Thinner inhalation effects on oxidative stress and DNA repair in a rat model of abuse.
Humans can come into contact with thinner by occupational exposure or by intentional inhalation abuse. Numerous studies of workers for genotoxic effects of thinner exposure have yielded conflicting results, perhaps because co-exposure to variable other compounds cannot be avoided in workplace exposure studies. In contrast, there is no data concerning the genotoxic effects of intentional inhalation abuse. The aim of this project was to examine the genotoxic effects of thinner inhalation in an animal model of thinner abuse (rats exposed to 3000 ppm toluene, a high solvent concentration over a very short, 15 min time period, twice a day for 6 weeks). The data presented here provides evidence that thinner inhalation in our experimental conditions is able to induce weight loss, lung abnormalities and oxidative stress. This oxidative stress induces oxidative DNA damage that is not a characteristic feature of genotoxic damage. No significant difference in DNA damage and DNA repair (biomarkers of genotoxicity) in lymphocytes from thinner-treated and control rats was found. Lead treatment was used as a positive control in these assays. Finally, bone marrow was evaluated as a biomarker of cellular alteration associated with thinner inhalation. The observed absence of hemopoietic and genetic toxicity could be explained in part by the absence of benzene, the only carcinogenic component of thinner; however, benzene is no longer a common component of thinner. In conclusion, thinner did not cause genotoxic effects in an experimental model of intentional abuse despite the fact that thinner inhalation induces oxidative stress.
Martínez-Alfaro
Minerva
M
Departamento de Neurobiología del Desarrollo y Neurofisología, Instituto de Neurobiología, UNAM Campus, Querétaro, México. alfarominerva@yahoo.fr
Cárabez-Trejo
Alfonso
A
Gallegos-Corona
Marco-Antonio
MA
Pedraza-Aboytes
Gustavo
G
Hernández-Chan
Nancy Georgina
NG
Leo-Amador
Guillermo Enrique
GE
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004195:Disease Models, Animal; D019570:Inhalation Exposure / Q000009:adverse effects*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008171:Lung Diseases / Q000097:blood / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D019966:Substance-Related Disorders / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D014050:Toluene / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity
D015415:Biomarkers; D009153:Mutagens; D012997:Solvents; D014050:Toluene; D008315:Malondialdehyde