title: Collagen XVI induces formation of focal contacts on intestinal myofibroblasts isolated from the normal and inflamed intestinal tract.
abstract: In Crohn's disease (CD) the stress-shield of intestinal subepithelial myofibroblasts (ISEMF) provided by intact tissue is disturbed due to inflammation and thus, cells start with remodelling activities. This is characterized by increased numbers of collagen-producing ISEMF causing an uncontrolled, irreversible wound-healing response to the chronic inflammation of the gastrointestinal tract. Reconstitution of the original ECM leads ISEMF to exit this cycle. In contrast, during fibrosis, ISEMF persist. It is known that ISEMF produce and deposit collagen types I, III, IV and V; however synthesis and the role of fibrillar peripheral molecules like collagen type XVI have not been addressed yet. Here, we have analyzed the distribution of collagen XVI in the normal and inflamed bowel wall, its gene and protein expression by ISEMF of different inflammation stages, the cell-matrix interactions in different phases of the inflammatory process and their effect on cell spreading, proliferation and migration. Collagen XVI is deposited in the submucosa of the intestinal wall where it co-localizes with fibrillin-1 and integrin alpha1. ISEMF reveal increasing gene and protein expression of collagen XVI concurrent to increasing inflammation. ISEMF reveal more mature focal adhesion contacts when seeded on collagen XVI resulting in an extensive cell spreading. This involves recruitment of alpha1beta1 integrin, which shows increased cell surface expression on ISEMF in late stages of inflammation. We assume that collagen XVI promotes persistence of ISEMF in the normal and, even stronger in the inflamed bowel wall by stabilizing focal adhesion contacts via cell-matrix interaction preferentially through recruitment of alpha1ss1 integrin into the tips of the focal adhesion contacts.
authors.affiliation: Dept. of Orthopaedic Surgery, Experimental Orthopaedics, University of Regensburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Ratzinger
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Eble
authors.forename: Anja
authors.initials: A
authors.lastname: Pasoldt
authors.forename: Alfred
authors.initials: A
authors.lastname: Opolka
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rogler
authors.forename: Joachim
authors.initials: J
authors.lastname: Grifka
authors.forename: Susanne
authors.initials: S
authors.lastname: Grässel
mesh_terms: D001706:Biopsy; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D003094:Collagen / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D003424:Crohn Disease / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005347:Fibroblasts; D005434:Flow Cytometry; D022001:Focal Adhesions / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C481130:COL16A1 protein, human; D012313:RNA; D003094:Collagen