title: Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum.
abstract: Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA arrays by suppression subtractive hybridization were generated. cDNAs of floral buds at different stages before flower opening and of leaves at the seedling stage were used. The macroarray analyses of expression profiles of isolated floral genes showed that 157 genes out of the 612 unique ESTs tested revealed higher transcript abundance in the floral buds and uppermost leaves. Among them, 78 genes were determined to be differentially expressed in the perianth, 62 in the stamens, and 100 genes in the carpels. Quantitative real-time PCR of selected genes validated the macroarray results. Remarkably, APETALA3 (AP3) B-class genes isolated from A. caudigerum were upregulated in the perianth, stamens and carpels, implying that the expression domain of B-class genes in this basal angiosperm was broader than those in their eudicot counterparts.
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 650204 Kunming, China. yhzhao808@163.com
authors.forename: Yin-He
authors.initials: YH
authors.lastname: Zhao
authors.forename: Michael
authors.initials: M
authors.lastname: Möller
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Yang
authors.forename: Ting-Song
authors.initials: TS
authors.lastname: Liu
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Zhao
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Dong
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Zhang
authors.forename: Cheng-Yun
authors.initials: CY
authors.lastname: Li
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Wang
authors.forename: De-Zhu
authors.initials: DZ
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D030023:Asarum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D005075:Biological Evolution; D017124:Conserved Sequence; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D026161:MADS Domain Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015854:Up-Regulation / Q000235:genetics
chemical_list: D026161:MADS Domain Proteins; D010940:Plant Proteins; D012333:RNA, Messenger