The effect of gentian violet on virulent properties of Candida albicans.
The aim of this study was to evaluate the effect of gentian violet (GV) on phospholipase activity, proteinase activity and germ tube formation rate of Candida albicans. Both 12 phospholipase-positive and 12 proteinase-positive C. albicans isolates with Pz values <or=0.89 were obtained. A yeast suspension (1-3 x 10(7) cfu/ml) of each isolate was prepared. After a brief exposure (60 min) to sub-therapeutic concentrations (0.5 or 2 microg/ml) of GV, Pz value of phospholipase, Pz value of proteinase and germ tube formation rate were determined. Phospholipase activity, proteinase activity and germ tube formation rate in two groups exposed to GV were significantly lower than those in the group unexposed (P < 0.05). The results of this study indicated that sub-therapeutic concentrations of GV may lead to reduction in phospholipase activity, proteinase activity and germ tube formation, and then may suppress virulence and pathogenicity of C. albicans.
Ying
Su
S
Department of Dermatology and Ethology, Qilu Hospital Affiliated to Shandong University, Jinan, China. sywjzwxl@yahoo.com.cn
Qing
Sun
S
Chunyang
Li
L
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology / Q000472:pathogenicity*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D005840:Gentian Violet / Q000494:pharmacology*; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D010740:Phospholipases / Q000037:antagonists & inhibitors; D014774:Virulence / Q000187:drug effects
D000935:Antifungal Agents; D005656:Fungal Proteins; D010740:Phospholipases; D010447:Peptide Hydrolases; D005840:Gentian Violet