title: Triterpenes from Warszewiczia coccinea (Rubiaceae) as inhibitors of acetylcholinesterase.
abstract: Seventy-six plant extracts from the Panamanian flora have been screened for acetylcholinesterase (AChE) inhibitors by thin-layer chromatography (TLC) bioautography. The most promising extracts with AChE inhibitory and free radical scavenging activities at 100 microg were those of Tabernaemontana panamensis (Markgr., Boiteau & L. Allorge) Leeuwenb., Pentagonia macrophylla Benth., and Warszewiczia coccinea (Vahl) Klotzsch. Bioguided fractionation of W. coccinea stem extract afforded two triterpenes, 3beta,6beta,19alpha-trihydroxy-urs-12-en-28-oic acid (1) and 3beta,6beta-dihydroxy-olean-12-en-28-oic acid (sumaresinolic acid) (2), with AChE inhibitory activity. Their structures were determined by spectroscopic methods. This is the first report of these bioactive triterpenes in W. coccinea.
authors.affiliation: Department of Pharmacal Sciences, Harrison School of Pharmacy, Auburn University, 4306B Walker Building, Auburn, AL 36849, USA.
authors.forename: Angela I
authors.initials: AI
authors.lastname: Calderón
authors.forename: Johayra
authors.initials: J
authors.lastname: Simithy
authors.forename: Giuliana
authors.initials: G
authors.lastname: Quaggio
authors.forename: Alex
authors.initials: A
authors.lastname: Espinosa
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: López-Pérez
authors.forename: Mahabir P
authors.initials: MP
authors.lastname: Gupta
mesh_terms: D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D019666:Rubiaceae / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D014315:Triterpenes; D009828:Oleanolic Acid; C545911:3beta,6beta-dihydroxyolean-12-en-28-oic acid