[Effects of androgenic anabolic steroids on the uterus and reproductive parameters of adult female rats].
OBJECTIVE
to evaluate the effects of the administration of two synthetic steroids in the uterus morphology and in the reproductive parameters of adult female rats.


METHODS
divided into four experimental groups: control (C; physiological solution); treated with nandrolone decanoate (DN; 7.5 mg/kg of body weight); with a testosterone esters compound (T; 7.5 mg/kg); and simultaneously with DN and T (7.5 mg/kg of each steroid), in a single intraperitoneal weekly dose, for eight weeks. Five females of each group were sacrificed and the uterine horns were collected, weighted and prepared for histological and morphometrical evaluation. The remaining rats were mated with normal male rats for reproductive parameters evaluation, composing the groups treated during the pre-gestational period. Another group of 20 female rats were treated during the gestational period (7th-14th days). For data analysis, the Kruskal-Wallis non-parametric variance analysis was used, followed by the test of Dunn or of Student-Newman-Keus (5% significance level).


RESULTS
there was a significant body weight increase in the androgenized females (ND: 305+/-50; T: 280+/-35; ND+T: 275+/-30 versus C: 255+/-22 g; p<0.05). Uterine weight was not affected by the steroidal treatment (ND: 0.6+/-0.2; T: 0.4+/-0.04; ND+T: 0.7+/-0.1 versus C: 0.4+/-0.09 g). All the androgenized females presented estral acyclicity and endometrium characterized by papilliferous luminal lining, oedematous stroma with hemorrhagic areas and secretory activity. There were changes in the morphometrical thickness parameters of the luminal epithelium, myometrium and perimetrium in the androgenized groups. None of the female rats got pregnant when treated with steroids in the pre-gestational period and the treatment during organogenesis affected negatively the reproductive parameters.


CONCLUSIONS
steroidal agents alter the uterine structure and impair fertility and gestational outcome in female rats.
Camargo
Isabel Cristina Cherici
IC
Departamento de Ciências Biológicas, Universidade Estadual Paulista Júlio de Mesquita Filho, Assis, SP, Brasil.
Gaspar
Ana Luisa Camolezi
AL
Frei
Fernando
F
Mesquita
Suzana de Fátima Paccola
Sde F
D000367:Age Factors; D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D005260:Female; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects* / Q000473:pathology
D045930:Anabolic Agents; D013739:Testosterone; D009277:Nandrolone; D000077603:Nandrolone Decanoate