Involvement of Na+/Ca2+ exchanger in pentylenetetrazol-induced convulsion by use of Na+/Ca2+ exchanger knockout mice.
Involvement of Na+/Ca2+ exchanger (NCX) in pentylenetetrazol (PTZ)-induced convulsion by use of NCX knockout mice and the selective ligand SEA0400 to NCX was examined. In the SEA0400-administered group, the latency to clonic convulsion was extended into 210 s, although the latency to clonic convulsion was observed until 100 s in control group. SEA0400 had little effect on bicuculline-induced clonic seizure nicotine-induced wild running and 4-aminopyridine-induced tonic flexion, respectively. Tonic flexion convulsion was occurred three fifth in the wild type mice group by administration of PTZ, but tonic flexion was not observed in NCX1 knockout mice groups. These results suggest that NCX is involved in inhibitory action in PTZ-induced convulsion.
Saito
Ryo
R
Department of Physiology and Pharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1Nanakuma, Sawara-ku, Fukuoka 814-0180, Japan. rsaito@fukuoka-u.ac.jp
Kaneko
Erina
E
Tanaka
Yusuke
Y
Honda
Kenji
K
Matsuda
Toshio
T
Baba
Akemichi
A
Komuro
Issei
I
Kita
Satomi
S
Iwamoto
Takahiro
T
Takano
Yukio
Y
D000818:Animals; D003292:Convulsants / Q000633:toxicity*; D051379:Mice; D018345:Mice, Knockout; D010433:Pentylenetetrazole / Q000633:toxicity*; D012640:Seizures / Q000139:chemically induced* / Q000503:physiopathology; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000502:physiology*
D003292:Convulsants; D019831:Sodium-Calcium Exchanger; D010433:Pentylenetetrazole