title: FEZ1 interacts with CLASP2 and NEK1 through coiled-coil regions and their cellular colocalization suggests centrosomal functions and regulation by PKC.
abstract: FEZ1 was initially described as a neuronal protein that influences axonal development and cell polarization. CLASP2 and NEK1 proteins are present in a centrosomal complex and participate in cell cycle and cell division mechanisms, but their functions were always described individually. Here, we report that NEK1 and CLASP2 colocalize with FEZ1 in a perinuclear region in mammalian cells, and observed that coiled-coil interactions occur between FEZ1/CLASP2 and FEZ1/NEK1 in vitro. These three proteins colocalize and interact with endogenous gamma-tubulin. Furthermore, we found that CLASP2 is phosphorylated and interacts with active PKC isoforms, and that FEZ1/CLASP2 colocalization is inhibited by PMA treatment. Our results provide evidence that these three proteins cooperate in centrosomal functions and open new directions for future studies.
authors.affiliation: Centro de Biologia Molecular Estrutural, Associação Brasileira de Tecnologia de Luz Síncrotron, Rua Giuseppe Máximo Scolfaro, Campinas, SP, Brazil.
authors.forename: Daniel C F
authors.initials: DC
authors.lastname: Lanza
authors.forename: Gabriela V
authors.initials: GV
authors.lastname: Meirelles
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Alborghetti
authors.forename: Camila H
authors.initials: CH
authors.lastname: Abrile
authors.forename: Guido
authors.initials: G
authors.lastname: Lenz
authors.forename: Jörg
authors.initials: J
authors.lastname: Kobarg
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D000072380:NIMA-Related Kinase 1; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C497851:CLASP2 protein, human; D018797:Cell Cycle Proteins; C528597:FEZ1 protein, human; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D014404:Tubulin; C000606914:NEK1 protein, human; D000072380:NIMA-Related Kinase 1; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C