Nocardioides mesophilus sp. nov., isolated from soil.
A short coccoid- to rod-shaped, motile, mesophilic actinobacterium, strain MSL-22(T), was isolated from soil on Bigeum Island, Korea. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MSL-22(T) formed an evolutionary lineage within the radiation of the genus Nocardioides. In particular, it formed a monophyletic lineage with Nocardioides jensenii KCTC 9134(T) with which it shared the highest sequence similarity of about 97.3%. However, DNA-DNA relatedness demonstrated that strain MSL-22(T) was distinct from its closest phylogenetic neighbours. The cell-wall peptidoglycan of strain MSL-22(T) contained LL-diaminopimelic acid. The predominant menaquinone was MK-8(H₄). Strain MSL-22(T) had a cellular fatty acid profile containing straight-chain, branched, unsaturated and 10-methyl fatty acids, with iso-C₁₆:₀ as the major fatty acid. The DNA G+C content of the strain was 68.7 mol%. On the basis of phenotypic and phylogenetic evidence, the strain is separate from previously described members of the genus Nocardioides and represents a novel species in this genus, for which the name Nocardioides mesophilus sp. nov. is proposed. The type strain is MSL-22(T) (=DSM 19432(T)=KCTC 19310(T)).
Dastager
Syed G
SG
Korea Research Institute of Bioscience and Biotechnology 52, Oeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.|National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthpuram-695019, India.
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology 52, Oeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Pandey
Ashok
A
National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthpuram-695019, India.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology 52, Oeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid