Contrasting genetic structures of two parasitic nematodes, determined on the basis of neutral microsatellite markers and selected anthelmintic resistance markers.
For the first time, the neutral genetic relatedness of natural populations of Trichostrongylid nematodes was investigated in relation to polymorphism of the beta-tubulin gene, which is selected for anthelminthic treatments. The aim of the study was to assess the contribution of several evolutionary processes: migration and genetic drift by neutral genetic markers and selection by anthelminthic treatments on the presence of resistance alleles at beta-tubulin. We studied two nematode species (Teladorsagia circumcincta and Haemonchus contortus) common in temperate climatic zones; these species are characterized by contrasting life history traits. We studied 10 isolated populations of goat nematode parasites: no infected adult goat had been exchanged after the herds were established. Beta-tubulin polymorphism was similar in these two species. One and two beta-tubulin alleles from T. circumcincta and H. contortus respectively were shared by several populations. Most of the beta-tubulin alleles were 'private' alleles. No recombination between alleles was detected in BZ-resistant alleles from T. circumcincta and H. contortus. The T. circumcincta populations have not diverged much since their isolation (F(ST) <0.08), whereas H. contortus displayed marked local genetic differentiation (F(ST) ranging from 0.08 to 0.18). These findings suggest that there are severe bottlenecks in the H. contortus populations, possibly because of their reduced abundance during unfavourable periods and their high reproductive rate, which allows the species to persist even after severe population reduction. Overall, the data reported contradict the hypothesis of the origin of beta-tubulin resistance alleles in these populations from a single mutational event, but two other hypotheses (recurrent mutation generating new alleles in isolated populations and the introduction of existing alleles) emerge as equally likely.
Silvestre
A
A
INRA, UR Infectiologie Animale et Santé Publique, Nouzilly, France. anne.silvestre@tours.inra.fr
Sauve
C
C
Cortet
J
J
Cabaret
J
J
D000483:Alleles; D000818:Animals; D018509:DNA, Helminth / Q000235:genetics; D005602:France; D005819:Genetic Markers; D005828:Genetics, Population; D006041:Goats / Q000469:parasitology; D018895:Microsatellite Repeats; D008957:Models, Genetic; D009154:Mutation; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014251:Trichostrongyloidea / Q000235:genetics*; D014404:Tubulin / Q000235:genetics
D018509:DNA, Helminth; D005819:Genetic Markers; D014404:Tubulin