title: Chemical unfolding of enolase from Saccharomyces cerevisiae exhibits a three-state model.
abstract: Enolase is a multifunctional protein that participates in glycolysis and gluconeogenesis and can act as a plasminogen receptor on the cell surface of several organisms, among other functions. Despite its participation in a variety of biological and pathophysiological processes, its stability and folding/unfolding reaction have not been fully explored. In this paper we present, the urea and GdnHCl-induced denaturation of enolase studied by means of fluorescence and circular dichroism spectroscopies. We found that enolase unfolds through a highly reversible pathway, populating a stable intermediate species in a range of experimental conditions. The refolding reaction also exhibits an intermediate state that might have a slightly more compact conformation compared to the unfolding intermediate. The thermodynamic parameters associated with the unfolding reaction are presented and discussed.
authors.affiliation: Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular, Sección de Estudios de Posgrado, ENMyH-Instituto Politécnico Nacional, Guillermo Massieu Helguera No. 239, La Escalera Ticoman, DF, 07320, Mexico.
authors.forename: Dénison S
authors.initials: DS
authors.lastname: Sánchez-Miguel
authors.forename: Jahir
authors.initials: J
authors.lastname: Romero-Jiménez
authors.forename: César A
authors.initials: CA
authors.lastname: Reyes-López
authors.forename: Ana Lilia
authors.initials: AL
authors.lastname: Cabrera-Avila
authors.forename: Normande
authors.initials: N
authors.lastname: Carrillo-Ibarra
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Benítez-Cardoza
mesh_terms: D007700:Kinetics; D008958:Models, Molecular; D010751:Phosphopyruvate Hydratase / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D010751:Phosphopyruvate Hydratase