Rapid detection of Infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification assay.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of Infectious bursal disease virus (IBDV). The RT-LAMP assay used a set of 4 primers to amplify the viral protein 2 gene of IBDV for the detection of IBDV, showing not only high efficiency but also analytic specificity. The data demonstrated that the RT-LAMP assay detected 30 different IBDV isolates, had no cross-reaction with 3 other avian viruses (Infectious bronchitis virus, Newcastle disease virus, and Avian influenza virus), and obtained a 95.45% sensitivity in 22 positive clinical samples in reference to virus isolation. Therefore, this rapid, specific, sensitive, and convenient RT-LAMP assay could be applicable to the identification of IBDV in less-equipped laboratories as well as in the field.
Xue
Chunyi
C
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
Zhang
Yun
Y
Zhou
Qingfeng
Q
Xu
Cong
C
Li
Xiaoming
X
Cao
Yongchang
Y
D000818:Animals; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002645:Chickens; D017931:DNA Primers; D005784:Gene Amplification; D005814:Genes, Viral; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification*; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D017931:DNA Primers