title: Angiopoietin balance in septic shock patients treated by direct hemoperfusion with polymyxin b-immobilized fiber.
abstract: Capillary permeability is a tightly regulated feature of microcirculation in all organ beds; however, in sepsis this feature is fundamentally altered. We previously reported elevated levels of vascular endothelial growth factor and its receptor (fms-like tyrosine kinase-1) in patients with septic shock, then investigated two kinds of angiopoietins in those patients. An enzyme-linked immunoassay was used to measure serum angiopoietin-1 and -2 levels in 12 patients with septic shock who were treated by direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX). The angiopoietin-1 level was lower in patients with septic shock (7.01 +/- 10.08 ng/mL) than in controls (28.24 +/- 11.61 ng/mL, P < 0.001), but the angiopoietin-2 level was higher in septic shock patients (40.83 +/- 30.13 ng/mL vs. 2.47 +/- 1.78 ng/mL, P < 0.001). Between seven survivors and five non-survivors there was no significant difference in angiopoietin-1 levels before DHP-PMX therapy. During DHP-PMX therapy, however, the angiopoietin-2 level was significantly decreased in survivors (31.52 +/- 26.15 ng/mL vs. 17.32 +/- 22.46 ng/mL, P = 0.035). Moreover, at the end of the therapy, the angiopoietin-1 level was significantly lower in non-survivors (1.14 +/- 1.30 ng/mL vs. 10.43 +/- 13.56 ng/mL, P = 0.042), but the angiopoietin-2 level in non-survivors was significantly higher (70.79 +/- 40.47 ng/mL vs. 17.32 +/- 22.46 ng/mL, P = 0.019). The angiopoietin-2 level may be associated with vascular permeability in septic patients, and angiopoietins may be suitable markers of disease severity and mortality.
authors: Department of Nephrology, Mito Saiseikai General Hospital, Mito, Ibaraki, Japan. itaruebi@yahoo.co.jp
authors: Itaru
authors: I
authors: Ebihara
authors: Kouichi
authors: K
authors: Hirayama
authors: Kei
authors: K
authors: Nagai
authors: Tomoko
authors: T
authors: Kakita
authors: Yasunori
authors: Y
authors: Miyamoto
authors: Miho
authors: M
authors: Nagai
authors: Yujiro
authors: Y
authors: Ogawa
authors: Shogo
authors: S
authors: Fujita
authors: Homare
authors: H
authors: Shimohata
authors: Hirayasu
authors: H
authors: Kai
authors: Joichi
authors: J
authors: Usui
authors: Kunihiro
authors: K
authors: Yamagata
authors: Masaki
authors: M
authors: Kobayashi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D042683:Angiopoietin-1 / Q000097:blood*; D042702:Angiopoietin-2 / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002199:Capillary Permeability; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006464:Hemoperfusion / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011112:Polymyxin B / Q000737:chemistry; D012720:Severity of Illness Index; D012772:Shock, Septic / Q000401:mortality / Q000503:physiopathology / Q000628:therapy*; D017741:Survivors
chemical_list: C467630:ANGPT1 protein, human; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D015415:Biomarkers; D011112:Polymyxin B