title: POU6F1 is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary.
abstract: Clear cell adenocarcinoma of the ovary often shows resistance to anticancer agents. We investigated new molecules to use when developing molecular-targeting therapy for clear cell adenocarcinoma of the ovary. RMG-I cells without invasive potential and RMG-V cells with invasive potential (derived from clear cell adenocarcinoma of the ovary) were subjected to complementary deoxyribonucleic acid microarray analysis. Caveolin-1, a molecule involved in cellular motility and invasion, showed differing expression between the two cell lines. An RNA interference experiment using the published siRNA for caveolin-1 was carried out. The results showed suppression of RMG-V cell infiltration by siRNA, but proliferation of the cancer cells was also suppressed. In other words, RMG-V cell infiltration may have been suppressed simply because cell proliferation was suppressed by RNA interference. These findings suggested that POU6F1 might be a transcription factor involved in the proliferation of ovarian cancer cells. Clear cell adenocarcinoma of the ovary shows little response to standard therapy. The results of the present study suggest that the transcription factor POU6F1 could be a new molecular target for treatment of this cancer.
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University, Kanagawa, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Yoshioka
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uekawa
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Bunpei
authors.initials: B
authors.lastname: Ishizuka
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics / Q000473:pathology*; D051242:Caveolin 1 / Q000378:metabolism / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D050809:POU Domain Factors / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D051242:Caveolin 1; D050809:POU Domain Factors; C082703:POU6F1 protein, human; D014157:Transcription Factors