Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins.
An enzyme labeled immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins were developed and a comparative evaluation was performed. A polyclonal antibody (BC67) used in both assay formats was raised to saxitoxin-jeffamine-BSA in New Zealand white rabbits. Each assay format was designed as an inhibition assay. Shellfish samples (n=54) were evaluated by each method using two simple rapid extraction procedures and compared to the AOAC high performance liquid chromatography (HPLC) and the mouse bioassay (MBA). The results of each assay format were comparable with the HPLC and MBA methods and demonstrate that an antibody with high sensitivity and broad specificity to PSP toxins can be applied to different immunological techniques. The method of choice will depend on the end-users needs. The reduced manual labor and simplicity of operation of the SPR biosensor compared to ELISA, ease of sample extraction and superior real time semi-quantitative analysis are key features that could make this technology applicable in a high-throughput monitoring unit.
Campbell
Katrina
K
Institute of Agri-Food and Land Use (IAFLU), Queen's University, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. katrina.campbell@qub.ac.uk
Huet
Anne-Catherine
AC
Charlier
Caroline
C
Higgins
Cowan
C
Delahaut
Philippe
P
Elliott
Christopher T
CT
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D057230:Limit of Detection; D051379:Mice; D012530:Saxitoxin / Q000032:analysis* / Q000276:immunology; D012758:Shellfish / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000191:economics / Q000379:methods*
D012530:Saxitoxin