title: [MALDI-TOF mass spectrometry in the investigation of large high-molecular biological compounds].
abstract: MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry has become, in the recent years, a tool of choice for analyses of biological polymers. The wide mass range, high accuracy, informativity and sensitivity make it a superior method for analysis of all kinds of high-molecular biological compounds including proteins, nucleic acids and lipids. MALDI-TOF-MS is particularly suitable for the identification of proteins by mass fingerprint or microsequencing. Therefore it has become an important technique of proteomics. Furthermore, the method allows making a detailed analysis of post-translational protein modifications, protein-protein and protein-nucleic acid interactions. Recently, the method was also successfully applied to nucleic acid sequencing as well as screening for mutations.
authors.forename: L V
authors.initials: LV
authors.lastname: Porubl'ova
authors.forename: A V
authors.initials: AV
authors.lastname: Rebriiev
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Hromovyĭ
authors.forename: I I
authors.initials: II
authors.lastname: Minia
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Obolens'ka
mesh_terms: D008055:Lipids / Q000737:chemistry*; D008970:Molecular Weight; D009696:Nucleic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins