[Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with vectors bearing genes of bacterial antigenes from Mycobacterium tuberculosis].
Transgenic plants of lettuce Lactuca sativa L. cv. Eralash, Sniezinka, Rubinovoje kruzevo with genes coding synthesis of tuberculosis antigenes have been obtained by Agrobacterium-mediated transformation. Cotyledons of in vitro seedlings were used as the initial material for transformation with plasmids pCB063 (genes ESAT6, nptII) and pCB064 (genes ESAT6:AG85B(-TMD), nptII). PCR-analysis has shown the presence both selective and target genes in all plants analyzed. At the same time, the RT-PCR has shown that both the presence and the absence of a transcription of gene ESAT6 at a stable transcription of a gene nptII is possible.
Marveeva
N A
NA
Vasilenko
M Iu
MIu
Shakhovskiĭ
A M
AM
Kuchuk
N V
NV
D000942:Antigens, Bacterial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005818:Genetic Engineering; D005822:Genetic Vectors; D018545:Lactuca / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012038:Regeneration; D014170:Transformation, Genetic; D032581:Tuberculosis Vaccines / Q000235:genetics
D000942:Antigens, Bacterial; D018744:DNA, Plant; D032581:Tuberculosis Vaccines