Determination of phthalates in diet and bedding for experimental animals using gas chromatography-mass spectrometry.
We have developed a gas chromatography-mass spectrometry method to measure five phthalates (dibutyl phthalate, butylbenzyl phthalate, di-2-ethylhexyl phthalate, diisooctyl phthalate, and diisononyl phthalate) in diets and beddings for experimental animals. The recoveries from diets and beddings spiked with five phthalates were 98.8%-148% with coefficients of variation of 0.4%-7.8% for diets and 94.7%-146% with coefficients of variation of 1.0%-5.0% for beddings. We analyzed commercial animal diets and beddings, and found that the levels of phthalates varied from sample to sample; the concentrations of five phthalates were 141-1,410 ng/g for diets and 20.5-7,560 ng/g for beddings.
Kondo
Fumio
F
Department of Toxicology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan. fumio@ipc-tokai.or.jp
Okumura
Masanao
M
Oka
Hisao
H
Nakazawa
Hiroyuki
H
Izumi
Shun-Ichiro
S
Makino
Tsunehisa
T
D000388:Air / Q000032:analysis; D000393:Air Pollutants / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000830:Animals, Laboratory; D002138:Calibration; D004032:Diet; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry; D010795:Phthalic Acids / Q000032:analysis*; D012015:Reference Standards; D012377:Rodentia; D014867:Water / Q000032:analysis; D014873:Water Pollutants / Q000032:analysis
D000393:Air Pollutants; D010795:Phthalic Acids; D014873:Water Pollutants; D014867:Water