title: Sex- and tissue-specific expression of P450 aromatase (cyp19a1a) in the yellowtail clownfish, Amphiprion clarkii.
abstract: To investigate the role of estrogen in the gonad of yellowtail clownfish Amphiprion clarkii, we isolated cDNA encoding cytochrome P450 aromatase (Cyp19a1a) from the adult ovary. The full-length cDNA of clownfish cyp19a1a is 1928-bp long and encodes 520 amino acids. Real-time quantitative RT-PCR analysis showed that cyp19a1a was expressed mainly in the ovary of female-phase fish. In situ hybridization and immunohistochemical observations showed that positive signals were restricted to the ovarian follicle of the female-phase fish. In contrast, Cyp19a1a signal was not detected in the ambisexual gonad of the male-phase fish. These findings suggest that Cyp19a1a is involved in oogenesis in the female-phase fish, but not in the ambisexual gonad of male-phase fish.
authors.affiliation: Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa 905-0227, Japan. yasu_cob@amber.plala.or.jp
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Horiguchi
authors.forename: Saori
authors.initials: S
authors.lastname: Miura
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001141:Aromatase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004958:Estradiol / Q000097:blood; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006066:Gonads / Q000201:enzymology / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000201:enzymology / Q000378:metabolism; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012737:Sex Factors
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D004958:Estradiol; D001141:Aromatase