title: [Ru(bpy)2dppz]2+ electrochemiluminescence switch and its applications for DNA interaction study and label-free ATP aptasensor.
abstract: [Ru(bpy)2dppz]2+ electrochemiluminescence (ECL) was studied, and it was used to investigate DNA interaction and develop a label-free ATP aptasensor for the first time. ECL of [Ru(bpy)2dppz]2+ is negligible in aqueous solution, and increases approximately 1000 times when [Ru(bpy)2dppz]2+ intercalates into the nucleic acid structure. The ECL switch behavior of [Ru(bpy)2dppz]2+ is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. The ECL switch by DNA was applied to investigate the interaction of [Ru(bpy)2dppz]2+ with herring sperm DNA. The calculated equilibrium constant (K) is 1.35 x 10(6) M(-1), and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. Moreover, ATP can dramatically affect ECL of the [Ru(bpy)2dppz]2+/ATP aptamer complex. As a result, a label-free, sensitive, and selective [Ru(bpy)2dppz]2+ ECL method for ATP detection was developed. The detection limit is 100 nM for ATP (with a signal-to-noise ratio, S/N, of 3) with a linear range of 0-1 microM. The result demonstrates that [Ru(bpy)2dppz]2+ ECL holds great promise in aptasensors.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, People's Republic of China.
authors.forename: Lianzhe
authors.initials: L
authors.lastname: Hu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Bian
authors.forename: Haijuan
authors.initials: H
authors.lastname: Li
authors.forename: Shuang
authors.initials: S
authors.lastname: Han
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.forename: Lianxun
authors.initials: L
authors.lastname: Gao
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010619:Phenazines / Q000737:chemistry* / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D007364:Intercalating Agents; D009942:Organometallic Compounds; D010619:Phenazines; C077849:bis(2,2'-bipyridyl)(dipyrido(3,2-alpha-2',3'-c)phenazine)ruthenium (II); D000255:Adenosine Triphosphate; D004247:DNA