Morphology of testes from transgenic rabbits: histological and ultrastructural aspects.
The aim of this study was to compare morphological characteristics of testes from transgenic (the WAP-hFVIII gene) and non-transgenic rabbits with emphasis on the histological and ultrastructural aspects. Samples of testes from both groups were fixed and embedded into Durcupan ACM for transmission electron microscopy. For histological analysis, semi-thin toluidine blue-stained sections were evaluated under a Jenaval light microscope. Male fertility was tested based on egg fecundity and blastocyst yield; transgene transmission was proved using PCR assay. Spermatogenesis in rabbit testes had not been destroyed both in transgenic and non-transgenic rabbits. No significant differences were found in the occurrence of individual cell organelles of the Sertoli cells in transgenic and non-transgenic rabbits. The ultrastructure of Leydig cells in testes of transgenic and non-transgenic rabbits was rather similar. No differences in the occurrence of individual organelles of Leydig cells between transgenic and non-transgenic males were found. These results were in concert with fertilizing capacity of transgenic spermatozoa. The presented status of organelles in this study indicates functional activity of the analysed cells.
Chrenek
P
P
Animal Production Research Centre, Nitra, LuZianky, Slovakia.
Makarevich
A V
AV
Kubovicová
E
E
Pivko
J
J
D000818:Animals; D030801:Animals, Genetically Modified; D005298:Fertility; D005306:Fertilization; D007985:Leydig Cells / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D011817:Rabbits; D012708:Sertoli Cells / Q000166:cytology / Q000648:ultrastructure; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure; D013737:Testis / Q000033:anatomy & histology / Q000166:cytology* / Q000648:ultrastructure