title: Establishment and characterization of human bladder cancer cell lines BexBra1, BexBra2, and BexBra4.
abstract: Bladder cancer (BC) is the fourth most common cancer in the USA. In Brazil, BC represents 3% of the total existing carcinomas in the population and represents the second highest incidence among urological tumors. The majority of bladder cancer cell lines available were derived from Caucasians and established in the seventies or eighties. Thus, neoplasia development in these cells likely occurred in environment conditions vastly different than today. In the present study, we report the establishment and characterization of three Brazilian bladder cancer cell lines (BexBra1, BexBra2, and BexBra4). These cell lines may be helpful for dissecting the genetic and epigenetic aspects that trigger the progression of BC. Moreover, the development of a Brazilian representative of the disease will allow us to investigate the potential inter-racial differences of malignancy-associated phenotypes in bladder cancer.
authors.affiliation: Laboratory of Medical Investigation, Urology Department–LIM55, University of Sao Paulo Medical School, São Paulo, São Paulo, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Piantino
authors.forename: Juliana M
authors.initials: JM
authors.lastname: Sousa-Canavez
authors.forename: Victor
authors.initials: V
authors.lastname: Srougi
authors.forename: Fernanda
authors.initials: F
authors.lastname: Salvadori
authors.forename: Raphael
authors.initials: R
authors.lastname: Kato
authors.forename: Pedro Paulo R
authors.initials: PP
authors.lastname: Ayres
authors.forename: Miguel
authors.initials: M
authors.lastname: Srougi
authors.forename: Luiz Heraldo
authors.initials: LH
authors.lastname: Camara-Lopes
authors.forename: Gilka Jorge Figaro
authors.initials: GJ
authors.lastname: Gattás
authors.forename: Cintia
authors.initials: C
authors.lastname: Fridman
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Toledo
authors.forename: Isaque
authors.initials: I
authors.lastname: Santana
authors.forename: Kátia Ramos Moreira
authors.initials: KR
authors.lastname: Leite
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D015925:Cryopreservation; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051379:Mice; D016133:Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000473:pathology*