Up-regulation of ferritin ubiquitination in skeletal muscle of transgenic rats bearing the G93A hmSOD1 gene mutation.
In the present study we measured the levels of protein carbolnyls and the H and L subunits of ferritin in three hind limb muscles, [Extensor digitorum longus, Tibialis anterior and Soleus] of transgenic rats bearing the G93A hmSOD1 gene and of their non-transgenic littermates. All of the muscles from the transgenic animals showed significantly higher protein carbonyl levels, compared to the respective muscles from control non-transgenic animals. In two muscles (Tibialis anterior and Soleus) from transgenic rats, both L and H subunits of ferritin were upregulated. Moreover, we observed that the electrophoretic mobility of both ferritin subunits was retarded which indicates their post-translational modification. Ferritin immunoprecipitation experiments show an increased ubiquitination of both H and L ferritin in all muscles from the transgenic animals. Our data show for the first time that ferritin ubiquitination could be responsible for oxidative stress in muscles of rats bearing the G93A hmSOD1, consequently ferritin is not able to control the labile iron pool.
Halon
Malgorzata
M
Department of Bioenergetics and Physiology of Exercise, Medical University of Gdansk, Poland.
Sielicka-Dudzin
Alicja
A
Wozniak
Michal
M
Ziolkowski
Wiesław
W
Nyka
Walenty
W
Herbik
Magdalena
M
Grieb
Pawel
P
Figarski
Adam
A
Antosiewicz
Jedrzej
J
D000818:Animals; D003124:Colorimetry; D004586:Electrophoresis; D005293:Ferritins / Q000378:metabolism*; D006614:Hindlimb / Q000378:metabolism; D047468:Immunoprecipitation; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism*; D009929:Organ Size / Q000235:genetics; D050050:Protein Carbonylation / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D054875:Ubiquitination / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
D005293:Ferritins; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1