Isolation and characterization of Candida kefyr orotidine-5'-phosphate decarboxylase (URA3) gene.
Candida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5'-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues.
Tsang
Paul Wai-Kei
PW
Oral BioSciences, Faculty of Dentistry, University of Hong Kong.
Wong
Kin-Sing
KS
Chu
Jennifer Ka-Man
JK
D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny
D005656:Fungal Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase