The role of glucocorticoid in SIRP alpha and SHP-1 gene expression in AIHA patients.
The aim of this work was to evaluate the regulation of SIRP alpha, an inhibitory phagocyte receptor, and the phosphatase SHP-1 in monocytes of patients with autoimmune hemolytic anemia, and the role of dexamethasone on SIRP alpha and SHP-1 gene expression and erythrophagocytosis in vitro. SIRP alpha and SHP-1 expression was higher in monocytes from AIHA patients compared with normal, returning to normal after glucocorticoid therapy. SIRP alpha and SHP-1 mRNA expression was upregulated in healthy monocytes treated with dexamethasone compared with basal; however, the erythrophagocytic ability was not altered. Our results point to a minor role of SIRP alpha and SHP-1 in determining AIHA.
de Almeida
Ana Carolina
AC
Center for Investigation in Pediatrics and Department of Pharmacology, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
Barbosa
Soraya Massaro
SM
Barjas-Castro
Maria de Lourdes
Mde L
Olalla-Saad
Sara Teresinha
ST
Condino-Neto
Antonio
A
D000744:Anemia, Hemolytic, Autoimmune / Q000235:genetics / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000276:immunology*; D005938:Glucocorticoids / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D000943:Antigens, Differentiation; D005938:Glucocorticoids; D011971:Receptors, Immunologic; C501920:SIRPA protein, human; D003907:Dexamethasone; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6