Haloterrigena daqingensis sp. nov., an extremely haloalkaliphilic archaeon isolated from a saline-alkaline soil.
A haloalkaliphilic archaeon, strain JX313(T), was isolated from a saline-alkaline soil from Daqing, Heilongjiang Province, China. Its morphological, physiological and biochemical features and 16S rRNA gene sequence were determined. Colonies of the strain were orange-red and cells were non-motile cocci and Gram-stain-variable. The strain required at least 1.7 M NaCl for growth, with optimal growth occurring in 2.0-2.5 M NaCl. Growth was observed at 20-50°C and pH 8.0-10.5, with optimal growth at 35°C and pH 10.0. The G+C content of its genomic DNA was 59.3 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain JX313(T) is associated with the genera Haloterrigena and Natrinema and is most closely related to Haloterrigena salina XH-65(T) (96.2  % sequence similarity) and Haloterrigena hispanica FP1(T) (96.2 %). DNA-DNA hybridization experiments revealed that the relatedness of strain JX313(T) to type strains of related species of the genus Haloterrigena or Natrinema was less than 50 %. Furthermore, the cellular polar lipids of strain JX313(T), identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S₂-DGD), were consistent with the polar lipid characteristics of the genus Haloterrigena. Therefore, phylogenetic analysis, phenotypic assessment and chemotaxonomic data showed that JX313(T) represents a novel species within the genus Haloterrigena, for which the name Haloterrigena daqingensis sp. nov. is proposed. The type strain is JX313(T) (=CGMCC 1.8909(T) =NBRC 105739(T)).
Wang
Shuang
S
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Yang
Qian
Q
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Liu
Zhi-Hua
ZH
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Sun
Lei
L
Soil Fertilizer and Environment Energy Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Wei
Dan
D
Soil Fertilizer and Environment Energy Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Zhang
Jun-Zheng
JZ
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Song
Jin-Zhu
JZ
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Yuan
Hai-Feng
HF
Nature and Ecology Institute, Heilongjiang Academy of Sciences, Harbin 150040, PR China.
D001482:Base Composition; D050260:Carbohydrate Metabolism; D002681:China; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D010743:Phospholipids; D010860:Pigments, Biological; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride