PEI-alginate nanocomposites: efficient non-viral vectors for nucleic acids.
Branched polyethylenimine (PEI, 25 kDa) was ionically interacted with varying amount of alginic acid to block different proportion (2.6-5.7%) of amines in PEI to form a series of nanocomposites, PEI-Al. These nanocomposites, upon interaction with DNA, protected it against DNase I. Among various complexes evaluated, PEI-Al(4.8%)/DNA displayed the highest transfection efficiency in HEK293, COS-1 and HeLa cells that was approximately 2-8-folds higher than Superfect, Fugene, PEI (750 kDa)-Al(6.26%) and PEI alone. The projected nanocomposites were nearly non-toxic to cells in vitro. Furthermore, the concentration of PEI-Al(4.8%) needed to deliver GFP-specific siRNA in COS-1 cells was 20 times lower than PEI (750 kDa)-Al(6.26%). Intracellular trafficking of PEI-Al(4.8%) with or without complexed DNA in HeLa cells shows that both appear in the nucleus after 1 h.
Patnaik
Soma
S
Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Mall Road, Delhi 110007, India.
Arif
Mohammed
M
Pathak
Atul
A
Singh
Naresh
N
Gupta
K C
KC
D021581:Active Transport, Cell Nucleus; D000464:Alginates / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006367:HeLa Cells; D006603:Hexuronic Acids / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D053758:Nanoparticles; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D000464:Alginates; D006603:Hexuronic Acids; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D004247:DNA; D003850:Deoxyribonuclease I