Comparison of clinical features and immunological parameters of patients with dehydrating diarrhoea infected with Inaba or Ogawa serotypes of Vibrio cholerae O1.
Vibrio cholerae O1, Ogawa and Inaba serotypes, both cause severe cholera. We compared clinical and immunological features in patients in Bangladesh infected with these 2 serotypes. Blood was collected from hospitalized Ogawa (N=146) or Inaba (N=191) patients at the acute stage (day 2) and 5 and 19 days later. Ogawa patients were younger than Inaba, presented with shorter duration of diarrhoea, and had more frequent abdominal pain, vomiting and need for intravenous fluids (p<0.05). Inaba patients more frequently had dark-field positive stools (p<0.01). Inaba strains were more susceptible to tetracycline and erythromycin than Ogawa strains (p<0.001). Ogawa infection produced higher plasma vibriocidal as well as IgG responses to cholera toxin B subunit, toxin-coregulated pilus subunit and lipopolysaccharide (LPS); higher IgA responses to LPS in 'antibody in lymphocyte supernatant' (ALS) specimens were also seen. These results suggest that a cholera vaccine based on the Ogawa serotype needs to be further investigated.
Khan
Ashraful I
AI
Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Chowdhury
Fahima
F
Harris
Jason B
JB
Larocque
Regina C
RC
Faruque
Abu S G
AS
Ryan
Edward T
ET
Calderwood
Stephen B
SB
Qadri
Firdausi
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000907:Antibodies, Bacterial / Q000097:blood*; D001459:Bangladesh; D002648:Child; D002675:Child, Preschool; D002771:Cholera / Q000150:complications / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D002772:Cholera Toxin / Q000276:immunology; D003681:Dehydration / Q000209:etiology* / Q000473:pathology*; D004917:Erythromycin / Q000494:pharmacology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D008875:Middle Aged; D012703:Serotyping; D013752:Tetracycline / Q000494:pharmacology; D041241:Vibrio cholerae O1 / Q000145:classification / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification*; D055815:Young Adult
D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D004917:Erythromycin; D002772:Cholera Toxin; D013752:Tetracycline