title: Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.
abstract: A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.
authors.affiliation: Russian State Medical University, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Usovetskii
authors.forename: V V
authors.initials: VV
authors.lastname: Burunova
authors.forename: N E
authors.initials: NE
authors.lastname: Kovtun
authors.forename: Yu G
authors.initials: YG
authors.lastname: Suzdal'tseva
authors.forename: Yu B
authors.initials: YB
authors.lastname: Krasil'nikova
authors.forename: N G
authors.initials: NG
authors.lastname: Korotkii
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D001706:Biopsy; D018920:Coculture Techniques; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008544:Melanocytes / Q000166:cytology*; D012880:Skin Pigmentation; D014820:Vitiligo / Q000473:pathology*