Dissolved core alginate microspheres as "smart-tattoo" glucose sensors.
The feasibility of multilayer thin film coated dissolved-core alginate-templated microsphere sensors based on fluorescence resonance energy transfer and competitive binding, was explored in simulated interstitial fluid, using glucose as a model analyte. The glucose sensitivity was observed to be 0.89%/mM glucose with a linear response in the range of 0-50 mM glucose. The sensor response was observed to be completely reversible in nature with a response time of 120 seconds. The system was further demonstrated to respond similarly using near-infrared dyes (Alexa Fluor-647-labeled dextran as donor and QSY-21-conjugated apo-GOx as acceptor) which exhibited a sensitivity of 0.94%/mM glucose with a linear response in range of 0-50 mM glucose, making the sensor more amenable to in vivo use, when implanted in scattering tissue.
Chaudhary
Ayesha
A
School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India. ayesha@iitb.ac.in
Raina
Monica
M
McShane
Michael J
MJ
Srivastava
Rohit
R
D000464:Alginates / Q000737:chemistry*; D001698:Biomedical Engineering / Q000379:methods; D015374:Biosensing Techniques; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004867:Equipment Design; D045604:Extracellular Fluid / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008863:Microspheres; D013053:Spectrophotometry / Q000379:methods; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013997:Time Factors
D000464:Alginates; D006603:Hexuronic Acids; C067297:8-aminohexylamino cAMP; D020723:Glucuronic Acid; D000242:Cyclic AMP; D005949:Glucose Oxidase; D005947:Glucose