Microbial fed-batch production of 1,3-propanediol using raw glycerol with suspended and immobilized Klebsiella pneumoniae.
The production of 1,3-propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM 4799 using raw glycerol without purification obtained from a biodiesel production process. Fed-batch cultures with suspended cells revealed that 1,3-PD production was more effective when utilizing raw glycerol than pure glycerol (productivity after 47 h of fermentation, 0.84 g L(-1) 1 h(-1) versus 1.51 g L(-1) h(-1) with pure and raw glycerol,respectively). In addition, more than 80 g/L of 1,3-PD was produced using raw glycerol;this is the highest 1,3-PD concentration reported thus far for K. pneumoniae using raw glycerol. Repeated fed-batch fermentation with cell immobilization in a fixed-bed reactor was performed to enhance 1,3-PD production. Production of 1,3-PD increased with the cycle number (1.06 g L(-1) h(-1) versus 1.61 g L(-1) h(-1) at the first and fourth cycle, respectively)due to successful cell immobilization. During 46 cycles of fed-batch fermentation taking place over 1,460 h, a stable and reproducible 1,3-PD production performance was observed with both pure and raw glycerol. Based on our results, repeated fed batch with immobilized cells is an efficient fermentor configuration, and raw glycerol can be utilized to produce 1,3-PD without inhibitory effects caused by accumulated impurities.
Jun
Sun-Ae
SA
Center for Environmental Technology Research, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, South Korea.
Moon
Chuloo
C
Kang
Cheol-Hee
CH
Kong
Sean W
SW
Sang
Byoung-In
BI
Um
Youngsoon
Y
D056804:Biofuels; D019149:Bioreactors; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*
D056804:Biofuels; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol