Qualitative and quantitative determination of the caffeoylquinic acids on the Korean mountainous vegetables used for chwinamul and their peroxynitrite-scavenging effect.
Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtained from several plants belonging to the Compositae - e.g., Kalimeris yomena, Aster scaber, Solidago virga var. gigantea, Solidago viragaurea var. asiatica, Saussurea grandifolia, Ainsliaea acerifolia - were used for our experiments. Analytical methods for simultaneous determination of the caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-dicaffeoyl-epi-quinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-p-coumaroyl-caffeoylquinic acids) were established for chwinamul. The kinds of constituents were identified from HPLC chromatograms and it was possible to calculate the percentage (w/w) of seven of these compounds in the dried plants and in the extracts. The proportion of caffeoylquinic acids in the extracts ranged from 20.25 to 38.35%. Since it is known that peroxynitrite (ONOO(-))-scavenging is beneficial for amelioration of obesity, diabetes mellitus, atherosclerosis and even Alzheimer's disease, assays for peroxynitrite-scavenging activity were performed on the seven chwinamul plants. Of the tested extracts, the MeOH extract of A. acerifolia had the most potent effect (IC(50) 1.49 +/- 0.68 microg/mL). These results suggest that chwinamul vegetables can be used for treatment or prevention of peroxynitrite-related diseases.
Nugroho
Agung
A
Department of Pharmaceutical Engineering, Sangji University, Wonju, Korea.
Kim
Ki Hyun
KH
Lee
Kang Ro
KR
Alam
Md Badrul
MB
Choi
Jae Sue
JS
Kim
Won-Bae
WB
Park
Hee-Juhn
HJ
D000531:Altitude; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D030421:Peroxynitrous Acid / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D056910:Republic of Korea; D014675:Vegetables / Q000737:chemistry* / Q000254:growth & development
D016166:Free Radical Scavengers; D005609:Free Radicals; C472707:caffeoylquinic acid; D011801:Quinic Acid; D030421:Peroxynitrous Acid