title: Purification, crystallization and preliminary X-ray analysis of beta-glucosidase from Kluyveromyces marxianus NBRC1777.
abstract: The intracellular beta-glucosidase from Kluyveromyces marxianus NBRC1777 (KmBglI) belongs to glycoside hydrolase family 3 and has a unique domain architecture. Selenomethionine-labelled KmBglI was purified and crystallized by the hanging-drop vapour-diffusion method using the purified enzyme at 30 mg ml(-1), 0.04 M potassium dihydrogen phosphate pH 5.1, 16%(w/v) PEG 8000 and 20%(v/v) glycerol. The crystal belonged to space group C2, with unitcell parameters a = 245.8, b = 148.7, c = 119.9 angstrom, beta = 112.9 degrees. Multiple-wavelength anomalous dispersion data were collected at 2.4 and 2.5 angstrom resolution. A tetramer was assumed to be present in the asymmetric unit, which gave a Matthews coefficient of 2.6 angstrom(3) Da(-1).
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Yoshida
authors.forename: Masafumi
authors.initials: M
authors.lastname: Hidaka
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyanagi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Minami
authors.forename: Hisanori
authors.initials: H
authors.lastname: Tamaki
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Takane
authors.initials: T
authors.lastname: Katayama
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D007716:Kluyveromyces / Q000201:enzymology*; D008969:Molecular Sequence Data; D001617:beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D001617:beta-Glucosidase