title: Evaluation of a competitive ELISA for antibody detection against avian influenza virus.
abstract: Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or following vaccination of the AIV H9 or H5 subtypes in chickens, ducks and geese. Next, we tested the sensitivity and specificity of the cELISA with sera from twelve different animal species, and compared these results with those obtained by the hemagglutination-inhibition (HI) test, the "gold standard" in AIV sera surveillance, a second commercially-available cELISA (IZS ELISA), or the agar gel precipitation (AGP) test. Compared with the HI test, the sensitivities and specificities of cELISA were 95% and 96% in chicken, 86% and 88% in duck, 97% and 100% in turkey, 100% and 87% in goose, and 91% and 97% in swine, respectively. The sensitivities and specificities of the cELISA in this study were higher than those of IZS ELISA for the duck, turkey, goose, and grey partridge sera samples. The results of AGP test against duck and turkey sera also showed significant correlation with the results of cELISA (R-value >0.9). In terms of flock sensitivity, the cELISA correlated better with the HI test than with commercially-available indirect ELISAs, with 100% flock sensitivity.
authors.affiliation: Green Cross Veterinary Products, Youngin 446-569, Korea.
authors.forename: Dae Sub
authors.initials: DS
authors.lastname: Song
authors.forename: Youn Jeong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Ok Mi
authors.initials: OM
authors.lastname: Jeong
authors.forename: Yong Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Park
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Yoo
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Jeon
authors.forename: Jun Hun
authors.initials: JH
authors.lastname: Kwon
authors.forename: Gun Woo
authors.initials: GW
authors.lastname: Ha
authors.forename: Bo Kyu
authors.initials: BK
authors.lastname: Kang
authors.forename: Chul Seung
authors.initials: CS
authors.lastname: Lee
authors.forename: Hye Kwon
authors.initials: HK
authors.lastname: Kim
authors.forename: Byeong Yeal
authors.initials: BY
authors.lastname: Jung
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Kim
authors.forename: Jin Sik
authors.initials: JS
authors.lastname: Oh
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001717:Birds; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006736:Horses; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology; D005585:Influenza in Birds / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013045:Species Specificity; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines