title: Glucuronic acid and phosphoserine act as mineralization mediators of collagen I based biomimetic substrates.
abstract: Glucuronic acid (GlcA) and phosphoserine (pS) carrying acidic functional groups were used as model molecules for glycosaminoglycans and phosphoproteins, respectively to mimic effects of native biomolecules and influence the mineralization behaviour of collagen I. Collagen substrates modified with GlcA showed a stable interaction between GlcA and collagen fibrils. Substrates were mineralized using the electrochemically assisted deposition (ECAD) in a Ca(2+)/H( x )PO (4) ((3-x)) electrolyte at physiological pH and temperature. During mineralization of collagen-GlcA matrices, crystalline hydroxyapatite (HA) formed earlier with increasing GlcA content of the collagen matrix, while the addition of pS to the electrolyte succeeded in inhibiting the transformation of preformed amorphous calcium phosphate (ACP) to HA. The lower density of the resulting mineralization and the coalesced aggregates formed at a certain pS concentration suggest an interaction between calcium and the phosphate groups of pS involving the formation of complexes. Combining GlcA-modified collagen and pS-modified electrolyte showed dose-dependent cooperative effects.
authors: Max Bergmann Center of Biomaterials, Dresden University of Technology, Budapester Str. 27, 01069, Dresden, Germany. ricardo@minin.es
authors: Ricardo
authors: R
authors: Tejero
authors: Susanne
authors: S
authors: Bierbaum
authors: Timothy
authors: T
authors: Douglas
authors: Antje
authors: A
authors: Reinstorf
authors: Hartmut
authors: H
authors: Worch
authors: Dieter
authors: D
authors: Scharnweber
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D020723:Glucuronic Acid / Q000737:chemistry*; D008422:Materials Testing; D008903:Minerals / Q000737:chemistry*; D010768:Phosphoserine / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D024042:Collagen Type I; D008903:Minerals; D010768:Phosphoserine; D020723:Glucuronic Acid