title: Isolation of Vibrio alginolyticus and Vibrio splendidus from captive-bred seahorses with disease symptoms.
abstract: Vibrio species isolated from diseased seahorses were characterized by PCR amplification of repetitive bacterial DNA elements (rep-PCR) and identified by 16S ribosomal RNA gene sequence analysis. The results demonstrated that Vibrio alginolyticus and Vibrio splendidus were predominant in the lesions of these seahorses. To our knowledge, this is the first time that these bacterial species have been associated with disease symptoms in captive-bred seahorses.
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, 36208 Vigo, Spain. balcazar@iim.csic.es
authors.forename: José L
authors.initials: JL
authors.lastname: Balcázar
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gallo-Bueno
authors.forename: Miquel
authors.initials: M
authors.lastname: Planas
authors.forename: José
authors.initials: J
authors.lastname: Pintado
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S