Effect of recombinant heat shock protein 70 of mycobacterial origin on cytotoxic activity and immunophenotype of human peripheral blood mononuclear leukocytes.
Recombinant heat shock protein of mycobacterial origin with a molecular weight 70 kDa in concentration 0.1 microg/ml in vitro activates cytotoxic activity of mononuclear lymphocytes from peripheral blood of healthy donors against K-562 human erythroblastic leukemia cells sensitive to natural killers. In other concentrations (1.0 microg/ml and 10 microg/ml) this heat shock protein did not significantly affect functional activity of mononuclear leukocytes. Expression of CD4, CD25, CD16 and CD56 on membrane of mononuclear leukocytes was also studied.
Vorobiev
D S
DS
I. I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia. vorobievdenis@yandex.ru
Kiselevskii
M V
MV
Chikileva
I O
IO
Semenova
I B
IB
D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000494:pharmacology
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins