[Study on the influence on the content of dehydrocavidine and total alkaloids in Corydalis saxicola by different drying methods].
OBJECTIVE
To evaluate the influence of five different drying methods on the contents of dehydrocavidine, a main active constituent, and total alkaloids in Corydalis saxicola.


METHODS
The whole plant samples of C. saxicola were harvested at its florescence stage, and then immediately divided into four parts of root, stem, leaf, and inflorescence. Each part of sample was dried by the following five methods, drying in a sunshine, drying in a shade, oven-drying at 60 degrees C, vacuum-drying at room temperature and frozen vacuum-drying, respectively. Then the contents of dehydrocavidine and total alkaloids were determined by RP-HPLC and ultraviolet spectrophotometry.


RESULTS
The contents of dehydrocavidine and total alkaloids in all the four parts of the sample processed by drying in a shade were significantly higher than those in the samples processed by the other four drying mehods.


CONCLUSIONS
Different drying methods could significantly influence the contents of dehydrocavidine and total alkaloids in C. saxicola. The process of drying in a shade for a long time would be the best.
Wang
Dong-Mei
DM
College of Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China. Isswdm@mail.sysu.edu.cn
Tang
Zhi
Z
He
Zhi-Chao
ZC
Cheng
Xuan-Xuan
XX
Wei
Zhong-Fu
ZF
Yang
De-Po
DP
Jiang
Lin
L
D000470:Alkaloids / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry*; D003890:Desiccation / Q000379:methods*; D005612:Freeze Drying; D010598:Pharmacognosy; D035261:Plant Components, Aerial / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000470:Alkaloids; D001600:Berberine Alkaloids; C011188:cavidine