title: Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat.
abstract: OBJECTIVE
  The objective of this study was to histologically analyze the influence of the platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in the calvaria of diabetic rats.
  
  
  METHODS
  A total of 20 diabetic rats were divided into 2 groups: C (control) and PRP. A 5-mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group PRP, 0.35 mL of PRP was placed in the defects. All animals were humanely killed 30 days postoperatively. Histometric and histologic analyses were performed. Data were statistically analyzed (t test, P < .05).
  
  
  RESULTS
  No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C (37.22% +/- 6.00% and 21.68% +/- 11.35%, respectively).
  
  
  CONCLUSIONS
  PRP placed in the defects significantly enhanced bone healing in CSD in the calvaria of diabetic rats both qualitatively and quantitatively.
authors.affiliation: Division of Bucco-Maxillo-Facial Surgery and Traumatology, Department of Clinic and Surgery, Dental School, Federal University of Alfenas - UNIFAL, Alfenas, Brazil. michel_messora@terra.com.br
authors.forename: Ronaldo
authors.initials: R
authors.lastname: Mariano
authors.forename: Michel
authors.initials: M
authors.lastname: Messora
authors.forename: Adriana
authors.initials: A
authors.lastname: de Morais
authors.forename: Maria
authors.initials: M
authors.lastname: Nagata
authors.forename: Flávia
authors.initials: F
authors.lastname: Furlaneto
authors.forename: Cássia
authors.initials: C
authors.lastname: Avelino
authors.forename: Fernanda
authors.initials: F
authors.lastname: Paula
authors.forename: Sabrina
authors.initials: S
authors.lastname: Ferreira
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pinheiro
authors.forename: João Paulo
authors.initials: JP
authors.lastname: de Sene
mesh_terms: D000496:Alloxan; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000496:Alloxan