Hierarchically microporous/macroporous scaffold of magnesium-calcium phosphate for bone tissue regeneration.
Hierarchically 3D microporous/macroporous magnesium-calcium phosphate (micro/ma-MCP) scaffolds containing magnesium ammonium phosphate hexahydrate [NH(4)MgPO(4).6H(2)O] and hydroxyapatite [Ca(10)(PO(4))(6)(OH)(2)] were fabricated from cement utilizing leaching method in the presence of sodium chloride (NaCl) particles and NaCl saturated water solution. NaCl particles produced macroporosity, and NaCl solution acted as both cement liquid and porogens, inducing the formation of microporosity. The micro/ma-MCP scaffolds with porosities varied from 52 to 78% showed well interconnected and open macropores with the sizes of 400-500 microm, and degradation of the scaffolds was significantly enhanced in Tris-HCl solution compared with macroporous MCP (ma-MCP) and corresponding calcium phosphate cement (CPC) scaffolds. Cell attachment and proliferation of MG(63) on micro/ma-MCP were significantly better than ma-MCP and CPC scaffolds because of the presence of microporosity, which enhanced the surface area of the scaffolds. Moreover, the alkaline phosphatase (ALP) activity of the MG(63) cells on micro/ma-MCP was significantly higher than ma-MCP and CPC scaffolds at 7 days, and the MG(63) cells with normal phenotype spread well and formed confluent layers across the macroporous walls of the micro/ma-MCP scaffolds. Histological evaluation confirmed that the micro/ma-MCP scaffolds improved the efficiency of new bone regeneration, and exhibited excellent biocompatibility, biodegradability and faster and more effective osteogenesis in vivo.
Wei
Jie
J
Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
Jia
Junfeng
J
Wu
Fan
F
Wei
Shicheng
S
Zhou
Huanjun
H
Zhang
Hongbo
H
Shin
Jung-Woog
JW
Liu
Changsheng
C
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D048091:Guided Tissue Regeneration / Q000379:methods*; D017616:Magnesium Compounds / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010710:Phosphates / Q000737:chemistry*; D016062:Porosity; D051381:Rats
D018786:Bone Substitutes; D002130:Calcium Phosphates; D017616:Magnesium Compounds; D010710:Phosphates; C030781:magnesium phosphate; C020243:calcium phosphate