[Study on the constituents of petroleum ether fraction of Buxus microphylla].
OBJECTIVE
To study the chemical constituents from the petroleum ether fraction of Buxus microphylla.


METHODS
The petroleum ether fraction of Buxus microphylla was isolated and identified by silica gel column chromatography. And the anticancer activity of different chemical constituents was measured by MTT reduction test.


RESULTS
Eight compounds were isolated and identified as lupeol (1), butulin (3), beta-sitosterol (4), stigmasterol (5), dibutyl phthalate (6), 3beta, 30-dihydroxy-lup-20 (29) ene (7), daucosterol (8). Compound 7 inhibited KB cells' proliferation in a dose-dependent manner.


CONCLUSIONS
Compounds 2 - 5, 7, 8 are isolated from this genus for the first time. Compound 7 has certainly anticancer effects.
Dai
Zhi-Kai
ZK
Guilin Medical College, Guilin 541004, China. dzhk110@126.com
Liang
Jun
J
Su
Xiao-Jian
XJ
Xu
Qing
Q
Zhang
Hui-Qin
HQ
D031235:Buxus / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000302:isolation & purification; D004987:Ethers; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification*; D013265:Stigmasterol / Q000737:chemistry / Q000302:isolation & purification*; D000094042:Lupanes
D003993:Dibutyl Phthalate; D004987:Ethers; D053978:Pentacyclic Triterpenes; D012855:Sitosterols; D013265:Stigmasterol; C025473:gamma-sitosterol; C010480:lupeol; D000094042:Lupanes; C011015:lyoniside