Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening.
The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (/r/ in <0.07, 0.34>), (/r/ in <0.01, 0.49>), respectively, as detected by both methods.
Hégrová
Blanka
B
Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Farková
Marta
M
Macuchová
Simona
S
Havel
Josef
J
Preisler
Jan
J
D000935:Antifungal Agents / Q000032:analysis; D023181:Antimicrobial Cationic Peptides / Q000032:analysis; D001515:Beer / Q000032:analysis* / Q000382:microbiology*; D002352:Carrier Proteins / Q000032:analysis; D018153:Czech Republic; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins; D005658:Fungi / Q000472:pathogenicity; D006360:Heat-Shock Proteins / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry* / Q000382:microbiology*; D010940:Plant Proteins / Q000032:analysis; D014674:Plant Proteins, Dietary / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D006360:Heat-Shock Proteins; C422456:Ltp1 protein, barley; D010940:Plant Proteins; D014674:Plant Proteins, Dietary; C036092:hordothionin protein, Hordeum vulgare