State of homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma.
Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P<0.05). These results indicate that HOXA10 expression could contribute to cancer progression and prognosis and that HOXA10 may be a potential diagnostic marker and a therapeutic target for OSCCs.
Yamatoji
Masanobu
M
Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
Kasamatsu
Atsushi
A
Yamano
Yukio
Y
Sakuma
Kentaro
K
Ogoshi
Kenji
K
Iyoda
Manabu
M
Shinozuka
Keiji
K
Ogawara
Katsunori
K
Takiguchi
Yuichi
Y
Shiiba
Masashi
M
Tanzawa
Hideki
H
Uzawa
Katsuhiro
K
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; C069634:HOXA10 protein, human