title: [Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain].
abstract: OBJECTIVE
  To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine.
  
  
  METHODS
  By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter.
  
  
  RESULTS
  Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer.
  
  
  CONCLUSIONS
  The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.
authors.affiliation: Graduate School, Jilin Agriculture University, Changchun 130118, China. piaogl110@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Jinying
authors.initials: J
authors.lastname: Ge
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D005821:Genetic Techniques; D011818:Rabies / Q000276:immunology / Q000821:virology*; D011819:Rabies Vaccines / Q000235:genetics* / Q000276:immunology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D011819:Rabies Vaccines; D014764:Viral Proteins