Extract of the aerial parts of Aster koraiensis reduced development of diabetic nephropathy via anti-apoptosis of podocytes in streptozotocin-induced diabetic rats.
Advanced glycation end products (AGEs) is produced from glycolysis in vivo, which may result in diabetic nephropathy. Podocyte loss has been implicated in the development of diabetic nephropathy. The aim of this study was to investigate the protective effects of Aster koraiensis extract (AKE), on the damage of renal podocytes in streptozotocin (STZ)-induced diabetic rats. AKE (100, 200mg/kg per day) was given to diabetic rats for 13weeks. Blood glucose, glycated haemoglobin (HbA1c), proteinuria and albuminuria were examined. Kidney histopathology, AGEs accumulation, apoptosis, and expression of Bax and Bcl-2 also were examined. In 20-week-old STZ-induced diabetic rats, severe hyperglycemia was developed, and proteinuria and albuminuria were markedly increased. TUNEL-positive signals were highly detected in glomeruli of STZ-induced diabetic rats. However, AKE reduced proteinuria and albuminuria in diabetic rats. AKE prevented AGEs deposition and podocyte apoptosis. Expression of Bax and Bcl-2 protein were restored by AKE treatment in the renal cortex. These results suggested that AKE has an inhibitory effect of AGE accumulation and anti-apoptotic effect in the glomeruli of diabetic rat. AKE could be beneficial in preventing the progression of diabetic nephropathy.
Sohn
Eunjin
E
Diabetic Complications Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, South Korea.
Kim
Junghyun
J
Kim
Chan-Sik
CS
Kim
Young Sook
YS
Jang
Dae Sik
DS
Kim
Jin Sook
JS
D000818:Animals; D031173:Aster Plant / Q000737:chemistry*; D019610:Cytoprotection; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D017127:Glycation End Products, Advanced / Q000097:blood / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000627:therapeutic use*; D050199:Podocytes / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D013311:Streptozocin