title: Phenotypical and functional characterization of T helper 17 cells in multiple sclerosis.
abstract: Multiple sclerosis is a T cell-mediated demyelinating disease of the central nervous system. Interleukin-17-producing T helper cells, named Th17 cells, represent a novel CD4+ T cell effector subset involved in the response against extracellular pathogens. In addition, Th17 cells are pathogenic in several animal models of autoimmune disease, including the animal model for multiple sclerosis, but their function in multiple sclerosis remains to be elucidated. In this study, we analysed the frequency and the phenotype of Th17 cells in the cerebrospinal fluid and peripheral blood of multiple sclerosis patients. We show that the frequency of Th17 cells is significantly higher in the cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis during relapse, in comparison to relapsing-remitting patients in remission or to patients with other non-inflammatory neurological diseases. Similarly, in patients with clinically isolated syndrome during their first neurological episode, Th17 cells are more abundant than in clinically isolated syndrome patients with no acute symptoms. Patients with inflammatory neurological diseases other than multiple sclerosis also showed increased frequency of Th17 cells compared to patients with no inflammatory diseases. To assess a potential pathological impact of Th17 cells in disease, we generated T cell clones from the cerebrospinal fluid and peripheral blood of patients with multiple sclerosis. We found that Th17 clones expressed higher basal levels of the activation markers CD5, CD69, CD2 and human leukocyte antigen-DR as well as of the CD28-related family of co-stimulatory molecules, when compared to Th1 clones, and confirmed these findings with ex vivo human T cells. Molecules involved in T cell adhesion to endothelium, such as CD49d, CD6 and the melanoma cell adhesion molecule, were also more abundant on the Th17 than on the Th1 cells. Furthermore, functional assays showed that Th17 clones were more prone than Th1 clones to melanoma cell adhesion molecule-mediated adhesion to endothelial cells, and that Th17 cells had a higher proliferative capacity and were less susceptible to suppression than Th1 cells. Altogether our data suggest that Th17 cells display a high pathogenic potential and may constitute a relevant pathogenic subset in multiple sclerosis.
authors.affiliation: Institute for Neuroimmunology and Clinical Multiple Sclerosis Research, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Brucklacher-Waldert
authors.forename: Klarissa
authors.initials: K
authors.lastname: Stuerner
authors.forename: Manuela
authors.initials: M
authors.lastname: Kolster
authors.forename: Julia
authors.initials: J
authors.lastname: Wolthausen
authors.forename: Eva
authors.initials: E
authors.lastname: Tolosa
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000032:analysis / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D051929:CD146 Antigen / Q000032:analysis / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000166:cytology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology* / Q000503:physiopathology; D010641:Phenotype; D012008:Recurrence; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology* / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D051929:CD146 Antigen; D015815:Cell Adhesion Molecules; C497902:MCAM protein, human