[Protective effects of catalpol against H2O2-induced oxidative damage in astrocytes].
OBJECTIVE
To study the protective effects of catalpol against H2O2-induced oxidative damage in astrocytes.


METHODS
Astrocytes were pretreated with 1, 10, 100 micromol x L(-1) catalpol and then exposed to H2O2. Effects of catalpol on cell morphous were observed by inverted microscope. Cell viability were detected by MTT assay. LDH content in the cell culture fluid, and intracellular T-SOD, GSH-Px activity and MDA content were assessed by colorimetric method.


RESULTS
The results showed that catalpol evidently ameliorated cell morphous, improved cell viability, and maintained integrality of cell membrane. Furthermore, catalpol could significantly inhibit the decrease of intracellullar T-SOD and GSH-Px activity, and the increase of MDA content.


CONCLUSIONS
Catalpol have protective effects against H2O2-induced oxidative damage in astrocytes.
Zhang
Ziqiang
Z
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. zzq828929@163.com
Liu
Yumei
Y
Xue
Bangqun
B
Wei
Lan
L
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D020011:Protective Agents; C078040:catalpol; D006861:Hydrogen Peroxide