title: DBTSS provides a tissue specific dynamic view of Transcription Start Sites.
abstract: DataBase of Transcription Start Sites (DBTSS) is a database which contains precise positional information for transcription start sites (TSSs) of eukaryotic mRNAs. In this update, we included 330 million new tags generated by massively sequencing the 5'-end of oligo-cap selected cDNAs in humans and mice. The tags were collected from normal fetal or adult human tissues, including brain, thymus, liver, kidney and heart, from 6 human cell lines in 21 diverse growth conditions as well as from mouse NIH3T3 cell line: altogether 31 different cell types or culture conditions are represented. This unprecedented increase in depth of data now allows DBTSS to faithfully represent the dynamically changing landscape of TSSs in different cell types and conditions, during development and in the course of evolution. Differential usage of alternative 5'-ends across cell types and conditions can be viewed in a series of new interfaces. Promoter sequence information is now displayed in a comparative genomics viewer where evolutionary turnover of the TSSs can be evaluated. DBTSS can be accessed at http://dbtss.hgc.jp/.
authors.affiliation: Frontier Research Initiative, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Riu
authors.initials: R
authors.lastname: Yamashita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakaguri
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Kenta
authors.initials: K
authors.lastname: Nakai
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology / Q000379:methods* / Q000639:trends; D030541:Databases, Genetic; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods; D020407:Internet; D051379:Mice; D041681:NIH 3T3 Cells; D024363:Transcription Initiation Site; D014584:User-Computer Interface