title: Some properties of beta-1,2-mannosyltransferases related to the biosynthesis of the acid-labile oligomannosyl side chains in Candida albicans NIH B-792 strain cells.
abstract: We detected the beta-1,2-mannosyltransferases (beta-1,2-MTs), which participate in the biosynthesis of oligomannosyl side chains in the mannan acid-labile fraction, in a particulate insoluble fractions prepared from Candida albicans NIH B-792 strain cells grown at 27 degrees C and at 37 degrees C in a yeast extract-added Sabouraud liquid medium (YSLM). The beta-1,2-MT VI-6 prepared from the cells grown at 27 degrees C exhibited the maximum activity at pH 7.0 and at 30 degrees C. The beta-1,2-MT VI-6 activity was only slightly affected by Mn2+, Mg2+, Ca2+, and ethylenediaminetetraacetic acid, but completely inhibited by Zn2+ and Ni2+. The beta-1,2-MT activities from the cells grown at 37 degrees C were lower than that from the cells grown at 27 degrees C, especially on the longer beta-1,2-mannooligosaccharides than tetraose.
authors.affiliation: Department of Infection and Host Defense, Tohoku Pharmaceutical University, Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Goto
authors.forename: Akifumi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shibata
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okawa
mesh_terms: D002176:Candida albicans / Q000201:enzymology / Q000378:metabolism*; D008358:Mannose / Q000096:biosynthesis* / Q000737:chemistry; D008364:Mannosyltransferases / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D008364:Mannosyltransferases; D008358:Mannose