[Agrobacterium tumefaciens mediated Chitinase and beta-1,3-glucanase gene transformation for Pinellia ternata].
OBJECTIVE
To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.


METHODS
Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.


RESULTS
PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.


CONCLUSIONS
The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata
Jin
Bo
B
Life and science college of Zhejiang Chinese Medial University, Hangzhou 310053, China. jinbont@sina.com
Jiang
Fusheng
F
Yu
Meirong
M
Chen
Nipi
N
Ding
Zhishan
Z
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D030021:Pinellia / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology
D005656:Fungal Proteins; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase