title: Assembly, tuning and use of an apertureless near field infrared microscope for protein imaging.
abstract: This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented.
authors: Department of Chemistry, University of Toronto, Toronto, Canada.
authors: Melissa
authors: M
authors: Paulite
authors: Zahra
authors: Z
authors: Fakhraai
authors: Boris B
authors: BB
authors: Akhremitchev
authors: Kerstin
authors: K
authors: Mueller
authors: Gilbert C
authors: GC
authors: Walker
mesh_terms: D000682:Amyloid / Q000737:chemistry; D007259:Infrared Rays; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
chemical_list: D000682:Amyloid; D011506:Proteins