Acanthopanax senticosides B ameliorates oxidative damage induced by hydrogen peroxide in cultured neonatal rat cardiomyocytes.
Acanthopanax senticosides B is a monomer of Acanthopanax senticosus saponins. Previous reports showed that Acanthopanax senticosus saponins exhibit antioxidant action and cardiac protection. However, whether Acanthopanax senticosides B has cardiac protection remains unknown. In this study, we investigated the effect of Acanthopanax senticosides B on oxidative damage induced by hydrogen peroxide (H(2)O(2)) of cardiomyocytes. As revealed from the results, when the cardiomyocytes were exposed to H(2)O(2) for designed time and concentration, the percentage of survival cells was down significantly, the activity of lactate dehydrogenase (LDH) and the content of cellular malondialdehyde (MDA) were increased markedly. These results indicated that the cells were subjected to oxidative damage. Treatment with Acanthopanax senticosides B prior to H(2)O(2) (100 microM) exposure can increase cell viability significantly, lessen H(2)O(2)-induced cardiomyocyte morphological change, and inhibit augmentation of LDH activity in culture media and cellular MDA content markedly. Our results demonstrated that Acanthopanax senticosides B (400 microg/ml and 200 microg/ml) can protect cells against oxidative injury of H(2)O(2) (100 microM). Furthermore, the activities of superoxide dismutase (SOD), glutathione peroxidase, catalase and the content of reduced glutathione (GSH) of cardiomyocytes were also raised by Acanthopanax senticosides B. Taken together, the study implicated that Acanthopanax senticosides B protects cardiomyocytes against oxidative-stress injury induced by H(2)O(2) through reduction of lipid peroxidation and enhancement of the activity of antioxidant defense.
Liang
Qiming
Q
Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Yu
Xiaofeng
X
Qu
Shaochun
S
Xu
Huali
H
Sui
Dayuan
D
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D028001:Eleutherococcus / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*
D000975:Antioxidants; D012503:Saponins; C548348:senticoside B; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D005978:Glutathione