title: [Atrial myocytes KChIP2 mRNA expression in rheumatic heart disease patients with atrial fibrillation].
abstract: OBJECTIVE
  To detect the KChIP2 mRNA level in rheumatic heart disease patients with or without atrial fibrillation (AF) by real-time PCR.
  
  
  METHODS
  Right atrial appendage samples from rheumatic heart disease patients with (n = 17) or without AF (n = 13) were obtained during cardiac surgery. Total RNA was extracted from the atrial tissues, and the KChIP2 and Kv4.3 mRNA were detected by SYBR Green I real-time PCR with the GAPDH as the house keeping gene.
  
  
  RESULTS
  The ratio of KChIP2/GAPDH (0.1468 +/- 0.0452 vs. 0.2200 +/- 0.0388, P<0.01) and the ratio of Kv4.3/GAPDH (0.3946 +/- 0.1826 vs. 0.5257 +/- 0.1427, P<0.05) were significantly lower in AF patients compared to non-AF patients.
  
  
  CONCLUSIONS
  Down-regulated atrial KChIP2 and Kv4.3 mRNA expressions in rheumatic heart disease patients with chronic AF might be one of the molecular bases responsible for the down-regulation of the I(to) current density of AF.
authors.affiliation: Department of Myocardium Electrophysiology, Institute of Cardiovasology, Luzhou Medical College, Luzhou 646000, China.
authors.forename: Xiao-qiu
authors.initials: XQ
authors.lastname: Tan
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhi-fei
authors.initials: ZF
authors.lastname: Liu
authors.forename: Zhi-ru
authors.initials: ZR
authors.lastname: Bai
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Pei
authors.forename: Gui-lan
authors.initials: GL
authors.lastname: Chen
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Zeng
mesh_terms: D000328:Adult; D001281:Atrial Fibrillation / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D051605:Kv Channel-Interacting Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D012214:Rheumatic Heart Disease / Q000235:genetics; D051672:Shal Potassium Channels / Q000235:genetics
chemical_list: C496752:KCNIP2 protein, human; D051605:Kv Channel-Interacting Proteins; D012333:RNA, Messenger; D051672:Shal Potassium Channels