title: Detection and identification of multiple genetically modified events using DNA insert fingerprinting.
abstract: Current screening and event-specific polymerase chain reaction (PCR) assays for the detection and identification of genetically modified organisms (GMOs) in samples of unknown composition or for the detection of non-regulated GMOs have limitations, and alternative approaches are required. A transgenic DNA fingerprinting methodology using restriction enzyme digestion, adaptor ligation, and nested PCR was developed where individual GMOs are distinguished by the characteristic fingerprint pattern of the fragments generated. The inter-laboratory reproducibility of the amplified fragment sizes using different capillary electrophoresis platforms was compared, and reproducible patterns were obtained with an average difference in fragment size of 2.4 bp. DNA insert fingerprints for 12 different maize events, including two maize hybrids and one soy event, were generated that reflected the composition of the transgenic DNA constructs. Once produced, the fingerprint profiles were added to a database which can be readily exchanged and shared between laboratories. This approach should facilitate the process of GMO identification and characterization.
authors: St-Hyacinthe Laboratory, Canadian Food Inspection Agency (CFIA), St-Hyacinthe, QC J2S 8E3, Canada. philippe.raymond@inspection.gc.ca
authors: Philippe
authors: P
authors: Raymond
authors: Louis
authors: L
authors: Gendron
authors: Moustafa
authors: M
authors: Khalf
authors: Sylvianne
authors: S
authors: Paul
authors: Kim L
authors: KL
authors: Dibley
authors: Somanath
authors: S
authors: Bhat
authors: Vicki R D
authors: VR
authors: Xie
authors: Lina
authors: L
authors: Partis
authors: Marie-Eve
authors: ME
authors: Moreau
authors: Cheryl
authors: C
authors: Dollard
authors: Marie-José
authors: MJ
authors: Coté
authors: Serge
authors: S
authors: Laberge
authors: Kerry R
authors: KR
authors: Emslie
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D013025:Glycine max / Q000737:chemistry / Q000235:genetics*; D003313:Zea mays / Q000737:chemistry / Q000235:genetics*
chemical_list: D018744:DNA, Plant