Insight into the dehydrogenase catalyzed redox reactions and electron discharge pattern during fermentative hydrogen production.
Dehydrogenase (DH) activity associated with bio-electrochemical behavior was analyzed for the first time to understand the redox reactions involved in fermentative hydrogen (H(2)) production process in concurrence with proton (H(+)) shuttling and electron (e(-)) discharge (ED) pattern. DH facilitates the availability of H(+) through redox reactions to make H(2). We have designed a comprehensive experimental study to evaluate the DH activity (H(+) shuttling) and ED to understand the biochemical process with the function of pH (5, 6, 7 and 8) and metabolic microenvironment [anaerobic, anoxic and aerobic (control)]. DH activity was observed to be higher during anaerobic operation suggesting the higher availability of H(+) and e(-) due to the inter-conversion of metabolites and the same was reflected in the voltammetry analysis. Higher H(2) production under anaerobic operation corroborated well with these findings. The DH activity associated with H(+) shuttling and ED was also correlated with the substrate degradation pattern.
Venkata Mohan
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Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad 500 607, India. vmohan_s@yahoo.com
Srikanth
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Lenin Babu
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Sarma
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D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004583:Electrons; D004867:Equipment Design; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D011522:Protons; D014874:Water Pollutants, Chemical / Q000737:chemistry; D018508:Water Purification / Q000379:methods
D011522:Protons; D014874:Water Pollutants, Chemical; D006859:Hydrogen; D010088:Oxidoreductases; D005947:Glucose; D010100:Oxygen