title: Mass spectrometric analysis of peptides in brain neurosecretory cells and neurohemal organs in the adult blowfly, Protophormia terraenovae.
abstract: Neuropeptides in neurosecretory cells of the pars intercerebralis (PI) and pars lateralis (PL) in the brain, and those in the corpus cardiacum-hypocerebral ganglion complex (CC-HG) and corpus allatum (CA) were examined by mass spectrometry and immunocytochemistry in adult females of the blowfly, Protophormia terraenovae. By using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and electrospray ionization quadrupole orthogonal acceleration time-of-flight mass spectrometry (ESI-Q-Tof MS) and MS/MS, 4 peptides (including myosuppressin and SIFamide) were detected in the PI, 12 peptides (including [Arg(7)]-corazonin and [Arg(7)]-corazonin(3)(-)(11)) in the PL, 13 peptides (including myosuppressin, [Arg(7)]-corazonin and [Arg(7)]-corazonin(3-11)) in the CC-HG, and 6 peptides in the CA. MALDI-TOF MS analysis of each tissue or organ was made in about 20 flies under diapause-inducing (LD 12:12 at 20 degrees C) and diapause-averting conditions (LD 18:6 at 25 degrees C). These molecular ion peaks did not distinctively differ between diapause-inducing and diapause-averting conditions. A peptide with an m/z value at 1395.1 was purified from 240 brains and the 2nd-10th amino acids were sequenced as -YRKPPFNGS-, corresponding to a partial sequence of SIFamide. Only two pairs of somata in the PI were immunoreactive to antisera against SIFamide, which were local neurons widely extending fibers throughout the brain neuropils.
authors.affiliation: Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Inosaki
authors.forename: Akikazu
authors.initials: A
authors.lastname: Yasuda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Shinada
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Ohfune
authors.forename: Hideharu
authors.initials: H
authors.lastname: Numata
authors.forename: Sakiko
authors.initials: S
authors.lastname: Shiga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D003335:Corpora Allata / Q000737:chemistry / Q000166:cytology; D004175:Diptera / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis*; D009490:Neurosecretory Systems / Q000737:chemistry* / Q000166:cytology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides; C060602:corazonin protein, insect