title: Comparison of microleakage in human and bovine substrates using confocal microscopy.
abstract: Microleakage in human and bovine teeth was compared. Cavities were prepared on the buccal surface in 20 human and 20 bovine teeth (3 mm x 2 mm in depth). The teeth were divided into 4 groups (n = 10) according to the substrate and adhesive (CLEARFIL SE Bond-CF or Scotchbond 1-SB1). Resin composite (Wave) was applied in two increments, each cured for 30 sec. Specimens were stored in 100% relative humidity at 37 degrees C for 24 hr and submitted to 1,000 thermal cycles, followed by immersion in 0.6% aqueous rhodamine for 48 hr. Specimens were rinsed and sectioned at the center. Microleakage length was measured and the score recorded using the following scale: 0-none, 1-up to enamel junction, 2-up to pulp wall, 3-in pulp wall, 4-beneath pulp wall. Percentage of leakage penetration into the cavity was submitted to an ANOVA and Tukey's test (5%) and the scores submitted to the Kruskal-Wallis and Dunn's multiple comparison tests (5%). When bovine teeth were used, SB1 (87.76%) showed a statistically higher penetration mean than CF (66.22%). When human teeth were used, no difference was found between SB1 (47.35%) and CF (36.01%). When scores were analyzed, SB1 showed no difference to CF. The differences found should be taken into consideration when evaluating adhesive microleakage using bovine teeth.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of North Parana, Londrina, PR, Brazil. baenalopes@yahoo.com.br
authors.forename: Murilo Baena
authors.initials: MB
authors.lastname: Lopes
authors.forename: Simonides
authors.initials: S
authors.lastname: Consani
authors.forename: Alcides
authors.initials: A
authors.lastname: Gonini-Júnior
authors.forename: Sandra Kiss
authors.initials: SK
authors.lastname: Moura
authors.forename: John Francis
authors.initials: JF
authors.lastname: McCabe
mesh_terms: D000818:Animals; D002417:Cattle; D003188:Composite Resins; D003763:Dental Leakage; D003793:Dental Restoration, Permanent / Q000379:methods*; D017220:Dentin-Bonding Agents; D006801:Humans; D018613:Microscopy, Confocal; D019279:Resin Cements
chemical_list: C438313:Clearfil SE Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C041330:Scotchbond