title: SKAP1 is dispensable for chemokine-induced migration of primary T-cells.
abstract: Immune adaptors SLP-76, ADAP and SKAP1 (SKAP-55) play central roles in anti-CD3 induced 'inside-out' signalling for LFA-1 activation and ICAM-1 adhesion. However, it has been unclear whether SKAP1 is also required for chemokine-induced T-cell motility. In this study, we found that SDF-1 and CCL21 induced similar motility in SKAP1 deficient (SKAP1-/-) and wild type (SKAP1+/+) resting, primary T-cells. In addition, the speed (i.e. 13 microM/min), tracking distance (i.e. length) and displacement values (i.e. direct distance between the start and the end positions of cell movement) in response to SDF1 were similar for SKAP1-/- and SKAP1+/+ primary, activated T-cells. Relatively high strength anti-CD3 ligation also arrested the migration (i.e. stop-signal) of resting SKAP1+/+ and SKAP1-/- T-cells in the presence of SDF-1 and CCL21. These data demonstrate that contrary to its central role in anti-CD3 induced LFA-1 adhesion, the response of primary T-cells to SDF-1 and CCL21 is not profoundly dependent on SKAP1 expression.
authors.affiliation: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wang
authors.forename: Yuning
authors.initials: Y
authors.lastname: Lu
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Rudd
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054421:Chemokine CCL21 / Q000276:immunology / Q000494:pharmacology; D054377:Chemokine CXCL12 / Q000276:immunology / Q000494:pharmacology; D018925:Chemokines; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D051379:Mice; D010750:Phosphoproteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
chemical_list: D054421:Chemokine CCL21; D054377:Chemokine CXCL12; D018925:Chemokines; D010750:Phosphoproteins