Experiment on the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles.
The objective of this study was to investigate the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles. Ultrasound (US) combined with phospholipid-based microbubbles (MB) was used to enhance the susceptibility of colon cancer cell line SWD-620 to anticancer drugs Topotecan hydrochloride (TOP). Experiments were designed to investigate the influence of main factors on cell viability and cell inhibition, such as US intensity, MB concentration, drug combination with MB, asynchronous action between US triggered cavitation and drug entering cell, MB particle size. US exposure for 10 sec with US probe power at 0.6 W/cm(2) had satisfied cell viability. Treated with US combined with 15% MB, cell viability maintained more than 85% and cell inhibition 86.16%. Under optimal US combined with MB, TOP showed much higher cell inhibition than that of only TOP group. Cell inhibition under short delayed time (<2 h) for TOP addition did not show obvious difference. In terms of MB particle size, the order of cell inhibition was: Mixture > Micron bubble part > Nanometer bubble part. US combined with MB can enhance the susceptibility of cancer cells to chemotherapeutic drug, which may provide a potential method for US-mediated tumor chemotherapy.
Zhao
Ying-Zheng
YZ
Pharmacy School, Wenzhou Medical College, Zhejiang Province, China. lctuua@yahoo.com.cn
Gao
Hui-Sheng
HS
Zhou
Zhi-Cai
ZC
Tang
Qin-Qin
QQ
Lu
Cui-Tao
CT
Jin
Zhuo
Z
Tian
Ji-Lai
JL
Xu
Yan-Yan
YY
Tian
Xin-Qiao
XQ
Wang
Lee
L
Kong
Fan-Lei
FL
Li
Xiao-Kun
XK
Huang
Pin-Tong
PT
He
Hui-Liao
HL
Wu
Yan
Y
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D045423:Microbubbles; D010743:Phospholipids / Q000737:chemistry; D013010:Sonication; D019772:Topotecan / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D014465:Ultrasonics
D000970:Antineoplastic Agents; D010743:Phospholipids; D019772:Topotecan