title: Experiment on the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles.
abstract: The objective of this study was to investigate the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles. Ultrasound (US) combined with phospholipid-based microbubbles (MB) was used to enhance the susceptibility of colon cancer cell line SWD-620 to anticancer drugs Topotecan hydrochloride (TOP). Experiments were designed to investigate the influence of main factors on cell viability and cell inhibition, such as US intensity, MB concentration, drug combination with MB, asynchronous action between US triggered cavitation and drug entering cell, MB particle size. US exposure for 10 sec with US probe power at 0.6 W/cm(2) had satisfied cell viability. Treated with US combined with 15% MB, cell viability maintained more than 85% and cell inhibition 86.16%. Under optimal US combined with MB, TOP showed much higher cell inhibition than that of only TOP group. Cell inhibition under short delayed time (<2 h) for TOP addition did not show obvious difference. In terms of MB particle size, the order of cell inhibition was: Mixture > Micron bubble part > Nanometer bubble part. US combined with MB can enhance the susceptibility of cancer cells to chemotherapeutic drug, which may provide a potential method for US-mediated tumor chemotherapy.
authors.affiliation: Pharmacy School, Wenzhou Medical College, Zhejiang Province, China. lctuua@yahoo.com.cn
authors.forename: Ying-Zheng
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Gao
authors.forename: Zhi-Cai
authors.initials: ZC
authors.lastname: Zhou
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Tang
authors.forename: Cui-Tao
authors.initials: CT
authors.lastname: Lu
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Jin
authors.forename: Ji-Lai
authors.initials: JL
authors.lastname: Tian
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Xu
authors.forename: Xin-Qiao
authors.initials: XQ
authors.lastname: Tian
authors.forename: Lee
authors.initials: L
authors.lastname: Wang
authors.forename: Fan-Lei
authors.initials: FL
authors.lastname: Kong
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Li
authors.forename: Pin-Tong
authors.initials: PT
authors.lastname: Huang
authors.forename: Hui-Liao
authors.initials: HL
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D045423:Microbubbles; D010743:Phospholipids / Q000737:chemistry; D013010:Sonication; D019772:Topotecan / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D014465:Ultrasonics
chemical_list: D000970:Antineoplastic Agents; D010743:Phospholipids; D019772:Topotecan