Fabrication of cellular polycaprolactone films for cell culture.
Hierarchical cellular structures consisting of multilayers of ordered pores were created on a poly-epsilon-caprolactone (PCL) film by introducing a colloidal crystal mask/template in thermomechanical lithography. The surface characteristics were measured by scanning electron microscopy, atomic force microscopy, water contact angle and analyzed by Fourier Transform (FT). The resultant PCL films present a metastable superhydrophobicity. Their water contact angles were initially 150 degrees and then declined with time eventually to 115-120 degrees . In vitro culture of human fibroblasts found that the cells could spread on the cellular PCL surface as normal, but showed higher viability compared with the control cells on a flat substrate.
Wang
Bo
B
MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, PR China.
Mao
Zhengwei
Z
Meng
Xinan
X
Tong
Weijun
W
Gao
Changyou
C
D002448:Cell Adhesion; D018929:Cell Culture Techniques; D003102:Colloids / Q000737:chemistry; D005347:Fibroblasts / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties
D003102:Colloids; D011091:Polyesters; C016240:polycaprolactone