Monolithic silica capillary columns with immobilized cellulose tris(3,5-dimethylphenylcarbamate) for enantiomer separations in CEC.
Two types of monolithic silica capillary columns with an immobilized cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) selector were prepared for enantiomer separations in CEC. The monolithic columns were prepared by a sol-gel process in fused-silica capillaries. CDMPC was then either immobilized on a silica monolith through an intermolecular polycondensation of the cellulose derivative containing a triethoxysilyl group, or on a vinylized silica monolith through radical copolymerization of the cellulose derivative, which also contained a vinyl group. IR spectra confirmed the successful immobilization of CDMPC on both columns. Eleven chiral compounds were used to evaluate the enantioselectivity on both column types. Results indicated that the columns obtained via polycondensation had higher separation ability than those obtained via radical polymerization, and that they showed satisfactory run-to-run repeatability and stability. These new techniques thus provide strategies for preparing immobilized polysaccharide-based chiral silica monolithic capillary columns for chiral separations by means of CEC.
He
Chiyang
C
Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel-VUB, Brussels, Belgium.
Hendrickx
Ans
A
Mangelings
Debby
D
Smeyers-Verbeke
Johanna
J
Vander Heyden
Yvan
Y
D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004355:Drug Stability; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D048448:Phenylcarbamates / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; D011108:Polymers; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D012822:Silicon Dioxide; D002482:Cellulose