A second gene encodes the anti-diuretic hormone in the insect, Rhodnius prolixus.
In the haematophagous insect, Rhodnius prolixus, a rapid diuresis following engorgement of vertebrate blood is under the control of two main diuretic hormones: a corticotropin-releasing factor (CRF)-related peptide andserotonin (5HT). A CAP2b (CAPA)-related peptide is involved in the termination of this diuresis, and we have recently identified a gene, now referred to as RhoprCAPA-alpha, encoding CAPA peptides in R. prolixus. Here we identify a second gene, RhoprCAPA-beta, which also encodes CAPA peptides and characterize its expression in fifth-instar and adults. The RhoprCAPA-beta gene is more highly expressed in the CNS than the RhoprCAPA-alpha gene, but neither gene is expressed in other tested adult tissues. Both genes are expressed in a subset of immunoreactive neurons identified using an antisera which recognizes CAP2b-related peptides. The expression of each paralog is modified by feeding and we propose this to be a result of requirements of anti-diuretic regulation during salt and water homeostasis.
Paluzzi
Jean-Paul
JP
Department of Biology, University of Toronto Mississauga, Mississauga, Ontario, Canada L5L 1C6. jeanpaul.paluzzi@utoronto.ca
Orchard
Ian
I
D000375:Aging / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism; D005247:Feeding Behavior; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D016678:Genome / Q000235:genetics; D007150:Immunohistochemistry; D007814:Larva / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics*; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012239:Rhodnius / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D009479:Neuropeptides; D009842:Oligopeptides; D012333:RNA, Messenger; C095817:cardioacceleratory peptide 2b; D011761:Pyrrolidonecarboxylic Acid