STUDIES IN THE RELATION OF THE HEMOLYTIC STREPTOCOCCUS TO RHEUMATIC FEVER : II. FRACTIONATION OF THE HEMOLYTIC STREPTOCOCCUS BY HIGH SPEED CENTRIFUGATION.
AFTER DISINTEGRATION BY SONIC VIBRATIONS THE CONTENTS OF THE HEMOLYTIC STREPTOCOCCUS CAN BE SEPARATED BY DIFFERENTIAL CENTRIFUGATION INTO THREE FRACTIONS: an insoluble residue, cytoplasmic particles, and a solution of proteins of smaller unit size. The residue (R) presumably comprises the cell walls of the bacteria and contains the type-specific M protein. The cytoplasmic particles (CP) contain some lipoid, the group-specific carbohydrate, and nucleoprotein of the ribose type. The supernate fraction (S) contains two components, presumably protein, at least one of which is part of a dissociable nucleoprotein of the desoxyribose type. Both CP and S precipitate and fix complement with antistreptococcal sera. Both give rise to antibodies on injection into rabbits. Both are of broad reactivity. CP and S can be shown to be serologically distinct by several means, including cross-absorption tests. On continued disintegration of the organism an inverse relation is noted between the yield of R and that of CP, whereas the yield of S is constant. A theory as to the structure of the streptococcal cell is offered in terms of the data presented.
Harris
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The Children's Hospital of Philadelphia (Department of Pediatrics, School of Medicine, University of Pennsylvania), the Children's Seashore House for Invalid Children, Atlantic City, and the Philadelphia General Hospital.
D000818:Animals; D000906:Antibodies; D002473:Cell Wall; D002498:Centrifugation; D011506:Proteins; D011817:Rabbits; D012213:Rheumatic Fever; D013291:Streptococcus
D000906:Antibodies; D011506:Proteins