A homogeneous HLA-B*27 genotyping assay using dried reagent mixtures.
The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to start the homogenous amplification assay. The method was validated with 229 samples also typed with an existing DELFIA-based method and results of both assays were 100% concordant. The dried reagents were shown to be stable at least up to eight weeks at room temperature without any decline in their performance.
Kiviniemi
Minna
M
Immunogenetics Laboratory, University of Turku, FIN-20520 Turku, Finland. minna.kiviniemi@utu.fi
Ilonen
Jorma
J
Lövgren
Timo
T
D004247:DNA / Q000097:blood* / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D013167:Spondylitis, Ankylosing / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*
D015796:HLA-B27 Antigen; D011933:Reagent Kits, Diagnostic; D004247:DNA