Relationship between microtensile bond strength and submicron hiatus at the composite-dentin interface using CLSM visualization technique.
OBJECTIVE
"Submicron hiatus" represents a potential space between the base of the collagenous network and the mineralized dentin when it is acid etched for bonding. This study evaluated the relationship between microtensile bond strength (microTBS) and occurrence of submicron hiatus formations at the resin-dentin interface using the same specimens.


METHODS
Resin-dentin bonded micro-specimens (sticks with a size of 300 microm x 300 microm x 8mm) were prepared using one of two material combinations (group I: Syntac Classic/Tetric Ceram Cavifil: n=51 group II: Prime & Bond NT/Tetric Ceram Cavifil: n=56). After labeling the primer component with a tiny amount of rhodamine-B-isothiocyanate, submicron hiatus formations were imaged nondestructively using a confocal laser scanning microscope (CLSM). Subsequently specimens were subjected to a microTBS test.


RESULTS
For the influence of submicron hiatus formations on microTBS with the Syntac Classic group, the nonparametric Spearman's correlation was -0.329 at p=0.02. For the Prime & Bond NT group, the nonparametric Spearman's correlation was -0.356 at p=0.007. Analyzing the effect of submicron hiatus on without discriminating by group resulted in a Spearman's correlation coefficient of -0.341 at p=0.001; microTBS and quality of hybrid layer showed a correlation coefficient of 0.849 at p=0.001, and microTBS and quality of tag formation showed a correlation coefficient of 0.474 at p=0.001.


CONCLUSIONS
The degree of submicron hiatus formations had an influence on microtensile bond strength for both the Syntac Classic and the Prime & Bond NT group.
Ding
Paul G F
PG
Department of Conservative Dentistry, Clinic for Oral, Dental and Maxillofacial Diseases, University Hospital Heidelberg, Germany. paul.ding@med.uni-heidelberg.de
Matzer
Axel R A H
AR
Wolff
Diana
D
Mente
Johannes
J
Pioch
Thomas
T
Staehle
Hans Jörg
HJ
Dannewitz
Bettina
B
D000134:Acid Etching, Dental; D003094:Collagen; D003188:Composite Resins; D001840:Dental Bonding; D003793:Dental Restoration, Permanent / Q000379:methods; D003799:Dental Stress Analysis; D003804:Dentin; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D011109:Polymethacrylic Acids; D019279:Resin Cements; D018709:Statistics, Nonparametric; D013718:Tensile Strength
D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C078484:Syntac dentine adhesive; D003094:Collagen