title: Histamine stimulates interleukin-6 production through histamine H1 receptors in human amnion cells.
abstract: OBJECTIVE
  Previous studies have stated that maternal allergic diseases are associated with increased risk of preterm labor/delivery, but the underlying mechanisms remain unclear. This study tested the hypothesis that histamine induces interleukin (IL)-6 production in amnion cells.
  
  
  METHODS
  Using cultured human amnion cells, we examined expression of histamine receptors and effects of histamine on IL-6 production.
  
  
  RESULTS
  Reverse transcription-polymerase chain reaction and Western blotting revealed expression of histamine H1 receptor (H1R) and H2 receptor (H2R) in human amnion. Histamine stimulation significantly increased concentrations of IL-6 in conditioned medium, as did tumor necrosis factor-alpha and IL-1beta in positive controls. In addition, the H1R antagonist olopatadine significantly blocked histamine-induced production of IL-6, whereas the H2R antagonist ranitidine did not.
  
  
  CONCLUSIONS
  Histamine appears to induce IL-6 production through H1R in human amnion cells.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Morimitsu
authors.forename: Seiji
authors.initials: S
authors.lastname: Sumigama
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kotani
authors.forename: Yukio
authors.initials: Y
authors.lastname: Mano
authors.forename: Kanako
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masako
authors.initials: M
authors.lastname: Araki-Taguchi
authors.forename: Eiko
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hayakawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Okada
authors.forename: Atsuo
authors.initials: A
authors.lastname: Itakura
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kikkawa
mesh_terms: D000650:Amnion / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015153:Blotting, Western; D003990:Dibenzoxepins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006632:Histamine / Q000276:immunology / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000276:immunology; D000069605:Olopatadine Hydrochloride; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011969:Receptors, Histamine H1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011970:Receptors, Histamine H2 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D003990:Dibenzoxepins; D006634:Histamine H1 Antagonists; D015850:Interleukin-6; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D000069605:Olopatadine Hydrochloride; D006632:Histamine