title: Alanine aminopeptidase and dipeptidyl peptidase IV in saliva of chronic periodontitis patients.
abstract: BACKGROUND
  Alanine aminopeptidase (ALAP) and dipeptidyl peptidase IV (DPPIV) are ectopeptidases that play a role in collagen degradation and are thought to be involved in the destruction of periodontal tissue. This study compared the activities of salivary ALAP and DPPIV in patients with periodontitis and periodontally healthy subjects. The correlations of enzyme activities with clinical variables and the presence of Porphyromonas gingivalis were also evaluated.
  
  
  METHODS
  Whole saliva was collected from 30 periodontally healthy subjects, 30 localized chronic periodontitis (LCP) patients, and 30 generalized chronic periodontitis (GCP) patients to determine the activities of ALAP and DPPIV. The presence of P. gingivalis in subgingival plaque was detected by polymerase chain reaction. Periodontal clinical assessments included probing depth, clinical attachment level, and bleeding on probing.
  
  
  RESULTS
  The activities of DPPIV in the LCP and GCP groups were not significantly different from one another, but both groups had significantly higher enzyme activities than the periodontally healthy group (P = 0.001). DPPIV activity was positively correlated with all clinical parameters and the prevalence of P. gingivalis. The ALAP activities were not significantly different among the three study groups. There was no significant correlation of ALAP activity with any of the clinical and bacterial parameters.
  
  
  CONCLUSIONS
  DPPIV, but not ALAP, activity is associated with periodontitis and the presence of P. gingivalis.
authors.affiliation: Selaphum Hospital, Amphur Selaphum, Roi-Et, Thailand.
authors.forename: Piyamas
authors.initials: P
authors.lastname: Aemaimanan
authors.forename: Nison
authors.initials: N
authors.lastname: Sattayasai
authors.forename: Nawarat
authors.initials: N
authors.lastname: Wara-aswapati
authors.forename: Waranuch
authors.initials: W
authors.lastname: Pitiphat
authors.forename: Waraporn
authors.initials: W
authors.lastname: Suwannarong
authors.forename: Saengsome
authors.initials: S
authors.lastname: Prajaneh
authors.forename: Suwimol
authors.initials: S
authors.lastname: Taweechaisupapong
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D018826:CD13 Antigens / Q000032:analysis*; D055113:Chronic Periodontitis / Q000201:enzymology* / Q000382:microbiology; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis*; D005260:Female; D005884:Gingival Hemorrhage / Q000201:enzymology / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000382:microbiology; D010514:Periodontal Pocket / Q000201:enzymology / Q000382:microbiology; D010519:Periodontium / Q000201:enzymology; D016966:Porphyromonas gingivalis / Q000502:physiology; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012737:Sex Factors; D055815:Young Adult
chemical_list: D012471:Salivary Proteins and Peptides; D018826:CD13 Antigens; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4