[Effect of total flavonoids of jiawei wuzi yanzong prescription on VGCCs induced by amyloid-beta25-35 peptide in CA1 pyramidal neurons of rat hippocampal slice].
OBJECTIVE
To explore the effect of the total flavonoids of Jiawei Wuzi Yanzong prescription on the Voltage-gated calcium channel of the CA1 pyramidal cell of rat hippocampus.


METHODS
The inward calcium current was recorded by the whole-cell patch clamp and the amplitude of it was thought to observe the effect of Abeta25-35 and the total flavonoids. The hippocampus of rat was separated and cut into slices. Active pyramidal cells of slices were chosen for the whole-cell patch clamp recording. After exposure to Abeta25-35, voltage steps (500 ms) were used to depolarize stepwise in a range from 50 to +50 mV (increment: 5 mV). An inward Ca2+ current which was suggested to be survey was evoked. Application of the total flavonoids was to be observed if it had effect on this voltage-depended inward current.


RESULTS
Abeta25-35 could enhance the calcium current to induce intracellular calcium overload. The amplitude of the control group was--(157.1 +/- 19.9) pA, but after application of Abeta25-35 the current enhanced to--(323.2 +/- 23.4) pA. When the total flavonoids at concentration of 125, 250, 500 g x L(-1) were added, the current declined to--(257.9 +/- 31.6), - (196.4 +/- 29.8) and--(169.3 +/- 34.0) pA, respectively.


CONCLUSIONS
Abeta-induced intracellular calcium overload may be one of neurotoxic of beta-amyloid peptide. The total flavonoids of Jiawei Wuzi Yanzong prescription can suppress inward calcium current to protect neurons.
Li
Li
L
Department of Integrated Chinese and Western Medicine, First Hospital, Peking University, Beijing 100034, China.
Cai
Haoran
H
Li
Lin
L
Wang
Xuemei
X
D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004594:Electrophysiology; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D055553:Prescription Drugs / Q000737:chemistry / Q000494:pharmacology; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D016229:Amyloid beta-Peptides; D015220:Calcium Channels; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010446:Peptide Fragments; D055553:Prescription Drugs; C074636:amyloid beta-protein (25-35)