Tula hantavirus NSs protein accumulates in the perinuclear area in infected and transfected cells.
The small RNA segment of some hantaviruses (family Bunyaviridae) encodes two proteins: the nucleocapsid protein and, in an overlapping reading frame, a non-structural (NSs) protein. The hantavirus NSs protein, like those of orthobunya- and phleboviruses, counteracts host innate immunity. Here, for the first time, the NSs protein of a hantavirus (Tula virus) has been observed in infected cells and shown to localize in the perinuclear area. Transiently expressed NSs protein showed similar localization, although the kinetics was slightly different, suggesting that to reach its proper location in the infected cell, the NSs protein does not have to cooperate with other viral proteins.
Virtanen
Jussi Oskari
JO
Infection Biology Research Program, Department of Virology, Haartman Institute, University of Helsinki, PO Box 21, Haartmaninkatu 3, 00014 Helsinki, Finland. oskari.virtanen@helsinki.fi
Jääskeläinen
Kirsi Maria
KM
Djupsjöbacka
Janica
J
Vaheri
Antti
A
Plyusnin
Alexander
A
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006476:Orthohantavirus / Q000235:genetics / Q000378:metabolism*; D018778:Hantavirus Infections / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D021381:Protein Transport; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
D017361:Viral Nonstructural Proteins