Trametes sp. as a source of biopolymer cross-linking agents: laccase induced gelation of ferulated arabinoxylans.
This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 degrees C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 microg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase.
Castillo
Alva
A
Facultad de Zootecnia, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua 31120, Mexico.
Rascón-Chu
Agustín
A
Vargas
Georgina
G
Carvajal-Millán
Elizabeth
E
Valenzuela-Soto
Elisa
E
Sotelo-Mundo
Rogerio R
RR
Martínez
Ana Luisa
AL
D001704:Biopolymers / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology*; D014990:Xylans / Q000737:chemistry*
D001704:Biopolymers; D003432:Cross-Linking Reagents; D014990:Xylans; C085118:arabinoxylan; D042845:Laccase