An approach of the laboratory to the field: assessment of the influence of cattle management on the seroprevalence of fascioliasis by using polyclonal- and recombinant-based ELISAs.
A cross-sectional study was conducted to assess the seroprevalence of fascioliasis by immunoenzymatic probes in an endemic area (northwestern Spain). Blood samples were collected from 1,034 cattle (crossbred, Rubia Gallega, and Friesian breeds), and the diagnosis of fascioliasis was carried out by determining both the occurrence of antigenemia and the presence of specific IgG antibodies against a Fasciola hepatica recombinant protein (FhrAPS). The IgG seroprevalence was 65% (95% CI, 62-68) by the FhrAPS-ELISA, and 32% (29-35) exhibited antigenemia; the lowest percentages occurred in the Friesians, and the highest percentages were found in the crossbreds. These results confirm an elevated seroprevalence of fascioliasis that is unexpected considering that most of the cattle livestock (Friesian and Rubia Gallega) receive fasciolicide treatment. The lack of adequate measures on the environment and erratic chemotherapy seem to be responsible for the fact that control of fascioliasis has not improved in the last 10 yr in the area of study.
Arias
M
M
Animal Pathology Department, Veterinary College, University of Santiago de Compostela, 27002-Lugo, Spain. adolfo.paz@usc.es
Piñeiro
P
P
Hillyer
G V
GV
Suárez
J L
JL
Francisco
I
I
Cortiñas
F J
FJ
Díez-Baños
P
P
Morrondo
P
P
Sánchez-Andrade
R
R
Paz-Silva
A
A
D000367:Age Factors; D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D003430:Cross-Sectional Studies; D019353:Endemic Diseases / Q000706:statistics & numerical data / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000453:epidemiology / Q000662:veterinary*; D005260:Female; D015801:Helminth Proteins / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies; D013030:Spain / Q000453:epidemiology
D000871:Anthelmintics; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins