Indirect- and direct-acting mutagenicity of diesel, coal and wood burning-derived particulates and contribution of polycyclic aromatic hydrocarbons and nitropolycyclic aromatic hydrocarbons.
Particulates exhausted from two types of diesel engines (DEPs), burning-derived particulates from three types of coal (CBPs) and burning-derived particulates from three types of wood (WBPs) were separated into four fractions by silica-gel column chromatography using n-hexane, n-hexane-dichloromethane (3:1, v/v), dichloromethane and methanol, as the corresponding eluents. The indirect-acting mutagenicity of each fraction was assayed by the Ames test using the Salmonella typhimurium TA100 strain with S9 mix and the direct-acting mutagenicity was assayed using the S. typhimurium TA98 strain without S9 mix. The polycyclic aromatic hydrocarbons (PAHs) and nitropolycyclic aromatic hydrocarbons (NPAHs) of each fraction were determined by high-performance liquid chromatography (HPLC). Both direct- and indirect-acting of mutagenicities were the highest in samples of DEPs. The contributions of PAHs in samples of WBPs and NPAHs in DEPs were the largest, respectively.
Yang
Xiao-Yang
XY
Atmospheric Chemistry & Aerosol Division, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. yangxy@craes.org.cn
Igarashi
Kazuhiko
K
Tang
Ning
N
Lin
Jin-Ming
JM
Wang
Wei
W
Kameda
Takayuki
T
Toriba
Akira
A
Hayakawa
Kazuichi
K
D000393:Air Pollutants / Q000633:toxicity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003031:Coal / Q000633:toxicity*; D005742:Gasoline / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D014934:Wood / Q000633:toxicity*
D000393:Air Pollutants; D003031:Coal; D005742:Gasoline; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons