title: Multiplexed imaging of therapeutic cells with multispectrally encoded magnetofluorescent nanocomposite emulsions.
abstract: Here, we describe the fabrication of multispectrally encoded nanoprobes, perfluorocarbon (PFC)/quantum dots (QDs) nanocomposite emulsions, which could provide both multispectral MR and multicolor optical imaging modalities. Our strategy exploited the combination of the multispectral MR properties of four different PFC materials and the multicolor emission properties of three different colored CdSe/ZnS QDs. The PFC/QDs nanocomposite emulsions were fabricated by exchanging hydrophobic ligands coated onto CdSe/ZnS QDs using 1H,1H,2H,2H-perfluorooctanethiol, which renders the QDs to be dispersible in the PFC liquids. To provide biocompatibility, the PFC liquids containing QDs were emulsified into aqueous solutions with the aid of phospholipids. The distinct (19)F-based MR images of PFC/QDs nanocomposite emulsions were obtained by selective excitation of the nanocomposite emulsions with magnetic resonance frequency of each PFC, while a specific fluorescence image of them could be selected using appropriate optical filters. The uptake of PFC/QDs nanocomposite emulsions was high in phagocytic cells such as macrophages (90.55%) and dendritic cells (85.34%), while it was low in nonphagocytic T cells (33%). We have also shown that the nanocomposite emulsions were successfully applied to differentially visualize immunotherapeutic cells (macrophages, dendritic cells, and T cells) in vivo. The PFC/QDs nanocomposite emulsions are expected to be a promising multimodality nanoprobe for the multiplexed detection and imaging of therapeutic cells both in vitro and in vivo.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea.
authors.forename: Yong Taik
authors.initials: YT
authors.lastname: Lim
authors.forename: Young-Woock
authors.initials: YW
authors.lastname: Noh
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Cho
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Han
authors.forename: Bang Sil
authors.initials: BS
authors.lastname: Choi
authors.forename: Jina
authors.initials: J
authors.lastname: Kwon
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Hong
authors.forename: Anisha
authors.initials: A
authors.lastname: Gokarna
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Cho
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D004655:Emulsions; D005456:Fluorescent Dyes / Q000737:chemistry*; D008280:Magnetics
chemical_list: D004655:Emulsions; D005456:Fluorescent Dyes