title: A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies.
abstract: A new methodology has been developed to produce sections as thin as 5 microm of stink bug (Heteroptera: Pentatomidae) mouthparts for morphological studies. Heads of the southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) were fixed on Bouin solution, and treated with EDTA (ethylenediamine tetraacetic acid 7%) in an ice bath within a microwave oven for 0.5, 1, 2, 3, 4, and 5 h. The chitin structures and labium soft parts were well preserved after 0.5h EDTA treatment. Longer times of exposure to EDTA and microwave irradiation caused damage to mouthpart structures.
authors.affiliation: Department of Zoology, Federal University of Paraná, P.O. Box 19020, Curitiba, PR, 81531-980, Brazil.
authors.forename: Rogério A
authors.initials: RA
authors.lastname: Depieri
authors.forename: Célia G T J
authors.initials: CG
authors.lastname: Andrade
authors.forename: Antônio R
authors.initials: AR
authors.lastname: Panizzi
mesh_terms: D000818:Animals; D004492:Edetic Acid; D006430:Hemiptera / Q000033:anatomy & histology*; D008872:Microwaves; D009055:Mouth / Q000033:anatomy & histology*; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D004492:Edetic Acid