Selection of RNA aptamers specific to active prostate-specific antigen.
A counter-SELEX procedure with recombinant purified active prostate specific antigen (PSA) was used to identify specific RNA aptamers against the active PSA. We developed two different kinds of counter-SELEX methods; one includes pre-clearance step with inactive proPSA protein, and the other with tagged GST protein. After 9 iterative selection cycles, several identical RNA aptamers can be identified from both counter-SELEX methods. Real-time PCR analysis and gel retardation experiment showed that the aptamers have a specific binding activity against the active PSA, but not for GST or proPSA. These aptamers could be of potential use as specific diagnostic, imaging and/or therapeutic agents against prostate cancer.
Jeong
Sujin
S
Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, 126, Jukjeon-dong, Suji-gu, Yongin, 448-701, Korea.
Han
Seung Ryul
SR
Lee
Young Ju
YJ
Lee
Seong-Wook
SW
D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002999:Clone Cells; D006801:Humans; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011485:Protein Binding; D052156:SELEX Aptamer Technique / Q000379:methods*
D052157:Aptamers, Nucleotide; D017430:Prostate-Specific Antigen