Nanoparticles functionalized with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells.
Superparamagnetic iron oxide nanoparticles (SPIONs) can substantially improve the sensitivity of magnetic resonance imaging (MRI). We propose that SPIONs could be used to target and image cancer cells if functionalized with recombinant single chain Fv antibody fragments (scFv). We tested our hypothesis by generating antibody-functionalized (abf) SPIONs using a scFv specific for carcinoembryonic antigen (CEA), an oncofoetal cell surface protein. SPIONs of different hydrodynamic diameter and surface chemistry were investigated and targeting was confirmed by ELISA, cellular iron uptake, confocal laser scanning microscopy (CLSM) and MRI. Results demonstrated that abf-SPIONs bound specifically to CEA-expressing human tumour cells, generating selective image contrast on MRI. In addition, we observed that the cellular interaction of the abf-SPIONs was influenced by hydrodynamic size and surface coating. The results indicate that abf-SPIONs have potential for cancer-specific MRI.
Vigor
Kim L
KL
UCL Cancer Institute, Paul O'Gorman Building, 72 Huntley Street London WC1E 6DD, UK.
Kyrtatos
Panagiotis G
PG
Minogue
Shane
S
Al-Jamal
Khuloud T
KT
Kogelberg
Heide
H
Tolner
Berend
B
Kostarelos
Kostas
K
Begent
Richard H
RH
Pankhurst
Quentin A
QA
Lythgoe
Mark F
MF
Chester
Kerry A
KA
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology*; D003287:Contrast Media / Q000138:chemical synthesis*; D016503:Drug Delivery Systems / Q000379:methods; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies / Q000737:chemistry
D003287:Contrast Media; D005290:Ferric Compounds; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C000499:ferric oxide