title: Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen (HAp/Col) and fibroblast growth factor-2 (FGF-2).
abstract: Articular cartilage has a limited capacity for self-renewal. This article reports the development of a porous hydroxyapatite/collagen (HAp/Col) scaffold as a bone void filler and a vehicle for drug administration. The scaffold consists of HAp nanocrystals and type I atelocollagen. The purpose of this study was to investigate the efficacy of porous HAp/Col impregnated with FGF-2 to repair large osteochondral defects in a rabbit model. Ninety-six cylindrical osteochondral defects 5 mm in diameter and 5 mm in depth were created in the femoral trochlear groove of the right knee. Animals were assigned to one of four treatment groups: porous HAp/Col impregnated with 50 microl of FGF-2 at a concentration of 10 or 100 microg/ml (FGF10 or FGF100 group); porous HAp/Col with 50 microl of PBS (HAp/Col group); and no implantation (defect group). The defect areas were examined grossly and histologically. Subchondral bone regeneration was quantified 3, 6, 12, and 24 weeks after surgery. Abundant bone formation was observed in the HAp/Col implanted groups as compared to the defect group. The FGF10 group displayed not only the most abundant bone regeneration but also the most satisfactory cartilage regeneration, with cartilage presenting a hyaline-like appearance. These findings suggest that porous HAp/Col with FGF-2 augments the cartilage repair process.
authors.affiliation: Department of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Maehara
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sotome
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Yoshii
authors.forename: Ichiro
authors.initials: I
authors.lastname: Torigoe
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sugata
authors.forename: Masato
authors.initials: M
authors.lastname: Yuasa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hirano
authors.forename: Naomi
authors.initials: N
authors.lastname: Mochizuki
authors.forename: Masanori
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shinomiya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okawa
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000601:surgery; D002357:Cartilage Diseases / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000601:surgery; D002358:Cartilage, Articular / Q000000981:diagnostic imaging / Q000601:surgery; D003094:Collagen / Q000494:pharmacology*; D003131:Combined Modality Therapy; D016503:Drug Delivery Systems / Q000379:methods; D017886:Durapatite / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging / Q000601:surgery; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008297:Male; D057139:Nanofibers; D011183:Postoperative Complications; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D055114:X-Ray Microtomography
chemical_list: D016222:Fibroblast Growth Factor 2; D003094:Collagen; D017886:Durapatite