Exogenous sucrose utilization and starch biosynthesis among sweet potato cultivars.
Three sweetpotato cultivars were investigated for their starch content and amylose/amylopectin ratio. Ym starch contains 87.2% amylopectin and 12.8% amylose, when total starch was calculated as 100%. The Zm cultivar contains 33.6% amylopectin and 18.2% amylose, and its total starch was calculated as 51.8% of that of Ym. The Hm cultivar contains 39.1% amylopectin and 30.5% amylose, and its total starch was 69.6%. We analyzed the expression levels of starch and sucrose biosynthesis-related genes including AGPases a, b, and c; sucrose synthases I and II; starch synthase I; GBSS I; and SBEs I and II. All genes tested in this experiment were detected only in Ym, while several genes showed very faint or no expression in Zm and Hm. We also measured tissue-specific expression of these genes in whole plants of Ym. Most of the genes are expressed in the stem and roots of the plants. Expression profiles of starch synthesis-related genes of the sweetpotato leaves were investigated after supplementing the different concentrations of sucrose solution. All genes in Ym were clearly induced by sucrose, but the expression levels of some of these genes did not change in Zm and Hm. The total starch content of Ym, Zm, and Hm gradually increased over time on addition of 3%, 6%, and 9% sucrose concentrations. The greatest accumulation was observed in Ym at 48h, and it was almost 2.24 times higher than that of the (0%) control, while Zm and Hm showed 1.76 and 1.91 times higher levels of starch, respectively. These results indicate that cooperative expression of all related genes is essential for starch biosynthesis from sucrose. This is the first report on different sucrose contents and the efficiency with which exogenous sucrose switches on gene expression of starch biosynthesis-related genes among cultivars.
Ahn
Young Ock
YO
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Kim
Sun Ha
SH
Kim
Cha Young
CY
Lee
Joon-Seol
JS
Kwak
Sang-Soo
SS
Lee
Haeng-Soon
HS
D000687:Amylopectin / Q000378:metabolism; D000688:Amylose / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D027723:Ipomoea batatas / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013213:Starch / Q000096:biosynthesis* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism* / Q000494:pharmacology
D013395:Sucrose; D013213:Starch; D000688:Amylose; D000687:Amylopectin