High-resolution atomic force microscopy of DNA.
A method using high resolution atomic force microscopy for imaging DNA has been elaborated. Using super-sharp probes and modified graphite as support for molecule adsorption, DNA molecule images were obtained whose resolution made possible the observation of their fine structure with repeated helical motifs. The method can be used to visualize individual spread molecules of single-stranded DNA.
Klinov
D V
DV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. klinov@ibch.ru
Neretina
T V
TV
Prokhorov
V V
VV
Dobrynina
T V
TV
Aldarov
K G
KG
Demin
V V
VV
D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D045647:DNA Glycosylases; D004279:DNA, Viral / Q000032:analysis*; D006108:Graphite / Q000032:analysis; D018625:Microscopy, Atomic Force / Q000379:methods*; D015394:Molecular Structure; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010100:Oxygen / Q000378:metabolism; D012821:Silanes; D013696:Temperature; D013997:Time Factors
D004279:DNA, Viral; D012821:Silanes; D006108:Graphite; D004247:DNA; D045647:DNA Glycosylases; D010100:Oxygen