Lipidomic analysis reveals prostanoid profiles in human term pregnant myometrium.
Prostanoids modulate the activity of human pregnant myometrium and their functional role can be appreciated through characterisation of prostanoid receptors and tissue concentration of prostanoids. We have applied a lipidomic approach to elucidate the profile of prostanoids in human non-labouring and labouring myometrium. We have identified a total of nineteen prostanoids including prostacyclin, thromboxanes, prostaglandins and dihydro-prostaglandins. Prostacyclin was the predominant prostanoid in both non-labouring and labouring myometria, with PGD(2) and PGF(2alpha) being the second most abundant. Although the total amount of prostanoids was increased in the labouring tissue, PGE(2) and 13,14-dihydro-15-keto-PGE(2) were the only prostanoids to increase significantly at early and late labour (p< or =0.001). Our data suggest that PGF(2alpha) plays an important role in parturition, whilst the increase in PGE(2) could occur to facilitate cervical dilation and relaxation of the lower myometrium during labour. Although the elevation in TXA(2) was less marked than expected, in terms of translation to function even a relatively small increase in the level of this potent spasmogen may have significant effects.
Durn
J H
JH
School of Pharmacy, University of Bradford, Bradford BD7 1DP, UK.
Marshall
K M
KM
Farrar
D
D
O'Donovan
P
P
Scally
A J
AJ
Woodward
D F
DF
Nicolaou
A
A
D002585:Cesarean Section; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007747:Labor Stage, First; D007743:Labor, Obstetric; D009215:Myometrium / Q000737:chemistry*; D011247:Pregnancy; D011453:Prostaglandins / Q000032:analysis*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D047929:Term Birth; D014590:Uterine Contraction
D011453:Prostaglandins