title: Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID.
abstract: The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the TFIID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.
authors.affiliation: Department of Structural Biology, Fairchild Building, Stanford University School of Medicine, Stanford, CA 94305, USA. hael@stanford.edu
authors.forename: Hans
authors.initials: H
authors.lastname: Elmlund
authors.forename: Vera
authors.initials: V
authors.lastname: Baraznenok
authors.forename: Tomas
authors.initials: T
authors.lastname: Linder
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szilagyi
authors.forename: Reza
authors.initials: R
authors.lastname: Rofougaran
authors.forename: Anders
authors.initials: A
authors.lastname: Hofer
authors.forename: Hans
authors.initials: H
authors.lastname: Hebert
authors.forename: Martin
authors.initials: M
authors.lastname: Lindahl
authors.forename: Claes M
authors.initials: CM
authors.lastname: Gustafsson
mesh_terms: D020285:Cryoelectron Microscopy; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D011487:Protein Conformation; D012568:Schizosaccharomyces / Q000737:chemistry*; D035181:TATA-Box Binding Protein / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D046912:Multiprotein Complexes; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D004247:DNA