Isolation and promoter analysis of anther-specific genes encoding putative arabinogalactan proteins in Malus x domestica.
In this study, we searched for anther-specific genes involved in male gametophyte development in apple (Malus x domestica Borkh. cv. Fuji) by differential display-PCR. Three full-length cDNAs were isolated, and the corresponding genomic sequences were determined by genome walking. The identified genes showed intronless 228- to 264-bp open reading frames and shared 82-90% nucleotide sequence. Sequence analysis identified that they encoded a putative arabinogalactan protein (AGP) and were designated MdAGP1, MdAGP2, and MdAGP3, respectively. RT (reverse transcriptase)-PCR revealed that the MdAGP genes were selectively expressed in the stamen. Promoter analysis confirmed that the MdAGP3 promoter was capable of directing anther- or pollen-specific expression of the GUS reporter in tobacco and apple. Furthermore, expression of ribosome-inactivating protein under the control of the MdAGP3 promoter induced complete sporophytic male sterility as we had expected.
Choi
Yeon-Ok
YO
Biotech Research Team, Dongbu Advanced Research Institute, Dongbu HiTek Co., Ltd, Daejeon, Korea.
Kim
Sung-Soo
SS
Lee
Sanghyeob
S
Kim
Sunggil
S
Yoon
Gi-Bo
GB
Kim
Hyojeong
H
Lee
Young-Pyo
YP
Yu
Gyung-Hee
GH
Hyung
Nam-In
NI
Sung
Soon-Kee
SK
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D018745:Genome, Plant; D027845:Malus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism; D051479:Plant Infertility; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics
D018076:DNA, Complementary; D018744:DNA, Plant; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins