title: High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity.
abstract: Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
authors.affiliation: UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, Araraquara, São Paulo, Brazil.
authors.forename: Marcelo Aparecido
authors.initials: MA
authors.lastname: da Silva
authors.forename: Claudia Andréa Lima
authors.initials: CA
authors.lastname: Cardoso
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Lourdes Campaner
authors.initials: LC
authors.lastname: dos Santos
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D039262:Eriocaulaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005419:Flavonoids; D010936:Plant Extracts