title: Long fluorescence lifetime molecular probes based on near infrared pyrrolopyrrole cyanine fluorophores for in vivo imaging.
abstract: Fluorescence lifetime (FLT) properties of organic molecules provide a new reporting strategy for molecular imaging in the near infrared (NIR) spectral region. Unfortunately, most of the NIR fluorescent dyes have short FLT typically clustered below 1.5 ns. In this study, we demonstrate that a new class of NIR fluorescent dyes, pyrrolopyrrole cyanine dyes, have exceptionally long FLTs ranging from 3 to 4 ns, both in vitro (dimethyl sulfoxide and albumin/water solutions) and in vivo (mice). These results provide a new window for imaging molecular processes, rejecting backscattered light and autofluorescence, and multiplexing imaging information with conventional NIR fluorescent dyes that absorb and emit light at similar wavelengths.
authors.affiliation: Department of Radiology, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Berezin
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Akers
authors.forename: Kevin
authors.initials: K
authors.lastname: Guo
authors.forename: Georg M
authors.initials: GM
authors.lastname: Fischer
authors.forename: Ewald
authors.initials: E
authors.lastname: Daltrozzo
authors.forename: Andreas
authors.initials: A
authors.lastname: Zumbusch
authors.forename: Samuel
authors.initials: S
authors.lastname: Achilefu
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D002232:Carbocyanines / Q000494:pharmacology*; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008027:Light; D051379:Mice; D008956:Models, Chemical; D011758:Pyrroles / Q000494:pharmacology*; D012542:Scattering, Radiation; D012997:Solvents / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D000418:Albumins; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011758:Pyrroles; D012997:Solvents; D014867:Water; D004121:Dimethyl Sulfoxide