Quantification of host-microbe interactions by automated fluorescence microscopy.
We describe an automated fluorescence microscopy-based assay that quantifies the invasion of mammalian cells by intracellular pathogens. Pathogens associated with host cell surfaces, intracellular pathogens and mammalian cells are directly counted based on their specific fluorescent labeling. Such approach utilizes automated image acquisition and processing, and is thus ideally suited for high-throughput analyses. This method was validated using Listeria monocytogenes as a model intracellular pathogen.
Haghighat
Anne-Cécile
AC
Departments of Microbiology and Internal Medicine, Division of Infectious Diseases, Center for Microbial Interface Biology, Ohio State University, Columbus, OH, USA.
Seveau
Stéphanie
S
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D001330:Electronic Data Processing; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D005260:Female; D005456:Fluorescent Dyes; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008089:Listeria monocytogenes / Q000166:cytology* / Q000472:pathogenicity; D008088:Listeriosis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling
D005456:Fluorescent Dyes