title: Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs.
abstract: Elastin-like polymers are a new type of protein-based polymers that display interesting properties in the biomaterial field. Bone morphogenetic proteins (BMPs) are cytokines with a strong ability to promote new bone formation. In this work, we explored the use of elastin-like nanoparticles (average size 237.5+/-3.0 nm), created by thermoresponsive self-assembly, for the combined release of bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-14 (BMP-14). These BMPs could be encapsulated at high efficiency into the elastin-like particles and delivered in a sustained way for 14 days. The activity of the growth factors was retained, as shown by the induction of ALP activity and osteogenic mineralization in C2C12 cells. Increased bioactivity was observed with a combined release of BMP-2 and BMP-14. This approach shows a significant potential for future tissue engineering applications in bone.
authors.affiliation: 3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal. paulo.bessa@dep.uminho.pt
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Bessa
authors.forename: Raul
authors.initials: R
authors.lastname: Machado
authors.forename: Sylvia
authors.initials: S
authors.lastname: Nürnberger
authors.forename: Daniela
authors.initials: D
authors.lastname: Dopler
authors.forename: Asmita
authors.initials: A
authors.lastname: Banerjee
authors.forename: António M
authors.initials: AM
authors.lastname: Cunha
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Rodríguez-Cabello
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Martijn
authors.initials: M
authors.lastname: van Griensven
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Margarida
authors.initials: M
authors.lastname: Casal
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D016466:CHO Cells; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D004549:Elastin / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D005612:Freeze Drying; D055428:Growth Differentiation Factor 5 / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010316:Particle Size; D011994:Recombinant Proteins; D012995:Solubility; D013696:Temperature
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004337:Drug Carriers; C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D009842:Oligopeptides; D011994:Recombinant Proteins; D004549:Elastin; D000469:Alkaline Phosphatase