title: Backbone resonance assignment of Staphylococcal Enterotoxin H.
abstract: The staphylococcal enterotoxin H (SEH; 217 aa, 25 kD) belongs to a family of superantigens that cause a massive immune response upon simultaneous binding to the T cell receptor (TCR) and the major histocompatibility complex class II. The SEH-TCR interaction is weak and amenable to studies using NMR methodology. Essentially, 2 mg of U{(2)H, (13)C,(15)N}-labeled SEH was used for the complete sequential backbone assignment of SEH at 900 MHz. The protein secondary structure inferred from the chemical shift index (C(alpha) and C(beta)) is in very good agreement with the secondary structure elements of the X-ray structure.
authors.affiliation: Swedish NMR Centre, University of Gothenburg, PO Box 465, 405 30 Gothenburg, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Saline
authors.forename: Vladislav
authors.initials: V
authors.lastname: Orekhov
authors.forename: Karin
authors.initials: K
authors.lastname: Lindkvist-Petersson
authors.forename: B Göran
authors.initials: BG
authors.lastname: Karlsson
mesh_terms: D000595:Amino Acid Sequence; D004768:Enterotoxins / Q000737:chemistry* / Q000235:genetics; D048168:Escherichia coli K12; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics; D018089:Superantigens / Q000737:chemistry* / Q000235:genetics
chemical_list: D004768:Enterotoxins; D011994:Recombinant Proteins; C402489:Staphylococcal enterotoxin H; D018089:Superantigens