title: Calculation errors of time-varying flux control coefficients obtained from elasticity coefficients by means of summation and connectivity theorems in metabolic control analysis.
abstract: This paper investigates the accuracy of a matrix method proposed by other researchers to calculate time-varying flux control coefficients (dynamic FCCs) from elasticity coefficients by means of summation and connectivity theorems in the framework of metabolic control analysis. A mathematical model for the fed-batch penicillin V fermentation process is used as a case example for discussion. Calculated results reveal that this method produces significant calculation errors because the theorems are essentially valid only in steady state, although it may provide rough time-transient behaviors of FCCs. Strictly, therefore, dynamic FCCs should be directly calculated from the differential equations for metabolite concentrations and sensitivities.
authors.affiliation: Department of Systems Design, Bio-Architecture Center, Kyushu University, Hakozaki, Fukuoka, Japan. fumishira@brs.kyushu-u.ac.jp
authors.forename: Fumihide
authors.initials: F
authors.lastname: Shiraishi
authors.forename: Kansuporn
authors.initials: K
authors.lastname: Sriyudthsak
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D019149:Bioreactors; D005285:Fermentation / Q000502:physiology*; D007700:Kinetics; D008954:Models, Biological; D010404:Penicillin V / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*
chemical_list: D010404:Penicillin V