title: Biological nano-functionalization of titanium-based biomaterial surfaces: a flexible toolbox.
abstract: Surface functionalization with bioactive molecules (BAMs) on a nanometre scale is a main field in current biomaterial research. The immobilization of a vast number of substances and molecules, ranging from inorganic calcium phosphate phases up to peptides and proteins, has been investigated throughout recent decades. However, in vitro and in vivo results are heterogeneous. This may be at least partially attributed to the limits of the applied immobilization methods. Therefore, this paper highlights, in the first part, advantages and limits of the currently applied methods for the biological nano-functionalization of titanium-based biomaterial surfaces. The second part describes a new immobilization system recently developed in our groups. It uses the nanomechanical fixation of at least partially single-stranded nucleic acids (NAs) into an anodic titanium oxide layer as an immobilization principle and their hybridization ability for the functionalization of the surface with BAMs conjugated to the respective complementary NA strands.
authors.affiliation: Max Bergmann Center of Biomaterials, TU Dresden, Budapester Strasse 27, 01069 Dresden, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Beutner
authors.forename: Jan
authors.initials: J
authors.lastname: Michael
authors.forename: Bernd
authors.initials: B
authors.lastname: Schwenzer
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000737:chemistry*; D004563:Electrochemistry; D055767:Immobilized Proteins / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods* / Q000639:trends; D009696:Nucleic Acids / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D009696:Nucleic Acids; C009495:titanium dioxide; D014025:Titanium