title: Preparation of EGFR monoclonal antibody conjugated nanoparticles and targeting to hepatocellular carcinoma.
abstract: This study aims to determine the sensitivity, specificity and accuracy of epidermal growth factor receptor monoclonal antibody (EGFRmAb) modified poly(lactic acid-co-l-lysine) nanoparticles (PLA-PLL-EGFRmAb) NPs delivery system to EGFR positive cancer cells. In the study, a new PLA-PLL-EGFRmAb NPs was prepared. The cellular cytotoxicity, cellular uptake, and the targeted effect for hepatocellular carcinoma of PLA-PLL-EGFRmAb NPs were investigated. In vitro, the findings of Flow cytometry and Confocal Laser scanning Biological Microscopy showed that PLA-PLL-EGFRmAb NPs can bind to hepatocellular carcinoma cells and were uptaken effectively. In vivo in the SMMC-7721 xenograft mouse model, PLA-PLL-EGFRmAb NPs could target to the tumor effectively, which demonstrated a better targeting. These results showed that the PLA-PLL-EGFRmAb NPs have the potential to be used as a target delivery carrier for tumor therapies.
authors.affiliation: State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 200051, People's Republic of China.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Liu
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Li
authors.forename: Mingjie
authors.initials: M
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Li
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Wang
authors.forename: Yourong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology*; D045744:Cell Line, Tumor; D004339:Drug Compounding / Q000379:methods; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D053769:Nanocapsules / Q000737:chemistry* / Q000627:therapeutic use*
chemical_list: D000911:Antibodies, Monoclonal; D053769:Nanocapsules; C541485:EGFR protein, mouse; D066246:ErbB Receptors