title: Collagen type IV and Perlecan exhibit dynamic localization in the Allantoic Core Domain, a putative stem cell niche in the murine allantois.
abstract: A body of evidence suggests that the murine allantois contains a stem cell niche, the Allantoic Core Domain (ACD), that may contribute to a variety of allantoic and embryonic cell types. Given that extracellular matrix (ECM) regulates cell fate and function in niches, the allantois was systematically examined for Collagen type IV (ColIV) and Perlecan, both of which are associated with stem cell proliferation and differentiation. Not only was localization of ColIV and Perlecan more widespread during gastrulation than previously reported, but protein localization profiles were particularly robust and dynamic within the allantois and associated visceral endoderm as the ACD formed and matured. We propose that these data provide further evidence that the ACD is a stem cell niche whose activity is synchronized with associated visceral endoderm, possibly via ECM proteins.
authors.affiliation: Department of Anatomy, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin 53706, USA.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Mikedis
authors.forename: Karen M
authors.initials: KM
authors.lastname: Downs
mesh_terms: D000482:Allantois / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D024141:Collagen Type IV / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development / Q000502:physiology; D005321:Extraembryonic Membranes / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D011247:Pregnancy; D055153:Stem Cell Niche / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D024141:Collagen Type IV; D019812:Heparan Sulfate Proteoglycans; C071927:perlecan