title: Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column.
abstract: This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorfenvinfos, due to their importance in the field of water policy. The efficiency of insecticide degradation was controlled using a highly sensitive biosensor allowing the detection of OP concentration as low as 0.004 microgL(-1). Under optimum conditions, it was shown that a column incorporating 500IU of PTE was suitable for the detoxification of solutions containing either isolated pesticides or pesticides mixtures, even at concentrations higher than authorized limits. Finally, the method was shown to be adapted to the decontamination of real samples of pesticides with concentrations up to 20 microgL(-1).
authors.affiliation: IMAGES, EA4218, Building S, Université de Perpignan Via Domitia, 66860 Perpignan cedex, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Istamboulie
authors.forename: Romain
authors.initials: R
authors.lastname: Durbiano
authors.forename: Didier
authors.initials: D
authors.lastname: Fournier
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002709:Chlorfenvinphos / Q000032:analysis / Q000378:metabolism*; D004390:Chlorpyrifos / Q000032:analysis / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D007306:Insecticides / Q000032:analysis / Q000378:metabolism*; D044345:Phosphoric Triester Hydrolases / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D007306:Insecticides; D014874:Water Pollutants, Chemical; D044345:Phosphoric Triester Hydrolases; D002709:Chlorfenvinphos; D004390:Chlorpyrifos