Serum and tissue profiling in bladder cancer combining protein and tissue arrays.
Aiming at identifying biomarkers for bladder cancer, the serum proteome was explored in a pilot study through a profiling approach using protein arrays. Supervised analyses identified a panel 171 immunogenic proteins differentially expressed between patients with bladder cancer (n = 12) and controls without the disease (n = 10). The microanatomical expression patterns of novel immunogenic proteins, especially dynamin and clusterin, were found significantly associated with histopathologic variables and overall survival, as confirmed by immunohistochemistry using an independent series of bladder tumors contained in tissue microarrays (n = 289). Thus, the protein arrays approach has identified a panel of immunogenic candidates that may potentially play a role as diagnostic biomarkers, especially for muscle invasive disease. Moreover, the protein expression patterns of dynamin and clusterin in bladder tumors were shown to adjunct for histopathologic staging and clinical outcome prognosis.
Orenes-Piñero
Esteban
E
Tumor Markers Group, Spanish National Cancer Research Center, Madrid, Spain.
Barderas
Rodrigo
R
Rico
Daniel
D
Casal
J Ignacio
JI
Gonzalez-Pisano
David
D
Navajo
Jose
J
Algaba
Ferran
F
Piulats
Josep Maria
JM
Sanchez-Carbayo
Marta
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood; D002295:Carcinoma, Transitional Cell / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D051152:Clusterin / Q000032:analysis / Q000096:biosynthesis; D018450:Disease Progression; D034281:Dynamins / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D046888:Tissue Array Analysis / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000276:immunology / Q000378:metabolism*
D001323:Autoantibodies; D051152:Clusterin; D009363:Neoplasm Proteins; D034281:Dynamins