title: The highly conserved synapsin domain E mediates synapsin dimerization and phospholipid vesicle clustering.
abstract: Synapsins are abundant SV (synaptic vesicle)-associated phosphoproteins that regulate synapse formation and function. The highly conserved C-terminal domain E was shown to contribute to several synapsin functions, ranging from formation of the SV reserve pool to regulation of the kinetics of exocytosis and SV cycling, although the molecular mechanisms underlying these effects are unknown. In the present study, we used a synthetic 25-mer peptide encompassing the most conserved region of domain E (Pep-E) to analyse the role of domain E in regulating the interactions between synapsin I and liposomes mimicking the phospholipid composition of SVs (SV-liposomes) and other pre-synaptic protein partners. In affinity-chromatography and cross-linking assays, Pep-E bound to endogenous and purified exogenous synapsin I and strongly inhibited synapsin dimerization, indicating a role in synapsin oligomerization. Consistently, Pep-E (but not its scrambled version) counteracted the ability of holo-synapsin I to bind and coat phospholipid membranes, as analysed by AFM (atomic force microscopy) topographical scanning, and significantly decreased the clustering of SV-liposomes induced by holo-synapsin I in FRET (Förster resonance energy transfer) assays, suggesting a causal relationship between synapsin oligomerization and vesicle clustering. Either Pep-E or a peptide derived from domain C was necessary and sufficient to inhibit both dimerization and vesicle clustering, indicating the participation of both domains in these activities of synapsin I. The results provide a molecular explanation for the effects of domain E in nerve terminal physiology and suggest that its effects on the size and integrity of SV pools are contributed by the regulation of synapsin dimerization and SV clustering.
authors.affiliation: Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Monaldi
authors.forename: Massimo
authors.initials: M
authors.lastname: Vassalli
authors.forename: Angela
authors.initials: A
authors.lastname: Bachi
authors.forename: Silvia
authors.initials: S
authors.lastname: Giovedì
authors.forename: Enrico
authors.initials: E
authors.lastname: Millo
authors.forename: Flavia
authors.initials: F
authors.lastname: Valtorta
authors.forename: Roberto
authors.initials: R
authors.lastname: Raiteri
authors.forename: Fabio
authors.initials: F
authors.lastname: Benfenati
authors.forename: Anna
authors.initials: A
authors.lastname: Fassio
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D031541:Fluorescence Resonance Energy Transfer; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D018625:Microscopy, Atomic Force; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D055503:Protein Multimerization / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016704:Synapsins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D016704:Synapsins