title: A comprehensive proteome map of bovine cerebrospinal fluid.
abstract: Cerebrospinal fluid (CSF) is considered as the most promising body fluid target for the discovery of biomarkers for early diagnosis of neurodegenerative diseases such as Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. For the recognition of disease-associated changes in bovine CSF protein patterns, a detailed knowledge of this proteome is a prerequisite. The absence of a high-resolution CSF proteome map prompted us to determine all bovine CSF protein spots that can be visualised on 2-D protein gels. Using state-of-the-art 2-DE technology for proteome mapping of bovine ante mortem CSF combined with sensitive fluorescent protein staining and MALDI-TOF/TOF MS for protein identification, a highly detailed 2-DE map of the bovine CSF proteome was established. Besides the proteins mapped by earlier studies, this map contains 66 different proteins, including 58 which were not annotated in bovine 2-DE CSF maps before.
authors.affiliation: Institute for Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Südufer 10, Greifswald-Insel Riems, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Brenn
authors.forename: Axel
authors.initials: A
authors.lastname: Karger
authors.forename: Martin
authors.initials: M
authors.lastname: Skiba
authors.forename: Ute
authors.initials: U
authors.lastname: Ziegler
authors.forename: Martin H
authors.initials: MH
authors.lastname: Groschup
mesh_terms: D000818:Animals; D002417:Cattle; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D020543:Proteome; D040901:Proteomics
chemical_list: D020543:Proteome