Bruch's membrane and choroidal macrophages in early and advanced age-related macular degeneration.
OBJECTIVE
To determine the sub-macular Bruch's membrane (BrM) macrophage count and the choroidal and BrM macrophage immunophenotype in normal eyes and in eyes with early and advanced age-related macular degeneration (AMD).


METHODS
BrM macrophages were counted in 125 human eyes (normal, normal aged, early AMD and geographical atrophy), and CD68 and inducible nitric oxide synthase (iNOS) immunohistochemistry was performed on 16 human eyes (normal, normal aged, early AMD, geographical atrophy and disciform scarring). All eyes were examined clinically ante mortem. Results were correlated with histopathological features, including basal laminar deposit and membranous debris, and with clinical fundus appearance.


RESULTS
CD68(+) macrophages were found in the choroid of normal human eyes, and did not express iNOS. Expression of iNOS by choroidal macrophages (as well as endothelial cells and pericytes) was associated with: (1) recruitment of macrophages to BrM in early AMD eyes with soft drusen or thick continuous basal laminar deposit, corresponding to clinically detectable soft drusen or pigment changes; and (2) active disciform scarring. iNOS expression was absent in BrM macrophages, suggesting immunomodulatory differences between the choroid and BrM. The highest BrM macrophage counts were found in eyes with subclinical choroidal neovascularisation.


CONCLUSIONS
The presence of extracellular deposits (soft drusen and thick continuous basal laminar deposit) is associated with macrophage recruitment to BrM and alteration in the immunophenotype of resident choroidal macrophages.
Cherepanoff
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Anatomical Pathology, SEALS, Prince of Wales Hospital, Level 4, Campus Centre, Randwick, NSW, Australia. svetlana.cherepanoff@sesiahs.health.nsw.gov.au
McMenamin
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Gillies
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MC
Kettle
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Sarks
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SH
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D016570:Bruch Membrane / Q000276:immunology / Q000473:pathology*; D002452:Cell Count; D002829:Choroid / Q000276:immunology / Q000473:pathology*; D020256:Choroidal Neovascularization / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D018450:Disease Progression; D057092:Geographic Atrophy / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D016130:Immunophenotyping; D008264:Macrophages / Q000276:immunology / Q000473:pathology*; D008268:Macular Degeneration / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D014792:Visual Acuity; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D052247:Nitric Oxide Synthase Type II; C067980:CD68 antigen, human; D000097382:CD68 Molecule