[Correlation of XPD gene with susceptibility to gastric cancer].
OBJECTIVE
Mutations in DNA repair system are related to carcinogenesis. This study was to evaluate the correlations of polymorphisms and haplotypes of XPD gene with individual susceptibility to gastric cancer.


METHODS
Genomic DNA were extracted from peripheral blood leukocytes of 207 gastric cancer patients and 212 healthy controls. Genotypes at codon 312 and codon 751 polymorphic sites were identified by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) or polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP), respectively.


RESULTS
At codon 312, the frequency of GA or AA genotype was higher in the gastric cancer patients than in the healthy controls (P<0.01, OR=3.41, 95% CI: 2.06-4.79; P<0.01, OR=3.47, 95% CI: 1.39-8.68). No significant difference was found in the distribution of the polymorphism at codon 751 between the two groups (P>0.05). By the haplotype AA (codon 312A-codon 751A) analysis, the frequency of heterozygote (-/AA) or homozygote (AA/AA) was higher in the patients than in the controls (P<0.01,OR=2.81, 95% CI:1.82-4.34;P=0.02,OR=3.92, 95% CI:1.31-11.70, respectively). Whereas there were no significant differences of the other three haplotypes between the patients and the controls (P>0.05).


CONCLUSIONS
The polymorphism of XPD at codon 312 might contribute to the etiology of gastric cancer. The haplotype AA (codon 312A-codon 751A) would be a critical risk factor of the susceptibility to gastric cancer.
Zhang
Chuan-Zhen
CZ
Department of Digestive Diseases, Medical College, Shandong University, Shandong, P. R. China.
Chen
Zi-Ping
ZP
Xu
Chang-Qing
CQ
Ning
Tao
T
Li
Dan-Ping
DP
Hou
Rui-Ping
RP
D000328:Adult; D000368:Aged; D003062:Codon; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human