title: Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.
abstract: To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
authors: Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea.
authors: JiYoung
authors: J
authors: Hong
authors: Byunghak
authors: B
authors: Kang
authors: Ahyoun
authors: A
authors: Kim
authors: Seoyoun
authors: S
authors: Hwang
authors: Sunwha
authors: S
authors: Lee
authors: Jonghyen
authors: J
authors: Kim
authors: Hyun-Young
authors: HY
authors: Lee
authors: Sang-Hyeon
authors: SH
authors: Kang
authors: Doo-Sung
authors: DS
authors: Cheon
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers