Regression of murine lung tumors by the let-7 microRNA.
MicroRNAs (miRNAs) have recently emerged as an important new class of cellular regulators that control various cellular processes and are implicated in human diseases, including cancer. Here, we show that loss of let-7 function enhances lung tumor formation in vivo, strongly supporting the hypothesis that let-7 is a tumor suppressor. Moreover, we report that exogenous delivery of let-7 to established tumors in mouse models of non-small-cell lung cancer (NSCLC) significantly reduces the tumor burden. These results demonstrate the therapeutic potential of let-7 in NSCLC and point to miRNA replacement therapy as a promising approach in cancer treatment.
Trang
P
P
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Medina
P P
PP
Wiggins
J F
JF
Ruffino
L
L
Kelnar
K
K
Omotola
M
M
Homer
R
R
Brown
D
D
Bader
A G
AG
Weidhaas
J B
JB
Slack
F J
FJ
D000818:Animals; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000008:administration & dosage / Q000235:genetics*; D016372:RNA, Antisense / Q000008:administration & dosage / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
D035683:MicroRNAs; D016372:RNA, Antisense; C487931:mirnlet7 microRNA, human