Slow release of nitric oxide from charged catheters and its effect on biofilm formation by Escherichia coli.
Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. The catheters demonstrated slow release of nitric oxide over a 14-day period. The charged catheters were rendered antiseptic, and as such, were able to prevent bacterial colonization and biofilm formation on their luminal and exterior surfaces. In addition, we observed that NO-impregnated catheters were able to inhibit the growth of Escherichia coli within the surrounding media, demonstrating the ability to eradicate a bacterial concentration of up to 10(4) CFU/ml.
Regev-Shoshani
Gilly
G
Department of Medicine, Division of Infectious Diseases, University of British Columbia, 2733 Heather St., Vancouver, BC, Canada V5Z 3J5.
Ko
Mary
M
Miller
Chris
C
Av-Gay
Yossef
Y
D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002404:Catheterization; D015169:Colony Count, Microbial; D003692:Delayed-Action Preparations; D004356:Drug Storage; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology*; D014546:Urinary Catheterization; D014563:Urodynamics / Q000502:physiology
D003692:Delayed-Action Preparations; D009569:Nitric Oxide