title: MALDI-TOF mass spectrometry: a powerful tool to study the internalization of cell-penetrating peptides.
abstract: This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms.
authors.affiliation: Université Pierre et Marie Curie, 4 place Jussieu, 75005 Paris, France.
authors.forename: Soline
authors.initials: S
authors.lastname: Aubry
authors.forename: Baptiste
authors.initials: B
authors.lastname: Aussedat
authors.forename: Diane
authors.initials: D
authors.lastname: Delaroche
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Jiao
authors.forename: Gérard
authors.initials: G
authors.lastname: Bolbach
authors.forename: Solange
authors.initials: S
authors.lastname: Lavielle
authors.forename: Gérard
authors.initials: G
authors.lastname: Chassaing
authors.forename: Sandrine
authors.initials: S
authors.lastname: Sagan
authors.forename: Fabienne
authors.initials: F
authors.lastname: Burlina
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D057846:Cell-Penetrating Peptides; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D013438:Sulfhydryl Compounds