Adeno-associated virus type 5 utilizes alternative translation initiation to encode a small Rep40-like protein.
Alternative splicing of adeno-associated virus type 2 (AAV2) P19-generated pre-mRNAs generates the small Rep proteins Rep52 and Rep40, which differ in their carboxyl termini. Both proteins are required for optimal packaging of AAV2 genomes. AAV5 Rep-encoding P19-generated transcripts are primarily polyadenylated within the central intron and not efficiently spliced; however, surprisingly, AAV5 was found to generate high levels of a Rep40-like protein. The AAV5 Rep40-like protein was generated by internal initiation and has the same C terminus as Rep52. Although precluded from using alternative splicing to generate multiple Rep isoforms, AAV5 ensures the production of a Rep40-like protein by utilizing a novel internal translation initiation event.
Farris
K David
KD
Department of Molecular Microbiology, University of Missouri-Columbia, School of Medicine, Bond Life Sciences Center, Columbia, Missouri 65211, USA.
Pintel
David J
DJ
D017398:Alternative Splicing; D000818:Animals; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000229:Dependovirus / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D015967:Gene Expression Regulation, Viral; D006801:Humans; D009154:Mutation; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism
D012367:RNA, Viral; D004265:DNA Helicases; C477740:Rep40 protein, adeno-associated virus