title: An inexpensive technique for dot blotting in a 96-well format.
abstract: Dot blotting for the quantification of proteins usually requires expensive devices. Here we present an inexpensive way to facilitate dot blotting in a 96-well format. An agarose gel was used as sample reservoir, and proteins were electrophoretically transferred from the gel to a membrane. Dots produced by this technique were applied to reproducible immunoquantification of proteins such as beta-actin and doublecortin.
authors.affiliation: Paul Flechsig Institute for Brain Research, University of Leipzig, D-04109 Leipzig, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Lange
authors.forename: Thomas
authors.initials: T
authors.lastname: Arendt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Härtig
mesh_terms: D000199:Actins / Q000032:analysis*; D000089622:Doublecortin Domain Proteins; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D009479:Neuropeptides / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000199:Actins; D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D012967:Sodium Dodecyl Sulfate