title: Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections.
abstract: Since traditional diagnosis of dermatophyte infections is slow, we present a rapid new PCR test for detection of Trichophyton spp., Microsporum canis and M. audouinii infections. The performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human DNA control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected with M. canis; and two samples from un-infected control animals. DNA was prepared by a 10-min procedure from pure cultures as previously described. The 302 bp PCR product was obtained for 35/35 Trichophyton isolates (10 species included) and the 279 bp for 3/3 M. canis and 4/4 M. audouinii samples. None of the 2 E. floccosum, 11 M. gypseum, 3 M M. persicolor or 12 control samples (yeast, mould, human DNA) were positive with either of the two PCR tests. Among the patient specimens, seven were T. rubrum positive, two for T. mentagrophytes, one was positive for T. tonsurans and 15 were dermatophyte negative by routine investigation (culture and/or pan-dermatophyte + T. rubrum multiplex PCR). The PCR results with our procedures were in 100% agreement with these results. Finally, the Microsporum PCR was positive for 10/10 guinea pig specimens from infected animals but for 0/2 of the control animal samples. The evaluation of the two PCR tests indicated excellent sensitivity and specificity.
authors.affiliation: Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark. abrill@chem.pg.gda.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Brillowska-Dabrowska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Swierkowska
authors.forename: Ditte Marie
authors.initials: DM
authors.lastname: Lindhardt Saunte
authors.forename: Maiken Cavling
authors.initials: MC
authors.lastname: Arendrup
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology*; D006168:Guinea Pigs; D006801:Humans; D008864:Microsporum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014249:Trichophyton / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal