Development of a radiolabeled beta-human chorionic gonadotropin.
beta-Human chorionic gonadotropin (beta-hCG) was successively labeled with [(67)Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM(-1), labeling efficiency 80 %). Preliminary in vivo studies (ID g(-1), %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported beta-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.
Jalilian
Amir R
AR
Nuclear Medicine Research Group Agricultural Medical and Industrial, Research School (AMIRS-NSTRI), Karaj, Iran. ajalilian@nrcam.org
Khoshdel
Mohammad Reza
MR
Garousi
Javad
J
Yousefnia
Hassan
H
Hosseini
Mohammad A
MA
Rajabifar
Saeed
S
Sardari
Daryoush
D
D000818:Animals; D001692:Biological Transport; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000737:chemistry* / Q000493:pharmacokinetics; D005708:Gallium; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D004369:Pentetic Acid; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals; D051381:Rats; D014018:Tissue Distribution
D018997:Chorionic Gonadotropin, beta Subunit, Human; D019275:Radiopharmaceuticals; C034163:gallium chloride; D004369:Pentetic Acid; D005708:Gallium