MALDI-TOF mass spectrometry: a powerful tool to study the internalization of cell-penetrating peptides.
This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms.
Aubry
Soline
S
Université Pierre et Marie Curie, 4 place Jussieu, 75005 Paris, France.
Aussedat
Baptiste
B
Delaroche
Diane
D
Jiao
Chen-Yu
CY
Bolbach
Gérard
G
Lavielle
Solange
S
Chassaing
Gérard
G
Sagan
Sandrine
S
Burlina
Fabienne
F
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D057846:Cell-Penetrating Peptides; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D013438:Sulfhydryl Compounds