title: Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood.
abstract: Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.
authors.affiliation: Department of Sleep Disorder Research (Alfressa), Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. tebisawa-tky@umin.net <tebisawa-tky@umin.net>
authors.forename: Takashi
authors.initials: T
authors.lastname: Ebisawa
authors.forename: Kahori
authors.initials: K
authors.lastname: Numazawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shimada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Izutsu
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Sasaki
authors.forename: Nobumasa
authors.initials: N
authors.lastname: Kato
authors.forename: Katsushi
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Honma
authors.forename: Sato
authors.initials: S
authors.lastname: Honma
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Shibata
mesh_terms: D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012893:Sleep Wake Disorders / Q000235:genetics* / Q000503:physiopathology*
chemical_list: D012333:RNA, Messenger; D056926:CLOCK Proteins