title: Photo-click immobilization of carbohydrates on polymeric surfaces--a quick method to functionalize surfaces for biomolecular recognition studies.
abstract: Methods to rapidly functionalize specific polymeric surfaces with alkynes, which can subsequently be linked to azide-containing carbohydrates, are presented. The methods comprise two main concepts: azide photoligation and Cu-catalyzed azide-alkyne cycloaddition. 2-Azidoethyl-functionalized alpha-D-mannopyranoside was synthesized and covalently attached to alkyne-functionalized polymeric surfaces using the techniques. The protein recognition properties of the carbohydrate-presenting surfaces were evaluated using quartz crystal microbalance biosensor instrumentation.
authors.affiliation: Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, S-10044, Stockholm, Sweden.
authors.forename: Oscar
authors.initials: O
authors.lastname: Norberg
authors.forename: Lingquan
authors.initials: L
authors.lastname: Deng
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D001672:Biocompatible Materials / Q000032:analysis*; D015374:Biosensing Techniques; D002241:Carbohydrates / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D008766:Methylmannosides / Q000737:chemistry; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D011791:Quartz; D013499:Surface Properties
chemical_list: D000480:Alkynes; D001386:Azides; D001672:Biocompatible Materials; D002241:Carbohydrates; D008766:Methylmannosides; D011108:Polymers; D011506:Proteins; D011791:Quartz; D003300:Copper