Comparison of quantum dots immunofluorescence histochemistry and conventional immunohistochemistry for the detection of caveolin-1 and PCNA in the lung cancer tissue microarray.
Luminescent semiconductor quantum dots (QDs) are a new class of fluorescent label with wide ranges of applications in cell imaging. In this study, we evaluated the capability of QDs immunofluorescence histochemistry (QDs-IHC) for detecting antigens of caveolin-1 and PCNA in the lung cancer tissue microarray (TMA) in comparison with the conventional immunohistochemistry (IHC) technique. Both methods revealed consistent antigen localization and statistically non-significant detection rates of caveolin-1 and PCNA expressions in our study. However, the sensitivity of QDs-IHC was higher than IHC. The positive detection rates of caveolin-1 and PCNA by QDs-IHC were 57% (40/70) and 86% (60/70), respectively, which were higher than the detection rates of 47% (33/70) and 77% (54/70), respectively, by IHC. Moreover, QDs exhibited a much better photostability, a broader excitation spectrum and a longer fluorescence lifetime. We showed here the advantages of QDs-IHC over IHC for the detection of caveolin-1 and PCNA in lung cancer TMA.
Chen
Honglei
H
Department of Internal Medicine, Renmin Hospital of Wuhan University, 430060, Wuhan, China. chenhongleiwh@126.com
Xue
Jingling
J
Zhang
Yuxia
Y
Zhu
Xiaobo
X
Gao
Jun
J
Yu
Baoping
B
D014408:Biomarkers, Tumor / Q000737:chemistry*; D051242:Caveolin 1 / Q000737:chemistry*; D002681:China; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis / Q000379:methods*
D014408:Biomarkers, Tumor; D051242:Caveolin 1; D018809:Proliferating Cell Nuclear Antigen