Evaluation of a single sampling method for estimation of plasma iohexol clearance in dogs and cats with various kidney functions.
Plasma iohexol clearance (PCio) is a practical method for measuring the glomerular filtration rate (GFR) in clinical settings. However, it is too time-consuming for routine application and requires hospitalization for at least half a day. Therefore, the development of a simpler procedure for plasma clearance is necessary to allow the frequent measurement of GFR in clinical settings. The purpose of the present study was to evaluate a single sampling method for estimation of PCio in dogs and cats with various kidney functions. The PCio determined by the 1-compartment model using 3 samples (PCio (3samples)) was used as a reference for the evaluation of the single sampling method (PCio (single)). Plasma iohexol concentration was determined by a cerium arsenite colorimetric method. PCio single was calculated using the equation obtained by nonlinear regression analysis. PCio (single) was significantly correlated with PCio (3samples) in both dogs and cats (dogs: R(2)=0.985, P<0.001, cats: R(2)=0.986, P<0.001). In a receiver operating characteristics analysis, the area under the curve, sensitivity, and specificity for detecting decreased GFR were 0.995 [SE, 0.003], 98%, and 93% for dogs and 0.993 [SE, 0.003], 98%, and 93% for cats, respectively. These results demonstrate that PCio (single) may be very useful for the detection of decreasing GFR in dogs and cats.
Miyagawa
Yuichi
Y
Laboratory of Veterinary Integrative Medicine, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan Cho, Musashino City, Tokyo, 180-8602, Japan. d0607@nvlu.ac.jp
Takemura
Naoyuki
N
Hirose
Hisashi
H
D000818:Animals; D000830:Animals, Laboratory; D015415:Biomarkers / Q000097:blood*; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis; D002415:Cats; D003124:Colorimetry; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis; D004285:Dogs; D005919:Glomerular Filtration Rate / Q000502:physiology*; D007472:Iohexol / Q000378:metabolism*; D007674:Kidney Diseases / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007677:Kidney Function Tests; D008657:Metabolic Clearance Rate; D012016:Reference Values; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D015415:Biomarkers; D007472:Iohexol