A simplified paraffin embedding method for small botanical samples.
We present a simplified paraffin embedding method suitable for unsuberized or unlignified small botanical samples (diameter < 0.3 cm). Only 2 h are required to yield plant tissues embedded in paraffin for anatomical observation and molecular analysis. Our method achieved morphological preservation of cell structures and conservation of nucleic acids that were equivalent to the traditional protocol. Fourier transform infrared spectrometry showed that the degree of degradation of the cytoplasmic components (e.g., protein) resulting from our simplified protocol was similar to that of the traditional protocol. The DNA samples embedded using the simplified method was extractable and could be used for PCR analysis. The DNA quality was equivalent to that embedded using the traditional method.
Xing
M
M
College of Life Science, Jilin University, Changchun, China.
Du
Y
Y
Wang
X
X
Niu
L
L
Chen
X
X
D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D035264:Flowers / Q000166:cytology; D018519:Meristem / Q000166:cytology; D016612:Paraffin Embedding / Q000379:methods*; D053205:Pollen Tube / Q000166:cytology; D011506:Proteins / Q000032:analysis / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017550:Spectroscopy, Fourier Transform Infrared; D016707:Tissue Fixation / Q000379:methods*
D018744:DNA, Plant; D011506:Proteins