Analysis of the urine proteome of human contrast-induced kidney injury using two-dimensional fluorescence differential gel electrophoresis/matrix-assisted laser desorption time-of-flight mass spectrometry/liquid chromatography mass spectrometry.
BACKGROUND
The pathogenesis of contrast-induced nephropathy (CIN) remains unclear and is defined by changes in serum creatinine which is not a sensitive biomarker for acute kidney injury. Search for differentially expressed urinary protein or peptide could contribute to further understanding of the disease and may provide new biomarkers.


METHODS
This is a small sample research. Urine samples were obtained from patients who underwent percutaneous coronary intervention and were labeled with 3 different fluorescent dyes. After 2-dimensional electrophoresis was run, the differentially regulated spots were picked out and identified by mass spectrometry. Another 31 patients were used as validation group.


RESULTS
Among 56 significantly changed spots, mannose-binding lectin (MBL) and MBL-associated serine protease 2 were both significantly upregulated. Compared to the baseline value, urinary MBL was significantly increased in the CIN group (2.08, 1.42-5.72, vs. 1.09, 0.516-1.411; p < 0.01). Postprocedure urinary MBL in the CIN group was also significantly higher than that in the non-CIN group (2.08, 1.42-5.72, vs. 1.057, 0.738-1.885; p < 0.05).


CONCLUSIONS
The studies suggested that MBL may be associated with contrast-induced acute kidney injury. It leads to an attempt to define a new pathogenesis and a novel biomarker for CIN.
Wang
Ling
L
Renal Division, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Ni
Zhaohui
Z
Xie
Zhengsheng
Z
Yang
Fuquan
F
He
Ben
B
Liu
Jianping
J
Dai
Huili
H
Qian
Jiaqi
J
Jia
Minghong
M
D000368:Aged; D003287:Contrast Media / Q000009:adverse effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced / Q000652:urine*; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003287:Contrast Media; D020543:Proteome