Poly(L-lysine) nanostructured particles for gene delivery and hormone stimulation.
In this work, we designed replica particles based on poly (L-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function. PLL replica particles (PLL(RP)) were prepared by infiltrating polymer into mesoporous silica (MS) particles, crosslinking the adsorbed chains by using a homobifunctional crosslinker and finally removing the template particles. Moreover, we verified their cytotoxicity. Furthermore, based on this PLL(RP) gene delivery system, we simultaneously evaluated the melanin stimulation and gene expression in these cells by fluorescence microscopy. To further understand the bi-functionality, we labeled the SPT7pTL and PGA-alpha-MSH with YOYO-1 and Rhodamine, respectively, to follow its intracellular pathway by confocal microscopy. Our data suggests that the PLL(RP) is a promising vector for gene therapy and hormone stimulation.
Zhang
Xin
X
Institut National de la Santé et de la Recherche Médicale, INSERM, Unité 977, Faculté de Médecine, 11 Rue Humann, 67085 Strasbourg Cedex, France.
Oulad-Abdelghani
Mustapha
M
Zelkin
Alexander N
AN
Wang
Yajun
Y
Haîkel
Youssef
Y
Mainard
Didier
D
Voegel
Jean-Claude
JC
Caruso
Frank
F
Benkirane-Jessel
Nadia
N
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018014:Gene Transfer Techniques; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010957:Plasmids / Q000235:genetics; D011099:Polyglutamic Acid / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D000521:alpha-MSH / Q000378:metabolism / Q000494:pharmacology*
D008543:Melanins; D011107:Polylysine; D011099:Polyglutamic Acid; D000521:alpha-MSH