Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome.
BACKGROUND
The profile of intestinal organic acids in irritable bowel syndrome (IBS) and its correlation with gastrointestinal (GI) symptoms are not clear. We hypothesized in this study that altered GI microbiota contribute to IBS symptoms through increased levels of organic acids.


METHODS
Subjects were 26 IBS patients and 26 age- and sex-matched controls. Fecal samples were collected for microbiota analysis using quantitative real-time polymerase chain reaction and culture methods, and the determination of organic acid levels using high-performance liquid chromatography. Abdominal gas was quantified by image analyses of abdominal X-ray films. Subjects completed a questionnaire for GI symptoms, quality of life (QOL) and negative emotion.


RESULTS
Irritable bowel syndrome patients showed significantly higher counts of Veillonella (P = 0.046) and Lactobacillus (P = 0.031) than controls. They also expressed significantly higher levels of acetic acid (P = 0.049), propionic acid (P = 0.025) and total organic acids (P = 0.014) than controls. The quantity of bowel gas was not significantly different between controls and IBS patients. Finally, IBS patients with high acetic acid or propionic acid levels presented with significantly worse GI symptoms, QOL and negative emotions than those with low acetic acid or propionic acid levels or controls.


CONCLUSIONS
These results support the hypothesis that both fecal microbiota and organic acids are altered in IBS patients. A combination of Veillonella and Lactobacillus is known to produce acetic and propionic acid. High levels of acetic and propionic acid may associate with abdominal symptoms, impaired QOL and negative emotions in IBS.
Tana
C
C
Department of Behavioral Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
Umesaki
Y
Y
Imaoka
A
A
Handa
T
T
Kanazawa
M
M
Fukudo
S
S
D019342:Acetic Acid / Q000032:analysis*; D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000737:chemistry* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D043183:Irritable Bowel Syndrome / Q000235:genetics / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D054892:Metagenome; D011422:Propionates / Q000032:analysis*; D011788:Quality of Life; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D011795:Surveys and Questionnaires; D014678:Veillonella / Q000302:isolation & purification
D004269:DNA, Bacterial; D011422:Propionates; C029658:propionic acid; D019342:Acetic Acid