Nanoscale in situ morphological study of proteins immobilized on gold thin films.
The nanoscale organization of acetylcholinesterase (AChE) and of its polyclonal antibody immobilized on gold thin films was studied by means of Energy Dispersive X-ray Reflectometry (EDXR) and Atomic Force Microscopy (AFM). The macromolecules were alternatively deposited over a self-assembled monolayer (SAM) of N-hydroxysuccinimide esters of thioctic acid. The measurements, collected in situ at subsequent deposition stages of the device, gave information on the distribution of the macromolecules on the surface showing that both the proteins can bind covalently to the SAM. In addition to this, we demonstrated that the antigen-antibody reaction takes place when one of the two reactants is anchored to the surface.
Scaramuzzo
Francesca A
FA
Dipartimento di Chimica, Sapienza Università di Roma, Piazzale A.Moro, 5 - Roma 00185, Italy.
Salvati
Romina
R
Paci
Barbara
B
Generosi
Amanda
A
Rossi-Albertini
Valerio
V
Latini
Alessandro
A
Barteri
Mario
M
D000110:Acetylcholinesterase / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
D055910:Antibodies, Immobilized; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D006046:Gold; D000110:Acetylcholinesterase