title: Oxidative macromolecular damage in age-related macular degeneration.
abstract: OBJECTIVE
  To evaluate oxidative damage to the macromolecules, including protein, lipid, and DNA, in association with total oxidation status (TOS), total antioxidant capacity (TAC) in patients with exudative age-related macular degeneration (AMD).
  
  
  METHODS
  Serum levels of malondialdehyde (MDA) as an index of lipid peroxidation, protein carbonyl (PC) as a marker of protein oxidation, 8-hydroxy-29-deoxyguanosine (8-OHdG) as an indicator of oxidative DNA damage along with TOS, TAC were measured by specific methods in 47 patients with exudative AMD and 25 age- and sex-matched healthy subjects.
  
  
  RESULTS
  Significantly higher MDA, PC, 8-OHdG, TOS, and lower TAC levels were detected in the serum of patients with exudative AMD compared with their controls (p < 0.001).
  
  
  CONCLUSIONS
  The results indicate that an imbalance between TOS and TAC leads to not only increased lipid damage, but also protein and DNA damage. These first reported results suggest that protein and DNA damage might also play a role in the pathogenesis of AMD.
authors: Fatih University, Medical School, Department of Ophthalmology, Ankara, Turkey.
authors: Yüksel
authors: Y
authors: Totan
authors: Ramazan
authors: R
authors: Yağci
authors: Yavuz
authors: Y
authors: Bardak
authors: Hüseyin
authors: H
authors: Ozyurt
authors: Fadime
authors: F
authors: Kendir
authors: Gülsen
authors: G
authors: Yilmaz
authors: Semsettin
authors: S
authors: Sahin
authors: Ufuk
authors: U
authors: Sahin Tiğ
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D046911:Macromolecular Substances / Q000097:blood*; D008268:Macular Degeneration / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation
chemical_list: D000975:Antioxidants; D046911:Macromolecular Substances; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine