title: [Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies specific to diphtheria toxin B subunit].
abstract: The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diphtheriae. For this purpose we have constructed the immune library of murine immunoglobulin genes. A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10(7) to 10(9) M(-1).
authors.forename: O S
authors.initials: OS
authors.lastname: Oliĭnyk
authors.forename: A A
authors.initials: AA
authors.lastname: Kaberniuk
authors.forename: T A
authors.initials: TA
authors.lastname: Redchuk
authors.forename: N V
authors.initials: NV
authors.lastname: Korotkevych
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Labyntsev
authors.forename: S I
authors.initials: SI
authors.lastname: Romaniuk
authors.forename: D V
authors.initials: DV
authors.lastname: Kolybo
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D004165:Diphtheria / Q000175:diagnosis / Q000276:immunology; D004167:Diphtheria Toxin / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D004167:Diphtheria Toxin; D019151:Peptide Library; D021122:Protein Subunits; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies