title: Oxidative damage in trout erythrocyte in response to "in vitro" copper exposure.
abstract: The oxidative action of copper on different trout Oncorhynchus mykiss erythrocyte components was studied. The results indicate that: --cupric ions differently influence the oxidative status of two trout hemoglobin components I and IV (HbI and HbIV) having different structural and functional properties; --reactive oxygen species (ROS) production associated with hemoglobins autoxidation is not influenced by the presence of copper sulfate; --the susceptibility to hemolysis increases in the presence of copper only when the erythrocyte suspension is incubated in air; the effect of copper is almost absent for carbon monoxide-saturated erythrocyte suspensions; --DNA damage due to copper was not observed in our experimental conditions. The data obtained are important for the analysis of the environmental risks produced by copper on fish.
authors.affiliation: Department of Molecular Cellular and Animal Biology, University of Camerino, Via Gentile III da Varano, 62032 Camerino MC, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Fedeli
authors.forename: Manuel
authors.initials: M
authors.lastname: Carloni
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Falcioni
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D007558:Italy; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D014337:Trout / Q000097:blood* / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D017382:Reactive Oxygen Species; D003300:Copper