Typing of Candida isolates from patients with invasive infection and concomitant colonization.
We investigated the relationship between colonizing and invasive isolates from patients with candidaemia. Molecular typing was performed using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST). We found MLST to be sufficient for typing Candida isolates, and that surveillance cultures are helpful in predicting concomitant invasive isolates, but not necessarily the pathogen involved in subsequent episodes.
Brillowska-Dabrowska
Anna
A
Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark. abrilld@yahoo.com
Bergmann
Olav
O
Jensen
Irene Moeller
IM
Jarløv
Jens Otto
JO
Arendrup
Maiken C
MC
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D002353:Carrier State / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D016469:Fungemia / Q000382:microbiology*; D006801:Humans; D016533:Mycological Typing Techniques; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal