title: Effects of antibiotics on biochemical parameters, leukocytes and reactive oxygen species (ROS) in bitches after ovariectomy.
abstract: It is well known that following surgical procedures, a variety of patho-physiological alterations occurs in the host, such as changes in haemodynamic, endocrine and immune functions, as well as increase in Reactive Oxygen Species (ROS). Antibiotic administration, a common practice used in surgery, affects immune functions and ROS generation. Our study was aimed to investigate the effect of five different antibiotic regimens: amoxicillin, benzylpenicillin/dihydrostreptomycin, sulfametazine/sulfamerazine/ sulfathiazole, enrofloxacin, lincomycin/spectinomycin, administered twice (2 hours before skin incision and 6 hours after the end of the surgical suture), on biochemical parameters, leukocytes and ROS concentrations, in bitches undergoing open laparotomic ovariectomy. All treated bitches recovered from surgery without developing either systemic dysfunctions, or infections at the surgical site. Mean healing time was 7 +/- 3 days. An increase in white blood cell count, in differential leukocyte count and in ROS concentrations occurred 24 hours after the end of surgery, followed by a gradual decrease to basal values within the fourth day after surgery. The biochemical parameters remained quite constant throughout the study. In conclusion, our results show that the above antibiotic regimens, administered only twice, are efficient in inducing an uneventful recovery after laparotomic ovariectomy and preventing the development of infections in the bitch.
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine, University of Bari, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Rizzo
authors.forename: Marianna
authors.initials: M
authors.lastname: Pantaleo
authors.forename: Maddalena
authors.initials: M
authors.lastname: Mutinati
authors.forename: Carmelinda
authors.initials: C
authors.lastname: Trisolini
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Minoia
authors.forename: Massimo
authors.initials: M
authors.lastname: Spedicato
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Roscino
authors.forename: Stefano
authors.initials: S
authors.lastname: Punzi
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Pampurini
authors.forename: Felicita
authors.initials: F
authors.lastname: Jirillo
authors.forename: Raffaele Luigi
authors.initials: RL
authors.lastname: Sciorsci
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D019072:Antibiotic Prophylaxis / Q000379:methods; D004285:Dogs; D005260:Female; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D010052:Ovariectomy / Q000009:adverse effects; D017382:Reactive Oxygen Species / Q000097:blood*; D013530:Surgical Wound Infection / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D017382:Reactive Oxygen Species