Muscarinic agonists acting through M2 acetylcholine receptors stimulate the migration of an NO-sensitive guanylyl cyclase to the plasma membrane of bovine tracheal smooth muscle.
Muscarinic agonists acting on bovine tracheal smooth muscle (BTSM) induce two separate cGMP signals, one at 20 sec associated with NO-sensitive-soluble-guanylyl-cyclase (NO-sGC) and another at 60 sec, linked to natriuretic-peptide-GC. The 20-sec-cGMP novel cascade starts with mAChRs, via unknown components, activates an NO-sGC. To unravel this cascade, in crude membranes isolated from intact BTSM strips exposed to muscarinic agonists, we detected GC activities increments at 20 sec and 60 sec. The 20-sec-GC is a NO-sensitive-GC, identified as alpha(1)beta(1)-heterodimer. In reconstitution experiments with purified plasma membranes and cytosol, muscarinic agonists induced an NO-sGC migration in a dose-dependent manner, being inhibited by muscarinic antagonists displaying an M(2)AChR profile and blocked by PTX, suggesting the involvement of G(o)/G(i) proteins. The NO-sGC related to migration was isolated and identified as an alpha(1)beta(1)-heterodimer. This work shows that muscarinic agonists in BTSM induce a massive and selective alpha(1)beta(1)-NO-sGC migration from cytoplasm to plasma membranes being responsible for the 20-sec-cGMP signal.
Uray
Fabiola Pláceres
FP
Sección de Biomembranas, Instituto de Medicina Experimental, Cátedra de Patología General y Fisiopatología, Escuela Luís Razetti, Facultad de Medicina. Universidad Central de Venezuela, Caracas, Venezuela.
de Alfonzo
Ramona González
RG
de Becemberg
Itala Lippo
IL
Alfonzo
Marcelo J
MJ
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology*; D000242:Cyclic AMP / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D015151:Immunoblotting; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology; D009569:Nitric Oxide / Q000378:metabolism*; D037342:Pertussis Toxin / Q000494:pharmacology; D043585:Receptor, Muscarinic M2 / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D000071756:Soluble Guanylyl Cyclase; D013347:Subcellular Fractions; D014132:Trachea / Q000187:drug effects* / Q000201:enzymology
D018721:Muscarinic Agonists; D043585:Receptor, Muscarinic M2; D018160:Receptors, Cytoplasmic and Nuclear; D043562:Receptors, G-Protein-Coupled; D009569:Nitric Oxide; D000242:Cyclic AMP; D037342:Pertussis Toxin; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase