Brain ischemic preconditioning does not require PARP-1.
OBJECTIVE
Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in ischemic preconditioning of the heart and cultured neurons, but its role in brain ischemic preconditioning is unknown. Summary of Report- We report that 5-minute bilateral common carotid artery occlusion (BCCAO) in the mouse prompted reduction of infarct volumes triggered 24 hours later by 20-minute middle cerebral artery occlusion (MCAO). Pharmacological PARP-1 inhibition between BCCAO and MCAO did not impair preconditioning. The contents of the PARP-1 substrate NAD, those of its product poly(ADP-ribose), caspase-3 activation, and PARP-1 expression did not change after BCCAO within the preconditioned tissue. PARP-1 KO mice were similarly protected by the 5-minute BCCAO.


CONCLUSIONS
Data demonstrate that, at variance with the heart, PARP-1 is dispensable for brain ischemic preconditioning.
Faraco
Giuseppe
G
Department of Pharmacology, University of Florence, Italy.
Blasi
Francesco
F
Min
Wookee
W
Wang
Zhao-Qi
ZQ
Moroni
Flavio
F
Chiarugi
Alberto
A
D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology* / Q000517:prevention & control*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis*
C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases