title: Animal study for airway inflammation triggered by gastroesophageal reflux.
abstract: BACKGROUND
  Gastroesophageal reflux disease with extra-esophageal symptoms, especially those with respiratory distress was attracting more and more attention. The related mechanisms were still in controversy. The purpose of the work was to explore airway inflammation triggered by gastroesophageal reflux.
  
  
  METHODS
  Sixteen Sprague-Dawley rats were used as study group and 9 as control. In the study group, a plastic extender with a trumpet-shaped distal end was inserted into the lower esophagus to dilate the cardia, the pylorus was ligated. One ml of 0.1 mol/L hydrochloric acid was injected into the stomach. While a simple laparotomy was performed for control animals. All animals from two groups were sacrificed 24 hours after operation. Then tracheotomy was carried and the bronchoalveolar lavage fluid was collected in all animals. Cells in the fluid were counted and levels of interleukin (IL)-5, -6, -8 in it were measured.
  
  
  RESULTS
  Compared with control group, the study group presented a neutrophil pattern of airway inflammation and an elevated concentration of IL-5, -6, -8 with no significant difference regarding eosinophil count.
  
  
  CONCLUSIONS
  The gastroesophageal reflux-triggered airway inflammation is characterized by a neutrophilic airway inflammation which differed from that caused by asthma, and enhanced levels of IL-5, -6 and -8, which are similar to that caused by asthma.
authors: Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China.
authors: Yun-gang
authors: YG
authors: Lai
authors: Zhong-gao
authors: ZG
authors: Wang
authors: Feng
authors: F
authors: Ji
authors: Ji-min
authors: JM
authors: Wu
authors: Xiu
authors: X
authors: Chen
authors: Zhen
authors: Z
authors: Li
authors: Shu-kui
authors: SK
authors: Dong
mesh_terms: D000818:Animals; D001249:Asthma / Q000209:etiology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005764:Gastroesophageal Reflux / Q000150:complications*; D007249:Inflammation / Q000209:etiology*; D015848:Interleukin-5 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015848:Interleukin-5; D015850:Interleukin-6; D016209:Interleukin-8