Vasorelaxant and antihypertensive effects of methanolic extract from roots of Laelia anceps are mediated by calcium-channel antagonism.
RMELanc-induced relaxation in aortic rings precontracted with NE, 5-HT and KCl. It also reduced NE-induced transient contraction in Ca(2+)-free solution and inhibited contraction induced by increasing external calcium. Nevertheless, the vasorelaxant effect of RMELanc was not reduced by ODQ, 1-alprenolol, TEA, glibenclamide, and 2-AP. Oral administration of 100 mg/kg of RMELanc exhibited a significant decrease in systolic and diastolic blood pressures in SHR rats. HPLC analysis allowed us to detect the presence of 2,7-dihydroxy-3,4,9-trimethoxyphenantrene (1), which induced a significant relaxation effect. Therefore, our results suggest that RMELanc induces vasorelaxant and antihypertensive effects by blockade of Ca(2+) channels.
Vergara-Galicia
Jorge
J
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
Ortiz-Andrade
Rolffy
R
Rivera-Leyva
Julio
J
Castillo-España
Patricia
P
Villalobos-Molina
Rafael
R
Ibarra-Barajas
Maximiliano
M
Gallardo-Ortiz
Itzell
I
Estrada-Soto
Samuel
S
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001011:Aorta; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D029595:Orchidaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000032:analysis / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000494:pharmacology*
C554119:2,7-dihydroxy-3,4,9-trimethoxyphenantrene; D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D010616:Phenanthrenes; D014665:Vasodilator Agents; D002118:Calcium