Development of a liquid chromatography-isotope dilution mass spectrometry method for quantification of fentanyl in human plasma.
Fentanyl is a potent analgesic drug in relieving chronic pain in patients. In this report, we present a simple, reliable and sensitive LC-ID/MS method for the quantification of fentanyl in human plasma. LC-ID/MS analysis was carried out on a triple quadrupole mass spectrometer operated in positive electrospray ionization multiple-reaction-monitoring using the transitions m/z 337.6 --> 187.9 for fentanyl and m/z 342.6 --> 187.9 for the internal standard (D5-fentanyl). The calibration curve covered the range 0.02-10 ng/mL. The intra- and inter-batch precision were less than 6.739 and 3.126% for fentanyl and IS, with accuracy from 94.16 to 102.0%. The lower limit of quantification was identifiable and reproducible at 0.02 ng/mL. The validated method offered increased sensitivity and wide linear concentration range. This method was successfully adopted for the evaluation of bioequivalence of two fentanyl transdermal preparations after single dose administration to 20 Chinese pain-patients.
Lu
Chuan
C
Central Laboratory, Shanghai Xuhui Central Hospital, 966 Huaihai Middle Road, Shanghai, China.
Jia
Jing-ying
JY
Gui
Yu-zhou
YZ
Liu
Gang-yi
GY
Shi
Xiao-jin
XJ
Li
Shui-jun
SJ
Yu
Chen
C
D000701:Analgesics, Opioid / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D005283:Fentanyl / Q000097:blood*; D006801:Humans; D010146:Pain / Q000097:blood / Q000188:drug therapy; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000701:Analgesics, Opioid; D005283:Fentanyl