An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments.
OBJECTIVE
To compare the effects of 2 different media on embryo morphology and development at days 2/3.


METHODS
Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n = 286) or ICSI (n = 390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates.


RESULTS
Fertilization rates in GIII series and ISM (IVF: 59.9 vs 62.0% and ICSI: 65.7 vs 66.8%) respectively were not different. GIII series showed an increase, compared to ISM, of early cleavage rate, (IVF: 25.8 vs 16.2% (p = 0.005); ICSI: 40.8 vs 25.5% (p < 0.0001), and good embryo morphology rate at day 2 [IVF: 64.6 vs 57.3% (p = 0.01); ICSI: 74.2 vs 69.4 (p = 0.03)] and at day 3 [IVF: 57.5 vs 49.0% (p = 0.02); ICSI: 67.2 vs 61.6% (p = 0.01)].


CONCLUSIONS
Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.
Sifer
Christophe
C
Service d'Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction, Centre Hospitalier Universitaire Jean Verdier, Assistance Publique-Hôpitaux de Paris, 93143 Bondy, France. christophe.sifer@jvr.aphp.fr
Handelsman
Deborah
D
Grange
Emilie
E
Porcher
Raphael
R
Poncelet
Christophe
C
Martin-Pont
Brigitte
B
Benzacken
Brigitte
B
Wolf
Jean-Philippe
JP
D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D003470:Culture Media