Proteome analysis of Aspergillus fumigatus total membrane proteins identifies proteins associated with the glycoconjugates and cell wall biosynthesis using 2D LC-MS/MS.
We attempted to identify membrane proteins associated with the glycoconjugates and cell wall biosynthesis in the total membrane preparations of Aspergillus fumigatus. The total membrane preparations were first run on 1D gels, and then the stained gels were cut and submitted to in-gel digestion followed by 2D LC-MS/MS and database search. A total of 530 proteins were identified with at least two peptides detected with MS/MS spectra. Seventeen integral membrane proteins were involved in N-, O-glycosylation or GPI anchor biosynthesis. Nine membrane proteins were involved in cell wall biosynthesis. Eight proteins were identified as enzymes involved in sphingolipid synthesis. In addition, the proteins involved in cell wall and ergosterol biosynthesis can potentially be used as antifungal drug targets. Our method, for the first time, clearly provided a global view of the membrane proteins associated with glycoconjugates and cell wall biosynthesis in the total membrane proteome of A. fumigatus.
Ouyang
Haomiao
H
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
Luo
Yuanming
Y
Zhang
Lei
L
Li
Yanjie
Y
Jin
Cheng
C
D001232:Aspergillus fumigatus / Q000737:chemistry* / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis*; D006001:Glycoconjugates / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005656:Fungal Proteins; D006001:Glycoconjugates; D008565:Membrane Proteins; D020543:Proteome