TP53 mutation profile of esophageal squamous cell carcinomas of patients from Southeastern Brazil.
Esophageal cancer (EC) is among the 10 most common and fatal malignacies in the world, presenting a marked geographic variation in incidence rates between and within different countries. The TP53 tumor suppressor gene is highly mutated in esophageal tumors and its mutation pattern can offer clues to the etiopathology of the tumor. As Brazil presents one of the highest incidence areas in the West, a deeper knowledge of the molecular mechanisms related to EC development in the Brazilian population is needed. We analyzed the mutation profile of 110 esophageal squamous cell carcinomas (ESCC) of patients from Southeastern Brazil (Rio de Janeiro and São Paulo) and collected data regarding alcohol intake and tobacco smoking. We detected 41 mutations in tumor samples from 38 patients. There was no association between mutation frequency and tobacco smoking or alcohol drinking. The most frequently mutated codons were 179, 214, 220 and 248. Codons 179, 220 and 248 are hot-spots for ESCC, but codon 214 presents only 0.7% of the mutations registered in the IARC database. The mutation profile revealed a high percentage of mutations at A:T base pairs (34.1%) followed by deletions (17.1%). We concluded that the mutation profile detected in this study is different from that of patients from Southern Brazil but very similar to that previously seen in French patients, being characterized by a high frequency of mutations at A:T base pairs, which may be associated with acetaldehyde, the metabolic product of ethanol.
Rossini
Ana
A
Departamento de Bioquímica, IBRAG, UERJ, Av. 28 de Setembro, 87 fundos, 4° Andar, CEP: 20551-013, Rio de Janeiro, RJ, Brazil.
de Almeida Simão
Tatiana
T
Marques
Cynthia B
CB
Soares-Lima
Sheila C
SC
Herbster
Suellen
S
Rapozo
Davy Carlos M
DC
Andreollo
Nelson A
NA
Ferreira
Maria A
MA
El-Jaick
Kenya Balbi
KB
Teixeira
Roberto
R
Guimarães
Denise P
DP
Albano
Rodolpho Mattos
RM
Ribeiro Pinto
Luis Felipe
LF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking; D001938:Brazil / Q000453:epidemiology; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012907:Smoking