[Ru(bpy)2dppz]2+ electrochemiluminescence switch and its applications for DNA interaction study and label-free ATP aptasensor.
[Ru(bpy)2dppz]2+ electrochemiluminescence (ECL) was studied, and it was used to investigate DNA interaction and develop a label-free ATP aptasensor for the first time. ECL of [Ru(bpy)2dppz]2+ is negligible in aqueous solution, and increases approximately 1000 times when [Ru(bpy)2dppz]2+ intercalates into the nucleic acid structure. The ECL switch behavior of [Ru(bpy)2dppz]2+ is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. The ECL switch by DNA was applied to investigate the interaction of [Ru(bpy)2dppz]2+ with herring sperm DNA. The calculated equilibrium constant (K) is 1.35 x 10(6) M(-1), and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. Moreover, ATP can dramatically affect ECL of the [Ru(bpy)2dppz]2+/ATP aptamer complex. As a result, a label-free, sensitive, and selective [Ru(bpy)2dppz]2+ ECL method for ATP detection was developed. The detection limit is 100 nM for ATP (with a signal-to-noise ratio, S/N, of 3) with a linear range of 0-1 microM. The result demonstrates that [Ru(bpy)2dppz]2+ ECL holds great promise in aptasensors.
Hu
Lianzhe
L
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, People's Republic of China.
Bian
Zheng
Z
Li
Haijuan
H
Han
Shuang
S
Yuan
Yali
Y
Gao
Lianxun
L
Xu
Guobao
G
D000255:Adenosine Triphosphate / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010619:Phenazines / Q000737:chemistry* / Q000378:metabolism*; D013194:Staining and Labeling
D052157:Aptamers, Nucleotide; D007364:Intercalating Agents; D009942:Organometallic Compounds; D010619:Phenazines; C077849:bis(2,2'-bipyridyl)(dipyrido(3,2-alpha-2',3'-c)phenazine)ruthenium (II); D000255:Adenosine Triphosphate; D004247:DNA