title: Bioaccumulation of amylose-like glycans by Helicobacter pylori.
abstract: BACKGROUND
  Helicobacter pylori cell surface is composed of lipopolysaccharides (LPSs) yielding structures homologous to mammalian Lewis O-chains blood group antigens. These structures are key mediators in the definition of host-microbial interactions and known to change their expression pattern in response to environmental pressure.
  
  
  OBJECTIVE
  The present work is focused on the identification of new H. pylori cell-surface glycosides. Special attention is further devoted to provide insights on the impact of in vitro subcultivation on H. pylori cell-surface phenotypes.
  
  
  METHODS
  Cell-surface glycans from H. pylori NCTC 11637 and two clinical isolates were recovered from the aqueous phase resulting from phenol:water extraction of intact bacteria. They were evaluated in relation to their sugars and glycosidic-linkages composition by CG-MS, size-exclusion chromatography, NMR, and Mass Spectrometry. H. pylori glycan profile was also monitored during subcultivation in vitro in agar and F12 liquid medium.
  
  
  RESULTS
  All three studied strains produce LPS expressing Lewis epitopes and express bioaccumulate amylose-like glycans. Bioaccumulation of amylose was found to be enhanced with the subcultivation of the bacterium on agar medium and accompanied by a decrease in the expression of LPS O-chains. In contrast, during exponential growth in F12 liquid medium, an opposite behavior is observed, that is, there is an increase in the overall amount of LPS and decrease in amylose content.
  
  
  CONCLUSIONS
  This work shows that under specific environmental conditions, H. pylori expresses a phase-variable cell-surface alpha-(1-->4)-glucose moiety.
authors.affiliation: Departamento de Química da Universidade de Aveiro, Campus de Santiago, Aveiro, Portugal.
authors.forename: José A
authors.initials: JA
authors.lastname: Ferreira
authors.forename: Cristiana
authors.initials: C
authors.lastname: Pires
authors.forename: Marina
authors.initials: M
authors.lastname: Paulo
authors.forename: Nuno F
authors.initials: NF
authors.lastname: Azevedo
authors.forename: M Rosário
authors.initials: MR
authors.lastname: Domingues
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Vieira
authors.forename: Mario A
authors.initials: MA
authors.lastname: Monteiro
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Coimbra
mesh_terms: D000688:Amylose / Q000737:chemistry / Q000378:metabolism*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D011134:Polysaccharides; D000688:Amylose