Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals for magnetically triggered delivery of siRNA.
Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals (PPMCs) were developed for magnetically triggered delivery of siRNA. The positively charged PPMCs formed stable nanosized polyelectrolyte complexes via electrostatic interactions with negatively charged siRNA-polyethylene glycol conjugate (siRNA-s-s-PEG) that was linked via a cleavable disulfide linkage. PPMC/siRNA-s-s-PEG polyelectrolyte complexes were efficiently taken up by cancer cells upon exposure to a magnet, thereby enhancing intracellular uptake and silencing effect of siRNA. The present study suggests that these novel nanomaterials could be potentially utilized for magnetically triggered delivery of various nucleic acid-based therapeutic agents.
Lee
Kyuri
K
Department of Biological Sciences and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
Bae
Ki Hyun
KH
Lee
Yuhan
Y
Lee
Soo Hyeon
SH
Ahn
Cheol-Hee
CH
Park
Tae Gwan
TG
D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D019793:Fluorescein; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008280:Magnetics / Q000379:methods*; D018613:Microscopy, Confocal; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D020442:Poloxamer / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000378:metabolism*
D003432:Cross-Linking Reagents; D053769:Nanocapsules; D034741:RNA, Small Interfering; D020442:Poloxamer; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D019793:Fluorescein; D052203:Ferrosoferric Oxide