title: Bradykinin enhances cell migration in human chondrosarcoma cells through BK receptor signaling pathways.
abstract: Bradykinin (BK) is an inflammatory mediator, and shows elevated levels in regions of severe injury and inflammatory diseases. BK has recently been shown to be involved in carcinogenesis and cancer progression. In this study, we found that BK increased the migration and the expression of alpha2beta1 integrin in human chondrosarcoma cells. We also found that human chondrosarcoma tissues had significantly higher expression of the B1 and B2 receptors comparing to normal cartilage. BK-mediated migration and integrin up-regulation was attenuated by B1 and B2 BK receptor siRNA or antagonist. Activations of phospholipase C (PLC), protein kinase Cdelta (PKCdelta), and NF-kappaB pathways after BK treatment was demonstrated, and BK-induced integrin expression and migration activity was inhibited by the specific inhibitor of PLC, PKCdelta, and NF-kappaB cascades. Taken together, our results indicated that BK enhances the migration of chondrosarcoma cells by increasing alpha2beta1 integrin expression through the BK receptors/PLC/PKCdelta/NF-kappaB signal transduction pathway.
authors.affiliation: Department of Orthopaedic Surgery, China Medical University Beigang Hospital, Yun-Lin County, Taiwan.
authors.forename: Wei-Hung
authors.initials: WH
authors.lastname: Yang
authors.forename: Jung-Tzu
authors.initials: JT
authors.lastname: Chang
authors.forename: Sheng-Feng
authors.initials: SF
authors.lastname: Hsu
authors.forename: Te-Mao
authors.initials: TM
authors.lastname: Li
authors.forename: Der-Yang
authors.initials: DY
authors.lastname: Cho
authors.forename: Chun-Yin
authors.initials: CY
authors.lastname: Huang
authors.forename: Yi-Chin
authors.initials: YC
authors.lastname: Fong
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Tang
mesh_terms: D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism*; D001920:Bradykinin / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002469:Cell Separation; D002813:Chondrosarcoma / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D038982:Integrin alpha2beta1 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D051745:Protein Kinase C-delta / Q000378:metabolism; D018002:Receptors, Bradykinin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D038982:Integrin alpha2beta1; D016328:NF-kappa B; D018002:Receptors, Bradykinin; D051745:Protein Kinase C-delta; D010738:Type C Phospholipases; D001920:Bradykinin