title: Structural insights into replication initiation and elongation processes by the FMDV RNA-dependent RNA polymerase.
abstract: RNA-dependent RNA polymerases (RdRPs) play central roles in both transcription and viral genome replication. In picornaviruses, these functions are catalyzed by the virally encoded RdRP, termed 3D. Polymerase 3D also catalyzes the covalent linkage of UMP to a tyrosine on the small protein VPg. Uridylylated VPg then serves as a protein primer for the initiation of RNA synthesis. Seven different crystal structures of foot-and-mouth disease virus (FMDV) 3D catalytic complexes have enhanced our understanding of template and primer recognition, VPg uridylylation, and rNTP binding and catalysis. Such structural information is providing new insights into the fidelity of RNA replication, and for the design of antiviral compounds.
authors.affiliation: Institut de Biologia Molecular de Barcelona (CSIC), Parc Científic de Barcelona, Baldiri i Reixac 10, E-08028 Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ferrer-Orta
authors.forename: Rubén
authors.initials: R
authors.lastname: Agudo
authors.forename: Esteban
authors.initials: E
authors.lastname: Domingo
authors.forename: Nuria
authors.initials: N
authors.lastname: Verdaguer
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D029544:Foot-and-Mouth Disease Virus / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase