title: Saccharomyces cerevisiae and Candida albicans stimulate cytokine secretion from human neutrophil-like HL-60 cells differentiated with retinoic acid or dimethylsulfoxide.
abstract: We investigated whether non-pathogenic Saccharomyces cerevisiae and human commensal opportunistic pathogenic Candida albicans stimulate cytokine responses of human neutrophil-like HL-60 cells pre-treated with either 1 microM retinoic acid or 1.25% dimethyl sulfoxide (DMSO). Intact and heat-killed S. cerevisiae enhanced secretion of interleukin (IL)-1beta, IL-6, IL-8, IL-12, IL-18, MCP-1/CCL2 and TNF-alpha from retinoic acid-treated HL-60 cells, accompanied by alterations in mRNA expression of the cytokines. Heat-killed C. albicans promoted secretion of IL-6, IL-8, IL-12, MCP-1 and TNF-alpha, while intact C. albicans slightly enhanced secretion of IL-1beta, IL-8 and IL-18. In response to yeast stimuli, retinoic acid-treated HL-60 cells generally secreted cytokines more strongly than DMSO-treated HL-60 cells. Gene expression levels of Toll-like receptor (TLR)1, TLR2, TLR4, TLR6 and dectin-1 in HL-60 cells were additionally affected by retinoic acid or DMSO and by co-culturing with S. cerevisiae or C. albicans. Our results suggest that both intact and heat-killed S. cerevisiae and C. albicans induce cytokine responses of neutrophils in the intestine, and stimulate host immune function.
authors: Tokyo Metropolitan Food Technology Research Center, Japan. shizue-saegusa@food-tokyo.jp
authors: Shizue
authors: S
authors: Saegusa
authors: Mamoru
authors: M
authors: Totsuka
authors: Shuichi
authors: S
authors: Kaminogawa
authors: Tomohiro
authors: T
authors: Hosoi
mesh_terms: D002176:Candida albicans / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D018922:HL-60 Cells; D006358:Hot Temperature; D006801:Humans; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000276:immunology* / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D000099181:Dectin-1
chemical_list: D016207:Cytokines; D004121:Dimethyl Sulfoxide; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D051193:Toll-Like Receptors; D014212:Tretinoin; D000099181:Dectin-1