title: Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter.
abstract: Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enhancement of antagonistic activities against Bacillus cereus. HPLC peaks of surfactin for recombinant fmbR-1 showed patterns of lipopeptides similar to those of the wild-type strain, and surfactin production of the recombinant fmbR-1 was up to about fivefold greater than that of the wild-type strain without induction by isopropyl beta--1-thiogalactopyranoside. However, the production of surfactin increased to about 10-fold more than that of the wild-type strain when it was induced by isopropyl beta--1-thiogalactopyranoside.
authors.affiliation: Nanjing Agricultural University, Weigang, Nanjing Jiangsu, China.
authors.forename: Huigang
authors.initials: H
authors.lastname: Sun
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Lu
authors.forename: Yundailai
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D055666:Lipopeptides / Q000096:biosynthesis*; D009876:Operon; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide