Single-nucleotide polymorphism (SNP) analysis to associate cancer risk.
Identification of hereditary factors that predispose to cancer allows targeted cancer screening and better quantification of environmental risk factors. The ability to identify which single nucleotide polymorphisms (SNPs) are associated with cancer or segregate with disease in families allows high-risk loci to be identified. In this chapter, two platforms for analysing SNPs are discussed, the Affymetrix and Illumina systems. Application of both platforms requires the same principles of good laboratory practice but there are important differences in materials and methods, which will be discussed.
Earl
Julie
J
Division of Surgery and Oncology, University of Liverpool, Liverpool, UK.
Greenhalf
William
W
D004247:DNA / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008967:Molecular Biology / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D012306:Risk
D004247:DNA