Effects of external irrigation on mitomycin-C concentration in rabbit aqueous and vitreous humor.
OBJECTIVE
These experiments were performed to determine what effect external irrigation with balanced salt has on limiting penetration of mitomycin-C into the aqueous and vitreous.


METHODS
Bilateral 5 min scleral applications of mitomycin-C (0.5 mg/ml) were performed in 21 rabbits using identical 6x4x1 mm cellulose sponges uniformly soaked with 0.2 ml of the mitomycin-C solution. Irrigation of one eye, randomly selected, was then carried out with 10 ml of balanced salt solution over 1 min. High performance liquid chromatography was used to analyse aqueous and vitreous samples obtained from separate animals at 5, 15, 30, and 60 min and at 2,4, and 6 h after sponge removal.


RESULTS
Nonirrigated eyes demonstrated continual rise in aqueous mitomycin-C concentration over 1 h. Irrigated eyes demonstrated lower mitomycin-C concentrations at all times studied and a continual fall in aqueous concentration after 15 min. Vitreous mitomycin-C was detected in both groups only at 5 min.


CONCLUSIONS
Irrigation with balanced salt substantially reduces intraocular diffusion of mitomycin-C.
Prata
J A
JA
*Department of Ophthalmology, University of Southern California School of Medicine and the Doheny Eye Institute, Los Angeles, California, U.S.A.; daggerEscola Paulista de Medicina, Department of Ophthalmology, Saõ Paulo, Brazil; and double daggerUniversity of Southern California School of Pharmacy, Los Angeles, California, U.S.A.
Minckler
D S
DS
Koda
R T
RT