Haloechinothrix alba gen. nov., sp. nov., a halophilic, filamentous actinomycete of the suborder Pseudonocardineae.
A novel halophilic, filamentous actinomycete strain, designated YIM 93221(T), was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. The isolate grew with 9-23 % (w/v) NaCl and did not grow without NaCl. The isolate formed spiny aerial mycelium and did not form spores at maturity. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and glucose, glucosamine, mannose and an unknown sugar as the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unknown phospholipid. MK-8(H(4)) was the predominant menaquinone. The major fatty acid was iso-C(16 : 0). The DNA G+C content was 68.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 93221(T) formed a distinct lineage within the suborder Pseudonocardineae and showed 91.9-94.8 % 16S rRNA gene sequence similarity with members of the suborder Pseudonocardineae. On the basis of the evidence from this polyphasic study, a novel genus and species, Haloechinothrix alba gen. nov., sp. nov., are proposed. The type strain of Haloechinothrix alba is YIM 93221(T) (=DSM 45207(T) =CCTCC AB 208140(T)).
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Wang
Yun
Y
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Zhang
Hua
H
The National Engineering Center for Research of Microbial Pharmaceuticals and New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Shijiazhuang 050015, PR China.
Lee
Jae-Chan
JC
Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea.
Lou
Kai
K
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Kim
Chang-Jin
CJ
Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride