Cloning and characterization of cytidine monophosphate-3-deoxy-d-manno-octulosonate synthetase from Arabidopsis thaliana.
The function and metabolic pathway of 3-deoxy-d-manno-octulosonate (KDO) are unclear in plants although it is an essential component in plant cell wall. Here we cloned and characterized a putative Arabidopsis thaliana cytidine monophosphate-KDO synthetase to understand synthetic pathways of KDO. It showed a ubiquitous expression, the activity at an optimal pH of 8.0, and a requirement of Mg2+.
Misaki
Ryo
R
The International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565-0871, Japan.
Kajiura
Hiroyuki
H
Fujii
Kenji
K
Fujiyama
Kazuhito
K
Seki
Tatsuji
T
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology; D003001:Cloning, Molecular; D003568:Cytidine Monophosphate / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013400:Sugar Acids / Q000737:chemistry / Q000378:metabolism
D029681:Arabidopsis Proteins; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D009713:Nucleotidyltransferases; C548443:cytidine monophosphate-3-deoxymannooctulosonate synthetase, Arabidopsis; D008025:Ligases; D003568:Cytidine Monophosphate