title: Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome.
abstract: The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 degrees C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Kien Xuan
authors.initials: KX
authors.lastname: Ngo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Umakoshi
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Ishii
authors.forename: Huong Thi
authors.initials: HT
authors.lastname: Bui
authors.forename: Toshinori
authors.initials: T
authors.lastname: Shimanouchi
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kuboi
mesh_terms: D000327:Adsorption; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002942:Circular Dichroism; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010087:Oxides / Q000378:metabolism; D010539:Permeability; D010713:Phosphatidylcholines / Q000737:chemistry; D012995:Solubility; D013305:Streptomyces griseus / Q000201:enzymology*; D013312:Stress, Physiological
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D008567:Membranes, Artificial; D010087:Oxides; D010713:Phosphatidylcholines; D006861:Hydrogen Peroxide; D006026:Glycoside Hydrolases; C019868:chitosanase; C028694:1-palmitoyl-2-oleoylphosphatidylcholine