BEL-2, an extended-spectrum beta-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa.
A Pseudomonas aeruginosa isolate recovered in Belgium produced a novel extended-spectrum ss-lactamase, BEL-2, differing from BEL-1 by a single Leu162Phe substitution. That modification significantly altered the kinetic properties of the enzyme, increasing its affinity for expanded-spectrum cephalosporins. The bla(BEL-2) gene was identified from a P. aeruginosa isolate clonally related to another bla(BEL-1)-positive isolate.
Poirel
Laurent
L
Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
Docquier
Jean-Denis
JD
De Luca
Filomena
F
Verlinde
Annemie
A
Ide
Louis
L
Rossolini
Gian Maria
GM
Nordmann
Patrice
P
D001530:Belgium; D002510:Cephalosporinase / Q000235:genetics / Q000378:metabolism*; D002511:Cephalosporins / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
D002511:Cephalosporins; D002510:Cephalosporinase; D001618:beta-Lactamases; C547757:beta-lactamase BEL-2, Pseudomonas aeruginosa