A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES OF THIS ENZYME.
A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.
Zamecnik
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Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, and the Biochemical Research Laboratory, Massachusetts General Hospital, Boston.
Brewster
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Lipmann
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D001419:Bacteria; D001639:Bicarbonates; D005734:Gangrene; D005738:Gas Gangrene; D010740:Phospholipases
D001639:Bicarbonates; D010740:Phospholipases