Orthotopic testicular transplantation in mice.
A method of revascularized orthotopic testicular transplantation (OTT) was developed in mice. The left testis was selected as donor graft for the operation due to less variation in anatomy. There were three groups: 1) a control group (n=24), 2) a group of castrated mice (n=24), and 3) a group in which OTT (n=24) was performed. Morphologically, the transplanted testes showed active spermatogenesis and normal structure of epididymis at 4 and 5 weeks. The function of the transplants was examined by RIA at designed time points. LH, FSH, and testosterone showed return to normal levels at 4 weeks. To our knowledge, this is the first report of successful revascularized OTT in mice. The model may prove useful in research in reproductive medicine, especially using knockout and transgenic mice.
Li
Jianhui
J
Key Laboratory of Organ Transplantation, Key Laboratory of Combined Multi-organ Transplantation, Department of Hepatobiliary and Pancreatic Surgery, Ministry of Public Health, Medical College, First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, People's Republic of China.
Savolainen
Hannu
H
Tan
Fuqing
F
Zheng
Shusen
S
D000818:Animals; D004822:Epididymis / Q000637:transplantation; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009919:Orchiectomy; D011863:Radioimmunoassay; D013091:Spermatogenesis; D013737:Testis / Q000098:blood supply / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D013739:Testosterone / Q000097:blood; D013997:Time Factors
D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone