A new single-platform method for the enumeration of CD34+ cells.
Guidelines for flow cytometric enumeration of CD34(+) hematopoietic stem cells (HSC) recommend the use of a single-platform assay. The SCE kit has recently been commercialized by BD Biosciences. Results obtained with this newly available kit were compared with CD34(+) cell enumerations obtained in parallel with already commercialized diagnostic kits; fresh peripheral blood, apheresis, cord blood (CB) and bone marrow (BM) samples, as well as thawed apheresis and CB samples, were assayed. The SCE kit produced data for CD34(+) enumeration that correlate well with data produced with the older assays (r(2) > or = 0.9). Practical advantages were the ability to enumerate viable CD34 cells in all kinds of HSC products, the absence of bead pipetting (which decreases results precision) and a gating strategy complying with international recommendations. A major disadvantage was the absence of specific software for data analyses and presentation of results.
Lemarie
Claude
C
Centre de Thérapie Cellulaire et Génique, Institut Paoli-Calmettes, Marseille, France. lemariec@marseille.fnclcc.fr
Bouchet
Guillaume
G
Sielleur
Isabelle
I
Durand
Véronique
V
Navarro
Florent
F
Calmels
Boris
B
Chabannon
Christian
C
D018952:Antigens, CD34 / Q000378:metabolism; D002452:Cell Count / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D011933:Reagent Kits, Diagnostic
D018952:Antigens, CD34; D011933:Reagent Kits, Diagnostic