title: Differential expression of vasoactive mediators in microparticle-challenged lungs of chickens that differ in susceptibility to pulmonary arterial hypertension.
abstract: Pulmonary hypertension syndrome (PHS; ascites) in fast growing meat-type chickens (broilers) is characterized by the onset of idiopathic pulmonary arterial hypertension (IPAH) leading to right-sided congestive heart failure and terminal ascites. Intravenous microparticle (MP) injection is a tool used by poultry geneticists to screen for the broilers that are resistant (RES) or susceptible (SUS) to IPAH in a breeding population. MPs occlude pulmonary arterioles and initiate focal inflammation, causing local tissues and responding leukocytes to release vasoactive mediators such as serotonin (5-HT), endothelin-1 (ET-1), and nitric oxide (NO). RT-PCR was used to examine the differences between RES and SUS broilers in terms of gene expression of ET-1, ET receptor types A and B (ET(A) and ET(B)), the serotonin transporter (SERT), serotonin receptors (5-HT(1A), 5-HT(2A), 5-HT(1B), 5-HT(2B)), endothelial NO synthase (eNOS), and inducible NOS (iNOS) in the lungs of these broilers before (0 h) and after (2, 6, 12, 24, and 48 h) MP injection. In SUS broilers MP injection elicited higher (P < 0.05) pulmonary expression of 5-HT(1A), 5-HT(2B), and ET-1, which promote vasoconstriction and proliferation of pulmonary arterial smooth muscle cells (PASMC). In RES broilers the MP injection elicited higher expression of eNOS, iNOS, and ET(B), which promote vasodilation and inhibit PASMC proliferation. These observations support the hypothesis that the resistance of broiler chickens to IPAH may be due to the higher expression of vasoactive mediators that favor enhanced vasodilation and attenuated vasoconstriction during MP injection challenges to the pulmonary vasculature.
authors.affiliation: Dept. of Poultry Science, Univ. of Arkansas, POSC-420, 1260 W. Maple, Fayetteville, AR 72701, USA. khamal@uark.edu
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Hamal
authors.forename: Robert F
authors.initials: RF
authors.lastname: Wideman
authors.forename: Nicholas B
authors.initials: NB
authors.lastname: Anthony
authors.forename: Gisela F
authors.initials: GF
authors.lastname: Erf
mesh_terms: D000818:Animals; D002645:Chickens; D004198:Disease Susceptibility / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D019332:Endothelin-1 / Q000378:metabolism*; D006976:Hypertension, Pulmonary / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D007275:Injections, Intravenous; D008168:Lung / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008403:Mass Screening / Q000379:methods; D008863:Microspheres; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D011201:Poultry Diseases / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D011651:Pulmonary Artery / Q000503:physiopathology; D017466:Receptors, Endothelin / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D014661:Vasoconstriction / Q000502:physiology; D014664:Vasodilation / Q000502:physiology
chemical_list: D019332:Endothelin-1; D017466:Receptors, Endothelin; D011985:Receptors, Serotonin; D009569:Nitric Oxide; D012701:Serotonin; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III