[Two recombinant adenovirus vaccine candidates containing neuraminidase Gene of H5N1 influenza virus (A/Anhui/1/2005) elicited effective cell-mediated immunity in mice].
The aim of this study is to develop the recombinant adenovirus vaccine (rAdV) candidates containing neuraminidase (NA) gene of H5N1 influenza virus and test in BALB/c mice the effect of cell-mediated immunity. In this study, two kind of NA gene (WtNA gene, the wild type; Mod. NA gene, the codon-modified type) derived from H5N1 influenza virus (A/Anhui/1/2005) were cloned and inserted respectively into plasmid of adenovirus vector, then the rAdV vaccines candidates (rAdV-WtNA and rAdV-Mod. NA) were developed and purified, followed by immunization intramuscularly (10(9) TCID50 per dose, double injection at 0 and 4th week) in BALB/c mice, the effect of cell-mediated immunity were analysed at 5th week. Results indicated that: (i) NA protein expression was detected in two rAdV vaccines candidates by Western blotting; (ii) the rAdV-Mod. NA vaccine could elicit more robust NA specific cell-mediated immunity in mice than that of rAdV-WtNA vaccine (P = 0. 016) by IFN-gamma ELIspot assay. These findings suggested rAdV-Mod. NA vaccine was a potential vaccine candidate against H5N1 influenza and worthy of further investigation.
Ma
Jing
J
The College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China.
Zhang
Xiao-Guang
XG
Chen
Hong
H
Li
Kui-Biao
KB
Zhang
Xiao-Mei
XM
Zhang
Ke
K
Yang
Liang
L
Xu
Hong
H
Shu
Yue-Long
YL
Tan
Wen-Jie
WJ
Zeng
Yi
Y
D000818:Animals; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000821:virology; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D007252:Influenza Vaccines; D014764:Viral Proteins; D009439:Neuraminidase