Immunogenicity and efficacy of single antigen Gp63, polytope and polytopeHSP70 DNA vaccines against visceral Leishmaniasis in experimental mouse model.
Polytope approach of genetic immunization is a promising strategy for the prevention of infectious disease as it is capable of generating effective cell mediated immunity by delivering the T cell epitopes assembled in series. Leishmaniasis is a significant world wide health problem for which no vaccine exists. In this study we have compared immunogenicity and efficacy of three types of DNA vaccines: single antigen Gp63 (Gp63/pcDNA), polytope (Poly/pcDNA) and Polytope fused with hsp70 (Poly/hsp/pcDNA) against visceral leishmaniasis in susceptible BALB/c mice. Mice vaccinated with these plasmids generated strong Th1 immune response as seen by dominating IFN-gamma over IL-10 cytokine. Interestingly, cytotoxic responses generated by polytope DNA plasmid fused with hsp70 of Leishmania donovani were significantly higher when compared to polytope and single antigen Gp63 vaccine. Challenge studies revealed that the parasite load in liver and spleen was significantly lower with Poly/hsp/pcDNA vaccination compared to other vaccines. Therefore, our study indicates that polytope DNA vaccine is a feasible, practical and effective approach for visceral leishmaniasis.
Sachdeva
Rakhee
R
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Banerjea
Akhil C
AC
Malla
Nancy
N
Dubey
Mohan Lal
ML
D000818:Animals; D016923:Cell Death; D049109:Cell Proliferation; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology; D007118:Immunoassay; D007898:Leishmaniasis, Visceral / Q000139:chemically induced / Q000276:immunology* / Q000469:parasitology / Q000628:therapy*; D008099:Liver / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008666:Metalloendopeptidases / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D016896:Treatment Outcome; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D016207:Cytokines; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins; D019444:Vaccines, DNA; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania