title: The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin.
abstract: In this study, the vacuole disruptive activity was evaluated as a cause of amphotericin B (AmB) lethality against the pathogenic fungus Candida albicans in terms of its enhancement by allicin, an allyl-sulfur compound from garlic. Vacuole disruption was observed in parallel to AmB-induced cell death when the antibiotic was used at a lethal concentration and at a non-lethal concentration in combination with allicin. Allicin did not enhance AmB-induced cell death and the accompanying vacuole disruption when the cells were incubated with exogenous ergosterol for its enrichment in the vacuole. The vacuoles isolated from intact cells could be directly disrupted by the action of AmB to the same extent in the absence and presence of allicin, whereas the organelles isolated from ergosterol-enriched cells were resistant to its direct disruptive action. AmB was similarly incorporated into the fungal cytoplasm in cells with or without ergosterol enrichment, supporting the fact that AmB-induced vacuole disruption depends on its direct disruptive action on the organelle. In agreement with these findings, allicin was found to inhibit ergosterol transport from the plasma membrane to the cytoplasm, which is considered to be a cellular protective response to AmB-induced vacuole disruption in S. cerevisiae. Our study suggests that AmB lethality against C. albicans depends at least in part on its vacuole disruptive activity under the physiological condition permissive for invasive growth of the fungus.
authors.affiliation: Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.
authors.forename: Hasibagan
authors.initials: H
authors.lastname: Borjihan
authors.forename: Akira
authors.initials: A
authors.lastname: Ogita
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Fujita
authors.forename: Eiji
authors.initials: E
authors.lastname: Hirasawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects*; D015169:Colony Count, Microbial; D004220:Disulfides; D004357:Drug Synergism; D004875:Ergosterol / Q000037:antagonists & inhibitors / Q000378:metabolism; D005737:Garlic / Q000737:chemistry; D050296:Microbial Viability; D015394:Molecular Structure; D013441:Sulfinic Acids / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin; D000666:Amphotericin B; D004875:Ergosterol