Omental fat receptor interacting protein 140 mRNA expression in women with polycystic ovary syndrome.
BACKGROUND
Receptor interacting protein 140 (RIP140) is a co-repressor and essential for oocytes released by the ovary during ovulation. The aim of the study is to investigate adipose mRNA expression of RIP140 in women with polycystic ovary syndrome (PCOS) before and 3 months after laparoscopic ovarian electrocauterization (LOE).


METHODS
Adipose tissues obtained from 15 women with PCOS before and after LOE were analyzed. Ten lean, age- and BMI-matched non-PCOS women served as controls. Gene expression of RIP140 was quantified by reverse transcriptase-polymerase chain reaction.


RESULTS
The overall spontaneous ovulation rate was 73.3% in PCOS after the LOE procedure. However, no difference was found in the expression of RIP140 between women with PCOS before LOE and controls or between PCOS before and after LOE. No difference was found in RIP140 expression between obese and lean women with PCOS, or between obese PCOS and lean controls. Further, there was no correlation between RIP140 and fasting or 2-hour glucose or insulin, homeostasis model insulin resistance index (HOMA(IR)), and fasting glucose-to-insulin ratio (G(0)/I(0)) in women with PCOS.


CONCLUSIONS
RIP140 gene does not play any pivotal role in the process of ovulation, insulin resistance or fat accumulation in women with PCOS.
Seow
Kok-Min
KM
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan.
Tsai
Yieh-Loong
YL
Juan
Chi-Chang
CC
Huang
Lee-Wen
LW
Hwang
Jiann-Loung
JL
Ho
Low-Tone
LT
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism / Q000502:physiology; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D004958:Estradiol / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000097:blood; D010535:Laparoscopy; D007986:Luteinizing Hormone / Q000097:blood; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D000078723:Nuclear Receptor Interacting Protein 1; D010060:Ovulation / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000097:blood
D048868:Adaptor Proteins, Signal Transducing; D001786:Blood Glucose; D007328:Insulin; C000627127:NRIP1 protein, human; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone