title: Glycosyltransferases involved in the biosynthesis of the inner core region of different lipopolysaccharides.
abstract: The inner core of lipopolysaccharide (LPS) structures in Gram-negative bacteria is considered a highly conserved region. The sugar connecting the membrane-associated lipid A moiety with the hydrophilic saccharide moiety, 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (Kdo) is present in every LPS molecule investigated but it may be partially replaced by d-glycero-alpha-d-talo-oct-2-ulosonic acid (Ko). l-Glycero-alpha-d-manno-heptose (Hep) and phosphate residues are part of most but not all LPS structures and additionally, modifications with 4-amino-4-deoxy-beta-l-arabinose (Ara4N) residues occur in some. A number of different glycosyltransferases is involved in the biosynthesis of the inner core region of different lipopolysaccharides. Here, we report the characterization of Kdo transferases, heptosyltransferases and Ara4N transferases from a variety of bacteria.
authors.affiliation: Division of Medical and Biochemical Microbiology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany. sgr06@dsmz.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Gronow
authors.forename: Guoqing
authors.initials: G
authors.lastname: Xia
authors.forename: Helmut
authors.initials: H
authors.lastname: Brade
mesh_terms: D001089:Arabinose / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology; D002236:Carbohydrate Conformation; D057075:Enzyme Assays; D016695:Glycosyltransferases / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D013400:Sugar Acids / Q000737:chemistry / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D013400:Sugar Acids; D001089:Arabinose; D016695:Glycosyltransferases