[Effects of different titanium-treated surfaces on rat's osteoblast function and cell cycle].
This study is designed to compare the distribution, function and cell cycle of rat osteoblast cultured on sandblasting and acid-alkali base titanium surface versus that cultured on carbonated hydroxyapatite titanium surface; the aim is to probe the influences of different surfaces on the function of osteoblasts. SD rat's osteoblasts were primary-cultured, passaged to the 4th generation and then cultured on the two kinds of surfaces. The cellular modality was observed using an inverted microscopy analyzing system. Proliferation was examined by MTT chromatometry and depicted by growth curve. Expression of alkaline phosphatase (ALP) was detected by Enzyme-Linked Immunosorbent Assay. The cell apoptosis and proliferation were examined by flow cytometry. The proliferation curve and index of the osteoblasts on the two kinds of surfaces were almost the same. However, on the carbonated hydroxyapatite group, the level of alkaline phosphatase expression of osteoblasts was higher and kept longer than the other one. In conclusion, the elements on the titanium surface influence the cell function, and the surface with rich PO4(3-) and Ga2+ can enhance the mineralization function of rat osteoblast.
Yang
Xiaoyu
X
Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
Liu
Changhong
C
Liang
Xing
X
Sun
Jun
J
Hong
Caixia
C
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002254:Carbonates / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D006882:Hydroxyapatites / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D002254:Carbonates; D006882:Hydroxyapatites; D014025:Titanium; D000469:Alkaline Phosphatase