Expression of Hsp72 protein in chronic kidney disease patients.
OBJECTIVE
Expression of heat shock protein (Hsp) 72 is one of the major mechanisms acting against cellular injury. It displays a plethora of functions, including a considerable impact on inflammation. The aim of this study was to investigate Hsp72 expression in blood monocytes of patients with chronic kidney disease (CKD).


METHODS
Hsp72 protein level was assessed by flow cytometry in blood monocytes of predialysis, haemodialysis (HD) and continuous peritoneal dialysis patients, and controls. It was followed by evaluation of Hsp72 gene expression in the same cohorts by reverse transcription-polymerase chain reaction.


RESULTS
The level of Hsp72 protein was significantly lower in the predialysis (359+/-83 AU) and HD groups (293+/-62 AU) than in controls (405+/-51 AU) (p<0.01 and p<0.001, respectively). The amount of mRNA was significantly lower only in the HD group, compared with controls (0.39+/-0.10 vs 0.48+/-0.10, p<0.01). In the predialysis group, there were negative correlations between Hsp72 protein level and serum creatinine concentration, blood urea nitrogen and C-reactive protein.


CONCLUSIONS
This study demonstrates that uraemic toxicity decreases expression of Hsp72. Attenuation of Hsp 72 expression in uraemia, found in the present study, could contribute to the inflammatory state, a common complication in CKD patients.
Marzec
Lukasz
L
Departments of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.
Zdrojewski
Zbigniew
Z
Liberek
Tomasz
T
Bryl
Ewa
E
Chmielewski
Michal
M
Witkowski
Jacek M
JM
Rutkowski
Boleslaw
B
D000368:Aged; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000379:methods; D011379:Prognosis; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050884:HSP72 Heat-Shock Proteins; D012313:RNA