title: Cross-reaction between proteins of Larrea divaricata Cav. (jarilla) and proteins of Gram-negative bacteria.
abstract: Larrea divaricata is an abundant plant of northwest of Argentina used to treat different pathologies. We aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and whole cell-bacterial proteins (W-CBP) of Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Klebsiella pneumoniae using a mouse anti-JPCE serum. Protein profiles of JPCE and W-CBP were analyzed. For JPCE, 18 bands were observed in a 20-176 kDa range. Levels of IgG against JPCE and W-CBP were determined. Bacterial proteins showed a strong reaction with the anti-JPCE serum. Plant proteins could be used as immune stimulants.
authors.affiliation: Microbiology Unit, Department of Biochemistry and Biological Sciences, Faculty of Chemistry and Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
authors.forename: María Aída Mattar
authors.initials: MA
authors.lastname: de Anaya
authors.forename: Roberto
authors.initials: R
authors.lastname: Davicino
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Casali
authors.forename: Silvia
authors.initials: S
authors.lastname: Correa
authors.forename: Blas
authors.initials: B
authors.lastname: Micalizzi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000276:immunology; D032442:Larrea / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000276:immunology / Q000378:metabolism; D018515:Plant Leaves / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011511:Proteus / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D000939:Epitopes; D010936:Plant Extracts; D010940:Plant Proteins