Protective effect of resveratrol against lipopolysaccharide-induced oxidative stress in rat brain.
OBJECTIVE
To study the protective effect of resveratrol on endotoxemia-induced neurotoxicity.


METHODS
Rats were pre-treated during 7 days with 20 mg kg(-1) body weight (b.w.) resveratrol and challenged with a single dose of lipopolysaccharide (LPS: 8 mg kg(-1) b.w.) for 24 hours. Brains were harvested to determine LPS-induced lipoperoxidation level, antioxidant enzyme activities, nitric monoxide (NO) and iron distribution as well as the impact of resveratrol on these parameters.


RESULTS
Resveratrol counteracted LPS-induced brain malondialdehyde (MDA) level and antioxidant enzyme activities depletion as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). Resveratrol also reversed LPS-induced brain and plasma NO elevation as well as iron sequestration from plasma to brain compartment.


CONCLUSIONS
The data suggest that resveratrol is capable of alleviating LPS-induced neurotoxicity by a mechanism that may involve iron shuttling proteins.
Sebai
Hichem
H
Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, Zarzouna, Tunisie.
Gadacha
Wafa
W
Sani
Mamane
M
Aouani
Ezzedine
E
Ghanem-Boughanmi
Néziha
N
Ben-Attia
Mossadok
M
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019446:Endotoxemia / Q000150:complications; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008070:Lipopolysaccharides; D013267:Stilbenes; D009569:Nitric Oxide; D008315:Malondialdehyde; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D000077185:Resveratrol