Intraocular antibody synthesis against rubella virus and other microorganisms in Fuchs' heterochromic cyclitis.
BACKGROUND
Fuchs' heterochromic cyclitis (FHC) is a common intraocular disease of uncertain etiology that has recently been related to rubella virus (RV) infection.


METHODS
We investigated the synthesis of RV-specific IgG using aqueous humor and serum samples from 63 consecutive patients with FHC. In addition, intraocular immunoglobulin G production against herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV) and toxoplasma gondii was determined. In 20 patients, the detection of RV RNA was additionally performed by RT-PCR on the E1 gene. Forty-six patients with HSV- and VZV-associated uveitis, HLA B-27 positive anterior uveitis, and Posner-Schlossman syndrome served as controls.


RESULTS
Specific intraocular antibody synthesis against RV was confirmed in all 63 FHC patients, whereas none of the 46 controls was positive for RV IgG. Interestingly, in 11 patients with positive RV IgG synthesis, additional HSV (eight), VZV (one) and CMV (two) specific antibodies could be detected. Only twice was viral RNA detectable by PCR in a patient with FHC.


CONCLUSIONS
In this largest reported series of FHC patients, we detected a strong association between FHC and intraocular antibody synthesis against rubella virus. Furthermore, in 11 patients, it was possible to confirm an additional intraocular antibody synthesis, in particular HSV. PCR-positive results in the aqueous humor were exclusively obtained for RV. In contrast to other studies, the RV genome could only be identified in two patients (10%).
Ruokonen
Peter C
PC
Augenklinik Charité-Universitätsmedizin Berlin (CVK) Germany, Augustenburger Platz 1, 13353 Berlin, Germany. peter.ruokonen@charite.de
Metzner
Sylvia
S
Ucer
Aylin
A
Torun
Necip
N
Hofmann
Jörg
J
Pleyer
Uwe
U
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000913:Antibodies, Protozoan / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D001082:Aqueous Humor / Q000276:immunology / Q000821:virology; D002648:Child; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015828:Eye Infections, Viral / Q000276:immunology* / Q000821:virology; D005260:Female; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D015863:Iridocyclitis / Q000276:immunology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012409:Rubella / Q000276:immunology* / Q000821:virology; D012412:Rubella virus / Q000235:genetics / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics; D055815:Young Adult
D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus