Structural characterization of the transmembrane proximal region of the hepatitis C virus E1 glycoprotein.
A detailed knowledge of the mechanism of virus entry represents one of the most promising approaches to develop new therapeutic strategies. However, viral fusion is a very complex process involving fusion glycoproteins present on the viral envelope. In the two hepatitis C virus envelope proteins, E1 and E2, several membranotropic regions with a potential role in the fusion process have been identified. Among these, we have selected the 314-342 E1 region. Circular Dichroism data indicate that the peptide exhibits a clear propensity to adopt a helical folding in different membrane mimicking media, such as mixtures of water with fluorinated alcohols and phospholipids, with a slight preference for negative charged bilayers. The 3D structure of E1(314-342) peptide, calculated by 2D-NMR in a low-polarity environment, consists of two helical stretches encompassing residues 319-323 and 329-338 respectively. The peptide, presenting a largely apolar character, interacts with liposomes, as indicated by fluorescence and electron spin resonance spectra. The strength of the interaction and the deepness of peptide insertion in the phospholipid membrane are modulated by the bilayer composition, the interaction with anionic phospholipids being among the strongest ever observed. The presence of cholesterol also affects the peptide-bilayer interaction, favoring the peptide positioning close to the bilayer surface. Overall, the experimental data support the idea that this region of E1 might be involved in membrane destabilization and viral fusion; therefore it may represent a good target to develop anti-viral molecules.
Spadaccini
Roberta
R
Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Via Cintia, 80126 Napoli, Italy. rspadacc@unisannio.it
D'Errico
Gerardino
G
D'Alessio
Viviana
V
Notomista
Eugenio
E
Bianchi
Alessia
A
Merola
Marcello
M
Picone
Delia
D
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry*; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D006023:Glycoproteins / Q000737:chemistry*; D016174:Hepacivirus / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010713:Phosphatidylcholines / Q000378:metabolism; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013050:Spectrometry, Fluorescence; D013113:Spin Labels; D014364:Tryptophan / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000737:chemistry*
C087035:E1 protein, Hepatitis C virus; D006023:Glycoproteins; D010713:Phosphatidylcholines; D013113:Spin Labels; D014759:Viral Envelope Proteins; C002448:1,2-linoleoylphosphatidylcholine; D014364:Tryptophan