[Isolation and determination of Neoechinulin A in Cordate Pinellia Tuber].
The Neoechinulin A in Cordate Pinellia Tuber was isolated by column chromatography and identified by mass spectrometry and nuclear magnetic resonance (NMR). A method of reversed-phase high performance liquid chromatography (RP-HPLC) for the determination of the Neoechinulin A in Cordate Pinellia Tuber was developed. The chromatography was performed on a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with a mixture of methanol and 0.1% phosphoric acid solution (63:37, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm and the column oven temperature was set at 30 degrees C. The volume of injection was 10 microL. There was a good linear relationship (r = 0.9995) between the mass concentration and the peak area of Neoechinulin A in the range of 2.0 - 40.0 mg/L. The recovery was 98.3% - 101.1%. The method is rapid and simple with good accuracy, reproducibility and suitable for the quality control of Cordate Pinellia Tuber from different sources.
Wang
Qi
Q
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Zhao
Yunli
Y
Gao
Xiaoxia
X
Wang
Xinyang
X
Yu
Zhiguo
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D026121:Indole Alkaloids / Q000032:analysis* / Q000302:isolation & purification*; D030021:Pinellia / Q000737:chemistry*; D010879:Piperazines / Q000032:analysis* / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids; D010879:Piperazines; C490266:neoechinulin A