Antimicrobial and cytotoxic constituents from leaves of Sapium baccatum.
Six compounds, namely, Lupeol (1), Betulin (2), beta-Taraxerol (3), Taraxerone (4), Stigmasterol (5) and beta-Sitosterol (6) were isolated from the petroleum ether extract of the leaves of Sapium baccatum based on spectroscopic evidence. Lupeol (1), Betulin (2) and Stigmasterol (5) were isolated for the first time from this plant. The cytotoxic potential of the different solvent extracts (methanol, petroleum ether, carbon-tetrachloride and dichloromethane); six column fractions (F-4, F-7, F-10, F-12, F-18 and F-22) of petroleum ether extract and three pure compounds 1, 4 and 6 were determined by using brine shrimp lethality bioassay. The LC50 of all the tested samples were showed to be lethal to brine shrimp nauplii. However, petroleum ether, carbon-tetrachloride extract, column fractions F-4 and F-18 of petroleum ether extract and pure compound 6 showed quite potent activity in brine shrimp lethality bioassay with LC50 1.33, 1.35, 1.40, 1.58 and 1.58 microg/ml, respectively. These result suggested that they might be contain antitumor or pesticidal activity. Further, the methanol extract and four column fractions (F-7, F-12, F-18 and F-22) of petroleum ether showed significant activity against the tested microorganisms.
Ahmed
Yunus
Y
Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia 7003, Bangladesh.
Sohrab
Md Hossain
MH
Al-Reza
Sharif M
SM
Tareq
Faqir Shahidulla
FS
Hasan
Choudhury M
CM
Sattar
M A
MA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects; D005591:Chemical Fractionation; D003603:Cytotoxins / Q000494:pharmacology*; D008136:Longevity / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D031287:Sapium / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D023382:Toxicity Tests, Acute
D000900:Anti-Bacterial Agents; D003603:Cytotoxins; D010936:Plant Extracts; D012997:Solvents