title: [Anaphylactic reactions in WZS minipig orally induced by glycinin].
abstract: OBJECTIVE
  To explore the suitable dosage and observation period of anaphylactic reactions in WZS minipigs induced by glycinin (11S).
  
  
  METHODS
  Twelve 45-day-old WZS minipigs were randomly divided into three groups (control group, 4% 11S group and 8% 11S group, n = 4), which were respectively orally sensitized (0 - 10d) and orally challenged (16 - 18d, 31d) with diets containing 0%, 4% 11S or 8% 11S soybean proteins to induce anaphylactic reactions. Clinical symptoms, skin prick reactions, serum IgG, serum IgE, histamine, mast cell in the small intestine were observed or measured.
  
  
  RESULTS
  Diarrhea and positive skin prick reactions at different degrees were observed in 11S groups. Compared with the control group, serum IgG, IgE and histamine levels of 4% 11S group at 19d and 32d were significantly higher (P < 0.05), and reached the peak when 11S groups were orally challenged at 19d. The jejunum histamine level was negatively correlated with the mast cell numbers in the jejunum mucosa and sub-mucosa (P < 0.05). Compared with the control, the mast cell number in the jejunum sub-mucosa of 4% 11S group was decreased (P < 0.05).
  
  
  CONCLUSIONS
  The WZS minipig allergic reaction orally induced by glycinin could be type I hypersensitivity mediated by IgE, reaction induced by 4% dosage glycinin could be more strong than that of 8% dosage, and stimulating phase could be a suitable observation period for anaphylactic reactions which could be related with the activation of mast cells in the small intestine.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. huangqiong18@yahoo.com.cn
authors.forename: Qiong
authors.initials: Q
authors.lastname: Huang
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Haixia
authors.initials: H
authors.lastname: Sui
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Feng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhi
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D005512:Food Hypersensitivity / Q000276:immunology*; D005916:Globulins / Q000008:administration & dosage / Q000009:adverse effects*; D007073:Immunoglobulin E / Q000097:blood; D007583:Jejunum / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D011897:Random Allocation; D030262:Soybean Proteins / Q000008:administration & dosage / Q000009:adverse effects*; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics; D013552:Swine; D013556:Swine, Miniature
chemical_list: D005916:Globulins; D030262:Soybean Proteins; D007073:Immunoglobulin E; C011474:glycinin