Detection of polyunsaturated omega-6 fatty acid in human malignant prostate tissue by 1D and 2D high-resolution magic angle spinning NMR spectroscopy.
OBJECTIVE
Polyunsaturated omega-6 fatty acids (PUFAs) have been shown to promote prostate cancer. Here, we describe the use of HRMAS NMR spectroscopy to detect omega-6 PUFA species in prostate tissues.


METHODS
Samples originating from non-malignant (n = 54) and malignant (n = 27) prostate tissues (from 27 prostatectomized men) were studied by 1D (1)H, 2D (1)H-(1)H and (1)H-(13)C HRMAS NMR spectroscopy followed by histopathological characterization.


RESULTS
HRMAS NMR proved to be a powerful, non-destructive method to identify and characterize PUFAs. The omega-6 PUFA was found in 15% of examined human prostate tumors.


CONCLUSIONS
It is possible to detect PUFAs in prostate tissues using our NMR-based spectroscopic approach.
Stenman
Katarina
K
Department of Radiation Sciences, Diagnostic Radiology, Umeå University and University Hospital of Northern Sweden, Umeå, 901 87 Sweden. katarina.stenman@sidb.diagrad.umu.se
Hauksson
Jón B
JB
Gröbner
Gerhard
G
Stattin
Pär
P
Bergh
Anders
A
Riklund
Katrine
K
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D003952:Diagnostic Imaging; D043371:Fatty Acids, Omega-6 / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008495:Medical Oncology / Q000379:methods*; D011467:Prostate / Q000378:metabolism* / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D012680:Sensitivity and Specificity; D013113:Spin Labels
D014408:Biomarkers, Tumor; D043371:Fatty Acids, Omega-6; D013113:Spin Labels