[Preparation and property of platinum microcoil modified by a copolymer-VEGF conjugate].
OBJECTIVE
To prepare a platinum microcoil coated with polymers and vascular endothelial growth factor (VEGF), and evaluate its surface characteristics and property of sustained VEGF release.


METHODS
The surface of the platinum microcoils (GDC) were modified by coating P(DLLA-co-TMC) copolymer and immobilizing heparin on the surface of GDC. VEGF was then loaded onto the surface of GDC and the controlled release of VEGF within GDC was achieved. The morphology was observed by scanning electron microscope, and the sustained release of VEGF was evaluated by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Platinum coils were prepared by successive deposition of P(DLLA-co-TMC) copolymer and anionic heparin, and VEGF was immobilized through affinity interaction with heparin. The accumulative release of VEGF increased obviously during the entire testing period without burst release.


CONCLUSIONS
The use of P(DLLA-co-TMC) copolymer allows immobilization of VEGF on the platinum coils for controlled VEGF release, and improves the biological property of the coils.
Wang
Qiu-Jing
QJ
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. qiujingwang@163.com
Sun
Xin-Lin
XL
Ji
Bin
B
Zhu
Ai-Ping
AP
Quan
Da-Ping
DP
D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000494:pharmacology*; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; D010984:Platinum