title: beta-Actin and beta-Tubulin are components of a heterogeneous mRNA population present in the squid giant axon.
abstract: Previously, we have reported that the squid giant axon contains a heterogeneous population of polyadenylated mRNAs, as well as biologically active polyribosomes. To define the composition of this unique mRNA population, cDNA libraries were constructed to RNA obtained from the axoplasm of the squid giant axon and the parental cell bodies located in the giant fiber lobe. Here, we report that the giant axon contains mRNAs encoding beta-actin and beta-tubulin. The axonal location of these mRNA species was confirmed by in situ hybridization histochemistry, and their presence in the axoplasmic polyribosome fraction was demonstrated by polymerase chain reaction methodology. Taken together, these findings establish the identity of two relatively abundant members of the axonal mRNA population and suggest that key elements of the cytoskeleton are synthesized de novo in the squid giant axon.
authors.affiliation: Molecular Neurobiology and Genetics Program., Western Psychiatric Institute and Clinic, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
authors.forename: B B
authors.initials: BB
authors.lastname: Kaplan
authors.forename: A E
authors.initials: AE
authors.lastname: Gioio
authors.forename: C P
authors.initials: CP
authors.lastname: Capano
authors.forename: M
authors.initials: M
authors.lastname: Crispino
authors.forename: A
authors.initials: A
authors.lastname: Giuditta