Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo.
Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe3O4, and group D received DNR-loaded MNPs-Fe3O4. For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe3O4 can overcome MDR in vivo.
Chen
Bao-an
BA
Department of Hematology.
Lai
Bin-bin
BB
Cheng
Jian
J
Xia
Guo-hua
GH
Gao
Feng
F
Xu
Wen-lin
WL
Ding
Jia-hua
JH
Gao
Chong
C
Sun
Xin-chen
XC
Xu
Cui-rong
CR
Chen
Wen-ji
WJ
Chen
Ning-na
NN
Liu
Li-jie
LJ
Li
Xiao-mao
XM
Wang
Xue-mei
XM
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003630:Daunorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D052203:Ferrosoferric Oxide / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D007942:Leukemia, Experimental / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D008280:Magnetics; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D051379:Mice; D008819:Mice, Nude; D050997:Nanomedicine; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000235:genetics; D014183:Transplantation, Heterologous
D000903:Antibiotics, Antineoplastic; D017931:DNA Primers; D012334:RNA, Neoplasm; D052203:Ferrosoferric Oxide; D003630:Daunorubicin