Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells.
The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.
Shen
Yan Nan
YN
Department of Biology, Kyung Hee University, Seoul, Korea.
Kim
Yoon Mo
YM
Yun
Cheol-Heui
CH
Moon
Yang Soo
YS
Kim
Sang Hoon
SH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics*
D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger