Wnt-5a regulates proliferation in lung cancer cells.
Wnt-5a is important in the physiological development and differentiation of lung and is also involved in the regulating proliferation, differentiation and invasion of tumor cells. However, very little is known about the roles of Wnt-5a in the development of lung cancer. The purpose of this study was to explore the role of Wnt-5a in the development of the non-small cell lung cancer, through constructing plasmids containing Wnt-5a sense gene siRNA. We constructed the plasmids containing the Wnt-5a sense gene and siRNA eukaryotic expression vector and transfected it into the human lung squamous carcinoma cell line H157 and adenocarcinoma cell line A549. Expression of Wnt-5a RNA and protein and proliferation of the cells were measured. Expression of Wnt-5a protein significantly stimulated cell proliferation and transfection with siRNA plasmids suppressed Wnt-5 expression and cell proliferation. The human plasmids containing Wnt-5a sense gene and siRNA eukaryotic expression vector was successfully constructed and transfection to human cancer cells induces cell proliferation. siRNA actively suppressed Wnt-5a expression.
Huang
Ying
Y
Department of Respiratory Medicine, The Third Military Medical University, Chongqing, P.R. China. liuguoxiang@162.com
Liu
Guangxiang
G
Zhang
Bo
B
Xu
Gang
G
Xiong
Wei
W
Yang
Heping
H
D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D051153:Wnt Proteins / Q000378:metabolism / Q000502:physiology*; D000071818:Wnt-5a Protein
D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein