title: Markers of haemostasis and angiogenesis in placentae from gestational vascular complications: impairment of mechanisms involved in maintaining intervillous blood flow.
abstract: BACKGROUND
  Preeclampsia (PE) and fetal growth restriction (FGR) are multifactorial diseases, whose pathogenesis is largely unknown. A significant relationship between haemostasis and angiogenesis in placentae from uneventful pregnancies was previously shown.
  
  
  METHODS
  RNA expression of haemostasis (TF, TFPI, TFPI-2, PAI-2, Anx V, TM) and angiogenesis (Ang-1, Ang-2, PlGF, VEGF) markers in placentae from PE (n=12), PE+FGR (n=17) and FGR (n=20) in respect of placentae from uncomplicated pregnancies (n=21) were investigated.
  
  
  RESULTS
  Placentae from complicated pregnancies showed a significant lower expression (p<or=0.05 Mann-Whitney U test) of TF, TFPI, TFPI-2, Anx V, PAI-2 than those from in uncomplicated ones. VEGF and PlGF were not different in the considered groups; Ang-1 and Ang-2 were significantly higher (p<or=0.05 Mann-Whitney U test) in the PE group. Correlations between factors involved in haemostasis and those involved in angiogenesis, observed in placentae from uneventful pregnancies are lacking in those from complicated ones.
  
  
  CONCLUSIONS
  Haemostasis factors are reduced in placentae from complicated pregnancies. The relationship between haemostasis and angiogenesis observed in uncomplicated pregnancies is impaired in PE and FGR.
authors.affiliation: Atherosclerosis and Thrombosis Unit, I.R.C.C.S. Casa Sollievo della Sofferenza, S.Giovanni Rotondo, Foggia, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Chinni
authors.forename: Donatella
authors.initials: D
authors.lastname: Colaizzo
authors.forename: Giovanni L
authors.initials: GL
authors.lastname: Tiscia
authors.forename: Pasquale
authors.initials: P
authors.lastname: Martinelli
authors.forename: Giuseppe M
authors.initials: GM
authors.lastname: Maruotti
authors.forename: Maria
authors.initials: M
authors.lastname: Matteo
authors.forename: Maurizio
authors.initials: M
authors.lastname: Margaglione
authors.forename: Elvira
authors.initials: E
authors.lastname: Grandone
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000098:blood supply; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010920:Placenta / Q000098:blood supply* / Q000166:cytology / Q000502:physiology*; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000378:metabolism*; D012039:Regional Blood Flow
chemical_list: D015415:Biomarkers