title: An improved facile method for extraction and determination of steroidal saponins in Tribulus terrestris by focused microwave-assisted extraction coupled with GC-MS.
abstract: An improved fast method for extraction of steroidal saponins in Tribulus terrestris based on the use of focus microwave-assisted extraction (FMAE) is proposed. Under optimized conditions, four steroidal saponins were extracted from Tribulus terrestris and identified by GC-MS, which are Tigogenin (TG), Gitogenin (GG), Hecogenin (HG) and Neohecogenin (NG). One of the most important steroidal saponins, namely TG was quantified finally. The recovery of TG was in the range of 86.7-91.9% with RSD<5.2%. The convention heating reflux extraction was also conducted in order to validate the reliability of this new FMAE method. The yield of total steroidal saponins was 90.3% in a one-step FMAE, while the yield of 65.0% was achieved during heating reflux extraction, and the extraction time was reduced from 3 h to 5 min by using less solvent. The method was successfully applied to analyze the steroidal saponins of Tribulus terrestris from different areas of occurrence. The difference in chromatographic characteristics of steroidal saponins was proved to be related to the different areas of occurrence. The results showed that FMAE-GC-MS is a simple, rapid, solvent-saving method for the extraction and determination of steroidal saponins in Tribulus terrestris.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, PR China.
authors.forename: Tianlin
authors.initials: T
authors.lastname: Li
authors.forename: Zhuomin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinjian
authors.initials: X
authors.lastname: Huang
authors.forename: Junwei
authors.initials: J
authors.lastname: Lin
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D010840:Phytosterols / Q000032:analysis / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D012502:Sapogenins / Q000032:analysis / Q000302:isolation & purification; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D013150:Spirostans / Q000032:analysis / Q000302:isolation & purification; D032444:Tribulus / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D010840:Phytosterols; D012502:Sapogenins; D012503:Saponins; D013150:Spirostans; C007908:hecogenin; C012999:gitogenin; C046297:sarsasapogenin