Diverse pathogenicity of equine herpesvirus 1 (EHV-1) isolates in CBA mouse model.
The pathogenicity of equine herpesvirus 1 (EHV-1) isolates of Japan were evaluated by using the CBA mouse model. CBA mice were inoculated with eight Japanese EHV-1 strains (89c1, 90c16, 90c18, 97c11, 98c12, 00c19, 01c1 and HH-1) and one British strain (Ab4p). 89c1 caused slight body weight loss and nervous signs in mice at 8 days post infection (dpi). Severe weight loss and nervous signs were observed in mice inoculated with Ab4p at 6 dpi. The other strains did not cause apparent clinical signs. Infectious viruses were recovered from the lungs of all groups at 2 dpi. Histopathological analysis revealed interstitial pneumonia in the lungs of all mice inoculated with EHV-1. Encephalitis or meningoencephalitis was observed in the brains of mice inoculated with 89c1, 90c18, 97c11, 98c12, 01c1 and Ab4p. Japanese EHV-1 strains showed low pathogenicity in CBA mice, whereas the sequential affects of infection are similar to those of the highly pathogenic strain Ab4p. These results suggest that field isolates of EHV-1 have varying degrees of pathogenicity in CBA mice.
Yu
Mi Htay Htay
MH
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Kasem
Samy Gomaa Ahmed
SG
Tsujimura
Koji
K
Matsumura
Tomio
T
Yanai
Tokuma
T
Yamaguchi
Tsuyoshi
T
Ohya
Kenji
K
Fukushi
Hideto
H
D000818:Animals; D001835:Body Weight; D001921:Brain / Q000473:pathology / Q000821:virology; D006566:Herpesviridae Infections / Q000473:pathology*; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification / Q000472:pathogenicity*; D006734:Horse Diseases / Q000473:pathology / Q000821:virology; D006736:Horses; D007564:Japan; D008587:Meningitis, Viral / Q000473:pathology / Q000662:veterinary / Q000821:virology; D051379:Mice; D008808:Mice, Inbred CBA; D009422:Nervous System Diseases / Q000473:pathology / Q000662:veterinary / Q000821:virology; D012141:Respiratory Tract Infections / Q000473:pathology / Q000662:veterinary / Q000821:virology; D013045:Species Specificity