title: Segment-2 sequence analysis and cross-neutralization studies on some Indian bluetongue viruses suggest isolates are VP2-variants of serotype 23.
abstract: Sequence analysis of segment 2 (seg-2) of three Indian bluetongue virus (BTV) isolates, Dehradun, Rahuri and Bangalore revealed 99% nucleotide identity amongst them and 96% with the reference BTV 23. Phylogenetic analysis grouped the isolates in 'nucleotype D'. The deduced amino acid (aa) sequence of the Bangalore isolate showed a high variability in a few places compared to other isolates. B-cell epitope analyses predicted an epitope that is present exclusively in the Bangalore isolate. Two-way cross serum neutralization confirmed that Bangalore isolate is antigenically different from the other two isolates. The results of this study suggest that these three isolates are VP2 variants of BTV 23. This signifies that non-cross-neutralizing variants of the same BTV serotype should be included in vaccine preparation.
authors: National AIDS Research Center, Bhosari, Pune 411026, Maharashtra, India.
authors: Prabhakar A
authors: PA
authors: Tembhurne
authors: Bimalendu
authors: B
authors: Mondal
authors: Kunj B
authors: KB
authors: Pathak
authors: Sanchay K
authors: SK
authors: Biswas
authors: Aniket
authors: A
authors: Sanyal
authors: Mahendra P
authors: MP
authors: Yadav
authors: Santanu K
authors: SK
authors: Bandyopadhyay
authors: Raj K
authors: RK
authors: Singh
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000276:immunology / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012756:Sheep
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C442520:VP2 protein, Bluetongue virus