Synthesis, characterization and DNA binding studies of new ruthenium(II)bisterpyridine complexes.
Two new ruthenium(II) complexes, [Ru(itpy)2](PF6)2, 1 and [Ru(bitpy)2](PF6)2 2, were synthesized and characterized by ESI-Mass, UV-Visible, 1H NMR, fluorescence spectroscopy and cyclic voltammetry. Complex 1 has been characterized crystallographically. Interaction of these complexes with CT-DNA has been studied using absorption and CD spectra. Absorption spectral titration and CD spectral measurements show that complex 1 binds with DNA through intercalation. Complex 2 on the other hand shows dual mode of binding to DNA, groove binding as well as intercalation. Photo nuclease activity of these complexes has been studied using agarose gel electrophoresis and both the complexes have been shown to exhibit photonuclease activity. However, complex 1 has been found to show higher DNA cleaving efficiency compared to complex 2.
Sathyaraj
Gopal
G
Chemical Laboratory, Central Leather Research Institute, CSIR, Adyar, Chennai, 600020, India.
Weyhermüller
Thomas
T
Nair
Balachandran Unni
BU
D000042:Absorption; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D044366:Transition Temperature
D009942:Organometallic Compounds; D011725:Pyridines; D012428:Ruthenium; D004247:DNA; D003851:Deoxyribonucleases; C023666:pyridine