The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells.
Matriptase is a type II transmembrane serine protease. In the present study, matriptase C-terminal fragments containing the catalytic serine protease domain were found to occur on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells transfected with a cDNA encoding the protease. This suggests that matriptase interacts with various potential substrates when expressed in simple epithelia.
Tsuzuki
Satoshi
S
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. tkmone@kais.kyoto-u.ac.jp
Miyake
Yuka
Y
Inouye
Kuniyo
K
Fushiki
Tohru
T
D000595:Amino Acid Sequence; D000818:Animals; D055162:Biocatalysis; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*
D010446:Peptide Fragments; D012697:Serine Endopeptidases; C485595:matriptase