title: Cytotoxic effect of atrazine on murine  B-lymphocytes in vitro.
abstract: When a stable cell line from mouse connective tissue was used to test the cytotoxicity of atrazine no significant influence on cell proliferation was observed at concentrations of 0.003-200 microM. However, freshly isolated mouse spleen cells reacted very sensitively to the presence of atrazine. During the course of the experiment the spleen cells were pretreated with atrazine (0.05-5 microM) before the proliferation of B-lymphocytes was stimulated with LPS. A reduced number of developing B-blasts was observed in the atrazine pretreated cultures. The B-blasts were fused with myeloma cells and cultivated in HAT (hypoxantine, aminopterin,thymidine)-medium for hybridoma selection. The number of surviving clones was significantly lower on atrazine pretreatment.
authors.affiliation: GBF, Geseilschaft fur Biotechnologische Forschung mbH, Mascheroder Weg 1, DW-3300 Braunschweig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Böcher
authors.forename: T
authors.initials: T
authors.lastname: Böldicke
authors.forename: F
authors.initials: F
authors.lastname: Sasse
mesh_terms: D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002459:Cell Fusion; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D006540:Herbicides / Q000633:toxicity*; D006825:Hybridomas; D007739:L Cells; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D003603:Cytotoxins; D006540:Herbicides; D001280:Atrazine