Removal of species constraints in antibody detection.
Serum antibodies from myriad species, particularly birds, can provide key information regarding the transmission and the expansion of the territory of emerging pathogens. Expedient antibody analysis is constrained by a lack of species-specific reagents, a deficiency potentially highlighted by the recent swine-origin influenza A virus (H1N1) outbreak. Available methodologies present difficulties that discourage thorough serologic monitoring of potential disease vectors or hosts. Rapid high-throughput procedures that combined serum amine labeling via biotinylation, contaminant removal, and microsphere-based immunoassays for antibodies to three arboviruses were developed. Agent-specific adaptations of this simple format should facilitate expanded surveillance and diagnostic capabilities regarding pathogens of human and veterinary importance.
Basile
Alison Jane
AJ
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd., Fort Collins, CO 80521, USA. ajj1@cdc.gov
Biggerstaff
Brad J
BJ
Kosoy
Olga L
OL
Junna
Shilpa R
SR
Panella
Nicholas A
NA
Powers
Ann M
AM
Stark
Lillian M
LM
Nemeth
Nicole M
NM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology*; D004665:Encephalitis Virus, St. Louis / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral