[Effect of proliferation, c-met and vascular endothelial growth factor expression in the Eca109/9706 cells by liposomal quercetin].
OBJECTIVE
To explore the effect of cell proliferation, c-met and vascular endothelial growth factor (VEGF) expression by liposomal quercetin in the Eca109/9706 cells induced by liposomal quercetin.


METHODS
The suppressing rate of cultured Eca109/9706 cells was detected by MTT assay. After 48-hour-exposure to liposomal quercetin, the expression and cellular localization of c-met and VEGF in Eca109/9706 cells were examined by using immunohistochemistry assay and Western blotting.


RESULTS
The suppressing rate of Eca109/9706 cells was in the order of LQ2 group > LQ1 group > nLQ group > control group (P < 0.05). The protein expression of c-met and VEGF was found in Eca109/9706 cells cytoplasma as well as in nuclei by using immunohistochemistry assay. The Immuno-reaction intensity of c-met and VEGF was in the order of control group > nLQ group > LQ1 group > LQ2 group (P <0.05). The western blotting showed the intensity of immunoblotting signals for c-met-IR and VEGF-IR was in the order of control group > nLQ group > LQ1 group > LQ2 group (P < 0.05).


CONCLUSIONS
The liposomal quercetin could suppress the proliferation of culture Eca109/9706 cells, which was about to be related with the suppression high expression of c-met and VEGF.
Pei
Yingxin
Y
Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. pyx@zzu.edu.cn
Du
Xiaoyan
X
Li
Jinping
J
Gao
Hanchang
H
Wang
Shasha
S
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008081:Liposomes; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D000970:Antineoplastic Agents; D008081:Liposomes; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D011794:Quercetin; D019859:Proto-Oncogene Proteins c-met