Efficacy of transnasal nebulized surfactant on experimental otitis media with effusion in guinea pig.
OBJECTIVE
Recently experimental trials have demonstrated that nebulized natural surfactant is effective in the treatment of experimental otitis media with effusion (OME). Two methodologically incomplete studies using animal models of induced OME have reported that nebulized surfactant can improve Eustachian tube function. There have been no reports that investigated the efficacy of the nebulized surfactant for OME using analysis of tympanic membrane vibration. In this study, we evaluated the therapeutic effect of nebulized natural surfactant by investigation of tympanic membrane vibration.


METHODS
The guinea pigs (n=20) were divided into three groups. Four normal guinea pigs without any treatment were used as normal controls. Sixteen pigs were given experimental OME in both ear and then divided into two groups. The OME model of guinea pig was created by transbullar injection 10 microl of lipopolysaccharide in saline. Experimental group 1 (n=8) was nebulized phosphate buffered saline (PBS). Experimental group 2 (n=8) was nebulized natural bovine surfactant. The transnasal nebulization was repeated for 7 days in both experimental groups. On the 8th day, both experimental groups and normal control group were measured the tympanic membrane vibration by laser Doppler vibrometer (LDV), and additionally histopathologic observation was performed by scanning electron microscope (SEM).


RESULTS
The TM vibration velocity in nebulized PBS group showed significantly reduced mainly at low frequencies. However, nebulized natural surfactant group recovered the reduction of tympanic membrane vibration. In nebulized PBS group, SEM showed severe thickened subepithelial layer and hyperplasia of gland structure. However, in nebulized natural surfactant group, SEM showed the reduced the thickness of subepithelial layer and the loss of glandular hyperplasia.


CONCLUSIONS
From our results, the nebulized natural surfactant is postulated to be effective in the treatment of intractable OME in animal model. The measurement of tympanic membrane vibration for analysis of middle ear mechanics was significantly corresponding to the middle ear mucosal changes. LDV is a useful tool for investigate the therapeutic effect of nebulized surfactant in experimental OME. With supporting clinical studies, nebulization of natural surfactant may become noninvasive treatment of OME in future.
Jang
Chul Ho
CH
Department of Otolaryngology, Chonnam National Medical School, Gwangju, South Korea. chulsavio@hanmail.net
Park
Haekyun
H
Choi
Cheol Hee
CH
Cho
Yong Bum
YB
Park
Il Yong
IY
D000281:Administration, Intranasal; D000818:Animals; D004195:Disease Models, Animal; D004432:Ear, Middle; D006168:Guinea Pigs; D006965:Hyperplasia / Q000473:pathology; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects / Q000473:pathology; D009330:Nebulizers and Vaporizers; D010034:Otitis Media with Effusion / Q000188:drug therapy*; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
D013501:Surface-Active Agents