title: Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease.
abstract: In 2007, the islet community was notified that the collagenase product most commonly used for human islet isolations contained bovine neural tissue contaminants. To minimize this potential hazard, we adapted our human islet processing procedure to use a GMP-manufactured, bovine neural tissue-free collagenase blend. Here, we describe the factors that we consider most important for achieving reproducible and clinically useable islet isolations using this product.
authors.affiliation: Department of Internal Medicine, Diabetes Center, University of California-San Francisco, San Francisco, CA, USA.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Szot
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lee
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Tavakol
authors.forename: Jiena
authors.initials: J
authors.lastname: Lang
authors.forename: Florinna
authors.initials: F
authors.lastname: Dekovic
authors.forename: Robert K
authors.initials: RK
authors.lastname: Kerlan
authors.forename: Peter G
authors.initials: PG
authors.lastname: Stock
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Posselt
mesh_terms: D000328:Adult; D000818:Animals; D002417:Cattle; D017364:Collagenases / Q000592:standards*; D006801:Humans; D007515:Islets of Langerhans / Q000033:anatomy & histology; D016381:Islets of Langerhans Transplantation; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000592:standards*; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards; D009927:Tissue and Organ Procurement
chemical_list: D010447:Peptide Hydrolases; D017364:Collagenases