title: [Pre-treatment in determining total polysaccharide in flos Chrysanthemum indicum by response surface design].
abstract: OBJECTIVE
  To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici.
  
  
  METHODS
  The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design.
  
  
  RESULTS
  The best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best.
  
  
  CONCLUSIONS
  A model equation that can be used to predict the experiment is established through the response surface method.
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Fang
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Guo
authors.forename: Haijin
authors.initials: H
authors.lastname: Shen
mesh_terms: D053000:Analytic Sample Preparation Methods; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides