[Study on polymorphism of UCP2 gene in Chengdu simple obesity and normal-weight people and a preliminary investigation of its relationship with gut bacteria].
OBJECTIVE
To study on the polymorphism of UCP2 gene in Chengdu simple obesity and normal-weight people and to initially investigate the relationship between UCP2 Ala55Val variation and gut bacteria.


METHODS
PCR-PFLP was applied to determine the genotypes of Ala55Val variant in the UCP2 gene of 86 Chengdu people (the simple obesity group, 43 subjects; the normal-weight group, 43 subjects). And six kinds of gut bacteria among different genotypes in different groups were analyzed.


RESULTS
Both the simple obesity and the normal-weight group had the Ala55Val variants of Ala/Ala, Val/Val and Ala/Val in the UCP2 gene, and the Ala55Val genotype distributions between the two groups was significantly different (chi2=11.97, P< 0.05). The allelic mutation frequency in the simple obesity group was higher than that of the normal-weight group (chi2=10.06, P<0.05). However, no significant difference was observed in the population of six gut bacteria among different genotypes in different groups (P>0.05).


CONCLUSIONS
The UCP2 gene mutation might be a risk factor of obesity in Chengdu area. However, this gene mutation may not be an impact factor on the alternation of gut bacteria.
Zuo
Hao-Jiang
HJ
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu, China.
Zhang
Wei-Wei
WW
Xie
Zhi-Mei
ZM
Li
Yong-Ru
YR
Wang
Wei
W
Ding
Xiao-Bei
XB
Pei
Xiao-Fang
XF
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002681:China; D005260:Female; D005838:Genotype; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D009154:Mutation; D009765:Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors; D000071246:Uncoupling Protein 2; D055815:Young Adult
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2