title: Viral infection in bone marrow transplants: is JC virus involved?
abstract: Hemorrhagic cystitis is characterized by hematuria due to inflammation of the bladder. In bone marrow transplants, this disease is linked to the infection by human polyomavirus BK, whereas the role of the human polyomavirus JC is unclear. The transcriptional control regions of both viruses contain important cellular transcription factor binding sites that undergo rearrangement process generating suitable variants that could be more active for viral replication and for the onset of hemorrhagic cystitis. In this study urine obtained from seven patients with bone marrow transplant were examined. Polyomavirus genomes were quantified by PCR and viral loads were compared. The transcriptional regions of both viruses were amplified and sequenced to determine the presence of variants. Subtypes of polyomaviruses were determined by amplification and sequencing of the viral protein 1 region. The results showed that four of seven patients were positive for BK DNA, two of seven patients had BK and JC DNA and one of seven had JC DNA. Positive samples were amplified and sequenced successively for transcriptional regions. The viral archetype was always found in both viruses. Finally, typing showed that BK virus subtype I infected patients with BK, whereas JC virus genotype IA and genotype 1B were found in patients infected with JC. The data suggest that new and different approaches are required to improve the morbidity and mortality caused by polyoma-associated hemorrhagic cystitis, since it known that BK virus is involved in the onset of hemorrhagic cystitis, whereas the role of JC virus should be investigated further.
authors: Department of Public Health Sciences, Sapienza University, Rome, Italy.
authors: Monica
authors: M
authors: Mischitelli
authors: Daniela
authors: D
authors: Fioriti
authors: Elena
authors: E
authors: Anzivino
authors: Anna
authors: A
authors: Bellizzi
authors: Valentina
authors: V
authors: Barucca
authors: Renzo
authors: R
authors: Boldorini
authors: Umberto
authors: U
authors: Miglio
authors: Simona
authors: S
authors: Sica
authors: Federica
authors: F
authors: Sorà
authors: Silvia
authors: S
authors: De Matteis
authors: Fernanda
authors: F
authors: Chiarini
authors: Valeria
authors: V
authors: Pietropaolo
mesh_terms: D000328:Adult; D001739:BK Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D003556:Cystitis / Q000821:virology*; D004279:DNA, Viral / Q000652:urine*; D005260:Female; D006470:Hemorrhage / Q000821:virology*; D006801:Humans; D007558:Italy; D007577:JC Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000821:virology; D014412:Tumor Virus Infections / Q000821:virology; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral