Neuronal degeneration and microglial activation in the ischemic dentate gyrus of the gerbil.
In the present study, we investigated the time-course changes of neuronal degeneration and microglial activation in the gerbil dentate gyrus after transient cerebral ischemia using Fluoro-Jade B histofluorescence staining and immunohistochemistry for Iba-1. Fluoro-Jade B positive cells were observed from 6 hr and markedly increased 1 day after ischemia/reperfusion. Iba-1-immunoreactive microglia were increased and hypertrophied at early time, and Iba-1 immunoreactivity was highest at 2 days after ischemia/reperfusion. These results may be direct evidence on neuronal degeneration and microglial activation in the gerbil dentate gyrus after ischemia/reperfusion.
Moon
Joong Bum
JB
Department of Emergency Medicine, College of Medicine, Kangwon National University, Chuncheon 200-702, South Korea.
Lee
Choong Hyun
CH
Park
Chan Woo
CW
Cho
Jun Hwi
JH
Hwang
In Koo
IK
Yoo
Ki-Yeon
KY
Choi
Jung Hoon
JH
Shin
Hyung-Cheul
HC
Won
Moo-Ho
MH
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology*; D018891:Dentate Gyrus / Q000098:blood supply / Q000473:pathology*; D005849:Gerbillinae; D017628:Microglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D015427:Reperfusion Injury / Q000473:pathology*