Involvement of HSP10 during the ovarian follicular development of polycystic ovary syndrome: Study in both human ovaries and cultured mouse follicles.
OBJECTIVE
To study the possible roles of HSP10 in the follicular development.


METHODS
In this study, we examined the expression of HSP10 during the follicular development in human ovaries and cultured mouse follicles as well as its functional relevance with PCOS. Ovary tissues from normal adults (n = 3) were obtained with consents. Mouse early antral follicles (diameter: 220-250 mum) were cultured for 3 days in vitro. Western Blot and immunohistochemistry were used to determine the HSP10 expression and localisation during follicular development in vivo and in vitro.


RESULTS
HSP10 protein was detected only in oocytes from human preantral follicles, whereas in antral follicles, it was localised in oocytes, granulosa cells, theca cells and stroma cells. Furthermore, in cultured mouse antral follicles, a similar trend of HSP10 expression during follicle development was observed.


CONCLUSIONS
HSP10 expression was increased as larger area and higher level of density during follicular development both in human and mouse follicles cultured in vitro. Our previous studies showed that HSP10 was highly expressed in normal ovaries compared with those from PCOS. The mouse early antral follicle culture approach may help to understand the role of HSP10 in pathophysiological development of PCOS.
Fan
Lu
L
Jiangsu Province Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
Fan
Lu
L
Ling
Jing
J
Ma
Xiang
X
Cui
Yu-Gui
YG
Liu
Jia-Yin
JY
D000328:Adult; D000818:Animals; D018835:Chaperonin 10 / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D006080:Ovarian Follicle / Q000254:growth & development* / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism; D046509:Tissue Culture Techniques; D055815:Young Adult
D018835:Chaperonin 10; D004958:Estradiol