title: Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin.
abstract: Pogostemon cablin (PC) is a herbal medicine traditionally applied to treat not only common cold, nausea and diarrhea but also headache and fever. The aim of this study was to investigate the analgesic and anti-inflammatory properties of standardized PC methanol extract (PCMeOH) in vivo. Investigations were performed in mice with two analgesic models. One was acetic acid-induced writhing response and the other formalin-induced paw licking. The anti-inflammatory effect was tested by λ-carrageenan (Carr)-induced mice paw edema. These analgesic experimental results indicated that PCMeOH (1.0 g/kg) decreased the acetic acid-induced writhing responses and PCMeOH (0.5 and 1.0 g/kg) decreased the licking time in the second phase of the formalin test. Moreover, Carr-induced paw edema inflammation was significantly reduced in a dose-dependent manner when PCMeOH (0.5 and 1.0 g/kg) was administered 3 and 4 h after the Carr injection. Mechanistic studies showed that PCMeOH decreased the levels of malondialdehyde in the edema paw by increasing the activities of anti-oxidant enzymes, such as superoxide dismutase, glutathione peroxidase and glutathione reductase, in the liver and decreasing the cyclooxygenase 2 and tumor necrosis factor-α activities in the edema paw. This study has demonstrated the analgesic and anti-inflammatory effects of PCMeOH, thus verifying its popular use in traditional medicine.
authors.affiliation: Graduate Institute of Chinese Pharmaceutical Sciences, College of Pharmacy, China Medical University, 91, Hsieh Shih Road, Taichung, Taiwan.
authors.forename: Tsung-Chun
authors.initials: TC
authors.lastname: Lu
authors.forename: Jung-Chun
authors.initials: JC
authors.lastname: Liao
authors.forename: Tai-Hung
authors.initials: TH
authors.lastname: Huang
authors.forename: Ying-Chih
authors.initials: YC
authors.lastname: Lin
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Liu
authors.forename: Yung-Jia
authors.initials: YJ
authors.lastname: Chiu
authors.forename: Wen-Huang
authors.initials: WH
authors.lastname: Peng