title: Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells.
abstract: The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40 degrees C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.
authors.affiliation: Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Wei
authors.forename: Ryohei
authors.initials: R
authors.lastname: Ogawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takasaki
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Zhao
authors.forename: Hua-Chuan
authors.initials: HC
authors.lastname: Zheng
authors.forename: Kanwal
authors.initials: K
authors.lastname: Ahmed
authors.forename: Mariame A
authors.initials: MA
authors.lastname: Hassan
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D003911:Dextrans / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D045325:HCT116 Cells; D006367:HeLa Cells; D018869:Heat-Shock Response; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods*
chemical_list: D003911:Dextrans; D012333:RNA, Messenger; C015219:fluorescein isothiocyanate dextran; D008156:Luciferases; D016650:Fluorescein-5-isothiocyanate