title: Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis.
abstract: The wolf spider Lycosa singoriensis is a hunting spider with a widespread distribution in northwest China. The venom gland of spiders, which is a very specialized secretory tissue, can secrete abundant and complex toxin components. To extensively examine the transcripts expressed in the venom glands of L. singoriensis, we generated 833 expressed sequence tags (ESTs) from a directional cDNA library. Toxin-like sequences account for 69.1% of these ESTs, 17.3% are similar to cellular transcripts and 13.6% have no significant similarity to any known sequences. Here, we identified 223 novel toxin-like sequences, which can be classified into six different superfamilies; that means a novel potential source of ligands for varied ion channels was discovered. With the aid of Gene Ontology terms and homology to eukaryotic orthologous groups, the annotation of cellular transcripts revealed some cellular processes important for the toxin secretion of venom glands including protein synthesis, protein folding, tuned post-translational processing and trafficking, etc.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Yongqun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Chen
authors.forename: Xing
authors.initials: X
authors.lastname: Tang
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.forename: Xia
authors.initials: X
authors.lastname: Xiong
authors.forename: Meichi
authors.initials: M
authors.lastname: Wang
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Rong
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D002681:China; D005088:Exocrine Glands / Q000502:physiology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013111:Spider Venoms / Q000235:genetics*; D013112:Spiders / Q000235:genetics* / Q000502:physiology
chemical_list: D013111:Spider Venoms