[The effects of TiO2 nano-pore on titanium surface on cell adhesion and spreading of bone marrow stromal cells].
OBJECTIVE
To study the effect of TiO2 nano-pore on titanium surface on cell biological characteristics of bone marrow stromal cells (BMSCs), such as cell adhesion and spreading.


METHODS
TiO2 nano-pore structure on titanium surface with certain shape and size was prepared by electrochemical anodizing and chemical corrosion technologies. The effect of TiO2 nano-pore on titanium surface on cell adhesion, morphology, cytoskeleton protein of BMSCs was detected by laser scanning confocal microscope, scanning electron microscope. The data was analyzed by Student t test using SAS6.12 software package.


RESULTS
TiO2 nano-pores were produced on titanium surface, which was of about 30nm in width and 15nm in depth. More adhesive cells, larger cell spreading area, thinker cytoskeleton, richer fine pseudopodia were observed in the treatment group than in the control group after 4 hours and 8 hours of incubation of BMSCs (P<0.05). The area of BMSCs cell spreading in treatment group was 1.35 times than in the control group (P=0.001).


CONCLUSIONS
TiO2 nano-pores produced on titanium surface by electrochemical anodizing and chemical corrosion technologies may promote cell adhesion and spreading of BMSCs. Supported by National Natural Science Foundation of China(Grant No.30572053), Nanometer Research Project of Shanghai Municipality(Grant No. 0452nm063), Research Fund of Science and Technology Commission of Shanghai Municipality (Grant No.09DZ2271100) and Shanghai Leading Academic Discipline Project (Grant No.S30206).
Zhang
Mei
M
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China. jxb_zhangmei@126.com
Huang
Qi-yu
QY
Zhou
Xiao-jian
XJ
Sun
Qiang
Q
Yang
Fei
F
Chen
Wan-tao
WT
D002448:Cell Adhesion; D059630:Mesenchymal Stem Cells; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium
C009495:titanium dioxide; D014025:Titanium