title: THE STRUCTURE OF THE COLLODION MEMBRANE AND ITS ELECTRICAL BEHAVIOR : XII. THE PREPARATION AND PROPERTIES OF "MEGAPERMSELECTIVE" PROTAMINE COLLODION MEMBRANES COMBINING HIGH IONIC SELECTIVITY WITH HIGH PERMEABILITY.
abstract: The technique of Abrams and Sollner for the preparation of electropositive dried protamine collodion membranes has been improved. Porous collodion membranes cast on the outside of rotating tubes are treated for 48 hours with a solution of 2 per cent protamine sulfate buffered at pH 11. After being washed thoroughly the membranes are dried in air for several hours, soaked in water for several hours, and removed from the tubes. Further drying in air but without support shrinks the membranes slightly. The resulting membranes are designated "permselective" or "megapermselective" protamine collodion membranes. These membranes regularly give characteristic concentration potentials of -52 to -53 mv. and (in 0.1 M KCl) resistance of 0.5 to 15 ohms per membrane of 50 cm.(2) area. This resistance is several orders of magnitude smaller than that of the conventional dyestuff- and alkaloid-impregnated positive membranes. The megapermselective protamine collodion membranes can be kept either dry or in water for prolonged periods without detectable deterioration. They are quite smooth, have a regular shape, and stand considerable handling without breakage. The megapermselective protamine collodion membranes are the electropositive analogues of the electronegative megapermselective collodion membranes described by Carr and Sollner.
authors.affiliation: Department of Physiology, University of Minnesota, Minneapolis.
authors.forename: C W
authors.initials: CW
authors.lastname: Carr
authors.forename: H P
authors.initials: HP
authors.lastname: Gregor
authors.forename: K
authors.initials: K
authors.lastname: Sollner