title: Metabolic alterations in the hamster co-infected with Schistosoma japonicum and Necator americanus.
abstract: Co-infection with hookworm and schistosomes is a common phenomenon in sub-Saharan Africa, as well as in parts of South America and southeast Asia. As a first step towards understanding the metabolic response of a hookworm-schistosome co-infection in humans, we investigated the metabolic consequences of co-infection in an animal model, using a nuclear magnetic resonance (NMR)-based metabolic profiling technique, combined with multivariate statistical analysis. Urine and serum samples were obtained from hamsters experimentally infected with 250 Necator americanus infective L(3) and 100 Schistosoma japonicum cercariae simultaneously. In the co-infection model, similar worm burdens were observed as reported for single infection models, whereas metabolic profiles of co-infection represented a combination of the altered metabolite profiles induced by single infections with these two parasites. Consistent differences in metabolic profiles between the co-infected and non-infected control hamsters were observed from 4 weeks p.i. onwards. The predominant metabolic alterations in co-infected hamsters consisted of depletion of amino acids, tricarboxylic acid cycle intermediates (e.g. citrate and succinate) and glucose. Moreover, alterations of a series of gut microbial-related metabolites, such as decreased levels of hippurate, 3-hydroxyphenylpropionic acid, 4-hydroxyphenylpropionic acid and trimethylamine-N-oxide, and increased concentrations of 4-cresol glucuronide and phenylacetylglycine were associated with co-infection. Our results provide a first step towards understanding the metabolic response of an animal host to multiple parasitic infections.
authors: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
authors: Jun-Fang
authors: JF
authors: Wu
authors: Elaine
authors: E
authors: Holmes
authors: Jian
authors: J
authors: Xue
authors: Shu-Hua
authors: SH
authors: Xiao
authors: Burton H
authors: BH
authors: Singer
authors: Hui-Ru
authors: HR
authors: Tang
authors: Jürg
authors: J
authors: Utzinger
authors: Yu-Lan
authors: YL
authors: Wang
mesh_terms: D000818:Animals; D006224:Cricetinae; D009682:Magnetic Resonance Spectroscopy; D008647:Mesocricetus; D055442:Metabolome; D017221:Necator americanus / Q000254:growth & development*; D009332:Necatoriasis / Q000150:complications* / Q000503:physiopathology*; D012549:Schistosoma japonicum / Q000254:growth & development*; D012554:Schistosomiasis japonica / Q000150:complications* / Q000503:physiopathology*; D044967:Serum / Q000737:chemistry; D014556:Urine / Q000737:chemistry