title: Implication of oxidative stress and its correlation with activity of matrix metalloproteinases in patients with Takayasu's arteritis disease.
abstract: BACKGROUND
  Oxidative stress and activation of matrix metalloproteinases (MMPs) together have been widely implicated in various inflammatory vascular disorders. As Takayasu's arteritis (TA) is an inflammatory disorder characterized by destruction of elastic fibers, we hypothesized that oxidative stress and MMPs might play an important role in pathophysiology of the disease.
  
  
  METHODS
  40 patients with clinically defined TA and equal number of normal, healthy, controls were enrolled in the study. Subjects with TA were further divided into clinically active disease or in remission phase of the disease.
  
  
  RESULTS
  Augmented levels of 8-isoPGF(2α) (238.9±125.7 vs 79.5±29.1 ng/ml), nitrite (NO(2)(-)) (14.9±15.6 vs 3.3±1.7 μM) were observed in TA patients as compared to the controls (p<0.01). Further, 8-iso-PGF(2α) (272.2±117.3 vs 105.9±43.5 ng/ml) and NO(2)(-) (17.9±16.1 vs 3.1±2.5 μM) levels were higher in subjects with active disease vs those in remission (p<0.05). Zymography pattern revealed two bands in the range of 85-92 kDa (MMP-9) and 65-72 kDa (MMP-2). Total gelatinolytic activity was found to be significantly higher in subjects with TA as compared to the controls (p<0.05). Gelatinolytic activity was moderately low in controls and subjects in remission as compared to those with active disease (p>0.05). Further, gelatinolytic activity showed a positive correlation with 8-iso-PGF(2α) and NO levels in patients with TA.
  
  
  CONCLUSIONS
  Increased oxidative stress and MMP activity may play an active role in the progression of Takayasu's arteritis disease.
authors.affiliation: Department of Experimental Medicine & Biotechnology, Postgraduate Institute of Medical Education & Research (PGIMER), Chandigarh-160012, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Mahajan
authors.affiliation: Department of Experimental Medicine & Biotechnology, Postgraduate Institute of Medical Education & Research (PGIMER), Chandigarh-160012, India. Electronic address: veenad2001@yahoo.com.
authors.forename: Veena
authors.initials: V
authors.lastname: Dhawan
authors.affiliation: Department of Experimental Medicine & Biotechnology, Postgraduate Institute of Medical Education & Research (PGIMER), Chandigarh-160012, India.
authors.forename: Sonal
authors.initials: S
authors.lastname: Malik
authors.affiliation: Department of Internal Medicine, Postgraduate Institute of Medical Education & Research (PGIMER), Chandigarh-160012, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Jain
mesh_terms: D000293:Adolescent; D000328:Adult; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D013625:Takayasu Arteritis / Q000201:enzymology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020782:Matrix Metalloproteinases