Rapid screening of antibody aggregates and protein impurities using short gel filtration columns.
Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity, and the multimeric state of a protein of interest. Superdex 200 and Superdex 75 are outstanding GF media for such analysis. To speed up analysis and keep sample and buffer consumption at minimum, two prepacked short GF columns have been developed, Superdex 200 5/150 GL and Superdex 75 5/150 GL. With lengths of only 15 cm and volume of 3 ml, these columns allow rapid analysis (6-12 min/run) with minimal sample (4-50microl) and buffer consumption.
Salomonson
Inger
I
GE Healthcare Bio-Sciences AB, Uppsala, Sweden.
Zou
Jinyu
J
Carlsson
Marianne
M
Bergh
Ann
A
Lundström
Fredrik
F
D000906:Antibodies / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D002021:Buffers; D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000737:chemistry; D013997:Time Factors
D000906:Antibodies; D002021:Buffers; D011506:Proteins