title: A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase.
abstract: A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of [Ru(NH(3))(6)](3+) absorbed on the DNA-AuNPs.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
authors.forename: Xiahong
authors.initials: X
authors.lastname: Xu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Fu
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.forename: Qinpeng
authors.initials: Q
authors.lastname: Shen
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D006046:Gold; D012428:Ruthenium; D004247:DNA; D015040:Zirconium; D011494:Protein Kinases