Isolation and characterization of a heteropolysaccharide from the corm of Amorphophallus campanulatus.
A water-soluble polysaccharide isolated from the aqueous extract of the corm of Amorphophallus campanulatus was found to contain D-galactose, D-glucose, 4-O-acyl-D-methyl galacturonate, and l-arabinose in a molar ratio 2:1:1:1. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This molecule showed splenocyte activation.
Das
Debsankar
D
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Mondal
Subhas
S
Roy
Sadhan K
SK
Maiti
Debabrata
D
Bhunia
Bibhas
B
Maiti
Tapas K
TK
Islam
Syed S
SS
D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017421:Sequence Analysis; D012995:Solubility; D013154:Spleen / Q000166:cytology
D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides