title: Acute repair of chondrocytes in the rabbit tibiofemoral joint following blunt impact using P188 surfactant and a preliminary investigation of its long-term efficacy.
abstract: Recent studies have indicated that there may be a correlation between acute chondrocyte damage and joint degeneration reminiscent of early-stage osteoarthritis (OA). P188 surfactant has been shown to acutely restore the integrity of damaged chondrocytes; however, its long-term efficacy is unknown. The hypothesis of this study was that a single injection of P188 into a traumatized joint would acutely repair damaged cell membranes and maintain their viability in the long term. Twelve rabbits were divided into two groups, with and without P188, and sacrificed 4 days after tibiofemoral (TF) impact. Another six rabbits were sacrificed after 6 weeks and divided into two groups, with and without P188 treatment immediately posttrauma. Treatment with P188 increased the viable cell density 4 days posttrauma. A higher density of viable cells was also documented 6 weeks posttrauma in the treated versus untreated limb. The results of the current study confirm the acute efficacy of P188 treatment, and may suggest long-term efficacy of treatment, but additional studies are still needed to investigate the chronic implications of the acute repair of cells in the traumatized joint.
authors.affiliation: Orthopaedic Biomechanics Laboratories, College of Osteopathic Medicine, Michigan State University, A407 East Fee Hall, East Lansing, Michigan 48824, USA.
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Isaac
authors.forename: Nurit
authors.initials: N
authors.lastname: Golenberg
authors.forename: Roger C
authors.initials: RC
authors.lastname: Haut
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D020442:Poloxamer / Q000494:pharmacology*; D011817:Rabbits; D013264:Stifle / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D013501:Surface-Active Agents / Q000494:pharmacology*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014949:Wounds, Nonpenetrating / Q000188:drug therapy / Q000473:pathology
chemical_list: D013501:Surface-Active Agents; D020442:Poloxamer