Measuring autophagy in macrophages.
Macroautophagy is a conserved intracellular homeostatic mechanism for the degradation of cytosolic constituents. Autophagy can promote cell survival by providing essential amino acids from the breakdown of macromolecules during periods of nutrient deprivation, and can remove damaged or excess organelles, such as mitochondria and peroxisomes. More recently, autophagy has been shown to play an important role in innate and adaptive immune responses to pathogenic bacteria in macrophages and dendritic cells. This unit presents protocols for the measurement of autophagy in macrophages.
Harris
James
J
School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
Hanrahan
Orla
O
School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
De Haro
Sergio A
SA
University of New Mexico School of Medicine, Albuquerque, New Mexico.
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D002103:Cadaverine / Q000031:analogs & derivatives / Q000032:analysis; D003584:Cytological Techniques / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents / Q000737:chemistry; D008264:Macrophages / Q000166:cytology* / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000032:analysis; D010588:Phagosomes / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
D048868:Adaptor Proteins, Signal Transducing; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008869:Microtubule-Associated Proteins; C008542:monodansylcadaverine; D002103:Cadaverine