[Influence of electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and non-acupoint on the inflammation and immune reactions in adjuvant arthritis rats].
OBJECTIVE
To observe the effect of electroacupuncture (EA) on the levels of lymphocyte proliferation rate, spleen and serum interleukin-2 (IL-2), serum tumor necrosis factor-alpha (TNF-alpha), so as to analyze its immuno-regulative function in adjuvant arthritis (AA) rats.


METHODS
A total of 40 Wistar rats were randomized into normal control, model, Dazhui (GV 14), Mingmen (GV 4) and non-acupoint groups. Adjuvant arthritis model was established by subcutaneous injection of Freund's complete adjuvant into the paw. EA was applied to "Dazhui" (GV 14), "Mingmen" (GV 4) and non-acupoint (mid-tail) for 20 min, once every other day, 8 times altogether. Paw volume (swelling degree of the voix pedis) was detected; T and B lymphocyte transformation rates (LTR), and IL-2 activity of the spleen tissue were assayed with MTT chromatometry, and serum IL-2 and TNF-alpha contents were determined by radioimmunoassay.


RESULTS
Compared to normal group, the paw volume and serum TNF-alpha content of model group increased significantly (P < 0:01, P < 0.05), and T- and B-lymphocyte proliferation rates and spleen IL-2 activity in model group decreased considerably (P < 0.01). In comparison with model group, paw volumes of GV 14, GV 4, and non-acupoint groups and serum TNF-alpha content of GV 14 group lowered significantly (P < 0.01, P < 0.05); while T- and B-lymphocyte proliferation rates, spleen IL-2 activity in GV 14, GV 4, and non-acupoint groups, as well as serum IL-2 content in GV 14 and GV 4 groups increased remarkably (P < 0.01). Paw volumes of both GV 14 and GV 4 groups were significantly lower than that of non-acupoint group (P < 0.05); T- and B-lymphocyte proliferation rates and IL-2 activity of GV 4 group were significantly higher than those of non-acupoint group (P < 0.05). No significant differences were found between GV 14 and GV 4 groups in paw volume, T- and B-lymphocyte proliferation rates, spleen IL-2 activity, serum IL-2 and TNF-alpha contents, and between GV 14 and non-acupoint groups in T- and B-lymphocyte proliferation rates, as well as among GV 14, GV 4 and non-acupoint groups in serum IL-2 and TNF-alpha contents (P > 0.05).


CONCLUSIONS
Electroacupuncture of GV 14 and GV 4 induced immunoregulation on lymphocyte, IL-2 and TNF-alpha may contribute to its anti-inflammation effect in AA rats.
Li
Hui
H
Department of Acu-moxibustion, Sino-Japan Friendship Hospital, Beijing 100029, China. 2008lihui@163.com
Li
Xiao-Hong
XH
Zhang
Lu-Fen
LF
D015669:Acupuncture Points; D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology* / Q000628:therapy*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D049109:Cell Proliferation; D015671:Electroacupuncture; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000628:therapy; D007376:Interleukin-2 / Q000097:blood / Q000276:immunology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha