title: Antibodies to endogenous tear protein in normal human tears.
abstract: OBJECTIVE
  To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears.
  
  
  METHODS
  Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins.
  
  
  RESULTS
  Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins.
  
  
  CONCLUSIONS
  Normal human ocular tears contain antibodies to endogenous tear protein.
authors.affiliation: Department of Ophthalmology, HealthPartners Medical Group and Clinics, St Paul, Minnesota, USA. susann.g.remington@healthpartners.com
authors.forename: Susann G
authors.initials: SG
authors.lastname: Remington
authors.forename: Jean M
authors.initials: JM
authors.lastname: Crow
authors.forename: J Daniel
authors.initials: JD
authors.lastname: Nelson
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000276:immunology*; D054835:Lipocalin 1 / Q000276:immunology*; D009113:Muramidase / Q000276:immunology*; D013666:Tears / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D054835:Lipocalin 1; D009113:Muramidase; D007781:Lactoferrin