[The effect of VEGF-ASODN transfection on expression of VEGF and growth in tongue squamous cancer cell line Tca8113].
OBJECTIVE
To evaluate the effect of transfection of vascular endothelial growth factor antisense oligonucleotide (VEGF-ASODN) on expression of vascular endothelial growth factor(VEGF) and growth in tongue cancer cells.


METHODS
The VEGF-ASODN was synthesized with phosphorothioic acid. After transfecting with VEGF-ASODN in tongue cancer cells Tca8113, the quantity of VEGFmRNA and protein was detected by hybridization in situ and immunocytochemistry, and the quantity of VEGF protein in supernatant was detected by ELISA. FCM and MTT methods were used to detect cellular apoptosis and the activity of cells, respectively. The effect of transfection on the growth of cells was evaluated by growth curve. SPSS12.0 software package was used for statistical analysis.


RESULTS
The quantity of VEGF mRNA, protein levels of VEGF in the cells and in culture fluid decreased after transfection of VEGF-ASODN (P<0.01,P<0.05).The ratio of apoptosis increased, the activity of cells also decreased after transfection VEGF-ASODN (P<0.01).


CONCLUSIONS
Transfection with VEGF-ASODN in Tca8113 cells can inhibit the expression of VEGF remarkably. It can enhance cellular apoptosis and suppress growth of cells. Supported by Research Fund from Science and Technology Bureau of Taian City (Grant No.2007-46).
Li
Xiao-guang
XG
Department of Stomatology, Taian City Central Hospital, Shandong Province, China. lxg0538@163.com
Wang
Yan-xiu
YX
Zhou
Bao-qin
BQ
Qi
Chun-hua
CH
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D018307:Neoplasms, Squamous Cell; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D014062:Tongue Neoplasms; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A