title: Comparison of a novel confocal scanning laser ophthalmoscopy algorithm with optical coherence tomography in measurement of macular thickness and volume.
abstract: OBJECTIVE
  To evaluate macular thickness and volume measurements using a novel Heidelberg Retinal Tomograph (HRT3) algorithm and to compare the results with optical coherence tomography (StratusOCT).
  
  
  METHODS
  This was a prospective, comparative, clinical noninterventional study. Fifty-two eyes were examined using both methods: 16 normal eyes, 12 eyes with diabetic macular edema (DME), and 24 eyes with age-related macular degeneration (AMD). The HRT3 macular thickness and volume were compared with those from the StratusOCT automated software. In addition, the distances between the retinal surface and the base of subretinal pathologic changes were measured using the StratusOCT manual-caliper technique and were compared with the HRT3 measurements in AMD eyes.
  
  
  RESULTS
  No statistically significant difference in macular thickness and volume measurements was observed between the two methods for normal (P = 0.89 and P = 0.33, respectively) and DME eyes (P = 0.86 and P = 0.31, respectively). However, in AMD eyes, the differences in both measurements were statistically significant between the two methods (P < 0.0001 and P < 0.0001, respectively). The HRT3 thickness measurements were in good agreement with the manual StratusOCT measurements (P = 0.4).
  
  
  CONCLUSIONS
  In normal and DME eyes, the HRT3 measurements correlated well with the automatic StratusOCT measurements. In AMD eyes, the HRT3 measurements correlated well only with the manually corrected measurements of the retinal-subretinal pathologic changes.
authors.affiliation: Jacobs Retina Center, University of California San Diego, Shiley Eye Center, La Jolla, California 92037, USA.
authors.forename: Byung Ro
authors.initials: BR
authors.lastname: Lee
authors.forename: Dirk-Uwe
authors.initials: DU
authors.lastname: Bartsch
authors.forename: Igor
authors.initials: I
authors.lastname: Kozak
authors.forename: Lingyun
authors.initials: L
authors.lastname: Cheng
authors.forename: William R
authors.initials: WR
authors.lastname: Freeman
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D005260:Female; D006801:Humans; D007834:Lasers; D008266:Macula Lutea / Q000473:pathology*; D008268:Macular Degeneration / Q000175:diagnosis*; D008269:Macular Edema / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009887:Ophthalmoscopy / Q000379:methods*; D011446:Prospective Studies; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000379:methods*