title: Scanning electron microscope imaging of bacteria based on DNA sequence.
abstract: OBJECTIVE
  To develop a scanning electron microscopic approach using in situ hybridization (SEM-ISH) for gaining both genetic and morphological information about target bacteria.
  
  
  RESULTS
  Target cells were hybridized with DNA-targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM-ISH enabled to detect low copy number target DNA sequences in individual cells.
  
  
  CONCLUSIONS
  SEM-ISH allowed the in situ detection of bacteria carrying a specific gene.
  
  
  CONCLUSIONS
  Combining morphological study with SEM and ISH techniques appears to be a valuable tool to understand the spatial distribution of target cells in complex microbial communities on various materials.
authors.affiliation: Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kenzaka
authors.forename: A
authors.initials: A
authors.lastname: Ishidoshiro
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: M
authors.initials: M
authors.lastname: Nasu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial