Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center.
A systematic and comprehensive phenotyping platform has been developed by the RIKEN ENU-mutagenesis project between 1999 and 2007. As a result of phenotype screening on this platform, we have discovered about 400 mutants as animal models for human diseases. All information regarding these mouse mutants is now available to the public through our home page (http://www.brc.riken.jp/lab/gsc/mouse/indexJ.html). In 2008, we reconstructed the existing phenotyping platform and built a new platform. The new system has a hierarchical structure, consisting of a fundamental pipeline that utilizes the existing platform and an additional pipeline, which is optimized for more in-depth phenotyping assays. Using this system, we have started to perform more comprehensive phenotyping of mouse mutants. We have opened this system to Japanese scientists as the Japanese Mouse Clinic. It is anticipated that existing mouse mutants will be reevaluated as disease models by identifying novel phenotypes on the new platform. We will share detailed information about the standard operating procedures (SOPs) of our phenotyping analyses with other related large-scale projects, such as the European Mouse Disease Clinic (EUMODIC) and the German Mouse Clinic (GMC). Moreover, we will contribute to international efforts to standardize mouse phenotype data by sharing annotation of mutant phenotypes, which are made by internationally standardized methods, with other related projects.
Wakana
Shigeharu
S
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center, Tsukuba City, Ibaraki, Japan.
Suzuki
Tomohiro
T
Furuse
Tamio
T
Kobayashi
Kimio
K
Miura
Ikuo
I
Kaneda
Hideki
H
Yamada
Ikuko
I
Motegi
Hiromi
H
Toki
Hideaki
H
Inoue
Maki
M
Minowa
Osamu
O
Noda
Tetsuo
T
Waki
Kazunori
K
Tanaka
Nobuhiko
N
Masuya
Hiroshi
H
Obata
Yuichi
Y
D000822:Animal Husbandry; D000818:Animals; D016208:Databases, Factual; D004195:Disease Models, Animal; D005260:Female; D016678:Genome; D006801:Humans; D007253:Information Centers / Q000458:organization & administration*; D007391:International Cooperation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains / Q000235:genetics*; D010641:Phenotype; D012015:Reference Standards