Apoptosis and proliferation in the trigeminal placode.
The neurogenic trigeminal placode develops from the crescent-shaped panplacodal primordium which delineates the neural plate anteriorly. We show that, in Tupaia belangeri, the trigeminal placode is represented by a field of focal ectodermal thickenings which over time changes positions from as far rostral as the level of the forebrain to as far caudal as opposite rhombomere 3. Delamination proceeds rostrocaudally from the ectoderm adjacent to the rostral midbrain, and contributes neurons to the trigeminal ganglion as well as to the ciliary ganglion/oculomotor complex. Proliferative events are centered on the field prior to the peak of delamination. They are preceded, paralleled and, finally, outnumbered by apoptotic events which proceed rostrocaudally from non-delaminating to delaminating parts of the field. Apoptosis persists upon regression of the placode, thereby exhibiting a massive "wedge" of apoptotic cells which includes the postulated position of the "ventrolateral postoptic placode" (Lee et al. in Dev Biol 263:176-190, 2003), merges with groups of lens-associated apoptotic cells, and disappears upon lens detachment. In conjunction with earlier work (Washausen et al. in Dev Biol 278:86-102, 2005) our findings suggest that apoptosis contributes repeatedly to the disintegration of the panplacodal primordium, to the elimination of subsets of premigratory placodal neuroblasts, and to the regression of placodes.
Knabe
Wolfgang
W
Department of Anatomy and Embryology, Georg August University, Kreuzbergring 36, 37075 Göttingen, Germany. wknabe@gwdg.de
Obermayer
Bastian
B
Kuhn
Hans-Jürg
HJ
Brunnett
Guido
G
Washausen
Stefan
S
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000196:embryology / Q000502:physiology; D049109:Cell Proliferation; D004475:Ectoderm / Q000254:growth & development / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D009474:Neurons / Q000502:physiology; D010784:Photomicrography; D012668:Trigeminal Ganglion / Q000196:embryology* / Q000502:physiology; D014417:Tupaia / Q000196:embryology*