Molecular analysis and prognostic impact of the novel apoptotic gene BCL2L12 in gastric cancer.
Stomach cancer comprises a malignancy with feeble prognosis. In gastric carcinogenesis, molecular alterations in the apoptosis-related genes have been described. In this study, the expression of BCL2-like-12 (BCL2L12) gene, discovered and cloned by members of our group, was investigated in a statistically significant sample size of cancerous and non-cancerous stomach tissues and gastric cancer cells with quantitative real-time PCR methodology. BCL2L12 transcript was indicated in cancer gastric tissues to range from 29 to 53200 mRNA copies BCL2L12/10(6) mRNA copies GAPDH. Significant associations of BCL2L12 with gastric tumors of the early stages (I/II) (p=0.044) and of intestinal histotype (p=0.034) was substantiated. Both univariate and multivariate analyses disclosed, respectively, BCL2L12 relationship with disease-free (p=0.006 and p=0.025) and overall patients' survival (p=0.007 and p=0.022). Our results open new horizons for the possible application of BCL2L12 as a novel prognostic indicator of gastric cancer.
Florou
Dimitra
D
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, 15701 Athens, Greece.
Papadopoulos
Iordanis N
IN
Scorilas
Andreas
A
D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality* / Q000473:pathology
C430100:BCL2L12 protein, human; D014408:Biomarkers, Tumor; D009124:Muscle Proteins; D019253:Proto-Oncogene Proteins c-bcl-2