title: Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake.
abstract: Four new fluorescent derivatives of cholic acid have been synthesized; they incorporate a dansyl moiety at 3alpha-, 3beta-, 7alpha- or 7beta- positions. These cholic acid analogs are UV photoactive and also exhibit green fluorescence. In addition, they have been demonstrated to be suitable for studying the kinetics of bile acid transport by flow cytometry.
authors.affiliation: Instituto de Tecnología Química-Departamento de Química (UPV-CSIC), Avda de los Naranjos s/n, E-46022, Valencia, Spain.
authors.forename: Jana
authors.initials: J
authors.lastname: Rohacova
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Marin
authors.forename: Alicia
authors.initials: A
authors.lastname: Martínez-Romero
authors.forename: José-Enrique
authors.initials: JE
authors.lastname: O'Connor
authors.forename: M Jose
authors.initials: MJ
authors.lastname: Gomez-Lechon
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Donato
authors.forename: Jose V
authors.initials: JV
authors.lastname: Castell
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
mesh_terms: D000818:Animals; D002793:Cholic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D003619:Dansyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D005453:Fluorescence; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008968:Molecular Conformation; D010777:Photochemistry; D051381:Rats; D013237:Stereoisomerism; D014466:Ultraviolet Rays
chemical_list: D002793:Cholic Acids; D003619:Dansyl Compounds