title: Protective effect of stress-induced liver damage by saponin fraction from Codonopsis lanceolata.
abstract: Saponins are valuable principles found in various herbal medicine with pharmaceutical, cosmetical and nutraceutical merits. In this study, we evaluated the protective role of saponin fraction (Cl-SF), prepared from Codonopsis lanceolata, an ethnopharmacologically famous plant in Korea, China and Japan, on water immersion stress-induced liver damage and radical generation. Cl-SF clearly decreased the up-regulated levels of serum glutamate-oxalacetate transaminase and glutamate-pyruvate-transaminase induced by water-immersed stress conditions. Furthermore, Cl-SF seemed to block the stress-induced radicals. Thus, Griess and DPPH assays revealed that Cl-SF significantly suppressed both radical generation in sodium nitroprusside-treated RAW264.7 cells and nitric oxide production in LPS-treated RAW264.7 cells. Therefore, these results suggest that Cl-SF may be considered as a promising stress-regulatory principle with radical scavenging actions.
authors.affiliation: School of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Kim
authors.forename: Jaehwi
authors.initials: J
authors.lastname: Lee
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoo
authors.forename: Yong Gyu
authors.initials: YG
authors.lastname: Lee
authors.forename: Se Eun
authors.initials: SE
authors.lastname: Byeon
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D029745:Codonopsis / Q000737:chemistry*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007101:Immersion; D008107:Liver Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010851:Picrates / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D013315:Stress, Psychological / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D017382:Reactive Oxygen Species; D012503:Saponins; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl