Surface modification with BSA blocking based on in situ synthesized gold nanoparticles in poly(dimethylsiloxane) microchip.
A stable BSA blocking poly(dimethylsiloxane) (PDMS) microchannel was prepared based on in situ synthesized PDMS-gold nanoparticles composite films. The modified microchip could successfully suppress protein adsorption. The assembly was followed by contact angle, charge-coupled device (CCD) imaging, electroosmotic flow (EOF) measurements and electrophoretic separation methods. Contact angle measurements revealed the coated surface was hydrophilic, water contact angle for coated chips was 45.2 degrees compared to a water contact angle for native PDMS chips of 88.5 degrees. The coated microchips exhibited reproducible and stable EOF behavior. With FITC-labeled myoglobin incubation in the coated channel, no fluorescence was observed with CCD image, and the protein exhibited good electrophoretic effect in the modified microchip.
Fan
Da-He
DH
School of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng 224003, PR China.
Yuan
Shi-Wei
SW
Shen
Yong-Miao
YM
D000327:Adsorption; D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000378:metabolism*; D053841:Electroosmosis; D016650:Fluorescein-5-isothiocyanate; D005453:Fluorescence; D006046:Gold / Q000378:metabolism*; D006736:Horses; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D009211:Myoglobin / Q000378:metabolism; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D009211:Myoglobin; D012710:Serum Albumin, Bovine; D006046:Gold; D016650:Fluorescein-5-isothiocyanate