title: The importance of -460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer.
abstract: OBJECTIVE
  The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) -460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue.
  
  
  METHODS
  Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT-PCR and protein analysis by ELISA.
  
  
  RESULTS
  The T-allele in the -460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either.
  
  
  CONCLUSIONS
  The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.
authors.affiliation: Vejle Hospital, Denmark. torben.hansen@slb.regionsyddanmark.dk
authors.forename: Torben F
authors.initials: TF
authors.lastname: Hansen
authors.forename: Karen-Lise G
authors.initials: KL
authors.lastname: Spindler
authors.forename: Karen A
authors.initials: KA
authors.lastname: Lorentzen
authors.forename: Dorte A
authors.initials: DA
authors.lastname: Olsen
authors.forename: Rikke F
authors.initials: RF
authors.lastname: Andersen
authors.forename: Jan
authors.initials: J
authors.lastname: Lindebjerg
authors.forename: Ivan
authors.initials: I
authors.lastname: Brandslund
authors.forename: Anders
authors.initials: A
authors.lastname: Jakobsen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000032:analysis; D012007:Rectum / Q000378:metabolism; D014018:Tissue Distribution; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A