title: Effects of resin or charcoal treatment on fetal bovine serum and bovine calf serum.
abstract: BACKGROUND
  Charcoal- or resin-stripping of fetal bovine serum (FBS) or bovine calf serum (BCS) intended for supplementation of cell culture media is widely practiced to remove a variety of endogenous compounds, including steroid, peptide, and thyroid hormones. The possibility that stripping removes other biologically relevant factors from serum may not be appreciated.
  
  
  METHODS
  In this report, standardized clinical laboratory testing methods were used to assess the effects of resin- and charcoal-stripping on content in FBS and BCS of more than 25 analytes in the sera.
  
  
  CONCLUSIONS
  In addition to hormones, the serum constituents affected by stripping are certain vitamins, electrolytes, enzyme activities, and metabolites.
authors.affiliation: Laboratory of Molecular Diagnostics, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Cao
authors.forename: Clint
authors.initials: C
authors.lastname: West
authors.forename: Carol S
authors.initials: CS
authors.lastname: Norton-Wenzel
authors.forename: Robert
authors.initials: R
authors.lastname: Rej
authors.forename: Faith B
authors.initials: FB
authors.lastname: Davis
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Davis
authors.forename: Robert
authors.initials: R
authors.lastname: Rej
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards; D002606:Charcoal / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry / Q000592:standards; D004573:Electrolytes / Q000032:analysis / Q000302:isolation & purification; D020536:Enzyme Activators / Q000032:analysis / Q000302:isolation & purification; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D006728:Hormones / Q000032:analysis / Q000302:isolation & purification; D012117:Resins, Synthetic / Q000494:pharmacology*; D044967:Serum / Q000737:chemistry / Q000187:drug effects*; D014815:Vitamins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004573:Electrolytes; D020536:Enzyme Activators; D004798:Enzymes; D006728:Hormones; D012117:Resins, Synthetic; D014815:Vitamins; D002606:Charcoal