title: Purification and characterization of an organic solvent-tolerant lipase from Pseudomonas aeruginosa CS-2.
abstract: An extracellular lipase secreted by Pseudomonas aeruginosa CS-2 was purified to homogeneity about 25.5-fold with an overall yield of 45.5%. The molecular mass of the lipase was estimated to be 33.9 kDa by SDS-PAGE and 36 kDa by gel filtration. The optimum temperature and pH were 50 degrees C and 8.0. The lipase was found to be stable at pH 4-10 and below 50 degrees C. Its hydrolytic activity was highest against p-nitrophenyl palmitate (p-NPP) among p-nitrophenyl esters of fatty acids with various chain lengths. The lipase was activated in the presence of Ca(2+), while it was inactivated by other metal ions more or less. EDTA significantly reduced the lipase activity, indicating the lipase was a metalloenzyme. Gum Arabic and polyvinyl alcohol 124 enhanced lipase activity but Tween-20, Tween-80, and hexadecyltrimethyl ammonium bromide strongly inhibited the lipase. It exhibited stability in some organic solvents. The lipase was activated in the presence of acetonitrile. Conversely, it was drastically inactivated by methanol and ethanol.
authors: New World Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. renpeng23@hotmail.com.
authors: Ren
authors: R
authors: Peng
authors: Jinping
authors: J
authors: Lin
authors: Dongzhi
authors: D
authors: Wei
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012997:Solvents; D008049:Lipase