[Preparation technique of Cremastra appendiculata synthetic seed].
The protocorm-suspension-system of Cremastra appendiculata was founded by liquid-suspension culture. The factors to effect germination rate and seedling conversion rate of C. appendiculata synthetic seeds, such as synthetic coating materials, synthetic endosperm components, storing conditions and germination materials, etc. were studied. The result showed that the germination rate and seedling conversion rate of synthetic seeds were the highest on the MS solid-medium while using 4% sodium alginate + 2% CaCl2 + 2% chitosan as synthetic coating materials, with 1/2 MS liquid-medium + 0.2 mg x L(-1) NAA + 0.1 mg x L(-1) GA3 + 0.5 mg L(-1) BA + 0.4 mg x L(-1) penicillin + 10.0 mg x L(-1) endophyte extract +0.3% carbendazim powder + 0.2% sodium benzoate + 1.0% sucrose as synthetic endosperm. And the germination rate and seedling conversion rate of synthetic seeds could attain to 68% and 65% after 20 days storing at 4 degrees C. The germination rate and seedling conversion rate of synthetic seeds decreased to a great extent with increasing the storing temperature and prolonging storing time.
Zhang
Mingsheng
M
School of Life Sciences, Guizhou University, Guiyang 550025, China. mszhang@gzu.edu.cn
Peng
Siwen
S
Yang
Xiaorui
X
Xu
Li
L
D003470:Culture Media / Q000378:metabolism; D018525:Germination; D029595:Orchidaceae / Q000254:growth & development* / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media