Construction and development of a new single-column simulated moving bed system on the laboratory scale.
Simulated moving bed (SMB) chromatography combines high productivity and high purities with reduced buffer consumption. We have developed a laboratory scale single column SMB (SC-SMB) unit with all four separation zones in one column. Distributors embedded within the chromatographic medium allow introduction and withdrawal of liquid between the zones. This single column unit exhibits homogenous packing in all zones, reduced headspace, less complex tubing, fewer valves, and almost undisturbed plug flow between the separation zones. The separation performance of the column was investigated with two different binary model mixtures. Furthermore, the SC-SMB unit is operated with a modified AKTA Explorer workstation, which has been specifically developed for the handling of biological fluids.
Buhlert
Kerstin
K
Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria.
Lehr
Markus
M
Jungbauer
Alois
A
D002845:Chromatography / Q000295:instrumentation*; D019411:Clinical Laboratory Techniques / Q000295:instrumentation*; D045304:Cytochromes c / Q000302:isolation & purification; D003911:Dextrans / Q000737:chemistry; D009113:Muramidase / Q000302:isolation & purification; D015203:Reproducibility of Results; D014805:Vitamin B 12 / Q000302:isolation & purification
D003911:Dextrans; D045304:Cytochromes c; D009113:Muramidase; D014805:Vitamin B 12