title: Morphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environments.
abstract: A polyphasic study of a benthic Nodularia isolate (LEGE06071) from an Atlantic environment, specifically salt pans, was performed. LEGE06071 resembled both type strains of Nodularia sphaerocarpa and Nodularia harveyana, while ACOI00729 (purchased isolate) was identified as N. sphaerocarpa. The length and width of vegetative cells varied from 3.10 to 3.15 microm and from 3.71 to 4.25 microm, respectively, while heterocyts were 3.91-4.89 microm long and 4.20-4.74 microm wide. None of the isolates had aerotopes. The sequencing of the 16S rRNA gene from the two Nodularia isolates indicated that they belonged neither to Nodularia spumigena nor N. harveyana. Nodularin and other cyanotoxin synthesis-associated genes could not be detected, nor could nodularin production be detected by ELISA. However, MALDI-TOF analysis of LEGE06071 revealed the presence of other compounds, namely, glycolipids. Hence, toxicological screenings against Artemia nauplii, Escherichia coli and Salmonella typhimurium were performed. Toxic effects could only be observed against Artemia, with 48 h-LC(50) values for the aqueous and crude extract of methanol of 53.21 mg ml(-1) and 17.81 mg ml(-1), respectively. This study presents the first evidence of a non-nodularin-producing Nodularia isolate in Atlantic salt pan ecosystems and its potential ecotoxicity against Artemia sp.
authors: CIIMAR/CIMAR-LA, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal. vlopes@ciimar.up.pt
authors: Viviana R
authors: VR
authors: Lopes
authors: Agostinho
authors: A
authors: Antunes
authors: Martin
authors: M
authors: Welker
authors: Rosário F
authors: RF
authors: Martins
authors: Vítor M
authors: VM
authors: Vasconcelos
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects / Q000254:growth & development; D001267:Atlantic Ocean; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis / Q000633:toxicity; D008969:Molecular Sequence Data; D046932:Nodularia / Q000145:classification / Q000166:cytology* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S