title: Inhibition of electrocatalytic O(2) reduction of functional CcO models by competitive, non-competitive, and mixed inhibitors.
abstract: Electrocatalytic reduction of O(2) by functional cytochrome C Oxidase (CcO) models is studied in the presence of several known inhibitors like CO, N(3)(-), CN(-), and NO(2)(-). These models successfully reproduce the inhibitions observed in CcO at similar concentrations reported for these inhibitors. Importantly, the data show very different electrochemical responses depending on the nature of the inhibitor, that is, competitive, non-competitive and mixed. Chemical models have been provided for these observed differences in the electrochemical behavior. Using the benchmark electrochemical behaviors for known inhibitors, the inhibition by NO(2)(-) is investigated. Electrochemical data suggests that NO(2)(-) acts as a competitive inhibitor at high concentrations. Spectroscopic data suggests that NO released during oxidation of the reduced catalyst in presence of excess NO(2)(-) is the source of the competitive inhibition by NO(2)(-). Presence of the distal Cu(B) lowers the inhibitory effect of CN(-) and NO(2)(-). While for CN(-) it weakens its binding affinity to the reduced complex by approximately 4.5 times, for NO(2)(-), it allows regeneration of the active catalyst from a catalytically inactive, air stable ferrous nitrosyl complex via a proposed superoxide mediated pathway.
authors.affiliation: jpc@stanford.edu
authors.forename: James P
authors.initials: JP
authors.lastname: Collman
authors.forename: Abhishek
authors.initials: A
authors.lastname: Dey
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Barile
authors.forename: Somdatta
authors.initials: S
authors.lastname: Ghosh
authors.forename: Richard A
authors.initials: RA
authors.lastname: Decréau
mesh_terms: D001667:Binding, Competitive; D055162:Biocatalysis; D020134:Catalytic Domain; D003300:Copper / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D008024:Ligands; D006108:Graphite; D003300:Copper; D007501:Iron; D003576:Electron Transport Complex IV; D010100:Oxygen