title: Yeast acetic acid-induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1.
abstract: To investigate the role of cytochrome c (cyt c) release in yeast acetic acid-induced programmed cell death (AA-PCD), wild type (wt) and cells lacking metacaspase (Deltayca1), cytochrome c (Deltacyc1,7) and both (Deltacyc1,7Deltayca1) were compared for AA-PCD occurrence, hydrogen peroxide (H(2)O(2)) production and caspase activity. AA-PCD occurs in Deltacyc1,7 and Deltacyc1,7Deltayca1 cells slower than in wt, but similar to that in Deltayca1 cells, in which no cytochrome c release occurs. Both H(2)O(2) production and caspase activation occur in these cells with early and extra-activation in Deltacyc1,7 cells. We conclude that alternative death pathways can be activated in yeast AA-PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it.
authors: CNR, Istituto di Biomembrane e Bioenergetica, Bari, Italy.
authors: Nicoletta
authors: N
authors: Guaragnella
authors: Antonella
authors: A
authors: Bobba
authors: Salvatore
authors: S
authors: Passarella
authors: Ersilia
authors: E
authors: Marra
authors: Sergio
authors: S
authors: Giannattasio
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid