Controls of dissolved organic matter and chloride on mercury uptake by a marine diatom.
The effects of natural dissolved organic carbon (DOC) from different origins (estuarine, coastal, and diatom decomposed) and chloride (Cl) on the uptake of inorganic mercury [Hg(II)] and methylated mercury (MeHg) by the marine diatom Thalassiosira pseudonana was investigated using radiotracer techniques. We first developed a new method to remove the surface adsorbed mercury and quantified the intracellular mercury uptake by the diatoms. The dominant mercury species (DOC or chloride complexes, based on the mercury speciation phase diagrams) was controlled by the concentrations of DOC and Cl(-), which could explain the effects of DOC and Cl(-) on mercury uptake. DOC complexes dominated Hg(II)'s speciation and reduced its uptake in most seawater examined. DOC complexes dominated MeHg's speciation only at relatively high DOC levels (>100 muM), but it could affect MeHg uptake even when MeHg-Cl complexes dominated. In a mercury-DOC complex dominated system, both the origin and quantity of DOC greatly influenced mercury uptake by the diatoms. Although DOC generally inhibited the uptake of Hg(II) or MeHg, DOC resulting from diatom decomposition enhanced Hg(II) uptake. Under conditions dominated by chloride complexation, neutral mercury chloride species (HgCl(2) or MeHgCl) may control the uptake.
Zhong
Huan
H
Department of Biology, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong.
Wang
Wen-Xiong
WX
D000327:Adsorption / Q000528:radiation effects; D002712:Chlorides / Q000378:metabolism*; D017377:Diatoms / Q000378:metabolism*; D004705:Endocytosis / Q000528:radiation effects; D042541:Intracellular Space / Q000378:metabolism / Q000528:radiation effects; D008628:Mercury / Q000302:isolation & purification / Q000378:metabolism*; D008767:Methylmercury Compounds / Q000302:isolation & purification; D009930:Organic Chemicals / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D012995:Solubility / Q000528:radiation effects; D013499:Surface Properties / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays
D002712:Chlorides; D008767:Methylmercury Compounds; D009930:Organic Chemicals; D008628:Mercury