title: A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains.
abstract: Endogenous retroviruses (ERVs) contribute to a range of germline, as well as somatic mutations in mammals. However, autonomous retrotransposition of potentially active elements has not been demonstrated in the rat genome. We cloned an insertion that disrupted the normal splicing of the Cntrob gene that was subsequently identified as a nonautonomous, novel endogenous retrovirus of the RnERV-K8e family. The RnERV-K8e family is closely related to the recently reported MmERV-K10c elements, but differs from the autonomous mouse MusD or IAP families. In addition, we identified a novel, unexpectedly close relative of RnERV-K8e in the mouse, suggesting ERV-K cross-species transmission between mice and rats. We cloned a potentially autonomous RnERV-K8e element identified by in silico analysis and, using an in vitro retrotransposition assay, demonstrated that it is capable of retrotransposition. This particular element (named Rat-rho, pronounced "retro") encodes a retroviral envelope gene (env); however, env is not required for de novo retrotransposition events. Significant levels of RnERV-K8e-associated genetic polymorphisms were detected among inbred rat strains, suggesting ongoing retrotransposition in the rat genome. This study identifies an ERV-K-type family in rats that shows obvious signs of recent activity. Ongoing retrotranspositional activity may significantly add to genomic variability among inbred rat strains.
authors.affiliation: Max-Delbrück-Center for Molecular Medicine (MDC), Berlin, Germany.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Wang
authors.forename: Frantisek
authors.initials: F
authors.lastname: Liska
authors.forename: Claudia
authors.initials: C
authors.lastname: Gosele
authors.forename: Lucie
authors.initials: L
authors.lastname: Sedová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kren
authors.forename: Drahomíra
authors.initials: D
authors.lastname: Krenová
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ivics
authors.forename: Norbert
authors.initials: N
authors.lastname: Hubner
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Izsvák
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics; D020077:Endogenous Retroviruses / Q000145:classification / Q000235:genetics; D005260:Female; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation; D016678:Genome / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics* / Q000821:virology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*; D016662:Virus Integration
chemical_list: D004251:DNA Transposable Elements; D014759:Viral Envelope Proteins