title: Molecular cloning and functional characterization of a mouse gene upregulated by lipopolysaccharide treatment reveals alternative splicing.
abstract: Treatment of mouse cells with lipopolysaccharide (LPS) potently initiates an inflammatory response, but the underlying mechanisms are unclear. We therefore sought to characterize cDNA sequences of a new mouse LPS-responsive gene, and to evaluate the effects of MLrg. Full-length cDNAs were obtained from LPS-treated NIH3T3 cells. We report that the MLrg gene produces two alternative splice products (GenBank Accession Nos. DQ316984 and DQ320011), respectively, encoding MLrgW and MLrgS polypeptides. Both proteins contain zinc finger and leucine zipper domains and are thus potential regulators of transcription. Expression of MLrgW and MLrgS were robustly upregulated following LPS treatment, and the proteins were localized predominantly in the nuclear membrane and cytoplasm. In stable transfectants over-expressing MLrgW the proportion of cells in G1 phase was significantly reduced, while in cells over-expressing MLrgS the proportion of cells in G2 was significantly increased; both proteins are thus potential regulators of cell cycle progression. Upregulation of MLrgW and MLrgS may be an important component of the LPS inflammatory pathway and of the host response to infection with GNB.
authors.affiliation: Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Du
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Chen
authors.forename: Zongming
authors.initials: Z
authors.lastname: Dai
authors.forename: Yuan
authors.initials: Y
authors.lastname: Bi
authors.forename: Tongjian
authors.initials: T
authors.lastname: Cai
authors.forename: Lichao
authors.initials: L
authors.lastname: Hou
authors.forename: Yubo
authors.initials: Y
authors.lastname: Chai
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Song
authors.forename: Sumin
authors.initials: S
authors.lastname: Chen
authors.forename: Wenjing
authors.initials: W
authors.lastname: Luo
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Chen
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D056970:Co-Repressor Proteins; D006090:Gram-Negative Bacteria / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000382:microbiology*; D016350:Leucine Zippers; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D008070:Lipopolysaccharides; D009687:Nuclear Proteins; C502605:Txlng protein, mouse