Pueraria thunbergiana inhibits cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals.
The radix of Pueraria thunbergiana (P. thunbergiana) is traditionally prescribed to attenuate the clinical manifestation of inner ear dysfunction and various clinical situations including fevers, gastrointestinal disorders, skin problems, migraine headaches, lowering cholesterol, and treating chronic alcoholism in oriental medicine. In the present study, we examined the protective effect of ethanol extract of the radix of P. thunbergiana (RPT) on cisplatin-induced damage of HEI-OC1 auditory hair cells. When the cells were cultured in the medium containing 5-100 microg/mL of RPT, RPT showed protective effect against the cisplatin-induced HEI-OC1 cell damage. We also measured the effects of RPT on lipid peroxidation of cisplatin-treated cells as well as scavenging activities against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. RPT reduced cisplatin-induced lipid peroxidation in a dose-dependent manner. Furthermore, RPT showed strong scavenging activity against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that RPT protects cisplatin-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and scavenging activities of free radials.
Yu
Hyeon-Hee
HH
Department of Food and Nutrition, Kunsan National University, Kunsan, South Korea.
Jung
Su-Young
SY
Shin
Mee-Kyung
MK
Park
Raekil
R
So
Hong-Seob
HS
You
Yong-Ouk
YO
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000009:adverse effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000494:pharmacology; D006198:Hair Cells, Auditory / Q000187:drug effects*; D015227:Lipid Peroxidation; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*
D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D002945:Cisplatin