Titanates deliver metal ions to human monocytes.
Amorphous peroxotitantes (APT) are insoluble titanium-based particles that bind a variety of metal compounds with high affinity; these particles could be sequestered locally in a solid phase to deliver metal-based drugs. Previous studies have confirmed the 'biodelivery' of metals from metal-APT complexes to fibroblasts, but not monocytes. Our goal in the current study was to use monocytic cytokine secretion to assess delivery of gold or platinum-based compounds from APT to human THP1 monocytes. Cytokine secretion was not triggered by APT alone or metal-APT complexes. In monocytes activated by lipopolysaccharide (LPS), APT alone enhanced or suppressed IL1beta or IL6 secretion, yet TNFalpha secretion was unaffected. Complexes of APT and Au(III) or cis-platin altered LPS-activated IL6 or IL1beta secretion most, TNFalpha least. Our results suggest that the APT deliver metals to monocytes.
Wataha
John C
JC
Department of Restorative Dentistry, University of Washington School of Dentistry, Seattle, WA 98197-7456, USA. jwataha@gmail.com
Hobbs
David T
DT
Wong
Jacqueline J
JJ
Dogan
Sami
S
Zhang
Hai
H
Chung
K-H
KH
Elvington
Mark C
MC
D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000494:pharmacology; D002478:Cells, Cultured; D002945:Cisplatin / Q000008:administration & dosage / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006046:Gold / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007477:Ions / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008422:Materials Testing; D008670:Metals / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010087:Oxides / Q000493:pharmacokinetics / Q000494:pharmacology; D014025:Titanium / Q000493:pharmacokinetics* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001672:Biocompatible Materials; D004337:Drug Carriers; D015850:Interleukin-6; D007477:Ions; D008070:Lipopolysaccharides; D008670:Metals; D010087:Oxides; D014409:Tumor Necrosis Factor-alpha; C525208:peroxo-titanate; D006046:Gold; D014025:Titanium; D002945:Cisplatin