Protective effect of stress-induced liver damage by saponin fraction from Codonopsis lanceolata.
Saponins are valuable principles found in various herbal medicine with pharmaceutical, cosmetical and nutraceutical merits. In this study, we evaluated the protective role of saponin fraction (Cl-SF), prepared from Codonopsis lanceolata, an ethnopharmacologically famous plant in Korea, China and Japan, on water immersion stress-induced liver damage and radical generation. Cl-SF clearly decreased the up-regulated levels of serum glutamate-oxalacetate transaminase and glutamate-pyruvate-transaminase induced by water-immersed stress conditions. Furthermore, Cl-SF seemed to block the stress-induced radicals. Thus, Griess and DPPH assays revealed that Cl-SF significantly suppressed both radical generation in sodium nitroprusside-treated RAW264.7 cells and nitric oxide production in LPS-treated RAW264.7 cells. Therefore, these results suggest that Cl-SF may be considered as a promising stress-regulatory principle with radical scavenging actions.
Kim
Min Ho
MH
School of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
Lee
Jaehwi
J
Yoo
Dae Sung
DS
Lee
Yong Gyu
YG
Byeon
Se Eun
SE
Hong
Eock Kee
EK
Cho
Jae Youl
JY
D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D029745:Codonopsis / Q000737:chemistry*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007101:Immersion; D008107:Liver Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010851:Picrates / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D013315:Stress, Psychological / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D017382:Reactive Oxygen Species; D012503:Saponins; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl