Antifungal effect with apoptotic mechanism(s) of Styraxjaponoside C.
The antifungal effects and mechanisms of Styraxjaponoside C were investigated. Styraxjaponoside C was active against several human pathogens, including Candida albicans. Styraxjaponoside C induced a series of cellular changes characteristic of apoptosis in C. albicans, including increased reactive oxygen species (ROS) production, measured by DHR-123 staining; phosphatidylserine externalization, visualized by Annexin V staining; DNA fragmentation, as seen by TUNEL; and plasma membrane depolarization, observed by DiBAC(4)(3) staining. The plasma membrane depolarization is likely to be associated with production of ROS. The current study suggests that Styraxjaponoside C exerts an antifungal effect by promoting apoptosis.
Park
Cana
C
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea.
Woo
Eun-Rhan
ER
Lee
Dong Gun
DG
D000935:Antifungal Agents / Q000494:pharmacology*; D017209:Apoptosis; D002176:Candida albicans / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D008290:Malassezia / Q000187:drug effects; D014250:Trichosporon / Q000187:drug effects
D000935:Antifungal Agents; D005960:Glucosides; D017705:Lignans; C546201:fargesin-4-O-glucopyranoside