In vitro effects of food extracts on selected probiotic and pathogenic bacteria.
A panel of 148 extracts from 37 food products was prepared using organic and aqueous solvents and both neutral and acidic conditions. The panel of food products tested included fruits, vegetables, grains, herbs and spices, most of which are common in a normal European-style diet. The impact of these extracts on the growth of selected probiotic bacteria (Lactobacillus reuteri, Lactobacillus rhamnosus, Bifidobacteria lactis) and pathogenic bacteria (Escherichia coli 0157:H7 and Escherichia coli LF82) was assessed using a standard minimum inhibitory concentration method. The results showed that aqueous extractions of garlic and black peppercorns significantly enhanced the growth of one strain of probiotic bacteria (L. reuteri) whilst inhibiting both pathogenic strains of E. coli at a 1:50 dilution. Aqueous extracts of banana, apple and orange all enhanced the growth of the three probiotic strains significantly, and inhibited the pathogens to approximately 80% of the controls (not significant). Both aqueous and organic extractions of ginger significantly inhibited the growth of one or both E. coli strains, respectively (also at the 1:50 dilution).
Sutherland
Juliet
J
New Zealand Institute for Crop & Food Research Ltd, Palmerston North, New Zealand. SutherlandJ@crop.cri.nz
Miles
Michelle
M
Hedderley
Duncan
D
Li
Jessie
J
Devoy
Sarah
S
Sutton
Kevin
K
Lauren
Denis
D
D000363:Agaricales / Q000737:chemistry; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D005502:Food; D041981:Gastrointestinal Tract / Q000382:microbiology; D006304:Health Status; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development*; D008826:Microbial Sensitivity Tests; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D019936:Probiotics; D012997:Solvents; D013662:Tea / Q000737:chemistry
D010936:Plant Extracts; D012997:Solvents; D013662:Tea