Evaluation of desensitizing agents on dentin permeability and dentinal tubule occlusion: an in vitro study.
One hundred twelve specimens from bovine incisors were divided into eight groups: Group 1 (treated with 10% strontium chloride gel), Group 2 (treated with 2% sodium fluoride gel), Group 3 (treated with 2% stannous fluoride gel), Group 4 (treated with 5% potassium nitrate gel), Group 5 (treated with 10% potassium nitrate gel), Group 6 (treated with 3% potassium oxalate gel), Group 7 (treated with hydroxyethylcellulose gel), and Group 8 (which received no treatment). Dentinal tubules were exposed after 0.5 mm of deep abrasion using a carbide bur and EDTA gel application. After each treatment, dentin permeability, tubule occlusion, and chemical elements on dentin were analyzed. There was a significant difference among groups in dentin permeability (p < 0.05 ANOVA). Groups 4, 5, and 6 showed the lowest values, while Groups 1, 7, and 8 exhibited the highest. Groups 1, 2, 3, 7, and 8 showed open dentinal tubules, Groups 4 and 5 had partial tubule occlusion, and most of the tubules in Group 6 were obliterated. Energy-dispersive x-rays revealed similar chemical characteristics among the experimental agents used, with traces of strontium, fluoride, sodium, and potassium. Within the limits of the study, 3% potassium oxalate gel showed the best results in terms of dentin permeability and dentinal tubule occlusion.
Oberg
Carolina
C
Department of Dentistry, State University of Ponta Grossa, Parana, Brazil.
Pochapski
Marcia Thais
MT
Farago
Paulo Vitor
PV
Granado
Carlos Jose Fernandes
CJ
Pilatti
Gibson Luiz
GL
Santos
Fabio Andre
FA
D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D003806:Dentin Permeability / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D004577:Electron Probe Microanalysis; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009566:Nitrates / Q000494:pharmacology; D010070:Oxalates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D016622:Silver Staining; D016369:Smear Layer; D012969:Sodium Fluoride / Q000494:pharmacology; D013324:Strontium / Q000494:pharmacology; D014002:Tin Fluorides / Q000494:pharmacology; D014072:Tooth Abrasion / Q000473:pathology
D002614:Chelating Agents; D057133:Dentin Desensitizing Agents; D009566:Nitrates; D010070:Oxalates; D017680:Potassium Compounds; D014002:Tin Fluorides; D012969:Sodium Fluoride; D002482:Cellulose; C002283:hydroxyethylcellulose; D004492:Edetic Acid; C025700:strontium chloride; C023844:potassium nitrate; D013324:Strontium