A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies.
OBJECTIVE
Volume reduction is the usual process in cord blood banking that has some advantages regarding reducing the storage space and dimethyl sulfoxide (DMSO) quantity in the final product. The volume reduction methodology must guarantee high cell recovery and red blood cell (RBC) depletion by reducing all the umbilical cord blood (UCB) units to a standard volume.


METHODS
We analyzed and compared critically three different volume reduction methods [hydroxyethylstarch (HES), top and bottom with Optipress II and Compomat G4, and AXP] used at the Valencia Cord Blood Bank over 10 years.


RESULTS
The highest significant RBC depletion was achieved with the AXP system (P<0.001), while the top and bottom system with Compomat G4 and an adjusted buffy coat (BC) volume to 41 mL enabled the best total nucleated cell (TNC) recovery (P<0.001). TNC recovery and RBC depletion were similar for AXP and HES with an adjusted volume to 21 mL. In the multivariate analysis, when analyzing all cases, the BC volume set significantly influenced TNC, CD34+ and lymphocyte recoveries and RBC depletion (P<0.001). RBC depletion was significantly influenced by the initial volume and initial RBC content of UCB units (P<0.001).


CONCLUSIONS
AXP is a highly efficient method for RBC depletion, providing the same TNC recovery as HES method with a final volume of 41 mL. AXP has the advantages of being an automatic and functionally closed system that shortens and better standardizes the proceedings. Top and bottom is a closed system that allows better TNC recoveries when the BC volume set is 41 mL.
Solves
Pilar
P
Cord Blood Bank Transfusion Center, Valencia, Spain.
Mirabet
Vicente
V
Blanquer
Amando
A
Delgado-Rosas
Francisco
F
Planelles
Dolores
D
Andrade
Margarita
M
Carbonell-Uberos
Francisco
F
Soler
M Angeles
MA
Roig
Roberto
R
D001331:Automation / Q000295:instrumentation*; D000094902:Blood Banking / Q000379:methods*; D002467:Cell Nucleus / Q000378:metabolism; D048429:Cell Size; D004912:Erythrocytes / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans