title: Use of a baculovirus-expressed structural protein for the detection of antibodies to foot-and-mouth disease virus type A by a blocking enzyme-linked immunosorbent assay.
abstract: A blocking enzyme-linked immunosorbent assay (ELISA) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with a cutoff of 53% inhibition. Its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking ELISA, indicating its potential as an alternative assay.
authors.affiliation: National Veterinary Research and Quarantine Service, Anyang-6-dong, Anyang, Gyeonggi 430-824, South Korea. koyj@nvrqs.go.kr
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Ko
authors.forename: Hyang-Sim
authors.initials: HS
authors.lastname: Lee
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Jeoung
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Heo
authors.forename: Hyo-Rim
authors.initials: HR
authors.lastname: Ko
authors.forename: Byung-Sik
authors.initials: BS
authors.lastname: Chang
authors.forename: Hoo-Don
authors.initials: HD
authors.lastname: Joo
authors.forename: U
authors.initials: U
authors.lastname: Gerelmaa
authors.forename: B
authors.initials: B
authors.lastname: Dashzeveg
authors.forename: S
authors.initials: S
authors.lastname: Tserendorj
authors.forename: R
authors.initials: R
authors.lastname: Sodnomdarjaa
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.forename: Sang-Gi
authors.initials: SG
authors.lastname: Paik
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D005822:Genetic Vectors; D006041:Goats; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins