title: Culture of bovine embryos in intermediate host oviducts with emphasis on the isolated mouse oviduct.
abstract: The oviduct provides the optimal environment for the transport of sperm and oocyte at the earliest stages of mammalian embryo development. During the early postfertilization period, several major developmental events occur in the embryo including (i) the first cleavage division, (ii) activation of the embryonic genome, (iii) compaction of the morula, and (iv) formation of the blastocyst. Most of these events are initiated in the oviduct. The absence of assistance from the oviduct may compromise the developmental ability of the cattle embryo under in vitro culture conditions. The oviducts of several mammalian species, including rabbits, cow, sheep (in situ), and mice (organ culture), can sustain early bovine embryos and yield blastocysts of better quality compared with those of culture conditions in vitro, leading to normal pregnancy rates in recipient animals. This review focuses on the use of oviducts in vitro or in vivo as intermediate hosts for postfertilization culture environment of bovine in vitro-produced zygotes with emphasis on the mouse model.
authors: Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Madrid, Spain. drizos@inia.es
authors: D
authors: D
authors: Rizos
authors: M A
authors: MA
authors: Ramirez
authors: B
authors: B
authors: Pintado
authors: P
authors: P
authors: Lonergan
authors: A
authors: A
authors: Gutierrez-Adan
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary; D047108:Embryonic Development / Q000502:physiology*; D005187:Fallopian Tubes / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D051379:Mice; D011817:Rabbits; D027724:Reproductive Techniques, Assisted / Q000662:veterinary; D012756:Sheep; D013045:Species Specificity