The effect of regular training on plasma cytokines response in healthy and diabetic rats.
OBJECTIVE
To evaluate the effect of regular swimming exercise on plasma levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and of IL-6 cytokines in healthy and diabetic rats.


METHODS
In this study, carried out from March 2008 to March 2009 in the Drug Applied Research Center, Tabriz, Iran, 40 Wistar rats (250-300 g) were randomly divided into 4 groups (n=10): control-sedentary, control-exercised, diabetic-sedentary, and diabetic-exercised. Diabetes was induced by a single injection of streptozotocin (50 mg/kg, intraperitoneally). The exercise protocol consisted of swimming (one hour/day, and 5 days/week) for 8 weeks. The plasma cytokines were measured by using specific kits and the enzyme-linked immunosorbent assay method.


RESULTS
The findings of this study showed that the mean difference of IL-6 was significant among the groups, and that regular swimming increased the plasma levels of IL-6 to 9-times in healthy rats and to 23-times in diabetic ones (p=0.000, F (3,31)=54.79). However, there were no significant differences in IL-1beta, and TNF-alpha levels among the groups.


CONCLUSIONS
According to findings of this study, regular exercise causes an increase in plasma levels of IL-6, and this enhancement is much higher in diabetics rather than healthy rats. Thus, by increasing direct absorption of blood glucose by skeletal muscle, IL-6 can have a beneficial role in continuing the activities of diabetic patients.
Bonyadi
Mohammadreza
M
Department of Immunology, Faculty of Medicine, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. bonyadir@tbzmed.ac.ir
Badalzadeh
Reza
R
Mohammadi
Mustafa
M
Poozesh
Shahindokht
S
Salehi
Iraj
I
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D016207:Cytokines / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018836:Inflammation Mediators / Q000097:blood*; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D010805:Physical Conditioning, Animal; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013311:Streptozocin; D013550:Swimming; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D001786:Blood Glucose; D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin