title: A new single-platform method for the enumeration of CD34+ cells.
abstract: Guidelines for flow cytometric enumeration of CD34(+) hematopoietic stem cells (HSC) recommend the use of a single-platform assay. The SCE kit has recently been commercialized by BD Biosciences. Results obtained with this newly available kit were compared with CD34(+) cell enumerations obtained in parallel with already commercialized diagnostic kits; fresh peripheral blood, apheresis, cord blood (CB) and bone marrow (BM) samples, as well as thawed apheresis and CB samples, were assayed. The SCE kit produced data for CD34(+) enumeration that correlate well with data produced with the older assays (r(2) > or = 0.9). Practical advantages were the ability to enumerate viable CD34 cells in all kinds of HSC products, the absence of bead pipetting (which decreases results precision) and a gating strategy complying with international recommendations. A major disadvantage was the absence of specific software for data analyses and presentation of results.
authors.affiliation: Centre de Thérapie Cellulaire et Génique, Institut Paoli-Calmettes, Marseille, France. lemariec@marseille.fnclcc.fr
authors.forename: Claude
authors.initials: C
authors.lastname: Lemarie
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bouchet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sielleur
authors.forename: Véronique
authors.initials: V
authors.lastname: Durand
authors.forename: Florent
authors.initials: F
authors.lastname: Navarro
authors.forename: Boris
authors.initials: B
authors.lastname: Calmels
authors.forename: Christian
authors.initials: C
authors.lastname: Chabannon
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002452:Cell Count / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D011933:Reagent Kits, Diagnostic
chemical_list: D018952:Antigens, CD34; D011933:Reagent Kits, Diagnostic