title: An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments.
abstract: OBJECTIVE
  To compare the effects of 2 different media on embryo morphology and development at days 2/3.
  
  
  METHODS
  Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n = 286) or ICSI (n = 390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates.
  
  
  RESULTS
  Fertilization rates in GIII series and ISM (IVF: 59.9 vs 62.0% and ICSI: 65.7 vs 66.8%) respectively were not different. GIII series showed an increase, compared to ISM, of early cleavage rate, (IVF: 25.8 vs 16.2% (p = 0.005); ICSI: 40.8 vs 25.5% (p < 0.0001), and good embryo morphology rate at day 2 [IVF: 64.6 vs 57.3% (p = 0.01); ICSI: 74.2 vs 69.4 (p = 0.03)] and at day 3 [IVF: 57.5 vs 49.0% (p = 0.02); ICSI: 67.2 vs 61.6% (p = 0.01)].
  
  
  CONCLUSIONS
  Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.
authors.affiliation: Service d'Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction, Centre Hospitalier Universitaire Jean Verdier, Assistance Publique-Hôpitaux de Paris, 93143 Bondy, France. christophe.sifer@jvr.aphp.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Sifer
authors.forename: Deborah
authors.initials: D
authors.lastname: Handelsman
authors.forename: Emilie
authors.initials: E
authors.lastname: Grange
authors.forename: Raphael
authors.initials: R
authors.lastname: Porcher
authors.forename: Christophe
authors.initials: C
authors.lastname: Poncelet
authors.forename: Brigitte
authors.initials: B
authors.lastname: Martin-Pont
authors.forename: Brigitte
authors.initials: B
authors.lastname: Benzacken
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Wolf
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
chemical_list: D003470:Culture Media