title: Aspergillus fumigatus conidial melanin modulates host cytokine response.
abstract: Melanin biopigments have been linked to fungal virulence. Aspergillus fumigatus conidia are melanised and are weakly immunogenic. We show that melanin pigments on the surface of resting Aspergillus fumigatus conidia may serve to mask pathogen-associated molecular patterns (PAMPs)-induced cytokine response. The albino conidia induced significantly more proinflammatory cytokines in human peripheral blood mononuclear cells (PBMC), as compared to melanised wild-type conidia. Blocking dectin-1 receptor, Toll-like receptor 4 or mannose receptor decreased cytokine production induced by the albino but not by the wild type conidia. Moreover, albino conidia stimulated less potently, cytokine production in PBMC isolated from an individual with defective dectin-1, compared to the stimulation of cells isolated from healthy donors. These results suggest that β-glucans, but also other stimulatory PAMPs like mannan derivatives, are exposed on conidial surface in the absence of melanin. Melanin may play a modulatory role by impeding the capability of host immune cells to respond to specific ligands on A. fumigatus.
authors: Department of Medicine, Radboud University Nijmegen Medical Centre, P.O. Box 9101, Geert Grootplein 8, 6525 GA, Nijmegen, The Netherlands.
authors: Louis Y A
authors: LY
authors: Chai
authors: Mihai G
authors: MG
authors: Netea
authors: Janyce
authors: J
authors: Sugui
authors: Alieke G
authors: AG
authors: Vonk
authors: Wendy W J
authors: WW
authors: van de Sande
authors: Adilia
authors: A
authors: Warris
authors: Kyung J
authors: KJ
authors: Kwon-Chung
authors: Bart Jan
authors: BJ
authors: Kullberg
mesh_terms: D001228:Aspergillosis / Q000276:immunology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology* / Q000472:pathogenicity*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D054884:Host-Pathogen Interactions; D006801:Humans; D007963:Leukocytes, Mononuclear; D008543:Melanins / Q000276:immunology*; D013172:Spores, Fungal / Q000276:immunology*; D014774:Virulence
chemical_list: D016207:Cytokines; D008543:Melanins