[Proliferation of the mesenchymal stem cells in a delayed fat flap: an experimental study in rabbits].
OBJECTIVE
To investigate the possibility to enhance the proliferation of adipose-derived stem cells (ASCs) in a delayed fat flap in rabbits.


METHODS
A delayed fat flap was formed in one side of inguinal region of a rabbit. 21 days after operation, the fat tissues at the delayed flaps and at the unoperated side were harvested and digested with 0.25% collagenase and sieved. The cell suspensions were centrifuged. The cells were obtained from tissue precipitate after centrifugation. The expression rates of the surface marker (CD29, CD44, CD14 and CD45) were measured by FCM and compared between the experimental and control groups.


RESULTS
Expression rates of CD29 and CD44 were higher in the delayed fat flap (74.06% and 90.74%) than in the contralateral fat tissue (62.88% and 77.54%, P < 0.05), while those of CD14 and CD45 were lower in the delayed fat flap (57.66% and 4.84%) than in the contralateral fat tissue (72.10% and 75.82%, P < 0.05 and P < 0.01).


CONCLUSIONS
Tissue hypoxic ischemia such as fat tissue in a delayed fat flap can promote proliferation of ASCs. It indicates that tissue in the delayed flap may be transplanted with better survival rate. The ischemia pretreatment of fat tissue may become a new method for fat transplantation.
Jiang
Su-Jun
SJ
Clinical Medical College of Hangzhou Normal University, Hangzhou 310036, China.
He
Xiao-Sheng
XS
Ni
You-Di
YD
Liu
Mao-Lin
ML
Liu
Su-Hang
SH
Zhong
Xiao-Chun
XC
D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D006085:Graft Survival; D011184:Postoperative Period; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology*; D013524:Surgical Flaps