Calculation errors of time-varying flux control coefficients obtained from elasticity coefficients by means of summation and connectivity theorems in metabolic control analysis.
This paper investigates the accuracy of a matrix method proposed by other researchers to calculate time-varying flux control coefficients (dynamic FCCs) from elasticity coefficients by means of summation and connectivity theorems in the framework of metabolic control analysis. A mathematical model for the fed-batch penicillin V fermentation process is used as a case example for discussion. Calculated results reveal that this method produces significant calculation errors because the theorems are essentially valid only in steady state, although it may provide rough time-transient behaviors of FCCs. Strictly, therefore, dynamic FCCs should be directly calculated from the differential equations for metabolite concentrations and sensitivities.
Shiraishi
Fumihide
F
Department of Systems Design, Bio-Architecture Center, Kyushu University, Hakozaki, Fukuoka, Japan. fumishira@brs.kyushu-u.ac.jp
Sriyudthsak
Kansuporn
K
Suzuki
Yusuke
Y
D019149:Bioreactors; D005285:Fermentation / Q000502:physiology*; D007700:Kinetics; D008954:Models, Biological; D010404:Penicillin V / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*
D010404:Penicillin V