Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas.
OBJECTIVE
To investigate the expression of aquaporin (AQP) in eutopic and ectopic endometrial tissues from women with endometriomas.


METHODS
Controlled laboratory research.


METHODS
Hospital-based unit for gynecology and obstetrics and research laboratories.


METHODS
Premenopausal women undergoing laparoscopy for endometriomas.


METHODS
Endometrial biopsy samples obtained from 70 women with endometriomas.


METHODS
Semiquantitative analysis by immunohistochemistry.


RESULTS
Aquaporins 2, 5, and 8 were mainly located in luminal and glandular epithelia. The frequency of positive immunostaining for aquaporins 2, 5, and 8 decreased in ectopic compared with eutopic endometria. Aquaporins 2, 5, and 8 were found at a low frequency in the endometria in early proliferative phases but at a higher frequency in late proliferative and secretory phases. There were no significant differences in the menstrual cycle of the proliferative phase and secretory phase in the two groups.


CONCLUSIONS
Aquaporins 2, 5, and 8 were expressed with greater frequency in eutopic endometrial cells than inectopic endometrial cells, suggesting that eutopic endometrial cells have stronger migration activity than ectopic endometrial cells in women with endometriosis.
Jiang
Xiu-Xiu
XX
Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Wu
Riu-Jin
RJ
Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Xu
Kai-Hong
KH
Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Zhou
Cai-Yun
CY
Department of Pathology, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Guo
Xiao-Yan
XY
Department of Laboratory, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Sun
Ya-Li
YL
Department of Laboratory, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
Lin
Jun
J
Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China. Electronic address: janet418@sina.com.cn.
D000328:Adult; D020346:Aquaporins / Q000378:metabolism*; D002828:Choristoma / Q000378:metabolism* / Q000473:pathology; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D020346:Aquaporins