Viral glycosphingolipids induce lytic infection and cell death in marine phytoplankton.
Marine viruses that infect phytoplankton are recognized as a major ecological and evolutionary driving force, shaping community structure and nutrient cycling in the marine environment. Little is known about the signal transduction pathways mediating viral infection. We show that viral glycosphingolipids regulate infection of Emiliania huxleyi, a cosmopolitan coccolithophore that plays a major role in the global carbon cycle. These sphingolipids derive from an unprecedented cluster of biosynthetic genes in Coccolithovirus genomes, are synthesized de novo during lytic infection, and are enriched in virion membranes. Purified glycosphingolipids induced biochemical hallmarks of programmed cell death in an uninfected host. These lipids were detected in coccolithophore populations in the North Atlantic, which highlights their potential as biomarkers for viral infection in the oceans.
Vardi
Assaf
A
Environmental Biophysics and Molecular Ecology Group, Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08901, USA.
Van Mooy
Benjamin A S
BA
Fredricks
Helen F
HF
Popendorf
Kimberly J
KJ
Ossolinski
Justin E
JE
Haramaty
Liti
L
Bidle
Kay D
KD
D017209:Apoptosis; D001267:Atlantic Ocean; D015415:Biomarkers / Q000032:analysis; D020169:Caspases / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry; D049109:Cell Proliferation; D015870:Gene Expression; D005814:Genes, Viral; D006028:Glycosphingolipids / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D054884:Host-Pathogen Interactions; D010788:Photosynthesis; D017862:Phycodnaviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010839:Phytoplankton / Q000737:chemistry / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D012623:Seawater / Q000737:chemistry / Q000821:virology; D015398:Signal Transduction; D014779:Virus Replication
D015415:Biomarkers; D006028:Glycosphingolipids; D020169:Caspases