title: Study of the adhesion improvement in hippocampal cells on collagen/chitosan- modified silicon surfaces.
abstract: The aim of this study is to improve hippocampal cell adhesion on silicon (Si) surfaces by surface modification via a chemical method. Collagen and chitosan were first immobilized on a Si surface through a bridging layer of (3-aminopropyl) triethoxysilane and 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide. The modified Si surface was then characterized by contact angle measurement, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy and atomic force microscopy. The amounts of protein adsorbed on the bare Si surface and collagen/chitosan-modified Si surface were measured by a modified Coomassie brilliant blue protein assay. The cell adhesion behavior was then assessed by a microscopy. The results showed that after the surface modification with the collagen/chitosan compound the protein adsorption on Si surfaces decreased while the adhesion ability of hippocampal cells increased.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Lü
authors.forename: Zhigong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhenglin
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods; D011437:Propylamines; D051381:Rats; D017207:Rats, Sprague-Dawley; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D001672:Biocompatible Materials; D002234:Carbodiimides; D011437:Propylamines; D012821:Silanes; D003094:Collagen; D048271:Chitosan; C477625:amino-propyl-triethoxysilane; D012825:Silicon