Viability and growth characteristics of Lactobacillus in soymilk supplemented with B-vitamins.
Ten strains of Lactobacillus were evaluated for their viability in soymilk. Lactobacillus acidophilus ATCC 314, L. acidophilus FTDC 8833, L. acidophilus FTDC 8633 and L. gasseri FTDC 8131 displayed higher viability in soymilk and were thus selected to be evaluated for viability and growth characteristics in soymilk supplemented with B-vitamins. Pour plate analyses showed that the supplementation of all B-vitamins studied promoted the growth of lactobacilli to a viable count exceeding 7 log CFU/ml. alpha-Galactosidase specific activity of lactobacilli as determined spectrophotometrically showed an increase upon supplementation of B-vitamins. High-performance liquid chromatography analyses revealed that this led to increased hydrolysis of soy oligosaccharides and subsequently higher utilization of simple sugars. Production of organic acids as determined via high-performance liquid chromatography also showed an increase, accompanied by a decrease in pH of soymilk. Additionally, the supplementation of B-vitamins also promoted the synthesis of riboflavin and folic acid by lactobacilli in soymilk. Our results indicated that B-vitamin-supplemented soymilk is a good proliferation medium for strains of lactobacilli.
Ewe
Joo-Ann
JA
Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Malaysia.
Wan-Abdullah
Wan-Nadiah
WN
Liong
Min-Tze
MT
D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D019587:Dietary Supplements; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007778:Lactobacillus / Q000254:growth & development*; D007779:Lactobacillus acidophilus / Q000254:growth & development*; D050296:Microbial Viability; D009844:Oligosaccharides / Q000378:metabolism; D019936:Probiotics; D041942:Soy Milk / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max; D014803:Vitamin B Complex / Q000096:biosynthesis / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000378:metabolism
D009844:Oligosaccharides; D014803:Vitamin B Complex; D000519:alpha-Galactosidase