Quantitative glycomics.
The ability to quantitatively determine changes is an essential component of comparative glycomics. Multiple strategies are available by which this can be accomplished. These include label-free approaches and strategies where an isotopic label is incorporated into the glycans prior to analysis. The focus of this chapter is to describe each of these approaches while providing insight into their strengths and weaknesses, so that glycomic investigators can make an educated choice of the strategy that is best suited for their particular application.
Orlando
Ron
R
Departments of Biochemistry & Molecular Biology, and Chemistry, Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007554:Isotopes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D011134:Polysaccharides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling / Q000379:methods
D007554:Isotopes; D011134:Polysaccharides