title: Structure elucidation and antioxidant activity of a novel alpha-(1-->3),(1-->4)-D-glucan from Aconitum kusnezoffii Reichb.
abstract: Aconitum kusnezoffii Reichb. has been used as traditional Chinese medicine over the last 2500 years, but its polysaccharides have been paid little attention by now. In the present study, a hot alkali extracted polysaccharide (AKP) from A. kusnezoffii Reichb. was characterized to be an alpha-(1-->3),(1-->4)-D-glucan with Mw 1.4 x 10(5) Da, of which (1-->3)-linked and (1-->4)-linked alpha-Glcp residues were in a ratio of 1:7. In vitro antioxidant testing indicated that AKP had significant ferrous ion-chelating ability, reducing power and scavenging effects on DPPH radical, hydroxyl radical, superoxide anion, H2O2 and self-oxidation of 1,2,3-phentriol, suggesting that it should be explored as a novel natural antioxidant.
authors.affiliation: School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin Province, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Gao
authors.forename: Hongtao
authors.initials: H
authors.lastname: Bi
authors.forename: Shuai
authors.initials: S
authors.lastname: Ma
authors.forename: Jingmei
authors.initials: J
authors.lastname: Lu
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007477:Ions; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000378:metabolism
chemical_list: C119131:(1-3)-(1-4)-glucan; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005936:Glucans; D007477:Ions; D007502:Iron Chelating Agents; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron