title: Purification of genomic DNA with minimal contamination of proteins.
abstract: The purification is based on a set of solutions and a simple centrifugation procedure. Protocols are designed for an easy extraction and purification of genomic DNA from a wide range of samples, including whole blood, buffy coat, bone marrow, body fluids, buccal cells, tissues, mouse tails, etc. RBCs are lysed by dilution into a hypotonic solution. Tissues are broken down and digested by proteinase K in the presence of an anion detergent to release genomic DNA. After precipitation of the detergent and proteins, unique beads that bind proteins, lipids, and RNAs are added to achieve the supreme purity. Genomic DNA is then separated by alcohol precipitation. A proprietary nucleic acid precipitation reagent is used to enhance DNA recovery from low concentration samples. No DNA-binding beads or columns are used in the method, eliminating the problem of low yield and the risk of shearing of genomic DNA. The purified samples are free of proteins, lipids, salts, and RNA contamination. Purified samples are also stable for storage and suitable for all downstream applications.
authors: EdgeBio, Gaithersburg, Maryland 20877, USA.
authors: Haroun R
authors: HR
authors: Hebron
authors: Yu
authors: Y
authors: Yang
authors: Jun
authors: J
authors: Hang
mesh_terms: D000838:Anions; D000925:Anticoagulants / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D016678:Genome; D006801:Humans; D008055:Lipids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012313:RNA / Q000737:chemistry
chemical_list: D000838:Anions; D000925:Anticoagulants; D003902:Detergents; D008055:Lipids; D011506:Proteins; D000431:Ethanol; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D019286:Endopeptidase K