New potent DPPH radical scavengers from a marine-derived actinomycete strain USF-TC31.
Six compounds were isolated as radical scavengers from the culture broth of a marine-derived actinomycete strain USF-TC31. The structures of two novel compounds were determined to be those of N-carbamoyl-2,3-dihydroxybenzamide (5) and 2-acetamido-3-(2,3-dihydroxybenzoylthio)propanoic acid (6), and four known compounds were identified to be anthranilic acid (1), 2,3-dihydroxybenzoic acid (2), 2,3-dihydroxybenzamide (3) and benadrostin (4) on the basis of spectroscopic data. Compound 6 was characterized as a racemate by its specific rotation. Each of the obtained compounds was evaluated for DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, and compounds 2, 3, 5 and 6 each exhibited potent activity in comparison with the butylhydroxytoluene (BHT) positive control.
Sugiyama
Yasumasa
Y
Laboratory of Applied Microbiology, School of Food and Nutritional Sciences, University of Shizuoka, Japan. sugiyasu@u-shizuoka-ken.ac.jp
Hirota
Akira
A
D039903:Actinobacteria / Q000737:chemistry* / Q000378:metabolism; D001713:Biphenyl Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009792:Oceans and Seas; D010851:Picrates / Q000737:chemistry* / Q000302:isolation & purification*
D001713:Biphenyl Compounds; D002396:Catechols; D016166:Free Radical Scavengers; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; C034221:catechol