title: Pouch colon associated with anorectal malformations fails to show spontaneous contractions but responds to acetylcholine and histamine in vitro.
abstract: OBJECTIVE
  Congenital pouch colon (CPC) associated with anorectal malformation (ARM) is most commonly reported from Northern India. So far, no physiologic study comparing the detailed contractile status of CPC with non-CPC conditions are available. The present article deals with the contractile study and histopathologic observations in CPC, which may be useful for better surgical management.
  
  
  METHODS
  Freshly excised 12 neonatal CPC and similar number of non-CPC (control) specimens were transferred to ice-cold (4 degrees C-6 degrees C) Krebs-Ringer solution bubbled with 100% oxygen. Longitudinally prepared 2 to 4 colonic strips were obtained from central part of each specimen and subjected to the contraction recording after exposure to cumulative concentrations of acetylcholine (ACh) and histamine. Acetylcholine-induced contractions were evaluated after application of atropine (muscarinic blocker), and histaminergic contractions were recorded after pheniramine (H(1) blocker), lignocaine (neuronal blocker), and atropine. Histopathologic observations were made by using H&E and Masson trichrome stains.
  
  
  RESULTS
  Control specimens showed spontaneous contractions, but CPC strips did not. Both control and CPC responded to ACh and histamine. The response to histamine was greater (P < .05) in CPC as compared to control, whereas the response to ACh was more (P < .05) in control. In CPC, response of histamine (100 micromol/L) was blocked by pheniramine (0.32 mmol/L) and lignocaine (4 mmol/L) by 97% and 80%, respectively, and enhanced by 57% after preapplication of atropine (10 micromol/L). Acetylcholine (100 micromol/L)-induced contractions were attenuated (86%) in presence of atropine. Histopathologic examination showed fewer mature ganglion cells with various changes in muscle layers including fibrosis, disruption, hypertrophy, atrophy, and constriction bands.
  
  
  CONCLUSIONS
  Congenital pouch colon associated with ARM lacks normal spontaneous contractions but retains ACh and histamine-induced contractility. In view of the functional and histologic abnormalities, we propose that CPC associated with ARM is an abnormally functional and developed tissue. Therefore, resection of the pouch should be considered for better functional outcome of the remaining bowel.
authors: Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, UP, India.
authors: Preeti
authors: P
authors: Tyagi
authors: Maloy B
authors: MB
authors: Mandal
authors: Sanchayan
authors: S
authors: Mandal
authors: Shashikant C U
authors: SC
authors: Patne
authors: Ajay N
authors: AN
authors: Gangopadhyay
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D001003:Anal Canal / Q000002:abnormalities / Q000187:drug effects; D001285:Atropine / Q000494:pharmacology; D003106:Colon / Q000002:abnormalities* / Q000187:drug effects* / Q000473:pathology; D004241:Diverticulum, Colon / Q000151:congenital* / Q000473:pathology / Q000601:surgery; D005769:Gastrointestinal Motility / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D056885:Interstitial Cells of Cajal / Q000502:physiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000502:physiology; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology
chemical_list: D011969:Receptors, Histamine H1; D011976:Receptors, Muscarinic; D001285:Atropine; D006632:Histamine; D000109:Acetylcholine