title: Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations.
abstract: The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.
authors: Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Japan. nobuhisa@nagoya-u.jp
authors: Nobuhisa
authors: N
authors: Watanabe
authors: Yozo
authors: Y
authors: Takasaki
authors: Chika
authors: C
authors: Sato
authors: Shoji
authors: S
authors: Ando
authors: Isao
authors: I
authors: Tanaka
mesh_terms: D020134:Catalytic Domain; D002413:Cations, Divalent / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000737:chemistry* / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary
chemical_list: D002413:Cations, Divalent; D004247:DNA; D015247:Deoxyribonuclease HindIII; D008274:Magnesium