title: [New approaches to the real-time detection of nucleotide mismatches by means of chimeric hybridization probes].
abstract: New approaches to the detection of impaired nucleotides based on the allele specific ligation of a "C probe" followed by rolling circle amplification have been developed. The detection of amplification products was realized by using enzymatic and deoxyribozyme digestion of fluorescently-labeled DNA-RNA-DNA chimeric oligonucleotide structures in cycling probe technology (CPT) in real-time mode.
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, Russia. garafutdinovr@mail.ru
authors.forename: R R
authors.initials: RR
authors.lastname: Garafutdinov
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Nikonorov
authors.forename: D A
authors.initials: DA
authors.lastname: Chemeris
authors.forename: B N
authors.initials: BN
authors.lastname: Postrigan'
authors.forename: O V
authors.initials: OV
authors.lastname: Chubukova
authors.forename: R F
authors.initials: RF
authors.lastname: Talipov
authors.forename: V A
authors.initials: VA
authors.lastname: Vakhitov
authors.forename: A V
authors.initials: AV
authors.lastname: Chemeris
mesh_terms: D020137:Base Pair Mismatch; D005456:Fluorescent Dyes / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes