Comparative in vivo expression of amastigote up regulated Leishmania genes in three different forms of Leishmaniasis.
In Old World Leishmania infections in India, Leishmania donovani is responsible for visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) while L. tropica is responsible for cutaneous leishmaniasis (CL) in humans. The molecular differences between the two species of Leishmania and within the same species causing distinct pathologies that govern the outcome of infection and pathogenesis in the human host are unknown. Quantitative expression of selected genes was evaluated directly in lesion tissues of VL, PKDL and CL patients. Assessment of in vivo mRNA level highlighted substantial differences in gene expression patterns, providing an indication of the genes involved in pathogenesis in the three different forms of Leishmaniasis.
Sharma
Paresh
P
Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
Gurumurthy
Srividya
S
Duncan
Robert
R
Nakhasi
Hira L
HL
Salotra
Poonam
P
D000818:Animals; D006801:Humans; D007194:India; D007893:Leishmania donovani / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D007895:Leishmania tropica / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000469:parasitology; D013045:Species Specificity; D015854:Up-Regulation
D015800:Protozoan Proteins; D012333:RNA, Messenger