[Blood examination].
Allergic blood examination such as radioallergosorbent test (RAST) is an important and sensitive method for detecting the allergen against allergic diseases including bronchial asthma, allergic rhinitis, atopic dermatitis, and food allergy. In this review, blood examination such as RAST and histamine release test (HRT) will be discussed. In 1967, Wide et al developed allergen detecting system such as RAST that measures the allergen specific IgE antibody. Now, several systems including capsulated hydrophilic carrier polymer (CAP)-RAST or multiple antigen simultaneous test (MAST) by using the fluoroenzyme immunoassay (FEIA) or other methods by using the ELISA are available. Another method for blood test is HRT that measures histamine release from the peripheral blood basophils after antigen addition in vitro. In general, HRT is thought to be more sensitive than RAST but available for only ten allergens. Also, 10-20% of patients are non-responder for this test.
Kato
Masahiko
M
Department of Allergy and Immunology, Gunma Children's Medical Center.
D000906:Antibodies / Q000097:blood*; D006636:Histamine Release / Q000502:physiology*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000097:blood*; D011852:Radioallergosorbent Test
D000906:Antibodies; D007073:Immunoglobulin E