Bench to batch: advances in plant cell culture for producing useful products.
Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.
Weathers
Pamela J
PJ
Department of Biology and Biotechnology at Gateway, Worcester Polytechnic Institute, Worcester, MA 01609, USA. weathers@wpi.edu
Towler
Melissa J
MJ
Xu
Jianfeng
J
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D018506:Gene Expression Regulation, Plant; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis*
D003470:Culture Media; D010940:Plant Proteins; D011994:Recombinant Proteins