Single-nucleotide polymorphism of CD36 locus and obesity in European adolescents.
CD36 is a membrane receptor with a wide variety of functions, including the regulation of energy metabolism, fat storage, and adipocyte differentiation. To assess the relationship between CD36 gene single-nucleotide polymorphisms (SNPs) and obesity in adolescents, we evaluated the relationship between CD36 SNPs and the risk of obesity in a case-control study composed of 307 obese (age = 15.0 +/- 1.1 years) and 339 normal-weight adolescents (age = 14.6 +/- 1.1 years). To validate the results, we assessed the relation between the same SNPs and percentage of body fat (BF%) and BMI in 1,151 European adolescents (age = 14.8 +/- 1.4 years). SNPs with a minor allele frequency >0.10 were selected to tag CD36. Genotyping was performed on an Illumina system. Four SNPs (rs3211867, rs3211883, rs3211908, and rs1527483) were associated with increased risk of obesity in the case-control study (odds ratio (OR) (95% confidence interval)): 1.96 (1.26-3.04], P = 0.003; 1.73 (1.16-2.59), P = 0.007; 2.42 (1.47-4.01), P = 0.0005 and 1.95 (1.25-3.05), P = 0.003, respectively). The same four SNPs were associated with higher BMI (P < 0.05) and BF% (P < 0.04) in the validation study. Further analyses identified a haplotype (frequency: 0.05) carrying the minor allele of these SNPs as being associated with obesity (OR: 2.28; P = 0.0008) in the case-control study and with excess adiposity (i.e., higher BF% (P = 0.03) and BMI (P = 0.04)) in the validation study. Our data suggest that genetic variability at the CD36 gene locus could be associated with body weight variability in European adolescents but these findings require replication.
Bokor
Szilvia
S
Department of Epidemiology and Public Health, Institut Pasteur de Lille, INSERM, U744, Université Lille Nord de France, UDSL, Lille, France.
Legry
Vanessa
V
Meirhaeghe
Aline
A
Ruiz
Jonatan R
JR
Mauro
Beatrice
B
Widhalm
Kurt
K
Manios
Yannis
Y
Amouyel
Philippe
P
Moreno
Luis A
LA
Molnàr
Dènes
D
Dallongeville
Jean
J
D000293:Adolescent; D015992:Body Mass Index; D001835:Body Weight / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics*; D016022:Case-Control Studies; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D009765:Obesity / Q000453:epidemiology* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044465:White People / Q000235:genetics* / Q000706:statistics & numerical data*
D018955:CD36 Antigens