Quantitative determination of cerebrospinal fluid bilirubin on a high throughput chemistry analyzer.
BACKGROUND
Subarachnoid hemorrhage is a condition with high rates of mortality and morbidity. The diagnosis requires an urgent cerebral computed tomography scan and also a lumbar puncture if the scan fails to demonstrate intracranial blood. In Sweden the cerebrospinal fluid (CSF) is analyzed by spectrophotometric scanning for the presence of hemoglobin and bilirubin. The aim of the study was to develop a quantitative diazo reagent based analysis of cerebrospinal fluid bilirubin as a replacement for spectrophotometric scanning.


METHODS
The CSF bilirubin assay on an Architect C8000 chemistry analyzer was compared with spectrophotometry using patient samples.


RESULTS
The method correlates with spectrophotometry, has a good linearity and precision.


CONCLUSIONS
Quantitative bilirubin measurement offers shorter turnaround times, simplifies the interpretation of the results and reduces work load in comparison with spectrophotometry.
Ahmed
Degmo Said
DS
Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden.
Larsson
Anders
A
Hillered
Lars
L
Wande
Birgitta
B
Ryden
Ingvar
I
D001663:Bilirubin / Q000134:cerebrospinal fluid*; D004355:Drug Stability; D006801:Humans; D015994:Incidence; D008027:Light; D013053:Spectrophotometry; D013129:Spinal Puncture / Q000379:methods; D020521:Stroke / Q000209:etiology; D013345:Subarachnoid Hemorrhage / Q000000981:diagnostic imaging* / Q000453:epidemiology / Q000401:mortality; D013548:Sweden / Q000453:epidemiology; D014057:Tomography, X-Ray Computed
D001663:Bilirubin