mRNA expression of matrix metalloproteinases (MMPs) 2 and 9 and tissue inhibitor of matrix metalloproteinases (TIMPs) 1 and 2 in childhood acute lymphoblastic leukemia: potential role of TIMP1 as an adverse prognostic factor.
This study evaluates the mRNA expression profile of genes TIMP1, TIMP2, MMP2 and MMP9 in diagnostic bone marrow samples from 134 consecutive ALL children by real-time quantitative PCR. A significant association was observed between higher expression levels of MMP9 and low risk group and absence of extramedullary infiltration and higher expression levels of TIMP2 and MMP2 with T-ALL. TIMP1 gene expression values higher than the median were associated with a significantly lower 5-year event free-survival in univariable (P=0.04) and multivariable analysis (P=0.01). Our data address new information in the complex interaction of the migration/adhesion genes and childhood ALL.
Scrideli
Carlos Alberto
CA
Department of Pediatrics, University of Sao Paulo, Ribeirao Preto, SP, Brazil. scrideli@fmrp.usp.br
Cortez
Maria Angélica Abdala
MA
Yunes
José Andres
JA
Queiróz
Rosane Gomes de Paula
RG
Valera
Elvis Terci
ET
da Mata
Juliana França
JF
Toledo
Silvia Regina Caminada
SR
Pavoni-Ferreira
Priscila
P
Lee
Maria Lúcia de Martino
ML
Petrilli
Antonio Sérgio
AS
Brandalise
Silvia Regina
SR
Tone
Luiz Gonzaga
LG
D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9