title: Verification of complete bisulfite modification using Calponin-specific primer sets.
abstract: OBJECTIVE
  To verify complete bisulfite modification.
  
  
  METHODS
  Bisulfite modified DNA was screened by PCR with Calponin-specific primer sets prior to detecting methylation status of 14-3-3sigma by methylation-specific PCR.
  
  
  RESULTS
  False positive methylation was obtained from incomplete bisulfite modification. The lower detection limit of wild type Calponin-specific primer is approximately 0.375 ng of unmodified DNA.
  
  
  CONCLUSIONS
  Verification of complete bisulfite modification by Calponin-specific primer sets can reduce misinterpretation of methylation.
authors.affiliation: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Ruethairat
authors.initials: R
authors.lastname: Sriraksa
authors.forename: Patimaporn
authors.initials: P
authors.lastname: Chaopatchayakul
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.forename: Chanvit
authors.initials: C
authors.lastname: Leelayuwat
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers / Q000378:metabolism*; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013447:Sulfites / Q000378:metabolism*; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D017931:DNA Primers; D008840:Microfilament Proteins; D013447:Sulfites; D000096985:Calponins; C042345:hydrogen sulfite