Fertilizability, developmental competence, and chromosomal integrity of oocytes microinjected with pre-treated spermatozoa in mice.
The aim of the present study was to investigate the safety of sperm pre-treatment during the ICSI procedure using a mouse model. Mouse spermatozoa were treated with methyl-beta-cyclodextrin, lysolecithin, Triton X-100, and dithiothreitol (DTT), and injected into mouse oocytes. The injected oocytes were monitored for chromosomal integrity and pre- and post-implantation development. The chromosomal integrity of the injected oocytes was impaired by in vitro incubation and chemical antagonism. Particularly in the 60-min DTT group, severe chromosome damage increased. Despite the chromosomal damage, the resultant embryos frequently developed to the blastocyst stage. However, the embryos in the 60-min DTT group had significantly higher chromosomal damage and decreased developmental competence to live fetuses. These results indicate that excessive sperm pre-treatment such as DTT for 60 min generates severe chromosome damage in injected oocytes, and that the damage decreases developmental competence to live fetuses but not to blastocysts.
Watanabe
Hiroyuki
H
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Suzuki
Hiroshi
H
Fukui
Yutaka
Y
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D004229:Dithiothreitol / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects / Q000379:methods; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008845:Microinjections; D017830:Octoxynol / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D013048:Specimen Handling / Q000379:methods; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000494:pharmacology
D008244:Lysophosphatidylcholines; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D017830:Octoxynol; D004229:Dithiothreitol