Usefulness of addition of Orvus ES paste and sodium lauryl sulfate to frozen feline semen.
It has been shown that addition of the surfactant Orvus ES paste (OEP) and its main component sodium lauryl sulfate (SLS) to boar or dog semen before freezing improves post-thaw sperm motility and protects acrosome caps. In this study, we investigated the usefulness of the addition of OEP (0, 1, 2 and 4%) or SLS (0, 1, 2, 3 and 4 mg/ml) to cat ejaculates before freezing and their concentrations. Among the OEP addition groups, the 1% OEP group showed higher sperm motility than the other groups. Among the SLS addition groups, the 3 mg/ml SLS group showed slightly higher sperm motility and viability than the other groups. Comparison between the 1% OEP and 3 mg/ml SLS addition groups suggested a higher percentage of sperm with an acrosome cap in the 1% OEP group. The other sperm properties did not significantly differ between the 2 groups. These results indicate that addition of 1% OEP or 3 mg/ml SLS is effective for freezing of cat ejaculated semen.
Mizutani
Tatsuji
T
Department of Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan. tatsuji@mizutani-ah.com
Sumigama
Shiho
S
Nagakubo
Keiichi
K
Shimizu
Noriko
N
Oba
Hiromichi
H
Hori
Tatsuya
T
Tsutsui
Toshihiko
T
D000818:Animals; D002415:Cats; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa; D013501:Surface-Active Agents / Q000494:pharmacology*
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate