Lignin-derived lignophenols attenuate oxidative and inflammatory damage to the kidney in streptozotocin-induced diabetic rats.
This study investigated the effects of lignin-derived lignophenols (LPs) on the oxidative stress and infiltration of macrophages in the kidney of streptozotocin (STZ)-induced diabetic rats. The diabetic rats were divided into four groups with 0%, 0.11%, 0.33% and 1.0% LP diets. The vehicle-injected controls were given a commercial diet. At 5 weeks, superoxide (O(2)(-)) production, macrophage kinetics, the degree of fibrosis in glomeruli and mRNA expression for monocyte chemoattractant protein-1 (MCP-1) were examined. The NADPH-stimulated O(2)(-) levels in the kidney of the diabetic rats treated with 1.0% LP were significantly lower than those in untreated diabetic rats. The number of macrophages, levels of MCP-1 mRNA expression and degree of glomerular fibrosis increased in untreated LP and these levels were significantly lower in 1.0%LP-treated rats. The results suggested that LPs suppress the excess oxidative stress, the infiltration and activation of macrophages and the glomerular expansion in STZ-induced diabetic kidneys.
Sato
Shin
S
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan. s_sato3@auhw.ac.jp
Mukai
Yuuka
Y
Yamate
Jyoji
J
Norikura
Toshio
T
Morinaga
Yae
Y
Mikame
Keigo
K
Funaoka
Masamitsu
M
Fujita
Shuzo
S
D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D007674:Kidney Diseases / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D008031:Lignin / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D010636:Phenols; D013311:Streptozocin; D008031:Lignin