title: Poxvirus proteomics and virus-host protein interactions.
abstract: Studies of the functional proteins encoded by the poxvirus genome provide information about the composition of the virus as well as individual virus-virus protein and virus-host protein interactions, which provides insight into viral pathogenesis and drug discovery. Widely used proteomic techniques to identify and characterize specific protein-protein interactions include yeast two-hybrid studies and coimmunoprecipitations. Recently, various mass spectrometry techniques have been employed to identify viral protein components of larger complexes. These methods, combined with structural studies, can provide new information about the putative functions of viral proteins as well as insights into virus-host interaction dynamics. For viral proteins of unknown function, identification of either viral or host binding partners provides clues about their putative function. In this review, we discuss poxvirus proteomics, including the use of proteomic methodologies to identify viral components and virus-host protein interactions. High-throughput global protein expression studies using protein chip technology as well as new methods for validating putative protein-protein interactions are also discussed.
authors: Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Box 100266, Gainesville, FL 32610, USA.
authors: Kim
authors: K
authors: Van Vliet
authors: Mohamed R
authors: MR
authors: Mohamed
authors: Leiliang
authors: L
authors: Zhang
authors: Nancy Yaneth
authors: NY
authors: Villa
authors: Steven J
authors: SJ
authors: Werden
authors: Jia
authors: J
authors: Liu
authors: Grant
authors: G
authors: McFadden
mesh_terms: D000818:Animals; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D011212:Poxviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011213:Poxviridae Infections / Q000821:virology*; D040901:Proteomics; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins