Multiplex chemiluminescent immunoassay based on silica colloidal crystal beads.
The paper presented a novel multiplex chemiluminescence (CL) immunoassay method, which was based on silica colloidal crystals beads (SCCBs) as carriers using enhanced chemiluminescence (CL) detection of a horseradish peroxidase (HRP) catalyzing the luminal reaction. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. The experimental conditions were optimized and analytical performance was studied. Results showed that the SCCBs as supports were much sensitive (0.05 ng/ml IgG) than the glass beads (18 ng/ml IgG) and the planar carriers (125 ng/ml IgG). A multiplex immunoassay suggested the feasibility of high throughput applications. This novel immunoassay system provided a simple, sensitive and low-cost approach for multianalyte immunoassay without using of expensive array detector.
Pei
Xiaoping
X
School of Clinical Medicine, Department of Hematology, Affiliated Zhongda Hospital, Southeast University, Nanjing 210009, China.
Zhao
Yuanjin
Y
Zhao
Xiangwei
X
Xu
Hua
H
Chen
Baoan
B
Gu
Zhongze
Z
D015374:Biosensing Techniques / Q000295:instrumentation*; D003102:Colloids / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
D003102:Colloids; D012822:Silicon Dioxide