Analysis of short tandem repeats by parallel DNA threading.
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.
Zajac
Pawel
P
Department of Gene Technology, School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden.
Oberg
Christine
C
Ahmadian
Afshin
A
D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008957:Models, Genetic; D009711:Nucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D009711:Nucleotides; D004247:DNA