Detection of H6 influenza antibody by blocking enzyme-linked immunosorbent assay.
Low pathogenic H6N1 avian influenza viruses (AIVs) have circulated in domestic chickens since 1972 in Taiwan. Detection of avian influenza (AI) antibody is a routine work for AI control in Taiwan. The currently available commercial enzyme-linked immunosorbent assays (ELISAs) are unable to differentiate antibody responses between different subtypes. To this end, a panel of monoclonal antibodies (mAbs) to A/chicken/Taiwan/2838V/00 (H6N1) was developed and implemented a blocking ELISA (bELISA) to detect the H6 antibody. These mAbs were confirmed specific to H6 AIVs. One monoclonal antibody was purified and labeled with horseradish peroxidase to set up a bELISA. The cut-off value was calculated to be 30% inhibition percentage from 138 H6-negative sera. The sensitivity and specificity of the bELISA were 100% and 97%, respectively. The bELISA detected seroconversion in H6-infected farms earlier than hemagglutination inhibition test. The results show that this bELISA could be used to detect H6 infection in the field.
Chen
Yi-Tung
YT
School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Juang
Rong-Huay
RH
He
Jie-Long
JL
Chu
Wen-Yu
WY
Wang
Ching-Ho
CH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013624:Taiwan
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral