Detection of occult Scedosporium species in respiratory tract specimens from patients with cystic fibrosis by use of selective media.
Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and Scedosporium-selective medium (SceSel+). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a Scedosporium sp., most frequently Scedosporium aurantiacum (17/218). Scedosporium was recovered on SceSel+, Mycosel, and SABD from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.
Blyth
C C
CC
Centre of Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Darcy Rd., Westmead, NSW 2145, Australia. ccblyth@gmail.com
Harun
A
A
Middleton
P G
PG
Sleiman
S
S
Lee
O
O
Sorrell
T C
TC
Meyer
W
W
Chen
S C A
SC
D003470:Culture Media / Q000737:chemistry; D003550:Cystic Fibrosis / Q000150:complications*; D006801:Humans; D009172:Mycology / Q000379:methods; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D012137:Respiratory System / Q000382:microbiology*; D021681:Scedosporium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media