title: Development and characterization of polymorphic microsatellite markers in Linum usitatissimum.
abstract: Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Deng
authors.forename: Songhua
authors.initials: S
authors.lastname: Long
authors.forename: Dongfeng
authors.initials: D
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Yufu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xinbo
authors.initials: X
authors.lastname: Chen
mesh_terms: D000483:Alleles; D001483:Base Sequence; D018900:Dinucleotide Repeats / Q000235:genetics; D019597:Flax / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*