title: Purification and characterization of neoagarotetraose from hydrolyzed agar.
abstract: Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinase of melanoma cells was strongly inhibited by neoagarotetraose at a concentration of 1 micron/ml and similarly inhibited at 10 and 100 microg/ml compared to those by arbutin or kojic acid. The activity of mushroom tyrosinase showed a 38% inhibition by neoagarotetraose at 1 microg/ml, and this inhibitory effect was more efficient than that by kojic acid. Neoagarotetraose revealed similar IC(50) (50% inhibition concentration) value for mushroom tyrosinase as that by kojic acid. These data suggest that neoagarotetraose generated from agar by recombinant beta-agarase might be a good candidate as a cosmetic additive for whitening effect.
authors.affiliation: Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University, Busan 617-736, Korea.
authors.forename: Ming-Kyung
authors.initials: MK
authors.lastname: Jang
authors.forename: Dong-Guen
authors.initials: DG
authors.lastname: Lee
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kim
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Yu
authors.forename: Hye-Ji
authors.initials: HJ
authors.lastname: Jang
authors.forename: Sueng Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Jang
authors.forename: Ye Ji
authors.initials: YJ
authors.lastname: Lee
authors.forename: Sang-Hyeon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000362:Agar / Q000737:chemistry*; D000363:Agaricales / Q000201:enzymology; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D005697:Galactosides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D006868:Hydrolysis; D008543:Melanins / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011485:Protein Binding; D011753:Pyrones / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D005697:Galactosides; D008543:Melanins; D009844:Oligosaccharides; D011753:Pyrones; C081798:neoagarotetraose; C011890:kojic acid; D000362:Agar; D014442:Monophenol Monooxygenase