title: Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release.
abstract: BACKGROUND
  Rheumatoid arthritis is an inflammatory disease marked by intra-articular decreases in pH, aberrant hyaluronan regulation and destruction of bone and cartilage. Acid-sensing ion channels (ASICs) are the primary acid sensors in the nervous system, particularly in sensory neurons and are important in nociception. ASIC3 was recently discovered in synoviocytes, non-neuronal joint cells critical to the inflammatory process.
  
  
  OBJECTIVE
  To investigate the role of ASIC3 in joint tissue, specifically the relationship between ASIC3 and hyaluronan and the response to decreased pH.
  
  
  METHODS
  Histochemical methods were used to compare morphology, hyaluronan expression and ASIC3 expression in ASIC3+/+ and ASIC3-/- mouse knee joints. Isolated fibroblast-like synoviocytes (FLS) were used to examine hyaluronan release and intracellular calcium in response to decreases in pH.
  
  
  RESULTS
  In tissue sections from ASIC3+/+ mice, ASIC3 localised to articular cartilage, growth plate, meniscus and type B synoviocytes. In cultured FLS, ASIC3 mRNA and protein was also expressed. In FLS cultures, pH 5.5 increased hyaluronan release in ASIC3+/+ FLS, but not ASIC3-/- FLS. In FLS from ASIC3+/+ mice, approximately 50% of cells (25/53) increased intracellular calcium while only 24% (14/59) showed an increase in ASIC3-/- FLS. Of the cells that responded to pH 5.5, there was significantly less intracellular calcium increases in ASIC3-/- FLS compared to ASIC3+/+ FLS.
  
  
  CONCLUSIONS
  ASIC3 may serve as a pH sensor in synoviocytes and be important for modulation of expression of hyaluronan within joint tissue.
authors.affiliation: Graduate Program in Physical Therapy and Rehabilitation Science, 1-242 MEB, University of Iowa, Iowa City, Iowa 52242, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Kolker
authors.forename: R Y
authors.initials: RY
authors.lastname: Walder
authors.forename: Y
authors.initials: Y
authors.lastname: Usachev
authors.forename: J
authors.initials: J
authors.lastname: Hillman
authors.forename: D L
authors.initials: DL
authors.lastname: Boyle
authors.forename: G S
authors.initials: GS
authors.lastname: Firestein
authors.forename: K A
authors.initials: KA
authors.lastname: Sluka
mesh_terms: D062565:Acid Sensing Ion Channels; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015222:Sodium Channels / Q000378:metabolism / Q000502:physiology*; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*
chemical_list: C508378:ASIC3 protein, mouse; D062565:Acid Sensing Ion Channels; D015222:Sodium Channels; D006820:Hyaluronic Acid; D002118:Calcium