title: NO reactions with sol-gel and solution phase samples of the ferric nitrite derivative of HbA.
abstract: The reaction of nitric oxide (NO) with the ferric (met) nitrite derivative of human adult hemoglobin Hb is probed for both solution phase and sol-gel encapsulated populations. The evolution of both the Q band absorption spectrum and fitted populations of Hb derivatives are used to show the sequence of events occurring when NO interacts with nitrite bound to a ferric heme in Hb. The sol-gel is used to compare the evolving populations as a function of quaternary state for the starting met-nitrite populations. The redox status of intermediates is probed using the CN(-) anion to trap ferric heme species. The emergent presence of reactive NO species such as N(2)O(3) during the course of the reaction is probed using the fluorescent probe DAF-2 whereas the fluorophore Chemifluor is used as an indirect measure of the ability of the reaction to create S-nitrosothiols on glutathione. The results are consistent with the formation of a stable reactive intermediate capable of generating bioactive forms of NO. The patterns observed are consistent with a proposed mechanism whereby NO reacts with the ferric nitrite derivative to generate N(2)O(3).
authors.affiliation: Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Camille J
authors.initials: CJ
authors.lastname: Roche
authors.forename: Joel M
authors.initials: JM
authors.lastname: Friedman
mesh_terms: D000328:Adult; D005782:Gels / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D009573:Nitrites / Q000737:chemistry* / Q000378:metabolism; D009589:Nitrogen Oxides / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D012996:Solutions
chemical_list: D005782:Gels; D006454:Hemoglobins; D009573:Nitrites; D009589:Nitrogen Oxides; D012996:Solutions; C031701:nitrogen trioxide; D009569:Nitric Oxide; D007501:Iron