title: Fitness study of the RDRio lineage and Latin American-Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil.
abstract: RD(Rio) is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) family. LAM has been found worldwide but is more predominant in South America. The aim of this study was to assess the presence of the RD(Rio) lineage and LAM family in the city of Rio Grande, Brazil, and to investigate the fitness of these strains based on determination of their growth rate. Fifty clinical isolates of M. tuberculosis were genotyped and 43 different patterns were found by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats. The predominant genotypes belonged to the LAM family (54% of the strains) followed by clade T (22%) and Haarlem (16%). The RD(Rio) lineage represented 38% of the total strains and 70.4% of the LAM strains found in this study. Strains belonging to the LAM family showed a fitness advantage when comparing their rate of growth with that of non-LAM strains, but a significant difference between RD(Rio) and non-RD(Rio) strains was not confirmed.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. avongroll@itg.be
authors.forename: Andrea
authors.initials: A
authors.lastname: Von Groll
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.forename: Carolina
authors.initials: C
authors.lastname: Felix
authors.forename: Pedro Fernandes Sanmartin
authors.initials: PF
authors.lastname: Prata
authors.forename: Günther
authors.initials: G
authors.lastname: Honscha
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
authors.forename: Peter
authors.initials: P
authors.lastname: Vandame
authors.forename: Pedro Eduardo Almeida
authors.initials: PE
authors.lastname: da Silva
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D005260:Female; D056084:Genetic Fitness; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007843:Latin America; D008297:Male; D019083:Mediterranean Region; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult
chemical_list: D003470:Culture Media; D009841:Oligonucleotides