title: Engineering scaffold-free bone tissue using bone marrow stromal cell sheets.
abstract: The use of exogenous scaffolds to engineer bone tissue faces several drawbacks including insufficient biological activity, potential immunogenicity, elevated inflammatory reaction, fluctuating degradation rate, and low cell-attachment efficiency. To circumvent these limitations, we sought to engineer large scaffold-free bone tissue using cell sheets. We harvested intact cell sheets from bone marrow stromal cells using a continuous culture method and a scraping technique. The cell sheets were then rolled and fabricated into large constructs. Finally, the constructs were implanted into the subcutaneous pockets of nude mice. The cells within the sheet maintained in vitro osteogenic potential after osteoblast differentiation. Computed tomography scans and histological examination confirmed new bone formation in vivo. Additionally, the engineered bone exhibited enhanced compressive strength. Our results indicate that the BMSC sheets can facilitate the formation of functional three-dimensional bone tissue without the use of exogenous scaffolds. Hence, the study provides an intriguing alternative strategy for bone repair.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Chang Le 145 West Road, Xi'an, 710032, People's Republic of China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Ma
authors.forename: Liling
authors.initials: L
authors.lastname: Ren
authors.forename: Yanpu
authors.initials: Y
authors.lastname: Liu
authors.forename: Fulin
authors.initials: F
authors.lastname: Chen
authors.forename: Junrui
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhenxun
authors.initials: Z
authors.lastname: Xue
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Mao
mesh_terms: D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods*; D019245:Compressive Strength / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D051847:Lithostathine / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed
chemical_list: D015415:Biomarkers; D051847:Lithostathine