Methods to enhance laser speckle imaging of high-flow and low-flow vasculature.
The objective of this paper is to present two methods to extend the response range of laser speckle imaging (LSI). We report on the use of two methods (image exposure time control and magnetomotive actuation of exogenous contrast agents) to enhance characterization of high- and low-flow vasculature, respectively. With an exposure time of 10 and 0.01 ms, the linear response range extended to 10 and 280 mm/s, respectively. With application of an AC magnetic field to a solution of stagnant SPIO particles, an apparent increase of approximately 3x in speckle flow index was induced.
Choi
Bernard
B
Department of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, CA 92612, USA. choib@uci.edu
Ringold
Tyson L
TL
Kim
Jeehyun
J
D000465:Algorithms; D001783:Blood Flow Velocity / Q000502:physiology; D003460:Crystallization; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D007834:Lasers; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D019047:Phantoms, Imaging; D012039:Regional Blood Flow / Q000502:physiology; D013997:Time Factors
D005290:Ferric Compounds; C000499:ferric oxide