1H NMR-based protocol for the detection of adulterations of refined olive oil with refined hazelnut oil.
A (1)H NMR analytical protocol for the detection of refined hazelnut oils in admixtures with refined olive oils is reported according to ISO format. The main purpose of this research activity is to suggest a novel analytical methodology easily usable by operators with a basic knowledge of NMR spectroscopy. The protocol, developed on 92 oil samples of different origins within the European MEDEO project, is based on (1)H NMR measurements combined with a suitable statistical analysis. It was developed using a 600 MHz instrument and was tested by two independent laboratories on 600 MHz spectrometers, allowing detection down to 10% adulteration of olive oils with refined hazelnut oils. Finally, the potential and limitations of the protocol applied on spectrometers operating at different magnetic fields, that is, at the proton frequencies of 500 and 400 MHz, were investigated.
Mannina
Luisa
L
Department of S.T.A.A.M., University of Molise, I-86100 Campobasso, Italy. mannina@unimol.it
D'Imperio
Marco
M
Capitani
Donatella
D
Rezzi
Serge
S
Guillou
Claude
C
Mavromoustakos
Thomas
T
Vilchez
María Dolores Molero
MD
Fernández
Antonio Herrera
AH
Thomas
Freddy
F
Aparicio
Ramon
R
D031211:Corylus / Q000737:chemistry*; D004042:Dietary Fats, Unsaturated / Q000032:analysis; D005506:Food Contamination; D005524:Food Technology / Q000379:methods* / Q000592:standards; D005638:Fruit / Q000737:chemistry; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015233:Models, Statistical; D009754:Nuts / Q000737:chemistry; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results
D004042:Dietary Fats, Unsaturated; D000069463:Olive Oil; D010938:Plant Oils