title: NYGGF4 homologous gene expression in 3T3-L1 adipocytes: regulation by FFA and adipokines.
abstract: NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects and is involved in insulin resistance. In the present study, the mRNA expression of NYGGF4 homologous genes was examined in the 3T3-L1 cell line. The NYGGF4 mRNAs were expressed at low levels in the 3T3-L1 preadipocytes. During the conversion of 3T3-L1 preadipocytes to adipocytes, the expression of NYGGF4 mRNA was upregulated. On the 8th day after induction of differentiation, the NYGGF4 mRNA levels peaked and remained high. Free fatty acids (FFA) and tumor necrosis factor-α (TNFα) could upregulate NYGGF4 mRNA expression in 3T3-L1 adipocytes, while interleukin-6 (IL-6), leptin, and resistin exerted an inhibitory effect. The results suggest that the expression of NYGGF4 mRNA is affected by a variety of factors that are related to insulin sensitivity. It is likely that NYGGF4 may be an important mediator in the development of obesity-related insulin resistance.
authors.affiliation: Department of Endocrinology, The 82th Hospital of the People's Liberation Army, 223001, Huaian, China.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Jia-lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Chen-bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Xia
authors.initials: X
authors.lastname: Chi
authors.forename: Qin
authors.initials: Q
authors.lastname: Hong
authors.forename: Yu-zhu
authors.initials: YZ
authors.lastname: Peng
authors.forename: Xi-rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D054392:Adipokines / Q000494:pharmacology*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology; D020738:Leptin / Q000494:pharmacology; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D052243:Resistin / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D054392:Adipokines; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha