A nanobiosensor to detect single hybridization events.
An economical nanoarray method to electrically detect hybridization events is demonstrated. As a proof of concept, we fabricated a sensor for DNA sequencing, in which targets are oligonucleotides conjugated to gold nanoparticles. As a consequence of target-probe binding events, a conductive bridge forms between two electrodes, resulting in a quantized change in conductivity. This enables a robust detection of a few (down to single) hybridization events and can be potentially applied also to other binding events (like specific interactions between proteins, antibodies, ligands and receptors). Moreover, target amplification techniques (such as PCR) are no longer necessary.
Maruccio
Giuseppe
G
National Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, Università del Salento, IIT-Research Unit, v. Arnesano 16, I-73100 Lecce, Italy. giuseppe.maruccio@unisalento.it
Primiceri
Elisabetta
E
Marzo
Pasquale
P
Arima
Valentina
V
Della Torre
Antonio
A
Rinaldi
Ross
R
Pellegrino
Teresa
T
Krahne
Roman
R
Cingolani
Roberto
R
D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D006046:Gold; D053768:Metal Nanoparticles; D036103:Nanotechnology / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods
D006046:Gold; D004247:DNA