A functional polymorphism in Pre-miR-146a gene is associated with prostate cancer risk and mature miR-146a expression in vivo.
BACKGROUND
A G > C polymorphism (rs2910164) which is located in the sequence of miR-146a precursor, results in a change from a G:U pair to a C:U mismatch in its stem region. To explore whether rs2910164 plays any role in prostate cancer (CaP), we analyzed the association between miR-146a polymorphism and risk of CaP and the expression of miR-146a with different genotypes in CaP tissues in southern Chinese Han population.


METHODS
Two hundred fifty-one CaP and 280 control subjects were included in the cancer association study, and 15 CaP tissue samples were used to test the expression of the miRNA precursors by real-time quantitative reverse transcription PCR.


RESULTS
We found that subjects carrying CC homozygotes had a 0.65-fold reduced risk (95% CI = 0.43-0.99) than those carrying GG/GC genotypes (P = 0.03), and the C allele displayed a lower prevalence of CaP compared with the G allele (OR = 0.73, 95% CI = 0.57-0.94, P = 0.01). Moreover, hsa-miR-146a quantification showed that homozygous carriers of the C-variant had significantly decreased miRNA levels compared to the carriers of the GG/GC genotype.


CONCLUSIONS
The natural genetic variation in pre-miR-146a affects the amount of mature miR-146a, contributes to the genetic predisposition to CaP.
Xu
Bin
B
Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Feng
Ning-Han
NH
Li
Peng-Chao
PC
Tao
Jun
J
Wu
Deyao
D
Zhang
Zheng-Dong
ZD
Tong
Na
N
Wang
Jin-Feng
JF
Song
Ning-Hong
NH
Zhang
Wei
W
Hua
Li-Xin
LX
Wu
Hong-Fei
HF
D000328:Adult; D000368:Aged; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms / Q000209:etiology / Q000235:genetics*; D012306:Risk
C542542:MIRN146 microRNA, human; D035683:MicroRNAs