title: Pusillimonas soli sp. nov., isolated from farm soil.
abstract: A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07(T), was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07(T) belongs to the family Alcaligenaceae, class Betaproteobacteria, and is related most closely to Pusillimonas ginsengisoli KCTC 22046(T) (98.6 % sequence similarity) and Pusillimonas noertemannii BN9(T) (96.9 %). The levels of 16S rRNA gene sequence similarity between strain MJ07(T) and members of all other recognized species of the family Alcaligenaceae were below 95.2 %. The G+C content of the genomic DNA of strain MJ07(T) was 59.4 mol%. The detection of a quinone system with ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown aminolipids as major polar lipids and a fatty acid profile with C₁₆:₀ (32.0 %), C₁₇:₀cyclo (24.7 %) and C₁₉:₀cyclo ω8c (11.5 %) as the major components supported the affiliation of strain MJ07(T) to the genus Pusillimonas. Strain MJ07(T) exhibited relatively low levels of DNA-DNA relatedness with respect to P. ginsengisoli KCTC 22046(T) (50 ± 8 %) and P. noertemannii KACC 13183(T) (18 ± 7 %). On the basis of its phenotypic and genotypic properties together with its phylogenetic distinctiveness, strain MJ07(T) (=KCTC 22455(T) =JCM 16386(T)) should be classified in the genus Pusillimonas as the type strain of a novel species, for which the name Pusillimonas soli sp. nov. is proposed.
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
authors.forename: Myungjin
authors.initials: M
authors.lastname: Lee
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
authors.forename: Myoungsoo
authors.initials: M
authors.lastname: Chae
authors.affiliation: Bioenergy Research Center, Korea Institute of Energy Research, 102 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Karea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
mesh_terms: D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S