title: [Study on the constituents of petroleum ether fraction of Buxus microphylla].
abstract: OBJECTIVE
  To study the chemical constituents from the petroleum ether fraction of Buxus microphylla.
  
  
  METHODS
  The petroleum ether fraction of Buxus microphylla was isolated and identified by silica gel column chromatography. And the anticancer activity of different chemical constituents was measured by MTT reduction test.
  
  
  RESULTS
  Eight compounds were isolated and identified as lupeol (1), butulin (3), beta-sitosterol (4), stigmasterol (5), dibutyl phthalate (6), 3beta, 30-dihydroxy-lup-20 (29) ene (7), daucosterol (8). Compound 7 inhibited KB cells' proliferation in a dose-dependent manner.
  
  
  CONCLUSIONS
  Compounds 2 - 5, 7, 8 are isolated from this genus for the first time. Compound 7 has certainly anticancer effects.
authors.affiliation: Guilin Medical College, Guilin 541004, China. dzhk110@126.com
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Dai
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Su
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Zhang
mesh_terms: D031235:Buxus / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000302:isolation & purification; D004987:Ethers; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification*; D013265:Stigmasterol / Q000737:chemistry / Q000302:isolation & purification*; D000094042:Lupanes
chemical_list: D003993:Dibutyl Phthalate; D004987:Ethers; D053978:Pentacyclic Triterpenes; D012855:Sitosterols; D013265:Stigmasterol; C025473:gamma-sitosterol; C010480:lupeol; D000094042:Lupanes; C011015:lyoniside