Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant.
Cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. Reliable results cannot be obtained, however, when cultures contain contamination. This report discusses the observation and identification of mobile black specks observed in multiple cell lines. Cultures of the contamination were grown, and DNA was purified from isolated colonies. The 16S rDNA gene was PCR amplified using primers that will amplify the gene from many genera, and then sequenced. Sequencing results matched the members of the genus Achromobacter, bacteria common in the environment. Achromobacter species have been shown to be resistant to multiple antibiotics. Attempts to decontaminate the eukaryotic cell culture used multiple antibiotics at different concentrations. The contaminating Achromobacter was eventually eliminated, without permanently harming the eukaryotic cells, using a combination of the antibiotics ciprofloxacin and piperacillin.
Gray
Jennifer Sue
JS
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
Birmingham
Janette Marie
JM
Fenton
Jenifer Imig
JI
D041721:3T3-L1 Cells; D042402:Achromobacter / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D018929:Cell Culture Techniques / Q000592:standards; D002478:Cells, Cultured; D003116:Color; D003470:Culture Media / Q000032:analysis / Q000592:standards; D004340:Drug Contamination; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D044967:Serum / Q000382:microbiology; D020298:U937 Cells
D000900:Anti-Bacterial Agents; D003470:Culture Media