Factors affecting the stability of nanoemulsions--use of artificial neural networks.
OBJECTIVE
The aim of this study was to identify the dominant factors affecting the stability of nanoemulsions, using artificial neural networks (ANNs).


METHODS
A nanoemulsion preparation of budesonide containing polysorbate 80, ethanol, medium chain triglycerides and saline solution was designed, and the particle size of samples with various compositions, prepared using different rates and amounts of applied ultrasonic energy, was measured 30 min and 30 days after preparation. Using ANNs, data were modelled and assessed. The derived predictive model was validated statistically and then used to determine the effect of different formulation and processing input variables on particle size growth of the nanoemulsion preparation as an indicator of the preparation stability.


RESULTS
The results indicated that the data can be satisfactorily modelled using ANNs, while showing a high degree of complexity between the dominant factors affecting the stability of the preparation.


CONCLUSIONS
The total amount of applied energy and concentration of ethanol were found to be the dominant factors controlling the particle size growth.
Amani
Amir
A
Institute of Pharmaceutical Innovation, School of Pharmacy, University of Bradford, BD7 1DP, Bradford, UK. aamani@sina.tums.ac.ir
York
Peter
P
Chrystyn
Henry
H
Clark
Brian J
BJ
D019819:Budesonide / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D016571:Neural Networks, Computer; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry; D014465:Ultrasonics
D004655:Emulsions; D011136:Polysorbates; D014280:Triglycerides; D000431:Ethanol; D012965:Sodium Chloride; D019819:Budesonide