Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice.
The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.
Bar-On
Liat
L
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Jung
Steffen
S
D000818:Animals; D039521:CD11c Antigen / Q000235:genetics* / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism
D039521:CD11c Antigen; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins