title: An auxin-based degron system for the rapid depletion of proteins in nonplant cells.
abstract: Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function.
authors.affiliation: Department of Biological Sciences, Osaka University, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Nishimura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fukagawa
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Takisawa
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kakimoto
authors.forename: Masato
authors.initials: M
authors.lastname: Kanemaki
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; D011506:Proteins; D049452:Green Fluorescent Proteins; D044843:SKP Cullin F-Box Protein Ligases