title: Peptide binding to MHC class I and II proteins: new avenues from new methods.
abstract: Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic properties such as peptide binding and intracellular trafficking and their immunological (as well as non-immunological) functions are still being intensely investigated. In recent years, new methods and technologies have emerged to detect and characterize the conformational changes and intermediate states that accompany peptide binding and exchange by MHC proteins. These techniques have delivered more detailed information and allowed us to compare the molecular mechanisms of peptide selection between MHC class I and II proteins, suggesting both similarities and differences. Here, we review these recent achievements and suggest avenues for further work.
authors.affiliation: Biochemistry and Cell Biology, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Rakina
authors.initials: R
authors.lastname: Yaneva
authors.forename: Clemens
authors.initials: C
authors.lastname: Schneeweiss
authors.forename: Martin
authors.initials: M
authors.lastname: Zacharias
authors.forename: Sebastian
authors.initials: S
authors.lastname: Springer
mesh_terms: D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D011487:Protein Conformation
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010455:Peptides