Matched cultures of keratinocytes and fibroblasts derived from normal and NER-deficient mouse models.
The uppermost layer of our skin, the epidermis, is formed largely of keratinocytes which constitute the skin's major barrier function and the first line of defence against environmental physical, chemical and biological agents. The subsequent layer, the dermis, which is mainly formed by fibroblasts, has a more supportive function, containing large amounts of collagen, blood vessels and nerve endings and is less directly affected by external insults. Hence it is likely that keratinocytes and fibroblasts have evolved different strategies to cope with the dangers of the environment. Mouse models with various genetic backgrounds in genome care-taking systems, such as DNA repair processes, are well suited to study differences between these two cell types and their implications for cancer and aging. In this chapter we describe a simple procedure to establish long-term keratinocyte and fibroblast cultures from, respectively, the epidermis and dermis of normal or NER-deficient newborn mice. The importance of the external O(2) pressure during the establishment and maintenance of these matched cultures is discussed.
Pines
Alex
A
Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden, The Netherlands.
Backendorf
Claude
C
D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods*; D004260:DNA Repair; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D023822:Tissue Engineering