Genetic mapping of quantitative trait loci associated with beta-amylase and limit dextrinase activities and beta-glucan and protein fraction contents in barley.
High malting quality of barley (Hordeum vulgare L.) relies on many traits, such as beta-amylase and limit dextrinase activities and beta-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for beta-glucan and limit dextrinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to beta-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.
Wei
Kang
K
Department of Agronomy, Zhejiang University, Hangzhou 310029, China.
Xue
Da-wei
DW
Huang
You-zong
YZ
Jin
Xiao-li
XL
Wu
Fei-bo
FB
Zhang
Guo-ping
GP
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D017343:Genes, Plant; D008040:Genetic Linkage; D014644:Genetic Variation; D005838:Genotype; D006026:Glycoside Hydrolases / Q000235:genetics*; D001467:Hordeum / Q000378:metabolism*; D010641:Phenotype; D040641:Quantitative Trait Loci; D012822:Silicon Dioxide / Q000737:chemistry; D001614:beta-Amylase / Q000235:genetics*; D047071:beta-Glucans / Q000378:metabolism*
D047071:beta-Glucans; D012822:Silicon Dioxide; D006026:Glycoside Hydrolases; D001614:beta-Amylase; C100181:pullulanase