Antibodies against structural and nonstructural proteins of human bocavirus in human sera.
Immunofluorescence assays (IFAs) for detection of human bocavirus (HBoV) proteins (VP1, VP2, NP-1, and NS1) were developed. The VP1 IFA was the most sensitive for detection of IgG antibody and suitable for screening. IgG antibodies in convalescent-phase sera from HBoV-positive patients were detected by VP1 and VP2 IFAs. Sensitivities of NP-1 and NS1 IFAs were low.
Shirkoohi
Reza
R
Department of Pediatrics, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan.
Endo
Rika
R
Ishiguro
Nobuhisa
N
Teramoto
Shinobu
S
Kikuta
Hideaki
H
Ariga
Tadashi
T
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D056661:Human bocavirus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000175:diagnosis*; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D055815:Young Adult
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins