A new sol-gel silica nanovehicle preparation for photodynamic therapy in vitro.
We report a facile silica nanovehicle preparation procedure for hydrophobic drug delivery, which is carried out in water without adding any surfactant and additional catalyst. This strategy includes hydrophobic drug nanopaticle preparation by reprecipitation method and in situ hydrolyzation and polymerization to encapsulate this naoparticle using only N-(beta-amimoethyl)-gamma-aminopropyltriethoxysilane (AETPS). To demonstrate this technique hypocrellin A (HA), a hydrophobic photosensitizing anticancer drug, is embedded into silica nanovehicle using this simple method. The resulting HA encapsulated nanovehicles (HANV) are monodisperse and stable in aqueous solution. Comparative studies with free HA and entrapped HA have demonstrated that the encapsulation effect on the embedded photosensitizer nanoparticle significantly enhances the efficacy of singlet oxygen generation and, thereby, the in vitro photodynamic efficacy.
Zhou
Lin
L
Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing 210097, China.
Liu
Ji-Hua
JH
Zhang
Jian
J
Wei
Shao-Hua
SH
Feng
Yu-Ying
YY
Zhou
Jia-Hong
JH
Yu
Bo-Yang
BY
Shen
Jian
J
D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D011232:Chemical Precipitation; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D005782:Gels; D006367:HeLa Cells; D006801:Humans; D006860:Hydrogen Bonding; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000528:radiation effects; D014677:Pharmaceutical Vehicles / Q000737:chemistry* / Q000633:toxicity; D019800:Phenol; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000528:radiation effects; D011437:Propylamines; D011809:Quinones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry* / Q000633:toxicity; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors
D004337:Drug Carriers; D005782:Gels; D014677:Pharmaceutical Vehicles; D017319:Photosensitizing Agents; D011437:Propylamines; D011809:Quinones; D017382:Reactive Oxygen Species; D012821:Silanes; D026082:Singlet Oxygen; D019800:Phenol; D010569:Perylene; C477625:amino-propyl-triethoxysilane; C048388:hypocrellin A