title: Simple and rapid screening for HLA-Cw*06 in Polish patients with psoriasis.
abstract: BACKGROUND
  The human leucocyte antigen (HLA) C allele Cw*06 is currently recognized as a major disease allele at the PSORS1 locus. It has been suggested that characterization of this gene could be used as a convenient criterion for classification of psoriasis phenotypes.
  
  
  OBJECTIVE
  To design and optimize a DNA typing procedure, suitable for identification of HLA-Cw*06 and its zygosity status verification in large-scale analyses, and to test for its robustness in a case-control study.
  
  
  METHODS
  PCR assays with sequence-specific primers (PCR-SSP) were used for specific detection of HLA-Cw*06. PCR with analysis of restriction fragment length polymorphism was used to distinguish between patients homozygous and heterozygous for HLA-Cw*06. Additionally, those homozygous for HLA-Cw*06 were screened for nonspecific digestion by degenerated PCR-SSP. This three-step procedure was used in the examination of 383 patients with psoriasis that developed at the age of >or= 30 years of age and of 143 healthy subjects from northern Poland.
  
  
  RESULTS
  A simple and rapid procedure for screening of HLA-Cw*06 was produced. A significant difference in HLA-Cw*06 frequency between patients with psoriasis and controls was seen (P = 0.02). Detailed examination of the age of disease onset among patients with psoriasis revealed that involvement of HLA-Cw*06 in the genetic background of psoriasis developing as late as the age of 45 years cannot be neglected.
  
  
  CONCLUSIONS
  The low cost, high-throughput capacity and requirement for small sample amounts make this procedure a useful one for HLA-Cw*06 typing in clinical practice and large population studies. We recommend that patients with psoriasis diagnosed before 45 years of age should be considered for diagnostic HLA-Cw*06 typing.
authors.affiliation: Department of Forensic Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Rebała
authors.forename: A
authors.initials: A
authors.lastname: Szczerkowska-Dobosz
authors.forename: K
authors.initials: K
authors.lastname: Niespodziana
authors.forename: J
authors.initials: J
authors.lastname: Wysocka
mesh_terms: D000328:Adult; D017668:Age of Onset; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011565:Psoriasis / Q000235:genetics*
chemical_list: D015236:HLA-C Antigens; C528994:HLA-C*06 antigen