title: Determination of the diversity of Rhodopirellula isolates from European seas by multilocus sequence analysis.
abstract: In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species.
authors: Department of Microbiology, Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany.
authors: Nadine
authors: N
authors: Winkelmann
authors: Ulrike
authors: U
authors: Jaekel
authors: Carolin
authors: C
authors: Meyer
authors: Wilbert
authors: W
authors: Serrano
authors: Reinhard
authors: R
authors: Rachel
authors: Ramon
authors: R
authors: Rosselló-Mora
authors: Jens
authors: J
authors: Harder
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D005787:Gene Frequency; D005798:Genes, Bacterial; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D019015:Geologic Sediments; D008969:Molecular Sequence Data; D009659:North Sea; D009792:Oceans and Seas; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S