title: European genotype of porcine reproductive and respiratory syndrome (PRRSV) infects monocyte-derived dendritic cells but does not induce Treg cells.
abstract: The aim of this study was to characterize the immune responses of DCs after infection with four different EU strains of PRRSV and whether they show any ability to immunomodulate T cells activation. Our results show that all EU strains can efficiently infect and replicate in DCs. Nevertheless, SLA-II levels remained unaltered in DC infected by all EU PRRSV strains, whereas SLA-I expression was only reduced when strain 2992 was used. IL-10 production was induced by three EU PRRSV strains, being strain 2992 the highest inducer. However, no induction of Treg cells, measured by CD25 and Foxp3 expression on lymphocytes co-cultured with infected DCs, was found. TGF-beta induction was not detected in DC infected with any EU strain tested. In conclusion, DCs infected with EU PRRSV strains exhibited an unbalanced ability to stimulate T cell response and was strain dependent. However, Treg cells were not induced, at least in vitro.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, Mexico.
authors.forename: Erika
authors.initials: E
authors.lastname: Silva-Campa
authors.forename: Lorena
authors.initials: L
authors.lastname: Cordoba
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fraile
authors.forename: Lilian
authors.initials: L
authors.lastname: Flores-Mendoza
authors.forename: María
authors.initials: M
authors.lastname: Montoya
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000821:virology*; D005060:Europe; D051858:Forkhead Transcription Factors / Q000276:immunology; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000276:immunology; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II; D053645:Interleukin-2 Receptor alpha Subunit; D008213:Lymphocyte Activation / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000503:physiopathology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D013552:Swine / Q000276:immunology / Q000821:virology; D050378:T-Lymphocytes, Regulatory / Q000821:virology*; D014779:Virus Replication / Q000502:physiology
chemical_list: D016207:Cytokines; D051858:Forkhead Transcription Factors; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D053645:Interleukin-2 Receptor alpha Subunit; C438668:swine leukocyte antigen