Investigation of the immunosuppressive activity of Physalin H on T lymphocytes.
Physalis angulata is an annual herb widely used in folk medicine. It is mainly used for treating rheumatoid arthritis (RA). Following bioactivity-guided isolation, a representative immunosuppressive compound, Physalin H was been identified from this herb medicine. The purpose of this work was to assess the immunosuppressive activity of Physalin H on T cells and to explore its potential mode of action. The results showed that Physalin H in a dose-dependent manner significantly inhibited the proliferation of T cells induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR). This inhibitive activity was mainly due to interfering DNA replication in G1 stages. In vivo experiments showed that, administration of Physalin H dose-dependently suppressed CD4(+) T cell mediated delayed-type hypersensitivity (DTH) reactions, and suppressed antigen-specific T-cell response in ovalbumin (OVA) immunized mice. Further study indicated that Physalin H could modulate Th1/Th2 cytokine balance and induce the production of immune regulation target Heme oxygenase (HO)-1 in T-cells in vitro. In this study, we demonstrated the immunosuppressive effect of Physalin H on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells.
Yu
Youjun
Y
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Sun
Lijuan
L
Ma
Lei
L
Li
Jiyu
J
Hu
Lihong
L
Liu
Jianwen
J
D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D003208:Concanavalin A / Q000494:pharmacology; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012632:Secosteroids / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D012632:Secosteroids; D013778:Tetrazolium Salts; D013844:Thiazoles; D003208:Concanavalin A; C475936:physalin H; D010047:Ovalbumin; D004139:Dinitrofluorobenzene; D051547:Heme Oxygenase-1; C022616:thiazolyl blue