Influenza A virus receptors in the respiratory and intestinal tracts of pigeons.
To understand the basis of the resistance of pigeons to Asian lineage highly pathogenic avian influenza virus (HPAIV) subtype H5N1, we examined the presence of influenza virus receptors, sialic acids linked to galactose by an alpha-2,3 linkage (SAalpha2,3Gal) or by an alpha-2,6 linkage (SAalpha2,6Gal), in respiratory and intestinal tracts of pigeons and compared the distributions of N-acetylneuraminic and N-glycolylneuraminic acids in the trachea and intestines of pigeons and chickens. Results suggested that the epithelial surfaces of the larynx, trachea, bronchus, and bronchiole of pigeons contained abundant SAalpha2,6Gal with little or no SAalpha2,3Gal. In contrast, the epithelial surfaces of the pharynx, trachea, bronchus, and bronchiole of chickens contained mainly SAalpha2,3Gal, a well-recognized receptor for avian influenza viruses including H5N1 HPAIV. A similar distribution pattern of N-acetylneuraminic and N-glycolylneuraminic acids in the trachea and intestines of pigeons and chickens was observed. Overall, the results suggest that SAalpha2,6Gal is the major receptor in the pigeon airway, which may partly contribute to the resistance of pigeons to Asian lineage HPAIV subtype H5N1.
Liu
Yuehuan
Y
Institute of Animal and Husbandry Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, PR China. liuyuehuan@baafs.net.cn
Han
Chunhua
C
Wang
Xiuqing
X
Lin
Jian
J
Ma
Ming
M
Shu
Yuelong
Y
Zhou
Jiao
J
Yang
Hanchun
H
Liang
Qian
Q
Guo
Chaotan
C
Zhu
Junjie
J
Wei
Haitao
H
Zhao
Jingyi
J
Ma
Zhijun
Z
Pan
Jie
J
D000818:Animals; D002645:Chickens / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D010856:Columbidae / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D007113:Immunity, Innate; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism / Q000472:pathogenicity; D005585:Influenza in Birds / Q000276:immunology / Q000378:metabolism / Q000821:virology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000821:virology; D037102:Lectins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009438:Neuraminic Acids / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D009928:Organ Specificity; D011991:Receptors, Virus / Q000378:metabolism*; D012137:Respiratory System / Q000378:metabolism* / Q000821:virology; D013045:Species Specificity
D037102:Lectins; D009438:Neuraminic Acids; D009844:Oligosaccharides; D011991:Receptors, Virus; C016735:sialooligosaccharides; C032592:N-glycolylneuraminic acid; D019158:N-Acetylneuraminic Acid