title: Stimulation of glioma cell motility by expression, proteolysis, and release of the L1 neural cell recognition molecule.
abstract: BACKGROUND
  Malignant glioma cells are particularly motile and can travel diffusely through the brain parenchyma, apparently without following anatomical structures to guide their migration. The neural adhesion/recognition protein L1 (L1CAM; CD171) has been implicated in contributing to stimulation of motility and metastasis of several non-neural cancer types. We explored the expression and function of L1 protein as a stimulator of glioma cell motility using human high-grade glioma surgical specimens and established rat and human glioma cell lines.
  
  
  RESULTS
  L1 protein expression was found in 17 out of 18 human high-grade glioma surgical specimens by western blotting. L1 mRNA was found to be present in human U-87/LacZ and rat C6 and 9L glioma cell lines. The glioma cell lines were negative for surface full length L1 by flow cytometry and high resolution immunocytochemistry of live cells. However, fixed and permeablized cells exhibited positive staining as numerous intracellular puncta. Western blots of cell line extracts revealed L1 proteolysis into a large soluble ectodomain (~180 kDa) and a smaller transmembrane proteolytic fragment (~32 kDa). Exosomal vesicles released by the glioma cell lines were purified and contained both full-length L1 and the proteolyzed transmembrane fragment. Glioma cell lines expressed L1-binding alphavbeta5 integrin cell surface receptors. Quantitative time-lapse analyses showed that motility was reduced significantly in glioma cell lines by 1) infection with an antisense-L1 retroviral vector and 2) L1 ectodomain-binding antibodies.
  
  
  CONCLUSIONS
  Our novel results support a model of autocrine/paracrine stimulation of cell motility in glioma cells by a cleaved L1 ectodomain and/or released exosomal vesicles containing L1. This mechanism could explain the diffuse migratory behavior of high-grade glioma cancer cells within the brain.
authors.affiliation: Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA. gracey@udel.edu
authors.forename: Muhua
authors.initials: M
authors.lastname: Yang
authors.forename: Shalini
authors.initials: S
authors.lastname: Adla
authors.forename: Murali K
authors.initials: MK
authors.lastname: Temburni
authors.forename: Vivek P
authors.initials: VP
authors.lastname: Patel
authors.forename: Errin L
authors.initials: EL
authors.lastname: Lagow
authors.forename: Owen A
authors.initials: OA
authors.lastname: Brady
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Magdy I
authors.initials: MI
authors.lastname: Boulos
authors.forename: Deni S
authors.initials: DS
authors.lastname: Galileo