title: Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes.
abstract: Circulating NAMPT (PBEF/visfatin) has pleiotropic functions and is secreted from adipocytes. Since it is doubtful whether serum levels can be explained by secretion from adipocytes alone, we asked whether hepatocytes are also able to liberate NAMPT. Using HepG2 cells and primary rat and human hepatocytes, release of NAMPT into the cell culture supernatant was found to occur constitutively in a time-dependent manner. In primary human hepatocytes, secretion within 24h was far higher than the cellular content, but was neither influenced by inhibitors of secretion nor by glucose, insulin or TNFalpha. As determined by size exclusion chromatography, HepG2 lysates and supernatants primarily contained the dimeric form of NAMPT which exhibited similar in vitro specific enzymatic activity. In primary human hepatocytes, secreted NAMPT was less active. Our results demonstrate that human hepatocytes are a potential source of circulating NAMPT.
authors.affiliation: University Hospital for Children and Adolescents, University of Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Garten
authors.forename: S
authors.initials: S
authors.lastname: Petzold
authors.forename: A
authors.initials: A
authors.lastname: Barnikol-Oettler
authors.forename: A
authors.initials: A
authors.lastname: Körner
authors.forename: W E
authors.initials: WE
authors.lastname: Thasler
authors.forename: J
authors.initials: J
authors.lastname: Kratzsch
authors.forename: W
authors.initials: W
authors.lastname: Kiess
authors.forename: R
authors.initials: R
authors.lastname: Gebhardt
mesh_terms: D000818:Animals; D001775:Blood Circulation; D002460:Cell Line; D003470:Culture Media; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood / Q000378:metabolism*; D051381:Rats
chemical_list: D003470:Culture Media; D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human