title: Heparan sulfate separation, sequencing, and isomeric differentiation: ion mobility spectrometry reveals specific iduronic and glucuronic acid-containing hexasaccharides.
abstract: We describe the resolution of heparan sulfate (HS) isomers by chromatographic methods and their subsequent differentiation by mass spectrometry (MS), ion mobility, and (1)H nuclear magnetic resonance (NMR) analysis. The two purified hexasaccharide isomers produced nearly identical MS spectra, quantitative disaccharide profiles, and partial enzymatic digestions. However, both tandem spectrometry (MS(2)) and ion mobility spectrometry (IMS) indicated structural differences existed. All data suggested the distinction between the two hexasaccharides resided in their uronic acid stereochemistries. Glucuronic (GlcA) and iduronic acids (IdoA) were subsequently defined by (1)H NMR analysis completing the structural analysis and verifying the unique structures initially indicated by MS(2) and IMS. Our results suggest that IMS may be a powerful tool in the rapid differentiation of GlcA and IdoA containing isomers in the absence of prior structural knowledge.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Schenauer
authors.forename: John K
authors.initials: JK
authors.lastname: Meissen
authors.forename: Youjin
authors.initials: Y
authors.lastname: Seo
authors.forename: James B
authors.initials: JB
authors.lastname: Ames
authors.forename: Julie A
authors.initials: JA
authors.lastname: Leary
mesh_terms: D002240:Carbohydrate Sequence; D020723:Glucuronic Acid / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry / Q000302:isolation & purification*; D007067:Iduronic Acid / Q000737:chemistry*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D020723:Glucuronic Acid; D006497:Heparan Sulfate; D007067:Iduronic Acid; D009844:Oligosaccharides