Deazaflavins as mediators in light-driven cytochrome P450 catalyzed hydroxylations.
A light-driven deazaflavin-dependent direct enzyme regeneration system has been developed for a P450-BM3 catalyzed CH-activating hydroxylation, thereby avoiding the need for the expensive NADPH cofactor.
Zilly
Felipe E
FE
Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, Mülheim an der Ruhr, Germany.
Taglieber
Andreas
A
Schulz
Frank
F
Hollmann
Frank
F
Reetz
Manfred T
MT
D001410:Bacillus megaterium / Q000201:enzymology; D001426:Bacterial Proteins; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005415:Flavins / Q000737:chemistry*; D006900:Hydroxylation; D007700:Kinetics; D008027:Light; D009249:NADP; D015202:Protein Engineering / Q000191:economics / Q000379:methods*
D001426:Bacterial Proteins; D005415:Flavins; D009249:NADP; D003577:Cytochrome P-450 Enzyme System