title: Elimination of false-positive results in a luminex assay for pneumococcal antibodies.
abstract: In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA. jerry.pickering@aruplab.com
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Pickering
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Larson
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Martins
authors.forename: Susan S
authors.initials: SS
authors.lastname: Copple
authors.forename: Harry R
authors.initials: HR
authors.lastname: Hill
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002417:Cattle; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D012710:Serum Albumin, Bovine