Hepatitis B virus genotypes and evolutionary profiles from blood donors from the northwest region of China.
Hepatitis B virus (HBV) is prevalent in China and screening of blood donors is mandatory. Up to now, ELISA has been universally used by the China blood bank. However, this strategy has sometimes failed due to the high frequency of nucleoside acid mutations. Understanding HBV evolution and strain diversity could help devise a better screening system for blood donors. However, this kind of information in China, especially in the northwest region, is lacking. In the present study, serological markers and the HBV DNA load of 11 samples from blood donor candidates from northwest China were determined. The HBV strains were most clustered into B and C genotypes and could not be clustered into similar types from reference sequences. Subsequent testing showed liver function impairment and increasing virus load in the positive donors. This HBV evolutionary data for China will allow for better ELISA and NAT screening efficiency in the blood bank of China, especially in the northwest region.
Hu
Xing-Bin
XB
Department of Blood Transfusion, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China. hxbyqh@fmmu.edu.cn
Yue
Qiao-Hong
QH
Zhang
Xian-Qing
XQ
Xu
Xue-Qing
XQ
Wen
Yin
Y
Chen
Yao-Zhen
YZ
Cheng
Xiao-Dong
XD
Yang
Liu
L
Mu
Shi-Jie
SJ
D000328:Adult; D001782:Blood Donors; D002681:China; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000097:blood*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012703:Serotyping; D055815:Young Adult