title: Nanoscale in situ morphological study of proteins immobilized on gold thin films.
abstract: The nanoscale organization of acetylcholinesterase (AChE) and of its polyclonal antibody immobilized on gold thin films was studied by means of Energy Dispersive X-ray Reflectometry (EDXR) and Atomic Force Microscopy (AFM). The macromolecules were alternatively deposited over a self-assembled monolayer (SAM) of N-hydroxysuccinimide esters of thioctic acid. The measurements, collected in situ at subsequent deposition stages of the device, gave information on the distribution of the macromolecules on the surface showing that both the proteins can bind covalently to the SAM. In addition to this, we demonstrated that the antigen-antibody reaction takes place when one of the two reactants is anchored to the surface.
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale A.Moro, 5 - Roma 00185, Italy.
authors.forename: Francesca A
authors.initials: FA
authors.lastname: Scaramuzzo
authors.forename: Romina
authors.initials: R
authors.lastname: Salvati
authors.forename: Barbara
authors.initials: B
authors.lastname: Paci
authors.forename: Amanda
authors.initials: A
authors.lastname: Generosi
authors.forename: Valerio
authors.initials: V
authors.lastname: Rossi-Albertini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Latini
authors.forename: Mario
authors.initials: M
authors.lastname: Barteri
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D006046:Gold; D000110:Acetylcholinesterase