Isolation of common dendritic cell progenitors (CDP) from mouse bone marrow.
In the steady-state lymphoid organ, dendritic cells (DCs) are classified into two major subsets, plasmacytoid DC (pDC) and conventional DC (cDC). A standing question was whether a common progenitor for plasmacytoid and conventional dendritic cells exists during the sequential differentiation from hematopoietic stem cells to dendritic cells. We have recently identified such a common clonogenic plasmacytoid and dendritic cell progenitor (CDP) from mouse bone marrow using antibodies for c-kit, Flt3, and M-CSFR. CDPs generated almost exclusively pDC and cDC in vitro and upon transfer in irradiated and steady-state mice in vivo. Single-cell analysis revealed the existence of clonal progenitors giving rise to both pDC and cDC within the CDP population. Thus, these results prove the existence of a common developmental pathway for at least some pDCs and cDCs in lymphoid organs in vivo.
Onai
Nobuyuki
N
Department of Biodefense Research, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
Manz
Markus G
MG
Schmid
Michael A
MA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism; D020395:Receptors, Interleukin-7 / Q000378:metabolism; D051941:fms-Like Tyrosine Kinase 3 / Q000378:metabolism
D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain; D019009:Proto-Oncogene Proteins c-kit; D016186:Receptor, Macrophage Colony-Stimulating Factor; D051941:fms-Like Tyrosine Kinase 3