[An improved method to recover high quality genomic DNA from paraffin-embedded tissues].
OBJECTIVE
To establish a high quality genomic DNA preparation method from formalin fixed and paraffin embedded tissues (FFPET) by integrating previous DNA extraction methods.


METHODS
We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection.


RESULTS
The improved genomic DNA extraction method combined the advantages of the water-bath de-waxing and DNA affinity column, making it possible to get high quality genomic DNA from paraffin embedded cervical cancer tissues, and especially efficient to recover genomic DNA fragments larger than 20 kb.


CONCLUSIONS
The improved DNA extraction method is fast and convenient to recover high quality genomic DNA from paraffin embedded tissues.
Yilike
Xiamixinuer
X
Department of Biology College of Preclinical Medicine, Xinjiang Medical University, Urumqi, China.
Yang
Xi
X
Wu
Guizhen
G
Ma
Qi
Q
Abudula
Abulizi
A
D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods; D016707:Tissue Fixation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D005557:Formaldehyde; D004247:DNA