Changes in plasma testosterone level and semen quality after frequent injections of GnRH analogue in a Beagle dog with azoospermia.
A Beagle with a low plasma testosterone (T) level and azoospermia was given 10 subcutaneous injections of 1 microg gonadotropin-releasing hormone analogue (GnRH-A) per head at intervals of 3 days (Experiment 1), and 6 months after the final injection was given, 15 subcutaneous injections of 2 microg GnRH-A were given at intervals of 2 days (Experiment 2). The plasma T level increased and peaked at 8 weeks after the first injection of GnRH-A in both Experiment 1 and Experiment 2. Motile sperm were detected in the semen collected 8 weeks and 7 weeks after the first injection in Experiment 1 and Experiment 2, respectively. The total number of sperm peaked 9 weeks after the first injection in both Experiment 1 (4.5 x 10(6)) and Experiment 2 (72.8 x 10(6)).
Kawakami
Eiichi
E
Laboratory of Veterinary Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan. Kawakami@nvlu.ac.jp
Hori
Tatsuya
T
Tsutsui
Toshihiko
T
D000818:Animals; D053713:Azoospermia / Q000188:drug therapy / Q000662:veterinary*; D004285:Dogs; D004334:Drug Administration Schedule; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage* / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000187:drug effects*; D013739:Testosterone / Q000097:blood*
D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone