title: Exploring the dihydrodipicolinate synthase tetramer: how resilient is the dimer-dimer interface?
abstract: Dihydrodipicolinate synthase (DHDPS, E.C. 4.2.1.52) is a tetrameric enzyme that catalyses the first committed step of the lysine biosynthetic pathway. Dimeric variants of DHDPS have impaired catalytic activity due to aberrant protein motions within the dimer unit. Thus, it is thought that the tetrameric structure functions to restrict these motions and optimise enzyme dynamics for catalysis. Despite the importance of dimer-dimer association, the interface between subunits of each dimer is small, accounting for only 4.3% of the total monomer surface area, and the structure of the interface is not conserved across species. We have probed the tolerance of dimer-dimer association to mutation by introducing amino acid substitutions within the interface. All point mutations resulted in destabilisation of the 'dimer of dimers' tetrameric structure. Both the position of the mutation in the interface and the physico-chemical nature of the substitution appeared to effect tetramerisation. Despite only weak destabilisation of the tetramer by some mutations, catalytic activity was reduced to approximately 10-15% of the wild-type in all cases, suggesting that the dimer-dimer interface is finely tuned to optimise function.
authors.affiliation: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia. mgriffin@unimelb.edu.au
authors.forename: Michael D W
authors.initials: MD
authors.lastname: Griffin
authors.forename: Renwick C J
authors.initials: RC
authors.lastname: Dobson
authors.forename: Juliet A
authors.initials: JA
authors.lastname: Gerrard
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Perugini
mesh_terms: D055162:Biocatalysis; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D019281:Dimerization; D004795:Enzyme Stability; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
chemical_list: D001704:Biopolymers; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase