Atmospheric pressure chemical ionization and fragmentation of aminomonosaccharides in H2O and D2O.
Aminomonosaccharides (glucosamine, galactosamine, and mannosamine) in H2O and D2O were ionized by atmospheric pressure chemical ionization (APCI) and their fragmentation patterns were investigated to identify them. All the aminomonosaccharides showed the same fragment ions but their relative ion intensities were different. Major product ions generated in H2O were [M + H]+, [M + H - H2O]+, and [2M + H - 3H2O]+, while in D2O were [M(D6) + D]+, [M(D6) + D - D2O]+, and [2M(D6) + D - D2O - 2HDO]+. At a high fragmentor voltage above 120 V, the relative ion intensities of the major product ions showed different trends according to the aminomonosaccharides. For the use of H2O as solvent and eluent, the order of the ion intensity ratio of [M + H - H2O]+/[2M + H - 3H2O]+ was galactosamine > mannosamine > glucosamine. When using D2O as solvent and eluent, the order of the ion intensity ratios of [M(D6) + D - D2O]+/[MD6 + D]+ and [2M(D6) + D - D2O - 2HDO]+/[M(D6) + D]+ was mannosamine > galactosamine > glucosamine. It was found that glucosamine, galactosamine, and mannosamine could be distinguished by the specific trends of the major product ion ratios in H2O and D2O.
Choi
Sung-Seen
SS
Department of Chemistry, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Korea. sschoi@sejong.ac.kr
Kim
Jong-Chul
JC
D000606:Amino Sugars / Q000737:chemistry*; D001274:Atmospheric Pressure; D013058:Mass Spectrometry; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry*
D000606:Amino Sugars; D014867:Water