Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat.
OBJECTIVE
The objective of this study was to histologically analyze the influence of the platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in the calvaria of diabetic rats.


METHODS
A total of 20 diabetic rats were divided into 2 groups: C (control) and PRP. A 5-mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group PRP, 0.35 mL of PRP was placed in the defects. All animals were humanely killed 30 days postoperatively. Histometric and histologic analyses were performed. Data were statistically analyzed (t test, P < .05).


RESULTS
No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C (37.22% +/- 6.00% and 21.68% +/- 11.35%, respectively).


CONCLUSIONS
PRP placed in the defects significantly enhanced bone healing in CSD in the calvaria of diabetic rats both qualitatively and quantitatively.
Mariano
Ronaldo
R
Division of Bucco-Maxillo-Facial Surgery and Traumatology, Department of Clinic and Surgery, Dental School, Federal University of Alfenas - UNIFAL, Alfenas, Brazil. michel_messora@terra.com.br
Messora
Michel
M
de Morais
Adriana
A
Nagata
Maria
M
Furlaneto
Flávia
F
Avelino
Cássia
C
Paula
Fernanda
F
Ferreira
Sabrina
S
Pinheiro
Marcelo
M
de Sene
João Paulo
JP
D000496:Alloxan; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery; D014945:Wound Healing / Q000502:physiology*
D000496:Alloxan