title: Expression of matrix metalloproteinase-9 and significance of a macrophage assay in eosinophilic granuloma.
abstract: Although most cases of eosinophilic granuloma (an immune-mediated lesion) do not behave in an aggressive manner, occasionally, recurrence after treatment has been observed. This study aims to investigate the significance and relationship of matrix metalloproteinase-9 (MMP-9) and infiltration of macrophages in eosinophilic granulomas (EGs). The immunohistochemical strept avidin-biotin complex method was used to detect the expression of MMP-9 and CD-68 (for labeling macrophages) in 13 cases of EG. Three of whom showed local recurrence. The results showed that many of the Langerhans histiocytic cells, which reacted positively with MMP-9 protein antibody, were immunolabeled with CD-68 antibody. The results showed that there is a highly significant relationship between the expression of MMP-9 and macrophage count in EGs (P < .001), with recurrent lesions showing a higher mean expression of both MMP-9 and CD-68. In conclusion, there may be a cooperation between macrophages and MMP-9 in EGs, which correlates well with local recurrence. Their expression may therefore provide some prognostic indication of the possible aggressive and recurrence behavior of this lesion.
authors.affiliation: Faculty of Dentistry, Oral Pathology Department, Mansoura University, 35516 Mansoura, Egypt. zyadam@yahoo.com
authors.forename: Manal Mohamed
authors.initials: MM
authors.lastname: Zyada
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D002452:Cell Count; D004803:Eosinophilic Granuloma / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D020780:Matrix Metalloproteinase 9; C067980:CD68 antigen, human; D000097382:CD68 Molecule