[Effects of heroin and purine nucleotide compensation on neutral alpha-glucosidase in rat epididymides].
OBJECTIVE
To study the changes of neutral alpha-glucoside activity in the epididymis of heroin-dependent and heroin-withdrawal rats, and to investigate the effects of intervention with purine nucleotide (AMP and GMP).


METHODS
Eighty Wistar rats were randomly divided into 8 groups of equal number, control, nucleotide, heroin, heroin + nucleotide, 3 d withdrawal, 9 d withdrawal, 3 d nucleotide (nucleotide administrated for 3 days after heroin withdrawal) and 9 d nucleotide (nucleotide administrated for 9 days after heroin withdrawal). Neutral alpha-glucosidase activity in the epididymis was detected in each group of rats.


RESULTS
Compared with the control group, neutral alpha-glucoside activity was markedly decreased in the heroin group (P < 0.05), and also in the 3 d and 9 d withdrawal groups, although with no significant differences (P > 0.05).


CONCLUSIONS
Heroin reduces neutral alpha-glucoside activity in the epididymis of rats, and this effect may continue for some time after drug withdrawal, while purine nucleotide can keep neutral alpha-glucosidase activity in a relatively stable state.
Cui
Jia-yue
JY
Department of Histology and Embryology, Norman Bethune College of Medicine, Jilin University, Changchun, Jilin 130021, China. cuijiayue@jlu.edu.cn
Zhang
Jia-ying
JY
Zuo
Wen-jing
WJ
Wang
Zhong-shan
ZS
Hong
Min
M
D000818:Animals; D004822:Epididymis / Q000737:chemistry*; D003932:Heroin / Q000009:adverse effects*; D006556:Heroin Dependence / Q000378:metabolism*; D008297:Male; D011685:Purine Nucleotides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000520:alpha-Glucosidases / Q000378:metabolism*
D011685:Purine Nucleotides; D003932:Heroin; D000520:alpha-Glucosidases