Interaction of alpha-gliadin with polyanions: design considerations for sequestrants used in supportive treatment of celiac disease.
Copolymers of sodium 4-styrene sulfonate (SS) and hydroxyethyl methacrylate (HEMA) were investigated as sequestrants of alpha-gliadin, a gluten protein, for the treatment of gluten intolerance. The interactions of alpha-gliadin with poly(SS) and poly(HEMA-co-SS) with 9 and 26 mol% SS content were studied at gastric (1.2) and intestinal (6.8) pH using circular dichroism and measurements of turbidity, dynamic light scattering and zeta potential. The interactions and their influence on alpha-gliadin secondary and aggregated structures depended mainly on the ratio of polymer negative and protein positive charges at pH 1.2, and on polymer SS content at polymer concentrations providing in excess of negative charges at either pH. Poly(SS) could not form complex particles with alpha-gliadin in a sufficient excess of negative charges. Copolymerization with HEMA enhanced the formation of complex particles. Poly(HEMA-co-SS) with intermediate SS content was found to be the most effective sequestrant for alpha-gliadin. This study provides insight into design considerations for polymer sequestrants used in the supportive treatment of celiac disease.
Liang
Li
L
Institut de Recherche sur les Nutraceutiques et les Aliments Fonctionnels, Université Laval, Québec, QC, Canada.
Pinier
Maud
M
Leroux
Jean-Christophe
JC
Subirade
Muriel
M
D002446:Celiac Disease / Q000188:drug therapy*; D002942:Circular Dichroism; D015195:Drug Design; D005903:Gliadin / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D015394:Molecular Structure; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D013451:Sulfonic Acids / Q000737:chemistry*
D005903:Gliadin; D008689:Methacrylates; D000071228:Polyelectrolytes; D011108:Polymers; D013451:Sulfonic Acids; C005044:hydroxyethyl methacrylate; C077114:styrenesulfonic acid polymer