title: Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas.
abstract: OBJECTIVE
  To investigate the expression of aquaporin (AQP) in eutopic and ectopic endometrial tissues from women with endometriomas.
  
  
  METHODS
  Controlled laboratory research.
  
  
  METHODS
  Hospital-based unit for gynecology and obstetrics and research laboratories.
  
  
  METHODS
  Premenopausal women undergoing laparoscopy for endometriomas.
  
  
  METHODS
  Endometrial biopsy samples obtained from 70 women with endometriomas.
  
  
  METHODS
  Semiquantitative analysis by immunohistochemistry.
  
  
  RESULTS
  Aquaporins 2, 5, and 8 were mainly located in luminal and glandular epithelia. The frequency of positive immunostaining for aquaporins 2, 5, and 8 decreased in ectopic compared with eutopic endometria. Aquaporins 2, 5, and 8 were found at a low frequency in the endometria in early proliferative phases but at a higher frequency in late proliferative and secretory phases. There were no significant differences in the menstrual cycle of the proliferative phase and secretory phase in the two groups.
  
  
  CONCLUSIONS
  Aquaporins 2, 5, and 8 were expressed with greater frequency in eutopic endometrial cells than inectopic endometrial cells, suggesting that eutopic endometrial cells have stronger migration activity than ectopic endometrial cells in women with endometriosis.
authors.affiliation: Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Xiu-Xiu
authors.initials: XX
authors.lastname: Jiang
authors.affiliation: Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Riu-Jin
authors.initials: RJ
authors.lastname: Wu
authors.affiliation: Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Kai-Hong
authors.initials: KH
authors.lastname: Xu
authors.affiliation: Department of Pathology, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Zhou
authors.affiliation: Department of Laboratory, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Department of Laboratory, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Sun
authors.affiliation: Department of Gynecology and Obstetrics, Women's Hospital, Zhejiang University Medical College, Zhejiang Province, Hangzhou City, People's Republic of China. Electronic address: janet418@sina.com.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
mesh_terms: D000328:Adult; D020346:Aquaporins / Q000378:metabolism*; D002828:Choristoma / Q000378:metabolism* / Q000473:pathology; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: D020346:Aquaporins