title: Safety and functional aspects of preselected enterococci for probiotic use in Iberian dry-fermented sausages.
abstract: The purpose of this study was to investigate enterococci for potential probiotic use in Iberian dry-fermented sausages. A total of 15 strains isolated from Iberian dry-fermented sausages, human feces, and pig feces were evaluated for their safety and functional characteristics including biogenic amine (BA) production, antibiotic susceptibility, hemolysis, virulence determinants, cell adhesion, and antimicrobial activity against foodborne pathogens. The strain Enterococcus faecium SE906 was able to establish itself on the intestinal epithelium, inhibiting such pathogenic bacteria as Listeria monocytogenes in vitro. This strain was also considered safe to be used for its low aminogenic potential, and its antibiotic resistance pattern and virulence determinants, being identified as a potential probiotic meat starter culture suitable for manufacture of dry-fermented Iberian sausages.
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Univ de Extremadura, 06071 Badajoz, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Ruiz-Moyano
authors.forename: Alberto
authors.initials: A
authors.lastname: Martín
authors.forename: María José
authors.initials: MJ
authors.lastname: Benito
authors.forename: Emilio
authors.initials: E
authors.lastname: Aranda
authors.forename: Rocío
authors.initials: R
authors.lastname: Casquete
authors.forename: María de Guía
authors.initials: Mde G
authors.lastname: Córdoba
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents; D001422:Bacterial Adhesion / Q000502:physiology; D001679:Biogenic Amines / Q000096:biosynthesis; D018938:Caco-2 Cells; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000187:drug effects / Q000472:pathogenicity* / Q000502:physiology*; D016984:Enterococcus faecium / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D019453:Escherichia coli O157 / Q000502:physiology; D005285:Fermentation; D005511:Food Handling / Q000379:methods; D006461:Hemolysis; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology; D008461:Meat Products / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000009:adverse effects* / Q000378:metabolism*; D012475:Salmonella / Q000502:physiology; D012697:Serine Endopeptidases / Q000378:metabolism; D020713:Vancomycin Resistance / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D001679:Biogenic Amines; D037521:Virulence Factors; D012697:Serine Endopeptidases