Interaction of afobazole with sigma1-receptors.
In vitro radioligand assay revealed interaction of afobazole with sigma(1)-receptors (Ki=5.9x10(-6) M). Translocation of sigma(1)-receptors from the endoplasmic reticulum to the outer membrane was demonstrated by confocal microscopy. Experiments were performed on the model of HT-22 immortalized hippocampal cells after incubation with afobazole in a concentration of 10(-8) M.
Seredenin
S B
SB
V. V. Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, M. V. Lomonosov Moscow State University, Russia.
Antipova
T A
TA
Voronin
M V
MV
Kurchashova
S Yu
SY
Kuimov
A N
AN
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002461:Cell Line, Transformed; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D009025:Morpholines / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011869:Radioligand Assay; D017480:Receptors, sigma / Q000187:drug effects*; D000097605:Sigma-1 Receptor
D014151:Anti-Anxiety Agents; D001562:Benzimidazoles; D009025:Morpholines; D018696:Neuroprotective Agents; D017480:Receptors, sigma; D000097605:Sigma-1 Receptor; C410924:2-((2-morpholino)ethylthio)-5-ethoxybenzimidazole