THE EFFECT OF dl-METHIONINE, l-CYSTINE, AND dl-ISOLEUCINE ON THE UTILIZATION OF PARENTERALLY ADMINISTERED DOG HEMOGLOBIN : A SUGGESTION FOR DESIGNING THE COMPOSITION OF THE "IDEAL" PROTEIN DIGEST.
1. Further observations on the utilization of parenterally administered dog hemoglobin show that oral supplements of dl-methionine and l-cystine improve the efficiency of utilization of hemoglobin N, while a fed supplement of dl-isoleucine alone is without effect. 2. When N-isoleucine is added to a fed supplement of methionine or methionine and cystine, the utilization of parenterally given hemoglobin N is even better than with the sulfur-containing amino acids alone. 3. A suggested approach to the problem of designing the quantitatively "ideal" amino acid mixture lies in the definition of what may be called total organism-amino acid patterns of rat, dog, man, etc. These may vary considerably not only at different developmental stages in a given species, but also certainly from one species to another. 4. Further attempts to detect globin in the peripheral circulation have pointed to the need for a highly specific procedure such as that an immunologic method may offer. 5. Reduced hemin in dog plasma migrates with alpha(1)-globulin and albumin in veronal buffer at pH 8.5 and the colored zones give strong hemochromogen absorption bands.
Miller
L L
LL
Departments of Pathology and Radiology, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
Alling
E L
EL
D000596:Amino Acids; D000818:Animals; D003553:Cystine; D006454:Hemoglobins; D006801:Humans; D007532:Isoleucine; D007930:Leucine; D008297:Male; D008715:Methionine; D011506:Proteins; D051381:Rats
D000596:Amino Acids; D006454:Hemoglobins; D011506:Proteins; D007532:Isoleucine; D003553:Cystine; D008715:Methionine; D007930:Leucine