[Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides].
OBJECTIVE
To analyze and compare the cell growth and accumulation of flavonoids and chlorogenic acid in the callus and suspension cell of Eucommia ulmoides.


METHODS
The callus induced from the leaf of E. ulmoides seedlings were suspended in liquid medium. The time courses of cell growth and yields of flavonoids and chlorogenic acid were studied.


RESULTS
The highest contents of flavonoids and chlorogenic acid in the callus were 13.46, 1.712 mg x g(-1), respectively, while the contents of these two secondary metabolites were 16.63, 3.93 mg x g(-1) in suspension cell culture correspondingly.


CONCLUSIONS
Comparing with callus, the suspension cell showed a short growth period and high growth rate with a remarkable high content of flavonoids and chlorogenic acid.
Zhang
Zhibin
Z
Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, College of Life Sciences Jiangxi Normal University, Nanchang 330022, China.
Yan
Riming
R
Qiu
Xiaofang
X
Zeng
Qinggui
Q
You
Hai
H
Zhu
Du
D
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000032:analysis / Q000378:metabolism*; D031283:Eucommiaceae / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism*
D005419:Flavonoids; D002726:Chlorogenic Acid