title: [Development of a suspension array method for quantitative detection of Y. pestis].
abstract: OBJECTIVE
  To establish a platform for rapid, sensitive, specific, high-throughput, and simultaneous detection of multiple biothreat-associated agents, a suspension array-based immunoassay was developed for exploring the feasibility of directly simultaneous detection of Y. pestis in powder samples.
  
  
  METHODS
  The immunoassay using coupled fluorescent beads were employed to detect Y. pestis as a model from powder sample and the feasibility for detection in field samples was demonstrated by both blind and standard laboratory trials.
  
  
  RESULTS
  The newly developed suspension array appeared to be specific and sensitive, with the detection sensitivities of 0.154 ng/ml for Y. pestis F1 antigen, and the dynamic ranges of 0.154 - 4514 ng/ml, which were higher than those of corresponding conventional ELISA tests.
  
  
  CONCLUSIONS
  The suspension array could rapidly, sensitively, specifically and quantitatively detect pathogen from powder samples, which would be useful for early identification, rapid diagnosis, and response for the attack of bioterrorism and outbreak of infectious disease.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Wangjing0115@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Linjun
authors.initials: L
authors.lastname: Shan
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Kongxin
authors.initials: K
authors.lastname: Hu
authors.forename: Yousong
authors.initials: Y
authors.lastname: Hou
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ding
mesh_terms: D054045:Biological Warfare Agents; D007118:Immunoassay / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D054045:Biological Warfare Agents