Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay.
We evaluated a multiplexed bead-based assay (xMAP Pneumococcal Immunity assay from Luminex) for the simultaneous determination of antibodies against 14 capsular polysaccharides. Post-vaccination (Pneumovax) antibody concentrations were measured in 35 healthy children, 40 healthy adults, 99 consecutive patients with increased susceptibility to respiratory infection, and 24 patients with a deficient anti-polysaccharide antibody response. The serotype-specific lower 5th percentile (cutoff) value for the post-immunization antibody concentration was determined in healthy individuals. Eleven of 99 patients (11%) failed to mount a response that was >5th percentile of controls for at least 6 of the 14 serotypes tested, whereas only 3 of 75 controls (4%) failed to do so. All patients with known anti-polysaccharide antibody deficiency failed to mount a response that was >5th percentile of controls for at least 6 of the 14 serotypes tested. The XMAP pneumococcal immunity panel appears useful for identifying individuals with a low response to the unconjugated pneumococcal vaccine.
Borgers
Heleen
H
Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Biomedical Sciences Group, Catholic University Leuven, Leuven, Belgium.
Moens
Leen
L
Picard
Capucine
C
Jeurissen
Axel
A
Raes
Marc
M
Sauer
Kate
K
Proesmans
Marijke
M
De Boeck
Kris
K
Casanova
Jean-Laurent
JL
Meyts
Isabelle
I
Bossuyt
Xavier
X
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000276:immunology; D055815:Young Adult
D000907:Antibodies, Bacterial; D022242:Pneumococcal Vaccines