Electrochemical study of functionalization on the surface of a chitin/platinum-modified glassy carbon paste electrode.
To functionalize chitin surfaces using proteins, we developed a glucose oxidase (GOD)-chitin/platinum-modified glassy carbon paste electrode (GCPE) as a model. In a weakly acidic solution, negatively charged GOD were immobilized by the protonated acetylamide groups on chitin. When the electrode was immersed in a solution containing GOD, the enzyme was readily immobilized due to the electrostatic interaction. In addition, measurements were performed using electrodes made with powders of different sizes because sensor performance depends on the particle sizes of glassy carbon powder.
Sugawara
Kazuharu
K
Faculty of Engineering, Maebashi Institute of Technology, Maebashi, Gunma 371-0816, Japan. kzsuga@maebashi-it.ac.jp
Yugami
Asako
A
Terui
Norifumi
N
Kuramitz
Hideki
H
D000577:Amides / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D002686:Chitin / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D010316:Particle Size; D010984:Platinum / Q000737:chemistry*; D011522:Protons; D013499:Surface Properties
D000577:Amides; D004800:Enzymes, Immobilized; D011522:Protons; D002686:Chitin; D010984:Platinum; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose