title: A rapid and economic in-house DNA purification method using glass syringe filters.
abstract: BACKGROUND
  Purity, yield, speed and cost are important considerations in plasmid purification, but it is difficult to achieve all of these at the same time. Currently, there are many protocols and kits for DNA purification, however none maximize all four considerations.
  
  
  RESULTS
  We now describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters. Plasmid yield and quality as determined by enzyme digestion and transfection efficiency were equivalent to the expensive commercial kits. Importantly, the time required for purification was much less than that required using a commercial kit.
  
  
  CONCLUSIONS
  This method provides DNA yield and quality similar to that obtained with commercial kits, but is more rapid and less costly.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA. kyc8402@ucla.edu
authors.forename: Yun-Cheol
authors.initials: YC
authors.lastname: Kim
authors.forename: Sherie L
authors.initials: SL
authors.lastname: Morrison
mesh_terms: D001419:Bacteria / Q000235:genetics; D002460:Cell Line; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005374:Filtration / Q000295:instrumentation*; D005821:Genetic Techniques / Q000191:economics; D005898:Glass; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013594:Syringes; D014162:Transfection
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic