title: Methylene tetrahydrofolate reductase and angiotensin converting enzyme gene polymorphisms related to overweight/obesity among Saudi subjects from Qassim Region.
abstract: BACKGROUND
  This work was planned to check for the association of polymorphisms related to methylenetetrahydrofolate reductase (MTHFR) and angiotensin converting enzyme (ACE) genes with overweight/obesity among Saudi subjects from Qassim region.
  
  
  METHODS
  This work included 130 subjects having overweight or obesity and 111 normal controls. Their age mean +/- SD was 27 +/- 9.8 and 24 +/- 8.8 years respectively. Their DNA was analyzed for polymorphisms of MTHFR; 677C/T and 1298 A/C and ACE; I/D genes using real-time PCR.
  
  
  RESULTS
  Genotype and allele frequencies of studied polymorphisms in cases of overweight/obesity showed no significant statistical difference compared to that of controls. However, on analysis of body mass index (BMI), cases showed slightly higher but statistically nonsignificant mean +/- SD values among those carrying the mutant MTHFR 677 T allele (CT + TT vs. CC, 30.7 +/- 4.5 vs. 29.9 +/- 4.9), 1298 C allele (AC + CC vs. AA, 29.9 +/- 4.1 vs. 29.7 +/- 5.5) and ACE D allele (ID + DD vs. II, 30.0 +/- 5.1 vs. 29.1 +/- 2.8). In addition controls having the DD and ID genotypes showed higher statistically significant values of BMI than those of the II genotype (22.0 +/- 1.9, 21.7 +/- 2.6 and 19.5 +/- 2.3 respectively, p < 0.05).
  
  
  CONCLUSIONS
  There is no solid association of polymorphisms related to MTHFR and ACE genes with non-complicated overweight or obesity among Saudi subjects from Qassim Region.
authors.affiliation: Molecular Biology Research Center, College of Medicine, Qassim University, Buraydah, Saudi Arabia. settin@mans.edu.eg
authors.forename: Ahmad A
authors.initials: AA
authors.lastname: Settin
authors.forename: Abdullah
authors.initials: A
authors.lastname: Algasham
authors.forename: Moataz
authors.initials: M
authors.lastname: Dowaidar
authors.forename: Hisham
authors.initials: H
authors.lastname: Ismail
mesh_terms: D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D009765:Obesity / Q000235:genetics*; D050177:Overweight / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors; D055815:Young Adult
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2); C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A