The Chloroplast Function Database: a large-scale collection of Arabidopsis Ds/Spm- or T-DNA-tagged homozygous lines for nuclear-encoded chloroplast proteins, and their systematic phenotype analysis.
A majority of the proteins of the chloroplast are encoded by the nuclear genome, and are post-translationally targeted to the chloroplast. From databases of tagged insertion lines at international seed stock centers and our own stock, we selected 3246 Ds/Spm (dissociator/suppressor-mutator) transposon- or T-DNA-tagged Arabidopsis lines for genes encoding 1369 chloroplast proteins (about 66% of the 2090 predicted chloroplast proteins) in which insertions disrupt the protein-coding regions. We systematically observed 3-week-old seedlings grown on agar plates, identified mutants with abnormal phenotypes and collected homozygous lines with wild-type phenotypes. We also identified insertion lines for which no homozygous plants were obtained. To date, we have identified 111 lines with reproducible seedling phenotypes, 122 lines for which we could not obtain homozygotes and 1290 homozygous lines without a visible phenotype. The Chloroplast Function Database presents the molecular and phenotypic information obtained from this resource. The database provides tools for searching for mutant lines using Arabidopsis Genome Initiative (AGI) locus numbers, tagged line numbers and phenotypes, and provides rapid access to detailed information on the tagged line resources. Moreover, our collection of insertion homozygotes provides a powerful tool to accelerate the functional analysis of nuclear-encoded chloroplast proteins in Arabidopsis. The Chloroplast Function Database is freely available at http://rarge.psc.riken.jp/chloroplast/. The homozygous lines generated in this project are also available from the various Arabidopsis stock centers. We have donated the insertion homozygotes to their originating seed stock centers.
Myouga
Fumiyoshi
F
RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan.
Akiyama
Kenji
K
Motohashi
Reiko
R
Kuromori
Takashi
T
Ito
Takuya
T
Iizumi
Haruko
H
Ryusui
Rie
R
Sakurai
Tetsuya
T
Shinozaki
Kazuo
K
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D030561:Databases, Nucleic Acid; D009154:Mutation; D010641:Phenotype; D036226:Seedlings / Q000235:genetics / Q000254:growth & development
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA