A novel herpesvirus in the sanctuary chimpanzees on Ngamba Island in Uganda.
BACKGROUND
Recent studies in non-human primates have led to the discovery of novel primate herpesviruses. In order to get more information on herpesvirus infections in apes, we studied wild born captive chimpanzees.


METHODS
Chimpanzees of the Ngamba island sanctuary, Uganda, were analyzed with pan-herpes polymerase chain reaction (PCR) targeting the herpesvirus DNA polymerase gene and the glycoprotein B gene. The obtained sequences were connected by long-distance PCR, and analyzed phylogenetically.


RESULTS
Twenty-one of 40 individuals were infected with members of the Gammaherpesvirinae, two of them with a novel member of this subfamily. Phylogenetically, the novel virus fell into a clade of primate rhadinoviruses and the Kaposi sarcoma herpesvirus (human herpesvirus 8), representing a third distinct rhadinovirus in chimpanzees.


CONCLUSIONS
Non-human primates harbor several herpesviruses many of which are still unknown. This has implications to management of primates in sanctuaries requiring continuous updates on the management protocols to deal with potential occupational pathogens.
Mugisha
L
L
Chimpanzee Sanctuary & Wildlife Conservation Trust (CSWCT), Entebbe, Uganda.
Leendertz
F H
FH
Opuda-Asibo
J
J
Olobo
J O
JO
Ehlers
B
B
D000595:Amino Acid Sequence; D000818:Animals; D000836:Animals, Zoo; D018420:Ape Diseases / Q000821:virology*; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D014454:Uganda; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins