title: Performance of TB immunodiagnostic tests in Eurasian badgers (Meles meles) of different ages and the influence of duration of infection on serological sensitivity.
abstract: BACKGROUND
  In parts of Great Britain and Ireland, Eurasian badgers (Meles meles) constitute a reservoir of Mycobacterium bovis infection and a potential source of infection for cattle. In vitro diagnostic tests for live badgers are an important component of strategies to control TB in this species. Immunological tests have been developed for badgers, although little is known about the influence of the age of the animal on test performance. To address this, we evaluated the performance of three immunological tests for badgers with respect to the age of the animal: the Brock Test and BrockTB STAT-PAK serological tests and the recently developed interferon-gamma enzyme immunoassay (IFNgamma EIA). Data published elsewhere suggested that seropositivity was associated with more progressive forms of TB in the badger. To gain further evidence for this, we used longitudinal data from a well-studied population of badgers to test for an association between the sensitivity of the Brock Test and the duration of TB infection.
  
  
  RESULTS
  Sensitivity of the two serological tests was approximately 54% for both cubs and adults. Sensitivity of the IFNgamma EIA was lower in cubs (57%) compared with adults (85%) when a common cut-off value was used to define test positivity. Taking data from the cubs alone, the IFNgamma EIA cut-off value could be adjusted to increase the sensitivity to 71% with no loss in specificity. As a general observation, specificity of all tests was higher in cubs, although only significantly so in the case of the Brock Test. Using logistic regression analysis to adjust for age, sensitivity of the Brock Test was significantly lower at first culture positive event (58%), but increased to >80% as infection progressed.
  
  
  CONCLUSIONS
  These data suggest that serodiagnosis could be a valuable tool for detecting a higher proportion of badgers with the greatest probability of transmitting infection. The age category of the badger appeared to exert little influence on the performance of the serological tests. Although data were only available for the IFNgamma EIA in a small number of cubs, reduced sensitivity of the test in these individuals suggests a lower cut-off may be needed when testing younger animals.
authors.affiliation: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Surrey KT15 3NB, UK. m.a.chambers@vla.defra.gsi.gov.uk
authors.forename: Mark A
authors.initials: MA
authors.lastname: Chambers
authors.forename: Sue
authors.initials: S
authors.lastname: Waterhouse
authors.forename: Konstantin
authors.initials: K
authors.lastname: Lyashchenko
authors.forename: Richard
authors.initials: R
authors.lastname: Delahay
authors.forename: Robin
authors.initials: R
authors.lastname: Sayers
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
mesh_terms: D000367:Age Factors; D000818:Animals; D007159:Immunologic Tests / Q000592:standards / Q000662:veterinary*; D045969:Mustelidae / Q000276:immunology*; D009163:Mycobacterium bovis; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis / Q000662:veterinary*