title: Effects of angiopoietin-1 on attachment and metastasis of human gastric cancer cell line BGC-823.
abstract: OBJECTIVE
  To evaluate the effects of angiopoietin-1 (Ang-1) on adhesion of gastric cancer cell line BGC-823 and expression of integrin beta1, CD44V6, urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-2 (MMP-2).
  
  
  METHODS
  BGC-823 cells were transfected transiently with adenovirus-Ang-1 (Ad-Ang-1). Cells transfected transiently with adenovirus-green fluorescent protein (Ad-GFP) and untransfected cells were used as a negative and blank control group, respectively. The cell adhesion rate between cell and extracellular matrix (ECM) was determined by cell adhesion assay. To investigate whether Ang-1 could reinforce gastric carcinoma metastasis, we performed migration and invasion assays in BGC-823 cells. The mRNA and protein expression of integrin beta1, CD44V6, uPA and MMP-2 were detected by reverse transcription polymerase chain reaction and Western blotting, respectively. The expression of integrin beta1 and CD44V6 was measured by immunohistochemistry.
  
  
  RESULTS
  BGC-823 cells were transfected successfully. The adhesion rate increased significantly in the Ad-Ang-1 group (P < 0.05). The Ad-Ang-1-transfected group had a significant increase in migration and invasion compared with that of the mock-transfected and Ad-GFP groups. The mRNA and protein expression of integrin beta1, CD44V6, uPA and MMP-2 in the Ad-Ang-1 group was higher than that in the Ad-GFP and blank control groups (P < 0.05). Compared with mock-transfected and Ad-GFP groups, integrin beta1 and CD44V6 expression intensity greatly increased (P < 0.05).
  
  
  CONCLUSIONS
  Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase expression of integrin beta1 and CD44V6, by which cell adhesion and metastasis to the ECM are promoted.
authors.affiliation: Department of Gastroenterology, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China. ouxilong@126.com
authors.forename: Xi-Long
authors.initials: XL
authors.lastname: Ou
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Chen
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Sun
authors.forename: Cheng
authors.initials: C
authors.lastname: Hang
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Yun-Yan
authors.initials: YY
authors.lastname: Guan
authors.forename: Fang
authors.initials: F
authors.lastname: Yan
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D042683:Angiopoietin-1 / Q000096:biosynthesis* / Q000502:physiology; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D019012:Integrin beta1 / Q000096:biosynthesis; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
chemical_list: D042683:Angiopoietin-1; C112697:CD44v6 antigen; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D014568:Urokinase-Type Plasminogen Activator; D020778:Matrix Metalloproteinase 2