title: Transplantation of mesenchymal stem cells exerts a greater long-term effect than bone marrow mononuclear cells in a chronic myocardial infarction model in rat.
abstract: The aim of this study is to assess the long-term effect of mesenchymal stem cells (MSC) transplantation in a rat model of chronic myocardial infarction (MI) in comparison with the effect of bone marrow mononuclear cells (BM-MNC) transplant. Five weeks after induction of MI, rats were allocated to receive intramyocardial injection of 10(6) GFP-expressing cells (BM-MNC or MSC) or medium as control. Heart function (echocardiography and (18)F-FDG-microPET) and histological studies were performed 3 months after transplantation and cell fate was analyzed along the experiment (1 and 2 weeks and 1 and 3 months). The main findings of this study were that both BM-derived populations, BM-MNC and MSC, induced a long-lasting (3 months) improvement in LVEF (BM-MNC: 26.61 +/- 2.01% to 46.61 +/- 3.7%, p < 0.05; MSC: 27.5 +/- 1.28% to 38.8 +/- 3.2%, p < 0.05) but remarkably, only MSC improved tissue metabolism quantified by (18)F-FDG uptake (71.15 +/- 1.27 to 76.31 +/- 1.11, p < 0.01), which was thereby associated with a smaller infarct size and scar collagen content and also with a higher revascularization degree. Altogether, results show that MSC provides a long-term superior benefit than whole BM-MNC transplantation in a rat model of chronic MI.
authors.affiliation: Hematology and Cell Therapy and Division of Cancer, Clinica Universitaria and Foundation for Applied Medical Research, University of Navarra, Navarra, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mazo
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Gavira
authors.forename: Gloria
authors.initials: G
authors.lastname: Abizanda
authors.forename: Cristina
authors.initials: C
authors.lastname: Moreno
authors.forename: Margarita
authors.initials: M
authors.lastname: Ecay
authors.forename: Mario
authors.initials: M
authors.lastname: Soriano
authors.forename: Pablo
authors.initials: P
authors.lastname: Aranda
authors.forename: María
authors.initials: M
authors.lastname: Collantes
authors.forename: Eduardo
authors.initials: E
authors.lastname: Alegría
authors.forename: Juana
authors.initials: J
authors.lastname: Merino
authors.forename: Iván
authors.initials: I
authors.lastname: Peñuelas
authors.forename: José Manuel
authors.initials: JM
authors.lastname: García Verdugo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelacho
authors.forename: Felipe
authors.initials: F
authors.lastname: Prósper
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods* / Q000473:pathology; D002478:Cells, Cultured; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology; D013995:Time; D013997:Time Factors; D016896:Treatment Outcome; D020257:Ventricular Remodeling / Q000502:physiology