title: An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe.
abstract: Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate that the fission yeast Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or an unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement and breakage at anaphase. This leads to the loss of telomere-proximal markers, though telomere protection and repeat length are unaffected by the absence of Pht1. Strikingly, the chromosome entanglement in pht1Delta anaphase cells can be rescued by forcing chromosome condensation before anaphase onset. We show that the condensin complex, required for the maintenance of anaphase chromosome condensation, prematurely dissociates from chromatin in the absence of Pht1. This and other findings suggest an important role for H2A.Z in the architecture of anaphase chromosomes.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, New York, New York, USA.
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Vincent
authors.initials: V
authors.lastname: Vanoosthuyse
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Fillingham
authors.forename: Assen
authors.initials: A
authors.lastname: Roguev
authors.forename: Stephen
authors.initials: S
authors.lastname: Watt
authors.forename: Thomas
authors.initials: T
authors.lastname: Kislinger
authors.forename: Alex
authors.initials: A
authors.lastname: Treyer
authors.forename: Laura Rocco
authors.initials: LR
authors.lastname: Carpenter
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Bennett
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Hardwick
authors.forename: Nevan J
authors.initials: NJ
authors.lastname: Krogan
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.forename: Michael-Christopher
authors.initials: MC
authors.lastname: Keogh
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D019457:Chromosome Breakage; D015825:Chromosomes, Fungal / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D017353:Gene Deletion; D042822:Genomic Instability; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D012568:Schizosaccharomyces / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D006657:Histones; C091242:PHT1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D000123:Acetyltransferases; D000251:Adenosine Triphosphatases