[Effect of metabolism activation on the oxidative cell damage induced by cigarette smoking].
OBJECTIVE
To explore the effect of metabolic activation on the oxidative cell damage induced by mainstream smoke (MS).


METHODS
A549 cells were treated with different concentrations of MS for 2 hours in the presence and absence of S9 mix. ROS levels were determined. DNA and chromosome damages were detected. The activities of SOD and GSH-Px were measured.


RESULTS
With different concentrations of MS treatment, the contents of ROS in the cells with S9 mix were significantly higher than those without S9 mix (P<0.05). More serious chromosome and DNA damages in the cells with S9 mix were observed than those without S9 mix. The micronucleus rate, comet rate, L Tail and OTM in the cells with S9 mix were significantly higher than those without S9 mix (P<0.05). With increased dose of MS, the activities of SOD and GSH-Px decreased in both cells with and without S9 mix. But the tendency of decrease in the cells with S9 mix was more obvious than those without S9 mix (P<0.05).


CONCLUSIONS
MS induces oxidative damage in A549 cells and the metabolic activations could increase the oxidative stress and potentiate the cellular genotoxicity.
Wang
Ling
L
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu, China.
Luo
Qing-Ying
QY
Zhang
Li
L
Wu
Mei
M
Zhang
Zun-Zhen
ZZ
D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects; D015162:Micronucleus Tests; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000633:toxicity*
D017382:Reactive Oxygen Species; D012906:Smoke