Chemical composition and antimicrobial and antioxidant activities of Mentha (longifolia L. and viridis) essential oils.
The study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [GC-FID] and gas chromatography/mass spectrometry [GC-MS]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [ABTS] assays) and antimicrobial (Gram-positive and Gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of Mentha longifolia L. and Mentha viridis. GC-MS analysis revealed that M. longifolia was constituted by pulegone (54.41%) as a major component followed by isomenthone (12.02%), 1,8-cineole (7.41%), borneol (6.85%), and piperitenone oxide (3.19%). M. viridis was rich in carvone (50.47%), 1,8-cineole (9.14%), and limonene (4.87%). The antioxidant activity by ABTS assay showed IC(50) values of 476.3 +/- 11.7 and 195.1 +/- 4.2 mg/L for M. longifolia and M. viridis, respectively, the DPPH assays have resulted in a moderate IC(50) (>8000 mg/L and 3476.3 +/- 133 mg/L for M. longifolia and M. viridis, respectively). Antimicrobial activity showed that Listeria monocytogenes and Klebsiella pneumoniae bacteria were more inhibited by the 2 essential oils tested. Escherichia coli was least susceptible. A strong activity was also observed on fungi and yeasts. Carvone, thymol, and piperitone oxide have not been detected in Tunisian M. longifolia. Camphor is reported for the 1st time for M. viridis. Antioxidant and antibacterial activities were correlated to chemical composition.
Mkaddem
Mounira
M
Laboratoire de Modélisation Analyse et Commande des Systèmes, Ecole Natl d'Ingénieurs de Gabès, Rue Omar elkhattab-ZRIG-6029 Gabès, Tunisie.
Bouajila
Jalloul
J
Ennajar
Monia
M
Lebrihi
Ahmed
A
Mathieu
Florence
F
Romdhane
Mehrez
M
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000081005:Cyclohexane Monoterpenes; D057168:Distillation; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D027541:Mentha / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects
D000890:Anti-Infective Agents; D000975:Antioxidants; D000081005:Cyclohexane Monoterpenes; D016166:Free Radical Scavengers; D039821:Monoterpenes; D009822:Oils, Volatile; C039648:pulegone; C006923:carvone