Bronchorelaxant property of 'African potato' (Hypoxis hemerocallidea corm) aqueous extract in vitro.
This study was undertaken to investigate the bronchorelaxant effect of Hypoxis hemerocallidea corm ('African potato') aqueous extract (APE) on spasmogen-provoked contractions of guinea-pig isolated tracheal smooth muscle preparations. APE (25-400 mg/ml) relaxed spasmogen (histamine-, carbachol- and potassium-)-induced contractions of the isolated tracheal muscle preparations in a concentration-dependent manner. The relaxant effects of APE on spasmogen-evoked contractions of the tracheal muscle preparations were not altered by bath-applied propranolol (0.1-5.0 microg/ml), which markedly inhibited or completely abolished the relaxant effects of isoprenaline (0.1-5.0 microg/ml). Although the precise mechanism of the bronchorelaxant effect of APE could not be established in the present study, it is unlikely that the herb's aqueous extract stimulates the beta(2)-adrenoceptors present on the bronchial smooth muscles to produce its bronchodilatation. The finding that APE significantly relaxed (P<0.05) histamine-, carbachol- and high potassium ion concentration (K(+), 80 mM)-induced contractions of guinea-pig isolated bronchial muscle preparations appears to suggest that the bronchospasmolytic effect of the plant's extract is probably not mediated through a specific receptor, but rather, probably mediated via a non-specific bronchospasmolytic mechanism.
Ojewole
John A O
JA
Department of Pharmacology, School of Pharmacy & Pharmacology, Faculty of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa. ojewolej@ukzn.ac.za
Olayiwola
Gbola
G
Nyinawumuntu
Agatha
A
D000818:Animals; D001980:Bronchi / Q000187:drug effects*; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D027802:Hypoxis / Q000737:chemistry*; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D008297:Male; D016488:Medicine, African Traditional; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects / Q000502:physiology
D010936:Plant Extracts; D006632:Histamine; D002217:Carbachol