Comparison of the immunological responses and efficacy of gamma-irradiated V3526 vaccine formulations against subcutaneous and aerosol challenge with Venezuelan equine encephalitis virus subtype IAB.
We recently developed a gamma-irradiation method to inactivate V3526, a live-attenuated Venezuelan equine encephalitis virus (VEEV) vaccine candidate. Dosage and schedule studies were conducted to evaluate the immunogenicity and efficacy of gamma-irradiated V3526 (gV3526). Subcutaneous (SC) and low dosage intramuscular (IM) administration of gV3526 were highly effective in protecting mice against a SC challenge with VEEV IA/B Trinidad Donkey strain, but not against an equivalent aerosol challenge. More robust immune responses and increased protective efficacy were noted when the IM dosage of gV3526 was increased. IM administration of gV3526 formulated with either CpG or CpG plus Alhydrogel further augmented the immune response in mice and resulted in 100% protection against aerosol challenge.
Martin
Shannon S
SS
DynPort Vaccine Company LLC (DVC), A CSC Company, Frederick, MD 21702, USA. smartin40@csc.com
Bakken
Russell R
RR
Lind
Cathleen M
CM
Garcia
Patricia
P
Jenkins
Erin
E
Glass
Pamela J
PJ
Parker
Michael D
MD
Hart
Mary Kate
MK
Fine
Donald L
DL
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004306:Dose-Response Relationship, Immunologic; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology* / Q000528:radiation effects; D004685:Encephalomyelitis, Venezuelan Equine / Q000276:immunology* / Q000517:prevention & control*; D005720:Gamma Rays; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D016019:Survival Analysis; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D038301:Virus Inactivation
D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D000536:Aluminum Hydroxide