[Transport mechanism of swertiamain metabolite across Caco-2 model].
OBJECTIVE
To investigate the intestinal absorption mechanism of swertiamain metabolite {(Z)-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano [3,4-c]pyridine-1-one, EHPO}.


METHODS
The time depended hi-directional transport of EHPO in Caco-2 monolayer model was investigated with the factors of concentration, pH and verapamil. EHPO concentration was measured by HPLC assay and the apparent permeability coefficients (Papp) were calculated.


RESULTS
In the this cell model, EHPO could be absorbed through Caco-2 soon. The P(app AP-BL) was equal to the P(app BL-AP), and Papp keep almost constant with the selected concentration investigated. But the Papp could be influenced by pH and verapamil (100 mg x L(-1)).


CONCLUSIONS
The absorption of EHPO in Caco-2 cell model is a passive one. And absorption of EHPO in the intestines is quite good.
Tang
Cheng
C
Pharmacy College, Tianjin Medical University, Tianjin 300070, China.
Zhang
Yanjun
Y
Zhang
Tiejun
T
Tian
Chengwang
C
Liu
Changxiao
C
D001692:Biological Transport; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008954:Models, Biological
D004365:Drugs, Chinese Herbal