Selective protein patterning based on the micro-structured organosilane self-assembled monolayer by vacuum ultraviolet light lithography.
We have succeeded to immobilize fluorescent proteins selectively using a micro-structured organosilane self-assembled monolayer as a template. An organosilane layer with amino terminal group was formed on a thermally oxidized Si wafer by liquid-phase method and then was pattern-etched by vacuum ultraviolet light (VUV). The second organosilane layer with thiol terminal group was deposited on the etched area by chemical vapor surface modification method (CVSM). These micro-structured organosilane layer containing two reactive terminal groups were chemically modified using bi-functional linkers. Two kinds of fluorescent protein, Enhanced Cyan Fluorescent Protein (ECFP) and R-phycoerythrin were selectively immobilized on the chemically modified surface.
Lim
Sung-Hyuk
SH
Advanced Industrial Science and Technology, (AIST), Sapporo 062-8517, Japan.
Yamaguchi
Munehiro
M
Nishimura
Okio
O
Mie
Yasuhiro
Y
Tamura
Tomohiro
T
Kim
Byung-Woo
BW
Suzuki
Masaaki
M
D003460:Crystallization / Q000379:methods; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008904:Miniaturization; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D017646:Organosilicon Compounds / Q000737:chemistry* / Q000528:radiation effects; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D012821:Silanes / Q000737:chemistry* / Q000528:radiation effects; D013499:Surface Properties; D014466:Ultraviolet Rays; D014618:Vacuum
D046911:Macromolecular Substances; D017646:Organosilicon Compounds; D011506:Proteins; D012821:Silanes