Histological intestinal alterations in chickens fed low protein diet.
To demonstrate whether that hypotrophied histological alterations of intestinal villi and epithelial cells are observed in chickens fed low crude protein (CP) diet, 36 male chickens were allotted into 10%, 16% and 22% CP diets groups for 35 days. The 10% CP group showed a lower value of weight gain and feed efficiency than the other two CP groups (p < 0.05). On the relative weight of visceral organs, the 10% CP group showed a heavier gizzard than the 22% CP group, a heavier jejunum than the other two CP groups and a heavier value of ileum and caeca than the 22% CP group (p < 0.05). On the relative length of intestines, the 10% CP group showed a longer value of duodenum and caeca than the other two CP groups, a longer jejunum than the 16% CP group and a longer colon than the 22% CP group (p < 0.05). The villus height and villus area of all intestinal segments did not change in all groups. The duodenal cell areas and cell mitosis numbers tended to be lower in 10% CP group than in the other two protein groups, and the jejunal cell area and duodenal cell mitosis numbers were decreased in the 10% crude protein group (p < 0.05). In relation to the protuberated cells in the higher CP groups, the 10% CP group showed only faintly protuberated cells on the duodenal and jejunal villus apical surface. The ileal villi did not show specific alterations among each group. The chronic feeding of low CP diet induced a hypotrophied histological alteration. This suggests that the hypotrophied histological alterations can assess that the fed-diet is not so well-balanced diets, nutritionally.
Buwjoom
T
T
Faculty of Animal Science and Technology, Maejo University, Chiang Mai, Thailand.
Yamauchi
K
K
Erikawa
T
T
Goto
H
H
D000818:Animals; D002645:Chickens / Q000033:anatomy & histology* / Q000502:physiology*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007413:Intestinal Mucosa / Q000187:drug effects / Q000648:ultrastructure; D007422:Intestines / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects; D008297:Male
D004044:Dietary Proteins