title: [Production and characteristics of monoclonal antibodies to the diphtheria toxin].
abstract: Monoclonal antibodies to the diphtheria toxin were produced without cross reactivity with the thermolabile toxin (LT) from Escherichia coli; ricin; choleraic toxin; the SeA, SeB, SeE, SeI, and SeG toxins of staphylococcus; the lethal factor of the anthrax toxin; and the protective antigen of the anthrax toxin. A pair of antibodies for the quantitative determination of the diphtheria toxin in the sandwich variation of enzyme-linked immunosorbent assay (ELISA) was chosen. The determination limit of the toxin was 0.7 ng/ml in plate and 1.6 ng/ml in microchip ELISA. The presence of a secretion from the nasopharynx lavage did not decrease the sensitivity of the toxin determination by sandwich ELISA. The immunization of mice with the diphtheria toxin and with a conjugate of the diphtheria toxin with polystyrene microspheres demonstrated that the conjugate immunization resulted in the formation of hybridoma clones which produced antibodies only to the epitopes of the A fragment of the diphtheria toxin. The immunization with the native toxin caused the production of hybridoma clones which predominantly produced antibodies to the epitopes of the B fragment.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. valyakina@ibch.ru
authors.forename: T I
authors.initials: TI
authors.lastname: Valiakina
authors.forename: O E
authors.initials: OE
authors.lastname: Lakhtina
authors.forename: R L
authors.initials: RL
authors.lastname: Komaleva
authors.forename: M A
authors.initials: MA
authors.lastname: Simonova
authors.forename: L V
authors.initials: LV
authors.lastname: Samokhvalova
authors.forename: N S
authors.initials: NS
authors.lastname: Shoshina
authors.forename: N A
authors.initials: NA
authors.lastname: Kalinina
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Rubina
authors.forename: M A
authors.initials: MA
authors.lastname: Filippova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Vertiev
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology; D004167:Diphtheria Toxin / Q000032:analysis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D004167:Diphtheria Toxin; D000939:Epitopes