title: Neuronal subtype specification within a lineage by opposing temporal feed-forward loops.
abstract: Neural progenitors generate distinct cell types at different stages, but the mechanisms controlling these temporal transitions are poorly understood. In the Drosophila CNS, a cascade of transcription factors, the "temporal gene cascade," has been identified that acts to alter progenitor competence over time. However, many CNS lineages display broad temporal windows, and it is unclear how broad windows progress into subwindows that generate unique cell types. We have addressed this issue in an identifiable Drosophila CNS lineage and find that a broad castor temporal window is subdivided by two different feed-forward loops, both of which are triggered by castor itself. The first loop acts to specify a unique cell fate, whereas the second loop suppresses the first loop, thereby allowing for the generation of alternate cell fates. This mechanism of temporal and "subtemporal" genes acting in opposing feed-forward loops may be used by many stem cell lineages to generate diversity.
authors: Department of Clinical and Experimental Medicine, Linkoping University, Linkoping SE-581 85, Sweden.
authors: Magnus
authors: M
authors: Baumgardt
authors: Daniel
authors: D
authors: Karlsson
authors: Javier
authors: J
authors: Terriente
authors: Fernando J
authors: FJ
authors: Díaz-Benjumea
authors: Stefan
authors: S
authors: Thor
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C490626:NAB protein, Drosophila; D012097:Repressor Proteins; C521136:Sqz protein, Drosophila; D014157:Transcription Factors; C110593:cas protein, Drosophila; C062330:grh protein, Drosophila; C104357:kn protein, Drosophila