title: Acetylcholinesterase as a potential target of acute neurotoxic effects of lindane in rats.
abstract: The aim of our study was to investigate the possible involvement of acetylcholinesterase (AchE) in mediating the early phase of acute lindane neurotoxicity in rats. Male Wistar rats (n = 48) were divided into following groups: 1. control, saline-treated group; 2. dimethylsulfoxidetreated group; 3. group that received lindane dissolved in dimethylsulfoxide, in a dose of 8 mg/kg intraperitoneally. Eight animals from each group were sacrificed 0.5 and 4 h after treatment and brain samples were prepared for further analysis. AchE activity (mitochondrial and synaptosomal fraction) was determined in cerebral cortex, thalamus, hippocampus and nc. caudatus spectrophotometrically. A significant increase in mitochondrial AchE activity was detected in cortex and nc. caudatus of lindane-treated animals 0.5 h after administration (p < 0.05). This rise was sustained in nc. caudatus within 4 h after treatment (p < 0.05). In contrast, activity of synaptosomal AchE fraction was significantly increased only in thalamus 4 h after lindane administration (p < 0.05). An increase in AchE activity may be involved in mediating acute neurotoxic effects of lindane, at least in some brain structures in rats.
authors.affiliation: Department of Pathophysiology, School of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Vucević
authors.forename: Natasa
authors.initials: N
authors.lastname: Petronijević
authors.forename: Nevena
authors.initials: N
authors.lastname: Radonjić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rasić-Marković
authors.forename: Dusan
authors.initials: D
authors.lastname: Mladenović
authors.forename: Tatjana
authors.initials: T
authors.lastname: Radosavljević
authors.forename: Dragan
authors.initials: D
authors.lastname: Hrncić
authors.forename: Dragan
authors.initials: D
authors.lastname: Djurić
authors.forename: Veselinka
authors.initials: V
authors.lastname: Susić
authors.forename: Macut
authors.initials: M
authors.lastname: Djuro
authors.forename: Olivera
authors.initials: O
authors.lastname: Stanojlović
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008297:Male; D009420:Nervous System / Q000187:drug effects* / Q000201:enzymology*; D010575:Pesticides / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010575:Pesticides; D001556:Hexachlorocyclohexane; D000110:Acetylcholinesterase