title: Satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors.
abstract: The coat protein of satellite panicum mosaic virus (SPCP) is known to effectively protect its cognate RNA from deleterious events, and here, we tested its stabilizing potential for heterologous virus-based gene vectors in planta. In support of this, a Potato virus X (PVX) vector carrying the SPMV capsid protein (PVX-SPCP) gene was stable for at least three serial systemic passages through Nicotiana benthamiana. To test the effect of SPCP in trans, PVX-SPCP was co-inoculated onto N. benthamiana together with a Tomato bushy stunt virus (TBSV) vector carrying a green fluorescent protein (GFP) gene that normally does not support systemic GFP expression. In contrast, co-inoculation of TBSV-GFP plus PVX-SPCP resulted in GFP accumulation and concomitant green fluorescent spots in upper, non-inoculated leaves in a temperature-responsive manner. These results suggest that the multifaceted SPMV CP has intriguing effects on virus-host interactions that surface in heterologous systems.
authors.affiliation: Department of Plant Pathology and Microbiology, 2132 TAMU, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Anthany L
authors.initials: AL
authors.lastname: Everett
authors.forename: Herman B
authors.initials: HB
authors.lastname: Scholthof
authors.forename: Karen-Beth G
authors.initials: KB
authors.lastname: Scholthof
mesh_terms: D036022:Capsid Proteins / Q000502:physiology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D009029:Mosaic Viruses / Q000502:physiology*; D008897:Panicum / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D017863:Potexvirus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012526:Satellite Viruses / Q000502:physiology*; D013696:Temperature; D017864:Tombusvirus / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D049452:Green Fluorescent Proteins