[MALDI-TOF mass spectrometry in the investigation of large high-molecular biological compounds].
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry has become, in the recent years, a tool of choice for analyses of biological polymers. The wide mass range, high accuracy, informativity and sensitivity make it a superior method for analysis of all kinds of high-molecular biological compounds including proteins, nucleic acids and lipids. MALDI-TOF-MS is particularly suitable for the identification of proteins by mass fingerprint or microsequencing. Therefore it has become an important technique of proteomics. Furthermore, the method allows making a detailed analysis of post-translational protein modifications, protein-protein and protein-nucleic acid interactions. Recently, the method was also successfully applied to nucleic acid sequencing as well as screening for mutations.
Porubl'ova
L V
LV
Rebriiev
A V
AV
Hromovyĭ
T Iu
TIu
Minia
I I
II
Obolens'ka
M Iu
MIu
D008055:Lipids / Q000737:chemistry*; D008970:Molecular Weight; D009696:Nucleic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins