[Affects of different bioreactors in process of Salvia miltiorrhiza adventitious root culture].
OBJECTIVE
To study the affects of the different bioreactors on the Salvia miltiorrhiza adventitious root culture.


METHODS
Adventitious roots of S. miltiorrhiza were induced and in vitro cultured in bioreactors. The type of bioreactors was optimized and the kinetics of root growth, accumulation of the active ingredients was also investigated by HPLC.


RESULTS
It showed that the 3-1 conical bubble bioreactor (CNBB) with 60 degrees taper favors achieved the highest active compounds accumulation in adventitious roots. The growth curve and secondary metabolites accumulation curve looks like "S" in CNBB bioreactor. The maximum adventitious roots biomass of 16.24 g x L(-1) (fresh weight) was obtained at 35 day. The highest content of tanshinone IIA (TA) and protocatechuic aldehyde (PA) reached 0.23 mg x g(-1) DW and 0.51 mg x g(-1) DW at 40 day, respectively.


CONCLUSIONS
The 3-1 conical bubble bioreactor (CNBB) with 60 degrees taper favors was the best bioreactors for the accumulation of active compounds.
Yan
Shuo
S
Tianjin University School of Pharmaceutical Science and Biotechnology, Tianjin 300072, China.
Gao
Wenyuan
W
Wang
Juan
J
Huang
Tao
T
Cao
Yu
Y
D018533:Biomass; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010936:Plant Extracts