Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages.
Modification of lipid A is essential for bacterial adaptation to its host. Salmonella Typhimurium LpxR potentially detoxifies lipid A by 3'-O-deacylation; however, the involvement of deacylation in its adaptation remains unclear. LpxR-dependent 3'-O-deacylation was observed in the stationary phase. When macrophages were infected with stationary phase bacteria, the intracellular growth of the lpxR-null strain was lower than that of the wild-type strain. Furthermore, the expression level of inducible nitric oxide synthase was higher in the cells infected with the lpxR-null strain than in the cells infected with the wild-type strain. These results indicate that lipid A 3'-O-deacylation is beneficial for intracellular growth.
Kawano
Miyuki
M
Faculty of Pharmaceutical Sciences, Doshisha Women's College, Kodo, Kyotanabe, Kyoto, Japan.
Manabe
Takayuki
T
Kawasaki
Kiyoshi
K
D000215:Acylation; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D008050:Lipid A / Q000378:metabolism*; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*
D008050:Lipid A; D002265:Carboxylic Ester Hydrolases; C513984:LpxR protein, Salmonella typhimurium