title: Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis: proteomic analysis.
abstract: To detect serum biomarkers associated with disease activity in relapsing-remitting multiple sclerosis (MS). We studied serum low-molecular peptide profiling of MS patients and normal controls comprehensively by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Serum level of 1741 Da peptide was increased at the time of clinical relapse in patients than in normal controls and returned toward normal during remission. Tandem mass spectrometry analysis revealed that the peptide was a fragment of complement C4 (NGFKSHALQLNNRQI). This fragment peptide could be a possible marker of disease activity. It may reflect complement activation in the pathogenesis of MS.
authors.affiliation: Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. ssawai@yj8.so-net.ne.jp
authors.forename: Setsu
authors.initials: S
authors.lastname: Sawai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Umemura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Sei
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kodera
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kuwabara
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D003167:Complement Activation / Q000276:immunology; D003181:Complement C4 / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000276:immunology*; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D003181:Complement C4; D010446:Peptide Fragments