title: Floxin, a resource for genetically engineering mouse ESCs.
abstract: We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
authors: Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors: Veena
authors: V
authors: Singla
authors: Julie
authors: J
authors: Hunkapiller
authors: Nicole
authors: N
authors: Santos
authors: Allen D
authors: AD
authors: Seol
authors: Andrew R
authors: AR
authors: Norman
authors: Paul
authors: P
authors: Wakenight
authors: William C
authors: WC
authors: Skarnes
authors: Jeremy F
authors: JF
authors: Reiter
mesh_terms: D000483:Alleles; D000818:Animals; D001287:Attachment Sites, Microbiological / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D020224:Expressed Sequence Tags; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers; D005822:Genetic Vectors; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation
chemical_list: D005819:Genetic Markers; C045073:Cre recombinase; D019426:Integrases