title: Parallel methods for expression and purification.
abstract: Protein properties are highly diverse, making parallel expression and purification a particular challenge. Parallel methods are typically used when a number derivatives of a target protein are desired or when multiple homologs are needed. A typical scenario involves target evaluation, cloning and mutagenesis of the target, expression screening, large-scale expression and purification, and analytical and biophysical testing of the resulting protein. This chapter describes some of the strategies and methods employed for parallel protein expression and purification.
authors.affiliation: Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008832:Microchemistry / Q000379:methods; D008954:Models, Biological; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins