title: [Effect of SiO2 on expression of MMP-9/TIMP-1 in human alveolar macrophages in vitro].
abstract: OBJECTIVE
  To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human silicotic alveolar macrophages (AM).
  
  
  METHODS
  Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3 h, 6 h, 12 h, 18 h, 24 h and 36 h. The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.
  
  
  RESULTS
  The expressions of MMP-9 in the AM increased clearly along with the time, reached peak at 24 h when detected with zymography (average optical density: 3.061+/-0.153 vs 2.851+/-0.164, P<0.05); and reached peak at 18h when detected with immunological method (average optical density: 0.386+/-0.037 vs 0.322+/-0.034, P<0.05). The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P>0.05).
  
  
  CONCLUSIONS
  SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.
authors.affiliation: Tianjin Union Medicine Centre, Tianjin 300121, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Hao
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Pei
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000473:pathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
chemical_list: C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D012822:Silicon Dioxide; D020780:Matrix Metalloproteinase 9