title: Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods.
abstract: This study surveyed tetracycline residues in foods marketed in Kuwait using various techniques to determine their prevalence above the threshold level. A total of 1517 locally produced and imported food samples of animal origin were collected for analyses, comprising dairy products, eggs and tissue samples (meat, poultry and fish) to give a representative picture of the current use and/or misuse of the drug in Kuwait. Screening was carried out using the Charm II test for tetracycline residues. Positive and suspect positive samples were confirmed by LC/MS/MS. Negative and positive controls, in triplicate, were applied to each method and showed 80-100% agreement. The results showed that 100% of tested eggs, meat, fish, ice cream and cheese were within the limit, while 5% of poultry and 18% of milk samples were above the permitted limit.
authors.affiliation: Biotechnology Department, Food Resource and Biological Science Division, Kuwait Institute for Science Research, Kuwait.
authors.forename: Hani M
authors.initials: HM
authors.lastname: Al-Mazeedi
authors.forename: Alaa B
authors.initials: AB
authors.lastname: Abbas
authors.forename: Hussam F
authors.initials: HF
authors.lastname: Alomirah
authors.forename: Wafaa Y
authors.initials: WY
authors.lastname: Al-Jouhar
authors.forename: Siham A
authors.initials: SA
authors.lastname: Al-Mufty
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Ezzelregal
authors.forename: Rashed A
authors.initials: RA
authors.lastname: Al-Owaish
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004350:Drug Residues; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007730:Kuwait; D011863:Radioimmunoassay / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013752:Tetracycline / Q000737:chemistry*
chemical_list: D013752:Tetracycline