Upregulation of SEPT7 gene inhibits invasion of human glioma cells.
OBJECTIVE
To explore the role of SEPT7 in glioma cell invasion.


METHODS
SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin alpha(v)beta(3), and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of alpha-tubulin was examined by laser scanning confocal analysis.


RESULTS
After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin alpha(v)beta(3) was decreased, while TIMP1/2 was increased, and alpha-tubulin was redistributed.


CONCLUSIONS
These results suggest that SEPT7 plays an important role in the glioma cell invasion.
Xu
Song
S
Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China.
Jia
Zhi-Fan
ZF
Kang
Chunsheng
C
Huang
Qiang
Q
Wang
Guangxiu
G
Liu
Xiaozhi
X
Zhou
Xuan
X
Xu
Peng
P
Pu
Peiyu
P
D000818:Animals; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005909:Glioblastoma / Q000473:pathology; D005910:Glioma / Q000473:pathology*; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D058112:Septins; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D014162:Transfection; D014404:Tubulin / Q000032:analysis; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D014404:Tubulin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14; D019204:GTP-Binding Proteins; C113158:SEPTIN7 protein, human; D058112:Septins