title: Oncostatin M (OSM) is increased in asthma with incompletely reversible airflow obstruction.
abstract: Oncostatin M, a unique member of the interleukin (IL)-6 cytokine family, is thought to be involved in airway remodeling. The expression of oncostatin M in the lower airways is unknown. The aim of this study was to measure the sputum expression of oncostatin M in patients with asthma with and without irreversible airflow obstruction. Induced sputum was collected from nonsmoking adults with stable asthma (n = 53), 31 with incomplete reversibility of airflow obstruction. Peripheral blood cells were isolated and stimulated with lipopolysaccharide in 10 participants with asthma and irreversible airflow obstruction. Oncostatin M protein levels were determined in supernatant, whereas RNA was extracted to determine Oncostatin M mRNA expression using real-time polymerase chain reaction (PCR). Oncostatin M mRNA expression and protein levels were significantly higher in the sputum of asthmatics with irreversible airflow obstruction. Sputum oncostatin M levels were highest in people with severe airflow obstruction and were localized to airway neutrophils and macrophages. Peripheral blood neutrophils released more oncostatin M when stimulated with lipopolysaccharide compared with unstimulated neutrophils. Sputum oncostatin M is increased in asthma with irreversible airflow obstruction and is present in airway neutrophils and macrophages. Oncostatin M may link airway inflammation to remodeling in asthma.
authors.affiliation: Centre for Asthma and Respiratory Disease, School of Medicine and Public Health, The University of Newcastle, Callaghan NSW, Australia. Jodie.simpson@hnehealth.nsw.gov.au
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Simpson
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Baines
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Boyle
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Scott
authors.forename: Peter G
authors.initials: PG
authors.lastname: Gibson
mesh_terms: D000368:Aged; D000402:Airway Obstruction / Q000473:pathology; D056151:Airway Remodeling; D001249:Asthma / Q000150:complications* / Q000473:pathology; D001773:Blood Cells; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry; D053683:Oncostatin M / Q000032:analysis* / Q000097:blood; D012333:RNA, Messenger / Q000032:analysis; D013183:Sputum / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D053683:Oncostatin M