title: Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity.
abstract: In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, and School of Life Sciences, Sichuan University, Chengdu, 610041, P.R. China.
authors.forename: Chang-Yang
authors.initials: CY
authors.lastname: Gong
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Peng
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Qian
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000633:toxicity; D056724:Immunity, Humoral; D007267:Injections; D007739:L Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D011091:Polyesters / Q000008:administration & dosage / Q000633:toxicity; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000633:toxicity; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011091:Polyesters; D014612:Vaccines; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols