Initial bioadhesion on dental materials as a function of contact time, pH, surface wettability, and isoelectric point.
The adsorption of bovine serum albumin (BSA) on surfaces of dental enamel and of dental materials was investigated by scanning force spectroscopy. This method provides adhesion forces which can be measured as a function of contact time between protein and surface, pH, wettability, and isoelectric point of the surface. Whereas the chosen ceramic and composite materials resemble very well the adhesion on natural enamel, a much stronger adhesion was found for the more hydrophobic surfaces, that is, gold, titanium, poly(methyl methacrylate) (PMMA), and poly(tetrafluoroethylene) (PTFE). On hydrophilic surfaces, adhesion is mainly influenced by the electrostatic forces between protein and surface. However, the conformational change of BSA at pH values above pH 8 has to be taken into account. On the very hydrophobic PTFE surface, the special interface structure between PTFE and water plays an important role which governs BSA adhesion.
Müller
Christine
C
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, D-67663 Kaiserslautern, Germany.
Lüders
Anne
A
Hoth-Hannig
Wiebke
W
Hannig
Matthias
M
Ziegler
Christiane
C
D000327:Adsorption; D003738:Dental Cements; D003764:Dental Materials; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D017155:Wettability
D003738:Dental Cements; D003764:Dental Materials; D012710:Serum Albumin, Bovine; D006046:Gold; D011138:Polytetrafluoroethylene; D019904:Polymethyl Methacrylate; D014025:Titanium