title: A new sol-gel silica nanovehicle preparation for photodynamic therapy in vitro.
abstract: We report a facile silica nanovehicle preparation procedure for hydrophobic drug delivery, which is carried out in water without adding any surfactant and additional catalyst. This strategy includes hydrophobic drug nanopaticle preparation by reprecipitation method and in situ hydrolyzation and polymerization to encapsulate this naoparticle using only N-(beta-amimoethyl)-gamma-aminopropyltriethoxysilane (AETPS). To demonstrate this technique hypocrellin A (HA), a hydrophobic photosensitizing anticancer drug, is embedded into silica nanovehicle using this simple method. The resulting HA encapsulated nanovehicles (HANV) are monodisperse and stable in aqueous solution. Comparative studies with free HA and entrapped HA have demonstrated that the encapsulation effect on the embedded photosensitizer nanoparticle significantly enhances the efficacy of singlet oxygen generation and, thereby, the in vitro photodynamic efficacy.
authors.affiliation: Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wei
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Feng
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Zhou
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D011232:Chemical Precipitation; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D005782:Gels; D006367:HeLa Cells; D006801:Humans; D006860:Hydrogen Bonding; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000528:radiation effects; D014677:Pharmaceutical Vehicles / Q000737:chemistry* / Q000633:toxicity; D019800:Phenol; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000528:radiation effects; D011437:Propylamines; D011809:Quinones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry* / Q000633:toxicity; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors
chemical_list: D004337:Drug Carriers; D005782:Gels; D014677:Pharmaceutical Vehicles; D017319:Photosensitizing Agents; D011437:Propylamines; D011809:Quinones; D017382:Reactive Oxygen Species; D012821:Silanes; D026082:Singlet Oxygen; D019800:Phenol; D010569:Perylene; C477625:amino-propyl-triethoxysilane; C048388:hypocrellin A