Omental fat expression of adiponectin and adiponectin receptors in non-obese women with PCOS: a preliminary study.
Adiponectin is regarded as a possible link between adiposity and insulin resistance. The aim of the study was to determine adipose levels of mRNA for adiponectin and adiponectin receptors (AdipoR) in non-obese women with polycystic ovary syndrome (PCOS), and to assess whether the cytokine and receptors are related to insulin resistance in PCOS. Adipose tissue obtained from eight non-obese women with PCOS [body mass index (BMI) <27 kg/m(2) as cut-off point] was analysed. Levels of mRNA for adiponectin, AdipoR1 and 2 were quantified using the semi-quantitative reverse transcriptase-polymerase chain reaction. Eight non-obese, age- and BMI-matched healthy women served as controls. The level of adiponectin mRNA in non-obese women with PCOS were lower than in controls, but the difference was not statistically significant. However, AdipoR1 and 2 mRNA levels in non-obese women with PCOS were significantly lower than in controls. There was a significant negative correlation between 2 h insulin levels and AdipoR1 or AdipoR2 mRNA levels in non-obese women with PCOS ( r = 0.45 and 0.52 respectively, P < 0.05). The present study demonstrates that adiponectin receptor expression is down-regulated by hyperinsulinaemia in non-obese women with PCOS, resulting in adiponectin resistance.
Seow
Kok-Min
KM
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Tsai
Yieh-Loong
YL
Juan
Chi-Chang
CC
Lin
Yu-Hung
YH
Hwang
Jiann-Loung
JL
Ho
Low-Tone
LT
D052242:Adiponectin / Q000096:biosynthesis*; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance; D009765:Obesity / Q000378:metabolism; D009852:Omentum / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000096:biosynthesis*
C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin