Formation of well-oriented microtubules with preferential polarity in a confined space under a temperature gradient.
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
Kakugo
Akira
A
Department of Biological Science, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
Tamura
Yoshiki
Y
Shikinaka
Kazuhiro
K
Yoshida
Momoko
M
Kawamura
Ryuzo
R
Furukawa
Hidemitsu
H
Osada
Yoshihito
Y
Gong
Jian Ping
JP
D016880:Anisotropy; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D032701:Biomimetics; D019277:Entropy; D005898:Glass; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry; D016547:Kinesins / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D008870:Microtubules / Q000737:chemistry* / Q000648:ultrastructure; D013696:Temperature; D014404:Tubulin / Q000737:chemistry*
D014404:Tubulin; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D016547:Kinesins