Elimination of false-positive results in a luminex assay for pneumococcal antibodies.
In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.
Pickering
Jerry W
JW
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA. jerry.pickering@aruplab.com
Larson
Matthew T
MT
Martins
Thomas B
TB
Copple
Susan S
SS
Hill
Harry R
HR
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002417:Cattle; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000907:Antibodies, Bacterial; D012710:Serum Albumin, Bovine