Detection of 5-lipoxygenase activity in the liverwort Marchantia polymorpha L.
We detected 5-LOX (arachidonate 5-lipoxygenase) in the homogenate of Marchantia polymorpha by spectrophotometry and mass spectrometry. LC/MS/MS analysis indicated that the liverwort 5-LOX produced 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) with arachidonic acid as a substrate. The 5-LOX activity showed a Ca(2+) response, as demonstrated for human 5-LOX. These findings suggest that the liverwort utilizes an arachidonate cascade in a defense signal response.
Kanamoto
Hirosuke
H
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa, Japan.
Takemura
Miho
M
Ohyama
Kanji
K
D001094:Arachidonate 5-Lipoxygenase / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008297:Male; D047008:Marchantia / Q000201:enzymology*; D013058:Mass Spectrometry; D012995:Solubility; D013053:Spectrophotometry
D001094:Arachidonate 5-Lipoxygenase; D002118:Calcium