Adhesive properties and hydrolytic enzymes of oral Candida albicans strains.
Several virulence factors in Candida albicans strains such as production of hydrolytic enzymes and biofilm formation on surfaces and cells can contribute to their pathogenicity. For this, control of this opportunistic yeast is one of the factors reducing the nosocomial infection. The aim of this study was to investigate biofilm formation on polystyrene and polymethylmethacrylate and the production of hydrolytic enzymes in Candida albicans strains isolated from the oral cavity of patients suffering from denture stomatitis. All strains were identified by macroscopic, microscopic analysis and the ID 32 C system. Our results showed that 50% of the total strains produced phospholipase. Furthermore, protease activity was detected in seven (35%) strains. All Candida albicans strains were beta haemolytic. All C. albicans strains adhered to polystyrene 96-well microtiter plate at different degrees, and the metabolic activity of C. albicans biofilm formed on polymethylmethacrylate did not differ between tested strains. The atomic force micrographs demonstrated that biofilm of Candida albicans strains was organized in small colonies with budding cells.
Noumi
Emira
E
Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Monastir, Tunisie. emira_noumi@yahoo.fr
Snoussi
Mejdi
M
Hentati
Hajer
H
Mahdouani
Kacem
K
del Castillo
Lucas
L
Valentin
Eulogio
E
Sentandreu
Rafael
R
Bakhrouf
Amina
A
D000328:Adult; D000368:Aged; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000201:enzymology* / Q000302:isolation & purification / Q000502:physiology*; D002448:Cell Adhesion; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology; D010447:Peptide Hydrolases / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D013282:Stomatitis, Denture / Q000382:microbiology
D005656:Fungal Proteins; D010740:Phospholipases; D010447:Peptide Hydrolases