Polymer migration among phospholipid liposomes.
Complexation of phospholipid lipsomes with a cationic polymer, poly(N-ethyl-4-vinylpyridinium bromide) (PEVP), and subsequent interliposomal migration of the adsorbed macromolecules, have been investigated. Liposomes of two different charge types were examined: (a) a liposomal system, with an overall charge near zero, consisting of zwitterionic phosphatidylcholine (egg lecithin, EL) with added doubly anionic phospholipid, cardiolipin (CL(2-)), and cationic dihexadecyldimethylammonium bromide (HMAB(+)), in a CL(2-)/HMAB(+) charge-to-charge ratio of 1:1; (b) an anionic liposomal system composed of an EL/CL(2-) mixture plus polyoxyethylene monocetyl ether (Brij 58). Both three-component systems were designed specifically to preclude liposomal aggregation upon electrostatic association with the PEVP, a phenomenon that had complicated analysis of data from several two-component liposomes. PEVP macromolecules were found from fluorescence experiments to migrate among the charge-neutral EL/CL(2-)/HMAB(+) liposomes. In the case of anionic EL/CL(2-)/Brij liposomes, a combination of fluorescence and laser microelectrophoresis methods showed that PEVP macromolecules travel from liposome to liposome while being electrostatically associated with anionic lipids.
Davydov
Dmitry A
DA
Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, 1-3, 119992 Moscow, Russian Federation.
Yaroslavova
Ekaterina G
EG
Rakhnyanskaya
Anna A
AA
Efimova
Anna A
AA
Ermakov
Yury A
YA
Menger
Fredric M
FM
Yaroslavov
Alexander A
AA
D002308:Cardiolipins; D008081:Liposomes / Q000737:chemistry*; D008956:Models, Chemical; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
D002308:Cardiolipins; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C046417:dihexadecyldimethylammonium