Diagnostic assay for Rickettsia japonica.
We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica-related strains.
Hanaoka
Nozomu
N
National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Matsutani
Minenosuke
M
Kawabata
Hiroki
H
Yamamoto
Seigo
S
Fujita
Hiromi
H
Sakata
Akiko
A
Azuma
Yoshinao
Y
Ogawa
Motohiko
M
Takano
Ai
A
Watanabe
Haruo
H
Kishimoto
Toshio
T
Shirai
Mutsunori
M
Kurane
Ichiro
I
Ando
Shuji
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000175:diagnosis