title: Gel free analysis of the proteome of intracellular Leishmania mexicana.
abstract: Investigating the proteome of intracellular Leishmania amastigotes has recently become possible due to the exploitation of fluorescence activated intracellular parasite sorting. Here, we employed this technology in combination with gel free analysis to greatly improve proteome coverage and suggest proteins putatively secreted by the parasites. In total, 1764 proteins were identified of which 741 had not been reported before. Protein abundance indices were calculated to rank individual proteins according to their abundance in vivo. Using the LeishCyc resource, an overview of metabolically relevant proteins was produced that integrated protein abundance data. Bioinformatic analysis identified 143 proteins possibly secreted by L. mexicana amastigotes, half of which have no known function. The data provide a useful resource, e.g. for modelling metabolic flux or selecting novel vaccine antigens.
authors.affiliation: Institute of Immunology and Infection Research, The University of Edinburgh, Edinburgh, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Paape
authors.forename: Martin E
authors.initials: ME
authors.lastname: Barrios-Llerena
authors.forename: Thierry
authors.initials: T
authors.lastname: Le Bihan
authors.forename: Logan
authors.initials: L
authors.lastname: Mackay
authors.forename: Toni
authors.initials: T
authors.lastname: Aebischer
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D007894:Leishmania mexicana / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D051379:Mice; D008808:Mice, Inbred CBA; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D020543:Proteome; D015800:Protozoan Proteins