[Comparative characteristic of intracellular metabolism in neutrophils of healthy adolescents and adults without coronary heart disease].
The study was designed to compare characteristics of intracellular metabolism in neutrophils of healthy adolescents and adults without coronary heart disease (CHD). A total of 45 healthy adolescents aged 8-16 years and 98 adults (18-54 yr) were examined to measure the level of oxygen radicals in neutrophils and evaluate the state of their antioxidative system. The nitroblue tatrasolium test was used for the purpose along with determination of hydrogen peroxide and lactate content, catalase and glutathione reductase activities. Unlike adolescents, adult subjects without CHD showed reduced activity of the antioxidative enzyme glutathione reductase and enhanced activity of oxidative myeloperoxidase. Smoking in adults was associated with neutrophil priming and activation of metabolic processes in these cells in the absence of a significant rise in glutathione reductase activity. It is concluded that age-specific changes of glutathione reductase activity in adults related to a CHD risk factor (smoking) may contribute to the development of oxidative stress.
Kratnov
A E
AE
Khabarova
I V
IV
Kratnov
A A
AA
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002648:Child; D003324:Coronary Artery Disease / Q000097:blood / Q000209:etiology; D005260:Female; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012016:Reference Values; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*; D055815:Young Adult
D000975:Antioxidants; D017382:Reactive Oxygen Species; D019344:Lactic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D005980:Glutathione Reductase