title: Novel VIM metallo-beta-lactamase variant from clinical isolates of Enterobacteriaceae from Algeria.
abstract: Five different strains of bacteria belonging to the family Enterobacteriaceae were isolated from two patients hospitalized in the intensive care unit of the Central Military Hospital of Algiers, Algeria. All five strains, one Providencia stuartii strain, two Escherichia coli strains, and two Klebsiella pneumoniae strains, were intermediate or resistant to all beta-lactams, including carbapenems. Synergy between imipenem and EDTA was observed for all five strains. The results of the PCR experiment confirmed the presence of a bla(VIM) gene in all five strains. The bla(VIM) genes were located as part of a class 1 integron on a 180-kb conjugative plasmid. They encoded a novel metallo-beta-lactamase designated VIM-19, which differed from the parental enzyme VIM-1 by only two substitutions: Ser228Arg, previously observed in the closely related enzyme VIM-4, and Asn215Lys, not previously observed in other VIM-type carbapenemases. VIM-19 was further characterized after purification through determination of its kinetic constants. This enzyme was inhibited by EDTA and hydrolyzed penicillins, cephalosporins, and carbapenems, as observed for other VIM-type carbapenemases but with greater catalytic efficiency against penicillins than VIM-1. VIM-19 is the first carbapenemase enzyme identified from an isolate from Algeria. These results confirm the emergence of VIM-4-like enzymes in members of the family Enterobacteriaceae from Mediterranean countries.
authors.affiliation: Laboratoire de Bactériologie Clinique, Centre de Biologie, CHU de Clermont-Ferrand, 58, rue Montalembert, 63003 Clermont-Ferrand, France. frobin@chu-clermontferrand.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Robin
authors.forename: Nadjet
authors.initials: N
authors.lastname: Aggoune-Khinache
authors.forename: Julien
authors.initials: J
authors.lastname: Delmas
authors.forename: Malek
authors.initials: M
authors.lastname: Naim
authors.forename: Richard
authors.initials: R
authors.lastname: Bonnet
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015780:Carbapenems / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D016680:Genome, Bacterial; D006772:Hospitals, Military; D015378:Imipenem / Q000494:pharmacology; D007525:Isoelectric Focusing; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010406:Penicillins / Q000378:metabolism; D010641:Phenotype; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D010406:Penicillins; D047090:beta-Lactams; D015378:Imipenem; D001618:beta-Lactamases