title: GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.
abstract: The lacto-series gangliosides 3'-isoLM1 and 3',6'-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3'-isoLM1 and 3',6'-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene beta3Gn-T5 knockout mice. In this study, we immunized beta3Gn-T5 knockout mice with 3'-isoLM1/3',6'-isoLD1 and produced the anti-3'-isoLM1/3',6'-isoLD1 mAb GMab-1, of the IgG(3) subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.
authors: Department of Pathology, Duke University Medical Center, DUMC-3156, Durham, NC 27710, USA.
authors: Yukinari
authors: Y
authors: Kato
authors: Chien-Tsun
authors: CT
authors: Kuan
authors: Jinli
authors: J
authors: Chang
authors: Mika Kato
authors: MK
authors: Kaneko
authors: Joanne
authors: J
authors: Ayriss
authors: Hailan
authors: H
authors: Piao
authors: Vidyalakshmi
authors: V
authors: Chandramohan
authors: Charles
authors: C
authors: Pegram
authors: Roger E
authors: RE
authors: McLendon
authors: Pam
authors: P
authors: Fredman
authors: Jan-Eric
authors: JE
authors: Månsson
authors: Darell D
authors: DD
authors: Bigner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D005910:Glioma / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005732:Gangliosides; D007074:Immunoglobulin G; C078813:IV(3)-NeuAc-LcOse4Cer; C078812:IV(3)-NeuAc-III(6)-NeuAc-LcOse4Cer; D017351:N-Acetylglucosaminyltransferases; C067641:lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase