title: In vitro comparison of platelet storage in plasma and in four platelet additive solutions, and the effect of pathogen reduction: a proposal for an in vitro rating system.
abstract: BACKGROUND
  The introduction of platelet (PLT) additive solutions (PASs) and pathogen reduction (PR) technologies possibly allow extension of PLT shelf life. It was our aim to compare in vitro quality of leucocyte-reduced PLT concentrates (PCs) stored in various PASs, including PR, with those in plasma during 8 days of storage. The study was performed in four blood centres where each tested four conditions.
  
  
  METHODS
  In paired experiments (n = 12), buffy coat pools were made to which various storage media were added. Plasma served as reference; two centres used InterSol followed by PR (InterSol+PR) and InterSol without PR; T-sol, SSP+ and Composol were also studied.
  
  
  RESULTS
  All PCs fulfilled release criteria (pH(37 degrees C)>6.6; swirl present) until Day 8. Marked differences were seen for other parameters, including CD62P expression: 28 +/- 5; 31 +/- 7; and 39 +/- 9% for T-sol, Intersol+PR and without PR, respectively, which were higher as found for Composol (12 +/- 3%), SSP+ (15 +/- 5%) and plasma (15 +/- 6%). Three parameters (CD62P, Annexin A5, and lactate concentration) were collapsed into one rating value (6 = good quality, 0 = poor quality); PLTs in plasma had a rating of 2.8 +/- 1.0, which was higher as for T-Sol (1.5 +/- 0.5), InterSol+PR (1.3 +/- 0.6) and without PR (1.7 +/- 0.5). PLTs in potassium- and magnesium-containing PASs showed higher ratings as plasma, 4.3 +/- 0.5 for Composol and 3.8 +/- 0.8 for SSP+.
  
  
  CONCLUSIONS
  PLT concentrates in plasma, SSP+ and Composol scored better using an arbitrary rating system as PLTs stored in T-Sol or InterSol; PR further impaired rating parameters. The applicability of these differences in rating for clinical effects needs a clinical study.
authors.affiliation: Sanquin Blood Bank North West Region, Amsterdam, The Netherlands. p.vandermeer@sanquin.nl
authors.forename: P F
authors.initials: PF
authors.lastname: van der Meer
authors.forename: J-L
authors.initials: JL
authors.lastname: Kerkhoffs
authors.forename: J
authors.initials: J
authors.lastname: Curvers
authors.forename: J
authors.initials: J
authors.lastname: Scharenberg
authors.forename: D
authors.initials: D
authors.lastname: de Korte
authors.forename: A
authors.initials: A
authors.lastname: Brand
authors.forename: J
authors.initials: J
authors.lastname: de Wildt-Eggen
mesh_terms: D001424:Bacterial Infections / Q000517:prevention & control / Q000635:transmission; D001792:Blood Platelets; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D017713:Platelet Transfusion / Q000009:adverse effects / Q000379:methods; D053657:Platelet-Rich Plasma