Oral immunization of raccoons and skunks with a canine adenovirus recombinant rabies vaccine.
Oral vaccination is an important part of wildlife rabies control programs. Currently, the vaccinia-rabies glycoprotein recombinant virus is the only oral rabies vaccine licensed in the United States, and it is not effective in skunks. In the current study, captive raccoons and skunks were used to evaluate a vaccine developed by incorporating the rabies virus glycoprotein gene into a canine adenovirus serotype 2 vector (CAV2-RVG). Seven of 7 raccoons orally vaccinated with CAV2-RVG developed virus neutralizing antibodies and survived lethal challenge. Five of 5 and 6 of 6 skunks in 2 experimental groups receiving 10-fold different dilutions of CAV2-RVG developed neutralizing antibodies and survived challenge. The results of this preliminary study suggest that CAV2-RVG stimulates protective immunity against rabies in raccoons and skunks.
Henderson
Heather
H
Poxvirus and Rabies Branch, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
Jackson
Felix
F
Bean
Kayla
K
Panasuk
Brian
B
Niezgoda
Michael
M
Slate
Dennis
D
Li
Jianwei
J
Dietzschold
Bernard
B
Mattis
Jeff
J
Rupprecht
Charles E
CE
D006516:Adenoviruses, Canine / Q000276:immunology; D000284:Administration, Oral; D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000032:analysis; D012889:Mephitidae / Q000276:immunology* / Q000821:virology; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011821:Raccoons / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic / Q000276:immunology; D017201:Virus Shedding
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004279:DNA, Viral; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic