title: Genome diversity in the genera Fructobacillus, Leuconostoc and Weissella determined by physical and genetic mapping.
abstract: Pulsed-field gel electrophoresis analysis of chromosomal single and double restriction profiles of 17 strains belonging to three genera of 'Leuconostocaceae' was done, resulting in physical and genetic maps for three Fructobacillus, six Leuconostoc and four Weissella strains. AscI, I-CeuI, NotI and SfiI restriction enzymes were used together with Southern hybridization of selected probes to provide an assessment of genomic organization in different species. Estimated genome sizes varied from 1408 kb to 1547 kb in Fructobacillus, from 1644 kb to 2133 kb in Leuconostoc and from 1371 kb to 2197 kb in Weissella. Other genomic characteristics of interest were analysed, such as oriC and terC localization and rrn operon organization. The latter seems markedly different in Weissella, in both number and disposition in the chromosome. Comparisons of intra- and intergeneric features are discussed in the light of chromosome rearrangements and genomic evolution.
authors.affiliation: Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências da Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Ivo M
authors.initials: IM
authors.lastname: Chelo
authors.affiliation: Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências da Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Líbia
authors.initials: L
authors.lastname: Zé-Zé
authors.affiliation: Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências da Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Rogério
authors.initials: R
authors.lastname: Tenreiro
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D016680:Genome, Bacterial; D006094:Gram-Positive Bacteria / Q000235:genetics*; D007934:Leuconostoc / Q000235:genetics*; D056584:Leuconostocaceae / Q000235:genetics*; D020161:Physical Chromosome Mapping; D013045:Species Specificity
chemical_list: D004262:DNA Restriction Enzymes