title: Application of high-resolution 1H MAS NMR spectroscopy to the analysis of intact bones from mice exposed to gamma radiation.
abstract: Herein we demonstrate that high-resolution magic angle spinning (MAS) 1H NMR can be used to profile the pathology of bone marrow rapidly and with minimal sample preparation. The spectral resolution obtained allows several metabolites to be analyzed quantitatively. The level of NMR-detectable metabolites in the epiphysis + metaphysis sections of mouse femur were significantly higher than that observed in the diaphysis of the same femur. The major metabolite damage to bone marrow resulting from either 3.0 Gy or 7.8 Gy of whole-body gamma radiation 4 days after exposure were (1) decreased total choline content, (2) increased fatty acids in bone marrow, and (3) decreased creatine content. These results suggest that the membrane choline phospholipid metabolism (MCPM) pathway and the fatty acid biosynthesis pathway were altered as a result of radiation exposure. We also found that the metabolic damage induced by radiation in the epiphysis + metaphysis sections of mouse femur was higher than that of the diaphysis of the same femur. Traditional histopathology analysis was also carried out to correlate radiation damage with changes in metabolites. Importantly, the molecular information gleaned from high-resolution MAS 1H NMR complements the pathology data.
authors.affiliation: Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jian Zhi
authors.initials: JZ
authors.lastname: Hu
authors.forename: Donald N
authors.initials: DN
authors.lastname: Rommereim
authors.forename: Mark K
authors.initials: MK
authors.lastname: Murphy
authors.forename: Richard P
authors.initials: RP
authors.lastname: Phipps
authors.forename: David L
authors.initials: DL
authors.lastname: Huso
authors.forename: John F
authors.initials: JF
authors.lastname: Dicello
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D001842:Bone and Bones / Q000737:chemistry / Q000528:radiation effects*; D002794:Choline / Q000032:analysis; D003404:Creatinine / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005720:Gamma Rays; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D014916:Whole-Body Irradiation
chemical_list: D005227:Fatty Acids; D003404:Creatinine; D002794:Choline