[A indirect competitive ELISA to detect domoic acid in seawater and shellfish].
OBJECTIVE
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for measurent domoic acid (DA) in seawater and shellfish.


METHODS
DA was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) by carbodiimide reaction. DA-BSA as immunogen was injected in BALB/c mice. Titres of the antisera against DA were determinated using DA-OVA as coating ligand by ELISA method. After female BALB/c mice were immunized six times, the polyclonal antibodies anti-DA was obtained. The competitive indirect ELISA for domoic acid in shellfish and seawater was established.


RESULTS
Under optimal condition, the detection limit of DA was 10.0 ng/ml (equal 4 microg/g shellfish meat). The recovery of domoic acid added in seawater was 83.2% - 124.7% with a coefficient of variation of 4.7% - 5.9%, the recovery of domoic acid added in shellfish was 85.9% - 99.9% with a coefficient of variation of 2.4% - 7.1%.
Liu
Renyan
R
National Marine Environmental Monitoring Center, Dalian 116023, China. ryliu@nmemc.gov.cn
Xu
Daoyan
D
Dong
Yuhua
Y
Liang
Yubo
Y
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009498:Neurotoxins / Q000032:analysis; D012623:Seawater / Q000032:analysis; D012758:Shellfish / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008387:Marine Toxins; D009498:Neurotoxins; D014874:Water Pollutants, Chemical; C012301:domoic acid; D007608:Kainic Acid