[Induction of hepatic specification of human adipose-derived stem cells (hADSCs) in vitro].
OBJECTIVE
To induce hepatic differentiation of human adipose-derived stem cells (hADSCs) in vitro.


METHODS
hADSCs were isolated from human adipose tissue and treated with improved hepatic medium containing HGF, bFGF and FGF4. After 7 days of culture, OSM was added to the culture media. Cell growth during hepatic differentiation was evaluated by CCK8 assay. Morphology of differentiation was examined under light microscope. Liver specific genes and proteins were detected by RT-PCR analysis and immunohistochemical staining, respectively. And functional characteristics of hepatocytes were also examined.


RESULTS
The number of hADSCs cultured in the improved hepatic media was increased significantly in comparison to hADSCs cultured in control media from 5 days to 21 days (t=6.59, 8.69, 15.94 and 24.64, respectively, P<0.05). The hADSCs-derived hepatocyte-like cells exhibited hepatocyte morphology, expressed hepatocyte markers, possessed hepatocyte-specific activities, such as uptake and excretion of indocyanine green, glycogen storage and albumin production.


CONCLUSIONS
hADSCs can be induced into hepatocyte-like cells in this differentiation system. And this differentiation system promoted the growth of hADSCs.
Wang
Min
M
Department of Stem Cell and Regenerative Medicine, Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Pei
Hai-yun
HY
Guan
Li-dong
LD
Nan
Xue
X
Bai
Ci-xian
CX
Liu
Hui
H
Li
Bao-wei
BW
Wang
Yun-fang
YF
Pei
Xue-tao
XT
D000273:Adipose Tissue / Q000166:cytology*; D000418:Albumins / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000509:alpha-Fetoproteins / Q000378:metabolism
D000418:Albumins; D003470:Culture Media; D000509:alpha-Fetoproteins; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor