Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats.
Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P=0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P=0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury.
Hirose
Takuo
T
Department of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Sciences and Medicine, 6-3 Aramaki-aza-Aoba, Aoba-ku, Sendai 980-8578, Japan. hirose-t@m.tains.tohoku.ac.jp
Mori
Nobuyoshi
N
Totsune
Kazuhito
K
Morimoto
Ryo
R
Maejima
Takahiro
T
Kawamura
Takuya
T
Metoki
Hirohito
H
Asayama
Kei
K
Kikuya
Masahiro
M
Ohkubo
Takayoshi
T
Kohzuki
Masahiro
M
Takahashi
Kazuhiro
K
Imai
Yutaka
Y
D000818:Animals; D005786:Gene Expression Regulation; D007678:Kidney Glomerulus / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009392:Nephrectomy; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011921:Rats, Inbred WKY; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D012083:Renin / Q000378:metabolism
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D012083:Renin