A GC-magnetic sector MS method for identification and quantification of fatty acids in ewe milk by different acquisition modes.
A general method for qualitative and quantitative determination of fatty acids (FAs) using GC-MS was developed and tested on ewe milk. A total number of 38 poly unsaturated FAs, monounsaturated FAs and saturated FAs, from C6:0 to C24:1, were used in a comparative study of scan, reconstructed ion chromatogram and SIM. Fatty acid methyl esters in standard solutions as well as in milk from ewe were analyzed by these techniques, using a sector instrument. Instrument precision, linearity, LOD and LOQ, as well as calibration behavior and response factors were investigated for each approach. The quantitative results obtained by each technique were compared. All techniques had values for LOD and LOQ in the ng/mL region.
Devle
Hanne
H
Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, As, Norway.
Rukke
Elling-Olav
EO
Naess-Andresen
Carl Fredrik
CF
Ekeberg
Dag
D
D000818:Animals; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D012756:Sheep
D005227:Fatty Acids