PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction.
This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4 degrees C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious.
Buathong
Saiwasan
S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Mungthin
Mathirut
M
Tan-ariya
Peerapan
P
Naaglor
Tawee
T
Taamasri
Paanjit
P
Suwannahitatorn
Picha
P
Leelayoova
Saovanee
S
D000818:Animals; D003080:Cold Temperature; D018509:DNA, Helminth / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012965:Sodium Chloride; D013997:Time Factors
D018509:DNA, Helminth; D012965:Sodium Chloride