title: Use of oligonucleotides conjugated to gold nanoparticles and streptavidin for amplification of optical biosensor signal during detection of telomeric repeats.
abstract: Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied by the surface plasmon resonance method. Conjugation of the probe with streptavidin and gold nanoparticles was shown to amplify the signal at similar concentrations of this probe (by 60 and 300 times, respectively). Nanoparticles can be used for biosensor signal amplification in studying the telomerase activity of malignant cells.
authors.affiliation: V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Rad'ko
authors.forename: S A
authors.initials: SA
authors.lastname: Voronina
authors.forename: A V
authors.initials: AV
authors.lastname: Gromov
authors.forename: O V
authors.initials: OV
authors.lastname: Gnedenko
authors.forename: N V
authors.initials: NV
authors.lastname: Bodoev
authors.forename: A S
authors.initials: AS
authors.lastname: Ivanov
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000737:chemistry* / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D006046:Gold; D019809:Streptavidin; D019098:Telomerase