title: [Study on polymorphism of UCP2 gene in Chengdu simple obesity and normal-weight people and a preliminary investigation of its relationship with gut bacteria].
abstract: OBJECTIVE
  To study on the polymorphism of UCP2 gene in Chengdu simple obesity and normal-weight people and to initially investigate the relationship between UCP2 Ala55Val variation and gut bacteria.
  
  
  METHODS
  PCR-PFLP was applied to determine the genotypes of Ala55Val variant in the UCP2 gene of 86 Chengdu people (the simple obesity group, 43 subjects; the normal-weight group, 43 subjects). And six kinds of gut bacteria among different genotypes in different groups were analyzed.
  
  
  RESULTS
  Both the simple obesity and the normal-weight group had the Ala55Val variants of Ala/Ala, Val/Val and Ala/Val in the UCP2 gene, and the Ala55Val genotype distributions between the two groups was significantly different (chi2=11.97, P< 0.05). The allelic mutation frequency in the simple obesity group was higher than that of the normal-weight group (chi2=10.06, P<0.05). However, no significant difference was observed in the population of six gut bacteria among different genotypes in different groups (P>0.05).
  
  
  CONCLUSIONS
  The UCP2 gene mutation might be a risk factor of obesity in Chengdu area. However, this gene mutation may not be an impact factor on the alternation of gut bacteria.
authors: Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Hao-Jiang
authors: HJ
authors: Zuo
authors: Wei-Wei
authors: WW
authors: Zhang
authors: Zhi-Mei
authors: ZM
authors: Xie
authors: Yong-Ru
authors: YR
authors: Li
authors: Wei
authors: W
authors: Wang
authors: Xiao-Bei
authors: XB
authors: Ding
authors: Xiao-Fang
authors: XF
authors: Pei
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D002681:China; D005260:Female; D005838:Genotype; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D009154:Mutation; D009765:Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors; D000071246:Uncoupling Protein 2; D055815:Young Adult
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2