Anti-CCR3 monoclonal antibody inhibits eosinophil infiltration in Angiostrongylus cantonensis-infected ICR mice.
ICR mice were each infected with 35 Angiostrongylus cantonensis larvae. One group of mice received an intraperitoneal injection of anti-CCR3 monoclonal antibody (mAb) (50 microg) at 10 days post-infection (dpi), while another similarly-treated group also received a booster injection (25 microg) at 12 dpi. All the mice were sacrificed at 14 dpi for pathological examination, enzyme-linked immunosorbent assay analysis and RNA extraction. The infiltration of eosinophils and the severity of eosinophilic meningitis were reduced in both the mAb-treated groups, relative to infected but untreated animals. The levels of CCL11 (eotaxin) in the peripheral circulation and the expression of the Th2-type cytokine interleukin-5 in the brains were significantly reduced. A. cantonensis infection is the major cause of eosinophilic meningoencephalitis in Taiwan, and the results of this study could be useful for the development of strategies to reduce the neurological damage caused by this infection.
Chuang
Chih-Cheng
CC
Section of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Su
Kua-Eyre
KE
Chen
Chi-Wu
CW
Fan
Chia-Kwung
CK
Lin
Feng-Kuan
FK
Chen
Yu-Shen
YS
Du
Wen-Yuan
WY
D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D054413:Chemokine CCL11 / Q000097:blood; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology*; D007155:Immunologic Factors / Q000008:administration & dosage / Q000276:immunology*; D007274:Injections, Intraperitoneal; D015848:Interleukin-5 / Q000096:biosynthesis; D008297:Male; D008581:Meningitis / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D051379:Mice; D008813:Mice, Inbred ICR; D054397:Receptors, CCR3 / Q000037:antagonists & inhibitors*; D017206:Strongylida Infections / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal; C515457:Ccl11 protein, mouse; C515351:Ccr3 protein, mouse; D054413:Chemokine CCL11; D007155:Immunologic Factors; D015848:Interleukin-5; D054397:Receptors, CCR3