title: Resolution of adiponectin oligomers in human plasma using free flow electrophoresis.
abstract: Adiponectin is an important adipocytokine hormone which circulates in blood as homo-oligomers (trimer, hexamer and high molecular weight (HMW) forms) as well as a truncated form corresponding to the globular domain. Free flow electrophoresis (FFE) used in zone electrophoresis mode revealed the presence of isoforms within these oligomeric forms in plasma. HMW adiponectin oligomer showed two isoforms which carry different charge density at pH 4.7, only one of which is susceptible to dissociation by SDS. The adiponectin hexamer was shown to consist of a doublet and also shown to have at least two isoforms. A truncated form of adiponectin was identified as the main constituent of adiponectin in plasma and appeared to circulate bound to a basic protein, potentially one of the chemokines reported to bind to the globular domain. Analysis of the monomer composition of the oligomers revealed differences in monomeric isoforms used to build up the oligomers.
authors.affiliation: BD Diagnostics, Am Klopferspitz 19a, Martinsried, Germany.
authors.forename: Aude L
authors.initials: AL
authors.lastname: Foucher
authors.forename: Kristina
authors.initials: K
authors.lastname: Hartmann
authors.forename: Monika
authors.initials: M
authors.lastname: Hauptmann
authors.forename: Robert
authors.initials: R
authors.lastname: Wildgruber
authors.forename: Michael
authors.initials: M
authors.lastname: Safinowski
authors.forename: Thomas
authors.initials: T
authors.lastname: Forst
authors.forename: Andreas
authors.initials: A
authors.lastname: Pfützner
authors.forename: Craig A
authors.initials: CA
authors.lastname: Gelfand
authors.forename: Mikkel
authors.initials: M
authors.lastname: Nissum
mesh_terms: D052242:Adiponectin / Q000097:blood*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D020033:Protein Isoforms / Q000737:chemistry; D055503:Protein Multimerization
chemical_list: D052242:Adiponectin; D020033:Protein Isoforms