Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha.
Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1alpha (eEF1alpha) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1alpha and generated a ZPR1 mutant that constitutively interacted with eEF1alpha. ZPR1-DeltaA (Delta193-246) bound to eEF1alpha independently of Zn(2+) in vivo. This study indicates that ZPR1-DeltaA (Delta193-246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1alpha.
Yanaka
Noriyuki
N
Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. yanaka@hiroshima-u.ac.jp
Kaseda
Yurika
Y
Tanaka
Anna
A
Nogusa
Yoshihito
Y
Sumiyoshi
Naoki
N
Kato
Norihisa
N
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011485:Protein Binding
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D050505:Mutant Proteins; D020648:Peptide Elongation Factor 1; C490987:Zfp259 protein, mouse