title: Crystallization and preliminary X-ray analysis of the stress-response PPM phosphatase RsbX from Bacillus subtilis.
abstract: RsbX from Bacillus subtilis is a manganese-dependent PPM phosphatase and negatively regulates the signal transduction of the general stress response by the dephosphorylation of RsbS and RsbR, which are activators of the alternative RNA polymerase sigma factor SigB. In order to elucidate the structural-functional relationship of its Ser/Thr protein-phosphorylation mechanism, an X-ray crystallographic diffraction study of RsbX was performed. Recombinant RsbX was expressed in Escherichia coli, purified and crystallized. Crystals were obtained using the sitting-drop vapour-diffusion method and X-ray diffraction data were collected to 1.06 angstrom resolution with an R(merge) of 8.1%. The crystals belonged to the triclinic space group P1, with unit-cell parameters a = 33.3, b = 41.7, c = 68.6 angstrom , alpha = 98.8, beta = 90.0, gamma = 108.4 degrees.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Suganuma
authors.forename: Aik Hong
authors.initials: AH
authors.lastname: Teh
authors.forename: Masatomo
authors.initials: M
authors.lastname: Makino
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Shimizu
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kaneko
authors.forename: Kunio
authors.initials: K
authors.lastname: Hirata
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D010749:Phosphoprotein Phosphatases