Synaptic currents and transmitter responses in human NT2 neurons differentiated in aggregate culture.
Postmitotic neurons were generated from the human NT2 teratocarcinoma cell line in a novel cell aggregate differentiation procedure. The NT2 model neurons express punctate immunoreactivity for synapsin and for cell markers related to GABAergic and glutamatergic neurotransmission. Using the outside-out patch-clamp configuration, we characterized the kinetics of currents elicited by a rapid application of the amino acid neurotransmitters. Moreover, we detected spontaneous postsynaptic currents in glia free cell cultures that may result from the firing activity of glutamatergic and GABAergic NT2 neurons. These cultured spontaneously active networks may be a useful tool to analyze factors that modulate the formation and efficacy of synapses between human neurons.
Podrygajlo
Grzegorz
G
Div. of Cell Biology, Institute of Physiology, University of Veterinary Medicine Hannover, Hannover, Germany.
Song
Yunping
Y
Schlesinger
Friedrich
F
Krampfl
Klaus
K
Bicker
Gerd
G
D002449:Cell Aggregation; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D019706:Excitatory Postsynaptic Potentials; D018698:Glutamic Acid / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D053444:Inhibitory Postsynaptic Potentials; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology*; D018408:Patch-Clamp Techniques; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid