title: Computerised analysis of standardised ultrasonographic images to monitor the repair of surgically created core lesions in equine superficial digital flexor tendons following treatment with intratendinous platelet rich plasma or placebo.
abstract: The effectiveness of new therapies to treat tendon injuries is difficult to determine and is often based on semi-quantitative methods, such as grey level analysis of ultrasonographic images or subjective pain scores. The alternatives are costly and long-lasting end-stage studies using experimental animals. In this study, a method of ultrasonographic tissue characterisation (UTC), using mathematical analysis of contiguous transverse ultrasonographic images, was used for intra-vital monitoring of the healing trajectory of standardised tendon lesions treated with platelet rich plasma (PRP) or placebo. Using UTC it was possible to detect significant differences between the groups in the various phases of repair. At end stage, over 80% of pixels showed correct alignment in the PRP group, compared with just over 60% in the placebo group (P<0.05). UTC also showed significant differences in the course of the healing process between PRP treated and placebo treated animals throughout the experiment. It was concluded that computerised analysis of ultrasonographic images is an excellent tool for objective longitudinal monitoring of the effects of treatments for superficial digital flexor tendon lesions in horses.
authors.affiliation: Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. g.bosch@uu.nl
authors.forename: Gerco
authors.initials: G
authors.lastname: Bosch
authors.forename: P
authors.initials: P
authors.lastname: René van Weeren
authors.forename: Ab
authors.initials: A
authors.lastname: Barneveld
authors.forename: Hans T M
authors.initials: HT
authors.lastname: van Schie
mesh_terms: D000818:Animals; D005260:Female; D006734:Horse Diseases / Q000000981:diagnostic imaging* / Q000628:therapy*; D006736:Horses / Q000293:injuries; D008297:Male; D053657:Platelet-Rich Plasma; D013708:Tendon Injuries / Q000000981:diagnostic imaging / Q000628:therapy / Q000662:veterinary*; D013710:Tendons / Q000000981:diagnostic imaging / Q000473:pathology; D014463:Ultrasonography; D014945:Wound Healing / Q000502:physiology*