title: [Proteomic analysis of mitochondria from the Bos taurus heart. II. Identification of mitochondrial soluble proteins].
abstract: A fraction of the so-called mitochondrial soluble proteins was obtained after the destruction of purified mitochondria by sonication according to the previously found approach to the identification of protein subsets of the Bos taurus heart proteome. A tryptic destruction of these proteins was achieved. Approximately half of the tryptic hydrolysate was separated into two fractions of cysteine-containing and cysteine-free peptides by covalent chromatography on Thiopropyl Sepharose 4B. The cysteine-containing peptides were modified by iodoacetamide. The peptides were mass-spectrometrically identified in all the three fractions of tryptic hydrolysate, and the proteins were searched for in the amino acid sequence databases. There were 213 unique proteins reliably identified.
authors.forename: E V
authors.initials: EV
authors.lastname: Daĭnichenko
authors.forename: A N
authors.initials: AN
authors.lastname: Boldyrev
authors.forename: K V
authors.initials: KV
authors.lastname: Baryliuk
authors.forename: N B
authors.initials: NB
authors.lastname: Poliakov
authors.forename: V A
authors.initials: VA
authors.lastname: Grinkevich
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D030562:Databases, Protein; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008929:Mitochondria, Heart / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012995:Solubility; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D024101:Mitochondrial Proteins