Detection of serum amyloid A isoforms in cattle.
Two-dimensional electrophoresis and immunoblotting were used to characterize 7 serum amyloid A (SAA) isoforms in cows with amyloidosis or chronic inflammation. Five SAA isoforms (isoelectric point [pI] 5.6, 6.1, 6.4, 6.8, and 7.7) were detected in all 10 amyloidosis serum samples, while 2 isoforms, pI 5.2 and 8.6, were detected in 4 and 9 of the samples, respectively. The same 7 isoforms were also detected in the serum of cows with chronic inflammation, but SAA pI 5.2 and 8.6 were detected in only 1 and 2 of 10 samples, respectively. It was concluded that although an amyloid-specific SAA isoform was not detected, examination of SAA isoform detection patterns may help identify bovine amyloidosis.
Takahashi
Eiji
E
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan.
Kuwayama
Hideto
H
Kawamoto
Keiko
K
Matsui
Takane
T
Inokuma
Hisashi
H
D000686:Amyloidosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D002417:Cattle / Q000097:blood*; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis; D007249:Inflammation / Q000097:blood / Q000175:diagnosis; D020033:Protein Isoforms / Q000097:blood*; D000685:Serum Amyloid A Protein / Q000032:analysis*
D020033:Protein Isoforms; D000685:Serum Amyloid A Protein