title: Biotinylated multivalent glycoconjugates for surface coating.
abstract: Systematic studying of biological processes driven by multipoint high-cooperative carbohydrate recognition requires application of multivalent carbohydrates as tools. In this regard polyacrylamides with various pendant carbohydrate residues and labels are probably the most well advanced class of carbohydrate multimerics. Here we describe a synthetic approach to polyacrylamide-based glycoconjugates with biotin tag, with special emphasis to development of carbohydrate biosensors and arrays.
authors.affiliation: M.M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Chinarev
authors.forename: Oxana E
authors.initials: OE
authors.lastname: Galanina
authors.forename: Nicolai V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001671:Biochemistry / Q000379:methods; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002241:Carbohydrates / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006001:Glycoconjugates / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D051379:Mice; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D012997:Solvents; D013499:Surface Properties; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001710:Biotin; D002241:Carbohydrates; D006001:Glycoconjugates; D006023:Glycoproteins; D011108:Polymers; D012997:Solvents; D000099166:Polyacrylamides