title: [Effect of all-trans retinoic acid deficiency on iron metabolism in rat primary hepatocyte].
abstract: OBJECTIVE
  To study the influence of atRA on iron metabolism in cultured primary rat hepatocyte.
  
  
  METHODS
  Rat primary hepatocytes were isolated by two-step in situ collagenase perfusion method by Seglen, and after that Cell viability was observed by 0.4% trypan blue. And then primary hepatocyte were treated into 6 wells plate with 0, 0.5, 1 and 50 micromol/L atRA and DMEM contained 10% fetal bovine serum. After 72h, IRP2 mRNA, TFR mRNA, Fnm RNA levels were measured by RT-PCR.
  
  
  RESULTS
  VA deficiency can decrease the viability and function. Moreover hepatic IRP2 mRNA and TFRmRNA levels were increased by VA deficiency, which diminishing expression of Fn mRNA.
  
  
  CONCLUSIONS
  vitamin A deficiency can change cellular iron metabolism by inducing IRP2-Fn-TFR pathway. AtRA supplementation inhibited the increase in IRP2 mRNA expression. Taken together, these results indicate that vitamin A deficiency can regulate iron metabolism by IRP2-TFR-Fn pathway.
authors.affiliation: Department of Food and Nutritional Health, College of Public Health, Harbin Medical University, Harbin 150081, China. lanlan8153@163.com
authors.forename: Lan
authors.initials: L
authors.lastname: Lan
authors.forename: Chaoxu
authors.initials: C
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Kebo
authors.initials: K
authors.lastname: Wang
authors.forename: Baosheng
authors.initials: B
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D035942:Iron Regulatory Protein 2 / Q000235:genetics / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014802:Vitamin A Deficiency / Q000503:physiopathology*
chemical_list: D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014212:Tretinoin; D007501:Iron; D035942:Iron Regulatory Protein 2