The use of Macronet resins to recover gamma-decalactone produced by Rhodotorula aurantiaca from the culture broth.
During the biotransformation of castor oil into gamma-decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of gamma-decalactone of 6.5 g/l was obtained. The parameters of gamma-decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of gamma-decalactone for the three Macronet resins were linear. The trapping of gamma-decalactone produced by R. aurantiaca on these resins was then carried out. gamma-Decalactone was effectively retained by all the studied Macronet resins. The resin MN-202 trapped gamma-decalactone more efficiently than MN-102 and MN-100. The percentages of gamma-decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed gamma-decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract gamma-decalactone from culture broth of R. aurantiaca.
Alchihab
Mohamed
M
Centre Wallon de Biologie Industrielle, Gembloux, Belgium. alchihab.m@fsagx.ac.be
Aldric
Jean-Marc
JM
Aguedo
Mario
M
Destain
Jacqueline
J
Wathelet
Jean-Paul
JP
Thonart
Philippe
P
D000327:Adsorption; D003651:Decanoates / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D007783:Lactones / Q000302:isolation & purification* / Q000378:metabolism; D012117:Resins, Synthetic; D012248:Rhodotorula / Q000378:metabolism*
C548701:4-hydroxydecanoic acid; D003651:Decanoates; D007783:Lactones; D012117:Resins, Synthetic; C109757:decan-4-olide