Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP.
To gain insights into the mechanisms of drug resistance in esophageal squamous cell carcinoma (ESCC), we employed proteomic techniques to study the global protein change of the multi-drug resistant ESCC cell line EC109/CDDP, which was established in our previous work, in comparison with its parental drug sensitive cell line EC109. By two-dimensional electrophoresis and mass spectrometry, we successfully identified 44 proteins with altered expression levels. These proteins are involved in endoplasmic reticulum stress response, metabolic process, DNA replication and repair, nucleotide binding, calcium binding, and cytoskeletal proteins. Among them, the differential expression levels of thioredoxin domain-containing protein 4 precursor and cystathionine gamma-lyase were further validated by Western blot and RT-PCR. Our present results lay foundation for future in-depth work on molecular mechanism of ESCC drug resistance, and aid in the identification and use of novel markers in clinical practice.
Wen
Jing
J
Department of Thoracic Oncology, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, P.R. China.
Zheng
Bin
B
Hu
Yi
Y
Zhang
Xu
X
Yang
Hong
H
Li
Yan
Y
Zhang
Chun-Yu
CY
Luo
Kong-Jia
KJ
Zang
Xing
X
Li
Yong-Feng
YF
Guan
Xin-Yuan
XY
Fu
Jian-Hua
JH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D003542:Cystathionine gamma-Lyase / Q000096:biosynthesis; D003599:Cytoskeleton / Q000378:metabolism; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods*
D000970:Antineoplastic Agents; D003542:Cystathionine gamma-Lyase; D002945:Cisplatin