Expression of matrix metalloproteinase-26 in multiple human cancer tissues and smooth muscle cells.
OBJECTIVE
Elevated expression of matrix metalloproteinases (MMPs) has been found in multiple carcinoma tissues. MMP-26 is highly expressed in prostate and breast cancer tissues, and promotes the invasion of human prostate cancer cells not only through the cleavage of fibronectin and type IV collagen but also by the activation of pro-MMP-9, a powerful gelatinase. This study was to present a comprehensive protein expression profile of MMP-26 in multiple human cancer tissues.


METHODS
The protein expression pattern of MMP-26 was examined using immunohistochemistry and multiple-tissue microarray. MMP-26 mRNA expression in coronary artery smooth muscle cells was detected by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The expression of MMP-26 in breast, colon, lung, brain, head and neck, prostate cancer, and melanoma tissues was significantly elevated when compared with parallel normal tissues (P<0.05), while not significantly elevated in kidney cancer, ovarian cancer, and non-Hodgkin's lymphoma (P>0.05). MMP-26 was also detected to express in gastric, rectal, thyroid, esophageal, and pancreatic cancers. MMP-26 protein was expressed in smooth muscle cells of the prostate and associated blood vessels. MMP-26 mRNA was also detected to express in human coronary artery smooth muscle cells.


CONCLUSIONS
MMP-26 expression may be associated with multiple human carcinomas, and it may serve as a molecular marker for the early diagnosis of these carcinomas. MMP-26 may also contribute to smooth muscle function in the human prostate and cardiovascular system.
Zhao
Yun-Ge
YG
Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA. yz5u@virginia.edu
Xiao
Ai-Zhen
AZ
Ni
Jian
J
Man
Yan-Gao
YG
Sang
Qing-Xiang Amy
QX
D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology; D003331:Coronary Vessels / Q000166:cytology / Q000201:enzymology; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis / Q000379:methods; D032389:Myocytes, Smooth Muscle / Q000201:enzymology*; D009367:Neoplasm Staging; D009369:Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D011467:Prostate / Q000166:cytology / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted