title: Analysis of tissue-specific gene expression using laser capture microdissection.
abstract: Epithelial tissues exhibit optimal conditions for studying cellular differentiation since the differentiation status of a single cell can be determined by its distance to the basal membrane. For that reason Laser Capture Microdissection (LCM) may serve as a perfect tool to compare the characteristics of cells that have been collected from different strata of the epithelium. However, as cell boundaries are not visible in untreated tissue sections, samples have to be stained to allow for sufficient structural orientation. This usually results in a considerable reduction of RNA content in the dissected specimen. To circumvent this problem, we have established a modified hematoxylin/eosin staining protocol that concurrently allows visualization of important structures and the subsequent isolation of sufficient RNA amounts to be used for linear amplification and quantitative analyses.
authors.affiliation: Beiersdorf AG, R&D, Skin Research Center, Hamburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Ruetze
authors.forename: Katrin
authors.initials: K
authors.lastname: Lochner
authors.forename: Stefan
authors.initials: S
authors.lastname: Gallinat
authors.forename: Anja
authors.initials: A
authors.lastname: Knott
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006416:Hematoxylin / Q000737:chemistry; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D009928:Organ Specificity; D012313:RNA / Q000032:analysis; D016707:Tissue Fixation
chemical_list: D012313:RNA; D006416:Hematoxylin