title: An improved glycerin-jelly mounting procedure for permanent preparations of helminth eggs.
abstract: Many attempts have been undertaken to make permanent preparations of helminth eggs. However, the resulting preparations either lacked durability or tended to deform thin-shelled eggs, such as those of the hookworm. To overcome these drawbacks, we have modified 2 aspects of the glycerin-jelly mounting procedure. First, we gradually changed the media in which the helminth eggs soaked, from 10% formalin via water to a 70% ethanol and 5% glycerin solution. It took 10 days, which is much longer than the time required for the processes previously reported. Second, we used a hole slide glass instead of a slide glass. Eggs of 11 species of helminths have been prepared with this procedure, and have kept their morphology without apparent change for more than 4 yr.
authors.affiliation: Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan. mkumagai@jikei.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Kumagai
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: A
authors.initials: A
authors.lastname: Makioka
authors.forename: K
authors.initials: K
authors.lastname: Ishiwata
authors.forename: K
authors.initials: K
authors.lastname: Onishi
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D005404:Fixatives; D005557:Formaldehyde; D005990:Glycerol; D006376:Helminths / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D010274:Parasitology / Q000379:methods*; D012997:Solvents
chemical_list: D005404:Fixatives; D012997:Solvents; D005557:Formaldehyde; D005990:Glycerol