[Identification and analysis of an actinomycete strain suppressing Clavibacter michiganensis subsp, michiganensis].
OBJECTIVE
To identify and analyze bioactive compounds of an actinomycete strain Z-L-22 suppressing Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato.


METHODS
Morphological, biological and biochemical characterization, chemotaxonomy analysis and 16S rDNA sequences homology analysis were performed to identify the strain Z-L-22. Bioactive compounds were separated and retrieved by thin layer chromatography. Paper chromatography and confirmation tests were used to identify the antibiotic. PCR was carried out using the primers targeted to synthetase of the antibiotic.


RESULTS
Strain Z-L-22 belonged to Streptomyces sp. and was similar to Streptomyces setonii. Two main bioactive components were isolated by thin layer chromatography, which were all identified as actinomycin. New actinomycin synthetase gene was cloned using the primers designed from actinomycin synthetase conserve domain.


CONCLUSIONS
Strain Z-L-22 was classified as Streptomyces setonii. Actinomycin produced by Streptomyces setonii was first reported.
Zhang
Yan
Y
College of Plant Protection, Agricultural University of Hebei, Biological Control Center for Plant Disease and Plant Pests of Hebei Province, Baoding 071001, China. geneclone@126.com
Zhang
Weihong
W
Wang
Songhong
S
Li
Yaning
Y
Zhao
Zhiquan
Z
Liu
Daqun
D
Yang
Wenxiang
W
D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003609:Dactinomycin / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology*; D008835:Micrococcaceae / Q000502:physiology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*
D001426:Bacterial Proteins; D003609:Dactinomycin