title: Modulation of oxidative phosphorylation machinery signifies a prime mode of anti-ageing mechanism of calorie restriction in male rat liver mitochondria.
abstract: Mitochondria being the major source and target of reactive oxygen species (ROS) play a crucial role during ageing. We analyzed ageing and calorie restriction (CR)-induced changes in abundance of rat liver mitochondrial proteins to understand key aspects behind the age-retarding mechanism of CR. The combination of blue-native (BN) gel system with fluorescence Difference Gel Electrophoresis (DIGE) facilitated an efficient analysis of soluble and membrane proteins, existing as monomers or multi-protein assemblies. Changes in abundance of specific key subunits of respiratory chain complexes I, IV and V, critical for activity and/or assembly of the complexes were identified. CR lowered complex I assembly and complex IV activity, which is discussed as a molecular mechanism to minimize ROS production at mitochondria. Notably, the antioxidant system was found to be least affected. The GSH:GSSG couple could be depicted as a rapid mean to handle the fluctuations in ROS levels led by reversible metabolic shifts. We evaluated the relative significance of ROS generation against quenching. We also observed parallel and unidirectional changes as effect of ageing and CR, in subunits of ATP synthase, cytochrome P450 and glutathione S-transferase. This is the first report on such 'putatively hormetic' ageing-analogous effects of CR, besides the age-retarding ones.
authors.affiliation: Physical Biochemistry, Department of Chemistry, Technische Universität Darmstadt, Petersenstrasse 22, Darmstadt, Germany. dikshadan@gmail.com
authors.forename: Diksha
authors.initials: D
authors.lastname: Dani
authors.forename: Isao
authors.initials: I
authors.lastname: Shimokawa
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Komatsu
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
authors.forename: Uwe
authors.initials: U
authors.lastname: Warnken
authors.forename: Elham
authors.initials: E
authors.lastname: Schokraie
authors.forename: Martina
authors.initials: M
authors.lastname: Schnölzer
authors.forename: Frank
authors.initials: F
authors.lastname: Krause
authors.forename: Michiru D
authors.initials: MD
authors.lastname: Sugawa
authors.forename: Norbert A
authors.initials: NA
authors.lastname: Dencher
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D031204:Caloric Restriction; D003576:Electron Transport Complex IV / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D003576:Electron Transport Complex IV; D005978:Glutathione