title: An in vitro study of the antioxidant activities and effect on human DNA of the Chinese herbal decoction 'Liu Wei Di Huang'.
abstract: A Chinese medicinal formulation, 'Liu Wei Di Huang', and its individual components have been tested for the genoprotective effect on human DNA by the comet assay. Results showed no DNA protection contributed by this prescription. However, the aqueous extracts of individual herbs, namely Cortex Moutan and Rhizoma Dioscoreae, were able to decrease by 10-15% the DNA stand break from hydrogen peroxide-mediated oxidative stress. This provides insight to further evaluate the interaction among herbs and the search for the active ingredient responsible for the DNA protective effect.
authors.affiliation: Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology Foundation, Avenida Wai Long, Taipa, Macau. ytszeto@must.edu.mo
authors.forename: Yim-Tong
authors.initials: YT
authors.lastname: Szeto
authors.forename: Pui-Cheong
authors.initials: PC
authors.lastname: Lei
authors.forename: Kam-Leong
authors.initials: KL
authors.lastname: Ngai
authors.forename: Andy Tak-Wah
authors.initials: AT
authors.lastname: Yiu
authors.forename: Catarina Sin-Pek
authors.initials: CS
authors.lastname: Chan
authors.forename: Edith Wun-Fong
authors.initials: EW
authors.lastname: Kok
authors.forename: Chi-Wa
authors.initials: CW
authors.lastname: Leong
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D027742:Dioscorea; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D041743:Herb-Drug Interactions; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D062385:Hydroxybenzoates / Q000032:analysis; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D029596:Paeonia; D027343:Rhizome
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C042579:Liuwei Dihuang Decoction; D006861:Hydrogen Peroxide