Selenium and its species distribution in above-ground plant parts of selenium enriched buckwheat (Fagopyrum esculentum Moench).
Common buckwheat (Fagopyrum esculentum Moench) was foliarly sprayed with a water solution containing 10 mg Se(VI) L(-1) at the beginning of flowering. The total Se content in plant parts in the untreated group was low, whereas in the Se-sprayed group it was approximately 50- to 500-fold higher, depending on the plant part (708-4231 ng Se g(-1) DM(-1) (DM: dry matter)). We observed a similar distribution of Se in plant parts in both control and treated groups, with the highest difference in Se content being in ripe seeds. Water-soluble Se compounds were extracted by enzymatic hydrolysis with protease XIV, resulting in above 63% of soluble Se from seeds, approximately 14% from stems, leaves and inflorescences and less than 1% from husks. Se-species were determined in enzymatic extracts using HPLC-UV-HG-AFS (HPLC-hydride generation-atomic fluorescence spectrometry with UV treatment). The main Se species found in seeds was SeMet ( approximately 60% according to total Se content), while in stems, leaves and inflorescences the only form of soluble Se present was Se(VI) (up to 10% of total Se). In husks no Se-species were detected. We observed an instability of Se(IV) in seed extracts as a possible consequence of binding to the matrix components. Therefore, special care concerning sample extraction and the storage time of the extracts should be taken.
Vogrincic
Maja
M
Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Cuderman
Petra
P
Kreft
Ivan
I
Stibilj
Vekoslava
V
D019612:Fagopyrum / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000378:metabolism*; D012643:Selenium / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D012643:Selenium