title: Breadth of human immunodeficiency virus-specific neutralizing activity in sera: clustering analysis and association with clinical variables.
abstract: Induction of antibodies that neutralize a broad range of human immunodeficiency virus type 1 (HIV-1) isolates is a major goal of vaccine development. To study natural examples of broad neutralization, we analyzed sera from 103 HIV-1-infected subjects. Among progressor patients, 20% of sera neutralized more than 75% of a panel of 20 diverse viral isolates. Little activity was observed in sera from long-term nonprogressors (elite controllers). Breadth of neutralization was correlated with viral load, but not with CD4 count, history of past antiretroviral use, age, gender, race/ethnicity, or route of exposure. Clustering analysis of sera by a novel method identified a statistically robust subgrouping of sera that demonstrated broad and potent neutralization activity.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Doria-Rose
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Klein
authors.forename: Marcus G
authors.initials: MG
authors.lastname: Daniels
authors.forename: Sijy
authors.initials: S
authors.lastname: O'Dell
authors.forename: Martha
authors.initials: M
authors.lastname: Nason
authors.forename: Alan
authors.initials: A
authors.lastname: Lapedes
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Bhattacharya
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Migueles
authors.forename: Richard T
authors.initials: RT
authors.lastname: Wyatt
authors.forename: Bette T
authors.initials: BT
authors.lastname: Korber
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.forename: Mark
authors.initials: M
authors.lastname: Connors
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood* / Q000276:immunology; D016000:Cluster Analysis; D015658:HIV Infections / Q000276:immunology / Q000821:virology; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000276:immunology*; D006801:Humans
chemical_list: D057134:Antibodies, Neutralizing