[Case-control, family based and screening for DNA sequence variation in the dopamine transporter gene polymorphism DAT 1 in alcohol dependence].
OBJECTIVE
The paper focuses on candidate gene polymorphism and on the role of dopamine transporter gene polymorphism DAT 1 in the pathogenesis of alcoholism. We investigated this polymorphism in the association study in a whole group of alcoholics (n = 150), fathers (n = 84) and mothers (n = 101) and patients with alcohol dependence (n = 103). The control group consisted of healthy volunteers with excluded psychiatric disorders, gender and age matched (n = 183). The transmission disequilibrium test (TDT) was used in the study. At the last stage of the study we screened the DNA sequence and compared 9 VNTR and 10 VNTR. METHOD; The history of alcoholism was obtained using the SSAGA (Semi-Structured Assessment for the Genetics of Alcoholism - Polish version). The DAT 1 polymorphism was determined using PCR. Screening for DNA sequence variation in the dopamine transporter gene polymorphism DAT 1 was determined using ABI 310.


RESULTS
We did not find significant differences in the case-controlled study. The alleles and genotypes distribution of the investigated polymorphism did not differ significantly between the alcoholics and the controls in the case-control study. We found significant differences in allele transmission in our patient group (n = 77) 10 VNTR 63% and 9 VNTR 37% (p = 0.033), from mothers to proband (p = 0.049) and a statistical trend to frequent 10 VNTR allele transmission from the fathers (p = 0.071). Screening for DNA sequence variation in the dopamine transporter gene polymorphism DAT 1 showed the number 9 repeat in 9 VNTR as missing.


CONCLUSIONS
The results of this study suggest that DAT 1 gene polymorphism plays a role in alcohol dependence.
Grzywacz
Anna
A
Katedra i Klinika Psychiatrii Pomorskiej AM w Szczecinie.
Samochowiec
Jerzy
J
D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000235:genetics*; D000437:Alcoholism / Q000145:classification* / Q000175:diagnosis / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D055815:Young Adult
D050483:Dopamine Plasma Membrane Transport Proteins