Association study between C7673T polymorphism in apolipoprotein B gene and cerebral infarction with family history in a Chinese population.
BACKGROUND
Apolipoprotein B (ApoB) levels have been shown to be associate with risk of ischemic stroke. The apolipoprotein B gene (APOB) polymorphisms may influence levels of ApoB and risk of ischemic stroke, but whether they are associated with risk of ischemic cerebral infarction (CI) with family history (CIFH) or not is unknown.


OBJECTIVE
To investigate the possible association of the polymorphism of APOB C7673T with CIFH in Han Chinese from Changsha.


METHODS
The study population included 47 patients with CIFH and 83 patients with cerebral infarction with no family history (CINFH). Control population included 100 healthy subjects with no history of ischemic stroke matched for age, sex, and ethnic background.


METHODS
The APOB C7673T polymorphism was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Plasma lipids levels were also examined by standard enzymatic methods and enzyme-linked immunosorbent assay (ELISA).


METHODS
The statistical analysis was done using SPSS 14.0 software package. A P value < 0.05 was taken as significant.


RESULTS
This study showed an association between the APOB C7673T polymorphism and CI, especially CIFH in Han Chinese from Changsha. The T alleles frequency of the C7673T polymorphism was significantly higher in both the CIFH and CINFH groups when compared with the control group (P < 0.01; P < 0.05; respectively). And the T allele frequency in CIFH group (0.17) was higher than CINFH (0.09) group (P = 0.056). In both the CIFH and CINFH groups the serum levels of TC and LDL cholesterol were significantly high in the TT and TC genotypes than that in the CC genotype (P < 0.01), while the serum level of HDL cholesterol in the TT and TC genotypes was significantly lower than that in the CC genotype (P < 0.05).


CONCLUSIONS
The APOB C7673T polymorphism is related to CI, especially CIFH through changing serum lipid levels in a Chinese population.
Zhang
Le
L
Institute of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan - 410 008, People's Republic of China. macklon12@yahoo.com.cn
Zeng
Yi
Y
Ma
Mingming
M
Yang
Qidong
Q
Hu
Zhongyang
Z
Du
Xiaoping
X
D000328:Adult; D000368:Aged; D001055:Apolipoproteins B / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000097:blood / Q000235:genetics*; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005192:Family Health; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D055106:Genome-Wide Association Study / Q000379:methods; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012189:Retrospective Studies
D001055:Apolipoproteins B; D008055:Lipids