[siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis].
OBJECTIVE
To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation.


METHODS
Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later.


RESULTS
In the oocytes without any siRNA injection, oocyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In oocytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01).


CONCLUSIONS
siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.
Zhang
Ren-Li
RL
Center of Reproductive Medicine, Guanddong Academy of Medical Sciences (Guangdong Provincial People's Hospital), Guangzhou 510080, China. renlizhangm@yahoo.com.cn
Meng
Jin-Xiu
JX
Wen
An-Ming
AM
Huang
Yue-Shen
YS
Zhou
Can-Quan
CQ
Wang
Kai
K
Jia
Lei
L
Liu
Cai-Xia
CX
Deng
Xin-Yan
XY
Chen
Xi-Gu
XG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D020552:Comet Assay; D005260:Female; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics* / Q000502:physiology; D014162:Transfection
D034741:RNA, Small Interfering; D013108:Sphingomyelin Phosphodiesterase