title: Methods for preparing fluorescent and neutral red-stained whole mounts of mouse mammary glands.
abstract: Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.
authors: The Lester and Sue Smith Breast Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors: John D
authors: JD
authors: Landua
authors: Adriana P
authors: AP
authors: Visbal
authors: Michael T
authors: MT
authors: Lewis
mesh_terms: D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D008321:Mammary Glands, Animal / Q000033:anatomy & histology*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009499:Neutral Red; D013194:Staining and Labeling; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D009499:Neutral Red