CCR2, MCP-1, SDF-1a & DC-SIGN gene polymorphisms in HIV-1 infected patients with & without tuberculosis.
OBJECTIVE
Variability in the clinical outcome of persons exposed to and infected with HIV-1 and tuberculosis (TB) is determined by multiple factors including host genetic variations. The aim of the present study was to find out whether chemokine, chemokine receptor and DC-SIGN gene polymorphisms were associated with susceptibility or resistance to HIV and HIV-TB in south India.


METHODS
CCR2 V64I (G/A), monocyte chemoattractant protein-1 (MCP-1) -2518 A/G, stromal cell derived factor-1alpha; (SDF-1alpha) 3'UTR G/A and DC-SIGN gene polymorphisms were studied by polymerase chain reaction based methods in HIV-1 infected patients without TB (n=151), with pulmonary TB (PTB) (n=81) and extrapulmonary TB (n=31), 155 PTB patients without HIV and 206 healthy controls.


RESULTS
The genotype frequencies of CCR2 V64I, MCP-1 -2518 and DC-SIGN polymorphisms did not differ significantly between the study groups. A significantly increased frequency of GG genotype of SDF-1alpha polymorphism was observed among HIV+PTB+ patients compared to healthy controls (P=0.009, Pc=0.027).


CONCLUSIONS
Our data suggest that GG genotype of SDF-1alpha 3'UTR polymorphism may be associated with susceptibility to PTB in HIV-1 infected patients. A better understanding of genetic factors that are associated with TB could help target preventive strategies to those HIV patients likely to develop tuberculosis.
Alagarasu
K
K
Tuberculosis Research Centre (ICMR), Chennai, India.
Selvaraj
P
P
Swaminathan
S
S
Raghavan
S
S
Narendran
G
G
Narayanan
P R
PR
D020413:3' Untranslated Regions; D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics*; D018932:Chemokine CCL2 / Q000235:genetics*; D054377:Chemokine CXCL12 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015658:HIV Infections / Q000150:complications / Q000235:genetics; D015497:HIV-1; D006801:Humans; D007194:India; D037181:Lectins, C-Type / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2 / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D016896:Treatment Outcome; D014376:Tuberculosis / Q000209:etiology / Q000235:genetics; D055815:Young Adult; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D020413:3' Untranslated Regions; D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D037181:Lectins, C-Type; D054390:Receptors, CCR2; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C515334:CCR2 protein, human