A high-throughput method for determining chloramphenicol residues in poultry, egg, shrimp, fish, swine and bovine using LC-ESI-MS/MS.
A LC-ESI-MS/MS method for determining chloramphenicol residues in fish, shrimp, poultry, eggs, bovine and swine was developed and validated. The samples were extracted with a phosphate extraction solution followed by liquid-liquid extraction with ethyl acetate. The LC was performed on a C18 column at room temperature. For all the matrices, the analytical curves showed r values and recoveries higher than 0.99 and 50%, respectively. The accuracy values lay between 85 and 120% and the precision was lower than 20%. The LOQ was 0.1 ng/g. The method was employed to analyze samples collected in Brazil.
Rocha Siqueira
Silvia Regina
SR
Galeno Research Unit, Parque Taquaral, Campinas, SP, Brazil.
Luiz Donato
José
J
de Nucci
Gilberto
G
Reyes
Felix Guillermo Reyes
FG
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D001938:Brazil; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D033561:Penaeidae / Q000737:chemistry; D011200:Poultry; D012758:Shellfish / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol