title: ACE inhibition by astilbin isolated from Erythroxylum gonocladum (Mart.) O.E. Schulz.
abstract: Erythroxylum species have several traditional uses in different countries, including the treatment of hypertension. The ethanol extract from E. gonocladum aerial parts, a species endemic to the Brazilian cerrado, elicited a concentration-dependent inhibition of angiotensin converting enzyme (ACE) (pIC(50)=4.53+/-0.05). Extract fractionation led to the isolation of two compounds, whose structures were assigned by spectrometric data as astilbin and beta-sitosterol, along with a mixture of palmitic, stearic and linolenic acids. This is the first report on the occurrence of these compounds on E. gonocladum. Astilbin promoted significant ACE inhibition in vitro (pIC(50)=5.86+/-0.33) and its activity did not differ from captopril, when both compounds were assayed at 10 microM concentration.
authors.affiliation: School of Pharmacy, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.
authors.forename: M D
authors.initials: MD
authors.lastname: Lucas-Filho
authors.forename: G C
authors.initials: GC
authors.lastname: Silva
authors.forename: S F
authors.initials: SF
authors.lastname: Cortes
authors.forename: T R
authors.initials: TR
authors.lastname: Mares-Guia
authors.forename: V
authors.initials: V
authors.lastname: Perpétua Ferraz
authors.forename: C P
authors.initials: CP
authors.lastname: Serra
authors.forename: F C
authors.initials: FC
authors.lastname: Braga
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D002216:Captopril; D004305:Dose-Response Relationship, Drug; D030921:Erythroxylaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D012855:Sitosterols / Q000302:isolation & purification
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D005227:Fatty Acids; D044948:Flavonols; D010936:Plant Extracts; D012855:Sitosterols; C099069:astilbin; C025473:gamma-sitosterol; D002216:Captopril; D007703:Peptidyl-Dipeptidase A