Mass spectrometric analysis of peptides in brain neurosecretory cells and neurohemal organs in the adult blowfly, Protophormia terraenovae.
Neuropeptides in neurosecretory cells of the pars intercerebralis (PI) and pars lateralis (PL) in the brain, and those in the corpus cardiacum-hypocerebral ganglion complex (CC-HG) and corpus allatum (CA) were examined by mass spectrometry and immunocytochemistry in adult females of the blowfly, Protophormia terraenovae. By using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and electrospray ionization quadrupole orthogonal acceleration time-of-flight mass spectrometry (ESI-Q-Tof MS) and MS/MS, 4 peptides (including myosuppressin and SIFamide) were detected in the PI, 12 peptides (including [Arg(7)]-corazonin and [Arg(7)]-corazonin(3)(-)(11)) in the PL, 13 peptides (including myosuppressin, [Arg(7)]-corazonin and [Arg(7)]-corazonin(3-11)) in the CC-HG, and 6 peptides in the CA. MALDI-TOF MS analysis of each tissue or organ was made in about 20 flies under diapause-inducing (LD 12:12 at 20 degrees C) and diapause-averting conditions (LD 18:6 at 25 degrees C). These molecular ion peaks did not distinctively differ between diapause-inducing and diapause-averting conditions. A peptide with an m/z value at 1395.1 was purified from 240 brains and the 2nd-10th amino acids were sequenced as -YRKPPFNGS-, corresponding to a partial sequence of SIFamide. Only two pairs of somata in the PI were immunoreactive to antisera against SIFamide, which were local neurons widely extending fibers throughout the brain neuropils.
Inosaki
Aoi
A
Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
Yasuda
Akikazu
A
Shinada
Tetsuro
T
Ohfune
Yasufumi
Y
Numata
Hideharu
H
Shiga
Sakiko
S
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D003335:Corpora Allata / Q000737:chemistry / Q000166:cytology; D004175:Diptera / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis*; D009490:Neurosecretory Systems / Q000737:chemistry* / Q000166:cytology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019476:Insect Proteins; D009479:Neuropeptides; C060602:corazonin protein, insect