Effects of ceftobiprole and oxacillin on mecA expression in methicillin-resistant Staphylococcus aureus clinical isolates.
Induction of mecA by ceftobiprole and oxacillin in 18 methicillin-resistant Staphylococcus aureus clinical isolates with various SCCmec cassettes was examined using reverse transcriptase PCR. The magnitude of mecA induction, 3- to 65-fold for ceftobiprole and 2- to 69-fold for oxacillin, did not correlate with ceftobiprole MICs (<or=4 microg/ml), although the 11 isolates with at least 13-fold induction by oxacillin all had oxacillin MICs of >or=256 microg/ml. No correlation between magnitude of induction and SCCmec type was found.
Shang
Wenchi
W
Johnson & Johnson Pharmaceutical Research & Development LLC, 1000 Route 202 South, Raritan, NJ 08869, USA. wshang2@its.jnj.com
Davies
Todd A
TA
Flamm
Robert K
RK
Bush
Karen
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D010068:Oxacillin / Q000494:pharmacology*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; C443755:ceftobiprole; D010068:Oxacillin