title: Quantitative optical spectroscopy can identify long-term local tumor control in irradiated murine head and neck xenografts.
abstract: Noninvasive and longitudinal monitoring of tumor oxygenation status using quantitative diffuse reflectance spectroscopy is used to test whether a final treatment outcome could be estimated from early optical signatures in a murine model of head and neck cancer when treated with radiation. Implanted tumors in the flank of 23 nude mice are exposed to 39 Gy of radiation, while 11 animals exposed to sham irradiation serve as controls. Diffuse optical reflectance is measured from the tumors at baseline (prior to irradiation) and then serially until 17 days posttreatment. The fastest and greatest increase in baseline-corrected blood oxygen saturation levels are observed from the animals that show complete tumor regression with no recurrence 90 days postirradiation, relative to both untreated and treated animals with local recurrences. These increases in saturation are observed starting 5 days posttreatment and last up to 17 days posttreatment. This preclinical study demonstrates that diffuse reflectance spectroscopy could provide a practical method far more effective than the growth delay assay to prognosticate treatment outcome in solid tumors and may hold significant translational promise.
authors.affiliation: Duke University, Department of Biomedical Engineering, 136 Hudson Hall, Box 90281, Durham, North Carolina 27708, USA. kvh1@duke.edu
authors.forename: Karthik
authors.initials: K
authors.lastname: Vishwanath
authors.forename: Daniel
authors.initials: D
authors.lastname: Klein
authors.forename: Kevin
authors.initials: K
authors.lastname: Chang
authors.forename: Thies
authors.initials: T
authors.lastname: Schroeder
authors.forename: Mark W
authors.initials: MW
authors.lastname: Dewhirst
authors.forename: Nimmi
authors.initials: N
authors.lastname: Ramanujam
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000532:radiotherapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor