Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.
Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance.
Boltz
Valerie F
VF
HIV Drug Resistance Program, NCI, NIH, Frederick, MD, United States.
Maldarelli
Frank
F
Martinson
Neil
N
Morris
Lynn
L
McIntyre
James A
JA
Gray
Glenda
G
Hopley
Mark J
MJ
Kimura
Toshio
T
Mayers
Douglas L
DL
Robinson
Patrick
P
Mellors
John W
JW
Coffin
John M
JM
Palmer
Sarah E
SE
D000483:Alleles; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics
D017931:DNA Primers; D012367:RNA, Viral