Remarkable increase in 14C-acetate uptake in an epilepsy model rat brain induced by lithium-pilocarpine.
The present study demonstrates changes in rat brain glial metabolism during the acute phase of epilepsy. Status epilepticus (SE) was induced using the lithium-pilocarpine model. Glial metabolism was measured with (14)C-acetate. Local cerebral blood flow and glucose metabolism were also measured using (14)C-N-isopropyl-p-iodoamphetamine (IMP) and (14)C-2-deoxyglucose (2DG), respectively. At the initiation of the seizure, (14)C-acetate uptake did not change significantly. However, a marked increase was observed 2 h after the pilocarpine injection in all brain regions studied. The increase of brain uptake was transient, and the maximum enhancement was seen at 2 h after the pilocarpine injection. The increase of (14)C-acetate uptake was almost to the same degree in all regions, whereas (14)C-IMP and (14)C-2DG uptakes showed a heterogeneous increase. In the case of (14)C-IMP, the highest increase was observed in the thalamus (280%), and a moderate increase (120 to 150%) was seen in the orbital cortex, cingulate cortex and pyriform cortex. (14)C-2DG uptake increased by 130 to 240% in most regions of the brain, however, an increase of only 40 and 20% was observed in the cerebellum and pons-medulla, respectively. These results demonstrated that glial energy metabolism was markedly enhanced during a prolonged seizure. To our knowledge, this study is the first observation showing large and widespread glial metabolic increases in the rat brain during status epilepticus.
Hosoi
Rie
R
Division of Health Sciences, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. hosoi@sahs.med.osaka-u.ac.jp
Kitano
Daisuke
D
Momosaki
Sotaro
S
Kuse
Kenji
K
Gee
Antony
A
Inoue
Osamu
O
D000085:Acetates / Q000378:metabolism*; D000208:Acute Disease; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D020367:Iofetamine / Q000378:metabolism; D008094:Lithium; D008297:Male; D009457:Neuroglia / Q000378:metabolism*; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D013997:Time Factors
D000085:Acetates; D002250:Carbon Radioisotopes; D010862:Pilocarpine; D008094:Lithium; D003847:Deoxyglucose; D020367:Iofetamine; D005947:Glucose