Effect of Thai 'koi-hoi' food flavoring on the viability and infectivity of the third-stage larvae of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae).
The effect of the food flavoring of 'koi-hoi', a popular Thai snail dish, on the viability and infectivity of Angiostrongylus (=Parastrongylus) cantonensis third-stage larvae was assessed in a mouse model. Groups of 50 each of actively moving, non-motile coiled, and extended larvae were obtained from experimentally infected snail meat, after one-hour exposure to standard 'koi-hoi' flavoring. These larvae and groups of 50 unexposed moving larvae (control) were individually fed to each group of three experimental BALB/c mice. The effect on Angiostrongylus worm burden was measured after 3 weeks of infection. Infectivity of the motile larvae after exposure to 'koi-hoi' food flavoring was 38 + or - 5.29%. This was highly significantly lower than the infectivity (62 + or - 7.21%) of the control (unexposed) third-stage larvae (chi(2) = 17.28, P < 0.001). In the non-motile larvae resulting from exposure to the food flavoring, no adult worm was recovered from the extended larvae, indicating that they were no longer alive and unable to cause infection. A small proportion (3.33 + or - 2.31%) of the coiled larvae developed into young adult worms, indicating that mobility alone is not a definitive indicator of viability. The present study confirms that the food flavoring components of 'koi-hoi' dish adversely affect the viability and infectivity of A. cantonensis larvae. Exposure of the third-stage larvae to 'koi-hoi' food flavoring resulted in decreased viability and eventually death. Prolonged treatment with food flavoring to inactivate/immobilize and then kill the infective, third-stage larvae of A. cantonensis in snail meat prior to consumption may be one of the possible economical means of reducing human infection.
Eamsobhana
Praphathip
P
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. sipes@mucc.mahidol.ac.th
Yoolek
Adisak
A
Yong
Hoi-Sen
HS
D017185:Angiostrongylus cantonensis / Q000187:drug effects* / Q000472:pathogenicity; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D005421:Flavoring Agents / Q000494:pharmacology*; D016266:Food Parasitology; D006801:Humans; D007814:Larva / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D014774:Virulence
D000871:Anthelmintics; D005421:Flavoring Agents