title: [Antifungal sctivities and stability of extracts from culture liquid of Hypoxylon perforatum to Sphaeropsis sapinea].
abstract: OBJECTIVE
  Our research objective is to obtain the active substances from culture liquid of Hypoxylon perforatum with inhibitory effect on Sphaeropsis sapinea growth and germination.
  
  
  METHODS
  Water and ester were used for extracting active substances from culture liquid of Hypoxylon perforatum, either by extracting directly from cultural liquid, or extracting under ultrasonic. Growth-inhibiting rate and germination-inhibiting rate were used as the index of antifungal activity.
  
  
  RESULTS
  Five extracts from culture liquid of Hypoxylon perforatum showed antifungal activities to Sphaeropsis sapinea growth and germination.
  
  
  CONCLUSIONS
  Ethyl acetate-extract from culture liquid of Hypoxylon perforatum has a highly antifungal activity to Sphaeropsis sapinea, high stability in the natural environment. So it has a highly development of the value and application prospect. The mainly inhibiting active substance is methyl p-methoxy cinnamate, which as medicine intermediate was used in cosmetic to be UV-protection absorbefacient. Diisobutyl phthalate and Dibutyl phthalate are all the common plastic-enhancer for PolyvinylChloride (PVC). It is very important in medicinal industry and chemistry industry to discovering the natural presence of the three compounds.
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China. songrq1964@163.com
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Song
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gao
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D001685:Biological Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D013098:Xylariales / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D001685:Biological Factors; D003470:Culture Media