Inflammatory mediators and posterior segment involvement in ocular Behcet disease.
OBJECTIVE
To assess the serum level of mediators and their relation with posterior ocular involvement in patients with Behcet disease (BD).


METHODS
This study was a prospective, institutional clinical trial including 43 patients with ocular BD. Patients were divided into two subgroups: those with active posterior segment involvement (Group A) and those with inactive ocular BD (Group B). Group A included patients with posterior uveitis (vitritis or chorioretinal involvement), while Group B included patients with no active intraocular inflammation within the last 3 months. Serum levels of interleukin (IL)-6, IL-8, tumor necrosis factor alpha (TNF-alpha), vascular endothelial growth factor (VEGF), and malondialdehyde (MDA) were measured by sandwich enzyme-linked immunosorbent assay and spectrophotometric assay and compared among the groups by independent sample t test and Mann-Whitney U-test.


RESULTS
Serum levels of the mediators in Group A (IL-6 [23.1 pg/mL], IL-8 [161.1 pg/mL], TNF-alpha [30.5 pg/mL], VEGF [455.9 pg/mL], MDA [10.7 microm]) were found to be significantly higher than those in Group B (IL-6 [12.2 pg/mL], IL-8 [48.4 pg/mL], TNF-alpha [18.6 pg/mL], VEGF [138.0 pg/mL], MDA [5.1 microm]).


CONCLUSIONS
This study demonstrated that increased serum levels of inflammatory mediators including IL-6, IL-8, TNF-alpha, VEGF, and MDA were possibly involved in the development of posterior uveitis in BD.
Ozdamar
Yasemin
Y
Department of Retinal and Uveal Diseases, Ankara Ulucanlar Eye Research Hospital, Ankara - Turkey. yasemin_oz@yahoo.com
Berker
Nilufer
N
Bahar
Gul
G
Soykan
Emel
E
Bicer
Tolga
T
Ozkan
Seyhan Sonar
SS
Karakaya
Jale
J
D000328:Adult; D001528:Behcet Syndrome / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D007378:Interleukins / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015866:Uveitis, Posterior / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D055815:Young Adult
D018836:Inflammation Mediators; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde