An improved glycerin-jelly mounting procedure for permanent preparations of helminth eggs.
Many attempts have been undertaken to make permanent preparations of helminth eggs. However, the resulting preparations either lacked durability or tended to deform thin-shelled eggs, such as those of the hookworm. To overcome these drawbacks, we have modified 2 aspects of the glycerin-jelly mounting procedure. First, we gradually changed the media in which the helminth eggs soaked, from 10% formalin via water to a 70% ethanol and 5% glycerin solution. It took 10 days, which is much longer than the time required for the processes previously reported. Second, we used a hole slide glass instead of a slide glass. Eggs of 11 species of helminths have been prepared with this procedure, and have kept their morphology without apparent change for more than 4 yr.
Kumagai
M
M
Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan. mkumagai@jikei.ac.jp
Inaba
T
T
Makioka
A
A
Ishiwata
K
K
Onishi
K
K
Watanabe
N
N
D000818:Animals; D005404:Fixatives; D005557:Formaldehyde; D005990:Glycerol; D006376:Helminths / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D010274:Parasitology / Q000379:methods*; D012997:Solvents
D005404:Fixatives; D012997:Solvents; D005557:Formaldehyde; D005990:Glycerol