Dammarane-type saponins from the flower buds of Panax ginseng and their effects on human leukemia cells.
Six dammarane-type saponins, including three new compounds, floralginsenosides Ta-Tc (1-3), and three known, floralginsenoside Td (4), ginsenoside F(1) (5), and ginsenoside F(5) (6), were isolated from the flower buds of Panax ginseng. Floralginsenoside Td (4) was first isolated from natural plant sources. Their structures were elucidated on the basis of extensive chemical and spectroscopic methods. Compounds 1, 5, and 6 showed cytotoxic activities towards the HL-60 human leukemia cell line with respective IC(50) values of 36.3, 23.2, and 62.4microM. In addition, after the HL-60 cells were treated with these compounds, several apoptosis events, including chromatin condensation and increase in the population of sub-G1 hypodiploid cells, were observed.
Nguyen
Huu Tung
HT
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Song
Gyu Yong
GY
Kim
Jeong-Ah
JA
Hyun
Jae-Hee
JH
Kang
Hee-Kyoung
HK
Kim
Young Ho
YH
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D035264:Flowers / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D014315:Triterpenes / Q000737:chemistry*; D000097669:Dammaranes
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes