title: Benthic bacterial diversity in submerged sinkhole ecosystems.
abstract: Physicochemical characterization, automated ribosomal intergenic spacer analysis (ARISA) community profiling, and 16S rRNA gene sequencing approaches were used to study bacterial communities inhabiting submerged Lake Huron sinkholes inundated with hypoxic, sulfate-rich groundwater. Photosynthetic cyanobacterial mats on the sediment surface were dominated by Phormidium autumnale, while deeper, organically rich sediments contained diverse and active bacterial communities.
authors.affiliation: University of Wisconsin-Stout, 410 10th Avenue East, Menomonie, WI 54751, USA. nolds@uwstout.edu
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Nold
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Pangborn
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Zajack
authors.forename: Scott T
authors.initials: ST
authors.lastname: Kendall
authors.forename: Richard R
authors.initials: RR
authors.lastname: Rediske
authors.forename: Bopaiah A
authors.initials: BA
authors.lastname: Biddanda
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S