title: Experimental investigation of evanescence-based infrared biodetection technique for micro-total-analysis systems.
abstract: The advent of microoptoelectromechanical systems (MOEMS) and its integration with other technologies such as microfluidics, microthermal, immunoproteomics, etc. has led to the concept of an integrated micro-total-analysis systems (microTAS) or Lab-on-a-Chip for chemical and biological applications. Recently, research and development of microTAS have attained a significant growth rate over several biodetection sciences, in situ medical diagnoses, and point-of-care testing applications. However, it is essential to develop suitable biophysical label-free detection methods for the success, reliability, and ease of use of the microTAS. We proposed an infrared (IR)-based evanescence wave detection system on the silicon-on-insulator platform for biodetection with microTAS. The system operates on the principle of bio-optical interaction that occurs due to the evanescence of light from the waveguide device. The feasibility of biodetection has been experimentally investigated by the detection of horse radish peroxidase upon its reaction with hydrogen peroxide.
authors.affiliation: Concordia University, Department of Mechanical Engineering, Optical-Bio Microsystems Laboratory, 1515 Street Catherine Ouest, Montreal, Quebec H3G 2W1, Canada. achandra@alcor.concordia.ca
authors.forename: Arvind
authors.initials: A
authors.lastname: Chandrasekaran
authors.forename: Muthukumaran
authors.initials: M
authors.lastname: Packirisamy
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000295:instrumentation*
chemical_list: D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase