[Growth characteristics of Campylobacter jejuni on different culture media and their expression of outer membrane proteins].
OBJECTIVE
To evaluate the biological characteristics of Campylobacter jejuni (CJ) cultured on different culture media and their expression abundance of outer membrane proteins (OMPs).


METHODS
CJ was cultured on the improved Bull's medium yolk agar, improved Bull's blood agar or improved Bull's agar for 48 h. The biological characteristics of the bacteria, including the colony feature, morphology, motility, biochemistry, and results of indirect fluorescence test were observed and compared. OMP of the cultured CJ was extracted using 0.2 mol/L and glycine-hydrochloride buffered solution (pH 2.2) and identified by SDS-PAGE to compare the expression abundance of the OMPs with molecular weight of 28-31 kD.


RESULTS
CJ exhibited typical biological characteristics with larger cell body and more rapid growth on improved Bull's medium yolk agar than those on improved Bull's blood agar and improved Bull's agar. The bacteria grown on improved Bull's medium yolk agar showed also greater expression abundance of the OMPs with molecule mass between 28 kD and 31 kD.


CONCLUSIONS
Improved Bull's medium yolk agar allows rapid growth of CJ with typical biological characteristics and enhanced expression of the OMPs with molecular weight of 28 -31 kD, and can be widely used in CJ subunit vaccine development, CJ epidemiological survey, CJ food safety examination, and CJ quarantine.
Feng
Sheng-Jun
SJ
Department of Microbiology and Immunology, Guangdong Medical College, Dongguan 523808, China. fengshj822@yahoo.com.cn
Wu
Li-Dan
LD
Sun
Wan-Bang
WB
Xiao
Zheng
Z
Mi
Na
N
Liu
Fang
F
Chen
Qun
Q
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media