Cytoskeletal changes during cellular response of the A549 lung cancer cells to continuous cisplatin treatment.
The cytoskeleton is a ubiquitous cellular structure that plays a crucial role in most processes of living cells. There are reports suggesting that this system not only reflects, but also contributes to many different processes, including cell death. In this study, we examined alterations of both MT and MF cytoskeletal systems related to cell death, which was induced in A549 cells by continuous cisplatin treatment. We observed that specific changes in these cytoskeletal proteins accompany cell death, while the others are associated with increased repair and cell survival. It seems that the predominant mode of cell death triggered by cisplatin was an apoptotic-like pathway, but on the other hand, coincidence with some features of necrosis and autophagy was also demonstrated in our conditions.
Szczepański
Mariusz Andrzej
MA
Department of Histology and Embryology, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University, Karlowicza 24, 85-092 Bydgoszcz, Poland. mariusz.szczepanski@cm.umk.pl
Grzanka
Alina
A
Litwiniec
Anna
A
Gackowska
Lidia
L
Kubiszewska
Izabela
I
Grzanka
Dariusz
D
D000199:Actins / Q000378:metabolism; D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D001343:Autophagy; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000648:ultrastructure*; D009336:Necrosis; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D017304:Annexin A5; D000970:Antineoplastic Agents; D014404:Tubulin; D002945:Cisplatin