Packed-bed reactor running on babassu oil and glycerol to produce monoglycerides by enzymatic route using immobilized Burkholderia cepacia lipase.
The aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. The reactor operated continuously for 22 days at 50 degrees C, and during the first 6 days, no significant decrease on the initial lipase activity was observed. Monoglycerides concentration was in the range from 25 to 33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivation profile described by Arrhenius model estimated values of 50 days and 1.37 x 10(-2) h(-1) the half-life and deactivation coefficient, respectively.
de Freitas
Larissa
L
Engineering School of Lorena, University of São Paulo, P.O. Box 116-12602-810, Lorena, São Paulo, Brazil.
dos Santos
Julio C
JC
Zanin
Gisella Maria
GM
de Castro
Heizir F
HF
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D016956:Burkholderia cepacia / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*
D004800:Enzymes, Immobilized; D050178:Monoglycerides; D010938:Plant Oils; D008049:Lipase; D005990:Glycerol