Angustibacter luteus gen. nov., sp. nov., isolated from subarctic forest soil.
An actinobacterial strain was isolated from subarctic forest soil, and subjected to polyphasic taxonomic characterization. The cell-wall peptidoglycan comprised meso-diaminopimelic acid, alanine and glutamic acid. MK-9(H₄) was the predominant isoprenoid quinone, and iso-C₁₇:₀, iso-C₁₅:₀ and C₁₆:₀ were detected as the major cellular fatty acids. Phylogenetic analyses based on 16S rRNA gene sequences showed that this organism was related to members of the suborders Kineosporiineae and Micrococcineae. The phenotypic properties readily differentiated this organism from the phylogenetic neighbours. Based on the phenotypic and genotypic evidence, the strain is assigned to a novel species of a novel genus: Angustibacter luteus gen. nov., sp. nov. with type strain TT07R-79(T) (=NBRC 105387(T) =KACC 14249(T)).
Tamura
Tomohiko
T
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Ishida
Yuumi
Y
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Otoguro
Misa
M
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Yamamura
Hideki
H
Faculty of Engineering, University of the Yamanashi, 4-3-11, Takeda, Kofu, Yamanashi 400-8511, Japan.
Hayakawa
Masayuki
M
Faculty of Engineering, University of the Yamanashi, 4-3-11, Takeda, Kofu, Yamanashi 400-8511, Japan.
Suzuki
Ken-Ichiro
KI
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000409:Alanine / Q000032:analysis; D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D018698:Glutamic Acid; D003960:Diaminopimelic Acid; D000409:Alanine