Floxin, a resource for genetically engineering mouse ESCs.
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
Singla
Veena
V
Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Hunkapiller
Julie
J
Santos
Nicole
N
Seol
Allen D
AD
Norman
Andrew R
AR
Wakenight
Paul
P
Skarnes
William C
WC
Reiter
Jeremy F
JF
D000483:Alleles; D000818:Animals; D001287:Attachment Sites, Microbiological / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D020224:Expressed Sequence Tags; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers; D005822:Genetic Vectors; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation
D005819:Genetic Markers; C045073:Cre recombinase; D019426:Integrases