title: Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation.
abstract: To study the role of different DNA repair genes in the resistance of Deinococcus radiodurans to mono- and polychromatic UV radiation, wild-type strain and knockout mutants in RecA, PprA, and IrrE of D. radiodurans were irradiated with UV-C (254 nm), UV-(A + B) (280-400 nm) and UV-A (315-400 nm) radiation, and survival was monitored. The strain deficient in recA was highly sensitive to UV-C radiation compared to the wild-type, but showed no loss of resistance against irradiation with UV-(A + B) and UV-A, while pprA and irrE-deficient strains exhibited elevated sensitivity to UV-A and UV-(A + B) radiation. These results suggest that the repair of DNA double-strand breaks is essential after treatment with highly energetic UV-C radiation, whereas protection from oxidative stress may play a greater role in resistance to environmentally relevant UV radiation.
authors.affiliation: Institute of Aerospace Medicine, Radiation Biology Division, Research Group 'Astrobiology', German Aerospace Center (DLR), Linder Hoehe, 51147, Cologne, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Bauermeister
authors.forename: Esma
authors.initials: E
authors.lastname: Bentchikou
authors.forename: Ralf
authors.initials: R
authors.lastname: Moeller
authors.forename: Petra
authors.initials: P
authors.lastname: Rettberg
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D034301:Deinococcus / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D024881:Drug Resistance, Bacterial; D009154:Mutation; D018384:Oxidative Stress; D011938:Rec A Recombinases / Q000235:genetics / Q000502:physiology; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011938:Rec A Recombinases