[Atrial myocytes KChIP2 mRNA expression in rheumatic heart disease patients with atrial fibrillation].
OBJECTIVE
To detect the KChIP2 mRNA level in rheumatic heart disease patients with or without atrial fibrillation (AF) by real-time PCR.


METHODS
Right atrial appendage samples from rheumatic heart disease patients with (n = 17) or without AF (n = 13) were obtained during cardiac surgery. Total RNA was extracted from the atrial tissues, and the KChIP2 and Kv4.3 mRNA were detected by SYBR Green I real-time PCR with the GAPDH as the house keeping gene.


RESULTS
The ratio of KChIP2/GAPDH (0.1468 +/- 0.0452 vs. 0.2200 +/- 0.0388, P<0.01) and the ratio of Kv4.3/GAPDH (0.3946 +/- 0.1826 vs. 0.5257 +/- 0.1427, P<0.05) were significantly lower in AF patients compared to non-AF patients.


CONCLUSIONS
Down-regulated atrial KChIP2 and Kv4.3 mRNA expressions in rheumatic heart disease patients with chronic AF might be one of the molecular bases responsible for the down-regulation of the I(to) current density of AF.
Tan
Xiao-qiu
XQ
Department of Myocardium Electrophysiology, Institute of Cardiovasology, Luzhou Medical College, Luzhou 646000, China.
Yang
Yan
Y
Liu
Zhi-fei
ZF
Bai
Zhi-ru
ZR
Zhou
Wen
W
Pei
Jie
J
Chen
Gui-lan
GL
Zeng
Xiao-rong
XR
D000328:Adult; D001281:Atrial Fibrillation / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D051605:Kv Channel-Interacting Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D012214:Rheumatic Heart Disease / Q000235:genetics; D051672:Shal Potassium Channels / Q000235:genetics
C496752:KCNIP2 protein, human; D051605:Kv Channel-Interacting Proteins; D012333:RNA, Messenger; D051672:Shal Potassium Channels