Ion-exchange chromatography.
Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.
Jungbauer
Alois
A
Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Hahn
Rainer
R
D000818:Animals; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D006801:Humans; D019995:Laboratory Chemicals / Q000737:chemistry; D011485:Protein Binding / Q000502:physiology
D019995:Laboratory Chemicals