[Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells].
OBJECTIVE
To evaluate the biological effects of Drynaria fortunei naringin on the proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLC).


METHODS
hPDLC were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to observe the proliferation of hPDLC with the effects of different concentrations of Drynaria fortunei naringin at difference times. The method recommended by International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) was adopted to investigate the effect of Drynaria fortune naringin on the alkaline phosphatase (ALP) activity of hPDLC. At the same time, we used bright blue method to detect the contents of protein in each sample. Then ALP activity in per milligram protein was accounted.


RESULTS
Significant proliferative promotion to hPDLC by Drynaria fortunei naringin could be observed at the dose of 10, 1, 0.1 mg x L(-1). Significant ALP activity of hPDLC promotion of Drynaria fortune naringin could be observed at the dose of 100, 10, 1, 0.1 mg x L(-1) and the dose of 1 mg x L(-1) Drynaria fortune naringin had greatest promotion on ALP activity of hPDLC.


CONCLUSIONS
Drynaria fortune naringin might significantly promote the proliferation and increase the ALP activity of hPDLC.
Jiang
Jun-qiang
JQ
Dept. of Oral Medicine, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China.
Ding
Yi
Y
Li
Xiao-yu
XY
Cai
Wei
W
Wang
Zhong-chao
ZC
D000469:Alkaline Phosphatase; D049109:Cell Proliferation; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D006801:Humans; D010006:Osteoblasts; D010513:Periodontal Ligament; D029621:Polypodiaceae
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D000469:Alkaline Phosphatase; C005274:naringin