[Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain].
OBJECTIVE
To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine.


METHODS
By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter.


RESULTS
Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer.


CONCLUSIONS
The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.
Guo
Li
L
Graduate School, Jilin Agriculture University, Changchun 130118, China. piaogl110@163.com
Feng
Na
N
Yang
Songtao
S
Wang
Xijun
X
Ge
Jinying
J
Xia
Xianzhu
X
Bu
Zhigao
Z
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D005821:Genetic Techniques; D011818:Rabies / Q000276:immunology / Q000821:virology*; D011819:Rabies Vaccines / Q000235:genetics* / Q000276:immunology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
D011819:Rabies Vaccines; D014764:Viral Proteins