Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs.
An IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay were developed for rapid screening of leptospiral antibodies in dogs using recombinant LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires as the coating antigen. Results from this study were compared with the standard microscopic agglutination test and found that the sensitivity and specificity of the enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow-through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative merits of these tests were also assessed. The flow-through-based dot-immunobinding assay was thus proved to be a valid screening test for canine leptospirosis.
Subathra
M
M
Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, India.
Senthilkumar
T M A
TM
Ramadass
P
P
Dhinakar Raj
G
G
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D007118:Immunoassay / Q000592:standards; D007919:Leptospira / Q000502:physiology; D007922:Leptospirosis / Q000175:diagnosis / Q000662:veterinary*; D008297:Male; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D000942:Antigens, Bacterial; C538745:LipL41 protein, Leptospira interrogans; D011993:Recombinant Fusion Proteins