A facile strategy for the modification of polyethylene substrates with non-fouling, bioactive poly(poly(ethylene glycol) methacrylate) brushes.
This manuscript reports a facile strategy for the modification of polyethylene, a widely used biomaterial, with a thin non-biofouling coating that can act as a platform to introduce biochemical cues to control and direct, e.g., cell adhesion and proliferation. The non-biofouling coating is produced, following a two-step strategy involving photobromination of the polyethylene substrate followed by surface-initiated ATRP of poly(ethylene glycol) methacrylate. The resulting coatings, which are referred to as polymer brushes, are robust and withstand prolonged exposure to aqueous ethanol and phosphate buffered saline and also do not show any signs of detachment or degradation in the in vivo studies over a period of 10 d. Functionalization of these polymer brush coatings with extracellular matrix derived peptide sequences, promotes adhesion and spreading of endothelial cells.
Lavanant
Laurent
L
Laboratoire des Polymères, Ecole Polytechnique Fédérale de Lausanne, Institut des Matériaux, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.
Pullin
Brian
B
Hubbell
Jeffrey A
JA
Klok
Harm-Anton
HA
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D054879:Halogenation; D008297:Male; D008689:Methacrylates / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods; D056951:Photoelectron Spectroscopy; D020959:Polyethylene / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011109:Polymethacrylic Acids; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D001672:Biocompatible Materials; D008689:Methacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; C528061:poly(poly(ethylene glycol)methacrylate); D011092:Polyethylene Glycols; D020959:Polyethylene