title: [New derivatives of azobenzene for the directed modification of proteins].
abstract: Derivatives of azobenzene which contained a maleimide group in one of the benzene rings (for binding to a protein cysteine residue) and maleimide, hydroxyl, or carboxyl substitutes in another benzene ring were synthesized. The reactivity of these compounds towards a cysteine residue of a protein and their optical properties in a free state and after their attachment to the mutant forms of the SsoII restriction endonuclease were studied.
authors.affiliation: Chemical Faculty, Faculty of Bioengineering and Bioinformation Science, Belozerskii Institute of Physicochemical Biology, Moscow State University, Moscow, Russia.
authors.forename: Le Tkhi
authors.initials: le T
authors.lastname: Khien
authors.forename: B
authors.initials: B
authors.lastname: Shirling
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Riazanova
authors.forename: T S
authors.initials: TS
authors.lastname: Zatsepin
authors.forename: E M
authors.initials: EM
authors.lastname: Volkov
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: T I
authors.initials: TI
authors.lastname: Velichko
authors.forename: A
authors.initials: A
authors.lastname: Pingoud
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaia
mesh_terms: D001391:Azo Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000235:genetics
chemical_list: D001391:Azo Compounds; C045297:CCNGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C009850:azobenzene