Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7.
Poxviruses are DNA viruses that express numerous proteins to subvert the host immune response. Vaccinia virus protein K7 adopts a Bcl-2 fold and displays structural and functional similarities to Toll-like receptor antagonist A52. Both proteins interact with IRAK2 and TRAF6 and suppress TLR-dependent NF-kappaB activation. However, unlike A52, K7 also forms a complex with RNA helicase DDX3 and antagonizes interferon-beta promoter induction. We have narrowed the K7 binding site to an N-terminal peptide motif of DDX3 ahead of its core RNA-helicase domains. The crystal structure of full-length K7 in complex with the DDX3 peptide reveals a thumblike projection of tandem phenalyalanine residues of DDX3 into a deep hydrophobic cleft. Mutagenesis of these phenylalanines abolishes the effects of DDX3 on interferon-beta promoter induction. The structure of K7-DDX3 reveals a novel binding mode by a viral Bcl-2 protein that antagonizes a key pathway in innate immunity.
Oda
Shun-Ichiro
S
School of Biochemistry and Immunology, Trinity College, Dublin, Dublin 2, Ireland.
Schröder
Martina
M
Khan
Amir R
AR
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D003460:Crystallization; D053487:DEAD-box RNA Helicases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D016899:Interferon-beta / Q000037:antagonists & inhibitors; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016415:Sequence Alignment; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism; D014616:Vaccinia virus / Q000737:chemistry*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D048029:TNF Receptor-Associated Factor 6; D014764:Viral Proteins; D016899:Interferon-beta; D053592:Interleukin-1 Receptor-Associated Kinases; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases