Arthrobacter alpinus sp. nov., a psychrophilic bacterium isolated from alpine soil.
An aerobic, Gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain S6-3(T), was isolated from alpine soil. Cells exhibited a rod-coccus growth cycle and produced a yellow pigment. Growth occurred at 1-25 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S6-3(T) was related to members of the genus Arthrobacter, sharing highest sequence similarities with the type strains of Arthrobacter psychrolactophilus (97.9 %) and Arthrobacter stackebrandtii (97.6 %). Strain S6-3(T) had MK-9(H(2)) as the major menaquinone and anteiso-C(15 : 0) as the major fatty acid. The cell-wall peptidoglycan was of type A3alpha l-Lys-l-Thr-Ala(3). The predominant cell-wall sugars were galactose and rhamnose. The genomic DNA G+C content of strain S6-3(T) was 61.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain S6-3(T) is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter alpinus sp. nov. is proposed. The type strain is S6-3(T) (=DSM 22274(T) =CGMCC 1.8950(T)).
Zhang
De-Chao
DC
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Schumann
Peter
P
DSMZ - Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Liu
Hong-Can
HC
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Xin
Yu-Hua
YH
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhou
Yu-Guang
YG
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Schinner
Franz
F
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Margesin
Rosa
R
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
D001173:Arthrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S