title: Alishewanella agri sp. nov., isolated from landfill soil.
abstract: Strain BL06(T) was isolated from landfill soil in Pohang, Korea. Strain BL06(T) is Gram-negative, aerobic, non-motile and rod-shaped. For growth, the NaCl range is 0-6 % (w/v), the temperature range is 10-44 degrees C and the pH range is 5.5-12.0. Based on the 16S rRNA gene and gyrase B (gyrB) gene sequences, phylogenetic analysis showed that strain BL06(T) is associated with the genus Alishewanella and related closely to the type strains of Alishewanella species (98.8 % 16S rRNA gene sequence similarity to Alishewanella aestuarii, 98.7 % to Alishewanella fetalis and 98.5 % to Alishewanella jeotgali). Physiological and biochemical tests verified that strain BL06(T) is genotypically and phenotypically different from previously described species in the genus Alishewanella. DNA-DNA hybridization experiments showed that relatedness between the genomic DNA of strain BL06(T) and type strains of other Alishewanella species is <41 %. These findings suggest strongly that the strain represents a novel species, despite high 16S rRNA gene sequence similarity between strain BL06(T) and related strains. Therefore, strain BL06(T) (=KCTC 22400(T)=JCM 15597(T)) is proposed to represent a novel species in the genus Alishewanella, named Alishewanella agri sp. nov.
authors.affiliation: University of Science and Technology, Biological Resources Center, KRIBB, Daejeon 305-333, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Ewha Woman's University, Seoul 120-750, Republic of Korea.
authors.forename: Seon Kyung
authors.initials: SK
authors.lastname: Jo
authors.affiliation: University of Science and Technology, Biological Resources Center, KRIBB, Daejeon 305-333, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.affiliation: University of Science and Technology, Biological Resources Center, KRIBB, Daejeon 305-333, Republic of Korea.|Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
mesh_terms: D034241:Alteromonadaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase