[Determination of adenosine in food by high performance anion exchange chromatography].
OBJECTIVE
To establish a high performance anion chromatography method for the determination of adenosine in food.


METHODS
Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0.25 mol/L NaOH as the mobile phase. A pulse ampere detector with Au as working electrode was used throughout the experiment.


RESULTS
A good linear result was produced for adenosine in the range of 1.00-20.00 microg/mL. The inner-day RSD of peak area and retention time for the standard adenosine solution were 4.97% and 0.47%, respectively. The intra-day RSD of peak area and retention time for the standard adenosine solution were 6.08% and 0.52%, respectively. The proposed method was applied to food and satisfactory results were obtained, with and average recoveries of 93.3%-106.7% and 8.86% of RSD.


CONCLUSIONS
The high performance anion exchange chromatography method is a simple, accurate, and reliable method for measuring adenosine in food, which can be completed within 10 minutes.
Zou
Xiao-Li
XL
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu, China.
Zeng
Hong-Yan
HY
Li
Yuan-Qian
YQ
Mei
Tang-Lin
TL
Jiang
Bo
B
D000241:Adenosine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*
D005503:Food Additives; D000241:Adenosine