PEG enhances viral clearance on ceramic hydroxyapatite.
Viral clearance across ceramic hydroxyapatite (CHT) was examined in two elution systems: sodium chloride and sodium chloride plus poly(ethylene glycol) (PEG). In both cases clearance of xenotropic murine leukemia virus was significant (3-4 log) while that of minute virus of mice varied between 1.7 and 2.7 log; in addition, the addition of PEG to the elution buffer enhanced viral clearance. The data are in agreement with the previous results and demonstrate that additional clearance can be obtained by adding PEG to a ceramic hydroxyapatite buffer system.
Snyder
Mark A
MA
Bio-Rad Laboratories, Hercules, CA, USA. mark_snyder@bio-rad.com
Ng
Paul
P
Mekosh
Heather
H
Gagnon
Pete
P
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002516:Ceramics; D002845:Chromatography / Q000379:methods; D017886:Durapatite; D009052:Leukemia Virus, Murine / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008915:Minute Virus of Mice / Q000276:immunology / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D014780:Viruses / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017886:Durapatite