title: An ELISA based on recombinant spike protein S for the detection of antibodies to transmissible gastroenteritis virus of swine-like canine coronaviruses.
abstract: Recombinant canine coronaviruses, resembling the transmissible gastroenteritis virus of swine (TGEV) in a 5' fragment of the S glycoprotein, have been detected recently and showed to be present in canine populations. The 5' fragment of the S protein (S') of a TGEV-like canine coronavirus (CCoV), strain 174/06, was expressed in an Escherichia coli cell-free system. The purified recombinant polypeptide was employed to develop an ELISA test for the detection of TGEV-like CCoV-specific antibodies in dog sera. Four canine sera positive for TGEV-like CCoV, six sera positive to classical CCoV-II strains and 10 negative control sera were examined. The recombinant S' was not recognized by antibodies to classical CCoV-II, as only sera from dogs infected experimentally with TGEV-like CCoV reacted strongly with the recombinant S' polypeptide whereas dog sera with antibodies to classical CCoV-II did not react. As classical CCoV-II and TEGV-like CCoVs are related antigenically, the recombinant S' ELISA is a useful method to investigate serologically the prevalence of TGEV-like CCoVs in dogs.
authors.affiliation: Department of Veterinary Public Health, University of Bari, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lorusso
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Stella Lucente
authors.initials: SL
authors.lastname: Maria
authors.forename: Paolo
authors.initials: P
authors.lastname: Cordioli
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary*; D017939:Coronavirus, Canine / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics; D044967:Serum / Q000276:immunology; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; C064484:spike protein S, Transmissible gastroenteritis virus