title: Persicarin from water dropwort (Oenanthe javanica) protects primary cultured rat cortical cells from glutamate-induced neurotoxicity.
abstract: The n-BuOH fraction of O. javanica significantly protected the primary cultures of rat cortical cells exposed to glutamate. Four flavonoids yielded from this fraction through bioactivity-guidance. The isolated compounds, identified as isorhamnetin (1), afzelin (2), hyperoside (3) and persicarin (4), were evaluated in vitro for their neuroprotective activity. Persicarin (4), the main constituent of O. javanica, showed significant neuroprotective activities in glutamate-injured rat cortical cells. Persicarin diminished calcium influx and inhibited the subsequent overproduction of nitric oxide and intracellular peroxide. In addition, persicarin significantly restored the reduced activities of glutathione (GSH) reductase and glutathione peroxidase, and the contents of GSH induced by glutamate. These results support a conclusion that persicarin greatly contributes to the neuroprotective activities of O. javanica.
authors: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors: Choong Je
authors: CJ
authors: Ma
authors: Ki Yong
authors: KY
authors: Lee
authors: Eun Ju
authors: EJ
authors: Jeong
authors: Seung Hyun
authors: SH
authors: Kim
authors: Junghyun
authors: J
authors: Park
authors: Yun Hee
authors: YH
authors: Choi
authors: Young Choong
authors: YC
authors: Kim
authors: Sang Hyun
authors: SH
authors: Sung
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid / Q000009:adverse effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis; D031050:Oenanthe / Q000737:chemistry*; D010545:Peroxides / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D047309:Flavones; D005419:Flavonoids; D018696:Neuroprotective Agents; D010545:Peroxides; D010936:Plant Extracts; C551071:persicarin; D009569:Nitric Oxide; D018698:Glutamic Acid; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D002118:Calcium