Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina.
A halophilic archaeon, strain RO1-6(T), was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6(T) were pleomorphic and motile and stained Gram-negative. Strain RO1-6(T) grew well on complex medium and colonies were red-pigmented. It was able to grow at 20-50°C (optimum 37°C), in 2.1-5.1M NaCl (optimum 3.9 M NaCl), in 0.05-0.70 M MgCl₂ (optimum 0.30 M MgCl₂) and at pH 6.5-8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S₂-DGD. The 16S rRNA gene sequence of strain RO1-6(T) showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6(T) was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6(T) is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6(T) (=CGMCC 1.8711(T) =JCM 16054(T)).
Cui
Heng-Lin
HL
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Gao
Xia
X
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Li
Xin-Yi
XY
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Xu
Xue-Wei
XW
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Zhou
Yu-Guang
YG
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Liu
Hong-Can
HC
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhou
Pei-Jin
PJ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D015636:Magnesium Chloride / Q000378:metabolism; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006017:Glycolipids; D010743:Phospholipids; D010860:Pigments, Biological; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride