title: Removal of species constraints in antibody detection.
abstract: Serum antibodies from myriad species, particularly birds, can provide key information regarding the transmission and the expansion of the territory of emerging pathogens. Expedient antibody analysis is constrained by a lack of species-specific reagents, a deficiency potentially highlighted by the recent swine-origin influenza A virus (H1N1) outbreak. Available methodologies present difficulties that discourage thorough serologic monitoring of potential disease vectors or hosts. Rapid high-throughput procedures that combined serum amine labeling via biotinylation, contaminant removal, and microsphere-based immunoassays for antibodies to three arboviruses were developed. Agent-specific adaptations of this simple format should facilitate expanded surveillance and diagnostic capabilities regarding pathogens of human and veterinary importance.
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd., Fort Collins, CO 80521, USA. ajj1@cdc.gov
authors.forename: Alison Jane
authors.initials: AJ
authors.lastname: Basile
authors.forename: Brad J
authors.initials: BJ
authors.lastname: Biggerstaff
authors.forename: Olga L
authors.initials: OL
authors.lastname: Kosoy
authors.forename: Shilpa R
authors.initials: SR
authors.lastname: Junna
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Panella
authors.forename: Ann M
authors.initials: AM
authors.lastname: Powers
authors.forename: Lillian M
authors.initials: LM
authors.lastname: Stark
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Nemeth
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology*; D004665:Encephalitis Virus, St. Louis / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral