Memory B and T cell responses induced by serotype 4 Streptococcus pneumoniae vaccines: longitudinal analysis comparing responses elicited by free polysaccharide, conjugate and carrier.
We have conducted a 1-year longitudinal study in mice vaccinated by free serotype 4 Streptococcus pneumoniae PS (PS4), the corresponding tetanus toxoid (TT)-conjugated vaccine, or the TT carrier alone. B and T cell immunity induced by these three types of antigen, were compared by monitoring the (i) long-term persistence of specific serum antibodies, (ii) frequency of memory B cell precursors in spleen, and (iii) T cell responses against the carrier. While PS4-specific antibody response appeared later than the anti-carrier response upon primary immunization, PS4-specific B memory and serum responses were quantitatively and qualitatively similar to the ones observed against TT upon immunization by either the free carrier or the conjugate. We also observed a parallel persistent carrier-specific T cell response in the spleen. These data indicate that the nature and long-term kinetics of the anti-PS4 antibody response induced by the conjugate vaccine are similar to "classical" T-dependent response elicited by conventional protein antigens.
Deauvieau
Florence
F
Sanofi Pasteur, Discovery Department, 69280 Marcy L'Etoile, France. deauviea@lyon.fnclcc.fr
Dussurgey
Sebastien
S
Rossignol
Daniele
D
de Montfort
Aymeric
A
Burdin
Nicolas
N
Guy
Bruno
B
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology*
D001428:Bacterial Vaccines; D016207:Cytokines; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate