A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice.
Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.
Yamaji
Naoki
N
Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan.
Huang
Chao Feng
CF
Nagao
Sakiko
S
Yano
Masahiro
M
Sato
Yutaka
Y
Nagamura
Yoshiaki
Y
Ma
Jian Feng
JF
D000535:Aluminum / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology*
D010940:Plant Proteins; D014157:Transcription Factors; D000535:Aluminum