Evaluation of an enterocin AS-48 enriched bioactive powder obtained by spray drying.
Enterocin AS-48 is a cationic cyclic bacteriocin produced by Enterococcus faecalis with broad bactericidal activity. Currently we are assaying the efficacy of AS-48 as biopreservative in foods. In this work we have applied the spray drying process to different AS-48 liquid samples to obtain active dried preparations. We have also assayed different methods, heat, UV irradiation and filtration, to inactivate/remove the AS-48 producer cells from the samples. Best results were obtained for the sample from CM-25 cation exchange, for which it was also possible to completely eliminate/inactivate the producer cells by heat or UV irradiation without loss of activity. When added at 0.016% or 5% to Brain Heart Infusion broth or to skim milk, respectively, the AS-48 powder caused early and complete inactivation of Listeria monocytogenes. A partial inhibition of Staphylococcus aureus was achieved in broth and in skim milk supplemented with 2.5% and 10% AS-48 powder, respectively.
Ananou
S
S
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuentenueva s/n, 19071 Granada, Spain.
Muñoz
A
A
Martínez-Bueno
M
M
González-Tello
P
P
Gálvez
A
A
Maqueda
M
M
Valdivia
E
E
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000378:metabolism / Q000528:radiation effects; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000378:metabolism / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D005520:Food Preservatives; C466236:enterocin AS-48, Enterococcus faecalis