[On the modified process of human hemoglobin based blood substitutes].
Purified hemoglobin was modified with pyridoxal 5-phosphate(PLP) and polymerized with glutaric dialdehyde(GDA) to get the products. By comparison of the physical, chemical and biological properties of different procedures for modification before and after polymerization, there is no significant difference in molecular distribution, methemoglobin(MetHb) concentration, oxygen carrier capacity, P50 and spectra. Furthermore, the procedure of modification after polymerization can save PLP greatly and decrease cost greatly. So the procedure of modification after polymerization is a better way in research and production. The addition of GDA could control the increasing of MetHb. By comparison on the physical, chemical and biological properties of different procedures, there is no significant difference in molecular distribution, MetHb concentration, oxygen carrier capacity and spectra between the procedure of adding GDA before PLP and that after PLP. But the P50 of adding GDA before PLP is much lower than that after PLP. So the procedure of adding GDA after PLP is a better way.
Li
Fengjuan
F
College of Science and Technology, Tianjin University, Tianjin 300071, China. lifengjuan12@163.com
Zhang
Honghui
H
Wang
Jinfeng
J
Yang
Chengmin
C
D001802:Blood Substitutes; D055598:Chemical Phenomena; D005976:Glutaral / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008706:Methemoglobin / Q000737:chemistry; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000737:chemistry
D001802:Blood Substitutes; D006454:Hemoglobins; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate; D008706:Methemoglobin; D005976:Glutaral