Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development.
Mining gene-expression-profiling data identified a novel gene that is specifically expressed in preimplantation embryos. Hmgpi, a putative chromosomal protein with two high-mobility-group boxes, is zygotically transcribed during zygotic genome activation, but is not transcribed postimplantation. The Hmgpi-encoded protein (HMGPI), first detected at the 4-cell stage, remains highly expressed in pre-implantation embryos. Interestingly, HMGPI is expressed in both the inner cell mass (ICM) and the trophectoderm, and translocated from cytoplasm to nuclei at the blastocyst stage, indicating differential spatial requirements before and after the blastocyst stage. siRNA (siHmgpi)-induced reduction of Hmgpi transcript levels caused developmental loss of preimplantation embryos and implantation failures. Furthermore, reduction of Hmgpi prevented blastocyst outgrowth leading to generation of embryonic stem cells. The siHmgpi-injected embryos also lost ICM and trophectoderm integrity, demarcated by reduced expressions of Oct4, Nanog and Cdx2. The findings implicated an important role for Hmgpi at the earliest stages of mammalian embryonic development.
Yamada
Mitsutoshi
M
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo, Japan.
Hamatani
Toshio
T
Akutsu
Hidenori
H
Chikazawa
Nana
N
Kuji
Naoaki
N
Yoshimura
Yasunori
Y
Umezawa
Akihiro
A
D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000378:metabolism; D010064:Embryo Implantation; D004622:Embryo, Mammalian; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D024002:HMGB Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy
D024002:HMGB Proteins; C548051:Hmgpi protein, mouse; D050814:Octamer Transcription Factor-3