Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
Siju
S
S
Indian Institute of Spices Research, Calicut, Kerala, India.
Dhanya
K
K
Syamkumar
S
S
Sasikumar
B
B
Sheeja
T E
TE
Bhat
A I
AI
Parthasarathy
V A
VA
D030024:Curcuma / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D004586:Electrophoresis; D020224:Expressed Sequence Tags; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D018744:DNA, Plant