Development of rapid, automated diagnostics for infectious disease: advances and challenges.
The last 2 years has seen an exponential rise in the amount of research funding made available for the development of rapid diagnostic devices for infectious agents of medical importance. This review reports on several such projects. These highlight the development of fully automated devices for rapid diagnostics, ranging from fully automated real-time PCR-based detection methods to fully automated PCR- and array-based machines for the detection and typing of influenza. This review will also highlight the importance of refocusing work on classical immunoassay techniques, showing how biosensor-based immunoassays can greatly enhance existing assays and at a much reduced cost to molecular-based methods.
Ince
Jennifer
J
School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG118NS, UK.
McNally
Alan
A
D001331:Automation; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D003141:Communicable Diseases / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers