title: Selective expression of the chemokine receptor XCR1 on cross-presenting dendritic cells determines cooperation with CD8+ T cells.
abstract: The expression of the chemokine receptor XCR1 and the function of its ligand XCL1 (otherwise referred to as ATAC, lymphotactin, or SCM-1) remained elusive to date. In the present report we demonstrated that XCR1 is exclusively expressed on murine CD8(+) dendritic cells (DCs) and showed that XCL1 is a potent and highly specific chemoattractant for this DC subset. CD8(+) T cells abundantly secreted XCL1 8-36 hr after antigen recognition on CD8(+) DCs in vivo, in a period in which stable T cell-DC interactions are known to occur. Functionally, XCL1 increased the pool of antigen-specific CD8(+) T cells and their capacity to secrete IFN-gamma. Absence of XCL1 impaired the development of cytotoxicity to antigens cross-presented by CD8(+) DCs. The XCL1-XCR1 axis thus emerges as an integral component in the development of efficient cytotoxic immunity in vivo.
authors.affiliation: Molecular Immunology, Robert Koch-Institute, 13353 Berlin, Germany.
authors.forename: Brigitte G
authors.initials: BG
authors.lastname: Dorner
authors.forename: Martin B
authors.initials: MB
authors.lastname: Dorner
authors.forename: Xuefei
authors.initials: X
authors.lastname: Zhou
authors.forename: Corinna
authors.initials: C
authors.lastname: Opitz
authors.forename: Ahmed
authors.initials: A
authors.lastname: Mora
authors.forename: Steffen
authors.initials: S
authors.lastname: Güttler
authors.forename: Andreas
authors.initials: A
authors.lastname: Hutloff
authors.forename: Hans W
authors.initials: HW
authors.lastname: Mages
authors.forename: Katja
authors.initials: K
authors.lastname: Ranke
authors.forename: Michael
authors.initials: M
authors.lastname: Schaefer
authors.forename: Robert S
authors.initials: RS
authors.lastname: Jack
authors.forename: Volker
authors.initials: V
authors.lastname: Henn
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kroczek
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D019744:Chemokines, C / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology
chemical_list: D019744:Chemokines, C; D043562:Receptors, G-Protein-Coupled; C091671:XCR1 protein, human; C486249:Xcl1 protein, mouse