title: Interaction studies between human alpha-tocopherol transfer protein and nitric oxide donor tocopherol analogues with LDL-protective activity.
abstract: Nitric oxide-releasing alpha-tocopherol mimetics with LDL-protective activity were designed to maintain the tocopherol substructure necessary for its biochemical recognition by alpha-tocopherol transfer protein. In order to study the molecular interactions to alpha-TTP, theoretical binding studies by means of docking techniques and experimental binding assays, using a fluorescent probe, were performed. Furoxanyl-tocopherol-hybrid analogs 7 and 9 have the best ability to bind to alpha-TTP suggesting that they could be incorporated to LDL in vivo to further release nitric oxide and prevent oxidative modifications.
authors.affiliation: Departamento de Química Orgánica, Facultad de Química-Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. vlopez@fq.edu.uy
authors.forename: Gloria V
authors.initials: GV
authors.lastname: López
authors.forename: Luis E
authors.initials: LE
authors.lastname: Gómez
authors.forename: Nuria
authors.initials: N
authors.lastname: Campillo
authors.forename: Juan A
authors.initials: JA
authors.lastname: Páez
authors.forename: Kaleigh
authors.initials: K
authors.lastname: Giles
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Atkinson
authors.forename: Mercedes
authors.initials: M
authors.lastname: González
authors.forename: Homero
authors.initials: H
authors.lastname: Rubbo
authors.forename: Hugo
authors.initials: H
authors.lastname: Cerecetto
mesh_terms: D019943:Amino Acid Substitution; D000975:Antioxidants / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D024505:Tocopherols / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*; D024502:alpha-Tocopherol
chemical_list: D000975:Antioxidants; D008078:Cholesterol, LDL; D020030:Nitric Oxide Donors; D014810:Vitamin E; D009569:Nitric Oxide; D024502:alpha-Tocopherol; D024505:Tocopherols