Sample preparation for plant metabolomics.
Sample preparation in plant metabolomics is a fundamental and critical step with important consequences for the accuracy of results. Depending on the analytical tools and the metabolites of interest, sample preparation has to be decided. However, the various methods reported in the literature have many steps in common and consequently the practical considerations concerning the pros and cons are similar. In this review, each step of the sample preparation - harvesting, drying, extraction and purification - will be discussed in detail.
Kim
Hye Kyong
HK
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, PO Box 9502, 2333 CC Leiden, The Netherlands. h.k.kim@chem.leidenuniv.nl
Verpoorte
Rob
R
D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D055432:Metabolomics; D019906:Nuclear Magnetic Resonance, Biomolecular; D010944:Plants / Q000378:metabolism*; D012997:Solvents
D012997:Solvents