title: beta-Naphthoflavone protects mice from aristolochic acid-I-induced acute kidney injury in a CYP1A dependent mechanism.
abstract: OBJECTIVE
  The role of CYP1A in the protection of aristolochic acid (AA)I-induced nephrotoxicity has been suggested. In the present study we investigated the effects of beta-naphthoflavone (BNF), a non-carcinogen CYP1A inducer, on AAI-induced kidney injury.
  
  
  METHODS
  Mice were pretreated with 80 mg/kg BNF by daily intraperitoneal injection (ip) for 3 days followed by a single ip of 10 mg/kg AAI. AAI and its major metabolites in blood, liver and kidney, the expression of CYP1A1 and CYP1A2 in microsomes of liver and kidney, as well as the nephrotoxicity were evaluated.
  
  
  RESULTS
  BNF pretreatment prevented AAI-induced renal damage by facilitating the disposal of AAI in liver. BNF pretreatment induced the expression of CYP1A1 in both liver and kidney; but the induction of CYP1A2 was only observed in liver.
  
  
  CONCLUSIONS
  BNF prevents AAI-induced kidney toxicity primarily through CYP1A induction.
authors.affiliation: Centre for Drug Safety and Evaluation Research, State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
authors.forename: Xiang
authors.initials: X
authors.lastname: Xue
authors.forename: Yuan-feng
authors.initials: YF
authors.lastname: Wu
authors.forename: Guo-zheng
authors.initials: GZ
authors.lastname: Xin
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.forename: Tian-pei
authors.initials: TP
authors.lastname: Xie
authors.forename: Li-kun
authors.initials: LK
authors.lastname: Gong
authors.forename: Jin
authors.initials: J
authors.lastname: Ren
mesh_terms: D000208:Acute Disease; D000818:Animals; D034341:Aristolochic Acids / Q000378:metabolism / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2 / Q000187:drug effects / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D019324:beta-Naphthoflavone / Q000494:pharmacology*
chemical_list: D034341:Aristolochic Acids; D019324:beta-Naphthoflavone; C000228:aristolochic acid I; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2