title: Role of immunofluorescence and electron microscopy in the evaluation of renal biopsies in nephrotic syndrome in a developing country.
abstract: To determine the role of immunofluorescence (IF) and electron microscopy (EM) in the evaluation of renal biopsies in a developing country, the authors carried out a study in 200 patients with nephrotic syndrome. Renal biopsies were studied by light microscopy, IF, and EM. IF study was useful in all, being essential in 23.5% and helpful in remaining cases. EM was useful in 94.5% cases, being essential in 43% and helpful in 51.5% cases. The results demonstrate that IF and EM are essential in the evaluation of renal biopsies in nephrotic syndrome and these should be employed in the pathologic evaluation of renal biopsies.
authors.affiliation: Department of Histopathology, Sindh Institute of Urology and Transplantation (SIUT), Karachi, Pakistan. drmubaraksiut@yahoo.com
authors.forename: Muhammed
authors.initials: M
authors.lastname: Mubarak
authors.forename: Javed Iqbal
authors.initials: JI
authors.lastname: Kazi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003906:Developing Countries; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D007668:Kidney / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000706:statistics & numerical data*; D008856:Microscopy, Fluorescence / Q000706:statistics & numerical data*; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000473:pathology*; D010154:Pakistan; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate