Propolis prevents diet-induced hyperlipidemia and mitigates weight gain in diet-induced obesity in mice.
We examined the hypolipidemic effect of propolis in a mouse obesity model induced by a high fat-diet. C57BL/6N mice were fed a high-fat diet ad libitum and given propolis extract intragastrically at 0 mg/kg (control), 5 mg/kg or 50 mg/kg twice daily for 10 d. Compared with mice in the control group, mice in the propolis extract-administrated groups displayed a reduction in all of the following parameters: body weight gain, weight of visceral adipose tissue, liver and serum triglycerides, cholesterol, and non-esterified fatty acids. Real-time polymerase chain reaction analysis of the liver showed down-regulation of mRNA expression associated with fatty acid biosynthesis, including fatty acid synthase, acetyl-CoA carboxylase alpha, and sterol regulatory element binding protein in the propolis-administrated mice. Subsequently, obese C57BL/6N mice that had been administered a high-fat diet were given propolis extract at 0 mg/kg (control), 2.5 mg/kg or 25 mg/kg for 4 weeks. The propolis extract treated mice showed a decrease in weight gain, a reduction of serum non-esterified fatty acids, and lipid accumulation in the liver. These results suggest that propolis extract prevented and mitigated high-fat diet-induced hyperlipidemia by down-regulating the expression of genes associated with lipid metabolism.
Koya-Miyata
Satomi
S
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan. fujisaki@hayashibara.co.jp
Arai
Norie
N
Mizote
Akiko
A
Taniguchi
Yoshifumi
Y
Ushio
Shimpei
S
Iwaki
Kanso
K
Fukuda
Shigeharu
S
D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D056705:Apitherapy; D001835:Body Weight / Q000187:drug effects*; D004032:Diet / Q000009:adverse effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D006949:Hyperlipidemias / Q000209:etiology / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D050152:Intra-Abdominal Fat / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy*; D011429:Propolis / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051779:Sterol Regulatory Element Binding Proteins / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects*
D004041:Dietary Fats; D000960:Hypolipidemic Agents; D012333:RNA, Messenger; D051779:Sterol Regulatory Element Binding Proteins; D011429:Propolis; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase