title: Mitochondria positioning controls local calcium influx in T cells.
abstract: Formation of an immunological synapse (IS) between APC and T cells activates calcium entry through ORAI channels, which is indispensable for T cell activation. Successful proliferation and maturation of naive T cells is possible only if premature inactivation of ORAI channels is prevented. Although it is undisputed that calcium entry through ORAI channels is required for T cell function, it is not known if calcium influx is uniformly distributed over the plasma membrane or if preferential local calcium entry sites (for instance, at the IS) exist. In this study, we show that mitochondrial positioning determines the magnitude of local calcium entry anywhere in the plasma membrane by reducing local calcium-dependent channel inactivation: if mitochondria are close to any given local calcium entry site, calcium influx is large; if they are not close, calcium influx is small. Following formation of the IS, mitochondria are preferentially translocated to the IS in a calcium influx-dependent manner but independent of the exact calcium influx site. Mitochondrial enrichment at the IS favors local calcium entry at the IS without the necessity to enrich ORAI channels at the IS. We conclude that local calcium entry rather than global calcium entry is the preferential mechanism of calcium entry at stable ISs in Th cells.
authors: Department of Biophysics, University of the Saarland, Homburg, Germany.
authors: Christian
authors: C
authors: Schwindling
authors: Ariel
authors: A
authors: Quintana
authors: Elmar
authors: E
authors: Krause
authors: Markus
authors: M
authors: Hoth
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D054992:Immunological Synapses / Q000502:physiology*; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000276:immunology*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D000071740:ORAI1 Protein; D018408:Patch-Clamp Techniques; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D000071740:ORAI1 Protein; C520430:Orai1 protein, mouse; D002118:Calcium