Oxidative stress in liver and red blood cells in acute lindane toxicity in rats.
The aim of our study was to determine the role and dynamics of oxidative and nitrosative stress, as well as superoxide dismutase (SOD) and catalase activity in the hepatocytes and erythrocytes in early phase of acute lindane intoxication. Male Wistar rats were divided into groups: control, dimethylsulfoxide and lindane-treated groups (L, 8 mg/kg, intraperitoneally). Animals were sacrificed 0.5 and 4 hours after treatment (L(0.5) and L(4) groups, respectively). Oxidative and nitrosative stress parameters and antioxidant enzymes were determined spectrophotometrically. Liver and plasma thiobarbituric acid reactive substances (TBARS) concentration were significantly increased 0.5 after lindane administration (p < .01), with subsequent additional rise within 4 hours (p < .01), while plasma nitrite + nitrate level was significantly higher only 4 hours after lindane treatment. Total liver SOD activity was significantly increased in L(4) group in comparison with control group (p < .01). In conclusion, oxidative and nitrosative stress play an important role in early phase of acute lindane hepatotoxicity. Antioxidant capacity of hepatocytes is partly increased, due to an adaptive increase in SOD activity.
Radosavljević
T
T
Department of Pathophysiology, School of Medicine, University of Belgrade, Dr Subotića 9, 11000 Belgrade, Serbia. tanjamm@med.bg.ac.yu
Mladenović
D
D
Jakovljević
V
V
Vucvić
D
D
Rasć-Marković
A
A
Hrncić
D
D
Djuric
D
D
Stanojlović
O
O
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D026361:Reactive Nitrogen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D007306:Insecticides; D009566:Nitrates; D009573:Nitrites; D026361:Reactive Nitrogen Species; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D001556:Hexachlorocyclohexane; D013482:Superoxide Dismutase