title: Lion (Panthera leo) and cheetah (Acinonyx jubatus) IFN-gamma sequences.
abstract: Cloning and sequencing of the full length lion and cheetah interferon-gamma (IFN-gamma) transcript will enable the expression of the recombinant cytokine, to be used for production of monoclonal antibodies and to set up lion and cheetah-specific IFN-gamma ELISAs. These are relevant in blood-based diagnosis of bovine tuberculosis, an important threat to lions in the Kruger National Park. Alignment of nucleotide and amino acid sequences of lion and cheetah and that of domestic cats showed homologies of 97-100%.
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. M.Maas3@students.uu.nl
authors.forename: Miriam
authors.initials: M
authors.lastname: Maas
authors.forename: Ildiko
authors.initials: I
authors.lastname: Van Rhijn
authors.forename: Maria T E P
authors.initials: MT
authors.lastname: Allsopp
authors.forename: Victor P M G
authors.initials: VP
authors.lastname: Rutten
mesh_terms: D002612:Acinonyx / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007371:Interferon-gamma / Q000235:genetics*; D008045:Lions / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013019:South Africa; D013045:Species Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000635:transmission
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007371:Interferon-gamma