title: Thrombospondin 1 accelerates synaptogenesis in hippocampal neurons through neuroligin 1.
abstract: In cultured rat hippocampal neurons, we found that thrombospondin 1 (TSP1) increased the speed of synapse formation in young neurons, but not the final density of synapses in mature neurons. TSP1 interacted with neuroligin 1 (NL1) and application of the NL1 extracellular domain blocked TSP1-induced synaptogenesis. Furthermore, knocking down endogenous NL1 inhibited TSP1's effect. Our results indicate that TSP1 accelerates the speed of synaptogenesis through NL1 in hippocampal neurons.
authors.affiliation: Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Junyu
authors.initials: J
authors.lastname: Xu
authors.forename: Nan
authors.initials: N
authors.lastname: Xiao
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
mesh_terms: D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015816:Cell Adhesion Molecules, Neuronal; D002478:Cells, Cultured; D000076122:Disks Large Homolog 4 Protein; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D051528:Guanylate Kinases; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000187:drug effects* / Q000502:physiology; D016704:Synapsins / Q000378:metabolism; D019700:Thrombospondin 1 / Q000235:genetics / Q000494:pharmacology*; D014162:Transfection / Q000379:methods
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D015816:Cell Adhesion Molecules, Neuronal; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D011973:Receptors, LDL; C481348:SPZ1 protein, human; D016704:Synapsins; D019700:Thrombospondin 1; C036053:VLDL receptor; C093438:neuroligin 1; D049452:Green Fluorescent Proteins; D051528:Guanylate Kinases