title: Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection.
abstract: Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C(1)-C(4), C(2)-C(5) and C(3)-C(6) form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins. In non-stimulated abalone, defensin transcripts were constitutively expressed in all examined tissues including hemocytes, gills, mantle, muscle, digestive tract and hepatopancreas. It was observed that abalone defensin transcripts were significantly induced in hemocytes, gills and digestive tract at different time intervals after infection by pathogenic bacteria mixture containing Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes. Our overall results suggest that disk abalone defensin could be involved in the immune response reactions as a host defense against pathogenic bacteria.
authors.affiliation: Department of Marine Life Sciences, College of Ocean Sciences, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea.
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Ilson
authors.initials: I
authors.lastname: Whang
authors.forename: Youngdeuk
authors.initials: Y
authors.lastname: Lee
authors.forename: Sukkyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D023082:Defensins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D041981:Gastrointestinal Tract / Q000276:immunology; D049851:Gastropoda / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation; D005880:Gills / Q000276:immunology; D006434:Hemocytes / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D023082:Defensins