title: The opposite correlation between calcium ion and cyclic-AMP regarding the activation of microsomal triglyceride transfer protein in rat liver.
abstract: In this study, the effects of Ca(2+) and cyclic adenosine monophosphate (cAMP) on microsomal triglyceride (TG) transfer protein (MTP) activity were investigated in rat liver. MTP activity was high when liver contained low levels of cAMP, which was induced by administration of glucose, or high levels of total Ca(2+) and TG. However, MTP activity increased by high levels of Ca(2+) and TG was reduced in a dose-dependent manner by treatment with dibutyryl-cAMP (db-cAMP), a cAMP analogue. Conversely, when homogenates of liver from normal rats, with low levels of total Ca(2+) and high levels of cAMP, were incubated with thapsigargin, a Ca(2+)-inducer, MTP activity was increased in a dose-dependent manner compared to control. Therefore, our results suggest that high levels of Ca(2+) cause hypertriglyceridemia through the elevation of MTP activity, as opposed to high levels of cAMP, which suppress MTP activity and inhibit hypertriglyceridemia.
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, Daejeon 302-718, Korea.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Hyeong-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Young-Bin
authors.initials: YB
authors.lastname: Yu
authors.forename: Hyo-Chan
authors.initials: HC
authors.lastname: Kang
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Lee
authors.forename: Man-Hee
authors.initials: MH
authors.lastname: Rhee
authors.forename: Jae-Youl
authors.initials: JY
authors.lastname: Cho
authors.forename: Hwa-Jin
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D019284:Thapsigargin / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein; D003994:Bucladesine; D019284:Thapsigargin; D000242:Cyclic AMP; D005947:Glucose; D002118:Calcium