title: Isolation and characterization of lymphatic endothelial cells from human glossal lymphangioma.
abstract: Abnormal lymphangiogenesis is associated with several diseases such as tumor metastasis and lymphangioma. Human lymphangioma originated from the transformation of lymphatic endothelium is a benign malformation of lymphatic vessels and its pathogenesis has up to date not been illuminated and its cell model has also not been established. An optimized method was used to isolate lymphatic endothelial cells from human glossal lymphangioma (GL-LECs) and GL-LECs were further primarily cultured and expanded. GL-LECs were of typical cobblestone appearance when they reached confluence. The weible-palade body was observed in the GL-LECs cytoplasm. Almost all GL-LECs were strongly positive for specific lymphatic markers FLT-4, LYVE-1 and prox-1 by immunocytochemistry. Furthermore, three-dimension tube-like capillaries of GL-LECs resembled the lymphatic system in vivo, and the GL-LECs spheroids sprouted radically out to form three-dimensional buds when embedded in the cultured BME. These results indicated that high purity GL-LECs were successfully isolated and expanded. They had the abilities of tube formation and differentiation in vitro, which provide a favorable cell model for further uncovering the pathogenesis of human lymphangiomas.
authors: Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, P.R. China.
authors: Lanying
authors: L
authors: You
authors: Mingfu
authors: M
authors: Wu
authors: Ying
authors: Y
authors: Chen
authors: Gang
authors: G
authors: Xu
authors: Juncheng
authors: J
authors: Wei
authors: Qiong
authors: Q
authors: Li
authors: Anping
authors: A
authors: Song
authors: Liangpin
authors: L
authors: Zhao
authors: Shuang
authors: S
authors: Li
authors: Zhiqiang
authors: Z
authors: Han
authors: Jianfeng
authors: J
authors: Zhou
authors: Yunpin
authors: Y
authors: Lu
authors: Shixuan
authors: S
authors: Wang
authors: Ding
authors: D
authors: Ma
authors: Li
authors: L
authors: Meng
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D002196:Capillaries / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000473:pathology; D004729:Endothelium, Lymphatic / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008202:Lymphangioma / Q000378:metabolism* / Q000473:pathology*; D009389:Neovascularization, Pathologic; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor