title: Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase.
abstract: Enzymes catalyze biochemical reactions in highly crowded environments where the amount of macromolecules may occupy up to 40% of the volume. Here we report how cell-like conditions tune catalytic parameters for the monomeric multi-copper oxidase, Saccharomyces cerevisiae Fet3p, in vitro. At low amounts of crowding agent, we detect increases in both of K(M) (weaker substrate binding) and k(cat) (improved catalytic efficiency), whereas at higher crowding levels, both parameters were reduced. Presence of crowding agents does not affect Fet3p structural content but increases thermal resistance. The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics as a result of excluded volume effects making the protein less structurally 'strained'.
authors.affiliation: Department of Chemistry, Chemical Biological Center, Umeå University, 901 87 Umeå, Sweden.
authors.forename: Irina
authors.initials: I
authors.lastname: Pozdnyakova
authors.forename: Pernilla
authors.initials: P
authors.lastname: Wittung-Stafshede
mesh_terms: D020134:Catalytic Domain; D002570:Ceruloplasmin / Q000737:chemistry* / Q000378:metabolism*; D003962:Dianisidine; D004795:Enzyme Stability; D007700:Kinetics; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D017711:Nonlinear Dynamics; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D013053:Spectrophotometry; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; D002570:Ceruloplasmin; C085513:FET3 protein, S cerevisiae; D003962:Dianisidine