title: Bone tissue response to titanium implant surfaces modified with carboxylate and sulfonate groups.
abstract: The present study assessed in vivo new bone formation around titanium alloy implants chemically grafted with macromolecules bearing ionic sulfonate and/or carboxylate groups. Unmodified and grafted Ti-6Al-4V exhibiting either 100% carboxylate, or 100% sulfonate, or both carboxylate and sulfonate groups in the percent of 50/50 and 80/20 were bilaterally implanted into rabbit femoral condyle. Neither toxicity nor inflammation were observed for all implants tested. After 4 weeks, peri-implant new bone formation varied as a function of the chemical composition of the titanium surfaces. The percent bone-implant contact (BIC) was the lowest (13.4 +/- 6.3%) for the implants modified with grafted carboxylate only. The value of BIC on the implants with 20% sulfonate (24.6 +/- 5.2%) was significantly (P < 0.05) lower than that observed on 100% sulfonate (38.2 +/- 13.2%) surfaces. After both 4 and 12 weeks post-implantation, the BIC value for implants with more than 50% sulfonate was similar to that obtained with the unmodified Ti-6Al-4V. The grafted titanium alloy exhibiting either 100% sulfonate or carboxylate and sulfonate (50% each) groups promoted bone formation. Such materials are of clinical interest because, they do not promote bacteria adhesion but, they support new bone formation, a condition which can lead to osseointegration of bone implants while preventing peri-implant infections.
authors.affiliation: Laboratoire de Bioingénierie et Biomécanique Ostéoarticulaire, U.M.R.-C.N.R.S. 7052, 10, avenue de Verdun, 75010, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Kerner
authors.forename: V
authors.initials: V
authors.lastname: Migonney
authors.forename: G
authors.initials: G
authors.lastname: Pavon-Djavid
authors.forename: G
authors.initials: G
authors.lastname: Helary
authors.forename: L
authors.initials: L
authors.lastname: Sedel
authors.forename: F
authors.initials: F
authors.lastname: Anagnostou
mesh_terms: D000476:Alkanesulfonates / Q000737:chemistry*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005269:Femur / Q000166:cytology* / Q000601:surgery*; D008297:Male; D008422:Materials Testing; D010316:Particle Size; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000476:Alkanesulfonates; D020099:Coated Materials, Biocompatible; D002244:Carbon; D014025:Titanium