[Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells].
OBJECTIVE
To construct human Egr-1 promoter luciferase reporter system and study its activity induced by ionizing radiation.


METHODS
Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay.


RESULTS
The luciferase reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48 h after IR.


CONCLUSIONS
The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation. mediated gene therapy.
Xu
Xiao-jie
XJ
Beijing Institute of Biotechnology, Beijing 100850, China.
Ding
Li-hua
LH
Wang
Ling-xue
LX
Qin
Xi
X
Cheng
Long
L
Jiang
Kai
K
Ye
Qi-nong
QN
D001483:Base Sequence; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D051766:Early Growth Response Protein 1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D017930:Genes, Reporter / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011839:Radiation, Ionizing; D013997:Time Factors; D014162:Transfection
D051766:Early Growth Response Protein 1; D008156:Luciferases