title: Isolation and identification of urinary metabolites of kakkalide in rats.
abstract: Kakkalide is a major isoflavonoid from the flowers of Pueraria lobata (Willd.) Ohwi, possessing the protective effect against ethanol-induced intoxication and hepatic injury. The metabolism of kakkalide was investigated in rats. Thirteen metabolites were isolated by using solvent extraction and repeated chromatographic methods and identified by using spectroscopic methods including UV, IR, mass spectrometry, NMR, and circular dichroism experiments. Four new compounds were identified as irisolidone-7-O-glucuronide (M-1), tectorigenin-7-O-sulfate (M-2), tectorigenin-4'-O-sulfate (M-3), and biochanin A-6-O-sulfate (M-4) together with nine known compounds identified as irisolidone (M-5), tectorigenin (M-6), tectoridin (M-7), 5,7-dihydroxy-8,4'-dimethoxyisoflavone (M-8), isotectorigenin (M-9), biochanin A (M-10), genistein (M-11), daidzein (M-12), and equol (M-13). The metabolic pathway of kakkalide was proposed, which is important to understand its metabolic fate and disposition in humans.
authors.affiliation: Department of Traditional Chinese Medicines, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jialin
authors.initials: J
authors.lastname: Qu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kano
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000652:urine; D035264:Flowers / Q000737:chemistry; D020719:Glucuronides / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D006027:Glycosides / Q000302:isolation & purification / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine; D008658:Inactivation, Metabolic; D007529:Isoflavones / Q000302:isolation & purification / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D020719:Glucuronides; D006027:Glycosides; D007529:Isoflavones; D013463:Sulfuric Acid Esters; C108698:kakkalide