Demonstration of anti-tumor activity of oncolytic measles virus strains in a malignant pleural effusion breast cancer model.
Breast cancer is the second leading cause of malignant effusions in cancer patients. Pleural effusion indicates incurable disease with limited palliative treatment options and poor outcome. Here, we demonstrate the therapeutic efficacy of measles virus (MV) vaccine strain derivative against malignant pleural effusion in an MDA-MB-231 xenograft model of advanced breast cancer. Both systemic intravenous (i.v.) and intrapleural (t.t.) administered virus caused massive infection and syncytia formation in the pleural tumor deposits. Intrapleural administration of 1.5 x 10(6) plaque-forming units (PFU) total dose of MV significantly improved median survival by approximately 80% compared to the control animal group. Furthermore, we tested human dendritic cells as carriers for delivery of oncolytic MV infection to breast cancer pleural metastases. Carrier-delivered MV infection prevented accumulation of the pleural exudate and also significantly improved the survival of the treated mice. This is the first demonstration of the therapeutic potential of oncolytic virotherapy against malignant pleural effusions in a pre-clinical model of advanced breast cancer.
Iankov
Ianko D
ID
Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Msaouel
Pavlos
P
Allen
Cory
C
Federspiel
Mark J
MJ
Bulur
Peggy A
PA
Dietz
Allan B
AB
Gastineau
Dennis
D
Ikeda
Yasuhiro
Y
Ingle
James N
JN
Russell
Stephen J
SJ
Galanis
Evanthia
E
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008457:Measles / Q000150:complications / Q000276:immunology; D008458:Measles Vaccine / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D050130:Oncolytic Virotherapy; D016066:Pleural Effusion, Malignant / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014779:Virus Replication; D023041:Xenograft Model Antitumor Assays
D008458:Measles Vaccine