title: A nanobiosensor to detect single hybridization events.
abstract: An economical nanoarray method to electrically detect hybridization events is demonstrated. As a proof of concept, we fabricated a sensor for DNA sequencing, in which targets are oligonucleotides conjugated to gold nanoparticles. As a consequence of target-probe binding events, a conductive bridge forms between two electrodes, resulting in a quantized change in conductivity. This enables a robust detection of a few (down to single) hybridization events and can be potentially applied also to other binding events (like specific interactions between proteins, antibodies, ligands and receptors). Moreover, target amplification techniques (such as PCR) are no longer necessary.
authors.affiliation: National Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, Università del Salento, IIT-Research Unit, v. Arnesano 16, I-73100 Lecce, Italy. giuseppe.maruccio@unisalento.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.forename: Pasquale
authors.initials: P
authors.lastname: Marzo
authors.forename: Valentina
authors.initials: V
authors.lastname: Arima
authors.forename: Antonio
authors.initials: A
authors.lastname: Della Torre
authors.forename: Ross
authors.initials: R
authors.lastname: Rinaldi
authors.forename: Teresa
authors.initials: T
authors.lastname: Pellegrino
authors.forename: Roman
authors.initials: R
authors.lastname: Krahne
authors.forename: Roberto
authors.initials: R
authors.lastname: Cingolani
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D006046:Gold; D053768:Metal Nanoparticles; D036103:Nanotechnology / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D006046:Gold; D004247:DNA