title: Capacitance-based real time monitoring of receptor-mediated endocytosis.
abstract: Receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. In this study, we developed a capacitance sensor to monitor receptor-mediated endocytosis in real time. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination between receptor-mediated endocytosis and non-specific pinocytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening. We showed that the capacitance sensor array could rapidly identify antibodies or ligands with high specificity for target molecules. We propose that the capacitance sensor array will provide a valuable tool for high-throughput screening.
authors.affiliation: Graduate Program for Nanomedical Science and Technology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Rimi
authors.initials: R
authors.lastname: Lee
authors.forename: Pyung Hwan
authors.initials: PH
authors.lastname: Kim
authors.forename: Joung-Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: Kwon
authors.initials: K
authors.lastname: Oh-Joon
authors.forename: Kyujung
authors.initials: K
authors.lastname: Kim
authors.forename: Donghyun
authors.initials: D
authors.lastname: Kim
authors.forename: Chae-Ok
authors.initials: CO
authors.lastname: Yun
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Yoo
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003199:Computer Systems; D025802:Electric Capacitance; D004566:Electrodes; D004705:Endocytosis / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D010992:Plethysmography, Impedance / Q000295:instrumentation*; D011956:Receptors, Cell Surface / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011956:Receptors, Cell Surface