title: Botryorhodines A-D, antifungal and cytotoxic depsidones from Botryosphaeria rhodina, an endophyte of the medicinal plant Bidens pilosa.
abstract: An endophytic fungus (Botryosphaeria rhodina) was isolated from the stems of the medicinal plant Bidens pilosa (Asteraceae) that is known for its anti-inflammatory, antiseptic and antifungal effects. The ethyl acetate extract of the fungal isolate exhibits significant antifungal activity as well as potent cytotoxic and antiproliferative effects against several cancer cell lines. Activity-guided fractionation resulted in the isolation of a complex of four depsidones, botryorhodines A-D and the auxin indole carboxylic acid. Botryorhodine A and B show moderate to weak cytotoxic activities against HeLa cell lines with a CC(50) of 96.97 microM and 36.41 microM, respectively. In addition, they also show antifungal activity against a range of pathogenic fungi such as Aspergillus terreus (MIC 26.03 microM for botryorhodine A and 49.70 microM for B) and the plant pathogen Fusarium oxysporum (MIC 191.60 microM for botryorhodine A and 238.80 microM for B). A potential role of the endophyte in modulating fungal populations living within or attacking the host plant is discussed.
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745 Jena, Germany.
authors.forename: Randa
authors.initials: R
authors.lastname: Abdou
authors.forename: Kirstin
authors.initials: K
authors.lastname: Scherlach
authors.forename: Hans-Martin
authors.initials: HM
authors.lastname: Dahse
authors.forename: Isabel
authors.initials: I
authors.lastname: Sattler
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001203:Ascomycota / Q000737:chemistry*; D031176:Bidens / Q000382:microbiology*; D045744:Cell Line, Tumor; D053630:Depsides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005658:Fungi / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007210:Indoleacetic Acids / Q000302:isolation & purification; D007783:Lactones / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D018547:Plant Stems / Q000382:microbiology; D010946:Plants, Medicinal
chemical_list: D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D053630:Depsides; D007210:Indoleacetic Acids; D007783:Lactones