Immunomodulatory activity of IL-27 in human periapical lesions.
IL-27, a cytokine with pro-inflammatory and anti-inflammatory properties, is a new member of the IL-6/IL-12 family, whose function in periapical lesions is unknown. We hypothesized that the production of IL-27 and its effect depend upon the type of immune/inflammatory response and clinical presentation of periapical lesions. We tested this hypothesis by studying the expression and function of IL-27 in human periapical lesions, both in situ and in culture. Immunohistochemistry demonstrated the strongest expression of IL-27 by endothelial cells and mononuclear phagocytes. Its production by periapical lesion mononuclear cells (PL-MNC), especially in symptomatic lesions, was significantly higher compared with that in peripheral blood MNC and correlated with the frequency of CD14(+) and CD3(+) cells. Exogenous IL-27 stimulated Th1 and down-regulated Th17 cytokine production by PL-MNC from symptomatic lesions, but down-regulated Th1 and Th2 responses in asymptomatic lesions. These findings suggest that IL-27 is an immunomodulatory cytokine in periapical lesions, with complex biological effects.
Colić
M
M
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11002 Belgrade, Serbia. vmaimi@eunet.rs
Gazivoda
D
D
Majstorović
I
I
Dragicević
A
A
Vasilijić
S
S
Rudolf
R
R
Milosavljević
P
P
Vucević
D
D
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis / Q000276:immunology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000276:immunology; D042783:Endothelial Cells / Q000276:immunology; D006801:Humans; D056747:Immunomodulation / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D053583:Interleukin-1beta / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007378:Interleukins / Q000032:analysis / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D010483:Periapical Diseases / Q000097:blood / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology; D021122:Protein Subunits / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D055815:Young Adult
D017252:CD3 Complex; D020381:Interleukin-17; D053583:Interleukin-1beta; D015848:Interleukin-5; D007378:Interleukins; D018950:Lipopolysaccharide Receptors; C464565:MYDGF protein, human; D021122:Protein Subunits; D007371:Interferon-gamma