title: Solid-phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid-based silica sorbent.
abstract: A new ionic liquid-based silica sorbent was developed by a process involving surface chemical modification of commercial silica using a synthesized ionic liquid. The obtained particles were successfully used as a special sorbent in a solid-phase extraction process to isolate liquiritin and glycyrrhizic acid from licorice. Different washing and elution solvents, such as water, methanol/water (v/v), and pure methanol were evaluated. Ionic liquid-based silica sorbent was compared with traditional C(18) sorbent and it exhibited higher selectivity. Quantitative analysis was carried out by using a C(18) column. Good linearities of two compounds were obtained from 5x10(-4) to 0.2 mg/mL (r(2)>0.99) with the relative standard deviations <1.0%. The target compounds in commercial herbal medicines containing licorice were determined, and the bound rates between the target compounds and protein were obtained by this sorbent.
authors.affiliation: Department of Chemical Engineering, Inha University, Incheon, Korea.
authors.forename: Minglei
authors.initials: M
authors.lastname: Tian
authors.forename: Wentao
authors.initials: W
authors.lastname: Bi
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Row
mesh_terms: D044950:Flavanones / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000302:isolation & purification*; D052578:Ionic Liquids; D012822:Silicon Dioxide; D052616:Solid Phase Extraction / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D044950:Flavanones; D005960:Glucosides; D052578:Ionic Liquids; D019695:Glycyrrhizic Acid; D012822:Silicon Dioxide; C512196:liquiritin