Protective effect of nicotine on oxidative and cell damage in rats with depression induced by olfactory bulbectomy.
We evaluated the effects of nicotine on cell and oxidative damage caused by olfactory bulbectomy (OBX). The rats were divided into seven groups as follows: i) control; ii) vehicle (6% ethanol); iii) treated with nicotine; iv) sham operated; v) olfactory bulbectomy (OBX); vi) OBX+vehicle; and vii) OBX+Nic. The OBX was performed using the trepanation of frontal bone. The olfactory bulbs were cut and removed without damage to the frontal cortex. Two weeks after surgery nicotine was administered chronically once daily for 14 days, intraperitoneally (i.p.) in doses of 1.5 mg/kg, two weeks after surgery. OBX caused an increase in lipid peroxidation products and caspase-3 but prompted a reduction in reduced glutathione (GSH) content and antioxidative enzyme activity. All these changes were reverted by treatment of nicotine (14 days). In conclusions: i) OBX induces oxidative stress and cell death by apoptosis; and ii) nicotine presents antidepressant and antioxidant effect. All these findings suggest that nicotine would be a therapeutic tool for depression, although more studies are needed in this area to define the appropriate treatment regime.
Túnez
Isaac
I
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Córdoba, Avda. Menendez Pidal s/n, 14004-Córdoba, Spain. fm2tufii@uco.es
Drucker-Colín
René
R
Montilla
Pedro
P
Peña
José
J
Jimena
Ignacio
I
Medina
Francisco Javier
FJ
Tasset
Inmaculada
I
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001007:Anxiety / Q000188:drug therapy; D001522:Behavior, Animal / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D003866:Depressive Disorder / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D009538:Nicotine / Q000494:pharmacology* / Q000627:therapeutic use; D009830:Olfactory Bulb / Q000601:surgery*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D015415:Biomarkers; D009538:Nicotine