A label-free strategy for facile electrochemical analysis of dynamic glycan expression on living cells.
A novel, label-free strategy has been developed for facile electrochemical analysis of dynamic glycan expression on living cells, which uses carbon nanohorns to efficiently immobilize lectin for the construction of a recognition interface and enhancing accessibility of cell surface glycan motifs.
Ding
Lin
L
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
Cheng
Wei
W
Wang
Xiaojian
X
Xue
Yadong
Y
Lei
Jianping
J
Yin
Yibing
Y
Ju
Huangxian
H
D002244:Carbon; D002477:Cells; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D020014:K562 Cells; D037102:Lectins / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000235:genetics
D055767:Immobilized Proteins; D037102:Lectins; D011134:Polysaccharides; D002244:Carbon