Role of the Y-family DNA polymerases YqjH and YqjW in protecting sporulating Bacillus subtilis cells from DNA damage.
The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillus subtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylhydroperoxide (t-BHP), mitomycin-C (M-C), and UV-C radiation. Moreover, these DNA-damaging agents increased the mutation frequency of wild-type sporulating cells to 4-azaleucine, but the production of mutants was YqjH- and YqjW-dependent. In conclusion, the results presented here indicate that YqjH/YqjW-dependent-translesion synthesis (TLS) operates in sporulating B. subtilis cells and contributes in processing spontaneous and artificially induced genetic damage, which is apparently required for an efficient sporulation process.
Rivas-Castillo
Andrea M
AM
Division of Natural and Exact Sciences, Department of Biology, University of Guanajuato, Noria Alta S/N, P.O. Box. 187, Guanajuato, 36050, Gto, México.
Yasbin
Ronald E
RE
Robleto
E
E
Nicholson
Wayne L
WL
Pedraza-Reyes
Mario
M
D001412:Bacillus subtilis / Q000201:enzymology / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000633:toxicity; D050296:Microbial Viability; D016685:Mitomycin / Q000633:toxicity; D013171:Spores, Bacterial / Q000201:enzymology / Q000502:physiology*; D014466:Ultraviolet Rays; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D016685:Mitomycin; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D004259:DNA-Directed DNA Polymerase