Factors influencing survival of enterotoxigenic Escherichia coli, Salmonella enterica (serovar Typhimurium) and Vibrio parahaemolyticus in marine environments.
The presence and persistence of enterotoxigenic Escherichia coli (ETEC) is poorly investigated in marine habitats. Here we compared ETEC with the more studied fecal contaminant, Salmonella enterica serotype Typhimurium (S. enterica) and the marine bacteria Vibrio parahaemolyticus. All three species of bacteria were culturable on agar plates during 8 weeks of incubation in seawater. However, the culturability of ETEC was positively affected by low temperature whereas V. parahaemolyticus was negatively affected. High-nutrient conditions favored the growth of ETEC but not the other bacteria. When the bacteria were fed to blue mussels, V. parahaemolyticus inhibited the filtration activity and the ingestion was lower than that of the enterobacteria. On the other hand, the mussels were less efficient in eliminating V. parahaemolyticus and an in vitro study showed that the hemocytes of three different species of bivalves were not able to kill this strain of V. parahaemolyticus. The bactericidal capacity of bivalves was seemingly an efficient elimination pathway for S. enterica and ETEC. This study showed that ETEC in endemic areas should, to the same degree as S. enterica and V. parahaemolyticus, be taken in consideration when assessing the role of marine environments as a source of enteric infection.
Hernroth
Bodil
B
The Royal Swedish Academy of Sciences, Sven Lovén Centre of Marine Sciences, Kristineberg, Fiskebäckskil, Sweden. bodil.hernroth@marecol.gu.se
Lothigius
Asa
A
Bölin
Ingrid
I
D000818:Animals; D049872:Bivalvia / Q000276:immunology / Q000382:microbiology; D054307:Enterotoxigenic Escherichia coli / Q000254:growth & development*; D006434:Hemocytes / Q000276:immunology; D019779:Salmonella enterica / Q000254:growth & development*; D012623:Seawater / Q000382:microbiology*; D013696:Temperature; D014466:Ultraviolet Rays; D014736:Vibrio parahaemolyticus / Q000254:growth & development*; D014871:Water Microbiology