CD9 expression can be used to predict childhood TEL/AML1-positive acute lymphoblastic leukemia: proposal for an accelerated diagnostic flowchart.
CD9 has been shown to be differentially expressed in childhood TEL/AML1-positive acute lymphoblastic leukemia (ALL). We confirmed this finding in large Affymetrix data sets and in 80 new cases at both RNA and protein levels. Moreover, we showed that mean fluorescence intensity of CD9 by flow cytometry can distinguish TEL/AML1-positive ALL from other BCP-ALL. Using ROC analysis, the most efficient model for predicting TEL/AML1-positive ALL combined CD9 (mean fluorescence intensity <or=20) and CD10 values (positive cells >40%). Finally, we propose a faster procedure for optimizing the diagnosis of childhood BCP-ALL subgroups.
Gandemer
Virginie
V
Centre National de Recherche Scientifique, Unité Mixte de Recherche 6061 Laboratoire de Génétique et Développement, Equipe Régulation Transcriptionnelle et Oncogenèse, Université de Rennes-1, Rennes Cedex, France.
Aubry
Marc
M
Roussel
Mikael
M
Rio
Anne-Gaelle
AG
de Tayrac
Marie
M
Vallee
Audrey
A
Mosser
Jean
J
Ly-Sunnaram
Béatrice
B
Galibert
Marie-Dominique
MD
D000465:Algorithms; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002648:Child; D050676:Core Binding Factor Alpha 2 Subunit / Q000032:analysis / Q000235:genetics*; D003661:Decision Support Techniques; D005189:False Positive Reactions; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*; D011379:Prognosis; D060245:Tetraspanin 29; D013997:Time Factors; D014407:Tumor Cells, Cultured; D000097764:ETS Translocation Variant 6 Protein
D015703:Antigens, CD; D014408:Biomarkers, Tumor; D050676:Core Binding Factor Alpha 2 Subunit; D008562:Membrane Glycoproteins; D015514:Oncogene Proteins, Fusion; D060245:Tetraspanin 29; C554869:CD9 protein, human; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein