e2f1 Gene is a new member of Wnt/beta-catenin/Tcf-regulated genes.
HDAC inhibitors induce cell cycle arrest of E1A+Ras-transformed cells accompanied by e2f1 gene down-regulation and activation of Wnt pathway. Here we show that e2f1 expression is regulated through the Wnt/Tcf-pathway: e2f1 promoter activity is inhibited by sodium butyrate (NaB) and by overexpression of beta-catenin/Tcf. The e2f1 promoter was found to contain two putative Tcf-binding elements: the proximal one competes well with canonical Tcf element in DNA-binding assay. Being inserted into luciferase reporter vector, the identified element provides positive transcriptional regulation in response to beta-catenin/Tcf co-transfection and NaB treatment. Thus we have firstly demonstrated that e2f1 belongs to genes regulated through Wnt/beta-catenin/Tcf pathway.
Abramova
Maria V
MV
Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av 4, 194064 St Petersburg, Russia. mav_2004@mail.ru
Zatulovskiy
Evgeny A
EA
Svetlikova
Svetlana B
SB
Kukushkin
Alexander N
AN
Pospelov
Valery A
VA
D000818:Animals; D001483:Base Sequence; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D050687:E2F1 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051820:TCF Transcription Factors / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
D002087:Butyrates; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D056572:Histone Deacetylase Inhibitors; D051820:TCF Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin