Rapid screening for genotypes as possible markers of virulence in the neurotropic black yeast Exophiala dermatitidis using PCR-RFLP.
A simple method for fungal genotype screening was developed for the black yeast Exophiala dermatitidis based on RFLP of ribosomal ITS regions currently used as potential virulence markers. In a study set of 502 strains of the species, two main genotypes were recognized. Only 0.97% of lanes were difficult to interpret as they did not clearly present one of the expected genotypes. Twenty strains were deviating and proved to be E. spinifera after sequencing. Eight common, related species (based on SSU data) with clinical significance yielded different patterns with TaqI digestion, and thus the method is also usable for routine diagnostics.
Sudhadham
M
M
Centraalbureau voor Schimmelcultures Fungal Biodiversity Centre, Utrecht, The Netherlands.
de Hoog
G S
GS
Menken
S B J
SB
Gerrits van den Ende
A H G
AH
Sihanonth
P
P
D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005093:Exophiala / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D005838:Genotype; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D014774:Virulence
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer