title: Exposure assessment of PM2.5 and urinary 8-OHdG for diesel exhaust emission inspector.
abstract: Animal studies have shown exposure to diesel exhaust particles (DEPs) to induce production of reactive oxygen species (ROSs) and increase levels of 8-hydroxydeoxyquanosine (8-OHdG). Controversial results have been obtained regarding the effects of workplace exposure on urinary 8-OHdG level. This study assessed concentrations of environmental PM(2.5) in DEP (DEP(2.5)), personal DEP(2.5) and urinary 8-OHdG of diesel engine exhaust emission inspector (inspector) at a diesel vehicle emission inspection station (inspection station). The analysis specifically focuses on the factors that influence inspector urinary 8-OHdG. Repeated-measures study design was used to sample for five consecutive days. A total of 25 environmental PM(2.5) measurements were analyzed at 5 different locations by using a dichotomous sampler, and a total of 55 personal PM(2.5) measurements were analyzed from inspectors by using PM(2.5) personal sampler. During the sampling period, a total of 110 pre- and post-work urine samples from inspectors, and 32 samples from the control group were collected. Following age and sex matching between the inspectors and the control group, levels of urinary 8-OHdG were analyzed. Environmental and personal concentrations of DEP(2.5) were 107.25+/-39.76 (mean+/-SD) and 155.96+/-75.70 microg/m(3), respectively. Also, the concentration of urinary 8-OHdG differed significantly between inspector and control non-smokers, averaging 14.05+/-12.71 and 6.58+/-4.39 microg/g creatinine, respectively. Additionally, urinary 8-OHdG concentrations were associated with diesel exposure after controlling for smoking and cooking at home. Compared with the control group, the inspector displayed significantly increased levels of urinary 8-OHdG. Diesel exhaust is the single pollutant involved in the exposure of DEP(2.5) at the inspection station, as confirmed by the final results.
authors.affiliation: Institute of Environmental and Occupational Health Sciences, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., Beitou, Taipei, Taiwan.
authors.forename: Mei-Wen
authors.initials: MW
authors.lastname: Lee
authors.forename: Mei-Lien
authors.initials: ML
authors.lastname: Chen
authors.forename: Shih-Chun Candice
authors.initials: SC
authors.lastname: Lung
authors.forename: Chung-Jung
authors.initials: CJ
authors.lastname: Tsai
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Yin
authors.forename: I-Fang
authors.initials: IF
authors.lastname: Mao
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis*; D003296:Cooking; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006798:Housing; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D052638:Particulate Matter / Q000652:urine*; D012189:Retrospective Studies; D018570:Risk Assessment; D012907:Smoking; D001335:Vehicle Emissions / Q000032:analysis*; D017132:Workplace
chemical_list: D000395:Air Pollutants, Occupational; D052638:Particulate Matter; D001335:Vehicle Emissions; C024829:8-hydroxyguanine; D006147:Guanine