Use of a baculovirus-expressed structural protein for the detection of antibodies to foot-and-mouth disease virus type A by a blocking enzyme-linked immunosorbent assay.
A blocking enzyme-linked immunosorbent assay (ELISA) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with a cutoff of 53% inhibition. Its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking ELISA, indicating its potential as an alternative assay.
Ko
Young-Joon
YJ
National Veterinary Research and Quarantine Service, Anyang-6-dong, Anyang, Gyeonggi 430-824, South Korea. koyj@nvrqs.go.kr
Lee
Hyang-Sim
HS
Jeoung
Hye-Young
HY
Heo
Eun-Jeong
EJ
Ko
Hyo-Rim
HR
Chang
Byung-Sik
BS
Joo
Hoo-Don
HD
Gerelmaa
U
U
Dashzeveg
B
B
Tserendorj
S
S
Sodnomdarjaa
R
R
Park
Jong-Hyeon
JH
Kweon
Chang-Hee
CH
Cho
In-Soo
IS
Paik
Sang-Gi
SG
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D005822:Genetic Vectors; D006041:Goats; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins