title: Human immunodeficiency virus type 1 subtypes prevalence in central China.
abstract: OBJECTIVE
  To study the epidemic characteristics, transmission sources and routes of various subtypes of human immunodeficiency virus type 1 (HIV-1) and sequence variations in Henan, central China. To provide theoretical foundation for Acquired Immune Deficiency Syndrome (AIDS) prevention strategy in this region where the primary HIV transmission route was through former paid blood donation.
  
  
  METHODS
  HIV-1 gene env and gag were amplified by nested polymerase chain reaction (PCR) from uncultured peripheral blood mononuclear cells (PBMCs) obtained from 1,287 HIV-1 confirmed samples in Henan.
  
  
  RESULTS
  Among 1,287 samples, 5 HIV-1 strains were found including subtypes B' (95.9%), C (0.47%) and recombinant subtypes CRF 07_BC (1.09%), CRF 08_BC (1.79%) and CRF 01_AE (0.78%). Phylogenetic tree analysis found that 1,234 Henan subtype B' were closely related to those commonly found in Thailand, and were distantly related to other international subtypes. The dominant strain in former blood plasma donors (FPDs) was subtype B', and the dominant strains in sexual transmission were subtype B' and BC. Among HIV patients who were most likely infected through routes other than paid blood donation, the percentage of non-B' subtypes was much higher than those of FPD.
  
  
  CONCLUSIONS
  These findings suggest that the prevailing strain of HIV-1 in Henan is subtype B', similar to the B' subtype found in Thailand. In addition, for the first time we found subtypes C and recombinant subtypes CRF07_BC, CRF08_BC and CRF01_AE in this region. Indicating that the subtype feature of HIV-1 became more complicated than before in central China.
authors.affiliation: School of Public Health, Zhengzhou University, Zhengzhou, China. zhaofeicute@yahoo.com.cn
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D015658:HIV Infections / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D010802:Phylogeny; D015995:Prevalence; D013785:Thailand