title: Comparative validation study to demonstrate the equivalence of a minor modification to AOAC Method 996.09 Visual Immunoprecipitate (VIP) for E. coli O157:H7 method to the reference culture method.
abstract: The Visual Immunoprecipitate (VIP) for the Detection of Escherichia coli 0157:H7 in Foods, AOAC Official Method 996.09, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, there were valid results from 225 samples and controls. Results showed that the VIP Gold for E. coli O157:H7 is equivalent to the reference culture methods for the detection of E. coli O157:H7.
authors.affiliation: BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA. ptf@biocontrolsys.com
authors.forename: Philip T
authors.initials: PT
authors.lastname: Feldsine
authors.forename: David E
authors.initials: DE
authors.lastname: Kerr
authors.forename: George
authors.initials: G
authors.lastname: Shen
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Lienau
mesh_terms: D000818:Animals; D001628:Beverages; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005506:Food Contamination; D019649:Food Industry; D005516:Food Microbiology / Q000592:standards*; D047468:Immunoprecipitation / Q000379:methods*; D008460:Meat; D012016:Reference Values; D015203:Reproducibility of Results; D018724:Spinacia oleracea
chemical_list: D003470:Culture Media