Protein synthesis in a synaptosomal fraction from squid brain.
A synaptosomal fraction from squid brain containing a large proportion of well-presarved nerve terminals displays a high rate of [(35)S]methionine incorporation into protein. The reaction is dependent on time and protein concentration, is strongly inhibited by hypo-osmotic shock and cycloheximide, and is not affected by RNase. Chloramphenicol, an inhibitor of mitochondrial protein synthesis, partially inhibits the reaction. The ionic composition of the incubation medium markedly modulates the rate of [(35)S]methionine incorporation. Na(+) and K(+) ions are required for maximal activity, while complete inhibition is achieved by addition of the calcium ionophore A23187 and, to a substantial extent, by tetraethylammonium, ouabain, and high concentrations K(+). A thermostable inhibitor of synaptosomal protein synthesis is also present in the soluble fraction of squid brain. Using sucrose density gradient sedimentation procedures, cytoplasmic polysomes associated with nascent radiolabeled peptide chains have been identified in the synaptosomal preparation. Newly synthesized synaptosomal proteins are largely associated with a readily sedimented particulate fraction and may be resolved by gel electrophoresis into more than 30 discrete bands ranging in size from about 14 to 200 kDa. The electrophoretic pattern of the newly synthesized synaptosomal proteins is significantly different from the corresponding patterns displayed by the giant axon's axoplasm and by glial and nerve cell bodies (in the stellate nerve and ganglion, respectively). On the whole, these observations suggest that the nerve endings from squid brain are capable of protein synthesis.
Crispino
M
M
Dipartimento di Fisiologia Generale e Ambientale, Universitá di Napoli, Naples, Italy; Istituto di Biologia Cellulare, Universitá di Perugia, Perugia, Italy; Istituto Internazionale di Genetica e Biofisica, Naples, Italy; Electron Microscopy Section, University of Ulm, Ulm, Germany; and Department of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh, Pittsburgh, 15213 Pennsylvania.
Castigli
E
E
Perrone Capano
C
C
Martin
R
R
Menichini
E
E
Kaplan
B B
BB
Giuditta
A
A