title: [ShRNA of Cyclin D1 decreased the proliferation of human osteosarcoma cell line SOSP-9607.].
abstract: OBJECTIVE
  To investigate the effect of Cyclin D1 shRNA on the apoptosis and proliferation of human osteosarcoma cell line SOSP-9607.
  
  
  METHODS
  Human Cyclin D1 shRNA vector was stably transfected into SOSP-9607 osteosarcoma cells. The mRNA and protein cxpression levels of Cyclin D1 were detected by semiquantitative RT-PCR and Western blot respectively. The cell cycle and pretiferation of osteosarcoma cells were examined by FCM analysis and CCK-8 method, respectively.
  
  
  RESULTS
  After stable transfection of Cyclin D1 shRNA, the expression of Cyclin D1 were inhibited at mRNA and protein levels. The proliferation of SOSP-9607 osteosarcoma cells was inhibited. The difference was significant compared with control groups (P<0.05). At the same time, Cyclin D1 shRNA transfection increased G0/1 phage content and decreased S phage content.
  
  
  CONCLUSIONS
  Cyclin D1 shRNA could down-regulate the expression of Cyclin D1, effectively inhibit the proliferation of osteosarcoma cells, and have significant effect on the cell cycle.
authors: Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Yong
authors: Y
authors: Ding
authors: De-Gang
authors: DG
authors: Fan
authors: Le-Qun
authors: LQ
authors: Shan
authors: Yu-Cai
authors: YC
authors: Wang
authors: Tong-Tao
authors: TT
authors: Yang
authors: Bao-An
authors: BA
authors: Ma
mesh_terms: D001859:Bone Neoplasms; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D012516:Osteosarcoma; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D019938:Cyclin D1