[Identification on Syngnathus and its adulterants with random amplified polymorphic DNA].
OBJECTIVE
To study the identification method of Syngnathus and its adulterants.


METHODS
Random amplified polymorphic DNA (RAPD) was used to construct a dendrogram by UPGMA method based on Nei & Li's coefficient and a genetic affinity pattern for Syngnathus acus, Solenognathus hardwickii, Syngnathoides biaculeatus, Trachyrhamphus serratus, Halicampus koilomatodon, Microphis boaja.


RESULTS
Four primers, LJ04, LJ09, LJ16 and LJ19, from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification. The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants.


CONCLUSIONS
RAPD is suitable to identify Syngnathus and its adulterants.
Wu
Yan
Y
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Liu
Jia
J
Wang
Mengyue
M
Ju
Peijun
P
Li
Xiaobo
X
D000818:Animals; D002681:China; D017931:DNA Primers / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D023701:Smegmamorpha / Q000145:classification / Q000235:genetics*
D017931:DNA Primers