Fabrication of protein chip by magnetic force.
This research describes a new immobilizing method of many kinds of biomaterials (enzyme, antibody, and DNA) on a transducer array using magnetic force interaction as the short-range force. The method composes two immobilizing steps. In the first step, same biomaterials are immobilized on metal particles. In the second step, the particles are arranged by the fluidic self-assembly method at random on an array. An array immobilized many kinds of the particles become multichannel biosensor. The biosensor can apply to DNA chip, protein chip, multienzyme electrode, and so on. The metal particles and the array were fabricated by micromachining manufacture. The metal particles were multilayer structure (gold, titanium, and nickel). In the array case, sidewalls of patterning nickel dots on an array were covered by thick negative photoresist (SU-8), and the array was magnetized. The array and the particles were mixed in buffer solution, and were arranged by magnetic force interaction. A quarter of total nickel dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost with gold surface on top. The immobilization of the biomaterials to metal particles was able to materialize it by using 3-CPD. This confirmed an activity by the luminol radiation.
Choi
Yong-Sung
YS
Department of Electrical Engineering, Dongshin University, 252 Daeho-Dong, Naju, Jeonnam 520-714, Korea.
Jang
Gil-Soo
GS
Hong
Kyung-Jin
KJ
Lee
Kyung-Sup
KS
D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008280:Magnetics; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects*
D011506:Proteins