Transepithelial transport of macromolecular substances in IL-4 treated human intestinal T84 cell monolayers.
The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 degrees C. The permeability of T84 cell monolayers to beta-lactogulobulin (beta-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN(3)), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4.
Mochizuki
Tetsunosuke
T
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. atmochi@mail.ecc.u-tokyo.ac.jp
Satsu
Hideo
H
Totsuka
Mamoru
M
Shimizu
Makoto
M
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D045744:Cell Line, Tumor; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism; D007782:Lactoglobulins / Q000378:metabolism; D046911:Macromolecular Substances / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D019810:Sodium Azide / Q000494:pharmacology; D013696:Temperature
D003911:Dextrans; D007546:Isoquinolines; D007782:Lactoglobulins; D046911:Macromolecular Substances; C015219:fluorescein isothiocyanate dextran; D015847:Interleukin-4; D000255:Adenosine Triphosphate; D010047:Ovalbumin; C017475:lucifer yellow; D019810:Sodium Azide; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate