title: Novel monoclonal antibodies against Menangle virus nucleocapsid protein.
abstract: Menangle virus (MenV) is a member of the family Paramyxoviridae isolated in Australia that causes a reproductive disease of pigs. There is a need for specific immunoassays for virus detection to facilitate the diagnosis of MenV infection. Three novel monoclonal antibodies (MAbs) of the IgG1 subtype were generated by immunizing mice with recombinant yeast-expressed MenV nucleocapsid (N) protein self-assembled to nucleocapsid-like structures. One MAb was cross-reactive with recombinant N protein of Tioman virus. The epitopes of MAbs were mapped using a series of truncated MenV N proteins lacking the 29-119 carboxy-terminal amino acid (aa) residues. The epitopes of two MAbs were mapped to aa 430-460 of the MenV N protein, whilst the epitope of one MAb was mapped to residues 460-490. All three MAbs specifically recognized MenV, as indicated by immunohistochemical staining of brain tissue isolated from a field case (a stillborn piglet) of MenV infection. The MAbs against MenV N protein may be a useful tool for immunohistological diagnosis of MenV infection.
authors.affiliation: Institute of Biotechnology, V. Graiciuno 8, 02241 Vilnius Lithuania. azvirb@ibt.lt
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zvirbliene
authors.forename: Indre
authors.initials: I
authors.lastname: Kucinskaite-Kodze
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Juozapaitis
authors.forename: Rita
authors.initials: R
authors.lastname: Lasickiene
authors.forename: Dalia
authors.initials: D
authors.lastname: Gritenaite
authors.forename: Gail
authors.initials: G
authors.lastname: Russell
authors.forename: John
authors.initials: J
authors.lastname: Bingham
authors.forename: Wojtek P
authors.initials: WP
authors.lastname: Michalski
authors.forename: Kestutis
authors.initials: K
authors.lastname: Sasnauskas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D018604:Epitope Mapping; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D018098:Paramyxovirinae / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D019590:Nucleocapsid Proteins