Chemical fingerprinting of Andrographis paniculata (Burm. f.) Nees by HPLC and hierarchical clustering analysis.
The aim is to develop a simple and specific method for the extraction and chemical fingerprinting of Andrographis paniculata (Burm. f.) Nees and to apply the method to this drug from different regions. High-performance liquid chromatographic (HPLC) with gradient elution is used for developing the fingerprints, and liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) technique is employed to identify the component of the fingerprints. Nine peaks are selected as common peaks, and six compounds are elucidated by MS data. Twenty-three samples of A. paniculata from different regions of China are collected and detected by HPLC fingerprinting. Comparisons of the chromatograms show that there are obvious differences in the content of each component contained between the habitat samples in China. The results of hierarchical cluster analysis show that these samples can be clustered reasonably into three groups, and the growth of A. paniculata and their internal quality are related to their habitat. The HPLC fingerprint developed allows simple identification of A. paniculata from many natural drugs.
Dong
Hai-Juan
HJ
Key laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.
Zhang
Zun-Jian
ZJ
Yu
Jing
J
Liu
Ying
Y
Xu
Feng-Guo
FG
D035685:Andrographis / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D010936:Plant Extracts