A surface acoustic wave-based immunosensing device using a nanocrystalline ZnO film on Si.
The novelty of this study resides in the fabrication of a bio-sensing device, based on the surface acoustic wave (SAW) on a nanocrystalline ZnO film. The ZnO film was deposited using an rf magnetron sputtering at room temperature on silicon. The deposited films showed the c-axis-oriented crystallite with grain size of approximately 40 nm. The immunosensing device was fabricated using photolithographic protocols on the film. As a model biomolecular recognition and immunosensing, biospecific interaction between a 6-(2,4-dinitrophenyl)aminohexanoic acid (DNP) antigen and its antibody was employed, demonstrating the shifts of resonant frequencies on SAW immunosensing device. The device exhibited a linearity as a function of the antibody concentration in the range of 20-20,000 ng/ml.
Lee
Dae-Sik
DS
IT Convergence Components Lab., Electronics and Telecommunications Research Institute, Daejeon 305350, Korea.
Lee
Jun Hwang
JH
Luo
Jikui
J
Fu
Yongqing
Y
Milne
William I
WI
Maeng
Sunglyul
S
Jung
Mun Yeon
MY
Park
Sun Hee
SH
Yoon
Hyun C
HC
D000162:Acoustics / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008567:Membranes, Artificial; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
D008567:Membranes, Artificial; D015034:Zinc Oxide; D012825:Silicon