[Effects of naringin on proliferation, differentiation and matrix mineralization of MC3T3-E1 cells].
OBJECTIVE
To investigate the effect of naringin on the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells in vitro.


METHODS
MC3T3-E1 cell lines were taken in vitro model. CCk-8 method was used to observe the proliferation of MC3T3 cells. Lactic acid dehydrogenase cytotoxicity (LDH) test was used to observe the cell toxicity. Bone morphogenetic protein-2 (BMP-2), alkaline phosphatase (ALP) and osteocalcin (OC) were used to observe the cell differentiation. Von kossa calcification staining method was used to observe the cell calcification.


RESULTS
The high dosages of the naringin could promote the proliferation of MC3T3-E1 cells at both 12 h and 24 h. While, low dosages did not show the same capability. LDH test showed that the cytotoxicity percentages in all six naringin treated groups were quite low. BMP-2 cytoimmunochemistry test showed that the three naringin treated group (10, 1, 0.1 micromol x L(-1)) showed higher brown coloration in cytoplasm than the control group at both 24 h and 48 h. 1, 0.1 micromol x L(-1) naringin raised ALP activity of MC3T3-E1 cells at 48 h (P < 0.05). Meanwhile, 0.1 micromol x L(-1) naringin increased the ALP activity at 72 h (P < 0.05). 10 and 1 micromol x L(-1) naringin increased the capability of MC3T3-E1 cell to synthesize osteocalcin during 8th - 12th dsince adding the medicine (P < 0.05). Naringin did not show the positive effects on cell calcification.


CONCLUSIONS
Naringin could promote proliferation and differentiation of MC3T3-E1 cells.
Ding
Peihui
P
Department of Stomatology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. phding@163.com
Tang
Qi
Q
Chen
Lili
L
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism
D055396:Bone Morphogenetic Protein 2; D044950:Flavanones; D015675:Osteocalcin; C005274:naringin