title: Optimal parameters of transcorneal electrical stimulation (TES) to be neuroprotective of axotomized RGCs in adult rats.
abstract: We previously showed that transcorneal electrical stimulation (TES) promoted the survival of axotomized retinal ganglion cells (RGCs) of rats. However the relationship between the parameters of TES and the neuroprotective effect of TES on axotomized RGCs was unclear. In the present study, we determined whether the neuroprotective effect of TES is affected by the parameters of TES. Adult male Wistar rats received TES just after transection of the left optic nerve (ON). The pulse duration, current intensity, frequency, waveform, and numbers of sessions of the TES were changed systematically. The alterations of the retina were examined histologically seven days or fourteen days after the ON transection. The optimal neuroprotective parameters were pulse duration of 1 and 2 ms/phase (P < 0.001, each), current intensity of 100 and 200 muA (P < 0.05, each), and stimulation frequency of 1, 5, and 20 Hz (P < 0.001, respectively). More than 30 min of TES was necessary to have a neuroprotective effect (P < 0.001). Symmetric pulses without an inter-pulse interval were most effective (P < 0.001). Repeated TES was more neuroprotective than a single TES at 14 days after ON transection (P < 0.001). Our results indicate that there is a range of optimal neuroprotective parameters of TES for axotomized RGCs of rats. These values will provide a guideline for the use of TES in patients with different retinal and optic nerve diseases.
authors.affiliation: Department of Ophthalmology, Osaka University Graduate School of Medicine, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Morimoto
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Hajime
authors.initials: H
authors.lastname: Sawai
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujikado
mesh_terms: D000818:Animals; D019771:Axotomy; D002452:Cell Count; D002470:Cell Survival / Q000502:physiology; D003315:Cornea / Q000502:physiology; D004558:Electric Stimulation; D004599:Electric Stimulation Therapy / Q000379:methods*; D008297:Male; D009410:Nerve Degeneration / Q000503:physiopathology* / Q000517:prevention & control; D009900:Optic Nerve / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D012162:Retinal Degeneration / Q000503:physiopathology* / Q000517:prevention & control; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013266:Stilbamidines; D013997:Time Factors
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D013266:Stilbamidines