title: Binding kinetics of bisintercalator Triostin a with optical tweezers force mechanics.
abstract: The binding kinetics of the intercalative binding of Triostin A to lambda-DNA was investigated by measuring the force extension response of the DNA-ligand complexes with an optical tweezers system. These force response curves, containing the information about different binding properties, were analyzed based on a recent method (put forth by another research group) for monointercalators that was extended to bisintercalators. Our binding analysis reveals an exponential dependence of the association constant on the applied external force as well as a decreasing binding site size. In general, our results are in agreement with those for the monointercalator ethidium. However, to explain the high-force binding site size, a new model for bisintercalation of Triostin A at high forces is proposed.
authors.affiliation: Department of Physics, Experimental Biophysics and Applied Nanoscience, Bielefeld University, Bielefeld, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kleimann
authors.forename: Andy
authors.initials: A
authors.lastname: Sischka
authors.forename: Andre
authors.initials: A
authors.lastname: Spiering
authors.forename: Katja
authors.initials: K
authors.lastname: Tönsing
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
authors.forename: Ulf
authors.initials: U
authors.lastname: Diederichsen
authors.forename: Dario
authors.initials: D
authors.lastname: Anselmetti
mesh_terms: D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D007700:Kinetics; D052898:Optical Tweezers; D011810:Quinoxalines / Q000737:chemistry; D013314:Stress, Mechanical
chemical_list: D000900:Anti-Bacterial Agents; D011810:Quinoxalines; C031945:triostin A; D004247:DNA; D004996:Ethidium