title: Tc17 CD8 T cells: functional plasticity and subset diversity.
abstract: IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-gamma and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-gamma-positive or IL-17/IFN-gamma-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-gamma-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-gamma-producing cells are desired.
authors.affiliation: Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
authors.forename: Hung-Rong
authors.initials: HR
authors.lastname: Yen
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Harris
authors.forename: Satoshi
authors.initials: S
authors.lastname: Wada
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Grosso
authors.forename: Derese
authors.initials: D
authors.lastname: Getnet
authors.forename: Monica V
authors.initials: MV
authors.lastname: Goldberg
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Liang
authors.forename: Tullia C
authors.initials: TC
authors.lastname: Bruno
authors.forename: Kristin J
authors.initials: KJ
authors.lastname: Pyle
authors.forename: Siaw-Li
authors.initials: SL
authors.lastname: Chan
authors.forename: Robert A
authors.initials: RA
authors.lastname: Anders
authors.forename: Cornelia L
authors.initials: CL
authors.lastname: Trimble
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Adler
authors.forename: Tzou-Yien
authors.initials: TY
authors.lastname: Lin
authors.forename: Drew M
authors.initials: DM
authors.lastname: Pardoll
authors.forename: Ching-Tai
authors.initials: CT
authors.lastname: Huang
authors.forename: Charles G
authors.initials: CG
authors.lastname: Drake
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D050796:STAT3 Transcription Factor; D007371:Interferon-gamma