title: [The correlation between dendritic cell-specific ICAM-3 grabbing nonintegrin expression and Mycobacterium tuberculosis in humans].
abstract: OBJECTIVE
  To investigate the correlation between dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) expression and Mycobacterium tuberculosis in humans.
  
  
  METHODS
  The peripheral blood mononuclear cells were obtained respectively from 25 patients with tuberculosis, 25 patients with pneumonia and 25 healthy individuals, and were cultured in medium with GM-CSF and IL-4. Five days later, expression of CD(11c), CD(86), HLA-DR and DC-SIGN were detected by flow cytometry. The expression of DC-SIGN mRNA was detected by RT-PCR. The levels of IL-12 and IL-10 in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Proliferation induction of T lymphocytes dendritic cells (DCs) was measured by mixed lymphocyte reaction (MLR).
  
  
  RESULTS
  The expression of CD(86) on DCs in the tuberculosis patients [(72 +/- 11)%] was significantly lower than those in the other 2 groups [(87 +/- 16)%, (92 +/- 6)%] (F = 11.97, P < 0.01). In patients with tuberculosis, the expression of DC-SIGN on DCs [(85 +/- 8)%] was significantly higher than the other 2 groups [(60 +/- 28)%, (62 +/- 13)%] (F = 8.27, P < 0.01), and so was the DC-SIGN mRNA level (F = 3.99, P < 0.05). The levels of IL-10 secreted by DCs in the tuberculosis patients [(98 +/- 31) ng/L] were significantly higher than those in the other 2 groups [(74 +/- 38) ng/L and (66 +/- 27) ng/L] (F = 4.19, P < 0.05). MLR showed lower potency of proliferation induction of T lymphocytes in the tuberculosis patients (1858 +/- 628) than the other 2 groups (3066 +/- 1389), (3383 +/- 1163) (F = 7.92, P < 0.01). Expression of CD(11c) and HLA-DR on DCs and IL-12 levels were not significantly different among the 3 groups (P > 0.05).
  
  
  CONCLUSIONS
  The decreased DC maturity, elevated IL-10 secretion by DCs, decreased proliferation induction of T lymphocytes and impaired immune response may be due to overexpression of DC-SIGN in patients with tuberculosis.
authors: Department of Respiratory Medicine, First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Jie
authors: J
authors: Zhang
authors: Shu-liang
authors: SL
authors: Guo
authors: Yong-ai
authors: YA
authors: Luo
mesh_terms: D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D016753:Interleukin-10; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C508609:IL10 protein, human