Leishmania cell surface prohibitin: role in host-parasite interaction.
Proteins selectively upregulated in infective parasitic forms could be critical for disease pathogenesis. A mammalian prohibitin orthologue is upregulated in infective metacyclic promastigotes of Leishmania donovani, a parasite that causes visceral leishmaniasis. Leishmania donovani prohibitin shares 41% similarity with mammalian prohibitin and 95-100% within the genus. Prohibitin is concentrated at the surface of the flagellar and the aflagellar pole, the aflagellar pole being a region through which host-parasite interactions occur. Prohibitin is attached to the membrane through a GPI anchor. Overexpression of wild-type prohibitin increases protein surface density resulting in parasites with higher infectivity. However, parasites overexpressing a mutant prohibitin with an amino acid substitution at the GPI anchor site to prevent surface expression through GPI-link show lesser surface expression and lower infective abilities. Furthermore, the presence of anti-prohibitin antibodies during macrophage-Leishmania interaction in vitro reduces infection. The cognate binding partner for Leishmania prohibitin on the host cell appears to be macrophage surface HSP70, siRNA mediated downregulation of which abrogates the capability of the macrophage to bind to parasites. Leishmania prohibitin is able to generate a strong humoral response in visceral leishmaniasis patients. The above observations suggest that prohibitin plays an important role in events leading to Leishmania-host interaction.
Jain
Rohit
R
Cell Death and Differentiation Research Laboratory, National Institute of Immunology, New Delhi-110067, India.
Ghoshal
Angana
A
Mandal
Chitra
C
Shaha
Chandrima
C
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D002460:Cell Line; D020868:Gene Silencing; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006790:Host-Parasite Interactions; D006801:Humans; D007893:Leishmania donovani / Q000472:pathogenicity*; D008264:Macrophages / Q000469:parasitology; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism; D000091402:Prohibitins; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015800:Protozoan Proteins / Q000276:immunology / Q000502:physiology*; D012097:Repressor Proteins / Q000276:immunology / Q000502:physiology*; D037521:Virulence Factors / Q000276:immunology / Q000502:physiology*
D000913:Antibodies, Protozoan; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D000091402:Prohibitins; D015800:Protozoan Proteins; D012097:Repressor Proteins; D037521:Virulence Factors