Role of hydroperoxide lyase in white-backed planthopper (Sogatella furcifera Horváth)-induced resistance to bacterial blight in rice, Oryza sativa L.
A pre-infestation of the white-backed planthopper (WBPH), Sogatella furcifera Horváth, conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice (Oryza sativa L.) under both laboratory and field conditions. The infestation of another planthopper species, the brown planthopper (BPH) Nilaparvata lugens Stål, did not significantly reduce the incidence of bacterial blight symptoms. A large-scale screening using a rice DNA microarray and quantitative RT-PCR revealed that WBPH infestation caused the upregulation of more defence-related genes than did BPH infestation. Hydroperoxide lyase 2 (OsHPL2), an enzyme for producing C(6) volatiles, was upregulated by WBPH infestation, but not by BPH infestation. One C(6) volatile, (E)-2-hexenal, accumulated in rice after WBPH infestation, but not after BPH infestation. A direct application of (E)-2-hexenal to a liquid culture of Xoo inhibited the growth of the bacterium. Furthermore, a vapour treatment of rice plants with (E)-2-hexenal induced resistance to bacterial blight. OsHPL2-overexpressing transgenic rice plants exhibited increased resistance to bacterial blight. Based on these data, we conclude that OsHPL2 and its derived (E)-2-hexenal play some role in WBPH-induced resistance in rice.
Gomi
Kenji
K
National Institute of Agrobiological Sciences, NIAS, Tsukuba, Ibaraki 305-8602, Japan.
Satoh
Masaru
M
Ozawa
Rika
R
Shinonaga
Yumi
Y
Sanada
Sachiyo
S
Sasaki
Katsutomo
K
Matsumura
Masaya
M
Ohashi
Yuko
Y
Kanno
Hiroo
H
Akimitsu
Kazuya
K
Takabayashi
Junji
J
D000446:Aldehyde-Lyases / Q000235:genetics / Q000502:physiology*; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D006430:Hemiptera / Q000502:physiology*; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000469:parasitology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014974:Xanthomonas / Q000472:pathogenicity*
D000447:Aldehydes; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C010463:n-hexanal; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase