title: Expression of the atf1+ gene is upregulated in fission yeast under nitrosative and nutritional stresses.
abstract: This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 micromol/L) and nitrogen depletion significantly enhanced synthesis of beta-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 micromol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of beta-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.
authors: Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, 192-1 Hyoja-2-dong, Chuncheon 200-701, Korea.
authors: S-H
authors: SH
authors: Song
authors: B-M
authors: BM
authors: Kim
authors: C-J
authors: CJ
authors: Lim
authors: Y S
authors: YS
authors: Song
authors: E-H
authors: EH
authors: Park
mesh_terms: D051697:Activating Transcription Factor 1 / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009584:Nitrogen / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D051697:Activating Transcription Factor 1; D050976:Basic-Leucine Zipper Transcription Factors; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; D010750:Phosphoproteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; C106740:atf1 protein, S pombe; D009584:Nitrogen