Changed profile of splicing regulator genes expression in response to exercise.
Intensive exercise triggers the cascade processes of body adaptation, including modulation of splisosome functioning, and can lead to modification of its activity and choice of alternative exons. We studied the effect of exercise of the maximum aerobic power on activation of transcription of genes involved in the splicing process. Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: DDX17, DDX46, HNRNPR, PRPF4B, and SRPK2. The role of the detected regulators in initiation of splisosome assembly under conditions of maximally intensive exercise is discussed.
Tonevitsky
E A
EA
All-Russian Institute of Physical Culture and Athletics, Moscow, Russia.
Trushkin
E V
EV
Shkurnikov
M U
MU
Akimov
E B
EB
Sakharov
D A
DA
D000293:Adolescent; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology; D012326:RNA Splicing / Q000235:genetics*; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000235:genetics / Q000502:physiology; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000235:genetics / Q000502:physiology; D055815:Young Adult
C439930:HNRNPR protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D017413:Ribonucleoprotein, U2 Small Nuclear; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; C489984:PRPF4B protein, human; D017346:Protein Serine-Threonine Kinases; C110522:SRPK2 protein, human; C102004:DDX17 protein, human; C467585:DDX46 protein, human; D053487:DEAD-box RNA Helicases