Identification of sugar signals controlling the nitrate uptake by rice roots using a noninvasive technique.
In order to evaluate the hypothesis that nitrate uptake is under positive control by sugars, a simple noninvasive model was used to measure the effects of nine modulating sugars on the net nitrate uptake in rice under constant low nitrate concentration. The results showed that the fastest and greatest acceleration in nitrate uptake were observed after sucrose was added, and this change reached a peak 1.5 h after treatment. In addition, we found that 1 mM sucrose could affect the nitrate uptake in rice roots for 8.5 h. The three most positive effective sugars and one negative effective sugar were selected for further analyzing their effect on the expression of nitrate transporter gene OsNrt2.1. The result of RT-PCR showed that the expression of OsNrt2.1 was upregulated by sucrose, glucose and galactose. Among the three positive effective sugars tested, sucrose was found to have significant and continuous enhanced stimulation on OsNrt2.1 gene expression within 4 h, which indicated that sucrose could be as a specific signal to regulate the net nitrate uptake.
Zhou
Shiyi
S
China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International Cooperation Base of Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Luoyu Road 1037, Wuhan 430074, China.
Gao
Xuan
X
Wang
Changdong
C
Yang
Guangxiao
G
Cram
William John
WJ
He
Guangyuan
G
D009566:Nitrates / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009566:Nitrates