Cdh1-APC is involved in the differentiation of neural stem cells into neurons.
The anaphase-promoting complex (APC) is a multisubunit E3 ubiquitin ligase that controls cell cycle transition in proliferating cells. Recent studies show that Cdh1-APC is active in postmitotic neurons, which regulates axonal growth and patterning, synaptic development and neuronal survival. However, the role of Cdh1-APC in neural stem cells differentiation remains unknown. Using quantitative reverse transcription-PCR, we observed that Cdh1 was expressed higher in neurons than in neural stem cells in vitro. Cdh1 was upregulated, whereas Id2 (one downstream substrate of Cdh1-APC) was downregulated when primary neural stem cells were induced to differentiate into neurons by all-trans retinoic acid. This observation suggests that Cdh1 is involved in the control of neural stem cells differentiated into neurons.
Yao
Wenlong
W
Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Qian
Wei
W
Zhu
Chang
C
Gui
Lingli
L
Qiu
Jin
J
Zhang
Chuanhan
C
D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D000831:Animals, Newborn; D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism*
D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; C577585:Fzr1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D012333:RNA, Messenger; D014212:Tretinoin; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome