A selenide-based approach to photochemical cleavage of peptide and protein backbones at engineered backbone esters.
A strategy for photochemical cleavage of peptide and protein backbones is described, which is based on a selenide-mediated cleavage of a backbone ester moiety. Studies in model systems establish the viability of the chemistry and suggest the method could be a valuable tool for chemical biology studies of proteins.
Eastwood
Amy L
AL
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Blum
Angela P
AP
Zacharias
Niki M
NM
Dougherty
Dennis A
DA
D004952:Esters; D006868:Hydrolysis; D008722:Methods; D010455:Peptides / Q000737:chemistry*; D010782:Photolysis; D011506:Proteins / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*
D004952:Esters; D010455:Peptides; D011506:Proteins; D018036:Selenium Compounds