Intratumoral IL-7 delivery by mesenchymal stromal cells potentiates IFNgamma-transduced tumor cell immunotherapy of experimental glioma.
The present study reports regression of pre-established experimental rat gliomas as a result of combining peripheral immunization using interferon gamma (IFNgamma) transduced autologous tumor cells with local intratumoral delivery of interleukin 7 (IL-7) by mesenchymal stromal cells. IL-7 alone significantly decreased the tumor area and this effect was enhanced with IFNgamma immunization. A higher density of intratumoral T-cells was observed in animals receiving combined therapies compared to rats receiving either cytokine alone suggesting that the therapeutic effect is dependent on a T-cell response.
Gunnarsson
Salina
S
The Rausing Laboratory, BMC D14, Division of Neurosurgery, Department of Clinical Sciences, Lund University, SE-221 84 Lund, Sweden. salina.gunnarsson@med.lu.se
Bexell
Daniel
D
Svensson
Andreas
A
Siesjö
Peter
P
Darabi
Anna
A
Bengzon
Johan
J
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D005910:Glioma / Q000628:therapy*; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000008:administration & dosage*; D015851:Interleukin-7 / Q000008:administration & dosage*; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects; D008297:Male; D008648:Mesoderm / Q000637:transplantation; D009374:Neoplasms, Experimental / Q000628:therapy; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000637:transplantation*; D013601:T-Lymphocytes / Q000276:immunology; D014161:Transduction, Genetic
D000970:Antineoplastic Agents; D015851:Interleukin-7; D007371:Interferon-gamma