Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf and MAPK signal transduction pathway on inducible nitric oxide synthase expression in alveolar macrophage of chronic bronchitis rats.
The goal of this study was to investigate the possible therapy mechanism of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. Leaf (TAL) in alveolar macrophage (AM) of chronic bronchitis (CB) rats. CB model was established by injection of bacillus calmette guein (BCG) plus lipopolisacharide (LPS) in rats. TAL significantly inhibited the increased NO concentration, iNOS expression and phosphorylation of p38 MAPK in alveolar macrophages (AMs) of CB rats. Using in vivo test, we found that SB203580, a p38 MAPK inhibitor, (10 muM) significantly inhibited inducible nitric oxide synthase (iNOS) mRNA expression in AM. This data indicate that TAL highly decreases excessive iNOS expression and NO induction, and p38 MAPK signal transduction participates in iNOS expression and NO induction in AM of CB rats. The effect of TAL on iNOS expression in AM may be related to its inhibition of p38 MAPK signal transduction.
Huang
Y
Y
School of Pharmacy, Anhui Medical University, Hefei 230032, Anhui Province, China.
Li
J
J
Meng
X M
XM
Jiang
G L
GL
Li
H
H
Cao
Q
Q
Yu
S C
SC
Lv
X W
XW
Cheng
W M
WM
D000818:Animals; D029481:Bronchitis, Chronic / Q000188:drug therapy / Q000378:metabolism* / Q000382:microbiology; D004791:Enzyme Inhibitors; D031983:Eriobotrya / Q000737:chemistry*; D007093:Imidazoles; D008070:Lipopolysaccharides; D016676:Macrophages, Alveolar / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D009163:Mycobacterium bovis; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D011725:Pyridines; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D007093:Imidazoles; D008070:Lipopolysaccharides; D010936:Plant Extracts; D011725:Pyridines; D012333:RNA, Messenger; D014315:Triterpenes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580