Wide-field optical nanoprofilometry using structured illumination.
We combine the differential height measurement concept with structured illumination microscopy to develop wide-field optical nanoprofilometry. Sub-diffraction-limit lateral resolution and axially sectioning imaging are achieved with structured illumination using a liquid-crystal spatial light modulator. As the sample surface is placed into the linear region of the sectioning axial response curve, the signal change owing to topographic variations provides nanometer depth sensitivity. The lateral resolution and the depth profiling accuracy are about 0.3 wavelengths and 6 nm, respectively. Depth profiling on solid-state specimens and label-free superresolution imaging of living cells are demonstrated.
Wang
Chun-Chieh
CC
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
Lee
Kuang-Li
KL
Lee
Chau-Hwang
CH
D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008029:Lighting; D036103:Nanotechnology; D055096:Optical Devices; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D006046:Gold; D012825:Silicon