title: The brome mosaic virus-based recombination vector triggers a limited gene silencing response depending on the orientation of the inserted sequence.
abstract: In some RNA viruses (e.g. in brome mosaic virus, BMV), the same factor (intra- or intermolecular hybridization between viral RNA molecules) is capable of inducing two different processes: RNA silencing and RNA recombination. To determine whether there is some interplay between these two phenomena, we have examined if the BMV-based recombination vector containing a plant-genome-derived sequence can function as a gene-silencing vector. Surprisingly, we found that neither dsRNA forming during the replication of the BMV-based vector nor highly structured regions of its genome were effective RNAi triggers. Only mutants carrying a sequence complementary to the target mRNA functioned as gene silencing vectors and were steadily maintained in the infected plant. The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells.
authors: Department of Gene Expression, Adam Mickiewicz University, Miedzychodzka 5, 60-371, Poznan, Poland.
authors: Andrzej
authors: A
authors: Pacak
authors: Pawel M
authors: PM
authors: Strozycki
authors: Maria
authors: M
authors: Barciszewska-Pacak
authors: Magdalena
authors: M
authors: Alejska
authors: Christophe
authors: C
authors: Lacomme
authors: Artur
authors: A
authors: Jarmołowski
authors: Zofia
authors: Z
authors: Szweykowska-Kulińska
authors: Marek
authors: M
authors: Figlerowicz
mesh_terms: D017795:Bromovirus / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods*; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D014026:Nicotiana / Q000235:genetics
chemical_list: D012367:RNA, Viral