The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella.
The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenopharyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney.
Xu
Qiao Q
QQ
College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
Chang
Ming X
MX
Xiao
Fan S
FS
Huang
Bei
B
Nie
Pin
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D007113:Immunity, Innate; D050835:Interferon Regulatory Factors / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D007668:Kidney / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012087:Reoviridae / Q000276:immunology / Q000472:pathogenicity; D012088:Reoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000276:immunology
D017931:DNA Primers; D018076:DNA, Complementary; D029941:Fish Proteins; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger