[The study of the creatine kinase radiation stability in 25Mg-enzyme enrichment experiment].
The radiation inactivation technique was applied to conduct the study on catalytic activity changes of creatine kinase species isolated from the Mid-Asian Death Adder venom. Both intact and the 25Mg-enriched enzyme samples were separately tested. Some differences in radiation stability levels were found to be indicating to a shift in conformational condition (a so called "conformational distribution") of the native enzyme that had happen in the presence of the magnetic nuclei excess.
Orlova
M A
MA
Kuznetsov
D A
DA
Latysheva
A V
AV
Orlov
A P
AP
D000818:Animals; D002384:Catalysis / Q000528:radiation effects; D003402:Creatine Kinase / Q000737:chemistry* / Q000528:radiation effects*; D008274:Magnesium / Q000737:chemistry*; D011487:Protein Conformation / Q000528:radiation effects; D011868:Radioisotopes / Q000737:chemistry; D014757:Viper Venoms / Q000201:enzymology*
D011868:Radioisotopes; D014757:Viper Venoms; D003402:Creatine Kinase; D008274:Magnesium