title: [Effects of total flavonoids from Rhizoma Drynariae medicated serum on proliferation, differentiation, cell cycle and apoptosis of osteoblasts in vitro].
abstract: OBJECTIVE
  To observe the effects of total flavonoids from Rhizoma Drynariae medicated serum on cell proliferation, differentiation, cell cycle and apoptosis of rats' osteoblasts cultured in vitro.
  
  
  METHODS
  The osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP, PI and Annexin V/PI methods were used to observe the proliferation, activity of alkaline phosphatase (ALP), every stage cells in the cell cycle, and the ratio of apoptosis cells by different concentrations of total flavonoids from Rhizoma Drynariae medicated serum at different time points.
  
  
  RESULTS
  Different concentrations of total flavonoids from Rhizoma Drynariae medicated serum could significantly enhance the cell proliferation rate and ALP activity (P < 0.05), more cells in S stage and less cells apoptosised than that of the untreated osteoblasts (P < 0.05).
  
  
  CONCLUSIONS
  Total flavonoids from Rhizoma Drynariae medicated serum could promote the proliferation, differentiation, increase S stage cells, and reduce the ratio of apoptosis cells, which reveals that they have anti-osteoporosis activity.
authors.affiliation: Department of Orthopedics, Second Hospital Affilicated to Medical College of Xi'an Jiaotong University, Xi'an 710004, China. zjxjtu2001@stu.xtu.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Hao-Peng
authors.initials: HP
authors.lastname: Li
authors.forename: Ping-Lin
authors.initials: PL
authors.lastname: Yang
authors.forename: Yi-Heng
authors.initials: YH
authors.lastname: Liu
authors.forename: Bao-Hui
authors.initials: BH
authors.lastname: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010024:Osteoporosis / Q000517:prevention & control; D029621:Polypodiaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005419:Flavonoids; D000469:Alkaline Phosphatase