title: Plasticity and recovery of skeletal muscle satellite cells during limb regeneration.
abstract: Salamander limb regeneration depends on local progenitors whose progeny are recruited to the new limb. We previously identified a Pax7(+) cell population in skeletal muscle whose progeny have the potential to contribute to the regenerating limb. However, the plasticity of individual Pax7(+) cells, as well as their recovery within the new limb, was unclear. Here, we show that Pax7(+) cells remain present after multiple rounds of limb amputation/regeneration. Pax7(+) cells are found exclusively within skeletal muscle in the regenerating limb and proliferate where the myofibers are growing. Pax7 is rapidly down-regulated in the blastema, and analyses of clonal derivatives show that Pax7(+) cell progeny are not restricted to skeletal muscle during limb regeneration. Our data suggest that the newt regeneration blastema is not entirely a composite of lineage-restricted progenitors. The results demonstrate that except for a transient and subsequently blunted increase, skeletal muscle satellite cells constitute a stable pool of reserve cells for multiple limb regeneration events.-Morrison, J. I., Borg, P., Simon, A. Plasticity and recovery of skeletal muscle satellite cells during limb regeneration.
authors.affiliation: A.S., Karolinska Institute, Department of Cell and Molecular Biology, von Eulersväg 3, 17177 Stockholm, Sweden.
authors.forename: Jamie I
authors.initials: JI
authors.lastname: Morrison
authors.forename: Paula
authors.initials: P
authors.lastname: Borg
authors.forename: András
authors.initials: A
authors.lastname: Simon
mesh_terms: D000818:Animals; D005121:Extremities / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D051763:PAX7 Transcription Factor / Q000378:metabolism; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism / Q000502:physiology*; D014562:Caudata
chemical_list: D051763:PAX7 Transcription Factor