The physical and genetic framework of the maize B73 genome.
Maize is a major cereal crop and an important model system for basic biological research. Knowledge gained from maize research can also be used to genetically improve its grass relatives such as sorghum, wheat, and rice. The primary objective of the Maize Genome Sequencing Consortium (MGSC) was to generate a reference genome sequence that was integrated with both the physical and genetic maps. Using a previously published integrated genetic and physical map, combined with in-coming maize genomic sequence, new sequence-based genetic markers, and an optical map, we dynamically picked a minimum tiling path (MTP) of 16,910 bacterial artificial chromosome (BAC) and fosmid clones that were used by the MGSC to sequence the maize genome. The final MTP resulted in a significantly improved physical map that reduced the number of contigs from 721 to 435, incorporated a total of 8,315 mapped markers, and ordered and oriented the majority of FPC contigs. The new integrated physical and genetic map covered 2,120 Mb (93%) of the 2,300-Mb genome, of which 405 contigs were anchored to the genetic map, totaling 2,103.4 Mb (99.2% of the 2,120 Mb physical map). More importantly, 336 contigs, comprising 94.0% of the physical map ( approximately 1,993 Mb), were ordered and oriented. Finally we used all available physical, sequence, genetic, and optical data to generate a golden path (AGP) of chromosome-based pseudomolecules, herein referred to as the B73 Reference Genome Sequence version 1 (B73 RefGen_v1).
Wei
Fusheng
F
Arizona Genomics Institute, Department of Plant Sciences, BIO5 Institute for Collaborative Research, University of Arizona, Tucson, Arizona, United States of America.
Zhang
Jianwei
J
Zhou
Shiguo
S
He
Ruifeng
R
Schaeffer
Mary
M
Collura
Kristi
K
Kudrna
David
D
Faga
Ben P
BP
Wissotski
Marina
M
Golser
Wolfgang
W
Rock
Susan M
SM
Graves
Tina A
TA
Fulton
Robert S
RS
Coe
Ed
E
Schnable
Patrick S
PS
Schwartz
David C
DC
Ware
Doreen
D
Clifton
Sandra W
SW
Wilson
Richard K
RK
Wing
Rod A
RA
D000465:Algorithms; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D055620:Optical Phenomena; D020161:Physical Chromosome Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000235:genetics*
D005819:Genetic Markers