NSA2, a novel nucleolus protein regulates cell proliferation and cell cycle.
NSA2 (Nop seven-associated 2) was previously identified in a high throughput screen of novel human genes associated with cell proliferation, and the NSA2 protein is evolutionarily conserved across different species. In this study, we revealed that NSA2 is broadly expressed in human tissues and cultured cell lines, and located in the nucleolus of the cell. Both of the putative nuclear localization signals (NLSs) of NSA2, also overlapped with nucleolar localization signals (NoLSs), are capable of directing nucleolar accumulation. Moreover, over-expression of the NSA2 protein promoted cell growth in different cell lines and regulated the G1/S transition in the cell cycle. SiRNA silencing of the NSA2 transcript attenuated the cell growth and dramatically blocked the cell cycle in G1/S transition. Our results demonstrated that NSA2 is a nucleolar protein involved in cell proliferation and cell cycle regulation.
Zhang
Heyu
H
Department of Immunology, School of Basic Medical Sciences, Peking University, No 38 Xueyuan Road, Beijing 100191, PR China.
Ma
Xi
X
Shi
Taiping
T
Song
Quansheng
Q
Zhao
Hongshan
H
Ma
Dalong
D
D000595:Amino Acid Sequence; D002453:Cell Cycle; D002460:Cell Line; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000378:metabolism*; D049109:Cell Proliferation; D015536:Down-Regulation; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D016601:RNA-Binding Proteins; D016196:S Phase
C547685:NSA2 protein, human; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins