title: Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo.
abstract: BACKGROUND
  Normal gastrointestinal motility requires intact networks of interstitial cells of Cajal (ICC). Interstitial cells of Cajal numbers are maintained by a balance between cell loss factors and survival/trophic/growth factors. Activation of 5-HT(2B) receptors expressed on ICC increases ICC proliferation in vitro. It is not known whether 5-HT(2B) receptors on ICC are activated in vivo. The aims of this study were to investigate if adult ICC proliferate, whether the proliferation of ICC in vivo is affected by knocking out the 5-HT(2B) receptor, and if alterations in proliferation affect ICC networks.
  
  
  METHODS
  Proliferating ICC were identified by immunoreactivity for Ki67 in both the myenteric and deep muscular plexus regions of the jejunum in mice with a targeted insertion of a neomycin resistance cassette into the second coding exon of the htr2b receptor gene.
  
  
  RESULTS
  Adult ICC do proliferate. The number of proliferating ICC was lower in the myenteric plexus region of Htr2b(-/-) compared to Htr2b(+/+) mice. The volume of Kit-positive ICC was 30% lower in the myenteric plexus region and 40% lower in the deep muscular plexus region in Htr2b(-/-) mice where the number of ICC was also reduced.
  
  
  CONCLUSIONS
  Interstitial cells of Cajal proliferate in adult mice and activation of 5-HT(2B) receptors results in increased proliferation of ICC in vivo. Furthermore, lack of 5-HT(2B) receptor signaling reduces the density of ICC networks in mature mice. These data suggest that 5-HT(2B) receptor signaling is required for maintenance of ICC networks, adding 5-HT to the growing number of factors shown to regulate ICC networks.
authors: Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic, Rochester, MN 55905, USA.
authors: V S
authors: VS
authors: Tharayil
authors: M M
authors: MM
authors: Wouters
authors: J E
authors: JE
authors: Stanich
authors: J L
authors: JL
authors: Roeder
authors: S
authors: S
authors: Lei
authors: A
authors: A
authors: Beyder
authors: P J
authors: PJ
authors: Gomez-Pinilla
authors: M D
authors: MD
authors: Gershon
authors: L
authors: L
authors: Maroteaux
authors: S J
authors: SJ
authors: Gibbons
authors: G
authors: G
authors: Farrugia
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005772:Gastrointestinal Transit / Q000235:genetics; D007150:Immunohistochemistry; D056885:Interstitial Cells of Cajal / Q000378:metabolism*; D007583:Jejunum / Q000294:innervation / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D042282:Microdissection / Q000379:methods; D018613:Microscopy, Confocal; D009197:Myenteric Plexus / Q000378:metabolism / Q000502:physiology*; D009415:Nerve Net / Q000378:metabolism / Q000502:physiology*; D009473:Neuronal Plasticity / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D044403:Receptor, Serotonin, 5-HT2B / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D044403:Receptor, Serotonin, 5-HT2B; D019009:Proto-Oncogene Proteins c-kit