title: Inflammatory mediators and posterior segment involvement in ocular Behcet disease.
abstract: OBJECTIVE
  To assess the serum level of mediators and their relation with posterior ocular involvement in patients with Behcet disease (BD).
  
  
  METHODS
  This study was a prospective, institutional clinical trial including 43 patients with ocular BD. Patients were divided into two subgroups: those with active posterior segment involvement (Group A) and those with inactive ocular BD (Group B). Group A included patients with posterior uveitis (vitritis or chorioretinal involvement), while Group B included patients with no active intraocular inflammation within the last 3 months. Serum levels of interleukin (IL)-6, IL-8, tumor necrosis factor alpha (TNF-alpha), vascular endothelial growth factor (VEGF), and malondialdehyde (MDA) were measured by sandwich enzyme-linked immunosorbent assay and spectrophotometric assay and compared among the groups by independent sample t test and Mann-Whitney U-test.
  
  
  RESULTS
  Serum levels of the mediators in Group A (IL-6 [23.1 pg/mL], IL-8 [161.1 pg/mL], TNF-alpha [30.5 pg/mL], VEGF [455.9 pg/mL], MDA [10.7 microm]) were found to be significantly higher than those in Group B (IL-6 [12.2 pg/mL], IL-8 [48.4 pg/mL], TNF-alpha [18.6 pg/mL], VEGF [138.0 pg/mL], MDA [5.1 microm]).
  
  
  CONCLUSIONS
  This study demonstrated that increased serum levels of inflammatory mediators including IL-6, IL-8, TNF-alpha, VEGF, and MDA were possibly involved in the development of posterior uveitis in BD.
authors.affiliation: Department of Retinal and Uveal Diseases, Ankara Ulucanlar Eye Research Hospital, Ankara - Turkey. yasemin_oz@yahoo.com
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Ozdamar
authors.forename: Nilufer
authors.initials: N
authors.lastname: Berker
authors.forename: Gul
authors.initials: G
authors.lastname: Bahar
authors.forename: Emel
authors.initials: E
authors.lastname: Soykan
authors.forename: Tolga
authors.initials: T
authors.lastname: Bicer
authors.forename: Seyhan Sonar
authors.initials: SS
authors.lastname: Ozkan
authors.forename: Jale
authors.initials: J
authors.lastname: Karakaya
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D007378:Interleukins / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015866:Uveitis, Posterior / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D055815:Young Adult
chemical_list: D018836:Inflammation Mediators; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde