title: Enzyme-mediated protein refolding.
abstract: We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Okada
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Maruyama
authors.forename: Konomi
authors.initials: K
authors.lastname: Motomura
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kuroki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Maenaka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Sakono
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism; D008722:Methods; D017510:Protein Folding; D020673:Protein Renaturation; D012996:Solutions; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D012996:Solutions; D014508:Urea; D014510:Urease