A novel salt-tolerant mutant YWL-01 for the treatment of saline wastewater.
The treatment of high-saline wastewater from some salt-end markets including agro-food industry is a serious problem yet to be solved in some coastal cities. The conventional physical-chemical techniques are energy-consuming and their startup and running costs are still high. Biological methods using salt-tolerant bacterial strains for the treatment of hypersaline wastewater provide one possible solution. In this study, one salt-tolerant mutant named YWL-01 was screened out by sewage treatment and proved to be a genetically stable salt-tolerant strain for saline wastewater treatment. First, combined mutagenesis was done on an isolated sewage treatment strain Bacillus Y for the screening of salt tolerance, and 11 mutants were obtained after subculture for many times. Then, a secondary screening test was performed for COD (chemical oxygen demand) and TOC (total organic carbon) removal efficiency analyses. At last, the best mutant YWL-01 with increased capacity to treat saline wastewater was chosen for use. RAPD (Random Amplified Polymorphic DNA) analysis of genetic stability on the mutant YWL-01 showed that it is a hereditary mutant for the treatment of high-saline wastewater.
Liu
J
J
College of Life Science, Liaoning Normal University, Huanghe Road 850#, Dalian 116029, China. juaner-1983@hotmail.com
Wang
G L
GL
D001407:Bacillus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D001673:Biodegradation, Environmental; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D004361:Drug Tolerance; D009152:Mutagenicity Tests; D054712:Salinity; D012722:Sewage; D012965:Sodium Chloride / Q000494:pharmacology*; D014865:Waste Disposal, Fluid / Q000379:methods*
D003037:Cobalt Radioisotopes; D012722:Sewage; D012965:Sodium Chloride