Toxicity of endophyte-infected tall fescue alkaloids and grass metabolites on Pratylenchus scribneri.
ABSTRACT Neotyphodium coenophialum, an endophytic fungus associated with tall fescue grass, enhances host fitness and imparts pest resistance. This symbiotum is implicated in the reduction of stresses, including plant-parasitic nematodes. To substantiate this implication, toxicological effects of root extracts, polyphenolic fraction, ergot, and loline alkaloids from endophyte-infected tall fescue were investigated using Pratylenchus scribneri, a nematode pest of tall fescue. In vitro bioassays and greenhouse studies were used as tests for effects of root fractions and compounds on motility and mortality of this lesion nematode. Greenhouse studies revealed that endophyte-infected tall fescue grasses are essentially nonhosts to P. scribneri, with root populations averaging 3 to 17 nematodes/pot, compared with 4,866 and 8,450 nematodes/pot for noninfected grasses. The in vitro assay indicated that root extracts from infected tall fescues were nematistatic. Polyphenols identified in extracts included chlorogenic acid, 3,5-dicaffeoylquinic acids, caffeic acid, and two unidentified compounds, but these were not correlated with endophyte status, qualitatively or quantitatively. Tests of several ergot alkaloids revealed that ergovaline and alpha-ergocryptine were nematicidal at 5 and 50 microg/ml, respectively, while ergocornine and ergonovine were nematistatic at most concentrations. Loline (N-formylloline), the pyrrolizidine alkaloid tested, was nematicidal (50 to 200 microg/ml). The ecological benefits of the metabolites tested here should assist in defining their role in deterring this nematode species while offering some probable mechanisms of action against plant-parasitic nematodes in general.
Bacetty
A A
AA
United States Department of Agriculture-Agricultural Reserach Services (USDA-ARS), Toxicology and Mycotoxin Research Unit, Richard Russell Center, Athens, GA, USA.
Snook
M E
ME
Glenn
A E
AE
Noe
J P
JP
Hill
N
N
Culbreath
A
A
Timper
P
P
Nagabhyru
P
P
Bacon
C W
CW
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004876:Ergot Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D031751:Festuca / Q000737:chemistry / Q000382:microbiology* / Q000469:parasitology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D055328:Neotyphodium / Q000254:growth & development*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry / Q000382:microbiology / Q000469:parasitology; D059808:Polyphenols; D013559:Symbiosis / Q000502:physiology; D017187:Tylenchida / Q000187:drug effects*
D000470:Alkaloids; D004876:Ergot Alkaloids; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; C059478:loline