Influence of laser parameters on nanoparticle-induced membrane permeabilization.
Light-absorbing nanoparticles that are heated by short laser pulses can transiently increase membrane permeability. We evaluate the membrane permeability by flow cytometry assaying of propidium iodide and fluorescein isothiocyanate dextran (FITC-D) using different laser sources. The dependence of the transfection efficiency on laser parameters such as pulse duration, irradiant exposure, and irradiation mode is investigated. For nano- and also picosecond irradiation, we show a parameter range where a reliable membrane permeabilization is achieved for 10-kDa FITC-D. Fluorescent labeled antibodies are able to penetrate living cells that are permeabilized using these parameters. More than 50% of the cells are stained positive for a 150-kDa IgG antibody. These results suggest that the laser-induced permeabilization approach constitutes a promising tool for targeted delivery of larger exogenous molecules into living cells.
Yao
Cuiping
C
Xi'an Jiaotong University, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xianning xi Road 28, Xi'an, 710049, China.
Qu
Xiaochao
X
Zhang
Zhenxi
Z
Hüttmann
Gereon
G
Rahmanzadeh
Ramtin
R
D002460:Cell Line; D002462:Cell Membrane; D002463:Cell Membrane Permeability / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007834:Lasers; D008223:Lymphoma / Q000503:physiopathology*; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D011829:Radiation Dosage