An in vitro study of the antioxidant activities and effect on human DNA of the Chinese herbal decoction 'Liu Wei Di Huang'.
A Chinese medicinal formulation, 'Liu Wei Di Huang', and its individual components have been tested for the genoprotective effect on human DNA by the comet assay. Results showed no DNA protection contributed by this prescription. However, the aqueous extracts of individual herbs, namely Cortex Moutan and Rhizoma Dioscoreae, were able to decrease by 10-15% the DNA stand break from hydrogen peroxide-mediated oxidative stress. This provides insight to further evaluate the interaction among herbs and the search for the active ingredient responsible for the DNA protective effect.
Szeto
Yim-Tong
YT
Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology Foundation, Avenida Wai Long, Taipa, Macau. ytszeto@must.edu.mo
Lei
Pui-Cheong
PC
Ngai
Kam-Leong
KL
Yiu
Andy Tak-Wah
AT
Chan
Catarina Sin-Pek
CS
Kok
Edith Wun-Fong
EW
Leong
Chi-Wa
CW
D000328:Adult; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D027742:Dioscorea; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D041743:Herb-Drug Interactions; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D062385:Hydroxybenzoates / Q000032:analysis; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D029596:Paeonia; D027343:Rhizome
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C042579:Liuwei Dihuang Decoction; D006861:Hydrogen Peroxide