title: The BCG1619c gene is not essential for invasion and intracellular persistence of Mycobacterium bovis BCG in human THP-1 and A549 cell lines.
abstract: The BCG1619c gene of Mycobacterium bovis bacillus Calmette-Guérin (BCG) encodes for a 24 kDa invasin-like protein and is identical to the Rv1566c gene of Mycobacterium tuberculosis. To assess whether this protein was necessary for entry and (or) intracellular persistence in professional phagocytes and (or) in lung epithelial cells, a BCG1619c knockout mutant of M. bovis BCG was generated and compared with the parental BCG strain for its ability to infect and multiply in human monocyte derived THP-1 cells and in the lung epithelial cell line A549. No significant difference between the mutated and the parental BCG strain was observed in either of these in vitro infection systems, indicating that the BCG1619c gene is not essential for cell invasion and intracellular growth of BCG.
authors.affiliation: Dipartimento di Patologia Sperimentale, Universita di Pisa, Italy. florio@biomed.unipi.it
authors.forename: Walter
authors.initials: W
authors.lastname: Florio
authors.forename: Franca Lisa
authors.initials: FL
authors.lastname: Brancatisano
authors.forename: Daria
authors.initials: D
authors.lastname: Bottai
authors.forename: Semih
authors.initials: S
authors.lastname: Esin
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Di Luca
authors.forename: Claudio
authors.initials: C
authors.lastname: Counoupas
authors.forename: Giuseppantonio
authors.initials: G
authors.lastname: Maisetta
authors.forename: Antonella
authors.initials: A
authors.lastname: Lupetti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Batoni
authors.forename: Mario
authors.initials: M
authors.lastname: Campa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D008168:Lung / Q000166:cytology; D009000:Monocytes / Q000382:microbiology*; D009154:Mutation; D009163:Mycobacterium bovis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins