title: Differential effects of substance P or hemokinin-1 on transient receptor potential channels, TRPV1, TRPA1 and TRPM8, in the rat.
abstract: Two tachykinin peptides, substance P (SP) and hemokinin-1 (HK-1), and three transient receptor potential (TRP) channels, TRPV1, TRPA1 and TRPM8, are similarly localized in the spinal dorsal horn and dorsal root ganglion, suggesting that TRP channels may be related or modulated by these tachykinin peptides. Thus, to clarify whether the responses of TRP channels are modulated by SP or HK-1, the effects of pretreatment with SP or HK-1 on the induction of scratching behavior by TRP channel agonists were examined. Pretreatment with SP or HK-1 enhanced the induction of scratching behavior by resiniferatoxin, a TRPV1 agonist, whereas scratching behavior induced by menthol, a TRPM8 agonist, was suppressed by pretreatment with these peptides. On the other hand, pretreatment with SP, but not HK-1, suppressed the induction of scratching behavior by cinnamaldehyde, a TRPA1 agonist. Taken together, the present results indicate that SP or HK-1 differentially modulated the response of TRPV1, TRPA1 or TRPM8 channel.
authors.affiliation: Division of Neurobiology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. rumi.n@fc.miyazaki-u.ac.jp
authors.forename: Rumi
authors.initials: R
authors.lastname: Naono-Nakayama
authors.forename: Natsuki
authors.initials: N
authors.lastname: Sunakawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikeda
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Nishimori
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D017487:Ankyrins; D001522:Behavior, Animal / Q000187:drug effects; D015220:Calcium Channels / Q000096:biosynthesis* / Q000235:genetics; D004224:Diterpenes / Q000494:pharmacology; D007278:Injections, Spinal; D008297:Male; D008610:Menthol / Q000494:pharmacology; D011537:Pruritus / Q000139:chemically induced / Q000523:psychology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology*; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D050053:TRPM Cation Channels / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D050916:TRPV Cation Channels / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D015320:Tachykinins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D017487:Ankyrins; D015220:Calcium Channels; D004224:Diterpenes; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; D015320:Tachykinins; C480976:Trpa1 protein, rat; C498947:Trpm8 protein, rat; C493922:Trpv1 protein, rat; C507968:hemokinin-1; D008610:Menthol; D013373:Substance P; D000171:Acrolein; C024353:resiniferatoxin; C012843:cinnamaldehyde