Temperature and photoperiod affect the endocrine disruption effects of ethinylestradiol, nonylphenol and their binary mixture in zebrafish (Danio rerio).
We found that temperature and photoperiod significantly influence the transcription of the estrogen-responsive genes, vitellogenin1 (Vtg1), vitellogenin2 (Vtg2), estrogen receptor-alpha (ER alpha) and estrogen receptor-beta (ER beta), after a 21-day exposure to environmentally relevant concentrations of 17 alpha-ethynylestradiol (EE2), nonylphenol (NP) and EE2 plus NP (EE2+NP). In general, gene transcription levels were higher as temperature and photoperiod length increased. The mRNA levels of Vtg1 in EE2 (10 ng/L) and EE2+NP (10 ng/L and 25 microg/L, respectively) groups were induced more than 10(4) times both in 21 degrees C-12L and 30 degrees C-14L groups, but only 369+/-23 and 178+/-59-fold induced in 12 degrees C-10L group compared to the control, respectively. Specifically, when exposed to a high concentration of NP (25 microg/L) for 21 days, the levels of all mRNAs examined were significantly increased (p<0.05) in the 21 degrees C-12L and the 30 degrees C-14L groups compared to the controls. However, no obvious induction in transcription was observed in the 12 degrees C-10L group. The results obtained in the present study clearly elucidate that temperature and photoperiod greatly influence the effect of EDCs, and thus suggest that to fully define the endocrine disruption effects seasonal and/or climate change effects must also be investigated.
Jin
Yuanxiang
Y
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Shu
Linjun
L
Sun
Liwei
L
Liu
Weiping
W
Fu
Zhengwei
Z
D000818:Animals; D004357:Drug Synergism; D052244:Endocrine Disruptors / Q000633:toxicity*; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D017440:Photoperiod; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors; D014819:Vitellogenins / Q000235:genetics; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics*
D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D010636:Phenols; D014819:Vitellogenins; D029961:Zebrafish Proteins; C486450:vtg1 protein, zebrafish; D004997:Ethinyl Estradiol; C025256:nonylphenol