Biomagnetic glasses: preparation, characterization, and biosensor applications.
In this work, a novel avenue to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities to be used in the design of highly stable, magnetically separable enzyme-based systems was explored. As a model system, immobilization of acetylcholinesterase (AChE) was investigated using biomagnetic glasses composed of a magnetic core with a size tunable porous silica shell. The efficiency of the immobilization was determined by analyzing the biosensing capability of these biomagnetic glasses for the detection of the organophosphorous pesticide paraoxon. Screen printed electrodes with the AChE-biomagnetic glasses showed higher current response and stability than for the free enzyme. The detection limit of the paraoxon biosensor was in the nanomolar range.
Won
Yu-Ho
YH
School of Materials Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Jang
Ho Seong
HS
Kim
Seung Min
SM
Stach
Eric
E
Ganesana
Mallikarjunarao
M
Andreescu
Silvana
S
Stanciu
Lia A
LA
D000110:Acetylcholinesterase; D015374:Biosensing Techniques; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D010261:Paraoxon / Q000032:analysis; D014961:X-Ray Diffraction
D004800:Enzymes, Immobilized; D000110:Acetylcholinesterase; D010261:Paraoxon