title: The role of glucocorticoid in SIRP alpha and SHP-1 gene expression in AIHA patients.
abstract: The aim of this work was to evaluate the regulation of SIRP alpha, an inhibitory phagocyte receptor, and the phosphatase SHP-1 in monocytes of patients with autoimmune hemolytic anemia, and the role of dexamethasone on SIRP alpha and SHP-1 gene expression and erythrophagocytosis in vitro. SIRP alpha and SHP-1 expression was higher in monocytes from AIHA patients compared with normal, returning to normal after glucocorticoid therapy. SIRP alpha and SHP-1 mRNA expression was upregulated in healthy monocytes treated with dexamethasone compared with basal; however, the erythrophagocytic ability was not altered. Our results point to a minor role of SIRP alpha and SHP-1 in determining AIHA.
authors.affiliation: Center for Investigation in Pediatrics and Department of Pharmacology, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: de Almeida
authors.forename: Soraya Massaro
authors.initials: SM
authors.lastname: Barbosa
authors.forename: Maria de Lourdes
authors.initials: Mde L
authors.lastname: Barjas-Castro
authors.forename: Sara Teresinha
authors.initials: ST
authors.lastname: Olalla-Saad
authors.forename: Antonio
authors.initials: A
authors.lastname: Condino-Neto
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000235:genetics / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000276:immunology*; D005938:Glucocorticoids / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D000943:Antigens, Differentiation; D005938:Glucocorticoids; D011971:Receptors, Immunologic; C501920:SIRPA protein, human; D003907:Dexamethasone; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6