[The effect of gallic acid extracted from leaves of Phyllanthus emblica on apoptosis of human hepatocellular carcinoma BEL-7404 cells].
OBJECTIVE
To explore the effect of gallic acid extracted from Leaves of Phyllanthus emblica on the apoptosis of BEL-7404 cells.


METHODS
MTT assay was applied to detect the influence on prolifetation in vitro. Inverted microscope was utilized to observe the morphological changes after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method was used to detect earlier period apoptosis cells and Tunel was applied to calculate the apoptosis rates.


RESULTS
Gallic acid could restrain the BEL-7404 cells proliferation at diffierent levels in a time and concentration dependent manner. The typical morphological changes of apoptosis were observed after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method and Tunel method showed that the viable apoptotic cell and apoptosis rates added as action time prolonged.


CONCLUSIONS
Gallic acid can restrain the BEL-7404 cells proliferation and induce apoptosis, and its effect on apoptosis is time dependent.
Zhong
Zhen-Guo
ZG
Guangxi Traditional Chinese Medical University, Nanning 530001, China. zhongzg@gxtcmu.edu.cn
Huang
Jin-Lan
JL
Liang
Hong
H
Zhong
Yi-Ning
YN
Zhang
Wen-Yan
WY
Wu
Deng-Pan
DP
Zeng
Chun-Lan
CL
Wang
Jin-Sheng
JS
Wei
Yong-Han
YH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D029842:Phyllanthus emblica / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013997:Time Factors
D000972:Antineoplastic Agents, Phytogenic; D005707:Gallic Acid