Water soluble polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.
A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a water-soluble polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in polymer films. The degradation of the polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
Zhang
Qiao
Q
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Cheng
Si-Xue
SX
Zhang
Xian-Zheng
XZ
Zhuo
Ren-Xi
RX
D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012995:Solubility; D014162:Transfection; D014867:Water
D008055:Lipids; C086724:Lipofectamine; D010455:Peptides; C008965:alpha,beta-poly((2-hydroxyethyl)-aspartamide); D014867:Water; D004247:DNA