title: Epithelial Na+ channels derived from human lung are activated by shear force.
abstract: During breathing the pulmonary epithelial cells are permanently exposed to physical forces and shear force (SF) in particular. In our present study we questioned whether the lung epithelial Na(+) channel (hENaC) responds to shear force. For this purpose ENaC was cloned from human lung tissue, expressed in Xenopus oocytes and functionally characterized by electrophysiological techniques. Shear force in physiological relevant ranges was applied via a fluid stream. By the application of SF we obtained an increased inward current indicating an activation of hENaC. The SF-induced effect was reversible and interestingly, the response to SF was augmented by trypsin due to proteolytic cleavage. The direct activation of hENaC by SF was confirmed in outside-out single channel experiments. In five out of nine recordings an increased NP(O) was observed. From our observations we conclude that lung-derived hENaCs are directly activated by SF and this may represent an important feature for the regulation of pulmonary Na(+) reabsorption and pulmonary fluid homeostasis.
authors.affiliation: Institute of Animal Physiology, University of Giessen Lung Center, Justus-Liebig-University, Giessen, Germany. martin.fronius@bio.uni-giessen.de
authors.forename: Martin
authors.initials: M
authors.lastname: Fronius
authors.forename: Roman
authors.initials: R
authors.lastname: Bogdan
authors.forename: Mike
authors.initials: M
authors.lastname: Althaus
authors.forename: Rory E
authors.initials: RE
authors.lastname: Morty
authors.forename: Wolfgang G
authors.initials: WG
authors.lastname: Clauss
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001703:Biophysics; D004558:Electric Stimulation / Q000379:methods; D004847:Epithelial Cells / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000166:cytology*; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D009865:Oocytes; D018408:Patch-Clamp Techniques / Q000379:methods; D010812:Physical Stimulation / Q000379:methods; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013314:Stress, Mechanical; D014162:Transfection / Q000379:methods; D014357:Trypsin / Q000494:pharmacology; D014981:Xenopus
chemical_list: D053503:Epithelial Sodium Channels; D026941:Sodium Channel Blockers; D000584:Amiloride; D014357:Trypsin