title: [HPLC determination of rhynchophylline and isorhynchophylline in Anshen Yangxue oral liquid].
abstract: OBJECTIVE
  To establish the method for determining rhynchophylline and isorhynchophylline in Anshen Yangxue oral liquid.
  
  
  METHODS
  Rhynchophylline and isorhynchophylline were determined by HPLC. The chromatographic conditions were composed of Kromasil C18 column (5 microm, 4.6 mm x 250 mm), a mixture of methanol and water (55: 45) as mobile phase with 0.01 mol/L triehthylamine, adjusted with acetic acid to pH 7.5. The UV detection wavelength of rhynchophylline and isorhynchophylline was 254 nm.
  
  
  RESULTS
  The average coveries of rhynchophylline and isorhynchophylline were 99.04% and 98.75%, respectively. When rhynchophylline's injection quantity between 0.12 - 1.20 microg and isorhynchophylline's injection quantity between 0.08 -0.80 microg, there were good linear relationships (r1 = 0.9999, r2 = 0.9993).
  
  
  CONCLUSIONS
  This method is simple, sensitive, accurate and can be used for the quality control of Anshen Yangxue oral liquid.
authors.affiliation: Phamaceutical Factory of Guangxi Medical University, Nanning 530021, China. 420882554@QQ.com
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Zong-He
authors.initials: ZH
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Long
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Xin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D026121:Indole Alkaloids / Q000032:analysis*; D000078183:Oxindoles; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D032070:Uncaria / Q000737:chemistry*
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids; D000078183:Oxindoles; C052714:rhyncophylline