Giant unilamellar vesicle electroformation from lipid mixtures to native membranes under physiological conditions.
Giant unilamellar vesicles (GUVs) are well-known model systems, especially because they are easily observable using optical microscopy. In this chapter, we revisit in detail the versatile GUV electroformation protocol. We demonstrate how GUV electroformation can be adapted to various membrane systems including synthetic lipid mixtures, natural lipid extracts, and bilayers containing membrane proteins. Further, we show how to adjust this protocol to a given aqueous environment and prove that GUVs can be obtained under physiologically relevant conditions, that is, in the presence of electrolytes. Finally, we provide firm evidence that electroformation is a method of choice to produce giant vesicles from native cell membranes. This is illustrated with the example of GUV electroformation from red blood cell ghosts in a physiologically pertinent buffer. GUVs obtained in this manner maintain the native membrane asymmetry, thereby validating the physiological relevance of GUV electroformation.
Méléard
Philippe
P
CNRS UMR 6226, ENSCR, Avenue du Général Leclerc, Cedex, France.
Bagatolli
Luis A
LA
Pott
Tanja
T
D002021:Buffers; D004563:Electrochemistry; D004573:Electrolytes / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence
D002021:Buffers; D004573:Electrolytes; D008055:Lipids