Functional and multifunctional nanoparticles for bioimaging and biosensing.
Herein, we describe the synthesis of functional and multifunctional nanoparticles (NPs), derived from our recent work, for bioimaging and biosensing applications. The functionalized NPs involve quantum dots (QDs), magnetic particles (MPs) and noble metal NPs for the aforementioned applications. A diverse silica coating approaches (reverse microemulsion and thin silanization) are delineated for the design of water-soluble NPs. We also review the synthesis of silica-coated bifunctional NPs consisting of MPs and QDs for live cell imaging of human liver cancer cells (HepG2) and mouse fibroblast cells (NIH-3T3). Using silica coated NPs, various NPs that are functionalized with antibody, oligonucleotide, biotin and dextran are efficiently used for protein detection.
Selvan
Subramanian Tamil
ST
Institute of Materials Research and Engineering, 3 Research Link, Singapore 117602. subramaniant@imre.a-star.edu.sg
Tan
Timothy Thatt Yang
TT
Yi
Dong Kee
DK
Jana
Nikhil R
NR
D000818:Animals; D035843:Biomedical Research; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
D012822:Silicon Dioxide