title: [Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells].
abstract: OBJECTIVE
  To construct human Egr-1 promoter luciferase reporter system and study its activity induced by ionizing radiation.
  
  
  METHODS
  Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay.
  
  
  RESULTS
  The luciferase reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48 h after IR.
  
  
  CONCLUSIONS
  The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation. mediated gene therapy.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100850, China.
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Xu
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Ding
authors.forename: Ling-xue
authors.initials: LX
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Qin
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.forename: Qi-nong
authors.initials: QN
authors.lastname: Ye
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D051766:Early Growth Response Protein 1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D017930:Genes, Reporter / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011839:Radiation, Ionizing; D013997:Time Factors; D014162:Transfection
chemical_list: D051766:Early Growth Response Protein 1; D008156:Luciferases