Protein expression profiling of primary mammary epithelial cells derived from MMTV-neu mice revealed that HER2/NEU-driven changes in protein expression are functionally clustered.
MMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis.
Park
Sungwoo
S
Department of Life Science, Hanyang University, Seoul 133-791, Korea.
Lee
Kyung-min
KM
Ju
Ji-hyun
JH
Kim
Jaeyoon
J
Noh
Dong-Young
DY
Lee
Taehoon
T
Shin
Incheol
I
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism*; D008324:Mammary Tumor Virus, Mouse; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D040081:Protein Array Analysis; D040901:Proteomics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
D018719:Erb-b2 Receptor Tyrosine Kinases; C524056:Erbb2 protein, mouse