Memory-enhancing corticosterone treatment increases amygdala norepinephrine and Arc protein expression in hippocampal synaptic fractions.
Considerable evidence indicates that glucocorticoid hormones enhance the consolidation of memory for emotionally arousing events through interactions with the noradrenergic system of the basolateral complex of the amygdala (BLA). We previously reported that intra-BLA administration of a beta-adrenoceptor agonist immediately after inhibitory avoidance training enhanced memory consolidation and increased hippocampal expression of the protein product of the immediate early gene activity-regulated cytoskeletal-associated protein (Arc). In the present experiments corticosterone (3 mg/kg, i.p.) was administered to male Sprague-Dawley rats immediately after inhibitory avoidance training to examine effects on long-term memory, amygdala norepinephrine levels, and hippocampal Arc expression. Corticosterone increased amygdala norepinephrine levels 15 min after inhibitory avoidance training, as assessed by in vivo microdialysis, and enhanced memory tested at 48 h. Corticosterone treatment also increased expression of Arc protein in hippocampal synaptic tissue. The elevation in BLA norepinephrine appears to participate in corticosterone-influenced modulation of hippocampal Arc expression as intra-BLA blockade of beta-adrenoceptors with propranolol (0.5 microg/0.2 microL) attenuated the corticosterone-induced synaptic Arc expression in the hippocampus. These findings indicate that noradrenergic activity at BLA beta-adrenoceptors is involved in corticosterone-induced enhancement of memory consolidation and expression of the synaptic-plasticity-related protein Arc in the hippocampus.
McReynolds
Jayme R
JR
School of Behavioral and Brain Sciences, University of Texas at Dallas, Richardson, TX 75080-3021, USA. jrm068000@utdallas.edu
Donowho
Kyle
K
Abdi
Amin
A
McGaugh
James L
JL
Roozendaal
Benno
B
McIntyre
Christa K
CK
D000679:Amygdala / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D003598:Cytoskeletal Proteins / Q000187:drug effects* / Q000235:genetics*; D005938:Glucocorticoids / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D008568:Memory / Q000187:drug effects*; D017551:Microdialysis; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000235:genetics*; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects* / Q000378:metabolism*
D003598:Cytoskeletal Proteins; D005938:Glucocorticoids; D009419:Nerve Tissue Proteins; C092069:activity regulated cytoskeletal-associated protein; D003345:Corticosterone; D009638:Norepinephrine