Lipids modulate H(2)S/HS(-) induced NO release from S-nitrosoglutathione.
Recently we observed that a gas messenger H(2)S/HS(-) released NO from S-nitrosoglutathione (Ondrias et al., Pflugers Arch. 457 (2008) 271-279). However, the effect of biological compounds on the release is not known. Measuring the NO oxidation product, which is nitrite, by the Griess reaction, we report that unsaturated fatty acid-linoleic acid and lipids having unsaturated fatty acids: asolectin, dioleoylphosphocholine and dioleoylphosphoserine depressed the H(2)S/HS(-) induced NO release from S-nitrosoglutathione. On the other hand, a depression effect of the saturated fatty acid-myristic acid and lipids having saturated fatty acids, dilauroylphosphatidylcholine, dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine and distearoylphosphatidylcholine was less pronounced. The inhibition effect increased with the decreasing gel-to-liquid phase transitions temperature of the fatty acids and lipids. We suggest that lipid composition of biological membranes modulates NO release from nitrosoglutathione induced by H(2)S/HS(-), assuming that a reaction of H(2)S/HS(-) with unsaturated bonds of fatty acids may be partially responsible for the effect.
Tomaskova
Zuzana
Z
Institute of Molecular Physiology and Genetics, Bratislava, Slovakia.
Cacanyiova
Sona
S
Benco
Andrej
A
Kristek
Frantisek
F
Dugovicova
Lea
L
Hrbac
Jan
J
Ondrias
Karol
K
D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D026422:S-Nitrosoglutathione / Q000378:metabolism*
D005227:Fatty Acids; D008563:Membrane Lipids; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D006862:Hydrogen Sulfide