[Study on DNA damage in L-02 cell induced by acrylamide].
OBJECTIVE
To investigate the cell cycle, cell apoptosis and DNA damage induced by acrylamide (AA) in L-02 human liver embryo cell line.


METHODS
The rate of cell survivors, cell cycle, cell apoptosis and DNA damage were measured respectively after L-02 cell were exposed to 0.01, 0.1, 0.5, 1.0, 2.5, 5.0 and 7.5 mmol/L AA for 12h and 24h in vitro.


RESULTS
The rates of cellular survivors in the low (0.01 mmol/L and 0.1 mmol/) dose AA-treated group were higher than those in control group, but those in the middle (0.5, 1.0 and 2.5 mmol/L) and high (5.0 mmol/L and 7.5 mmol/L) dose AA-treated groups were significantly lower than those in control group (P < 0.05). The rate of cell in G1 stage was reduced along with the promotion of AA, and the middle and high (> 1.0 mmol/L) dose AA-treated groups were significantly lower than the control group (P < 0.05), while the rate of cell in S stage was in reverse. The percentages of apoptosis were significantly higher than that of the control group in the high dose AA-treated groups (P < 0.05). The percents of tail DNA and Oliver Tail Moment (OTM) were significantly higher than that of the control group in the middle and high (> 1.0 mmol/L) dose AA-treated groups (P < 0.05).


CONCLUSIONS
AA could induce the confusion and apoptosis of L-02 cell, and has impairment effect on DNA.
Xu
Jian
J
Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China.
Wang
Rui
R
Liu
Jianjun
J
Qian
Qifeng
Q
Li
Qing
Q
Liu
Xiaoli
X
D020106:Acrylamide / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans
D020106:Acrylamide