title: A functional polymorphism in Pre-miR-146a gene is associated with prostate cancer risk and mature miR-146a expression in vivo.
abstract: BACKGROUND
  A G > C polymorphism (rs2910164) which is located in the sequence of miR-146a precursor, results in a change from a G:U pair to a C:U mismatch in its stem region. To explore whether rs2910164 plays any role in prostate cancer (CaP), we analyzed the association between miR-146a polymorphism and risk of CaP and the expression of miR-146a with different genotypes in CaP tissues in southern Chinese Han population.
  
  
  METHODS
  Two hundred fifty-one CaP and 280 control subjects were included in the cancer association study, and 15 CaP tissue samples were used to test the expression of the miRNA precursors by real-time quantitative reverse transcription PCR.
  
  
  RESULTS
  We found that subjects carrying CC homozygotes had a 0.65-fold reduced risk (95% CI = 0.43-0.99) than those carrying GG/GC genotypes (P = 0.03), and the C allele displayed a lower prevalence of CaP compared with the G allele (OR = 0.73, 95% CI = 0.57-0.94, P = 0.01). Moreover, hsa-miR-146a quantification showed that homozygous carriers of the C-variant had significantly decreased miRNA levels compared to the carriers of the GG/GC genotype.
  
  
  CONCLUSIONS
  The natural genetic variation in pre-miR-146a affects the amount of mature miR-146a, contributes to the genetic predisposition to CaP.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Ning-Han
authors.initials: NH
authors.lastname: Feng
authors.forename: Peng-Chao
authors.initials: PC
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.forename: Deyao
authors.initials: D
authors.lastname: Wu
authors.forename: Zheng-Dong
authors.initials: ZD
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Tong
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Ning-Hong
authors.initials: NH
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Hua
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms / Q000209:etiology / Q000235:genetics*; D012306:Risk
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs