Optimization of medium and cultivation conditions for L-amino acid oxidase production by Aspergillus fumigatus.
A fungal strain was selected from the microbial repository of the North-East Institute of Science and Technology, Jorhat, India, which could produce a high yield of L-amino acid oxidase. 18SrRNA, ITS1, 5.8SrRNA ITS2, and partial 28 S rRNA sequencing and phenotypic characteristics indicate that it belong to the species Aspergillus fumigatus (designated as P13). Maximum production of enzyme (59.55 x 10-3 U/mg dry cell mass) was obtained in a medium containing 10 g/L glucose, 4 g/L yeast extract, and 4 g/L ammonium sulfate, with 20 mmol/L of L-threonine as the inducer. The optimum temperature for enzyme production was 30 degrees C at pH 7.0, with a shaking speed of 200 r/min. At 96 h, the enzyme activity was maximum. The A. fumigatus P13 L-amino acid oxidase accepts a broad substrate range, and the maximum enzyme activity (20.41 x 10-3 U/mg dry cell mass) was obtained with 50 mmol/L of L-tyrosine. In the literature, no reports have been found regarding the production of L-amino acid oxidase by A. fumigatus. The enzyme showed enantiomerically pure amino acid formation, which has tremendous demand in industrial applications.
Singh
Susmita
S
North-East Institute of Science and Technology, Council of Scientific and Industrial Research, Jorhat, Assam, India. susmitasingh123@gmail.com
Gogoi
B K
BK
Bezbaruah
R L
RL
D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis*; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007194:India; D007700:Kinetics; D050830:L-Amino Acid Oxidase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; D050830:L-Amino Acid Oxidase