Characterization of the CtsR stress response regulon in Lactobacillus plantarum.
Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Delta ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR-clpC operon and hsp1.
Fiocco
Daniela
D
Department of Biomedical Sciences, University of Foggia, Viale L. Pinto, 71100 Foggia, Italy.
Capozzi
Vittorio
V
Collins
Michael
M
Gallone
Anna
A
Hols
Pascal
P
Guzzo
Jean
J
Weidmann
Stephanie
S
Rieu
Aurélie
A
Msadek
Tarek
T
Spano
Giuseppe
G
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D006360:Heat-Shock Proteins; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D018085:Regulon / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; C118338:CtsR protein, bacteria; D006360:Heat-Shock Proteins; D012097:Repressor Proteins