title: Elucidation of genes relevant to the microaerobic growth of Corynebacterium glutamicum.
abstract: Mutagenized cell libraries of Corynebacterium glutamicum were screened for mutants that lost the ability to grow under low oxygen concentrations. The resulting high-oxygen-requiring mutants were used to clone wild-type DNA fragments that could complement the phenotype. Sequencing and subcloning analyses identified six genes, Cgl0807, Cgl1102, Cgl0600, Cgl1427, Cgl2857, and Cgl2859, as the genes responsible for complementation. Some of these genes showed cross-complementation of the mutants in oxygen-limiting static culture, suggesting the utility of these genes for improved growth and production under oxygen limitation.
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano, Japan. m_ikeda@shinshu-u.ac.jp
authors: Masato
authors: M
authors: Ikeda
authors: Masahiro
authors: M
authors: Baba
authors: Nobuyoshi
authors: N
authors: Tsukamoto
authors: Tomoha
authors: T
authors: Komatsu
authors: Satoshi
authors: S
authors: Mitsuhashi
authors: Seiki
authors: S
authors: Takeno
mesh_terms: D000332:Aerobiosis; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D009154:Mutation; D010100:Oxygen / Q000378:metabolism
chemical_list: D010100:Oxygen