Dynamic response of the transcriptome of a psychrophilic diatom, Chaetoceros neogracile, to high irradiance.
Large-scale RNA profiling revealed that high irradiance differentially regulated 577 out of 1,439 non-redundant genes of the Antarctic marine diatom Chaetoceros neogracile, represented on a custom cDNA chip, during 6 h of treatment. Among genes that were up- or down-regulated more than twofold within 30 min of treatment (310/1,439), about half displayed an acclimatory response during 6 h under high light. Expression of the remaining non-acclimatory genes also rapidly returned to initial levels within 30 min following a shift to low irradiance. High light altered expression of most of the photosynthesis genes (48/70), in contrast to genes in other functional categories. In addition, opposite response patterns were provoked in genes encoding fucoxanthin chlorophyll a/c binding protein (FCP), the main component of the diatom light-harvesting complex; high irradiance caused a decrease in expression of most FCP genes, but drove the rapid and specific up-regulation of ten others. C. neogracile responded very promptly to a change in light intensity by rapidly adjusting the transcript levels of FCP genes up-regulated by high light, and these dynamic adjustments coincided well with diatoxanthin (Dtx) levels formed by the xanthophyll cycle under the same conditions. The observation that the non-photochemical quenching (NPQ) capacity of this polar diatom was highly dependent on Dtx, which could bind to FCP and trigger NPQ, suggests that the up-regulated FCP gene products may participate in a photoprotective process as Dtx-binding proteins.
Park
Seunghye
S
Department of Life Science, College of Natural Science, Hanyang University, Seoul 133-791, Republic of Korea.
Jung
Gyeongseo
G
Hwang
Yong-sic
YS
Jin
EonSeon
E
D000064:Acclimatization / Q000235:genetics / Q000528:radiation effects; D017377:Diatoms / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D013997:Time Factors; D024341:Xanthophylls / Q000378:metabolism
D045342:Light-Harvesting Protein Complexes; D024341:Xanthophylls; C088752:fucoxanthin-, chlorophyll a-c-containing protein; C033808:diadinoxanthin; C103105:diatoxanthin