Fitness study of the RDRio lineage and Latin American-Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil.
RD(Rio) is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) family. LAM has been found worldwide but is more predominant in South America. The aim of this study was to assess the presence of the RD(Rio) lineage and LAM family in the city of Rio Grande, Brazil, and to investigate the fitness of these strains based on determination of their growth rate. Fifty clinical isolates of M. tuberculosis were genotyped and 43 different patterns were found by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats. The predominant genotypes belonged to the LAM family (54% of the strains) followed by clade T (22%) and Haarlem (16%). The RD(Rio) lineage represented 38% of the total strains and 70.4% of the LAM strains found in this study. Strains belonging to the LAM family showed a fitness advantage when comparing their rate of growth with that of non-LAM strains, but a significant difference between RD(Rio) and non-RD(Rio) strains was not confirmed.
Von Groll
Andrea
A
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. avongroll@itg.be
Martin
Anandi
A
Felix
Carolina
C
Prata
Pedro Fernandes Sanmartin
PF
Honscha
Günther
G
Portaels
Françoise
F
Vandame
Peter
P
da Silva
Pedro Eduardo Almeida
PE
Palomino
Juan Carlos
JC
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D005260:Female; D056084:Genetic Fitness; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007843:Latin America; D008297:Male; D019083:Mediterranean Region; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult
D003470:Culture Media; D009841:Oligonucleotides