Putrescine-polysaccharide conjugates as transglutaminase substrates and their possible use in producing crosslinked films.
Putrescine (1,4-diaminobutane) was covalently linked to alginate and low-methoxyl pectin to synthesize new aminated polysaccharides. Both putrescine-pectin and -alginate conjugates, although the latter at higher concentrations, were found to be able to act as effective acyl acceptor transglutaminase substrates in vitro using both dimethylated casein and soy flour proteins as acyl donors. Monodansylcadaverine, a well known acyl acceptor transglutaminase substrate, dose-dependently counteracted the covalent binding of the aminated polysaccharides to the proteins. Putrescine-pectin conjugate was also tested to prepare, in combination with soy flour proteins, edible films in the presence of purified microbial transglutaminase. Characterization of the enzymatically crosslinked films showed a significant decreased water vapor permeability, with respect to the ones obtained with non-aminated pectin in the presence of transglutaminase, as well as improved mechanical properties, such as high extensibility. Possible biotechnological applications of hydrocolloid films containing putrescine-polysaccharide derivatives enzymatically crosslinked to proteins were suggested.
Di Pierro
Prospero
P
Department of Food Science, University of Naples "Federico II", Portici, Naples, Italy.
Mariniello
Loredana
L
Sorrentino
Angela
A
Villalonga
Reynaldo
R
Chico
Belkis
B
Porta
Raffaele
R
D000464:Alginates / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D001709:Biotechnology; D003432:Cross-Linking Reagents / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D011700:Putrescine / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity; D011503:Transglutaminases / Q000737:chemistry
D000464:Alginates; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D011134:Polysaccharides; D030262:Soybean Proteins; D010368:Pectins; D020723:Glucuronic Acid; D011503:Transglutaminases; D011700:Putrescine