Reproductive toxicity of acrylamide-treated male rats.
Acrylamide content is elevated in fried, baked and heat-processed starchy foods. The present experiment was conducted to investigate the reproductive toxicity of oral acrylamide in male rats. Thirty weaned SD male rats of 21-day-old were randomly allotted to three groups, and acrylamide was administered to each group at doses of 0, 5 and 10 mg/kg-d for 8 consecutive weeks. The results indicated that the growth of rats treated with acrylamide was retarded (P<0.05), but relative weights of testes and epididymides compared to body weight were not significantly different (P>0.05). Our results also indicate that the epididymal sperm reserves decreased significantly (P<0.05), suggesting partial depletion of germ cells. In addition, histopathologic lesions were also present in the testes of treated rats. Furthermore, distinct expression patterns of sGC heterodimers were observed in this animal model. This may suggest different physiologic roles for sGC subunits in spermiogenesis and steroidogenesis.
Wang
Hao
H
Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Huang
Pan
P
Lie
Tietao
T
Li
Jian
J
Hutz
Reinhold J
RJ
Li
Kui
K
Shi
Fangxiong
F
D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D004822:Epididymis / Q000187:drug effects* / Q000473:pathology; D006128:Growth / Q000187:drug effects; D006162:Guanylate Cyclase / Q000378:metabolism*; D006965:Hyperplasia / Q000139:chemically induced; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D055503:Protein Multimerization; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D000071756:Soluble Guanylyl Cyclase; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood
D018160:Receptors, Cytoplasmic and Nuclear; D020106:Acrylamide; D013739:Testosterone; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase