Imaging and quantitative assessment of long bone vascularization in the adult rat using microcomputed tomography.
The objective of this study was to develop and validate a technique for both 3D imaging and quantification of the vascular network of bone tissue in the rat. Five month-old male Wistar rats were divided into tail-suspension (21 days) and control groups. Sixty percent barium sulfate solution was infused into the vena cava. The tibiae were evaluated in 2D and 3D before and after decalcification, using conventional microcomputerized tomography (muCT) at 10 and 5 mum resolution and synchrotron radiation (SR) muCT. The perfusion technique and tomography exhibited excellent bone vasculature imaging. Significant positive correlations were observed between 2D histomorphometric and 3D muCT vascular parameters (P < 0.05). 3DmuCT discriminated significant changes of vessel structures in unloading condition: vessel number decreased by 25%, (P < 0.005), vessel separation increased by 27%, P < 0.01. SRmuCT could image sinusoid clusters in bone. muCT is an accurate and reproducible technique for 3D quantitative evaluation of long bone vascularisation in the rat.
Fei
Jia
J
Université de Lyon, France.
Jia
Fei
F
Peyrin
Françoise
F
Françoise
Peyrin
P
Malaval
Luc
L
Vico
Laurence
L
Laurence
Vico
V
Lafage-Proust
Marie-Hélène
MH
Marie-Hélène
Lafage-Proust
LP
D000367:Age Factors; D000375:Aging; D000792:Angiography / Q000295:instrumentation* / Q000379:methods; D000818:Animals; D001466:Barium Sulfate / Q000008:administration & dosage; D001853:Bone Marrow / Q000098:blood supply*; D002196:Capillaries / Q000000981:diagnostic imaging; D003287:Contrast Media / Q000008:administration & dosage; D019417:Hindlimb Suspension; D021621:Imaging, Three-Dimensional; D007262:Infusions, Intravenous; D008297:Male; D011857:Radiographic Image Interpretation, Computer-Assisted; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D017356:Synchrotrons; D013977:Tibia / Q000098:blood supply*; D014684:Venae Cavae; D055114:X-Ray Microtomography / Q000295:instrumentation*
D003287:Contrast Media; D001466:Barium Sulfate