title: Sequence variation and genetic evolution at the human F12 locus: mapping quantitative trait nucleotides that influence FXII plasma levels.
abstract: The level of Factor XII (FXII) is an important phenotype that exhibits a high genetic component and is associated with thrombotic disease. In a genome-wide linkage scan, we demonstrated that the F12 gene represents a quantitative trait locus (QTL) that influences FXII levels. The current study investigated the genetic architecture of the F12 gene to locate polymorphism(s) responsible for the variation of FXII levels. Re-sequencing of the F12 gene in 40 unrelated individuals (selected from the tails of normal distribution of FXII levels) identified 26 polymorphisms which were genotyped in 398 individuals belonging to 21 families from the GAIT Project. By a measured genotype association analysis, eight of 26 SNPs showed significant P-values less than 10(-5) (after multiple test correction) with FXII levels. In addition, the Bayesian Quantitative Trait Nucleotide method, which infers those polymorphisms most likely to have a direct influence on the trait under study, provided evidence that only rs1801020 variation accounted for the variance attributed to this QTL. Moreover, we have analyzed the evolutionary processes that produced the variation in F12 gene and concluded that is evolutionarily neutral and that the T allele of the rs1801020 appeared approximately 100 000 years ago and spread to most human populations rising to high frequencies by genetic drift. Our study provides a template for future genetic studies of human quantitative traits, as we move beyond QTL localization to the polymorphisms responsible for the variation of important biomedical phenotypes.
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Institute of Evolutionary Biology (UPF-CSIC), Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Calafell
authors.forename: Laura
authors.initials: L
authors.lastname: Almasy
authors.forename: Maria
authors.initials: M
authors.lastname: Sabater-Lleal
authors.forename: Alfonso
authors.initials: A
authors.lastname: Buil
authors.forename: Carolina
authors.initials: C
authors.lastname: Mordillo
authors.forename: Anna
authors.initials: A
authors.lastname: Ramírez-Soriano
authors.forename: Martin
authors.initials: M
authors.lastname: Sikora
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Souto
authors.forename: John
authors.initials: J
authors.lastname: Blangero
authors.forename: Jordi
authors.initials: J
authors.lastname: Fontcuberta
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Soria
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D005174:Factor XII / Q000032:analysis* / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013030:Spain
chemical_list: D005174:Factor XII