title: Involvement of Src tyrosine kinase in Escherichia coli invasion of human brain microvascular endothelial cells.
abstract: Invasion of brain microvascular endothelial cells is a prerequisite for successful crossing of the blood-brain barrier by Escherichia coli (E. coli), but the underlying mechanism remains unclear. Here we showed activation of Src tyrosine kinase in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC). E. coli invasion of HBMEC and the E. coli-induced rearrangement of actin filaments were blocked by Src inhibitors. Overexpression of dominant-negative Src in HBMEC significantly attenuated E. coli invasion and the concomitant actin filaments rearrangement. Furthermore, E. coli K1-triggered phosphatidylinositol 3-kinase (PI3K) activation in HBMEC was effectively blocked by Src inhibitors and dominant-negative Src. These results demonstrated the involvement of Src and its interaction with PI3K in E. coli K1 invasion of HBMEC.
authors.affiliation: Department of Developmental Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Heping Distric, Shenyang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhao
authors.forename: Jin-Chun
authors.initials: JC
authors.lastname: Yan
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Ren
authors.forename: Wen-Gang
authors.initials: WG
authors.lastname: Fang
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: De-Shu
authors.initials: DS
authors.lastname: Shang
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000201:enzymology / Q000382:microbiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism / Q000382:microbiology; D004730:Endothelium, Vascular / Q000201:enzymology / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000201:enzymology*; D006801:Humans; D055806:Microvessels / Q000201:enzymology / Q000382:microbiology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000199:Actins; D019869:Phosphatidylinositol 3-Kinases; D019061:src-Family Kinases