title: The effects of pulsed inductively coupled plasma (PICP) on physical properties and biocompatibility of crosslinked gelatin films.
abstract: In this work, pulsed inductively coupled plasma (PICP) device is introduced to treat crosslinked gelatin film. The effects of plasma on the properties of gelatin film were investigated. Type A gelatin film crosslinked by dehydrothermal process was treated by PICP. The properties of crosslinked gelatin were characterized by differential scanning calorimetry (DSC), amino acid content assay (TNBS), contact angle measurement and atomic force microscopy (AFM). The results showed that pulsed inductively coupled plasma did not significantly affect the thermal behavior and the degree of crosslinking of crosslinked gelatin film. The contact angle by both water and ethylene glycol of crosslinked gelatin films treated with nitrogen plasma was decreased in comparison with untreated film. The surface energy was slightly increased when increasing number of repeated discharges were applied from 1 to 20 times. This implied that nitrogen plasma could improve hydrophilicity of the crosslinked gelatin surface. The result from AFM revealed that surface roughness of crosslinked gelatin film was introduced when PICP treatment was applied. In vitro test using L929 mouse fibroblast revealed that, the number of cells proliferated on PICP-treated samples was higher than that on untreated samples. The results indicated that PICP is a potential method for crosslinked gelatin surface modification for future tissue engineering applications.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Isarawut
authors.initials: I
authors.lastname: Prasertsung
authors.forename: Rattachat
authors.initials: R
authors.lastname: Mongkolnavin
authors.forename: Sorada
authors.initials: S
authors.lastname: Kanokpanont
authors.forename: Siriporn
authors.initials: S
authors.lastname: Damrongsakkul
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D019855:Ethylene Glycol / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D055585:Physical Phenomena; D013499:Surface Properties / Q000187:drug effects; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D014867:Water; D005780:Gelatin; D019855:Ethylene Glycol