[Effects of ADAM28 AS-ODN on proliferation, differentiation and apoptosis of HPDLSC].
OBJECTIVE
To investigate the effects of a disintegrin and metalloproteinase 28(ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human periodontal ligament stem cell(HPDLSC) and possible mechanism.


METHODS
HPDLSC was isolated, cultured in vitro and identified. Synthetic ADAM28 AS-ODN and S-ODN were transfected into HPDLSC, respectively. MTT chromatometry, enzyme dynamics and flow cytometry (FCM) were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HPDLSC. Statistical significance was assessed by the Student-Newman-Keuls (SNK) test with SPSS 13.0 software package.


RESULTS
In ADAM28 AS-ODN group, the proliferation activity and index of HPDLSC were significantly lower than those of S-ODN group and untransfected group, alkaline phosphatase(ALP) secretion level and percentage of apoptotic cells went up obviously. Significant difference was detected between AS-ODN group and other groups (P<0.01).


CONCLUSIONS
ADAM28 AS-ODN could inhibit HPDLSC proliferation and influence the changes of cell cycle, promote ALP secretion and induce HPDLSC apoptosis significantly.
Zhao
Zheng
Z
Institute of Stomatology, General Hospital of Chinese PLA, Beijing, China. zhaozheng170@sohu.com
Liu
Hong-chen
HC
Huang
Zheng-nan
ZN
D051722:ADAM Proteins; D000469:Alkaline Phosphatase; D017209:Apoptosis; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D016376:Oligonucleotides, Antisense; D010513:Periodontal Ligament; D013234:Stem Cells; D014162:Transfection
D016376:Oligonucleotides, Antisense; D000469:Alkaline Phosphatase; D051722:ADAM Proteins; C410224:ADAM28 protein, human