Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source.
Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.
Kawarasaki
Tatsuo
T
Shizuoka Prefectural Livestock Institute, Swine and Poultry Research Center, 2780 Nishikata, Kikugawa, Shizuoka 439-0037, Japan. kawarasaki@sp-exp.pref.shizuoka.jp
Uchiyama
Kazuhiko
K
Hirao
Atsushi
A
Azuma
Sadahiro
S
Otake
Masayoshi
M
Shibata
Masatoshi
M
Tsuchiya
Seiko
S
Enosawa
Shin
S
Takeuchi
Koichi
K
Konno
Kenjiro
K
Hakamata
Yoji
Y
Yoshino
Hiroyuki
H
Wakai
Takuya
T
Ookawara
Shigeo
S
Tanaka
Hozumi
H
Kobayashi
Eiji
E
Murakami
Takashi
T
D000818:Animals; D030801:Animals, Genetically Modified / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D023421:Models, Animal; D009928:Organ Specificity; D013552:Swine / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D049452:Green Fluorescent Proteins