title: Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils.
abstract: In human basophils from different subjects, maximum IgE-mediated histamine release and the level of syk protein expression correlate well. It is not clear when in the basophil's lifetime the set-point for syk expression is reached or how expression levels are determined for a given individual. An examination of syk expression in peripheral blood eosinophils, neutrophils, monocytes, B and T cells, DCs, and NK cells showed that with the exception of T cells, basophils were unique in expressing low levels of syk. No correlations were observed between syk expression in basophils and other types of leukocytes, suggesting a unique mechanism of regulation for basophils. The expression level of syk in CD34+ progenitors was approximately 11-fold higher than in peripheral blood basophils, and it remained at this level during maturation of the cells in IL-3 to a cell with characteristics of peripheral blood basophils. Down-regulation of syk expression in the culture-derived basophils was induced by culturing under conditions of chronic aggregation of FcepsilonRI. Syk was down-regulated to peripheral blood basophil levels in 50% of the cells. Despite the chronic aggregation of FcepsilonRI, the cells retained the same expression of FcepsilonRI, histamine content, and morphological staining of granules as cells not experiencing chronic aggregation. These results suggest that chronic stimulation through FcepsilonRI during basophil maturation might be a mechanism for down-regulating syk expression, while retaining other characteristics associated with mature peripheral blood basophils.
authors.affiliation: Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
authors.forename: Susan S
authors.initials: SS
authors.lastname: Ishmael
authors.forename: Donald W
authors.initials: DW
authors.lastname: MacGlashan
mesh_terms: D018952:Antigens, CD34; D001491:Basophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006636:Histamine Release / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000276:immunology; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D000072377:Syk Kinase
chemical_list: D018952:Antigens, CD34; D047908:Intracellular Signaling Peptides and Proteins; D017455:Receptors, IgE; D007073:Immunoglobulin E; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase