FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1).
In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.
Chen
Chun-Yu
CY
Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi, Taiwan.
Lai
Ning-Sheng
NS
Yang
Jaw-Ji
JJ
Huang
Hsien-Lu
HL
Hung
Wei-Chuan
WC
Li
Chin
C
Lin
Ta-Hsien
TH
Huang
Hsien-Bin
HB
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D015513:Oncogene Proteins; D054645:Protein Phosphatase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D015513:Oncogene Proteins; D011994:Recombinant Proteins; C588270:SH3RF2 protein, human; D044767:Ubiquitin-Protein Ligases; D054645:Protein Phosphatase 1