[The value of regulated upon activation normal T cell expressed and secreted in the pathogenesis of varicose ulcer of lower limbs].
OBJECTIVE
To study the effectiveness of RANTES in the pathogenesis of venous ulceration.


METHODS
40 Chronic venous insufficiency (CVI) individuals were enrolled in the study and separated into the ulceration group (n=20) and non-ulceration group (n=20), according to CVI with or without ulceration, 10 healthy individuals were enrolled as control group. The expression of RANTES mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in blood. Immunohistochemical staining of RANTES were carried in normal skin, in the tissue at the brim of ulceration and in the tissue of cured or curing ulceration.


RESULTS
In ulceration group, the expression of RANTES mRNA in blood increased obviously. Compared with the other two groups, they were significant different (P < 0.05). The difference was also significance in the other two groups (P < 0.05). The average number of RANTES positive expression in normal skin is 28 +/- 13, in the tissue at the brim of ulceration is 107 +/- 44, and in the tissue of cured or curing ulceration is 35 +/- 20. There are significant difference between normal skin and the tissue at the brim of ulceration (P < 0.05). The average number of RANTES positive expression in the tissue of cured or curing ulceration is lower than that's in the tissue at the brim of ulceration.


CONCLUSIONS
The high expression of RANTES may be one of the important mechanisms of varicose ulcer.
Chen
Liu-hu
LH
Vascular Surgery Center of Sun Yat-sen University, Department of Vascular Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Liu
Da-yue
DY
Xiong
Jiang
J
Ye
Cai-sheng
CS
Yin
Heng-hui
HH
Wang
Shen-ming
SM
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000378:metabolism*; D005260:Female; D006801:Humans; D035002:Lower Extremity / Q000098:blood supply; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D014647:Varicose Ulcer / Q000378:metabolism* / Q000473:pathology; D014689:Venous Insufficiency / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D018946:Chemokine CCL5; D012333:RNA, Messenger