title: Effects of gadolinium chloride and glycine on hepatic and pancreatic tissue damage in alcoholic pancreatitis.
abstract: OBJECTIVE
  Systemic complications in alcoholic pancreatitis are supposed to be aggravated by inflammatory liver damage. Resident macrophages including hepatic Kupffer cells play a pivotal role in mediating systemic complications in severe necrotizing pancreatitis (SNP). The aim of this study was to evaluate the effects of Kupffer cell inhibition on the inflammatory liver damage in experimental alcoholic pancreatitis.
  
  
  METHODS
  Rats were fed with either alcohol or control diet for 6 weeks before induction of SNP. Animals were allocated into 4 groups: healthy controls, controls with SNP, SNP with gadolinium chloride or glycine (permanent vs temporary inhibition of hepatic Kupffer cells) prophylaxis. Hepatic microcirculation and morphologic damage of the liver and pancreas were assessed.
  
  
  RESULTS
  Alcohol feeding and SNP increased hepatic and pancreatic injury compared with SNP alone. Gadolinium chloride and glycine improved hepatic microcirculation. In contrast, pancreatic and hepatic morphological damage was reduced by gadolinium chloride but not by glycine.
  
  
  CONCLUSIONS
  Alcohol exposure aggravates hepatic and pancreatic injury in SNP. Gadolinium chloride reduces both microcirculatory and morphological damage, whereas glycine did not improve histological damage.
authors.affiliation: Department of General Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Schneider
authors.forename: Thilo
authors.initials: T
authors.lastname: Hackert
authors.forename: Thomas
authors.initials: T
authors.lastname: Longerich
authors.forename: Werner
authors.initials: W
authors.lastname: Hartwig
authors.forename: Stefan
authors.initials: S
authors.lastname: Fritz
authors.forename: Ralf
authors.initials: R
authors.lastname: Krych
authors.forename: Franco
authors.initials: F
authors.lastname: Fortunato
authors.forename: Martha-Maria
authors.initials: MM
authors.lastname: Gebhard
authors.forename: Jens
authors.initials: J
authors.lastname: Werner
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000431:Ethanol; D005682:Gadolinium / Q000494:pharmacology*; D005998:Glycine / Q000494:pharmacology*; D018684:Glycine Agents / Q000494:pharmacology; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D008102:Liver Circulation / Q000187:drug effects; D008107:Liver Diseases / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000209:etiology / Q000517:prevention & control*; D019512:Pancreatitis, Alcoholic / Q000139:chemically induced / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018684:Glycine Agents; D000431:Ethanol; D005682:Gadolinium; C038958:gadolinium chloride; D005998:Glycine