The brome mosaic virus-based recombination vector triggers a limited gene silencing response depending on the orientation of the inserted sequence.
In some RNA viruses (e.g. in brome mosaic virus, BMV), the same factor (intra- or intermolecular hybridization between viral RNA molecules) is capable of inducing two different processes: RNA silencing and RNA recombination. To determine whether there is some interplay between these two phenomena, we have examined if the BMV-based recombination vector containing a plant-genome-derived sequence can function as a gene-silencing vector. Surprisingly, we found that neither dsRNA forming during the replication of the BMV-based vector nor highly structured regions of its genome were effective RNAi triggers. Only mutants carrying a sequence complementary to the target mRNA functioned as gene silencing vectors and were steadily maintained in the infected plant. The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells.
Pacak
Andrzej
A
Department of Gene Expression, Adam Mickiewicz University, Miedzychodzka 5, 60-371, Poznan, Poland.
Strozycki
Pawel M
PM
Barciszewska-Pacak
Maria
M
Alejska
Magdalena
M
Lacomme
Christophe
C
Jarmołowski
Artur
A
Szweykowska-Kulińska
Zofia
Z
Figlerowicz
Marek
M
D017795:Bromovirus / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods*; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D014026:Nicotiana / Q000235:genetics
D012367:RNA, Viral