Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor array.
Aerobic batch cultivations of Candida utilis were carried out in two micro bioreactors with a working volume of 100 muL operated in parallel. The dimensions of the micro bioreactors were similar as the wells in a 96-well microtiter plate, to preserve compatibility with the current high-throughput cultivation systems. Each micro bioreactor was equipped with an electrochemical sensor array for the online measurement of temperature, pH, dissolved oxygen, and viable biomass concentration. Furthermore, the CO(2) production rate was obtained from the online measurement of cumulative CO(2) production during the cultivation. The online data obtained by the sensor array and the CO(2) production measurements appeared to be very reproducible for all batch cultivations performed and were highly comparable to measurement results obtained during a similar aerobic batch cultivation carried out in a conventional 4L bench-scale bioreactor. Although the sensor chip certainly needs further improvement on some points, this work clearly shows the applicability of electrochemical sensor arrays for the monitoring of parallel micro-scale fermentations, e.g. using the 96-well microtiterplate format.
van Leeuwen
Michiel
M
Dept. of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC, Delft, The Netherlands.
Krommenhoek
Erik E
EE
Heijnen
Joseph J
JJ
Gardeniers
Han
H
van der Wielen
Luuk A M
LA
van Gulik
Walter M
WM
D000332:Aerobiosis; D018533:Biomass; D019149:Bioreactors; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002175:Candida / Q000166:cytology*; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
D002245:Carbon Dioxide; D010100:Oxygen