Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
Goren
Alon
A
Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Ozsolak
Fatih
F
Shoresh
Noam
N
Ku
Manching
M
Adli
Mazhar
M
Hart
Chris
C
Gymrek
Melissa
M
Zuk
Or
O
Regev
Aviv
A
Milos
Patrice M
PM
Bernstein
Bradley E
BE
D000818:Animals; D015982:Bias; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics; D006801:Humans; D051379:Mice; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism
D002843:Chromatin; D014157:Transcription Factors