title: Characterization of the CtsR stress response regulon in Lactobacillus plantarum.
abstract: Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Delta ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR-clpC operon and hsp1.
authors.affiliation: Department of Biomedical Sciences, University of Foggia, Viale L. Pinto, 71100 Foggia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fiocco
authors.forename: Vittorio
authors.initials: V
authors.lastname: Capozzi
authors.forename: Michael
authors.initials: M
authors.lastname: Collins
authors.forename: Anna
authors.initials: A
authors.lastname: Gallone
authors.forename: Pascal
authors.initials: P
authors.lastname: Hols
authors.forename: Jean
authors.initials: J
authors.lastname: Guzzo
authors.forename: Stephanie
authors.initials: S
authors.lastname: Weidmann
authors.forename: Aurélie
authors.initials: A
authors.lastname: Rieu
authors.forename: Tarek
authors.initials: T
authors.lastname: Msadek
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spano
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D006360:Heat-Shock Proteins; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D018085:Regulon / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; C118338:CtsR protein, bacteria; D006360:Heat-Shock Proteins; D012097:Repressor Proteins