Chromatographic estimation of p-coumaric acid and triacontanol in an Ayurvedic root drug patala (Stereospermum suaveolens Roxb.).
A high-performance thin layer chromatographic method has been established for the identification and standardization of Stereospermum suaveolens extracts using two active biomarkers viz. triacontanol and p-coumaric acid in root samples collected from different geographical locations of India. These marker components have different therapeutic activities: p-coumaric acid is a good antioxidant and also has anti-diabetic properties; triacontanol has anti-inflammatory and anti-ulcer properties. Studies on root as well as stem samples showed characteristic bands of triacontanol and p-coumaric acid at R(f) 0.51 and 0.42, respectively. The amount of triacontanol varied from 0.035-8.472 mg/g in the root samples while the amount of p-coumaric acid was in the range 0.081-0.203 mg/g. The limit of detection for triacontanol was 100 ng/spot whereas that for p-coumaric acid was 10 ng/spot.
Srivastava
Neena
N
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India. neenaver@rediffmail.com
Khatoon
Sayadda
S
Rawat
A K S
AK
Rai
Vartika
V
Mehrotra
Shanta
S
D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D005233:Fatty Alcohols / Q000032:analysis*; D008515:Medicine, Ayurvedic; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D011422:Propionates
D003373:Coumaric Acids; D005233:Fatty Alcohols; D010936:Plant Extracts; D011422:Propionates; C047578:1-triacontanol; C495469:p-coumaric acid