Monolithic methacrylate packed 96-tips for high throughput bioanalysis.
In the pharmaceutical industry the growing number of samples to be analyzed requires high throughput and fully automated analytical techniques. Commonly used sample-preparation methods are solid-phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation. In this paper we will discus a new sample-preparation technique based on SPE for high throughput drug extraction developed and used by our group. This new sample-preparation method is based on monolithic methacrylate polymer as packing sorbent for 96-tip robotic device. Using this device a 96-well plate could be handled in 2-4min. The key aspect of the monolithic phase is that monolithic material can offer both good binding capacity and low back-pressure properties compared to e.g. silica phases. The present paper presents the successful application of monolithic 96-tips and LC-MS/MS by the sample preparation of busulphan, rescovitine, metoprolol, pindolol and local anaesthetics from human plasma samples and cyklophosphamid from mice blood samples.
Altun
Zeki
Z
Department of Chemistry & Biomedical Sciences, Karlstad University, SE-65188 Karlstad, Sweden.
Skoglund
Christina
C
Abdel-Rehim
Mohamed
M
D000818:Animals; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D004364:Pharmaceutical Preparations / Q000097:blood; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods*
D008689:Methacrylates; D004364:Pharmaceutical Preparations