title: Characterisation of ceftiofur resistance in swine bacterial pathogens.
abstract: A PCR based method was developed for the identification of ceftiofur resistance genes (bla(CMY-2), bla(TEM-1), and ampC) in swine bacterial pathogens. Using this method, the ceftiofur resistant (n=76) and susceptible (n=45) strains of Bordetella bronchiseptica, Salmonella spp., Escherichia coli, and Pasteurella multocida were screened for the presence of these three genes. The resistant genes were detected in 70% (bla(TEM-1)), 68% (bla(CMY-2)) and 45% (ampC) of the resistant isolates and in 18% (bla(TEM-1)), 27% (bla(CMY-2)), and 36% (ampC) of the susceptible isolates. Results obtained in the present study showed widespread distribution of these three resistance genes in ceftiofur-resistant swine pathogens. It was also observed that more pathogens are acquiring these resistance genes.
authors.affiliation: Department of Veterinary Population Medicine, 1333 Gortner Avenue, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA. chand062@umn.edu
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Chander
authors.forename: Simone
authors.initials: S
authors.lastname: Oliveira
authors.forename: Sagar M
authors.initials: SM
authors.lastname: Goyal
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016950:Bordetella bronchiseptica / Q000187:drug effects; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D016979:Pasteurella multocida / Q000187:drug effects; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012475:Salmonella / Q000187:drug effects; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy; D018440:beta-Lactam Resistance; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D047090:beta-Lactams; C053503:ceftiofur