Effect of recombinant feline interferon-omega alone and in combination with chemotherapeutic agents on putative tumour-initiating cells and daughter cells derived from canine and feline mammary tumours.
Interferons (IFNs) are naturally produced cytokines with multiple important biological functions. The activity of recombinant feline IFN-omega (rFeIFN-omega) alone and in combination with chemotherapeutic drugs was tested on canine and feline mammary carcinoma cell lines (REM134 and CAT-MT) and putative tumour-initiating cells (TIC) derived from these cell lines by sphere assay. Viability was measured by chemoluminescence and a one-way analysis of variance and Student's t-tests were used for statistical analysis. rFeIFN-omega showed in vitro antitumour activity on feline and canine mammary carcinoma cells and putative TICs with a dose-dependent and target cell-specific action. Putative TICs were more resistant to the action of rFeIFN-omega compared with daughter REM134 and CAT-MT cells. REM134 cells and TICs were more sensitive to rFeIFN-omega compared with the feline counterparts. An additive effect was noticed between rFeIFN-omega and conventional anticancer drugs, in particular following co-culture of cells with anthracycline drugs. The results suggest that rFeIFN-omega warrants further investigation as a therapeutic adjunct in feline and canine mammary tumours.
Penzo
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The Royal (Dick) School of Veterinary Studies and the Roslin Insitute, Hospital for Small Animals, The University of Edinburgh Easter Bush, Midlothian, EH25 9RG, UK.
Ross
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Muirhead
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Else
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Argyle
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D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002371:Cat Diseases / Q000188:drug therapy; D002415:Cats; D002478:Cells, Cultured; D004283:Dog Diseases / Q000188:drug therapy; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000662:veterinary; D005260:Female; D007370:Interferon Type I / Q000378:metabolism / Q000494:pharmacology*; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy*; D011994:Recombinant Proteins
D000970:Antineoplastic Agents; D007370:Interferon Type I; D011994:Recombinant Proteins; C063743:interferon omega 1