title: Hands-free sample preparation platform for nucleic acid analysis.
abstract: A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.
authors.affiliation: Institut für Mikrotechnik Mainz, Carl-Zeiss-Strasse 18-20, 55129, Mainz, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Baier
authors.forename: T E
authors.initials: TE
authors.lastname: Hansen-Hagge
authors.forename: R
authors.initials: R
authors.lastname: Gransee
authors.forename: A
authors.initials: A
authors.lastname: Crombé
authors.forename: S
authors.initials: S
authors.lastname: Schmahl
authors.forename: C
authors.initials: C
authors.lastname: Paulus
authors.forename: K S
authors.initials: KS
authors.lastname: Drese
authors.forename: H
authors.initials: H
authors.lastname: Keegan
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.forename: J J
authors.initials: JJ
authors.lastname: O'Leary
authors.forename: L
authors.initials: L
authors.lastname: Furuberg
authors.forename: L
authors.initials: L
authors.lastname: Solli
authors.forename: P
authors.initials: P
authors.lastname: Grønn
authors.forename: I M
authors.initials: IM
authors.lastname: Falang
authors.forename: A
authors.initials: A
authors.lastname: Karlgård
authors.forename: A
authors.initials: A
authors.lastname: Gulliksen
authors.forename: F
authors.initials: F
authors.lastname: Karlsen
mesh_terms: D045744:Cell Line, Tumor; D004867:Equipment Design; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000191:economics; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014626:Vaginal Smears / Q000191:economics / Q000295:instrumentation*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral