A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
Various dendritic cell (DC) populations exist that differ in phenotype and ability to present antigen to T cells. For example, plasmacytoid DCs (pDCs) are less potent T cell activators compared with conventional DCs (cDCs). Here, we compared porcine blood DCs (BDCs), containing pDCs and cDCs, and monocyte-derived DCs (MoDC), consisting of cDCs, in their phenotype, ability to uptake antigen, activation and maturation and their ability to present antigen to autologous T cells. Pigs represent an important animal model, whose immune system in many respects closely resembles that of humans. For example, the distribution of Toll-like receptors is similar to that of humans, in contrast to that of mice. Here we demonstrate that both populations endocytose foreign material. Following lipopolysaccharide stimulation, CD80/86 and chemokine receptor (CCR)7 expression was increased in both populations as was the expression of the chemokine ligands (CCL)-2, CCL-4, CCL-20 and CXCL-2. Although basal and post-stimulation protein concentrations of interleukins 6 and 8 and tumour necrosis factor-alpha were higher in MoDCs, protein concentrations showed a higher fold increase in BDCs. Antigen-specific proliferation of autologous T cells was induced by MoDCs and BDCs. Interestingly, while MoDCs induced stronger proliferation in naive T cells, no difference in proliferation was observed when primed T cells were studied. These results demonstrate that isolated porcine BDCs are highly responsive to stimulation with lipopolysaccharide and are functionally able to drive primed T-cell proliferation to the same extent as MoDCs.
Facci
Marina R
MR
Vaccine & Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK.
Auray
Gael
G
Buchanan
Rachelle
R
van Kessel
Jill
J
Thompson
David R
DR
Mackenzie-Dyck
Sarah
S
Babiuk
Lorne A
LA
Gerdts
Volker
V
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D018925:Chemokines / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000276:immunology; D004705:Endocytosis / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D015870:Gene Expression / Q000187:drug effects; D016130:Immunophenotyping; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology*; D010047:Ovalbumin / Q000276:immunology; D054400:Receptors, CCR7 / Q000235:genetics; D034421:Sus scrofa; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; C515370:CCR7 protein, human; D018925:Chemokines; D016207:Cytokines; D003911:Dextrans; D008070:Lipopolysaccharides; D054400:Receptors, CCR7; C015219:fluorescein isothiocyanate dextran; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate