Quantitative permeability imaging of plant tissues.
A method for mapping tissue permeability based on time-dependent diffusion measurements is presented. A pulsed field gradient sequence to measure the diffusion encoding time dependence of the diffusion coefficients based on the detection of stimulated spin echoes to enable long diffusion times is combined with a turbo spin echo sequence for fast NMR imaging (MRI). A fitting function is suggested to describe the time dependence of the apparent diffusion constant in porous (bio-)materials, even if the time range of the apparent diffusion coefficient is limited due to relaxation of the magnetization. The method is demonstrated by characterizing anisotropic cell dimensions and permeability on a subpixel level of different tissues of a carrot (Daucus carota) taproot in the radial and axial directions.
Sibgatullin
Timur A
TA
Laboratory of Biophysics and Wageningen NMR Centre, Wageningen University, 6703 HA Wageningen, The Netherlands.
Vergeldt
Frank J
FJ
Gerkema
Edo
E
Van As
Henk
H
D048429:Cell Size; D018552:Daucus carota / Q000166:cytology* / Q000378:metabolism*; D004058:Diffusion; D008279:Magnetic Resonance Imaging; D010539:Permeability; D013997:Time Factors