Ageratum conyzoides essential oil as aflatoxin suppressor of Aspergillus flavus.
Aflatoxin B(1) (AFB(1)) is a highly toxic and carcinogenic metabolite produced by Aspergillus species on food and agricultural commodities. Inhibitory effects of essential oil of Ageratum conyzoides, on the mycelial growth and aflatoxin B(1) production by Aspergillus flavus were studied. Cultures were incubated in yeast extract-sucrose (YES) broth for days at 25 degrees C at the following different concentrations of the essential oil (from 0.0 to 30mug/mL). The essential oil inhibited fungal growth to different extents depending on the concentration, and completely inhibited aflatoxin production at concentrations above 0.10microg/mL. The analysis of the oil by GC/MS showed that its main components are precocene II (46.35%), precocene I (42.78%), cumarine (5.01%) and Trans-caryophyllene (3.02%). Comparison by transmission electron microscopy of the fungal cells, control and those incubated with different concentrations of essential oil, showed ultra-structural changes which were concentration dependent of the essential oil of A. conyzoides. Such ultra-structural changes were more evident in the endomembrane system, affecting mainly the mitochondria. Degradation was also observed in both surrounding fibrils. The ability to inhibit aflatoxin production as a new biological activity of A.conyzoides L. indicates that it may be considered as a useful tool for a better understanding of the complex pathway of aflatoxin biosynthesis.
Nogueira
Juliana H C
JH
Instituto Biológico, Centro de Pesquisa e Desenvolvimento de Sanidade Animal, Brazil.
Gonçalez
Edlayne
E
Galleti
Silvia R
SR
Facanali
Roseane
R
Marques
Márcia O M
MO
Felício
Joana D
JD
D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000633:toxicity; D031163:Ageratum / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D001231:Aspergillus flavus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D046529:Microscopy, Electron, Transmission; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
D000935:Antifungal Agents; D010938:Plant Oils; D016604:Aflatoxin B1