Structural and immunochemical studies of neutral exopolysaccharide produced by Lactobacillus johnsonii 142.
This paper describes the structure of neutral exopolysaccharide (EPS) produced by Lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (IBD). Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, NOESY, and (1)H,(13)C HSQC experiments revealed that the repeating unit of the EPS is a pentasaccharide: -->3)-alpha-D-Galp-(1-->3)-beta-d-Glcp-(1-->5)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->3)-alpha-D-Galp-(1--> The rabbit antiserum raised against whole cells of L. johnsonii 142 reacted with homologous EPS, and cross-reacted with exopolysaccharide from Lactobacillus animalis/murinus 148 isolated also from mice with IBD, but not reacted with EPS of L. johnsonii 151 from healthy mice.
Górska
Sabina
S
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Jachymek
Wojciech
W
Rybka
Jacek
J
Strus
Magdalena
M
Heczko
Piotr B
PB
Gamian
Andrzej
A
D000818:Animals; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D015212:Inflammatory Bowel Diseases / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D007778:Lactobacillus / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial