title: Transinfection and growth discrepancy of Drosophila Wolbachia strain wMel in cell lines of the mosquito Aedes albopictus.
abstract: OBJECTIVE
  The Wolbachia strain wMel can protect Drosophila melanogaster against pathogenic RNA viruses. To analyse the potential of this inhibitory effect against arboviruses vectorized by these mosquitoes, we here first transinfected the Aedes albopictus Aa23 and C6/36 cell lines with the Wolbachia strain wMel and then monitored their infection dynamics.
  
  
  RESULTS
  Wolbachia strain wMel was transferred into A. albopictus Aa23 and C6/36 cell lines using the shell vial technique. The presence of the bacterium in the transinfected cells was monitored by quantitative PCR and fluorescence in situ hybridization. Bacteria could be detected in the cytoplasm of both the Aa23 and C6/36 cell lines. However, the dynamics and stability of the bacterial infection differed depending on the initial cell background. The Aa23 cell line, which had been treated with a tetracycline antibiotic 2 years previously to eliminate its natural Wolbachia wAlbB-infecting strain, lost the introduced Wolbachia wMel strain after 12 passages postinfection. In contrast, the C6/36 cell line, which had originally been aposymbiotic, displayed a stable infection with Wolbachia wMel. The bacterial density in C6/36 was greater than that of the A. albopictus RML12 cell line from which the wMel strain had originated.
  
  
  CONCLUSIONS
  Transient or persistent transinfection of A. albopictus Aa23 and C6/36 cell lines with Wolbachia wMel strain was achieved. The results indicate the influence of the genetic background of mosquito cells in maintaining Wolbachia originating from a distant dipteral host.
  
  
  CONCLUSIONS
  The cell model built here can now be used to investigate the viral inhibitory effect of the Wolbachia wMel strain against arboviruses such as dengue and chikungunya, which are transmitted by the mosquito A. albopictus.
authors: Université de Lyon, Lyon, France.
authors: D
authors: D
authors: Voronin
authors: V
authors: V
authors: Tran-Van
authors: P
authors: P
authors: Potier
authors: P
authors: P
authors: Mavingui
mesh_terms: D000330:Aedes / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D003593:Cytoplasm / Q000382:microbiology; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D020577:Wolbachia / Q000254:growth & development* / Q000302:isolation & purification