title: CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: use for quality control.
abstract: Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.
authors.affiliation: CHU Clermont-Ferrand, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France. bevrard@chu-clermontferrand.fr
authors.forename: Bertrand
authors.initials: B
authors.lastname: Evrard
authors.forename: Annie
authors.initials: A
authors.lastname: Dosgilbert
authors.forename: Nathalie
authors.initials: N
authors.lastname: Jacquemot
authors.forename: François
authors.initials: F
authors.lastname: Demeocq
authors.forename: Thibault
authors.initials: T
authors.lastname: Gilles
authors.forename: Jacques
authors.initials: J
authors.lastname: Chassagne
authors.forename: Marc
authors.initials: M
authors.lastname: Berger
authors.forename: Arlette
authors.initials: A
authors.lastname: Tridon
mesh_terms: D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins; D005456:Fluorescent Dyes / Q000032:analysis*; D006084:Graft Rejection / Q000097:blood / Q000628:therapy; D006086:Graft vs Host Disease / Q000097:blood / Q000628:therapy; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D017893:Photopheresis / Q000379:methods* / Q000592:standards; D010835:Phytohemagglutinins / Q000494:pharmacology; D011786:Quality Control; D012494:Sampling Studies; D012751:Sezary Syndrome / Q000097:blood / Q000628:therapy; D012871:Skin Diseases / Q000097:blood / Q000628:therapy; D013388:Succinimides / Q000097:blood*; D013936:Thymidine / Q000097:blood; D014316:Tritium / Q000097:blood
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010835:Phytohemagglutinins; D013388:Succinimides; D014316:Tritium; D013936:Thymidine