title: Drying stress and damage processes in human stratum corneum.
abstract: The drying stresses that develop in stratum corneum (SC) are crucial for its mechanical and biophysical function, its cosmetic feel and appearance, and play a central role in processes of dry skin damage. However, quantitative methods to characterize these stresses are lacking and little understanding exists regarding the effects of drying environment, chemical exposures and moisturizing treatments. We describe the application of a substrate curvature technique adapted for biological tissue to accurately characterize SC drying stresses as a function of time following environmental pre-conditioning and chemical treatment in a range of drying environments. SC stresses were observed to increase to stress levels of up to approximately 3 MPa over periods of 8 h depending on pretreatment and drying environment. A unique relationship between the SC stress and water in the drying environment was established. The effect of glycerol on lowering SC stresses and damaging surfactants on elevating SC stresses were quantified. Extensions of the method to continuous monitoring of SC stresses in response to changes in environmental moisture content and temperature are reported. Finally, a biomechanics framework to account for the SC drying stress as a mechanical driving force for dry skin damage is presented.
authors.affiliation: Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
authors.forename: K
authors.initials: K
authors.lastname: Levi
authors.forename: R J
authors.initials: RJ
authors.lastname: Weber
authors.forename: J Q
authors.initials: JQ
authors.lastname: Do
authors.forename: R H
authors.initials: RH
authors.lastname: Dauskardt
mesh_terms: D000368:Aged; D003358:Cosmetics / Q000494:pharmacology*; D005260:Female; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D012867:Skin / Q000737:chemistry / Q000503:physiopathology*; D013718:Tensile Strength / Q000502:physiology; D014870:Water Loss, Insensible / Q000187:drug effects / Q000502:physiology*
chemical_list: D003358:Cosmetics; D005990:Glycerol