title: Up-regulation of ferritin ubiquitination in skeletal muscle of transgenic rats bearing the G93A hmSOD1 gene mutation.
abstract: In the present study we measured the levels of protein carbolnyls and the H and L subunits of ferritin in three hind limb muscles, [Extensor digitorum longus, Tibialis anterior and Soleus] of transgenic rats bearing the G93A hmSOD1 gene and of their non-transgenic littermates. All of the muscles from the transgenic animals showed significantly higher protein carbonyl levels, compared to the respective muscles from control non-transgenic animals. In two muscles (Tibialis anterior and Soleus) from transgenic rats, both L and H subunits of ferritin were upregulated. Moreover, we observed that the electrophoretic mobility of both ferritin subunits was retarded which indicates their post-translational modification. Ferritin immunoprecipitation experiments show an increased ubiquitination of both H and L ferritin in all muscles from the transgenic animals. Our data show for the first time that ferritin ubiquitination could be responsible for oxidative stress in muscles of rats bearing the G93A hmSOD1, consequently ferritin is not able to control the labile iron pool.
authors.affiliation: Department of Bioenergetics and Physiology of Exercise, Medical University of Gdansk, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Halon
authors.forename: Alicja
authors.initials: A
authors.lastname: Sielicka-Dudzin
authors.forename: Michal
authors.initials: M
authors.lastname: Wozniak
authors.forename: Wiesław
authors.initials: W
authors.lastname: Ziolkowski
authors.forename: Walenty
authors.initials: W
authors.lastname: Nyka
authors.forename: Magdalena
authors.initials: M
authors.lastname: Herbik
authors.forename: Pawel
authors.initials: P
authors.lastname: Grieb
authors.forename: Adam
authors.initials: A
authors.lastname: Figarski
authors.forename: Jedrzej
authors.initials: J
authors.lastname: Antosiewicz
mesh_terms: D000818:Animals; D003124:Colorimetry; D004586:Electrophoresis; D005293:Ferritins / Q000378:metabolism*; D006614:Hindlimb / Q000378:metabolism; D047468:Immunoprecipitation; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism*; D009929:Organ Size / Q000235:genetics; D050050:Protein Carbonylation / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D054875:Ubiquitination / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D005293:Ferritins; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1