[Identification of a novel HLA allele HLA-B*9526].
OBJECTIVE
A novel human leukocyte antigen-B (HLA-B) allele, B*9526, was identified and analyzed by sequencing-based method in a Chinese donor.


METHODS
HLA typing was performed by PCR-sequence-specific oligonucleotide (SSO). Molecular cloning and DNA sequencing were used to identify the sequence of the potential novel allele and the difference between this new allele and other known alleles were analyzed.


RESULTS
HLA genotyping of one sample gave different results. The sequencing result showed HLA-B alleles of the proband as B*5403 and a novel allele. The nucleotide sequence of the novel allele was different from all known B alleles and harbored one nucleotide change from the closest matching allele B*1507 at nucleotide 425 (A to G) in exon 3, resulting in an amino acid change from Y (TAC) to C (TGC) at codon 142.


CONCLUSIONS
A novel HLA allele was identified and officially designated as HLA-B*9526 by WHO Nomenclature Committee for Factors of the HLA System.
Chen
Yang
Y
Liaoning Blood Center, Shenyang, Liaoning, 110044 PR China.
Liu
Xianzhi
X
Li
Xiaofeng
X
Zhang
Xu
X
Zhang
Kunlian
K
Li
Jianping
J
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens; C540899:HLA-B*15:126 antigen; D059910:HLA-B15 Antigen