title: Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).
abstract: Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
authors.affiliation: Indian Institute of Spices Research, Calicut, Kerala, India.
authors.forename: S
authors.initials: S
authors.lastname: Siju
authors.forename: K
authors.initials: K
authors.lastname: Dhanya
authors.forename: S
authors.initials: S
authors.lastname: Syamkumar
authors.forename: B
authors.initials: B
authors.lastname: Sasikumar
authors.forename: T E
authors.initials: TE
authors.lastname: Sheeja
authors.forename: A I
authors.initials: AI
authors.lastname: Bhat
authors.forename: V A
authors.initials: VA
authors.lastname: Parthasarathy
mesh_terms: D030024:Curcuma / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D004586:Electrophoresis; D020224:Expressed Sequence Tags; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D018744:DNA, Plant