Crystallization and preliminary crystallographic analysis of gallate dioxygenase DesB from Sphingobium sp. SYK-6.
Gallate dioxygenase (DesB) from Sphingobium sp. SYK-6, which belongs to the type II extradiol dioxygenase family, was purified and crystallized using the hanging-drop vapour-diffusion method. Two crystal forms were obtained. The form I crystal belonged to space group C2, with unit-cell parameters a = 136.2, b = 53.6, c = 55.1 angstrom, beta = 112.8 degrees, and diffracted to 1.6 angstrom resolution. The form II crystal belonged to space group P2(1), with unit-cell parameters a = 56.2, b = 64.7, c = 116.1 angstrom, beta = 95.1 degrees, and diffracted to 1.9 angstrom resolution. A molecular-replacement calculation using LigAB as a search model yielded a satisfactory solution for both crystal forms.
Sugimoto
Keisuke
K
Department of Materials Chemistry, Asahikawa National College of Technology, Shunko-dai, Asahikawa, Hokkaido, Japan.
Yamamoto
Yoshihiro
Y
Antoni
Siswanto
S
Senda
Miki
M
Kasai
Daisuke
D
Masai
Eiji
E
Fukuda
Masao
M
Senda
Toshiya
T
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics; D020578:Sphingomonas / Q000201:enzymology*; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D010105:Oxygenases; C439587:extradiol dioxygenase