title: PCR detection of Histoplasma capsulatum var. capsulatum in whole blood of a renal transplant patient with disseminated histoplasmosis.
abstract: We report the identification of Histoplasma capsulatum var. capsulatum from whole blood in a renal transplant patient with disseminated histoplasmosis using colorimetric microtiter-plate PCR. This modality demonstrated utility in reaching a definitive diagnosis in a timely manner. Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis.
authors.affiliation: Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Julianne
authors.initials: J
authors.lastname: Qualtieri
authors.forename: Charles W
authors.initials: CW
authors.lastname: Stratton
authors.forename: David R
authors.initials: DR
authors.lastname: Head
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
mesh_terms: D000328:Adult; D005260:Female; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000097:blood* / Q000382:microbiology*; D006801:Humans; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling