Quantum dots enhanced ultrasensitive detection of DNA adducts.
Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.
Wang
Zhixin
Z
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, China.
Lu
Meiling
M
Wang
Xiaoli
X
Yin
Ruichuan
R
Song
Yuling
Y
Le
X Chris
XC
Wang
Hailin
H
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D018736:DNA Adducts / Q000032:analysis*; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay; D045663:Quantum Dots; D012680:Sensitivity and Specificity
D018736:DNA Adducts; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide