Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization.
Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.
Morasso
Maria I
MI
Developmental Skin Biology Section, NIH/NIAMS, Bethesda, MD, USA.
D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism*; D005404:Fixatives; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism
D005404:Fixatives; D012333:RNA, Messenger