Expression of matrix metalloproteinase-1, -7, -9, -13, Ki-67, and HER-2 in epithelial-myoepithelial salivary gland cancer.
BACKGROUND
The expression of matrix metalloproteinases (MMPs) in epithelial-myoepithelial salivary gland carcinoma has not been studied previously.


METHODS
Immunohistochemistry for MMP-1, -7, -9, -13, Ki-67, and HER-2, as well as HER-2 gene amplification by silver enhanced in situ hybridization was performed in a series of 12 paraffin-embedded histopathologic samples of patients from Canada and Finland.


RESULTS
A positive MMP-13 (p = .0022), higher MMP-13 (p = .0274), and higher MMP-9 (p = .0274) index (multiplication of staining intensity by percentage of the positive cells) predicted better overall survival. In disease-specific analysis, higher MMP-9 index (p = .0327) predicted better survival. A higher volume corrected index (VCI) of Ki-67 (p = .0339) predicted worse disease-specific survival. In 1 patient, HER-2 oncogene amplification was observed.


CONCLUSIONS
MMPs and Ki-67 may have prognostic impact in epithelial-myoepithelial carcinoma.
Luukkaa
Heikki
H
Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland.
Klemi
Pekka
P
Leivo
Ilmo
I
Mäkitie
Antti A
AA
Irish
Jonathan
J
Gilbert
Ralph
R
Perez-Ordonez
Bayardo
B
Hirsimäki
Pirkko
P
Vahlberg
Tero
T
Kivisaari
Atte
A
Kähäri
Veli-Matti
VM
Grénman
Reidar
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism*; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*
D019394:Ki-67 Antigen; D053505:Matrix Metalloproteinases, Secreted; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human