Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease.
Strain KIT 00200-70066-1(T) was isolated from the sputum of a patient with pulmonary disease. Cells of the strain were Gram-variable, facultatively anaerobic, motile, spore-forming rods and formed colourless to white colonies on tryptic soy agar at 30&emsp14;°C and pH&emsp14;7. The pathogenicity of the strain is not known. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, anteiso-C₁₅:₀, iso-C₁₆:₀ and C₁₆:₀ as the major fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids in the polar lipid profile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Paenibacillus, sharing the highest levels of sequence similarity with Paenibacillus nanensis MX2-3(T), Paenibacillus agaridevorans DSM 1355(T) and Paenibacillus alkaliterrae KSL-134(T) (95.4, 95.2 and 94.8 %, respectively), and that it occupied a distinct position within this genus. Combined phylogenetic and phenotypic data supported the conclusion that strain KIT 00200-70066-1(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus sputi sp. nov. is proposed; the type strain is KIT 00200-70066-1(T) (=KCTC 13252(T) =DSM 22699(T)).
Kim
Kwang Kyu
KK
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Lee
Keun Chul
KC
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Yu
Heekyung
H
Korean Institute of Tuberculosis, 14 Woomyun-dong, Socho-gu, Seoul 137-900, Republic of Korea.
Ryoo
Sungweon
S
Korean Institute of Tuberculosis, 14 Woomyun-dong, Socho-gu, Seoul 137-900, Republic of Korea.
Park
Youngkil
Y
Korean Institute of Tuberculosis, 14 Woomyun-dong, Socho-gu, Seoul 137-900, Republic of Korea.
Lee
Jung-Sook
JS
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000145:classification* / Q000302:isolation & purification*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D013183:Sputum / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid