title: Outbreak of Candida parapsilosis in a neonatal intensive care unit: a health care workers source.
abstract: Nosocomial neonatal candidiasis is a major problem in infants, which require intensive therapy. The subjects of the present study were three preterm infants admitted to the neonatal intensive care unit of the General Hospital "Dr. Manuel Gea Gonzalez". The infants developed Candida parapsilosis infection on the mean age of 13.6 day of life. Prior to fungemia, infants had received assisted ventilation and hyperalimentation through central venous catheter. Sequence analysis of the internal transcribed spacer gene ruled out other Candida species and revealed that the eight isolates were C. parapsilosis. The isolates were examined based on their molecular relation by random amplified polymorphic DNA analysis. The profiles allowed the identification of two main genotypes of C. parapsilosis as the outbreak cause and as a result of the cross-infection with health care workers' hands. We conclude that C. parapsilosis commonly colonize through horizontal transmission due to the staff's noncompliance of hand hygiene procedures.
authors: Dirección de Investigación, Hospital General Dr. Manuel Gea González, Tlalpan, 14080, Mexico. rhc@servidor.unam.mx
authors: Rigoberto
authors: R
authors: Hernández-Castro
authors: Sara
authors: S
authors: Arroyo-Escalante
authors: Erika M
authors: EM
authors: Carrillo-Casas
authors: David
authors: D
authors: Moncada-Barrón
authors: Elizabeth
authors: E
authors: Alvarez-Verona
authors: Lorena
authors: L
authors: Hernández-Delgado
authors: Patricia
authors: P
authors: Torres-Narváez
authors: Antonio
authors: A
authors: Lavalle-Villalobos
mesh_terms: D002177:Candidiasis / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology* / Q000517:prevention & control; D055499:Catheter-Related Infections / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology* / Q000517:prevention & control; D002405:Catheterization, Central Venous / Q000009:adverse effects; D003428:Cross Infection / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology* / Q000517:prevention & control; D004196:Disease Outbreaks; D004200:Diseases in Twins / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology* / Q000517:prevention & control; D004866:Equipment Contamination / Q000517:prevention & control*; D016469:Fungemia / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology* / Q000517:prevention & control; D006235:Hand Disinfection; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007363:Intensive Care Units, Neonatal; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D019105:Random Amplified Polymorphic DNA Technique