title: Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements.
abstract: We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.
authors: The Group of Biomedical Image Technologies, Universidad Politcnica de Madrid, Madrid 28040 Spain. david.pastor@die.upm.es
authors: D
authors: D
authors: Pastor
authors: M A
authors: MA
authors: Luengo-Oroz
authors: B
authors: B
authors: Lombardot
authors: I
authors: I
authors: Gonzalvez
authors: L
authors: L
authors: Duloquin
authors: T
authors: T
authors: Savy
authors: P
authors: P
authors: Bourgine
authors: N
authors: N
authors: Peyrieras
authors: A
authors: A
authors: Santos
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D047108:Embryonic Development / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology* / Q000502:physiology