Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells.
This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation.
Tan
Sik-Loo
SL
Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Sulaiman
Sofiah
S
Pingguan-Murphy
Belinda
B
Selvaratnam
L
L
Tai
Cheh-Chin
CC
Kamarul
T
T
D000388:Air; D000650:Amnion / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D020219:Chondrogenesis; D003470:Culture Media / Q000737:chemistry; D005612:Freeze Drying; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D011817:Rabbits
D003470:Culture Media; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors