Evaluation of the genotoxic potential of Austroplenckia populnea (Reiss) Lundell chloroform fraction from barkwood extract in rodent cells in vivo.
The genotoxic effect of the Austroplenckia populnea chloroform fraction from barkwood extract was tested in vivo on peripheral blood cells of Swiss mice with the comet assay (SCGE), and the clastogenic effect was investigated on peripheral blood cells of Swiss mice and bone marrow cells of Wistar rats, with the micronucleus and chromosome aberrations tests. The animals were treated by gavage with 3 concentrations of the extract: 300, 600 and 900 mg.kg-1. Peripheral blood cells of Swiss mice were collected 4 and 24 hours after the treatment to the SCGE assay and 48 and 72 hours to the micronucleus test. Bone marrow cells of Wistar rats were collected 24 hours after the treatment to the micronucleus and chromosome aberration tests. The results showed that the A. populnea chloroform fraction induced an increase in the average number of DNA damage in peripheral blood cells at the three concentrations tested, but this increase was not statistically significant. In the micronucleus and chromosome aberrations test, no significant increase was observed in the mean number of micronucleated polychromatic erythrocytes (MNPCE) of Swiss mice or MNPCE or chromosome aberrations for the rat bone marrow cells, for any of the tested doses. Our findings enable us to conclude that by the comet assay, A. populnea chloroform fraction from barkwood extract showed no genotoxic effects, and by the micronucleus and chromosome aberration tests, the extract fraction showed no clastogenic/aneugenic effects on the rodent cells tested.
Ribeiro
J C
JC
Universidade José do Rosário Vellano-UNIFENAS, Alfenas, MG, Brazil.
Andrade
S F
SF
Bastos
J K
JK
Maistro
E L
EL
D000818:Animals; D029753:Celastraceae / Q000737:chemistry*; D002478:Cells, Cultured; D002725:Chloroform / Q000302:isolation & purification / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D002725:Chloroform