Stimulation of glucose oxidase with white linearly polarized light.
Glucose oxidase (GOD) was illuminated with white linearly polarized light (WLPL). The enzyme was illuminated at room temperature in separate vessels then admixed to a reactor filled with D-glucose. The illumination of the enzyme for 60 min at 25-30 degrees C and pH 6.5-7.0 provided its superior stimulation as proven in the oxidation of beta-D-glucose. Lyophilization of the illuminated enzyme reduced its activity by, approximately, 30%.
Konieczna-Molenda
Anna
A
Dept. of Chemistry, Agricultural University, Krakow 30149, Poland. akonieczna-molenda@ar.krakow.pl
Fiedorowicz
Maciej
M
Tomasik
Przemysław J
PJ
D005612:Freeze Drying; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008027:Light; D013696:Temperature
D005949:Glucose Oxidase; D005947:Glucose