title: Effects of N-acetylcysteine and ebselen on arachidonic acid release from astrocytes and neurons cultured in normoxic or simulated ischemic conditions.
abstract: Arachidonic acid (AA) is released from cells after nervous tissue injuries.We treated rat cortical neurons and astrocytes cultured under normoxic or simulated ischemic conditions with N-acetylcysteine (100 or 200 microM) or ebselen (10 or 20 microM). N-acetylcysteine decreased AA release in normoxic astrocytes, while ebselen decreased AA release from astrocytes in both conditions. N-acetylcysteine produced no changes in neuronal AA release. A low dose of ebselen significantly increased AA release from neurons in both conditions. The influence of N-acetylcysteine and ebselen on AA release might be implicated in their effects on astrocytes and neurons, however, the exact mechanisms have yet to be explained.
authors.affiliation: Department of Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pawlas
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Nierwińska
authors.forename: Anna
authors.initials: A
authors.lastname: Stolecka
authors.forename: Bozena
authors.initials: B
authors.lastname: Gabryel
authors.forename: Anna
authors.initials: A
authors.lastname: Pudełko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Małecki
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000378:metabolism*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001393:Azoles / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007511:Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D054833:Isoindoles; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D016718:Arachidonic Acid; C042986:ebselen; D000111:Acetylcysteine