A statistical change point model approach for the detection of DNA copy number variations in array CGH data.
Array comparative genomic hybridization (aCGH) provides a high-resolution and high-throughput technique for screening of copy number variations (CNVs) within the entire genome. This technique, compared to the conventional CGH, significantly improves the identification of chromosomal abnormalities. However, due to the random noise inherited in the imaging and hybridization process, identifying statistically significant DNA copy number changes in aCGH data is challenging. We propose a novel approach that uses the mean and variance change point model (MVCM) to detect CNVs or breakpoints in aCGH data sets. We derive an approximate p-value for the test statistic and also give the estimate of the locus of the DNA copy number change. We carry out simulation studies to evaluate the accuracy of the estimate and the p-value formulation. These simulation results show that the approach is effective in identifying copy number changes. The approach is also tested on fibroblast cancer cell line data, breast tumor cell line data, and breast cancer cell line aCGH data sets that are publicly available. Changes that have not been identified by the circular binary segmentation (CBS) method but are biologically verified are detected by our approach on these cell lines with higher sensitivity and specificity than CBS.
Chen
Jie
J
Department of Mathematics and Statistics, University of Missouri-Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA. chenj@umkc.edu
Wang
Yu-Ping
YP
D001943:Breast Neoplasms / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000378:metabolism; D018628:Gene Dosage; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome; D006801:Humans; D015233:Models, Statistical; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA
D004247:DNA