title: [Transbronchial lung biopsy using cryoprobes].
abstract: OBJECTIVE
  Transbronchial lung biopsy (TBLB) is a bronchoscopy procedure used to obtain peripheral lung tissue. Small size samples and artefacts lead to variable, and usually poor, diagnostic yield. The use of cryoprobes may enable larger size and better quality biopsy samples to be obtained. The purpose of this study was to evaluate the feasibility of TBLB with cryoprobes and analyse the histological quality of samples obtained.
  
  
  METHODS
  We selected 10 patients with interstitial lung disease who were suitable for TBLB. A cryoprobe (Erbokryo CA, Erbe, Germany) was introduced through the bronchoscope work channel. Then, under fluoroscopic control, the cryoprobe was placed in an area of the peripheral lung previously selected according to CT findings. A temperature of -89.5 degrees C was applied for 3s and the cryoprobe and bronchoscope were removed with the frozen lung sample attached to the probe. The procedure was performed under sedation and the patient was intubated to allow bronchoscope and cryoprobe removal. Safety, duration of the procedure and histological findings has been evaluated.
  
  
  RESULTS
  There were 10 patients (64+/-8 years, 6 males). Procedure length was 35 min. The specimen area was 9.5 mm2 (range 3 to 25 mm2) and the mean number of alveolar spaces was 29.62. No pneumothorax was registered. 6/10 patients had mild post-biopsy bleeding controlled with standard bronchoscopy measures.
  
  
  CONCLUSIONS
  The use of cryoprobes for TBLB may become an alternative technique to increase diagnostic yield.
authors: Unidad de Broncología, Departamento de Neumología, Hospital de Santa Creu i Sant Pau, Universidad Autónoma de Barcelona, Barcelona, España. vpajares@santpau.cat
authors: Virginia
authors: V
authors: Pajares
authors: Alfons
authors: A
authors: Torrego
authors: Carmen
authors: C
authors: Puzo
authors: Enrique
authors: E
authors: Lerma
authors: Maria Angels
authors: MA
authors: Gil De Bernabé
authors: Tomás
authors: T
authors: Franquet
mesh_terms: D001706:Biopsy; D001984:Bronchial Neoplasms / Q000473:pathology; D001999:Bronchoscopy / Q000379:methods*; D015925:Cryopreservation / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D006801:Humans; D008168:Lung / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies