Augmented humoral and cellular immune response of hepatitis B virus DNA vaccine by micro-needle vaccination using Flt3L as an adjuvant.
The purpose of this work was to assess the immune response against plasmid DNA encoding hepatitis B virus (HBV) HBsAg in C57BL/6 mice inoculated by micro-needle. Fms-like tyrosine kinase 3 ligand (Flt3L), a hematopoietic growth factor, was used as an adjuvant. Immune response was determined by analysis of cytokine production. In addition, cytotoxic lymphocyte (CTL) activity was measured 7 days after in vitro addition of tumor cells (CT26/S) stabling expressing HBsAg. The efficacy of immunoprotection against CT26/S was determined by antibody response to this challenge. A strong antibody response was induced by inoculation with the DNA vaccine via micro-needles assisted with Flt3L, Administration of the vaccine to splenocytes induced significantly higher levels of interleukin-12 and gamma interferon. CTL response to the vaccine was stronger than that stimulated by intramuscular or micro-needle injections alone. Our study suggests useful strategies for improving the efficacy of vaccines against HBV and other pathogens.
Zhou
Qi
Q
Department of Medical Genetics, Second Military Medical University, 800 Xiang-Yin Road, Shanghai 200433, PR China.
Wang
Fang
F
Yang
Fu
F
Wang
Yue
Y
Zhang
Xiaoying
X
Sun
Shuhan
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D006510:Hepatitis B Antibodies / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination / Q000295:instrumentation* / Q000379:methods*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D008565:Membrane Proteins; D019444:Vaccines, DNA; C084881:flt3 ligand protein; D018664:Interleukin-12; D007371:Interferon-gamma