Protein pattern transfer for biosensor applications.
This paper presents a very simple, industrially scalable method for transferring a high-resolution, biologically active protein pattern from one substrate to another. We demonstrate the transfer of a protein pattern formed initially by microcontact printing from a silicon surface (to which this form of printing is applicable) onto a glass or polymer substrate, almost independently of the surface/bulk properties of the second substrate. A very thin, spin-coated layer of a sugar is used to preserve the structure and organization of proteins during the subsequent plasma deposition of a siloxane polymer, after which the protein pattern could simply be peeled off the silicon substrate and glued onto any other desired substrate.
Volcke
C
C
Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin, Ireland. cedric.volcke@fundp.ac.be
Gandhiraman
R P
RP
Basabe-Desmonts
L
L
Iacono
M
M
Gubala
V
V
Cecchet
F
F
Cafolla
A A
AA
Williams
D E
DE
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008422:Materials Testing; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D001672:Biocompatible Materials; D011506:Proteins