Elicitation of broad CTL response against HIV-1 by the DNA vaccine encoding artificial multi-component fusion protein MultiHIV--study in domestic pigs.
Broad CTL response against HIV-1 is one factor that helps to control the viral replication. We have constructed a DNA vaccine that encodes a large artificial fusion protein (MultiHIV) and shown it to be immunogenic in mice, swine and macaques. Inbred mice revealed CTL response only against two epitopes due to limited MHC class I variability. To assess the quality of the CTL response we addressed this question in domestic swine. Number of presented epitopes varied between 7 and 14 among the five selected animals. Epitopes detected in swine are localised in the same antigenic regions recognised in humans. This can be explained by the fact that swine MHC-I (SLA-I) complex is remarkably similar to human HLA-I. These results also indicate that immunogenicity profile of vaccines in domestic swine may predict the outcome of human immunisation.
Mölder
Tarmo
T
FIT Biotech Oy, Tartu, Estonia. tarmo.molder@ut.ee
Adojaan
Maarja
M
Kaldma
Katrin
K
Ustav
Mart
M
Sikut
Rein
R
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D016915:AIDS Vaccines; D000939:Epitopes; D019444:Vaccines, DNA