[Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01].
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from beta-D-mannuronic acids and alpha-L-gluronic acids via beta-(1-->4)/ alpha-(1-->4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
Guo
Wenbin
W
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Wang
Shufang
S
Cao
Mingfeng
M
Geng
Weitao
W
Song
Cunjiang
C
D000464:Alginates / Q000737:chemistry; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006603:Hexuronic Acids / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D044203:Pseudomonas mendocina / Q000378:metabolism*
D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid