Structural-functional characteristics of a new bioregulator isolated from bovine pigmented epithelium tissue.
A new bioregulator operating in ultralow doses corresponding to 10(-17) mg/ml has been isolated from tissue of pigmented epithelium of bovine eyes. It has been established that the functional basis of this bioregulator is a complex of a low molecular weight regulatory peptide (4372 Da) and a modulator consisting of a mixture of proteins with molecular weights of 14.980-66.283 kDa. It has been shown that the regulatory peptide is responsible for membranotropic activity of the bioregulator, and the modulator proteins are responsible for biological action in ultralow doses. The data demonstrate an interrelation between nanocondition of the bioregulator and its ability to show activity in ultralow doses.
Yamskova
V P
VP
Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia. Yamskova-vp@yandex.ru
Skripnikova
V S
VS
Molyavka
A A
AA
Il'ina
A P
AP
Krasnov
M S
MS
Margasyuk
D V
DV
Borisenko
A V
AV
Berezin
B B
BB
Kuznetsova
E S
ES
Buryak
A K
AK
Yamskov
I A
IA
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D010316:Particle Size; D010455:Peptides / Q000737:chemistry / Q000502:physiology*; D011506:Proteins / Q000737:chemistry / Q000502:physiology*; D055213:Retinal Pigment Epithelium / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
D010455:Peptides; D011506:Proteins