title: [Chemical constituents from herbs of Paris verticillata].
abstract: OBJECTIVE
  To study the chemical constituents in herbs of Paris verticillata.
  
  
  METHODS
  The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 and RP-HPLC. The structures were identified by means of NMR analysis.
  
  
  RESULTS
  Nine compounds were isolated from the EtOAc extract and the n-BuOH extract of P. verticillata. Their structures were identified as beta-sitosterol (1), stigmasterol (2), daucosterol (3), beta-ecdysterone (4), 4-hydroxymethyl-gamma-butyrolactone (5), diosgenin-3-O-alpha-L-arabinofuranosyl (1-->4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (6), pennogenin-3-O-alpha-L-arabinofuranosyl(1-->4)-beta-D-glycopyranoside (7), pennogenin-3-O-alpha-L-arabinofuranosyl (1-->4)-[alpha-L-rhamnopy-ranosyl (1-->2)]-beta-D-glycopyranoside (8), and pennogenin-3-O-alpha-L-rhamno-pyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1--4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (9).
  
  
  CONCLUSIONS
  Compounds 1-9 are isolated from P. verticillata for the first time, and compounds 3, 5 are isolated from the genus Paris for the first time. The compounds 6-9 showed certain inhibition activeness of LA-795 cells, especially, the effects of compounds 6, 8 and 9 were more significant.
authors.affiliation: School of Pharmaceutical Science and Technolgy, Tianjin University, Tianjin 300072, China.
authors.forename: Xianxiao
authors.initials: X
authors.lastname: Huang
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Shuli
authors.initials: S
authors.lastname: Man
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yan
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice
chemical_list: D004365:Drugs, Chinese Herbal