title: Development and validation of a simultaneous extraction procedure for HPLC-MS quantification of daptomycin, amikacin, gentamicin, and rifampicin in human plasma.
abstract: A simultaneous extraction method to measure daptomycin, amikacin, gentamicin, and rifampicin in human plasma, by high-performance liquid chromatography, was developed and validated. The method involved a rapid sample preparation by protein precipitation with acetonitrile followed by direct injection into a high-performance liquid chromatography system coupled with mass detection. Drug retention times were 10.00 +/- 0.25, 2.00 +/- 0.25, 3.50 +/- 0.25, 11.50 +/- 0.25, and 12.50 +/- 0.25 min for daptomycin, amikacin, gentamicin, rifampicin, and quinoxaline, respectively. Good linearity (mean r(2) = 0.998) was obtained for all drugs quantified over the range of clinically relevant concentrations in human plasma and the use of the internal standard quinoxaline improves accuracy (RSD% <14.9%) and intra-day (RSD% <11.56) and inter-day (RSD% <12.10) precision for the analytical procedure. The limits of quantification for daptomycin, amikacin, gentamicin, and rifampicin were 1.56, 2.34, 0.63, 0.63 microg/ml, respectively. Moreover, the addition of ion pair trifluoroacetic acid in the sample allowed the majority of gentamicin and amikacin separation. A rapid, specific, sensitive, accurate, and reproducible HPLC method was developed and validated to measure daptomycin, amikacin, gentamicin, and rifampicin in human plasma. This method is suitable for clinical pharmacokinetic studies.
authors.affiliation: Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera 164, Turin 10149, Italy. lorena.baietto@libero.it
authors.forename: Lorena
authors.initials: L
authors.lastname: Baietto
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Avolio
authors.forename: Francesco Giuseppe
authors.initials: FG
authors.lastname: De Rosa
authors.forename: Silvia
authors.initials: S
authors.lastname: Garazzino
authors.forename: Marianna
authors.initials: M
authors.lastname: Michelazzo
authors.forename: Giusi
authors.initials: G
authors.lastname: Ventimiglia
authors.forename: Marco
authors.initials: M
authors.lastname: Siccardi
authors.forename: Marco
authors.initials: M
authors.lastname: Simiele
authors.forename: Mauro
authors.initials: M
authors.lastname: Sciandra
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Perri
mesh_terms: D000583:Amikacin / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017576:Daptomycin / Q000097:blood*; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D012293:Rifampin / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000583:Amikacin; D017576:Daptomycin; D012293:Rifampin