[Genetic diversity of Fritillaria from Sichuan province based on ISSR].
OBJECTIVE
To provide more proofs for expounding the genetic relationships among the (varietal) species in genus Fritillaria from Sichuan province.


METHODS
The ISSR marker technique was used to study relationships and genetic polymorphism of nineteen populations in ten species and one varietal species of genus Fritillaria. Genetic similarities were calculated by using NTSYS software and the dendrogram was constructed by using UPGMA method.


RESULTS
Eleven primers were selected from 35 ISSR primers, and 179 DNA fragments were amplified from 19 populations. Of which, 179 fragments were polymorphic (percentage of polymorphic bands was 86.8%). The genetic similarity among all accessions ranged from 0.569 to 0.855. Clustering analysis showed that the 19 populations of Fritillaria could be distinctively classified into 4 groups. F. cirrhosa, F. przewalskii, F. cirrhosa var. logirnectarea and F. dajitensis were in the first group; The second group was the cluster of F. cirrhosa and F. mellea (wild and cultivated species); The third group was F. sulcisquamosa, F. thunbergii, wabunesis and F. delavayi; F. hupehensis alone formed the fourth group.


CONCLUSIONS
ISSR marker technique is suitable for the genetic diversity of Fritillaria from Sichuan province. Interspecific identifications among the four original species of Bulhus Fritillariae Cirrhosae recorded by pharmacopoeia of China, and between them and the other species of genus Fritillaria from Sichuan province could not be gained by using ISSR markers technique. In addition, the cluster result of genus Fritillaria had some relationships with the geographical distribution.
Li
Kaiqiang
K
College of Agronomy-Sichuan Agricultural Universty, Ya'an 625014, China.
Wu
Wei
W
Zheng
Youliang
Y
Dai
Yong
Y
Xiang
Li
L
Liao
Kai
K
D002681:China; D031410:Fritillaria / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010802:Phylogeny