Trace quantification of 1-octacosanol and 1-triacontanol and their main metabolites in plasma by liquid-liquid extraction coupled with gas chromatography-mass spectrometry.
A method for the simultaneous determination of 1-octacosanol and 1-triacontanol and their main metabolites in rat plasma was developed. The procedure involved ethanolic NaOH saponification of the sample, acidification, liquid-liquid extraction, and derivatization of the analytes to its trimethylsilylether/ester, followed analysis by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. Quantification was performed by the internal standard method using betulin. The method had a good linearity over the range 8.4-540ng/ml (r>or=0.998) and showed an excellent intra-day (R.S.D.=0.59-3.06%) and inter-day (R.S.D.=2.99-5.22%) precision according to the acceptance criteria. The detection limits ranged between 1.32 and 3.47ng/ml. The method was applied successfully to study the total plasmatic concentration of 1-octacosanol, octacosanoic acid, 1-triacontanol, and triacontanoic acid, after an oral dose of policosanols mixture, using plasma samples of 100microl.
Haim
Daniela
D
Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago-7, Chile.
Berríos
Miriam
M
Valenzuela
Alfonso
A
Videla
Luis A
LA
D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D005233:Fatty Alcohols / Q000097:blood* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D005233:Fatty Alcohols; C047578:1-triacontanol; C044309:1-octacosanol