Tc17 CD8 T cells: functional plasticity and subset diversity.
IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-gamma and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-gamma-positive or IL-17/IFN-gamma-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-gamma-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-gamma-producing cells are desired.
Yen
Hung-Rong
HR
Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
Harris
Timothy J
TJ
Wada
Satoshi
S
Grosso
Joseph F
JF
Getnet
Derese
D
Goldberg
Monica V
MV
Liang
Kai-Li
KL
Bruno
Tullia C
TC
Pyle
Kristin J
KJ
Chan
Siaw-Li
SL
Anders
Robert A
RA
Trimble
Cornelia L
CL
Adler
Adam J
AJ
Lin
Tzou-Yien
TY
Pardoll
Drew M
DM
Huang
Ching-Tai
CT
Drake
Charles G
CG
D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D020381:Interleukin-17; D050796:STAT3 Transcription Factor; D007371:Interferon-gamma