Production and characterization of poly-3-hydroxybutyrate from biodiesel-glycerol by Burkholderia cepacia ATCC 17759.
Glycerol, a byproduct of the biodiesel industry, can be used by bacteria as an inexpensive carbon source for the production of value-added biodegradable polyhydroxyalkanoates (PHAs). Burkholderia cepacia ATCC 17759 synthesized poly-3-hydroxybutyrate (PHB) from glycerol concentrations ranging from 3% to 9% (v/v). Increasing the glycerol concentration results in a gradual reduction of biomass, PHA yield, and molecular mass (M(n) and M(w)) of PHB. The molecular mass of PHB produced utilizing xylose as a carbon source is also decreased by the addition of glycerol as a secondary carbon source dependent on the time and concentration of the addition. (1)H-NMR revealed that molecular masses decreased due to the esterification of glycerol with PHB resulting in chain termination (end-capping). However, melting temperature and glass transition temperature of the end-capped polymers showed no significant difference when compared to the xylose-based PHB. The fermentation was successfully scaled up to 200 L for PHB production and the yield of dry biomass and PHB were 23.6 g/L and 7.4 g/L, respectively.
Zhu
Chengjun
C
Dept. of Environmental and Forest Biology, SUNY-College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
Nomura
Christopher T
CT
Perrotta
Joseph A
JA
Stipanovic
Arthur J
AJ
Nakas
James P
JP
D056804:Biofuels; D018533:Biomass; D016956:Burkholderia cepacia / Q000254:growth & development / Q000378:metabolism*; D004951:Esterification; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010443:Peptide Chain Termination, Translational; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D056804:Biofuels; D005990:Glycerol; D006885:Hydroxybutyrates; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates