[Properties of hybridization isotherms upon binding of ligands on microchips].
A simple thermodynamic model describing the microarray oligo-target hybridization has been constructed. The relationship between the hybridization signal intensity and Gibbs free energy for oligo-target duplex formation has been considered. The behavior of this function, which we called energetical hybridization isotherm, in response to target concentration change was modeled at different ratios of oligo-probes/target concentrations. The results of modeling were compared with the relevant and currently available data from microarray adsorption experiments.
Golovkin
M V
MV
Matveeva
O V
OV
Nechipurenko
Iu D
IuD
D015342:DNA Probes / Q000737:chemistry*; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D013816:Thermodynamics
D015342:DNA Probes