title: The stress response protein Gls24 is induced by copper and interacts with the CopZ copper chaperone of Enterococcus hirae.
abstract: Intracellular copper routing in Enterococcus hirae is accomplished by the CopZ copper chaperone. Under copper stress, CopZ donates Cu(+) to the CopY repressor, thereby releasing its bound zinc and abolishing repressor-DNA interaction. This in turn induces the expression of the cop operon, which encodes CopY and CopZ, in addition to two copper ATPases, CopA and CopB. To gain further insight into the function of CopZ, the yeast two-hybrid system was used to screen for proteins interacting with the copper chaperone. This led to the identification of Gls24, a member of a family of stress response proteins. Gls24 is part of an operon containing eight genes. The operon was induced by a range of stress conditions, but most notably by copper. Gls24 was overexpressed and purified, and was shown by surface plasmon resonance analysis to also interact with CopZ in vitro. Circular dichroism measurements revealed that Gls24 is partially unstructured. The current findings establish a novel link between Gls24 and copper homeostasis.
authors.affiliation: Department of Clinical Pharmacology and Visceral Research, University of Berne, Berne, Switzerland.
authors.forename: Jivko V
authors.initials: JV
authors.lastname: Stoyanov
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancini
authors.forename: Zen Huat
authors.initials: ZH
authors.lastname: Lu
authors.forename: Frédéric
authors.initials: F
authors.lastname: Mourlane
authors.forename: Kristian R
authors.initials: KR
authors.lastname: Poulsen
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.forename: Marc
authors.initials: M
authors.lastname: Solioz
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D016983:Enterococcus / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006706:Homeostasis; D018832:Molecular Chaperones / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon; D013312:Stress, Physiological; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; C092379:CopZ protein, Enterococcus hirae; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D015534:Trans-Activators; D003300:Copper