Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30.
The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett-Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.7l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC+BD) productivity of 3.49 g/lh and the yield of 94.67% was obtained.
Zhang
Liaoyuan
L
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Yang
Yunlong
Y
Sun
Jian'an
J
Shen
Yaling
Y
Wei
Dongzhi
D
Zhu
Jiawen
J
Chu
Ju
J
D002072:Butylene Glycols / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D016296:Mutagenesis; D010447:Peptide Hydrolases / Q000737:chemistry; D057057:Serine Proteases / Q000737:chemistry; D012706:Serratia marcescens / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D013927:Thrombosis / Q000378:metabolism
D002072:Butylene Glycols; C026978:2,3-butylene glycol; D005340:Fibrinogen; D010447:Peptide Hydrolases; D057057:Serine Proteases