Preparation of EGFR monoclonal antibody conjugated nanoparticles and targeting to hepatocellular carcinoma.
This study aims to determine the sensitivity, specificity and accuracy of epidermal growth factor receptor monoclonal antibody (EGFRmAb) modified poly(lactic acid-co-l-lysine) nanoparticles (PLA-PLL-EGFRmAb) NPs delivery system to EGFR positive cancer cells. In the study, a new PLA-PLL-EGFRmAb NPs was prepared. The cellular cytotoxicity, cellular uptake, and the targeted effect for hepatocellular carcinoma of PLA-PLL-EGFRmAb NPs were investigated. In vitro, the findings of Flow cytometry and Confocal Laser scanning Biological Microscopy showed that PLA-PLL-EGFRmAb NPs can bind to hepatocellular carcinoma cells and were uptaken effectively. In vivo in the SMMC-7721 xenograft mouse model, PLA-PLL-EGFRmAb NPs could target to the tumor effectively, which demonstrated a better targeting. These results showed that the PLA-PLL-EGFRmAb NPs have the potential to be used as a target delivery carrier for tumor therapies.
Liu
Peifeng
P
State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 200051, People's Republic of China.
Li
Zonghai
Z
Zhu
Mingjie
M
Sun
Ying
Y
Li
Yaogang
Y
Wang
Hongzhi
H
Duan
Yourong
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology*; D045744:Cell Line, Tumor; D004339:Drug Compounding / Q000379:methods; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D053769:Nanocapsules / Q000737:chemistry* / Q000627:therapeutic use*
D000911:Antibodies, Monoclonal; D053769:Nanocapsules; C541485:EGFR protein, mouse; D066246:ErbB Receptors