title: The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy.
abstract: Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy.
authors: Department of Histology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania. rodalighezan@yahoo.com
authors: Rodica
authors: R
authors: Lighezan
authors: Flavia
authors: F
authors: Baderca
authors: Aurora
authors: A
authors: Alexa
authors: M
authors: M
authors: Iacovliev
authors: Diana
authors: D
authors: Bonţe
authors: Elena Doina
authors: ED
authors: Murărescu
authors: Adriana
authors: A
authors: Nebunu
mesh_terms: D005557:Formaldehyde / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008853:Microscopy; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D009993:Osmium Tetroxide / Q000737:chemistry; D016612:Paraffin Embedding / Q000379:methods*; D016707:Tissue Fixation; D014571:Urologic Neoplasms / Q000648:ultrastructure*
chemical_list: D005557:Formaldehyde; D009993:Osmium Tetroxide; D005976:Glutaral