title: Deazaflavins as mediators in light-driven cytochrome P450 catalyzed hydroxylations.
abstract: A light-driven deazaflavin-dependent direct enzyme regeneration system has been developed for a P450-BM3 catalyzed CH-activating hydroxylation, thereby avoiding the need for the expensive NADPH cofactor.
authors.affiliation: Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, Mülheim an der Ruhr, Germany.
authors.forename: Felipe E
authors.initials: FE
authors.lastname: Zilly
authors.forename: Andreas
authors.initials: A
authors.lastname: Taglieber
authors.forename: Frank
authors.initials: F
authors.lastname: Schulz
authors.forename: Frank
authors.initials: F
authors.lastname: Hollmann
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology; D001426:Bacterial Proteins; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005415:Flavins / Q000737:chemistry*; D006900:Hydroxylation; D007700:Kinetics; D008027:Light; D009249:NADP; D015202:Protein Engineering / Q000191:economics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D005415:Flavins; D009249:NADP; D003577:Cytochrome P-450 Enzyme System