Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake.
Four new fluorescent derivatives of cholic acid have been synthesized; they incorporate a dansyl moiety at 3alpha-, 3beta-, 7alpha- or 7beta- positions. These cholic acid analogs are UV photoactive and also exhibit green fluorescence. In addition, they have been demonstrated to be suitable for studying the kinetics of bile acid transport by flow cytometry.
Rohacova
Jana
J
Instituto de Tecnología Química-Departamento de Química (UPV-CSIC), Avda de los Naranjos s/n, E-46022, Valencia, Spain.
Marin
M Luisa
ML
Martínez-Romero
Alicia
A
O'Connor
José-Enrique
JE
Gomez-Lechon
M Jose
MJ
Donato
M Teresa
MT
Castell
Jose V
JV
Miranda
Miguel A
MA
D000818:Animals; D002793:Cholic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D003619:Dansyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D005453:Fluorescence; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008968:Molecular Conformation; D010777:Photochemistry; D051381:Rats; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D002793:Cholic Acids; D003619:Dansyl Compounds