The ToxT-dependent methyl-accepting chemoreceptors AcfB and TcpI contribute to Vibrio cholerae intestinal colonization.
Vibrio cholerae colonizes the human intestine and causes the acute diarrheal disease cholera. Flagellar-mediated chemotaxis contributes to intestinal colonization as well as infectivity. The virulence-regulatory protein ToxT activates transcription of the genes encoding the major virulence factors cholera toxin and toxin coregulated pilus. ToxT additionally activates transcription of two genes, tcpI and acfB, located within the Vibrio Pathogenicity Island predicted to encode methyl-accepting chemoreceptors. We show that disruption of either tcpI or acfB individually does not noticeably affect V. cholerae intestinal colonization within the infant mouse, but disruption of both tcpI and acfB leads to a decrease in intestinal colonization. These results suggest that TcpI and AcfB may have overlapping or redundant chemotactic functions that contribute to V. cholerae intestinal colonization.
Chaparro
Adriana Paola
AP
South Texas Center for Emerging Infectious Diseases and Department of Biology, University of Texas San Antonio, San Antonio, TX, USA.
Ali
Syed Khalid
SK
Klose
Karl E
KE
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D015169:Colony Count, Microbial; D041981:Gastrointestinal Tract / Q000382:microbiology*; D017353:Gene Deletion; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D014734:Vibrio cholerae / Q000254:growth & development / Q000472:pathogenicity*; D014774:Virulence
C088551:AcfB protein, Vibrio cholerae; D001426:Bacterial Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; C097245:TcpI protein, Vibrio cholerae; D014157:Transcription Factors; C075626:tcpN protein, Vibrio cholerae