Screening of specific diagnostic peptides of swine hepatitis E virus.
BACKGROUND
Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. Elucidation of the major antigenic epitopes of swHEV is essential for the effective control of swHEV epidemics.


RESULTS
By bioinformatic analysis, we identified and then synthesized 12 peptides from open reading frames (ORFs) ORF1, ORF2 and ORF3, including swHEV-1 - swHEV-12. Using the results from ELISA, we selected swHEV-11 as the best candidate antigen and used it as a coating antigen for the development of peptide-based swine anti-HEV ELISA kits. The coefficient of variation (CV) the coefficient of variation (CV) varied between 4.3-7.2% in the same batch, and between 8.2-17.7% in six different batches. When comparing our swine peptide-based kit with the commercial recombinant-based kit, the humane anti-HEV IgG test had a 73.4% correspondence rate for them.


CONCLUSIONS
This is the first systemic study to screen the diagnostic peptides of swHEV and our findings strongly suggest that peptide swHEV-11 is a potent diagnostic reagent of swHEV that could be used in the development of highly efficient diagnostic assays for the specific and highly sensitive detection of anti-HEV activity in swine serum samples.
Zhao
Kai
K
Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China. kzhao118@yahoo.com.cn
Liu
Qiwen
Q
Yu
Ruisong
R
Li
Zhen
Z
Li
Jianyue
J
Zhu
Hong
H
Wu
Xiao
X
Tan
Furong
F
Wang
Jinbin
J
Tang
Xueming
X
D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018963:Hepatitis Antigens / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D016751:Hepatitis E / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D016752:Hepatitis E virus / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology
D018963:Hepatitis Antigens; D010455:Peptides