title: Tick-borne encephalitis (TBE) virus prevalence and virus genome characterization in field-collected ticks (Ixodes ricinus) from risk, non-risk and former risk areas of TBE, and in ticks removed from humans in Germany.
abstract: Tick-borne encephalitis (TBE) is recognized as the most important viral tick-borne zoonosis in 27 countries in Europe. In this study, ticks were collected in Germany from two non-risk areas in the states of Saxony-Anhalt and Mecklenburg-Western Pomerania, where several single human TBE cases have occurred in recent years. Ticks were also collected from a region in Thuringia, known to be a former risk area for TBE virus (TBEV), where numerous human cases were reported between 1960 and 1975. Detection of TBEV RNA was conducted by real-time RT-PCR. No TBEV was detected in any field-collected ticks. However, ticks were also collected from volunteers living in Bavaria. Three of 239 ticks from this collection were positive for TBEV genome and two genetically distinct TBEV strains were detected and characterized.
authors.affiliation: Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institute, Jena, Germany. christine.klaus@fli.bund.de
authors.forename: C
authors.initials: C
authors.lastname: Klaus
authors.forename: B
authors.initials: B
authors.lastname: Hoffmann
authors.forename: U
authors.initials: U
authors.lastname: Hering
authors.forename: B
authors.initials: B
authors.lastname: Mielke
authors.forename: K
authors.initials: K
authors.lastname: Sachse
authors.forename: M
authors.initials: M
authors.lastname: Beer
authors.forename: J
authors.initials: J
authors.lastname: Süss
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D005858:Germany; D006805:Human Experimentation; D006801:Humans; D018884:Ixodes / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral