Imaging of gadolinium spatial distribution in tumor tissue by laser ablation inductively coupled plasma mass spectrometry.
OBJECTIVE
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized in postmortem imaging of gadolinium (Gd) spatial distribution in a mouse tumor model postadministration of PEGylated Gd liposomal nanoparticles.


METHODS
PEGylated liposomal nanoparticles were formulated using a paramagnetic lipid incorporating Gd, in addition to a fluorescent lipid, and injected intravenously into Balb/C nude mice bearing IGROV-1 tumors. At postinjection (2 h), the tumors and selective organs were imaged by magnetic resonance imaging (MRI) and, after excision, by histology and LA-ICP-MS.


RESULTS
The presence of Gd within tumor tissue was confirmed by LA-ICP-MS and when correlated to histology was found to be prevalent in regions of higher vascularity. The presence of Gd in the kidneys was also confirmed.


CONCLUSIONS
We have demonstrated, in a novel manner, the use of LA-ICP-MS for the spatial detection of Gd in tumor tissue. LA-ICP-MS is valuable in providing spatio-specific information of MRI contrast agents and more importantly Gd in tumor tissue.
Kamaly
Nazila
N
Imaging Sciences Department, Metabolic and Molecular Imaging Group, MRC Clinical Sciences Center, Hammersmith Hospital, Imperial College London, London W120NN, UK.
Pugh
John A
JA
Kalber
Tammy L
TL
Bunch
Josephine
J
Miller
Andrew D
AD
McLeod
Cameron W
CW
Bell
Jimmy D
JD
D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005682:Gadolinium / Q000493:pharmacokinetics*; D006801:Humans; D007668:Kidney / Q000473:pathology; D053685:Laser Therapy / Q000379:methods*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D013054:Spectrophotometry, Atomic / Q000379:methods*
D005682:Gadolinium