Selective expression of the chemokine receptor XCR1 on cross-presenting dendritic cells determines cooperation with CD8+ T cells.
The expression of the chemokine receptor XCR1 and the function of its ligand XCL1 (otherwise referred to as ATAC, lymphotactin, or SCM-1) remained elusive to date. In the present report we demonstrated that XCR1 is exclusively expressed on murine CD8(+) dendritic cells (DCs) and showed that XCL1 is a potent and highly specific chemoattractant for this DC subset. CD8(+) T cells abundantly secreted XCL1 8-36 hr after antigen recognition on CD8(+) DCs in vivo, in a period in which stable T cell-DC interactions are known to occur. Functionally, XCL1 increased the pool of antigen-specific CD8(+) T cells and their capacity to secrete IFN-gamma. Absence of XCL1 impaired the development of cytotoxicity to antigens cross-presented by CD8(+) DCs. The XCL1-XCR1 axis thus emerges as an integral component in the development of efficient cytotoxic immunity in vivo.
Dorner
Brigitte G
BG
Molecular Immunology, Robert Koch-Institute, 13353 Berlin, Germany.
Dorner
Martin B
MB
Zhou
Xuefei
X
Opitz
Corinna
C
Mora
Ahmed
A
Güttler
Steffen
S
Hutloff
Andreas
A
Mages
Hans W
HW
Ranke
Katja
K
Schaefer
Michael
M
Jack
Robert S
RS
Henn
Volker
V
Kroczek
Richard A
RA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D019744:Chemokines, C / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology
D019744:Chemokines, C; D043562:Receptors, G-Protein-Coupled; C091671:XCR1 protein, human; C486249:Xcl1 protein, mouse