Capillary electrophoresis of human follicular fluid.
Some of the major serum proteins that are also found in follicular fluid, including transferrin, alpha-macroglobulin and albumin, are thought to play a role in oocyte maturation. This study set out to identify proteins in human follicular fluid by capillary zone electrophoresis and to investigate their relationship to follicular/oocyte maturity and fertility outcome. 176 individual follicular fluid samples, from 30 women undertaking in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI), were run using an optimized capillary zone electrophoresis method that gave a good separation of sixteen peaks in most samples. Nine of the peaks were identified and quantified but seven remain unknown and require further proteomic identification. Of the identified protein peaks, levels of each were corrected for follicular volume and total content calculated. No significant difference in protein levels was found with regard to oocyte recovery and fertilization. Protein concentrations tended to decrease as the follicular sphere increased whilst total content in follicular fluid increased in proportion to size. This is consistent with simple transudation across a sphere surface area which does not increase in proportion to the follicular fluid. This is not true of the concentration and content pattern of other proteins/biomolecules which are produced by follicular cells locally. In conclusion, neither concentration nor absolute levels of nine major proteins identified in follicular fluids correlated with oocyte presence and fertility outcome. Future work to remove more concentrated proteins (e.g. albumin) would enhance separation of smaller peaks and identification of the unknown molecules.
Wen
X
X
Biomedical Sciences, School of Health and Social Science, Middlesex University, Hendon NW44BT, UK.
Perrett
D
D
Patel
P
P
Li
Naijun
N
Docherty
S M
SM
Tozer
A J
AJ
Iles
R K
RK
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D004527:Egg Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010062:Ovulation Induction; D020554:Sperm Injections, Intracytoplasmic
D001798:Blood Proteins; D004527:Egg Proteins