Humoral and cell-mediated immune-responses after administration of a single-shot recombinant hepatitis B surface antigen vaccine formulated with cationic poly(l-lactide) microspheres.
The present investigations were aimed to compare the humoral and cell-mediated immune responses between recombinant hepatitis B surface antigens (HBsAg) adsorbed L-PLA microspheres (Ms) vaccine (single-shot) and marketed alum-HBsAg vaccine (two-doses). The blank cationic (cetyltrimethyammoniumbromide) microspheres were prepared by the double emulsion (w/o/w) solvent evaporation technique. The HBsAg was adsorbed onto the surface of blank cationic microspheres. These microspheres were characterized in vitro for their size, shape, adsorption-efficiency, in-process stability, and HBsAg release studies. Specific humoral immune responses (IgM and IgG) and cell-mediated immune responses (cellular-proliferation) assay including release of interferon-gamma (IFN-gamma), interleukin-2 (IL-2), and nitric oxide (NO) from host's cells stimulated with HBsAg or lipopolysaccharide (LPS)/ concanavalin A (con A) in-vitro were determined. Based on these findings, it was concluded that the single injection (using subcutaneous-route) of the polymeric microspheres produced better immune response (both humoral and cell-mediated) than two injections of a conventional alum-HBsAg vaccine. These data demonstrate high potential of polymeric microspheres for their use as a carrier adjuvant for hepatitis B vaccine.
Saini
Vinay
V
Biopharmaceuticals Research Laboratory, Deparment of Pharmaceutical Sciences, Dr. Harisingh Gour University, Madhya Pradesh, India.
Jain
Vikas
V
Sudheesh
M S
MS
Dixit
Saurabh
S
Gaur
R L
RL
Sahoo
M K
MK
Joseph
S K
SK
Verma
S K
SK
Jaganathan
K S
KS
Murthy
P K
PK
Kohli
Dharmveer
D
D000818:Animals; D002412:Cations; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*
D002412:Cations; D004337:Drug Carriers; D004655:Emulsions; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011091:Polyesters; C033616:poly(lactide)