title: Molecular cloning and expression analysis of ovary-specific transcript 1 (Pm-OSTI) of the giant tiger shrimp, Penaeus monodon.
abstract: Isolation and characterization of genes specifically expressed in ovaries are necessary for understanding sex differentiation and ovarian development processes in the giant tiger shrimp, Penaeus monodon. In this study, a transcript that significantly matched the polehole precursor was further characterized by RACE-PCR. The sequence obtained was 5151 bp in length and contained a coding region of 5031 bp corresponding to 1677 amino acids. This transcript was only expressed in ovaries but not in testes of Juveniles (N = 10) and broodstock (N = 22) of P. monodon. A tissue distribution analysis further confirmed ovary-specific expression of this transcript (called P. monodon ovary-specific transcript 1, Pm-OST1) in female broodstock. Expression levels of Pm-OST 1 in ovaries of juvenile P. monodon upon 5-HT Injection (33.9+/-6.40 g; 50 microg/g body weight) were significantly higher at 12-72 hours post Injection (P<0.05). Quantitative real-time PCR Indicated that Pm-OST1 was comparably expressed throughout ovarian development in normal P. monodon broodstock (P>0.05). However, the expression level of Pm-OST1 was significantly higher in stage-III ovaries in eyestalk-ablated broodstock (P<0.05). Pm-OST1 was clearly localized in the ooplasm of previtellogenic and vitellogenic oocytes. Our results suggest that Pm-OST1 plays a functionally Important role in promoting the development of female germ cells and oocytes in P. monodon.
authors.affiliation: Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong Luang, Pathumthani 12120, Thailand. sirawut@biotec.or.th
authors.forename: Sirawut
authors.initials: S
authors.lastname: Klinbunga
authors.forename: Kanchana
authors.initials: K
authors.lastname: Sittikankaew
authors.forename: Vasin
authors.initials: V
authors.lastname: Yuvanatemiya
authors.forename: Rachanimuk
authors.initials: R
authors.lastname: Preechaphol
authors.forename: Sirikan
authors.initials: S
authors.lastname: Prasertlux
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamano
authors.forename: Piamsak
authors.initials: P
authors.lastname: Menasveta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D011506:Proteins; D012701:Serotonin