title: Reduced circulating antioxidant defences are associated with airway hyper-responsiveness, poor control and severe disease pattern in asthma.
abstract: Dietary antioxidants are important in protecting against oxidative stress. We have previously demonstrated that circulating dietary antioxidant levels are reduced in asthma. The present study examined the variation in dietary antioxidant levels in asthma, according to airway responsiveness, asthma control and clinical asthma pattern. Peripheral blood was collected from forty-one subjects with stable, persistent asthma. Airway responsiveness was assessed by hypertonic saline challenge. Asthma control was assessed using the Asthma Control Questionnaire. Clinical asthma pattern was determined using Global Initiative for Asthma (GINA) criteria. Whole-blood carotenoids (beta-carotene, lycopene, alpha-carotene, beta-cryptoxanthin, lutein/zeaxanthin) and tocopherols (alpha-, delta-, gamma-tocopherol) were measured by HPLC. Plasma antioxidant potential (AOP) was determined by colorimetric assay (OxisResearch, Portland, OR, USA). Asthmatic subjects with airway hyper-responsiveness (AHR) had reduced levels of beta-carotene and alpha-tocopherol compared with those without AHR. Subjects with uncontrolled asthma had low levels of AOP compared with those with controlled or partly controlled asthma. Subjects with a severe persistent clinical asthma pattern had reduced levels of alpha-tocopherol compared with those with a mild to moderate asthma pattern. We conclude that asthmatic subjects with AHR, uncontrolled asthma and a severe asthma pattern have impaired antioxidant defences and are thus most susceptible to the damaging effects of oxidative stress. This highlights the potential role for antioxidant supplementation in these subjects.
authors.affiliation: Respiratory and Sleep Medicine, Hunter Medical Research Institute, John Hunter Hospital, Newcastle, NSW, Australia. lisa.wood@newcastle.edu.au
authors.forename: Lisa G
authors.initials: LG
authors.lastname: Wood
authors.forename: Peter G
authors.initials: PG
authors.lastname: Gibson
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism*; D001249:Asthma / Q000097:blood* / Q000503:physiopathology / Q000628:therapy; D016535:Bronchial Hyperreactivity / Q000097:blood*; D002338:Carotenoids / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012720:Severity of Illness Index; D011795:Surveys and Questionnaires; D024505:Tocopherols / Q000008:administration & dosage / Q000097:blood*; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000097:blood; D019207:beta Carotene / Q000008:administration & dosage / Q000097:blood
chemical_list: D000975:Antioxidants; D019207:beta Carotene; D002338:Carotenoids; D024502:alpha-Tocopherol; D024505:Tocopherols