Piezo-adapted 3-isopropylmalate dehydrogenase of the obligate piezophile Shewanella benthica DB21MT-2 isolated from the 11,000-m depth of the Mariana Trench.
3-isopropylmalate dehydrogenase (IPMDH)-encoding leuB genes were obtained from the obligate piezophile Shewanella benthica DB21MT-2 and non-piezophile Shewanella oneidensis MR-1. The genes were expressed in Escherichia coli and the proteins were purified using His-tag. The estimated kinetic parameters of these enzymes indicated that IPMDH of S. benthica DB21MT-2 is more tolerant of high pressure than that of S. oneidensis MR-1. Thus such an adaptation is one of the mechanisms bacteria utilize for survival at high pressures.
Kasahara
Ryota
R
Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan.
Sato
Takako
T
Tamegai
Hideyuki
H
Kato
Chiaki
C
D050539:3-Isopropylmalate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000222:Adaptation, Physiological; D055162:Biocatalysis; D002846:Chromatography, Affinity; D004586:Electrophoresis; D006639:Histidine / Q000378:metabolism; D006874:Hydrostatic Pressure; D010140:Pacific Ocean; D020592:Shewanella / Q000201:enzymology* / Q000302:isolation & purification* / Q000502:physiology
D006639:Histidine; D050539:3-Isopropylmalate Dehydrogenase