title: Test characteristics of commercial influenza assays for detecting pandemic influenza A (H1N1) in children.
abstract: We assessed the test characteristics of 2 influenza antigen tests, a rapid immunoassay and a direct fluorescence antibody (DFA) assay, in detecting pandemic influenza A (H1N1) in children up to 18 years of age, using polymerase chain reaction as the standard. The sensitivities of BinaxNOW (59.6%) and direct fluorescence antibody (57.3%) were similar. The specificity of each test was >99%.
authors.affiliation: Division of Infectious Diseases, Department of Medicine , Children's Hospital Boston, Boston, MA 02115, USA. thomas.sandora@childrens.harvard.edu
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Sandora
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Smole
authors.forename: Grace M
authors.initials: GM
authors.lastname: Lee
authors.forename: Sarita
authors.initials: S
authors.lastname: Chung
authors.forename: Laura
authors.initials: L
authors.lastname: Williams
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: McAdam
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D008297:Male; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic