[Green fluorescent protein has no effect on blastocyst development in C57BL/6-Tg(ACTB-EGFP)1Osb/J mice].
The changes in the state of long-term culture of blastocysts derived from female C57BL/6 mice after crossing with C57BL/6-Tg(ACTB-EGFP)1Osb/J males with a green fluorescent protein transgene EGFP on chromosome 15 were studied. Possible causes of different culture results were analyzed: the preservation of undifferentiated cells as dense clusters in the inner cell mass or their differentiation into extraembryonic endoderm. Comparison of the events going in blastocysts with the -/- or -/EGFP genotypes demonstrated that the GFP presence has no effect on cell processes. This allows us to use embryonic material from this mouse line in experiments that require long-term vital observation of embryonic cells.
Sakharova
N Iu
NIu
Vikhliantseva
E F
EF
Smirnov
A A
AA
Konovalov
A N
AN
D000818:Animals; D001757:Blastomeres / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019076:Transgenes
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins