title: Towards an optical biopsy for the diagnosis of breast cancer in vivo by endogenous fluorescence spectroscopy.
abstract: The techniques of medical imaging allow the detection of suspect lesions in the breast, but they do not always evidence the malignant nature of these lesions. Breast biopsies and histological analyses are therefore implemented to establish a diagnosis. In order to reduce the number of these invasive procedures, a portable clinical system was designed based upon the excitation of Endogenous Fluorescence in vivo at 405 nm via a fiber-optics probe included in a disposable needle of small diameter (<1 mm). From the fluorescence signal, the authors are able to discriminate between diseased and healthy areas of human breast biopsies. Stronger fluorescence intensity and systematic spectral red shift of the tumor areas were observed. These results are confirmed by confocal microscopy. This new instrument is promising for the minimally invasive diagnosis of breast tumors in vivo with an appreciable limitation of patient trauma and of operational and financial cost.
authors: Centre de Photonique Biomédicale, Centre Laser de l'Université Paris-Sud 11, Faculté des Sciences d'Orsay, Orsay cedex, France.
authors: Lama
authors: L
authors: Alchab
authors: Guillaume
authors: G
authors: Dupuis
authors: Corinne
authors: C
authors: Balleyguier
authors: Marie-Christine
authors: MC
authors: Mathieu
authors: Marie-Pierre
authors: MP
authors: Fontaine-Aupart
authors: René
authors: R
authors: Farcy
mesh_terms: D001706:Biopsy; D001943:Breast Neoplasms / Q000175:diagnosis*; D004867:Equipment Design; D005260:Female; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D006801:Humans; D018613:Microscopy, Confocal; D055095:Optics and Photonics; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods