Anti-complementary effect of polysaccharide B3-PS1 in Herba Scutellariae Barbatae (Scutellaria barbata).
The polysaccharide B3-PS1 was extracted and purified from Herba Scutellariae Barbatae (Scutellaria barbata D. Don), through bioactivity-guided fractionation. Average molecular weight of B3-PS1 was about 1,700,000 Da with a composition of Gal, Glc, Man and Ara in the ratio of 4.3:1.6:1.1:1.0, and trace of Rha, Fuc and Xyl. Preliminary data showed that 1) B3-PS1 inhibited complement activation of the classic pathway with CH(50) value of 0.12 +/- 0.02 mg/ml and of the alternative pathways with AP(50) value of 0.36 +/- 0.05 mg/ml; 2) B3-PS1 interacts with C1q, C1r, C1s, C2, C3, C4, C5 and C9 through a hemolytic assay. These results strongly suggested that B3-PS1 could be a potential candidate in treating those complement-associated diseases such as rheumatoid arthritis, Alzheimer's disease, and Adult Respiratory Distress Syndrome (ARDS).
Wu
Yan
Y
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, People's Republic of China.
Chen
Dao-Feng
DF
D000818:Animals; D003170:Complement Pathway, Alternative / Q000276:immunology*; D003171:Complement Pathway, Classical / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis / Q000276:immunology; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D031363:Scutellaria / Q000276:immunology*; D012756:Sheep
D010936:Plant Extracts; D011134:Polysaccharides; C524947:Scutellaria barbata extract