title: Methanoculleus hydrogenitrophicus sp. nov., a methanogenic archaeon isolated from wetland soil.
abstract: An obligately anaerobic, methanogenic archaeon, strain HC(T), was isolated from soil of the Zoige wetland on the Tibetan plateau, China. The strain was isolated through construction of an artificial butyrate-degrading consortium in co-culture with a syntrophic bacterium, 'Syntrophomonas erecta subsp. sporosyntropha' JCM 13344. Cells of strain HC(T) were irregular coccoids, 0.8-2 mum in diameter, that occurred singly and utilized only H(2)/CO(2) for growth and methane production. Growth occurred at 18-45 degrees C (optimum around 37 degrees C). The pH for growth was 5.0-8.5 (optimal growth around pH 6.6). The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence analysis indicated that strain HC(T) was affiliated to the genus Methanoculleus, with sequence similarities of 94.8-97.2 % to existing members. However, strain HC(T) was distinguished from described Methanoculleus species by not using formate for growth or methane formation and not requiring acetate as a growth factor. On the basis of phylogenetic analysis and phenotypic characteristics, the novel species Methanoculleus hydrogenitrophicus sp. nov. is proposed, with strain HC(T) (=CGMCC 1.5146(T) =JCM 16311(T)) as the type strain.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.|Graduate University, Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Graduate University, Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Dong
mesh_terms: D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D019638:Methanomicrobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008697:Methane