title: Prospective comparative analysis of the angiogenic capacity of monocytes and CD133+ cells in a murine model of hind limb ischemia.
abstract: OBJECTIVE
  The aim of this study was to compare prospectively the vasculogenic capacity of two cell sources, monocytes and CD133+ cells.
  
  
  METHODS
  Cells were obtained from healthy donors by adherence or magnetic selection. Animals studies were performed in a model of hind limb ischemia and different groups were established according to type and number of cells infused. Revascularization was measured by sequential blood flow analysis using a laser Doppler device and by assessing capillary density in the ischemic muscles. In order to locate the infused cells, immunofluorescence and immunocytochemistry techniques were performed and analyzed by light and confocal microscopy.
  
  
  RESULTS
  During the study period there was a significant improvement in both limb perfusion and capillary density in mice treated with either human monocytes or CD133+ cells (P<0.05) compared with non-treated mice. No cells were detected as incorporated into the vessels when 1 x 10(5) cells were used but with higher doses (1 x 10(6)) a few human cells were observed integrated into the vessels in both groups of treated mice. Supernatants of both cell types showed vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and platelet-derived growth factor- AB (PDGF-AB) expression.
  
  
  CONCLUSIONS
  Treatment with human monocytes or CD133+ cells improves blood perfusion and capillary density in a murine model and both cell types seem to stimulate vasculogenesis in a fairly similar way.
authors.affiliation: Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: López-Holgado
authors.forename: Mercedes
authors.initials: M
authors.lastname: Alberca
authors.forename: Fermin Martin
authors.initials: FM
authors.lastname: Sánchez-Guijo
authors.forename: Eva Maria
authors.initials: EM
authors.lastname: Villarón
authors.forename: Jose Vicente
authors.initials: JV
authors.lastname: Rivas
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: López-Novoa
authors.forename: Jesus Garcia
authors.initials: JG
authors.lastname: Briñón
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Arévalo
authors.forename: Enrique
authors.initials: E
authors.lastname: Oterino
authors.forename: Carlos
authors.initials: C
authors.lastname: Santamaría
authors.forename: Jesus Fernando
authors.initials: JF
authors.lastname: San Miguel
authors.forename: Maria Consuelo
authors.initials: MC
authors.lastname: del Cañizo
mesh_terms: D000071916:AC133 Antigen; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002196:Capillaries / Q000473:pathology; D002465:Cell Movement; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000378:metabolism*; D006614:Hindlimb / Q000098:blood supply* / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D007511:Ischemia / Q000473:pathology*; D017078:Laser-Doppler Flowmetry; D051379:Mice; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology*; D009132:Muscles / Q000473:pathology; D018919:Neovascularization, Physiologic; D010455:Peptides / Q000378:metabolism*; D010477:Perfusion; D010641:Phenotype; D012039:Regional Blood Flow; D013194:Staining and Labeling
chemical_list: D000071916:AC133 Antigen; D043925:Angiogenesis Inducing Agents; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse