Potassium-lead-switched G-quadruplexes: a new class of DNA logic gates.
A cation-driven allosteric G-quadruplex DNAzyme (PW17) was utilized to devise a conceptually new class of DNA logic gate based on cation-tuned ligand binding and release. K(+) favors the binding of hemin to parallel-stranded PW17, thereby promoting the DNAzyme activity, whereas Pb(2+) induces PW17 to undergo a parallel-to-antiparallel conformation transition and thus drives hemin to release from the G-quadruplex, deactivating the DNAzyme. Such a K(+)-Pb(2+) switched G-quadruplex, in fact, functions as a two-input INHIBIT logic gate. With the introduction of another input EDTA, this G-quadruplex can be further utilized to construct a reversibly operated IMPLICATION gate.
Li
Tao
T
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China, and Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
Wang
Erkang
E
Dong
Shaojun
S
D000494:Allosteric Regulation; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D007854:Lead / Q000737:chemistry*; D008024:Ligands; D011188:Potassium / Q000737:chemistry*
D021881:DNA, Catalytic; D008024:Ligands; D007854:Lead; D011188:Potassium