title: Experimental use of an elastomeric surgical sealant for arterial hemostasis and its long-term tissue response.
abstract: OBJECTIVE
  Reliable suture line hemostasis should improve the outcome of aortic surgery. We examined the hemostatic effect and the tissue response of a novel elastomeric surgical sealant.
  
  
  METHODS
  Using porcine internal carotid arteries, we performed 16 end-to-end anastomoses with four stitches of simple interrupted sutures under full heparinization. The anastomoses were divided into two groups (eight anastomoses per group). Either novel sealant or fibrin glue was applied. The amount of bleeding was measured during the 30 s period after removing the vascular clamp. In a separate experiment, we applied the novel sealant around the abdominal aorta of rabbits (n=6) to assess the effect of the elastomeric property of the sealant on arterial wall histology. For comparison, we applied cyanoacrylate, which has no elastomeric property (n=6). A histological study was performed three months after the operation.
  
  
  RESULTS
  The novel sealant prevented arterial bleeding. The amount of bleeding from the anastomoses applied with novel sealant and fibrin glue was 0.12+/-0.03 g vs. 91.8+/-16.5 g, respectively (P<0.001). Thinning of the rabbit aortic wall was observed in the cyanoacrylate-treated abdominal aorta, whereas no thinning was observed in the novel sealant group. Histological examination revealed neither cell death nor necrosis in the novel sealant group.
  
  
  CONCLUSIONS
  The novel sealant effectively prevented arterial bleeding from the anastomosis under full heparinization. In addition, the elastomeric property of the sealant prevented thinning of the aortic wall. The novel sealant may be a promising hemostatic agent for arterial anastomosis.
authors.affiliation: Department of Cardiovascular Surgery, Kyushu University, Fukuoka, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Oda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Morita
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Tanoue
authors.forename: Masataka
authors.initials: M
authors.lastname: Eto
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Ryuji
authors.initials: R
authors.lastname: Tominaga
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use; D001012:Aorta, Abdominal / Q000187:drug effects / Q000473:pathology; D002343:Carotid Artery, Internal / Q000601:surgery*; D003487:Cyanoacrylates / Q000494:pharmacology; D004548:Elasticity; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D006470:Hemorrhage / Q000209:etiology / Q000517:prevention & control*; D006488:Hemostasis, Surgical / Q000379:methods*; D006493:Heparin / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011409:Propylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D013536:Suture Techniques / Q000009:adverse effects*; D013552:Swine; D014014:Tissue Adhesives / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D011108:Polymers; D011409:Propylene Glycols; D014014:Tissue Adhesives; C012504:polypropylene glycol; D011092:Polyethylene Glycols; D006493:Heparin