[Mechanisms of nongenomic effect of 17beta-estradiol on human spermatozoa].
OBJECTIVE
To study the mechanisms of nongenomic effect of 17beta-estradiol on human spermatozoa.


METHODS
The intracellular calcium ([Ca2+]i) in the spermatozoa was measured by flow cytometry after the spermatozoa was treated with the inhibitors of trans-membrane signaling transduction pathways and impermeable 17beta-estradiol (E2-BSA). Western blot was used to detect the activation of the signal proteins after the spermatozoa was treated with 1 x 10(-6) mol/L E2-BSA and tamoxifen, an estrogen receptor inhibitor.


RESULTS
Adenylyl cyclase (AC) inhibitor SQ22536, phospholipase C (PLC) inhibitor U73122 and protein tyrosine kinase (TPK) inhibitor Genistein all deterred the increase of [Ca2+]i caused by E2-BSA. E2-BSA also increased the PLC protein and PKC protein significantly. Tamoxifen, an antagonist of estrogen receptor, did not inhibit the activation of PLC caused by E2-BSA.


CONCLUSIONS
The E2-BSA has an effect on human spermatozoa in a nongenomic pathway, possibly through the transmembrane signal transduction in relation to AC, PLC and TPK.
Gu
Xue-Bei
XB
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Zhang
Jin-Hu
JH
Yue
Li-Min
LM
Wang
Qiang
Q
Mao
Yong-Qiu
YQ
He
Ya-Ping
YP
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000328:Adult; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D004963:Estrenes / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011760:Pyrrolidinones / Q000494:pharmacology; D015398:Signal Transduction; D013094:Spermatozoa / Q000187:drug effects*; D013629:Tamoxifen / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
D000067956:Adenylyl Cyclase Inhibitors; D004963:Estrenes; D011760:Pyrrolidinones; D013629:Tamoxifen; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C017759:9-(tetrahydro-2-furyl)-adenine; D004958:Estradiol; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D000225:Adenine; D002118:Calcium