title: Serotonin transport and metabolism in the mammary gland modulates secretory activation and involution.
abstract: BACKGROUND
  Serotonin [5-hydroxytryptamine (5-HT)] is an important local regulator of lactation homeostasis; however, the roles for the serotonin reuptake transporter and monoamine oxidase have not been known.
  
  
  OBJECTIVE
  The aim of the study was to determine whether drugs that impact 5-HT affect human lactation physiology.
  
  
  METHODS
  We conducted laboratory studies of human and animal models and an observational study of the onset of copious milk secretion in postpartum women at a university medical center.
  
  
  METHODS
  We studied women expecting their first live-born infant; exclusion criteria were: referred to the medical center for another medical condition, known contraindication to breastfeed, and less than 19 yr of age and unable to obtain parental consent.
  
  
  METHODS
  The mothers were interviewed. The cell and animal studies consisted of a variety of biochemical, pharmacological, and genetic interventions.
  
  
  METHODS
  The human subjects outcome was prevalence of delayed onset of copious milk secretion. The cell and animal outcomes were physiological and morphological.
  
  
  RESULTS
  Inhibiting serotonin reuptake in mammary epithelial cells altered barrier function, and the effects were amplified by coadministering a monoamine oxidase inhibitor. Direct delivery of fluoxetine by slow-release pellets caused localized involution. TPH1 knockout mice displayed precocious secretory activation. Among a cohort of 431 women, those taking SSRI were more likely (P = 0.02) to experience delayed secretory activation.
  
  
  CONCLUSIONS
  Medications that perturb serotonin balance dysregulate lactation, and the effects are consistent with those predicted by the physiological effects of intramammary 5-HT bioactivity. Mothers taking serotonergic drugs may need additional support to achieve their breastfeeding goals.
authors: 231 Albert Sabin Way, Molecular and Cellular Physiology, Cincinnati, Ohio 45267-0576, USA.
authors: Aaron M
authors: AM
authors: Marshall
authors: Laurie A
authors: LA
authors: Nommsen-Rivers
authors: Laura L
authors: LL
authors: Hernandez
authors: Kathryn G
authors: KG
authors: Dewey
authors: Caroline J
authors: CJ
authors: Chantry
authors: Karen A
authors: KA
authors: Gregerson
authors: Nelson D
authors: ND
authors: Horseman
mesh_terms: D000818:Animals; D001692:Biological Transport; D001940:Breast / Q000378:metabolism*; D002478:Cells, Cultured; D017097:Electric Impedance; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D006801:Humans; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008995:Monoamine Oxidase / Q000502:physiology; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000032:analysis / Q000502:physiology; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D014365:Tryptophan Hydroxylase / Q000502:physiology
chemical_list: D005473:Fluoxetine; D008995:Monoamine Oxidase; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors; D014365:Tryptophan Hydroxylase; C487801:Tph1 protein, mouse