title: Suppression of type I collagen production by microRNA-29b in cultured human stellate cells.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression through imperfect base pairing with the 3' untranslated region (3'UTR) of target mRNA. We studied the regulation of alpha 1 (I) collagen (Col1A1) expression by miRNAs in human stellate cells, which are involved in liver fibrogenesis. Among miR-29b, -143, and -218, whose expressions were altered in response to transforming growth factor-beta1 or interferon-alpha stimulation, miR-29b was the most effective suppressor of type I collagen at the mRNA and protein level via its direct binding to Col1A1 3'UTR. miR-29b also had an effect on SP1 expression. These results suggested that miR-29b is involved in the regulation of type I collagen expression by interferon-alpha in hepatic stellate cells. It is anticipated that miR-29b will be used for the regulation of stellate cell activation and lead to antifibrotic therapy.
authors.affiliation: Department of Hepatology, Graduate School of Medicine, Osaka City University, Osaka, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ogawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Iizuka
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Sekiya
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ikeda
authors.forename: Norifumi
authors.initials: N
authors.lastname: Kawada
mesh_terms: D020413:3' Untranslated Regions; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D005786:Gene Expression Regulation; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016898:Interferon-alpha; C542359:MIRN143 microRNA, human; C547471:MIRN218 microRNA, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D016329:Sp1 Transcription Factor