title: Metabonomic phenotype and identification of "heart blood stasis obstruction pattern" and "qi and yin deficiency pattern" of myocardial ischemia rat models.
abstract: The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up- or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".
authors: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
authors: Bei
authors: B
authors: Yan
authors: JiYe
authors: J
authors: A
authors: HaiPing
authors: H
authors: Hao
authors: GuangJi
authors: G
authors: Wang
authors: XuanXuan
authors: X
authors: Zhu
authors: WeiBin
authors: W
authors: Zha
authors: LinSheng
authors: L
authors: Liu
authors: EnZe
authors: E
authors: Guan
authors: Ying
authors: Y
authors: Zhang
authors: ShengHua
authors: S
authors: Gu
authors: Qing
authors: Q
authors: Huang
authors: YuanTing
authors: Y
authors: Zheng
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D055442:Metabolome / Q000502:physiology; D055432:Metabolomics / Q000379:methods*; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D010641:Phenotype; D019413:Qi; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016710:Yin Deficiency / Q000378:metabolism* / Q000473:pathology
chemical_list: D000596:Amino Acids; D015415:Biomarkers