title: Plasma interleukin-1beta concentration is associated with stroke in sickle cell disease.
abstract: The pathogenesis of sickle cell disease (HbSS), which has numerous complications including stroke, involves inflammation resulting in alteration of plasma inflammatory protein concentration. We investigated HbSS children with abnormal cerebral blood flow detected by trans-cranial Doppler ultrasound (TCD) who participated in the multi-center stroke prevention (STOP) study, to determine if plasma inflammatory protein concentration is associated with the outcome of stroke. Thirty-nine plasma samples from HbSS participants with elevated TCD who had no stroke, HbSS-NS (n=13) or had stroke, HbSS-S (n=13), HbSS steady-state controls (n=7) and controls with normal hemoglobin, HbAA (n=6), were analyzed simultaneously for 27 circulating inflammatory proteins. Logistic regression and receiver operating characteristics curve analysis of stroke on plasma inflammatory mediator concentration, adjusted for age and gender, demonstrated that interleukin-1beta (IL-1beta) was protective against stroke development (HbSS-NS=19, 17-23, HbSS-S=17, 16-19 pg/mL, median and 25th-75th percentile; odds ratio=0.59, C.I.=0.36-0.96) and was a good predictor of stroke (area under curve=0.852). This result demonstrates a strong association of systemic inflammation with stroke development in HbSS via moderately increased plasma IL-1beta concentration, which is furthermore associated with a decreased likelihood of stroke in HbSS.
authors.affiliation: Morehouse School of Medicine, Atlanta, GA 30310, USA.
authors.forename: Kwaku
authors.initials: K
authors.lastname: Asare
authors.forename: Beatrice E
authors.initials: BE
authors.lastname: Gee
authors.forename: Jonathan K
authors.initials: JK
authors.lastname: Stiles
authors.forename: Nana O
authors.initials: NO
authors.lastname: Wilson
authors.forename: Adel
authors.initials: A
authors.lastname: Driss
authors.forename: Alexander
authors.initials: A
authors.lastname: Quarshie
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Adams
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kutlar
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Hibbert
mesh_terms: D000293:Adolescent; D000755:Anemia, Sickle Cell / Q000097:blood / Q000150:complications / Q000276:immunology; D001783:Blood Flow Velocity; D002560:Cerebrovascular Circulation; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood* / Q000276:immunology; D016015:Logistic Models; D008297:Male; D012372:ROC Curve; D012039:Regional Blood Flow; D020521:Stroke / Q000097:blood / Q000209:etiology / Q000276:immunology; D017585:Ultrasonography, Doppler, Transcranial; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta