title: Orphan nuclear receptor Nur77 is required for the differentiation of C6 glioma cells induced by cholera toxin.
abstract: OBJECTIVE
  To investigate a possible regulator gene involved in the cholera toxin-induced differentiation of rat C6 glioma cells.
  
  
  METHODS
  The global changes in the mRNA expression pattern induced by cholera toxin were analyzed using gene chip microarray. The selected gene was then silenced by RNA interference or overexpressed with an ORF plasmid to determine its necessity in this process.
  
  
  RESULTS
  Nur77, a member of the orphan nuclear receptor family (NR4A), was markedly up-regulated during the process of differentiation. Furthermore, RNAi of nur77 attenuated the induction effect of cholera toxin on C6 cells, whereas overexpression of nur77 led to similarly differentiated behavior, including morphologic and biomarker changes, as well as cell cycle arrest.
  
  
  CONCLUSIONS
  Nur77 participated actively and essentially as an important regulator in the cholera toxin-induced differentiation of C6 cells.
authors.affiliation: Department of Pharmacology, Zhong-shan Medical College, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.forename: Yi-jun
authors.initials: YJ
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Guang-mei
authors.initials: GM
authors.lastname: Yan
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002772:Cholera Toxin / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism*; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation
chemical_list: D000276:Adjuvants, Immunologic; D014408:Biomarkers, Tumor; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D012333:RNA, Messenger; D002772:Cholera Toxin