title: A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites.
abstract: HPLC based activity profiling is an effective strategy to accelerate the discovery of new hits and leads from nature. It conveniently combines the superior separation power of HPLC micro scale compound separation with miniaturized biological screening methods, and on-line and off-line spectroscopy (PDA, MS(n), HR-MS, NMR) for structure elucidation. We here describe a protocol for the discovery of natural products with antimalarial, antileishmanial and antitrypanosomal activity, from extracts libraries in 96-well format. Analytical gradient HPLC on a 3 x 150 mm column of 350 microg of extract, and collection of one-minute fractions into 96 deep-well microtiter plates, parallel evaporation of the micro-fractions, and a suitable dilution scheme permitted parallel activity profiling against three parasites from a single HPLC injection. The protocol was validated with extracts and positive controls such as Artemisia annua.
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Adams
authors.forename: Stefanie
authors.initials: S
authors.lastname: Zimmermann
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Reto
authors.initials: R
authors.lastname: Brun
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000187:drug effects*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000913:Antibodies, Protozoan; D001688:Biological Products; D045424:Complex Mixtures; D010936:Plant Extracts