title: Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF.
abstract: OBJECTIVE
  To investigate whether follicular fluid lipid-soluble micronutrients are associated with embryo morphology parameters during IVF.
  
  
  METHODS
  Follicle fluid and oocytes were obtained prospectively from 81 women. Embryo morphology parameters were used as surrogate markers of oocyte health. HDL lipids and lipid-soluble micronutrients were analyzed by high-pressure liquid chromatography. Non-parametric bi-variate analysis and multivariable ordinal logistic regression models were employed to examine associations between biochemical and embryo morphology parameters.
  
  
  RESULTS
  Follicular fluid HDL cholesterol (r = -0.47, p < 0.01), alpha-tocopherol (r = -0.41, p < 0.01), delta-tocopherol (r = -0.38, p < 0.05) and beta-cryptoxanthine (r = -0.42, p < 0.01) are negatively correlated with embryo fragmentation. Ordinal logistic regression models indicate that a 0.1 mumol/L increase in beta-cryptoxanthine, adjusted for gamma-tocopherol, is associated with a 75% decrease in the cumulative odds of higher embryo fragmentation (p = 0.010).
  
  
  CONCLUSIONS
  Follicular fluid HDL micronutrients may play an important role in the development of the human oocyte as evident by embryo fragmentation during IVF.
authors.affiliation: Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, 26 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA. rwbrowne@buffalo.edu
authors.forename: Richard W
authors.initials: RW
authors.lastname: Browne
authors.forename: Michael S
authors.initials: MS
authors.lastname: Bloom
authors.forename: Wendy B
authors.initials: WB
authors.lastname: Shelly
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Ocque
authors.forename: Heather G
authors.initials: HG
authors.lastname: Huddleston
authors.forename: Victor Y
authors.initials: VY
authors.lastname: Fujimoto
mesh_terms: D000328:Adult; D008076:Cholesterol, HDL / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D016015:Logistic Models; D008297:Male; D018977:Micronutrients / Q000378:metabolism*; D015999:Multivariate Analysis; D011247:Pregnancy; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D018977:Micronutrients