Enzyme-mediated protein refolding.
We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.
Okada
Jun
J
Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
Maruyama
Tatsuo
T
Motomura
Konomi
K
Kuroki
Kimiko
K
Maenaka
Katsumi
K
Sakono
Masafumi
M
Goto
Masahiro
M
D004800:Enzymes, Immobilized / Q000378:metabolism; D008722:Methods; D017510:Protein Folding; D020673:Protein Renaturation; D012996:Solutions; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
D004800:Enzymes, Immobilized; D012996:Solutions; D014508:Urea; D014510:Urease