title: Influence of intra-articular neutrophils on the effects of photodynamic therapy for murine MRSA arthritis.
abstract: Although there have been some reports about the cytotoxic effects of photodynamic therapy (PDT) on multidrug-resistant bacteria, there have been few reports in which favorable results of PDT on a local infection site are described. This study aimed to verify the hypothesis that the low efficacy of PDT on a local infection site is due to the cytotoxic effect of PDT on leukocytes. PDT using Photofrin exerted significant cytotoxicity for cultured methicillin-resistant Staphylococcus aureus (MRSA). Nevertheless, this therapeutic modality was not effective for a murine MRSA arthritis model. Approximately 30% of intra-articular leukocytes, mainly neutrophils, died immediately after PDT, and a further decrease in the number of intra-articular leukocytes and atrophy of the synovial tissue were seen 24 h after PDT. Isolated peripheral neutrophils showed significant affinity for Photofrin and showed significant morphological damage, resulting in cell death, when they were subject to PDT using Photofrin. These results indicate that intra-articular neutrophils have an influence on the effects of PDT for MRSA arthritis.
authors: Department of Orthopaedic Surgery, National Defense Medical College, Namiki, Tokorozawa, Japan.
authors: Masamitsu
authors: M
authors: Tanaka
authors: Manabu
authors: M
authors: Kinoshita
authors: Yasuo
authors: Y
authors: Yoshihara
authors: Nariyoshi
authors: N
authors: Shinomiya
authors: Shuhji
authors: S
authors: Seki
authors: Koichi
authors: K
authors: Nemoto
authors: Yuji
authors: Y
authors: Morimoto
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use; D001168:Arthritis / Q000150:complications / Q000628:therapy*; D016923:Cell Death; D017323:Dihematoporphyrin Ether / Q000009:adverse effects / Q000627:therapeutic use; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000528:radiation effects; D051379:Mice; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D010778:Photochemotherapy / Q000009:adverse effects*; D013203:Staphylococcal Infections / Q000628:therapy
chemical_list: D000890:Anti-Infective Agents; D017323:Dihematoporphyrin Ether