title: A yeast glycolipid biosurfactant, mannosylerythritol lipid, shows potential moisturizing activity toward cultured human skin cells: the recovery effect of MEL-A on the SDS-damaged human skin cells.
abstract: Mannosylerythritol lipids (MELs) are produced in large amounts from renewable vegetable oils by Pseudozyma antarctica, and are the most promising biosurfactants known due to its versatile interfacial and biochemical actions. In order to broaden the application in cosmetics and pharmaceuticals, the skin care property of MEL-A, the major component of MELs, was investigated using a three-dimensional cultured human skin model. The skin cells were cultured and treated with sodium dodecyl sulfate (SDS) solution of 1 wt%, and the effects of different lipids on the SDS-damaged cells were then evaluated on the basis of the cell viability. The viability of the damaged cells was markedly recovered by the addition of MEL-A in a dose-dependent manner. Compared to the control, MEL-A solutions of 5 wt% and 10 wt% gave the recovery rate of 73% and 91%, respectively, while ceramide solution of 1 wt% gave the rate of over 100%. This revealed that MEL-A shows a ceramide-like moisturizing activity toward the skin cells. Considering the drawbacks of natural ceramides, namely limited amount and high production cost, the yeast biosurfactants should have a great potential as a novel moisturizer for treating the damaged skin.
authors.affiliation: Research Institute for Innovations in Sustainable Chemistry, National institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, JAPAN.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Masaru
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Michiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sogabe
authors.forename: Shusaku
authors.initials: S
authors.lastname: Yanagidani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D012867:Skin / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry / Q000633:toxicity*; D013501:Surface-Active Agents / Q000737:chemistry / Q000633:toxicity*; D015003:Yeasts / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D012967:Sodium Dodecyl Sulfate