title: Development of a novel fluorometric assay for nitric oxide utilizing sesamol and its application to analysis of nitric oxide-releasing drugs.
abstract: Nitric oxide (NO) is related to various physiological effects as well as to numerous diseases caused by accentuation of NO production. Measurement of NO in cells and tissues is difficult as NO readily reacts with other molecules; furthermore, its half-life as a radical is fleeting. Currently, many NO pharmaceuticals are marketed as therapeutic agents for ischemic disease. Consequently, the identification of NO radicals and determination of generation rate from pharmaceuticals is very important when the effect of the medicinal supply is estimated. In this study, we developed a fluorometric assay for NO employing sesamol (3,4-methylenedioxyphenol) as a fluorometric substrate. Sesamol is converted to a fluorescent derivative (ex. 365 nm, em. 447 nm), which is dimmer in the presence of NO. The detection limit of NO with this method is 400 fmol; moreover, NO generated from drugs can be measured.
authors.affiliation: School of Pharmacy, Showa University, Tokyo 142-8555, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Abe
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakabayashi
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Murayama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sano
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Masako
authors.initials: M
authors.lastname: Maeda
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
mesh_terms: D000975:Antioxidants; D052117:Benzodioxoles; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D007511:Ischemia / Q000188:drug therapy; D009569:Nitric Oxide / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D010636:Phenols
chemical_list: D000975:Antioxidants; D052117:Benzodioxoles; D004364:Pharmaceutical Preparations; D010636:Phenols; D009569:Nitric Oxide; C025583:sesamol