[Determination of five biogenic amines in source water by ion chromatography].
An ion chromatographic (IC) method was developed for the separation of five biogenic amines (BAs), including putrescine, cadaverine, histamine, spermidine and spermine, in source water. The sample was separated on a TSK-GEL SuperIC cation exchange column (150 mm x 4.6 mm) after filtering treatment by 0.45 microm inorganic membrane. Methanesulfonic acid was used as eluent for concentration gradient elution at the rate of 1.0 mL/min. The injection volume was 100 microL and the BAs were detected by a non-suppressed conductivity detector. The result showed that the baseline separation was obtained for the 5 kinds of BAs. The linear relationships between the concentration and the peak area were obtained in the concentration range of 1.0 - 30.0 mg/L. The relative standard deviations (RSDs) of retention time and peak area were no more than 0.02% and 2.08%, respectively. The recoveries were from 96.0% to 107.0%. The method is simple, rapid, highly sensitive and with good repeatability. It could be used for the determination the BAs in the source water.
Zhao
Xinying
X
Beijing Center for Physical and Chemical Analysis, Beijing, China. zhaoxinying_123@sina.com
Jiao
Xia
X
Xia
Min
M
Liu
Qing
Q
Zhang
Jinghua
J
D001679:Biogenic Amines / Q000032:analysis*; D002103:Cadaverine / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005618:Fresh Water / Q000032:analysis*; D006632:Histamine / Q000032:analysis; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
D001679:Biogenic Amines; D014874:Water Pollutants, Chemical; D013096:Spermine; D006632:Histamine; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine