Formation of size-controlled nano carrier systems by self-assembly.
Nano carrier systems were prepared by forming self-assembled liposomes having a size distribution in the nano range through use of an ultrasonic homogenizer. Phosphatidylcholine and cholesterol were utilized as an amphiphilic compound and a shape stabilizer, respectively. The size of prepared samples was decreased (up to 150 nm) by elevating ratio of lecithin and extending homogenization time (2 to approximately 6 min). After secondary coating with alginic acid (0.1, 0.3 and 0.5%, W/V), size was remarkably changed in the range of +/-30 nm and zeta-potential was altered (only chitosan coating (molecular weight: 30 000 Da, 0.2%, W/V): 10.3 mV, Alginic acid coating (0.5%, W/V) after the chitosan coating: -21.8 mV). The low molecular weight chitosan (0.1%, W/V)-coated nano-liposomes had a lower absolute value of zeta-potential than the high molecular weight chitosan (0.1%, W/V)-coated nano-liposomes. The encapsulation efficiency was measured by gas chromatography. The efficiency was decreased slightly by elevating chitosan concentration (0.1 to approximately 0.5%, W/V).
Bang
S H
SH
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, South Korea.
Yu
Y M
YM
Hwang
I C
IC
Park
H J
HJ
D000464:Alginates; D002214:Capsules / Q000138:chemical synthesis* / Q000737:chemistry; D048271:Chitosan; D002784:Cholesterol; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010713:Phosphatidylcholines; D014465:Ultrasonics
D000464:Alginates; D002214:Capsules; D004337:Drug Carriers; D006603:Hexuronic Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D020723:Glucuronic Acid; D048271:Chitosan; D002784:Cholesterol