title: Epidemiological link between gastric disease and polymorphisms in VacA and CagA.
abstract: Gastritis, peptic ulcer disease, and gastric cancer are a few of the diverse disease manifestations that have been shown to be associated with infection by Helicobacter pylori. Why some individuals develop more severe forms of disease remains largely unknown. In this study, 225 South Korean strains were genotyped for vacA and then analyzed to determine if particular genotypes varied across disease state, sex, or cagA allele. Of these strains, 206 strains carried an s1/i1/m1 allele, 11 strains carried an s1/i1/m2 allele, and 8 strains carried an s1/i2/m2 allele. By using Fisher's exact test, a statistical association between variations in the cagA and vacA alleles was identified (P = 0.0007), and by using log linear modeling, this variation was shown to affect the severity of disease outcome (P = 0.027). Additionally, we present evidence that variation within the middle region of VacA contributes significantly to the distribution of vacA alleles across gender (P = 0.008) as well as the association with disease outcome (P = 0.011). In this South Korean population, the majority of H. pylori strains carry the vacA s1/i1/m1 allele and the CagA EPIYA-ABD allele. These facts may contribute to the high incidence of gastric maladies, including gastric cancer.
authors: Department of Oral Biology, Oral Science Research Center, BK21 Project, Yonsei University College of Dentistry, Seoul, South Korea.
authors: Sungil
authors: S
authors: Jang
authors: Kathleen R
authors: KR
authors: Jones
authors: Cara H
authors: CH
authors: Olsen
authors: Young Min
authors: YM
authors: Joo
authors: Yun-Jung
authors: YJ
authors: Yoo
authors: In-Sik
authors: IS
authors: Chung
authors: Jeong-Heon
authors: JH
authors: Cha
authors: D Scott
authors: DS
authors: Merrell
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005756:Gastritis / Q000453:epidemiology* / Q000382:microbiology; D005787:Gene Frequency; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010437:Peptic Ulcer / Q000453:epidemiology* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori