[Isolation and identification of four DBP-degrading strains and molecular cloning of the degradation genes].
Four di-butyl-phthalate(DBP)-degrading bacterial strains, JDC-1, JDC-8, JDC-9 and JDC-12, were isolated from soil. The strains were gram positive. The 16S rRNA sequence analysis revealed that the four strains had similarities of 99% with Arthrobacter sp.. According to the morphologic, physiobiochemical characteristics and the analysis of their 16S rRNA, all the four strains were identified as Arthrobacter sp.. A 900 bp DNA fragment was obtained from the four strains by PCR amplified and clone. When compared with the large subunit of phthalate dioxygenase gene (phtA) of Arthrobacter keyseri, more than 96% similarities were evident in the nucleotide sequences. The optimal growth conditions and degradation rates of DBP were tested and the result indicated that the optimal growth conditions of the four bacteria strains were pH 7.0-8.5 and 30-35 degrees C. All the four bacteria strains performed efficiently for DBP degrading capabilities under optimal conditions. The most efficient strain JDC-1 degraded 500 mg/L DBP completely within 28 h whereas the least efficient strain JDC-8 degraded 500 mg/L DBP completely within 40 h. This study is helpful to the investigation of DBP-degrading mechanisms and the development of microbial resources.
Wu
Xue-Ling
XL
School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China. xueling0714@yahoo.com.cn
Jin
De-Cai
DC
Chao
Wei-Liang
WL
Liang
Ren-Xing
RX
Li
Qian
Q
Yang
Yu
Y
Qiu
Guan-Zhou
GZ
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D003993:Dibutyl Phthalate / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D003993:Dibutyl Phthalate; D010105:Oxygenases; C044732:phthalate dioxygenase