Laboratory Maintenance of Bordetella pertussis.
The causative agent of the respiratory disease whooping cough, Bordetella pertussis, is a nutritionally fastidious microorganism but can be grown with relative ease in research laboratories. Stainer-Scholte synthetic broth medium and Bordet-Gengou blood agar both support growth of B. pertussis and are commonly used. B. pertussis prefers aerobic conditions and a temperature range of 35 degrees to 37 degrees C. Appropriate laboratory safety protocols are required to prevent the generation of aerosols, which could potentially spread this highly infectious agent.
Hulbert
Robin R
RR
California Polytechnic State University, San Luis Obispo, California, USA.
Cotter
Peggy A
PA
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D001886:Bordetella pertussis / Q000254:growth & development* / Q000302:isolation & purification; D003264:Containment of Biohazards; D003470:Culture Media / Q000737:chemistry; D005612:Freeze Drying / Q000379:methods; D006801:Humans; D014917:Whooping Cough / Q000382:microbiology
D003470:Culture Media