Electrospun silk fibroin scaffolds with macropores for bone regeneration: an in vitro and in vivo study.
We developed three-dimensional electrospun silk fibroin (ESF) scaffolds with controllable pore size. The purpose of this study was to evaluate ESF scaffolds with pores (P-ESF) for bone regeneration via in vitro and in vivo studies, with a comparison to a commercially available porous three-dimensional polylactic acid (PLA) scaffold. P-ESF supported significantly higher proliferation and alkaline phosphatase activity of osteoblasts than PLA in vitro (p < 0.05). Moreover, higher expression levels of activated adhesion-related proteins, including focal adhesion kinase, were observed in the P-ESF than in PLA, as confirmed by western blot analyses. Microcomputed tomography revealed that 78.30% of the original bone volume was attained in the P-ESF implantation group at 7 weeks after critical bone defect formation in rat calvaria. Comparatively, the PLA implantation group showed only 49.31%. Histological evaluation also showed new bone tissue formation upon P-ESF implantation. Taken together, the P-ESF scaffold may be a good bone substitute for bone regeneration.
Park
Sook Young
SY
Department of Dental Anesthesiology and Dental Research Institute, Seoul National University, Seoul, Republic of Korea .
Ki
Chang Seok
CS
Park
Young Hwan
YH
Jung
Hong Moon
HM
Woo
Kyung Mi
KM
Kim
Hyun Jeong
HJ
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D012831:Bombyx; D001861:Bone Regeneration; D005349:Fibroins; D019344:Lactic Acid; D008297:Male; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011108:Polymers; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000000981:diagnostic imaging / Q000502:physiology / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005349:Fibroins