Adaptive immune responses during Shigella dysenteriae type 1 infection: an in vitro stimulation with 57 kDa major antigenic OMP in the presence of anti-CD3 antibody.
An effort was made to understand the role of the 57 kDa major antigenic fraction of Shigella outer membrane protein (OMP) in the presence of T-cell antigen receptor in activation of adaptive immune responses of the cell mediated immune (CMI) restored patients. The expression of HLA-DR/CD4 out of CD3(+) T-cells was significantly dominant over the HLA-DR/CD8 and comparable to unstimulated cells of infected or healthy controls. CD4(+) T-cell activation together with HLA-DR is associated with the expression of CD25(+) (IL2Ralpha) for IL-2 growth factors with decreased IL-4 levels, required for maintaining the homeostasis of CD4(+) T cell. Furthermore, the positive expression of the CD45 antigen is possibly required for acquiring the memory for CD4(+) cells signals and facilitates the interaction with CD54 antigen. As a result, antigen-specific secondary signal is generated for B-cell activation to produce IgG2a and IgG2b. This suggests that antibody mediated-adaptive immune responses are generated due to anti-CD3 induced helper T-cell activity. The above mentioned findings reflect that the antigen alone might not exacerbate the selective T-cell responses. But these antigens in the presence of anti-CD3 antibody might help to elicit adaptive immune response via T-cell receptor (TCR) activation.
Bagchi
Ashim Kumar
AK
Microbial-Immunology Division, Research and Development, Nutratech Inc, Winnipeg, MB, Canada. ashim_k_bagchi333@yahoo.co.in
Sinha
Ajoy Kumar
AK
Adhikari
Rushita
R
Mukherjee
Joydeep
J
D056704:Adaptive Immunity; D000293:Adolescent; D000906:Antibodies / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002648:Child; D016207:Cytokines / Q000276:immunology; D004405:Dysentery, Bacillary / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012762:Shigella dysenteriae / Q000276:immunology* / Q000472:pathogenicity; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000906:Antibodies; D001425:Bacterial Outer Membrane Proteins; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D053645:Interleukin-2 Receptor alpha Subunit; D011948:Receptors, Antigen, T-Cell; D018799:Intercellular Adhesion Molecule-1; D017493:Leukocyte Common Antigens