title: A lateral flow-based ultra-sensitive p24 HIV assay utilizing fluorescent microparticles.
abstract: The current demand for HIV diagnostic tests in resource-limited settings is not being fully addressed by the currently available nucleic acid test or enzyme-linked immunosorbent assay-based p24 antigen assays. This is primarily due to their high cost, their requirement for controlled laboratory conditions and/or personnel, or the relatively long turn-around time for test results. Self performing lateral flow assays address all of these issues but to date have not reached the level of analytical sensitivity for HIV p24 demonstrated by current generation enzyme-linked immunosorbent assay technology. This report presents an initial prototype lateral flow assay for HIV p24 antigen capable of achieving subpicogram per milliliter limits of analytical sensitivity and requiring less than 40 minutes to yield results.
authors.affiliation: Department of Biomedical Engineering, Center for Innovation in Global Health Technologies, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA. arman@northwestern.edu
authors.forename: Arman
authors.initials: A
authors.lastname: Nabatiyan
authors.forename: Michael A
authors.initials: MA
authors.lastname: Baumann
authors.forename: Zaheer
authors.initials: Z
authors.lastname: Parpia
authors.forename: David
authors.initials: D
authors.lastname: Kelso
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000737:chemistry; D016655:HIV Core Protein p24 / Q000097:blood* / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D016655:HIV Core Protein p24