title: MEPE evolution in mammals reveals regions and residues of prime functional importance.
abstract: In mammals, the matrix extracellular phosphoglycoprotein (MEPE) is known to activate osteogenesis and mineralization via a particular region called dentonin, and to inhibit mineralization via its ASARM (acidic serine-aspartate rich MEPE-associated motif) peptide that also plays a role in phosphatemia regulation. In order to understand MEPE evolution in mammals, and particularly that of its functional regions, we conducted an evolutionary analysis based on the study of selective pressures. Using 37 mammalian sequences we: (1) confirmed the presence of an additional coding exon in most placentals; (2) highlighted several conserved residues and regions that could have important functions; (3) found that dentonin function was recruited in a placental ancestor; and (4) revealed that ASARM function was present earlier, pushing the recruitment of MEPE deep into amniote origins. Our data indicate that MEPE was involved in various functions (bone and eggshell mineralization) prior to acquiring those currently known in placental mammals.
authors.affiliation: UMR 7138, Equipe Evolution & Développement du Squelette Université Paris 6, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Bardet
authors.forename: Sidney
authors.initials: S
authors.lastname: Delgado
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Sire
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002415:Cats; D002417:Cattle; D004285:Dogs; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D012641:Selection, Genetic; D016415:Sequence Alignment
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C486579:MEPE protein, human; D010750:Phosphoproteins