Chemical composition of the Lippia origanoides essential oils and their antigenotoxicity against bleomycin-induced DNA damage.
The present work evaluated the chemical composition of the essential oils (EO) obtained from Lippia origanoides and their DNA protective effect against bleomycin-induced genotoxicity. L. origanoides EO chemical composition was determined by gas chromatography-mass spectrometry (GC-MS). The major compounds of the L. origanoides EOs were thymol (34-58%) and carvacrol (26%). The antigenotoxic effects of the EOs, major compounds and standard compound (epigallocatechin gallate) were assayed in co-incubation procedures using the SOS chromotest in Escherichia coli. Both EOs and their major compounds protected bacterial cells against bleomycin-induced genotoxicity indicating that these two compounds were principally responsible for the antigenotoxicity detected in the oils. Thymol and carvacrol antigenotoxicity was lower than those observed with epigallocatechin gallate. The results were discussed in relation to the chemopreventive potential of L. origanoides EOs and their major components, carvacrol and thymol.
Vicuña
Gloria Carolina
GC
Biology School, Faculty of Sciences, Industrial University of Santander, Bucaramanga, Colombia.
Stashenko
Elena E
EE
Fuentes
Jorge Luis
JL
D016587:Antimutagenic Agents / Q000032:analysis / Q000494:pharmacology*; D001761:Bleomycin; D002392:Catechin / Q000031:analogs & derivatives; D018929:Cell Culture Techniques; D000081007:Cymenes; D004249:DNA Damage / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D035264:Flowers; D008401:Gas Chromatography-Mass Spectrometry; D032411:Lippia / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis / Q000494:pharmacology*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D013943:Thymol / Q000032:analysis / Q000494:pharmacology
D016587:Antimutagenic Agents; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D001761:Bleomycin; D013943:Thymol; D002392:Catechin; C073316:carvacrol; C045651:epigallocatechin gallate