Measurement of vitamin D in foods and nutritional supplements by liquid chromatography/tandem mass spectrometry.
Vitamin D is a fat-soluble vitamin with great nutritional interest. An HPLC/MS/MS method was developed to measure vitamin D with atmospheric pressure chemical ionization. Under the experimental parameters used, the LOQ was 0.018 IU/g or 0.45 ng/g, which greatly enhances the capability of measurement of vitamin D at low levels in foods and supplements. This method was validated with spike recovery of 100 +/- 15% and the RSD of less than 10% for most sample matrixes, including infant formula, cheese, cereal and cereal-based foods, multivitamin supplements, and pet foods. The results for vitamin D were compared with those obtained by other methods.
Huang
Min
M
Covance Laboratories Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA.
LaLuzerne
Paul
P
Winters
Doug
D
Sullivan
Darryl
D
D000818:Animals; D002138:Calibration; D002611:Cheese; D002762:Cholecalciferol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D019587:Dietary Supplements; D002523:Edible Grain; D004872:Ergocalciferols / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014807:Vitamin D / Q000032:analysis*; D014815:Vitamins / Q000737:chemistry
D004872:Ergocalciferols; D014815:Vitamins; D014807:Vitamin D; D002762:Cholecalciferol