title: Monolithic silica capillary columns with immobilized cellulose tris(3,5-dimethylphenylcarbamate) for enantiomer separations in CEC.
abstract: Two types of monolithic silica capillary columns with an immobilized cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) selector were prepared for enantiomer separations in CEC. The monolithic columns were prepared by a sol-gel process in fused-silica capillaries. CDMPC was then either immobilized on a silica monolith through an intermolecular polycondensation of the cellulose derivative containing a triethoxysilyl group, or on a vinylized silica monolith through radical copolymerization of the cellulose derivative, which also contained a vinyl group. IR spectra confirmed the successful immobilization of CDMPC on both columns. Eleven chiral compounds were used to evaluate the enantioselectivity on both column types. Results indicated that the columns obtained via polycondensation had higher separation ability than those obtained via radical polymerization, and that they showed satisfactory run-to-run repeatability and stability. These new techniques thus provide strategies for preparing immobilized polysaccharide-based chiral silica monolithic capillary columns for chiral separations by means of CEC.
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel-VUB, Brussels, Belgium.
authors.forename: Chiyang
authors.initials: C
authors.lastname: He
authors.forename: Ans
authors.initials: A
authors.lastname: Hendrickx
authors.forename: Debby
authors.initials: D
authors.lastname: Mangelings
authors.forename: Johanna
authors.initials: J
authors.lastname: Smeyers-Verbeke
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004355:Drug Stability; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D048448:Phenylcarbamates / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; D011108:Polymers; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D012822:Silicon Dioxide; D002482:Cellulose