Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins.
Several peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors.
Zheng
Zhenhua
Z
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, The Key Laboratory of Education Minister for Cell Biology and Tumor Cell Engineering of Xiamen University, Xiamen University, 361005 Xiamen, People's Republic of China.
Luo
Wenxin
W
Song
Huijuan
H
Chen
Yingwei
Y
Xiong
Junhui
J
Chen
Yixin
Y
Gu
Ying
Y
Chen
Honglin
H
Shih
J Wai-Kuo
JW
Zhang
Jun
J
Xia
Ningshao
N
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010455:Peptides; D011993:Recombinant Fusion Proteins