[N-stearoylethanolamine inhibits the proliferation of transformed cells and modulates mitochondrial enzyme activity in normal and transformed cells].
The N-stearoylethanolamine (NSE) influence on the proliferation of different cell types and the activity of mitochondrial electron transport enzymes, succinatedehydrogenase (SDG) and glycerophosphate-dehydrogenase (GFDG), in transformed cells under the action of NSE was studied. The incubation of the cells of mouse leukemic lymphocyte cell line L1210 and transformed mouse fibroblasts L929 with NSE caused the inhibition of cell growth and decreased the survival level of cells, but this effect was not associated with apoptotic cell death. It was shown for the first time that NSE addition to the cultural medium decreased the SDG activity and increased the GFDG activity in L929 cells. That leads to the SDG/GFDG imbalance in transformed fibroblasts and affects the cell energy metabolism. The results of the work suggest that N-stearoylethanolamine inhibited the transformed cell proliferation due to modulation of the activity of electron transport enzymes.
Khmel'
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Hula
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Asmolkova
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Lavrenchuk
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004579:Electron Transport; D004983:Ethanolamines / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D013229:Stearic Acids / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013997:Time Factors
D004983:Ethanolamines; C408368:N-stearoylethanolamine; D013229:Stearic Acids; D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase