Allergenicity of Hev b 13, a major esterase allergen in natural rubber latex (Hevea brasiliensis) allergy, does not only depend on its carbohydrate moiety.
The three-dimensional model built for the major latex allergen Hev b 13 consists of the typical organization of plant esterases made of a central bundle of five parallel beta-strands surrounded by five alpha-helices associated to two shorter alpha-helical segments. Up to 12 sets of sequential IgE-binding peptides were identified in SPOT experiments along the amino acid sequence of Hev b 13. They correspond in fact to eight IgE-binding epitopic stretches exposed on the surface of the allergen. With the exception of epitope #5, all other epitopes contain charged residues. Epitope #8 contains the 3rd putative N-glycosylation site of Hev b 13 and should consist of a glycotope, whereas all other identified IgE-binding areas occur outside the two remaining putative N-glycosylation sites. Accordingly, the allergenicity of Hev b 13 does not primarily depends on its carbohydrate moiety.
Rougé
Pierre
P
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR Université Paul Sabatier-CNRS 5546, 24 Chemin de Borde Rouge, 31326 Castanet Tolosan, France. rouge@scsv.ups-tlse.fr
Culerrier
Raphaël
R
Campistron
Marion
M
Granier
Claude
C
Bienvenu
Françoise
F
Bienvenu
Jacques
J
Didier
Alain
A
Barre
Annick
A
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant; D002241:Carbohydrates / Q000276:immunology*; D002417:Cattle; D002648:Child; D018604:Epitope Mapping; D004950:Esterases / Q000276:immunology*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D007840:Latex / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology*; D017433:Protein Structure, Secondary; D012408:Rubber / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D055815:Young Adult
D000485:Allergens; D052179:Antigens, Plant; D002241:Carbohydrates; C473106:Hev b 13 allergen, Hevea brasiliensis; D007840:Latex; D010940:Plant Proteins; D007073:Immunoglobulin E; D012408:Rubber; D004950:Esterases