Effect of butaphosphane and cyanocobalamin on regeneration of muscle fibres in pigs.
The aim of the study was to monitor the effect of butaphosphane (1-(n-butylamino)-1-methylophosphorous acid) and cyanocobalamin (Catosal preparation, Bayer AG) on regeneration of the longissimus lumborum muscle (musculus longissimus lumborum) in pigs. Experiments were conducted on 34 piglets of Polish Large White breed with a mean body weight of 20 kg that were divided into two groups. Piglets of group I (control) received an intramuscular injection of 10 cm3 of 0.5% bupivacaine hydrochloride at both side of the spine. Piglets of group II were injected with bupivacaine, as in group I, and additionally received intramuscular injections of 5 ml of Catosal for 5 subsequent days. The animals were euthanized 6, 12, and 24 hours as well as 2, 3, 4, 5, 7, 10 and 14 days after muscle injury. Preparations obtained from muscle specimens were stained with HE, PAS method acc. to McManus, HBFP, Feulgen, and Unna methods. Ultrastructural preparations (TEM) were prepared following a standard procedure. The presence of vimentin, desmin and PCNA was detected immunohistochemically in sections prepared with a paraffin method. Necrosis of muscle fibres was observed in all animals after bupivacaine injection. The administration of Catosal accelerated the regeneration of damaged skeletal muscles in pigs through the facilitation of phagocytosis and enhancement of myogenic cells proliferation. No effect of Catosal was found on differentiation of myoblasts or maturation of newly-formed muscle fibres.
Otrocka-Domagała
I
I
Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland. i.otrocka-domagala@uwm.edu.pl
Rotkiewicz
T
T
Podbielski
M
M
Wiśniewska
A
A
Drzewiecka
A
A
D000818:Animals; D002045:Bupivacaine / Q000009:adverse effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects*; D009135:Muscular Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000662:veterinary*; D009336:Necrosis / Q000139:chemically induced / Q000188:drug therapy / Q000662:veterinary*; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced / Q000188:drug therapy*; D014805:Vitamin B 12 / Q000494:pharmacology*
D063065:Organophosphonates; D009943:Organophosphorus Compounds; C030504:butaphosphone; D014805:Vitamin B 12; D002045:Bupivacaine