Surface plasmon resonance (SPR) as a rapid tool for serotyping of Salmonella.
An SPR-based sandwich immunoassay for serotyping of Salmonella is demonstrated. The Salmonella are captured on an SPR chip using polyclonal capture antibody. SPR sensorgrams are obtained for the immunoreactions of the somatic (O) and flagellar (H) surface antigens, of the captured bacteria, to their respective antibodies. The sensorgram data are compiled to determine the antigenic formula in accordance with the Kauffmann-White scheme. Salmonella Enteritidis was completely serotyped using this SPR-based method. In addition, Salmonella belonging to serogroups B, C and D were successfully assigned to their respective serogroups. Before serotyping the bacteria are grown to a concentration of 1x10(10) mL(-1). This SPR-based serotyping provides quantitative data, and thus, eliminates the possibility of false detections as encountered in the conventional slide agglutination test (SAT). This method was also proved to work with rough strains.
Mazumdar
Saikat Datta
SD
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Germany.
Barlen
Benjamin
B
Kämpfer
Peter
P
Keusgen
Michael
M
D015374:Biosensing Techniques / Q000295:instrumentation*; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*