Systematic analysis of attenuated Coxsackievirus expressing a foreign gene as a viral vaccine vector.
Recombinant viruses expressing foreign antigens may provide a convenient vaccine vector capable of inducing preventative immunity. In this study, we explored the capacity of highly attenuated Coxsackievirus B3 (CVB3) to act as a recombinant vector to deliver foreign genes into experimental animals for the purpose of vaccination. The infectious cDNA of highly attenuated CVB3, YYFF, which has been reported previously (Vaccine 27:1974), was used to construct a recombinant YYFF cDNA (YYFF-HCV) by inserting a truncated form of hepatitis C virus (HCV) envelope protein E2 as an antigenic marker immediately upstream from the gene encoding the VP4 capsid protein. In YYFF-HCV-infected HeLa cells, HCV E2 expression was confirmed by immunoblotting and fluorescence microscopy. YYFF-HCV induced the production of antibodies and the cytotoxic T-lymphocyte (CTL) response to HCV E2 in the inoculated mice. Moreover, YYFF-HCV induced no inflammation in the virus-immunized mice. These results demonstrate that recombinant CVB3 expressing a foreign gene can act as a live vaccine vector capable of inducing humoral and cell-mediated immune responses directed against a foreign protein.
Kim
Dae-Sun
DS
Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Republic of Korea.
Cho
Young-Joo
YJ
Kim
Byoung-Guk
BG
Lee
Su-Hae
SH
Nam
Jae-Hwan
JH
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D019556:COS Cells; D002522:Chlorocebus aethiops; D029822:Enterovirus B, Human / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D006367:HeLa Cells; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D018937:Hepatitis C Antibodies; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines