title: Isolation and identification of a novel chlorophenol from a cell suspension culture of Helichrysum aureonitens.
abstract: A novel chlorophenol, 4-chloro-2-(hepta-1,3,5-triyn-1-yl)-phenol (1), was isolated as the major phenolic compound from the cells of Helichrysum aureonitens suspension cultures. Compound 1 has been proposed to be an intermediate in the acetylene biosynthetic pathway of other acetylenic compounds in Helichrysum spp. The ethanol extract of cell suspension cultures and compound 1 were evaluated for their cytotoxicity against monkey kidney Vero (Vero cells) and human prostate epithelial carcinoma (DU145) cell lines, also, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Mycobacterium tuberculosis H37Rv were determined as well.
authors.affiliation: Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Ziaratnia
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohyama
authors.forename: Ahmed Abdel-Fattah
authors.initials: AA
authors.lastname: Hussein
authors.forename: Toshiya
authors.initials: T
authors.lastname: Muranaka
authors.forename: Namrita
authors.initials: N
authors.lastname: Lall
authors.forename: Karl Josef
authors.initials: KJ
authors.lastname: Kunert
authors.forename: Jacobus Johannes Marion
authors.initials: JJ
authors.lastname: Meyer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry* / Q000166:cytology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D002733:Chlorophenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014709:Vero Cells
chemical_list: C546181:4-chloro-2-(hepta-1,3,5-triyn-1-yl)-phenol; D000970:Antineoplastic Agents; D000995:Antitubercular Agents; D002733:Chlorophenols; D010936:Plant Extracts