Murine fecal proteomics: a model system for the detection of potential biomarkers for colorectal cancer.
Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC-MS/MS to analyse fecal samples from Apc(Min/+) mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples.
Ang
Ching-Seng
CS
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, PO Box 2008, Royal Melbourne Hospital, Australia.
Rothacker
Julie
J
Patsiouras
Heather
H
Burgess
Antony W
AW
Nice
Edouard C
EC
D000367:Age Factors; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000737:chemistry*; D017491:Genes, APC; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins