Isolation of antagonists of antigen-specific autoimmune T cell proliferation.
Antigen-specific T cells play a major role in mediating the pathogenesis of a variety of autoimmune conditions as well as other diseases. In the context of experimental autoimmune encephalomyelitis, a murine model of multiple sclerosis, we present here a general approach to the discovery of highly specific ligands for autoreactive cells. These ligands are obtained from a combinatorial library of hundreds of thousands of synthetic peptoids that is screened simultaneously against two populations of CD4+ T cells. Peptoids that recognize autoreactive T cells with extremely high specificity can be identified in the library. Since no specific knowledge is required regarding the nature of the native antigens recognized by the autoreactive T cells, this technology provides a powerful tool for the enrichment and inhibition of autoimmune cells in a variety of disease states.
Gocke
Anne R
AR
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, 75390-9185, USA.
Udugamasooriya
D Gomika
DG
Archer
Chase T
CT
Lee
Jiyong
J
Kodadek
Thomas
T
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D020650:Combinatorial Chemistry Techniques; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D019151:Peptide Library; D034444:Peptoids / Q000737:chemistry / Q000494:pharmacology*
D004676:Myelin Basic Protein; D010446:Peptide Fragments; D019151:Peptide Library; D034444:Peptoids; C405722:myelin basic protein 1-11