title: [Experimental study on combined effect of low temperature and vibration on peripheral circulation function and peripheral nerve function].
abstract: OBJECTIVE
  To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.
  
  
  METHODS
  64 rabbits were divided into control group, low temperature group, vibration group and combined effect group randomly, 16 each group. The changes of concentration of ET, Ang II, NO in plasma and SCV, amplitude of sensory nerve action potential, latency of sensory nerve action potential, MCV, distal amplitude of motor nerve, and distal latency of motor nerve were measured before and after experiment.
  
  
  RESULTS
  After experiment, the concentration of ET, Ang II, NO and SCV, amplitude of sensory nerve action potential, latency of sensory nerve action potential, MCV, distal amplitude of motor nerve, and distal latency of motor nerve were (68.84+/-14.81) pg/ml, (544.01+/-70.20) pg/ml, (123.73+/-9.58) nmol/ml, (25.36+/-6.96) m/s, (1.84+/-0.65) microV, (4.05+/-1.04) m/s, (27.40+/-6.05) m/s, (1.60+/-0.52) microV, (3.51+/-1.30) m/s respectively in low temperature group; (70.22+/-15.02) pg/ml, (540.77+/-68.25) pg/ml, (129.46+/-11.99) nmol/ml, (27.69+/-6.16) m/s, (2.19+/-0.53) microV, (3.86+/-0.89) m/s, (30.03+/-5.21) m/s, (1.65+/-0.49) microV, (3.36+/-l.11) m/s respectively in vibration group; (88.47+/-13.20) pg/ml, (687.38+/-101.44) pg/ml, (70.66+/-4.99) nmol/ml, (20.82+/-3.65) m/s, (1.21+/-0.64) microV, (5.05+/-0.94) m/s, (19.97+/-4.37) m/s, (1.09+/-0.49) microV, (4.49+/-1.26) m/s respectively in combined effect group; compared with pre-experiment, the concentration of ET and Ang II in low temperature group, vibration group and combined effect group were increased after experiment, and the NO was decreased (P<0.05); the nerve conduct velocity and amplitude was decreased and the latency was delayed (P<0.05). After experiment, the concentrations of ET and Ang II in combined effect group were higher than low temperature group and vibration group, and the concentration of NO in combined effect group was lower than low temperature group and vibration group (P<0.05). After experiment, the SCV and MCV in combined effect group were slower than low temperature group and vibration group; the amplitude of sensory nerve action potential and distal amplitude of motor nerve were less than low temperature group and vibration group; the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group. The factorial analysis results indicated the synergistic effect between low temperature and vibration (P<0.05).
  
  
  CONCLUSIONS
  Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.
authors.affiliation: Key Laboratory of Occupational Health and Environmental Medicine, Jining Medical College, Jining 272013, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Chun-zhi
authors.initials: CZ
authors.lastname: Zhang
authors.forename: Yun-mei
authors.initials: YM
authors.lastname: Han
mesh_terms: D000818:Animals; D001775:Blood Circulation / Q000502:physiology*; D003080:Cold Temperature / Q000009:adverse effects*; D005260:Female; D008297:Male; D010525:Peripheral Nerves / Q000503:physiopathology*; D011817:Rabbits; D014732:Vibration / Q000009:adverse effects*