Comparative analysis of the grain proteome fraction in barley genotypes with contrasting salinity tolerance during germination.
In the present paper, we based a search for candidates underlying different levels of salinity tolerance during germination in the Oregon Wolfe Barley mapping population (DOM x REC) by proteomic profiling of the mature grain of lines showing differing levels of salinity tolerance. By contrasting the parents DOM and REC, displaying divergent stress responses, and two tolerant and two sensitive segregants, six protein spots were identified that showed a differential abundance between the tolerant and the sensitive lines. The tolerant lines expressed a higher level of 6-phosphogluconate dehydrogenase and glucose/ribitol dehydrogenase (Glc/RibDH). Both proteins were heterologously over-expressed in an osmo-sensitive yeast strain and over-expression of Glc/RibDH resulted in an enhanced ability of yeast transformants to grow on salt containing media. A quantitative trait locus (QTL) analysis of the population germinating at different salt concentrations led to the identification of two chromosome regions on 5H and one on 7H associated with salt stress response. A dense barley transcript map was employed to map the genomic region of all identified proteins. Two of these, heat-shock protein 70 and Glc/RibDH, co-localized with the identified QTL on chromosome 5H. The putative functional role of the candidates is discussed.
Witzel
Katja
K
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany.
Weidner
Annette
A
Surabhi
Giridara-Kumar
GK
Varshney
Rajeev K
RK
Kunze
Gotthard
G
Buck-Sorlin
Gerhard H
GH
Börner
Andreas
A
Mock
Hans-Peter
HP
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D055028:Comparative Genomic Hybridization; D017343:Genes, Plant; D018525:Germination; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040641:Quantitative Trait Loci; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D020543:Proteome; D013401:Sugar Alcohol Dehydrogenases; D010734:Phosphogluconate Dehydrogenase