Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly.
Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II filaments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca(2+).
Smith
Aaron G
AG
Microscopy and Imaging Center, Texas A&M University, College Station, TX 77843-2257, USA.
Johnson
Carol B
CB
Vitha
Stanislav
S
Holzenburg
Andreas
A
D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000378:metabolism*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
D029681:Arabidopsis Proteins; C095113:FTSZ protein, Arabidopsis; C523306:FtsZ1 protein, Arabidopsis; D011994:Recombinant Proteins; D020558:GTP Phosphohydrolases