The probiotic properties of Lactobacillus buchneri P2.
OBJECTIVE
To isolate new lactobacilli strain with cholesterol-lowering effect and analyse its probiotic properties and possible mechanisms of cholesterol removal.


RESULTS
The strain with cholesterol-lowering effect was isolated from pickled juice. The acid and bile tolerance and antimicrobial activity were tested. The free cholalic acid, the cholesterol in supernatant fluid, washing buffer and cell extract, the cholesterol removed by growing, dead and resting cells were quantified. The isolated strain with high cholesterol-reducing rate of 43.95% was identified as Lactobacillus buchneri (Lact. buchneri) P2. It had acid and bile tolerance and antimicrobial activity. Moreover, it could remove cholesterol via coprecipitating with deconjugated bile salts, assimilating and adsorbing by cells. And the assimilation was considered to be the main reason of cholesterol removal.


CONCLUSIONS
The isolated Lact. buchneri P2 showed probiotic properties of cholesterol reduction, acid and bile tolerance and antimicrobial activity and could remove cholesterol via different ways.


CONCLUSIONS
A new strain of Lact. buchneri P2 with efficient cholesterol-reducing ability was isolated to provide species diversity of lactobacilli for functional dairy products. And the possible mechanism of cholesterol removal by Lact. buchneri was discussed.
Zeng
X Q
XQ
Food Science and Nutrition Department, Nanjing Normal University, Nanjing, China.
Pan
D D
DD
Guo
Y X
YX
D000143:Acids / Q000494:pharmacology; D000818:Animals; D000898:Antibiosis; D001647:Bile Acids and Salts / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D019936:Probiotics; D013656:Taurocholic Acid / Q000378:metabolism
D000143:Acids; D001647:Bile Acids and Salts; D013656:Taurocholic Acid; D002784:Cholesterol