title: Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages.
abstract: The aim of our study was to analyse chromosomal aneuploidy occurrence in rabbit oocytes and embryos. Chromosomal analysis was done in rabbit oocytes and rabbit preimplantation embryos at 2-, 4- and 8-cell stages derived from in vivo fertilization. For mitotic cycle synchronization at the metaphase stage, 2-, 4- and 8-cell embryos were incubated in k-DMEM medium, supplemented with colcemid (1 microg/ml), for 7, 12 or 13h respectively. Success of metaphase synchronization was at values of 100, 86.1 and 92.2% for 2-, 4- and 8-cell embryos respectively. Recovery rate of analysable metaphase plates was reached at 58.8%, 83.9% and 59.8% for 2-, 4- and 8-cell embryos and 100% for oocytes. Significant difference (p < 0.01) in aneuploidy rate between oocytes (40.7%) and 2-cell embryos (62.5%) was found. These results demonstrate higher efficiency of synchronization of embryo cells at the metaphase stage, what may contribute to elevating the proportion of analysable nuclei.
authors: Department of Genetics and Animal Reproduction, Animal Production Research Centre, Nitra, Luzianky, Slovak Republic. jojonr@zoznam.sk
authors: Jozef
authors: J
authors: Curlej
authors: Jozef
authors: J
authors: Bulla
authors: Peter
authors: P
authors: Chrenek
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002875:Chromosomes; D004622:Embryo, Mammalian; D047108:Embryonic Development; D008677:Metaphase; D008938:Mitosis; D009865:Oocytes; D011817:Rabbits