Parylene-A coated microplate for covalent immobilization of proteins and peptides.
Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.
Jeon
Byung-Jin
BJ
Department of Materials Science and Engineering, Yonsei University, 134 Shin-chon-dong, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea.
Kim
Moo-Hwan
MH
Pyun
Jae-Chul
JC
D001665:Binding Sites; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008904:Miniaturization; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D055620:Optical Phenomena; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D014992:Xylenes / Q000737:chemistry* / Q000378:metabolism
D009842:Oligopeptides; D011108:Polymers; D014992:Xylenes; C011055:parylene; D006735:Horseradish Peroxidase