title: Bacterial leakage evaluation of root canals filled with different endodontic sealers.
abstract: OBJECTIVE
  The objective of this study was to evaluate the sealing ability of AH Plus, Epiphany, Acroseal, Endofill, and Polifil after active lateral condensation technique, by using a bacterial test, during 64 days.
  
  
  METHODS
  One hundred bovine incisors were cleaned and shaped; then they were filled with the endodontic sealers and adapted into a microcentrifuge tube. The setup root/microcentrifuge tube was added to glass flasks containing Brain Heart Infusion broth. A culture of Enterococcus faecalis was inserted into the upper chamber of each assembly. Daily leakage was evaluated through the broth turbidity.
  
  
  RESULTS
  The results were submitted to statistical analysis (Kaplan-Meier method, Kruskal-Wallis and Dunn tests).
  
  
  CONCLUSIONS
  AH Plus and Endofill had the worst sealing ability when compared with Polifil, which showed the least leakage. Acroseal and Epiphany showed a tendency toward having an intermediate behavior; however, there was no significant difference among Acroseal, Epiphany, and the other sealers.
authors.affiliation: Department of Restorative Dentistry, Araraquara Dental School, São Paulo State University, Araraquara, São Paulo, Brazil. claudiaendo@gmail.com
authors.forename: Cláudia Ramos
authors.initials: CR
authors.lastname: Pinheiro
authors.forename: Adriana Simionatto
authors.initials: AS
authors.lastname: Guinesi
authors.forename: Ericson Janólio
authors.initials: EJ
authors.lastname: de Camargo
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Pizzolitto
authors.forename: Idomeo Bonetti
authors.initials: IB
authors.lastname: Filho
mesh_terms: D000818:Animals; D002417:Cattle; D003763:Dental Leakage / Q000517:prevention & control*; D018772:Dental Marginal Adaptation; D003786:Dental Pulp Cavity / Q000382:microbiology / Q000601:surgery; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004853:Epoxy Resins / Q000737:chemistry / Q000145:classification; D007180:Incisor; D008422:Materials Testing; D012387:Root Canal Filling Materials / Q000737:chemistry* / Q000145:classification; D012389:Root Canal Obturation / Q000379:methods*; D018709:Statistics, Nonparametric
chemical_list: D004853:Epoxy Resins; D012387:Root Canal Filling Materials