Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy.
Green tea containing 634 micromol of flavan-3-ols was ingested by human subjects with an ileostomy. Ileal fluid, plasma, and urine collected 0-24 h after ingestion were analysed by HPLC-MS. The ileal fluid contained 70% of the ingested flavan-3-ols in the form of parent compounds (33%) and 23 metabolites (37%). The main metabolites effluxed back into the lumen of the small intestine were O-linked sulphates and methyl-sulphates of (epi)catechin and (epi)gallocatechin. Thus, in subjects with a functioning colon substantial quantities of flavan-3-ols would pass from the small to the large intestine. Plasma contained 16 metabolites, principally methylated, sulphated, and glucuronidated conjugates of (epi)catechin and (epi)gallocatechin, exhibiting 101-256 nM peak plasma concentration and the time to reach peak plasma concentration ranging from 0.8 to 2.2 h. Plasma pharmacokinetic profiles were similar to those obtained with healthy subjects, indicating that flavan-3-ol absorption occurs in the small intestine. Ileostomists had earlier plasma time to reach peak plasma concentration values than subjects with an intact colon, indicating the absence of an ileal brake. Urine contained 18 metabolites of (epi)catechin and (epi)gallocatechin in amounts corresponding to 6.8+/-0.6% of total flavan-3-ol intake. However, excretion of (epi)catechin metabolites was equivalent to 27% of the ingested (-)-epicatechin and (+)-catechin.
Stalmach
Angélique
A
Division of Environmental and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK.
Mullen
William
W
Steiling
Heike
H
Williamson
Gary
G
Lean
Michael E J
ME
Crozier
Alan
A
D000328:Adult; D001682:Biological Availability; D001711:Biotransformation; D002392:Catechin / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D044948:Flavonols / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D005766:Gastrointestinal Contents / Q000737:chemistry; D020719:Glucuronides / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D006207:Half-Life; D006801:Humans; D007081:Ileostomy; D007408:Intestinal Absorption; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*
D044948:Flavonols; D020719:Glucuronides; D013662:Tea; D002392:Catechin; C057580:gallocatechol