Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood.
Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.
Ebisawa
Takashi
T
Department of Sleep Disorder Research (Alfressa), Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. tebisawa-tky@umin.net <tebisawa-tky@umin.net>
Numazawa
Kahori
K
Shimada
Hiroko
H
Izutsu
Hiroyuki
H
Sasaki
Tsukasa
T
Kato
Nobumasa
N
Tokunaga
Katsushi
K
Mori
Akio
A
Honma
Ken-ichi
K
Honma
Sato
S
Shibata
Shigenobu
S
D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012893:Sleep Wake Disorders / Q000235:genetics* / Q000503:physiopathology*
D012333:RNA, Messenger; D056926:CLOCK Proteins