title: [Promoter recognition and beta-galactosidase reporter gene expression in Rhodococcus].
abstract: The genus Rhodococcus is of considerable interest in recent years, stemming from their diverse applications in biodegradation, bioremediation, biotransformation and biosurfactant. Using Nocardia/Rhodococcus-Escherichia coli shuttle plasmid pNV18.1 as the backbone vector, we tested the driven efficiency of promoters Ptac and PlacZ of E. coli and Pami-1/Pami-2 of R. ruber in host R. rhodochrous ATCC 33278 by overexpression of nitrile hydratase. Results showed that the specific activity of nitrile hydratase per dry cell weight in engineered Rhodococcus strains driven by Ptac, Pami-1, Pami-2 and PlacZ was 7.5, 6.3, 5.3 and 1.8 times of that in the wild, respectively. It indicated that these promoters could be well recognized by RNA polymerase of Rhodococcus. We further expressed the beta-galactosidase reporter gene (lacZ) in R. ruber driven by promoter PlacZ. Results indicated that lacZ was an appropriate reporter gene for genetic or metabolic engineering research of Rhodococcus.
authors: Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors: Changchun
authors: C
authors: Liu
authors: Huimin
authors: H
authors: Yu
authors: M
authors: M
authors: Yuchao
authors: Wenyu
authors: W
authors: Pan
authors: Hui
authors: H
authors: Luo
authors: Zhongyao
authors: Z
authors: Shen
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D001616:beta-Galactosidase