title: Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with açai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay.
abstract: Açai, the fruit of a palm native to the Amazonian basin, is widely distributed in northern South America, where it has considerable economic importance. Whereas individual polyphenolics compounds in açai have been extensively evaluated, studies of the intact fruit and its biological properties are lacking. Therefore, the present study was undertaken to investigate the in vivo genotoxicity of açai and its possible antigenotoxicity on doxorubicin (DXR)-induced DNA damage. The açai pulp doses selected were 3.33, 10.0 and 16.67g/kg b.w. administered by gavage alone or prior to DXR (16mg/kg b.w.) administered by intraperitoneal injection. Swiss albino mice were distributed in eight groups for acute treatment with açai pulp (24h) and eight groups for subacute treatment (daily for 14 consecutive days) before euthanasia. The negative control groups were treated in a similar way. The results of chemical analysis suggested the presence of carotenoids, anthocyanins, phenolic, and flavonoids in açai pulp. The endpoints analyzed were micronucleus induction in bone marrow and peripheral blood cells polychromatic erythrocytes, and DNA damage in peripheral blood, liver and kidney cells assessed using the alkaline (pH >13) comet assay. There were no statistically significant differences (p>0.05) between the negative control and the groups treated with the three doses of açai pulp alone in all endpoints analyzed, demonstrating the absence of genotoxic effects. The protective effects of açai pulp were observed in both acute and subacute treatments, when administered prior to DXR. In general, subacute treatment provided greater efficiency in protecting against DXR-induced DNA damage in liver and kidney cells. These protective effects can be explained as the result of the phytochemicals present in açai pulp. These results will be applied to the developmental of food with functional characteristics, as well as to explore the characteristics of açai as a health promoter.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Juliana Carvalho
authors.initials: JC
authors.lastname: Ribeiro
authors.forename: Lusânia Maria Greggi
authors.initials: LM
authors.lastname: Antunes
authors.forename: Alexandre Ferro
authors.initials: AF
authors.lastname: Aissa
authors.forename: Joana D'arc Castania
authors.initials: JD
authors.lastname: Darin
authors.forename: Veridiana Vera
authors.initials: VV
authors.lastname: De Rosso
authors.forename: Adriana Zerlotti
authors.initials: AZ
authors.lastname: Mercadante
authors.forename: Maria de Lourdes Pires
authors.initials: Mde L
authors.lastname: Bianchi
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology; D028023:Arecaceae / Q000737:chemistry*; D001853:Bone Marrow / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D010936:Plant Extracts; D004317:Doxorubicin