II: Effects of a dopamine receptor antagonist on fathead minnow dominance behavior and ovarian gene expression in the fathead minnow and zebrafish.
Neurotransmitters such as dopamine play an important role in reproductive behaviors and signaling. Neuroendocrine-active chemicals in the environment have potential to interfere with and/or alter these processes. A companion study with the dopamine 2 receptor antagonist, haloperidol, found no evidence of a direct effect of the chemical on fish reproduction. This study considered haloperidol's potential effects on behavior and ovarian gene expression. Male fathead minnows exposed to 50 microg haloperidol/L for 96 h were found to be significantly more dominant than control males. In terms of molecular signaling, investigated using oligonucleotide microarrays, there was little similarity in the identity and functions of genes differentially expressed in the ovaries of fathead minnows (Pimephales promelas) versus zebrafish (Danio rerio) exposed under the same conditions. Results suggest that non-lethal concentrations of haloperidol do not induce ovarian molecular responses that could serve as biomarkers of exposure to D2R antagonists, but may impact behavior.
Villeneuve
Daniel L
DL
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, 6201 Congdon Blvd., Duluth, MN 55804, USA. villeneuve.dan@epa.gov
Garcia-Reyero
Natàlia
N
Martinović
Dalma
D
Mueller
Nathaniel D
ND
Cavallin
Jenna E
JE
Durhan
Elizabeth J
EJ
Makynen
Elizabeth A
EA
Jensen
Kathleen M
KM
Kahl
Michael D
MD
Blake
Lindsey S
LS
Perkins
Edward J
EJ
Ankley
Gerald T
GT
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003530:Cyprinidae / Q000254:growth & development; D018492:Dopamine Antagonists / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006220:Haloperidol / Q000633:toxicity*; D008297:Male; D010053:Ovary / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development
D018492:Dopamine Antagonists; D014874:Water Pollutants, Chemical; D006220:Haloperidol