Effect of treatment with cadmium on structure-function relationships in rat liver mitochondria: studies on oxidative energy metabolism and lipid/phospholipids profiles.
Effects of treatment with a single intraperitoneal injection of cadmium (Cd) on oxidative energy metabolism and lipid/phospholipid profiles of rat liver mitochondria were examined at the end of 1 week and 1 month. Following Cd treatment the body weight increased only in the 1 month group, whereas the liver weight increased in both groups. State 3 and 4 respiration rates in general decreased significantly, with the maximum effect being seen with succinate. The 1 week Cd group showed decreased respiratory activity with glutamate, pyruvate + malate, and succinate as the substrates. In the 1 month Cd-treated group respiration rates recovered with glutamate and pyruvate + malate but not with succinate. All cytochrome contents decreased in the 1 week Cd-treated group but recovered in the 1 month group. ATPase activity registered an increase in both Cd-treated groups. Dehydrogenase activities increased in the 1 week group but decreased in the 1 month Cd-treated group. The mitochondrial cholesterol content increased in the 1 week Cd-treated group. In the 1 week Cd-treated group the lysophospholipid (Lyso), sphingomyelin (SPM), and diphosphatidylglycerol (DPG) components increased. By contrast, the phosphatidylethanolamine (PE) component decreased. In the 1 month Cd-treated group the phosphatidylinositol, phosphatidylserine, and DPG components increased, whereas the Lyso, SPM, and phosphatidylcholine components decreased. The results demonstrate that single-dose Cd treatment can have adverse effects on liver mitochondrial oxidative energy metabolism and lipid/phosphopholipid profiles, which in turn can affect membrane structure-function relationships.
Modi
Hiren R
HR
Department of Biochemistry, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390 002, India. modi_hiren@yahoo.co.in
Katyare
Surendra S
SS
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
D010743:Phospholipids; D002104:Cadmium; D013384:Succinate Cytochrome c Oxidoreductase; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D000251:Adenosine Triphosphatases