Bacterial microbiota profiling in gastritis without Helicobacter pylori infection or non-steroidal anti-inflammatory drug use.
Recent 16S ribosomal RNA gene (rRNA) molecular profiling of the stomach mucosa revealed a surprising complexity of microbiota. Helicobacter pylori infection and non-steroidal anti-inflammatory drug (NSAID) use are two main contributors to gastritis and peptic ulcer. However, little is known about the association between other members of the stomach microbiota and gastric diseases. In this study, cloning and sequencing of the 16S rRNA was used to profile the stomach microbiota from normal and gastritis patients. One hundred and thirty three phylotypes from eight bacterial phyla were identified. The stomach microbiota was found to be closely adhered to the mucosa. Eleven Streptococcus phylotypes were successfully cultivated from the biopsies. One to two genera represented a majority of clones within any of the identified phyla. We further developed two real-time quantitative PCR assays to quantify the relative abundance of the Firmicutes phylum and the Streptococcus genus. Significantly higher abundance of the Firmicutes phylum and the Streptococcus genus within the Firmicutes phylum was observed in patients with antral gastritis, compared with normal controls. This study suggests that the genus taxon level can largely represent much higher taxa such as the phylum. The clinical relevance and the mechanism underlying the altered microbiota composition in gastritis require further functional studies.
Li
Xiao-Xing
XX
Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
Wong
Grace Lai-Hung
GL
To
Ka-Fai
KF
Wong
Vincent Wai-Sun
VW
Lai
Larry Hin
LH
Chow
Dorothy Kai-Lai
DK
Lau
James Yun-Wong
JY
Sung
Joseph Jao-Yiu
JJ
Ding
Chunming
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D001706:Biopsy; D004724:Endoscopy; D005260:Female; D005756:Gastritis / Q000175:diagnosis / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012336:RNA, Ribosomal, 16S