Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation.
To study the role of different DNA repair genes in the resistance of Deinococcus radiodurans to mono- and polychromatic UV radiation, wild-type strain and knockout mutants in RecA, PprA, and IrrE of D. radiodurans were irradiated with UV-C (254 nm), UV-(A + B) (280-400 nm) and UV-A (315-400 nm) radiation, and survival was monitored. The strain deficient in recA was highly sensitive to UV-C radiation compared to the wild-type, but showed no loss of resistance against irradiation with UV-(A + B) and UV-A, while pprA and irrE-deficient strains exhibited elevated sensitivity to UV-A and UV-(A + B) radiation. These results suggest that the repair of DNA double-strand breaks is essential after treatment with highly energetic UV-C radiation, whereas protection from oxidative stress may play a greater role in resistance to environmentally relevant UV radiation.
Bauermeister
Anja
A
Institute of Aerospace Medicine, Radiation Biology Division, Research Group 'Astrobiology', German Aerospace Center (DLR), Linder Hoehe, 51147, Cologne, Germany.
Bentchikou
Esma
E
Moeller
Ralf
R
Rettberg
Petra
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D034301:Deinococcus / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D024881:Drug Resistance, Bacterial; D009154:Mutation; D018384:Oxidative Stress; D011938:Rec A Recombinases / Q000235:genetics / Q000502:physiology; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011938:Rec A Recombinases