Specific immobilization of influenza A virus on GaAs (001) surface.
In the quest for the development of an all-optical biosensor for rapid detection and typing of viral pathogens, we investigate biosensing architectures that take advantage of strong photoluminescence emission from III-V quantum semiconductors (QS). One of the key elements in the development of such a biosensor is the ability to attach various analytes to GaAs--a material of choice for capping III-V QS of our interest. We report on the study of biofunctionalization of GaAs (001) with polyethylene-glycol (PEG) thiols and the successful immobilization of influenza A virus. A diluted solution of biotinylated PEG thiols in OH-terminated PEG thiols is used to form a network of sites for the attachment of neutravidin. Biotinylated polyclonal influenza A antibodies are applied to investigate the process of the immobilization of inactivated influenza A virus. The successful immobilization is demonstrated using atomic force microscopy and fluorescence microscopy measurements.
Duplan
Valerie
V
Université de Sherbrooke, Department of Electrical and Computer Engineering, Faculty of Engineering, Sherbrooke, Québec J1K2R1, Canada.
Miron
Yannick
Y
Frost
Eric
E
Grandbois
Michel
M
Dubowski
Jan J
JJ
D000268:Adhesiveness; D001152:Arsenicals / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D005708:Gallium / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D009980:Influenza A virus / Q000737:chemistry* / Q000502:physiology*; D013499:Surface Properties
D001152:Arsenicals; D001672:Biocompatible Materials; C043055:gallium arsenide; D005708:Gallium