[Arteriogenesis induced by intramyocardial recombinant adeno-associated virus vector encoding human CD151 cDNA gene transfer in swines with coronary artery occlusion].
OBJECTIVE
To investigate the efficacy of CD151 gene delivery in promoting blood perfusion in swines after myocardial infarction.


METHODS
Swines received coronary artery ligation and intramyocardial injection with rAAV-CD151, rAAV-anti-CD151 or rAAV-GFP. Eight weeks after vector injection, Western blot, immunostaining and 13N-labeled NH3 PET were performed to detect gene expression and biological effects of various treatments.


RESULTS
High level of CD151 protein expression was detected in the rAAV-CD151 group. The capillary density in the rAAV-CD151 group [(83.8 +/- 6.7) n/mm2] was significantly higher than that in the control group [(33.2 +/- 4.5) n/mm2] and rAAV-GFP group [(41.6 +/- 5.6) n/mm2] (all P<0.05); the arteriole density in the rAAV-CD151 group [(16.4 +/- 2.5) n/mm2] was also higher than that in the control group [(6.6 +/- 2.3) n/mm2] and the rAAV-GFP group [(8.4 +/- 1.6) n/mm2] (all P<0.05). However, the lowest capillary density and arteriole density were evidenced in rAAV-anti-CD151 group. Myocardial blood perfusion was significantly increased in rAAV-CD151 group and significantly reduced in rAAV-anti-CD151 group (all P<0.05 vs. control).


CONCLUSIONS
Intramyocardial injection of rAAV-CD151 could enhance the myocardial express of CD151 protein, increase capillary and arteriole densities and improve blood perfusion in swine with myocardial infarction.
Zuo
Hou-juan
HJ
Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Zheng-xiang
ZX
Liu
Xiao-chun
XC
Zeng
He-song
HS
Wen
Sha
S
Liu
Tao
T
Wang
Dao-wen
DW
Zhang
Xin
X
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D054059:Coronary Occlusion / Q000628:therapy*; D000229:Dependovirus / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000628:therapy*; D018919:Neovascularization, Physiologic; D013552:Swine; D013556:Swine, Miniature; D060185:Tetraspanin 24; D016896:Treatment Outcome
D015703:Antigens, CD; C554715:CD151 protein, human; D060185:Tetraspanin 24