Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis (2-DE).
Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).
Bugla-Płoskońska
Gabriela
G
Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland. gabi@microb.uni.wroc.pl
Futoma-Kołoch
Bozena
B
Skwara
Aleksandra
A
Doroszkiewicz
Włodzimierz
W
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents