[Study on effects of baicalin, berberine and Astragalus polysaccharides and their combinative effects on aldose reductase in vitro].
OBJECTIVE
To observe the activities of baicalin, berberine and Astragalus polysaccharides and their combinative effects on aldose reductase (AR) by a screening model of aldose reductase inhibitor (ARI) in vitro.


METHODS
The inhibition of AR by baicalin, berberine and Astragalus polysaccharides and positive drug (Epalrestat) in different concentrations were evaluated, and their combinative effects were studied according to orthogonal t design.


RESULTS
Baicalin and berberine had remarkable inhibitory effects on AR, the inhibitory rates were (88.4 +/- 7.4)% and (69.0 +/- 9.4)% at the concentration of 300 microg/mL. However, the combinative effect of the inhibition on AR by the two compounds was antagonistic action. Astragalus polysaccharides had no activity of inhibition on AR.


CONCLUSIONS
Baicalin and berberine are the potential AR inhibitors as they can inhibit the activity of AR in vitro.
Yang
Hong-Zhou
HZ
Center for Chinese Medical Therapy and Systems Biology, Shanghai University of TCM, Shanghai 211203, China.
Zhou
Ming-Mei
MM
Zhao
Ai-Hua
AH
Xing
Shu-Ning
SN
Fan
Zi-Quan
ZQ
Jia
Wei
W
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D027885:Astragalus propinquus / Q000737:chemistry; D001599:Berberine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D048909:Diabetes Complications / Q000201:enzymology / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship
D004791:Enzyme Inhibitors; D005419:Flavonoids; D011134:Polysaccharides; D001599:Berberine; C038044:baicalin; D000449:Aldehyde Reductase