Antimicrobial activity of aminoreductone against Helicobacter pylori.
Anti-Helicobacter pylori (H. pylori) effects of aminoreductone (AR), a Maillard reaction product, were evaluated in this study. AR effectively inhibited the growth of all 24 strains (19 clinical isolates and 5 isogenic mutants) irrespective of susceptibility to antibiotics and clinical manifestation. The minimum inhibitory concentration (MIC) of AR ranged from 0.5 to 5 mM. A killing assay with multiples of MIC was performed, demonstrating that the killing activity of AR was significantly higher than that of its derived melanoidin, an inhibitor of H. pylori urease-gastric mucin adherence, formed in the final stage of the Maillard reaction. These significant effects of AR on H. pylori were observed even in acidic conditions (pH 3). At most, 25 mM AR effectively exhibited bactericidal activity in all strains. These results rise up the possibility that foods containing AR, such as milk and dairy products, are valuable sources for preventing colonization of H. pylori in the stomach and its associated tissue damages.
Trang
Vu Thu
VT
Faculty of Agriculture, Kochi University, Monobe B-200, Nankoku, Japan.
Takeuchi
Hiroaki
H
Kudo
Hayato
H
Aoki
Akitoshi
A
Katsuno
Shinya
S
Shimamura
Tomoko
T
Sugiura
Tetsuro
T
Ukeda
Hiroyuki
H
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; C017945:scorbamic acid; D001205:Ascorbic Acid