Lippia origanoides chemotype differentiation based on essential oil GC-MS and principal component analysis.
Chromatographic (GC/flame ionization detection, GC/MS) and statistical analyses were applied to the study of essential oils and extracts obtained from flowers, leaves, and stems of Lippia origanoides plants, growing wild in different Colombian regions. Retention indices, mass spectra, and standard substances were used in the identification of 139 substances detected in these essential oils and extracts. Principal component analysis allowed L. origanoides classification into three chemotypes, characterized according to their essential oil major components. Alpha- and beta-phellandrenes, p-cymene, and limonene distinguished chemotype A; carvacrol and thymol were the distinctive major components of chemotypes B and C, respectively. Pinocembrin (5,7-dihydroxyflavanone) was found in L. origanoides chemotype A supercritical fluid (CO(2)) extract at a concentration of 0.83+/-0.03 mg/g of dry plant material, which makes this plant an interesting source of an important bioactive flavanone with diverse potential applications in cosmetic, food, and pharmaceutical products.
Stashenko
Elena E
EE
Chromatography Laboratory, Research Center of Excellence, CENIVAM, Industrial University of Santander, Bucaramanga, Colombia. elena@tucan.uis.edu.co
Martínez
Jairo R
JR
Ruíz
Carlos A
CA
Arias
Ginna
G
Durán
Camilo
C
Salgar
William
W
Cala
Mónica
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003105:Colombia; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D032411:Lippia / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D025341:Principal Component Analysis / Q000379:methods*
D009822:Oils, Volatile; D010938:Plant Oils