title: Mechanism of degradation of immunogenic gluten epitopes from Triticum turgidum L. var. durum by sourdough lactobacilli and fungal proteases.
abstract: As shown by R5 antibody-based sandwich and competitive enzyme-linked immunosorbent assay (ELISA), selected sourdough lactobacilli, in combination with fungal proteases, hydrolyzed gluten (72 h at 37 degrees C) of various cultivars of Triticum turgidum L. var. durum to less than 20 ppm. Complementary electrophoretic, chromatography, and mass spectrometry techniques were used to characterize the gluten and epitope hydrolysis. Nine peptidases were partially purified from the pooled cytoplasmic extract of the sourdough lactobacilli and used to hydrolyze the 33-mer epitope, the most immunogenic peptide generated during digestion of Triticum species. At least three peptidases (general aminopeptidase type N [PepN], X-prolyl dipeptidyl aminopeptidase [PepX], and endopeptidase PepO) were necessary to detoxify the 33-mer without generation of related immunogenic epitopes. After 14 h of incubation, the combination of all or at least six different peptidases totally hydrolyzed the 33-mer (200 mM) into free amino acids. The same results were found for other immunogenic epitopes, such as fragments 57-68 of alpha 9-gliadin, 62-75 of A-gliadin, and 134-153 of gamma-gliadin. When peptidases were used for fermentation of durum wheat semolina, they caused the hydrolysis of gluten to ca. 2 ppm. The in vivo digestion was simulated, and proteins/peptides extracted from pepsin-trypsin (PT) digestion of durum wheat semolina fermented with selected sourdough lactobacilli induced the expression of gamma interferon and interleukin 2 at levels comparable to those of the negative control. Durum wheat semolina fermented with sourdough lactobacilli was freeze-dried and used for making Italian-type pasta. The scores for cooking and sensory properties for this pasta were higher that those of conventional gluten-free pasta.
authors: Department of Plant Protection and Applied Microbiology, University of Bari, 70126 Bari, Italy.
authors: Maria
authors: M
authors: De Angelis
authors: Angela
authors: A
authors: Cassone
authors: Carlo G
authors: CG
authors: Rizzello
authors: Francesca
authors: F
authors: Gagliardi
authors: Fabio
authors: F
authors: Minervini
authors: Maria
authors: M
authors: Calasso
authors: Raffaella
authors: R
authors: Di Cagno
authors: Ruggero
authors: R
authors: Francavilla
authors: Marco
authors: M
authors: Gobbetti
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001706:Biopsy; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004386:Duodenum / Q000473:pathology; D000939:Epitopes / Q000378:metabolism*; D005285:Fermentation; D005511:Food Handling; D005658:Fungi / Q000201:enzymology*; D005983:Glutens / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000502:physiology*; D014908:Triticum / Q000276:immunology*
chemical_list: D016207:Cytokines; D000939:Epitopes; D005983:Glutens; D010447:Peptide Hydrolases