Study on expression of laminin in patients with intractable epilepsy.
In this study, we examined differences in serum laminin expression in patients with intractable epilepsy. Our results suggest that elevated laminin may contribute to the pathogenesis of intractable epilepsy. ELISA and western blots were used to measure laminin in the serum of 30 intractable epilepsy patients, 46 nonintractable epilepsy patients, and 20 normal subjects. By ELISA, serum laminin levels were greater in intractable epilepsy patients (177.396 +/- 30.602) and nonintractable epilepsy patients (121.915 +/- 35.215) than in normal control subjects (67.474 +/- 7.197); laminin was significantly greater in the intractable epilepsy group than in the nonintractable epilepsy group. In western blots, the optical density ratio of laminin to ss-actin was 0.871 +/- 0.032 for the intractable epilepsy group, 0.686 +/- 0.017 for the nonintractable epilepsy group, and 0.385 +/- 0.024 for the normal control group. The optical density ratios of the intractable and nonintractable epilepsy groups were higher than those for the normal control group, and the intractable epilepsy group was even greater than the nonintractable epilepsy group. Thus, laminin is significantly increased in epilepsy patients, and this increase is more profound in intractable epilepsy patients.
Wu
Yuan
Y
Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. wuyuan90@126.com
Feng
Yun
Y
Pang
Jia-Rong
JR
Tang
Mei
M
Liu
Xiu-Ying
XY
Li
Jia-Quan
JQ
Wang
Xue-Feng
XF
D000199:Actins / Q000097:blood; D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004827:Epilepsy / Q000097:blood*; D005260:Female; D006801:Humans; D007797:Laminin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000199:Actins; C478221:LAMB1 protein, human; D007797:Laminin