title: Reliability of lightguide spectrophotometry (O2C) for the investigation of skin tissue microvascular blood flow and tissue oxygen supply in diabetic and nondiabetic subjects.
abstract: OBJECTIVE
  Skin microvascular assessment has progressed to an important evaluation in patients with diabetes mellitus. This study was done to evaluate a new device using micro-lightguide spectrophotometry in the assessment of skin microvascular function.
  
  
  METHODS
  Twenty nondiabetic subjects (age 46.6 +/- 14.8 years; mean +/- SD) and 20 diabetic patients (age 59.4 +/- 8.4 years) participated in repeated microvascular measurements using micro-lightguide spectrophotometry. This technique allows simultaneous, noninvasive measurement of microvascular blood flow and hemoglobin oxygenation (SO(2)) at the same anatomical area in different tissue layers. A skin probe was placed on nonhairy skin at the thenar eminence of the left hand for the measurement of SO(2), and the postischemic reactive hyperemia response (PRH) was measured in skin and underlying muscle tissue.
  
  
  RESULTS
  Repeated measurements in PRH revealed a good correlation at the superficial skin layer (r = 0.97, p < 0.0001) with a coefficient of variation at 9.2 +/- 1.7% and at the superficial muscle layer (r = 0.80, p < 0.0002) with a coefficient of variation at 9.7 +/- 1.5%. A slightly weaker correlation was observed for the SO(2) measurement at the skin layer (r = 0.69 +/- p < 0.0001) with a coefficient of variation at 17.5 +/- 3.8% and at the muscle layer (r = 0.48; p = 0.0016) with a coefficient of variation at 18.1 +/- 10.5%.
  
  
  CONCLUSIONS
  Lightguide spectrophotometry is an easy, noninvasive, and reliable method for simultaneous measurement of superficial microvascular blood flow by laser Doppler fluxmetry and skin oxygenation by spectrophotometry. Further studies are required to clarify the validity of these measures in special patient populations such as diabetes mellitus with specified microvascular complications.
authors.affiliation: Institute for Clinical Research and Development, Mainz, Germany. thomasf@ikfe.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Forst
authors.forename: Cloth
authors.initials: C
authors.lastname: Hohberg
authors.forename: Eda
authors.initials: E
authors.lastname: Tarakci
authors.forename: Senait
authors.initials: S
authors.lastname: Forst
authors.forename: Peter
authors.initials: P
authors.lastname: Kann
authors.forename: Andreas
authors.initials: A
authors.lastname: Pfützner