title: Nanoparticles for dentine tubule infiltration: an in vitro study.
abstract: Nanosized particles of hydroxyapatite were produced by a hydrothermal synthesis technique. Reaction time and starting pH were varied in order to produce a range of particle dimensions and morphologies. The particles were suspended in alcohol and used for in-vitro trials of dentinal tubule infiltration in etched sections of clinically extracted human molars. Particles were of rod-like morphology, and decreased in aspect ratio as the starting pH was raised. Particles prepared at pH 12 had a mean length of approximately 70 nm and diameter of approximately 30 nm. These showed the most promising infiltration results, with up to 90% of the tubules being fully or partially occluded. Comparisons were made with commercially-available 40 nm spherical silica particles. Information on the depth of infiltration was obtained from sections of dentine prepared using focussed ion beam milling (FIB-SEM).
authors.affiliation: Institute for Materials Research, University of Leeds, Leeds, LS2 9JT7, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Earl
authors.forename: D J
authors.initials: DJ
authors.lastname: Wood
authors.forename: S J
authors.initials: SJ
authors.lastname: Milne
mesh_terms: D003460:Crystallization / Q000379:methods*; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure*; D004058:Diffusion; D017886:Durapatite / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D017886:Durapatite