title: Osteogenic activity of MG63 cells on bone-like hydroxyapatite/collagen nanocomposite sponges.
abstract: The hydroxyapatite/collagen (HAp/Col) sponge with 95% (v/v) porosity was prepared by freeze-drying of a HAp/Col fiber suspension. MG63 cells were seeded onto the HAp/Col sponge and cultured under a pressure/perfusion condition with osteogenic supplements. A collagen (Col) sponge was used as a control. The cells with sponge were examined by a histology, total DNA content and gene expression. The cells showed good attachment and proliferation everywhere in the HAp/Col sponge, while the cells mainly proliferated at the peripheral part of the Col sponge. Thus, total DNA content in the HAp/Col sponges reached 1.8 times greater than that in the Col sponges at Day 21. Further, the cells and extracellular matrix only in the HAp/Col sponge were calcified, although the cells in both sponge evenly expressed osteogenic gene. These results suggest that the HAp/Col sponge could be useful as a scaffold for bone tissue engineering.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Teruaki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masanori
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Koyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takakuda
mesh_terms: D020341:Absorbable Implants; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000295:instrumentation; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008954:Models, Biological; D053761:Nanocomposites / Q000737:chemistry; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite