title: Interpretation of molecular dynamics on different time scales in unilamellar vesicles using field-cycling NMR relaxometry.
abstract: Fast field-cycling (FFC) and rotating-frame nuclear magnetic resonance relaxometry were used to study molecular and collective dynamics in unilamellar liposome systems. Relaxation data for liposomes of diameter about 100 nm composed of 1,2-dimyristoyl-sn-glycero-3-posphocholine (DMPC) or 1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC) were obtained. The Larmor frequency dependence of the spin-lattice relaxation rates was interpreted in terms of clearly defined relaxation mechanisms associated with the underlying molecular dynamics. The physical parameters obtained from the analysis are consistent with values available in the literature obtained from a range of experimental techniques. This work establishes the potential of our approach to study dynamics in liposomal samples of more complex lipid composition.
authors.affiliation: National Institute for Cellular Biotechnology, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
authors.forename: Carla J
authors.initials: CJ
authors.lastname: Meledandri
authors.forename: Josefina
authors.initials: J
authors.lastname: Perlo
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Farrher
authors.forename: Dermot F
authors.initials: DF
authors.lastname: Brougham
authors.forename: Esteban
authors.initials: E
authors.lastname: Anoardo
mesh_terms: D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D010713:Phosphatidylcholines / Q000737:chemistry; D013997:Time Factors; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; C017251:1,2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine