Ammonium sulfate and MALDI in-source decay: a winning combination for sequencing peptides.
In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay (ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.
Delvolve
Alice
A
Cellular Neurobiology, NIDA IRP, NIH, Baltimore, Maryland 21224, USA.
Woods
Amina S
AS
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000737:chemistry*; D053000:Analytic Sample Preparation Methods; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein / Q000191:economics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides; D000645:Ammonium Sulfate