Kaempferol glycosides with antioxidant activity from Brassica juncea.
From the leaves of Brassica juncea, three kaempferol glycosides, kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), kaempferol-3-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (2), and kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) were isolated and the structures elucidated on the basis of spectral and chemical evidences. Antioxidant activities were determined by measuring the scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO(-)). Compounds 1 and 3 showed good antioxidant activities with respective IC(50) values of 28.61 and 36.93 microM toward DPPH; respective IC(50) values of 9.79 and 11.40 microM toward ONOO(-). However, compound 2 showed no DPPH scavenging activity and weak ONOO(-) scavenging activity with an IC(50) value of 32.00 microM.
Jung
Hyun Ah
HA
Division of Food Science and Biotechnology, Pukyong National University, Busan, Korea.
Woo
Ju Jung
JJ
Jung
Mee Jung
MJ
Hwang
Geum-Sook
GS
Choi
Jae Sue
JS
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D009149:Mustard Plant / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D006027:Glycosides; D044949:Kaempferols; D010851:Picrates; D030421:Peroxynitrous Acid; C006552:kaempferol; C004931:1,1-diphenyl-2-picrylhydrazyl