title: Antiaggregant effect of taurine chloramines in the presence of serum albumin.
abstract: The effects of taurine chloramine derivatives on initial aggregation of isolated platelets suspended in buffered saline were studied. Inhibition of ADP-induced aggregation in pure cell suspension depended on the structure of chloramine antiaggregants. The most effective of them was N,N-dichlorotaurine; its concentration needed for 50% inhibition of aggregation was about 0.1 mM. Weaker antiaggregants N-chloro-N-methyltaurine and N-chlorotaurine in a final concentration of 0.5 mM reduced platelet aggregation by only 10%. The studied chloramines considerably differed by their characteristics (velocity of the reaction with sulfur-containing groups of atoms). N,N-dichlorotaurine exhibited the weakest reactivity with methionine thioester group. In turn, the velocity constant with reduced glutathione was by 2-3 orders of magnitude higher than that of other chloramines. Antiaggregant effect of taurine chloramine derivatives was 2-fold higher in the presence of serum albumin, presumably due to special interactions of taurine chloramines in complex with albumin with platelets.
authors.affiliation: Institute of Physicochemical Medicine, Federal Agency for Health Care and Social Development, Moscow, Russia. marina_murina@mail.ru
authors.forename: M A
authors.initials: MA
authors.lastname: Murina
authors.forename: D I
authors.initials: DI
authors.lastname: Roshchupkin
authors.forename: N A
authors.initials: NA
authors.lastname: Chudina
authors.forename: A O
authors.initials: AO
authors.lastname: Petrova
authors.forename: V I
authors.initials: VI
authors.lastname: Sergienko
mesh_terms: D000818:Animals; D002700:Chloramines / Q000737:chemistry / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry* / Q000494:pharmacology*; D013654:Taurine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002700:Chloramines; C454660:N,N-dichlorotaurine; D010975:Platelet Aggregation Inhibitors; D012709:Serum Albumin; D013654:Taurine; C043410:N-chlorotaurine