title: Differentiation of human adipose-derived stem cells induced  by recombinantly expressed fibroblast growth factor  10 in vitro and in vivo.
abstract: The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo coculture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology.
authors.affiliation: Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, Shanghai 200062, China. zxymeng@hotmail.com
authors.forename: Xunyi
authors.initials: X
authors.lastname: Zhang
authors.forename: Minjuan
authors.initials: M
authors.lastname: Wu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiangfan
authors.initials: J
authors.lastname: Shen
authors.forename: Houqi
authors.initials: H
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000494:pharmacology*; D006801:Humans; D007267:Injections; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D051526:Fibroblast Growth Factor 10; D011994:Recombinant Proteins; D014157:Transcription Factors