Inhibition of prostate cancer by suicide gene targeting the FCY1 and HSV-TK genes.
Prostate cancer is one of the most prevalent tumors. The switch of androgen signal dependence makes therapy more complex. Although reports on introduction of a single suicide gene exist, double suicide gene therapy has not been reported yet. In the current study, two suicide genes were constructed in the pIRES plasmid driven by PSMA promoter. 5-FC and GCV combination in vitro led to a higher growth inhibition on prostate cancer compared to a single pro-drug. Retarded xenograft tumor growth was observed in castrated nude mice after double suicide gene activation. Furthermore, decreased metastasis was observed with double suicide gene treatment. These findings suggest that specific double suicide gene strategy could be a potential option for the therapy of prostate cancer.
Yue
Qiao-Hong
QH
Department of Clinic Molecular Research Center and Clinic Diagnostic Laboratory, Xijing Hospital, the Fourth Military Medical University, Xi'an, PR China.
Hu
Xing-Bin
XB
Yin
Ying
Y
Su
Ming-Quan
MQ
Cheng
Xiao-Dong
XD
Yang
Liu
L
Zhou
Tie-Cheng
TC
Hao
Xiaoke
X
D045744:Cell Line, Tumor; D005260:Female; D041722:Genes, Transgenic, Suicide / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008957:Models, Genetic; D009362:Neoplasm Metastasis; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D018139:Simplexvirus / Q000235:genetics*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013937:Thymidine Kinase / Q000235:genetics*; D016896:Treatment Outcome
D013778:Tetrazolium Salts; D013844:Thiazoles; D013937:Thymidine Kinase; C022616:thiazolyl blue