title: Intergeneric somatic cell nucleus transfer in marbled cat and flat-headed cat.
abstract: Intergeneric nucleus transfer (ig-NT) is a promising technique to produce offspring of endangered species. The objectives of this study were to (1) investigate the in vitro development of marbled cat (MC; Pardofelis marmorata) and flat-headed cat (FC; Prionailurus planiceps) ig-NT embryos reconstructed from domestic cat (DC; Felis catus) oocytes (Experiment 1), (2) evaluate the effect of individual FC donor cell lines on NT success (Experiment 2), and (3) assess the developmental ability of FC-cloned and DC-IVF embryos in vitro and in vivo after oviductal transfer (Experiment 3). In Experiment 1, the morula rate of FC-reconstructed embryos was significantly higher than those of MC and DC embryos but lower than that of parthenogenic DC embryos. However, blastocyst rate was not different. In Experiment 2, FC-ig-NT embryos reconstructed from female muscular tissue had significantly higher morula rate in comparison with those derived from other donor cell lines. However, there was no difference in blastocyst rate among cell lines. In Experiment 3, in vitro development of FC-ig-NT embryos was lower than that of DC-IVF embryos. The competency of in vivo development of FC-ig-NT and/or DC-IVF embryos was investigated by assessing pregnancy rate after their transfer into DC recipients. Domestic cat recipients receiving only FC-ig-NT embryos, FC-ig-NT embryos in one side of the oviduct and DC-IVF embryos contralaterally (co-transfer), and only DC-IVF embryos were observed. No pregnancy was detected in all recipients receiving FC-ig-NT embryos. One recipient receiving co-transferred embryos became pregnant, then delivered DC-IVF dead fetuses (n=2) and live kittens (n=6). All recipients receiving DC-IVF embryos became pregnant, and three of six recipients delivered five DC-IVF kittens. These results illustrate the developmental capacity of MC- and FC-ig-NT embryos up to the blastocyst stage. Individual donor cell line affects the developmental success up to the morula stage of FC-ig-NT embryos. Improving the developmental competence and quality of FC-ig-NT embryos may be required for implantation and development to term of FC-ig-NT offspring.
authors.affiliation: Zoological Park Organization under the Royal Patronage of H.M. the King, Bangkok, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Thongphakdee
authors.forename: B
authors.initials: B
authors.lastname: Siriaroonrat
authors.forename: S
authors.initials: S
authors.lastname: Manee-in
authors.forename: N
authors.initials: N
authors.lastname: Klincumhom
authors.forename: S
authors.initials: S
authors.lastname: Kamolnorranath
authors.forename: K
authors.initials: K
authors.lastname: Chatdarong
authors.forename: M
authors.initials: M
authors.lastname: Techakumphu
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D019976:Cloning, Organism / Q000379:methods; D004624:Embryo Transfer; D047108:Embryonic Development; D056727:Endangered Species; D045989:Felidae; D005260:Female; D005307:Fertilization in Vitro; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary