[Immunochromatographic and latex-agglutination systems for diphtheria toxin detection].
The use of two monoclonal antibody types specific to different epitopes of diphtheria toxin systems have been developed to reveal diphtheria corynebacteria toxigenicity rapidly based on immunochromatographic and latex-agglutination detection of the diphtheria toxin. The methods have been tested on a sample of 36 clinical isolates. The possibility of significant detection of the toxigenic properties of the Corynebacterium strain, grown for 1 day, has been demonstrated. The developed methods allow for the detection of diphtheria toxin in concentrations of 3-4 ng/ml. The developed test systems are a perspective tool for diphtheria diagnostics because of significant time shortening as compared to traditional microbiological methods.
Byzova
N A
NA
Sviridov
V V
VV
Gavrilova
N F
NF
Raspopova
E N
EN
Iakovleva
I V
IV
Generalova
A N
AN
Lukin
Iu V
IuV
Cherkasova
V V
VV
Zherdev
A V
AV
Dzantiev
B B
BB
D000911:Antibodies, Monoclonal / Q000276:immunology; D002845:Chromatography / Q000379:methods; D003353:Corynebacterium diphtheriae / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D004165:Diphtheria / Q000382:microbiology; D004167:Diphtheria Toxin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin