title: Capillary chromatography based on the tube radial distribution of aqueous-organic mixture carrier solvents: effects of the inner-wall characteristics of the fused-silica tube on separation performance.
abstract: A capillary chromatography system was developed using an open capillary tube and a water-acetonitrile (hydrophilic)-ethyl acetate (hydrophobic) mixture carrier solution. In this study, we examined the effects of the inner-wall characteristics of a fused-silica capillary tube on the separation performance in this system. Untreated (silanol group-intact) and inactivated (silanol group-end-blocked) fused-silica capillary tubes were used. The mixture analyte of 1-naphthol and 2,6-naphthalenedisulfonic acid was injected into two types of capillary tubes. They were eluted from both tubes in this order with water-acetonitrile-ethyl acetate (volume ratio 2:7:4) mixture carrier solution, and eluted in reverse order with water-acetonitrile-ethyl acetate (volume ratio 15:3:2). The peak shapes observed here were analyzed while considering the inner-wall characteristics of the capillary tubes from the viewpoint of the tube radial distribution of the carrier solvents in the tubes.
authors.affiliation: Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Jinno
authors.forename: Ko
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi