Nitric oxide releasing nanoparticles are therapeutic for Staphylococcus aureus abscesses in a murine model of infection.
Staphylococcus aureus (SA) is a leading cause of a diverse spectrum of bacterial diseases, including abscesses. Nitric oxide (NO) is a critical component of the natural host defense against pathogens such as SA, but its therapeutic applications have been limited by a lack of effective delivery options. We tested the efficacy of a NO-releasing nanoparticle system (NO-np) in methicillin-resistant SA (MRSA) abscesses in mice. The results show that the NO-np exert antimicrobial activity against MRSA in vitro and in abscesses. Topical or intradermal NO-np treatment of abscesses reduces the involved area and bacterial load while improving skin architecture. Notably, we evaluated pro- and anti-inflammatory cytokines that are involved in immunomodulation and wound healing, revealing that NO-np lead to a reduction in angiogenesis preventing bacterial dissemination from abscesses. These data suggest that NO-np may be useful therapeutics for microbial abscesses.
Han
George
G
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY, USA.
Martinez
Luis R
LR
Mihu
Mircea Radu
MR
Friedman
Adam J
AJ
Friedman
Joel M
JM
Nosanchuk
Joshua D
JD
D000038:Abscess / Q000188:drug therapy / Q000382:microbiology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry; D016503:Drug Delivery Systems; D004817:Epidermis / Q000378:metabolism; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009389:Neovascularization, Pathologic; D009569:Nitric Oxide / Q000008:administration & dosage*; D013211:Staphylococcus aureus / Q000378:metabolism*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D009569:Nitric Oxide; D003094:Collagen; D007770:L-Lactate Dehydrogenase