title: Synergistic antifibrotic efficacy of statin and protein kinase C inhibitor in hepatic fibrosis.
abstract: Statin has antifibrotic efficacy in human fibrosing diseases, such as pulmonary and renal fibrosis, and is therefore implicated in hepatic fibrosis. However, statin can also activate protein kinase C (PKC), which augments hepatic fibrogenesis and is thereby likely to reduce the antifibrotic efficacy of statin. This study was designed to explore the hypothesis that simultaneous treatment with statin and PKC inhibitor may synergistically enhance antifibrotic efficacy in hepatic fibrosis. Hepatic fibrosis models were established in BALB/c mice by intraperitoneal injection of carbon tetrachloride or thioacetamide for 6 wk. Pravastatin and enzastaurin (PKC inhibitor) were administered by gavage for 5 wk. Cellular apoptosis was explored using 4',6-diamidino-2-phenylindole or terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining and immunoblot analysis. Hepatic fibrosis and hepatic stellate cell (HSC) activation were assessed by morphometric analysis of histological findings and immunohistochemistry for alpha-smooth muscle actin. In vitro, the addition of PKC inhibitor significantly increased statin-induced LX-2 cell apoptosis by enhancing the activation of mitochondrial apoptotic signals. TUNEL-positive HSCs were significantly increased in mice treated with statin + PKC inhibitor compared with those in control or single compound-treated mice. The percentage of area occupied by activated HSCs and the extent of collagen deposition were significantly decreased in mice treated with statin + PKC inhibitor compared with those in control or statin-treated mice. In conclusion, simultaneous treatment with statin and PKC inhibitor synergistically enhanced the antifibrotic efficacy in both in vitro and in vivo models of hepatic fibrosis and may therefore have therapeutic implication for reducing hepatic fibrosis.
authors.affiliation: Prof. of Internal Medicine, Seoul National Univ. Coll. of Medicine, 28 Yungun-dong, Jongno-gu, Seoul 110-744, Korea.
authors.forename: Jong In
authors.initials: JI
authors.lastname: Yang
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Yoon
authors.forename: Yung-Jue
authors.initials: YJ
authors.lastname: Bang
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Soo-Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Byun
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Myung
authors.forename: Won
authors.initials: W
authors.lastname: Kim
authors.forename: Hyo-Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000633:toxicity; D002461:Cell Line, Transformed; D004195:Disease Models, Animal; D004357:Drug Synergism; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D017035:Pravastatin / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007211:Indoles; D047428:Protein Kinase Inhibitors; D013853:Thioacetamide; D002251:Carbon Tetrachloride; D011493:Protein Kinase C; D017035:Pravastatin; C504878:enzastaurin