Expanding the repertoire of glycosynthases.
As reported in this issue, Moracci and coworkers have now expanded the glycosynthase concept to two retaining alpha-L-fucosidases. The newly generated alpha-fucosynthases can use beta-L-fucosyl azide as donors for transglycosylation, enabling the synthesis of fucose-containing oligosaccharides.
Wang
Lai-Xi
LX
Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. lwang@som.umaryland.edu
D019943:Amino Acid Substitution; D055162:Biocatalysis; D006031:Glycosylation; D016297:Mutagenesis, Site-Directed; D009844:Oligosaccharides / Q000096:biosynthesis; D005644:alpha-L-Fucosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D009844:Oligosaccharides; D005644:alpha-L-Fucosidase