Phoma herbarum as a new gibberellin-producing and plant growth-promoting fungus.
Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK- 2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially GA(4) and GA(7), were present in higher amounts (GA(1), 0.11 ng/ml; GA(3), 2.91 ng/ml; GA(4), 3.21 ng/ml; and GA(7), 1.4 ng/ml) in conjunction with physiologically inactive GA(9) (0.05 ng/ml), GA(12) (0.23 ng/ ml), GA(15) (0.42 ng/ml), GA(19) (0.53 ng/ml), and GA(20) (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence.
Hamayun
Muhammad
M
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea.
Khan
Sumera Afzal
SA
Khan
Abdul Latif
AL
Rehman
Gauhar
G
Sohn
Eun-Young
EY
Shah
Aamer Ali
AA
Kim
Sang-Kuk
SK
Joo
Gil-Jae
GJ
Lee
In-Jung
IJ
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018533:Biomass; D005875:Gibberellins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000254:growth & development* / Q000382:microbiology; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology*
D005875:Gibberellins