[Identification of metastasis-related microRNAs of hepatocellular carcinoma in hepatocellular carcinoma cell lines by quantitative real time PCR].
OBJECTIVE
To identify the metastasis-related miRNAs in hepatocellular carcinoma (HCC) cell lines.


METHODS
A qRT-PCR method was established and optimized.


RESULTS
All candidate metastasis associated miRNAs except miR-124a were expressed in high metastasis cell line MHCC97H and low metastasis cell line MHCC97L, while some miRNAs were differentially expressed between liver cancer cell line (HepG2) and hepatic cell line (L02) (P less than 0.05), these miRNAs include: miR-148b (1.96+/-0.51 vs 3.76+/-0.28), miR-9 (-4.38+/-0.86 vs -1.10+/-0.53), miR-30c (8.41+/-0.40 vs 6.82+/-0.29), miR-338 (3.14+/-0.29 vs -2.36+/-0.32), miR-34a (0.71+/-0.40 vs -2.95+/-0.26), Let-7g (-4.07+/-0.55 vs -6.98+/-0.56). miR-148b expression was about 4 times higher than miR-148a [5.46 (IQR 4.25-6.67) vs 1.29 (IQR 0.94-1.64)] in all cell line tested (Z=-5.097, P=3x10(-7)).


CONCLUSIONS
This study may help to understand the biological significance of miRNAs in HCC metastasis.
Zhao
Yue
Y
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Jia
Hu-liang
HL
Zhou
Hai-jun
HJ
Dong
Qiong-zhu
QZ
Fu
Li-yun
LY
Yan
Zhao-wei
ZW
Sun
Jian
J
Ren
Ning
N
Ye
Qing-hai
QH
Qin
Lun-xiu
LX
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D035683:MicroRNAs