Effect of transplantation of peripheral blood mononuclears obtained using granulocytic colony-stimulating factor and hyaluronidase on regeneration of hemopoietic tissue during myelosuppression.
The mechanisms of hemopoiesis recovery after transplantation of peripheral blood mononuclears obtained using granulocytic CSF and granulocytic CSF in combination with hyaluronidase were studied on the model of cytostatic myelosuppression. It was found that regeneration of the hemopoietic tissue resulted from an increase in the pool of erythroid and granulomonocytic precursors and in their functional activity. The increase in the count of fibroblast precursors in the bone marrow, higher production of hemopoietins by adherent myelokaryocytes, and an increase in the level of humoral factors in the serum were detected after injection of the transplants. A higher therapeutic efficiency of cell material obtained after combined use of granulocytic CSF and hyaluronidase was shown.
Dygai
A M
AM
Institute of Pharmacology, Tomsk Research Center, Siberian Division of the Russian Academy of Medical Sciences, Russia. dygay@rambler.ru
Zyuz'kov
G N
GN
Zhdanov
V V
VV
Simanina
E V
EV
Stavrova
L A
LA
Udut
E V
EV
Khrichkova
T Yu
TY
Minakova
M Yu
MY
Ermakova
N N
NN
Firsova
T V
TV
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D017690:Cell Transplantation; D005472:Fluorouracil / Q000008:administration & dosage; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*
D016179:Granulocyte Colony-Stimulating Factor; D006821:Hyaluronoglucosaminidase; D005472:Fluorouracil