Important but little known (or forgotten) artifacts in protein biochemistry.
Many subtle and sometimes obscure artifacts exist that can have major effects on the outcomes of otherwise carefully performed experiments. This chapter focuses on a few of these artifacts involved in processes such as SDS gel sample preparation, buffers, Ni-chelate affinity and ion-exchange chromatography, urea preparation, use of plasticware, and filtration of protein samples.
Burgess
Richard R
RR
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin, USA.
D000042:Absorption / Q000502:physiology; D000818:Animals; D016477:Artifacts; D001671:Biochemistry / Q000379:methods* / Q000592:standards; D002021:Buffers; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010969:Plastics / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
D002021:Buffers; D010969:Plastics; D011506:Proteins