title: Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian species.
abstract: Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of nine Japanese amphibian species using reverse transcription polymerase chain reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and anuran amphibians formed two branches. Within the anuran species, three branches were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese amphibians).
authors.affiliation: Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku N18W9, Sapporo 060-0818, Japan.
authors.forename: Shouta M M
authors.initials: SM
authors.lastname: Nakayama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tanaka-Ueno
authors.forename: Kentaro Q
authors.initials: KQ
authors.lastname: Sakamoto
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fujita
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000663:Amphibians / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D007564:Japan
chemical_list: D003577:Cytochrome P-450 Enzyme System