title: Influence of membrane CD25 stability on T lymphocyte activity: implications for immunoregulation.
abstract: BACKGROUND
  CD25, a component of the IL-2 receptor, is important in T cell proliferation, activation induced cell death, as well as the actions of both regulatory (Treg) and effector (Teff) T cells. Recent genome wide association studies have implicated the CD25 locus as an important region for genetic susceptibility to a number of autoimmune disorders, with serum levels of soluble CD25 receptor (sCD25) serving as a potential phenotypic marker for this association. However, the functional impact of CD25 cleavage, as well as the influence of sCD25 on immunoregulatory activities, remain largely unknown and form the basis of this effort.
  
  
  RESULTS
  The generation of sCD25 by Treg (CD4(+)CD25(+)) and Teff (CD4(+)CD25(-)) cells was examined during in vitro suppression assays, efforts that demonstrated constitutive and stable surface CD25 expression on Treg throughout the period of in vitro assessment. In contrast, Teff cells increased CD25 expression during the process of in vitro suppression, with supernatant sCD25 levels correlating to the amount of cellular proliferation. Interestingly, under serum-free conditions, Tregs partially lost their characteristic anergic and suppressive properties. sCD25 supplementation at physiological concentrations to serum free in vitro suppression assays reduced Teff proliferation without specifically influencing suppression. Indeed, sCD25 production within these cultures correlated with cell death.
  
  
  CONCLUSIONS
  These results support the notion that sCD25 functions as both a surrogate marker of T cell activation as well as an indicator of subsequent cellular death. In addition, the role of CD25 in immunomodulation is likely dependent on the local inflammatory milieu, with molecules capable of modulating surface CD25 expression playing a key role in defining immune responsiveness.
authors: Department of Pathology, University of Florida, Gainesville, Florida, United States of America.
authors: Todd M
authors: TM
authors: Brusko
authors: Clive H
authors: CH
authors: Wasserfall
authors: Maigan A
authors: MA
authors: Hulme
authors: Roniel
authors: R
authors: Cabrera
authors: Desmond
authors: D
authors: Schatz
authors: Mark A
authors: MA
authors: Atkinson
mesh_terms: D000328:Adult; D001327:Autoimmune Diseases / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007107:Immune System / Q000502:physiology*; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit