Salicylic acid-altering arabidopsis mutants response to NO(2) exposure.
Nitrogen dioxide (NO(2))-induced responses in wild type (wt) and salicylic acid (SA)-altering Arabidopsis mutants snc1 (suppressor of npr1-1, constitutive) with high SA level, transgenic line nahG with low SA level, npr1-1 (nonexpressor of PR gene) with SA signaling blockage and double mutant snc1nahG plants, were investigated. All mutant lines except sncl showed that NO(2) exposure at 0.25 microL L(-1) increased chlorophyll content and biomass accumulation, elevated photosynthetic rate, and decreased MDA content compared to their respective controls. The sncl plants were similar to the control plants for these measured indices. NO(2) exposure at 0.5 microL L(-1) and higher doses caused injury to wt, nahG, npr1-1 and snc1nahG plants, whereas the snc1 plants exhibited a stronger tolerance. To evaluate the resistance mechanism, we further investigated the changes in the mutants exposed to 1 microL L(-1) of NO(2) in relation to endogenous SA level, antioxidant capacity and redox status. The collected data demonstrated that the NO(2) tolerance in snc1, with a high SA level, was tightly linked to the increased antioxidant capacity and decreased oxidative stress. This suggests that SA may play an important protective function in plant response to NO(2) stress.
Xu
Qiang
Q
College of Life and Chemistry Sciences, Shenyang Normal University, 110034, Shenyang, People's Republic of China.
Zhou
Bing
B
Ma
Chunyan
C
Xu
Xin
X
Xu
Jia
J
Jiang
Yangao
Y
Liu
Chang
C
Li
Guangzhe
G
Herbert
Stephen J
SJ
Hao
Lin
L
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D002734:Chlorophyll / Q000378:metabolism; D009154:Mutation; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D018384:Oxidative Stress; D010788:Photosynthesis / Q000187:drug effects; D030821:Plants, Genetically Modified / Q000187:drug effects; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction
D002734:Chlorophyll; D020156:Salicylic Acid; D009585:Nitrogen Dioxide