[Comparison of content of clemastanin B of Radix Isatidis in different growing areas].
OBJECTIVE
To develop an HPLC method for determination of clemastanin B which has anti-viral activity in Radix Isatidis and compare the contents of clemastanin B in the drugs from different origins.


METHODS
The samples were separated on an ZORBAX SB-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (11:89). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 225 nm. Column temperature was 30 degrees C.


RESULTS
The linear range of clemastanin B was 0.0615-1.8441 microg (r = 0.9995), the average recovery was 97.74%, RSD was 1.4% (n=9). The contents of clemastanin B were in the range of 0.269-0.900 mg x g(-1) in Radix Isatidis from different origins.


CONCLUSIONS
The method for quantitation of clemastanin B in Radix Isatidis was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis.
An
Yiqiang
Y
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Key Laboratory of Scientfic Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. anyhe2006@yahoo.com.cn
Jia
Xiaobin
X
Chang
Lili
L
Shi
Feng
F
D002681:China; D005663:Furans / Q000032:analysis*; D031219:Isatis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
D005663:Furans; D010936:Plant Extracts; C507644:clemastanin B