title: [Properties of hybridization isotherms upon binding of ligands on microchips].
abstract: A simple thermodynamic model describing the microarray oligo-target hybridization has been constructed. The relationship between the hybridization signal intensity and Gibbs free energy for oligo-target duplex formation has been considered. The behavior of this function, which we called energetical hybridization isotherm, in response to target concentration change was modeled at different ratios of oligo-probes/target concentrations. The results of modeling were compared with the relevant and currently available data from microarray adsorption experiments.
authors.forename: M V
authors.initials: MV
authors.lastname: Golovkin
authors.forename: O V
authors.initials: OV
authors.lastname: Matveeva
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Nechipurenko
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D013816:Thermodynamics
chemical_list: D015342:DNA Probes