Labels and probes for live cell imaging: overview and selection guide.
Fluorescence imaging is an important tool for molecular biology research. There is a wide array of fluorescent labels and activatable probes available for investigation of biochemical processes at a molecular level in living cells. Given the large number of potential imaging agents and numerous variables that can impact the utility of these fluorescent materials for imaging, selection of the appropriate probes can be a difficult task. In this report an overview of fluorescent imaging agents and details on their optical and physical properties that can impact their function are presented.
Hilderbrand
Scott A
SA
Center for Molecular Imaging Research, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA, USA.
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000032:analysis / Q000378:metabolism; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D008670:Metals; D017382:Reactive Oxygen Species