title: Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed gamma-glutamylmethylamide synthetase and dried yeast cells.
abstract: Gamma-glutamylmethylamide synthetase and dried baker's yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mM theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD(+).
authors: Department of Chemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan. s_yamamoto@ks.kyorin-u.ac.jp
authors: Sachiko
authors: S
authors: Yamamoto
authors: Yosuke
authors: Y
authors: Morihara
authors: Mamoru
authors: M
authors: Wakayama
authors: Takashi
authors: T
authors: Tachiki
mesh_terms: D019731:Carbon-Nitrogen Ligases / Q000737:chemistry* / Q000378:metabolism*; D003956:Dialysis; D004735:Energy Transfer; D005285:Fermentation; D005971:Glutamates / Q000096:biosynthesis*; D008567:Membranes, Artificial; D012441:Saccharomyces cerevisiae / Q000166:cytology*
chemical_list: D005971:Glutamates; D008567:Membranes, Artificial; C026166:theanine; D019731:Carbon-Nitrogen Ligases; C020661:gamma-glutamylmethylamide synthetase