Study of the cytochrome b gene sequence in populations of Taiwan.
The cytochrome b gene (MTCYB) has been widely used in taxonomic research. In this study, the sequence polymorphism of the MTCYB gene was determined in 417 subjects of eight populations living in Taiwan (Taiwanese Han, indigenous Taiwanese, Tao, mainland Chinese, Filipino, Thai, Vietnamese, and Caucasian). Sequence variation from the revised Cambridge Reference Sequence and genetic distance between these populations were analyzed. There were 108 variable positions with a total of 99 haplotypes. Population-specific positions of MTCYB gene were noted in Tao and Caucasian populations. There were statistically significant differences of genetic distance between Taiwanese Han and Caucasian, between Taiwanese Han and Tao, and between Taiwanese Han and Filipino. A phylogenetic tree presents the genetic distances between these populations. In conclusion, there are sufficient sequence polymorphisms of the MTCYB gene in individuals of different populations, which may be used in the analyses of human ethnic groups in forensic casework.
Hwa
Hsiao-Lin
HL
Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University Hospital and National Taiwan University, No. 7, Chung-Shan South Road, Taipei 100, Taiwan.
Ko
Tsang-Ming
TM
Chen
Yao-Chang
YC
Chang
Yih-Yuan
YY
Tseng
Li-Hui
LH
Su
Yi-Ning
YN
Lee
James Chun-I
JC
D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D005006:Ethnicity / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044469:Racial Groups / Q000235:genetics; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D013624:Taiwan
D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b