Flavobacterium glycines sp. nov., a facultative methylotroph isolated from the rhizosphere of soybean.
An aerobic, yellow-pigmented, facultatively methylotrophic, Gram-staining-negative, non-spore-forming bacterium, designated strain Gm-149(T), was isolated from the rhizosphere of cultivated soybean in India. Cells were motile by gliding. The predominant cellular fatty acids were iso-C(15 : 0), summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1) ω 7c), C(16 : 0) 3-OH and anteiso-C(15 : 0), and the major isoprenoid quinone was MK-6. The G+C content of the genomic DNA of strain Gm-149(T) was 35.6 mol%. Comparative 16S rRNA gene sequence analysis showed that strain Gm-149(T) formed a distinct phyletic line within the genus Flavobacterium. Based on levels of pairwise 16S rRNA gene sequence similarity, strain Gm-149(T) was related most closely to the type strain of Flavobacterium daejeonense (97.1 %), but the level of DNA-DNA relatedness between these two strains was about 11.2 %. On the basis of phenotypic and genotypic data, strain Gm-149(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium glycines sp. nov. is proposed. The type strain is Gm-149(T) (=ICMP 17618(T)=NBRC 105008(T)).
Madhaiyan
Munusamy
M
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604.|Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Poonguzhali
Selvaraj
S
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Lee
Jung-Sook
JS
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon 305-806, Republic of Korea.
Lee
Keun Chul
KC
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon 305-806, Republic of Korea.
Sundaram
Subbiah
S
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
D052818:Autotrophic Processes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D000432:Methanol