title: Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
abstract: Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.
authors.affiliation: Department of Biology, University of York, Heslington, York YO10 5YW, UK. mbuckley82@gmail.com
authors.forename: Michael
authors.initials: M
authors.lastname: Buckley
authors.forename: Matthew
authors.initials: M
authors.lastname: Collins
authors.forename: Jane
authors.initials: J
authors.lastname: Thomas-Oates
authors.forename: Julie C
authors.initials: JC
authors.lastname: Wilson
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000829:Animals, Domestic; D001106:Archaeology; D001688:Biological Products / Q000032:analysis; D001842:Bone and Bones / Q000737:chemistry*; D002965:Classification / Q000379:methods*; D016000:Cluster Analysis; D003094:Collagen / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008322:Mammals; D008460:Meat / Q000032:analysis; D008903:Minerals / Q000032:analysis; D015999:Multivariate Analysis; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D052616:Solid Phase Extraction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001688:Biological Products; D008903:Minerals; D010446:Peptide Fragments; D003094:Collagen; C027762:bone meal