Rapid method for identification of six common species of mycobacteria based on multiplex SNP analysis.
A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobacterial species by the multiplex SNaPshot method. Of the 468 mycobacterial isolates, 464 (99.15%) could be correctly identified by this assay. The multiplex SNaPshot technique is a promising discriminatory tool for rapid and accurate identification of frequently encountered clinical mycobacterial species.
Wang
Hongxiu
H
Key Laboratory of Mycobacteria Tuberculosis, Shanghai Pulmonary Hospital affiliated to Tongji University, 507 Zhengming Rd., Shanghai 200433, People's Republic of China.
Yue
Jun
J
Han
Min
M
Yang
Jinghui
J
Zhao
Yanlin
Y
D001431:Bacteriological Techniques / Q000379:methods*; D005838:Genotype; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*