Chelex-based DNA isolation procedure for the identification of microbial communities of eggshell surfaces.
Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total communities of eggshell surfaces from six avian species.
Martín-Platero
Antonio Manuel
AM
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Peralta-Sánchez
Juan Manuel
JM
Soler
Juan José
JJ
Martínez-Bueno
Manuel
M
D000818:Animals; D000835:Animals, Wild; D001717:Birds / Q000196:embryology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000302:isolation & purification; D004528:Egg Shell / Q000382:microbiology*; D004625:Embryo, Nonmammalian / Q000196:embryology; D046109:Passeriformes / Q000382:microbiology; D011137:Polystyrenes; D011145:Polyvinyls; D020305:Strigiformes / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex