title: Characterisation of G8 human rotaviruses in Australian children with gastroenteritis.
abstract: This study describes the characterisation of G8 rotavirus strains isolated from humans with acute gastroenteritis. Six G8 strains were detected in Australia between 2002 and 2008. Four were G8P[14] strains, one was G8P[8]+[14] and one was G8 P non-typeable. By polyacrylamide gel electrophoresis and enzyme immunoassay analysis, four G8 strains with visible RNA exhibited a long electropherotype and five G8 strains displayed subgroup I specificity. Sequence analysis of the VP7 gene indicated that the G8 strains exhibited the highest nucleotide and amino acid identity with a G8P[11] bovine rotavirus strain detected in Japan. VP4 sequence data of one G8P[14] strain revealed that the closest identity was to another human-bovine-like strain detected in Australia, MG6, a G6P[14] strain. The identification of G8 strains causing disease further extends the number of G8P[14] strains detected in Australian children, and indicates that there is a rare but ongoing presence of uncommon human strains within the community in Australia.
authors: Melbourne School of Land and Environment, The University of Melbourne, Parkville, Victoria, Australia.
authors: Dayna L
authors: DL
authors: Swiatek
authors: Enzo A
authors: EA
authors: Palombo
authors: Alvin
authors: A
authors: Lee
authors: Michael J
authors: MJ
authors: Coventry
authors: Margaret L
authors: ML
authors: Britz
authors: Carl D
authors: CD
authors: Kirkwood
mesh_terms: D000595:Amino Acid Sequence; D001315:Australia / Q000453:epidemiology; D002648:Child; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins