title: Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells.
abstract: Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC(50) value of cimiside E on gastric cancer cells for 24 h was 14.58 microM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 microM) and G2/M phase at higher concentrations (60 and 90 microM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.
authors.forename: Lian Yu
authors.initials: LY
authors.lastname: Guo
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Joo
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jeon
authors.forename: Sehyun
authors.initials: S
authors.lastname: Jang
authors.forename: Eun Myoung
authors.initials: EM
authors.lastname: Shin
authors.forename: Hong Yu
authors.initials: HY
authors.lastname: Zhou
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031885:Cimicifuga / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D012503:Saponins; D014315:Triterpenes; C545903:cimiside E; D019014:fas Receptor