title: Double-nucleus enhanced recoupling for efficient 13C MAS NMR correlation spectroscopy of perdeuterated proteins.
abstract: The use of both proton and deuterium dipolar coupling networks to obtain efficient (13)C magic-angle-spinning NMR correlation spectroscopy is introduced. This new strategy aims to improve the efficiency of (13)C spin diffusion in perdeuterated proteins. The method is called double-nucleus enhanced recoupling (DONER), and it provides significantly improved transfer efficiency for carbon spin diffusion at low proton density. The C(alpha) cross-peak intensity in the DONER experiment is approximately 3 and approximately 5 times stronger than those in conventional radio-frequency-assisted diffusion (RAD) and proton-driven spin diffusion (PDSD) experiments, respectively. Remarkably, the full cross-peak pattern for the aliphatic region of an extensively perdeuterated SH3 protein sample can be obtained using the DONER approach with direct (13)C excitation.
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rossle-Str. 10, 13125 Berlin, Germany.
authors.forename: Umit
authors.initials: U
authors.lastname: Akbey
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
authors.forename: Barth-Jan
authors.initials: BJ
authors.lastname: van Rossum
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D003903:Deuterium