[Transcriptional profiles of intestinal pig epithelial cell jejenum 2 cultures infected by vesicular stomatitis virus].
OBJECTIVE
To better understand the host inflammatory responses, in particular inflammatory cytokines responses of Vesicular Stomatitis virus (VSV) infection, and the host-VSV interaction.


METHODS
We used VSV Indian strain to infect the Intestinal Pig Epithelial Cell Jejenum (IPEC-J2) cell. Then we measured and analyzed the viral RNA by using real-time PCR. The transcript level of cytokines (IL-2, 6, 8, 10,12, IFN-alpha, IFN-gamma, TNF-alpha, TGF-beta and TLR3) were detected by real-time PCR.


RESULTS
We found that the transcript levels of IL-6, IL-8, IL-12, TNF-alpha and TLR3 were increased obviously whereas TGF-beta showed no significant difference. TPEC-J2 cell did not secrete IL-2, IL-10, IFN-alpha and IFN-gamma.


CONCLUSIONS
The level of inflammatory response was increased when VSV infected TPEC-J2 cell.
Wei
Zhanyong
Z
College of Animal Science and Veterinary Medicine, Henan Argricultural University, Zhengzhou, Henan 450002, China. weizhanyong@hotmail.com
Wang
Xuebin
X
Wang
Yabin
Y
Zhang
Hongying
H
Chen
Hongying
H
Yang
Mingfan
M
Cui
Baoan
B
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology; D015870:Gene Expression; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D014158:Transcription, Genetic; D054243:Vesicular Stomatitis / Q000235:genetics* / Q000276:immunology / Q000821:virology; D018116:Vesiculovirus / Q000235:genetics / Q000276:immunology*
D016207:Cytokines