Cigarette smoke extract immobilizes human spermatozoa and induces sperm apoptosis.
Cigarette smoking by the male partner adversely affects assisted reproductive techniques, suggesting that it may damage sperm chromatin/DNA and consequently embryo development. The effects of graded concentrations of research cigarettes smoke extract (CSE) on motility, mitochondrial membrane potential (MMP), chromatin integrity and apoptosis were evaluated in spermatozoa obtained from 13 healthy, non-smoking men with normal sperm parameters, by flow cytometry. CSE suppressed sperm motility in a concentration- and time-dependent manner and increased the number of spermatozoa with low MMP, the main source of energy for sperm motility. In addition, CSE had a detrimental effect on sperm chromatin condensation and apoptosis. Indeed, it increased the number of spermatozoa with phosphatidylserine externalization, an early apoptotic sign, and fragmented DNA, a late apoptotic sign, in a concentration- and time-dependent manner. These effects of CSE were of similar or even greater magnitude to those obtained following incubation with tumour necrosis factor-alpha, a cytokine known for its negative impact on sperm function, used as positive control. Since transmission of smoking-induced sperm DNA alterations has been found in pre-implantation embryos, and this may predispose offspring to a greater risk of malformations, cancer and genetic diseases, men seeking to father a child are recommended to give up smoking.
Calogero
Aldo
A
Department of Biomedical Sciences, University of Catania, Catania, Italy. acaloger@unict.it
Polosa
Riccardo
R
Perdichizzi
Anna
A
Guarino
Francesca
F
La Vignera
Sandro
S
Scarfia
Alessia
A
Fratantonio
Enza
E
Condorelli
Rosita
R
Bonanno
Oriana
O
Barone
Nunziata
N
Burrello
Nunziatina
N
D'Agata
Rosario
R
Vicari
Enzo
E
D017209:Apoptosis; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012906:Smoke / Q000009:adverse effects*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology; D014026:Nicotiana / Q000009:adverse effects*
D002843:Chromatin; D012906:Smoke