Dimethylsulfoxide-soluble smoking particles and nicotine affect vascular contractibility.
The aim is to study the effect of dimethylsulfoxide-soluble smoking particles (DSP) and nicotine on the contractility of rat mesenteric artery. The superior mesenteric artery segments were cultured with DSP or nicotine for 24 h. The vascular contractibility was recorded with myograph system. DSP 0.4 mL/L and nicotine 0.48 and 0.96 mg/L shifted the concentration-contractile curves induced by sarafotoxin 6c, a selective agonist for ET(B) receptor toward the left with increased E(max). DSP 0.4 mL/L and nicotine 0.96 mg/L shifted ET(A) receptor-mediated the concentration-contractile curves toward the left with increased E(max). However, nicotine 0.06 mg/L which is the equivalent concentration of nicotine in DSP 0.4 mL/L did not affect the curves and the E(max) mediated with ET(A) receptor and ET(B) receptor. DSP 0.2 and 0.4 mL/L shifted the concentration-contractile curves induced by noradrenaline toward the right with decreased E(max). Neither did nicotine 0.06 and 0.96 mg/L. Both DSP and nicotine shifted the concentration-contractile curves induced by 5-hydroxytryptamine (5-HT) toward the right parallely. DSP changed the phenotypes towards an increased efficacy of ET(A) receptor and ET(B) receptor, and a reduced efficacy of 5-HT receptor and alpha-adrenocceptor. The effects of DSP on ET(B) receptor, ET(A) receptor and alpha-adrenocceptor were independent of nicotine. The effect on 5-HT receptor was responsible to nicotine.
Zhang
Jin-Yan
JY
Department of Pharmacology, Xi'an Jiaotong University College of Medicine, Ministry of Education, Xi'an, Shaanxi, China.
Cao
Lei
L
Zheng
Xiao-Hui
XH
Xu
Cang-Bao
CB
Cao
Yong-Xiao
YX
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D017538:Mesenteric Artery, Superior / Q000187:drug effects* / Q000378:metabolism; D009538:Nicotine / Q000633:toxicity*; D009638:Norepinephrine / Q000494:pharmacology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D044022:Receptor, Endothelin A / Q000819:agonists; D044023:Receptor, Endothelin B / Q000819:agonists; D012701:Serotonin / Q000494:pharmacology; D012995:Solubility; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014661:Vasoconstriction / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects*; D014757:Viper Venoms / Q000494:pharmacology
D019332:Endothelin-1; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D014028:Tobacco Smoke Pollution; D014757:Viper Venoms; C057354:sarafotoxins s6; D012701:Serotonin; D009538:Nicotine; D009638:Norepinephrine; D004121:Dimethyl Sulfoxide