title: A ubiquitin and ubiquitin-like protein spectral library.
abstract: We have developed and validated a ubiquitin (Ub) and ubiquitin-like protein (Ubl) spectral library, consisting of 467 consensus spectra (320 unique peptides derived from autophagy-related protein 8, F-adjacent transcript 10, interferon-stimulated gene 15 kDa protein, neural precursor cell expressed developmentally down-regulated protein 8, small ubiquitin-related modifiers 1-3 and Ub, and nine of the most commonly observed Ub/Ubl chain linkages). The use of the Ub/Ubl library with a spectral matching tool (SpectraST) yields improved performance over database search engines, and can successfully identify many types of Ub/Ubl chain-derived peptides that cannot be identified by standard database search algorithms.
authors.affiliation: Ontario Cancer Institute and McLaughlin Centre for Molecular Medicine, Toronto, ON M5G 1L7 Canada.
authors.forename: Tharan
authors.initials: T
authors.lastname: Srikumar
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Jeram
authors.forename: Henry
authors.initials: H
authors.lastname: Lam
authors.forename: Brian
authors.initials: B
authors.lastname: Raught
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D030562:Databases, Protein; D006801:Humans; D008969:Molecular Sequence Data; D057386:Search Engine; D016415:Sequence Alignment; D025801:Ubiquitin / Q000737:chemistry*; D014452:Ubiquitins / Q000737:chemistry*
chemical_list: D025801:Ubiquitin; D014452:Ubiquitins