[New derivatives of azobenzene for the directed modification of proteins].
Derivatives of azobenzene which contained a maleimide group in one of the benzene rings (for binding to a protein cysteine residue) and maleimide, hydroxyl, or carboxyl substitutes in another benzene ring were synthesized. The reactivity of these compounds towards a cysteine residue of a protein and their optical properties in a free state and after their attachment to the mutant forms of the SsoII restriction endonuclease were studied.
Khien
Le Tkhi
le T
Chemical Faculty, Faculty of Bioengineering and Bioinformation Science, Belozerskii Institute of Physicochemical Biology, Moscow State University, Moscow, Russia.
Shirling
B
B
Riazanova
A Iu
AIu
Zatsepin
T S
TS
Volkov
E M
EM
Kubareva
E A
EA
Velichko
T I
TI
Pingoud
A
A
Oretskaia
T S
TS
D001391:Azo Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000235:genetics
D001391:Azo Compounds; C045297:CCNGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C009850:azobenzene