A new ent-kaurane type diterpenoid glycoside from Inula japonica Thunb.
A new ent-kaurane type diterpenoid glycoside, 17-O-beta-D-glucopyranosyl-16alpha-ent-kauran-19-oic acid (1), together with 17-hydroxy-16alpha-ent-kauran-19-oic acid (2), 16alpha,17-dihydroxyl-ent-kauran-19-oic acid (3), and 16alpha-hydroxy-17-acetoxy-ent-kauran-19-oic acid (4) were isolated from the aerial parts of Inula japonica Thunb. The structure of 1 was determined mainly by use of 1D and 2D NMR spectroscopic techniques including HSQC, (1)H-(1)H COSY, HMBC, and NOESY. In addition, 4 exhibited significant inhibitory activity on NO production in LPS-stimulated RAW264.7 cells with IC(50) value of 14.3 microg/mL.
Qin
Jiang Jiang
JJ
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Zhu
Jia Xian
JX
Zhang
Wei Dong
WD
Zhu
Yan
Y
Fu
Jian Jun
JJ
Liu
Xiao Hua
XH
Jin
Hui Zi
HZ
D000818:Animals; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D045786:Diterpenes, Kaurane / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D038143:Inula / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D035261:Plant Components, Aerial / Q000737:chemistry
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D009569:Nitric Oxide