Capacitance-based real time monitoring of receptor-mediated endocytosis.
Receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. In this study, we developed a capacitance sensor to monitor receptor-mediated endocytosis in real time. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination between receptor-mediated endocytosis and non-specific pinocytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening. We showed that the capacitance sensor array could rapidly identify antibodies or ligands with high specificity for target molecules. We propose that the capacitance sensor array will provide a valuable tool for high-throughput screening.
Lee
Rimi
R
Graduate Program for Nanomedical Science and Technology, Yonsei University, Seoul, Republic of Korea.
Kim
Pyung Hwan
PH
Choi
Joung-Woo
JW
Oh-Joon
Kwon
K
Kim
Kyujung
K
Kim
Donghyun
D
Yun
Chae-Ok
CO
Yoo
Kyung-Hwa
KH
D015374:Biosensing Techniques / Q000295:instrumentation*; D003199:Computer Systems; D025802:Electric Capacitance; D004566:Electrodes; D004705:Endocytosis / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D010992:Plethysmography, Impedance / Q000295:instrumentation*; D011956:Receptors, Cell Surface / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011956:Receptors, Cell Surface