title: Isolation and characterization of a novel short-chain alcohol dehydrogenase gene from Panax ginseng.
abstract: The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses.
authors.affiliation: Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Suwon 449-701, Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Ju-Sun
authors.initials: JS
authors.lastname: Shim
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Jung
authors.forename: Hwa
authors.initials: H
authors.lastname: Sun
authors.forename: Jun-Gyo
authors.initials: JG
authors.lastname: In
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D015139:Blotting, Southern; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D005894:Panax / Q000201:enzymology* / Q000235:genetics*; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D018744:DNA, Plant; D012333:RNA, Messenger; D000426:Alcohol Dehydrogenase