title: Reliability of assessing dye penetration along root canal fillings using methylene blue.
abstract: Methylene blue (MB) remains the most frequently used tracer for dye penetration tests of endodontic fillings, despite its chemical reactions with different materials. This study checked whether dye penetration displayed by MB is comparable to Rhodamine B (RB). One hundred and seventy-two root canals were filled with gutta-percha and six sealers. Samples were covered with nail varnish except for the apical area, and immersed in MB or RB solutions under negative pressure. After 24 h, roots were bisected, photographed and the maximal dye penetration recorded. Dye penetrations displayed by MB and RB were compared (Newman-Keuls test). MB and RB displayed comparable results for groups filled with AH Plus, EndoREZ and Polifil (P > 0.05). For Endofill, Sealer 26 and Sealapex, a significant lower dye penetration was observed when MB was used (P < 0.05). Laboratory tests using MB for measuring dye penetration through filled root canals can result in misleading conclusions.
authors: Postgraduation Section, University Center of Maranhão UNICEUMA, São Luis, MA, Brazil.
authors: Erick Miranda
authors: EM
authors: Souza
authors: Fernanda Geraldes
authors: FG
authors: Pappen
authors: Hagay
authors: H
authors: Shemesh
authors: Cristiane
authors: C
authors: Bonanato-Estrela
authors: Idomeo
authors: I
authors: Bonetti-Filho
mesh_terms: D001729:Bismuth / Q000737:chemistry; D002126:Calcium Hydroxide / Q000737:chemistry; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003763:Dental Leakage / Q000145:classification*; D004853:Epoxy Resins / Q000737:chemistry; D005456:Fluorescent Dyes; D006171:Gutta-Percha / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008751:Methylene Blue; D015203:Reproducibility of Results; D012235:Rhodamines; D012387:Root Canal Filling Materials / Q000737:chemistry*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012459:Salicylates / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D003188:Composite Resins; C481259:EndoRez; D004853:Epoxy Resins; D005456:Fluorescent Dyes; D012235:Rhodamines; D012387:Root Canal Filling Materials; D012459:Salicylates; C080620:Sealer 26; C534916:epoxy resin-based root canal sealer; C532269:polifil; C074417:Endo-Fill; C055601:Sealapex; D006171:Gutta-Percha; C029773:rhodamine B; D002126:Calcium Hydroxide; D008751:Methylene Blue; D001729:Bismuth