title: In vivo monitor oxidative burst induced by Cd2+ stress for the oilseed rape (Brassica napus L.) based on electrochemical microbiosensor.
abstract: BACKGROUND
  Since the mechanism of Cd(2+) stress for plants is not clear, an in vivo method to monitor Cd(2+) stress for plants is necessary. However, oxidative burst (OB) is a signal messenger in the process of Cd(2+) stress for plants.
  
  
  OBJECTIVE
  To establish an electrochemical method with poly-o-phenylenediamine and Pt microparticle modified Pt electrode (POPD-Pt-MP-Pt) as a microbiosensor for the in vivo detection of oxidative burst induced by Cd(2+) stress in oilseed rape (Brassica napus L.).
  
  
  METHODS
  The optimal fabrication of POPD-Pt-MP-Pt biosensor was achieved. Electrochemical signal was collected by amperometry.
  
  
  RESULTS
  After oilseed rape was exposed to 84.9 mM CdCl(2) stress, three oxidative bursts were observed in oilseed rape by amperometry at 3.3 h, 8.4 h and 13.2 h, respectively. However, there was no obvious signal observed in the controlled assay.
  
  
  CONCLUSIONS
  This contribution presents the in vivo monitoring of the OB process induced by Cd(2+) stress in oilseed rape by POPD-Pt-MP-Pt microbiosensor in real-time. The novel electrochemical microbiosensor not only facilitates the real-time study in plant self-defence response to the adverse environment such as Cd(2+) stress, but also provides an effective tool for probing the self-defence mechanism in plants.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, People's Republic of China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D029688:Brassica napus / Q000187:drug effects* / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride / Q000633:toxicity; D002413:Cations, Divalent; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D010655:Phenylenediamines / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D016897:Respiratory Burst / Q000187:drug effects*; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D002413:Cations, Divalent; D010655:Phenylenediamines; C530487:poly(o-phenylenediamine); D002104:Cadmium; D010984:Platinum; D019256:Cadmium Chloride