[New approaches to the real-time detection of nucleotide mismatches by means of chimeric hybridization probes].
New approaches to the detection of impaired nucleotides based on the allele specific ligation of a "C probe" followed by rolling circle amplification have been developed. The detection of amplification products was realized by using enzymatic and deoxyribozyme digestion of fluorescently-labeled DNA-RNA-DNA chimeric oligonucleotide structures in cycling probe technology (CPT) in real-time mode.
Garafutdinov
R R
RR
Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, Russia. garafutdinovr@mail.ru
Nikonorov
Iu M
IuM
Chemeris
D A
DA
Postrigan'
B N
BN
Chubukova
O V
OV
Talipov
R F
RF
Vakhitov
V A
VA
Chemeris
A V
AV
D020137:Base Pair Mismatch; D005456:Fluorescent Dyes / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes