title: Antigen-specific Treg impair CD8(+) T-cell priming by blocking early T-cell expansion.
abstract: Foxp3(+) Treg are crucial for the maintenance of self-tolerance and have been shown to control CD8(+) T-cell effector functions. In addition, Treg are thought to control the priming of CD8(+) T cells, which recognize the same antigens as Treg. Taking advantage of our model of peripheral tolerance induction to influenza hemagglutinin (HA) after HA gene transfer, we found that HA-specific Treg suppress antigen-linked CTL responses through early blockade of CD8(+) T-cell expansion. Confronted with their cognate antigen, Treg expand more rapidly than CD8(+) T cells and are highly suppressive only during the initial stages of immune priming. They nullify HA-specific CD8(+) T-cell responses, local inflammatory responses and rejection of HA transduced cells. When HA gene transfer is performed with extensive tissue inflammation, HA-specific Treg are less effective but still reduce the frequency of newly primed HA-specific CD8(+) T cells and the ensuing frequency of memory CD8(+) T cells. Our results demonstrate that Treg control CTL priming in an antigen-specific manner at the level of T-cell expansion, highlighting how self-reactive Treg could prevent the induction of autoimmune responses through selective blockade of autoreactive T-cell proliferation.
authors.affiliation: Genethon, CNRS FRE, Evry, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Chappert
authors.forename: Marylène
authors.initials: M
authors.lastname: Leboeuf
authors.forename: Philippe
authors.initials: P
authors.lastname: Rameau
authors.forename: Mélanie
authors.initials: M
authors.lastname: Lalfer
authors.forename: Sabine
authors.initials: S
authors.lastname: Desbois
authors.forename: Roland S
authors.initials: RS
authors.lastname: Liblau
authors.forename: Olivier
authors.initials: O
authors.lastname: Danos
authors.forename: Jean M
authors.initials: JM
authors.lastname: Davoust
authors.forename: David-Alexandre
authors.initials: DA
authors.lastname: Gross
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D064532:Histocompatibility Antigen H-2D; D007156:Immunologic Memory / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D006183:H-2 Antigens; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D064532:Histocompatibility Antigen H-2D