Novel human papillomavirus type 18 replicon and its application in screening the antiviral effects of cytokines.
Human papillomaviruses (HPVs) infect the stratified epithelial organ. The infection induces benign tumors, which occasionally progress into malignant tumors. To elucidate the virus-induced tumorigenesis, an understanding of the lifecycle of HPV is crucial. In this report, we developed a new system for the analysis of the HPV lifecycle. The new system consists of a novel HPV replicon and an organotypic "raft" culture, by which the HPV-DNA is maintained stably in normal human keratinocytes for a long period and the viral vegetative replication is reproduced. This system will benefit biochemical and genetic studies on the lifecycle of HPV and tumorigenesis. This system is also valuable in screening for antiviral compounds. We confirmed its usefulness by evaluating the antivirus effect of cytokines.
Satsuka
Ayano
A
Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Yoshida
Satoshi
S
Kajitani
Naoko
N
Nakamura
Hiroyasu
H
Sakai
Hiroyuki
H
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis; D052161:Human papillomavirus 18 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D012093:Replicon / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D016207:Cytokines; D004279:DNA, Viral