[Shortcomings of traditional method for determining antibody affinity and their correction].
It was shown that application of the traditional method for antibody affinity determination may give not exact and non complete information about the characteristics of studied antibodies especially if studying specimens contain two or more species of antibodies with different affinity. In order to avoid this problem it is recommended to use another approach, suggested by us earlier. Our approach allows elucidating that studying specimens contain more than one kind of antibodies and this allows applying a proper method for an- tibody affinity evaluation. Such method, which allows determining the affinity of two antibodies in a mixture that have different affinity, is also described.
Bobrovnik
S A
SA
Demchenko
M A
MA
Komisarenko
S V
SV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D001667:Binding, Competitive; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018448:Models, Immunological; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D012710:Serum Albumin, Bovine; D010047:Ovalbumin