Adult generation of glutamatergic olfactory bulb interneurons.
The adult mouse subependymal zone (SEZ) harbors neural stem cells that are thought to exclusively generate GABAergic interneurons of the olfactory bulb. We examined the adult generation of glutamatergic juxtaglomerular neurons, which had dendritic arborizations that projected into adjacent glomeruli, identifying them as short-axon cells. Fate mapping revealed that these originate from Neurog2- and Tbr2-expressing progenitors located in the dorsal region of the SEZ. Examination of the progenitors of these glutamatergic interneurons allowed us to determine the sequential expression of transcription factors in these cells that are thought to be hallmarks of glutamatergic neurogenesis in the developing cerebral cortex and adult hippocampus. Indeed, the molecular specification of these SEZ progenitors allowed for their recruitment into the cerebral cortex after a lesion was induced. Taken together, our data indicate that SEZ progenitors not only produce a population of adult-born glutamatergic juxtaglomerular neurons, but may also provide a previously unknown source of progenitors for endogenous repair.
Brill
Monika S
MS
Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, Munich, Germany.
Ninkovic
Jovica
J
Winpenny
Eleanor
E
Hodge
Rebecca D
RD
Ozen
Ilknur
I
Yang
Roderick
R
Lepier
Alexandra
A
Gascón
Sergio
S
Erdelyi
Ferenc
F
Szabo
Gabor
G
Parras
Carlos
C
Guillemot
Francois
F
Frotscher
Michael
M
Berninger
Benedikt
B
Hevner
Robert F
RF
Raineteau
Olivier
O
Götz
Magdalena
M
D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology; D000367:Age Factors; D000818:Animals; D001369:Axons / Q000502:physiology; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001927:Brain Diseases / Q000473:pathology / Q000503:physiopathology; D019070:Cell Lineage / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology; D003712:Dendrites / Q000502:physiology; D004805:Ependyma / Q000166:cytology; D018698:Glutamic Acid / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D007395:Interneurons / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D018698:Glutamic Acid; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; D005680:gamma-Aminobutyric Acid; C491198:Eomes protein, mouse; C102938:Neurog2 protein, mouse