Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.
A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.
Usovetskii
I A
IA
Russian State Medical University, Moscow, Russia.
Burunova
V V
VV
Kovtun
N E
NE
Suzdal'tseva
Yu G
YG
Krasil'nikova
Yu B
YB
Korotkii
N G
NG
Yarygin
K N
KN
D001706:Biopsy; D018920:Coculture Techniques; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008544:Melanocytes / Q000166:cytology*; D012880:Skin Pigmentation; D014820:Vitiligo / Q000473:pathology*