Lensless imaging for point-of-care testing.
We show a platform that merges a microfluidic chip with lensless imaging for CD4(+) T-lymphocyte counting at resource-limited settings. To capture CD4(+) T lymphocytes, anti-CD4 antibody was immobilized on a microfluidic chip. The captured cells were detected by a charge coupled device (CCD) sensor using lensless shadow imaging techniques. Gray scale shadow images of captured cells on the chip (24 mm x 4 mm x 50 mum) were enumerated in three seconds using an automatic cell counting software. The device achieved 70.2 +/- 6.5% capture efficiency, 88.8 +/- 5.4% capture specificity for CD4(+) T-lymphocytes, 96 +/- 1.6% CCD efficiency, and 83.5 +/- 2.4% overall platform performance (n = 9 devices). This integrated platform has potential for point-of-care testing (POCT) to rapidly capture, image and count specific cell types from unprocessed whole blood.
Moon
SangJun
S
Bio-Acoustic-MEMS in Medicine Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Keles
Hasan Onur
HO
Kim
Yun-Gon
YG
Kuritzkes
Daniel
D
Demirci
Utkan
U
D018791:CD4 Lymphocyte Count / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007909:Lenses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055096:Optical Devices; D010783:Photometry / Q000295:instrumentation*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*