Reduced frequency of regulatory T cells in peripheral blood stem cell compared to bone marrow transplantations.
Peripheral blood stem cell transplantation (PBSCT) is an alternative to bone marrow transplantation (BMT). Although CD4(+)CD25(+)CD127(lo) regulatory T cells (Tregs) have been shown to play important roles in the control of T cell reactivity, the Treg contents of both graft types have not been analyzed comparatively to date. We report herein that Treg frequencies are significantly reduced in PBSC compared to BM transplants. Furthermore, most Tregs from PBSC transplants are CD62L(lo), a phenotype reported to have poor suppressor activity. Both granulocyte-colony stimulating factor (G-CSF) administration and leukapheresis were found to contribute to the loss of CD62L(+) Tregs. Although higher T cell numbers are infused in PBSCT than in BMT, it is possible that the reduced Treg content of PBSC transplants may represent 1 factor contributing to the higher risk of GVHD reported after PBSCT.
Blache
Céline
C
INSERM U905, Faculte de Medecine, 22 Boulevard Gambetta, Rouen, France.
Chauvin
Joe-Marc
JM
Marie-Cardine
Aude
A
Contentin
Nathalie
N
Pommier
Pascal
P
Dedreux
Ingrid
I
François
Stéphanie
S
Jacquot
Serge
S
Bastit
Dominique
D
Boyer
Olivier
O
D017209:Apoptosis / Q000276:immunology; D001853:Bone Marrow / Q000187:drug effects; D016026:Bone Marrow Transplantation; D051858:Forkhead Transcription Factors / Q000032:analysis; D006086:Graft vs Host Disease / Q000276:immunology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D053699:Interleukin-7 Receptor alpha Subunit / Q000032:analysis; D019041:L-Selectin / Q000032:analysis; D007937:Leukapheresis; D017493:Leukocyte Common Antigens / Q000032:analysis; D018655:Lymphocyte Count; D036102:Peripheral Blood Stem Cell Transplantation; D012307:Risk Factors; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000166:cytology* / Q000276:immunology / Q000637:transplantation*; D014019:Tissue Donors
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053699:Interleukin-7 Receptor alpha Subunit; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D017493:Leukocyte Common Antigens