Molecular characterization of a human group C rotavirus detected first in Turkey.
The present study was done to find out the prevalence of group B and C rotavirus infections in children with diarrhea presented at two major hospitals in Ankara, Turkey. Group B rotavirus was not found in any samples. One of 122 samples was positive for group C rotavirus. Phylogenetic analysis of genes for nonstructural protein NSP4, and structural proteins VP4, VP6, and VP7 confirmed the human origin of this strain. Similar to other human group C rotaviruses, one N-glycosylation site was predicted at amino acid residue 67 on the VP7 of strain GUP188. The genes of strain GUP188 were closely related to those of human group C rotavirus strain from the UK (Bristol) for NSP4, China (208 and Wu82) for VP4 and VP6, and from Colombia (Javeriana) for VP7, indicating that the Turkish group C rotavirus was unique and can serve as an additional reference strain for the molecular epidemiology of group C rotaviruses.
Mitui
Marcelo Takahiro
MT
Department of Microbiology, School of Medicine, Oita University, Yufu, Oita, Japan.
Bozdayi
Gulendam
G
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Dalgic
Buket
B
Department of Pediatric Gastroenterology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Bostanci
Ilknur
I
Department of Pediatrics, Ministry of Health Ankara Training and Education Hospital, Ankara, Turkey.
Nishizono
Akira
A
Department of Microbiology, School of Medicine, Oita University, Yufu, Oita, Japan.
Ahmed
Kamruddin
K
Division of Infectious Diseases, Department of Social and Environmental Medicine, Institute of Scientific Research, Oita University, Yufu, Oita, Japan. ahmed@med.oita-u.ac.jp.
D002675:Child, Preschool; D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D014421:Turkey / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins