Electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor.
Polypyrrole (Ppy) has been shown as a matrix for label-free electrochemical immunosensor based on electrochemical impedance spectroscopy (EIS) measurements. The immunosensing system model presented here was based on bovine leukemia virus (BLV) protein (gp51) entrapped within electrochemically-synthesized polypyrrole (Ppy/gp51). This Ppy/gp51 layer interacted with antibodies against gp51 (anti-gp51-Ab) that are present in significant concentration in the blood serum of BLV infected cattle. After this interaction protein complex (Ppy/gp51/anti-gp51-Ab) was formed. The horseradish peroxidase (HRP) labeled secondary antibodies (Ab) against anti-gp51-Ab were applied as agents interacting with Ppy/gp51/anti-gp51-Ab and forming the large protein complex (Ppy/gp51/anti-gp51-Ab/Ab). The EIS study was performed for electrodes modified with different Ppy layers described here and an optimal equivalent circuit was adopted for evaluation of EIS spectra, it was a major outcome of this study.
Ramanavicius
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Centre of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius 6, Lithuania. arunas@imi.lt
Finkelsteinas
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A
Cesiulis
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H
Ramanaviciene
A
A
D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D001909:Leukemia Virus, Bovine; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013057:Spectrum Analysis; D014764:Viral Proteins / Q000737:chemistry
D055767:Immobilized Proteins; D011108:Polymers; D011758:Pyrroles; D014764:Viral Proteins; C067635:polypyrrole