title: Immunohistochemical localization and gene expression of carbonic anhydrase isoenzymes CA-II and CA-VI in canine lower airways and lung.
abstract: The immunohistolocalization and gene expression of carbonic anhydrase (CA) isoenzymes CA-II and CA-VI in the canine lower airways and lung were examined using specific canine CA-II and CA-VI antisera and the RT-PCR method. Laryngeal, tracheal and bronchial epithelia, serous acinar and bronchiolar secretory cells and pulmonary great alveolar cells showed immunopositive reactions to anti-CA-II and anti-CA-VI antisera. However, all mucous cells showed immunonegative reactions. The physiological roles of CA-II and CA-VI in the lower airways and lung may involve the maintenance of pH balance and the protection of mucosal surfaces against the acidic milieu.
authors: Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
authors: Yosuke
authors: Y
authors: SUGIURA
authors: Motoharu
authors: M
authors: OISHI
authors: Tomoko
authors: T
authors: AMASAKI
authors: Satoshi
authors: S
authors: SOETA
authors: Nobutsune
authors: N
authors: ICHIHARA
authors: Toshiho
authors: T
authors: NISHITA
authors: Masaru
authors: M
authors: MURAKAMI
authors: Hajime
authors: H
authors: AMASAKI
authors: Masao
authors: M
authors: ASARI
mesh_terms: D000818:Animals; D024402:Carbonic Anhydrase II / Q000235:genetics / Q000378:metabolism*; D030741:Carbonic Anhydrase IV / Q000235:genetics / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007527:Isoenzymes; D008168:Lung / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D024402:Carbonic Anhydrase II; D030741:Carbonic Anhydrase IV