Subunits of the chaperonin CCT interact with F-actin and influence cell shape and cytoskeletal assembly.
The integrity of the cytoskeleton is closely linked to the oligomeric chaperonin containing TCP-1 (CCT) via the folding requirements of actin and tubulin, but the role of CCT in cytoskeletal organization remains unclear. We address this issue by analyzing the effects of targeting CCT subunits via siRNA and assessing their location/assembly state in cultured mammalian cells. Reducing levels of individual CCT subunits implicates CCTepsilon in influencing cell shape and reduced levels of this subunit limit the cells' ability to recover from microfilament depolymerization. Conversely, cells displayed enhanced microtubule regrowth when CCT subunit levels were altered by siRNA. Some CCT subunits co-localize with F-actin, whilst all are predominantly monomeric in extracts enriched for the cytoskeleton. This provides compelling evidence that some CCT subunits as monomers can influence cytoskeletal organization/polymerization. Therefore the activity of CCT may well extend beyond the folding of newly synthesized polypeptides, representing a novel function for CCT subunits distinct from their role in the CCT oligomer.
Brackley
Karen I
KI
Department of Cell and Molecular Biology, Göteborgs Universitet, Medicinaregatan 9C, 405 30 Göteborg, Sweden.
Grantham
Julie
J
D000199:Actins / Q000378:metabolism*; D000818:Animals; D041702:BALB 3T3 Cells; D048430:Cell Shape; D002478:Cells, Cultured; D056404:Chaperonin Containing TCP-1 / Q000235:genetics / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D021122:Protein Subunits
D000199:Actins; D021122:Protein Subunits; D056404:Chaperonin Containing TCP-1