title: Antioxidant enzyme activity and lipid peroxidation in liver and gill tissues of Nile tilapia (Oreochromis niloticus) following in vivo exposure to domoic acid.
abstract: Domoic acid (DA) is a neurotoxicant produced by Pseudo-nitzschia from diatomeae. Although the neurotoxic and genotoxic effects of DA have been well documented, the number of in vivo studies regarding the oxidative stress inducing effects of DA is quite limited. In this study, in vivo toxic effects of DA were investigated on fish Oreochromis niloticus (Fam: Cichlidae), using oxidative stress biomarkers. Fish were exposed to three different concentrations (1, 5 and 10 microg/g body weight) of DA via intraperitoneal injections and the tissues were sampled at 24, 48 and 72 h post-treatment. Changes in the level of lipid peroxidation, and activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GRd) were evaluated in liver and gill tissues. Our results revealed dose and time dependent increases in the oxidative stress parameters. It was also observed that the toxic effects were more pronounced in liver than in gill tissue.
authors.affiliation: Department of Biology, Faculty of Sciences and Letters, Mersin University, 33343, Ciftlikköy, 33343 Mersin, Turkey. bmazmanci@mersin.edu.tr
authors.forename: Birgül
authors.initials: B
authors.lastname: Mazmanci
authors.forename: Tolga
authors.initials: T
authors.lastname: Cavaş
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D005880:Gills / Q000187:drug effects* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D017210:Tilapia
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; C012301:domoic acid; D007608:Kainic Acid