A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase.
A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of [Ru(NH(3))(6)](3+) absorbed on the DNA-AuNPs.
Xu
Xiahong
X
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Nie
Zhou
Z
Chen
Jinhua
J
Fu
Yingchun
Y
Li
Wang
W
Shen
Qinpeng
Q
Yao
Shouzhuo
S
D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry
D055767:Immobilized Proteins; D006046:Gold; D012428:Ruthenium; D004247:DNA; D015040:Zirconium; D011494:Protein Kinases