Reduced expression of tocopherol-associated protein (TAP/Sec14L2) in human breast cancer.
We show that TAP/Sec14L2 had a high expression in normal/benign breast, prostate, and liver tissues as compared to lung, colon, and kidney. Its expression was downregulated in breast cancer cell lines shown by quantitative-PCR. Further, 57% of 141 human invasive breast carcinomas had no or markedly reduced TAP/Sec14L2 expression by immunohistochemical staining, and the rate increased to 80% in high grade invasive carcinomas (p < .01). This downregulation of TAP/Sec14L2 was also present in ductal carcinoma in situ (DCIS) associated with invasive carcinomas. These findings raise the possibility that TAP/Sec14L2 may serve as a tumor suppressor in breast carcinogenesis.
Wang
Xi
X
Department of Pathology and Laboratory Medicine, University of Rochester Medical School, Rochester, New York, USA.
Ni
Jing
J
Hsu
Chen-Long
CL
Johnykutty
Sharlin
S
Tang
Ping
P
Ho
Yuan-Soon
YS
Lee
Chia-Hwa
CH
Yeh
Shuyuang
S
D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D008074:Lipoproteins; D012333:RNA, Messenger; C432422:SEC14L2 protein, human; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins