title: [Identification of metastasis-related microRNAs of hepatocellular carcinoma in hepatocellular carcinoma cell lines by quantitative real time PCR].
abstract: OBJECTIVE
  To identify the metastasis-related miRNAs in hepatocellular carcinoma (HCC) cell lines.
  
  
  METHODS
  A qRT-PCR method was established and optimized.
  
  
  RESULTS
  All candidate metastasis associated miRNAs except miR-124a were expressed in high metastasis cell line MHCC97H and low metastasis cell line MHCC97L, while some miRNAs were differentially expressed between liver cancer cell line (HepG2) and hepatic cell line (L02) (P less than 0.05), these miRNAs include: miR-148b (1.96+/-0.51 vs 3.76+/-0.28), miR-9 (-4.38+/-0.86 vs -1.10+/-0.53), miR-30c (8.41+/-0.40 vs 6.82+/-0.29), miR-338 (3.14+/-0.29 vs -2.36+/-0.32), miR-34a (0.71+/-0.40 vs -2.95+/-0.26), Let-7g (-4.07+/-0.55 vs -6.98+/-0.56). miR-148b expression was about 4 times higher than miR-148a [5.46 (IQR 4.25-6.67) vs 1.29 (IQR 0.94-1.64)] in all cell line tested (Z=-5.097, P=3x10(-7)).
  
  
  CONCLUSIONS
  This study may help to understand the biological significance of miRNAs in HCC metastasis.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hu-liang
authors.initials: HL
authors.lastname: Jia
authors.forename: Hai-jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Qiong-zhu
authors.initials: QZ
authors.lastname: Dong
authors.forename: Li-yun
authors.initials: LY
authors.lastname: Fu
authors.forename: Zhao-wei
authors.initials: ZW
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Ren
authors.forename: Qing-hai
authors.initials: QH
authors.lastname: Ye
authors.forename: Lun-xiu
authors.initials: LX
authors.lastname: Qin
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs