Telomerase and HER-2/neu as targets of genetic cancer vaccines in dogs.
Pet dogs represent a valuable pre-clinical model to assess the efficacy of oncology drugs. Additionally, canine cancers occur with an incidence similar to that of humans and share many features with human malignancies including histological appearance, tumor genetics, biological behavior and response to conventional therapies. The telomerase reverse transcriptase (TERT) is reactivated in most of human and dog tumors. Similarly, HER-2/neu oncoprotein is overexpressed in a proportion of canine breast cancers. Therefore, TERT and HER-2/neu can constitute valid tumor associated antigens (TAA), suitable targets for translational cancer immunotherapy in dogs. In this study, we have evaluated the ability of DNA electroporation (DNA-EP) and Adenovirus serotype 6 (Ad6) to induce immune responses against dog TERT (dTERT) and HER-2/neu in healthy dogs. Vaccination was effective in all treated animals and the adaptive immune response remained detectable and long-lasting in the absence of autoimmunity or other side-effects. Our results show that DNA-EP/Ad6-based cancer vaccine induces adaptive immune responses against TAA in canine subjects and support further evaluation of this approach in cancer dog patients.
Peruzzi
Daniela
D
Oncology Department, IRBM - Istituto di Ricerche di Biologia Molecolare, Via Pontina Km 30.600, 00040 Pomezia, Rome, Italy.
Mesiti
Giuseppe
G
Ciliberto
Gennaro
G
La Monica
Nicola
N
Aurisicchio
Luigi
L
D056704:Adaptive Immunity; D000256:Adenoviridae; D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology*; D004283:Dog Diseases; D004285:Dogs; D018274:Electroporation; D005260:Female; D006801:Humans; D007114:Immunization; D015674:Mammary Neoplasms, Animal / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology*; D019098:Telomerase
D019496:Cancer Vaccines; D018719:Erb-b2 Receptor Tyrosine Kinases; D019098:Telomerase; C508053:ERBB2 protein, human