title: Improved method for identifying and quantifying olive oil phenolic compounds and their metabolites in human plasma by microelution solid-phase extraction plate and liquid chromatography-tandem mass spectrometry.
abstract: Two methods based on solid-phase extraction (SPE) using traditional cartridges and microelution SPE plates (muSPE) as the sample pre-treatment, and an improved liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and compared to determine the phenolic compounds in virgin oil olive from plasma samples. The phenolic compounds studied were hydroxytyrosol, tyrosol, homovanillic acid, p-coumaric acid, 3,4-DHPEA-EDA, p-HPEA-EDA, luteolin, apigenin, pinoresinol and acetoxypinoresinol. Good recoveries were obtained in both methods, and the LOQs and LODs were similar, in the range of low muM. The advantage of muSPE, in comparison with SPE cartridges, was the lack of the evaporation step to pre-concentrate the analytes. The muSPE-UPLC-ESI-MS/MS method developed was then applied to determine the phenolic compounds and their metabolites, in glucuronide, sulphate and methylated forms, in human plasma after the ingestion of virgin olive oil.
authors.affiliation: Food Technology Department, XaRTA-TPV, Escuela Técnica Superior de Ingeniería Agraria, University of Lleida, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Suárez
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Romero
authors.forename: Alba
authors.initials: A
authors.lastname: Macià
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Valls
authors.forename: Sara
authors.initials: S
authors.lastname: Fernández
authors.forename: Rosa
authors.initials: R
authors.lastname: Solà
authors.forename: Maria-José
authors.initials: MJ
authors.lastname: Motilva
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis* / Q000378:metabolism*; D010938:Plant Oils / Q000032:analysis* / Q000378:metabolism; D010949:Plasma / Q000737:chemistry* / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils