title: In Vitro antioxidant and xanthine oxidase inhibitory activities of methanolic Swietenia mahagoni seed extracts.
abstract: This study examines the in vitro antioxidant activities of the methanol extract of Swietenia mahagoni seeds (SMCM seed extract). The extract was screened for possible antioxidant activities by free radical scavenging activity (DPPH), xanthine oxidase inhibition (XOI), hydrogen peroxide scavenging activity (HPSA) and ferric-reducing antioxidant power (FRAP) assays. The total phenolic and flavonoid contents were also determined. The extract exhibits antioxidant activity of 23.29% with an IC(50 )value of 2.3 mg/mL in the DPPH radical scavenging method, 47.2% in the XOI assay, 49.5% by the HPSA method, and 0.728 mmol/Fe(II)g in the FRAP method at the concentration tested. The amount of total phenolics and flavonoid contents was 70.83 mg gallic acid equivalent (GAE) and 2.5 +/- 0.15 mg of catechin equivalent per gram of dry extract, respectively. High Performance Thin Layer Chromatography (HPTLC) screening indicates the presence of phenolic compounds in the SMCM seed extract. The results indicate that the extract has both high free radical scavenging and xanthine oxidase inhibition activity. The antioxidant activity of SMCM seed extract is comparable with that of other Malaysian tropical fruits and herbal plants.
authors.affiliation: Centre for Drug Research, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Geethaa
authors.initials: G
authors.lastname: Sahgal
authors.forename: Surash
authors.initials: S
authors.lastname: Ramanathan
authors.forename: Sreenivasan
authors.initials: S
authors.lastname: Sasidharan
authors.forename: Mohd Nizam
authors.initials: MN
authors.lastname: Mordi
authors.forename: Sabariah
authors.initials: S
authors.lastname: Ismail
authors.forename: Sharif Mahsufi
authors.initials: SM
authors.lastname: Mansor
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D029582:Meliaceae / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014969:Xanthine Oxidase; D000432:Methanol