Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity.
In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.
Gong
Chang-Yang
CY
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, and School of Life Sciences, Sichuan University, Chengdu, 610041, P.R. China.
Shi
Shuai
S
Peng
Xin-Yun
XY
Kan
Bing
B
Yang
Li
L
Huang
Mei-Juan
MJ
Luo
Feng
F
Zhao
Xia
X
Wei
Yu-Quan
YQ
Qian
Zhi-Yong
ZY
D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000633:toxicity; D056724:Immunity, Humoral; D007267:Injections; D007739:L Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D011091:Polyesters / Q000008:administration & dosage / Q000633:toxicity; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000633:toxicity; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011091:Polyesters; D014612:Vaccines; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols