Rat medium-term multi-organ carcinogenesis bioassay of Agaricus blazei Murrill fruit-body extract.
The modifying potential of Agaricus blazei Murrill fruit-body extract (ABFE) on tumor development was investigated in a medium-term multi-organ carcinogenesis bioassay. Male 6-week-old F344 rats were treated with N-nitrosodiethylamine (DEN), N-methyl-N-nitrosourea (MNU), 1,2-dimethylhydrazine dihydrochloride (DMH), N-butyl-N-(hydroxybutyl)-nitrosamine (BBN), and diisopropanolnitrosamine (DHPN) for initiation (DMBDD treatment). After a 1-week withdrawal period, the animals received distilled water (vehicle control) or ABFE A, gamma-amino butyric acid (GABA) at 0.8 mg/kg, ABFE B (GABA level of 3.0mg/kg) or ABFE C (GABA level of 12.0mg/kg) by gavage for 24 weeks. There were no effects of ABFE on survival rate, general condition, body weight, food and water consumption, and organ weights. The multiplicity of large intestinal nodules, smaller than 2mm was significantly increased in the ABFE C group with DMBDD treatment. However, there were no significantly inter-group differences in incidences of hyperplastic or neoplastic lesions in colon or other organs, or in immunohistochemically identified preneoplastic lesions in the liver. In conclusion, A. blazei Murrill fruit-body extract, even at a GABA level up to 12 mg/kg, did not exert modifying potential in the present medium-term multi-organ carcinogenesis bioassay in male F344 rats (DMBDD method).
Doi
Yuko
Y
DIMS Institute of Medical Science, Inc, 64 Goura, Nishiazai, Azai-cho, Ichinomiya 491-0113, Japan. doidoi@dims.co.jp
Furukawa
Fumio
F
Suguro
Mayuko
M
Ito
Hikaru
H
Imai
Norio
N
Nabae
Kyoko
K
Toda
Yosuke
Y
Inatomi
Satoshi
S
Kinugasa
Satomi
S
Kobayashi
Hitoshi
H
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D009369:Neoplasms / Q000139:chemically induced / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D016019:Survival Analysis; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002273:Carcinogens; D005680:gamma-Aminobutyric Acid; D005982:Glutathione Transferase