Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells.
The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40 degrees C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.
Wei
Zheng-Li
ZL
Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan.
Ogawa
Ryohei
R
Takasaki
Ichiro
I
Zhao
Qing-Li
QL
Zheng
Hua-Chuan
HC
Ahmed
Kanwal
K
Hassan
Mariame A
MA
Kondo
Takashi
T
D003911:Dextrans / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D045325:HCT116 Cells; D006367:HeLa Cells; D018869:Heat-Shock Response; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods*
D003911:Dextrans; D012333:RNA, Messenger; C015219:fluorescein isothiocyanate dextran; D008156:Luciferases; D016650:Fluorescein-5-isothiocyanate