Resistance to the organophosphate temephos: mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil.
Insecticide resistance is one of the main problems in vector control programs. Because insects have developed resistance to all classes of available chemical insecticides, a proper surveillance and management of resistance in areas where these compounds are being utilized is crucial for the success of control programs. Since the mechanisms and molecular bases of resistance are various, they must be characterized to allow efficient monitoring strategies. Here we report the establishment of an Aedes aegypti strain resistant to temephos, named RecR, selected under laboratory conditions. The parental A. aegypti population was obtained from eggs collected in an area where temephos had been used for 8 years, and presented a baseline resistance ratio (RR) of 7. After 17 generations under selective pressure, the RR has increased to 180. Biochemical assays indicate that metabolic mechanisms are involved on temephos resistance in the selected strain. These experiments showed that, compared to the susceptible colony Rockefeller, RecR present higher activity of glutathione S-transferases (GSTs), alpha- and beta-esterases, and, to a lesser degree, mixed function oxidases (MFO). At the 14th or 17th generations, there was no cross resistance of these insects to deltamethrin, cypermethrin and malathion, while a low resistance level (RR=3) was observed for pyriproxyfen, a juvenile hormone analogue. Experiments on resistance reversal, performed through three different field simulated schemes using the resistant strain, showed that temephos susceptibility can be recovered. The establishment of an A. aegypti colony resistant to temephos is extremely valuable for a deeper understanding of resistance mechanisms and thus for further improvements in control strategies against this vector. With the urgent need on improving methodologies to monitor resistance, molecular studies such as microarrays, and resistant colonies such as RecR will certainly hasten such studies.
Melo-Santos
M A V
MA
Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz. Av. Moraes Rego s/n, Cidade Universitária, CEP: 50670-420, Recife PE, Brazil. mavarjal@cpqam.fiocruz.br
Varjal-Melo
J J M
JJ
Araújo
A P
AP
Gomes
T C S
TC
Paiva
M H S
MH
Regis
L N
LN
Furtado
A F
AF
Magalhaes
T
T
Macoris
M L G
ML
Andrighetti
M T M
MT
Ayres
C F J
CF
D000330:Aedes / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D000830:Animals, Laboratory; D001681:Biological Assay; D001938:Brazil; D019020:Directed Molecular Evolution; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007303:Insect Vectors / Q000187:drug effects / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology*; D000002:Temefos / Q000494:pharmacology*
D019476:Insect Proteins; D007306:Insecticides; D000002:Temefos