Cross-reaction between proteins of Larrea divaricata Cav. (jarilla) and proteins of Gram-negative bacteria.
Larrea divaricata is an abundant plant of northwest of Argentina used to treat different pathologies. We aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and whole cell-bacterial proteins (W-CBP) of Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Klebsiella pneumoniae using a mouse anti-JPCE serum. Protein profiles of JPCE and W-CBP were analyzed. For JPCE, 18 bands were observed in a 20-176 kDa range. Levels of IgG against JPCE and W-CBP were determined. Bacterial proteins showed a strong reaction with the anti-JPCE serum. Plant proteins could be used as immune stimulants.
de Anaya
María Aída Mattar
MA
Microbiology Unit, Department of Biochemistry and Biological Sciences, Faculty of Chemistry and Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
Davicino
Roberto
R
Casali
Yolanda
Y
Correa
Silvia
S
Micalizzi
Blas
B
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000276:immunology; D032442:Larrea / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000276:immunology / Q000378:metabolism; D018515:Plant Leaves / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011511:Proteus / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology
D001426:Bacterial Proteins; D000939:Epitopes; D010936:Plant Extracts; D010940:Plant Proteins