An antibody surface for selective neuronal cell attachment.
An optimal surface for culturing human embryonic stem cell (hESC)-derived neuronal cells is of high interest. In this study, a specific antibody to a neural cell adhesion molecule (NCAM) was immobilised on a solid surface of polystyrene and used as a selective matrix for culturing of hESC-derived neuronal cells. Thereafter, hESC-derived neurospheres were seeded on the matrix. The neurospheres did not attach to the NCAM antibody containing matrix whereas individual neuronal cells did. The neuronal cell attachment was depended on the NCAM antibody concentration. The neuronal cells were viable on the NCAM antibody containing matrix during an 8 day follow-up and exhibited typical bipolar morphology of immature neurons. Specific binding of the NCAM antigen to an immunoglobulin-polymer coated surface was verified by surface plasmon resonance (SPR) measurements. This study is to our knowledge the first demonstrating the use of an antibody layer as a selective surface for hESC-derived neuronal cells.
Auer
Sanna
S
VTT Technical Research Centre of Finland, P.O. Box 1300, FIN-33101 Tampere, Finland. Sanna.Auer@vtt.fi
Lappalainen
Riikka S
RS
Skottman
Heli
H
Suuronen
Riitta
R
Narkilahti
Susanna
S
Vikholm-Lundin
Inger
I
D000906:Antibodies / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D019006:Neural Cell Adhesion Molecules / Q000276:immunology*; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry; D011485:Protein Binding / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000379:methods
D000906:Antibodies; D003470:Culture Media; D007136:Immunoglobulins; D019006:Neural Cell Adhesion Molecules; D011137:Polystyrenes