title: Application of Fourier-transform infrared (FT-IR) spectroscopy for simple and easy determination of chylomicron-triglyceride and very low density lipoprotein-triglyceride.
abstract: BACKGROUND
  Fourier-transform infrared (FT-IR) spectroscopy is a simple and reagent-free physicochemical analysis method, and is a potential alternative to more time-consuming and labor-intensive procedures. In this study, we aimed to use FT-IR spectroscopy to determine serum concentrations of chylomicron-triglyceride (TG) and very low density lipoprotein (VLDL)-TG.
  
  
  METHODS
  We analyzed a chylomicron fraction and VLDL fraction, which had been obtained by ultracentrifugation, to search for wavelengths to designate to each fraction. Then, partial least square (PLS) calibrations were developed using a training set of samples, for which TG concentrations had been determined by conventional procedures. Validation was conducted with another set of samples using the PLS model to predict serum TG concentrations on the basis of the samples' IR spectra. We analyzed a total of 150 samples.
  
  
  RESULTS
  Serum concentrations of chylomicron-TG and VLDL-TG estimated by FT-IR spectroscopy agreed well with those obtained by the reference method (r=0.97 for both lipoprotein fractions). FT-IR spectrometric analysis required 15mul of serum and was completed within 1min.
  
  
  CONCLUSIONS
  Serum chylomicron-TG and VLDL-TG concentrations can be determined with FT-IR spectroscopy. This rapid and simple test may have a great impact on the management of patients with dyslipidemia.
authors: Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Japan.
authors: Kenichi
authors: K
authors: Sato
authors: Masanori
authors: M
authors: Seimiya
authors: Yoshio
authors: Y
authors: Kodera
authors: Akihide
authors: A
authors: Kitamura
authors: Fumio
authors: F
authors: Nomura
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002914:Chylomicrons / Q000097:blood*; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood*; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014280:Triglycerides / Q000097:blood*
chemical_list: D002914:Chylomicrons; D008079:Lipoproteins, VLDL; D014280:Triglycerides