title: The oxidation status of ALDH3A1 in human saliva and its correlation with antioxidant capacity measured by ORAC method.
abstract: Oxidation status of the salivary aldehyde dehydrogenase (ALDH) was measured in healthy human population using two-assay fluorimetric method and compared with antioxidant capacity (ORAC) in non-smoking and heavy smokers group. Influence of high or low antioxidant diet was also examined. Except for the group of smokers, the salivary ALDH oxidation degree in human saliva was not correlated with antioxidant capacity. Simultaneously direct administration of the antioxidant-containing drug, Fluimucil, resulted in short-term, but statistically significant increase of the reduced (active) form of the enzyme, presumably due to a radical-scavenging activity of the drug.
authors.affiliation: Department of Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., PL-02097 Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bogucka
authors.forename: Joanna
authors.initials: J
authors.lastname: Giebułtowicz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zawada
authors.forename: Piotr
authors.initials: P
authors.lastname: Wroczyński
authors.forename: Jacek
authors.initials: J
authors.lastname: Wierzchowski
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrzak
authors.forename: Piotr
authors.initials: P
authors.lastname: Piekarczyk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Romanowska
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D004032:Diet; D005260:Female; D005470:Fluorometry / Q000379:methods; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012463:Saliva / Q000201:enzymology* / Q000378:metabolism; D012907:Smoking / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase; D000111:Acetylcysteine