Studies on the Bohr Effect of Sheep Hemoglobin.
A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate [PCMB] treated hemoglobin. The influence of a number of sulfhydryl inhibitors on the Bohr effect of a hemoglobin solution is reported and an interpretation based on steric factors in the protein is proposed.
Smyth
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Departments of Biochemistry and Physiology, Yale University School of Medicine, New Haven.
Battaglia
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Meschia
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