title: Shrinking and development of lipid droplets in adipocytes during catecholamine-induced lipolysis.
abstract: Time-lapse observation of adipocytes during catecholamine-induced lipolysis clearly shows that shrinking of existing lipid droplets (LDs) occurs in some adipocytes and that small LDs are newly developed in almost all cells. Immunofluorescence imaging reveals that activation and localization of hormone-sensitive lipase (HSL) on the surface of LDs, which are required for conferring maximal lipolysis, are necessary for the shrinking of the LDs. However, not all adipocytes in which phosphorylated HSL is localized on LDs exhibit shrinking of LDs. The simultaneous shrinking and development of LDs yield apparent fragmentation and dispersion of LDs in adipocytes stimulated with catecholamine.
authors.affiliation: Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan. macci@eng.hokudai.ac.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nagayama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Toshio
authors.initials: T
authors.lastname: Taira
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Uchida
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Gohara
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D008066:Lipolysis; D051381:Rats; D002787:Sterol Esterase / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002395:Catecholamines; D014280:Triglycerides; D002787:Sterol Esterase