Electrochemical selective detection of dopamine on microbial carbohydrate-doped multiwall carbon nanotube-modified electrodes.
Microbial carbohydrate-doped multiwall carbon nanotube (MWNT)-modified electrodes were prepared for the purpose of determining if 4-(2-aminoethyl)benzene-1,2-diol (3,4-dihydroxyphenylalanine; dopamine) exists in the presence of 0.5 mM ascorbic acid, a representative interfering agent in neurotransmitter detection. The microbial carbohydrate dopants were alpha-cyclosophorohexadecaose (alpha-C16) from Xanthomonas oryzae and cyclic-(1 --> 2)-beta-d-glucan (Cys) from Rhizobium meliloti. The cyclic voltammetric responses showed that the highest sensitivity (5.8 x 10(-3) mA cm(-2) microM(-1)) is attained with the Cys-doped MWNT-modified ultra-trace carbon electrode, and that the alpha-C16-doped MWNT-modified glassy carbon electrode displays the best selectivity to dopamine (the approximate peak potential separation is 310 mV).
Jin
Joon-Hyung
JH
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, 143-701, Republic of Korea.
Cho
Eunae
E
Jung
Seunho
S
D002021:Buffers; D002138:Calibration; D004298:Dopamine / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016962:Sinorhizobium meliloti / Q000737:chemistry*; D014974:Xanthomonas / Q000737:chemistry*; D047391:alpha-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
D002021:Buffers; D005292:Ferricyanides; D037742:Nanotubes, Carbon; D047391:alpha-Cyclodextrins; C098291:alpha-cyclosophorohexadecaose; D047071:beta-Glucans; C028033:potassium ferricyanide; D004298:Dopamine