Induction of T cells suppression by dendritic cells transfected with VSIG4 recombinant adenovirus.
VSIG4 has been recently described as a B7 family-related protein. The immunotherapeutic potential of dendritic cells (DCs) transfected with VSIG4 recombinant adenovirus has not been characterized. In the present study, DCs were transfected with human VSIG4 (hVSIG4) recombinant adenovirus, a novel costimulatory molecule known to be a potent inhibitor of T cell activation. Transfected DCs were cocultured with allogeneic T cells and proliferation, cytokine production and T cell activation marker expression were assessed. The results showed that T cell proliferation potential, cytokine production and activation marker expression were suppressed after coculture with hVSIG4 recombinant adenovirus-transfected DCs. These findings suggest that DCs transfected with hVSIG4 recombinant adenovirus are capable of inducing allogeneic T cell suppression, which represents an ideal strategy for manipulating the immune response to transplanation or autoimmune diseases.
Xu
Shuxiong
S
Department of Kidney, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Sun
Zhaolin
Z
Li
Lian
L
Liu
Jun
J
He
Jian
J
Song
Dalong
D
Shan
Gang
G
Liu
Hong
H
Wu
Xiongfei
X
D000256:Adenoviridae / Q000235:genetics; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D011951:Receptors, Complement / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D019076:Transgenes / Q000235:genetics
D015703:Antigens, CD; D000943:Antigens, Differentiation; D011951:Receptors, Complement; C414427:VSIG4 protein, human