Comparative analysis of the secretory proteome of human adipose stromal vascular fraction cells during adipogenesis.
Adipogenesis is a complex process that is accompanied by a number of molecular events. In this study, a proteomic approach was adopted to identify secretory factors associated with adipogenesis. A label-free shotgun proteomic strategy was implemented to analyze proteins secreted by human adipose stromal vascular fraction cells and differentiated adipocytes. A total of 474 proteins were finally identified and classified according to quantitative changes and statistical significances. Briefly, 177 proteins were significantly upregulated during adipogenesis (Class I), whereas 60 proteins were significantly downregulated (Class II). Changes in the expressions of several proteins were confirmed by quantitative RT-PCR and immunoblotting. One obvious finding based on proteomic data was that the amounts of several extracellular modulators of Wnt and transforming growth factor-beta (TGF-beta) signaling changed during adipogenesis. The expressions of secreted frizzled-related proteins, dickkopf-related proteins, and latent TGF-beta-binding proteins were found to be altered during adipogenesis, which suggests that they participate in the fine regulation of Wnt and TGF-beta signaling. This study provides useful tools and important clues regarding the roles of secretory factors during adipogenic differentiation, and provides information related to obesity and obesity-related metabolic diseases.
Kim
Jaeyoon
J
Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea.
Choi
Yoon Sup
YS
Lim
Seyoung
S
Yea
Kyungmoo
K
Yoon
Jong Hyuk
JH
Jun
Dong-Jae
DJ
Ha
Sang Hoon
SH
Kim
Jung-Wook
JW
Kim
Jae Ho
JH
Suh
Pann-Ghill
PG
Ryu
Sung Ho
SH
Lee
Taehoon G
TG
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002458:Cell Fractionation; D002478:Cells, Cultured; D006801:Humans; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology*
D020543:Proteome