[Identification and comparison of constituents in HPLC fingerprint of five Chinese herbal pieces from Rheum palamatum].
OBJECTIVE
To identificate and compare constituents in the HPLC fingerprint of five Chinese herbal pieces from Radix et Rhizoma Rhei.


METHODS
HPLC analysis was carried out with methyl alcohol 1% glacial acetic acid as gradient elution, changes in five Chinese herbal pieces and medicinal material under 280 nm and 430 nm were compared.


RESULTS
HPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces were similar, 24 peaks were identified under 280 nm 19 or 22 peaks could be indicated in the braising with liquor and the charring respectively. Under 430 nm, 8 peaks were identified except the braising with liquor.


CONCLUSIONS
HPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces are similar while the changes on chemical composition and the content in braising with liquor and the charring are remarkable.
Li
Li
L
Institute of Chinese Material Medica, China Academy of Chinese Medicines, Beijing 100700, China.
Zhang
Cun
C
Xiao
Yongqing
Y
Lin
Na
N
Liu
Chunfang
C
Pang
Zhen
Z
Li
Guiliu
G
Chen
Dongdong
D
Tian
Guofang
G
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D012250:Rheum / Q000737:chemistry*
D004365:Drugs, Chinese Herbal