title: Campylobacter concisus and other Campylobacter species in children with newly diagnosed Crohn's disease.
abstract: BACKGROUND
  Campylobacter concisus and other members of the Campylobacter genus have recently been suggested as possible etiological agents of Crohn's disease (CD). To further investigate this issue we determined the prevalence of these organisms in pediatric patients newly diagnosed with CD.
  
  
  METHODS
  DNA was extracted from fecal specimens collected from 54 children with CD, 27 noninflammatory bowel disease (non-IBD), and 33 healthy controls and subjected to polymerase chain reaction (PCR) sequencing.
  
  
  RESULTS
  Detection of C. concisus DNA using a newly developed PCR assay targeting the 16S rRNA gene of C. concisus showed that 65% (35/54) of fecal samples from CD children were positive, a prevalence significantly higher than that in the healthy (33%, 11/33, P = 0.008) and non-IBD controls (37%, 10/27, P = 0.03). The prevalence of all Campylobacter DNA using genus-specific primers in children with CD was 72% (39/54), which was significantly higher than the 30% (10/33, P = 0.0002) and 30% (8/27, P = 0.0003) observed in healthy and non-IBD controls, respectively.
  
  
  CONCLUSIONS
  Given the strengthening evidence for a significantly higher prevalence of C. concisus and other non-jejuni Campylobacter species in pediatric CD, investigation into the role of these non-jejuni Campylobacter species in the initiation of human IBD is clearly a priority.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Si Ming
authors.initials: SM
authors.lastname: Man
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Day
authors.forename: Steven T
authors.initials: ST
authors.lastname: Leach
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Lemberg
authors.forename: Hazel
authors.initials: H
authors.lastname: Mitchell
mesh_terms: D000293:Adolescent; D002167:Campylobacter / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000150:complications* / Q000175:diagnosis* / Q000235:genetics; D002648:Child; D003424:Crohn Disease / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S