Optimized DNA-targeting using triplex forming C5-alkynyl functionalized LNA.
Triplex forming oligonucleotides (TFOs) modified with C5-alkynyl functionalized LNA (locked nucleic acid) monomers display extraordinary thermal affinity toward double stranded DNA targets, excellent discrimination of Hoogsteen-mismatched targets, and high stability against 3?-exonucleases.
Sau
Sujay P
SP
Dept. of Chemistry, Univ. of Idaho, ID-83844, USA.
Kumar
Pawan
P
Anderson
Brooke A
BA
Østergaard
Michael E
ME
Deobald
Lee
L
Paszczynski
Andrzej
A
Sharma
Pawan K
PK
Hrdlicka
Patrick J
PJ
D000480:Alkynes; D001665:Binding Sites; D004247:DNA / Q000037:antagonists & inhibitors*; D016503:Drug Delivery Systems / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
D000480:Alkynes; D009841:Oligonucleotides; C477371:locked nucleic acid; C051802:triplex DNA; D004247:DNA