title: IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana.
abstract: DNA methylation is an epigenetic mark affecting genes and transposons. Screening for mutants that fail to establish DNA methylation yielded two we termed "involved in de novo" (idn) 1 and 2. IDN1 encodes DMS3, an SMC-related protein, and IDN2 encodes a previously unknown double-stranded RNA-binding protein with homology to SGS3. IDN1 and IDN2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Israel
authors.initials: I
authors.lastname: Ausin
authors.forename: Todd C
authors.initials: TC
authors.lastname: Mockler
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
authors.forename: Steven E
authors.initials: SE
authors.lastname: Jacobsen
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000502:physiology*; D019175:DNA Methylation; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; C529025:DMS3 protein, Arabidopsis; C545931:IDN2 protein, Arabidopsis; D016601:RNA-Binding Proteins