Oogenesis and programmed cell death of nurse cells in the endoparasitoid, Pteromalus puparum.
In this study, we describe the features of oogenesis in the endoparasitoid, Pteromalus puparum, as well as the distinct type of programmed cell death of the nurse cells through conventional light and fluorescent markers for apoptosis and immunofluorescent analysis. Oogenesis in this endoparasitoid is divided into five stages, of which stages 1-2, 3-4, and 5 are corresponding to previtellogenic growth, yolk uptake, and the formation of egg envelopes, respectively. From these studies, we demonstrate two critical events, which are vitellogenin absorption and rapid transfer of nurse cell content, resulting in remarkable increase in the volume of oocytes during oogenesis in this endoparasitoid. Vitellogenin absorption initiates in the oocyte of early stage 3, and bulk transfer of nurse cell content into the oocyte occurs at stage 4 of oogenesis in P. puparum, which is mainly characterized with the programmed cell death in the nurse cell complex.
Dong
Sheng-Zhang
SZ
State Key Laboratory of Rice Biology and Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Ye
Gong-Yin
GY
Guo
Jian-Yang
JY
Yu
Xiao-Ping
XP
Hu
Cui
C
D000818:Animals; D017209:Apoptosis; D005260:Female; D006927:Hymenoptera / Q000166:cytology / Q000502:physiology*; D008853:Microscopy; D008856:Microscopy, Fluorescence; D009866:Oogenesis