[Inhibitory effect of lentivirus transduced anti-telomerase siRNA therapy on hepatocellular carcinoma in nude mice].
OBJECTIVE
To study the efficacy of anti-telomerase siRNA in hepatocellular carcinoma both in vitro and in vivo.


METHODS
Lentvirus vectors contained anti-telomerase siRNA were conducted with a high performance homologous recombination system, and then were transduced into human hepatocellular carcinoma HepG2 cells. The telomerase activity was detected by RT-PCR, HepG2 cell proliferation was determined by MTT assay, and apoptosis was detected by TUNEL assay. The in vivo experiment was carried out by inoculation of HepG2 cells into nude mice and the tumor growth was measured and analyzed.


RESULTS
The growth of transfected HepG2 cells was significantly inhibited and the inhibition rate was 57.5% at the 8th day after transfection. The telomerase activity was significantly suppressed in vitro. The growth of transfected human hepatocellular HepG2 tumor in the nude mice was also significantly inhibited.


CONCLUSIONS
The results of this study demonstrate that the growth of hepatocellular carcinoma cells is effectively inhibited by transfection of anti-telomerase siRNA both in vitro and in vivo.
Zhang
Yong
Y
Liver Transplantation Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Gao
Yun
Y
Xi
Li-Sen
LS
Deng
Lei
L
Yin
Ai-Hong
AH
Wang
Xue-Hao
XH
Sun
Bei-Cheng
BC
D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000628:therapy; D049109:Cell Proliferation; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008113:Liver Neoplasms / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D047368:Tumor Burden
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C509186:TERT protein, human; D019098:Telomerase