[Effect of 17beta-estradiol on mRNA expression of Bax proapoptotic protein and DNA fragmentation level in the human adrenal cortex].
The effect of 17beta-estradiol on the change of proapoptotic protein Bax mRNA level and DNA laddering in human adrenal cortex tissue was studied. The adding of 17beta-estradiol to incubation medium caused a decrease of the proapoptotic protein Bax mRNA level in adrenocorticocytes by 22% in comparison with control. Hormone influence decreases the DNA fragmentation by 50%. The obtained data suggest that 17beta-estradiol caused a significant antiapoptotic effect in human adrenal cortex.
Tron'ko
M D
MD
Kovzun
O I
OI
Hrinchenko
Ie M
IeM
Mykosha
O S
OS
D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D046509:Tissue Culture Techniques; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
C494826:BAX protein, human; D004967:Estrogens; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D004958:Estradiol