title: Novel potent apoA-I peptide mimetics that stimulate cholesterol efflux and pre-beta particle formation in vitro.
abstract: Reverse cholesterol transport (RCT) is believed to be the primary mechanism by which HDL and its major protein apoA-I protect against atherosclerosis. Starting from the inactive 22-amino acid peptide representing the consensus sequence of the class A amphipathic helical repeats of apoA-I, we designed novel peptides able to mobilize cholesterol from macrophages in vitro, and to stimulate the formation of 'nascent HDL' particles, with potency comparable to the entire apoA-I protein.
authors.affiliation: IRBM P. Angeletti, Merck Research Laboratories Peptide Centre of Excellence, via Pontina km 30,600, 00040 Pomezia, Italy. raffaele_ingenito@merck.com
authors.forename: Raffaele
authors.initials: R
authors.lastname: Ingenito
authors.forename: Charlotte
authors.initials: C
authors.lastname: Burton
authors.forename: Annunziata
authors.initials: A
authors.lastname: Langella
authors.forename: Xun
authors.initials: X
authors.lastname: Chen
authors.forename: Karolina
authors.initials: K
authors.lastname: Zytko
authors.forename: Antonello
authors.initials: A
authors.lastname: Pessi
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Bianchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D002942:Circular Dichroism; D053442:High-Density Lipoproteins, Pre-beta / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D017510:Protein Folding; D017433:Protein Structure, Secondary
chemical_list: D016632:Apolipoprotein A-I; D053442:High-Density Lipoproteins, Pre-beta; D010455:Peptides; D002784:Cholesterol