Detection of protein binding using activator generated by electron transfer for atom transfer radical polymerization.
A purge-free controlled living polymerization method, activator generated by electron transfer for atom transfer radical polymerization (AGET ATRP), is used to amplify the occurrence of protein binding events. Detection of ovalbumin is demonstrated where binding of femtomole protein is differentiable from the background using ellipsometry. Moreover, binding of subpicomole protein leads to visually distinguishable spots on the sensor surface within 15 min, which signifies its potential in future development of point-of-need devices.
Qian
Hong
H
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
He
Lin
L
D000818:Animals; D002417:Cattle; D004579:Electron Transport; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding
D055767:Immobilized Proteins; D011108:Polymers