A rapid and economic in-house DNA purification method using glass syringe filters.
BACKGROUND
Purity, yield, speed and cost are important considerations in plasmid purification, but it is difficult to achieve all of these at the same time. Currently, there are many protocols and kits for DNA purification, however none maximize all four considerations.


RESULTS
We now describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters. Plasmid yield and quality as determined by enzyme digestion and transfection efficiency were equivalent to the expensive commercial kits. Importantly, the time required for purification was much less than that required using a commercial kit.


CONCLUSIONS
This method provides DNA yield and quality similar to that obtained with commercial kits, but is more rapid and less costly.
Kim
Yun-Cheol
YC
Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA. kyc8402@ucla.edu
Morrison
Sherie L
SL
D001419:Bacteria / Q000235:genetics; D002460:Cell Line; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005374:Filtration / Q000295:instrumentation*; D005821:Genetic Techniques / Q000191:economics; D005898:Glass; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013594:Syringes; D014162:Transfection
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic