[Amelogenin expression in cultured human dental pulp and periodontium related cells].
OBJECTIVE
To investigate gene expression of amelogenin (Am) in human gingival epithelial cells(HGEC) and also other oral ectomesenchyme cells (human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells).


METHODS
The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.


RESULTS
There was no amelogenin expression detected in any of the cells.


CONCLUSIONS
These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China (Grant No.30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).
Li
Xi-ting
XT
Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China. sheatinlee@hotmail.com
Shu
Rong
R
Wen
Hai-yan
HY
Cheng
Lan
L
Jiang
Shao-yun
SY
D053523:Amelogenin; D002460:Cell Line; D002478:Cells, Cultured; D003782:Dental Pulp; D004847:Epithelial Cells; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D010513:Periodontal Ligament; D010519:Periodontium
D053523:Amelogenin