[Particularities of alkane oxidation in Rhodococcus erythropolis EK-1 strain--producer of surface-active substances].
The hexadecane oxidation of Rhodococcus erythropolls EK-1 was established to be catalyzed by the alkane hydroxylase complex containing Fe-S proteid rubredoxin. Alkane hydroxylase activity was increased twice in the presence of 10 mg/l iron ions in the cultivation medium. Sodium ions were shown to be an activator of alkane hydroxylase. Four types of alcohol dehydrogenases (NAD, NADP, pyrroloquinoline quinone and N,N-dimethyll-4-nitrosoaniline-dependent enzymes) were found in hexadecane-grown cells of R. erythropolis EK-1. The obtained data are the basis for the improvement of surface active substances technology.
Pyroh
T P
TP
Shevchuk
T A
TA
Klymenko
Iu O
IuO
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000473:Alkanes / Q000737:chemistry*; D003470:Culture Media; D042926:Cytochrome P-450 CYP4A / Q000037:antagonists & inhibitors / Q000378:metabolism; D005233:Fatty Alcohols / Q000737:chemistry; D007501:Iron / Q000494:pharmacology; D010084:Oxidation-Reduction; D011188:Potassium / Q000494:pharmacology; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology; D013501:Surface-Active Agents / Q000302:isolation & purification* / Q000378:metabolism
D000473:Alkanes; D003470:Culture Media; D005233:Fatty Alcohols; D013501:Surface-Active Agents; C005031:cetyl alcohol; D012964:Sodium; D007501:Iron; D000426:Alcohol Dehydrogenase; D042926:Cytochrome P-450 CYP4A; C007932:n-hexadecane; D011188:Potassium