title: BEL-2, an extended-spectrum beta-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa.
abstract: A Pseudomonas aeruginosa isolate recovered in Belgium produced a novel extended-spectrum ss-lactamase, BEL-2, differing from BEL-1 by a single Leu162Phe substitution. That modification significantly altered the kinetic properties of the enzyme, increasing its affinity for expanded-spectrum cephalosporins. The bla(BEL-2) gene was identified from a P. aeruginosa isolate clonally related to another bla(BEL-1)-positive isolate.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Docquier
authors.forename: Filomena
authors.initials: F
authors.lastname: De Luca
authors.forename: Annemie
authors.initials: A
authors.lastname: Verlinde
authors.forename: Louis
authors.initials: L
authors.lastname: Ide
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001530:Belgium; D002510:Cephalosporinase / Q000235:genetics / Q000378:metabolism*; D002511:Cephalosporins / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D002510:Cephalosporinase; D001618:beta-Lactamases; C547757:beta-lactamase BEL-2, Pseudomonas aeruginosa