Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development.
Recent studies have shown that short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos. The aim of the present study was to investigate the consequences of short-term hydrogen peroxide (H(2)O(2)) exposure to bovine in vitro matured cumulus-oocyte complexes (COCs) on subsequent preimplantation embryo development and apoptosis. In the first experiment, mature COCs were incubated in H(2)O(2) at concentrations ranging between 0.01 and 100 micromol/l, and subsequently fertilized and cultured. Oocyte incubation with 50-100 micromol/l of H(2)O(2) resulted in a significantly higher blastocyst yield (47.3%) in comparison with control medium (31.8%), while apoptotic cell ratio was inversely related with H(2)O(2) concentration. In the second experiment, we showed that the stress tolerance after H(2)O(2) exposure was not mediated by increased glutathione content in treated oocytes nor by enhanced fertilization or penetration. Further research should concentrate on the potential role of players that have been associated with stress tolerance in somatic cell lines.
Vandaele
L
L
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. leen.vandaele@ugent.be
Thys
M
M
Bijttebier
J
J
Van Langendonckt
A
A
Donnay
I
I
Maes
D
D
Meyer
E
E
Van Soom
A
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002417:Cattle / Q000196:embryology / Q000502:physiology; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D006861:Hydrogen Peroxide; D005978:Glutathione