Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme.
Pyranose dehydrogenase (PDH) is a flavin-dependant sugar oxidoreductase found in the family Agaricaceae, basidiomycetes that degrade lignocellulose-rich forest litter, and is catalytically related to the fungal enzymes pyranose 2-oxidase and cellobiose dehydrogenase. It has broad substrate specificity and displays similar activities with most sugar constituents of lignocellulose including disaccharides and oligosaccharides, a number of (substituted) quinones, and metal ions are suitable electron acceptors rather than molecular oxygen. In contrast to pyranose 2-oxidase and cellobiose dehydrogenase, which oxidize regioselectively at C-2 and C-1, respectively, PDH is capable of oxidation on C-1 to C-4 as well as double oxidations, depending on the nature of the substrate. This makes it a very interesting enzyme for biocatalytic applications, as many of the reaction products are otherwise unaccessible by chemical or enzymatic means. PDH was characterized in detail in a limited number of fungi, and the first encoding genes were isolated only recently. We report here, for the first time, the heterologous expression of one of these genes, encoding the major PDH protein in Agaricus meleagris, in the filamentous fungi Aspergillus nidulans, and Aspergillus niger.
Pisanelli
Ines
I
Department of Food Sciences and Technology, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria.
Kujawa
Magdalena
M
Gschnitzer
Doris
D
Spadiut
Oliver
O
Seiboth
Bernhard
B
Peterbauer
Clemens
C
D000364:Agaricus / Q000201:enzymology*; D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000235:genetics; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D011714:Pyrans / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D005656:Fungal Proteins; D011714:Pyrans; D011994:Recombinant Proteins; D002237:Carbohydrate Dehydrogenases