Comparative in vitro cytotoxicity toward human osteoprogenitor cells of polycaprolactones synthesized from various metallic initiators.
Various poly(epsilon-caprolactone)s (PCLs) prepared by ring-opening polymerization of epsilon-caprolactone (CL) initiated by a range of metallic derivatives such as alkoxides, Al(OiPr)(3), La(OiPr)(3), Sn(octanoate)(2), Ti(OiPr)(4), Zn[O(CH(2))(3)NH(t)Boc](2), or borohydride La(BH(4))(3)(THF)(3) were evaluated for their in vitro cytotoxicity. The amount of residual metal present in the polymer samples was measured and compared to the initial quantity introduced. The effect of the metallic system on the biocompatibility profiles of the resulting polyesters was evaluated in vitro on PCL films from a series of cytotoxicity tests involving MTT and neutral red assays upon exposure to human osteoprogenitor cells. The absence of toxic influence of all these PCLs suggests that they may be used as biomaterials in contact with living human cells.
Schappacher
Michèle
M
Laboratoire de Chimie des Polymères Organiques, LCPO, Université Bordeaux 1- CNRS - ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac Cedex, France.
Le Hellaye
Maude
M
Bareille
Reine
R
Durrieu
Marie-Christine
MC
Guillaume
Sophie M
SM
D001842:Bone and Bones / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D008670:Metals / Q000737:chemistry; D011091:Polyesters / Q000138:chemical synthesis* / Q000633:toxicity*
D008670:Metals; D011091:Polyesters; C016240:polycaprolactone