Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli.
We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.
Andreessen
Björn
B
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany.
Lange
Alvin Brian
AB
Robenek
Horst
H
Steinbüchel
Alexander
A
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D010957:Plasmids; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011995:Recombination, Genetic
D054813:Polyhydroxyalkanoates; D005990:Glycerol