title: Evaluation of immunological responses to a glycoprotein G deficient candidate vaccine strain of infectious laryngotracheitis virus.
abstract: Infectious laryngotracheitis virus (ILTV), an alphaherpesvirus, causes severe respiratory disease in poultry. Glycoprotein G (gG) is a virulence factor in ILTV. Recent studies have shown that gG-deficient ILTV is an effective attenuated vaccine however the function of ILTV gG is unknown. This study examined the function and in vivo relevance of ILTV gG. The results showed that ILTV gG binds to chemokines with high affinity and inhibits leukocyte chemotaxis. Specific-pathogen-free (SPF) chickens infected with gG-deficient virus had altered tracheal leukocyte populations and lower serum antibody levels compared with those infected with the parent virus. The findings suggest that the absence of chemokine-binding activity during infection with gG-deficient ILTV results in altered host immune responses.
authors.affiliation: The School of Veterinary Science, The University of Melbourne, Victoria, 3010, Australia. devlinj@unimelb.edu.au
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Devlin
authors.forename: Abel
authors.initials: A
authors.lastname: Viejo-Borbolla
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
authors.forename: Amir H
authors.initials: AH
authors.lastname: Noormohammadi
authors.forename: James R
authors.initials: JR
authors.lastname: Gilkerson
authors.forename: Antonio
authors.initials: A
authors.lastname: Alcami
authors.forename: Carol A
authors.initials: CA
authors.lastname: Hartley
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D006023:Glycoproteins; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D025921:Iltovirus / Q000235:genetics / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D014759:Viral Envelope Proteins; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D037521:Virulence Factors
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D037521:Virulence Factors