Adaptive Treg generation by DCs and their functional analysis.
Naturally occurring thymus-derived CD4(+)CD25(+)Foxp3(+) regulatory T cells (nTregs) are critical regulators of immune tolerance. Foxp3(+) Tregs can also be induced from CD25(-) naïve CD4 T cells in vivo and ex vivo. This conversion process requires cytokines such as IL2 and TGFbeta as well as suboptimal TCR stimulation, thus is regulated by the co-stimulatory status of antigen-presenting cells (i.e., dendritic cells). Although mature dendritic cells (DCs) are potent initiators of adaptive immune response, immature steady-state DCs contribute to immune tolerance. In this chapter, we summarize methods that use ex vivo splenic DCs to induce the conversion of naïve CD4(+) T cells to adaptive Foxp3(+)CD4(+) regulatory T cells (aTreg) in the presence of TGFbeta.
Wang
Li
L
Dartmouth Medical School, Norris Cotton Cancer Center, Lebanon, NH, USA.
D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology*; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D015704:CD4 Antigens; D016827:CD8 Antigens; C431698:CD8alpha antigen; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D016212:Transforming Growth Factor beta