Neolignans from Piper kadsura and their anti-neuroinflammatory activity.
Bioassay-guided column chromatographic separation of the methanolic extract of dried aerial parts of Piper kadsura (Piperaceae) led to the isolation of a new neolignan, piperkadsin C (1), together with eight known neolignans (2-9). The structures of the isolated compounds were elucidated by combined spectroscopic methods. The anti-neuroinflammatory activities of these compounds were evaluated by assessing nitric oxide (NO) production in LPS-activated BV-2 cells, a microglia cell line. Piperkadsin C (1) and futoquinol (2) potently inhibited NO production with an IC(50) value of 14.6 and 16.8microM in microglia cells, respectively. Compounds 3, 4, 5, 8, and 9 also exhibited moderate inhibition of NO production in BV-2 cells.
Kim
Ki Hyun
KH
Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 440-746, Republic of Korea.
Choi
Jung Wook
JW
Ha
Sang Keun
SK
Kim
Sun Yeou
SY
Lee
Kang Ro
KR
D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D009474:Neurons / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D017705:Lignans; D008070:Lipopolysaccharides; D010936:Plant Extracts; C549141:futoquinol; C549140:piperkadsin C; D009569:Nitric Oxide