title: [Comparison of content of clemastanin B of Radix Isatidis in different growing areas].
abstract: OBJECTIVE
  To develop an HPLC method for determination of clemastanin B which has anti-viral activity in Radix Isatidis and compare the contents of clemastanin B in the drugs from different origins.
  
  
  METHODS
  The samples were separated on an ZORBAX SB-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (11:89). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 225 nm. Column temperature was 30 degrees C.
  
  
  RESULTS
  The linear range of clemastanin B was 0.0615-1.8441 microg (r = 0.9995), the average recovery was 97.74%, RSD was 1.4% (n=9). The contents of clemastanin B were in the range of 0.269-0.900 mg x g(-1) in Radix Isatidis from different origins.
  
  
  CONCLUSIONS
  The method for quantitation of clemastanin B in Radix Isatidis was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Key Laboratory of Scientfic Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. anyhe2006@yahoo.com.cn
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: An
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Lili
authors.initials: L
authors.lastname: Chang
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
mesh_terms: D002681:China; D005663:Furans / Q000032:analysis*; D031219:Isatis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D005663:Furans; D010936:Plant Extracts; C507644:clemastanin B