Detection of multiple network-based allosteric interactions between peptides and arrays of DNA binding sites.
Quantitative DNase I footprinting shows that three designed peptides containing N-methylpyrrole (Py) moieties display different types of network-based allosteric communication in binding to DNA: circuit type, incomplete-circuit type, and non-circuit type characterized by interstrand bidentate interactions. Positive cooperative binding of all three peptides to individual DNA binding sites is commonly observed. CD spectral characterization of the interaction between peptides and model undecanucleotide duplexes is consistent with the footprinting results and supports the allosteric model. This study provides insights relating to the interaction network nature of allostery in complex DNA-small molecule interactions.
Kao
Karen K L
KK
Department of Chemistry and Life Science Center, Tunghai Christian University, Taichung 407, Taiwan, ROC.
Huang
Jonathan C T
JC
Yang
Chi-Kai
CK
Jeng
Kee-Ching G
KC
Chang
Jung-Cheng
JC
Yao
Wen-Chen
WC
Hsien
S C
SC
Waring
Michael J
MJ
Chen
Ming-Hua
MH
Ma
Lin
L
Sheh
Leung
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
D010455:Peptides; D004247:DNA; D003850:Deoxyribonuclease I