Effects of resin or charcoal treatment on fetal bovine serum and bovine calf serum.
BACKGROUND
Charcoal- or resin-stripping of fetal bovine serum (FBS) or bovine calf serum (BCS) intended for supplementation of cell culture media is widely practiced to remove a variety of endogenous compounds, including steroid, peptide, and thyroid hormones. The possibility that stripping removes other biologically relevant factors from serum may not be appreciated.


METHODS
In this report, standardized clinical laboratory testing methods were used to assess the effects of resin- and charcoal-stripping on content in FBS and BCS of more than 25 analytes in the sera.


CONCLUSIONS
In addition to hormones, the serum constituents affected by stripping are certain vitamins, electrolytes, enzyme activities, and metabolites.
Cao
Zhimin
Z
Laboratory of Molecular Diagnostics, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA.
West
Clint
C
Norton-Wenzel
Carol S
CS
Rej
Robert
R
Davis
Faith B
FB
Davis
Paul J
PJ
Rej
Robert
R
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards; D002606:Charcoal / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry / Q000592:standards; D004573:Electrolytes / Q000032:analysis / Q000302:isolation & purification; D020536:Enzyme Activators / Q000032:analysis / Q000302:isolation & purification; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D006728:Hormones / Q000032:analysis / Q000302:isolation & purification; D012117:Resins, Synthetic / Q000494:pharmacology*; D044967:Serum / Q000737:chemistry / Q000187:drug effects*; D014815:Vitamins / Q000032:analysis / Q000302:isolation & purification
D003470:Culture Media; D004573:Electrolytes; D020536:Enzyme Activators; D004798:Enzymes; D006728:Hormones; D012117:Resins, Synthetic; D014815:Vitamins; D002606:Charcoal