title: [Amelogenin expression in cultured human dental pulp and periodontium related cells].
abstract: OBJECTIVE
  To investigate gene expression of amelogenin (Am) in human gingival epithelial cells(HGEC) and also other oral ectomesenchyme cells (human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells).
  
  
  METHODS
  The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.
  
  
  RESULTS
  There was no amelogenin expression detected in any of the cells.
  
  
  CONCLUSIONS
  These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China (Grant No.30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China. sheatinlee@hotmail.com
authors.forename: Xi-ting
authors.initials: XT
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wen
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Shao-yun
authors.initials: SY
authors.lastname: Jiang
mesh_terms: D053523:Amelogenin; D002460:Cell Line; D002478:Cells, Cultured; D003782:Dental Pulp; D004847:Epithelial Cells; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D010513:Periodontal Ligament; D010519:Periodontium
chemical_list: D053523:Amelogenin