The stoichiometric relationship between KCNH-2 and KCNE-2 in I(Kr) channel formation.
KCNH-2 and KCNE-2 may encode the channel-forming alpha- and regulatory beta-subunits, respectively, of I(Kr) channels, which are involved in inherited or acquired long QT syndrome. However, in contrast to other multimeric channels, the stoichiometry of KCNH-2 and KCNE-2, which should be reasonably maintained if they are to be accepted as the components of a multi-molecular complex, has not been established, yet. In this study, we found that the protein expression of KCNE-2 was adequate to support the formation of a complex with KCNH-2; however, the level of transcription was not. This finding, together with previous data from electrophysiological and molecular biological studies, supports that KCNH-2 and KCNE-2 are molecular components of I(Kr) channels.
Kim
Do-Geun
DG
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.
Oh
Jung-Hwa
JH
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.
Lee
Eun-Hee
EH
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea; Pharmacology and Toxicology, School of Engineering, University of Science and Technology, 113 Gwahangno, Yuseong, Daejeon, 305-333, Republic of Korea.
Lee
Jong-Hwa
JH
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.
Park
Han-Jin
HJ
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.
Kim
Choong-Yong
CY
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.
Kwon
Myung-Sang
MS
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea; School of Veterinary Medicine, Kangwon National University, Chunchon, Republic of Korea.
Yoon
Seokjoo
S
Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea; Pharmacology and Toxicology, School of Engineering, University of Science and Technology, 113 Gwahangno, Yuseong, Daejeon, 305-333, Republic of Korea. Electronic address: sjyoon@kitox.re.kr.
D000818:Animals; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels / Q000096:biosynthesis / Q000737:chemistry*; D024642:Potassium Channels, Voltage-Gated / Q000096:biosynthesis / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C489621:KCNE2 protein, rat; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; C059983:potassium channel protein I(sk)