Effect of human cytomegalovirus infection on nerve growth factor expression in human glioma U251 cells.
OBJECTIVE
To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV).


METHODS
U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV.


RESULTS
The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P < 0.05).


CONCLUSIONS
HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
Wang
Hai-Tao
HT
Department of Microbiology, Qingdao Medical College, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China.
Wang
Bin
B
Liu
Zhi-Jun
ZJ
Bai
Zhi-Qiang
ZQ
Li
Ling
L
Liu
Hai-Yan
HY
Qian
Dong-Meng
DM
Yan
Zhi-Yong
ZY
Song
Xu-Xia
XX
D000199:Actins / Q000378:metabolism; D045744:Cell Line, Tumor; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000150:complications* / Q000235:genetics / Q000473:pathology / Q000821:virology*; D006801:Humans; D008954:Models, Biological; D020932:Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000199:Actins; D012333:RNA, Messenger; D020932:Nerve Growth Factor