Fiber evanescent wave spectroscopy using the mid-infrared provides useful fingerprints for metabolic profiling in humans.
Fiber evanescent wave spectroscopy (FEWS) explores the mid-infrared domain, providing information on functional chemical groups represented in the sample. Our goal is to evaluate whether spectral fingerprints obtained by FEWS might orientate clinical diagnosis. Serum samples from normal volunteers and from four groups of patients with metabolic abnormalities are analyzed by FEWS. These groups consist of iron overloaded genetic hemochromatosis (GH), iron depleted GH, cirrhosis, and dysmetabolic hepatosiderosis (DYSH). A partial least squares (PLS) logistic method is used in a training group to create a classification algorithm, thereafter applied to a test group. Patients with cirrhosis or DYSH, two groups exhibiting important metabolic disturbances, are clearly discriminated from control groups with AUROC values of 0.94+/-0.05 and 0.90+/-0.06, and sensibility/specificity of 8684% and 8787%, respectively. When pooling all groups, the PLS method contributes to discriminate controls, cirrhotic, and dysmetabolic patients. Our data demonstrate that metabolic profiling using infrared FEWS is a possible way to investigate metabolic alterations in patients.
Anne
Marie-Laure
ML
UMR-CNRS 6226, Campus de Beaulieu/Equipe Verres et Ceramiques, University of Rennes 1, Sciences chimiques de Rennes, Rennes, 35042, France.
Le Lan
Caroline
C
Monbet
Valérie
V
Boussard-Plédel
Catherine
C
Ropert
Martine
M
Sire
Olivier
O
Pouchard
Michel
M
Jard
Christine
C
Lucas
Jacques
J
Adam
Jean Luc
JL
Brissot
Pierre
P
Bureau
Bruno
B
Loréal
Olivier
O
D000293:Adolescent; D000328:Adult; D000368:Aged; D000465:Algorithms; D015415:Biomarkers / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D005260:Female; D005336:Fiber Optic Technology / Q000379:methods; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000097:blood* / Q000175:diagnosis*; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods*; D055815:Young Adult
D015415:Biomarkers