[Apurinic/apyrimidinic sites (AP)--stress oxidative marker--in normal and neoplastic human endometrium].
OBJECTIVE
To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement.


METHODS
AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 noncancerous endometrial samples using Oxidative DNA Damage Kit Quantitative (Kamiya Biomedical Company).


RESULTS
Mean AP sites in noncancerous endometrium was 6.0 +/- 1.21 per 105 base pair. In EC group the mean AP sites level was greater than estimated in the reference group containing proliferative, secretory and hyperplastic endometrial samples (p < 0.05). There was no relation between AP sites and EC FIGO grading or the depth of the uterine wall neoplastic invasion.


CONCLUSIONS
In EC mean AP sites level is greater than estimated in noncancerous endometrium. AP sites are not a molecular marker of EC advancement.
Przadka-Rabaniuk
Dorota
D
II Klinika Połoznictwa i Ginekologii WUM w Warszawie.
Postawski
Krzysztof
K
D004249:DNA Damage; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D018384:Oxidative Stress; D014407:Tumor Cells, Cultured
D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase