title: New PI(4,5)P2- and membrane proximal integrin-binding motifs in the talin head control beta3-integrin clustering.
abstract: Integrin-dependent adhesion sites consist of clustered integrins that transmit mechanical forces and provide signaling required for cell survival and morphogenesis. Despite their importance, the regulation of integrin clustering by the cytoplasmic adapter protein talin (Tal) and phosphatidylinositol (PI)-4,5-biphosphate (PI(4,5)P(2)) lipids nor their dynamic coupling to the actin cytoskeleton is fully understood. By using a Tal-dependent integrin clustering assay in intact cells, we identified a PI(4,5)P(2)-binding basic ridge spanning across the F2 and F3 domains of the Tal head that regulates integrin clustering. Clustering requires a new PI(4,5)P(2)-binding site in F2 and is negatively regulated by autoinhibitory interactions between F3 and the Tal rod (Tal-R). The release of the Tal-R exposes a new beta3-integrin-binding site in F3, enabling interaction with a membrane proximal acidic motif, which involves the formation of salt bridges between K(316) and K(324) with E(726) and D(723), respectively. This interaction shields the beta-integrin tail from reassociation with its alpha subunit, thereby maintaining the integrin in a substrate-binding and clustering-competent form.
authors: Department of Cellular Physiology and Metabolism, University Medical Center, University of Geneva, 1211 Geneva 4, Switzerland.
authors: Frédéric
authors: F
authors: Saltel
authors: Eva
authors: E
authors: Mortier
authors: Vesa P
authors: VP
authors: Hytönen
authors: Marie-Claude
authors: MC
authors: Jacquier
authors: Pascale
authors: P
authors: Zimmermann
authors: Viola
authors: V
authors: Vogel
authors: Wei
authors: W
authors: Liu
authors: Bernhard
authors: B
authors: Wehrle-Haller
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D039661:Integrin beta3 / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D016608:Talin / Q000737:chemistry / Q000378:metabolism
chemical_list: D039661:Integrin beta3; D019269:Phosphatidylinositol 4,5-Diphosphate; D016608:Talin