title: Hematopoiesis-related microRNA expression in myelodysplastic syndromes.
abstract: MicroRNAs (miRNAs) are negative regulators of expression of genes involved in hematopoiesis. The present study sought to link hematopoiesis-relevant miRNAs with myelodysplastic syndromes (MDS) and MDS progression to acute myeloid leukemia (AML). We assessed 25 mature miRNAs in total RNA from bone marrow (BM) and peripheral blood (PB) of 25 newly diagnosed patients with MDS and 12 controls. Twelve miRNAs in BM and six in PB were differentially expressed between patients with MDS and controls. Three of these miRNAs, belonging to the cluster 17-92, were overexpressed in both BM and PB. miR-15a in BM ( p = 0.034) and miR-16 in PB ( p = 0.005) were differentially expressed between low-risk and high-risk groups. miR-222 ( p = 0.0023) and miR-181a ( p = 0.014) expression was higher in AML than in MDS in both BM and PB. This study adds further evidence to the role of miRNAs in the pathogenesis of MDS and their transformation into AML.
authors.affiliation: Unit of Human Anatomy, Molecular Oncology Laboratory, School of Medicine, University of Barcelona, IDIBAPS, Barcelona, Spain.
authors.forename: Aina
authors.initials: A
authors.lastname: Pons
authors.forename: Benet
authors.initials: B
authors.lastname: Nomdedeu
authors.forename: Alfons
authors.initials: A
authors.lastname: Navarro
authors.forename: Anna
authors.initials: A
authors.lastname: Gaya
authors.forename: Bernat
authors.initials: B
authors.lastname: Gel
authors.forename: Tania
authors.initials: T
authors.lastname: Diaz
authors.forename: Sandra
authors.initials: S
authors.lastname: Valera
authors.forename: María
authors.initials: M
authors.lastname: Rozman
authors.forename: Mohamed
authors.initials: M
authors.lastname: Belkaid
authors.forename: Emili
authors.initials: E
authors.lastname: Montserrat
authors.forename: Mariano
authors.initials: M
authors.lastname: Monzo
mesh_terms: D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; C525710:MIR222, human; C540193:MIrn181 microRNA, human