Molecular activation of NF-kappaB, pro-inflammatory mediators, and signal pathways in gamma-irradiated mice.
The effects of gamma-irradiation on inflammatory gene expression, including NF-kappaB activation, in the kidney of C57/BL6 mice exposed to 1-9 Gy doses of (60)Co gamma-irradiation. Radiation enhanced the NF-kappaB activation and oxidative stress caused a dose-dependent disruption in the redox balance. The significance of this study is the new molecular information gained on gamma-irradiation effects through the activation of pro-inflammatory genes by NF-kappaB via the MAPK signaling pathway. Considering the exquisite sensitivity of NF-kappaB and other pro-inflammatory mediators to the redox status, we conclude that the activation of inflammatory processes by irradiation is likely initiated by increased oxidative stress.
Ha
Young Mi
YM
Department of Pharmacy, Pusan National University, Busan, 609-735, Korea.
Chung
Sang Woon
SW
Kim
Ji Min
JM
Kim
Dae Hyun
DH
Kim
Ji Young
JY
Lee
Eun Kyeong
EK
Lee
Jaewon
J
Kim
Young Jin
YJ
Yoo
Mi Ae
MA
Jeong
Kyu Shik
KS
Chung
Hae Young
HY
D000818:Animals; D003037:Cobalt Radioisotopes; D004789:Enzyme Activation / Q000528:radiation effects; D005720:Gamma Rays; D019803:Glutathione Disulfide / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects*; D015854:Up-Regulation / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000379:methods*
D003037:Cobalt Radioisotopes; D018836:Inflammation Mediators; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D020928:Mitogen-Activated Protein Kinases; D019803:Glutathione Disulfide