Optimal production of 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3.
The hydroxylation of unsaturated fatty acids by bacterial strains is one type of value-adding bioconversion processes. This process generates new hydroxy fatty acids (HFA) carrying special properties such as higher viscosity and reactivity compared with normal fatty acids. Among microbial strains tested for HFA production, Pseudomonas aeruginosa PR3 is well known to utilize various unsaturated fatty acids to produce mono-, di- and tri-hydroxy fatty acids. Previously we reported that strain PR3 could produce a novel value-added hydroxy fatty acid 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid (Bae et al. (2007) Appl. Microbiol. Biotechnol. 75, 435-440). In this study, we focused on the development of the optimal nutritional and environmental conditions for DHD production from palmitoleic acid by PR3. Optimal carbon and nitrogen sources for DHD production were fructose and yeast extract, respectively. Optimal initial medium pH and incubation temperature were pH 8.0 and 30 degrees C and magnesium ion was essentially required for DHD production. Substrate concentration and time of substrate addition were also optimized. Under optimized conditions, maximal DHD production was 1600mg/l representing 26.7% conversion yield.
Bae
Jae-Han
JH
Department of Animal Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
Suh
Min-Jung
MJ
Kim
Beom-Soo
BS
Hou
Ching T
CT
Lee
In-Jung
IJ
Kim
In-Hwan
IH
Kim
Hak-Ryul
HR
D002244:Carbon / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007477:Ions; D008670:Metals / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D010169:Palmitic Acids / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
C553172:7,10-dihydroxy-8-hexadecenoic acid; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D007477:Ions; D008670:Metals; D010169:Palmitic Acids; C008757:palmitoleic acid; C093821:hexadecenoic acid; D002244:Carbon; D009584:Nitrogen