title: Structural and immunochemical studies of neutral exopolysaccharide produced by Lactobacillus johnsonii 142.
abstract: This paper describes the structure of neutral exopolysaccharide (EPS) produced by Lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (IBD). Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, NOESY, and (1)H,(13)C HSQC experiments revealed that the repeating unit of the EPS is a pentasaccharide: -->3)-alpha-D-Galp-(1-->3)-beta-d-Glcp-(1-->5)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->3)-alpha-D-Galp-(1--> The rabbit antiserum raised against whole cells of L. johnsonii 142 reacted with homologous EPS, and cross-reacted with exopolysaccharide from Lactobacillus animalis/murinus 148 isolated also from mice with IBD, but not reacted with EPS of L. johnsonii 151 from healthy mice.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Górska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Jachymek
authors.forename: Jacek
authors.initials: J
authors.lastname: Rybka
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Piotr B
authors.initials: PB
authors.lastname: Heczko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D015212:Inflammatory Bowel Diseases / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D007778:Lactobacillus / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D011135:Polysaccharides, Bacterial