title: Adipocyte culture medium stimulates invasiveness of MDA-MB-231 cell via CCL20 production.
abstract: We explored whether adipocyte culture medium affects the secreted chemokine profile of tumor cells, because adipocytes stimulate progression or metastasis of breast cancer cells, and chemokines secreted from tumor cells are involved in these processes. CCL20 expression was dramatically increased, and an NF-kappaB blocker completely inhibited adipocyte culture medium-induced CCL20 expression in MDA-MB-231 cells. We showed that adipocyte culture medium increased the production of TNF-alpha in MDA-MB-231 cells, which stimulated CCL20 expression in an autocrine fashion. Our data also showed that CCL20 increased the migration and invasiveness of MDA-MB-231 cells, but did not affect the proliferation of these cells.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, Korea.
authors.forename: Kun-Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Ahmi
authors.initials: A
authors.lastname: Baek
authors.forename: Yun S
authors.initials: YS
authors.lastname: Park
authors.forename: Mi Y
authors.initials: MY
authors.lastname: Park
authors.forename: Jae H
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Lim
authors.forename: Myeong-Sok
authors.initials: MS
authors.lastname: Lee
authors.forename: Suk R
authors.initials: SR
authors.lastname: Yoon
authors.forename: Hee G
authors.initials: HG
authors.lastname: Lee
authors.forename: Yoosik
authors.initials: Y
authors.lastname: Yoon
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Young
authors.initials: Y
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054418:Chemokine CCL20 / Q000096:biosynthesis*; D018925:Chemokines / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D018450:Disease Progression; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C108355:CCL20 protein, human; D054418:Chemokine CCL20; D018925:Chemokines; D003470:Culture Media; D014409:Tumor Necrosis Factor-alpha