Cloning and bioinformatic identification of small RNAs in the filarial nematode, Brugia malayi.
Characterization of small RNAs from the filarial nematode Brugia malayi is the initial step in understanding their role in gene silencing. Both RNA cloning and bioinformatics were used to identify 32 microRNAs (miRNAs) belonging to 24 families. One family, miR-36 only occurs in helminths including B. malayi. Several of the miRNAs are arranged in clusters and are coordinately expressed as determined by northern blot analysis. In addition, small RNAs were identified from Pao/Bleo retrotransposons and their associated repeat sequences indicating that B. malayi uses an RNAi mechanism to maintain genome integrity. Analysis of these data provides a first glimpse into how small RNA-mediated silencing pathways regulate the parasitic life cycle of B. malayi.
Poole
Catherine B
CB
New England Biolabs, 240 County Rd, Ipswich, MA 01938, USA. poole@neb.com
Davis
Paul J
PJ
Jin
Jingmin
J
McReynolds
Larry A
LA
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D017178:Brugia malayi / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D018510:RNA, Helminth / Q000235:genetics*
D035683:MicroRNAs; D018510:RNA, Helminth