title: Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay.
abstract: OBJECTIVE
  The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1.
  
  
  METHODS
  Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level.
  
  
  RESULTS
  Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61% of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1% caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1% extract.
  
  
  CONCLUSIONS
  The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.
authors: Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil.
authors: Gabriela Tavares Bekner
authors: GT
authors: Correa
authors: Gabriel Alves Costa
authors: GA
authors: Veranio
authors: Licínio Esmaeraldo
authors: LE
authors: Silva
authors: Raphael
authors: R
authors: Hirata Junior
authors: Jeffry M
authors: JM
authors: Coil
authors: Miriam F Zaccaro
authors: MF
authors: Scelza
mesh_terms: D001466:Barium Sulfate / Q000633:toxicity; D001729:Bismuth / Q000633:toxicity; D001881:Borates / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity*; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005054:Eugenol / Q000633:toxicity; D006801:Humans; D008422:Materials Testing; D009000:Monocytes / Q000187:drug effects; D012117:Resins, Synthetic / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D014343:Trypan Blue; D015034:Zinc Oxide / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D001881:Borates; D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C534916:epoxy resin-based root canal sealer; D001466:Barium Sulfate; D005054:Eugenol; C038425:Fillcanal; D014343:Trypan Blue; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D001729:Bismuth