title: Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay.
abstract: We evaluated a multiplexed bead-based assay (xMAP Pneumococcal Immunity assay from Luminex) for the simultaneous determination of antibodies against 14 capsular polysaccharides. Post-vaccination (Pneumovax) antibody concentrations were measured in 35 healthy children, 40 healthy adults, 99 consecutive patients with increased susceptibility to respiratory infection, and 24 patients with a deficient anti-polysaccharide antibody response. The serotype-specific lower 5th percentile (cutoff) value for the post-immunization antibody concentration was determined in healthy individuals. Eleven of 99 patients (11%) failed to mount a response that was >5th percentile of controls for at least 6 of the 14 serotypes tested, whereas only 3 of 75 controls (4%) failed to do so. All patients with known anti-polysaccharide antibody deficiency failed to mount a response that was >5th percentile of controls for at least 6 of the 14 serotypes tested. The XMAP pneumococcal immunity panel appears useful for identifying individuals with a low response to the unconjugated pneumococcal vaccine.
authors.affiliation: Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Biomedical Sciences Group, Catholic University Leuven, Leuven, Belgium.
authors.forename: Heleen
authors.initials: H
authors.lastname: Borgers
authors.forename: Leen
authors.initials: L
authors.lastname: Moens
authors.forename: Capucine
authors.initials: C
authors.lastname: Picard
authors.forename: Axel
authors.initials: A
authors.lastname: Jeurissen
authors.forename: Marc
authors.initials: M
authors.lastname: Raes
authors.forename: Kate
authors.initials: K
authors.lastname: Sauer
authors.forename: Marijke
authors.initials: M
authors.lastname: Proesmans
authors.forename: Kris
authors.initials: K
authors.lastname: De Boeck
authors.forename: Jean-Laurent
authors.initials: JL
authors.lastname: Casanova
authors.forename: Isabelle
authors.initials: I
authors.lastname: Meyts
authors.forename: Xavier
authors.initials: X
authors.lastname: Bossuyt
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000276:immunology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D022242:Pneumococcal Vaccines