Rapid determination of the geographical origin of honey based on protein fingerprinting and barcoding using MALDI TOF MS.
The authentication of foods is an important aspect of quality control and food safety. Honey is one of the most natural and most popular foods in the world. A fast and reliable method to determine the geographical origin of honey was developed based on fingerprinting and barcoding of proteins in honey by using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) and MALDI Biotyper 1.1 software, respectively. The protein mass spectra of 16 honey samples of known Hawaii origin were obtained and peak information was extracted to generate protein fingerprints. This information was transformed into a database library of spectral barcodes that were used for differentiation of the geographical origin of honeys based on pattern matching. The differentiation ability of the database library of barcodes was validated by comparing the results of replicate assays of 5 of the 16 honey samples of known Hawaii origin obtained directly from the producers. Validation results showed that the protein fingerprints of honeys have better comparability with those honeys in the library known to be from the same region than with those of honey samples from other regions. The protein fingerprints were used to differentiate the geographical origins of commercially purchased honey samples with labels indicating that they were produced in different countries and various states of the USA, including Hawaii. The results showed that the MALDI TOF MS Biotyper system can be a rapid, simple and practical method for determining the geographical origin of honeys sold in commerce.
Wang
Jun
J
Department of Molecular Biosciences and Bioengineering, University of Hawaii of Manoa, Honolulu, Hawaii 96822, USA.
Kliks
Michael M
MM
Qu
Weiyue
W
Jun
Soojin
S
Shi
Gongyi
G
Li
Qing X
QX
D000818:Animals; D001516:Bees; D006254:Hawaii; D006722:Honey / Q000032:analysis*; D019476:Insect Proteins / Q000737:chemistry*; D010449:Peptide Mapping / Q000379:methods*; D011786:Quality Control; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014481:United States
D019476:Insect Proteins