title: Curcumin nutrition for the prevention of mesenteric ischemia-reperfusion injury: an experimental rodent model.
abstract: BACKGROUND
  Curcumin is an anti-oxidant molecule known to be a potent inhibitor of nuclear factor-kappaB (NF-kappaB). It has been shown to attenuate ischemia/reperfusion (I/R) injury in several organ systems. In this study, we sought to investigate the effects of curcumin on the prevention of superior mesenteric artery I/R injury in rats.
  
  
  METHODS
  Wistar albino rats were randomly allocated to 3 groups: group I, sham operated (n = 10); group II, I/R injury only (n = 10); group III, curcumin-treated I/R cohort (n = 10). Group I animals underwent laparotomy without I/R injury. After group II animals underwent laparotomy, 60 minutes of superior mesenteric artery ligation were followed by 3 hours of reperfusion. In the curcumin group, 15 days before I/R, curcumin (40 mg/kg) was administered by gastric gavage. All animals were sacrificed at the end of reperfusion. Intestinal tissue samples were obtained to investigate intestinal mucosal injury; in addition we estimated levels of myeloperoxidase (MPO) activity, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha.
  
  
  RESULTS
  There were statistically significant decreases in GSH levels, along with an increase in intestinal mucosal injury scores, MPO activity, MDA levels, NO, IL-6, and TNF-alpha in group I when compared with groups II and III (P = .01). Curcumin treatment in group III produced a significant increase in GSH levels, as well as a decrease in intestinal mucosal injury scores, MPO activity, MDA, and NO levels when compared with group II (P < .05).
  
  
  CONCLUSIONS
  This study showed that curcumin treatment significantly attenuated reperfusion injury in a superior mesenteric artery I/R model in rats.
authors.affiliation: Okmeydani Training and Research Hospital, Department of General Surgery, Istanbul, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Karatepe
authors.forename: O B
authors.initials: OB
authors.lastname: Gulcicek
authors.forename: M
authors.initials: M
authors.lastname: Ugurlucan
authors.forename: G
authors.initials: G
authors.lastname: Adas
authors.forename: M
authors.initials: M
authors.lastname: Battal
authors.forename: A
authors.initials: A
authors.lastname: Kemik
authors.forename: G
authors.initials: G
authors.lastname: Kamali
authors.forename: T
authors.initials: T
authors.lastname: Altug
authors.forename: S
authors.initials: S
authors.lastname: Karahan
mesh_terms: D000818:Animals; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005751:Gastric Lavage; D005978:Glutathione / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013152:Splanchnic Circulation / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione; D003474:Curcumin