In vitro proliferation of human osteogenic cells in presence of different commercial bone substitute materials combined with enamel matrix derivatives.
BACKGROUND
Cellular reactions to alloplastic bone substitute materials (BSM) are a subject of interest in basic research. In regenerative dentistry, these bone grafting materials are routinely combined with enamel matrix derivatives (EMD) in order to additionally enhance tissue regeneration.


METHODS
The aim of this study was to evaluate the proliferative activity of human osteogenic cells after incubation over a period of seven days with commercial BSM of various origin and chemical composition. Special focus was placed on the potential additional benefit of EMD on cellular proliferation.


RESULTS
Except for PerioGlas, osteogenic cell proliferation was significantly promoted by the investigated BSM. The application of EMD alone also resulted in significantly increased cellular proliferation. However, a combination of BSM and EMD resulted in only a moderate additional enhancement of osteogenic cell proliferation.


CONCLUSIONS
The application of most BSM, as well as the exclusive application of EMD demonstrated a positive impact on the proliferation of human osteogenic cells in vitro. In order to increase the benefit from substrate combination (BSM + EMD), further studies on the interactions between BSM and EMD are needed.
Reichert
Christoph
C
Department of Oral and Maxillofacial Surgery, University Hospital Aachen, Aachen, Germany. c_reichert@web.de
Al-Nawas
Bilal
B
Smeets
Ralf
R
Kasaj
Adrian
A
Götz
Werner
W
Klein
Marcus O
MO
D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010078:Oxazines; D014966:Xanthenes
D018786:Bone Substitutes; D003746:Dental Enamel Proteins; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C109069:enamel matrix proteins; C005843:resazurin