title: Various genotypes of Mycobacterium leprae from Mexico reveal distinct geographic distribution.
abstract: OBJECTIVE
  To classify Mycobacterium leprae isolates from multiple areas in Mexico based on variable number of tandem repeats of 6 base within the rpoT gene and three single nucleotide polymorphism (SNP), and to analyse their geographic distribution in the context of the origin of leprosy in Mexico.
  
  
  RESULTS
  Analysis for rpoT genotyping of 64 samples collected in the west and southwestern areas revealed that 46 isolates were of the 4 copy type and 18 isolates were of the 3 copy type. All samples from the eastern coastal area (n = 24) and from the Yucatan peninsula (n = 12) were of the 3 copy type. Six isolates from the west and southwestern area were SNP-type 1, 13 isolates were SNP-type 2 and 45 isolates were SNP-type 3. Nineteen of 24 isolates from the eastern coastal area were SNP-type 3 and one was SNP-type 4. Seven isolates from the Yucatan peninsula were SNP-type 3 and one was SNP-type 4.
  
  
  CONCLUSIONS
  The difference of the proportion of each genotype between the western areas and the eastern areas indicated the expansion of leprosy through different paths in Mexico.
authors: Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1, Aobacho, Higashimurayama-shi, Tokyo, 189-0002, Japan. matsuoka@nih.go.jp
authors: Masanori
authors: M
authors: Matsuoka
authors: Alberto Vargas
authors: AV
authors: Gonzalez
authors: Iris
authors: I
authors: Estrada
authors: Cristina
authors: C
authors: Carreño-Martinez
authors: Mary
authors: M
authors: Fafutis-Morris
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D007918:Leprosy / Q000453:epidemiology / Q000382:microbiology*; D018598:Minisatellite Repeats; D009166:Mycobacterium leprae / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; C414706:rpoT protein, Mycobacterium leprae