[Study on antiangiogenesis effect of Vaccaria segetalis].
OBJECTIVE
To study the inhibiting effect of Vaccaria segetalis extracts on neovascularization.


METHODS
The effect of Vaccaria segetalis extracts on the proliferation, migration in vitro and tube formation on Matrigel of endothelial cell (HMEC) in vivo were examined by MTT assay and Matrigel plug assay.


RESULTS
The proliferation and migration of HMEC were inhibited significantly by Vaccaria segetalis extracts in a dose-dependent manner (IC50 = 50 microg/mL). It also inhibited angiogenesis in Matrigel plug mouse model.


CONCLUSIONS
Vaccaria segetalis extracts can inhibit angiogenensis obviously, and it could be developed as an effective antiangiogenic drug.
Feng
Lei
L
Laboratory of Natural Medicine, Ministry of Education, Jiangnan University, Wuxi 214122, China. feng2000lei@yahoo.com.cn
Hua
Hui
H
Qiu
Li-Ying
LY
Zhang
Lian-Fen
LF
Jin
Jian
J
D020533:Angiogenesis Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D042783:Endothelial Cells / Q000187:drug effects*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007797:Laminin; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011509:Proteoglycans; D011897:Random Allocation; D031245:Vaccaria / Q000737:chemistry*
D020533:Angiogenesis Inhibitors; D004338:Drug Combinations; D007797:Laminin; D010936:Plant Extracts; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen