Effects of N-acetylcysteine and ebselen on arachidonic acid release from astrocytes and neurons cultured in normoxic or simulated ischemic conditions.
Arachidonic acid (AA) is released from cells after nervous tissue injuries.We treated rat cortical neurons and astrocytes cultured under normoxic or simulated ischemic conditions with N-acetylcysteine (100 or 200 microM) or ebselen (10 or 20 microM). N-acetylcysteine decreased AA release in normoxic astrocytes, while ebselen decreased AA release from astrocytes in both conditions. N-acetylcysteine produced no changes in neuronal AA release. A low dose of ebselen significantly increased AA release from neurons in both conditions. The influence of N-acetylcysteine and ebselen on AA release might be implicated in their effects on astrocytes and neurons, however, the exact mechanisms have yet to be explained.
Pawlas
Natalia
N
Department of Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland.
Nierwińska
Katarzyna
K
Stolecka
Anna
A
Gabryel
Bozena
B
Pudełko
Anna
A
Małecki
Andrzej
A
D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000378:metabolism*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001393:Azoles / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007511:Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D054833:Isoindoles; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D016718:Arachidonic Acid; C042986:ebselen; D000111:Acetylcysteine