[Cytotoxicity evaluation of three kinds of perforation repair materials on human periodontal ligament fibroblasts in vitro].
OBJECTIVE
To select three kinds of perforation repair materials, mineral trioxide aggregate (MTA), Z350, amalgam. And to evaluate the cytotoxicity of three kinds of perforation repair materials on human periodontal ligament fibroblasts (HPDLF) in vitro.


METHODS
The proliferation of HPDLF to three perforation repair materials were examined by methyl thiazolyl tetrazolium (MTT) assay at 1, 3 and 5 days. The mRNA expression levels of bone-associated alkaline phosphatase (ALP) and osteocalcin (OC) were determined using a real-time quantitative polymerase chain reaction (PCR).


RESULTS
MTA shew almost no inhibition to HPDLF, the expression of ALP mRNA and OC mRNA in the HPDLF cultured on MTA were higher. Z350 induced a slight inhibition to HPDLF, and the expression of ALP mRNA but there was no difference in the expression of OC mRNA. Cell proliferation was significantly impaired by amalgam with grade 3, and the expression of ALP mRNA and OC mRNA were significantly reduced.


CONCLUSIONS
MTA have minimum cytotoxicity on HPDLF and can promote cell differentiation and regenerate of periodontal tissue. Z350 have lower cytotoxicity on HPDLF. Amalgam show highest cytotoxicity on HPDLF in the three materials and inhibit cells differentiation.
Wang
Li
L
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Yin
Shi-hai
SH
Zhong
Su-lan
SL
Jie
You-qiong
YQ
D000180:Acrylic Resins; D017607:Aluminum Compounds; D017610:Calcium Compounds; D002454:Cell Differentiation; D002478:Cells, Cultured; D004338:Drug Combinations; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D015675:Osteocalcin; D010087:Oxides; D010513:Periodontal Ligament; D012387:Root Canal Filling Materials; D017640:Silicates
D000180:Acrylic Resins; D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; C073910:Repair Material; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D015675:Osteocalcin