Aromatic hydroxylation of indan by o-xylene-degrading Rhodococcus sp. strain DK17.
The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.
Kim
Dockyu
D
Polar BioCenter, Korea Polar Research Institute, KORDI, Incheon, South Korea.
Lee
Choong Hwan
CH
Choi
Jung Nam
JN
Choi
Ki Young
KY
Zylstra
Gerben J
GJ
Kim
Eungbin
E
D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D006900:Hydroxylation; D007189:Indans / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D010088:Oxidoreductases / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012240:Rhodococcus / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
D001426:Bacterial Proteins; D007189:Indans; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014992:Xylenes; D010088:Oxidoreductases; C093582:indan; C026114:2-xylene