Dual compartment neurofluidic system for electrophysiological measurements in physically isolated neuronal cell cultures.
This work investigates an approach to record electrophysiological measurements of neuronal cell cultures in a dual compartment neurofluidic system. The two compartments are separated by 10-microm-wide and 3-microm-high microchannels and this provides a physical isolation of neurons allowing only neurites to grow between the compartments. We present long-term cell viability in closed compartment devices, neurite growth across the microchannels and a recording setup for the long-term recording of the network activity over 21 Days-in-Vitro (DIV). Structural and fluidic isolation between the compartments are demonstrated using transfection experiments and neurotoxin exposure, respectively.
Kanagasabapathi
Thirukumaran T
TT
Department of Minimally Invasive Healthcare, Philips Research Laboratories Eindhoven, High Tech Campus 34.2(063), 5656 AE Eindhoven, The Netherlands. thiru.kanagasabapathi@Philips.com
Wang
Ke
K
Mellace
Marco
M
Ramakers
Ger J A
GJ
Decre
Michel M J
MM
D002478:Cells, Cultured; D044085:Microfluidics; D009474:Neurons / Q000502:physiology*