Target gene discovery for novel therapeutic agents in cancer treatment.
Target identification of novel therapeutic drugs is pivotal for the establishment of (1) new anticancer regimens, (2) to control side effects of the drugs, and (3) to identify appropriate combinations with established drugs. Here, we describe several in vitro assays applicable to characterize different characteristics of tumor cells. Furthermore, we present a protocol for establishing a reporter gene system for in vivo imaging, allowing for the study of drug effects in small animal models.
Ammerpohl
Ole
O
Clinic for General Surgery and Thoracic Surgery, Division Molecular Oncology, University Hospital of Schleswig, Kiel, Germany.
Tiwari
Sanjay
S
Kalthoff
Holger
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002469:Cell Separation; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004273:DNA, Neoplasm / Q000378:metabolism; D018450:Disease Progression; D015195:Drug Design; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008967:Molecular Biology / Q000639:trends; D009369:Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D013678:Technology, Pharmaceutical / Q000379:methods*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm