[Development of high performance liquid chromatographic fingerprints of liposoluble constituents in donkey-hide glue and tortoise-shell glue].
A sensitive and specific method with high performance liquid chromatographic fingerprints was developed for controlling the quality of donkey-hide glue and tortoise-shell glue according to the liposoluble constituents. The samples were prepared with the liquid-liquid-liquid three-phase static extraction. The liposoluble constituents were analysed by reversed-phase high performance liquid chromatography with a gradient program and detected at 205 nm. The chromatograms were evaluated by similarity and cluster analysis. The common peaks of two glues were marked respectively. The differences between two glues were revealed by similarity and cluster analysis. The method has good repeatability and stability, and can be served as the means to identify different kinds of animal glue.
Yu
Haiying
H
Drug Testing and Analysis Center, School of Pharmacy, Shandong University, Jinan, China.
Zhou
Yongyan
Y
Cheng
Xiumin
X
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000032:analysis / Q000737:chemistry*; D018397:Equidae; D008419:Materia Medica / Q000737:chemistry*; D010449:Peptide Mapping; D012867:Skin / Q000737:chemistry; D014020:Tissue Extracts / Q000737:chemistry; D014426:Turtles
D008419:Materia Medica; D014020:Tissue Extracts; D003094:Collagen