Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian species.
Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of nine Japanese amphibian species using reverse transcription polymerase chain reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and anuran amphibians formed two branches. Within the anuran species, three branches were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese amphibians).
Nakayama
Shouta M M
SM
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku N18W9, Sapporo 060-0818, Japan.
Tanaka-Ueno
Tomoko
T
Sakamoto
Kentaro Q
KQ
Fujita
Shoichi
S
Ishizuka
Mayumi
M
D000663:Amphibians / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D007564:Japan
D003577:Cytochrome P-450 Enzyme System