Detection of IFN-alpha produced in the presence of plasma gamma-globulin fraction proteins.
Interferon-alpha was detected in IFN pool produced by human leukocytes in the presence of gamma-globulin fraction proteins, copper and zinc cations, and metal-modified gamma-globulins. The cytokine appeared in culture medium at early terms (24 h) of incubation, is characterized by acid resistance, and is neutralized by antibodies to IFN-alpha. The content of IFN-alpha in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum gamma-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation.
Cheknyov
S B
SB
Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. cheknev@riem.ru
Babayants
A A
AA
Efremova
I E
IE
Yushkovets
E N
EN
D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D016898:Interferon-alpha / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D012712:Serum Globulins / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry; D005719:gamma-Globulins / Q000737:chemistry / Q000494:pharmacology*
D016898:Interferon-alpha; D012709:Serum Albumin; D012712:Serum Globulins; D005719:gamma-Globulins; C008930:plasma protein fraction; D003300:Copper; D015032:Zinc; D000075462:Serum Albumin, Human