Molecular typing of Staphylococcus aureus strains from ovine mastitis by pulsed-field gel electrophoresis and polymerase chain reaction based on coagulase and protein A gene polymorphisms.
Staphylococcus aureus is one of the most important etiologic agents of ovine mastitis. To develop effective control measures for mastitis, it is important to type S. aureus strains that have considerable genetic heterogeneity. In the current study, 47 S. aureus strains isolated from ovine mastitis were typed by polymerase chain reaction (PCR) based on coagulase (coa) and protein A (spa) polymorphisms and by pulsed-field gel electrophoresis (PFGE). Eight different coa types and 4 spa types were identified by PCR. While the most prevalent coa type was CG2 (42.56%), the spa types S4 and S1 were the most commonly observed (44.68% and 38.29%, respectively). Nineteen different pulsotypes were identified, and 12 of these were represented by a single isolate. Pulsotypes J and K were predominant and each represented 9 isolates (19.14%). All isolates belonging to J and K pulsotypes were CG2. Although all 9 isolates belonging to the J pulsotype were S4, all isolates in the K pulsotype were S1. While PFGE was found to be the best discriminatory technique for distinguishing strains, coa and spa types were found to be in correlation with PFGE types and can be used for quick, preliminary epidemiologic studies for detecting strains that may cause mastitis.
Ciftci
Alper
A
University of Ondokuz Mayis, Faculty of Veterinary Medicine, Department of Microbiology, 55139 Kurupelit, Samsun, Turkey. aciftci@omu.edu.tr
Onuk
E Emek
EE
Findik
Arzu
A
Yildirim
Tuba
T
Sogut
Mehtap Unlu
MU
D000818:Animals; D003030:Coagulase / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D013203:Staphylococcal Infections / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
D003030:Coagulase; D013205:Staphylococcal Protein A