Comparison of three alternative methods for analysis of equine Faecal Egg Count Reduction Test data.
The Faecal Egg Count Reduction Test (FECRT) is the most widely used method of assessing the efficacy of anthelmintics, and is the only in vivo technique currently approved for use with horses. Equine Faecal Egg Count (FEC) data are frequently characterised by a low mean, high variability, small sample size and frequent zero count observations. Accurate analysis of the data therefore depends on the use of an appropriate statistical technique. Analyses of simulated FECRT data by methods based on calculation of the empirical mean and variance, non-parametric bootstrapping, and Markov chain Monte Carlo (MCMC) are compared. The MCMC method consistently outperformed the other methods, independently of the distribution from which the data were generated. Bootstrapping produced notional 95% confidence intervals containing the true parameter as little as 40% of the time with sample sizes of less than 50. Analysis of equine FECRT data yielded inconclusive results in 53 of 63 (84%) datasets, suggesting that the routine use of prior sample size calculations should be adopted to ensure sufficient data are collected. The authors conclude that computationally intensive parametric methods such as MCMC be used for analysis of FECRT data with sample sizes of less than 50, in order to avoid erroneous inference about the true efficacy of anthelmintics in the field.
Denwood
M J
MJ
Boyd Orr Centre for Population and Ecosystem Health, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Glasgow, UK. m.denwood@vet.gla.ac.uk
Reid
S W J
SW
Love
S
S
Nielsen
M K
MK
Matthews
L
L
McKendrick
I J
IJ
Innocent
G T
GT
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D005243:Feces / Q000469:parasitology*; D006374:Helminthiasis, Animal / Q000188:drug therapy* / Q000453:epidemiology; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D009426:Netherlands / Q000453:epidemiology; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary*
D000871:Anthelmintics