[Rescue and identification foot-and-mouth disease virus Asia1/JS/China/2005 strain with Arg-Gly-Asp RGD receptor recognition site].
OBJECTIVE
To construct an infectious full-length cDNA clone of Asial/JS/China/2005 strain with Arg-Gly-Asp (RGD) receptor recognition site.


METHODS
We constructed foot-and-mouth disease virus type Asial full-length cDNA clone pFMDV-RGD by using site-directed mutagenesis. The plasmid pFMDV-RGD contained the desired mutation. The plasmids pFMDV-RGD were linearized with NotI enzyme. Linearized plasmid and pcDNAT7P plasmids expressing T7 RNA polymerase cotransfected into BHK-21 cells to rescue FMDV-RGD.


RESULTS
We constructed FMDV Asial/JS/China/2005 strain full-length cDNA clone with Arg-Gly-Asp receptor recognition site by sequence. We obtained rescued virus by plasmid cotransfection. The results of sequencing, indirect immunofluorescence, electron microscope and sulk mice pathogenicity analysis showed foot-and-mouth disease virus containing Arg-Gly-Asp receptor recognition site was successfully rescued.


CONCLUSIONS
The results lay a foundation for further study of biology characteristic diversity of rescued virus with Arg-Gly-Asp and Arg-Asp-Asp (RDD) receptor recognition site.
Li
Pinghua
P
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 30046, China. xiaoxiaoyezi510@sohu.com
Bai
Xingwen
X
Cao
Weijun
W
Lu
Zengjun
Z
Sun
Pu
P
Yin
Hong
H
Liu
Zaixin
Z
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005536:Foot-and-Mouth Disease / Q000378:metabolism / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D051379:Mice; D011485:Protein Binding; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D011991:Receptors, Virus