Development of Gateway binary vectors, R4L1pGWBs, for promoter analysis in higher plants.
We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were beta-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).
Nakamura
Shinya
S
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.
Nakao
Akihide
A
Kawamukai
Makoto
M
Kimura
Tetsuya
T
Ishiguro
Sumie
S
Nakagawa
Tsuyoshi
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013997:Time Factors; D014170:Transformation, Genetic
D018076:DNA, Complementary