Elution of proteins from gels.
New protein biochemical technologies have been developed that allow one to learn more and more about a protein with less and less material (on the order of microg). Polyacrylamide gel electrophoresis, which was originally strictly an analytical method, has in some cases become a high-resolution preparative method. This chapter will focus on the elution of proteins from SDS gels, with an emphasis on recovering enzyme or biological activity of the resulting protein.
Burgess
Richard R
RR
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin, USA.
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004058:Diffusion; D004789:Enzyme Activation; D004795:Enzyme Stability / Q000502:physiology; D006801:Humans; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification
D000180:Acrylic Resins; D011506:Proteins; C016680:polyacrylamide gels; D012967:Sodium Dodecyl Sulfate