Resonance assignments of the human AKAP13-PH domain and stabilizing DH helix.
The human AKAP13 protein contains DH and PH domains, which are responsible for its cell transforming activity. Despite its biomedical importance, the contribution of the PH domain to AKAP13 activity remains unclear and no three dimensional structure is available to date. Here we report the backbone and side chain (1)H, (13)C and (15)N resonance assignments of a 20 kDa construct comprising the uniformly (13)C and( 15)N labeled AKAP13-PH domain and an associated helix from the DH domain which is required for its stable expression. Resonance assignment has been achieved using conventional triple resonance experiments; 95% of all back bone resonances and more than 90% of side chain resonances have been successfully assigned. The (1)H, (13)C and (15)N chemical shifts have been deposited in BMRB with accession number of 16195.
Sugawara
Masae
M
School of Cancer Sciences, University of Birmingham, Birmingham, B15 2TT, UK. m.sugawara@bham.ac.uk
Whittaker
Sara B-M
SB
Bishop
Shurene
S
Ball
Linda
L
Overduin
Michael
M
D054758:A Kinase Anchor Proteins / Q000737:chemistry*; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010750:Phosphoproteins / Q000737:chemistry*; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
D054758:A Kinase Anchor Proteins; C085467:AKAP13 protein, human; D001798:Blood Proteins; D004268:DNA-Binding Proteins; D015778:Minor Histocompatibility Antigens; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; C088395:VEZF1 protein, human; C039077:platelet protein P47