title: Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA.
abstract: A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Yun
authors.forename: Z
authors.initials: Z
authors.lastname: Ni
authors.forename: G Q
authors.initials: GQ
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: J G
authors.initials: JG
authors.lastname: Huang
authors.forename: Y M
authors.initials: YM
authors.lastname: Zhang
authors.forename: J P
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D004372:Ducks; D004371:Hepatitis Virus, Duck / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*; D016019:Survival Analysis; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral