ClC-5 regulates dentin development through TGF-beta1 pathway.
ClC-5 is one of the voltage-dependent chloride channel (ClC) family members. Mutations involving CLCN5 cause an X-linked nephropathy associated with Dent's disease. Some Clcn5 gene knockout (ClC-5 KO) mice have abnormal growth of the teeth; however, the expression and function of ClC-5 during tooth development is still unknown. Herein we report abnormal dentin structure, decreased DSPP and increased TGF-beta1 protein level in ClC-5 KO teeth. In odontoblast-like MDPC-23 cells, the mRNA levels of Tgfb1, Dspp and Dmp-1 were upregulated with Clcn5 RNAi after 48h treatment; whilst there was no change in those of TGF-beta receptor Tgfbr1 and Tgfbr2. We suggest that the dentin changes in ClC-5 KO mice might be a result of increasing TGF-beta1, and the interplay between ClC-5 and TGF-beta1 needs further identified.
Duan
Xiaohong
X
Department of Oral Biology, The Fourth Military Medical University, Xi'an, Shaanxi, China. xhduan@fmmu.edu.cn
Mao
Yong
Y
Yang
Ting
T
Wen
Xuan
X
Wang
Huan
H
Hou
Jin
J
Xue
Yang
Y
Zhang
Rong
R
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018118:Chloride Channels / Q000172:deficiency / Q000378:metabolism*; D003804:Dentin / Q000254:growth & development* / Q000378:metabolism* / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D015870:Gene Expression; D051379:Mice; D018345:Mice, Knockout; D009804:Odontoblasts / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis; D034622:RNA Interference; D012795:Sialoglycoproteins / Q000096:biosynthesis; D014083:Tooth Germ / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015854:Up-Regulation; D000099311:Dentin Sialophosphoprotein
D018118:Chloride Channels; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D053773:Transforming Growth Factor beta1; D000099311:Dentin Sialophosphoprotein; C097503:CLC-5 chloride channel; C513308:Dmp1 protein, mouse