Lack of association between ACE ID polymorphism and colorectal cancer in Romanian patients.
BACKGROUND
The insertion/deletion polymorphism of the angiotensin I-converting enzyme (ACE) gene has recently been linked to the pathogenesis of human cancers. The goal of this study was to analyze the possible association between ACE gene I/D polymorphism and colorectal cancer in Romanian patients.


METHODS
Blood samples were obtained, after informed consent, from individuals with colorectal cancer (n=108, M:W = 64:44), and healthy persons (n=150, M:W = 84:66). Genomic DNA was extracted from peripheral blood leucocytes using commercial kits and the insertion (I) / deletion (D) polymorphism was assessed by PCR. Statistical analysis was done using the chi2 test. We determined the odds ratio using the genotype II as risk factor. A p value < 0.05 was considered statistically significant.


RESULTS
The distribution of ACE II: ID: DD genotypes was 23.1%: 46.3%: 30.6% in patients and respectively 20%: 48.7%: 31.3% in controls. The distribution of genotype (chi2 0.37, p = 0.54) and alleles (chi2 0.19, p = 0.65) did not differ significantly between cancer patients and control.


CONCLUSIONS
Study results do not demonstrate an association between ACE ID polymorphism and colorectal cancer in our patients.
Toma
M
M
Human Genetics Department, Institute of Genetics, University of Bucharest, Romania. iahim_t@yahoo.com
Cimponeriu
D
D
Apostol
P
P
Stavarachi
M
M
Cojocaru
M
M
Belusică
L
L
Crăciun
A M
AM
Radu
I
I
Gavrilă
L
L
D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D012383:Romania
D014408:Biomarkers, Tumor; D007703:Peptidyl-Dipeptidase A