Na+/H+ exchanger blockade inhibits the expression of vascular endothelial growth factor in SGC7901 cells.
Vascular endothelial growth factor (VEGF) overexpression is critically involved in tumor formation. Na+/H+ exchanger isoform (NHE) is elevated in cancer cells. We explored the effect of NHE inhibition on VEGF expression in human gastric cancer SGC7901 cells. VEGF mRNA expression was detected by real-time RT-PCR. VEGF protein expression was measured by western blotting and immunocytochemistry. For determination of viable cells the MTT colorimetric assay was used. Treatment of SGC7901 cells with NHE inhibitors resulted in significant decrease of VEGF mRNA and protein expression. NHE inhibition decreased intracellular pH (pHi) values and VEGF mRNA expression. These data demonstrate that NHE blockade inhibits VEGF expression in SGC7901 cells.
He
Bingyan
B
Department of Pediatrics, Zhongnan Hospital of Wuhan University, Hubei Province, P.R. China. heby2005@sina.com
Zhang
Ming
M
Zhu
Rui
R
D049109:Cell Proliferation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry / Q000379:methods; D020014:K562 Cells; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000378:metabolism
D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers; D013778:Tetrazolium Salts; D013844:Thiazoles; D042461:Vascular Endothelial Growth Factor A; C022616:thiazolyl blue