title: [CpG-oligodeoxynucleotide stimulation improves the success for karyotypic analysis of chronic lymphocytic leukemia cells].
abstract: OBJECTIVE
  To explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome study of chronic lymphocytic leukemia (CLL).
  
  
  METHODS
  Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed.
  
  
  RESULTS
  The metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%.
  
  
  CONCLUSIONS
  CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.
authors: Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors: Qiong
authors: Q
authors: Liu
authors: Wei
authors: W
authors: Xu
authors: Hai-rong
authors: HR
authors: Qiu
authors: Rong
authors: R
authors: Wang
authors: Hui
authors: H
authors: Yu
authors: Lei
authors: L
authors: Fan
authors: Kou-rong
authors: KR
authors: Miao
authors: Jian-yong
authors: JY
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009841:Oligonucleotides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C539572:CpG-ODN DSP30; D009841:Oligonucleotides