title: [Effect of radiation on the activity and expression of Ca(2+)-Mg(2+)-ATPase in rat masseter muscle].
abstract: OBJECTIVE
  To investigate the activity and expression of Ca(2+)-Mg(2+)-ATPase in irradiated rat masseter muscle.
  
  
  METHODS
  The rats were irradiated locally with a single dose of 20 Gy X-ray. The activities of Ca(2+)-Mg(2+)-ATPase were measured with colorimetric method. The protein expression of Ca(2+)-Mg(2+)-ATPase was determined by Western blotting and immunohistochemistry.
  
  
  RESULTS
  The activities of Ca(2+)-Mg(2+)-ATPase in masseter muscle decreased by approximately 20% and 40% in irradiated rats on days 3 and 30 postirradiation. There was significant difference in the expression of Ca(2+)-Mg(2+)-ATPase protein between irradiated and nonirradiated rats on day 30 postirradiation. Ca(2+)-Mg(2+)-ATPase protein was found in the cytoplasm of masseter muscle.
  
  
  CONCLUSIONS
  The decrease of ATPase activity played an important role in the cause of radiation-induced skeletal muscle injury, while there was no significant reduction in the expression of Ca(2+)-Mg(2+)-ATPase protein in irradiated rat masseter muscle.
authors: Department of Oral Radiology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Zhi-Min
authors: ZM
authors: Li
authors: Xu-Chen
authors: XC
authors: Ma
authors: Xing-Min
authors: XM
authors: Qu
authors: Shou-Ping
authors: SP
authors: Xu
authors: Lin
authors: L
authors: Ma
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D007150:Immunohistochemistry; D008406:Masseter Muscle / Q000201:enzymology / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats
chemical_list: D017301:Ca(2+) Mg(2+)-ATPase