Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids.
To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.
Zhan
Z J
ZJ
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China.
Zhou
Z G
ZG
Shan
W G
WG
D004795:Enzyme Stability; D006358:Hot Temperature; D006801:Humans; D019787:Linoleic Acid / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*; D017962:alpha-Linolenic Acid / Q000737:chemistry*
D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D013482:Superoxide Dismutase