title: Silent celiac disease in a cohort of healthy adults.
abstract: BACKGROUND
  Recent studies suggest that celiac disease (CD) is common in many developing countries. Because the disease may be under diagnosed in Cuba, we studied the presence of the disease in a group of apparently healthy adult.
  
  
  OBJECTIVE
  It was to assess for the first time, the presence of silent CD in a cohort of healthy Cuban adults individuals and to evaluate the tools for diagnosis of CD in this group.
  
  
  METHODS
  A total of 200 healthy Cuban adult from Havana City were evaluated. Tissue transglutaminase antibodies (tTGA) were determined by one-step immunochromatographic assay and by commercial ELISA kit. CD specific human leukocyte antigen (HLA) typing was performed by polymerase chain reaction amplification, using sequence-specific primers. In the subject positive for tTGA, the CD was confirmed by intestinal biopsy.
  
  
  RESULTS
  From the 200 studied individuals, only one subject was identified as positive by both assays, being submitted to duodenal biopsy. Morphological changes consistent with CD were found and also supported by HLA-DQ2 (HLA-DQA1*0501-DQB1*02). In the follow-up after one year, histological recovery was assessed by a second intestinal biopsy and the serological marker became negative.
  
  
  CONCLUSIONS
  This study confirms the existence of silent CD among healthy adult in Cuba and highlights the importance of mass screening for this disease among them. The one-step immunochromatographic assay is a good tool for this purpose.
authors.affiliation: Immunodiagnostics and Genomics Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. armando.galvan@cigb.edu.cu
authors.forename: José A
authors.initials: JA
authors.lastname: Galván
authors.forename: Gilda
authors.initials: G
authors.lastname: Lemos
authors.forename: María E
authors.initials: ME
authors.lastname: Fernández de Cossio
authors.forename: Caridad
authors.initials: C
authors.lastname: Ruenes
authors.forename: Yadina
authors.initials: Y
authors.lastname: Martínez
authors.forename: Yelaine
authors.initials: Y
authors.lastname: Tejeda
authors.forename: Juan
authors.initials: J
authors.lastname: Roca
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Palenzuela
authors.forename: Lidia I
authors.initials: LI
authors.lastname: Novoa
authors.forename: Marcelo
authors.initials: M
authors.lastname: Nazabal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood / Q000276:immunology; D002446:Celiac Disease / Q000175:diagnosis* / Q000178:diet therapy / Q000453:epidemiology* / Q000276:immunology; D015331:Cohort Studies; D003462:Cuba / Q000453:epidemiology; D055050:Diet, Gluten-Free; D004386:Duodenum / Q000473:pathology; D005260:Female; D019204:GTP-Binding Proteins / Q000276:immunology; D006683:HLA-DQ Antigens / Q000276:immunology; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006262:Health; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000276:immunology; D055815:Young Adult
chemical_list: D000906:Antibodies; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D007070:Immunoglobulin A; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins