CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: use for quality control.
Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.
Evrard
Bertrand
B
CHU Clermont-Ferrand, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France. bevrard@chu-clermontferrand.fr
Dosgilbert
Annie
A
Jacquemot
Nathalie
N
Demeocq
François
F
Gilles
Thibault
T
Chassagne
Jacques
J
Berger
Marc
M
Tridon
Arlette
A
D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins; D005456:Fluorescent Dyes / Q000032:analysis*; D006084:Graft Rejection / Q000097:blood / Q000628:therapy; D006086:Graft vs Host Disease / Q000097:blood / Q000628:therapy; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D017893:Photopheresis / Q000379:methods* / Q000592:standards; D010835:Phytohemagglutinins / Q000494:pharmacology; D011786:Quality Control; D012494:Sampling Studies; D012751:Sezary Syndrome / Q000097:blood / Q000628:therapy; D012871:Skin Diseases / Q000097:blood / Q000628:therapy; D013388:Succinimides / Q000097:blood*; D013936:Thymidine / Q000097:blood; D014316:Tritium / Q000097:blood
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010835:Phytohemagglutinins; D013388:Succinimides; D014316:Tritium; D013936:Thymidine