Isotope dilution method for the assay of rotenone in olive oil and river waters by liquid chromatography/multiple reaction monitoring tandem mass spectrometry.
A new approach for the assay of rotenone (1) by isotope dilution mass spectrometry is presented. The extreme toxicity of rotenone, a natural phytodrug with insecticidal and piscicidal activity, recently banned by national and international environmental protection agencies, calls for the development of sensitive and accurate methods of analyses. Accordingly, the proposed protocol is based on the availability of the labeled internal standard rotenone-d3 (3) which can be conveniently prepared by consecutive and specific mono-O-demethylation, and remethylation with methyl iodide-d3. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and quantitation (LOQ) values achieved in the assay of 1 in two distinct fortified matrices, and is further supported by the observed accuracy values.
Mazzotti
Fabio
F
Dipartimento di Chimica, Università della Calabria, via P. Bucci cubo 12/C I-87030 Arcavacata di Rende (CS), Italy.
Di Donna
Leonardo
L
Attya
Mohamed
M
Gabriele
Bartolo
B
Fazio
Alessia
A
Sindona
Giovanni
G
D002853:Chromatography, Liquid / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D012402:Rotenone / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000069463:Olive Oil; D010938:Plant Oils; D012402:Rotenone