title: OVO homologue-like 1 (Ovol1) transcription factor: a novel target of neurogenin-3 in rodent pancreas.
abstract: OBJECTIVE
  The basic helix-loop-helix transcription factor neurogenin-3 (NGN3) commits the fates of pancreatic progenitors to endocrine cell types, but knowledge of the mechanisms regulating the choice between proliferation and differentiation of these progenitors is limited.
  
  
  METHODS
  Using a chromatin immunoprecipitation cloning approach, we searched for direct targets of NGN3 and identified a zinc-finger transcription factor, OVO homologue-like 1 (OVOL1). Transactivation experiments were carried out to elucidate the functional role of NGN3 in Ovol1 gene expression. Embryonic and adult rodents pancreases were immunostained for OVOL1, Ki67 and NGN3.
  
  
  RESULTS
  We showed that NGN3 negatively regulates transcription of Ovol1 in an E-box-dependent fashion. The presence of either NGN3 or NEUROD1, but not MYOD, reduced endogenous Ovol1 mRNA. OVOL1 was detected in pancreatic tissue around embryonic day 15.5, after which OVOL1 levels dramatically increased. In embryonic pancreas, OVOL1 protein levels were low in NGN3(+) or Ki67(+) cells, but high in quiescent differentiated cells. OVOL1 presence was maintained in adult pancreas, where it was detected in islets, pancreatic ducts and some acinar cells. Additionally OVOL1 presence was lacking in proliferating ductules in regenerating pancreas and induced in cells as they began to acquire their differentiated phenotype.
  
  
  CONCLUSIONS
  The timing of OVOL1 appearance in pancreas and its increased levels in differentiated cells suggest that OVOL1 promotes the transition of cells from a proliferating, less-differentiated state to a quiescent more-differentiated state. We conclude that OVOL1, a downstream target of NGN3, may play an important role in regulating the balance between proliferation and differentiation of pancreatic cells.
authors.affiliation: Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA. amedeo.vetere@joslin.harvard.edu
authors.forename: A
authors.initials: A
authors.lastname: Vetere
authors.forename: W-C
authors.initials: WC
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Paroni
authors.forename: K
authors.initials: K
authors.lastname: Juhl
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.forename: W
authors.initials: W
authors.lastname: Nishimura
authors.forename: X
authors.initials: X
authors.lastname: Dai
authors.forename: S
authors.initials: S
authors.lastname: Bonner-Weir
authors.forename: A
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D049109:Cell Proliferation; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000473:pathology; D010180:Pancreatectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C407737:Neurog3 protein, mouse; C498696:Neurog3 protein, rat; C490318:Ovo1 protein, mouse