[Effects of total flavonoids from Rhizoma Drynariae medicated serum on proliferation, differentiation, cell cycle and apoptosis of osteoblasts in vitro].
OBJECTIVE
To observe the effects of total flavonoids from Rhizoma Drynariae medicated serum on cell proliferation, differentiation, cell cycle and apoptosis of rats' osteoblasts cultured in vitro.


METHODS
The osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP, PI and Annexin V/PI methods were used to observe the proliferation, activity of alkaline phosphatase (ALP), every stage cells in the cell cycle, and the ratio of apoptosis cells by different concentrations of total flavonoids from Rhizoma Drynariae medicated serum at different time points.


RESULTS
Different concentrations of total flavonoids from Rhizoma Drynariae medicated serum could significantly enhance the cell proliferation rate and ALP activity (P < 0.05), more cells in S stage and less cells apoptosised than that of the untreated osteoblasts (P < 0.05).


CONCLUSIONS
Total flavonoids from Rhizoma Drynariae medicated serum could promote the proliferation, differentiation, increase S stage cells, and reduce the ratio of apoptosis cells, which reveals that they have anti-osteoporosis activity.
Zhang
Jun
J
Department of Orthopedics, Second Hospital Affilicated to Medical College of Xi'an Jiaotong University, Xi'an 710004, China. zjxjtu2001@stu.xtu.edu.cn
Li
Hao-Peng
HP
Yang
Ping-Lin
PL
Liu
Yi-Heng
YH
Yang
Bao-Hui
BH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010024:Osteoporosis / Q000517:prevention & control; D029621:Polypodiaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005419:Flavonoids; D000469:Alkaline Phosphatase