title: Does erythropoietin affect motility of spermatozoa?
abstract: BACKGROUND
  Erythropoietin, which is a hematopoietic growth factor, has been found to play a role in various physiologic processes within the body including testicular steroidogenesis and spermatogenesis. However, it is not known whether erythropoietin is also essential for the normal physiology of mature sperm cells. In this study, the effects of recombinant human erythropoietin beta (rEPO) on sperm motility were investigated.
  
  
  METHODS
  Samples of 37 volunteers (with total motile sperm count>5x10(6)/ml and a total motility of >50% according to WHO criteria) were collected by masturbation following a 3-5 days period of abstinence. After morphometric analysis before and just after washing, samples were either used as control or treated with rEPO at concentrations of 0.1, 1, 10 or 100 mIU/ml, respectively. Control and treated tubes were incubated for 4 h at 37 degrees C.
  
  
  RESULTS
  Total motility, total progressive motility, slow forward and nonmotile sperm counts of 1, 10 and 100 mIU/ml rEPO groups were significantly improved. This effect was dose independent.
  
  
  CONCLUSIONS
  No significant effect was found at 0.1 mIU/ml concentration. These results suggest that supplementation of media used for sperm preparation techniques with erythropoietin might be beneficial. Further studies are needed to clarify the mechanism of action of erythropoietin on mature sperm cells.
authors: Obstetrics and Gynecology Department, Zeynep Kamil Hospital, and Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey. niyazitug@hotmail.com
authors: Niyazi
authors: N
authors: Tug
authors: Muhammed Eyup
authors: ME
authors: Altunkaynak
authors: Ranan Gulhan
authors: RG
authors: Aktas
authors: Ulkan
authors: U
authors: Kilic
authors: Bayram
authors: B
authors: Yilmaz
authors: Cetin
authors: C
authors: Cam
authors: Ates
authors: A
authors: Karateke
mesh_terms: D000328:Adult; D004921:Erythropoietin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010865:Pilot Projects; D011994:Recombinant Proteins; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin