No association of the IRS1 and PAX4 genes with type I diabetes.
To reassess earlier suggested type I diabetes (T1D) associations of the insulin receptor substrate 1 (IRS1) and the paired domain 4 gene (PAX4) genes, the Type I Diabetes Genetics Consortium (T1DGC) evaluated single-nucleotide polymorphisms (SNPs) covering the two genomic regions. Sixteen SNPs were evaluated for IRS1 and 10 for PAX4. Both genes are biological candidate genes for T1D. Genotyping was performed in 2300 T1D families on both Illumina and Sequenom genotyping platforms. Data quality and concordance between the platforms were assessed for each SNP. Transmission disequilibrium testing neither show T1D association of SNPs in the two genes, nor did haplotype analysis. In conclusion, the earlier suggested associations of IRS1 and PAX4 to T1D were not supported, suggesting that they may have been false positive results. This highlights the importance of thorough quality control, selection of tagging SNPs, more than one genotyping platform in high throughput studies, and sufficient power to draw solid conclusions in genetic studies of human complex diseases.
Bergholdt
R
R
Hagedorn Research Institute and Steno Diabetes Center, Niels Steensens Vej 1, Gentofte, Denmark.
Brorsson
C
C
Boehm
B
B
Morahan
G
G
Pociot
F
F
D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D051761:Paired Box Transcription Factors / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
D018398:Homeodomain Proteins; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C491709:PAX4 protein, human; D051761:Paired Box Transcription Factors