title: Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha.
abstract: Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1alpha (eEF1alpha) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1alpha and generated a ZPR1 mutant that constitutively interacted with eEF1alpha. ZPR1-DeltaA (Delta193-246) bound to eEF1alpha independently of Zn(2+) in vivo. This study indicates that ZPR1-DeltaA (Delta193-246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1alpha.
authors: Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. yanaka@hiroshima-u.ac.jp
authors: Noriyuki
authors: N
authors: Yanaka
authors: Yurika
authors: Y
authors: Kaseda
authors: Anna
authors: A
authors: Tanaka
authors: Yoshihito
authors: Y
authors: Nogusa
authors: Naoki
authors: N
authors: Sumiyoshi
authors: Norihisa
authors: N
authors: Kato
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D050505:Mutant Proteins; D020648:Peptide Elongation Factor 1; C490987:Zfp259 protein, mouse