[Production and identification of monoclonal antibody against human S100A4.].
OBJECTIVE
To prepare specific monoclonal antibody against human S100A4 protein and establish a reliable method to detect S100A4 protein in tumor specimens.


METHODS
A standard hybridoma method was used to generate monoclonoal antibodies against recombinant human S100A4. ELISA, Western blot and immunohistochemistry were used to validate this antibody and the antibody is used to examine specimens of human breast and colon cancers.


RESULTS
One hybridoma cell line which secreted monoclonal antibody specifically against recombinant human S100A4 protein was obtained and named as D101. The monoclonal antibody is shown to be more specific for S100A4 protein than the polyclonal antibody, at least in Western blot. The monoclonal antibody is suitable for detecting the expression of S100A4 in specimens from human tissues and gave results consistent with those of a commercially available polyclonal antibody in a small group of breast and colorectal carcinomas.


CONCLUSIONS
The monoclonal antibody is specific for S100A4 and can be produced on a large scale; therefore it will be more reproducible for future clinical applications.
Hu
Xiang-Yang
XY
School of Clinical Medicine, Southeast University, Nanjing 210009, China.
Huang
Xiao-Ming
XM
Zhang
Nan
N
He
Dao-Wei
DW
Ye
Yin-Ying
YY
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D007150:Immunohistochemistry
D000911:Antibodies, Monoclonal