title: Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2).
abstract: Overexpression of antisense chromosomal cis-encoded noncoding RNAss (ncRNAs) in glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including several ncRNAs that are differentially expressed in bacterial hormone-defective mutants.
authors: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
authors: Davide
authors: D
authors: D'Alia
authors: Kay
authors: K
authors: Nieselt
authors: Stephan
authors: S
authors: Steigele
authors: Jonas
authors: J
authors: Müller
authors: Ilse
authors: I
authors: Verburg
authors: Eriko
authors: E
authors: Takano
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015153:Blotting, Western; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D016372:RNA, Antisense; D012329:RNA, Bacterial; D022661:RNA, Untranslated; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase