title: Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids.
abstract: To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Zhan
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhou
authors.forename: W G
authors.initials: WG
authors.lastname: Shan
mesh_terms: D004795:Enzyme Stability; D006358:Hot Temperature; D006801:Humans; D019787:Linoleic Acid / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*; D017962:alpha-Linolenic Acid / Q000737:chemistry*
chemical_list: D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D013482:Superoxide Dismutase