Proteomic analysis of early phase of conidia germination in Aspergillus nidulans.
In order to investigate proteins involved in early phase of conidia germination, proteomic analysis was performed using two-dimensional gel electrophoresis (2D-GE) in conjunction with MALDI-TOF mass spectrometry (MS). The expression levels of 241 proteins varied quantitatively with statistical significance (P<0.05) at the early phase of the germination stage. Out of these 57 were identified by MALDI-TOF MS. Through classification of physiological functions from Conserved Domain Database analysis, among the identified proteins, 21, 13, and 6 proteins were associated with energy metabolism, protein synthesis, and protein folding process, respectively. Interestingly, eight proteins, which are involved in detoxification of reactive oxygen species (ROS) including catalase A, thioredoxin reductase, and mitochondrial peroxiredoxin, were also identified. The expression levels of the genes were further confirmed using Northern blot and reverse transcriptase (RT)-PCR analyses. This study represents the first proteomic analysis of early phase of conidia germination and will contribute to a better understanding of the molecular events involved in conidia germination process.
Oh
Young Taek
YT
Department of Microbiology and Reserch Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
Ahn
Chun-Seob
CS
Kim
Jeong Geun
JG
Ro
Hyeon-Su
HS
Lee
Chang-Won
CW
Kim
Jae Won
JW
D001233:Aspergillus nidulans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D005656:Fungal Proteins; D008565:Membrane Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D054464:Peroxiredoxins