Twins born after transplantation of ovarian cortical tissue and oocyte vitrification.
OBJECTIVE
To present a combination of ovarian tissue and oocyte cryopreservation as an effective strategy for achieving pregnancy in a breast cancer patient.


METHODS
Case report.


METHODS
Tertiary care university-affiliated hospital, tissue bank, and infertility clinic.


METHODS
A 36-year-old patient diagnosed with atypical medullar breast cancer and negative for estrogen, P, and HER2 receptors underwent ovarian tissue cryopreservation before receiving chemotherapy and radiotherapy.


METHODS
Laparoscopic ovarian cortex extraction, ovarian tissue cryopreservation, ovarian tissue thawing and transplantation, controlled ovarian stimulation (COS), oocyte retrieval, vitrification and IVF, and embryo culture and replacement.


METHODS
Resumption of spontaneous ovarian function after transplantation, response to COS, oocyte vitrification, IVF, pregnancy, and delivery.


RESULTS
Menses occurred 63 days after transplantation. Sixteen mature oocytes were obtained in four COS procedures. All vitrified oocytes survived warming, and 77.7% were fertilized. Two day 3 embryos were replaced, and two healthy boys were born at 34 weeks.


CONCLUSIONS
Ovarian tissue cryopreservation and grafting preserves fertility. Simultaneous oocyte vitrification increases the success of assisted reproductive technology in poor-prognosis patients and avoids the consequences of the short lifespan of the transplanted tissue.
Sánchez-Serrano
María
M
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Crespo
Juana
J
Mirabet
Vicente
V
Cobo
Ana C
AC
Escribá
María-José
MJ
Simón
Carlos
C
Pellicer
Antonio
A
D000328:Adult; D001943:Breast Neoplasms / Q000503:physiopathology / Q000628:therapy*; D015925:Cryopreservation; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes; D010053:Ovary / Q000637:transplantation*; D010062:Ovulation Induction; D011247:Pregnancy; D016896:Treatment Outcome; D014427:Twins