Genotype of Toxoplasma gondii from blood of stray cats in Gyeonggi-do, Korea.
Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5' and 3' ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.
Kim
Hye-Youn
HY
Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea.
Kim
Yun-Ah
YA
Lee
Ho Sa
HS
Rhie
Ho Gun
HG
Cho
Shin-Hyeong
SH
Yu
Jae-Ran
JR
Lee
Sang-Eun
SE
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001769:Blood / Q000469:parasitology*; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D007723:Korea; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma