Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease.
In 2007, the islet community was notified that the collagenase product most commonly used for human islet isolations contained bovine neural tissue contaminants. To minimize this potential hazard, we adapted our human islet processing procedure to use a GMP-manufactured, bovine neural tissue-free collagenase blend. Here, we describe the factors that we consider most important for achieving reproducible and clinically useable islet isolations using this product.
Szot
Gregory L
GL
Department of Internal Medicine, Diabetes Center, University of California-San Francisco, San Francisco, CA, USA.
Lee
Michael R
MR
Tavakol
Mohammad M
MM
Lang
Jiena
J
Dekovic
Florinna
F
Kerlan
Robert K
RK
Stock
Peter G
PG
Posselt
Andrew M
AM
D000328:Adult; D000818:Animals; D002417:Cattle; D017364:Collagenases / Q000592:standards*; D006801:Humans; D007515:Islets of Langerhans / Q000033:anatomy & histology; D016381:Islets of Langerhans Transplantation; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000592:standards*; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards; D009927:Tissue and Organ Procurement
D010447:Peptide Hydrolases; D017364:Collagenases