title: Sulfated hyaluronan derivatives reduce the proliferation rate of primary rat calvarial osteoblasts.
abstract: Glycosaminoglycans (GAG) and proteoglycans, which are components of the extracellular bone matrix, are also localized in and at the membrane of osteoblasts and in the pericellular matrix. Due to their interaction with several growth factors, water and cations these molecules play an important role in regulating proliferation and differentiation of osteoblasts and bone development. The aim of this study was to assess in vitro the effects of two chemically sulfated hyaluronan (HyaS) derivatives on the proliferation of rat calvarial osteoblasts and to compare with those of native hyaluronan (Hya) and natural sulfated GAG such as chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), dermatan sulfate (DS) and heparan sulfate (HS). Moderately and highly sulfated HyaS derivatives caused a time-dependent reduction of osteoblast proliferation. The anti-proliferative effect of HyaS was accompanied by a cell cycle arrest in the G1 phase, but was not associated with cell death. Whereas non-sulfated high molecular weight (HMW)- and low molecular weight (LMW)-Hya as well as C4S, C6S, DS and HS showed no effect on the cell proliferation.
authors: Institute of Physiological Chemistry, Dresden University of Technology, Fiedlerstrasse 42, 01307, Dresden, Germany. reiner.kunze@mailbox.tu-dresden.de
authors: Reiner
authors: R
authors: Kunze
authors: Manuela
authors: M
authors: Rösler
authors: Stephanie
authors: S
authors: Möller
authors: Matthias
authors: M
authors: Schnabelrauch
authors: Thomas
authors: T
authors: Riemer
authors: Ute
authors: U
authors: Hempel
authors: Peter
authors: P
authors: Dieter
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000166:cytology*
chemical_list: D006820:Hyaluronic Acid; D004247:DNA; D007770:L-Lactate Dehydrogenase