[Hepatitis B virus X protein regulates c-met promoter via the ERKs singal pathway in HepG2 cells].
OBJECTIVE
To explore the signal pathway mediating the regulatory effect of Hepatitis B virus X protein (HBX) on c-met gene promoter in HepG2 cells.


METHODS
The expression of c-met in HBX-transfected HepG2 cells treated with different signal pathway inhibitors was detected by western blot, the invasion capability of cells was determined by Matrigel invasion assay.


RESULTS
ERK inhibitor U0126 inhibited the expression of the c-Met in HBx-transfected HepG2 cells. However, both p38MAPK inhibitor SB203580 and PI-3K inhibitor wortmanin had no effect on expression of the c-Met in HBx-transfected HepG2 cells. Furthermore, the ERK inhibitor U0126 also inhibited the invasiveness of HBX-transfected HepG2 cells.


CONCLUSIONS
HBx induces invasion of HCC via activation of ERK pathway.
Xie
Bin
B
Department of Hepatobiliary Surgery, Daping Hospital and the Field Surgery Research Institute, Third Military Medical University, Chongqing 400042, China. xiebin313@yahoo.com.cn
Tang
Chun
C
Chen
Ping
P
Gou
Yuan-bin
YB
Yuan
Tao
T
Jin
Shi-long
SL
Zhou
Yu-yang
YY
D015153:Blotting, Western; D002070:Butadienes / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009570:Nitriles / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
D002070:Butadienes; D009570:Nitriles; D015534:Trans-Activators; C113580:U 0126; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D019859:Proto-Oncogene Proteins c-met; D048049:Extracellular Signal-Regulated MAP Kinases