Influence of beta-galactose-specific mitochondrial lectins from prostate hyperplasic tissue on mitochondrial properties.
Galactose-specific lectins (Gal-lectins) were isolated from the mitochondrial fraction of prostate post-operational hyperplasic tissue of two diagnoses: benign prostate hyperplasic tissue with low-grade intraepithelial neoplasia (LGPIN) and benign prostate hyperplasic tissue with atypical adenomatous hyperplasia (AAH). They had similar molecular weight and other properties. Effects of these lectins were investigated in vitro model experiments on bovine liver cells mitochondrial properties. Time-dependent changes: (i) in the amount of H(2)O(2); (ii) redox state of Cu in cytochrome oxidase and (iii) redox state of heme in cytochrome a+a(3) (cyt a+a(3)) of cytochrome c oxidase complex were studied. Gal-lectins from both sources increase the amount of H(2)O(2) and decrease the redox state of Cu in cytochrome oxidase and heme in cyt a+a(3). However the Gal-lectin from tissue with more severed transformation (AAH) expresses significantly more strong and long-lasting influence. These effects are mediated by galactose binding domain of the lectins as are completely abolished by the inclusion of galactose in reaction medium. Accumulation of H(2)O(2) and long-lasting decrease in the redox state of key enzymes of mitochondrial respiration chain could induce defective functioning of these organelles and whole cells. Obtained data point the possible way, which enhances further transformation of prostate tissue by release of Gal-lectins from damaged mitochondria.
Davitashvili
Elen
E
Faculty of Exact and Natural Sciences, Department of Biology, I. Javakhishvili Tbilisi State University, Georgia. 2, University Str., 0143 Tbilisi, Republic of Georgia. edavitashvili@yahoo.com
Kvitsinadze
Nino
N
Megrelishvili
Iveta
I
Karazanashvili
Guram
G
Solomonia
Revaz
R
Aleksidze
Nugzar
N
D000368:Aged; D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D037161:Galectins / Q000502:physiology*; D006418:Heme / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D011470:Prostatic Hyperplasia / Q000378:metabolism*
D037161:Galectins; D006418:Heme; D003300:Copper; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D005690:Galactose