title: Novel metal-based pharmacologically dynamic agents of transition metal(II) complexes: designing, synthesis, structural elucidation, DNA binding and photo-induced DNA cleavage activity.
abstract: Novel Schiff base Cu(II), Ni(II), Co(II) and Zn(II) complexes have been designed and synthesized using the macrocyclic ligand derived from the condensation of diethylphthalate with Schiff base, obtained from benzene-1,2-diamine and 3-benzylidene-pentane-2,4-dione. The ligand and its complexes have been characterized by analytical and spectral techniques. DNA binding properties of these complexes have been investigated by UV-vis, viscosity measurements, cyclic voltammetric and differential pulse voltammogram studies. The intrinsic binding constants for Co(II), Ni(II), Cu(II) and Zn(II) complexes are 1.6x10(6), 1.8x10(6), 2.0x10(6) and 1.5x10(6) M(-1) respectively which are obtained from electronic absorption experiment. Control DNA cleavage experiments using pUC19 supercoiled (SC) DNA and minor groove binder (distamycin) suggest the major groove binding tendency for the synthesized complexes. In the presence of a reducing agent like 3-mercaptopropionic acid (MPA), the synthesized complexes show chemical nuclease activity under dark reaction condition. The complexes also show efficient photo-induced DNA cleavage activity on irradiation with a monochromatic UV light of 360 nm in the presence of inhibitors. Control experiments show inhibition of cleavage in the presence of singlet oxygen quencher like sodium azide and enhancement of cleavage in D(2)O, suggesting the formation of singlet oxygen as a reactive species in a type-II process.
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar, India. drn_raman@yahoo.co.in
authors.forename: N
authors.initials: N
authors.lastname: Raman
authors.forename: R
authors.initials: R
authors.lastname: Jeyamurugan
authors.forename: A
authors.initials: A
authors.lastname: Sakthivel
authors.forename: L
authors.initials: L
authors.lastname: Mitu
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D053837:DNA Cleavage; D055664:Electrochemical Techniques; D008024:Ligands; D008027:Light; D008670:Metals / Q000737:chemistry*; D015394:Molecular Structure; D010777:Photochemistry; D013057:Spectrum Analysis / Q000379:methods; D028561:Transition Elements / Q000737:chemistry*; D014783:Viscosity
chemical_list: D008024:Ligands; D008670:Metals; D028561:Transition Elements; D004247:DNA