Silver ions increase auxin efflux independently of effects on ethylene response.
Silver nitrate and aminoethoxyvinylglycine (AVG) are often used to inhibit perception and biosynthesis, respectively, of the phytohormone ethylene. In the course of exploring the genetic basis of the extensive interactions between ethylene and auxin, we compared the effects of silver nitrate (AgNO(3)) and AVG on auxin responsiveness. We found that although AgNO(3) dramatically decreased root indole-3-acetic acid (IAA) responsiveness in inhibition of root elongation, promotion of DR5-beta-glucuronidase activity, and reduction of Aux/IAA protein levels, AVG had more mild effects. Moreover, we found that that silver ions, but not AVG, enhanced IAA efflux similarly in root tips of both the wild type and mutants with blocked ethylene responses, indicating that this enhancement was independent of ethylene signaling. Our results suggest that the promotion of IAA efflux by silver ions is independent of the effects of silver ions on ethylene perception. Although the molecular details of this enhancement remain unknown, our finding that silver ions can promote IAA efflux in addition to blocking ethylene signaling suggest that caution is warranted in interpreting studies using AgNO(3) to block ethylene signaling in roots.
Strader
Lucia C
LC
Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA.
Beisner
Erin R
ER
Bartel
Bonnie
B
D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D004347:Drug Interactions / Q000502:physiology; D005030:Ethylenes / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D012835:Silver Nitrate / Q000494:pharmacology*
D005030:Ethylenes; D006131:Growth Inhibitors; D007210:Indoleacetic Acids; C030737:indoleacetic acid; C036216:ethylene; D012835:Silver Nitrate; D005966:Glucuronidase; C088137:aminoethoxyvinylglycine; D005998:Glycine