Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens.
In order to investigate the effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, production of reactive oxygen species (ROS) and lipid peroxidation in broiler chickens, 128 six-week-old broiler chickens were kept in a controlled-environment chamber. The broiler chickens were initially kept at 25 degrees C (relative humidity, RH, 70+/-5%) for 6d and subsequently exposed to 35 degrees C (RH, 70+/-5%) for 3h, then the heat stress was removed and the temperature returned to 25 degrees C (RH, 70+/-5%). Blood and liver samples were obtained before heat exposure and at 0 (at the end of the three-hour heating episode, this group is also abbreviated as the HT group), 1, 2, 4, 8, 12h after the stress was removed. The results showed that acute heat stress induced a significant production of ROS, function of the mitochondrial respiratory chain, antioxidative enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)] activity, and formation of malondialdehybe (MDA). Within the first 12h after removal of the heat stress, the acute modification of the above parameters induced by heat stress gradually approached to pre-heat levels. The results of the present study suggest that acute exposure to high temperatures may depress the activity of the mitochondrial respiratory chain. This leads to over-production of ROS, which ultimately results in lipid peroxidation and oxidative stress. When the high temperature was removed, the production of ROS, mitochondrial respiratory function and oxidative injury that were induced by acute heat exposure gradually approached the levels observed before heating, in a time-dependent manner.
Yang
Lin
L
Department of Animal Nutrition and Feed Science, College of Animal Science, South China Agriculture University, Guangzhou 510642, PR China.
Tan
Gao-Yi
GY
Fu
Yu-Qiang
YQ
Feng
Jin-Hai
JH
Zhang
Min-Hong
MH
D000704:Analysis of Variance; D000818:Animals; D002374:Catalase / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D002645:Chickens / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase