title: Single-Channel Recording of TASK-3-like K Channel and Up-Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons.
abstract: Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K(+) channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K(+) channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K(+) channel was 33.0+/-0.1 pS at -60 mV, and TASK-3 activity fell by 65+/-5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K(+) channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K(+) channel under SCI-induced acidic condition.
authors: Department of Thoracic Surgery, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 660-751, Korea.
authors: Inseok
authors: I
authors: Jang
authors: Jun-Ho
authors: JH
authors: La
authors: Gyu-Tae
authors: GT
authors: Kim
authors: Jeong-Soon
authors: JS
authors: Lee
authors: Eun-Jin
authors: EJ
authors: Kim
authors: Eun-Shin
authors: ES
authors: Lee
authors: Su-Jeong
authors: SJ
authors: Kim
authors: Jeong-Min
authors: JM
authors: Seo
authors: Sang-Ho
authors: SH
authors: Ahn
authors: Jae-Yong
authors: JY
authors: Park
authors: Seong-Geun
authors: SG
authors: Hong
authors: Dawon
authors: D
authors: Kang
authors: Jaehee
authors: J
authors: Han