title: Synthesis, characterization, interaction with DNA and cytotoxicity in vitro of the complexes [M(dmphen)(CO3)].H2O [M=Pt(II), Pd(II)].
abstract: The complexes [Pt(dmphen)CO3].H2O (1), [Pd(dmphen)CO3].H2O (2) (dmphen is 2,9-dimethyl-1,10-phenanthroline) have been synthesized and characterized. The binding of the complexes with FS-DNA was investigated by UV spectrum and fluorescence spectrum, showing that the complexes have the ability of interaction with DNA of intercalative mode. The intrinsic binding constant K of the complexes with FS-DNA is 1.8 x 10(5) M(-1) (1) and 1.6 x 10(4) M(-1) (2), respectively. Gel electrophoresis assay demonstrated the ability of the complexes to cleave the pBR 322 plasmid DNA. Evaluation of cytotoxic activity of the complexes against four different cancer cell lines proved that the complexes exhibited cytotoxic specificity and significant cancer cell inhibitory rate.
authors.affiliation: Laboratory of Coordination Chemistry, Shenyang Institute of Chemical Technology, Shenyang 110142, China. ejgao@yahoo.com.cn
authors.forename: En-jun
authors.initials: EJ
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Ming-chang
authors.initials: MC
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Wan-zhong
authors.initials: WZ
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010165:Palladium / Q000737:chemistry*; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry
chemical_list: D009944:Organoplatinum Compounds; D014867:Water; D010165:Palladium; D004247:DNA