title: Functional effects of antibodies against non-neuronal nicotinic acetylcholine receptors.
abstract: Non-neuronal nicotinic acetylcholine receptors (nAChRs) are expressed in the spleen and regulate B lymphocyte propagation and activation. The aim of the present study was to investigate the cellular and physiological effects of antibodies against alpha4(1-209) and alpha7(1-208) nAChR extracellular domains. The antibodies, added in vitro, produced in vivo or injected, specifically bound mouse spleen B lymphocytes. Immunization with nAChR extracellular domains resulted in connective tissue overgrowth and infiltration of segmented neutrophils in the spleen, as well as in decreased body weight compared to mice immunized with BSA. In spite of certain cross-reactivity of alpha4(1-209)- and alpha7(1-208)-specific antibodies, all observed effects were more pronounced upon immunization with alpha7 extracellular domain. Spleens of mice injected with alpha7(1-208)-specific antibody contained decreased numbers of Annexin V-positive B lymphocytes compared to mice injected with non-specific IgG. It is concluded that alpha7 nAChRs are involved in regulating the lymphocyte survival, neutrophil migration, connective tissue overgrowth and body weight accumulation. The antibody binding triggers alpha7 nAChR signaling supporting the idea of non-channel mode of nAChR functioning in B lymphocytes.
authors.affiliation: Palladin Institute of Biochemistry, 9, Leontovicha str., 01601 Kyiv, Ukraine.
authors.forename: Olena
authors.initials: O
authors.lastname: Lykhmus
authors.forename: Lyudmyla
authors.initials: L
authors.lastname: Koval
authors.forename: Svitlana
authors.initials: S
authors.lastname: Pavlovych
authors.forename: Marios
authors.initials: M
authors.lastname: Zouridakis
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Zisimopoulou
authors.forename: Socrates
authors.initials: S
authors.lastname: Tzartos
authors.forename: Victor
authors.initials: V
authors.lastname: Tsetlin
authors.forename: Olga
authors.initials: O
authors.lastname: Volpina
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cloëz-Tayarani
authors.forename: Sergiy
authors.initials: S
authors.lastname: Komisarenko
authors.forename: Maryna
authors.initials: M
authors.lastname: Skok
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D017209:Apoptosis; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D007114:Immunization; D007116:Immunization, Passive; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011817:Rabbits; D011978:Receptors, Nicotinic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000378:metabolism* / Q000473:pathology
chemical_list: D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D011978:Receptors, Nicotinic