Blood-feeding and immunogenic Aedes aegypti saliva proteins.
Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.
Wasinpiyamongkol
Ladawan
L
Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Patramool
Sirilaksana
S
Luplertlop
Natthanej
N
Surasombatpattana
Pornapat
P
Doucoure
Souleymane
S
Mouchet
François
F
Séveno
Martial
M
Remoue
Franck
F
Demettre
Edith
E
Brizard
Jean-Paul
JP
Jouin
Patrick
P
Biron
David G
DG
Thomas
Frédéric
F
Missé
Dorothée
D
D000330:Aedes / Q000737:chemistry* / Q000276:immunology; D000821:Animal Feed; D000818:Animals; D001769:Blood; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019476:Insect Proteins / Q000032:analysis* / Q000276:immunology; D012469:Salivary Glands / Q000737:chemistry / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000276:immunology
D019476:Insect Proteins; D012471:Salivary Proteins and Peptides