title: Ultrastructural localization of cochlin in the rat cochlear duct.
abstract: Cochlin, a product of the COCH gene, is a major constituent of the inner ear extracellular matrix. Type II collagen, a protein that contributes to structural stability, is also a component of this extracellular matrix. In this study, using the postembedding immunogold method, we demonstrate the localization of cochlin and type II collagen in the cochlear duct at the ultrastructural level. The immunolabeling of cochlin was observed in the fibrillar substance in the spiral limbus, beneath the inner sulcus cells, and in the basilar membrane, the spiral prominence and the spiral ligament. Immunolabeling of type II collagen was observed in the same fibrillar substance in the extracellular matrix of the cochlear duct. This localization of cochlin is consistent with the expected localization of type II collagen. The localization of cochlin and type II collagen indicates the important roles played by these proteins in the hearing process.
authors.affiliation: Department of Otolaryngology, Hamamatsu University School of Medicine, Hamamatsu, Japan. seijih@hama-med.ac.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Hosokawa
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Mizuta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Maki
authors.initials: M
authors.lastname: Arai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mineta
authors.forename: Susumu
authors.initials: S
authors.lastname: Shindo
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ikezono
mesh_terms: D000818:Animals; D001489:Basilar Membrane / Q000033:anatomy & histology; D003053:Cochlear Duct / Q000033:anatomy & histology*; D024043:Collagen Type II / Q000032:analysis; D005109:Extracellular Matrix / Q000000981:diagnostic imaging; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D055493:Spiral Ligament of Cochlea / Q000033:anatomy & histology; D014463:Ultrasonography
chemical_list: C529734:Coch protein, rat; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins