title: Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques.
abstract: Vitamin B(12) was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B(12)/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B(12) for analysis. The presence of the K(+) adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization-mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.
authors: Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 020, India.
authors: Arenahalli Ningegowda
authors: AN
authors: Madhu
authors: Prashant
authors: P
authors: Giribhattanavar
authors: Mandayam Singara
authors: MS
authors: Narayan
authors: Siddalingaiya Gurudutt
authors: SG
authors: Prapulla
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002712:Chlorides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D019936:Probiotics / Q000378:metabolism*; D013997:Time Factors; D014805:Vitamin B 12 / Q000096:biosynthesis*; D017967:Zinc Compounds / Q000494:pharmacology
chemical_list: D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D014805:Vitamin B 12