[Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration].
OBJECTIVE
To study the feasibility of supercritical fluid extraction (SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.


METHODS
Optimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP-HPLC.


RESULTS
The optimal extraction conditions were pressure as 20 MPa, temperature at 45 degrees C, using ethanol as modifier carrier at the volume of 1 mL x g(-1), and 1.5 hour of extraction time.


CONCLUSIONS
SFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.
Song
Shihua
S
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Jia
Xiaobin
X
Chen
Yan
Y
Wang
Lijing
L
Feng
Liang
L
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004875:Ergosterol / Q000032:analysis / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D020940:Reishi / Q000737:chemistry*
D010936:Plant Extracts; D004875:Ergosterol