In vivo tracking of transfused platelets for recovery and survival studies: an appraisal of labeling methods.
The measurement of recovery and survival of platelets is an important decisive factor when 'new' platelet products have been developed. Recovery and survival measurements are mostly performed with radioactive-labeled platelets in healthy volunteers. This approach is required by the FDA for acceptance of platelet products that differ substantially in production or storage conditions from standard methods. However, due to regulatory obstacles, such radiolabeling studies are only carried out in designated institutes. Many countries do not require radioactive labeling studies in volunteers prior to accepting new products, and rather rely on surrogate tests. Also, the European guide to the preparation of blood components does not require this step. This paper reviews alternative, non-radioactive methods, which includes biotinylation of platelets, and discrimination of transfused platelets based on HLA discrepancy. The benefits and disadvantages of these methods will be discussed.
van der Meer
Pieter F
PF
Sanquin Blood Bank South West Region, Plesmanlaan 1a, 2333 BZ Leiden, P.O. Box 2184, 2301 CD Leiden, The Netherlands. p.vandermeer@sanquin.nl
Tomson
Bert
B
Brand
Anneke
A
D000818:Animals; D019921:Biotinylation; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D016922:Cellular Senescence; D002860:Chromium Radioisotopes / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D004285:Dogs; D005456:Fluorescent Dyes; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007205:Indium Radioisotopes / Q000032:analysis; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D010215:Papio; D010976:Platelet Count; D017713:Platelet Transfusion; D013194:Staining and Labeling / Q000379:methods*
D002860:Chromium Radioisotopes; D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes; D006680:HLA Antigens; D007205:Indium Radioisotopes