title: Gas chromatography/atmospheric pressure chemical ionization-time of flight mass spectrometry: analytical validation and applicability to metabolic profiling.
abstract: Gas Chromatography (GC)-Mass Spectrometry (MS) with Atmospheric Pressure (AP) interface was introduced more than 30 years ago but never became a mainstream technique, mainly because of technical difficulties and cost of instrumentation. A recently introduced multipurpose AP source created the opportunity to reconsider the importance of AP ionization for GC. Here, we present an analytical evaluation of GC/APCI-MS showing the benefits of soft atmospheric pressure chemical ionization for GC in combination with a Time of Flight (TOF) mass analyzer. During this study, the complete analytical procedure was optimized and evaluated with respect to characteristic analytical parameters, such as repeatability, reproducibility, linearity, and detection limits. Limits of detection (LOD) were found within the range from 11.8 to 72.5 nM depending on the type of compound. The intraday and interday repeatability tests demonstrate relative standard deviations (RSDs) of peak areas between 0.7%-2.1% and 3.8%-6.4% correspondingly. Finally, we applied the developed method to the analysis of human cerebrospinal fluid (CSF) samples to check the potential of this new analytical combination for metabolic profiling.
authors.affiliation: Biomolecular Mass Spectrometry Unit, Department of Parasitology, LUMC, Leiden, The Netherlands.
authors.forename: Alegría
authors.initials: A
authors.lastname: Carrasco-Pancorbo
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Nevedomskaya
authors.forename: Thomas
authors.initials: T
authors.lastname: Arthen-Engeland
authors.forename: Thomas
authors.initials: T
authors.lastname: Zey
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zurek
authors.forename: Carsten
authors.initials: C
authors.lastname: Baessmann
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Mayboroda
mesh_terms: D001274:Atmospheric Pressure; D002555:Cerebrospinal Fluid / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015203:Reproducibility of Results; D013997:Time Factors