PLANdbAffy: probe-level annotation database for Affymetrix expression microarrays.
Standard Affymetrix technology evaluates gene expression by measuring the intensity of mRNA hybridization with a panel of the 25-mer oligonucleotide probes, and summarizing the probe signal intensities by a robust average method. However, in many cases, signal intensity of the probe does not correlate with gene expression. This could be due to the hybridization of the probe to a transcript of another gene, mapping of the probe to an intron, alternative splicing, single nucleotide polymorphisms and other reasons. We have developed a database, PLANdbAffy (available at http://affymetrix2.bioinf.fbb.msu.ru), that contains the results of the alignment of probe sequences from five Affymetrix expression microarrays to the human genome. We have determined the probes matching the transcript-coding regions in the correct orientation. For each such probe alignment region, we determined the mRNA and EST sequences that contain the probe sequence. In the textual part of the database interface we summarize the data on the sequences that cover the probe alignment region and SNPs that are located inside it. The graphical part of our database interface is implemented as custom tracks to the UCSC genome browser that allows one to utilize all the data that are offered by UCSC browser.
Nurtdinov
Ramil N
RN
Departament of Bioengineering and Bioinformatics, MV Lomonosov Moscow State University, Vorbyevy Gory 1-73, Moscow 119992, Russia. ramil@bioinf.fbb.msu.ru
Vasiliev
Mikhail O
MO
Ershova
Anna S
AS
Lossev
Ilia S
IS
Karyagina
Anna S
AS
D017398:Alternative Splicing; D019295:Computational Biology / Q000379:methods* / Q000639:trends; D030541:Databases, Genetic; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D016678:Genome; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods; D020407:Internet; D007438:Introns; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D012984:Software; D014584:User-Computer Interface