title: Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
abstract: Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
authors: Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors: Alon
authors: A
authors: Goren
authors: Fatih
authors: F
authors: Ozsolak
authors: Noam
authors: N
authors: Shoresh
authors: Manching
authors: M
authors: Ku
authors: Mazhar
authors: M
authors: Adli
authors: Chris
authors: C
authors: Hart
authors: Melissa
authors: M
authors: Gymrek
authors: Or
authors: O
authors: Zuk
authors: Aviv
authors: A
authors: Regev
authors: Patrice M
authors: PM
authors: Milos
authors: Bradley E
authors: BE
authors: Bernstein
mesh_terms: D000818:Animals; D015982:Bias; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics; D006801:Humans; D051379:Mice; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; D014157:Transcription Factors