Detection of allergen walnut component in food by an improved real-time PCR method.
A real-time PCR method aimed at the gene sequence of the walnut vicilin-like seed storage protein was established for the detection of the allergen walnut in food. The primers and probe were designed based on published methods. The method provided positive results for walnut and negative results for other tested agricultural plant materials including pecan. The intrinsic detection limit of the method was 0.00125 ng of walnut DNA, and the practical detection limit was 0.001% (wt/wt) walnut content in wheat; both of these values are lower than that of previously published methods. Therefore, this real-time PCR method is sufficiently specific and sensitive for the detection of walnut component in food.
Wang
Haiyan
H
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Chaoyang District, Beijing 100123, People's Republic of China.
Yuan
Fei
F
Wu
Yajun
Y
Yang
Hairong
H
Xu
Baoliang
B
Liu
Zhongxue
Z
Chen
Ying
Y
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D003257:Consumer Product Safety; D017931:DNA Primers; D015342:DNA Probes; D005506:Food Contamination / Q000032:analysis*; D005512:Food Hypersensitivity; D006801:Humans; D031324:Juglans / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D055314:Seed Storage Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000485:Allergens; D017931:DNA Primers; D015342:DNA Probes; D055314:Seed Storage Proteins; C015575:vicilin protein, plant