title: The growth factor independence-1 (Gfi1) is overexpressed in chronic myelogenous leukemia.
abstract: The activation of ABL tyrosine kinase in BCR/ABL-positive chronic myelogenous leukemia (CML) leads to a diversity of biological changes related to the pathogenesis of the disease. In CML patients, we determined the expression of growth factor independence-1 (Gfi1), a transcription repressor with weak oncogenic activity. Our data demonstrated that the Gfi1 mRNA level in both the mononuclear cells and purified CD34(+) cells from CML were significantly higher as measured by quantitative real-time PCR. Using flow cytometry and Western blot, we also showed that the Gfi1 protein content was increased in CML CD34(+) cells. The expression of Gfi1 was correlated with BCR/ABL significantly. Gfi1 may be implicated in the pathogenesis of CML and can serve as a potential target for the management of the disease.
authors.affiliation: Department of Hematology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Chang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Ou
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001483:Base Sequence; D001549:Benzamides; D001752:Blast Crisis / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D016313:Genes, abl; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D000068877:Imatinib Mesylate; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D015465:Leukemia, Myeloid, Accelerated Phase / Q000235:genetics / Q000378:metabolism; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000378:metabolism; D010879:Piperazines / Q000627:therapeutic use; D011743:Pyrimidines / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D033581:Stem Cell Transplantation; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D001549:Benzamides; D017931:DNA Primers; D004268:DNA-Binding Proteins; C498275:GFI1 protein, human; D010879:Piperazines; D011743:Pyrimidines; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors; D000068877:Imatinib Mesylate