Conjugative transposition of Tn916 and detection of Tn916-Like transposon in Erysipelothrix rhusiopathiae.
In order to investigate the origin of tetracycline resistance in Erysipelothrix rhusiopathiae, conjugative transpositions of Tn916 were tested. The frequency of transfer between strains of E. rhusiopathiae was about 10-fold higher than that between Enterococcus faecalis and E. rhusiopathiae. In addition, detection of a Tn916-like transposon was performed by PCR assay and DNA sequencing in E. rhusiopathiae field isolates. Of 49 tetracycline-resistant isolates, 38 (77.6 %) carried a Tn916-like transposon, while 11 (22.4 %) carried tet(M) only. These results suggested that Tn916-like transposon may be widely present in the E. rhsuiopathiae field isolates resistant to tetracycline.
OZAWA
Manao
M
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo, Japan. ozawa@nval.go.jp
YAMAMOTO
Kinya
K
KOJIMA
Akemi
A
TAKAGI
Masami
M
TAKAHASHI
Toshio
T
D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004888:Erysipelothrix / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013752:Tetracycline / Q000494:pharmacology
D004251:DNA Transposable Elements; D013752:Tetracycline