title: DeNAno: Selectable deoxyribonucleic acid nanoparticle libraries.
abstract: DNA nanoparticles of approximately 250 nm were produced by rolling circle replication of circular oligonucleotide templates which results in highly condensed DNA particulates presenting concatemeric sequence repeats. Using templates containing randomized sequences, high diversity libraries of particles were produced. A biopanning method that iteratively screens for binding and uses PCR to recover selected particles was developed. The initial application of this technique was the selection of particles that bound to human dendritic cells (DCs). Following nine rounds of selection the population of particles was enriched for particles that bound DCs, and individual binding clones were isolated and confirmed by flow cytometry and microscopy. This process, which we have termed DeNAno, represents a novel library technology akin to aptamer and phage display, but unique in that the selected moiety is a multivalent nanoparticle whose activity is intrinsic to its sequence. Cell targeted DNA nanoparticles may have applications in cell imaging, cell sorting, and cancer therapy.
authors: Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
authors: Jason M
authors: JM
authors: Steiner
authors: Marta
authors: M
authors: Sartor
authors: Ana B
authors: AB
authors: Sanchez
authors: Davorka
authors: D
authors: Messmer
authors: Anna
authors: A
authors: Freed
authors: Sadik
authors: S
authors: Esener
authors: Bradley T
authors: BT
authors: Messmer
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002999:Clone Cells; D004247:DNA / Q000032:analysis*; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D053758:Nanoparticles / Q000032:analysis*
chemical_list: D004247:DNA