Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton.
To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation System) indicate that these genes are involved in 68 biochemical pathways. These findings suggest that the related defense response, gossypol biosynthesis pathway and other complex regulation may be associated with pigment gland morphogenesis in cotton. The results may provide a basis for further study and serve as a guide for related research.
Sun
Quan
Q
College of Bioinformation, Chongqing University of Posts and Telecommunications, 400065, Chongqing, China.
Cai
Yingfan
Y
Xie
Yongfang
Y
Mo
Jianchuan
J
Yuan
Youlu
Y
Shi
Yuzhen
Y
Li
Shengwei
S
Jiang
Huaizhong
H
Pan
Zheng
Z
Gao
Yunling
Y
Chen
Min
M
He
Xiaohong
X
D015536:Down-Regulation / Q000235:genetics; D017753:Ecosystem; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D003368:Gossypium / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
D012333:RNA, Messenger; D018749:RNA, Plant