Multiplex microsphere-based flow cytometric platforms for protein analysis and their application in clinical proteomics - from assays to results.
Advances in high-throughput screening, together with rapid progress in genomic and proteomic sciences, have considerably stimulated the development of a variety of biomarker discovery tools and have contributed to the improvement of clinical diagnosis. Major challenges include the obtaining of high-quality data sets based on assays that are rapid, reliable, and inexpensive, but still highly sensitive and easy to perform. The microsphere-based flow cytometric technology is a platform that is currently entering the diagnostic market. This article reviews the development and the applications of microsphere-based flow cytometric platforms in basic and applied proteomic research, and discusses bead-based assays used in clinical applications.
Hsu
Hsin-Yun
HY
Laboratory of Medical Genomics, Department of Human Genome Research, Kazusa DNA Research Institute, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. hsinyun@kazusa.or.jp
Joos
Thomas O
TO
Koga
Hisashi
H
D019411:Clinical Laboratory Techniques / Q000379:methods*; D055808:Drug Discovery; D005434:Flow Cytometry / Q000379:methods*; D008863:Microspheres; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*