title: Differences in the virulence of two strains of Foot-and-Mouth Disease Virus Serotype A with the same spatiotemporal distribution.
abstract: During the 2000-2001 epidemic of Foot-and-Mouth Disease Virus (FMDV) in Argentina, two FMDV serotype A viruses were identified among others. Since different pathogenic properties between these virus strains were noticed in cattle, we evaluated several biological properties and features of FMDV A/Arg/00 and FMDV A/Arg/01 in order to compare these viruses in terms of virulence and pathogenicity. Our results indicate that FMDV A/Arg/00 grows less efficiently than FMDV A/Arg/01, exemplified by smaller sized plaques, retarded one-step growth curves and overall low viral yields. Also, FMDV A/Arg/00 displayed the lowest specific infectivity in suckling mice requiring 50-fold more infectious particles than FMDV A/Arg/01 to generate a LD50 in suckling mice. Finally, FMDV A/Arg/00 did not cause death in adult C57Bl/6 mice even at high doses (10(7)-10(6)PFU) whereas FMDV A/Arg/01 resulted lethal in doses as low as 10(2)PFU. Overall, we were able to demonstrate that these virus strains differ from each other in terms of virulence and pathogenicity.
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Cc25 B1712WAA, Castelar, Buenos Aires, Argentina.
authors.forename: Soledad
authors.initials: S
authors.lastname: García-Nuñez
authors.forename: Guido
authors.initials: G
authors.lastname: König
authors.forename: Analía
authors.initials: A
authors.lastname: Berinstein
authors.forename: Elisa
authors.initials: E
authors.lastname: Carrillo
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004195:Disease Models, Animal; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D012367:RNA, Viral / Q000032:analysis; D012703:Serotyping; D016019:Survival Analysis; D013997:Time Factors; D019562:Viral Load; D010948:Viral Plaque Assay; D014774:Virulence
chemical_list: D012367:RNA, Viral