Anti-tumor effects of AMT in the renal cell carcinoma model.
Auron-Misheil-Therapy (AMT) is a defined but unique combination of approved pharmaceuticals. It consists of insulin, chlorpheniramine and an aqueous camomile extract, and it has been successfully applied clinically in late-stage cancer patients. The purpose of this study was to elucidate the anti-tumor efficacy of AMT in a validated murine renal cell carcinoma animal model (RENCA). There were two independent studies; each animal group consisted of 16 mice. During a 6-week pretreatment period, vehicle (group A) and AMT (1.6 mg/kg/d) (group B) were administered once daily in a 5 days/week schedule either intramuscularly or subcutaneously. Tumor challenge at day 0 was followed by a 3-week treatment period (either vehicle or AMT once daily intramuscularly for 21 days consecutively). In study 2 the AMT dosage was increased up to 4-fold by doubling individual doses and switching to a twice daily schedule. The injections were all intramuscular. With the exception of group D, a six-week pretreatment period preceded the tumor challenge at day 0. Tumor challenge was followed by a 3-week treatment period (vehicle, AMT at either 3.2 mg/kg/d) (group A) or 6.4 mg/kg/d (group B), or AMT0, an AMT preparation which does not stimulate IL-6 secretion (6.4 mg/kg/d, group C) continuously for 21 days. AMT administration for group D (6.4 mg/kg/d) was limited to the treatment period from day 1 to 21. All mice were sacrificed 21 days after tumour transplantation. AMT administration was safe and well tolerated, and significantly reduced primary tumor volume in pretreated animals. The effective route of application was intramuscular, with dose escalation resulting in an improved anti-tumor effect. This is the first demonstration of a significant anti-tumorigenic effect of AMT in a validated tumor model.
Caballero
Marcello
M
Cancer Hospital Sanafontis, Freiburg, Germany.
Scheele
Jürgen
J
Zirrgiebel
Ute
U
Esser
Norbert
N
Schächtele
Christoph
C
Soltau
Jens
J
Rentschler
Jochen
J
Diergarten
Klaus
K
Drevs
Joachim
J
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002118:Calcium / Q000627:therapeutic use*; D002292:Carcinoma, Renal Cell / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002744:Chlorpheniramine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D007328:Insulin / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000378:metabolism; D007680:Kidney Neoplasms / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010936:Plant Extracts / Q000627:therapeutic use*; D013997:Time Factors
D000970:Antineoplastic Agents; C542427:Auron-Misheil-Therapy; D007328:Insulin; D015850:Interleukin-6; D010936:Plant Extracts; D002744:Chlorpheniramine; D002118:Calcium