[Effect of naphthalene and its derivatives on Candida pseudotropicalis 44 PK sensitivity to trichothecene mycotoxins].
Availability of T-2 toxin and HT-2 toxin in cereals and grain-crops is determined by a bioauthography or the method of paper disks using yeast Candida pseudotropicalis 44 pk. With the purpose of increasing the method sensitivity the culture medium for C.pseudotropicalis 44 pk was enriched by T-2 toxin, naphthalene, 1-naphthol, 2-naphthol, 1-naphthylacetate, 2-naphthylacetate, 1-naphthylamine and 1-nitroso-2-naphthol. The addition of any studied compounds, except for T-2 toxin, resulted in potentiation of toxic effect of T-2 toxin and HT-toxin. Introduction of 1-naphthylacetate in concentration of 0.16 mg/ml in the culture medium resulted in the 5-fold increase of sensitivity ofbioauthographic method to T-2 toxin and its 10-fold increase to HT-2 toxin. The modified bioauthographic method allows determining from 10 ng of T-2 toxin and from 100 ng of HT-2 toxin. Synergic action oftrichothecene mycotoxins and naphthalene derivatives enhances the risk of appearing of negative effects at simultaneous influence of these compounds on living organisms.
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D015374:Biosensing Techniques / Q000379:methods*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D004357:Drug Synergism; D002523:Edible Grain / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005670:Fusarium / Q000378:metabolism; D009281:Naphthalenes / Q000494:pharmacology*; D014255:Trichothecenes / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*
D009281:Naphthalenes; D014255:Trichothecenes; C031721:naphthalene