title: Identification of the Mesorhizobium loti gene responsible for glycerophosphorylation of periplasmic cyclic beta-1,2-glucans.
abstract: Periplasmic cyclic beta-1,2-glucans play a crucial role in symbiosis as well as in hypo-osmotic adaptation for rhizobia. These glucans are modified in many species by anionic substituents such as glycerophosphoryl and succinyl ones, but their role remains to be examined. In this work, the cgmA homolog is shown to be responsible for glycerophosphorylation of cyclic beta-1,2-glucans in Mesorhizobium loti. The mutation in cgmA converted most anionic glucans into neutral ones, leaving a small amount of succinylated ones. An additional mutation in opgC, which encodes a succinyltransferase homolog, abolished the residual succinyl substituents in the cgmA-mutant background. The double mutant in cgmA and opgC did not show any significant phenotypic differences from the wild type during both vegetative growth and symbiosis. It is concluded that the anionic substituents make a minor contribution, if any, to the effectiveness of cyclic beta-1,2-glucans in M. loti.
authors: Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, Japan.
authors: Yasuyuki
authors: Y
authors: Kawaharada
authors: Hiromasa
authors: H
authors: Kiyota
authors: Shima
authors: S
authors: Eda
authors: Kiwamu
authors: K
authors: Minamisawa
authors: Hisayuki
authors: H
authors: Mitsui
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005994:Glycerophosphates / Q000378:metabolism*; D014166:Transferases / Q000235:genetics / Q000378:metabolism*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005994:Glycerophosphates; D047071:beta-Glucans; C033744:beta-1,2-glucan; D014166:Transferases