[Effects of PCB153 on oxidative stress and 8-OHdG content induced by PBDE-47 in human neuroblastoma cells in vitro].
OBJECTIVE
To explore the effects of PCB153 (2,2,4,4,5,5-hexachlorobiphenyl) on oxidative stress and 8-OHdG (8-hydroxy-2'-deoxyguanosine) content induced by PBDE-47 (2,2,4,4-tetrabromodiphenyl ether) in SH-SY5Y cells.


METHODS
SH-SY5Y cells were incubated with different concentrations of 1, 5, 10 micromol/L PBDE-47 or/and 5 micromol/L PCB153 and antioxidant N-acetyl cysteine (NAC 100 micromol/L) for 24h in vitro, ROS (reactive oxygen species) level and 8-OHdG were measured by using DCFH-DA fluorescent marking method and the skill of high performance liquid chromatogram-electrochemistry (HPLC-EC), respectively.


RESULTS
As for ROS formation, there was no significant difference in PBDE-47 groups alone compared to the control group and their corresponding combined groups, respectively (P > 0.05). ROS formation were significantly increased in 5 and 10 micromol/L combined groups compared to the control group and their corresponding concentrations of PBDE-47 groups or PCB153 group, respectively (P < 0.05). 8-OHdG levels were significantly increased in 10 micromol/L PBDE-47, 5 micromol/L PBDE-47 + 5 micromol/L PCB153, and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared with the control (P < 0.05). 8-OHdG level was dramatically increased in 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared to their corresponding doses of PBDE-47 groups or PCB153 group (P < 0.05). The groups coincubation with N-acetylcysteine caused a decrease in cellular ROS level and ameliorated PBDE-47-mediated oxidative DNA damage effects. Positive relationship was observed between ROS level and 8-OHdG contents (r = 0.895, P < 0.01).


CONCLUSIONS
These results provide evidence of a direct or indirect relationship between PBDE-47 and oxidative DNA damage. Furthermore, PBDE-47 combined with PCB153 may increase the effects on oxidative DNA damage in SH-SY5Y cells in vitro, oxidative stress may responsible for DNA damage induced by PBDE-47.
Gao
Ping
P
Department of Health Education, Medical College of CAPF, Tianjin 300162, China.
He
Ping
P
Wang
Aiguo
A
Xia
Tao
T
Xu
Zhixia
Z
Niu
Qiang
Q
Guo
Lijuan
L
Chen
Xuemin
X
D000080242:8-Hydroxy-2'-Deoxyguanosine; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
D055768:Halogenated Diphenyl Ethers; D017382:Reactive Oxygen Species; C511295:2,2',4,4'-tetrabromodiphenyl ether; D000080242:8-Hydroxy-2'-Deoxyguanosine; D011078:Polychlorinated Biphenyls; D003849:Deoxyguanosine; C014024:2,4,5,2',4',5'-hexachlorobiphenyl