Rapid detection of aac(6')-Ib-cr quinolone resistance gene by pyrosequencing.
Pyrosequencing was used to rapidly detect aac(6')-Ib and aac(6')-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
Guillard
Thomas
T
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Robert Debré, Rue du Général Koenig, 51092 Reims cedex, France. tguillard@chu-reims.fr
Duval
Véronique
V
Moret
Hélène
H
Brasme
Lucien
L
Vernet-Garnier
Véronique
V
de Champs
Christophe
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D015363:Quinolones / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D017421:Sequence Analysis / Q000379:methods*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones