title: [Regulatory effects of Wuzhuyutang (Evodiae prescription) and its consisting herbs on TPH2 promoter].
abstract: OBJECTIVE
  To screen the active component of Wuzhuyutang (WZYT, Evodiae prescription) and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine.
  
  
  METHODS
  By transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated.
  
  
  RESULTS
  Different regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed.
  
  
  CONCLUSIONS
  TPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.
authors: Laboratory of Pharmaceutics, Department of Biological Sciences and Biotechnology, Tsignghua University, Beijng 100084, China.
authors: Yugang
authors: Y
authors: Wang
authors: Fan
authors: F
authors: Lei
authors: Xiukun
authors: X
authors: Wang
authors: Jun
authors: J
authors: Hu
authors: Honglei
authors: H
authors: Zhan
authors: Dongming
authors: D
authors: Xing
authors: Lijun
authors: L
authors: Du
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D032086:Evodia / Q000737:chemistry*; D006801:Humans; D008881:Migraine Disorders / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D016716:PC12 Cells; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D014365:Tryptophan Hydroxylase / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D014365:Tryptophan Hydroxylase; C504569:tph2 protein, rat