title: Identification of the non-structural influenza A viral protein NS1A as a bona fide target of the Small Ubiquitin-like MOdifier by the use of dicistronic expression constructs.
abstract: The cellular SUMOylation system affects the function of numerous viral proteins. Hence, the identification of novel viral targets for the Small Ubiquitin-like MOdifier (SUMO) is key to our understanding of virus-host interactions. The data obtained in this study demonstrate that the non-structural influenza A viral protein NS1A is an authentic SUMO target through the use of a dicistronic expression plasmid containing SUMO (the modifier) and Ubc9 (the SUMO-conjugating enzyme) separated by an Internal Ribosomal Entry Site (IRES). This dual expression plasmid produces a robust increase in cellular SUMOylation, therefore facilitating the characterization of cellular and viral SUMO targets. The identification of NS1A as a bona fide SUMO target suggests, for the first time, a role for SUMOylation during influenza virus infection.
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, United States.
authors.forename: Sangita
authors.initials: S
authors.lastname: Pal
authors.forename: Juan M
authors.initials: JM
authors.lastname: Rosas
authors.forename: Germán
authors.initials: G
authors.lastname: Rosas-Acosta
mesh_terms: D005822:Genetic Vectors; D054884:Host-Pathogen Interactions; D006801:Humans; D009975:Orthomyxoviridae / Q000502:physiology*; D010957:Plasmids; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism; D054875:Ubiquitination; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: C085820:INS1 protein, influenza virus; D025842:SUMO-1 Protein; D025801:Ubiquitin; D017361:Viral Nonstructural Proteins; D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9