Full automation of (68)Ga labelling of DOTA-peptides including cation exchange prepurification.
Here we describe a fully automated approach for the synthesis of (68)Ga-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed (68)Ge as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times.
Ocak
M
M
Clinical Department of Nuclear Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
Antretter
M
M
Knopp
R
R
Kunkel
F
F
Petrik
M
M
Bergisadi
N
N
Decristoforo
C
C
D002411:Cation Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005710:Gallium Radioisotopes / Q000737:chemistry* / Q000302:isolation & purification*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D007553:Isotope Labeling / Q000295:instrumentation*; D010455:Peptides / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000302:isolation & purification; D012371:Robotics / Q000295:instrumentation*
D002411:Cation Exchange Resins; D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D010455:Peptides; D019275:Radiopharmaceuticals; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid