Prolonged consumption of soy or fish-oil-enriched diets differentially affects the pattern of hypothalamic neuronal activation induced by refeeding in rats.
We used c-Fos immunoreactivity to estimate neuronal activation in hypothalamic feeding-regulatory areas of 3-month-old rats fed control or oil-enriched diets (soy or fish) since weaning. While no diet effect was observed in c-Fos immunoreactivity of 24-h fasted animals, the acute response to refeeding was modified by both hyperlipidic diets but with different patterns. Upon refeeding, control-diet rats had significantly increased c-Fos immunoreactivity only in the paraventricular hypothalamic nucleus (PVH, 142%). In soy-diet rats, refeeding with the soy diet increased c-Fos immunoreactivity in dorsomedial hypothalamic nucleus (DMH, 271%) and lateral hypothalamic area (LH, 303%). Refeeding fish-diet rats with the fish diet increased c-Fos immunoreactivity in PVH (161%), DMH (177%), VMH (81%), and ARC (127%). Compared to the fish-diet, c-Fos immunoreactivity was increased in LH by the soy-diet while it was decreased in ventromedial hypothalamic nucleus (VMH) and arcuate hypothalamic nucleus (ARC). Based on the known roles of the activated nuclei, it is suggested that, unlike the fish-diet, the soy-diet induced a potentially obesogenic profile, with high LH and low VMH/PVH activation after refeeding.
Watanabe
Regina L H
RL
Dept of Physiology, Division of Nutrition Physiology, Federal University of São Paulo, 04023-060 São Paulo, Brazil.
Andrade
Iracema S
IS
Zemdegs
Juliane C S
JC
Albuquerque
Kelse T
KT
Nascimento
Claudia M O
CM
Oyama
Lila M
LM
Carmo
Maria G T
MG
Nogueira
Maria I
MI
Ribeiro
Eliane B
EB
D000818:Animals; D001835:Body Weight; D004032:Diet; D004435:Eating / Q000502:physiology*; D002149:Energy Intake; D005215:Fasting / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D005395:Fish Oils / Q000737:chemistry; D007031:Hypothalamus / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009928:Organ Specificity; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000737:chemistry
D005227:Fatty Acids; D005395:Fish Oils; D016760:Proto-Oncogene Proteins c-fos; D013024:Soybean Oil