title: Regulation of the cGMP-cPKG pathway and large-conductance Ca2+-activated K+ channels in uterine arteries during the ovine ovarian cycle.
abstract: The follicular phase of the ovine ovarian cycle demonstrates parallel increases in ovarian estrogens and uterine blood flow (UBF). Although estrogen and nitric oxide contribute to the rise in UBF, the signaling pathway remains unclear. We examined the relationship between the rise in UBF during the ovarian cycle of nonpregnant sheep and changes in the uterine vascular cGMP-dependent pathway and large-conductance Ca(2+)-activated K(+) channels (BK(Ca)). Nonpregnant ewes (n = 19) were synchronized to either follicular or luteal phase using a vaginal progesterone-releasing device (CIDR), followed by intramuscular PGF(2alpha), CIDR removal, and treatment with pregnant mare serum gonadotropin. UBF was measured with flow probes before tissue collection, and second-generation uterine artery segments were collected from nine follicular and seven luteal phase ewes. The pore-forming alpha- and regulatory beta-subunits that constitute the BK(Ca), soluble guanylyl cyclase (sGC), and cGMP-dependent protein kinase G (cPKG) isoforms (cPKG(1alpha) and cPKG(1beta)) were measured by Western analysis and cGMP levels by RIA. BK(Ca) subunits were localized by immunohistochemistry. UBF rose >3-fold (P < 0.04) in follicular phase ewes, paralleling a 2.3-fold rise in smooth muscle cGMP and 32% increase in cPKG(1alpha) (P < 0.05). sGC, cPKG(1beta), and the BK(Ca) alpha-subunit were unchanged. Notably, expression of beta(1)- and beta(2)-regulatory subunits rose 51 and 79% (P <or= 0.05), respectively. Increases in endogenous ovarian estrogens in follicular-phase ewes result in increases in UBF associated with upregulation of the cGMP- and cPKG-dependent pathway and increased vascular BK(Ca) beta/alpha-subunit stoichiometry, suggesting enhanced BK(Ca) activation contributes to the follicular phase rise in UBF.
authors.affiliation: Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical School, Dallas, Texas, USA.
authors.forename: Liaqat H
authors.initials: LH
authors.lastname: Khan
authors.forename: Charles R
authors.initials: CR
authors.lastname: Rosenfeld
authors.forename: Xiao-Tie
authors.initials: XT
authors.lastname: Liu
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Magness
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D030762:Estrous Cycle / Q000378:metabolism*; D005260:Female; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D006080:Ovarian Follicle / Q000254:growth & development; D010060:Ovulation / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology; D012756:Sheep; D015398:Signal Transduction / Q000502:physiology; D018709:Statistics, Nonparametric; D055988:Uterine Artery / Q000378:metabolism*; D014599:Uterus / Q000098:blood supply*
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D009569:Nitric Oxide; D017869:Cyclic GMP-Dependent Protein Kinases; D006152:Cyclic GMP