The implication of YggT of Escherichia coli in osmotic regulation.
An Escherichia coli mutant lacking three major K(+) uptake systems, Trk, Kup, and Kdp, did not grow under low K(+)and high Na(+) concentrations. The introduction of fkuA and of fkuB of a marine bacterium, Vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of K(+) uptake (Nakamura, Katoh, Shimizu, Matsuba, and Unemoto, Biochim. Biophys. Acta, 1277, 201-208 (1996)). We investigated the function of unknown genes of E. coli, yggS and yggT, homologs of fkuA and fkuB respectively. E. coli TK2420 cells, which lack the three K(+) uptake systems, did not grow under high Na(+) or mannitol concentrations. The growth defect was compensated by the introduction of the yggT gene alone: yggS was not required. Here we found that YggT endowed E. coli cells with a tolerance for osmotic shock, and discuss a possible mechanism.
Ito
Tomokazu
T
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan.
Uozumi
Nobuyuki
N
Nakamura
Tatsunosuke
T
Takayama
Sayuri
S
Matsuda
Nobuyuki
N
Aiba
Hirofumi
H
Hemmi
Hisashi
H
Yoshimura
Tohru
T
D000596:Amino Acids / Q000378:metabolism; D049109:Cell Proliferation; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017136:Ion Transport; D009154:Mutation; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism
D000596:Amino Acids; D029968:Escherichia coli Proteins; C547503:YggT protein, E coli; D011188:Potassium