MicroRNAs that respond to histone deacetylase inhibitor SAHA and p53 in HCT116 human colon carcinoma cells.
MicroRNAs (miRNAs) are important post-transcriptional regulators involved in many biological processes. We investigated the expression profiles of miRNAs affected by the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), and p53 in the human colon cancer cell line, HCT116 (wt-p53) and its derivative, HCT116 (null-p53). In a microarray assay, 144 of 275 human miRNAs showed several-fold changes in transcription. Most of these miRNAs were strongly affected by SAHA, and their expression profiles varied depending on the presence of p53. Thirty-one miRNAs showing the greatest expression changes were selected for target prediction, and genes related to apoptosis (102), cell cycle (38), and differentiation (102) were predicted. Each miRNA had many target genes, and several genes also were targeted by many miRNAs. Putative p53 upstream binding sites for the miRNAs were determined, and most sites scored >85%, suggesting a high probability of binding. In conclusion, we identified several miRNAs whose expression was affected by both SAHA and p53. Many of the miRNAs showed dramatic changes and were predicted to target many mRNAs. Further studies will be needed to verify these predictions.
Shin
Sangsu
S
Functional Genoproteome Research Centre, Konkuk University, Seoul 143-701, Korea.
Lee
Eun-Mee
EM
Cha
Hwa Jun
HJ
Bae
Seunghee
S
Jung
Jin Hyuk
JH
Lee
Sun-Mi
SM
Yoon
Youngmin
Y
Lee
Hyunjin
H
Kim
Sumi
S
Kim
Hyunsook
H
Lee
Su-Jae
SJ
Park
In-Chul
IC
Jin
Young-Woo
YW
An
Sungkwan
S
D004791:Enzyme Inhibitors / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D035683:MicroRNAs / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D000077337:Vorinostat
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D000077337:Vorinostat