Evaluation of a condensation nucleation light scattering detector for the analysis of synthetic polymer by supercritical fluid chromatography.
A condensation nucleation light scattering detector (CNLSD) was adapted for use as a detector for supercritical fluid chromatography. The performance of the CNLSD was evaluated and compared to evaporative light-scattering detector (ELSD) using a well-defined equimass mixture of uniform poly(ethylene glycol) oligomers and a certified reference material of poly(ethylene glycol) 1000. The CNLSD was able to detect a 10 times less concentrated solution of uniform oligomers compared to the ELSD. The quantitativeness of CNLSD was high enough to obtain the molecular mass distribution of poly(ethylene glycol) 1000 without any calibrations; on the other hand, the original data measured by ELSD was about 4% smaller than the certified value of poly(ethylene glycol) 1000. The CNLSD was suitable for supercritical fluid chromatography as a mass concentration detector.
Takahashi
Kayori
K
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Kinugasa
Shinichi
S
Yoshihara
Ryo
R
Nakanishi
Akira
A
Mosing
Renee K
RK
Takahashi
Rheo
R
D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods*; D008027:Light; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D013696:Temperature; D014835:Volatilization
D011092:Polyethylene Glycols