title: Spatial control of the energy metabolism of yeast cells through electrolytic generation of oxygen.
abstract: The metabolic dynamics of yeast cells is controlled by electric pulses delivered through a spatially extended yeast cell/Au electrode interface. Concomitant with voltage pulses, oxygen is generated electrolytically at the electrode surface and delivered to the cells. The generation of oxygen was investigated in dependence of the applied voltage, width of the voltage pulses and temperature of the electrolytic solution. The local oxygen pulses at the electrodes lead to a transient activation of the aerobic energy metabolism of the yeast cells causing a perturbation in their energy balance. The effect of these local perturbations on the temporal dynamics of glycolysis in yeast cells is quantified in dependence of the energy state of cells.
authors.affiliation: Otto-von-Guericke Universität Magdeburg, Institut für Experimentelle Physik, Abteilung Halbleiterepitaxie, Universitätsplatz 2, 39106 Magdeburg, Germany. christian.warnke@ovgu.de
authors.forename: Christian
authors.initials: C
authors.lastname: Warnke
authors.forename: Thomas
authors.initials: T
authors.lastname: Mair
authors.forename: Hartmut
authors.initials: H
authors.lastname: Witte
authors.forename: Antje
authors.initials: A
authors.lastname: Reiher
authors.forename: Marcus J B
authors.initials: MJ
authors.lastname: Hauser
authors.forename: Alois
authors.initials: A
authors.lastname: Krost
mesh_terms: D004563:Electrochemistry; D004566:Electrodes; D004734:Energy Metabolism; D006046:Gold / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D013499:Surface Properties; D013696:Temperature
chemical_list: D006046:Gold; D010100:Oxygen