[Construction of recombinant adenovirus containing full-length sense and antisense cyclin B1 cDNA and their impact on proliferation and apoptosis of HeLa cells].
OBJECTIVE
To construct a recombinant adenovirus vector carrying the full-length sense and antisense cyclin B1 cDNA of human, and to investigate the influence of the recombinant adenoviruses on the cultured human cervical carcinoma cell line HeLa.


METHODS
The recombinant adenovirus vectors were constructed by homologous recombination technique. The recombinant adenoviruses were packaged and amplified in 293A cells. Then the recombinant adenoviruses were used to infect HeLa cells. The influence was determined by cell counting and flow cytometry.


RESULTS
The HeLa cells infected with antisense recombinant adenovirus exhibited significant cell apoptosis and growth inhibition compared to the normal HeLa cells and the cells infected with sense recombinant adenovirus.


CONCLUSIONS
Recombinant adenoviruses vectors containing the full-length sense and antisense cDNA of human cyclin B1 is successfully constructed, which has laid a foundation for further studies on its anticancer functions.
Li
Jie
J
Key Laboratory of Bioresources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, China.
Chen
Ping
P
Hu
Huo-Zhen
HZ
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D049109:Cell Proliferation; D056744:Cyclin B1 / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
C534699:CCNB1 protein, human; D056744:Cyclin B1; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins