title: Influence of suture regularity on corneal astigmatism after penetrating keratoplasty.
abstract: OBJECTIVE
  To investigate whether suture regularity affects corneal astigmatism after keratoplasty.
  
  
  METHODS
  Twenty-one patients undergoing penetrating keratoplasty for various corneal diseases were included in the study. The grafts were sutured in place using a single-running Nylon 10-0 suture, taking 24 bites. Immediately after surgery, standard calibrated images of the grafted eye were captured and stored. Using a dedicated image analysis programme, stitches and needle points were identified, and a number of suture regularity variables were calculated. Corneal topographic images were obtained before suture removal (12 months after surgery) and 3 months after suture removal (18 months after surgery). Topographic measures of astigmatism [surface regularity (SRI), surface asymmetry index (SAI) and simulated keratometric astigmatism] were calculated and correlated with the computed suture regularity variables.
  
  
  RESULTS
  The average stitch length was 3.04 ± 0.28 mm and the distance between the outer needle points was 2.53 ± 0.09 mm. The SRI was 1.26 ± 0.36 and the SAI was 1.59 ± 0.67 after 12 months; these decreased to 1.03 ± 0.48 and 0.92 ± 0.46 after 18 months, respectively. Corneal astigmatism was 6.38 ± 2.99 and 5.87 ± 3.13 dioptres after 12 and 18 months, respectively. Suture regularity did not affect SAI, SRI or corneal astigmatism significantly 12 months after surgery. Eighteen months after surgery (3 months after suture removal), the standard deviation on the original stitch length was found to significantly increase corneal astigmatism. In addition, the size of the counter-clockwise angle between stitch and graft radian was correlated significantly with a lower SRI.
  
  
  CONCLUSIONS
  The origin of corneal astigmatism after penetrating keratoplasty is multifaceted. Regular stitch length and stitch advancement on the surface appears to improve the optical quality of the graft after suture removal. Factors such as stitch depth, suture tension and variations in wound construction might also be important predictors of corneal astigmatism after penetrating keratoplasty.
authors.affiliation: Department of Opthalmology, Aarhus University Hospital, Denmark. jesper.hjortdal@dadlnet.dk
authors.forename: Jesper
authors.initials: J
authors.lastname: Hjortdal
authors.forename: Anders
authors.initials: A
authors.lastname: Søndergaard
authors.forename: Walther
authors.initials: W
authors.lastname: Fledelius
authors.forename: Niels
authors.initials: N
authors.lastname: Ehlers
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001251:Astigmatism / Q000175:diagnosis / Q000209:etiology* / Q000517:prevention & control; D019781:Corneal Topography; D005260:Female; D005500:Follow-Up Studies; D005642:Fuchs' Endothelial Dystrophy / Q000601:surgery; D006801:Humans; D007640:Keratoconus / Q000601:surgery*; D015948:Keratoplasty, Penetrating / Q000009:adverse effects* / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000175:diagnosis / Q000209:etiology* / Q000517:prevention & control; D013536:Suture Techniques; D055815:Young Adult