A protocol for islet isolation from mouse pancreas.
Mouse islets are commonly used in diabetes-related studies. Adequate amounts of good quality islets are prerequisites for a reliable investigation. We describe a protocol for islet isolation from mouse pancreas. Three major manipulations are employed in the islet isolation procedure: in situ pancreas perfusion with collagenase, pancreas digestion and islet purification. The whole procedure takes 30-45 min for each individual mouse. By using this protocol, a reasonable number of islets can be obtained in a relatively short period of time. This protocol has been proven to be practicable and reproducible. It can be easily followed by individuals who do not have previous experience in the related research field.
Li
Dong-Sheng
DS
Hubei Key Laboratory of Embryonic Stem Cell Research, Tai-He Hospital, Yunyang Medical College, Shiyan, Hubei, China.
Yuan
Ya-Hong
YH
Tu
Han-Jun
HJ
Liang
Qing-Le
QL
Dai
Long-Jun
LJ
D000818:Animals; D004210:Dissection / Q000379:methods*; D005374:Filtration / Q000379:methods; D007515:Islets of Langerhans / Q000601:surgery*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID