Role of Saccharomyces cerevisiae oxidoreductases Bdh1p and Ara1p in the metabolism of acetoin and 2,3-butanediol.
NAD-dependent butanediol dehydrogenase (Bdh1p) from Saccharomyces cerevisiae reversibly transforms acetoin to 2,3-butanediol in a stereospecific manner. Deletion of BDH1 resulted in an accumulation of acetoin and a diminution of 2,3-butanediol in two S. cerevisiae strains under two different growth conditions. The concentrations of (2R,3R)-2,3-butanediol are mostly dependent on Bdh1p activity, while those of (meso)-2,3-butanediol are also influenced by the activity of NADP(H)-dependent oxidoreductases. One of them has been purified and shown to be d-arabinose dehydrogenase (Ara1p), which converts (R/S)-acetoin to meso-2,3-butanediol and (2S,3S)-2,3-butanediol. Deletion of BDH2, a gene adjacent to BDH1, whose encoded protein is 51% identical to Bdh1p, does not significantly alter the levels of acetoin or 2,3-butanediol in comparison to the wild-type strain. Furthermore, we have expressed Bdh2p with a histidine tag and have shown it to be inactive toward 2,3-butanediol. A whole-genome expression analysis with microarrays demonstrates that BDH1 and BDH2 are reciprocally regulated.
González
Eva
E
Department of Biochemistry and Molecular Biology, Faculty of Biosciences, Universitat Autònoma de Barcelona, Barcelona, Spain.
Fernández
M Rosario
MR
Marco
Didac
D
Calam
Eduard
E
Sumoy
Lauro
L
Parés
Xavier
X
Dequin
Sylvie
S
Biosca
Josep A
JA
D000093:Acetoin / Q000378:metabolism*; D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019943:Amino Acid Substitution; D000693:Anaerobiosis; D002072:Butylene Glycols / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005285:Fermentation; D017353:Gene Deletion; D005818:Genetic Engineering; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism
D002072:Butylene Glycols; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; C026978:2,3-butylene glycol; D000093:Acetoin; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; C028590:D-arabinose dehydrogenase; C019801:butanediol dehydrogenase