Separation and purification of two flavone glucuronides from Erigeron multiradiatus (Lindl.) Benth with macroporous resins.
Scutellarein-7-O-beta-D-glucuronide (SG) and apigenin-7-O-beta-D-glucuronide (AG) are two major bioactive constituents with known pharmacological effects in Erigeron multiradiatus. In this study, a simple method for preparative separation of the two flavone glucuronides was established with macroporous resins. The performance and adsorption characteristics of eight macroporous resins including AB-8, HPD100, HPD450, HPD600, D100, D101, D141, and D160 have been evaluated. The results confirmed that D141 resin offered the best adsorption and desorption capacities and the highest desorption ratio for the two glucuronides among the tested resins. Sorption isotherms were constructed for D141 resin under optimal ethanol conditions and fitted well to the Freundlich and Langmuir models (R(2) > 0.95). Dynamic adsorption and desorption tests was performed on column packed with D141 resin. After one-run treatment with D141 resin, the two-constituent content in the final product was increased from 2.14% and 1.34% in the crude extract of Erigeron multiradiatus to 24.63% and 18.42% in the final products with the recoveries of 82.5% and 85.4%, respectively. The preparative separation of SG and AG can be easily and effectively achieved via adsorption and desorption on D141 resin, and the method developed can be referenced for large-scale separation and purification of flavone glucuronides from herbal raw materials.
Zhang
Zhi-Feng
ZF
Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China. zhangzhf99@gmail.com
Liu
Yuan
Y
Luo
Pei
P
Zhang
Hao
H
D000327:Adsorption; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification*; D049919:Erigeron / Q000737:chemistry*; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D012996:Solutions; D013696:Temperature
D005965:Glucuronates; D012117:Resins, Synthetic; D012996:Solutions; C456097:apigenin-7-O-glucuronide; C484876:scutellarin; D047310:Apigenin