title: Acute oral safety study of dairy fat rich in trans-10 C18:1 versus vaccenic plus conjugated linoleic acid in rats.
abstract: The acute oral toxicity of a trans-10 C18:1-rich milk fat (T10, 20% of total FA), and a trans-11 C18:1+cis-9 trans-11 C18:2-rich milk fat (T11-CLA, 14% and 4.8% of total FA, respectively) was studied in rats receiving a single oral dose of 2000 mg/kg body weight (BW). T10 and T11-CLA milk fats were well tolerated; no adverse effects or mortality were observed during the 2-week observation period. Two weeks following a single oral dose of 2000 mg/kg BW of T10 and T11-CLA milk fats there were no changes in haematological and serum chemistry parameters (excepting plasma lipid) organ weights, gross pathology or histopathology. In rats treated with T10 milk fat a significant increase of triglycerides was observed. In contrast, in rats treated with T11-CLA milk fat significantly decreased triglycerides were detected. It was concluded that dairy fats rich in T10 and T11-CLA have a low order of acute toxicity, the oral lethal dose (DL50) for male and female rats are in excess of 2000 mg/kg BW. Our results suggest that the T10 milk fat treatment tended to increase triglycerides concentrations, whereas the T11-CLA milk fat treatment tended to reduce it.
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, E-28040 Madrid, Spain. anadon@vet.ucm.es
authors.forename: Arturo
authors.initials: A
authors.lastname: Anadón
authors.forename: María R
authors.initials: MR
authors.lastname: Martínez-Larrañaga
authors.forename: María A
authors.initials: MA
authors.lastname: Martínez
authors.forename: Irma
authors.initials: I
authors.lastname: Ares
authors.forename: Eva
authors.initials: E
authors.lastname: Ramos
authors.forename: Pilar
authors.initials: P
authors.lastname: Gómez-Cortés
authors.forename: Manuela
authors.initials: M
authors.lastname: Juárez
authors.forename: Miguel A
authors.initials: MA
authors.lastname: De la Fuente
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002624:Chemistry, Clinical; D019587:Dietary Supplements; D004435:Eating / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005503:Food Additives / Q000633:toxicity*; D006403:Hematologic Tests; D044243:Linoleic Acids, Conjugated / Q000633:toxicity*; D008136:Longevity / Q000187:drug effects; D008297:Male; D008892:Milk / Q000737:chemistry*; D009829:Oleic Acids / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012756:Sheep; D023382:Toxicity Tests, Acute
chemical_list: D005227:Fatty Acids; D005503:Food Additives; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; C496197:trans-10,cis-12-conjugated linoleic acid; C065593:cis-vaccenic acid