Hands-free sample preparation platform for nucleic acid analysis.
A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.
Baier
T
T
Institut für Mikrotechnik Mainz, Carl-Zeiss-Strasse 18-20, 55129, Mainz, Germany.
Hansen-Hagge
T E
TE
Gransee
R
R
Crombé
A
A
Schmahl
S
S
Paulus
C
C
Drese
K S
KS
Keegan
H
H
Martin
C
C
O'Leary
J J
JJ
Furuberg
L
L
Solli
L
L
Grønn
P
P
Falang
I M
IM
Karlgård
A
A
Gulliksen
A
A
Karlsen
F
F
D045744:Cell Line, Tumor; D004867:Equipment Design; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000191:economics; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014626:Vaginal Smears / Q000191:economics / Q000295:instrumentation*
D012333:RNA, Messenger; D012367:RNA, Viral