title: Gender-linked haematopoietic and metabolic disturbances induced by a pesticide mixture administered at low dose to mice.
abstract: Defining the impact on health of exposure to a low-dose pesticide mixture via food intake is a topical question since epidemiological studies suggest that this may increase the risk of pathologies and particularly haematopoietic malignancies. Here we investigated on the haematopoietic system of mice, the effect of a mixture of six pesticides frequently ingested through the intake of fruits and vegetables produced in France (alachlor, captan, diazinon, endosulfan, maneb, mancozeb). The mixture was administered repeatedly by gavage to mice for 4 weeks at levels derived from the human Acceptable Daily Intake (ADI) level adapted to the mean weight of mice. Using a NMR-based metabonomic approach, we show that this treatment led to specific gender-linked variations in the level of hepatic metabolites involved in oxidative stress and in the regulation of glucose metabolism, indicating a metabolic signature for this repeated administration. Interestingly, exposure to the low-dose pesticide mixture induced significant changes in the blood cell counts with modifications in the clonogenic and differentiating capacities of haematopoietic progenitors showing abnormalities in the granulocytic and monocytic lineages in female and male mice, respectively. From a molecular point of view, the changes induced by the pesticide treatment correlated with modifications of the PI 3-kinase/Akt signalling pathway, the tyrosine kinase Pyk2 and the c-Myc transcription factor, which are involved in the balance between self-renewal and differentiation of haematopoietic stem cells. Our results point to a significant effect of a very low dose of a mixture of commonly used pesticides on mice metabolism and haematopoietic system with major differences between males and females.
authors.affiliation: UMR1089 Xénobiotiques, INRA, ENVT, F-31300 Toulouse, France. maysalounm@hotmail.com
authors.forename: M
authors.initials: M
authors.lastname: Merhi
authors.forename: C
authors.initials: C
authors.lastname: Demur
authors.forename: C
authors.initials: C
authors.lastname: Racaud-Sultan
authors.forename: J
authors.initials: J
authors.lastname: Bertrand
authors.forename: C
authors.initials: C
authors.lastname: Canlet
authors.forename: F Blas Y
authors.initials: FB
authors.lastname: Estrada
authors.forename: L
authors.initials: L
authors.lastname: Gamet-Payrastre
mesh_terms: D016573:Agrochemicals / Q000633:toxicity; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005260:Female; D006397:Hematinics / Q000633:toxicity*; D006410:Hematopoiesis / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010575:Pesticides / Q000633:toxicity*; D012737:Sex Factors
chemical_list: D016573:Agrochemicals; D015415:Biomarkers; D006397:Hematinics; D010575:Pesticides