title: CPm gene diversity in field isolates of Citrus tristeza virus from Colombia.
abstract: The nucleotide sequence diversity of the CPm gene from 28 field isolates of Citrus tristeza virus (CTV) was assessed by SSCP and sequence analyses. These isolates showed two major shared haplotypes, which differed in distribution: A1 was the major haplotype in 23 isolates from different geographic regions, whereas R1 was found in isolates from a discrete region. Phylogenetic reconstruction clustered A1 within an independent group, while R1 was grouped with mild isolates T30 from Florida and T385 from Spain. Some isolates contained several minor haplotypes, which were very similar to, and associated with, the major haplotype.
authors.affiliation: Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, Colombia. oaoliverosg@unal.edu.co
authors.forename: Oscar Arturo
authors.initials: OA
authors.lastname: Oliveros-Garay
authors.forename: Natalhie
authors.initials: N
authors.lastname: Martinez-Salazar
authors.forename: Yanneth
authors.initials: Y
authors.lastname: Torres-Ruiz
authors.forename: Orlando
authors.initials: O
authors.lastname: Acosta
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000145:classification / Q000235:genetics*; D003105:Colombia; D014644:Genetic Variation; D006239:Haplotypes; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins