Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder.
Single-prolonged stress (SPS) is an established animal model for post-traumatic stress disorder (PTSD). The calcium (Ca2+)-calmodulin (CaM)-CaM kinase (K)IIalpha signal passage plays an important role in the plasticity of central nervous, learning and memory, mind and behavior and other types of cognitive activities. The amygdala is known to play an important role in fear, rage and emotional memory. In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly increased, and CaMKIIalpha expression significantly decreased (P<0.05) in the basolateral amygdala after SPS. These findings suggest dysfunction of Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of SPS rats, which may play important roles in the pathogenesis of PTSD rats.
Xiao
Bing
B
Department of Histology and Embryology, Basic Medical Sciences College, China Medical University, Shenyang 110001, PR China.
Han
Fang
F
Shi
Yu-Xiu
YX
D000679:Amygdala / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007424:Intracellular Fluid / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013313:Stress Disorders, Post-Traumatic / Q000201:enzymology*; D013312:Stress, Physiological / Q000502:physiology; D013315:Stress, Psychological / Q000378:metabolism
D002147:Calmodulin; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium