The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolor.
Streptomyces coelicolor mutants lacking the zinc-responsive Zur repressor are conditionally defective in sporulation, presumably due to the overexpression of one or more Zur target genes. Gene disruption analyses revealed that deregulation of previously known Zur targets was not responsible for the sporulation phenotype. We used microarrays to identify further Zur targets and discovered that Zur controls a cluster of genes predicted to direct synthesis of an uncharacterized siderophore-related non-ribosomally encoded peptide designated coelibactin. Disruption of a key coelibactin biosynthetic gene suppressed the Zur sporulation phenotype, suggesting that deregulation of coelibactin synthesis inhibits sporulation.
Kallifidas
Dimitris
D
Department of Chemistry and Biochemistry, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Pascoe
Ben
B
Owen
Gillian A
GA
Strain-Damerell
Claire M
CM
Hong
Hee-Jeon
HJ
Paget
Mark S B
MS
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D005810:Multigene Family / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins