title: Differential CXCR4 expression and function in subpopulations of the feline lymphoma cell line 3201 susceptible to feline immunodeficiency virus.
abstract: The infection of feline thymic lymphoma 3201 cells with a cell culture-adapted Petaluma strain of feline immunodeficiency virus (FIV) led to the establishment of survivor cells designated as 3201-S after a productive infection associated with extensive cell killing. 3201-S cells were free of FIV DNA, and were found to express CXCR4, a coreceptor for infection but not CD134, a primary receptor. When 3201-S cells were reinfected with FIV, viral DNA was transiently detectable for 5 days postinfection, indicating that 3201-S cells cannot support the FIV replicative cycle. Furthermore, comparative studies found that in contrast to SDF-1alpha-responsive 3201 cells, 3201-S cells did not show a flux of Ca(2+) in response to SDF-1alpha, implying that CXCR4 is not functionally active on 3201-S cells. These results suggest that 3201 cells can be heterogeneous in the phenotype of the CXCR4 expressed, and this heterogeneity may account for the differences in susceptibility to FIV. Determining the mechanism(s) within 3201-S cells that restrict FIV could result in therapeutic strategies against FIV infection.
authors.affiliation: Center for Integrative Education of Pharmacy Frontier, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. tad@pharm.kyoto-u.ac.jp
authors.forename: Tadafumi S
authors.initials: TS
authors.lastname: Tochikura
authors.forename: Kenji
authors.initials: K
authors.lastname: Motokawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Naito
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kozutsumi
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanabe-Tochikura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002415:Cats; D002461:Cell Line, Transformed; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D015752:Genes, env; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008214:Lymphocytes / Q000821:virology*; D019718:Receptors, CXCR4 / Q000378:metabolism* / Q000502:physiology; D015854:Up-Regulation
chemical_list: D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D004279:DNA, Viral; D019718:Receptors, CXCR4; D002118:Calcium