title: THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT.
abstract: 1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component. 2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 10(-5) in phosphate buffer of ionic strength 0.2 at pH 7.7, and with a sedimentation constant of 6.4 x 10(-13) in potassium chloride of ionic strength 0.2. 3. End-piece and 4th component were found together in a euglobulin fraction of serum which contained 10.3 per cent carbohydrate and had an electrophoretic mobility of 4.2 x 10(-5) in phosphate buffer of ionic strength 0.2 at pH 7.7.
authors.affiliation: Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland.
authors.forename: L
authors.initials: L
authors.lastname: Pillemer
authors.forename: E E
authors.initials: EE
authors.lastname: Ecker
authors.forename: J L
authors.initials: JL
authors.lastname: Oncley
authors.forename: E J
authors.initials: EJ
authors.lastname: Cohn