[Karyotype analysis of chorionic villi from pregnant women with missed abortion using multiplex ligation-dependent probe amplification].
OBJECTIVE
To evaluate the clinical value of multiplex ligation-dependent probe amplification (MLPA) technique used in karyotype analysis of chorionic villi from missed abortion.


METHODS
Feb 2008 to Oct 2008, 91 patients with missed abortion diagnosed by hormonal measurement, type B ultrasound and physical exam matched with 20 normal pregnant women undergoing artificial abortion were enrolled in this study. Chorionic villi was obtained by suction dilation and curettage in aseptic condition, then those villi was cultured and analyzed by traditional cytogenetic karyotyping method, in the mean time, the DNA extracted from villi was detected by MLPA. The results of chromosomal G-banding of chorionic villi were compared between two methods.


RESULTS
The diagnostic concordance of MLPA and traditional karyotyping was observed in 92% (84/91) cases, there were 84 cases in the case group with diagnostic concordance by traditional karyotyping and MLPA except 7 cases of euploidy could not be detected by MLPA. The 84 cases included 40 normal karyotype, 29 trisomy of euchromosome, 1 double trisomy of euchromosome, 10 monosomy X, 1 monosomy X combined with trisomy of euchromosome, 2 chimaera of X chromosome, 1 structural abnormity of euchromosome. Among 7 cases with discordance diagnosis, 2 cases with trisomy and 5 cases with tetrasomy of euchromosome were identified in traditional karyotyping, however, they were all diagnosed with normal disomy by MLPA. Of 20 villi from normal pregnancy, two methods got the consistent results.


CONCLUSIONS
The MLPA was rapid and efficacy method used for analyzing aneuploids in chorionic villi.
Gu
Yan
Y
Center for prenatal Diahgnosis, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, China.
Xie
Jian-sheng
JS
Luo
Fu-wei
FW
Geng
Qian
Q
Zhang
Hua-kun
HK
Shen
Hui-ning
HN
Zhao
Kun
K
Liu
Qing-zhi
QZ
D000030:Abortion, Missed / Q000235:genetics*; D000328:Adult; D015193:Chorionic Villi Sampling / Q000379:methods*; D002869:Chromosome Aberrations; D003582:Cytogenetics; D005260:Female; D006801:Humans; D007621:Karyotyping; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D012680:Sensitivity and Specificity; D014314:Trisomy / Q000235:genetics; D055815:Young Adult