title: The quality of stored umbilical cord and adult-donated whole blood in Mombasa, Kenya.
abstract: BACKGROUND
  In sub-Saharan Africa umbilical cord blood may be a useful source of blood for transfusion. Before clinical trials, evidence is needed that cord blood donations, which vary greatly in volume, can be collected and stored into a fixed volume of anticoagulant-preservative solution obviating the need for prestorage processing.
  
  
  METHODS
  Twenty-four umbilical cord whole blood (UC-WB) donations were collected into 21 mL of CPDA-1 and refrigerated for 35 days. The Kenya Blood Transfusion Service provided 12 adult-donated whole blood (AD-WB) controls. Supernatant hemoglobin (Hb) and potassium were assayed at 7-day intervals.
  
  
  RESULTS
  UC-WB red blood cell hemolysis and potassium loss increased throughout storage but did not differ significantly with cord blood volume. Hemolysis rates did not differ significantly between UC-WB and AD-WB but UC-WB potassium loss was slightly but significantly greater than AD-WB on Days 2, 7, and 14 (p < 0.05). In the AD-WB controls, eight were low volume (<405 mL), two had total Hb of less than 45 g, and two showed hemolysis greater than 0.8% by Day 28.
  
  
  CONCLUSIONS
  Variable volumes of UC-WB can be stored for 35 days without prestorage processing and further work into its suitability for transfusion to children is justified. The quality of conventional AD-WB is a concern and needs further evaluation.
authors.affiliation: Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute/Wellcome Trust Research Programme, Kilifi, Kenya. ohassall@gmail.com
authors.forename: Oliver
authors.initials: O
authors.lastname: Hassall
authors.forename: Kathryn
authors.initials: K
authors.lastname: Maitland
authors.forename: Gregory
authors.initials: G
authors.lastname: Fegan
authors.forename: Johnstone
authors.initials: J
authors.lastname: Thitiri
authors.forename: Lewa
authors.initials: L
authors.lastname: Pole
authors.forename: Robert
authors.initials: R
authors.lastname: Mwakesi
authors.forename: Douglas
authors.initials: D
authors.lastname: Denje
authors.forename: Kongo
authors.initials: K
authors.lastname: Wambua
authors.forename: Kishor
authors.initials: K
authors.lastname: Mandaliya
authors.forename: Imelda
authors.initials: I
authors.lastname: Bates
mesh_terms: D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D005260:Female; D005312:Fetal Blood; D006461:Hemolysis; D006801:Humans; D007630:Kenya; D008297:Male; D011786:Quality Control; D013997:Time Factors