BMAA neurotoxicity in Drosophila.
We report the establishment of an in vivo model using the fruit fly Drosophila melanogaster to investigate the toxic effects of L-BMAA. We found that dietary intake of BMAA reduced the lifespan as well as the neurological functions of flies. Furthermore, we have developed an HPLC method to reliably detect both free and protein-bound BMAA in fly tissue extracts.
Zhou
Xianchong
X
Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, Florida, USA.
Escala
Wilfredo
W
Papapetropoulos
Spyridon
S
Bradley
Walter G
WG
Zhai
R Grace
RG
D000409:Alanine / Q000494:pharmacology; D000599:Amino Acids, Diamino / Q000008:administration & dosage* / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000087522:Cyanobacteria Toxins; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D007930:Leucine / Q000627:therapeutic use; D008136:Longevity / Q000187:drug effects; D008239:Lysine / Q000627:therapeutic use; D008297:Male; D020258:Neurotoxicity Syndromes / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology*; D009498:Neurotoxins / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology
D000599:Amino Acids, Diamino; D000087522:Cyanobacteria Toxins; D009498:Neurotoxins; C001824:beta-N-methylamino-L-alanine; D018698:Glutamic Acid; D007930:Leucine; D008239:Lysine; D000409:Alanine