Synthesis of coenzyme Q10 and beta-carotene by yeasts isolated from antarctic soil and lichen in response to ultraviolet and visible radiations.
The effect of different doses of visible (Vis), ultraviolet-capital A, Cyrillic (UVA), and mixed light (UVA + Vis) upon coenzyme Q(10) (CoQ(10)) and beta-carotene synthesis and biomass yield by the Sporobolomyces salmonicolor AL(1), Cryptococcus albidus AS(55), Cryptococcus laurentii AS(56), and C. laurentii AS(58) strains isolated from Antarctic samples was investigated. The beta-carotene concentration in the red strain biomass increased by 52% under irradiation with 11 J/cm(2) Vis, and the CoQ(10) concentration rose by 37% in relation to the control quantity obtained through dark cultivation. Under irradiation with 6 J/cm(2) UVA, the S. salmonicolor AL(1) strain synthesized 15% more beta-carotene; C. albidus AS(55), 22%; C. laurentii AS(56), 44%; and C. laurentii AS(58), 35% in relation to the control quantity. Irradiation with a low UVcapital A, Cyrillic + Vis dose significantly stimulated beta-carotene biosynthesis by the strains of the Cryptococcus genus (87%, 138%, and 100%), whereas S. salmonicolor AL(1) increased the beta-carotene content to a smaller degree (55%). Higher doses of all three irradiation types inhibited beta-carotene accumulation. Vis suppressed CoQ(10) biosynthesis in the Cryptococcus strains, whereas UVcapital A, Cyrillic and UVcapital A, Cyrillic + Vis inhibited it in all four strains. The S. salmonicolor AL(1) strain pre-treated with 0.02 J/cm(2) UVA synthesized twice as much CoQ(10) and beta-carotene when cultivated in the presence of Vis light in an 11-J/cm(2) dose.
Dimitrova
Stela
S
Department of Chemistry and Biochemistry, Medical University, V. Aprilov Blvd 15A, Plovdiv 4002, Bulgaria. szd@abv.bg
Pavlova
Kostantsa
K
Lukanov
Ludmil
L
Zagorchev
Plamen
P
D000864:Antarctic Regions; D001487:Basidiomycota / Q000378:metabolism / Q000528:radiation effects; D003454:Cryptococcus / Q000378:metabolism / Q000528:radiation effects; D008011:Lichens / Q000382:microbiology*; D012988:Soil Microbiology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D014466:Ultraviolet Rays; D015003:Yeasts / Q000378:metabolism* / Q000528:radiation effects*; D019207:beta Carotene / Q000096:biosynthesis*
D019207:beta Carotene; D014451:Ubiquinone; C024989:coenzyme Q10