Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis: proteomic analysis.
To detect serum biomarkers associated with disease activity in relapsing-remitting multiple sclerosis (MS). We studied serum low-molecular peptide profiling of MS patients and normal controls comprehensively by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Serum level of 1741 Da peptide was increased at the time of clinical relapse in patients than in normal controls and returned toward normal during remission. Tandem mass spectrometry analysis revealed that the peptide was a fragment of complement C4 (NGFKSHALQLNNRQI). This fragment peptide could be a possible marker of disease activity. It may reflect complement activation in the pathogenesis of MS.
Sawai
Setsu
S
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. ssawai@yj8.so-net.ne.jp
Umemura
Hiroshi
H
Mori
Masahiro
M
Satoh
Mamoru
M
Hayakawa
Sei
S
Kodera
Yoshio
Y
Tomonaga
Takeshi
T
Kuwabara
Satoshi
S
Nomura
Fumio
F
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D003167:Complement Activation / Q000276:immunology; D003181:Complement C4 / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000276:immunology*; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
D015415:Biomarkers; D003181:Complement C4; D010446:Peptide Fragments