title: Formation of well-oriented microtubules with preferential polarity in a confined space under a temperature gradient.
abstract: Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
authors.affiliation: Department of Biological Science, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kakugo
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shikinaka
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoshida
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Kawamura
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Furukawa
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Osada
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Gong
mesh_terms: D016880:Anisotropy; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D032701:Biomimetics; D019277:Entropy; D005898:Glass; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry; D016547:Kinesins / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D008870:Microtubules / Q000737:chemistry* / Q000648:ultrastructure; D013696:Temperature; D014404:Tubulin / Q000737:chemistry*
chemical_list: D014404:Tubulin; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D016547:Kinesins