Antibodies to endogenous tear protein in normal human tears.
OBJECTIVE
To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears.


METHODS
Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins.


RESULTS
Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins.


CONCLUSIONS
Normal human ocular tears contain antibodies to endogenous tear protein.
Remington
Susann G
SG
Department of Ophthalmology, HealthPartners Medical Group and Clinics, St Paul, Minnesota, USA. susann.g.remington@healthpartners.com
Crow
Jean M
JM
Nelson
J Daniel
JD
D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000276:immunology*; D054835:Lipocalin 1 / Q000276:immunology*; D009113:Muramidase / Q000276:immunology*; D013666:Tears / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D054835:Lipocalin 1; D009113:Muramidase; D007781:Lactoferrin