The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy.
Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy.
Lighezan
Rodica
R
Department of Histology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania. rodalighezan@yahoo.com
Baderca
Flavia
F
Alexa
Aurora
A
Iacovliev
M
M
Bonţe
Diana
D
Murărescu
Elena Doina
ED
Nebunu
Adriana
A
D005557:Formaldehyde / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008853:Microscopy; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D009993:Osmium Tetroxide / Q000737:chemistry; D016612:Paraffin Embedding / Q000379:methods*; D016707:Tissue Fixation; D014571:Urologic Neoplasms / Q000648:ultrastructure*
D005557:Formaldehyde; D009993:Osmium Tetroxide; D005976:Glutaral