title: Targeting Gene-ViroTherapy for prostate cancer by DD3-driven oncolytic virus-harboring interleukin-24 gene.
abstract: Prostate cancer (PCa) is the second leading cause of cancer-related deaths in Western male population. Previous studies have demonstrated that differential display code 3 (DD3 or DD3(PCA3)) is one of the most PCa-specific genes; therefore, it has been used as a clinical diagnostic marker for PCa. In this study, we constructed an oncolytic adenovirus Ad.DD3-E1A by using the minimal DD3 promoter to replace the native viral promoter of E1A gene. In addition, Ad.DD3-E1A was armed with therapeutic gene IL-24 to enhance its antitumor activity. The resulting adenovirus, Ad.DD3-E1A-IL-24, demonstrated PCa specificity and excellent antitumor effect. Further analyses on its antitumor mechanism revealed that it has the capacity to induce apoptosis in PCa cells and inhibit angiogenesis. These results suggest that Ad.DD3-E1A-IL-24 is a promising antitumor agent that may be able to be used in the future as a treatment for PCa.
authors: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors: Jun Kai
authors: JK
authors: Fan
authors: Na
authors: N
authors: Wei
authors: Miao
authors: M
authors: Ding
authors: Jin Fa
authors: JF
authors: Gu
authors: Xin Ran
authors: XR
authors: Liu
authors: Bing Hua
authors: BH
authors: Li
authors: Rong
authors: R
authors: Qi
authors: Wei Dan
authors: WD
authors: Huang
authors: Yu Hua
authors: YH
authors: Li
authors: Xiao Quan
authors: XQ
authors: Xiong
authors: Jian
authors: J
authors: Wang
authors: Run Sheng
authors: RS
authors: Li
authors: Xin Yuan
authors: XY
authors: Liu
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D017209:Apoptosis; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D017931:DNA Primers; D015316:Genetic Therapy; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050130:Oncolytic Virotherapy; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000473:pathology / Q000628:therapy*; D000099180:Interleukin-24
chemical_list: D000951:Antigens, Neoplasm; D017931:DNA Primers; D007378:Interleukins; D000099180:Interleukin-24; C479341:prostate cancer antigen 3, human