Effect of particulate antigenic stimulation or in vivo administration of interleukin-6 on the level of steroidogenic enzymes in adrenal glands and lymphoid tissues of mice with parallel alteration in endogenous inflammatory cytokine level.
To study the effects of sheep red blood cells (SRBC), viable Escherichia coli inoculation and IL-6 administration on steroidogenesis, activities and expression of hydroxy steroid dehydrogenase enzymes (3betaHSD and 17betaHSD) were measured in lymphoid organs of control and infected mice after 3 weeks treatment. Serum testosterone and cytokine levels were also estimated. Reduced expression of 3betaHSD4 was found in the spleen of treated groups as compared to control, whereas the 3betaHSD4 expression was increased in the thymus and lymph gland after stimulation. Reduced serum testosterone level was observed after antigenic stimulation and also altered serum IL-12p70, TNF-alpha, IFN-gamma, MCP-1 or IL-10 level. These studies provide the evidence of expression of 3betaHSD4 enzyme in the murine lymphoid organs after particulate antigen stimulation along with a parallel alteration in endogenous cytokine level.
Mukhopadhyay
Rupanjan
R
Department of Physiology, Immunology Laboratory, University Colleges of Science and Technology, University of Calcutta, West Bengal, Kolkata, India.
Mishra
Manoj K
MK
Basu
Anirban
A
Bishayi
Biswadev
B
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015850:Interleukin-6 / Q000494:pharmacology*; D008221:Lymphoid Tissue / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D008297:Male; D051379:Mice; D012756:Sheep; D013739:Testosterone / Q000097:blood
D000942:Antigens, Bacterial; D016207:Cytokines; D015850:Interleukin-6; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases