Resolution and enhancement in nanoantenna-based fluorescence microscopy.
Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the gold nanoparticle. We examine the interplay between fluorescence enhancement and spatial resolution and discuss the requirements for deciphering single molecules in a dense sample. Resolutions better than 20 nm and fluorescence enhancement up to 30 times are demonstrated experimentally. By accounting for the tip shaft and the sample interface in finite-difference time-domain calculations, we explain why the measured fluorescence enhancements are higher in the presence of an interface than the values predicted for a homogeneous environment.
Eghlidi
Hadi
H
Laboratory of Physical Chemistry and optETH, ETH Zurich, CH-8093 Zurich, Switzerland.
Lee
Kwang Geol
KG
Chen
Xue-Wen
XW
Götzinger
Stephan
S
Sandoghdar
Vahid
V
D003198:Computer Simulation; D003287:Contrast Media / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003287:Contrast Media; D006046:Gold