title: Role of hydroperoxide lyase in white-backed planthopper (Sogatella furcifera Horváth)-induced resistance to bacterial blight in rice, Oryza sativa L.
abstract: A pre-infestation of the white-backed planthopper (WBPH), Sogatella furcifera Horváth, conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice (Oryza sativa L.) under both laboratory and field conditions. The infestation of another planthopper species, the brown planthopper (BPH) Nilaparvata lugens Stål, did not significantly reduce the incidence of bacterial blight symptoms. A large-scale screening using a rice DNA microarray and quantitative RT-PCR revealed that WBPH infestation caused the upregulation of more defence-related genes than did BPH infestation. Hydroperoxide lyase 2 (OsHPL2), an enzyme for producing C(6) volatiles, was upregulated by WBPH infestation, but not by BPH infestation. One C(6) volatile, (E)-2-hexenal, accumulated in rice after WBPH infestation, but not after BPH infestation. A direct application of (E)-2-hexenal to a liquid culture of Xoo inhibited the growth of the bacterium. Furthermore, a vapour treatment of rice plants with (E)-2-hexenal induced resistance to bacterial blight. OsHPL2-overexpressing transgenic rice plants exhibited increased resistance to bacterial blight. Based on these data, we conclude that OsHPL2 and its derived (E)-2-hexenal play some role in WBPH-induced resistance in rice.
authors.affiliation: National Institute of Agrobiological Sciences, NIAS, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Gomi
authors.forename: Masaru
authors.initials: M
authors.lastname: Satoh
authors.forename: Rika
authors.initials: R
authors.lastname: Ozawa
authors.forename: Yumi
authors.initials: Y
authors.lastname: Shinonaga
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Sanada
authors.forename: Katsutomo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Masaya
authors.initials: M
authors.lastname: Matsumura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Hiroo
authors.initials: H
authors.lastname: Kanno
authors.forename: Kazuya
authors.initials: K
authors.lastname: Akimitsu
authors.forename: Junji
authors.initials: J
authors.lastname: Takabayashi
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000502:physiology*; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D006430:Hemiptera / Q000502:physiology*; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000469:parasitology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D000447:Aldehydes; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C010463:n-hexanal; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase