title: Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.
abstract: Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit(+) CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit(+) CSCs.
authors.affiliation: Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Barile
authors.forename: Francesco
authors.initials: F
authors.lastname: Cerisoli
authors.forename: Giacomo
authors.initials: G
authors.lastname: Frati
authors.forename: Roberto
authors.initials: R
authors.lastname: Gaetani
authors.forename: Isotta
authors.initials: I
authors.lastname: Chimenti
authors.forename: Elvira
authors.initials: E
authors.lastname: Forte
authors.forename: Letizia
authors.initials: L
authors.lastname: Cassinelli
authors.forename: Laura
authors.initials: L
authors.lastname: Spinardi
authors.forename: Claudia
authors.initials: C
authors.lastname: Altomare
authors.forename: Eddy
authors.initials: E
authors.lastname: Kizana
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giacomello
authors.forename: Elisa
authors.initials: E
authors.lastname: Messina
authors.forename: Sergio
authors.initials: S
authors.lastname: Ottolenghi
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Magli
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D005260:Female; D006331:Heart Diseases / Q000473:pathology / Q000601:surgery*; D051379:Mice; D008822:Mice, Transgenic; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000637:transplantation*; D012333:RNA, Messenger / Q000235:genetics; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*
chemical_list: D012333:RNA, Messenger