Low level of O2 inhibits commitment of cultured mesenchymal stromal precursor cells from the adipose tissue in response to osteogenic stimuli.
Mesenchymal stromal precursor cells from human lipoaspirate (lMSC) cultured at 5% O(2) formed 50% less mineralized matrix in response to osteogenic induction than cells cultured under standard conditions (20% O(2)). After lMSC percultured at 5% O(2) were transferred to normoxic conditions (20% O(2)), they produced the same amount of matrix as lMSC permanently cultured at 20% O(2). Hence, hypoxia inhibited the commitment of lMSC under the effect of osteogenic stimuli, which can be important in reparative and regenerative medicine.
Grinakovskaya
O S
OS
Institute of Biomedical Problems, Russian Academy of Sciences, Moscow. grinakovskaya@imbp.ru
Andreeva
E R
ER
Buravkova
L B
LB
Rylova
Yu V
YV
Kosovsky
G Yu
GY
D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology*
D010100:Oxygen