title: Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1.
abstract: ASF/SF2, a member of the serine-arginine (SR) protein family, has two RRM domains (RRM1 and RRM2) and a C-terminal domain rich in RS dipeptides. SR protein kinase 1 (SRPK1) phosphorylates approximately 12 of these serines using a semiprocessive mechanism. The X-ray structure of the ASF/SF2-SRPK1 complex revealed several features of the complex that raised intriguing questions about how the substrate is phosphorylated by the kinase. The part of the RS domain destined to be phosphorylated at later stages of the reaction docks to a kinase groove distal to the active site while the neighboring RRM2 binds near the active site [Ngo, J. C., et al. (2008) Mol. Cell 29, 563-576]. In this study, we investigate the interplay between the RS domain and RRM2 for stable association and phosphorylation of ASF/SF2. Despite several contacts in the enzyme-substrate complex, free RRM2 does not bind efficiently to SRPK1 unless the docking groove is occupied by the RS domain. This domain cross-talk enhances the processive phosphorylation of the RS domain. The RRM-SRPK1 contact residues control the folding of a critical beta-strand in RRM2. Unfolding of this structural element may force the N-terminal serines of the RS domain into the active site for sequential phosphorylation. Thus, ASF/SF2 represents a new class of substrates that use unique primary sequence to induce allosteric binding, processive phosphorylation, and product release.
authors.affiliation: Department of Chemistry and Biochemistry, University of California at San Diego,9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Nhat
authors.initials: N
authors.lastname: Huynh
authors.forename: Chen-Ting
authors.initials: CT
authors.lastname: Ma
authors.forename: Ngoc
authors.initials: N
authors.lastname: Giang
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hagopian
authors.forename: Jacky
authors.initials: J
authors.lastname: Ngo
authors.forename: Joseph
authors.initials: J
authors.lastname: Adams
authors.forename: Gourisankar
authors.initials: G
authors.lastname: Ghosh
mesh_terms: D000465:Algorithms; D000494:Allosteric Regulation; D000495:Allosteric Site; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics; D017510:Protein Folding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases