title: Electro-optical imaging microscopy of dye-doped artificial lipidic membranes.
abstract: Artificial lipidic bilayers are widely used as a model for the lipid matrix in biological cell membranes. We use the Pockels electro-optical effect to investigate the properties of an artificial lipidic membrane doped with nonlinear molecules in the outer layer. We report here what is believed to be the first electro-optical Pockels signal and image from such a membrane. The electro-optical dephasing distribution within the membrane is imaged and the signal is shown to be linear as a function of the applied voltage. A theoretical analysis taking into account the statistical orientation distribution of the inserted dye molecules allows us to estimate the doped membrane nonlinearity. Ongoing extensions of this work to living cell membranes are discussed.
authors: Laboratoire de Photonique Quantique et Moléculaire, Centre National de la Recherche Scientifique, UMR8537, d'Alembert Institute, Ecole Normale Supérieure de Cachan, Cachan, France. bassamhajj85@hotmail.com
authors: Bassam
authors: B
authors: Hajj
authors: Sophie
authors: S
authors: De Reguardati
authors: Loïc
authors: L
authors: Hugonin
authors: Bruno
authors: B
authors: Le Pioufle
authors: Toshihisa
authors: T
authors: Osaki
authors: Hiroaki
authors: H
authors: Suzuki
authors: Shoji
authors: S
authors: Takeuchi
authors: Halina
authors: H
authors: Mojzisova
authors: Dominique
authors: D
authors: Chauvat
authors: Joseph
authors: J
authors: Zyss
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008564:Membrane Potentials; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques; D008853:Microscopy; D057054:Molecular Imaging; D055620:Optical Phenomena; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D011199:Potentiometry; D011726:Pyridinium Compounds / Q000378:metabolism
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D004396:Coloring Agents; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011726:Pyridinium Compounds