title: [Bacterial diversity recovered from Qiyi Glacier and runoff, Qilian Mts].
abstract: Abundance and species diversity of bacteria were investigated respectively by epifluorescence microscope, the culture method and the analysis of 16S rDNA genes, with snow and runoff samples from Qiyi Glacier in the Qilian Mts. According to the results, the total microbial cells and bacterial CFU range from 10(3) to 10(5) cells x mL(-1) and 0-600 cfu x mL(-1), respectively. The 16S rDNA gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: Bacteroidetes, Actinobacteria, Firmicutes and alpha, beta, gamma-Proteobacteria. Based on their 16S rRNA sequences, Bacteroidetes forms the largest cluster in terms of abundance (80% of all isolates) and Pedobacter and Pseudomonas form the dominant genera in terms of abundance (90% of all isolates). Compared with bacteria revealed from ice and snow in Qinghai-Tibet Plateau, South Pole and North Pole which have been reported, the bacteria belonging to Pantoea, Providencia, Terrabacter, Aerococcus and Oxalobacteraceae are especially exist in Qiyi Glacier as far as we know.
authors.affiliation: State Key Laboratory of Cryospheric Science, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China. xiejun@lzb.ac.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.forename: Ning-Lian
authors.initials: NL
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Quan-Lian
authors.initials: QL
authors.lastname: Li
authors.forename: Jian-Qiao
authors.initials: JQ
authors.lastname: He
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Wu
mesh_terms: D001419:Bacteria / Q000145:classification; D041963:Bacteroidetes / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D046448:Ice Cover / Q000382:microbiology*; D011549:Pseudomonas / Q000302:isolation & purification*; D014871:Water Microbiology