Time-lapse live imaging of stem cells in Drosophila testis.
This unit describes a protocol for time-lapse live-imaging of stem cells in Drosophila testis. Testis tips are dissected from Drosophila, sliced, and transferred to glass-bottom chambers where the stem cells residing in their native microenvironment can be monitored in real time. This protocol, facilitated with various fluorescence-labeled markers, allows dynamic cellular processes in stem cells to be characterized throughout the cell cycle.
Cheng
Jun
J
University of Michigan, Ann Arbor, Michigan, USA.
Hunt
Alan J
AJ
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002465:Cell Movement; D004331:Drosophila melanogaster / Q000166:cytology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D013234:Stem Cells / Q000166:cytology*; D013737:Testis / Q000378:metabolism*; D013997:Time Factors
D005456:Fluorescent Dyes