Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins.
Passage of an extract or supernatant fraction through a column of Affi-Gel Blue and batchwise elution can be a rapid and effective early procedure for removal of nucleic acid, concentration of the sample and purification of nucleotide binding proteins.
Deutscher
Murray P
MP
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida, USA.
D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D004396:Coloring Agents / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D009696:Nucleic Acids / Q000302:isolation & purification*; D009711:Nucleotides / Q000302:isolation & purification / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014227:Triazines / Q000494:pharmacology*
D004396:Coloring Agents; D004268:DNA-Binding Proteins; D009696:Nucleic Acids; D009711:Nucleotides; D016601:RNA-Binding Proteins; D014227:Triazines; C012842:Cibacron Blue F 3GA