Development of a novel fluorometric assay for nitric oxide utilizing sesamol and its application to analysis of nitric oxide-releasing drugs.
Nitric oxide (NO) is related to various physiological effects as well as to numerous diseases caused by accentuation of NO production. Measurement of NO in cells and tissues is difficult as NO readily reacts with other molecules; furthermore, its half-life as a radical is fleeting. Currently, many NO pharmaceuticals are marketed as therapeutic agents for ischemic disease. Consequently, the identification of NO radicals and determination of generation rate from pharmaceuticals is very important when the effect of the medicinal supply is estimated. In this study, we developed a fluorometric assay for NO employing sesamol (3,4-methylenedioxyphenol) as a fluorometric substrate. Sesamol is converted to a fluorescent derivative (ex. 365 nm, em. 447 nm), which is dimmer in the presence of NO. The detection limit of NO with this method is 400 fmol; moreover, NO generated from drugs can be measured.
Abe
Seiji
S
School of Pharmacy, Showa University, Tokyo 142-8555, Japan.
Nakabayashi
Shigeo
S
Murayama
Jun-Ichiro
J
Sano
Yoshihiro
Y
Ohno
Ken-Ichi
K
Maeda
Masako
M
Arakawa
Hidetoshi
H
D000975:Antioxidants; D052117:Benzodioxoles; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D007511:Ischemia / Q000188:drug therapy; D009569:Nitric Oxide / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D010636:Phenols
D000975:Antioxidants; D052117:Benzodioxoles; D004364:Pharmaceutical Preparations; D010636:Phenols; D009569:Nitric Oxide; C025583:sesamol