title: Carboxymethylated beta-glucan derived from Poria cocos with biological activities.
abstract: Water-insoluble beta-(1-3)-D-glucan isolated from the sclerotium of Poria cocos hardly exhibits biological activity. Therefore, it is advantageous to produce a value-added product from P. cocos. We extracted the beta-(1-3)-D-glucan from the sclerotium of P. cocos and synthesized a carboxymethylated derivative. The structural and physiological properties of the derivative were investigated. The carboxymethylation of the polysaccharides was confirmed by Fourier transform infrared spectroscopy, and the degree of substitution (DS) and molecular weight were obtained by the potentiometric titration and gel permeation chromatography (GPC) analysis, respectively. The carboxymethylation caused the enhancement of in vitro bile acid binding capacity of the polysaccharides, which would be explained by the improved water solubility and structural changes caused by carboxymethylation. In addition, in vitro antiradical capacity of the derivative was observed by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH).
authors.affiliation: Key Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, People's Republic of China. river0301@163.com
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Yu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Mao
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D008970:Molecular Weight; D055441:Poria / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D014867:Water; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001647:Bile Acids and Salts; D016166:Free Radical Scavengers; D047071:beta-Glucans; D014867:Water; C029933:carboxymethyl-beta-1,3-glucan