Involvement of Src tyrosine kinase in Escherichia coli invasion of human brain microvascular endothelial cells.
Invasion of brain microvascular endothelial cells is a prerequisite for successful crossing of the blood-brain barrier by Escherichia coli (E. coli), but the underlying mechanism remains unclear. Here we showed activation of Src tyrosine kinase in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC). E. coli invasion of HBMEC and the E. coli-induced rearrangement of actin filaments were blocked by Src inhibitors. Overexpression of dominant-negative Src in HBMEC significantly attenuated E. coli invasion and the concomitant actin filaments rearrangement. Furthermore, E. coli K1-triggered phosphatidylinositol 3-kinase (PI3K) activation in HBMEC was effectively blocked by Src inhibitors and dominant-negative Src. These results demonstrated the involvement of Src and its interaction with PI3K in E. coli K1 invasion of HBMEC.
Liu
Wei
W
Department of Developmental Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Heping Distric, Shenyang, China.
Zhao
Wei-Dong
WD
Yan
Jin-Chun
JC
Ren
Zhi-Yuan
ZY
Fang
Wen-Gang
WG
Zhu
Li
L
Shang
De-Shu
DS
Chen
Yu-Hua
YH
D000199:Actins / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000201:enzymology / Q000382:microbiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism / Q000382:microbiology; D004730:Endothelium, Vascular / Q000201:enzymology / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000201:enzymology*; D006801:Humans; D055806:Microvessels / Q000201:enzymology / Q000382:microbiology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000199:Actins; D019869:Phosphatidylinositol 3-Kinases; D019061:src-Family Kinases