title: [Mechanism of cardioprotection induced by noninvasive limb ischemic preconditioning].
abstract: OBJECTIVE
  To verify the inhibitory effect of mitochondrial calcium uniporter in remote preconditioning-induced cardioprotection.
  
  
  METHODS
  By occlusion and reperfusion of left anterior descending artery, the rat hearts were subjected to 30 min regional ischemia and 120 min reperfusion in vivo. Thus the ischemic reperfusion model was established. The rats were randomly assigned to undergo one of the following maneuvers: (1) remote preconditioning; (2) ruthenium red (an inhibitor of mitochondrial calcium uniporter); (3) spermine or SB202190 (an opener of mitochondrial calcium uniporter). Remote preconditioning was elicited by three cycles of 5 min of right femoral artery occlusion interspersed with 5 min of reperfusion. The mean arterial blood pressure, heart rate and lactate dehydrogenase released in plasma were measured during reperfusion but the infarct size was measured after reperfusion.
  
  
  RESULTS
  In comparison with I/R group, remote preconditioning limited infarct size [(20.4 +/- 2.5)% vs (51.0 +/- 6.0)%] and lactate dehydrogenase release [(271 +/- 9) U/L vs (339 +/- 39)U/L] during reperfusion. On the contrary, spermine or SB202190 attenuated the reduction of infarct size and lactate dehydrogenase release induced by remote preconditioning. The group of spermine was [(40.8 +/- 9.2)% vs (20.4 +/- 2.5)%] and [(383 +/- 43) U/L vs (271 +/- 9) U/L] while the group of SB202190 was [(44.3 +/- 6.8)% vs (20.4 +/- 2.5)%] and [(356 +/- 26) U/L vs (271 +/- 9) U/L].
  
  
  CONCLUSIONS
  Inhibition of mitochondrial calcium uniporter opening is involved in the remote preconditioning-induced cardioprotection.
authors: Affiliated Hangzhou Hospital of Nanjing Medical University, Department of Cardiology, First People's Hospital of Hangzhou, Hangzhou 310006, China. Wangningfu@hotmail.com
authors: Ning-Fu
authors: NF
authors: Wang
authors: Jian
authors: J
authors: Xu
authors: Qiang
authors: Q
authors: Xia
authors: Qin
authors: Q
authors: Gao
authors: Xiao-Xiao
authors: XX
authors: Shi
authors: Zhan-Lin
authors: ZL
authors: Zhou
authors: Lin-Lin
authors: LL
authors: Wang
authors: Guo-Xin
authors: GX
authors: Tong
authors: Hong
authors: H
authors: Li
authors: Pei-Zhang
authors: PZ
authors: Li
authors: Xiong
authors: X
authors: Zhang
authors: Feng
authors: F
authors: Ling
authors: Hao
authors: H
authors: Pan
authors: Jian-Min
authors: JM
authors: Yang
mesh_terms: D000818:Animals; D015220:Calcium Channels; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D008929:Mitochondria, Heart; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015220:Calcium Channels; C034703:mitochondrial calcium uniporter