Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
Shaw
Kirsty J
KJ
Department of Chemistry, University of Hull, Cottingham Road, Hull, UK HU6 7RX.
Joyce
Domino A
DA
Docker
Peter T
PT
Dyer
Charlotte E
CE
Greenman
John
J
Greenway
Gillian M
GM
Haswell
Stephen J
SJ
D004247:DNA / Q000302:isolation & purification*; D004867:Equipment Design; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D009061:Mouth Mucosa / Q000166:cytology; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry; D013048:Specimen Handling / Q000191:economics / Q000295:instrumentation
D012822:Silicon Dioxide; D004247:DNA