[Effects of duodenal-jejunal bypass and sleeve gastrectomy on the expression of liver glucokinase in diabetic rats].
OBJECTIVE
To investigate the effects of duodenal-jejunal bypass(DJB) and sleeve gastrectomy(SG) on the expression of liver glucokinase(GCK) in diabetic rats.


METHODS
Animal models of Goto-Kakizaki rats and Sprague-Dawley rats were established by DJB and SG. Results of fasting glycemia and insulin were compared. Liver tissue was harvested 8 weeks postoperatively.Quantitative real-time PCR and Western blot were used to detect liver GCK mRNA and protein expression after operation.


RESULTS
Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined 3 day and 1, 2, 4, 6, 8 weeks postoperatively(all P <0.01), while Sham group only dropped 3 day and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively(P >0.05). Fasting plasma glucose levels of each group in SD rats did not change after operation. In GK rats, GCK mRNA level (1.45 +/-0.29) and protein expression (494.25 +/-30.25) after DJB were higher than Sham group (1.05 +/-0.19 and 409.13 +/-26.86) and control group (1.04 +/-0.17 and 404.75 +/-30.90). GCK mRNA level and protein expression after SG were 0.65 +/-0.25 and 345.25 +/-28.13 respectively, which were significantly lower than those in control group(all P <0.01). All the groups in SD rats experienced similar GCK expression change.


CONCLUSIONS
Both DJB and SG can decrease the plasma glucose levels of GK rats, while there are different effects on the expression of liver GCK.
Zhou
Dong-lei
DL
Department of Minimal Invasive Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
Zhang
Ding-yu
DY
Hu
Xu-guang
XG
Chen
Dan-lei
DL
Ke
Zhong-wei
ZW
Yin
Kai
K
Li
Ji-hui
JH
Zheng
Cheng-zhu
CZ
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000601:surgery; D013505:Digestive System Surgical Procedures / Q000379:methods; D004386:Duodenum / Q000601:surgery; D005743:Gastrectomy; D005941:Glucokinase / Q000378:metabolism*; D007583:Jejunum / Q000601:surgery; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D005941:Glucokinase