[Isolation and determination of anamorph of Shiraia bambusicola].
OBJECTIVE
To isolate and identify the anamorph of Shiraia bambusicola.


METHODS
Fungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.


RESULTS
The strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.


CONCLUSIONS
The isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.
Wen
Du
D
Institute of Fungus Resources, Guizhou University, Guiyang 550025, China. duwen6688@163.com
Han
Yanfeng
Y
Liang
Jiandong
J
Liang
Zongqi
Z
D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S