Effect of classic methanogenic inhibitors on the quantity and diversity of archaeal community and the reductive homoacetogenic activity during the process of anaerobic sludge digestion.
In this study, the microbial response of anaerobic sludge digestion to the addition of two classic methanogenic inhibitors (chloroform, 2-bromoethanesulfonate) was investigated. Both the toxicants showed their effectiveness on CH(4) production, whereas the hydrogen responses and acetate accumulations were elicited to different extent. Terminal restriction fragment length polymorphism analyses in combination with clone library showed that both toxicants inhibited not only methanogenic activity but the structure of methanogenic communities. The acetoclastic Methanosaetaceae was more sensitive than hydrogenotrophic Methanobacteriales and Methanomicrobiales. Interestingly, as reflected by the favorable thermodynamic condition and the increase of formyltetrahydrofolate synthetase (fhs) gene copy numbers, reductive homoacetogenesis from H(2)/CO(2) was also stimulated by selective inhibition of methanogenesis with 2-bromoethanesulfonate (BES).
Xu
Kewei
K
Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, People's Republic of China.
Liu
He
H
Chen
Jian
J
D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000693:Anaerobiosis; D001105:Archaea / Q000187:drug effects* / Q000235:genetics; D001483:Base Sequence; D019149:Bioreactors; D002725:Chloroform / Q000633:toxicity*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics; D015723:Gene Library; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D014865:Waste Disposal, Fluid / Q000379:methods*
D017738:Alkanesulfonic Acids; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C018853:2-bromoethanesulfonic acid; D002725:Chloroform; D005574:Formate-Tetrahydrofolate Ligase; D008697:Methane