Identification of a truncated cystatin SA-I as a saliva biomarker for oral squamous cell carcinoma using the SELDI ProteinChip platform.
New and more consistent biomarkers of oral squamous cell carcinoma (OSCC) are needed to improve early detection of the disease and to monitor patient management. The aim of this study was to detect new OSCC tumor markers in saliva. Unstimulated saliva, collected from patients with primary stage I OSCC as matched pre-and post-treatment samples, was used in the analysis. A surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF) ProteinChip system was used to screen for differentially expressed proteins in the saliva samples. This analysis revealed 26 proteins with significantly different expression levels in the pre-and post-treatment samples (P<0.05). A 14 kDa protein detected in pre-treatment saliva from the OSCC patients was identified as a truncated cystatin SA-I, with deletion of three amino acids from the N-terminus. The authors propose that ProteinChip analysis may provide a reliable screening test for early diagnosis of OSCC and that truncated cystatin SA-I might be a useful tumor biomarker for OSCC.
Shintani
S
S
Department of Oral & Maxillofacial Surgery, Showa University School of Dentistry, Tokyo, Japan. shintani@dent.showa-u.ac.jp
Hamakawa
H
H
Ueyama
Y
Y
Hatori
M
M
Toyoshima
T
T
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000601:surgery; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis*; D042241:Early Diagnosis; D005260:Female; D020869:Gene Expression Profiling; D005887:Gingival Neoplasms / Q000473:pathology / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology / Q000601:surgery; D009062:Mouth Neoplasms / Q000473:pathology* / Q000601:surgery; D009367:Neoplasm Staging; D040081:Protein Array Analysis; D040901:Proteomics; D012463:Saliva / Q000201:enzymology*; D055333:Salivary Cystatins / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D017384:Sequence Deletion; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014062:Tongue Neoplasms / Q000473:pathology / Q000601:surgery; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; D015853:Cysteine Proteinase Inhibitors; D055333:Salivary Cystatins; D012471:Salivary Proteins and Peptides