title: Roundup causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus.
abstract: This work aimed to evaluate Roundup effects on biochemical biomarkers of the neotropical fish Prochilodus lineatus. Fish were acutely exposed (6, 24 and 96 h) to 10 mg L(-1) of Roundup (RD) or only water (control) and samples of liver, for antioxidants analysis, and brain and muscle, for acetylcholinesterase (AChE) determination, were collected. Fish exposed to RD for 24h showed reduction on superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and increased glutathione (GSH) content. After 24 and 96h, fish of RD group showed increased glutathione-S-transferase (GST) activity and lipid peroxidation. AChE activity was inhibited in brain after 96h and in muscle after 24 and 96h of exposure. Thus, acute exposure to RD stimulated the biotransformation pathway, with increased GST, but interfered on the antioxidant defenses, with reduction of SOD and GPx activity, leading to the occurrence of lipid peroxidation. Inhibition of AChE showed that RD acts as a contaminant with anti-AChE action.
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina, 86051-990 Londrina, CP 6001 Paraná, Brazil.
authors.forename: Kathya A
authors.initials: KA
authors.lastname: Modesto
authors.forename: Cláudia B R
authors.initials: CB
authors.lastname: Martinez
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D005399:Fishes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D018384:Oxidative Stress; D012623:Seawater / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
chemical_list: D000110:Acetylcholinesterase; D002800:Cholinesterase Inhibitors; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005998:Glycine; D006540:Herbicides; D013482:Superoxide Dismutase; D014874:Water Pollutants, Chemical; D000097797:Glyphosate