Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis.
Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling.
Mashiguchi
Kiyoshi
K
Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan. k.mashiguchi@psc.riken.jp
Sasaki
Eriko
E
Shimada
Yukihisa
Y
Nagae
Miyu
M
Ueno
Kotomi
K
Nakano
Takeshi
T
Yoneyama
Koichi
K
Suzuki
Yoshihito
Y
Asami
Tadao
T
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics; D025461:Feedback, Physiological / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007783:Lactones / Q000378:metabolism* / Q000494:pharmacology*; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects
D029681:Arabidopsis Proteins; D007783:Lactones