Phosphinothricin-tripeptide biosynthesis: an original version of bacterial secondary metabolism?
Streptomyces viridochromogenes Tü494 produces the herbicide phosphinothricyl-alanyl-alanine (phosphinothricin-tripeptide=PTT; bialaphos). Its bioactive moiety phosphinothricin competitively inhibits bacterial and plant glutamine synthetases. The biosynthesis of PTT includes the synthesis of the unusual amino acid N-acetyl-demethyl-phosphinothricin and a three step condensation via non-ribosomal peptide synthetases. Two characteristics within the PTT biosynthesis make it suitable to study the evolution of secondary metabolism biosynthesis. First, PTT biosynthesis represents the only known system where all peptide synthetase modules are located on separate proteins. This 'single enzyme system' might be an archetype of the multimodular and multienzymatic non-ribosomal peptide synthetases in evolutionary terms. The second interesting feature of PTT biosynthesis is the pathway-specific aconitase Pmi that is involved in the supply of N-acetyl-demethyl-phosphinothricin. Pmi is highly similar to the tricarboxylic acid aconitase AcnA. They share 64% identity at the DNA level and both belong to the Iron-Regulatory-Protein/AcnA family. Despite their high sequence similarity, AcnA and Pmi catalyze different reactions and are not able to substitute for each other. Thus, the enzyme pair AcnA/Pmi presents an example of the evolution of a secondary metabolite-specific enzyme from a primary metabolism enzyme.
Schinko
Eva
E
Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Tübingen, Germany.
Schad
Klaus
K
Eys
Sema
S
Keller
Ullrich
U
Wohlleben
Wolfgang
W
D000613:Aminobutyrates / Q000378:metabolism*; D015394:Molecular Structure; D010453:Peptide Synthases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism*
D000613:Aminobutyrates; C003121:phosphinothricin; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase