title: Paenibacillus prosopidis sp. nov., isolated from the nodules of Prosopis farcta.
abstract: A bacterial strain, designated PW21(T), was isolated from root nodules of Prosopis farcta in Tunisia. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate into the genus Paenibacillus, with its closest relatives being Paenibacillus glycanilyticus DS-1(T) and Paenibacillus castaneae Ch-32(T) with identity values of 96.9 %. DNA-DNA hybridization measurements showed values of less than 25 % with respect to these two species. The isolate was a Gram-variable, motile and sporulating rod. Catalase activity was positive and oxidase activity was weakly positive. Aesculin, CM-cellulose, xylan and starch were hydrolysed but casein and gelatin were not. Acetoin production was weakly positive and nitrate reduction was negative. Urease production was negative. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the predominant menaquinone and anteiso-C(15 : 0), iso-C(16 : 0) and iso-C(15 : 0) were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, a glycolipid, six phospholipids, an unidentified lipid and two unknown aminophosphoglycolipids. meso-Diaminopimelic acid was not detected in the peptidoglycan. The DNA G+C content of the isolate was 52.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain PW21(T) should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus prosopidis sp. nov. is proposed. The type strain is PW21(T) (=LMG 25259(T) =CECT 7506(T) =DSM 22405(T)).
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Valverde
authors.affiliation: Laboratoire de Biotechnologies Végétales Appliquées a l'Amélioration des cultures, Faculté des Sciences de Gabes, Gabes, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: Fterich
authors.affiliation: Laboratoire de Biotechnologies Végétales Appliquées a l'Amélioration des cultures, Faculté des Sciences de Gabes, Gabes, Tunisia.
authors.forename: Mosbah
authors.initials: M
authors.lastname: Mahdhi
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Sevilla, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Caviedes
authors.affiliation: Laboratoire de Biotechnologies Végétales Appliquées a l'Amélioration des cultures, Faculté des Sciences de Gabes, Gabes, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mars
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Sevilla, Spain.
authors.forename: Ignacio D
authors.initials: ID
authors.lastname: Rodriguez-Llorente
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D029862:Prosopis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S