title: Electrochemical study of functionalization on the surface of a chitin/platinum-modified glassy carbon paste electrode.
abstract: To functionalize chitin surfaces using proteins, we developed a glucose oxidase (GOD)-chitin/platinum-modified glassy carbon paste electrode (GCPE) as a model. In a weakly acidic solution, negatively charged GOD were immobilized by the protonated acetylamide groups on chitin. When the electrode was immersed in a solution containing GOD, the enzyme was readily immobilized due to the electrostatic interaction. In addition, measurements were performed using electrodes made with powders of different sizes because sensor performance depends on the particle sizes of glassy carbon powder.
authors.affiliation: Faculty of Engineering, Maebashi Institute of Technology, Maebashi, Gunma 371-0816, Japan. kzsuga@maebashi-it.ac.jp
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Sugawara
authors.forename: Asako
authors.initials: A
authors.lastname: Yugami
authors.forename: Norifumi
authors.initials: N
authors.lastname: Terui
authors.forename: Hideki
authors.initials: H
authors.lastname: Kuramitz
mesh_terms: D000577:Amides / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D002686:Chitin / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D010316:Particle Size; D010984:Platinum / Q000737:chemistry*; D011522:Protons; D013499:Surface Properties
chemical_list: D000577:Amides; D004800:Enzymes, Immobilized; D011522:Protons; D002686:Chitin; D010984:Platinum; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose