title: [Immunochromatographic and latex-agglutination systems for diphtheria toxin detection].
abstract: The use of two monoclonal antibody types specific to different epitopes of diphtheria toxin systems have been developed to reveal diphtheria corynebacteria toxigenicity rapidly based on immunochromatographic and latex-agglutination detection of the diphtheria toxin. The methods have been tested on a sample of 36 clinical isolates. The possibility of significant detection of the toxigenic properties of the Corynebacterium strain, grown for 1 day, has been demonstrated. The developed methods allow for the detection of diphtheria toxin in concentrations of 3-4 ng/ml. The developed test systems are a perspective tool for diphtheria diagnostics because of significant time shortening as compared to traditional microbiological methods.
authors.forename: N A
authors.initials: NA
authors.lastname: Byzova
authors.forename: V V
authors.initials: VV
authors.lastname: Sviridov
authors.forename: N F
authors.initials: NF
authors.lastname: Gavrilova
authors.forename: E N
authors.initials: EN
authors.lastname: Raspopova
authors.forename: I V
authors.initials: IV
authors.lastname: Iakovleva
authors.forename: A N
authors.initials: AN
authors.lastname: Generalova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Lukin
authors.forename: V V
authors.initials: VV
authors.lastname: Cherkasova
authors.forename: A V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: B B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002845:Chromatography / Q000379:methods; D003353:Corynebacterium diphtheriae / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D004165:Diphtheria / Q000382:microbiology; D004167:Diphtheria Toxin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin