Microfluidic in vivo screen identifies compounds enhancing neuronal regeneration.
Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens for compounds enhancing neuronal regeneration. These screens are enabled by the microfluidic devices we have developed for C. elegans. The devices enable rapid and repeatable animal immobilization which allows high-throughput and precise surgery. Following surgery, animals are exposed to the contents of a small-molecule library and assayed for neuronal regeneration. Using this screening method we have identified several compounds that enhance neural regeneration in vivo.
Rohde
Christopher B
CB
Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Gilleland
Cody
C
Samara
Chrysanthi
C
Norton
Stephanie
S
Haggarty
Stephen
S
Yanik
Mehmet Fatih
MF
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage*
D018696:Neuroprotective Agents