title: Piezo-adapted 3-isopropylmalate dehydrogenase of the obligate piezophile Shewanella benthica DB21MT-2 isolated from the 11,000-m depth of the Mariana Trench.
abstract: 3-isopropylmalate dehydrogenase (IPMDH)-encoding leuB genes were obtained from the obligate piezophile Shewanella benthica DB21MT-2 and non-piezophile Shewanella oneidensis MR-1. The genes were expressed in Escherichia coli and the proteins were purified using His-tag. The estimated kinetic parameters of these enzymes indicated that IPMDH of S. benthica DB21MT-2 is more tolerant of high pressure than that of S. oneidensis MR-1. Thus such an adaptation is one of the mechanisms bacteria utilize for survival at high pressures.
authors.affiliation: Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kasahara
authors.forename: Takako
authors.initials: T
authors.lastname: Sato
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamegai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000222:Adaptation, Physiological; D055162:Biocatalysis; D002846:Chromatography, Affinity; D004586:Electrophoresis; D006639:Histidine / Q000378:metabolism; D006874:Hydrostatic Pressure; D010140:Pacific Ocean; D020592:Shewanella / Q000201:enzymology* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D006639:Histidine; D050539:3-Isopropylmalate Dehydrogenase