Derivatives of thiocolchicine and its applications to CEM cells treatment using 19F magnetic resonance ex vivo.
It was shown, that cultured ex vivo human T-Lymphoblastoid (CEM) cells respond to synthesized thiocolchicine and fluorine thiocolchicine derivatives. The preparation of derivatives with substitution at C-3 and C-7 is described. All compounds were used at concentration from 1 nM to 1000 nM. Inhibitory effects of these compounds were examined in the three-dimensional (3-D) culture and cells morphology during treatment was monitored using 9.4 T MRI system. We performed studies of these compounds in CEM cells ex vivo using 1H and 19F Magnetic Resonance Imaging (MRI), 19F Magnetic Resonance Spectroscopy (MRS), High Performance Liquid Chromatography coupled with Ultra Violet (HPLC-UV) and Electron Impact Mass Spectrometry (EIMS). The results of the multi-technique approach are consistent with the fact that the new derivatives are more efficient than colchicine and thiocolchicine ex vivo.
Bartusik
Dorota
D
National Research Council Canada, Institute for Biodiagnostics (West), 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1. dorota.bartusik@gmail.com
Tomanek
Boguslaw
B
Lattová
Erika
E
Perreault
Hélène
H
Tuszynski
Jack
J
Fallone
Gino
G
D019149:Bioreactors; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D005461:Fluorine / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D005461:Fluorine; C014856:thiocholchicine; D003078:Colchicine