title: TNF superfamily gene polymorphism as prognostic factor in early breast cancer.
abstract: OBJECTIVE
  Since apoptosis may play a role in the prognosis of breast cancer, the present study analyzed the polymorphisms of apoptosis-related genes and their impact on the survival of 240 patients with early invasive ductal breast cancer.
  
  
  METHODS
  The genomic DNA was extracted from paraffin-embedded tumor-free tissue or blood, and 12 single nucleotide polymorphisms (SNPs) of 11 apoptosis-related genes in the apoptosis pathway determined using a Sequenom MassARRAY system.
  
  
  RESULTS
  During the median follow-up of 53.4 (range 2.9-205.9) months, 37 relapses and 22 deaths occurred. Among the target polymorphisms, the tumor necrosis factor superfamily member 10 gene polymorphism (TNFSF10 rs1131532) in a recessive model of the T allele and prostaglandin-endoperoxide synthase 2 gene polymorphism (PTGS2 rs5275) in a dominant model of the C allele were associated with survival in a log-rank test. The TT genotype of TNFSF10 (rs1131532) was also significantly correlated with a lower disease-free, distant disease-free, and overall survival in a multivariate analysis (HR = 3.304, 4.757, and 6.459; P = 0.002, 0.001, and 0.009, respectively), while PTGS2 rs5275 was only associated with a higher distant disease-free survival (HR = 0.302; P = 0.041). No clinicopathologic difference was observed according to the genotypes of these two polymorphisms.
  
  
  CONCLUSIONS
  The TNFSF10 (rs1131532) polymorphism was identified as a possible prognostic factor of survival in patients with operated invasive breast cancer.
authors.affiliation: Department of Surgery, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Jung-Gu, Daegu, Korea.
authors.forename: Jin Hyang
authors.initials: JH
authors.lastname: Jung
authors.forename: Yee Soo
authors.initials: YS
authors.lastname: Chae
authors.forename: Joon Ho
authors.initials: JH
authors.lastname: Moon
authors.forename: Byung Woog
authors.initials: BW
authors.lastname: Kang
authors.forename: Jong Gwang
authors.initials: JG
authors.lastname: Kim
authors.forename: Sang Kyun
authors.initials: SK
authors.lastname: Sohn
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Park
authors.forename: Myung Hoon
authors.initials: MH
authors.lastname: Lee
authors.forename: Ho Yong
authors.initials: HY
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D044584:Carcinoma, Ductal / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C439524:TNF protein, human; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2