[The comparison of CD4(+);CD25(high); Foxp3(+); regulatory T cells between neonatal cord blood and adult peripheral blood.].
OBJECTIVE
To quantify the proportion of CD4(+);CD25(high); Foxp3(+); regulatory T cells (Treg) in neonatal cord blood and adult peripheral blood, and to explore the clinical significance of Treg in neonatal cord blood.


METHODS
The percentage of CD4(+); T cells, and ratio of CD4(+);CD25(+);/CD4(+); and CD4(+);CD25(high);/CD4(+);T cells in mononuclear cells in 30 neonatal cord blood and 27 adult peripheral blood were examinea with flow cytometry (FCM). The expression of Foxp3(+); in CD4(+);CD25(+); and CD4(+);CD25(high); T cells was examined with FCM and RT-PCR, respectively RT-PCR.


RESULTS
Compared with adult PBMC, the percentage of CD4(+); T cells was increased in neonatal cord blood(P<0.01); the percentage of CD4(+);CD25(+);/CD4(+); and CD4(+);CD25(high);/CD4(+);T cells were decreased in neonatal cord blood(P<0.05). The percentage of Foxp3(+); cells in CD4(+);CD25(+); and CD4(+);CD25(high); T cells in neonatal cord blood were both lower than that in adult peripheral blood(P<0.01) and the expression level of Foxp3 mRNA was also lower than that in adult peripheral blood(P<0.05).


CONCLUSIONS
There are certain amount CD4(+);CD25(high); Tregs in neonatal cord blood, but the expression levels of Foxp3 are lower than that in adult peripheral blood, which indicate that Tregs might play a distinct role of immunoloregulation.
Wang
Xue-Ye
XY
Department of Hematology and Rheumatics, Jilin Provincial Hospital, Changchun 130021, China.
Han
Mei
M
Xiao
Zhong-Ping
ZP
Chen
Lin
L
Kong
Ling-Huan
LH
Fu
Kun
K
Feng
Tao
T
Li
Ling-Jun
LJ
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005312:Fetal Blood / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D051858:Forkhead Transcription Factors