title: Fusarium proliferatum soft tissue infection at the site of a puncture by a plant: recovery, isolation, and direct molecular identification.
abstract: After allogeneic stem cell transplantation, a 49-year-old man developed fever and inflammation at the site of a plant puncture on a finger. A hyalohyphomycete was recovered by incubating the plant spine fragment following surgery. Amplification of the internal transcribed spacer region and 5.8S rRNA, beta-tubulin, and translation elongation factor coding genes identified Fusarium proliferatum, which was confirmed later by culture.
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. tpalmore@mail.nih.gov
authors.forename: Tara N
authors.initials: TN
authors.lastname: Palmore
authors.forename: Yvonne R
authors.initials: YR
authors.lastname: Shea
authors.forename: Richard W
authors.initials: RW
authors.lastname: Childs
authors.forename: Richard M
authors.initials: RM
authors.lastname: Sherry
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D039642:Eukaryotic Initiation Factors / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005670:Fusarium / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D010944:Plants; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000293:injuries*; D018461:Soft Tissue Infections / Q000382:microbiology*; D014404:Tubulin / Q000235:genetics; D014950:Wounds, Penetrating / Q000150:complications*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D039642:Eukaryotic Initiation Factors; D005656:Fungal Proteins; D012340:RNA, Ribosomal, 5.8S; D014404:Tubulin