Satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors.
The coat protein of satellite panicum mosaic virus (SPCP) is known to effectively protect its cognate RNA from deleterious events, and here, we tested its stabilizing potential for heterologous virus-based gene vectors in planta. In support of this, a Potato virus X (PVX) vector carrying the SPMV capsid protein (PVX-SPCP) gene was stable for at least three serial systemic passages through Nicotiana benthamiana. To test the effect of SPCP in trans, PVX-SPCP was co-inoculated onto N. benthamiana together with a Tomato bushy stunt virus (TBSV) vector carrying a green fluorescent protein (GFP) gene that normally does not support systemic GFP expression. In contrast, co-inoculation of TBSV-GFP plus PVX-SPCP resulted in GFP accumulation and concomitant green fluorescent spots in upper, non-inoculated leaves in a temperature-responsive manner. These results suggest that the multifaceted SPMV CP has intriguing effects on virus-host interactions that surface in heterologous systems.
Everett
Anthany L
AL
Department of Plant Pathology and Microbiology, 2132 TAMU, Texas A&M University, College Station, TX 77843, USA.
Scholthof
Herman B
HB
Scholthof
Karen-Beth G
KB
D036022:Capsid Proteins / Q000502:physiology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D009029:Mosaic Viruses / Q000502:physiology*; D008897:Panicum / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D017863:Potexvirus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012526:Satellite Viruses / Q000502:physiology*; D013696:Temperature; D017864:Tombusvirus / Q000235:genetics
D036022:Capsid Proteins; D049452:Green Fluorescent Proteins