title: Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source.
abstract: Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.
authors.affiliation: Shizuoka Prefectural Livestock Institute, Swine and Poultry Research Center, 2780 Nishikata, Kikugawa, Shizuoka 439-0037, Japan. kawarasaki@sp-exp.pref.shizuoka.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kawarasaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Uchiyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirao
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Azuma
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Otake
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Shibata
authors.forename: Seiko
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: Shin
authors.initials: S
authors.lastname: Enosawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Konno
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hakamata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshino
authors.forename: Takuya
authors.initials: T
authors.lastname: Wakai
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ookawara
authors.forename: Hozumi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D023421:Models, Animal; D009928:Organ Specificity; D013552:Swine / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D049452:Green Fluorescent Proteins