Inhibition of pro-inflammatory secreted phospholipase A2 by extracts from Cynara cardunculus L.
The aim of the present work was to evaluate the anti-inflammatory properties of Cynara cardunculus L. (Asteraceae) during its growth using various solvents such as n-hexane, dichloromethane, acetone, and methanol for air-dried leaves and stems. The anti-inflammatory activities of crude extracts were evaluated by measuring the inhibition potency of mammalian non-pancreatic phospholipases A2 (hG-IIA). The methanol and acetone extracts of leaves harvested in February exhibit potent inhibition of hG-IIA (IC(50) = 50 and 70 microg/ml, respectively). However, the acetone extract of stems harvested in December inhibits the hG-IIA with a lower IC(50) around 130 microg/ml. Fractionation on silica gel and hydrophobic gel of the methanol extract of leaves harvested in February increases the inhibitory effect, and the IC(50) reached 10 microg/ml.
Kammoun
Maher
M
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de soukra, BP1173-3038 Sfax, Tunisia.
Koubaa
Imed
I
Ben Ali
Yassine
Y
Jarraya
Raoudha
R
Gargouri
Youssef
Y
Damak
Mohamed
M
Bezzine
Sofiane
S
D000096:Acetone / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D031942:Cynara / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D054497:Phospholipases A2, Secretory / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D006586:Hexanes; D010936:Plant Extracts; D000096:Acetone; D054497:Phospholipases A2, Secretory; D000432:Methanol