title: In vitro biological response to a self-adhesive resin cement under different curing strategies.
abstract: Self-adhesive resin cements represents a new approach in indirect restorative dentistry, although little is known about its biological properties. The influence of the curing strategy on the biological response of a self-adhesive resin cement (Rely-X Unicem/3M ESPE) was evaluated through methyltetrazolium (MTT) and nitric oxide (NOx) assays. Cylindrical specimens (n = 48) were prepared and cured according to the following strategies: photoactivation with halogen light-curing unit (LCU) Demetron/Kerr, LED LCU Radii/SDI, and chemical activation. A control group with culture medium and a group with lipopolysaccharide, employed for cells stimulation, were used for comparisons (n = 8). After 24 h of direct contact between specimens and rat alveolar macrophages, the biological response was evaluated. Statistical evaluation of the data was performed with Kruskal-Wallis and Dunn's test (p = 0.05). The MTT test showed that the specimens cured by halogen light and chemical activation provided higher alterations on cell metabolism. For LED-cured specimens, cell viability was compatible to the results observed in the control group. For the NOx assay, all curing methods were cytotoxic to rat macrophages. Rely-X Unicem demonstrated different cytotoxic effects according to the curing strategy employed.
authors.affiliation: Department of Restorative Dentistry, Dental School of the University of Pernambuco-FOP/UPE, Camaragibe-PE, Brazil. gabrielaqueiroz@terra.com.br
authors.forename: Gabriela Q M
authors.initials: GQ
authors.lastname: Monteiro
authors.forename: Fábio B
authors.initials: FB
authors.lastname: Souza
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Pedrosa
authors.forename: Germana C F
authors.initials: GC
authors.lastname: Sales
authors.forename: Célia Maria M B
authors.initials: CM
authors.lastname: Castro
authors.forename: Simone N
authors.initials: SN
authors.lastname: Fraga
authors.forename: Bruno H A
authors.initials: BH
authors.lastname: Galvão
authors.forename: Rodivan
authors.initials: R
authors.lastname: Braz
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D008027:Light; D008070:Lipopolysaccharides / Q000737:chemistry; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry; D051381:Rats; D019279:Resin Cements / Q000009:adverse effects* / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014014:Tissue Adhesives / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D003470:Culture Media; D008070:Lipopolysaccharides; D019279:Resin Cements; D013778:Tetrazolium Salts; D013844:Thiazoles; D014014:Tissue Adhesives; D009569:Nitric Oxide; C022616:thiazolyl blue