title: Platelet-lysate as an autologous alternative for fetal bovine serum in cardiovascular tissue engineering.
abstract: There is an ongoing search for alternative tissue culture sera to engineer autologous tissues, since use of fetal bovine serum (FBS) is limited under Good Tissue Practice guidelines. We compared FBS with human platelet-lysate (PL) in media for in vitro cell culture. A threefold increase in duplication rate was found when human, saphenous vein-derived myofibroblasts were cultured in PL, whereas expression of marker proteins (alpha-smooth muscle actin, vimentin, desmin, and nonmuscle myosin heavy chain) was similar. Heat shock protein 47 mRNA expression was increased in PL cells, and type III collagen fibers were seen on PL-cell monolayers but not on cells cultured in FBS. These results imply a more efficient collagen fiber production. We also found higher levels of proteins involved in tissue repair and collagen remodeling, which could explain increased production of proteases and protease inhibitors by PL cells. Our findings indicate that PL is beneficial due to the increased duplication rate, in addition to the increased matrix production and remodeling. This could lead to production of strong tissue with properly organized collagen fibers, which is important for heart valve tissue engineering.
authors.affiliation: Department of Cardio-Thoracic Surgery, University Medical Center Utrecht , Utrecht, The Netherlands. priemvis@umcutrecht.nl
authors.forename: Paul W
authors.initials: PW
authors.lastname: Riem Vis
authors.forename: Carlijn V C
authors.initials: CV
authors.lastname: Bouten
authors.forename: Joost P G
authors.initials: JP
authors.lastname: Sluijter
authors.forename: Gerard
authors.initials: G
authors.lastname: Pasterkamp
authors.forename: Lex A
authors.initials: LA
authors.lastname: van Herwerden
authors.forename: Jolanda
authors.initials: J
authors.lastname: Kluin
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D001705:Bioprosthesis; D001792:Blood Platelets; D002319:Cardiovascular System; D002417:Cattle; D002469:Cell Separation; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D017931:DNA Primers / Q000235:genetics; D005312:Fetal Blood; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics; D006350:Heart Valve Prosthesis; D006461:Hemolysis; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044967:Serum; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers; C439971:COL3A1 protein, human; D024061:Collagen Type III; D003470:Culture Media; D017931:DNA Primers; D050958:HSP47 Heat-Shock Proteins; D012333:RNA, Messenger; C494660:SERPINH1 protein, human