A novel function of red pigment-concentrating hormone in crustaceans: Porcellio scaber (Isopoda) as a model species.
The RP HPLC and LC/MS QTOF analyses of the methanolic CNS extract from isopod crustacean the woodlouse, Porcellio scaber revealed a presence of the red pigment-concentrating hormone (Panbo-RPCH) in this species. It has been shown that this neuropeptide plays a role in mobilization of energy stores: topical treatments of P. scaber individuals by Panbo-RPCH in a concentration 20 pmol/microl increased the level of glucose in haemolymph about 4 times, while the level of trehalose was only doubled. The results demonstrated that glucose was the main carbohydrate mobilized by the Panbo-RPCH treatment: glucose was responsible for about 97% of total carbohydrate increasing. Despite the demonstration of hyperglycaemic activity of Panbo-RPCH, no stimulatory effect of this hormone on the locomotory activity of P. scaber was observed. The present study is the first discovery of an occurrence of Panbo-RPCH and its hyperglycaemic activity in the representative of the isopod crustaceans. The relationship of the function of Panbo-RPCH in P. scaber to the role of this neuropeptide and adipokinetic hormones in insects is discussed.
Zralá
Jana
J
Institute of Entomology, Biology Centre, Academy of Sciences, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
Kodrík
Dalibor
D
Zahradnícková
Helena
H
Zemek
Rostislav
R
Socha
Radomír
R
D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005216:Fat Body / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D007447:Invertebrate Hormones; D033321:Isopoda / Q000737:chemistry* / Q000502:physiology*; D009043:Motor Activity / Q000187:drug effects; D009842:Oligopeptides / Q000008:administration & dosage / Q000032:analysis / Q000502:physiology*; D011761:Pyrrolidonecarboxylic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis; D014199:Trehalose / Q000378:metabolism
D007447:Invertebrate Hormones; D009842:Oligopeptides; C022059:red pigment-concentrating hormone; D014199:Trehalose; D005947:Glucose; D011761:Pyrrolidonecarboxylic Acid