title: mRNA expression of matrix metalloproteinases (MMPs) 2 and 9 and tissue inhibitor of matrix metalloproteinases (TIMPs) 1 and 2 in childhood acute lymphoblastic leukemia: potential role of TIMP1 as an adverse prognostic factor.
abstract: This study evaluates the mRNA expression profile of genes TIMP1, TIMP2, MMP2 and MMP9 in diagnostic bone marrow samples from 134 consecutive ALL children by real-time quantitative PCR. A significant association was observed between higher expression levels of MMP9 and low risk group and absence of extramedullary infiltration and higher expression levels of TIMP2 and MMP2 with T-ALL. TIMP1 gene expression values higher than the median were associated with a significantly lower 5-year event free-survival in univariable (P=0.04) and multivariable analysis (P=0.01). Our data address new information in the complex interaction of the migration/adhesion genes and childhood ALL.
authors.affiliation: Department of Pediatrics, University of Sao Paulo, Ribeirao Preto, SP, Brazil. scrideli@fmrp.usp.br
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Scrideli
authors.forename: Maria Angélica Abdala
authors.initials: MA
authors.lastname: Cortez
authors.forename: José Andres
authors.initials: JA
authors.lastname: Yunes
authors.forename: Rosane Gomes de Paula
authors.initials: RG
authors.lastname: Queiróz
authors.forename: Elvis Terci
authors.initials: ET
authors.lastname: Valera
authors.forename: Juliana França
authors.initials: JF
authors.lastname: da Mata
authors.forename: Silvia Regina Caminada
authors.initials: SR
authors.lastname: Toledo
authors.forename: Priscila
authors.initials: P
authors.lastname: Pavoni-Ferreira
authors.forename: Maria Lúcia de Martino
authors.initials: ML
authors.lastname: Lee
authors.forename: Antonio Sérgio
authors.initials: AS
authors.lastname: Petrilli
authors.forename: Silvia Regina
authors.initials: SR
authors.lastname: Brandalise
authors.forename: Luiz Gonzaga
authors.initials: LG
authors.lastname: Tone
mesh_terms: D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9