Synthesis and thermally reversible assembly of DNA-gold nanoparticle cluster conjugates.
We describe the facile synthesis of stable gold nanoparticle clusters densely functionalized with DNA (DNA-AuNP clusters) using dithiothreitol and monothiol DNA and their thermally reversible assembly properties. The size of the clusters exhibits a very narrow distribution and can be easily controlled by adjusting the stoichiometry of dithiothreitol and DNA, leading to a variety of colors due to the surface plasmon resonance of the AuNP clusters. Importantly, the DNA-AuNP clusters exhibit highly cooperative melting properties with distinctive and diverse color changes depending on their size. The selective and sensitive colorimetric detection of target sequences was demonstrated based upon the unique properties of the DNA-AuNP clusters.
Kim
Ji-Young
JY
Department of Materials Science and Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea.
Lee
Jae-Seung
JS
D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties; D013696:Temperature
D046911:Macromolecular Substances; D006046:Gold; D004247:DNA