Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions.
The authors demonstrate Raman-resonant imaging based on the simultaneous generation of several nonlinear frequency mixing processes resulting from a 3-color coherent anti-Stokes Raman scattering (CARS) experiment. The interaction of three coincident short-pulsed laser beams simultaneously generates both 2-color (degenerate) CARS and 3-color (non-degenerate) CARS signals, which are collected and characterized spectroscopically - allowing for resonant, doubly-resonant, and non-resonant contrast mechanisms. Images obtained from both 2-color and 3-color CARS signals are compared and found to provide complementary information. The 3-color CARS microscopy scheme provides a versatile multiplexed modality for biological imaging, which may extend the capabilities of label-free non-linear microscopy, e.g. by probing multiple Raman resonances.
Weeks
Tyler
T
NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA 95817, USA.
Schie
Iwan W
IW
Wachsmann-Hogiu
Sebastian
S
Huser
Thomas
T
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000502:physiology; D003116:Color; D003903:Deuterium / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*; D017785:Photons; D011137:Polystyrenes / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014732:Vibration
D011137:Polystyrenes; D003903:Deuterium; D005947:Glucose