Contributions of ion channel currents to ventricular action potential changes and induction of early afterdepolarizations during acute hypoxia.
BACKGROUND
Variability in delivery of oxygen can lead to electric instability in the myocardium and the generation of arrhythmias. In addition ischemic heart disease and angina are associated with an increase in circulating catecholamines that further increases the risk of developing ventricular tachyarrhythmias.


OBJECTIVE
We investigated the net effects of acute hypoxia and catecholamines on the cardiac action potential.


RESULTS
We incorporated all published data on the effects of hypoxia on the late Na(+) current (I(Na-L)), the fast Na(+) current (I(Na)), the basal L-type Ca(2+) channel current (I(Ca-L)), and the slow (I(Ks)) and rapid components of the delayed rectifier K(+)-current (I(Kr)) in the absence and presence of beta-adrenergic receptor (beta-AR) stimulation into the Luo-Rudy model of the action potential. Hypoxia alone had little effect on the action potential configuration or action potential duration. However in the presence of beta-AR stimulation, hypoxia caused a prolongation of the action potential and early afterdepolarizations (EADs) and spontaneous tachycardia were induced. Experiments performed in guinea pig ventricular myocytes confirmed the modeling results.


CONCLUSIONS
EADs occur predominantly because of the increased sensitivity of I(Ca-L) to beta-AR stimulation during hypoxia. beta-AR stimulation is necessary to induce EADs as EADs are never observed during hypoxia in the absence of beta-AR stimulation.
Gaur
Namit
N
Physiology M311, School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Rudy
Yoram
Y
Hool
Livia
L
D000200:Action Potentials; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020013:Calcium Signaling; D015687:Cell Hypoxia; D051668:Delayed Rectifier Potassium Channels / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D008955:Models, Cardiovascular; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism; D013997:Time Factors
D000318:Adrenergic beta-Agonists; D020746:Calcium Channels, L-Type; D051668:Delayed Rectifier Potassium Channels; D011943:Receptors, Adrenergic, beta; D015222:Sodium Channels; D007545:Isoproterenol; D010100:Oxygen