Probing the relevance of lectin clustering for the reliable evaluation of multivalent carbohydrate recognition.
Experiments by Surface Plasmon Resonance (SPR) illustrate the relevance of lectin density for the reliable evaluation of binding efficiencies in surface-based multivalent carbohydrate recognition. The difference between affinity data obtained by solution and surface-based experiments is also stressed.
Munoz
Eva Maria
EM
Departamento de Química Orgánica, Facultad de Química, and Unidad de RMN de Biomoléculas Asociada al CSIC, Universidad de Santiago de Compostela, Avda. de las Ciencias S.N. 15782 Santiago de Compostela, Spain.
Correa
Juan
J
Fernandez-Megia
Eduardo
E
Riguera
Ricardo
R
D003208:Concanavalin A / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D008358:Mannose / Q000737:chemistry*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties
D050091:Dendrimers; D003208:Concanavalin A; C028914:triethylene glycol; D011092:Polyethylene Glycols; D005707:Gallic Acid; D008358:Mannose