Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA.
A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines.
Yun
T
T
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
Ni
Z
Z
Liu
G Q
GQ
Yu
B
B
Chen
L
L
Huang
J G
JG
Zhang
Y M
YM
Chen
J P
JP
D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D004372:Ducks; D004371:Hepatitis Virus, Duck / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*; D016019:Survival Analysis; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral