Determination of tocopherols and tocotrienols in vegetable oils by nanoliquid chromatography with ultraviolet-visible detection using a silica monolithic column.
A method for the determination of tocopherols and tocotrienols in vegetable oils by nanoliquid chromatography with UV-vis detection has been developed. The separation of tocopherols was optimized in terms of mobile phase composition on the basis of the best compromise between efficiency, resolution, and analysis time. The optimal conditions were achieved using a C18 silica monolithic column (150 mm x 0.1 mm) with an isocratic elution of acetonitrile/methanol/water (acidified with 0.2% acetic acid) at a flow rate of 0.5 microL min(-1), giving a total analysis time below 18 min. The method has been applied to the quantification of tocopherols and tocotrienols present in several vegetable oils with different botanical origins.
Cerretani
Lorenzo
L
Dipartimento di Scienze degli Alimenti, Università di Bologna, I-47023 Cesena, FC, Italy.
Lerma-García
María Jesús
MJ
Herrero-Martínez
José Manuel
JM
Gallina-Toschi
Tullia
T
Simó-Alfonso
Ernesto Francisco
EF
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010938:Plant Oils / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D024505:Tocopherols / Q000032:analysis*; D024508:Tocotrienols / Q000032:analysis*
D010938:Plant Oils; D024508:Tocotrienols; D024505:Tocopherols