title: The Janus-SAM approach for the flexible functionalization of gold and titanium oxide surfaces.
abstract: A novel approach is developed to address the requirement of multiple stamps and inks for microcontact printing (microCP) onto different substrate surfaces. This approach relies on microCP one divalent molecule, which is able to form Janus self-assembled monolayers (JSAMs) with a labile cleavable centre, thus providing a facile method for the chemical derivatization of different substrate surfaces. This study presents an answer to the challenges presented within a highly versatile application, microCP. N-(3-diethylphosphatoxy)propyl-11-mercaptoundecanamide is used for the first time as an ink for microCP onto both gold and titanium oxide surfaces, utilizing the same polydimethylsiloxane stamp. Following printing, the JSAMs are enzymatically treated on these two different substrates to reveal different functional groups. The newly formed surfaces are subjected to additional surface reactions and used for the chemisorption of bovine serum albumin. At each stage, these JSAMs are characterized by X-ray photoelectron spectroscopy and dynamic water-contact-angle measurements. Confocal laser scanning microscopy is used for the characterization of the adsorbed proteins.
authors.affiliation: Institute of Materials Science & Technology (IMT), Friedrich-Schiller-University (FSU), Löbdergraben 32, 07743 Jena, Germany.
authors.forename: Rahila
authors.initials: R
authors.lastname: Bhat
authors.forename: Stephan
authors.initials: S
authors.lastname: Sell
authors.forename: Ralf
authors.initials: R
authors.lastname: Wagner
authors.forename: Jian Tao
authors.initials: JT
authors.lastname: Zhang
authors.forename: Changjiang
authors.initials: C
authors.lastname: Pan
authors.forename: Bora
authors.initials: B
authors.lastname: Garipcan
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Boland
authors.forename: Jörg
authors.initials: J
authors.lastname: Bossert
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Klemm
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Jandt
mesh_terms: D000818:Animals; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006868:Hydrolysis; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D010447:Peptide Hydrolases / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D006046:Gold; D014025:Titanium; D010447:Peptide Hydrolases