title: Short communication: Methamphetamine treatment increases in vitro and in vivo HIV replication.
abstract: To delineate the mechanistic basis for the epidemiological association between methamphetamine use and accelerated progression to AIDS, we evaluated the direct in vitro and in vivo effects of methamphetamine on HIV-1 replication. Methamphetamine administration significantly increased HIV-1 production by both HIV-infected monocytes and CD4 T lymphocytes in vitro. In addition, in vivo methamphetamine treatment increased HIV production and viremia in mice transgenic for a replication-competent HIV provirus and human cyclin T1. Methamphetamine activated transcription of the HIV long terminal repeat (LTR) regulatory region, was associated with nuclear translocation of NF-kappaB. Our results provide further insights into the mechanisms by which methamphetamine accelerates disease course in HIV-infected individuals.
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: Sima Shelly
authors.initials: SS
authors.lastname: Toussi
authors.forename: Aviva
authors.initials: A
authors.lastname: Joseph
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Zheng
authors.forename: Monica
authors.initials: M
authors.lastname: Dutta
authors.forename: Laura
authors.initials: L
authors.lastname: Santambrogio
authors.forename: Harris
authors.initials: H
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000821:virology; D002478:Cells, Cultured; D056906:Cyclin T / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D016325:HIV Long Terminal Repeat / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000821:virology; D008694:Methamphetamine / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D014766:Viremia / Q000188:drug therapy / Q000821:virology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D056906:Cyclin T; D008694:Methamphetamine