Hydroxyproline-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate.
Several hydroxyproline (Hyp)-containing food-derived collagen peptides were identified in human blood after oral ingestion of gelatin hydrolysates. However, these types of peptides were not quantified in human plasma. In this report, a sensitive LC-MS/MS method was introduced for simultaneous quantitative analysis of Hyp-containing peptides. All peptide concentrations were determined accurately, with all coefficients of determination (r(2)) >0.999. The method achieved detection and quantification limits of 0.01 pmol/ml and 12.5-1,000 pmol/ml in plasma, respectively. Concentrations were quantified for nine Hyp-containing peptides in human plasma by this method, identifying Pro-Hyp (C(max) = 60.65 +/- 5.74 nmol/ml) as the major constituent of food-derived collagen peptides, while the minor components were Ala-Hyp-Gly, Ser-Hyp-Gly, Ala-Hyp, Phe-Hyp, Leu-Hyp, Ile-Hyp, Gly-Pro-Hyp, and Pro-Hyp-Gly (C(max) from 23.84 to 0.67 nmol/ml). Thus a total of nine Hyp-containing peptides in human plasma were successfully quantified by this approach. The concentration of Hyp-containing peptides is substantially higher than that following oral administration of other peptides.
Ichikawa
Satomi
S
Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd, Sakado-shi, Saitama, Japan.
Morifuji
Masashi
M
Ohara
Hiroki
H
Matsumoto
Hitoshi
H
Takeuchi
Yasuo
Y
Sato
Kenji
K
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000008:administration & dosage*; D004151:Dipeptides / Q000097:blood; D005780:Gelatin / Q000008:administration & dosage*; D006801:Humans; D006868:Hydrolysis; D006909:Hydroxyproline / Q000097:blood*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000097:blood*
D004151:Dipeptides; D010446:Peptide Fragments; D010455:Peptides; D005780:Gelatin; D003094:Collagen; D006909:Hydroxyproline