Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells.
Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC(50) value of cimiside E on gastric cancer cells for 24 h was 14.58 microM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 microM) and G2/M phase at higher concentrations (60 and 90 microM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
Guo
Lian Yu
LY
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.
Joo
Eun Ji
EJ
Son
Kun Ho
KH
Jeon
Su Jin
SJ
Jang
Sehyun
S
Shin
Eun Myoung
EM
Zhou
Hong Yu
HY
Kim
Yeong Shik
YS
D017304:Annexin A5 / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031885:Cimicifuga / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D019014:fas Receptor / Q000378:metabolism
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D012503:Saponins; D014315:Triterpenes; C545903:cimiside E; D019014:fas Receptor