[Protective effects of peptides from velvet antler of Cervus nippon on acute ischemic myocardial injury in rats].
OBJECTIVE
To investigate the cardioprotective effects of the peptides from the velvet antler (VAP) of Cervus nippon on acute ischemic myocardial injury in rats and its underlying mechanisms.


METHODS
The model rats of myocardial ischemia injury (MI) was produced by ligating the left anterior descending (LAD) branch of the coronary artery of rats. The rats were divided randomly into six groups: sham-operated group; ischemic myocardial injury (MI) model group; diltiazem hydrochloride 1 mg x kg(-1) group; VAP 30 mg x kg(-1) group; VAP 60 mg x kg(-1) group; VAP 120 mg x kg(-1) group. VAP or diltiazem hydrochloride (calcium-antagonist) was injected into the tail vein of each of the experimental rats 12 h and 23 h respectively after left anterior descending (LAD) occlusion. The same volume of saline was administered into the sham-operated group and MI model group. The ischemic size, ST elevation, serum levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST), superoxide dismutase (SOD), malondialdehyde (MDA), and levels of SOD, MDA in myocardial tissue were measured in the rats at 24 h after LAD occlusion.


RESULTS
The ischemic risk area ratio was 34.52 +/- 8.69% in the MI control group. Treatment of VAP at doses of 60 and 120 mg x kg(-1) resulted in reductions in the infarct size of (21.67 +/- 5.19)% and (19.56 +/- 9.15)% (P < 0.05), respectively. Treatment with VAP at doses of 30, 60 and 120 mg x kg(-1) significantly decreased ST elevation (0.28 +/- 0.03, 0.21 +/- 0.03, 0.16 +/- 0.02, 0.15 +/- 0.02) mV (all P < 0.01), compared with the MI control rats (0.41 +/- 0.05) mV. VAP also significantly decreased levels of CK, LDH, AST, MDA in serum and myocardial tissue, increase SOD activity in serum and myocardial tissue in MI rats.


CONCLUSIONS
VAP exerts significant cardioprotective effects against acute ischemic myocardial injury in rats, likely through its antioxidant and anti-lipid peroxidation properties.
Chen
Xiaoguang
X
College of Life Science, Jilin University, Changchun 130021, China. xg_chen@163.com
Wang
Yan
Y
Wu
Yan
Y
Wang
Liping
L
Li
Wei
W
D000208:Acute Disease / Q000628:therapy; D000818:Animals; D000999:Antlers / Q000737:chemistry* / Q000378:metabolism; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D010455:Peptides / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012418:Ruminants / Q000378:metabolism
D002316:Cardiotonic Agents; D010455:Peptides