Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose.
We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from alpha1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
Dang
Fuquan
F
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Hayashi-cho 2217-14, Takamatsu 761-0395, Japan. fuquan-dang@aist.go.jp
Maeda
Eiki
E
Osafune
Tomo
T
Nakajima
Kazuki
K
Kakehi
Kazuaki
K
Ishikawa
Mitsuru
M
Baba
Yoshinobu
Y
D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D037102:Lectins / Q000737:chemistry*; D009961:Orosomucoid / Q000737:chemistry; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry* / Q000378:metabolism; D010969:Plastics / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D037102:Lectins; D009961:Orosomucoid; D010969:Plastics; D011134:Polysaccharides; D014867:Water; D002482:Cellulose; C002283:hydroxyethylcellulose; D019904:Polymethyl Methacrylate; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase