title: [The effect of gallic acid extracted from leaves of Phyllanthus emblica on apoptosis of human hepatocellular carcinoma BEL-7404 cells].
abstract: OBJECTIVE
  To explore the effect of gallic acid extracted from Leaves of Phyllanthus emblica on the apoptosis of BEL-7404 cells.
  
  
  METHODS
  MTT assay was applied to detect the influence on prolifetation in vitro. Inverted microscope was utilized to observe the morphological changes after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method was used to detect earlier period apoptosis cells and Tunel was applied to calculate the apoptosis rates.
  
  
  RESULTS
  Gallic acid could restrain the BEL-7404 cells proliferation at diffierent levels in a time and concentration dependent manner. The typical morphological changes of apoptosis were observed after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method and Tunel method showed that the viable apoptotic cell and apoptosis rates added as action time prolonged.
  
  
  CONCLUSIONS
  Gallic acid can restrain the BEL-7404 cells proliferation and induce apoptosis, and its effect on apoptosis is time dependent.
authors.affiliation: Guangxi Traditional Chinese Medical University, Nanning 530001, China. zhongzg@gxtcmu.edu.cn
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Zhong
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Zhong
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Zhang
authors.forename: Deng-Pan
authors.initials: DP
authors.lastname: Wu
authors.forename: Chun-Lan
authors.initials: CL
authors.lastname: Zeng
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Wang
authors.forename: Yong-Han
authors.initials: YH
authors.lastname: Wei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D029842:Phyllanthus emblica / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013997:Time Factors
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005707:Gallic Acid