Predictive value of early serum beta-hCG levels after single blastocyst transfer.
OBJECTIVE
Analysis of serum beta-human chorionic gonadotropin (beta-hCG) levels on standardized days after oocyte retrieval (OR) following single blastocyst transfer to predict pregnancy outcome after assisted reproductive technology.


METHODS
Retrospective study.


METHODS
Private fertility center.


METHODS
A total of 230 women with an intact intrauterine pregnancy 21 days after OR and a vital term delivery (n = 191) or a miscarriage (< 10+0 gestational weeks) (n = 39) in the course of the pregnancy following single blastocyst transfer between 1999 and 2008.


METHODS
Blood was sampled 11, 14 and partly 21 days after OR. The serum concentration of total beta-hCG was measured by an automated quantitative immunoassay. Receiver operating characteristic curves were generated to calculate sensitivity and specificity.


METHODS
A total of 509 beta-hCG measurements.


RESULTS
The mean beta-hCG concentrations on days 11, 14 and 21 after OR were significantly lower in the group of patients with a miscarriage compared to the group with a term delivery (p = 0.02, < 0.001 and < 0.001 respectively). With the cut-off values of 15 mIU/mL, 80 mIU/mL and 1500 mIU/mL on days 11, 14 and 21, the positive predictive value was 89%, 93% and 92%. The negative predictive value was 28%, 40% and 54%.


CONCLUSIONS
The predictive value of a single beta-hCG measurement for pregnancy outcome after single blastocyst transfer increases between days 11 and 21 after OR. A single beta-hCG measurement on day 14 may balance the accuracy of prediction and the necessity of an early reassuring test.
Ochsenkühn
Robert
R
Department of Obstetrics and Gynecology - Grosshadern University Hospital, Ludwig-Maximilians-University, Marchioninistrasse 15, 81377 Munich, Germany. Robert.Ochsenkuehn@med.uni-muenchen.de
Arzberger
Andrea
A
von Schönfeldt
Viktoria
V
Engel
Jutta
J
Thaler
Christian J
CJ
Noss
Ulrich
U
D000328:Adult; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood*; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D054315:Oocyte Retrieval; D011237:Predictive Value of Tests; D011247:Pregnancy; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors
D018997:Chorionic Gonadotropin, beta Subunit, Human