Flavones and phenylpropanoids from a sedative extract of Lantana trifolia L.
The flavone glycosides, named scutellarein-7-O-beta-D-apiofuranoside and apigenin-7-O-beta-D-apiofuranosyl-(1-->2)-beta-D-apiofuranoside, and the flavone celtidifoline (5,6,4',5'-tetrahydroxy-7,3'-dimethoxyflavone), along with other 11 known compounds, were isolated from leaves of the ethyl acetate extract of Lantana trifolia L. using step gradient High Speed Countercurrent Chromatography (HSCCC) and High Performance Liquid Chromatography (HPLC), respectively. Their structures were elucidated by spectroscopic methods, including 2D NMR and mass spectrometry (ESI-MS) techniques. The ethanolic and ethyl acetate extracts produced an intense sedative effect in mice, one hour after oral administration of 1 mg/kg. This effect was neither due to a benzodiazepine-like effect of the three flavone derivatives neither of the phenylpropanoids, betonyoside F and verbascoside, that were tested for their affinity for the [3H] flunitrazepam binding sites.
Julião
Lisieux de Santana
Lde S
Universidade Federal do Rio de Janeiro, Programa de Biotecnologia Vegetal, CCS, Bl. K, Rio de Janeiro, RJ, Brazil.
Leitão
Suzana Guimarães
SG
Lotti
Cinzia
C
Picinelli
Anna Lisa
AL
Rastrelli
Luca
L
Fernandes
Patricia D
PD
Noël
François
F
Thibaut
Jean-Pierre Barros
JP
Leitão
Gilda Guimarães
GG
D000818:Animals; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006993:Hypnotics and Sedatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032410:Lantana / Q000737:chemistry*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D020005:Propanols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D047309:Flavones; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D020005:Propanols