Comparative validation study to demonstrate the equivalence of a minor modification to AOAC Method 997.03 Visual Immunoprecipitate (VIP) for Listeria to the reference culture method.
The Visual Immunoprecipitate (VIP) for the Detection of Listeria in Foods and Environmental Surfaces, AOAC Official Method 997.03, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Two food matrixes and one environmental surface were analyzed. In total, there were valid results from 100 samples and controls. Results showed that the modified VIP Gold for Listeria spp. is equivalent to the reference culture methods for the detection of Listeria.
Feldsine
Philip T
PT
BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA. ptf@biocontrolsys.com
Kerr
David E
DE
Shen
George
G
Lienau
Andrew H
AH
D000818:Animals; D003470:Culture Media; D003611:Dairy Products; D005504:Food Analysis; D005506:Food Contamination; D005516:Food Microbiology; D047468:Immunoprecipitation / Q000379:methods*; D008087:Listeria / Q000302:isolation & purification*; D012015:Reference Standards; D015203:Reproducibility of Results; D013193:Stainless Steel
D003470:Culture Media; D013193:Stainless Steel