The use of COS-1 cells for studies of field and laboratory African swine fever virus samples.
Different naturally occurring, cell adapted or genetically manipulated stocks of African swine fever virus were able to infect directly cultures of COS-1 cells, producing extensive cytopathic effects and amounts from 10(6) to 10(7) of infective progeny virus per ml. The induction of late virus-specific proteins, demonstrated by RT-PCR and immunoblotting, and the development of lysis plaques by all the virus samples tested so far, allowed the optimization of both titration and diagnostic assays, as well as the proposal of a method for selection of virus clones during the generation of virus mutants with specific gene deletions.
Hurtado
Carolina
C
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, c/Nicolás Cabrera no 1, 28049 Madrid, Spain.
Bustos
María José
MJ
Carrascosa
Angel L
AL
D000358:African Swine Fever Virus / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D020869:Gene Expression Profiling; D015151:Immunoblotting; D020133:Reverse Transcriptase Polymerase Chain Reaction; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis
D014764:Viral Proteins