title: Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum.
abstract: In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.
authors.affiliation: Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK.
authors.forename: Zisis
authors.initials: Z
authors.lastname: Kozlakidis
authors.forename: Neil A
authors.initials: NA
authors.lastname: Brown
authors.forename: Atif
authors.initials: A
authors.lastname: Jamal
authors.forename: Xiyu
authors.initials: X
authors.lastname: Phoon
authors.forename: Robert H A
authors.initials: RH
authors.lastname: Coutts
mesh_terms: D010802:Phylogeny; D010838:Phytophthora / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*