title: A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
abstract: Various dendritic cell (DC) populations exist that differ in phenotype and ability to present antigen to T cells. For example, plasmacytoid DCs (pDCs) are less potent T cell activators compared with conventional DCs (cDCs). Here, we compared porcine blood DCs (BDCs), containing pDCs and cDCs, and monocyte-derived DCs (MoDC), consisting of cDCs, in their phenotype, ability to uptake antigen, activation and maturation and their ability to present antigen to autologous T cells. Pigs represent an important animal model, whose immune system in many respects closely resembles that of humans. For example, the distribution of Toll-like receptors is similar to that of humans, in contrast to that of mice. Here we demonstrate that both populations endocytose foreign material. Following lipopolysaccharide stimulation, CD80/86 and chemokine receptor (CCR)7 expression was increased in both populations as was the expression of the chemokine ligands (CCL)-2, CCL-4, CCL-20 and CXCL-2. Although basal and post-stimulation protein concentrations of interleukins 6 and 8 and tumour necrosis factor-alpha were higher in MoDCs, protein concentrations showed a higher fold increase in BDCs. Antigen-specific proliferation of autologous T cells was induced by MoDCs and BDCs. Interestingly, while MoDCs induced stronger proliferation in naive T cells, no difference in proliferation was observed when primed T cells were studied. These results demonstrate that isolated porcine BDCs are highly responsive to stimulation with lipopolysaccharide and are functionally able to drive primed T-cell proliferation to the same extent as MoDCs.
authors.affiliation: Vaccine & Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK.
authors.forename: Marina R
authors.initials: MR
authors.lastname: Facci
authors.forename: Gael
authors.initials: G
authors.lastname: Auray
authors.forename: Rachelle
authors.initials: R
authors.lastname: Buchanan
authors.forename: Jill
authors.initials: J
authors.lastname: van Kessel
authors.forename: David R
authors.initials: DR
authors.lastname: Thompson
authors.forename: Sarah
authors.initials: S
authors.lastname: Mackenzie-Dyck
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: Volker
authors.initials: V
authors.lastname: Gerdts
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D018925:Chemokines / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000276:immunology; D004705:Endocytosis / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D015870:Gene Expression / Q000187:drug effects; D016130:Immunophenotyping; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology*; D010047:Ovalbumin / Q000276:immunology; D054400:Receptors, CCR7 / Q000235:genetics; D034421:Sus scrofa; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015703:Antigens, CD; C515370:CCR7 protein, human; D018925:Chemokines; D016207:Cytokines; D003911:Dextrans; D008070:Lipopolysaccharides; D054400:Receptors, CCR7; C015219:fluorescein isothiocyanate dextran; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate