title: [Comparison of DNA-dependent protein kinase catalytic subunit expression in two lung adenocarcinoma cell lines with different radiosensitivity].
abstract: OBJECTIVE
  To investigate DNA-dependent protein kinase catalytic subunit (DNA-PKcs) content and activity in lung adenocarcinoma cell lines and its correlation with radiosensitivity.
  
  
  METHODS
  The content and activity of DNA-PKcs were analyzed in two lung adenocarcinoma cell lines A549 and H1299 by Western blotting and the Signa TECT DNA-PK assay kit. The dose-survival relationship for two cell lines was analyzed using clonogenic formation assay.
  
  
  RESULTS
  A549 was more radiosensitive than H1299. The survival fractions at 2 Gy (SF2) were 0.7412 in A549 cell line and 0.2473 in H1299 cell line. The content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells (t=10.37, P<0.001). The integrated optical densities were 3.29-/+0.44 in A549 cells and 0.50-/+0.17 in H1299 cells. DNA-PKcs activities in A549 and H1299 cells were 8.29-/+1.37 and 2.47-/+1.09, respectively, showing a significant difference between them (t=5.76, P=0.005).
  
  
  CONCLUSIONS
  DNA-PKcs is an important factor to affect the radiosensitivity of lung adenocarcinoma cell lines.
authors.affiliation: Department of Radiation Oncology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Cen
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.forename: Wei-Xiong
authors.initials: WX
authors.lastname: Li
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D011836:Radiation Tolerance
chemical_list: C109198:CIB1 protein, human; D002135:Calcium-Binding Proteins