Authentication of the oriental medicinal plant Ligusticum tenuissimum (Nakai) Kitagawa (Korean Go-Bon) by multiplex PCR.
The oriental medicinal plant Ligusticum tenuissimum (Korean name, Go-Bon) is widely used in Korea and China. L. tenuissimum (Go-Bon) has been employed in the treatment of headache and common cold, and as a fever remedy. The internal transcribed spacer (ITS) region was sequenced from thirty-four Go-Bon samples collected from botanical gardens and markets in Korea and China to identify and authenticate L. tenuissimum. Based on the ITS sequences, the thirty-four Go-Bon samples were classified into three groups: L. tenuissimum (Korean Go-Bon), L. jeholense (Chinese Go-Bon), and unknown Chinese Ligusticum species. Three specific primers were designed to identify the three groups of Ligusticum species using multiplex PCR. The established multiplex-PCR was proved to be effective for the differentiation of L. tenuissimum in commercial plant materials.
Jigden
Baigalmaa
B
Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Giheung-gu Yongin-si, Gyeonggi-do, South Korea.
Wang
Hongtao
H
Kyum Kim
Myung
M
Kim
Yeon-Ju
YJ
Gyo In
Jun
J
Yang
Deok-Chun
DC
D021901:DNA, Intergenic / Q000235:genetics; D018744:DNA, Plant; D030004:Ligusticum / Q000145:classification* / Q000235:genetics*; D055670:Medicine, Korean Traditional; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction
D021901:DNA, Intergenic; D018744:DNA, Plant