A theoretical introduction to "combinatory SYBRGreen qPCR screening", a matrix-based approach for the detection of materials derived from genetically modified plants.
The detection of genetically modified (GM) materials in food and feed products is a complex multi-step analytical process invoking screening, identification, and often quantification of the genetically modified organisms (GMO) present in a sample. "Combinatory qPCR SYBRGreen screening" (CoSYPS) is a matrix-based approach for determining the presence of GM plant materials in products. The CoSYPS decision-support system (DSS) interprets the analytical results of SYBRGREEN qPCR analysis based on four values: the C(t)- and T(m) values and the LOD and LOQ for each method. A theoretical explanation of the different concepts applied in CoSYPS analysis is given (GMO Universe, "Prime number tracing", matrix/combinatory approach) and documented using the RoundUp Ready soy GTS40-3-2 as an example. By applying a limited set of SYBRGREEN qPCR methods and through application of a newly developed "prime number"-based algorithm, the nature of subsets of corresponding GMO in a sample can be determined. Together, these analyses provide guidance for semi-quantitative estimation of GMO presence in a food and feed product.
Van den Bulcke
Marc
M
Division of Biosafety and Biotechnology, Scientific Institute of Public Health, J. Wytsmanstreet 14, 1050 Brussels, Belgium. gmolab@sbb.ihe.be
Lievens
Antoon
A
Barbau-Piednoir
Elodie
E
MbongoloMbella
Guillaume
G
Roosens
Nancy
N
Sneyers
Myriam
M
Casi
Amaya Leunda
AL
D000465:Algorithms; D000821:Animal Feed / Q000032:analysis*; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry; D005504:Food Analysis; D030841:Food, Genetically Modified; D008962:Models, Theoretical; D009930:Organic Chemicals / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011804:Quinolines
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I