Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis.
Zinc (Zn) is an essential micronutrient for various physiological and metabolic processes in plants, although it is toxic in excess. To understand better Zn-responsive proteins, we developed a highly improved method of isoelectric focusing (IEF) in which whole lysate from Arabidopsis roots is subjected to IEF without any desalting steps. In this method, samples extracted with lysis buffer containing 1.5% SDS can also be directly applied to IEF. By applying this method to Zn-treated Arabidopsis roots, 10 up-regulated and 17 down-regulated proteins were identified, 15 of which showed a significant correlation with previously reported transcriptomic data.
Fukao
Yoichiro
Y
Plant Science Education Unit, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101, Japan. f ukao@bs.naist.jp
Ferjani
Ali
A
Fujiwara
Masayuki
M
Nishimori
Yuka
Y
Ohtsu
Iwao
I
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D029681:Arabidopsis Proteins; D003470:Culture Media; D015032:Zinc