A novel and sensitive method for the detection of deoxynivalenol in food by time-resolved fluoroimmunoassay.
In order to detect deoxynivalenol (DON) contamination in cereals, a time-resolved fluoroimmunoassay (TRFIA) was developed. By use of DON-bovine serum albumin (DON-BSA), anti-DON antibody, and europium-labeled goat anti-rabbit antibody, this study established an indirect competitive DON-TRFIA. The sensitivity of this method was high, with the detection limit of 0.01 ng/mL. The tests showed the sensitivity was 0.1 microg/kg in cereals and 0.1 ng/mL in beer. Its detection range was 0.01-100 ng/mL. Within the detection range, the intra- and inter-batch CVs of the DON-TRFIA were 3.6% and 8.2%. Data obtained from corn and beer samples by TRFIA and ELISA were in good agreement. This assay did not cross-react with zearalenone (ZEN) and nivalenol (NIV) which commonly exists in corn. This study suggests that DON-TRFIA is a simple, fast and economic method for screening large quantities of samples, and has good prospects of application.
Wang
Ke
K
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu.
Huang
Biao
B
Zhang
Jue
J
Zhou
Bin
B
Gao
Lei
L
Zhu
Lan
L
Jin
Jian
J
D002523:Edible Grain / Q000737:chemistry*; D015200:Fluoroimmunoassay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
D014255:Trichothecenes; C007262:deoxynivalenol