title: [siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis].
abstract: OBJECTIVE
  To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation.
  
  
  METHODS
  Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later.
  
  
  RESULTS
  In the oocytes without any siRNA injection, oocyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In oocytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01).
  
  
  CONCLUSIONS
  siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.
authors.affiliation: Center of Reproductive Medicine, Guanddong Academy of Medical Sciences (Guangdong Provincial People's Hospital), Guangzhou 510080, China. renlizhangm@yahoo.com.cn
authors.forename: Ren-Li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Jin-Xiu
authors.initials: JX
authors.lastname: Meng
authors.forename: An-Ming
authors.initials: AM
authors.lastname: Wen
authors.forename: Yue-Shen
authors.initials: YS
authors.lastname: Huang
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Liu
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Deng
authors.forename: Xi-Gu
authors.initials: XG
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D020552:Comet Assay; D005260:Female; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics* / Q000502:physiology; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D013108:Sphingomyelin Phosphodiesterase