Protective effect of supplemental anthocyanins on Arabidopsis leaves under high light.
Ten anthocyanin components have been detected in roots of purple sweet potato (Ipomoea batatas Lam.) by high-performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. All the anthocyanins were exclusively cyanidins or peonidin 3-sophoroside-5-glucosides and their acylated derivatives. The total anthocyanin content in purple sweet potato powder obtained by solid-phase extraction was 66 mg g(-1). A strong capacity of purple sweet potato anthocyanins (PSPA) to scavenge reactive oxygen species (superoxide, hydroxyl radical) and the stable 1,1-diphenyl-2-picrylhydrazyl organic free radical was found in vitro using the electron spin resonance technique. To determine the functional roles of anthocyanins in leaves in vivo, for the first time, supplemental anthocyanins were infiltrated into leaves of Arabidopsis thaliana double mutant of the ecotype Landsberg erecta (tt3tt4) deficient in anthocyanin biosynthesis. Chlorophyll fluorescence imaging showed that anthocyanins significantly ameliorated the inactivation of photosystems II during prolonged high-light (1300 micromol m(-2) s(-1)) exposure. Comet assay of DNA revealed an obvious role of supplemental PSPA in alleviating DNA damage by high light in leaves. Our results suggest that anthocyanins could function in vitro and in vivo to alleviate the direct or indirect oxidative damage of the photosynthetic apparatus and DNA in plants caused by high-light stress.
Zeng
Xue-Qin
XQ
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, South China Normal University, Guangzhou 510631, Guangdong, China.
Chow
Wah Soon
WS
Su
Li-Juan
LJ
Peng
Xin-Xiang
XX
Peng
Chang-Lian
CL
D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification; D017360:Arabidopsis / Q000378:metabolism / Q000528:radiation effects*; D002734:Chlorophyll / Q000378:metabolism; D004249:DNA Damage; D018744:DNA, Plant / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000737:chemistry; D027723:Ipomoea batatas / Q000737:chemistry; D008027:Light; D018384:Oxidative Stress; D045332:Photosystem II Protein Complex / Q000528:radiation effects; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects*
D000872:Anthocyanins; D018744:DNA, Plant; D016166:Free Radical Scavengers; D045332:Photosystem II Protein Complex; D002734:Chlorophyll