title: Aromatic hydroxylation of indan by o-xylene-degrading Rhodococcus sp. strain DK17.
abstract: The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.
authors.affiliation: Polar BioCenter, Korea Polar Research Institute, KORDI, Incheon, South Korea.
authors.forename: Dockyu
authors.initials: D
authors.lastname: Kim
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Jung Nam
authors.initials: JN
authors.lastname: Choi
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Choi
authors.forename: Gerben J
authors.initials: GJ
authors.lastname: Zylstra
authors.forename: Eungbin
authors.initials: E
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D006900:Hydroxylation; D007189:Indans / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D010088:Oxidoreductases / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012240:Rhodococcus / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007189:Indans; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014992:Xylenes; D010088:Oxidoreductases; C093582:indan; C026114:2-xylene