Antitumor effect of N-succinyl-chitosan nanoparticles on K562 cells.
Polymer nanoparticles have become attractive for its prominent property recent years. In this paper, antitumor effects of N-succinyl-chitosan nanoparticles (NSCNP), a nanoparticles derivated from chitosan, were evaluated in K562 cells, including cell viability comparison, cell morphology analysis, DNA fragmentation detection, cell surface potential and mitochondrial membrane potential (MMP) measurement, intracellular ROS and Ca(2+) concentration evaluation. Our results revealed NSCNP could inhibit the proliferation of K562 with an IC(50) of 14.26 μg/ml (24h); decrease the zeta potential; disrupt the mitochondrial membrane potential; increase ROS generation and Ca(2+) concentration. Cytomorphology study and DNA fragment analysis reveal characteristics of apoptosis and necrosis, indicating that the antitumor effect of NSCNP achieved by necrosis and apoptosis induction in K562 cells.
Luo
Huiying
H
Department of Pharmacology, Gansu College of Traditional Chinese Medicine, No. 35, Dingxi East Road, Lanzhou 730000, PR China. louria@vip.sina.com
Li
Juan
J
Chen
Xuan
X
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009336:Necrosis; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D013499:Surface Properties
D000970:Antineoplastic Agents; C118639:N-succinyl-chitosan; D017382:Reactive Oxygen Species; D048271:Chitosan; D002118:Calcium