High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry.
A reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination in food of biogenic amines and their precursor amino acids after a precolumn derivatization with dansyl chloride. The chromatographic conditions, selected to be suitable for mass spectrometry detection, were optimized through experimental design and artificial neural networks. The HPLC-UV method was validated by comparing the separation results with those obtained through a HPLC method, working under the same chromatographic conditions but employing mass spectrometry detection. The HPLC-UV method was then applied to the analysis of different food samples, namely, cheese, clams, salami, and beer. For all of the matrices, recoveries (relative standard deviation always <5%) always >92% were obtained. The results are discussed as a function of the total biogenic amine content and of the concentration ratio between amines and precursor amino acids.
Mazzucco
Eleonora
E
DISAV Dipartimento di Scienze dell'Ambiente e della Vita, University of Piemonte Orientale Amedeo Avogadro, Viale Teresa Michel 11, 15121 Alessandria, Italy.
Gosetti
Fabio
F
Bobba
Marco
M
Marengo
Emilio
E
Robotti
Elisa
E
Gennaro
Maria Carla
MC
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001515:Beer / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis*; D049872:Bivalvia / Q000737:chemistry; D002611:Cheese / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D008461:Meat Products / Q000032:analysis; D012758:Shellfish / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000596:Amino Acids; D001679:Biogenic Amines