title: Correlation of expression of human arrest-defective-1 (hARD1) protein with breast cancer.
abstract: Human arrest-defective-1 (hARD1) was reported to be important in regulating cell cycle and promoting lung cancer cell proliferation. Here we have investigated the correlation between hARD1 and breast cancer. Analysis with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry (FCM) demonstrated that overexpression of hARD1 was associated with increased proliferation of MCF-7 cell, a human breast cancer cell line. Western blotting and immunohistochemical staining assay showed that hARD1 presented higher in breast cancer tissue than the adjacent tissue; accumulation of hARD1 protein was higher in 86% (37/43) of breast cancer, far more than noncancer samples. Our results suggest that hARD1 might play an important role in breast cancer carcinogenesis.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Science, Yunnan University, Kunming, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: MingXing
authors.initials: M
authors.lastname: Ma
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: JianHua
authors.initials: J
authors.lastname: Lai
authors.forename: Shan
authors.initials: S
authors.lastname: Yan
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: MingJun
authors.initials: M
authors.lastname: Xiang
authors.forename: DeYong
authors.initials: D
authors.lastname: Tan
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016373:DNA, Antisense; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D063209:N-Terminal Acetyltransferase A; D063213:N-Terminal Acetyltransferase E; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D016373:DNA, Antisense; D000123:Acetyltransferases; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; D063213:N-Terminal Acetyltransferase E