[Determination of the eliminating ratio of TCM polysaccharide to hydroxyl radical by kinetic fluorescent spectrophotometry].
OBJECTIVE
To explore reaction dynamic of hydroxyl radical (*OH) and salicylic acid,and to determine the elimination ratios of TCM polysaccharide to *OH by kinetic fluorescent analysis.


METHODS
The impact dynamics factors of this reaction were studied by fluorescent, such as the reaction of concentration, reaction time and temperature. The dynamical equation was built, a kinetic fluorescent spectrophotometry based on the reaction was developed to determine the elimination ratio. Repetitiveness and reliability of this method were tested by vitamin C.


RESULTS
The dynamical equation of reaction rate to salicylic acid was gamma = 0. 9818x -1. 1801 under the condition of lambda ex = 295 nm, lambda em = 411 nm at room temperature, r approximately 1. The 50% elimination ratio (IC50) of TCM polysaccharide of Tangerine peel and Ganoderma lucidum to *OH was 78.01 mg/L and 232.5 mg/L, respectively. The IC50 of vitamin C was 24.52 microg/L, RSD was 0.23% (n = 5).


CONCLUSIONS
The method is sensitive and reliable, it can be used to determine the elimination ratio of TCM polysaccharide to *OH.
Feng
Hui-Ping
HP
Department of Analytical Chemistry, Guangdong Pharmaceutical University, Guangzhou 510006, China. huipingf@126.com
Mo
Yun-Yan
YY
Huang
Min-Si
MS
Pan
Yong-Qiang
YQ
Fan
Hua-Jun
HJ
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers; D032902:Ganoderma / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020156:Salicylic Acid / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D017665:Hydroxyl Radical; D020156:Salicylic Acid