Inhibition of NF-kappaB expression and allergen-induced airway inflammation in a mouse allergic asthma model by andrographolide.
Andrographolide from traditional Chinese herbal medicines previously showed it possesses a strong anti-inflammatory activity. In present study, we investigated whether Andrographolide could inhibit allergen-induced airway inflammation and airways hyper-responsiveness and explored the mechanism of Andrographolide on allergen-induced airway inflammation and airways hyper-responsiveness. After sensitized and challenged by ovalbumin, the BALB/c mice were administered intraperitoneally with Andrographolide. Hyper-responsiveness was recorded. The lung tissues were assessed by histological examinations. NF-kappaB in lung was determined by immunofluorescence staining and Western blotting. Treatment of mice with Androqrapholide displayed lower Penh in response to asthma group mice. After treatment with Andrographolide, the extent of inflammation and cellular infiltration in the airway were reduced. Andrographolide interrupted NF-kappaB to express in cell nucleus. The level of NF-kappaB expression was inhibited by Andrographolide. The data indicate that Andrographolide from traditional Chinese herbal medicines could inhibit extensive infiltration of inflammatory cells in lung and decrease airway hyperreactivity. Andrographolide could inhibit NF-kappaB expression in lung and suppress NF-kappaB expressed in the nucleus of airway epithelial cells.
Li
Jing
J
Inflammation & Allergic Disease Lab, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China.
Luo
Li
L
Wang
Xiaoyun
X
Liao
Bin
B
Li
Guoping
G
D000485:Allergens; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000188:drug therapy*; D055745:Bronchioles / Q000187:drug effects / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology
D000485:Allergens; D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; C036457:andrographoside; D010047:Ovalbumin