Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry.
Hepatotoxic pyrrolizidine alkaloid (HPA)-containing plants have always been a threat to human and livestock health worldwide. Adonifoline, a main HPA in Senecio scandens Buch.-Ham. ex D. Don (Qianli guang), was used officially as an infusion in cases of oral and pharyngeal infections in China. In this study in vivo metabolism of adonifoline was studied for the first time by identifying the metabolites of adonifoline present in bile, urine and feces of rats using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS(n)) (ion trap) as well as liquid chromatography/electrospray ionization high-resolution mass spectrometry (LC/ESI-HRMS) (quadrupole-time of flight). In total 19 metabolites were identified and, among them, retronecine-N-oxides were confirmed by matching their fragmentation patterns with their fully characterized synthetic compounds. These metabolites are all involved in both phase I and phase II metabolic processes and the principal in vivo metabolism pathways of adonifoline were proposed.
Xiong
Aizhen
A
The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China.
Yang
Li
L
He
Yuqi
Y
Zhang
Fang
F
Wang
Jun
J
Han
Han
H
Wang
Changhong
C
Bligh
S W Annie
SW
Wang
Zhengtao
Z
D000818:Animals; D001646:Bile / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D007783:Lactones / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D008297:Male; D011763:Pyrrolizidine Alkaloids / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012674:Senecio / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007783:Lactones; D011763:Pyrrolizidine Alkaloids; C546218:adonifoline