title: Evidence of recombination in Hepatitis C Virus populations infecting a hemophiliac patient.
abstract: OBJECTIVE
  Hepatitis C virus (HCV) infection is an important cause of morbidity and mortality in patients affected by hereditary bleeding disorders. HCV, as others RNA virus, exploit all possible mechanisms of genetic variation to ensure their survival, such as recombination and mutation. In order to gain insight into the genetic variability of HCV virus strains circulating in hemophiliac patients, we have performed a phylogenetic analysis of HCV strains isolated from 10 patients with this kind of pathology.
  
  
  METHODS
  Putative recombinant sequence was identified with the use of GARD program. Statistical support for the presence of a recombination event was done by the use of LARD program.
  
  
  RESULTS
  A new intragenotypic recombinant strain (1b/1a) was detected in 1 out of the 10 hemophiliac patient studied. The recombination event was located at position 387 of the HCV genome (relative to strain AF009606, sub-type 1a) corresponding to the core gene region.
  
  
  CONCLUSIONS
  Although recombination may not appear to be common among natural populations of HCV it should be considered as a possible mechanism for generating genetic diversity in hemophiliacs patients.
authors.affiliation: Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. pmoreno@cin.edu.uy
authors.forename: Pilar
authors.initials: P
authors.lastname: Moreno
authors.forename: Macarena
authors.initials: M
authors.lastname: Alvarez
authors.forename: Lilia
authors.initials: L
authors.lastname: López
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Moratorio
authors.forename: Didier
authors.initials: D
authors.lastname: Casane
authors.forename: Matías
authors.initials: M
authors.lastname: Castells
authors.forename: Silvia
authors.initials: S
authors.lastname: Castro
authors.forename: Juan
authors.initials: J
authors.lastname: Cristina
authors.forename: Rodney
authors.initials: R
authors.lastname: Colina
mesh_terms: D016000:Cluster Analysis; D006467:Hemophilia A / Q000150:complications*; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral