Identification of avermectin-high-producing strains by high-throughput screening methods.
Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
Gao
Hong
H
Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.
Liu
Mei
M
Zhou
Xianlong
X
Liu
Jintao
J
Zhuo
Ying
Y
Gou
Zhongxuan
Z
Xu
Bing
B
Zhang
Wenquan
W
Liu
Xiangyang
X
Luo
Aiqun
A
Zheng
Chuansen
C
Chen
Xiaoping
X
Zhang
Lixin
L
D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D057166:High-Throughput Screening Assays; D007218:Industrial Microbiology; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D015203:Reproducibility of Results; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014466:Ultraviolet Rays
D007559:Ivermectin; C019264:avermectin