Parenteral immunization of fish, Labeo rohita with Poly D, L-lactide-co-glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses.
Immunogenicity of different antigen preparations of outer membrane proteins (OMP) of Aeromonas hydrophila such as Poly d, l-lactide-co-glycolic acid (PLGA) microparticles, oil emulsion, neat OMP and bacterial whole cells were compared through intra-peritoneal injection in fish, Labeo rohita. Among these preparations, PLGA encapsulated antigen stimulated both innate and adaptive immune parameters and the immunogenicity exhibited by PLGA microparticles was significantly higher (p < 0.05) at both 21 and 42 days post-immunization suggesting that the above delivery system would be a novel antigen carrier for parenteral immunization in fish, Labeo rohita.
Behera
T
T
Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-751002, India.
Nanda
P K
PK
Mohanty
C
C
Mohapatra
D
D
Swain
P
P
Das
B K
BK
Routray
P
P
Mishra
B K
BK
Sahoo
S K
SK
D056704:Adaptive Immunity / Q000276:immunology*; D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007114:Immunization / Q000662:veterinary*; D007263:Infusions, Parenteral; D019344:Lactic Acid / Q000737:chemistry / Q000276:immunology*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid