[Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in post-hepatitis B cirrhosis].
OBJECTIVE
To investigate the relationship between single-nucleotide polymorphisms (SNPs) of interleukin-10 (IL-10) and syndrome types of traditional Chinese medicine (TCM) in posthepatitis B cirrhosis.


METHODS
The genotypes of IL-10-592 A/C, -819 C/T and -1082 G/A sites were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and polymerase chain reaction-ligase detection reaction (PCR-LDR) combined with the sequencing analysis in 226 cases of posthepatitis B cirrhosis. The genotype and allele frequency distribution, and the relationship between the SNPs and TCM syndromes were analyzed.


RESULTS
The frequency of allele C at IL-10-819 point in spleen deficiency with overabundance of dampness syndrome was significantly higher than that in non-spleen deficiency with overabundance of dampness (P<0.01) syndrome, and genotype TT in liver stagnation syndrome was significantly higher than that in non-liver stagnation syndrome (P<0.05). There was no significant relationship between the polymorphisms of IL-10-592 A/C, -1082 G/A and the TCM syndromes in posthepatitis B cirrhosis (P>0.05).


CONCLUSIONS
In patients with posthepatitis B cirrhosis, allele C in IL-10-819 locus may be related to spleen deficiency with overabundance of dampness syndrome, and TT genotype in IL-10-819 locus may be related to liver stagnation syndrome.
Jiang
Zai-lai
ZL
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhang
Wei
W
Zhang
Hui
H
Liu
Yi-bo
YB
Li
Qing-ya
QY
Su
Shi-bing
SB
D000293:Adolescent; D000328:Adult; D000368:Aged; D003937:Diagnosis, Differential; D005260:Female; D019694:Hepatitis B, Chronic / Q000150:complications; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008103:Liver Cirrhosis / Q000145:classification / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
D016753:Interleukin-10