title: [Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides].
abstract: OBJECTIVE
  To analyze and compare the cell growth and accumulation of flavonoids and chlorogenic acid in the callus and suspension cell of Eucommia ulmoides.
  
  
  METHODS
  The callus induced from the leaf of E. ulmoides seedlings were suspended in liquid medium. The time courses of cell growth and yields of flavonoids and chlorogenic acid were studied.
  
  
  RESULTS
  The highest contents of flavonoids and chlorogenic acid in the callus were 13.46, 1.712 mg x g(-1), respectively, while the contents of these two secondary metabolites were 16.63, 3.93 mg x g(-1) in suspension cell culture correspondingly.
  
  
  CONCLUSIONS
  Comparing with callus, the suspension cell showed a short growth period and high growth rate with a remarkable high content of flavonoids and chlorogenic acid.
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, College of Life Sciences Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Riming
authors.initials: R
authors.lastname: Yan
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Qiu
authors.forename: Qinggui
authors.initials: Q
authors.lastname: Zeng
authors.forename: Hai
authors.initials: H
authors.lastname: You
authors.forename: Du
authors.initials: D
authors.lastname: Zhu
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000032:analysis / Q000378:metabolism*; D031283:Eucommiaceae / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D002726:Chlorogenic Acid