title: Histologic changes in the auditory tube mucosa of rats after long-term exposure to cigarette smoke.
abstract: OBJECTIVE
  The aim of the study was to investigate the effect of cigarette smoke on the auditory tube and middle ear mucosa after long-term exposure (4 and 6 months).
  
  
  METHODS
  Fifteen rats were divided into 3 groups. The experimental groups were exposed to cigarette in a smoking chamber for 4 and 6 months (n = 5 each). A control group (n = 5) was placed in the same chamber without exposure to cigarette smoke. Histologic changes of the auditory tube mucosa were observed through light and electron microscopes. Histologic changes of the middle ear mucosa were also observed through light microscopes.
  
  
  RESULTS
  The histologic changes consisted of a proliferation of goblet cells and an increase of mucus secretion in auditory tube. Squamous metaplasia was paradoxically decreased according to the duration of exposure in auditory tube. The number of goblet cell was gradually increased according to the duration of exposure in the auditory tube and middle ear.
  
  
  CONCLUSIONS
  Long-term passive smoke directly affects the auditory tube and middle ear mucosa. Histologic changes of auditory tube mucosa consisted of goblet cell proliferation and excessive mucus secretion.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Pusan National University School of Medicine, Busan, Korea.
authors.forename: Soo-Keun
authors.initials: SK
authors.lastname: Kong
authors.forename: Kyong-Myong
authors.initials: KM
authors.lastname: Chon
authors.forename: Eui-Kyung
authors.initials: EK
authors.lastname: Goh
authors.forename: Il-Woo
authors.initials: IW
authors.lastname: Lee
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Soo-Geun
authors.initials: SG
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004781:Environmental Exposure / Q000009:adverse effects; D005064:Eustachian Tube / Q000473:pathology* / Q000648:ultrastructure; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008679:Metaplasia; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D009093:Mucus / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D014028:Tobacco Smoke Pollution