title: Analysis of mitochondrial pH and ion concentrations.
abstract: Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na(+), and briefly also aspects related to mitochondrial Ca(2+) measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.
authors: Cell Physiology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
authors: Martin
authors: M
authors: vandeVen
authors: Corina
authors: C
authors: Balut
authors: Szilvia
authors: S
authors: Baron
authors: Ilse
authors: I
authors: Smets
authors: Paul
authors: P
authors: Steels
authors: Marcel
authors: M
authors: Ameloot
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002138:Calibration; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D005456:Fluorescent Dyes / Q000378:metabolism; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000378:metabolism; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D057054:Molecular Imaging; D009068:Movement; D012964:Sodium / Q000378:metabolism*; D012984:Software
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012964:Sodium; D002118:Calcium