title: Fast counter current chromatography of n-butanolic fraction from Senecio giganteus (Asteraceae).
abstract: Phenolic derivatives such as quinones, acid-phenols and flavonoids were successfully isolated from a n-butanolic fraction of Senecio giganteus Desf. (Asteraceae) flowers, namely jacaranone (1), 3a-hydroxy-3,3a,7,7a-tetrahydrobenzofuran-2,6-dione (2), chlorogenic acid (3), hyperoside (4), quercetin 3-O-beta-D-robinobioside (5), isorhamnetin-3-O-beta-D-glucuronide (6), quercetin-3-O-beta-D-glucuronide (7), and isorhamnetin-3-O-beta-D-glucuronide-6"-methyl ester (8). These compounds were purified through either classical polyamide filtration followed by fractionation on Si gel, or through fast centrifugal partition chromatography (FCPC). Using FCPC, the major compounds could be readily isolated from the crude n-butanolic fraction. Compounds 1-8 were identified by means of spectroscopic and spectrometric analysis (UV, 1H, 13C and 2D NMR, and MS). This work described for the first time the phytochemical composition of this endemic Algerian plant.
authors.affiliation: Université Mentouri, Constantine, laboratoire de Phytochimie et d'Analyses Physico-chimiques et Biologiques, département de Chimie, Faculté des Sciences exactes, Algérie.
authors.forename: Nadjet
authors.initials: N
authors.lastname: Mezache
authors.forename: Séverine
authors.initials: S
authors.lastname: Derbré
authors.forename: Salah
authors.initials: S
authors.lastname: Akkal
authors.forename: Hocine
authors.initials: H
authors.lastname: Laouer
authors.forename: Denis
authors.initials: D
authors.lastname: Séraphin
authors.forename: Pascal
authors.initials: P
authors.lastname: Richomme
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D015394:Molecular Structure; D012674:Senecio / Q000737:chemistry*
chemical_list: D020001:1-Butanol