[Isolation and pharmacological properties of analgesic fraction from venom of Naja Naja atra].
OBJECTIVE
To separate main analgesic fraction from venom of Guangdong Naja naja atra, to establish the basis for the using of Naja naja atra and find new analgesic fraction.


METHODS
Affinity chromatography and size exclusion were used to isolate the analgesic fraction from the venom of Naja naja atra, and then to determine its properties by biochemical methods, such as SDS-polyacryamide gel electrophoresis ( SDS-PAGE), HPLC and Mole-toff.


RESULTS
HPLC showed its relative purity was 95% (HPLC)and Mw was 6741. 236 Da. We observed that peripheral administration of neurotoxin strongly reduced the mechanical allogynia and thermal hyperalgesia for 24 hours, associated with this neuropathy (L5 spinal nerve ligation).


CONCLUSIONS
The fraction from venom of Naja naja atra has significant analgesic effect and it is worth further developing.
Liang
Ying-Xia
YX
Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Han
Li-Ping
LP
Jiang
Wei-Jian
WJ
Zhao
Shu-Jin
SJ
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D004546:Elapid Venoms / Q000737:chemistry*; D017815:Elapidae; D007274:Injections, Intraperitoneal; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D009437:Neuralgia / Q000188:drug therapy* / Q000473:pathology; D017288:Pain Threshold / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000700:Analgesics; D004546:Elapid Venoms; D008419:Materia Medica