Oxidant stress due to non ionic low osmolar contrast medium in rat kidney.
OBJECTIVE
Contrast media may cause contrast-induced nephropathy (CIN) in risk group. This study was taken up to establish possible effects of non ionic low osmolar contrast medium administration on oxidant/antioxidant status and nitric oxide (NO) levels in rat kidney tissues.


METHODS
Fourteen female, 14 wk old Wistar-albino rats were divided into 2 groups of 7 rats each (control and contrast groups). Non ionic low osmolar contrast medium was administered iv to the animals in the contrast group. The day after, animals were sacrificed and malondialdehyde (MDA) and NO levels and activities of antioxidant [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] and oxidant [xanthine oxidase (XO)] enzymes were measured in kidney tissues. Serum creatinine levels were measured to evaluate kidney functions.


RESULTS
Contrast medium administration caused an increase in MDA levels and a decrease in NO levels in kidney tissues.


CONCLUSIONS
The results suggest that non ionic low osmolar contrast medium administration leads to accelerated oxidant reactions and decreased NO level in rat kidney tissues. Further studies need to be done to assess the role of these changes in CIN.
Devrim
Erdinç
E
Ankara University Faculty of Medicine, Biochemistry Department, Turkey. devrim@medicine.ankara.edu.tr
Cetin
Meltem
M
Namuslu
Mehmet
M
Ergüder
Imge B
IB
Cetin
Recep
R
Durak
Ilker
I
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003287:Contrast Media / Q000009:adverse effects / Q000494:pharmacology; D003404:Creatinine / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009994:Osmolar Concentration; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D003287:Contrast Media; D016877:Oxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase