Positive influence of hyaluronate on cell vitality of human conjunctival cells after alkali injuries.
BACKGROUND
To analyse the influence of hyaluronate (HA) on the cell vitality of human conjunctival cells after chemical injuries from NaOH or alkali-containing household cleaners.


METHODS
Human conjunctival cells (Chang cells) were incubated either with NaOH solutions or dilutions of household cleaners. HA (0.025%) was added to the cell culture medium of part of the cells subsequent to chemical injuring. After 24 h the cell vitality of the HA-incubated cells, determined by MTS assay (Cell Titer 96(R) Aqueous One Solution Cell Proliferation Assay), was compared to the control cells without HA addition.


RESULTS
The addition of HA to the cell culture medium subsequent to chemical injuring resulted in a significantly lower decrease in cell vitality (mean difference: 11.79; p = 0.00; CI = 98%).


CONCLUSIONS
Because HA attenuates the decrease in cell vitality of alkali-damaged human conjunctival cells, formulations containing HA may be recommended for the treatment of chemical injuries to the anterior part of the eye.
Rabensteiner
Dieter Franz
DF
Department of Ophthalmology, Medical University of Graz, Graz, Austria. df@rabensteiner.cc
Trummer
Gabriele
G
Spitzenberger
Helga
H
Faschinger
Christoph
C
Schmut
Otto
O
Wedrich
Andreas
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000468:Alkalies / Q000633:toxicity*; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology* / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D018709:Statistics, Nonparametric
D000276:Adjuvants, Immunologic; D000468:Alkalies; D006820:Hyaluronic Acid