Assay-dependent phytotoxicity of nanoparticles to plants.
The effects of five nanomaterials (multiwalled carbon nanotubes [MWCNTs], Ag, Cu, ZnO, Si) and their corresponding bulk counterparts on seed germination, root elongation, and biomass of Cucurbita pepo (zucchini) were investigated. The plants were grown in hydroponic solutions amended with nanoparticles or bulk material suspensions at 1000 mg/L. Seed germination was unaffected by any of the treatments, but Cu nanoparticles reduced emerging root length by 77% and 64% relative to unamended controls and seeds exposed to bulk Cu powder, respectively. During a 15-day hydroponic trial, the biomass of plants exposed to MWCNTs and Ag nanoparticles was reduced by 60% and 75%, respectively, as compared to control plants and corresponding bulk carbon and Ag powder solutions. Although bulk Cu powder reduced biomass by 69%, Cu nanoparticle exposure resulted in 90% reduction relative to control plants. Both Ag and Cu ion controls (1-1000 mg/L) and supernatant from centrifuged nanoparticle solutions (1000 mg/L) indicate that half the observed phytotoxicity is from the elemental nanoparticles themselves. The biomass and transpiration volume of zucchini exposed to Ag nanoparticles or bulk powder at 0-1000 mg/mL for 17 days was measured. Exposure to Ag nanoparticles at 500 and 100 mg/L resulted in 57% and 41% decreases in plant biomass and transpiration, respectively, as compared to controls or to plants exposed to bulk Ag. On average, zucchini shoots exposed to Ag nanoparticles contained 4.7 greater Ag concentration than did the plants from the corresponding bulk solutions. These findings demonstrate that standard phytotoxicity tests such as germination and root elongation may not be sensitive enough or appropriate when evaluating nanoparticle toxicity to terrestrial plant species.
Stampoulis
Dimitrios
D
Department of Biology and Environmental Science and Department of Physics, University of New Haven, 300 Boston Post Road, West Haven, Connecticut 06516, USA.
Sinha
Saion K
SK
White
Jason C
JC
D000383:Agriculture / Q000379:methods; D001681:Biological Assay / Q000379:methods*; D003300:Copper / Q000633:toxicity; D018556:Crops, Agricultural; D028464:Cucurbita / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D020387:Food Chain; D005506:Food Contamination; D018525:Germination / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity; D018517:Plant Roots / Q000187:drug effects; D012825:Silicon / Q000633:toxicity; D012834:Silver / Q000633:toxicity; D015034:Zinc Oxide / Q000633:toxicity
D037742:Nanotubes, Carbon; D012834:Silver; D003300:Copper; D015034:Zinc Oxide; D012825:Silicon