title: Room temperature pulsatile perfusion of renal allografts with Lifor compared with hypothermic machine pump solution.
abstract: This pilot study compared the use of the Lifor Organ Preservation Medium (RTLF) at room temperature with hypothermic Belzer machine preservation solution (CMPS) and room in vitro temperature Belzer machine preservation solution (RTMPS) in a porcine model of uncontrolled donation after cardiac death (DCD). In this study, 5 porcine kidneys for each perfusate group were recovered under a DCD protocol. The kidneys were recovered, flushed, and placed onto a renal preservation system following standard perfusion procedures. The average flow rate for CMPS was 36.2 +/- 7.2549 mL/min, RTMPS was 90.2 +/- 9.7159 mL/min, and RTLF was 103.1 +/- 5.1108 mL/min. The average intrarenal resistance for CMPS was 1.33 +/- 0.1709 mm Hg/mL per minute, RTMPS was 0.84 +/- 0.3586 and RTLF was 0.39 +/- 0.04. All perfusion parameters were statistically significant (P < .05) at all time points for the CMPS when compared with both RTMPS and RTLF. All perfusion parameters for RTMPS and RTLF were equivalent for the first 12 hours; thereafter, RTLF became significantly better than RTMPS at 18 and 24 hours. It appears that both RTMPS and RTLF have equivalent perfusion characteristic for the initial 12 hours of perfusion, but LF continues to maintain a low resistance and high flow up to 24 hours. The results of this pilot study indicate that RTLF may represent a better alternative to pulsatile perfusion with CMPS and requires validation in an in vivo large animal transplant model.
authors.affiliation: Department of the Navy, Regenerative Medicine, Naval Medical Research Center, Silver Spring, MD, USA.
authors.forename: F
authors.initials: F
authors.lastname: Gage
authors.forename: D B
authors.initials: DB
authors.lastname: Leeser
authors.forename: N K
authors.initials: NK
authors.lastname: Porterfield
authors.forename: J C
authors.initials: JC
authors.lastname: Graybill
authors.forename: S
authors.initials: S
authors.lastname: Gillern
authors.forename: J S
authors.initials: JS
authors.lastname: Hawksworth
authors.forename: R M
authors.initials: RM
authors.lastname: Jindal
authors.forename: N
authors.initials: N
authors.lastname: Thai
authors.forename: E M
authors.initials: EM
authors.lastname: Falta
authors.forename: D K
authors.initials: DK
authors.lastname: Tadaki
authors.forename: T S
authors.initials: TS
authors.lastname: Brown
authors.forename: E A
authors.initials: EA
authors.lastname: Elster
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D016030:Kidney Transplantation / Q000379:methods*; D023421:Models, Animal; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D016209:Interleukin-8; D019188:Organ Preservation Solutions; D014409:Tumor Necrosis Factor-alpha