Effect of siRNA-mediated silencing of Bmi-1 gene expression on HeLa cells.
B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) is highly expressed in several malignant tumors and its expression level is positively correlated with tumor invasion, distant metastasis, prognosis, and recurrence. In the present study, the biological effect of small interfering RNA (siRNA) that targeted Bmi-1 expression was studied in human cervical carcinoma cell line HeLa cells. Bmi-1 siRNA inhibited the expression of Bmi-1 at the mRNA and protein levels in HeLa cells, which significantly affected proliferation, colony formation, and migration of HeLa cells in vitro and in vivo. Therefore, silencing Bmi-1 may be a potential therapeutic option for the management of some human cancers.
Jiang
Yue
Y
Department of Laboratory Hematology, College of Laboratory Medicine of Dalian Medical University, Dalian, China.
Su
Benli
B
Meng
Xiuxiang
X
Liu
Chunqing
C
Liu
Ben
B
Liu
Dandan
D
Fan
Ying
Y
Yang
Hong
H
D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D016193:G1 Phase; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012097:Repressor Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
C069077:BMI1 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1