title: Low level of O2 inhibits commitment of cultured mesenchymal stromal precursor cells from the adipose tissue in response to osteogenic stimuli.
abstract: Mesenchymal stromal precursor cells from human lipoaspirate (lMSC) cultured at 5% O(2) formed 50% less mineralized matrix in response to osteogenic induction than cells cultured under standard conditions (20% O(2)). After lMSC percultured at 5% O(2) were transferred to normoxic conditions (20% O(2)), they produced the same amount of matrix as lMSC permanently cultured at 20% O(2). Hence, hypoxia inhibited the commitment of lMSC under the effect of osteogenic stimuli, which can be important in reparative and regenerative medicine.
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow. grinakovskaya@imbp.ru
authors.forename: O S
authors.initials: OS
authors.lastname: Grinakovskaya
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Rylova
authors.forename: G Yu
authors.initials: GY
authors.lastname: Kosovsky
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D010100:Oxygen