title: A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking.
abstract: There is a long history of research into body fluid biomarkers in neurodegenerative and neuroinflammatory diseases. However, only a few biomarkers in CSF are being used in clinical practice. One of the most critical factors in CSF biomarker research is the inadequate powering of studies because of the lack of sufficient samples that can be obtained in single-center studies. Therefore, collaboration between investigators is needed to establish large biobanks of well-defined samples. Standardized protocols for biobanking are a prerequisite to ensure that the statistical power gained by increasing the numbers of CSF samples is not compromised by preanalytical factors. Here, a consensus report on recommendations for CSF collection and biobanking is presented, formed by the BioMS-eu network for CSF biomarker research in multiple sclerosis. We focus on CSF collection procedures, preanalytical factors, and high-quality clinical and paraclinical information. The biobanking protocols are applicable for CSF biobanks for research targeting any neurologic disease.
authors: Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands. c.teunissen@vumc.nl
authors: C E
authors: CE
authors: Teunissen
authors: A
authors: A
authors: Petzold
authors: J L
authors: JL
authors: Bennett
authors: F S
authors: FS
authors: Berven
authors: L
authors: L
authors: Brundin
authors: M
authors: M
authors: Comabella
authors: D
authors: D
authors: Franciotta
authors: J L
authors: JL
authors: Frederiksen
authors: J O
authors: JO
authors: Fleming
authors: R
authors: R
authors: Furlan
authors: R Q
authors: RQ
authors: Hintzen
authors: S G
authors: SG
authors: Hughes
authors: M H
authors: MH
authors: Johnson
authors: E
authors: E
authors: Krasulova
authors: J
authors: J
authors: Kuhle
authors: M C
authors: MC
authors: Magnone
authors: C
authors: C
authors: Rajda
authors: K
authors: K
authors: Rejdak
authors: H K
authors: HK
authors: Schmidt
authors: V
authors: V
authors: van Pesch
authors: E
authors: E
authors: Waubant
authors: C
authors: C
authors: Wolf
authors: G
authors: G
authors: Giovannoni
authors: B
authors: B
authors: Hemmer
authors: H
authors: H
authors: Tumani
authors: F
authors: F
authors: Deisenhammer
mesh_terms: D018070:Biological Specimen Banks / Q000592:standards*; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D032921:Consensus; D016206:Databases, Bibliographic / Q000706:statistics & numerical data; D004185:Disability Evaluation; D004739:England; D005500:Follow-Up Studies; D006801:Humans; D008279:Magnetic Resonance Imaging; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000473:pathology; D012720:Severity of Illness Index; D013048:Specimen Handling / Q000379:methods / Q000592:standards*
chemical_list: D015415:Biomarkers