title: Blood-feeding and immunogenic Aedes aegypti saliva proteins.
abstract: Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.
authors.affiliation: Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Ladawan
authors.initials: L
authors.lastname: Wasinpiyamongkol
authors.forename: Sirilaksana
authors.initials: S
authors.lastname: Patramool
authors.forename: Natthanej
authors.initials: N
authors.lastname: Luplertlop
authors.forename: Pornapat
authors.initials: P
authors.lastname: Surasombatpattana
authors.forename: Souleymane
authors.initials: S
authors.lastname: Doucoure
authors.forename: François
authors.initials: F
authors.lastname: Mouchet
authors.forename: Martial
authors.initials: M
authors.lastname: Séveno
authors.forename: Franck
authors.initials: F
authors.lastname: Remoue
authors.forename: Edith
authors.initials: E
authors.lastname: Demettre
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Brizard
authors.forename: Patrick
authors.initials: P
authors.lastname: Jouin
authors.forename: David G
authors.initials: DG
authors.lastname: Biron
authors.forename: Frédéric
authors.initials: F
authors.lastname: Thomas
authors.forename: Dorothée
authors.initials: D
authors.lastname: Missé
mesh_terms: D000330:Aedes / Q000737:chemistry* / Q000276:immunology; D000821:Animal Feed; D000818:Animals; D001769:Blood; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019476:Insect Proteins / Q000032:analysis* / Q000276:immunology; D012469:Salivary Glands / Q000737:chemistry / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000276:immunology
chemical_list: D019476:Insect Proteins; D012471:Salivary Proteins and Peptides