title: CD9 expression can be used to predict childhood TEL/AML1-positive acute lymphoblastic leukemia: proposal for an accelerated diagnostic flowchart.
abstract: CD9 has been shown to be differentially expressed in childhood TEL/AML1-positive acute lymphoblastic leukemia (ALL). We confirmed this finding in large Affymetrix data sets and in 80 new cases at both RNA and protein levels. Moreover, we showed that mean fluorescence intensity of CD9 by flow cytometry can distinguish TEL/AML1-positive ALL from other BCP-ALL. Using ROC analysis, the most efficient model for predicting TEL/AML1-positive ALL combined CD9 (mean fluorescence intensity <or=20) and CD10 values (positive cells >40%). Finally, we propose a faster procedure for optimizing the diagnosis of childhood BCP-ALL subgroups.
authors.affiliation: Centre National de Recherche Scientifique, Unité Mixte de Recherche 6061 Laboratoire de Génétique et Développement, Equipe Régulation Transcriptionnelle et Oncogenèse, Université de Rennes-1, Rennes Cedex, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Gandemer
authors.forename: Marc
authors.initials: M
authors.lastname: Aubry
authors.forename: Mikael
authors.initials: M
authors.lastname: Roussel
authors.forename: Anne-Gaelle
authors.initials: AG
authors.lastname: Rio
authors.forename: Marie
authors.initials: M
authors.lastname: de Tayrac
authors.forename: Audrey
authors.initials: A
authors.lastname: Vallee
authors.forename: Jean
authors.initials: J
authors.lastname: Mosser
authors.forename: Béatrice
authors.initials: B
authors.lastname: Ly-Sunnaram
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Galibert
mesh_terms: D000465:Algorithms; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002648:Child; D050676:Core Binding Factor Alpha 2 Subunit / Q000032:analysis / Q000235:genetics*; D003661:Decision Support Techniques; D005189:False Positive Reactions; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*; D011379:Prognosis; D060245:Tetraspanin 29; D013997:Time Factors; D014407:Tumor Cells, Cultured; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D050676:Core Binding Factor Alpha 2 Subunit; D008562:Membrane Glycoproteins; D015514:Oncogene Proteins, Fusion; D060245:Tetraspanin 29; C554869:CD9 protein, human; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein