[Biological activity assays and cellular imaging of anti-human sperm protein 17 immunomagnetic nanoparticles].
OBJECTIVE
To prepare anti-Sperm protein 17 (Sp17) immunomagnetic nanoparticles (IMNPs), and make foundation for target diagnosis of ovarian cancer by magnetic resonance imaging.


METHODS
The anti-human Sp17 IMNPs were prepared by grafting anti-Sp17 antibodies on the surface of chitosan-coated magnetic nanoparticles (MNPs) using the linker of EDC/NHS (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide/N-hydroxysuccinimide). The morphology and properties of the nanoparticles were characterized by transmission electronic microscopy (TEM), the conjugation of the antibodies was evaluated by native-polyacrylamide gel electrophoresis, the immunologic activity of IMNPs was evaluated by enzyme linked immunosorbent assay (ELISA). A set of in vitro magnetic resonance imaging (MRI) experiments were performed after incubated the IMNPs with human Sp17 gene transfected ovarian cancer HO-8910 cells.


RESULTS
We had successfully grafted the MNPs with anti-Sp17 antibody and the IMNPs kept good bioactivity. The MRI showed that the IMNPs were targeted successfully to the positive cells, and no obviously non-specific adsorption was observed.


CONCLUSIONS
The anti-Sp17 IMNPs with good specificity can used for further study of ovarian cancer target therapy.
Liu
Qun
Q
Institute of Clinical Laboratory Medicine, PLA, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.
Ge
Yu-qing
YQ
Li
Fang-qiu
FQ
Zhang
Shi-xin
SX
Gu
Ning
N
Wang
Zhong-qiu
ZQ
Lu
Guang-ming
GM
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000276:immunology*; D045744:Cell Line, Tumor; D016764:Cell Polarity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D008565:Membrane Proteins; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging; D053758:Nanoparticles
D000906:Antibodies; D000954:Antigens, Surface; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C090172:SPA17 protein, human