Sphingobacterium shayense sp. nov., isolated from forest soil.
A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39(T), isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30-37&emsp14;°C, at pH&emsp14;6.5-8.0 and with 0-3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39(T) revealed that it is a member of the genus Sphingobacterium. Sphingobacterium mizutaii ATCC 33299(T) was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C₁₅:₀, iso-C₁₇:₀ 3-OH and summed feature 3 (comprising C₁₆:₁ω6c and/or C₁₆:₁ω7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39(T) (=CCTCC AB 209006(T) =NRRL B-59203(T)).
He
Xiang
X
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Xiao
Ting
T
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Kuang
Haiju
H
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Lan
Xiaojun
X
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Tudahong
Maripat
M
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Osman
Ghenijan
G
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
Fang
Chengxiang
C
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Rahman
Erkin
E
College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D032107:Populus / Q000254:growth & development; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature; D014197:Trees
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride