title: 1H, 15N and 13C assignments of the dimeric C-terminal domain of HIV-1 capsid protein.
abstract: HIV-1 capsid protein (CA) encloses the viral RNA genome and forms a conical-shaped particle in the mature HIV-1 virion, with orderly capsid assembly and disassembly critically important for viral infectivity. The 231 residue CA is composed of two helical domains, connected by a short linker sequence. In solution, CA exhibits concentration dependent dimerization which is mediated by the C-terminal domain (CTD). Here, we present nearly complete (1)H, (15)N and (13)C assignments for the 20 kDa homodimeric CA-CTD, a prerequisite for structural characterization of the CA-CTD dimer.
authors.affiliation: Department of Structural Biology and Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Jung
authors.forename: In-Ja L
authors.initials: IJ
authors.lastname: Byeon
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Ahn
authors.forename: Jason
authors.initials: J
authors.lastname: Concel
authors.forename: Angela M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics; D002247:Carbon Isotopes / Q000737:chemistry; D015497:HIV-1 / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D008958:Models, Molecular; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D054301:gag Gene Products, Human Immunodeficiency Virus; D006859:Hydrogen