title: On-line solid-phase extraction of large-volume injections coupled to liquid chromatography-tandem mass spectrometry for the quantitation and confirmation of 14 selected trace organic contaminants in drinking and surface water.
abstract: We describe the development and validation of an on-line solid-phase extraction of large-volume injections coupled to liquid chromatography-tandem mass spectrometry method for the simultaneous quantitation and confirmation of 14 selected trace organic contaminants in drinking and surface water. Selected compounds were: anti-infectives (clarithromycin, sulfamethoxazole and trimethoprim), an anticonvulsant (carbamazepine) and its transformation product 10,11-dihydrocarbamazepine, an antihypertensive (enalapril), antineoplastics (cyclophosphamide and methotrexate), herbicides (atrazine, cyanazine, and simazine) and two of their transformation products (deethylatrazine and deisopropylatrazine) and an antiseptic (triclocarban). The breakthrough volume determinations showed that out of all the investigated sorbents, the Strata-X on-line solid-phase extraction column showed the best performance. The method used a load volume of 10.0 mL and was validated using the corresponding matrices, yielding for most compounds, R(2)>0.99. Extraction recoveries ranged from 60 to 109%. The intra- and inter-day precision were <14 and <16%, respectively. The method detection limits ranged from 0.6 to 6 ng L(-1). Matrix effects were in general low. The performance of the on-line method was demonstrated with the analysis of real water samples. The application of alternative techniques of confirmation was also explored using accurate mass measurements on a time-of-flight mass spectrometer and the data-dependent reverse energy ramp scan on a triple quadrupole.
authors.affiliation: Department of Chemistry, Université de Montréal, C.P. 6128, succ. Centre-Ville, Montreal, QC H3C 3J7, Canada.
authors.forename: Araceli
authors.initials: A
authors.lastname: Garcia-Ac
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Segura
authors.forename: Liza
authors.initials: L
authors.lastname: Viglino
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fürtös
authors.forename: Christian
authors.initials: C
authors.lastname: Gagnon
authors.forename: Michèle
authors.initials: M
authors.lastname: Prévost
authors.forename: Sébastien
authors.initials: S
authors.lastname: Sauvé
mesh_terms: D002170:Canada; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D009862:Online Systems / Q000295:instrumentation*; D009930:Organic Chemicals / Q000032:analysis*; D010575:Pesticides / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D013499:Surface Properties; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
chemical_list: D009930:Organic Chemicals; D010575:Pesticides; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical