An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers.
Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.
Erturk
Y
Y
Hamza Polat Vocational School, Ataturk University, Ispir-Erzurum, Turkey.
Ercisli
S
S
Maghradze
D
D
Orhan
E
E
Agar
G
G
D001483:Base Sequence; D017931:DNA Primers; D014644:Genetic Variation; D027861:Prunus / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers