[Effects of p,p'-DDE on expression of inhibin B in sertoli cells of rats].
OBJECTIVE
To study the effects of p,p'-DDE on the expression of inhibin B in sertoli cells of rats.


METHODS
Sertoli cells were separated and cultured from testicular tissue of rats, and ensured the dose of test materials by MTT test, and RT-PCR were measured on the changes of inhibin B expression.


RESULTS
The results indicated that there was high difference in middle and high dose groups in comparison with control group (P < 0.05), and there were statistical significance in comparison with low dose group, and the gray scale of inhibin B decreased with the increase dose of p,p'-DDE.


CONCLUSIONS
The results indicated that p,p'-DDE could inhibit the expression of inhibin B.
Wang
Chong
C
Institute for Environmental Health and Related Product Safety, China CDC, Beijing 100021, China. wangchongfly@163.com
Liu
Hongkai
H
Zhang
Hongwei
H
Yang
Kedi
K
D000818:Animals; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000378:metabolism*
D007306:Insecticides; D012333:RNA, Messenger; C422364:inhibin B; D003633:Dichlorodiphenyl Dichloroethylene; D007265:Inhibins