Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis.
2-DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V-type ATPase and Deinococcus specific proteins constituting one-fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis.
Liedert
Christina
C
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland. christina.liedert@oulu.fi
Bernhardt
Jörg
J
Albrecht
Dirk
D
Voigt
Birgit
B
Hecker
Michael
M
Salkinoja-Salonen
Mirja
M
Neubauer
Peter
P
D001426:Bacterial Proteins / Q000378:metabolism*; D034301:Deinococcus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D010449:Peptide Mapping; D020543:Proteome / Q000378:metabolism*; D011836:Radiation Tolerance
D001426:Bacterial Proteins; D020543:Proteome