title: Clonally expanded plasma cells in the cerebrospinal fluid of patients with central nervous system autoimmune demyelination produce "oligoclonal bands".
abstract: Clonally expanded plasma cells (cePC) and oligoclonal IgG (oligoclonal bands, OCB) in the cerebrospinal fluid (CSF) suggest an involvement of B cell mechanisms in autoimmune CNS demyelination. Due to their CSF-restricted occurrence, OCB are commonly believed to be the products of B cells inside the borders of the blood brain barrier. A comparison of CSF cell Ig transcriptomes and CSF-Ig proteomes recently demonstrated, that in multiple sclerosis patients CSF cells are the origin of CSF immunoglobulins. We expand these findings by applying anti-idiotypic antibodies to detect specific heavy chain CDR3 idiotopes of cePC-produced antibodies amongst OCB in the CSF of a patient each with MS and acute disseminated encephalomyelitis.
authors.affiliation: Dept. of Neurology, University Hospital Zürich, Frauenklinikstrasse 26, 8091 Zürich, Switzerland. Hans-Christian.vonBuedingen@ucsf.edu
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: von Büdingen
authors.forename: Monica
authors.initials: M
authors.lastname: Gulati
authors.forename: Sandra
authors.initials: S
authors.lastname: Kuenzle
authors.forename: Katja
authors.initials: K
authors.lastname: Fischer
authors.forename: Tobias A
authors.initials: TA
authors.lastname: Rupprecht
authors.forename: Norbert
authors.initials: N
authors.lastname: Goebels
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic; D020278:Demyelinating Autoimmune Diseases, CNS / Q000134:cerebrospinal fluid* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D043025:Oligoclonal Bands / Q000134:cerebrospinal fluid*; D010950:Plasma Cells / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000888:Antibodies, Anti-Idiotypic; D043025:Oligoclonal Bands