Which model based on fluorescence quenching is suitable to study the interaction between trans-resveratrol and BSA?
There are several models by means of quenching fluorescence of BSA to determine the binding parameters. The binding parameters obtained from different models are quite different from each other. Which model is suitable to study the interaction between trans-resveratrol and BSA? Herein, twelve models based fluorescence quenching of BSA were compared. The number of binding sites increasing with increased binding constant for similar compounds binding to BSA maybe one approach to resolve this question. For example, here eleven flavonoids were tested to illustrate that the double logarithm regression curve is suitable to study binding polyphenols to BSA.
Wei
Xin Lin
XL
Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, Shanghai, PR China.
Xiao
Jian Bo
JB
Wang
Yuanfeng
Y
Bai
Yalong
Y
D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D005453:Fluorescence; D008433:Mathematics; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D000077185:Resveratrol; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000737:chemistry*
D000975:Antioxidants; D013267:Stilbenes; D012710:Serum Albumin, Bovine; D000077185:Resveratrol