Correlation of expression of human arrest-defective-1 (hARD1) protein with breast cancer.
Human arrest-defective-1 (hARD1) was reported to be important in regulating cell cycle and promoting lung cancer cell proliferation. Here we have investigated the correlation between hARD1 and breast cancer. Analysis with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry (FCM) demonstrated that overexpression of hARD1 was associated with increased proliferation of MCF-7 cell, a human breast cancer cell line. Western blotting and immunohistochemical staining assay showed that hARD1 presented higher in breast cancer tissue than the adjacent tissue; accumulation of hARD1 protein was higher in 86% (37/43) of breast cancer, far more than noncancer samples. Our results suggest that hARD1 might play an important role in breast cancer carcinogenesis.
Yu
Min
M
Laboratory of Biochemistry and Molecular Biology, School of Life Science, Yunnan University, Kunming, China.
Ma
MingXing
M
Huang
Chao
C
Yang
Hui
H
Lai
JianHua
J
Yan
Shan
S
Li
Lin
L
Xiang
MingJun
M
Tan
DeYong
D
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016373:DNA, Antisense; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D063209:N-Terminal Acetyltransferase A; D063213:N-Terminal Acetyltransferase E; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D016373:DNA, Antisense; D000123:Acetyltransferases; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; D063213:N-Terminal Acetyltransferase E