymorphism of CD36 locus and obesity in European adolescents.
abstract: CD36 is a membrane receptor with a wide variety of functions, including the regulation of energy metabolism, fat storage, and adipocyte differentiation. To assess the relationship between CD36 gene single-nucleotide polymorphisms (SNPs) and obesity in adolescents, we evaluated the relationship between CD36 SNPs and the risk of obesity in a case-control study composed of 307 obese (age = 15.0 +/- 1.1 years) and 339 normal-weight adolescents (age = 14.6 +/- 1.1 years). To validate the results, we assessed the relation between the same SNPs and percentage of body fat (BF%) and BMI in 1,151 European adolescents (age = 14.8 +/- 1.4 years). SNPs with a minor allele frequency >0.10 were selected to tag CD36. Genotyping was performed on an Illumina system. Four SNPs (rs3211867, rs3211883, rs3211908, and rs1527483) were associated with increased risk of obesity in the case-control study (odds ratio (OR) (95% confidence interval)): 1.96 (1.26-3.04], P = 0.003; 1.73 (1.16-2.59), P = 0.007; 2.42 (1.47-4.01), P = 0.0005 and 1.95 (1.25-3.05), P = 0.003, respectively). The same four SNPs were associated with higher BMI (P < 0.05) and BF% (P < 0.04) in the validation study. Further analyses identified a haplotype (frequency: 0.05) carrying the minor allele of these SNPs as being associated with obesity (OR: 2.28; P = 0.0008) in the case-control study and with excess adiposity (i.e., higher BF% (P = 0.03) and BMI (P = 0.04)) in the validation study. Our data suggest that genetic variability at the CD36 gene locus could be associated with body weight variability in European adolescents but these findings require replication.
authors.affiliation: Department of Epidemiology and Public Health, Institut Pasteur de Lille, INSERM, U744, Université Lille Nord de France, UDSL, Lille, France.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Bokor
authors.forename: Vanessa
authors.initials: V
authors.lastname: Legry
authors.forename: Aline
authors.initials: A
authors.lastname: Meirhaeghe
authors.forename: Jonatan R
authors.initials: JR
authors.lastname: Ruiz
authors.forename: Beatrice
authors.initials: B
authors.lastname: Mauro
authors.forename: Kurt
authors.initials: K
authors.lastname: Widhalm
authors.forename: Yannis
authors.initials: Y
authors.lastname: Manios
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Luis A
authors.initials: LA
authors.lastname: Moreno
authors.forename: Dènes
authors.initials: D
authors.lastname: Molnàr
authors.forename: Jean
authors.initials: J
authors.lastname: Dallongeville
mesh_terms: D000293:Adolescent; D015992:Body Mass Index; D001835:Body Weight / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics*; D016022:Case-Control Studies; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D009765:Obesity / Q000453:epidemiology* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044465:White People / Q000235:genetics* / Q000706:statistics & numerical data*
chemical_list: D018955:CD36 Antigens