Levels of plasmacytoid dendritic cells and type-2 myeloid dendritic cells are reduced in peripheral blood of patients with primary Sjogren's syndrome.
OBJECTIVE
Sjögren's syndrome (SS) is a lymphoproliferative autoimmune disease, characterised by dryness of the mouth and eyes. Dendritic cells (DC) are potent antigen-presenting cells crucial for initiating and maintaining primary immune responses. This study quantified interferon-producing plasmacytoid DC (pDC) and two myeloid DC subsets (mDC1 and mDC2) in peripheral blood (PB) from primary SS (pSS) patients and healthy controls.


METHODS
Blood samples from 31 pSS patients and 28 gender and age-matched healthy controls were analysed by flow cytometry using the Miltenyi Blood DC enumeration kit. The presence of pDC in salivary glands (SG) from pSS patients was analysed by immunohistochemistry.


RESULTS
Patients with pSS had significantly less pDC and mDC2 in PB compared with healthy controls. Moreover, pDC are present in SG from patients with pSS.


CONCLUSIONS
Patients with pSS have alterations among DC populations in PB, and pDC are present in the SG, suggesting a potential role of these cells in SS.
Vogelsang
Petra
P
Broegelmann Research Laboratory, The Gade Institute, University of Bergen, Laboratory building, 5th floor, N-5021 Bergen, Norway.
Brun
Johan G
JG
Oijordsbakken
Gunnvor
G
Skarstein
Kathrine
K
Jonsson
Roland
R
Appel
Silke
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology* / Q000276:immunology; D012470:Salivary Glands, Minor / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology*; D055815:Young Adult
D001323:Autoantibodies