Both GABAA and GABAB receptors mediate vagal inhibition in nucleus tractus solitarii neurones in anaesthetized rats.
GABA receptors in the nucleus tractus solitarius (NTS) are known to play an important role in the mediation/modulation of various cardiovascular/respiratory functions. Vagal afferent activation of these neurones usually evokes an initial excitation followed by a long lasting inhibition. The present study examines the role of GABA(B) as well as GABA(A) receptors in the mediation of this inhibition in anaesthetized rats, using CGP 35348 and bicuculline, respective antagonists at these receptors, applied topically or by ionophoresis. Bicuculline delayed the onset and reduced the duration of this inhibition. The duration of this inhibition was further and significantly decreased when CGP 35348 was administered along with bicuculline but the delay in onset was unaffected. CGP 35348 application alone, had no effect on the vagal afferent-evoked inhibition. From intracellular recordings the early and late components of this inhibition were found to have reversal potentials close to E(Cl) and E(K), respectively. Therefore it is concluded that this inhibition is mediated by both GABA(A) and GABA(B) receptors, although GABA(B) mediated inhibition can only be unmasked when GABA(A) receptors are blocked.
Wang
Yun
Y
Institutes of Brain Science and State Key Laboratory for Medical Neurobiology, Fudan University, Shanghai 200032, China. yunwang@fudan.edu.cn <yunwang@fudan.edu.cn>
Jordan
David
D
Ramage
Andrew G
AG
D000279:Administration, Cutaneous; D000344:Afferent Pathways / Q000187:drug effects; D000758:Anesthesia; D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology; D018756:GABA Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D007478:Iontophoresis; D008297:Male; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000502:physiology*; D018080:Receptors, GABA-B / Q000502:physiology*; D017552:Solitary Nucleus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D013997:Time Factors; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
D018756:GABA Antagonists; D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D009943:Organophosphorus Compounds; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; C066430:CGP 35348; D001640:Bicuculline