Expression of matrix metalloproteinase-9 and significance of a macrophage assay in eosinophilic granuloma.
Although most cases of eosinophilic granuloma (an immune-mediated lesion) do not behave in an aggressive manner, occasionally, recurrence after treatment has been observed. This study aims to investigate the significance and relationship of matrix metalloproteinase-9 (MMP-9) and infiltration of macrophages in eosinophilic granulomas (EGs). The immunohistochemical strept avidin-biotin complex method was used to detect the expression of MMP-9 and CD-68 (for labeling macrophages) in 13 cases of EG. Three of whom showed local recurrence. The results showed that many of the Langerhans histiocytic cells, which reacted positively with MMP-9 protein antibody, were immunolabeled with CD-68 antibody. The results showed that there is a highly significant relationship between the expression of MMP-9 and macrophage count in EGs (P < .001), with recurrent lesions showing a higher mean expression of both MMP-9 and CD-68. In conclusion, there may be a cooperation between macrophages and MMP-9 in EGs, which correlates well with local recurrence. Their expression may therefore provide some prognostic indication of the possible aggressive and recurrence behavior of this lesion.
Zyada
Manal Mohamed
MM
Faculty of Dentistry, Oral Pathology Department, Mansoura University, 35516 Mansoura, Egypt. zyadam@yahoo.com
D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D002452:Cell Count; D004803:Eosinophilic Granuloma / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D020780:Matrix Metalloproteinase 9; C067980:CD68 antigen, human; D000097382:CD68 Molecule