title: H2S signals through protein S-sulfhydration.
abstract: Hydrogen sulfide (H2S), a messenger molecule generated by cystathionine gamma-lyase, acts as a physiologic vasorelaxant. Mechanisms whereby H2S signals have been elusive. We now show that H2S physiologically modifies cysteines in a large number of proteins by S-sulfhydration. About 10 to 25% of many liver proteins, including actin, tubulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are sulfhydrated under physiological conditions. Sulfhydration augments GAPDH activity and enhances actin polymerization. Sulfhydration thus appears to be a physiologic posttranslational modification for proteins.
authors.affiliation: Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Asif K
authors.initials: AK
authors.lastname: Mustafa
authors.forename: Moataz M
authors.initials: MM
authors.lastname: Gadalla
authors.forename: Nilkantha
authors.initials: N
authors.lastname: Sen
authors.forename: Seyun
authors.initials: S
authors.lastname: Kim
authors.forename: Weitong
authors.initials: W
authors.lastname: Mu
authors.forename: Sadia K
authors.initials: SK
authors.lastname: Gazi
authors.forename: Roxanne K
authors.initials: RK
authors.lastname: Barrow
authors.forename: Guangdong
authors.initials: G
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Snyder
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001704:Biopolymers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D051379:Mice; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D001704:Biopolymers; D013438:Sulfhydryl Compounds; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006862:Hydrogen Sulfide