Microvascular reactivity in type 1 diabetics.
OBJECTIVE
To evaluate whether differences are present in microvascular response to the schemia induced by dynamic videocapillaroscopy (VCD), through analysis of the measured capillar transverse segment area (CTSA) in patients with type 1 diabetes mellitus (T1DM).


METHODS
The vascular reactivity of the CTSA was studied by VCD, using a reactive hyperemia test in 61 volunteers, being 31 healthy controls without diabetes family history (Group 1) and 30 patients with T1DM without complications (Group 2). The images were captured every two seconds, during reperfusion after one minute induced ischaemia, and they were analyzed by the program Studio Version 8 and Motic Image Plus. The pre-ischemia capillary transverse segment (basal area, BA), the maximum strain post-ischemia (maximum area, MA), and time to achieve it (MAt) were measured during reperfusion, and the increased area percentage (Ap) was estimated.


RESULTS
The mean differences between groups were evaluated by the t-test. The median comparisons between the groups were studied by the Mann-Whitney test. There was no difference in BA between the groups. The Ap was significantly lower among the diabetic patients, and there was a significant increase in the Mat among the patients of Group 2 when compared to Group 1.


CONCLUSIONS
These data suggest that type 1 diabetes provokes earlier endothelial dysfunction, before the onset of clinically detectable degenerative complications. The outcomes from these alterations need further studies.
Abi-Chahin
Tereza Cristina
TC
Departamento de Ciências Morfológicas, Faculdade de Medicina Souza Marques, Rio de Janeiro, RJ, Brasil.
Hausen
Moema de Alencar
Mde A
Mansano-Marques
Claudia Moraes
CM
Halfoun
Vera Lucia Rabello de Castro
VL
D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D002196:Capillaries / Q000503:physiopathology*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D003925:Diabetic Angiopathies / Q000175:diagnosis; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007511:Ischemia / Q000503:physiopathology; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D020678:Microscopic Angioscopy / Q000379:methods*; D018709:Statistics, Nonparametric; D055815:Young Adult