Effect of curcumin on lung resistance-related protein (LRP) in retinoblastoma cells.
OBJECTIVE
Multidrug resistance to anticancer drugs is a major cause of chemotherapy failure in cancer patients. Lung resistance-related protein (LRP) has been identified as the major vault protein (MVP) in humans, which is associated with multidrug resistance in various cancer cells. Overexpression of LRP is found in many solid tumors and cancer cell lines.


METHODS
In this study, curcumin mixture was used to study the modulation of LRP in Y79 retinoblastoma cell line by Western blot and RT-PCR.


RESULTS
RT-PCR and Western blot was carried to determine the expression of LRP mRNA and protein level. Western blot and RT-PCR analysis showed that curcumin inhibited LRP expression in a dose-dependent manner in the Y79 retinoblastoma cell line.


CONCLUSIONS
These results demonstrate that curcumin modulated the expression of LRP in the Y79 retinoblastoma cell line. Thus, curcumin may be used as a chemosensitizer to make the retinoblastoma cells more sensitive to the effects of chemotherapy.
Thiyagarajan
Seethalakshmi
S
L&T Department of Ocular Pathology, Vision Research Foundation, Chennai, India.
Thirumalai
Karthiyayini
K
Nirmala
Sudarshan
S
Biswas
Jyotirmay
J
Krishnakumar
Subramanian
S
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D015153:Blotting, Western; D002461:Cell Line, Transformed; D003474:Curcumin / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D019572:Retinal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D012175:Retinoblastoma / Q000188:drug therapy / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020394:Vault Ribonucleoprotein Particles / Q000096:biosynthesis* / Q000235:genetics; D000099187:Major Vault Protein
D000970:Antineoplastic Agents; D003474:Curcumin; D012333:RNA, Messenger; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein