Further development of raccoon poxvirus-vectored vaccines against plague (Yersinia pestis).
In previous studies, we demonstrated protection against plague in mice and prairie dogs using a raccoon pox (RCN) virus-vectored vaccine that expressed the F1 capsular antigen of Yersinia pestis. In order to improve vaccine efficacy, we have now constructed additional RCN-plague vaccines containing two different forms of the lcrV (V) gene, including full-length (Vfull) and a truncated form (V307). Mouse challenge studies with Y. pestis strain CO92 showed that vaccination with a combination of RCN-F1 and the truncated V construct (RCN-V307) provided the greatest improvement (P=0.01) in protection against plague over vaccination with RCN-F1 alone. This effect was mediated primarily by anti-F1 and anti-V antibodies and both contributed independently to increased survival of vaccinated mice.
Rocke
Tonie E
TE
National Wildlife Health Center, USGS/BRD, 6006 Schroeder Rd., Madison, WI 53711, United States. trocke@usgs.gov
Iams
Keith P
KP
Dawe
Sandra
S
Smith
Susan R
SR
Williamson
Judy L
JL
Heisey
Dennis M
DM
Osorio
Jorge E
JE
D000818:Animals; D016001:Confidence Intervals; D051379:Mice; D017992:Parvovirus / Q000235:genetics*; D010930:Plague / Q000517:prevention & control; D010931:Plague Vaccine / Q000235:genetics / Q000276:immunology*; D016016:Proportional Hazards Models; D015010:Yersinia pestis / Q000276:immunology*
D010931:Plague Vaccine