Development and characterization of candidate rotavirus vaccine strains derived from children with diarrhoea in Vietnam.
In Vietnam, rotavirus infection accounts for more than one-half of all hospitalizations for diarrhoea among children less than 5 years of age. While new vaccines to prevent rotavirus diarrhoea have been developed and introduced into some countries by multinational manufacturers, the ability for developing countries such as Vietnam to introduce several new and important vaccines into the routine infant immunization schedule may be challenging. In order to be partially self-sufficient in vaccine production, Vietnam has pursued the development of several rotavirus strains as candidate vaccines using isolates obtained from Vietnamese children with diarrhoea. This paper describes the origin, isolation and characterization of 3 human rotavirus strains being considered for further vaccine development in Vietnam. The goal is to prepare a monovalent G1P [8] rotavirus vaccine using one of these strains obtained in Vietnam and naturally attenuated by multiple passages in cell culture. While this is an ambitious project that will require several years' work, we are using the lessons learned to improve the overall quality of vaccine production including the use of Vero cell techniques for the manufacture of other vaccines in Vietnam.
Luan
Le T
le T
Center for Research and Production of Vaccines and Biologicals (POLYVAC), 135 Loduc Street, Hai Ba Trung, Hanoi, Viet Nam. luanpolyvac@gmail.com
Trang
Nguyen V
NV
Phuong
Nguyen M
NM
Nguyen
Huong T
HT
Ngo
Huong T
HT
Nguyen
Huong T M
HT
Tran
Hanh B
HB
Dang
Ha N
HN
Dang
Anh D
AD
Gentsch
Jon R
JR
Wang
Yuhuan
Y
Esona
Mathew D
MD
Glass
Roger I
RI
Steele
A Duncan
AD
Kilgore
Paul E
PE
Nguyen
Man V
MV
Jiang
Baoming
B
Nguyen
Hien D
HD
D003967:Diarrhea / Q000517:prevention & control / Q000821:virology; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D010802:Phylogeny; D011786:Quality Control; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000276:immunology*; D017423:Sequence Analysis, RNA; D014744:Vietnam; D014776:Virus Cultivation
D012367:RNA, Viral; D022243:Rotavirus Vaccines