Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant.
Our interest in development of hyaluronidase inhibitors as male antifertility agents led to identification of Terminalia chebula (T. chebula) plant with hyaluronidase (HAase) inhibitory activity of human spermatozoa ( approximately 93% inhibition) and rat caudal epididymal spermatozoa ( approximately 86% inhibition) in vitro at 30 mg/ml. We further demonstrated inhibition of hyaluronidase activity of testis and epididymal spermatozoa in vivo coincident with antispermatogenic activity and contraceptive efficacy of TC extract administered at 50 and 100mg/kg/day orally for 60 days in male albino rats. The significant decrease in motility, count and increase in morphological abnormalities of epididymal spermatozoa and severe reduction in fertility (-100%) of male rats treated with T. chebula fruit extract at 100mg/kg dose could be attributed to either direct effect on testis or direct or indirect interference with sperm maturation in epididymis, and/or inhibition of testicular and epididymal sperm hyaluronidase enzyme in vivo probably caused by flavonoids like tannins present in T. chebula.
Srivastav
Archana
A
Division of Endocrinology, Central Drug Research Institute, Lucknow, India. archana1849@gmail.com
Chandra
Abhishek
A
Singh
Madhulika
M
Jamal
Farrukh
F
Rastogi
Preeti
P
Rajendran
Siron Mani
SM
Bansode
Falgun Wanganuji
FW
Lakshmi
Vijai
V
D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects; D005298:Fertility / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D029766:Terminalia / Q000737:chemistry*; D013737:Testis / Q000187:drug effects / Q000473:pathology
D000988:Antispermatogenic Agents; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D006821:Hyaluronoglucosaminidase