title: A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES OF THIS ENZYME.
abstract: A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.
authors.affiliation: Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, and the Biochemical Research Laboratory, Massachusetts General Hospital, Boston.
authors.forename: P C
authors.initials: PC
authors.lastname: Zamecnik
authors.forename: L E
authors.initials: LE
authors.lastname: Brewster
authors.forename: F
authors.initials: F
authors.lastname: Lipmann
mesh_terms: D001419:Bacteria; D001639:Bicarbonates; D005734:Gangrene; D005738:Gas Gangrene; D010740:Phospholipases
chemical_list: D001639:Bicarbonates; D010740:Phospholipases