Adipocyte culture medium stimulates invasiveness of MDA-MB-231 cell via CCL20 production.
We explored whether adipocyte culture medium affects the secreted chemokine profile of tumor cells, because adipocytes stimulate progression or metastasis of breast cancer cells, and chemokines secreted from tumor cells are involved in these processes. CCL20 expression was dramatically increased, and an NF-kappaB blocker completely inhibited adipocyte culture medium-induced CCL20 expression in MDA-MB-231 cells. We showed that adipocyte culture medium increased the production of TNF-alpha in MDA-MB-231 cells, which stimulated CCL20 expression in an autocrine fashion. Our data also showed that CCL20 increased the migration and invasiveness of MDA-MB-231 cells, but did not affect the proliferation of these cells.
Kim
Kun-Yong
KY
Department of Biological Science, Sookmyung Women's University, Seoul, Korea.
Baek
Ahmi
A
Park
Yun S
YS
Park
Mi Y
MY
Kim
Jae H
JH
Lim
Jong-Seok
JS
Lee
Myeong-Sok
MS
Yoon
Suk R
SR
Lee
Hee G
HG
Yoon
Yoosik
Y
Yoon
Do-Young
DY
Yang
Young
Y
D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054418:Chemokine CCL20 / Q000096:biosynthesis*; D018925:Chemokines / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D018450:Disease Progression; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C108355:CCL20 protein, human; D054418:Chemokine CCL20; D018925:Chemokines; D003470:Culture Media; D014409:Tumor Necrosis Factor-alpha