title: Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan.
abstract: Fluorescent-labeled invertase, a hyperglycosylated mannoprotein from Saccharomyces cerevisiae, was found to bind to Lactococcus lactis IL1403 at acidic pH. Proteins on the cell wall of the bacterium affinity-purified using invertase as a ligand were identified to be heat shock proteins such as DnaK and GroEL and glycolytic enzymes such as pyruvate kinase and glyceraldehyde-3-phosphate dehydrogenase. DnaK bound to both the bacterium and yeast at pH 4 and aggregated them at above 0.1 mg/ml, whereas no significant difference between the circular dichroism spectra of DnaK at pH 4 and 7 was observed. These results indicate that the cytosolic proteins, including DnaK displayed on the cell wall, cause the lactic acid bacterium to adhere to the yeast.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. katakura@bio.eng.osaka-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Katakura
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sano
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Suteaki
authors.initials: S
authors.lastname: Shioya
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism*; D001709:Biotechnology; D002473:Cell Wall / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D008351:Mannans; D008565:Membrane Proteins; D043324:beta-Fructofuranosidase