[Optimization of the industrial production of the recombinant precursor of human insulin].
Conditions were found at the analytical level for the solubilization of a recombinant insulin precursor from inclusion bodies in different buffer systems at a wide pH range in the presence of different reducing (dithiothreitol, dithioerythritol) and chaotropic agents (urea, guanidine hydrochloride) and the subsequent renaturation with the use of redox pairs (cysteine-cystine, oxidized glutathione-reduced glutathione, and others). The scaling of the method for the production of the active substance of genetically engineered human insulin has been performed.
Gusarova
V D
VD
Gusarov
D A
DA
Mironov
A F
AF
Bairamashvili
D I
DI
Miroshnikov
A I
AI
D004345:Drug Industry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D011384:Proinsulin / Q000096:biosynthesis / Q000302:isolation & purification*; D011489:Protein Denaturation; D017510:Protein Folding; D020673:Protein Renaturation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000378:metabolism
D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; D011384:Proinsulin