title: Interaction of Cowpea mosaic virus (CPMV) nanoparticles with antigen presenting cells in vitro and in vivo.
abstract: BACKGROUND
  Plant viruses such as Cowpea mosaic virus (CPMV) are increasingly being developed for applications in nanobiotechnology including vaccine development because of their potential for producing large quantities of antigenic material in plant hosts. In order to improve efficacy of viral nanoparticles in these types of roles, an investigation of the individual cell types that interact with the particles is critical. In particular, it is important to understand the interactions of a potential vaccine with antigen presenting cells (APCs) of the immune system. CPMV was previously shown to interact with vimentin displayed on cell surfaces to mediate cell entry, but the expression of surface vimentin on APCs has not been characterized.
  
  
  METHODS
  The binding and internalization of CPMV by several populations of APCs was investigated both in vitro and in vivo by flow cytometry and fluorescence confocal microscopy. The association of the particles with mouse gastrointestinal epithelium and Peyer's patches was also examined by confocal microscopy. The expression of surface vimentin on APCs was also measured.
  
  
  CONCLUSIONS
  We found that CPMV is bound and internalized by subsets of several populations of APCs both in vitro and in vivo following intravenous, intraperitoneal, and oral administration, and also by cells isolated from the Peyer's patch following gastrointestinal delivery. Surface vimentin was also expressed on APC populations that could internalize CPMV. These experiments demonstrate that APCs capture CPMV particles in vivo, and that further tuning the interaction with surface vimentin may facilitate increased uptake by APCs and priming of antibody responses. These studies also indicate that CPMV particles likely access the systemic circulation following oral delivery via the Peyer's patch.
authors: Department of Cell Biology, The Scripps Research Institute, La Jolla, California, United States of America.
authors: Maria J
authors: MJ
authors: Gonzalez
authors: Emily M
authors: EM
authors: Plummer
authors: Chris S
authors: CS
authors: Rae
authors: Marianne
authors: M
authors: Manchester
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000378:metabolism* / Q000821:virology*; D001709:Biotechnology / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D017798:Comovirus / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods; D053758:Nanoparticles; D036103:Nanotechnology / Q000379:methods*; D011485:Protein Binding; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism
chemical_list: D014746:Vimentin