title: Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals for magnetically triggered delivery of siRNA.
abstract: Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals (PPMCs) were developed for magnetically triggered delivery of siRNA. The positively charged PPMCs formed stable nanosized polyelectrolyte complexes via electrostatic interactions with negatively charged siRNA-polyethylene glycol conjugate (siRNA-s-s-PEG) that was linked via a cleavable disulfide linkage. PPMC/siRNA-s-s-PEG polyelectrolyte complexes were efficiently taken up by cancer cells upon exposure to a magnet, thereby enhancing intracellular uptake and silencing effect of siRNA. The present study suggests that these novel nanomaterials could be potentially utilized for magnetically triggered delivery of various nucleic acid-based therapeutic agents.
authors.affiliation: Department of Biological Sciences and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
authors.forename: Kyuri
authors.initials: K
authors.lastname: Lee
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Bae
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Lee
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Lee
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Ahn
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Park
mesh_terms: D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D019793:Fluorescein; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008280:Magnetics / Q000379:methods*; D018613:Microscopy, Confocal; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D020442:Poloxamer / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D053769:Nanocapsules; D034741:RNA, Small Interfering; D020442:Poloxamer; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D019793:Fluorescein; D052203:Ferrosoferric Oxide