Chiral and structural analysis of biomolecules using mass spectrometry and ion mobility-mass spectrometry.
This report describes the strategies for gas-phase chiral and structural characterization of biomolecules using mass spectrometry (MS) and ion mobility-MS (IM-MS) techniques. Because both MS and IM-MS do not directly provide chiral selectivity, methodologies for adding a chiral selector are discussed in the context of (i) host-guest (H-G) associations, (ii) diastereomeric collision-induced dissociation (CID) methods, (iii) ion-molecule reactions, and (iv) the kinetic method. MS techniques for the analysis of proteins and protein complexes are briefly described. New advances in performing rapid 2D gas-phase separations on the basis of IM-MS are reviewed with a particular emphasis on the different forms of IM instrumentation and how they are used for chiral and/or structural biomolecular studies. This report is not intended to be a comprehensive review of the field, but rather to underscore the contemporary techniques that are commonly or increasingly being used to complement measurements performed by chiroptical methodologies.
Enders
Jeffrey R
JR
Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
McLean
John A
JA
D002241:Carbohydrates / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013237:Stereoisomerism; D013816:Thermodynamics
D002241:Carbohydrates; D007477:Ions; D008055:Lipids; D009711:Nucleotides; D010455:Peptides