Preparing serum for functional complement assays.
To conserve full complement activity, a number of precautions need to be taken in preparing serum. This is particularly the case with mouse complement where the classical pathway is notoriously unstable.
Lachmann
P J
PJ
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK. pjl1000@cam.ac.uk
D000818:Animals; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D003171:Complement Pathway, Classical; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003951:Diagnostic Errors / Q000517:prevention & control; D006287:Health Planning Guidelines; D051379:Mice; D055550:Protein Stability; D015203:Reproducibility of Results; D044967:Serum / Q000378:metabolism; D013048:Specimen Handling
D001798:Blood Proteins; D003165:Complement System Proteins