[Present and future of reverse genetics of rotavirus].
Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.
Taniguchi
Koki
K
Department of Virology and Parasitology, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. kokitani@fujita-hu.ac.jp
Komoto
Satoshi
S
Sasaki
Jun
J
Kugita
Masanori
M
D000208:Acute Disease; D000818:Animals; D036022:Capsid Proteins; D000939:Epitopes; D005544:Forecasting; D005759:Gastroenteritis / Q000821:virology; D005821:Genetic Techniques / Q000639:trends*; D006801:Humans; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections
D036022:Capsid Proteins; D000939:Epitopes; C060339:VP4 protein, Rotavirus