Monitoring the dissemination of the broad-host-range plasmid pB10 in sediment microcosms by quantitative PCR.
Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular design of quantitative PCR primers/probes where we took advantage of the mosaic aspect of the bacterial genomes to achieve a highly specific quantitative PCR detection system.
Bonot
Sébastien
S
Centre International de Recherche sur l'Eau et l'Environnement, Suez Environnement, Le Pecq, France.
Merlin
Christophe
C
D017931:DNA Primers / Q000235:genetics; D004783:Environmental Microbiology; D056186:Metagenomics / Q000379:methods*; D010957:Plasmids / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers