STUDIES ON ENZYMATIC HISTOCHEMISTRY : XXV. A MICRO METHOD FOR THE DETERMINATION OF CHOLINE ESTERASE AND THE ACTIVITY-PH RELATIONSHIP OF THIS ENZYME.
The activity-pH relationship for choline esterase from horse serum, gastric mucosa of the pig, and cat brain was investigated, and an optimum was observed at pH 8.5 in each case. A micro method for the determination of choline esterase was developed capable of measuring the hydrolysis down to the order of that given by 1 x 10(-8) mol of ester.
Glick
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Carlsberg Laboratory, Copenhagen.