title: Identification of messenger RNA substrates for mouse T-STAR.
abstract: Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3' untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis.
authors.affiliation: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, P. R. China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Zeng
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: H Q
authors.initials: HQ
authors.lastname: Sun
authors.forename: D C
authors.initials: DC
authors.lastname: Tao
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: S Z
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Y X
authors.initials: YX
authors.lastname: Ma
mesh_terms: D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000737:chemistry* / Q000378:metabolism
chemical_list: C484252:Fabp9 protein, mouse; D050556:Fatty Acid-Binding Proteins; C486528:Khdrbs3 protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins