Identification of proteins differentially expressed between capillary endothelial cells of hepatocellular carcinoma and normal liver in an orthotopic rat tumor model using 2-D DIGE.
Hepatocellular carcinoma (HCC) is one of the deadliest cancers with few treatment options. It is a hypervascular tumor in which angiogenesis plays a critical role in its progression. Tumor capillary endothelial cells (TECs) in HCC are known to originate from liver sinusoid endothelial cells, which then go through a capillarization process to become morphologically as well as functionally different TECs. In this work, we investigated proteins differentially expressed between freshly isolated TECs and sinusoid endothelial cells from well-formed rat HCC using 2-D DIGE coupled with MALDI-TOF/TOF MS. Thirty-eight unique proteins were identified to be differentially expressed more than twofold between the two endothelial cell types. Amongst the differentially expressed proteins, two novel endothelial markers, EH domain-containing protein 3 and galectin-3, were confirmed by Western blot and immunohistochemistry in both rat and human HCC samples. We showed that EH domain-containing protein 3 is significantly down-regulated in TECs, but galectin-3 is up-regulated. We propose possible roles of these two proteins in tumor vessel development in HCC.
Jia
Jinghui
J
Department of Biological Sciences, National University of Singapore, 117543, Singapore.
Wang
Jingyu
J
Teh
Ming
M
Sun
Wei
W
Zhang
Jianhua
J
Kee
Irene
I
Chow
Pierce K-H
PK
Liang
Rosa Cynthia M-Y
RC
Chung
Maxey C M
MC
Ge
Ruowen
R
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis; D002469:Cell Separation; D048430:Cell Shape; D004195:Disease Models, Animal; D004589:Electrophoresis, Disc; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000737:chemistry*; D037502:Galectin 3 / Q000032:analysis; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008113:Liver Neoplasms / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology; D008297:Male; D020543:Proteome / Q000032:analysis*; D051381:Rats
D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D037502:Galectin 3; D020543:Proteome