Construction of smart glutathione peroxidase mimic based on hydrophilic block copolymer with temperature responsive activity.
A temperature-sensitive block copolymer (PAAm-b-PNIPAAm-Te) with a glutathione peroxidase-like active site was synthesized via ATRP. As a new glutathione peroxidase (GPx) mimic, it displays typical saturation kinetic behaviors and high catalytic activity. More importantly, the catalytic activity of the polymer can be well modulated by changing the temperature. The experiments proved that a change in the self-assembly structure of the polymer plays a key role for the modulation of catalytic activity. As a contrast, another block copolymer, PAAm-Te-b-PNIPAAm, was synthesized. In comparison with PAAm-b-PNIPAAm-Te, the different temperature dependent catalytic behavior seen further indicates that the micellar structure plays an important role in modulating the catalytic activity of the smart enzyme model.
Huang
Xin
X
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Yin
Yanzhen
Y
Jiang
Xu
X
Tang
Yong
Y
Xu
Jiayun
J
Liu
Junqiu
J
Shen
Jiacong
J
D055162:Biocatalysis; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D018716:Molecular Mimicry; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
D011108:Polymers; D005979:Glutathione Peroxidase