[Abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide].
OBJECTIVE
To investigate abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide.


METHODS
Sixty-nine workers with abnormal liver function in a synthetic leather factory were recruited as case. One hundred and twenty five control subjects with similar work tasks were selected from the same factory. Genotypes for GSTT1 and GSTM1 were determined by multiplex PCR, and for CYP2E1 PstI by PCR-RFLP assay.


RESULTS
The frequency of positive GSTM1 was 59.42% in cases and 38.40% in control, with an odds ratio (OR) of 2.34,95% CI: 1.29-4.29 (P=0.005). For GSTT1 and CYP2E1 PstI, the frequencies of genotypes showed no significant difference between case and control.


CONCLUSIONS
GSTM1 positive genotype may be genetic risk factors for development of abnormal liver function in workers exposed to N, N-dimethylformamide.
Xu
Cheng-Min
CM
Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Qian
Ya-Ling
YL
Zhu
Li-Jin
LJ
Xian
Ju-Xiang
JX
Chai
Jian-Rong
JR
Ruan
Zheng
Z
Zhang
Xing
X
D000328:Adult; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D004126:Dimethylformamide / Q000009:adverse effects*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects; D011110:Polymorphism, Genetic
D019392:Cytochrome P-450 CYP2E1; D004126:Dimethylformamide; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1