Differential effects of growth differentiation factor-5 on porcine dental papilla- and follicle-derived cells.
In this study, the effect of growth differentiation factor-5 (GDF-5) on the growth and differentiation of porcine dental papilla- and follicle-derived cells was investigated. Furthermore, the effect was compared with that of BMP-2. Recombinant mouse GDF-5 (rmGDF-5) enhanced alkaline phosphatase (ALP) activity in dental papilla-derived cells in a dose-dependent manner, while ALP activity in dental follicle-derived cells was reduced. In rmGDF-5 stimulated dental papilla-derived cells, the expressions of odontoblast-marker genes were up-regulated. Conversely, recombinant human BMP-2 (rhBMP-2) enhanced ALP activity dose-dependently in both dental papilla- and follicle-derived cells. When combined, GDF-5 did not further enhance BMP-2-induced ALP activities. Rather, GDF-5 reduced BMP-2-induced ALP activities in both dental papilla- and follicle-derived cells. This suggests that affinity of GDF-5 to the shared receptors may be higher than that of BMP-2 in both cell types. These observations indicate that GDF-5 regulates differentiation of both dental papilla and follicle during odontogenesis, co-operatively with other growth factors such as BMP-2.
Sumita
Yoshinori
Y
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Honda
Masaki J
MJ
Ueda
Minoru
M
Asahina
Izumi
I
Kagami
Hideaki
H
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003795:Dental Sac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055428:Growth Differentiation Factor 5 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D013552:Swine
D055396:Bone Morphogenetic Protein 2; C525894:Gdf5 protein, mouse; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase