title: Twins born after transplantation of ovarian cortical tissue and oocyte vitrification.
abstract: OBJECTIVE
  To present a combination of ovarian tissue and oocyte cryopreservation as an effective strategy for achieving pregnancy in a breast cancer patient.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Tertiary care university-affiliated hospital, tissue bank, and infertility clinic.
  
  
  METHODS
  A 36-year-old patient diagnosed with atypical medullar breast cancer and negative for estrogen, P, and HER2 receptors underwent ovarian tissue cryopreservation before receiving chemotherapy and radiotherapy.
  
  
  METHODS
  Laparoscopic ovarian cortex extraction, ovarian tissue cryopreservation, ovarian tissue thawing and transplantation, controlled ovarian stimulation (COS), oocyte retrieval, vitrification and IVF, and embryo culture and replacement.
  
  
  METHODS
  Resumption of spontaneous ovarian function after transplantation, response to COS, oocyte vitrification, IVF, pregnancy, and delivery.
  
  
  RESULTS
  Menses occurred 63 days after transplantation. Sixteen mature oocytes were obtained in four COS procedures. All vitrified oocytes survived warming, and 77.7% were fertilized. Two day 3 embryos were replaced, and two healthy boys were born at 34 weeks.
  
  
  CONCLUSIONS
  Ovarian tissue cryopreservation and grafting preserves fertility. Simultaneous oocyte vitrification increases the success of assisted reproductive technology in poor-prognosis patients and avoids the consequences of the short lifespan of the transplanted tissue.
authors.affiliation: Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Sánchez-Serrano
authors.forename: Juana
authors.initials: J
authors.lastname: Crespo
authors.forename: Vicente
authors.initials: V
authors.lastname: Mirabet
authors.forename: Ana C
authors.initials: AC
authors.lastname: Cobo
authors.forename: María-José
authors.initials: MJ
authors.lastname: Escribá
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000503:physiopathology / Q000628:therapy*; D015925:Cryopreservation; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes; D010053:Ovary / Q000637:transplantation*; D010062:Ovulation Induction; D011247:Pregnancy; D016896:Treatment Outcome; D014427:Twins