PCR detection of Histoplasma capsulatum var. capsulatum in whole blood of a renal transplant patient with disseminated histoplasmosis.
We report the identification of Histoplasma capsulatum var. capsulatum from whole blood in a renal transplant patient with disseminated histoplasmosis using colorimetric microtiter-plate PCR. This modality demonstrated utility in reaching a definitive diagnosis in a timely manner. Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis.
Qualtieri
Julianne
J
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Stratton
Charles W
CW
Head
David R
DR
Tang
Yi-Wei
YW
D000328:Adult; D005260:Female; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000097:blood* / Q000382:microbiology*; D006801:Humans; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling