HNE-protein adducts formation in different pre-carcinogenic stages of hepatitis in LEC rats.
Lipid peroxidation is highly associated with chronic degenerative diseases such as cancer. 4-hydroxy-2-nonenal is one of the major products of lipid peroxidation. 4-hydroxy-2-nonenal can interact with biomolecules, changing their conformation and activity. This study presents 4-hydroxy-2-nonenal-protein adducts formation in the first stages of Long-Evans Cinnamon rat hepatitis, a well recognized model for oxidative stress-associated hepatocarcinogenesis. 4-hydroxy-2-nonenal-protein adducts appeared in hepatocyte cytoplasm before the beginning of hepatitis and their presence was very strong during hepatitis, while a transient perinuclear expression of 4-hydroxy-2-nonenal-protein adducts was shown mainly at early hepatitis stages. 4-hydroxy-2-nonenal-protein adducts formation correlated to the expression of the tumour marker glutathione S-transferase P-form. These results show that lipid peroxidation modification of proteins might be implicated in the first stages of hepatocyte cancer initiation in Long-Evans Cinnamon rats.
Marquez-Quiñones
Adriana
A
INRA-ENVT, UMR 1089-Xénobiotiques, 31931 Toulouse, France.
Cipak
Ana
A
Zarkovic
Kamelija
K
Fattel-Fazenda
Samia
S
Villa-Treviño
Saul
S
Waeg
Georg
G
Zarkovic
Neven
N
Guéraud
Françoise
F
D000208:Acute Disease; D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000378:metabolism*; D015227:Lipid Peroxidation; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D010396:Penicillamine; D011230:Precancerous Conditions / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D020332:Rats, Inbred LEC
D000447:Aldehydes; D011506:Proteins; D010396:Penicillamine; C027576:4-hydroxy-2-nonenal