High-sensitivity express immunochromatographic method for detection of plant infection by tobacco mosaic virus.
A highly sensitive express immunochromatography method for molecular diagnosis of plant virus infections was elaborated on the example of a model object - tobacco mosaic virus (TMV). The analysis time does not exceed 5 min, and the lower limit of TMV detection in non-clarified leaf extract (2-4 ng/ml) is comparable with the sensitivity of the enzyme-linked immunosorbent assay of the virus. A single measurement requires 0.1-0.2 ml tested solution (extract from 10-20 mg of leaf material). The sensitivity of TMV determination in the leaf tissue extract was increased by more than one order of magnitude using signal enhancement by silver and is 0.1 ng/ml. In this case, analysis time did not exceed 25 min. The simplicity of this method makes it especially convenient in express diagnosis of numerous analyzed specimens. The prototype of a diagnostic kit for serial analyses of plant viral infections both in laboratory and field conditions was elaborated.
Drygin
Yu F
YF
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. drygin@belozersky.msu.ru
Blintsov
A N
AN
Osipov
A P
AP
Grigorenko
V G
VG
Andreeva
I P
IP
Uskov
A I
AI
Varitsev
Yu A
YA
Anisimov
B V
BV
Novikov
V K
VK
Atabekov
J G
JG
D000914:Antibodies, Viral / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D053768:Metal Nanoparticles; D010935:Plant Diseases / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral