[A method of laser dark field illumination for equal-probability excitation and photobleaching of fluorescent molecules with arbitrary orientation of their light absorbing oscillators].
Calculations have been done, which allow one to develop a new type of illuminators optimized for dark field microscopy and Fluorescent Nanoscopy--a new type of superresolution microscopy for which we have patent priority and its later developed analogs. The concept proposed in this article enables one to develop an illumination system necessary for Fluorescent Nanoscopy, which provides the illumination of different parts of an object with equal probability of excitation and, after some delay, equal probability of photobleaching of fluorescent molecules with arbitrary orientation of their light absorbing oscillators. The latter is important because non-bleached fluorescent molecules that accumulate in the specimen produce an background noise and make it too difficult to find centers of spots produced by new fluorescent molecules.
Klimov
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Klimov
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D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D038761:Photobleaching
D005456:Fluorescent Dyes