title: Acafusin, a dimeric antifungal protein from Acacia confusa seeds.
abstract: A dimeric 34-kDa antifungal protein, designated as acafusin, was purified from Acacia confusa seeds using Q-Sepharose, DEAE-cellulose, Mono S and Superdex S75. It demonstrated antifungal activity toward Rhizoctonia solani with an IC(50) of 28 microM. Acafusin inhibited the activity of HIV-1 reverse transcriptase mildly with an IC(50) of 80 microM.
authors: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors: Sze Kwan
authors: SK
authors: Lam
authors: Tzi Bun
authors: TB
authors: Ng
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors; D012232:Rhizoctonia / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D002686:Chitin; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase