A ubiquitin and ubiquitin-like protein spectral library.
We have developed and validated a ubiquitin (Ub) and ubiquitin-like protein (Ubl) spectral library, consisting of 467 consensus spectra (320 unique peptides derived from autophagy-related protein 8, F-adjacent transcript 10, interferon-stimulated gene 15 kDa protein, neural precursor cell expressed developmentally down-regulated protein 8, small ubiquitin-related modifiers 1-3 and Ub, and nine of the most commonly observed Ub/Ubl chain linkages). The use of the Ub/Ubl library with a spectral matching tool (SpectraST) yields improved performance over database search engines, and can successfully identify many types of Ub/Ubl chain-derived peptides that cannot be identified by standard database search algorithms.
Srikumar
Tharan
T
Ontario Cancer Institute and McLaughlin Centre for Molecular Medicine, Toronto, ON M5G 1L7 Canada.
Jeram
Stanley M
SM
Lam
Henry
H
Raught
Brian
B
D000465:Algorithms; D000595:Amino Acid Sequence; D030562:Databases, Protein; D006801:Humans; D008969:Molecular Sequence Data; D057386:Search Engine; D016415:Sequence Alignment; D025801:Ubiquitin / Q000737:chemistry*; D014452:Ubiquitins / Q000737:chemistry*
D025801:Ubiquitin; D014452:Ubiquitins