title: Degradable polymers for gene delivery.
abstract: Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure led to significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than Lipofectamine 2000 or polyethylenimine for gene delivery to cancerous fibroblasts or human primary fibroblasts. These materials may be useful for cancer therapeutics and regenerative medicine.
authors: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Joel
authors: J
authors: Sunshine
authors: Nupura
authors: N
authors: Bhise
authors: Jordan J
authors: JJ
authors: Green
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004337:Drug Carriers; D005347:Fibroblasts / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000295:instrumentation; D006801:Humans; D008055:Lipids / Q000737:chemistry; D009369:Neoplasms / Q000628:therapy*; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D044968:Regenerative Medicine / Q000379:methods
chemical_list: D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D011108:Polymers; D011094:Polyethyleneimine