title: The histological development of acute antibody-mediated rejection in HLA antibody-incompatible renal transplantation.
abstract: BACKGROUND
  The aim of this study was to examine the development of acute antibody-mediated rejection in HLA antibody-incompatible renal transplantation in relation to the Banff 07 histological classification.
  
  
  METHODS
  Renal biopsies were scored using the Banff 07 diagnostic criteria, and paraffin-embedded sections were stained with the pan-leucocyte marker CD45.
  
  
  RESULTS
  Thirty-six patients had 72 renal biopsies. In biopsies performed 30 min after graft reperfusion, the mean number of CD45+ cells per glomerulus was higher than in control grafts (P < 0.04) and was associated with the donor-specific antibody (DSA) level at transplantation measured by microbeads (P < 0.01), and eight out of nine patients with greater than five CD45+ cells per glomerulus had early post-transplant rejection or oliguria, compared to 11 out of 20 with less than five cells per glomerulus (P < 0.01). In the first 10 days post-transplant, although peritubular capillary (PTC) leucocyte margination grade 3 and C4d deposition were specific for rejection, their sensitivities were low. PTC C4d staining was only seen in two out of 11 biopsies taken in the first 5 days after transplant, even in the presence of rejection, but was present in the majority of later biopsies with rejection. In biopsies stained for CD3, CD68 and CD20, it was notable that CD20+ cells were not seen during acute rejection, the infiltrates comprising CD3+ and CD68+ leucocytes.
  
  
  CONCLUSIONS
  Glomerular margination of leucocytes occurred early after transplantation and was associated with DSA level and early graft dysfunction. The Banff 07 PTC margination scoring system was easy to apply, especially when CD45 staining was used, and PTC margination grade 3 was always associated with clinical rejection.
authors.affiliation: Transplant Unit, University Hospitals Coventry and Warwickshire, Coventry, West Midlands, UK. Robert.Higgins@uhcw.nhs.uk
authors.forename: Rob
authors.initials: R
authors.lastname: Higgins
authors.forename: Daniel
authors.initials: D
authors.lastname: Zehnder
authors.forename: Klaus
authors.initials: K
authors.lastname: Chen
authors.forename: David
authors.initials: D
authors.lastname: Lowe
authors.forename: Joanna
authors.initials: J
authors.lastname: McKinnell
authors.forename: For T
authors.initials: FT
authors.lastname: Lam
authors.forename: Habib
authors.initials: H
authors.lastname: Kashi
authors.forename: Lam Chin
authors.initials: LC
authors.lastname: Tan
authors.forename: Chris
authors.initials: C
authors.lastname: Imray
authors.forename: Simon
authors.initials: S
authors.lastname: Fletcher
authors.forename: Nithya
authors.initials: N
authors.lastname: Krishnan
authors.forename: Rizwan
authors.initials: R
authors.lastname: Hamer
authors.forename: David
authors.initials: D
authors.lastname: Briggs
mesh_terms: D000293:Adolescent; D000328:Adult; D015935:Complement C4b / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007674:Kidney Diseases / Q000628:therapy; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology; D055815:Young Adult
chemical_list: D006680:HLA Antigens; D007074:Immunoglobulin G; D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d