title: Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis.
abstract: OBJECTIVE
  Researchers have launched a new area of febrile investigations on the autophagy-related gene Beclin 1. Our aim is to investigate whether Beclin 1 expression is altered in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.
  
  
  METHODS
  We collected tissue samples from the eutopic endometria of 30 women with adenomyosis and 32 healthy women undergoing surgery for benign indications. We cultured the stromal cells of the eutopic endometria. Beclin 1 expression of the cultured stromal cells and tissues was assessed by reverse transcription polymerase chain reaction and western blot analysis.
  
  
  RESULTS
  Beclin 1 messenger RNA (mRNA) expression in cultured stromal cells of eutopic endometria and endometrial tissues of women with adenomyosis was significantly lower than that of controls (P < 0.05). Beclin 1 protein expression in cultured stromal cells of eutopic endometria and endometrial tissues of adenomyosis was also significantly lower compared with that of controls (P < 0.01). Beclin 1 protein expression in eutopic endometrial tissues was negatively correlated with serum CA125 (r = -0.307, P = 0.015), and pelvic pain (r = -0.542, P = 0.000).
  
  
  CONCLUSIONS
  The study revealed Beclin 1 mRNA and protein expression were significantly decreased in eutopic endometria of women with adenomyosis. Moreover, Beclin 1 was negatively correlated with serum CA125 and pelvic pain. Beclin 1 might contribute to the pathogenesis and progression of endometriosis. Further research on autophagy of adenomyosis is required.
authors.affiliation: Department of Gynecology, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Ren
authors.forename: Lin
authors.initials: L
authors.lastname: Mu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy; D000071186:Beclin-1; D015153:Blotting, Western; D018394:CA-125 Antigen / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D010147:Pain Measurement; D017699:Pelvic Pain / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D048348:Reverse Transcription; D017421:Sequence Analysis; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger