Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts.
OBJECTIVE
We investigated the effect of transforming growth factor-beta1 (TGF-beta1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor gamma (PPARgamma) agonists on the effects of TGF-beta1.


METHODS
Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-beta1 and exposed them to the PPARy agonists ciglitazone and 15-deoxy-Delta-prostaglandin J2. The expression of alpha-smooth muscle actin (alpha-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays.


RESULTS
The treatment with TGF-beta1 increased the expression of alpha-SMA and collagen, and expression was markedly attenuated by the PPARy agonists.


CONCLUSIONS
PPARy agonists inhibit the differentiation of TGF-beta1-activated NPDFs and their production of collagen.
Lee
Heung-Man
HM
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul, Korea.
Kang
Hee Jun
HJ
Park
Hyo Hun
HH
Hong
Seok-Chan
SC
Kim
Jin-Kook
JK
Cho
Jae Hoon
JH
D000199:Actins / Q000378:metabolism; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008297:Male; D009298:Nasal Polyps / Q000503:physiopathology*; D047495:PPAR gamma / Q000819:agonists*; D053773:Transforming Growth Factor beta1 / Q000627:therapeutic use*
C541116:ACTA2 protein, human; D000199:Actins; D047495:PPAR gamma; D053773:Transforming Growth Factor beta1; D003094:Collagen