title: Probing the binding of fluoxetine hydrochloride to human serum albumin by multispectroscopic techniques.
abstract: The interaction between human serum albumin (HSA) and fluoxetine hydrochloride (FLX) have been studied by using different spectroscopic techniques viz., fluorescence, UV-vis absorption, circular dichroism and FTIR under simulated physiological conditions. Fluorescence results revealed the presence of static type of quenching mechanism in the binding of FLX to HSA. The values of binding constant, K of FLX-HSA were evaluated at 289, 300 and 310 K and were found to be 1.90x10(3), 1.68x10(3) and 1.45x10(3) M(-1), respectively. The number of binding sites, n was noticed to be almost equal to unity thereby indicating the presence of a single class of binding site for FLX on HSA. Based on the thermodynamic parameters, DeltaH(0) and DeltaS(0) nature of binding forces operating between HSA and FLX were proposed. Spectral results revealed the conformational changes in protein upon interaction. Displacement studies indicated the site I as the main binding site for FLX on HSA. The effect of common ions on the binding of FLX to HSA was also investigated.
authors.affiliation: Department of Chemistry, Karnatak University, Pavate Nagar, Dharwad, Karnataka, India.
authors.forename: Umesha
authors.initials: U
authors.lastname: Katrahalli
authors.forename: Seetharamappa
authors.initials: S
authors.lastname: Jaldappagari
authors.forename: Shankara S
authors.initials: SS
authors.lastname: Kalanur
mesh_terms: D001665:Binding Sites; D005453:Fluorescence; D005473:Fluoxetine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D017367:Selective Serotonin Reuptake Inhibitors / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis / Q000379:methods; D013816:Thermodynamics
chemical_list: D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors; D012709:Serum Albumin