title: Cold-adapted yeasts from Antarctica and the Italian Alps-description of three novel species: Mrakia robertii sp. nov., Mrakia blollopis sp. nov. and Mrakiella niccombsii sp. nov.
abstract: Worldwide glaciers are annually retreating due to global overheating and this phenomenon determines the potential lost of microbial diversity represented by psychrophilic microbial population sharing these peculiar habitats. In this context, yeast strains, all unable to grow above 20 degrees C, consisting of 42 strains from Antarctic soil and 14 strains isolated from Alpine Glacier, were isolated and grouped together based on similar morphological and physiological characteristics. Sequences of the D1/D2 and ITS regions of the ribosomal DNA confirmed the previous analyses and demonstrated that the strains belong to unknown species. Three new species are proposed: Mrakia robertii sp. nov. (type strain CBS 8912), Mrakia blollopis sp. nov. (type strain CBS 8921) and a related anamorphic species Mrakiella niccombsii sp. nov. (type strain CBS 8917). Phylogenetic analysis of the ITS region revealed that the new proposed species were closely related to each other within the Mrakia clade in the order Cystofilobasidiales, class Tremellomycetes. The Mrakia clade now contains 8 sub-clades. Teliospores were observed in all strains except CBS 8918 and for the Mrakiella niccombsii strains.
authors.affiliation: School of Biological Sciences, University of New England, Armidale, NSW, Australia. skyethomashall@gmail.com
authors.forename: Skye Robin
authors.initials: SR
authors.lastname: Thomas-Hall
authors.forename: Benedetta
authors.initials: B
authors.lastname: Turchetti
authors.forename: Pietro
authors.initials: P
authors.lastname: Buzzini
authors.forename: Eva
authors.initials: E
authors.lastname: Branda
authors.forename: Teun
authors.initials: T
authors.lastname: Boekhout
authors.forename: Bart
authors.initials: B
authors.lastname: Theelen
authors.forename: Kenneth
authors.initials: K
authors.lastname: Watson
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D003080:Cold Temperature; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D007558:Italy; D010802:Phylogeny
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal