Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa.
CONCLUSIONS
Collagen deposition was increased in regenerative rabbit maxillary mucosa, and the elevated collagen deposition during wound healing was associated with increased interleukin (IL)-6 and IL-8, and decreased IL-10.


OBJECTIVE
To observe the natural progression of wound healing in rabbit maxillary sinus mucosa and to detect the expression of relevant cytokines.


METHODS
A rabbit wound-healing model was established in the maxillary sinus. At different times after injury, the sizes of maxillary ostia were recorded, and histological changes in the sinus mucosa were examined by means of hematoxylin and eosin (H&E) and Masson trichrome staining. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentrations of IL-6, IL-8, IL-10, and collagen types I and III.


RESULTS
A large amount of immature spindled collagen deposition was observed in the basal lamina and lamina propria of regenerative mucosa. IL-6 was increased significantly in regenerative mucosa after injury, especially at 14 days post wounding when compared with normal mucosa. IL-8 was also increased significantly in regenerative mucosa; however, IL-10 was decreased significantly when compared with normal mucosa. IL-6 and IL-8 showed a significant correlation with the expression level of collagen type I.
Sun
Xicai
X
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Wang
Dehui
D
Yu
Huapeng
H
Hu
Li
L
D000818:Animals; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007378:Interleukins / Q000378:metabolism*; D008443:Maxillary Sinus / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D009092:Mucous Membrane / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D010254:Paranasal Sinus Diseases / Q000473:pathology; D011817:Rabbits; D014945:Wound Healing
D024042:Collagen Type I; D024061:Collagen Type III; D007378:Interleukins