Alkalibaculum bacchi gen. nov., sp. nov., a CO-oxidizing, ethanol-producing acetogen isolated from livestock-impacted soil.
Phenotypic and phylogenetic studies were performed on three strains of an acetogenic bacterium isolated from livestock-impacted soil. The bacterium stained Gram-negative and was a non-spore-forming rod that was motile by peritrichous flagella. The novel strains had an optimum pH for growth of 8.0-8.5 and utilized H₂ : CO₂, CO : CO₂, glucose, fructose, mannose, turanose, ribose, trimethylamine, pyruvate, methanol, ethanol, n-propanol and n-butanol as growth substrates. Acetate was produced from glucose. Acetate, CO₂ and ethanol were produced from CO : CO₂. 16S rRNA gene sequence analysis indicated that the novel strains formed a new subline in the family Eubacteriaceae (rRNA cluster XV) of the low G+C-containing Gram-positive bacteria of the class Clostridia. The DNA G+C base composition was 34 mol%. Cell wall analysis revealed the existence of a novel B-type peptidoglycan similar to the B2α-type (B4) configuration with a variation containing aspartic acid. Based on phylogenetic and phenotypic evidence, it is proposed that the new isolates represent a novel genus and species, for which the name Alkalibaculum bacchi gen. nov., sp. nov. is proposed. The type strain of the type species is CP11(T) (=ATCC BAA-1772(T)=DSM 22112(T)).
Allen
Toby D
TD
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
Caldwell
Matthew E
ME
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
Lawson
Paul A
PA
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
Huhnke
Raymond L
RL
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Tanner
Ralph S
RS
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
D019342:Acetic Acid / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002248:Carbon Monoxide / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D058751:Livestock; D008124:Locomotion; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D001224:Aspartic Acid; D000431:Ethanol; D002248:Carbon Monoxide; D019342:Acetic Acid