title: Taurine inhibits osteoclastogenesis through the taurine transporter.
abstract: Several studies have suggested a direct link between taurine and bone homeostasis. However, the mechanisms of taurine on the regulation of bone metabolism have not been elucidated. Using a coculture of osteoblasts and bone marrow cells as a model for the study of osteoclastogenesis, RANKL-stimulated RAW264.7 cells and M-CSF- and RANKL-induced bone marrow macrophages were investigated to elucidate the possible roles of taurine in osteoclastogenesis. Taurine inhibited osteoclastogenesis in the coculture of osteoblasts and bone marrow cells, but did not influence the expression of OPG and RANKL in osteoblasts. The taurine transporter (TAUT) expressed by RAW264.7 and bone marrow macrophages exhibited typical taurine uptake activity. Taurine directly reduced osteoclastogenesis in RANKL-stimulated RAW264.7 cells and M-CSF- and RANKL-induced bone marrow macrophages, while TAUT siRNA relieved this effect. Our study demonstrated that taurine directly inhibited osteoclastogenesis through the taurine transporter. Taken together, these data suggest that taurine plays a direct role in bone homeostasis by inhibiting osteoclastogenesis.
authors.affiliation: Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, #86 Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China.
authors.forename: Ling-Qing
authors.initials: LQ
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Cui
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Ji-Cai
authors.initials: JC
authors.lastname: Meng
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Hou-De
authors.initials: HD
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Er-Yuan
authors.initials: EY
authors.lastname: Liao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D018920:Coculture Techniques; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013654:Taurine / Q000494:pharmacology*; D000099247:Taurine Transporters
chemical_list: D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D013654:Taurine; D000099247:Taurine Transporters