title: A novel dominant B-cell epitope of FSHR identified by molecular docking induced specific immune response and suppressed fertility.
abstract: The follicle stimulating hormone (FSH) is of great importance in reproduction modulation of both sexes. The extracellular domain (ECD) of its receptor (FSHR) is crucial for FSH binding and subsequent signal transduction; therefore, it is the potential target for fertility control. To avoid unwanted side-effect when used as immunocontraceptive agent, the ECD was analysed by online prediction combined with molecular docking to identify the candidate B-cell epitopes. Four potential B-cell epitopes were identified and synthesised in tandem with Pan DR epitope. Then the epitope-based peptides were used to boost adult male mice following rhFSHR protein priming, thus to determine their immune responses and fertility inhibition capacity. Three of the four peptides showed suppressed fertility accompanied with small testis and lower serum testosterone level, which was consistent with absolutely lower sperm quantity and poor quality. Among the four epitope peptides, Pep2 displayed the lowest fertility rate of 26.67%, which was similar to that of rhFSHR homologously prime/boost mice (23.30 and 25.00%). Thus, we identified a novel immunodominant B-cell epitope by molecular docking and protein prime/peptide boost strategy.
authors.affiliation: Institute of Immunology, Third Military University, Chongqing, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhengqiong
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Shang
authors.forename: Haiyang
authors.initials: H
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Zigang
authors.initials: Z
authors.lastname: Shen
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
authors.forename: Jintao
authors.initials: J
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D003307:Copulation; D004797:Enzyme-Linked Immunosorbent Assay; D004822:Epididymis / Q000276:immunology / Q000378:metabolism; D018985:Epitopes, B-Lymphocyte / Q000276:immunology* / Q000378:metabolism; D005298:Fertility / Q000276:immunology*; D005640:Follicle Stimulating Hormone / Q000276:immunology / Q000378:metabolism; D016056:Immunodominant Epitopes / Q000276:immunology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D011962:Receptors, FSH / Q000276:immunology* / Q000378:metabolism; D013076:Sperm Count; D013194:Staining and Labeling; D013737:Testis / Q000276:immunology / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D014611:Vaccination
chemical_list: D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes; D011962:Receptors, FSH; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone