title: SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells.
abstract: Despite the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we investigated whether skin-derived precursors (SKPs) might fulfill such a role. We show that SKPs derive from Sox2(+) hair follicle dermal cells and that these two cell populations are similar with regard to their transcriptome and functional properties. Both clonal SKPs and endogenous Sox2(+) cells induce hair morphogenesis, differentiate into dermal cell types, and home to a hair follicle niche upon transplantation. Moreover, hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. Finally, grafting experiments show that follicle-associated dermal cells move out of their niche to contribute cells for dermal maintenance and wound-healing. Thus, SKPs derive from Sox2(+) follicle-associated dermal precursors and display functional properties predicted of a dermal stem cell, contributing to dermal maintenance, wound-healing, and hair follicle morphogenesis.
authors: Hospital for Sick Children, University of Toronto, ON, Canada. jeff.biernaskie@ucalgary.ca
authors: Jeffrey
authors: J
authors: Biernaskie
authors: Maryline
authors: M
authors: Paris
authors: Olena
authors: O
authors: Morozova
authors: B Matthew
authors: BM
authors: Fagan
authors: Marco
authors: M
authors: Marra
authors: Larysa
authors: L
authors: Pevny
authors: Freda D
authors: FD
authors: Miller
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D009024:Morphogenesis; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis*; D055153:Stem Cell Niche / Q000166:cytology*; D033581:Stem Cell Transplantation; D014945:Wound Healing
chemical_list: D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse