[Purification of total paeony glycoside by macroporous resin with double indices of albiflorin and paeoniflorin].
OBJECTIVE
To investigate the purification process of total paeony glycoside (TPG) from Radix Paeoniae Rubra by macroporous resin with double indices of albiflorin and paeoniflorin.


METHODS
HPLC was used for simultaneous determination of albiflorin and paeoniflorin and the results were used as main indices to investigate the technological parameters for purifying TPG by D101 macroporous resin. Then the result was verified by ampliate test.


RESULTS
The applicable technological conditions of purification for TPG by D101 macroporous resin were as follows: the concentrations of albiflorin and paeoniflorin in original solution were 0.12 mg/mL, 2.58 mg/mL with a flow rate of 3.6 BV/h, and the adsorption capacity were 0.71 mg/mL, 15.43 mg/mL, respectively. The eluant was 20% alcohol with 8 times of resin volume, and the elution ratio were 72.18%, 90.84%, respectively. The result of ampliate test accorded with that of small test.


CONCLUSIONS
D101 macroporous adsorption resin can be used to purify TPG from crude extracts of Radix Paeoniae Rubra.
Dou
Zhi-Hua
ZH
Nantong Third Affiliated Hospital of Nantong University, Nantong 226006, China. zhihuadou@163.com
Luo
Lin
L
Lu
Dan
D
D000042:Absorption; D001565:Benzoates / Q000032:analysis / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; D012117:Resins, Synthetic; C015423:peoniflorin; C014959:albiflorin; D000431:Ethanol