Two ply tubular scaffolds comprised of proteins/poliglecaprone/polycaprolactone fibers.
Electrospun bi-layer tubular hybrid scaffolds composed of poliglecaprone (PGC), polycaprolactone (PCL), elastin (E), and gelatin (G) were prepared and thereafter crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Scanning electron microscopic (SEM) images revealed a highly porous micro-structure comprising randomly distributed non-woven fibers with the majority of fibers in submicron diameters. The EDC-crosslinking yielded an average crosslinking degree of 40%. Uni-axial tensile test of hydrated scaffolds in both longitudinal and circumferential directions revealed tensile properties, comparable to those of native arteries. The graft (PGC:PCL = 1:3) did not demonstrate significant difference before and after EDC-crosslinking in tensile strength or % strain in either longitudinal or circumferential directions. However, crosslinking increased the Young's modulus of the graft along the longitudinal direction (from 5.84 to 8.67 MPa). On the contrary, the graft (3:1) demonstrated a significant decrease in maximum strain in both directions. Cyto-assay using human umbilical vein endothelial cells (HUVECs) showed excellent cell viability.
Zhang
Xing
X
Department of Biomedical Engineering, University of Alabama at Birmingham (UAB), Birmingham, AL, 35294, USA.
Thomas
Vinoy
V
Vohra
Yogesh K
YK
D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D002478:Cells, Cultured; D003460:Crystallization / Q000379:methods; D004146:Dioxanes / Q000737:chemistry*; D055119:Elastic Modulus; D004549:Elastin / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D004146:Dioxanes; D011091:Polyesters; C016240:polycaprolactone; C095495:glycolide E-caprolactone copolymer; D005780:Gelatin; D004549:Elastin