Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter.
Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enhancement of antagonistic activities against Bacillus cereus. HPLC peaks of surfactin for recombinant fmbR-1 showed patterns of lipopeptides similar to those of the wild-type strain, and surfactin production of the recombinant fmbR-1 was up to about fivefold greater than that of the wild-type strain without induction by isopropyl beta--1-thiogalactopyranoside. However, the production of surfactin increased to about 10-fold more than that of the wild-type strain when it was induced by isopropyl beta--1-thiogalactopyranoside.
Sun
Huigang
H
Nanjing Agricultural University, Weigang, Nanjing Jiangsu, China.
Bie
Xiaomei
X
Lu
Fengxia
F
Lu
Yaping
Y
Wu
Yundailai
Y
Lu
Zhaoxin
Z
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D055666:Lipopeptides / Q000096:biosynthesis*; D009876:Operon; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic
D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide