title: Changes of substance P during fracture healing in ovariectomized mice.
abstract: Neuropeptides may play an important role in the healing process of osteoporotic fractures. The objective of this study was to determine the role of substance P during osteoporotic fracture healing. One hundred ninety-two mice were randomized into ovariectomy (OVX) and control (CON) group (n=96, respectively). Femoral shaft fracture was created 3 weeks after OVX. Bone mineral density (BMD), micro-CT (microCT) analysis of fracture callus formation and mineralization, microCT analysis of fracture site neovascularization and biomechanical property as well as substance P levels were evaluated 1, 2, 4, and 8 weeks after fracture and compared with CON group. Following OVX-induced bone loss, fracture healing in OVX mice was significantly poorer than that in CON mice, with a significant decrease of substance P at the fracture site at all time points and with the level at early stage (1 and 2 weeks) higher than later stage (4 and 8 weeks). Impaired angiogenesis was also noted in OVX mice. No significant change of substance P level in serum was found between different groups or time points. In conclusion, fracture healing is inferior in OVX-induced bone loss and associated with a significant decrease of substance P. Substance P may play an important role during osteoporotic fracture healing.
authors.affiliation: Department of Orthopaedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Wen-Ge
authors.initials: WG
authors.lastname: Ding
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Zhang
authors.forename: Sheng-Dan
authors.initials: SD
authors.lastname: Jiang
authors.forename: Lei-Sheng
authors.initials: LS
authors.lastname: Jiang
authors.forename: Li-Yang
authors.initials: LY
authors.lastname: Dai
mesh_terms: D000818:Animals; D015519:Bone Density; D002146:Bony Callus / Q000378:metabolism; D005260:Female; D005264:Femoral Fractures / Q000097:blood*; D017102:Fracture Healing; D051379:Mice; D010024:Osteoporosis / Q000097:blood*; D010052:Ovariectomy; D013373:Substance P / Q000097:blood*
chemical_list: D013373:Substance P