Tailoring the soliton output of a photonic crystal fiber for enhanced two-photon excited luminescence response from fluorescent protein biomarkers and neuron activity reporters.
Dual-cladding photonic crystal fibers (PCFs) with two zero-dispersion points are used to enhance the two-photon excited luminescence (TPL) response from fluorescent protein biomarkers and neuron activity reporters in dye-cell experiments and in in vivo work on transgenic mice and tadpoles. The soliton transmission of ultrashort pulses through a PCF suppresses dispersion-induced temporal pulse spreading, maintaining a high level of field intensity needed for efficient TPL excitation. The soliton self-frequency shift, stabilized against laser power fluctuations by a specific PCF dispersion design, is employed to accurately match the wavelength of the soliton PCF output with the two-photon absorption spectrum of dye or fluorescent protein biomarker molecules, enhancing their TPL response and allowing the laser damage of biotissues to be avoided.
Doronina
Lyubov V
LV
Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Russia.
Fedotov
Il'ya V
IV
Voronin
Aleksandr A
AA
Ivashkina
Olga I
OI
Zots
Marina A
MA
Anokhin
Konstantin V
KV
Rostova
Ekaterina
E
Fedotov
Andrei B
AB
Zheltikov
Aleksei M
AM
D000042:Absorption; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D007834:Lasers; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D017785:Photons; D013050:Spectrometry, Fluorescence
D015415:Biomarkers; D008164:Luminescent Proteins