Purification and characterization of neoagarotetraose from hydrolyzed agar.
Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinase of melanoma cells was strongly inhibited by neoagarotetraose at a concentration of 1 micron/ml and similarly inhibited at 10 and 100 microg/ml compared to those by arbutin or kojic acid. The activity of mushroom tyrosinase showed a 38% inhibition by neoagarotetraose at 1 microg/ml, and this inhibitory effect was more efficient than that by kojic acid. Neoagarotetraose revealed similar IC(50) (50% inhibition concentration) value for mushroom tyrosinase as that by kojic acid. These data suggest that neoagarotetraose generated from agar by recombinant beta-agarase might be a good candidate as a cosmetic additive for whitening effect.
Jang
Ming-Kyung
MK
Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University, Busan 617-736, Korea.
Lee
Dong-Guen
DG
Kim
Nam-Young
NY
Yu
Ki-Hwan
KH
Jang
Hye-Ji
HJ
Lee
Sueng Woo
SW
Jang
Hyo Jung
HJ
Lee
Ye Ji
YJ
Lee
Sang-Hyeon
SH
D000362:Agar / Q000737:chemistry*; D000363:Agaricales / Q000201:enzymology; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D005697:Galactosides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D006868:Hydrolysis; D008543:Melanins / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011485:Protein Binding; D011753:Pyrones / Q000737:chemistry
D004791:Enzyme Inhibitors; D005697:Galactosides; D008543:Melanins; D009844:Oligosaccharides; D011753:Pyrones; C081798:neoagarotetraose; C011890:kojic acid; D000362:Agar; D014442:Monophenol Monooxygenase