Comparison of techniques to evaluate the quantification of Candida spp. in HIV-infected children.
This study sought to compare techniques used to make a quantified evaluation of Candida spp. in children infected with HIV. Twenty-four HIV-infected children (age 3 to 13) were selected. Three sterilized swabs were used for each child: one for the dorsum of the tongue, one for the hard palate mucosa, and one for the right jugal mucosa; each swab was rubbed for 10 seconds and transferred to sterilized test tubes containing 1 mL of 0.9% saline solution. Candida spp. growth was observed in 95.8% of all samples, including 95.7% of tongue samples (Group T), 87.0% of saliva samples, 56.6% of hard palate mucosa samples (Group P), and 47.8% of right jugal mucosa samples (Group J). There was no statistical difference in Candida spp. growth between saliva samples and Group T samples, although both had higher growth compared to Groups P and J (p < 0.05; chi(2)). Regarding the sensitivity of each site for positive Candida spp. growth, Group T samples showed 69.5%, while saliva samples showed 52.2%, Group P samples showed 21.7%, and Group J samples showed 13.04%, with no significant statistical difference between Group T and saliva; however, both were more sensitive than Groups J and P (p < 0.05, chi(2)). It was concluded that whole stimulated saliva and swabbing the tongue were considered satisfactory for measuring Candida spp. in HIV-infected children.
Cerqueira
Daniella Ferraz
DF
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Portela
Maristela Barbosa
MB
Soares
Rosangela Maria De Araujo
RM
De Souza
Ivete Pomarico Ribeiro
IP
Castro
Gloria Fernanda
GF
D000293:Adolescent; D002175:Candida / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000150:complications / Q000382:microbiology*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial / Q000379:methods*; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D012463:Saliva / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014059:Tongue / Q000382:microbiology