title: Time-dependence and visualization of TiO2 and Pt particle biodistribution in mice.
abstract: Micro- and nano-sized materials have received much attention regarding their biocompatibility and toxicity. To understand the influence of such materials on animals, it is very important to determine their internal distribution behavior. In this study, the biodistributions of Pt and TiO2 micro- and nano-sized particles in mice were estimated and visualized by X-ray scanning analytical microscopy and inductively coupled plasma-atomic emission spectroscopy. We also determined the effect of particle size, difference between metal and oxide, and time dependence for the distributions, because the biodistribution depends upon both the chemical character of materials and the size of particles. The results of the present study indicated that the difference in chemical character had a greater effect than did particle size. We predict that X-ray scanning analytical microscopy will be a useful method for studying biodistribution of micro- and nano-sized particles, because this method requires no labeling or treatment of the target particles.
authors.affiliation: Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University, North 13, West 7, Kita-ku, Sapporo 060-8586, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Abe
authors.forename: Chika
authors.initials: C
authors.lastname: Koyama
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Akasaka
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kuboki
authors.forename: Fumio
authors.initials: F
authors.lastname: Watari
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008853:Microscopy / Q000379:methods; D053758:Nanoparticles; D010984:Platinum / Q000493:pharmacokinetics*; D013057:Spectrum Analysis / Q000379:methods; D014025:Titanium / Q000493:pharmacokinetics*; D014965:X-Rays
chemical_list: C009495:titanium dioxide; D010984:Platinum; D014025:Titanium