Non-natural nucleic acids for synthetic biology.
Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.
Appella
Daniel H
DH
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. appellad@niddk.nih.gov
D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D057005:Bioengineering / Q000379:methods*; D001695:Biology / Q000379:methods*; D005663:Furans / Q000737:chemistry; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry
D052157:Aptamers, Nucleotide; D005663:Furans; D009696:Nucleic Acids; D010710:Phosphates; C039281:furan