title: [Cloning and analysis of promoter-active fragments from Corynebacterium glutamicum 10147].
abstract: OBJECTIVE
  To clone promoter-active fragments from Corynebacterium glutamicum for further construction of expression vectors.
  
  
  METHODS
  Random Sau3A I digested fragments of C. glutamicum 10147 chromosome were shot-gun cloned into the promoter-probe vector pAKC6 and promoter activity of the inserted fragments was selected by chloramphenicol resistance of transformed C. glutamicum cells.
  
  
  RESULTS
  Thirty promoter-carrying fragments were isolated. Three C. glutamicum clones harboring pAKC6 with promoter fragments displayed chloramphenicol acetyltransferase (CAT) activity of more than 24 U/mg. The fragment F57 led to the highest CAT activity of 32.50 U/mg, even more than that produced by the promoter Ptrc, 26.33 U/mg.
  
  
  CONCLUSIONS
  The strength of promoter on fragments F21, F54 and F57 is as strong as promoter Ptrc in C. glutamicum. These fragments can be used to construct expression vector.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. guimingl@126.com
authors.forename: Guiming
authors.initials: G
authors.lastname: Liu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiuyuan
authors.initials: J
authors.lastname: Ding
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D048230:Corynebacterium glutamicum / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic