Triterpenes from Warszewiczia coccinea (Rubiaceae) as inhibitors of acetylcholinesterase.
Seventy-six plant extracts from the Panamanian flora have been screened for acetylcholinesterase (AChE) inhibitors by thin-layer chromatography (TLC) bioautography. The most promising extracts with AChE inhibitory and free radical scavenging activities at 100 microg were those of Tabernaemontana panamensis (Markgr., Boiteau & L. Allorge) Leeuwenb., Pentagonia macrophylla Benth., and Warszewiczia coccinea (Vahl) Klotzsch. Bioguided fractionation of W. coccinea stem extract afforded two triterpenes, 3beta,6beta,19alpha-trihydroxy-urs-12-en-28-oic acid (1) and 3beta,6beta-dihydroxy-olean-12-en-28-oic acid (sumaresinolic acid) (2), with AChE inhibitory activity. Their structures were determined by spectroscopic methods. This is the first report of these bioactive triterpenes in W. coccinea.
Calderón
Angela I
AI
Department of Pharmacal Sciences, Harrison School of Pharmacy, Auburn University, 4306B Walker Building, Auburn, AL 36849, USA.
Simithy
Johayra
J
Quaggio
Giuliana
G
Espinosa
Alex
A
López-Pérez
Jose Luis
JL
Gupta
Mahabir P
MP
D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D019666:Rubiaceae / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D014315:Triterpenes; D009828:Oleanolic Acid; C545911:3beta,6beta-dihydroxyolean-12-en-28-oic acid