Aberrant promoter methylation of FBLN-3 gene and clinicopathological significance in non-small cell lung carcinoma.
FBLN-3 has been identified as an antagonist of angiogenesis which modulates cell morphology, growth, adhesion, and motility. In the present study, we investigated the promoter methylation status of FBLN-3 gene in non-small cell lung carcinoma (NSCLC) by methylation-specific PCR and analyzed its correlation with clinicopathological factors. The methylation of FBLN-3 gene promoter was detected in 28 of 65 (43.1%) NSCLC tissue samples and 6 of 65 (9.2%) corresponding non-tumor tissue samples (P<0.05). The methylation of FBLN-3 gene promoter led to the loss of FBLN-3 gene expression in NSCLC. Additionally, FBLN-3 promoter methylation was observed to be correlated with relative poor differentiation, advanced pathological stage and lymph node metastasis of NSCLC patients (P=0.017, 0.0057 or 0.002, respectively), but not with gender, age, histological type, and smoking condition (P>0.05). These results indicated that the loss of FBLN-3 gene induced by promoter methylation might play important roles in the progression of NSCLC and FBLN-3 promoter methylation might be a promising biomarker for early detection of NSCLC.
Wang
Rui
R
Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, China.
Zhang
You-Wei
YW
Chen
Long-Bang
LB
D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D018450:Disease Progression; D055088:Early Detection of Cancer; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D008207:Lymphatic Metastasis; D008297:Male; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic / Q000235:genetics
C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; D004247:DNA