title: Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins.
abstract: Ubiquitination regulates not only the stability but the localization and activity of substrate proteins involved in a plethora of cellular processes. The Skp1-Cullin-F-box protein (SCF) complexes constitute a major family of ubiquitin protein ligases, in each member of which an F-box protein serves as the variable component responsible for substrate recognition, thereby defining the function of each complex. Here we studied whether the composition of F-box proteins in the SCF complexes is remodeled under different conditions. We exploited stable isotope labeling and MS for relative quantification of F-box proteins in the SCF complexes affinity-purified en masse from budding yeast cells at log and post-diauxic phases, and revealed an increment of Saf1, an F-box protein involved in entry into quiescence, during the diauxic shift. Similarly, we found that Met4 overexpression induces a specific increment of Met30, the F-box protein responsible for ubiquitination of Met4. These results illustrate a cellular response to environmental and genetic perturbations through remodeling of the SCF complex-mediated ubiquitination system. Compositional alteration of incorporated F-box proteins may redirect the activity of this system toward appropriate substrates to be ubiquitinated under individual conditions for the maintenance of cellular homeostasis.
authors.affiliation: Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kato
authors.forename: Keiji
authors.initials: K
authors.lastname: Kito
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ota
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
mesh_terms: D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D054875:Ubiquitination
chemical_list: D044783:F-Box Proteins; D012333:RNA, Messenger; D044843:SKP Cullin F-Box Protein Ligases