Detection of pandemic influenza A/H1N1/2009 virus by real-time reverse transcription polymerase chain reaction.
The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection of this new infectious agent. A new method was developed for detection of the pandemic influenza A/H1N1/2009 using real-time reverse transcription polymerase chain reaction (RT-PCR). Direct comparison with the specific primers and probes recommended by the World Health Organization (WHO) demonstrates that the new method has a much greater sensitivity, and may thus be employed as a rapid, alternative method in detecting of the pandemic influenza A/H1N1/2009 virus.
Wu
Weili
W
Beijing Institute of Genomics, Chinese Academy of Sciences, Airport Industry B6, Beijing 101300, China.
Kang
Xiaoping
X
Bai
Zhijun
Z
Liu
Licheng
L
Li
Jingjing
J
Wu
Xinwei
X
Sun
Honghe
H
Hu
Tao
T
Yang
Min
M
Wang
Pengzhi
P
Liu
Li
L
Yang
Yinhui
Y
Di
Biao
B
Chen
Weijun
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D017931:DNA Primers; D004196:Disease Outbreaks; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D007223:Infant; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000235:genetics
D017931:DNA Primers; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins