title: Laboratory Maintenance of Bordetella pertussis.
abstract: The causative agent of the respiratory disease whooping cough, Bordetella pertussis, is a nutritionally fastidious microorganism but can be grown with relative ease in research laboratories. Stainer-Scholte synthetic broth medium and Bordet-Gengou blood agar both support growth of B. pertussis and are commonly used. B. pertussis prefers aerobic conditions and a temperature range of 35 degrees to 37 degrees C. Appropriate laboratory safety protocols are required to prevent the generation of aerosols, which could potentially spread this highly infectious agent.
authors.affiliation: California Polytechnic State University, San Luis Obispo, California, USA.
authors.forename: Robin R
authors.initials: RR
authors.lastname: Hulbert
authors.forename: Peggy A
authors.initials: PA
authors.lastname: Cotter
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D001886:Bordetella pertussis / Q000254:growth & development* / Q000302:isolation & purification; D003264:Containment of Biohazards; D003470:Culture Media / Q000737:chemistry; D005612:Freeze Drying / Q000379:methods; D006801:Humans; D014917:Whooping Cough / Q000382:microbiology
chemical_list: D003470:Culture Media