Molecular cloning and expression analysis of bat toll-like receptors 3, 7 and 9.
In this study, cDNA of Toll-like receptors (TLR) 3, 7 and 9 were synthesized and completely sequenced. The coding regions of cDNA for bat TLR3, TLR7 and TLR9 were 2,718, 3,150 and 3,090 bp in length, respectively. The open reading frames encoded 905, 1,049 and 1,029 amino acids for TLR3, TLR7 and TLR9, respectively. The nucleotide sequences, predicted amino acid sequences and predicted domain structures of the three bat TLRs had high homology with those of other mammals. In addition, the expression profiles of each TLR in main organs were analyzed. Expression of TLR3 was highest in the liver, whereas the expressions of TLR7 and TLR9 were highest in the spleen.
Iha
Koichiro
K
Department of Biomedical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa097127@mail.ecc.u-tokyo.ac.jp
Omatsu
Tsutomu
T
Watanabe
Shumpei
S
Ueda
Naoya
N
Taniguchi
Satoshi
S
Fujii
Hikaru
H
Ishii
Yoshiyuki
Y
Kyuwa
Shigeru
S
Akashi
Hiroomi
H
Yoshikawa
Yasuhiro
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000276:immunology*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D051199:Toll-Like Receptor 7 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D051193:Toll-Like Receptors / Q000235:genetics*
D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors; D012313:RNA