In vivo assessment of the antimicrobial activity of a calcium-deficient apatite vancomycin drug delivery system in a methicillin-resistant Staphylococcus aureus rabbit osteomyelitis experimental model.
The antimicrobial activities of calcium-deficient apatite loaded with different concentrations (25, 100, and 500 microg/mg) of vancomycin as a filling biomaterial were evaluated in a methicillin-resistant Staphylococcus aureus (MRSA) rabbit acute osteomyelitis model. Bacterial counts in bone, bone marrow, and joint fluid samples treated with forms of the apatite were compared to those in tissue samples receiving a constant intravenous vancomycin infusion after 4 days. This study demonstrates that using a calcium-deficient apatite loaded with vancomycin dramatically decreases the bacterial counts in bone and marrow.
Amador
G
G
CHU de Nantes, 1 Rue Gaston Veil, 44035 Nantes, France.
Gautier
H
H
Le Mabecque
V
V
Miegeville
A F
AF
Potel
G
G
Bouler
J-M
JM
Weiss
P
P
Caillon
J
J
Jacqueline
C
C
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001031:Apatites / Q000737:chemistry*; D001853:Bone Marrow / Q000382:microbiology; D001842:Bone and Bones / Q000382:microbiology; D002118:Calcium / Q000737:chemistry*; D016503:Drug Delivery Systems; D005260:Female; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D010019:Osteomyelitis / Q000188:drug therapy* / Q000382:microbiology; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D014640:Vancomycin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*
D000900:Anti-Bacterial Agents; D001031:Apatites; D014640:Vancomycin; D002118:Calcium