Identification and partial characterization of a dynamin-like protein, EhDLP1, from the protist parasite Entamoeba histolytica.
The dynamin superfamily of proteins includes a large repertoire of evolutionarily conserved GTPases that interact with different subcellular organelle membranes in eukaryotes. Dynamins are thought to participate in a number of cellular processes involving membrane remodeling and scission. Dynamin-like proteins (DLPs) form a subfamily of this vast class and play important roles in cellular processes, such as mitochondrial fission, cytokinesis, and endocytosis. In the present study, a gene encoding a dynamin-like protein (EhDLP1) from the protist parasite Entamoeba histolytica was identified and the protein was partially characterized using a combination of in silico, biochemical, and imaging methods. The protein was capable of GTP binding and hydrolysis, lipid binding, and oligomerization. Immunofluorescence studies showed the protein to be associated with the nuclear membrane. A mutant of EhDLP1 lacking GTP binding and hydrolyzing activities did not associate with the nuclear membrane. The results suggest a nucleus-associated function for EhDLP1.
Jain
Ruchi
R
School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, India.
Shrimal
Shiteshu
S
Bhattacharya
Sudha
S
Bhattacharya
Alok
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D003001:Cloning, Molecular; D034281:Dynamins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D053058:Trophozoites / Q000378:metabolism / Q000648:ultrastructure
D007527:Isoenzymes; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D034281:Dynamins