title: Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation.
abstract: Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of PDB-MSCs in hypoxia and serum deprivation. PDB-MSCs were cultured in four groups: normoxia (20% O2) and complete medium [10% FBS (foetal bovine serum)+DMEM-HG (Dulbecco's modified Eagle's medium-high glucose)], hypoxia and complete medium, normoxia and serum deprivation (0% FBS), and hypoxia and serum deprivation. After 96 h of culture in the above groups, PDB-MSCs maintain the phenotypes stably. Interestingly, hypoxia notably enhanced the proliferation, colony-forming potential and lactate/glucose ratio in complete medium, but suppressed the secretion of BMP-2 (bone morphogenetic protein-2) and bFGF (basic fibroblast growth factor), while it did not change the quantity of VEGF (vascular endothelial growth factor) and bFGF in serum deprivation. Although PDB-MSCs grew slowly and seldom formed a colony unit in hypoxia and serum deprivation, they possessed a moderate metabolism. In conclusion, our results indicate that PDB-MSCs appear to be promising seed cells for ischaemia-related tissue engineering.
authors: Division of Stem cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Yong-Can
authors: YC
authors: Huang
authors: Zhi-Ming
authors: ZM
authors: Yang
authors: Neng-Gang
authors: NG
authors: Jiang
authors: Xiao-He
authors: XH
authors: Chen
authors: Xiu-Qun
authors: XQ
authors: Li
authors: Mei-Yun
authors: MY
authors: Tan
authors: Kun-Peng
authors: KP
authors: Zhou
authors: Li
authors: L
authors: Tang
authors: Hui-Qi
authors: HQ
authors: Xie
authors: Li
authors: L
authors: Deng
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D015687:Cell Hypoxia; D049109:Cell Proliferation; D016895:Culture Media, Serum-Free; D003656:Decidua / Q000166:cytology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D016895:Culture Media, Serum-Free; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2