Clitocybin D, a novel human neutrophil elastase inhibitor from the culture broth of Clitocybe aurantiaca.
Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significant HNE inhibitory activity with an IC(50) value of 17.8 micronM.
Kim
Young-Hee
YH
Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
Ryoo
In-Ja
IJ
Choo
Soo-Jin
SJ
Xu
Guang-Hua
GH
Lee
Sangku
S
Seok
Soon-Ja
SJ
Bae
KiHwan
K
Yoo
Ick-Dong
ID
D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D054833:Isoindoles / Q000737:chemistry / Q000302:isolation & purification*; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011485:Protein Binding; D053491:Proteinase Inhibitory Proteins, Secretory / Q000737:chemistry / Q000302:isolation & purification*
D003470:Culture Media; D054833:Isoindoles; D053491:Proteinase Inhibitory Proteins, Secretory; C546745:clitocybin D; D019272:Leukocyte Elastase