[Expression of genes connected with nuclear factor kappa B (NFkappaB) estimated by oligonucleotide microarray analysis HG-U133A in patients with acute myocardial infarction].
Atherosclerosis is a permanently progressive chronic inflammatory disorder which nuclear factor kappaKB (NFkappaB) is involved. Therefore NFkappaB has become integral aspect of atherogenesis and its complications.


OBJECTIVE
Estimation of genes expression involved in NFkappaB signaling pathway and separation genes differentiate patients with acute myocardial infarction from healthy subjects.


METHODS
The examination was assess using the Affymetrix HG-U133A oligonucleotide microarray. Differentiating genes were determined using Bland-Altman graph analysis. Patients wasn't treated due to cardiac diseases before. All patients were subjected to 12-lead ECG, 2-D echocardiography, coronarography and laboratory studies including cardiac troponin, CK and CK-MB. The healthy individuals were subjected to coronarography and computed tomography (calcium score)--coronary artery disease was out of the question.


RESULTS
Hierarchical clusterization has demonstrated that the genes expression of patients with acute myocardial infarction was different from healthy individuals. It also demonstrated that the individual groups are homogeneous, especially the group of patients with acute myocardial infarction, regardless of diagnosis, number of risk factors and progression of coronary artery disease. Further Bland-Altman graph analysis showed three important differentiating genes: TLR2, TNFRSF1A i IKBKAP.


CONCLUSIONS
Our results confirmed the share of genes involved in NFkappaB signaling pathway in acute complications of atherosclerosis. Noticed differences in genes expression of patients with acute myocardial infarction and healthy subjects can show important role isolated differentiating genes in destabilization of atherogenic plaque and acute myocardial infarction occurrence.
Dabek
Jozefa
J
Slaski Uniwersytet Medyczny w Katowicach, Katedra i Klinika Kardiologii. jdabek@sum.edu.pl
Swiderski
Robert
R
Głogowska-Ligus
Joanna
J
Kułach
Andrzej
A
Gasior
Zbigniew
Z
D000328:Adult; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics; D018450:Disease Progression; D015870:Gene Expression; D006801:Humans; D009203:Myocardial Infarction / Q000235:genetics*; D015345:Oligonucleotide Probes; D040081:Protein Array Analysis; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000235:genetics; D035602:Transcriptional Elongation Factors
C497252:CEBPD protein, human; D002352:Carrier Proteins; C114720:Elp1 protein, human; D015345:Oligonucleotide Probes; D047888:Receptors, Tumor Necrosis Factor, Type I; C495342:TLR2 protein, human; C506235:TNFRSF1A protein, human; D051195:Toll-Like Receptor 2; D035602:Transcriptional Elongation Factors; D051743:CCAAT-Enhancer-Binding Protein-delta