Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase.
A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase.
Chitta
K R
KR
Department of Chemistry, P. O. Box 210172, Cincinnati, OH 45221-0172, USA.
Van Meter
D S
DS
Stalcup
A M
AM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry
D010455:Peptides; D012117:Resins, Synthetic