[Effects of Yi Fu Ning soft gelatin capsules on reproductive endocrine-immune function of ovariectomized rats].
OBJECTIVE
To investigate the effect of Yi Fu Ning Soft Gelatin Capsules (YFN) on reproductive endocrine-immune function of ovariectomized rats.


METHODS
60 3-month old female Sprague-Dawley rats were used, 50 of them were ovariectomized and randomly divided into 5 groups: ovariectomizy (OVX) group, OVX with diethylstilbestrol tablets (DT) group, OVX with YFN (high dose, middle dose and low dose) group. The others were sham-operated group. The rats were administrated initially in the 4th week after the operation. After drugs had been given for 12 weeks the rats were sacrificed, blood serum hormone, IL-2 content and T lymphocyte subpopulation were detected with methods radioimmunoassay and flow cytometry (FCM).


RESULTS
(1) Compared with sham group, the level of serum E2, Te and P significantly decreased (P < 0.01), FSH, LH content significantly increased; Blood T lymphocyte subpopulation CD3+ cells, CD4+ cells and CD4+/CD8+ ratio significantly decreased, serum IL-2 content also significantly decreased (P < 0.01). (2) Compared with model group, after treated by YFN, the level of serum E2 and P significantly increased (P < 0.01), serum FSH and LH content significantly decreased; T lymphocyte subpopulation CD3+ cells, CD4+ cells and CD4+/CD8+ ratio were improved significantly and serum interleukin-2 (IL-2) content increased significantly.


CONCLUSIONS
YFN can increase serum sexual hormone content,reduce the level of FSH and LH, and improve imbalanced T lymphocyte subpopulation, stimulate IL-2 excretion, which means YFN can regulate inordinate reproductive endocrine-immune network in ovariectomized rats.
Wu
Zhao-Xia
ZX
Department of Biochemistry, Xinxiang Medical University, Xinxiang 453003, China.
Wang
Xiao-Hua
XH
Deng
Hong-Zhu
HZ
D000818:Animals; D002214:Capsules; D030024:Curcuma / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004958:Estradiol / Q000097:blood*; D004967:Estrogens / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D005640:Follicle Stimulating Hormone / Q000097:blood; D006589:Hexestrol / Q000494:pharmacology / Q000627:therapeutic use; D007376:Interleukin-2 / Q000097:blood; D008419:Materia Medica / Q000494:pharmacology* / Q000627:therapeutic use; D010052:Ovariectomy; D010946:Plants, Medicinal / Q000737:chemistry; D011863:Radioimmunoassay; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004967:Estrogens; D007376:Interleukin-2; D008419:Materia Medica; D006589:Hexestrol; D004958:Estradiol; D005640:Follicle Stimulating Hormone