DNA damage detected by the alkaline comet assay in the liver of mice after oral administration of tetrachloroethylene.
Induction of DNA damage in the liver and kidney of male CD1 mice was studied by means of the alkaline Comet assay after oral administration of tetrachloroethylene at the doses of 1000 and 2000 mg/kg/day. A statistically significant dose-related increase in tail intensity was established in hepatocytes, indicating that tetrachloroethylene induced DNA damage in the liver. No effect on DNA damage was observed in the kidney. The results are in agreement with carcinogenicity data in mice, in which tetrachloroethylene induced tumours in the liver but not in the kidney, and support that a genotoxic mode of action might be involved in liver carcinogenicity in mice. An alternative interpretation of the results conveyed by the Study director at the test facility, involving that tetrachloroethylene did not induce DNA damage in the liver and kidney of mice, is also presented and discussed.
Cederberg
Håkan
H
Swedish Chemicals Agency, PO Box 2, S-172 13 Sundbyberg, Sweden. Hakan.Cederberg@kemi.se
Henriksson
Jörgen
J
Binderup
Mona-Lise
ML
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D013750:Tetrachloroethylene / Q000633:toxicity*
D002273:Carcinogens; D013750:Tetrachloroethylene