title: The interferon antagonistic activities of the V proteins from two strains of Newcastle disease virus correlate with their known virulence properties.
abstract: Newcastle disease virus (NDV) is an avian paramyxovirus that exists as hundreds of strains with widely different virulence properties. The NDV V protein exhibits interferon (IFN) antagonistic activity, which contributes to the virulence of the virus. The IFN-antagonistic activities of the V proteins from the avirulent strain La Sota and the moderately virulent strain Beaudette C (BC) were compared in an assay for the rescue of a recombinant NDV expressing the green fluorescent protein (NDV-GFP). Consistent with the virulence properties of the two viruses, the BC V protein exhibits a 4-fold greater ability to rescue replication of NDV-GFP than the La Sota V protein. Four amino acid differences in the C-terminal region of V, as well as the N-terminal region, contribute to the difference in IFN-antagonistic activity between the two V proteins.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors.forename: Judith G
authors.initials: JG
authors.lastname: Alamares
authors.forename: Subbiah
authors.initials: S
authors.lastname: Elankumaran
authors.forename: Siba K
authors.initials: SK
authors.lastname: Samal
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Iorio
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D002645:Chickens; D007372:Interferons / Q000037:antagonists & inhibitors*; D009522:Newcastle disease virus / Q000276:immunology* / Q000472:pathogenicity*; D014764:Viral Proteins / Q000502:physiology*; D037521:Virulence Factors / Q000502:physiology*
chemical_list: C070065:36 kDa V protein, Newcastle disease virus; D014764:Viral Proteins; D037521:Virulence Factors; D007372:Interferons