title: Sequence variation in the cytochrome oxidase subunit I and II genes of two commonly found blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) in Malaysia.
abstract: The mitochondiral DNA region encompassing the cytochrome oxidase subunit I (COI) and cytochrome oxidase subunit II (COII) genes of two Malaysian blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) were studied. This region, which spans 2303bp and includes the COI, tRNA leucine and partial COII was sequenced from adult fly and larval specimens, and compared. Intraspecific variations were observed at 0.26% for Ch. megacephala and 0.17% for Ch. rufifacies, while sequence divergence between the two species was recorded at a minimum of 141 out of 2303 sites (6.12%). Results obtained in this study are comparable to published data, and thus support the use of DNA sequence to facilitate and complement morphology-based species identification.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Siew Hwa
authors.initials: SH
authors.lastname: Tan
authors.forename: Edah Mohd
authors.initials: EM
authors.lastname: Aris
authors.forename: Johari
authors.initials: J
authors.lastname: Surin
authors.forename: Baharudin
authors.initials: B
authors.lastname: Omar
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kurahashi
authors.forename: Zulqarnain
authors.initials: Z
authors.lastname: Mohamed
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D004175:Diptera / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D053803:Forensic Genetics; D017344:Genes, Insect; D014644:Genetic Variation; D007814:Larva / Q000235:genetics; D008296:Malaysia; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012356:RNA, Transfer, Leu / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D012356:RNA, Transfer, Leu; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV