[Study on fingerprint of Maodongqing injection by HPLC].
OBJECTIVE
To establish the fingerprint of Maodongqing injection by HPLC.


METHODS
The column of ODS-2 Hypersil C18 (4.6 mm x 200 mm, 5 microm) was used. The mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution. The flow rate was 1 mL/min, the column temperature was 30 degrees C, and the detection wavelength was 325 nm.


RESULTS
The fingerprint of Maodongqing injection was established, and 8 samples of different batches of Maodongqing injection were detected. Thirteen peaks in the chromatogram were marked by "Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A)". There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of the fingerprints of Chinese traditional medicine.


CONCLUSIONS
This method is accurate, reliable and provides a scientific basis for controlling the quality of Maodongqing injection.
Lai
Shan
S
Guangdong Institute for Drug Control, Guangzhou 510180, China. laishan_77@163.com
Yang
Li-Wei
LW
Ou
Guo-Deng
GD
Li
Yong-Xue
YX
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D030017:Ilex / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal