Photophysical properties of a new DyLight 594 dye.
We describe spectral properties of novel fluorescence probe DyLight 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.
Sarkar
Pabak
P
Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Sridharan
Savitha
S
Luchowski
Rafal
R
Desai
Surbhi
S
Dworecki
Boguslawa
B
Nlend
Marie
M
Gryczynski
Zygmunt
Z
Gryczynski
Ignacy
I
D000042:Absorption; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence
C417664:Alexa594; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D009930:Organic Chemicals