The effect of titanium particles on rat bone marrow stem cells in vitro.
In arthroplasty prostheses and dental implant, titanium is an excellent biocompatible material for its advanced physical qualities and better biocompatibility. However, it was reported that high ratios of titanium particles can be liberated due to the continual loading or articulation cycles of the implant. Because bone marrow stem cells (BMSCs) located adjacent to the implant are critical contributors to osseous tissue integrity, this study researched the influence of titanium particles on BMSCs' viability, proliferation, and cell skeleton. In addition, the phagocytosis of titanium particles by BMSCs and expression of tumor suppressor protein p53 were also examined. It was found that exposure of BMSCs to titanium particles disrupted their viability and proliferation in vitro, which may due to the phagocytosis of titanium particles by BMSCs. Moreover, cell skeleton was destroyed and the p53 protein level increased as the titanium particles were added. For these results, it was concluded that titanium particles had a cytotoxic effect on BMSCs in vitro and would inhibit the bone formation around the implant.
Meng
Bo
B
State Key Laboratory of Oral Diseases, Westchina College of Stomatology, Sichuan University, No14, 3rd Section, South Renmin Road, Chengdu 610041, PR China.
Chen
Junjie
J
Guo
Dawei
D
Ye
Qingsong
Q
Liang
Xing
X
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014025:Titanium / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D001672:Biocompatible Materials; D016159:Tumor Suppressor Protein p53; D014025:Titanium