title: Tailoring the soliton output of a photonic crystal fiber for enhanced two-photon excited luminescence response from fluorescent protein biomarkers and neuron activity reporters.
abstract: Dual-cladding photonic crystal fibers (PCFs) with two zero-dispersion points are used to enhance the two-photon excited luminescence (TPL) response from fluorescent protein biomarkers and neuron activity reporters in dye-cell experiments and in in vivo work on transgenic mice and tadpoles. The soliton transmission of ultrashort pulses through a PCF suppresses dispersion-induced temporal pulse spreading, maintaining a high level of field intensity needed for efficient TPL excitation. The soliton self-frequency shift, stabilized against laser power fluctuations by a specific PCF dispersion design, is employed to accurately match the wavelength of the soliton PCF output with the two-photon absorption spectrum of dye or fluorescent protein biomarker molecules, enhancing their TPL response and allowing the laser damage of biotissues to be avoided.
authors.affiliation: Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Russia.
authors.forename: Lyubov V
authors.initials: LV
authors.lastname: Doronina
authors.forename: Il'ya V
authors.initials: IV
authors.lastname: Fedotov
authors.forename: Aleksandr A
authors.initials: AA
authors.lastname: Voronin
authors.forename: Olga I
authors.initials: OI
authors.lastname: Ivashkina
authors.forename: Marina A
authors.initials: MA
authors.lastname: Zots
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Anokhin
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Rostova
authors.forename: Andrei B
authors.initials: AB
authors.lastname: Fedotov
authors.forename: Aleksei M
authors.initials: AM
authors.lastname: Zheltikov
mesh_terms: D000042:Absorption; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D007834:Lasers; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D017785:Photons; D013050:Spectrometry, Fluorescence
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins