title: Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry.
abstract: A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic tool for avian influenza virus detection.
authors.affiliation: Institute of Mechanics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Qi
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Tian
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Yan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cao
authors.forename: Ke-Gong
authors.initials: KG
authors.lastname: Tian
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000302:isolation & purification*; D020527:Microscopy, Scanning Probe / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014771:Virion / Q000648:ultrastructure*