Lion (Panthera leo) and cheetah (Acinonyx jubatus) IFN-gamma sequences.
Cloning and sequencing of the full length lion and cheetah interferon-gamma (IFN-gamma) transcript will enable the expression of the recombinant cytokine, to be used for production of monoclonal antibodies and to set up lion and cheetah-specific IFN-gamma ELISAs. These are relevant in blood-based diagnosis of bovine tuberculosis, an important threat to lions in the Kruger National Park. Alignment of nucleotide and amino acid sequences of lion and cheetah and that of domestic cats showed homologies of 97-100%.
Maas
Miriam
M
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. M.Maas3@students.uu.nl
Van Rhijn
Ildiko
I
Allsopp
Maria T E P
MT
Rutten
Victor P M G
VP
D002612:Acinonyx / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007371:Interferon-gamma / Q000235:genetics*; D008045:Lions / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013019:South Africa; D013045:Species Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000635:transmission
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007371:Interferon-gamma