Clostridium arbusti sp. nov., an anaerobic bacterium isolated from pear orchard soil.
An obligately anaerobic, Gram-positive, spore-forming bacterial strain, designated SL206(T), was isolated from pear orchard soils. Strain SL206(T) cells were straight or slightly curved rods, with motility by peritrichate flagella. Cell walls contained meso-diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. The major fatty acids were C(16 : 0), C(18 : 1)omega9c and summed feature 10 (containing C(18 : 1)omega11c/9t/6t). API 20A reactions were negative for oxidase, catalase and acid production from l-rhamnose, sucrose, trehalose, d-xylose, melezitose, salicin and d-sorbitol, and positive for acid production from d-glucose, sucrose, maltose, d-mannose and raffinose. Glucose was fermented to acetate, butyrate, CO(2), H(2) and ethanol in culture. The G+C content of the genomic DNA was 31.1 mol%. Based on comparative 16S rRNA gene sequence analysis, the isolate belonged to the genus Clostridium and formed a clade with Clostridium pasteurianum. The species most closely related to strain SL206(T) were C. pasteurianum (98.6 % similarity) and Clostridium acidisoli (97.8 % similarity). In DNA-DNA relatedness studies, the isolate had 59.5 % relatedness with C. pasteurianum and thus represented a unique species. On the basis of these studies, strain SL206(T) (=KCTC 5449(T) =JCM 14858(T)) is proposed to represent the type strain of a novel species, Clostridium arbusti sp. nov.
Jung
Min Young
MY
Department of Microbiology, College of Medicine, Chung-Ang University, 221 Heukseok-dong, Dongjak-ku, Seoul 156-756, Republic of Korea.|Korean Collection for Type Cultures, Biological Resource Center, KRIBB, 52 Oeundong, Daejeon 305-806, Republic of Korea.
Park
In-Soon
IS
Korean Collection for Type Cultures, Biological Resource Center, KRIBB, 52 Oeundong, Daejeon 305-806, Republic of Korea.
Kim
Wonyong
W
Department of Microbiology, College of Medicine, Chung-Ang University, 221 Heukseok-dong, Dongjak-ku, Seoul 156-756, Republic of Korea.
Kim
Hong Lim
HL
Namhae Sub-Station, National Horticultural Research Institute, RDA, Namhae, 668-812, Republic of Korea.
Paek
Woon Kee
WK
National Science Museum, 32-2 Guseong-dong, Yuseong-gu, Daejeon 305-705, Republic of Korea.
Chang
Young-Hyo
YH
Korean Collection for Type Cultures, Biological Resource Center, KRIBB, 52 Oeundong, Daejeon 305-806, Republic of Korea.
D000693:Anaerobiosis; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D031989:Pyrus / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S