Sodium choleate (NaCho) effects on Candida albicans: implications for its role as a gastrointestinal tract inhabitant.
Candida albicans at times resides in the intestinal tract, where it experiences exposure to bile salts suggesting a study of the effects of crude bile salts in the form of sodium choleate (NaCho) on C. albicans growth, expression of virulent phenotypes, and adaptation to physiological challenges in vitro. Growth and phenotype alteration was examined by challenging clinical isolates of C. albicans with a wide range of NaCho concentrations by using conventional microbial physiology methods. Our results showed that (1) NaCho did not inhibit growth of yeast cells, up to 40 mg/ml; (2) NaCho powerfully stimulated the hypha formation; (3) NaCho at 2.5 and 5 mg/ml significantly induced CDR1p and biofilm formation, but these effects decreased at higher NaCho concentrations; (4) loss of cell integrity with exposure to 56 degrees C for 15 min, was exacerbated by increasing levels of NaCho; (5) NaCho protected yeast from hydrogen peroxide damage in a dose-dependent manner; and (6) catalase activity was increased by NaCho exposure.
Vu
Bao
B
Drake University, Des Moines, IA, USA.
Essmann
Michael
M
Larsen
Bryan
B
D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D041981:Gastrointestinal Tract / Q000382:microbiology* / Q000502:physiology*; D006801:Humans; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D020358:Sodium Cholate / Q000494:pharmacology*; D013997:Time Factors
D000935:Antifungal Agents; D036341:Intercellular Signaling Peptides and Proteins; D020358:Sodium Cholate