Quantitative measurements of absorption spectra in scattering media by low-coherence spectroscopy.
Low-coherence spectroscopy (LCS) is a spectroscopic method that allows for quantitative and localized assessment of absorption spectra by combining reflection spectroscopy with low-coherence interferometry. We describe absorption coefficient (mu(a)) measurements by LCS in tissue simulating phantoms with varying scattering and absorbing properties. We used LCS in the 455-680 nm wavelength range with a spectral resolution of 8 nm to obtain mu(a) spectra with +/-0.5 mm(-1) accuracy. We conclude that LCS is a promising technique for the in vivo determination of tissue chromophore concentrations.
Bosschaart
Nienke
N
Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700,NL-1100 DE Amsterdam, The Netherlands. n.bosschaart@amc.uva.nl
Aalders
Maurice C G
MC
Faber
Dirk J
DJ
Weda
Jelmer J A
JJ
van Gemert
Martin J C
MJ
van Leeuwen
Ton G
TG
D000042:Absorption; D007368:Interferometry; D019047:Phantoms, Imaging; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*
D011137:Polystyrenes