Serum biomarkers of renal cell carcinoma assessed using a protein profiling approach based on ClinProt technique.
OBJECTIVE
To investigate the possibility of using the ClinProt technique to find serum cancer related diagnostic markers that are able to better discriminate healthy subjects from patients affected by renal cell carcinoma (ccRCC). Renal cell carcinoma is the most common malignancy of the kidney. Biomarkers for early detection, prognosis, follow-up, and differential diagnosis of ccRCC from benign renal lesions are needed in daily clinical practice when imaging is not helpful.


METHODS
Serum of 29 healthy subjects and 33 ccRCC patients was analyzed by the ClinProt/MALDI-ToF technique.


RESULTS
A cluster of 3 peptides (A = m/z 1083 +/- 8 Da, B = m/z 1445 +/- 8 Da and C = m/z 6879 +/- 8 Da) was able to discriminate patients from control subjects. Cross-validation analysis using the whole casistic showed 88% and 96% of sensitivity and specificity, respectively. Moreover, the cluster showed 100% sensitivity for the identification of patients at pT2 (n = 5) and pT3 (n = 8) and 85% for pT1 patients (n = 20). The intensity of peaks A and C continuously decreased from pT1 to pT3, whereas peak B increased in pT1 and pT2.


CONCLUSIONS
These results may be useful to set up new diagnostic or prognostic tools.
Chinello
Clizia
C
Department of Experimental Medicine, University of Milano-Bicocca, Monza, Italy.
Gianazza
Erica
E
Zoppis
Italo
I
Mainini
Veronica
V
Galbusera
Carmen
C
Picozzi
Stefano
S
Rocco
Francesco
F
Galasso
Giacomo
G
Bosari
Silvano
S
Ferrero
Stefano
S
Perego
Roberto
R
Raimondo
Francesca
F
Bianchi
Cristina
C
Pitto
Marina
M
Signorini
Stefano
S
Brambilla
Paolo
P
Mocarelli
Paolo
P
Galli Kienle
Marzia
M
Magni
Fulvio
F
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods*
D014408:Biomarkers, Tumor