title: No association of the insulin gene VNTR polymorphism with polycystic ovary syndrome in a Han Chinese population.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with an increased risk of type II diabetes mellitus. The results of previous research about the association of the VNTR polymorphism in 5-prime flanking region of the insulin (INS) gene with PCOS have been inconsistent. The present study was to investigate the association of the INS-VNTR polymorphism with PCOS in a Han Chinese population.
  
  
  METHODS
  The -23/HphI polymorphism as a surrogate marker of the INS-VNTR length polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) in 216 PCOS patients and 192 non-PCOS women as a control group. Allelic and genotypic frequencies were compared between patients and controls, and these results were analyzed in respect to clinical test data.
  
  
  RESULTS
  No significant differences were observed between the cases and controls groups either in allele (P = 0.996) or genotype (P = 0.802) frequencies of INS-VNTR polymorphism; Regarding anthropometric data and hormone levels, there were no significant differences between INS-VNTR genotypes in the PCOS group, as well as in the non-PCOS group.
  
  
  CONCLUSIONS
  The present study demonstrated for the first time that the INS-VNTR polymorphism is not a key risk factor for sporadic PCOS in the Han Chinese women. Further studies are needed to give a global view of this polymorphism in pathogenesis of PCOS in a large-scale sample, family-based association design or well-defined subgroups of PCOS.
authors: Department of Obstetrics & Gynecology, the First Affiliated Hospital, Anhui Medical University, Hefei 230022, China. allin0701@gmail.com
authors: Yuping
authors: Y
authors: Xu
authors: Zhaolian
authors: Z
authors: Wei
authors: Zhiguo
authors: Z
authors: Zhang
authors: Qiong
authors: Q
authors: Xing
authors: Pin
authors: P
authors: Hu
authors: Xiaohui
authors: X
authors: Zhang
authors: Guihua
authors: G
authors: Gao
authors: Yong
authors: Y
authors: Wang
authors: Qian
authors: Q
authors: Gao
authors: Long
authors: L
authors: Yi
authors: Yunxia
authors: Y
authors: Cao
mesh_terms: D000328:Adult; D000483:Alleles; D002681:China / Q000453:epidemiology; D004247:DNA / Q000235:genetics; D005006:Ethnicity; D005260:Female; D005838:Genotype; D012739:Gonadal Steroid Hormones / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012739:Gonadal Steroid Hormones; D007328:Insulin; D004247:DNA