Differentiation of human adipose-derived stem cells induced  by recombinantly expressed fibroblast growth factor  10 in vitro and in vivo.
The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo coculture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology.
Zhang
Xunyi
X
Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, Shanghai 200062, China. zxymeng@hotmail.com
Wu
Minjuan
M
Zhang
Weiwei
W
Shen
Jiangfan
J
Liu
Houqi
H
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000494:pharmacology*; D006801:Humans; D007267:Injections; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D051526:Fibroblast Growth Factor 10; D011994:Recombinant Proteins; D014157:Transcription Factors