Short communication: Methamphetamine treatment increases in vitro and in vivo HIV replication.
To delineate the mechanistic basis for the epidemiological association between methamphetamine use and accelerated progression to AIDS, we evaluated the direct in vitro and in vivo effects of methamphetamine on HIV-1 replication. Methamphetamine administration significantly increased HIV-1 production by both HIV-infected monocytes and CD4 T lymphocytes in vitro. In addition, in vivo methamphetamine treatment increased HIV production and viremia in mice transgenic for a replication-competent HIV provirus and human cyclin T1. Methamphetamine activated transcription of the HIV long terminal repeat (LTR) regulatory region, was associated with nuclear translocation of NF-kappaB. Our results provide further insights into the mechanisms by which methamphetamine accelerates disease course in HIV-infected individuals.
Toussi
Sima Shelly
SS
Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Joseph
Aviva
A
Zheng
Jian Hua
JH
Dutta
Monica
M
Santambrogio
Laura
L
Goldstein
Harris
H
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000821:virology; D002478:Cells, Cultured; D056906:Cyclin T / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D016325:HIV Long Terminal Repeat / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000821:virology; D008694:Methamphetamine / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D014766:Viremia / Q000188:drug therapy / Q000821:virology; D014779:Virus Replication / Q000187:drug effects*
D056906:Cyclin T; D008694:Methamphetamine