Progress in single-molecule spectroscopy in cells.
Time-dependent single-molecule spectroscopy allows the direct observation of molecular processes in real time, capturing transient and stochastic events that cannot be detected using conventional ensemble-averaged methods. It offers a promising approach to uncovering the physical and chemical transformations underlying cellular responses. Recent advances in the probe development, specific labeling strategies, and spectroscopy instrumentation suggest the feasibility of monitoring a single molecular machine's actions inside a living cell.
Yang
Haw
H
Department of Chemistry, Princeton University, Princeton, NJ 08544, USA. hawyang@princeton.edu
D002477:Cells / Q000166:cytology / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry; D015336:Molecular Probe Techniques; D049329:Nanostructures / Q000737:chemistry; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D046911:Macromolecular Substances