title: Improved membrane proteomics coverage of human embryonic stem cells by peptide IPG-IEF.
abstract: Protein biomarkers are fundamental tools for the characterization of stem cells and for tracking their differentiation and maturation down developmental lineages. Technology development allowing increased coverage of difficult cellular proteomes should allow for the discovery of new and novel membrane protein biomarkers for use by the stem cell research community. The amphipathic and highly hydrophobic nature and relative low abundance of many membrane proteins present significant analytical challenges. These difficulties are amplified when the source material (tissue or cells) is only available in limited quantities (e.g., embryonic stem cells). Recent advances in enrichment for purer membrane fractions, the enzymatic and chemical digestion of membrane proteins in the presence of solvents or chaotropes, and the use of "shotgun" proteomics methodologies have gradually resulted in increased membrane proteome coverage with numbers of predicted integral membrane proteins now in excess of 1000 being routinely reported. We have recently demonstrated the advantages of using peptide isoelectric focusing in the first dimension on immobilized pH gradients (peptide IPG-IEF) followed by reversed phase chromatography and tandem MS to increase membrane proteome coverage. This study looked at achieving a similar level of membrane proteome coverage using modifications to reported methodologies while restricting the number of characterized human embryonic stem cells to 10(7) cells. Two-thousand two-hundred and ninety-two (2292) nonredundant proteins were identified with two or more high accuracy peptide matches from 260 mug of a human embryonic stem cell membrane enriched fraction with a false discovery rate of 0.32%. Gene Ontology (GO) mapping predicted 1279 (44.9%) of this list to be membrane proteins of which 395 proteins were predicted to be derived from the plasma membrane compartment. The TMHMM algorithm predicted 904 integral membrane proteins with up to 16 transmembrane helices. Collectively, we assert that the substantial membrane proteome coverage achieved using these procedures will enable rapid advances in the identification and quantitation of novel membrane proteins as markers of differentiation status and/or genetic mutation from relatively low numbers of cultured embryonic stem cells.
authors.affiliation: Australian Proteome Analysis Facility, Faculty of Science, Macquarie University, NSW 2109, Australia.
authors.forename: Leon R
authors.initials: LR
authors.lastname: McQuade
authors.forename: Uli
authors.initials: U
authors.lastname: Schmidt
authors.forename: Dana
authors.initials: D
authors.lastname: Pascovici
authors.forename: Tomas
authors.initials: T
authors.lastname: Stojanov
authors.forename: Mark S
authors.initials: MS
authors.lastname: Baker
mesh_terms: D000465:Algorithms; D015415:Biomarkers; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000032:analysis*; D008722:Methods; D010455:Peptides / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D010455:Peptides