[Comparison of the serum proteomes of pathological stages during hepatocarcinogenesis].
OBJECTIVE
To compare the 2-DE profiles for serum proteins of different pathological stages during hepatocarcinogenesis.


METHODS
Sera from hepatocellular carcinoma patients, cirrhosis patients, chronic hepatitis patients and healthy controls were collected. After sonication, albumin and immunoglobulin (IgG) depletion, and desalination, sera were subjected to 2-DE, the differential protein spots were identified by MALDI-TOF-MS. Western blot was used to validate these differentially expressed proteins.


RESULTS
2-DE sera protein profiles were obtained from the patient suffering from HCC, liver cirrhosis, chronic hepatitis, healthy controls in each group. From optimized 2-DE gel images of the above groups, 96 protein spots with more than 2-fold difference in intensity between the two groups were selected by image master 6.0 software, differential proteins including haptoglobin, SAA1 and SP40 were identified by MALDI-TOF-MS/MS. 7 different spots within more than 30 protein spots belonged to the same haptoglobin family. The differential expression of haptoglobin was confirmed by western blot.


CONCLUSIONS
Four protein expression patterns have been identified during the pathological stages of hepatocarcinogenesis. Haptoglobin is significantly increased from liver cirrhosis to HCC. It implies that haptoglobin might be a potential biomarker in the early diagnosis of liver cancer.
Shu
Hong
H
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Kang
Xiao-nan
XN
Li
Mei
M
Guo
Kun
K
Sun
Lu
L
Li
Shan
S
Xie
Li
L
Deng
Jing-huan
JH
Qin
Xue
X
Liu
Yin-kun
YK
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D006521:Hepatitis, Chronic / Q000097:blood / Q000473:pathology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000473:pathology; D008113:Liver Neoplasms / Q000097:blood* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D055815:Young Adult
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D006242:Haptoglobins; D020543:Proteome