Tagging for protein expression.
Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use.
Malhotra
Arun
A
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA.
D000345:Affinity Labels / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D015870:Gene Expression; D006801:Humans; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012995:Solubility
D000345:Affinity Labels; D011993:Recombinant Fusion Proteins