Decreased serum prolidase activity and increased oxidative stress in early pregnancy loss.
OBJECTIVE
To compare the levels of serum prolidase activity and oxidative stress markers including total oxidative status (TOS), total antioxidant capacity (TAC), lipid hydroperoxide (LOOH) and total free sulfhydryl (-SH) in healthy pregnant women without early pregnancy loss (EPL) and women with EPL.


METHODS
Serum samples were obtained from 45 healthy first-trimester pregnancies and 45 pregnancies complicated with EPL. We have measured serum prolidase activity, TAC, TOS, -SH and LOOH levels spectrophotometrically.


RESULTS
Serum prolidase, TAC and -SH levels were significantly lower in the women with EPL than in the women without EPL (p <0.001, p = 0.001, p < 0.001, respectively), whereas TOS and LOOH levels were significantly higher (p < 0.001, p = 0.004, respectively). Prolidase activity was negatively correlated with TOS and LOOH levels (r = -0.299, p = 0.004; r = -0.323, p = 0.002, respectively), while positively correlated with TAC and -SH levels (r = 0.232, p = 0.028, and r = 0.418, p <0.001, respectively)


CONCLUSIONS
Findings of this study have shown that serum prolidase activity and oxidative stress are significantly associated with the presence of EPL, and the correlation between serum prolidase activity and the markers of oxidative stress was reflected in increased serum TOS and LOOH levels and decreased serum TAC and -SH levels.
Toy
Harun
H
Department of Obstetrics and Gynecology, School of Medicine, Harran University, Yenisehir Kampusu No: 23/2, Sanliurfa, Turkey. haruntoy@hotmail.com
Camuzcuoglu
Hakan
H
Camuzcuoglu
Aysun
A
Celik
Hakim
H
Aksoy
Nurten
N
D000022:Abortion, Spontaneous / Q000097:blood / Q000201:enzymology*; D000328:Adult; D000975:Antioxidants / Q000032:analysis; D004150:Dipeptidases / Q000097:blood*; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D018709:Statistics, Nonparametric; D013438:Sulfhydryl Compounds / Q000097:blood
D000975:Antioxidants; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D004150:Dipeptidases; C022449:proline dipeptidase