title: Serum biomarkers of renal cell carcinoma assessed using a protein profiling approach based on ClinProt technique.
abstract: OBJECTIVE
  To investigate the possibility of using the ClinProt technique to find serum cancer related diagnostic markers that are able to better discriminate healthy subjects from patients affected by renal cell carcinoma (ccRCC). Renal cell carcinoma is the most common malignancy of the kidney. Biomarkers for early detection, prognosis, follow-up, and differential diagnosis of ccRCC from benign renal lesions are needed in daily clinical practice when imaging is not helpful.
  
  
  METHODS
  Serum of 29 healthy subjects and 33 ccRCC patients was analyzed by the ClinProt/MALDI-ToF technique.
  
  
  RESULTS
  A cluster of 3 peptides (A = m/z 1083 +/- 8 Da, B = m/z 1445 +/- 8 Da and C = m/z 6879 +/- 8 Da) was able to discriminate patients from control subjects. Cross-validation analysis using the whole casistic showed 88% and 96% of sensitivity and specificity, respectively. Moreover, the cluster showed 100% sensitivity for the identification of patients at pT2 (n = 5) and pT3 (n = 8) and 85% for pT1 patients (n = 20). The intensity of peaks A and C continuously decreased from pT1 to pT3, whereas peak B increased in pT1 and pT2.
  
  
  CONCLUSIONS
  These results may be useful to set up new diagnostic or prognostic tools.
authors: Department of Experimental Medicine, University of Milano-Bicocca, Monza, Italy.
authors: Clizia
authors: C
authors: Chinello
authors: Erica
authors: E
authors: Gianazza
authors: Italo
authors: I
authors: Zoppis
authors: Veronica
authors: V
authors: Mainini
authors: Carmen
authors: C
authors: Galbusera
authors: Stefano
authors: S
authors: Picozzi
authors: Francesco
authors: F
authors: Rocco
authors: Giacomo
authors: G
authors: Galasso
authors: Silvano
authors: S
authors: Bosari
authors: Stefano
authors: S
authors: Ferrero
authors: Roberto
authors: R
authors: Perego
authors: Francesca
authors: F
authors: Raimondo
authors: Cristina
authors: C
authors: Bianchi
authors: Marina
authors: M
authors: Pitto
authors: Stefano
authors: S
authors: Signorini
authors: Paolo
authors: P
authors: Brambilla
authors: Paolo
authors: P
authors: Mocarelli
authors: Marzia
authors: M
authors: Galli Kienle
authors: Fulvio
authors: F
authors: Magni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor