title: Growth and stratification of epithelial cells in minimal culture conditions.
abstract: Biological risk management is required in modern tissue engineering. Particular attention should be paid to the culture medium and the scaffold used. In this perspective, it is important to define minimal culture conditions which allow proper growth and differentiation of epithelial cells in vitro. We propose a simple experimental system which permits the generation of three-dimensional epidermal constructs using a collagen layer as a scaffold mimicking the entire dermal tissue and without the need of any feeder layer. Although showing significant differences compared to natural epidermis, these epidermal constructs appear useful to study keratinocyte differentiation and epidermis histogenesis.
authors.affiliation: Department of Experimental Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.forename: Andrea
authors.initials: A
authors.lastname: Casasco
authors.forename: Marco
authors.initials: M
authors.lastname: Casasco
authors.forename: Alberto
authors.initials: A
authors.lastname: Calligaro
authors.forename: Antonia Icaro
authors.initials: AI
authors.lastname: Cornaglia
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D023822:Tissue Engineering