title: Improved biocompatibility of thrombo-resistant iron-polysaccharides multilayer coatings on nitinols.
abstract: Biocompatibility of two multilayer coatings of (Fe3+/Hep)10 and (Fe3+/DS/Fe3+/Hep)5 was comparatively analyzed with respect to protein adsorption, leukocyte adhesion and cell-material interaction. Both of them showed significantly high albumin-to-fibrinogen adsorption ratio, suggesting good biocompatibility. Furthermore, the (Fe3+/DS/Fe3+/Hep)5 coating was found to exhibit the lowest non-specific protein adsorption due to the incorporation of dextran sulfate. Compared with uncoated Nitinol surfaces, iron-polysaccharide multilayer coating presented no deformation of leukocytes, indicating no signs of inflammatory reactions. Cell growth, cell adhesion and cell metabolic activity were all in good condition, verifying both (Fe3+/Hep)10 and (Fe3+/DS/Fe3+/Hep)5 coatings had good cytocompatibility. Therefore, iron-polysaccharides multilayer coatings had greatly improved the biocompatibility of Nitinols.
authors.affiliation: Nanobiotechnology Division, State Key Laboratory of Urban Water Resources and Environment (SKLUWRE), School of Sciences, Harbin Institute of Technology, Harbin 15001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Yue
authors.forename: Zhengbao
authors.initials: Z
authors.lastname: Zha
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Dai
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000418:Albumins / Q000378:metabolism; D000497:Alloys / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008422:Materials Testing; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011134:Polysaccharides / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D034421:Sus scrofa; D013927:Thrombosis / Q000473:pathology*
chemical_list: D000418:Albumins; D000497:Alloys; D020099:Coated Materials, Biocompatible; D011134:Polysaccharides; C013616:nitinol; D005340:Fibrinogen; D007501:Iron