Contribution of two approaches using electrospray ionization with multi-stage mass spectrometry for the characterization of linseed oil.
A detailed characterization of triacylglycerols (TAGs) present in linseed oil samples from a local producer was performed using electrospray ionization and two mass spectrometric approaches; direct infusion multi-stage mass spectrometry (MS(n)) experiments and liquid chromatography/tandem mass spectrometry (LC/MS/MS) using non-aqueous reversed-phase chromatographic conditions. The combination of both approaches permitted the identification of 26 TAGs. Comparison of the two analytical approaches showed that discrimination of regioisomers was achieved from MS3 data while other isobaric species were separated and identified by LC/MS/MS analysis. The results we obtained were also compared with those previously reported. The TAG composition of the studied linseed oil is qualitatively identical to that of linseed oils from various sources in Europe, Canada, Argentina or India. However, a few differences were observed with regard to the proportions of some TAGs; these can be explained by variations in the culture conditions, climate, and variety of the seeds.
Loutelier-Bourhis
Corinne
C
Université de Rouen, CNRS UMR 6014, COBRA, Rue Tesnière, 76821 Mont Saint Aignan Cedex, France. Corinne.Loutelier@univ-rouen.fr
Zovi
Ornella
O
Lecamp
Laurence
L
Bunel
Claude
C
Lange
Catherine M
CM
D002853:Chromatography, Liquid / Q000379:methods; D008043:Linseed Oil / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry
D014280:Triglycerides; D008043:Linseed Oil