High-dynamic-range quantitative phase imaging with spectral domain phase microscopy.
Phase microscopy for high-dynamic-range quantitative phase-contrast imaging of a transparent phase object was demonstrated. Using a common path Fourier domain optical coherence tomography system, this technique is capable of displacement measurement with a sensitivity of 34 pm. The limitation of 2pi ambiguity restriction was overcome by the use of a phase retrieval approach performed in spectral domain. Two-dimensional quantitative phase imaging of human neonatal dermal keratinocyte cells was demonstrated to evaluate the performance of the system for cell imaging.
Zhang
Jun
J
Beckman Laser Institute and the Center for Biomedical Engineering, University of California, Irvine, Irvine, California 92612, USA. junzhang@uci.edu
Rao
Bin
B
Yu
Lingfeng
L
Chen
Zhongping
Z
D020405:Dermis / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000166:cytology; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D011336:Probability