title: Expression of Ras homologous B protein in the human scalp skin and hair follicles: hair follicle cycle stages-associated changes.
abstract: BACKGROUND
  RhoB belongs to the Ras homologous (Rho) subfamily which consists of low molecular weight mass GTP-binding proteins. Rho proteins are regulatory molecules that mediate changes in cell shape, contractility, motility and gene expression.
  
  
  OBJECTIVE
  To test the hypothesis that 'RhoB protein is expressed in the human skin and its expression undergoes hair follicle cycle dependent changes'. To test this hypothesis, we examined the expression of RhoB in the normal human skin and hair follicles (HFs) using immunohistochemical methods.
  
  
  METHODS
  A total of 50 normal human scalp skin specimens were obtained from 50 females (age: 53-57 years) undergoing elective cosmetic plastic surgery. The specimens were obtained from both frontal and temporal regions of the scalp. A total of 50 HF, (35 anagen, 10 catagen and 5 telogen) were examined in each case using immunohistologic staining methods. Semiquantitative analysis was done.
  
  
  RESULTS
  RhoB protein was strongly expressed in the various elements of the human scalp skin and hair follicles. In the epidermis, a moderate RhoB immunoreactivity was found in all layers except stratum corneum where RhoB protein was completely absent. In sebaceous glands, a strong RhoB immunoreactivity was detected in all sebaceocytes. In the hair follicles, the expression of RhoB protein showed hair follicle cycle stages-associated changes, i.e. strong expression during anagen, but weak and completely absent expressions during catagen and telogen phases, respectively. Semiquantitative analysis revealed statistically significant high expression values (staining intensity, percentage of positive cells and immunoreactivity scores) in the anagen VI hair follicles compared to either cantagen or telogen ones (p < 0.05). Similarly, RhoB protein expression was significantly high in the stratum basale, stratum spinosum and sebaceous glands compared to stratum granulosum (p < 0.05).
  
  
  CONCLUSIONS
  Here we report, for the first time, the distribution of RhoB protein in the human scalp skin and hair follicles. We also provide the first indication that there are variations in the expression of this protein in the different stages of the hair cycle.
authors.affiliation: Department of Zoology, Faculty of Science, Sohag University, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Adly
authors.forename: Hanan A
authors.initials: HA
authors.lastname: Assaf
authors.forename: Mahmoud Rezk A
authors.initials: MR
authors.lastname: Hussein
mesh_terms: D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D018859:Hair Follicle / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012535:Scalp / Q000378:metabolism*; D020743:rhoB GTP-Binding Protein / Q000378:metabolism*
chemical_list: D020743:rhoB GTP-Binding Protein