title: Optimized [1-(13)C]glucose infusion protocol for 13C magnetic resonance spectroscopy at 3T of human brain glucose metabolism under euglycemic and hypoglycemic conditions.
abstract: The effect of insulin-induced hypoglycemia on cerebral glucose metabolism is largely unknown. (13)C MRS is a unique tool to study cerebral glucose metabolism, but the concurrent requirement for [1-(13)C]glucose administration limits its use under hypoglycemic conditions. To facilitate (13)C MRS data analysis we designed separate [1-(13)C]glucose infusion protocols for hyperinsulinemic euglycemic and hypoglycemic clamps in such a way that plasma isotopic enrichment of glucose was stable and comparable under both glycemic conditions. (13)C MR spectra were acquired with optimized (13)C MRS measurement techniques to obtain high quality (13)C MR spectra with these protocols.
authors.affiliation: Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. k.vandeven@rad.umcn.nl
authors.forename: Kim C C
authors.initials: KC
authors.lastname: van de Ven
authors.forename: Marinette
authors.initials: M
authors.lastname: van der Graaf
authors.forename: Cees J J
authors.initials: CJ
authors.lastname: Tack
authors.forename: Dennis W J
authors.initials: DW
authors.lastname: Klomp
authors.forename: Arend
authors.initials: A
authors.lastname: Heerschap
authors.forename: Bastiaan E
authors.initials: BE
authors.lastname: de Galan
mesh_terms: D000328:Adult; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology; D002247:Carbon Isotopes; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007003:Hypoglycemia / Q000378:metabolism* / Q000503:physiopathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009448:Neurochemistry / Q000379:methods*; D055815:Young Adult
chemical_list: D002247:Carbon Isotopes; D005947:Glucose