title: Evaluation of a dengue IgG indirect enzyme-linked immunosorbent assay and a Japanese encephalitis IgG indirect enzyme-linked immunosorbent assay for diagnosis of secondary dengue virus infection.
abstract: To establish a new method for the diagnosis of dengue secondary infection, 187 serum samples from the patients with dengue secondary infection, 40 serum samples from the patients with dengue primary infection, and 44 serum samples from the healthy volunteers were tested using the dengue IgG indirect enzyme-linked immunosorbent assay (DEN IgG ELISA). The results of the test were compared with those from the dengue hemagglutination inhibition (DEN HI) test, which has been recommended as the gold standard by the World Health Organization (WHO, 1997). Japanese encephalitis IgG indirect ELISA (JE IgG ELISA) was also performed to measure anti-flavivirus IgG, which cross-reacts with the Japanese encephalitis virus, to test the possibility of an alternative to DEN IgG ELISA. The results of DEN IgG and JE IgG ELISAs were highly correlated with those of the DEN HI test. In the DEN IgG ELISA, a titer of 1:29,000 was the cut-off value for the diagnosis of dengue secondary infection (91.5% accuracy [95% confidence interval, CI], 90.9% sensitivity [95%CI], and 92.9% specificity [95%CI]). A titer of 1:52,000 was the cut-off value for dengue secondary infection using JE IgG ELISA (95.6% accuracy [95%CI], 98.9% sensitivity [95%CI], and 88.1% specificity [95%CI]). In conclusion, this study confirmed that the results of both DEN IgG and JE IgG ELISAs were highly correlated with the results of DEN HI test. Thus, these ELISAs are simple, rapid, sensitive, and quantitative tests that can be used in the determination of dengue secondary infection.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Inoue
authors.forename: Maria T G
authors.initials: MT
authors.lastname: Alonzo
authors.forename: Yae
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Mapua
authors.forename: Joyce D
authors.initials: JD
authors.lastname: Reyes
authors.forename: Efren M
authors.initials: EM
authors.lastname: Dimaano
authors.forename: Maria Theresa P
authors.initials: MT
authors.lastname: Alera
authors.forename: Mariko
authors.initials: M
authors.lastname: Saito
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
authors.forename: Futoshi
authors.initials: F
authors.lastname: Hasebe
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Matias
authors.forename: Filipinas F
authors.initials: FF
authors.lastname: Natividad
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D000918:Antibody Specificity; D016022:Case-Control Studies; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G