title: Comparison of gene expression profiles between human and mouse monocyte subsets.
abstract: Blood of both humans and mice contains 2 main monocyte subsets. Here, we investigated the extent of their similarity using a microarray approach. Approximately 270 genes in humans and 550 genes in mice were differentially expressed between subsets by 2-fold or more. More than 130 of these gene expression differences were conserved between mouse and human monocyte subsets. We confirmed numerous of these differences at the cell surface protein level. Despite overall conservation, some molecules were conversely expressed between the 2 species' subsets, including CD36, CD9, and TREM-1. Other differences included a prominent peroxisome proliferator-activated receptor gamma (PPARgamma) signature in mouse monocytes, which is absent in humans, and strikingly opposed patterns of receptors involved in uptake of apoptotic cells and other phagocytic cargo between human and mouse monocyte subsets. Thus, whereas human and mouse monocyte subsets are far more broadly conserved than currently recognized, important differences between the species deserve consideration when models of human disease are studied in mice.
authors: Department of Gene and Cell Medicine and Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA.
authors: Molly A
authors: MA
authors: Ingersoll
authors: Rainer
authors: R
authors: Spanbroek
authors: Claudio
authors: C
authors: Lottaz
authors: Emmanuel L
authors: EL
authors: Gautier
authors: Marion
authors: M
authors: Frankenberger
authors: Reinhard
authors: R
authors: Hoffmann
authors: Roland
authors: R
authors: Lang
authors: Muzlifah
authors: M
authors: Haniffa
authors: Matthew
authors: M
authors: Collin
authors: Frank
authors: F
authors: Tacke
authors: Andreas J R
authors: AJ
authors: Habenicht
authors: Loems
authors: L
authors: Ziegler-Heitbrock
authors: Gwendalyn J
authors: GJ
authors: Randolph
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis