Doppler optical microangiography improves the quantification of local fluid flow and shear stress within 3-D porous constructs.
Traditional phase-resolved Doppler optical coherence tomography (DOCT) has been reported to have potential for characterizing local fluid flow within a microporous scaffold. In this work, we apply Doppler optical microangiography (DOMAG), a new imaging technique developed by combining optical microangiography (OMAG) with a phase-resolved method, for improved assessment of local fluid flow and its derived parameters, shear stress, and interconnectivity, within highly scattering porous constructs. Compared with DOCT, we demonstrate a dramatic improvement of DOMAG in quantifying flow-related properties within scaffolds in situ for functional tissue engineering.
Jia
Yali
Y
An
Lin
L
Wang
Ruikang K
RK
D000792:Angiography / Q000379:methods*; D001158:Arteries / Q000033:anatomy & histology* / Q000502:physiology*; D021621:Imaging, Three-Dimensional / Q000379:methods; D055420:Perfusion Imaging / Q000379:methods*; D016062:Porosity; D033081:Shear Strength; D041623:Tomography, Optical Coherence / Q000379:methods*