title: Direct chemical cross-linking of platelet-derived growth factor-BB to the demineralized bone matrix improves cellularization and vascularization.
abstract: In previous studies, we have described the use of demineralized bone matrix (DBM) as a carrier for the localized delivery system of growth factors in vitro and in vivo. The aim of the present work was to develop a direct chemical approach to immobilize the platelet-derived growth factor-BB (PDGF-BB) on DBM with cross-linking reagents. The amount of PDGF-BB covalently immobilized on DBM was significantly increased. The increased proliferation of fibroblasts demonstrated that the biological activity of PDGF-BB was not significantly reduced by cross-linking. Compared with control groups, there was a statistically significant increase in blood vessel density in the PDGF-C-DBM group after having been subcutaneously implanted into the dorsal side of the rats. The surface bioactivity of scaffolds on stimulation cell and new blood vessel invasion was improved. Therefore, the direct chemical cross-linking approach could be used to retain growth factors on collagen scaffolds effectively to develop functional biomaterials.
authors.affiliation: Biotechnology Research Center/Hubei Province Key Laboratory of Natural Products Research and Development, China Three Gorges University, Yichang 443002, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sun
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Maojin
authors.initials: M
authors.lastname: Yang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Gao
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D000077214:Becaplermin; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000737:chemistry* / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
chemical_list: D003432:Cross-Linking Reagents; D055767:Immobilized Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen