title: Depth-resolved measurement of ocular fundus pulsations by low-coherence tissue interferometry.
abstract: A device that allows for the measurement of ocular fundus pulsations at preselected axial positions of a subject's eye is presented. Unlike previously presented systems, which only allow for observation of the strongest reflecting retinal layer, our system enables the measurement of fundus pulsations at a preselected ocular layer. For this purpose the sample is illuminated by light of low temporal coherence. The layer is then selected by positioning one mirror of a Michelson interferometer according to the depth of the layer. The device contains a length measurement system based on partial coherence interferometry and a line scan charge-coupled device camera for recording and online inspection of the fringe system. In-vivo measurements in healthy humans are performed as proof of principle. The algorithms used for enhancing the recorded images are briefly introduced. The contrast of the observed interference pattern is evaluated for different positions of the measurement mirror and at various distances from the front surface of the cornea. The applications of such a system may be wide, including assessment of eye elongation during myopia development and blood-flow-related changes in intraocular volume.
authors.affiliation: Medical University of Vienna, Center for Biomedical Engineering and Physics, Wahringer Strasse 13, Vienna 1090, Austria.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Dragostinoff
authors.forename: René M
authors.initials: RM
authors.lastname: Werkmeister
authors.forename: Martin
authors.initials: M
authors.lastname: Gröschl
authors.forename: Leopold
authors.initials: L
authors.lastname: Schmetterer
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005654:Fundus Oculi; D006801:Humans; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012160:Retina / Q000502:physiology*; D012680:Sensitivity and Specificity