Determination of beta-hydroxybutyrate in blood and urine using gas chromatography- mass spectrometry.
Beta-hydroxybutyrate (BHB) is considered a potential biomarker for alcoholic ketoacidosis (AKA). A robust and sensitive method was developed and validated for the quantitative determination of BHB in postmortem blood and urine using deuterated gamma-hydroxybutyrate as an internal standard. Samples were analyzed by gas chromatography-mass spectrometry following liquid-liquid extraction and silyl derivatization. The limits of detection and lower limits of quantification in blood and urine were 2 and 7 mg/L and 2 and 6 mg/L, respectively. The interday and intraday precision was measured by coefficients of variation for blood and urine and ranged from 1.0 to 12.4% for quality control samples spiked at 50 and 300 mg/L. The linear range of 50-500 mg/L resulted in an average correlation of R(2) > 0.99, and the average extraction recoveries in blood and urine were >or= 82% and >or= 59%, respectively. BHB remains stable in blood spiked at a concentration of 300 mg/L for 15 days when stored within a refrigerator (2-5 degrees C). Postmortem blood and urine samples were analyzed using the validated method for cases where the deceased had a history of chronic alcohol abuse to establish the use of BHB as a potential marker of AKA.
Hassan
Huda M A
HM
Forensic Medicine and Science, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom. Huda.Hassan@formed.gla.ac.uk
Cooper
Gail A A
GA
D020155:3-Hydroxybutyric Acid / Q000032:analysis* / Q000737:chemistry; D000437:Alcoholism / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000737:chemistry; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007662:Ketosis / Q000175:diagnosis / Q000378:metabolism; D008297:Male; D011237:Predictive Value of Tests; D015203:Reproducibility of Results
D015415:Biomarkers; D020155:3-Hydroxybutyric Acid