Bacterial leakage evaluation of root canals filled with different endodontic sealers.
OBJECTIVE
The objective of this study was to evaluate the sealing ability of AH Plus, Epiphany, Acroseal, Endofill, and Polifil after active lateral condensation technique, by using a bacterial test, during 64 days.


METHODS
One hundred bovine incisors were cleaned and shaped; then they were filled with the endodontic sealers and adapted into a microcentrifuge tube. The setup root/microcentrifuge tube was added to glass flasks containing Brain Heart Infusion broth. A culture of Enterococcus faecalis was inserted into the upper chamber of each assembly. Daily leakage was evaluated through the broth turbidity.


RESULTS
The results were submitted to statistical analysis (Kaplan-Meier method, Kruskal-Wallis and Dunn tests).


CONCLUSIONS
AH Plus and Endofill had the worst sealing ability when compared with Polifil, which showed the least leakage. Acroseal and Epiphany showed a tendency toward having an intermediate behavior; however, there was no significant difference among Acroseal, Epiphany, and the other sealers.
Pinheiro
Cláudia Ramos
CR
Department of Restorative Dentistry, Araraquara Dental School, São Paulo State University, Araraquara, São Paulo, Brazil. claudiaendo@gmail.com
Guinesi
Adriana Simionatto
AS
de Camargo
Ericson Janólio
EJ
Pizzolitto
Antônio Carlos
AC
Filho
Idomeo Bonetti
IB
D000818:Animals; D002417:Cattle; D003763:Dental Leakage / Q000517:prevention & control*; D018772:Dental Marginal Adaptation; D003786:Dental Pulp Cavity / Q000382:microbiology / Q000601:surgery; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004853:Epoxy Resins / Q000737:chemistry / Q000145:classification; D007180:Incisor; D008422:Materials Testing; D012387:Root Canal Filling Materials / Q000737:chemistry* / Q000145:classification; D012389:Root Canal Obturation / Q000379:methods*; D018709:Statistics, Nonparametric
D004853:Epoxy Resins; D012387:Root Canal Filling Materials