title: Arginine vasopressin induces rat caudate nucleus releasing acetylcholine to participate in pain modulation.
abstract: A lot of studies have pointed that acetylcholine (Ach), a classic neurotransmitter can regulate pain process in the caudate nucleus (CdN). Our previous report has proven that arginine vasopressin (AVP) effects on pain modulation in the CdN. The communication was designed to investigate the interaction between AVP and Ach in the rat CdN during the pain process. AVP concentration was determined by radioimmunoassay (RIA) and Ach concentration by high performance liquid chromatography (HPLC). The results showed that pain stimulation increased both AVP and Ach concentrations in the CdN perfusion liquid; AVP increased Ach concentration in the CdN perfusion liquid, while AVP receptor antagonist including d(CH(2))(5)Tyr(Me)AVP (V(1) receptor antagonist) and d(CH(2))(5)[D-Ile(2), Ile(4), Ala-NH(2)(9)]AVP (V(2) receptor antagonist) decreased Ach concentration in the CdN perfusion liquid. The data indicated that the analgesic effect of AVP might be involved in the Ach system in the CdN.
authors: Clinical Medical College of Yangzhou University (Subei People's Hospital), Yangzhou, Jiangsu 225001, China.
authors: Da-Xin
authors: DX
authors: Wang
authors: Jun
authors: J
authors: Yang
authors: Zhi-Xin
authors: ZX
authors: Gu
authors: Chao-You
authors: CY
authors: Song
authors: Wen-Yan
authors: WY
authors: Liu
authors: Jing
authors: J
authors: Zhang
authors: Xue-Ping
authors: XP
authors: Li
authors: Hui
authors: H
authors: Li
authors: Gen
authors: G
authors: Wang
authors: Cai
authors: C
authors: Song
authors: Bao-Cheng
authors: BC
authors: Lin
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002421:Caudate Nucleus / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007276:Injections, Intraventricular; D008297:Male; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D065092:Antidiuretic Hormone Receptor Antagonists; D001127:Arginine Vasopressin; D000109:Acetylcholine