Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms.
The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.
Holochová
Pavla
P
Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 61137 Brno, Czech Republic.
Růzicková
Vladislava
V
Dostálová
Lucie
L
Pantůcek
Roman
R
Petrás
Petr
P
Doskar
Jirí
J
D015373:Bacterial Typing Techniques / Q000379:methods; D003604:Czechoslovakia; D005083:Exfoliatins / Q000235:genetics*; D005260:Female; D006771:Hospitals, Maternity; D006801:Humans; D007231:Infant, Newborn; D010392:Pemphigus / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D039002:Prophages / Q000235:genetics*; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
D005083:Exfoliatins