title: Lipids from attenuated and virulent Babesia bovis strains induce differential TLR2-mediated macrophage activation.
abstract: Babesia bovis is an intraerythrocytic apicomplexan protozoa of cattle that causes an acute infection with parasite persistence. Babesiosis limitation depends on macrophages, essential effector cells of the host innate defense, which generate inflammatory cytokines and nitric oxide. Herein, we report quantitative differences in the lipid composition of merozoites from two B. bovis strains with polar behaviour: attenuated R1A and virulent S2P. Accordingly, we observed a distinct inflammatory response induced by the total lipids of R1A (L(A)) and S2P (L(V)) in murine peritoneal macrophages. L(A) and particularly its fractions phosphatidic acid and phosphatidylserine+phosphatidylinositol (PS+PI), produced a strong activation of these cells with lipid body formation, cyclooxygenase-2 expression and pro-inflammatory TNFalpha, IL-6 and KC secretion. Although L(V) did not activate these cells, the corresponding PS+PI fraction induced TNFalpha, IL-6 and KC release. Therefore, these facts might be suggesting the presence of an inhibitor in L(V). Furthermore, the employment of wild type and toll like receptor 2 knockout (TLR2KO) mice allowed us to demonstrate that macrophage activation by the stimulating lipid fractions was mediated through TLR2. Interestingly, only L(A) activated the extracellular signal-regulated kinases 1 and 2 (ERK1/2). Inhibitory studies employing UO126, indicated that the ERK pathway was required for TNFalpha, IL-6 and KC release. In conclusion, the absence of inflammatory response observed with the lipids of S2P virulent strain could constitute an evasion mechanism of the innate immune response enabling parasite establishment in the host.
authors.affiliation: Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. paradife@fmed.uba.ar
authors.forename: G
authors.initials: G
authors.lastname: Gimenez
authors.forename: K G
authors.initials: KG
authors.lastname: Magalhães
authors.forename: M L
authors.initials: ML
authors.lastname: Belaunzarán
authors.forename: C V
authors.initials: CV
authors.lastname: Poncini
authors.forename: E M
authors.initials: EM
authors.lastname: Lammel
authors.forename: S M
authors.initials: SM
authors.lastname: Gonzalez Cappa
authors.forename: P T
authors.initials: PT
authors.lastname: Bozza
authors.forename: E L D
authors.initials: EL
authors.lastname: Isola
mesh_terms: D000818:Animals; D016793:Babesia bovis / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000469:parasitology; D052940:Merozoites / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D014774:Virulence / Q000187:drug effects
chemical_list: D016207:Cytokines; D008055:Lipids; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; D015232:Dinoprostone