The inhibition of advanced glycation end-products-induced retinal vascular permeability by silver nanoparticles.
The increased permeability of the blood-retinal barrier is known to occur in patients with diabetes, and this defect contributes to retinal edema. This study aimed to determine the effects of silver nanoparticles (Ag-NPs) on advanced glycation end-products (AGEs)-induced endothelial cell permeability. Cultured porcine retinal endothelial cells (PRECs) were exposed to AGE-modified bovine serum albumin (AGE-BSA) and the endothelial cell permeability was detected by measuring the flux of RITC-dextran across the PREC monolayers. We found that AGE-BSA increased the dextran flux across a PREC monolayer and Ag-NPs blocked the solute flux induced by AGE-BSA. In order to understand the underlying signaling mechanism of Ag-NPs on the inhibitory effect of AGE-BSA-induced permeability, we demonstrated that Ag-NPs could inhibit the AGE-BSA-induced permeability via Src kinase pathway. AGE-BSA also increased the PREC permeability by stimulating the expression of intracellular adhesion molecule-1 (ICAM-1) and decreased the expression of occludin and ZO-1. Further, Ag-NPs inhibited the AGE-BSA-induced permeability by increased expression of tight junction proteins occludin and ZO-1, co-incident with an increase in barrier properties of endothelial monolayer. Together, our results indicate that Ag-NPs could possibly act as potent anti-permeability molecule by targeting the Src signaling pathway and tight junction proteins and it offers potential targets to inhibit the ocular related diseases.
Sheikpranbabu
Sardarpasha
S
Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil-626190, Tamilnadu, India.
Kalishwaralal
Kalimuthu
K
Lee
Kyung-Jin
KJ
Vaidyanathan
Ramanathan
R
Eom
Soo Hyun
SH
Gurunathan
Sangiliyandi
S
D000818:Animals; D001813:Blood-Retinal Barrier / Q000378:metabolism*; D002199:Capillary Permeability / Q000502:physiology*; D002417:Cattle; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D012171:Retinal Vessels / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D012834:Silver / Q000378:metabolism*; D013552:Swine; D019108:Tight Junctions / Q000378:metabolism; D019061:src-Family Kinases / Q000378:metabolism
D017127:Glycation End Products, Advanced; C081628:advanced glycation end products-bovine serum albumin; D018799:Intercellular Adhesion Molecule-1; D012710:Serum Albumin, Bovine; D012834:Silver; D019061:src-Family Kinases