[Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus].
The chemical constituents of Leonurus heterophyllus were separated and purified by repeated column chromatography on silica gel, HPD 100, Sephadex LH-20, and PHPLC. Each compound was characterized by spectroscopic and physical data. Eight compounds have been purified and identified to be quercetin 3-O-robinobioside (1), rutin (2), isoquerci trin (3), hyperoside (4), quercetin (5), apigenin (6), genkwanin (7), and benzoic acid (8). Among them, compounds 2, 5-7 were isolated from L. heterophyllus for the first time; Compounds 1, 3, 4, 8 were obtained for the first time from the genus Leonurus. The in vitro activities against leukemia K562 Cells of pure components were evaluated by testing their IC50. Compounds 1-6, 8 exhibited in-vitro inhibitory activities against leukemia K562 cells in different extent.
Cong
Yue
Y
Institute of Traditional Chinese Materia Medica, School of Pharmacy, Henan University, Kaifeng 475004, China.
Guo
Jinggong
J
Wang
Tianxiao
T
Li
Mian
M
Li
Ke
K
Wang
Jinhui
J
Li
Qin
Q
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D031332:Leonurus / Q000737:chemistry*
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal