High-efficient FLPo deleter mice in C57BL/6J background.
Conditional gene manipulation in mice becomes a routine for genetic studies of mammalian gene functions. Additional site-specific recombinases such as FLP or phi31 provide one more level of gene manipulation flexibility. The recombination activity of the currently available FLP deleter mice remains low. We generated a new FLP deleter mouse line with the mouse codon-optimized FLPo gene in C57BJ/6 background, which showed superior recombination efficacy in comparison to FLPe deleter mice. 100% complete removal of FRT-flanked Neo cassette was observed in all F1 progeny mice carrying both FLPo and Neo cassette, which can be transmitted to F2 generation independent of FLPo activity. Our new FLPo transgenic mice (on pure C57BJ/6 background) will largely facilitate the gene targeting process and is valuable for conditional gene manipulation.
Wu
Yingjie
Y
Endocrinology/Diabetes and Bone Disease Division, Mount Sinai School of Medicine, New York, New York, United States of America.
Wang
Chunxin
C
Sun
Hui
H
LeRoith
Derek
D
Yakar
Shoshana
S
D000818:Animals; D003433:Crosses, Genetic; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D017353:Gene Deletion; D018390:Gene Targeting; D005818:Genetic Engineering; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase