title: Molecular epidemiological and serological studies of hantavirus infection in northern Vietnam.
abstract: The distribution of anti-hantavirus antibodies in humans and rodents in northern Vietnam was examined. In total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for IgG antibodies against Hantaan or Seoul virus (SEOV) by ELISA, IFA, and Western blot analysis. Antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of Hai Phong, and 2/185 residents of Ha Nam Province. In comparison, positive sera were detected in 5/220 (2.3%) fever patients in the provinces of Ha Nam (1/58) and Thanh Hoa (4/146). Antibody-positive Rattus norvegicus were found in the provinces of Ha Nam (7/52) and Thanh Hoa (1/67), in Haibatrung District (7/43) in Hanoi, and in Hai Phong Port (21/62), while antibody-positive R. rattus (2/17) were found in Hai Phong Port. Part of the Gc region from the viral genome was amplified by RT-PCR using lung tissue samples from R. norvegicus in Haibatrung (2/7) and Hai Phong Port (7/9), but not from R. rattus (0/2). Viral sequences were located in the SEOV clade and formed a single lineage with Indonesian SEOV, suggesting that Vietnamese SEOV is part of a distinct lineage among Asian SEOVs.
authors.affiliation: National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
authors.forename: Thua Thang
authors.initials: TT
authors.lastname: Truong
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Koichi
authors.initials: K
authors.lastname: Araki
authors.forename: Byoung-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakamura
authors.forename: Rika
authors.initials: R
authors.lastname: Endo
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimizu
authors.forename: Shumpei P
authors.initials: SP
authors.lastname: Yasuda
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koma
authors.forename: Midori
authors.initials: M
authors.lastname: Taruishi
authors.forename: Megumi
authors.initials: M
authors.lastname: Okumura
authors.forename: Uyen Ninh
authors.initials: UN
authors.lastname: Truong
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006476:Orthohantavirus / Q000235:genetics*; D018778:Hantavirus Infections / Q000097:blood* / Q000453:epidemiology*; D006801:Humans; D010802:Phylogeny; D012376:Rodent Diseases / Q000097:blood; D012377:Rodentia; D014744:Vietnam / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral