title: Proteomic analysis of laser-microdissected paraffin-embedded tissues: (1) Stage-related protein candidates upon non-metastatic lung adenocarcinoma.
abstract: We used formalin-fixed paraffin-embedded (FFPE) materials for biomarker discovery in cases of lung cancer using proteomic analysis. We conducted a retrospective global proteomic study in order to characterize protein expression reflecting clinical stages of individual patients with stage I lung adenocarcinoma without lymph node involvement (n=7). In addition, we studied more advanced stage IIIA with spread to lymph nodes (n=6), because the degree of lymph node involvement is the most important factor for staging. FFPE sections of cancerous lesions resected surgically from patients with well-characterized clinical history were subjected to laser microdissection (LMD) followed by Liquid Tissue solubilization and digestion trypsin. Spectral counting was used to measure the amounts of proteins identified by shotgun liquid chromatography (LC)/tandem mass spectrometry (MS/MS). More than 500 proteins were identified from IA and IIIA cases, and non-parametric statistics showed that 81 proteins correlated significantly with stage IA or IIIA. A subset of those proteins were verified by multiple-reaction monitoring mass spectrometric quantitation (MRM assay), described in other paper in this issue. These results demonstrated the technical feasibility of a global proteomic study using clinically well documented FFPE sections, and its possible utility for detailed retrospective disease analyses in order to improve therapeutic strategy.
authors: Laboratory for Systems Biology and Medicine, RCAST, The University of Tokyo, Tokyo, Japan.
authors: Takeshi
authors: T
authors: Kawamura
authors: Masaharu
authors: M
authors: Nomura
authors: Hiromasa
authors: H
authors: Tojo
authors: Kiyonaga
authors: K
authors: Fujii
authors: Hiroko
authors: H
authors: Hamasaki
authors: Sayaka
authors: S
authors: Mikami
authors: Yasuhiko
authors: Y
authors: Bando
authors: Harubumi
authors: H
authors: Kato
authors: Toshihide
authors: T
authors: Nishimura
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology / Q000379:methods*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007834:Lasers; D008175:Lung Neoplasms / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D010232:Paraffin / Q000737:chemistry*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D020543:Proteome; D010232:Paraffin