title: THE STABILITY OF BACTERIAL SUSPENSIONS : IV. THE COMBINATION OF ANTIGEN AND ANTIBODY AT DIFFERENT HYDROGEN ION CONCENTRATIONS.
abstract: 1. The amount of immune body required to agglutinate a suspension of Bacillus typhosus increases in direct proportion to the concentration of the suspension. 2. The amount of immune body combined with the organisms is constant from pH 9 to pH 3.7. Below the latter value the amount in combination is decreased. 3. The addition of immune serum to a suspension of Bacillus typhosus at a pH of 2,5 increases the positive charge of the organisms. These results are contradictory to the idea that the combination is caused by a difference in the sign of the charge carried by the immune body and the organism. They agree with the assumption that the immune body forms a film on the surface of the organism and that the effect on the charge is the result of this film.
authors.affiliation: Laboratories of The Rockefeller Institute for Medical Research.
authors.forename: P H
authors.initials: PH
authors.lastname: De Kruif
authors.forename: J H
authors.initials: JH
authors.lastname: Northrop