title: Effects of the veterinary pharmaceutical ivermectin on soil invertebrates in laboratory tests.
abstract: As part of the risk assessment of veterinary pharmaceuticals, the potential impact of these chemicals on soil ecosystems has to be determined according to European law. However, almost no data from standardized laboratory tests are available. Therefore, in the EU FP6 ERAPharm, the effects of the widely used veterinary pharmaceutical ivermectin, an anthelminthic, were studied in chronic laboratory tests performed according to OECD (Organisation for Economic Co-operation and Development) and ISO (International Organization for Standardization) guidelines. In detail, three soil invertebrate species--the earthworm Eisenia fetida, the springtail Folsomia candida, and the predatory mite Hypoaspis aculeifer--were tested. The nominal concentrations of ivermectin mixed into the test substrate artificial soil was verified using residue analysis, which indicated that the test substance is persistent for at least up to 28 days. As expected when considering the mode of action of this substance, survival and reproduction of collembolans were clearly affected [LC(50) = 8.4 mg/kg soil dry weight (dw); NOEC(repro) = 0.3 mg/kg soil (dw)]. Predatory mites reacted less sensitively [LC(50) > or = 31.6 mg/kg soil (dw); NOEC(repro) = 3.2 mg/kg soil (dw)]. Earthworm survival and reproduction were affected in the same order of magnitude as the predatory mites [LC(50) > or = 10 mg/kg soil (dw); NOEC(repro) = 2.5 mg/kg soil (dw)]. These results are in good agreement with the few data known from nonstandardized tests for the same or related soil invertebrate species. The results of these tests indicate that the effects of ivermectin on soil invertebrates--in particular, collembolans--cannot be excluded at field-relevant concentrations, as determined in a risk assessment according to VICH guidelines. More sophisticated higher-tier tests (e.g., in multispecies or semifield test systems) are recommended in order to assess the potential risk more accurately.
authors.affiliation: ECT Oekotoxikologie GmbH, Böttgerstr. 2-14, 65439, Flörsheim, Germany. j-roembke@ect.de
authors.forename: J
authors.initials: J
authors.lastname: Römbke
authors.forename: K A
authors.initials: KA
authors.lastname: Krogh
authors.forename: T
authors.initials: T
authors.lastname: Moser
authors.forename: A
authors.initials: A
authors.lastname: Scheffczyk
authors.forename: M
authors.initials: M
authors.lastname: Liebig
mesh_terms: D000818:Animals; D017753:Ecosystem; D007313:Insecta / Q000187:drug effects / Q000502:physiology; D007306:Insecticides / Q000633:toxicity*; D007448:Invertebrates / Q000187:drug effects* / Q000502:physiology; D007559:Ivermectin / Q000633:toxicity*; D008136:Longevity / Q000187:drug effects; D008925:Mites / Q000187:drug effects / Q000502:physiology; D009835:Oligochaeta / Q000187:drug effects / Q000502:physiology; D012098:Reproduction / Q000187:drug effects; D018570:Risk Assessment; D012987:Soil; D012989:Soil Pollutants / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D007306:Insecticides; D012987:Soil; D012989:Soil Pollutants; D007559:Ivermectin