CE-MALDI interface based on inkjet technology.
An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by the separation of a set of standard peptides. That separation generated up to 40 000 theoretical plates in less than 3 min. Detection limits were 500 amol for an ABI TOF-TOF instrument and 2 fmol for an ABI Q-TOF instrument. Over 70% coverage was obtained for the tryptic digest of alpha-lactalbumin in less than 2.5 min.
Vannatta
Michael W
MW
Department of Chemistry, University of Washington, Seattle, WA, USA.
Whitmore
Colin D
CD
Dovichi
Norman J
NJ
D000804:Angiotensin II / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*
D010455:Peptides; D000804:Angiotensin II