title: Separating stem cells by flow cytometry: reducing variability for solid tissues.
abstract: Until there are valid identifiers that visualize stem cells in vivo, we rely upon flow cytometry to enrich for subpopulations with stem cell function. However, data reporting styles for flow cytometric analyses are typically inconsistent, creating challenges in comparing results across publications. In our view, clear reporting guidelines could improve reproducibility of stem cell analyses in solid tissues.
authors: McArdle Laboratory for Cancer Research, Madison, WI 53706, USA. alexander@oncology.wisc.edu
authors: Caroline M
authors: CM
authors: Alexander
authors: Joel
authors: J
authors: Puchalski
authors: Kristine S
authors: KS
authors: Klos
authors: Nisha
authors: N
authors: Badders
authors: Laurie
authors: L
authors: Ailles
authors: Carla F
authors: CF
authors: Kim
authors: Peter
authors: P
authors: Dirks
authors: Matthew J
authors: MJ
authors: Smalley
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D017410:Practice Guidelines as Topic / Q000592:standards; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology