Cadmium exposure modifies lactotrophs activity associated to genomic and morphological changes in rat pituitary anterior lobe.
Cadmium (Cd) is widely used in industrial applications and is an important contaminant of agricultural products. As an endocrine disruptor, Cd modifies the hormone release of pituitary anterior lobe (PAL). This work was undertaken to evaluate a possible association between phospholipase D (PLD) and prolactin mRNA expressions and the activity of lactotrophs and folliculostellate cells (FSC) in PAL of Cd exposed adult male Wistar rats (Cd, 0.133 mM per liter for 2 months). The PALs were submitted to immunohistochemical and morphometric analysis to determine the percentage of lactotrophs (PRL-ir) and FSC (S-100-ir). Cultured PAL cells were stained with Hoechst 33258 to determine the presence of alterations in nuclear morphology consistent with apoptosis. The expressions of PLD and prolactin mRNA were assessed by RT-PCR. Cd treated rats showed a decrease of PLD mRNA levels that can be associated to both high number of apoptotic cells and increase of S-100 protein expression in FSC. Cd decreased prolactin mRNA expression, number of lactotrophs and percentage of PRL-ir suggesting a low availability of prolactin to be secreted from PAL. Cd modifies the lactotrophs activity of pituitary gland through biochemical, genomic and morphological changes and contributes directly or indirectly to the levels of serum prolactin.
Calderoni
Ana María
AM
Laboratorio de Química Biológica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, IMIBIO-SL (CONICET), Avenida Ejército de los Andes 950, D5700HHW San Luis, Argentina.
Biaggio
Verónica
V
Acosta
Mariano
M
Oliveros
Liliana
L
Mohamed
Fabian
F
Giménez
María Sofía
MS
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019256:Cadmium Chloride / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011388:Prolactin / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship
D012333:RNA, Messenger; D011388:Prolactin; D010739:Phospholipase D; D019256:Cadmium Chloride