title: X-ray structure and biophysical properties of rabbit fibroblast growth factor 1.
abstract: The rabbit is an important and de facto animal model in the study of ischemic disease and angiogenic therapy. Additionally, fibroblast growth factor 1 (FGF-1) is emerging as one of the most important growth factors for novel proangiogenic and pro-arteriogenic therapy. However, despite its significance, the fundamental biophysical properties of rabbit FGF-1, including its X-ray structure, have never been reported. Here, the cloning, crystallization, X-ray structure and determination of the biophysical properties of rabbit FGF-1 are described. The X-ray structure shows that the amino-acid differences between human and rabbit FGF-1 are solvent-exposed and therefore potentially immunogenic, while the biophysical studies identify differences in thermostability and receptor-binding affinity that distinguish rabbit FGF-1 from human FGF-1.
authors.affiliation: Department of Biomedical Sciences, Florida State University, Tallahassee, 32306-4300, USA.
authors.forename: Jihun
authors.initials: J
authors.lastname: Lee
authors.forename: Sachiko I
authors.initials: SI
authors.lastname: Blaber
authors.forename: Andre
authors.initials: A
authors.lastname: Irsigler
authors.forename: Eric
authors.initials: E
authors.lastname: Aspinwall
authors.forename: Michael
authors.initials: M
authors.lastname: Blaber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D005260:Female; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics
chemical_list: D016220:Fibroblast Growth Factor 1