title: The herpes simplex virus-1 encoded glycoprotein B diverts HLA-DR into the exosome pathway.
abstract: Neutralizing Abs play an important role for immunity against HSV-1 infection. This branch of the immune response is initiated by MHC class II Ag presentation and activation of T cell help. In this study, we show that the HSV-1 encoded glycoprotein B (gB) manipulates the class II processing pathway by perturbing endosomal sorting and trafficking of HLA-DR (DR) molecules. Expression of gB in the human melanoma cell line Mel JuSo results in formation of enlarged DR(+) intracellular vesicles. Costaining of the vesicles revealed the presence of DR, gB, and the late endosomal marker CD63. The lumen of these late endosomal membranes shows a variable content, containing either gB or CD63, or both CD63 and gB. gB targets DR molecules on their biosynthetic route, after the MHC class II invariant chain is released from the DR heterodimer. gB-DR complexes were detected in a post-Golgi compartment and in exosomes, but not on the cell surface. Interestingly, increasing expression of gB strongly elevated the amount of DR and CD63 released into the exosome pathway. In conclusion, this is a previously undescribed mode of viral immune evasion involving hijacking of DR from its normal transport route to the cell surface, followed by viral-mediated release of DR into the exosome pathway.
authors: Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, Germany.
authors: Sebastian
authors: S
authors: Temme
authors: Anna M
authors: AM
authors: Eis-Hübinger
authors: Alexander D
authors: AD
authors: McLellan
authors: Norbert
authors: N
authors: Koch
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D055354:Exosomes / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism*; D006561:Herpes Simplex / Q000276:immunology* / Q000378:metabolism; D018259:Herpesvirus 1, Human; D006801:Humans; D057131:Immune Evasion; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D060149:Tetraspanin 30; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C554586:CD63 protein, human; D006684:HLA-DR Antigens; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus