HPLC analysis of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes activity and Bax, Bcl-2 expression.
The polysaccharides from Ganoderma lucidum (GLP) were prepared using boiling water extraction methods. High performance liquid chromatography (HPLC) showed that GLP was composed of five monosaccharides and disaccharides, including xylose, fructose, glucose, sucrose and maltose, were identified for polysaccharides from Ganoderma lucidum. The relative molar percentages of xylose, fructose, glucose, sucrose and maltose in polysaccharides from Ganoderma lucidum were 0.4%, 14.4%, 12.8%, 0.7% and 50.9%, respectively. GLP (100, 200, 300mg/kg) was administered to diabetic rats. Effect of extract on antioxidant enzymes activities and lipid peroxidation levels of pancreas was studied in diabetic rats. The mechanism of anti-apoptosis of GLP in STZ-induced diabetic rats was further investigated using Bax/Bcl-2 ratio and protein. Result showed that GLP extracts effectively reduced oxidative injury and inhibited apoptosis by increasing antioxidant enzyme activities and modifying bcl-2 expression and bax/bcl-2 ratio. The GLP extract exhibited potent antioxidant effect in vivo. The effect can contribute, at least in part, to the inhibition of apoptosis.
Yang
Qian
Q
Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
Wang
SiWang
S
Xie
YanHua
Y
Sun
JiYuan
J
Wang
JianBo
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000187:drug effects; D015153:Blotting, Western; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D011134:Polysaccharides / Q000032:analysis* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D020940:Reishi / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D001786:Blood Glucose; D011134:Polysaccharides; D012333:RNA, Messenger; D014280:Triglycerides; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D008315:Malondialdehyde; D002784:Cholesterol; D005978:Glutathione