[Development of a suspension array method for quantitative detection of Y. pestis].
OBJECTIVE
To establish a platform for rapid, sensitive, specific, high-throughput, and simultaneous detection of multiple biothreat-associated agents, a suspension array-based immunoassay was developed for exploring the feasibility of directly simultaneous detection of Y. pestis in powder samples.


METHODS
The immunoassay using coupled fluorescent beads were employed to detect Y. pestis as a model from powder sample and the feasibility for detection in field samples was demonstrated by both blind and standard laboratory trials.


RESULTS
The newly developed suspension array appeared to be specific and sensitive, with the detection sensitivities of 0.154 ng/ml for Y. pestis F1 antigen, and the dynamic ranges of 0.154 - 4514 ng/ml, which were higher than those of corresponding conventional ELISA tests.


CONCLUSIONS
The suspension array could rapidly, sensitively, specifically and quantitatively detect pathogen from powder samples, which would be useful for early identification, rapid diagnosis, and response for the attack of bioterrorism and outbreak of infectious disease.
Wang
Jing
J
Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Wangjing0115@126.com
Shan
Linjun
L
Yang
Yongli
Y
Sun
Xiaohong
X
Yang
Yu
Y
Hu
Kongxin
K
Hou
Yousong
Y
Ding
Yanli
Y
D054045:Biological Warfare Agents; D007118:Immunoassay / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000235:genetics / Q000302:isolation & purification*
D054045:Biological Warfare Agents