title: Pyrosequencing the Manduca sexta larval midgut transcriptome: messages for digestion, detoxification and defence.
abstract: The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome. We generated over 387,000 midgut ESTs, using a combination of Sanger and 454 sequencing, and classified predicted proteins into those involved in digestion, detoxification and immunity. In many cases the depth of 454 pyrosequencing coverage allowed us to define the entire cDNA sequence of a particular gene. Many new M. sexta genes are described including up to 36 new cytochrome P450s, some of which have been implicated in the metabolism of host plant-derived nicotine. New lepidopteran gene families such as the beta-fructofuranosidases, previously thought to be restricted to Bombyx mori, are also described. An unexpectedly high number of ESTs were involved in immunity, for example 39 contigs encoding serpins, and the increasingly appreciated role of the midgut in insect immunity is discussed. Similar studies of other tissues will allow for a tissue by tissue description of the M. sexta transcriptome and will form an essential complimentary step on the road to genome sequencing and annotation.
authors.affiliation: School of Biosciences, University of Exeter, Cornwall campus, Penryn, UK. y.pauchet@exeter.ac.uk
authors.forename: Y
authors.initials: Y
authors.lastname: Pauchet
authors.forename: P
authors.initials: P
authors.lastname: Wilkinson
authors.forename: H
authors.initials: H
authors.lastname: Vogel
authors.forename: D R
authors.initials: DR
authors.lastname: Nelson
authors.forename: S E
authors.initials: SE
authors.lastname: Reynolds
authors.forename: D G
authors.initials: DG
authors.lastname: Heckel
authors.forename: R H
authors.initials: RH
authors.lastname: ffrench-Constant
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004063:Digestion; D020224:Expressed Sequence Tags; D041981:Gastrointestinal Tract / Q000378:metabolism; D020869:Gene Expression Profiling; D022761:Gene Transfer, Horizontal; D008658:Inactivation, Metabolic; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D003577:Cytochrome P-450 Enzyme System; D043324:beta-Fructofuranosidase