Inhibition property of green tea extract in relation to reserpine-induced ribosomal strips of rough endoplasmic reticulum (rER) of the rat kidney proximal tubule cells.
The aim of this study was to evaluate the effect of green tea in inhibiting and reversing the nephrotoxicity of reserpine--a potent oxidative stress inducer--which induced cellular kidney damage. Serum biochemical parameters, antioxidant enzyme levels, thiobarbituric acid reactive substances (TBARS) and serum transaminases (glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT)) values and histopathology were systematically evaluated. Reserpine exposure led to increase the oxidative stress and organ injury was significantly observed through biochemical parameters and ultrastructural evaluation. Sprague-Dawely (S.D.) rats were intraperitonealy administered reserpine to induce oxidative kidney damage. Experimental rats were given green tea extract according to the protocol given below. Sixty rats were randomly divided into six groups, with 10 rats in each group. Reserpine was found to cause kidney proximal tubule damage, such as stripping and clustering of ribosomes from the rough endoplasmic reticulum (rER) and demolishing of mitochondrial christae with elevated level of oxidative stress markers, such as TBARS. While the ultrastructural study showed a revival of kidney proximal tubule cells as a result of the administration of green tea extract to rats. We suggest that green tea might elevate antioxidant defense system, clean up free radicals, lessen oxidative damages and protect kidney against reserpine-induced toxicity and thus had a potential protective effect.
Abdel-Majeed
Safer
S
Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait. amsafer79@yahoo.com
Mohammad
Afzal
A
Shaima
Al-Bloushi
AB
Mohammad
Rafique
R
Mousa
Shaker A
SA
D000818:Animals; D028241:Camellia sinensis; D018870:Endoplasmic Reticulum, Rough / Q000473:pathology* / Q000648:ultrastructure; D007674:Kidney Diseases / Q000139:chemically induced* / Q000517:prevention & control*; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000633:toxicity*; D012270:Ribosomes / Q000473:pathology* / Q000648:ultrastructure; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D010936:Plant Extracts; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D012110:Reserpine