The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids.
The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 and 260 nm. Principal component analysis of the spectra allowed their differentiation into a number of groups related to the means of their production reflecting the structural changes introduced by each process. The findings suggest that CD provides a complementary technique for the rapid analysis of heparin preparations.
Rudd
Timothy R
TR
The University of Liverpool, School of Biological Sciences, Liverpool, Merseyside L69 7ZB, UK.
Skidmore
Mark A
MA
Guimond
Scott E
SE
Holman
Joseph
J
Turnbull
Jeremy E
JE
Lauder
Robert M
RM
Fernig
David G
DG
Yates
Edwin A
EA
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D002942:Circular Dichroism; D016903:Drug Monitoring / Q000379:methods*; D005163:Factor Analysis, Statistical; D006025:Glycosaminoglycans / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry* / Q000302:isolation & purification; D006496:Heparinoids / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D015394:Molecular Structure; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013552:Swine
D000925:Anticoagulants; D006025:Glycosaminoglycans; D006495:Heparin, Low-Molecular-Weight; D006496:Heparinoids