title: Nanoparticles incorporated in pH-sensitive hydrogels as amoxicillin delivery for eradication of Helicobacter pylori.
abstract: A variety of approaches have been studied to overcome the problems encountered with using antibiotics, which are ineffective in treating Helicobacter pylori infections. In our study, chitosan/poly-gamma-glutamic acid nanoparticles incorporated into pH-sensitive hydrogels were developed as an efficient carrier for amoxicillin delivery. Our results indicate that hydrogels are pH-sensitive, leading to protecting nanoparticles from being destructed by gastric acid. The results of drug releasing in vitro study clearly indicate that the amount of amoxicillin released from nanoparticles incorporated in hydrogels at pH 1.2 was relatively low (14%), compared to that from only nanoparticles (50%). Confocal laser scanning microscopy revealed that nanoparticles could infiltrate cell-cell junctions and interact with H. pylori infection sites in the intercellular spaces. Additionally, the incorporation of amoxicillin-loaded nanoparticles in a hydrogel protected the drug from the actions of the gastric juice and facilitated amoxicillin interaction specifically with intercellular spaces, the site of H. pylori infection.
authors.affiliation: School of Chinese Medicine and Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China.
authors.forename: Chiung-Hung
authors.initials: CH
authors.lastname: Chang
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Yeh
authors.forename: Yi-Chi
authors.initials: YC
authors.lastname: Chen
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Chiou
authors.forename: Yuan-Man
authors.initials: YM
authors.lastname: Hsu
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000382:microbiology; D000658:Amoxicillin / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005750:Gastric Juice / Q000187:drug effects / Q000378:metabolism; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects*; D020100:Hydrogels / Q000008:administration & dosage / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000382:microbiology; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D020100:Hydrogels; D000658:Amoxicillin; D048271:Chitosan