title: Structure of an Escherichia coli N-acetyl-D-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 angstrom resolution.
abstract: The structure of a mutant variant of Escherichia coli N-acetyl-d-neuraminic acid lyase (NAL), E192N, in complex with pyruvate has been determined in a new crystal form. It crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 78.3, b = 108.5, c = 148.3 angstrom. Pyruvate has been trapped in the active site as a Schiff base with the catalytic lysine (Lys165) without the need for reduction. Unlike the previously published crystallization conditions for the wild-type enzyme, in which a mother-liquor-derived sulfate ion is strongly bound in the catalytic pocket, the low-salt conditions described here will facilitate the determination of further E. coli NAL structures in complex with other activesite ligands.
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, England.
authors.forename: Ivan
authors.initials: I
authors.lastname: Campeotto
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Carr
authors.forename: Chi H
authors.initials: CH
authors.lastname: Trinh
authors.forename: Adam S
authors.initials: AS
authors.lastname: Nelson
authors.forename: Alan
authors.initials: A
authors.lastname: Berry
authors.forename: Simon E V
authors.initials: SE
authors.lastname: Phillips
authors.forename: Arwen R
authors.initials: AR
authors.lastname: Pearson
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008190:Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D019289:Pyruvic Acid / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; D009438:Neuraminic Acids; D019289:Pyruvic Acid; D008190:Lyases