Comparison of DNA extraction methods for multiplex polymerase chain reaction.
We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes. The DNA quantity, DNA purity (A(260/280)), PCR inhibition ratio, and mitochondrial DNA/genomic DNA ratio were measured to compare the extraction methods. The different extraction methods resulted in variable DNA quantity and purity, but there were no significant differences in the efficiency of multiplex PCR and oligomicroarray signals after single-base extension on the arrayed primer extension 2 (APEX-2).
Viltrop
Triin
T
Department of Biotechnology, IMCB, University of Tartu, Tartu, Estonia. tviltrop@ut.ee
Krjutskov
Kaarel
K
Palta
Priit
P
Metspalu
Andres
A
D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry
D004247:DNA