Photo-click immobilization of carbohydrates on polymeric surfaces--a quick method to functionalize surfaces for biomolecular recognition studies.
Methods to rapidly functionalize specific polymeric surfaces with alkynes, which can subsequently be linked to azide-containing carbohydrates, are presented. The methods comprise two main concepts: azide photoligation and Cu-catalyzed azide-alkyne cycloaddition. 2-Azidoethyl-functionalized alpha-D-mannopyranoside was synthesized and covalently attached to alkyne-functionalized polymeric surfaces using the techniques. The protein recognition properties of the carbohydrate-presenting surfaces were evaluated using quartz crystal microbalance biosensor instrumentation.
Norberg
Oscar
O
Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, S-10044, Stockholm, Sweden.
Deng
Lingquan
L
Yan
Mingdi
M
Ramström
Olof
O
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D001672:Biocompatible Materials / Q000032:analysis*; D015374:Biosensing Techniques; D002241:Carbohydrates / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D008766:Methylmannosides / Q000737:chemistry; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D011791:Quartz; D013499:Surface Properties
D000480:Alkynes; D001386:Azides; D001672:Biocompatible Materials; D002241:Carbohydrates; D008766:Methylmannosides; D011108:Polymers; D011506:Proteins; D011791:Quartz; D003300:Copper