title: Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing.
abstract: A novel glucose biosensor based on immobilization of glucose oxidase in thin films of chitosan containing nanocomposites of graphene and gold nanoparticles (AuNPs) at a gold electrode was developed. The resulting graphene/AuNPs/chitosan composites film exhibited good electrocatalytical activity toward H(2)O(2) and O(2). The wide linear response to H(2)O(2) ranging from 0.2 to 4.2 mM (R=0.998) at -0.2V, high sensitivity of 99.5 microA mM(-1) cm(-2) and good reproducibility were obtained. The good electrocatalytical activity might be attributed to the synergistic effect of graphene and AuNPs. With glucose oxidase (GOD) as a model, the graphene/AuNPs/GOD/chitosan composite-modified electrode was constructed through a simple casting method. The resulting biosensor exhibited good amperometric response to glucose with linear range from 2 to 10 mM (R=0.999) at -0.2V and from 2 to 14 mM (R=0.999) at 0.5 V, good reproducibility and detection limit of 180 microM. Glucose concentration in human blood was studied preliminarily. From 2.5 to 7.5 mM, the cathodic peak currents of the biosensor decrease linearly with increasing the glucose concentrations. The graphene/AuNPs/GOD/chitosan composites film shows prominent electrochemical response to glucose, which makes a promising application for electrochemical detection of glucose.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate University of the Chinese Academy of Sciences, Changchun, PR China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Shan
authors.forename: Huafeng
authors.initials: H
authors.lastname: Yang
authors.forename: Dongxue
authors.initials: D
authors.lastname: Han
authors.forename: Qixian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ari
authors.initials: A
authors.lastname: Ivaska
authors.forename: Li
authors.initials: L
authors.lastname: Niu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose