title: Bioreactor expansion of human neural precursor cells in serum-free media retains neurogenic potential.
abstract: Human neural precursor cells (hNPCs), harvested from somatic tissue and grown in vitro, may serve as a source of cells for cell replacement strategies aimed at treating neurodegenerative disorders such as Parkinson's disease (PD), Huntington's disease (HD), and intractable spinal cord pain. A crucial element in a robust clinical production method for hNPCs is a serum-free growth medium that can support the rapid expansion of cells while retaining their multipotency. Here, we report the development of a cell growth medium (PPRF-h2) for the expansion of hNPCs, achieving an overall cell-fold expansion of 10(13) over a period of 140 days in stationary culture which is significantly greater than other literature results. More importantly, hNPC expansion could be scaled-up from stationary culture to suspension bioreactors using this medium. Serial subculturing of the cells in suspension bioreactors resulted in an overall cell-fold expansion of 7.8 x 10(13) after 140 days. These expanded cells maintained their multipotency including the capacity to generate large numbers of neurons (about 60%). In view of our previous studies regarding successful transplantation of the bioreactor-expanded hNPCs in animal models of neurological disorders, these results have demonstrated that PPRF-h2 (containing dehydroepiandrosterone, basic fibroblast growth factor and human leukemia inhibitory factor) can successfully facilitate the production of large quantities of hNPCs with potential to be used in the treatment of neurodegenerative disorders.
authors.affiliation: Pharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada.
authors.forename: Behnam A
authors.initials: BA
authors.lastname: Baghbaderani
authors.forename: Karim
authors.initials: K
authors.lastname: Mukhida
authors.forename: Arindom
authors.initials: A
authors.lastname: Sen
authors.forename: Michael S
authors.initials: MS
authors.lastname: Kallos
authors.forename: Murray
authors.initials: M
authors.lastname: Hong
authors.forename: Ivar
authors.initials: I
authors.lastname: Mendez
authors.forename: Leo A
authors.initials: LA
authors.lastname: Behie
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D006801:Humans; D019636:Neurodegenerative Diseases / Q000628:therapy*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*