title: Protein identification using Sorcerer 2 and SEQUEST.
abstract: Sage-N's Sorcerer 2 provides an integrated data analysis system for comprehensive protein identification and characterization. It runs on a proprietary version of SEQUEST(R), the most widely used search engine for identifying proteins in complex mixtures. The protocol presented here describes the basic steps performed to process mass spectrometric data with Sorcerer 2 and how to analyze results using TPP and Scaffold. The unit also provides an overview of the SEQUEST(R) algorithm, along with Sorcerer-SEQUEST(R) enhancements, and a discussion of data filtering methods, important considerations in data interpretation, and additional resources that can be of assistance to users running Sorcerer and interpreting SEQUEST(R) results.
authors: Department of Cell Biology, Center for Vascular Biology, University of Connecticut Health Center, Farmington, Connecticut, USA.
authors: Deborah H
authors: DH
authors: Lundgren
authors: Harryl
authors: H
authors: Martinez
authors: Michael E
authors: ME
authors: Wright
authors: David K
authors: DK
authors: Han
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software; D012985:Software Design
chemical_list: D011506:Proteins