title: Induction of apoptosis in human colon cancer HCT-116 cells by anthocyanins through suppression of Akt and activation of p38-MAPK.
abstract: Anthocyanins belong to a class of flavonoids that exhibit important anti-oxidant and anti-inflammatory actions as well as chemotherapeutic effects. However, little is known concerning the molecular mechanisms by which these activities are exerted. In this study, we investigated the anthocyanins isolated from Vitis coignetiae Pulliat for their potential anti-proliferative and apoptotic effects on human colon cancer HCT-116 cells. These anthocyanins inhibited cell viability and induce apoptotic cell death of HCT-116 cells in a dose-dependent manner. The apoptotic cell death was caspase-dependent and the anthocyanins regulated anti-apoptotic proteins (IAPs). In addition, apoptosis was associated with activation of p38-MAPK and suppression of Akt. In conclusion, this study suggests that the anthocyanins isolated from Vitis coignetiae Pulliat induce apoptosis might at least in part through activating p38-MAPK and suppressing Akt in human colon cancer HCT-116 cells.
authors.affiliation: Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan 614-052, Korea.
authors.forename: Dong Yeok
authors.initials: DY
authors.lastname: Shin
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.forename: Jing Nan
authors.initials: JN
authors.lastname: Lu
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Kang
authors.forename: Chung Ho
authors.initials: CH
authors.lastname: Ryu
authors.forename: Gi Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Kang
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D045325:HCT116 Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry; D048051:p38 Mitogen-Activated Protein Kinases / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D010936:Plant Extracts; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases