Analysis of the cytoprotective effect of amifostine on the irradiated inner ear of guinea pigs: an experimental study.
Radiation can cause damage to the inner ear, from a simple hearing loss all the way to profound deafness. Amifostine is a cytoprotective substance extensively used during radio-chemotherapy for malignant tumors.


OBJECTIVE
the objective of the present investigation was to establish the antioxidant and radioprotective effects of amifostine on the organ of Corti of albino guinea pigs irradiated in the head and neck region.


METHODS
An experimental study conducted on four groups of guinea pigs were used; One group received only amifostine, one group was submitted to a single dose of 350 cGy and the other two were similarly irradiated but received amifostine doses of 100 or 200 mg/kg. All animals were slaughtered 30 days after the experiment, their bullae were removed and the damaged outer hair cells were counted.


RESULTS
The extent of injury was lower in the outer hair cells of the two groups treated with amifostine compared to the group that was only irradiated. There was no difference between the group treated with 100 and 200 mg/kg of amifostine. The group that received only amifostine had no cochlear damage.


CONCLUSIONS
Amifostine is an effective cytoprotective substance in the Organ of Corti of irradiated guinea pigs.
Lessa
Ricardo Miranda
RM
University Hospital, Medical School at Ribeirão Preto, University of São Paulo.
Oliveira
José Antônio Aparecido de
JA
Rossato
Maria
M
Ghilardi Netto
Thomaz
T
D004999:Amifostine / Q000008:administration & dosage*; D000818:Animals; D006168:Guinea Pigs; D008297:Male; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000528:radiation effects* / Q000648:ultrastructure; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage*
D011837:Radiation-Protective Agents; D004999:Amifostine