Expression profile of Notch-1 in mechanically overloaded plantaris muscle of mice.
OBJECTIVE
We investigated the expression pattern of Notch-1 in normal and hypertrophied plantaris muscle of mice.


METHODS
We performed immunofluorescence of both Notch-1 and the Notch-1-linking molecules.


RESULTS
Immunofluorescence labeling revealed Notch-1 protein in Pax7-positive satellite cells during days 2-6. We observed clear co-localization between Notch-1 and myogenin (4.9+/-1.3%) in the hypertrophied muscle at 4days. Several mononuclei (possibly satellite cells) possessed both Notch-1 and Foxo1 in the plantaris muscle subjected to mechanical overloading (4.1+/-1.2%).


CONCLUSIONS
Notch-1 may play an important role in the maintenance of quiescent satellite cells.
Akiho
Mai
M
Research Center for Physical Fitness, Sports and Health, Toyohashi, University of Technology, 1-1 Hibarigaoka, Tenpaku-cho, Toyohashi, 441-8580, Japan.
Nakashima
Hiroyuki
H
Sakata
Munehiro
M
Yamasa
Yuka
Y
Yamaguchi
Akihiko
A
Sakuma
Kunihiro
K
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000032:analysis / Q000378:metabolism; D005786:Gene Expression Regulation; D006984:Hypertrophy / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology*; D018008:Myogenin / Q000032:analysis / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000032:analysis / Q000378:metabolism; D051881:Receptor, Notch1 / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology; D016474:Weight-Bearing
D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D018008:Myogenin; C497762:Notch1 protein, mouse; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; D051881:Receptor, Notch1