title: [Arteriogenesis induced by intramyocardial recombinant adeno-associated virus vector encoding human CD151 cDNA gene transfer in swines with coronary artery occlusion].
abstract: OBJECTIVE
  To investigate the efficacy of CD151 gene delivery in promoting blood perfusion in swines after myocardial infarction.
  
  
  METHODS
  Swines received coronary artery ligation and intramyocardial injection with rAAV-CD151, rAAV-anti-CD151 or rAAV-GFP. Eight weeks after vector injection, Western blot, immunostaining and 13N-labeled NH3 PET were performed to detect gene expression and biological effects of various treatments.
  
  
  RESULTS
  High level of CD151 protein expression was detected in the rAAV-CD151 group. The capillary density in the rAAV-CD151 group [(83.8 +/- 6.7) n/mm2] was significantly higher than that in the control group [(33.2 +/- 4.5) n/mm2] and rAAV-GFP group [(41.6 +/- 5.6) n/mm2] (all P<0.05); the arteriole density in the rAAV-CD151 group [(16.4 +/- 2.5) n/mm2] was also higher than that in the control group [(6.6 +/- 2.3) n/mm2] and the rAAV-GFP group [(8.4 +/- 1.6) n/mm2] (all P<0.05). However, the lowest capillary density and arteriole density were evidenced in rAAV-anti-CD151 group. Myocardial blood perfusion was significantly increased in rAAV-CD151 group and significantly reduced in rAAV-anti-CD151 group (all P<0.05 vs. control).
  
  
  CONCLUSIONS
  Intramyocardial injection of rAAV-CD151 could enhance the myocardial express of CD151 protein, increase capillary and arteriole densities and improve blood perfusion in swine with myocardial infarction.
authors.affiliation: Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hou-juan
authors.initials: HJ
authors.lastname: Zuo
authors.forename: Zheng-xiang
authors.initials: ZX
authors.lastname: Liu
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Liu
authors.forename: He-song
authors.initials: HS
authors.lastname: Zeng
authors.forename: Sha
authors.initials: S
authors.lastname: Wen
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Dao-wen
authors.initials: DW
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D054059:Coronary Occlusion / Q000628:therapy*; D000229:Dependovirus / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000628:therapy*; D018919:Neovascularization, Physiologic; D013552:Swine; D013556:Swine, Miniature; D060185:Tetraspanin 24; D016896:Treatment Outcome
chemical_list: D015703:Antigens, CD; C554715:CD151 protein, human; D060185:Tetraspanin 24