Vibration model for hypoxic type of cell metabolism evaluated on rabbit cardiomyocytes.
Activity of the energy production system in rabbits myocardium was examined under various conditions of whole-body vibration. The energy-dependent response of native mitochondria from rabbit heart was studied polarographically using a Clark closed membrane electrode. In was established that the energy production system in rabbit myocardium was involved in organism's response to whole-body vibration. Functional changes in myocardial mitochondria were shown to depend on the mode of whole-body vibration (frequency and duration). It manifested in an imbalance between functional activity of FAD- and NAD-dependent components of the respiratory chain. The increase in the frequency and duration of vibration was accompanied by dysfunction of the energy production system in cardiomyocytes. These changes manifested in activation of succinic acid oxidation and inhibition of NAD-dependent components of the mitochondrial respiratory chain. Vibration due to systemic dysregulatory influence can be used as a model for studies of both vibration phenomenon realized at the level of the energy production system in organs and tissues and vibroprotective properties of medicinal products.
Vorobieva
V V
VV
Department of Pharmacology, S. M. Kirov Military Medical Academy, St. Petersburg.
Shabanov
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PD
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D004566:Electrodes; D004734:Energy Metabolism / Q000502:physiology; D008929:Mitochondria, Heart / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011817:Rabbits; D014732:Vibration