GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.
The lacto-series gangliosides 3'-isoLM1 and 3',6'-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3'-isoLM1 and 3',6'-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene beta3Gn-T5 knockout mice. In this study, we immunized beta3Gn-T5 knockout mice with 3'-isoLM1/3',6'-isoLD1 and produced the anti-3'-isoLM1/3',6'-isoLD1 mAb GMab-1, of the IgG(3) subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.
Kato
Yukinari
Y
Department of Pathology, Duke University Medical Center, DUMC-3156, Durham, NC 27710, USA.
Kuan
Chien-Tsun
CT
Chang
Jinli
J
Kaneko
Mika Kato
MK
Ayriss
Joanne
J
Piao
Hailan
H
Chandramohan
Vidyalakshmi
V
Pegram
Charles
C
McLendon
Roger E
RE
Fredman
Pam
P
Månsson
Jan-Eric
JE
Bigner
Darell D
DD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D005910:Glioma / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005732:Gangliosides; D007074:Immunoglobulin G; C078813:IV(3)-NeuAc-LcOse4Cer; C078812:IV(3)-NeuAc-III(6)-NeuAc-LcOse4Cer; D017351:N-Acetylglucosaminyltransferases; C067641:lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase