title: [Chemical constituents from herb of Solanum lyratum].
abstract: OBJECTIVE
  To study the chemical constituents of Solanum lyratum, And to explore the anti-tumor effect of some steroidal compounds from it.
  
  
  METHODS
  The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography as well as recrystallization. Their structures were determined on the basis of spectral analysis and physicochemical properties. 3 of the steroidal compounds were tested for their in vitro inhibitory activities in Hep G2 cells.
  
  
  RESULTS
  Tweleve compounds were isolated and identified as diosgenin (1), tigogenin (2), tigogenone (3), diosgenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucuroniduronic acid methyl ester (4), 4-methyl-cholesta-7-ene-3beta-ol (5), acacetin 7-O-rutinoside (6), 1,5-dihydroxy-3-methoxy-7-methy-anthraquinone (7), 1,3,5-trihydroxy-7-methyl-anthracenedione (8), physcion-8-O-beta-D-glucopyranoside (9), soya-cerebroside I (10), isovanillin (11), docosylferulate (12). Compounds 2-4 had no significant inhibition on Hep G2 cells low concentration. With the increased of the concentration, the growth inhibiting effect against the cells increased, too.
  
  
  CONCLUSIONS
  Compounds 2, 3, 5-12 were isolated from the plant for the first time. Compounds 2-4 had growth inhibiting effect against the Hep G2 cells.
authors.affiliation: Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D032322:Solanum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal