title: Packed-bed reactor running on babassu oil and glycerol to produce monoglycerides by enzymatic route using immobilized Burkholderia cepacia lipase.
abstract: The aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. The reactor operated continuously for 22 days at 50 degrees C, and during the first 6 days, no significant decrease on the initial lipase activity was observed. Monoglycerides concentration was in the range from 25 to 33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivation profile described by Arrhenius model estimated values of 50 days and 1.37 x 10(-2) h(-1) the half-life and deactivation coefficient, respectively.
authors: Engineering School of Lorena, University of São Paulo, P.O. Box 116-12602-810, Lorena, São Paulo, Brazil.
authors: Larissa
authors: L
authors: de Freitas
authors: Julio C
authors: JC
authors: dos Santos
authors: Gisella Maria
authors: GM
authors: Zanin
authors: Heizir F
authors: HF
authors: de Castro
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D016956:Burkholderia cepacia / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D050178:Monoglycerides; D010938:Plant Oils; D008049:Lipase; D005990:Glycerol