Deletion of the WWOX gene and frequent loss of its protein expression in human osteosarcoma.
To evaluate the role of WWOX gene in human osteosarcoma, array comparative genomic hybridization on 10 frozen osteosarcoma specimens and immunohistochemical staining of 55 formalin-fixed and paraffin-embedded tissues for WWOX was performed. Deletion of the WWOX gene was observed in 3 of 10 samples and the WWOX protein was undetectable in 34 of 55 osteosarcomas. This is the first investigation of the role of WWOX gene in human osteosarcoma. The WWOX gene deletion, loss of its protein expression, and lack of correlation of WWOX expression with patient survival suggest loss of WWOX expression is an early event in the pathogenesis of osteosarcoma and the phenotypic results of its deletion do not imminently result in patient death.
Yang
Jilong
J
Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Hospital and Institute, China.
Cogdell
David
D
Yang
Da
D
Hu
Limei
L
Li
Haixin
H
Zheng
Hong
H
Du
Xiaoling
X
Pang
Yi
Y
Trent
Jonathan
J
Chen
Kexin
K
Zhang
Wei
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D044127:Epigenesis, Genetic; D005260:Female; D017353:Gene Deletion; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics*; D000074584:WW Domain-Containing Oxidoreductase
D025521:Tumor Suppressor Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human