Neuronal RNA granule contains ApCPEB1, a novel cytoplasmic polyadenylation element binding protein, in Aplysia sensory neuron.
The cytoplasmic polyadenylation element (CPE)-binding protein (CPEB) binds to CPE containing mRNAs on their 3' untranslated regions (3'UTRs). This RNA binding protein comes out many important tasks, especially in learning and memory, by modifying the translational efficiency of target mRNAs via poly (A) tailing. Overexpressed CPEB has been reported to induce the formation of stress granules (SGs), a sort of RNA granule in mammalian cell lines. RNA granule is considered to be a potentially important factor in learning and memory. However, there is no study about RNA granule in Aplysia. To examine whether an Aplysia CPEB, ApCPEB1, forms RNA granules, we overexpressed ApCPEB1-EGFP in Aplysia sensory neurons. Consistent with the localization of mammalian CPEB, overexpressed ApCPEB1 formed granular structures, and was colocalized with RNAs and another RNA binding protein, ApCPEB, showing that ApCPEB1 positive granules are RNA-protein complexes. In addition, ApCPEB1 has a high turnover rate in RNA granules which were mobile structures. Thus, our results indicate that overexpressed ApCPEB1 is incorporated into RNA granule which is a dynamic structure in Aplysia sensory neuron. We propose that ApCPEB1 granule might modulate translation, as other RNA granules do, and furthermore, influence memory.
Chae
Yeon-Su
YS
National Creative Research Initiative Center for Memory and Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Korea.
Lee
Seung-Hee
SH
Cheang
Ye-Hwang
YH
Lee
Nuribalhae
N
Rim
Young-Soo
YS
Jang
Deok-Jin
DJ
Kaang
Bong-Kiun
BK
D000818:Animals; D001048:Aplysia / Q000235:genetics / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D039221:mRNA Cleavage and Polyadenylation Factors; D012313:RNA