title: High-throughput generation of selected reaction-monitoring assays for proteins and proteomes.
abstract: Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
authors: Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Zurich, Switzerland.
authors: Paola
authors: P
authors: Picotti
authors: Oliver
authors: O
authors: Rinner
authors: Robert
authors: R
authors: Stallmach
authors: Franziska
authors: F
authors: Dautel
authors: Terry
authors: T
authors: Farrah
authors: Bruno
authors: B
authors: Domon
authors: Holger
authors: H
authors: Wenschuh
authors: Ruedi
authors: R
authors: Aebersold
mesh_terms: D001681:Biological Assay / Q000379:methods*; D030562:Databases, Protein; D057166:High-Throughput Screening Assays / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D019151:Peptide Library; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D019151:Peptide Library; D011506:Proteins; D020543:Proteome; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases