title: Immobilized-metal affinity chromatography (IMAC): a review.
abstract: This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application-purification of histidine-tagged recombinant proteins-will be reviewed in greater detail with focus of state-of-the-art materials, methods, and protocols, and the limitations of IMAC and recent advances to improve the technology and the methods will be described.
authors.affiliation: QIAGEN GmbH, Qiagen Strasse 1, Hilden, Germany.
authors.forename: Helena
authors.initials: H
authors.lastname: Block
authors.forename: Barbara
authors.initials: B
authors.lastname: Maertens
authors.forename: Anne
authors.initials: A
authors.lastname: Spriestersbach
authors.forename: Nicole
authors.initials: N
authors.lastname: Brinker
authors.forename: Jan
authors.initials: J
authors.lastname: Kubicek
authors.forename: Roland
authors.initials: R
authors.lastname: Fabis
authors.forename: Jörg
authors.initials: J
authors.lastname: Labahn
authors.forename: Frank
authors.initials: F
authors.lastname: Schäfer
mesh_terms: D000818:Animals; D001331:Automation / Q000295:instrumentation / Q000379:methods; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D017743:Equipment Reuse; D006801:Humans; D008670:Metals / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D008670:Metals; D011506:Proteins