title: Fabrication of nano scaled protein monolayer consisting of cytochrome c on self-assembled 11-MUA layer for bioelectronic device.
abstract: The biomolecular/organic hetero-structure films (cytochrome c/11-mercapto-undecanoic acid) on gold substrates were controlled and fabricated with molecular level for developing valuable molecular electronic devices. Cytochrome c is a metalloprotein having redox property, which can be directly applicable to biomemory device as a active element. For efficient immobilization of the protein on the gold substrate, 11-mercapto-undecanoic acid (11-MUA) was used as a linker material between protein and inorganic substrate. The proposed nano scaled biomolecular/organic hetero-structure layer (cytochrome c/11-MUA) on gold surface was investigated by using surface plasmon resonance technique. The molecular morphology of the fabricated protein layer was confirmed by scanning tunneling microscopy. Electrochemical properties of fabricated biomolecular/organic hetero layer were verified using cyclic voltammetry. Their redox properties was sustained over 1000 cycles of cyclic voltametry. It proved that the fabricated film was a suitable platform for the bioelectronic device application.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Taek
authors.initials: T
authors.lastname: Lee
authors.forename: Sang-Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D045304:Cytochromes c / Q000737:chemistry*; D004581:Electronics / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005227:Fatty Acids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D010316:Particle Size; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: C505222:11-mercaptoundecanoic acid; D020099:Coated Materials, Biocompatible; D005227:Fatty Acids; D013438:Sulfhydryl Compounds; D045304:Cytochromes c