Identification of messenger RNA substrates for mouse T-STAR.
Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3' untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis.
Zhang
L Y
LY
Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, P. R. China.
Zeng
M
M
Chen
P
P
Sun
H Q
HQ
Tao
D C
DC
Liu
Y Q
YQ
Lin
L
L
Yang
Y
Y
Zhang
S Z
SZ
Ma
Y X
YX
D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000737:chemistry* / Q000378:metabolism
C484252:Fabp9 protein, mouse; D050556:Fatty Acid-Binding Proteins; C486528:Khdrbs3 protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins