title: Spontaneous aneuploidy and clone formation in adipose tissue stem cells during different periods of culturing.
abstract: Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fluorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.
authors.affiliation: I. M. Sechenov Moscow Medical Academy, Russian Academy of Medical Sciences, Moscow, Russia. OAB422@gmail.com
authors.forename: O A
authors.initials: OA
authors.lastname: Buyanovskaya
authors.forename: N P
authors.initials: NP
authors.lastname: Kuleshov
authors.forename: V A
authors.initials: VA
authors.lastname: Nikitina
authors.forename: E S
authors.initials: ES
authors.lastname: Voronina
authors.forename: L D
authors.initials: LD
authors.lastname: Katosova
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000782:Aneuploidy; D002478:Cells, Cultured; D002877:Chromosomes, Human; D002999:Clone Cells; D006801:Humans; D013234:Stem Cells / Q000166:cytology*