C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens.
We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.
Quinn
Criziel D
CD
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Sefers
Susan E
SE
Babiker
Wisal
W
He
Ying
Y
Alcabasa
Romina
R
Stratton
Charles W
CW
Carroll
Karen C
KC
Tang
Yi-Wei
YW
D001399:Azure Stains / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D016360:Clostridioides difficile / Q000737:chemistry / Q000302:isolation & purification*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis*; D004768:Enterotoxins / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D005969:Glutamate Dehydrogenase / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008751:Methylene Blue / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014966:Xanthenes / Q000378:metabolism*
D001399:Azure Stains; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C488583:Clostridium difficile lethal toxin B; C067461:Diff Quik; D004768:Enterotoxins; D014966:Xanthenes; C041348:tcdA protein, Clostridium difficile; D005969:Glutamate Dehydrogenase; D008751:Methylene Blue