Differential expression of LIM-homeodomain factors in Cajal-Retzius cells of primates, rodents, and birds.
Reelin-expressing Cajal-Retzius (CR) cells are among the earliest generated cells in the mammalian cerebral cortex and are believed to be crucial for both the development and the evolution of a laminated pattern in the pallial wall of the telencephalon. LIM-homeodomain (LIM-hd) transcription factors are expressed during brain development in a highly restricted and combinatorial manner, and they specify regional and cellular identity. We have investigated the expression of the LIM-hd members Lhx1/Lhx2/Lhx5/Lhx6/Lhx9 in the reelin-expressing cells, the pallium, and the regions of origin of CR cells including the cortical hem of 3 amniote species: the mouse, the chick, and the macaque monkey. We found major differences in the combinatorial LIM-hd expression in the marginal zone as well as in the hem. 1) Lhx5 is a "preferential LIM-hd" for CR cells in mammals but not expressed by these cells in chicks. 2) Lhx2 is expressed in the hem of the chick, whereas it is excluded from this region in mouse. 3) Whereas mouse CR cells express Lhx5/Lhx1, their monkey counterparts express 4 of these factors: Lhx1/Lhx2/Lhx5/Lhx9. We discuss our findings in evolutionary terms for the specification of the midline hem and CR cell type and the emergence of the cortical lamination pattern.
Abellan
Antonio
A
Laboratory of Brain Development and Evolution, Department of Experimental Medicine, Faculty of Medicine, University of Lleida, Institute of Biomedical Research of Lleida, 25008 Lleida, Spain.
Menuet
Arnaud
A
Dehay
Colette
C
Medina
Loreta
L
Rétaux
Sylvie
S
D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D060850:LIM-Homeodomain Proteins; D008252:Macaca fascicularis; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D055495:Neurogenesis / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D000091042:Reelin Protein; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
D030161:Avian Proteins; D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; D060850:LIM-Homeodomain Proteins; C499523:Lhx1 protein, mouse; C489784:Lhx2 protein, mouse; C102995:Lhx5 protein, mouse; C117058:Lhx9 protein, mouse; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D014157:Transcription Factors; C000717128:Reln protein, mouse