title: Purification, crystallization and preliminary X-ray diffraction experiments on the breakage-reunion domain of the DNA gyrase from Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis DNA gyrase, a nanomachine that is involved in the regulation of DNA topology, is the only type II topoisomerase present in this organism and hence is the sole target for fluoroquinolone action. The breakage-reunion domain of the A subunit plays an essential role in DNA binding during the catalytic cycle. Two constructs of 53 and 57 kDa (termed GA53BK and GA57BK) corresponding to this domain have been overproduced, purified and crystallized. Diffraction data were collected from four crystal forms. The resolution limits ranged from 4.6 to 2.7 angstrom depending on the crystal form. The best diffracting crystals belonged to space group C2, with a biological dimer in the asymmetric unit. This is the first report of the crystallization and preliminary X-ray diffraction analysis of the breakage-reunion domain of DNA gyrase from a species containing one unique type II topoisomerase.
authors.affiliation: Département de Biologie Structurale et Chimie, URA du CNRS, Institut Pasteur, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Piton
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Matrat
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Petrella
authors.forename: Vincent
authors.initials: V
authors.lastname: Jarlier
authors.forename: Alexandra
authors.initials: A
authors.lastname: Aubry
authors.forename: Claudine
authors.initials: C
authors.lastname: Mayer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D027081:DNA Gyrase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D021122:Protein Subunits; D027081:DNA Gyrase