title: Early expression of nucleolar SURF-6 protein in mouse spleen lymphocytes activated for proliferation in vitro.
abstract: Using specific antibodies we studied the content of nucleolar SURF-6 protein, which participates in rRNA processing, in mouser spleen lymphocytes activated for proliferation with concanavalin A and compared it with the content of nucleolar nucleophosmin/B23 protein and DNA replication factor PCNA, well-known markers of proliferating cells. Using immunocytochemistry and immunoblotting methods we demonstrate that the concentration of all these proteins increases simultaneously with increasing the proportion of proliferating cells. Unlike nucleophosmin/B23, SURF-6 protein was not revealed in quiescent lymphocyte nucleoli, while the increase of its level in activated lymphocytes preceded elevation of PCNA level. These observations suggest that nucleolar protein SURF-6 can act as a marker of early T lymphocyte activation for proliferation and that it could participate in cell cycle regulation in mammals.
authors.affiliation: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. a_moraleva@mail.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Moraleva
authors.forename: M V
authors.initials: MV
authors.lastname: Malysheva
authors.forename: Ch
authors.initials: Ch
authors.lastname: Magoulas
authors.forename: M A
authors.initials: MA
authors.lastname: Polzikov
authors.forename: O V
authors.initials: OV
authors.lastname: Zatsepina
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015151:Immunoblotting; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013154:Spleen / Q000166:cytology*
chemical_list: D008934:Mitogens; C000715570:Npm1 protein, mouse; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C098938:Surf6 protein, mouse; D003208:Concanavalin A; D000090243:Nucleophosmin