[Evaluation of the factor which has an influence on red blood cell deformability in patients with end-stage renal disease].
To explore the calcium homoeostasis of red blood cells (RBC) in patients with end-stage renal disease (ESRD), which plays an important role in RBC deformability and survival, we measured free calcium-ion concentration of RBC([Ca2+]i). [Ca2+]i was measured by fluorescent spectrophotometry using fura-2. The subjects constituted 5 groups; normal controls(C group), patients with diabetes mellitus(DM) without renal failure (DM group), ESRD patients with DM (HD-DM group), non-diabetic ESRD patients with lower intact parathyroid hormone (i-PTH)(HD-NDM-A group), and with higher i-PTH(HD-NDM-B group). [Ca2] i was significantly higher in the HD-NDM group than in the C group, in the HD-DM group than in the DM group, in the DM group than in the C group, in the HD-DM group than in the HD-NDM group, and in the HD-NDM-B group than in the HD-NDM-A group. There was a significant positive correlation between i PTH and [Ca2+]i, and between C-PTH and [Ca2+]i. In stepwise multivariate regression analysis, ESRD, DM, and secondary hyperparathyroidism (SHPT) appeared as independent determinants of [Ca2+]i. [Ca2+]i was significantly decreased after a hemodialysis session comparing to that of before the session. These data indicate that [Ca2+] i of RBC is elevated in ESRD probably due to uraemia, DM, and SHPT.
Tateishi
Kinya
K
Department of Medical Technology, Faculty of Health Sciences, Kagawa Prefectural College of Health Sciences, Takamatsu 761-0123, Japan. tateishi@chs.pref.kagawa.jp
Fujita
Jun
J
Taminato
Tomohiko
T
D002118:Calcium / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood; D004907:Erythrocyte Deformability / Q000502:physiology*; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000097:blood / Q000150:complications; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications; D006435:Renal Dialysis
D002118:Calcium