Evaluation of active and passive transport processes in corneas extracted from preserved rabbit eyes.
In vitro transcorneal permeability studies are an important screening tool in drug development. The objective of this research is to examine the feasibility of using corneas isolated from preserved rabbit eyes as a model for permeability evaluation. Eyes from male New Zealand White rabbits were used immediately or were stored overnight in phosphate-buffered saline (PBS) or Hanks balanced salt solution (HBSS) over wet ice. Integrity of isolated corneas was evaluated by measuring the TEER and by determining the permeability of paracellular and transcellular markers. Active transport was assessed by measuring transcorneal permeability of selected amino acids. Esterase activity was estimated using p-nitrophenyl assay. In all cases, corneas from freshly enucleated eyes were compared to those isolated from the day-old preserved eyes. Transcellular and paracellular passive diffusion was not affected by the storage medium and observed to be similar in the fresh and preserved eye models. However, amino acid transporters demonstrated lower functional activity in corneas excised from eyes preserved in PBS. Moreover, preserved eyes displayed almost 1.5-fold lower esterase activity in the corneal tissue. Thus, corneas isolated from day-old eyes, preserved in HBSS, closely mimics freshly excised rabbit corneas in terms of both active and passive transport characteristics but possesses slightly reduced enzymatic activity.
Majumdar
Soumyajit
S
Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA. majumso@olemiss.edu
Hingorani
Tushar
T
Srirangam
Ramesh
R
D026905:Amino Acid Transport Systems / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D001693:Biological Transport, Active; D003315:Cornea / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D017097:Electric Impedance; D004950:Esterases / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry; D011817:Rabbits
D026905:Amino Acid Transport Systems; D019188:Organ Preservation Solutions; D004950:Esterases