Trichophyton rubrum and Trichophyton interdigitale: genetic diversity among species and strains by random amplified polymorphic DNA method.
Onychomycosis is a common condition that represents up to 50% of all nail problems and 30% of all cases of dermatophytoses. Trichophyton rubrum and Trichophyton interdigitale are the most common agents involved in this condition. In cases of recurrent post-treatment onychomycosis, strain fingerprinting could reveal whether the original isolate is responsible, a new strain has been acquired or if multiple strains are involved. The aim of this study was to evaluate the efficacy of the RAPD method for species and strain differentiation of T. rubrum and T. interdigitale obtained from patients with subungeal distal-lateral onychomycosis. A set of 86 strains of onychomycosis causative dermatophytes were submitted to species differentiation and strain typing by RAPD method with two previously described primers. Both primers proved capable of strain differentiation when tested for each species. Nineteen molecular profiles were configured for T. rubrum isolates with primers 1 and 6. For T. mentagrophytes, ten molecular profiles were configured with primer 1 and twenty-one with primer 6. We found that T. interdigitale and T. rubrum species were grouped in different clusters when both primers were analyzed together. This study shows that these primers are valuable tools for strain differentiation with T. rubrum and T. intedigitale.
Santos
Daniel Assis
DA
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, PO Box 486, Belo Horizonte, Minas Gerais, Brazil. danielsantosassis@yahoo.com.br
Araújo
Roberta Amália Carvalho
RA
Hamdan
Júnia Soares
JS
Cisalpino
Patrícia Silva
PS
D016000:Cluster Analysis; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques; D014009:Onychomycosis / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D014249:Trichophyton / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D004271:DNA, Fungal