[Clinicopathological significance of microRNA-21 and miR-125 expression in colorectal cancer].
OBJECTIVE
To investigate the expression of microRNA(miR)-21 and miR-125 in colorectal cancer (CRC) and its relationship with clinicopathological features.


METHODS
Quantitative real-time PCR was applied to examine the expression of miR-21 and miR-125 in 100 primary CRC specimens which were diagnosed and operated in West China Hospital between 2006 and 2007, in comparison with the corresponding normal mucosa specimens. The relationship between the expression of miRNAs and clinicopathological features was analyzed.


RESULTS
The expression of miR-21 in CRC was up-regulated by 2.3 times compared to normal mucosa (P =0.025), while the expression of miR-125 was down-regulated by 3.3 times in comparison with normal mucosa (P =0.005). Furthermore, the expression of miR-21 was related to TNM stage (P =0.028) and local invasion (P =0.023). On the other hand, no significant relationship was found between the expression of miR-125 and clinicopathological features (P >0.05).


CONCLUSIONS
The over-expression of miR-21 may play a role in the development and progression of CRC, while miR-125 may not be related to the pathogenesis of CRC.
Zhang
Yi
Y
Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Zong-guang
ZG
Wang
Ling
L
Zhang
Peng
P
Wang
Mo-jin
MJ
Cui
Chang-fu
CF
Guan
Jian-ting
JT
Chen
Ke-ling
KL
Zhan
Lan
L
D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C531888:MIRN125 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D012313:RNA