title: The Trichoplusia ni cell line MSU-TnT4 does not harbor  a latent nodavirus.
abstract: MSU-TnT4 (TnT4) cells, a newly established Trichoplusia ni cell line, was examined for the presence of a latent nodavirus, as had been described for another T. ni cell line, BTI-TN-5B1-4 (Hi5) cells. Reverse transcriptase polymerase chain reaction using nodavirus-specific primers did not detect virus genomic RNA in TnT4 cells. Transmission electron microscopy of recombinant baculovirus-infected TnT4 cells showed no evidence of latent nodavirus activation. Nodavirus particles were not detected in density gradients of baculovirus-infected TnT4 cell lysates or cell supernatants. The same methods confirmed the presence of a latent nodavirus in Hi5 cells.
authors.affiliation: Department of Entomology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Fengrui
authors.initials: F
authors.lastname: Zhang
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Thiem
mesh_terms: D000818:Animals; D002460:Cell Line; D016679:Genome, Viral / Q000235:genetics; D009036:Moths / Q000166:cytology* / Q000648:ultrastructure / Q000821:virology*; D030381:Nodaviridae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure; D017735:Virus Latency
chemical_list: D012367:RNA, Viral