title: Expression of Ca-binding protein recoverin in normal, surviving, and regenerating retina of Pleurodeles waltl adult triton.
abstract: Immunohistochemical study of the expression of recoverin (photoreceptor protein) in the retina of Pleurodeles waltl adult triton was carried out in health, during regeneration after removal, and under conditions of long-lasting detachment. Studies with polyclonal (monospecific) antibodies to recoverin showed that normally it is present in the internal segment, connective cilium, in distal portions of the external segments of cones and rods, and in Landolt clubs of displaced bipolar cells. Detachment of the retina is associated with translocation of recoverin from the photoreceptor processes to perikaryons, and the content of recoverin-positive displaced bipolar cells increases. During regeneration of the retina after its excision via conversion of the pigmented epithelial cells, recoverin is synthesized in the prospective photoreceptor perikaryons and then accumulates in the forming inner segments. Hence, recoverin can serve as a reliable marker in studies of photoreceptor differentiation and functioning during regeneration or survival of the retina.
authors.affiliation: N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. leonore@mail.ru
authors.forename: E N
authors.initials: EN
authors.lastname: Grigoryan
authors.forename: A V
authors.initials: AV
authors.lastname: Bazhin
authors.forename: M S
authors.initials: MS
authors.lastname: Krasnov
authors.forename: P P
authors.initials: PP
authors.lastname: Philippov
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D010999:Pleurodeles / Q000378:metabolism*; D051603:Recoverin / Q000378:metabolism*; D012038:Regeneration; D012160:Retina / Q000378:metabolism* / Q000502:physiology
chemical_list: D051603:Recoverin