Metabolic enzyme(s) confer imidacloprid resistance in a clone of Myzus persicae (Sulzer) (Hemiptera: Aphididae) from Greece.
BACKGROUND
Previous studies have reported varying levels of resistance against imidacloprid in several insect species, including populations of the peach-potato aphid, Myzus persicae (Sulzer). These cases of resistance have been attributed to either target-site resistance or enhanced detoxification. In this study, a clone of M. persicae originating from Greece revealed a 60-fold resistance factor to imidacloprid.


RESULTS
The Greek clone is compared in terms of metabolic enzyme activity and synergism profiles with other M. persicae clones showing lower imidacloprid resistance.


CONCLUSIONS
A combination of in vitro biochemical assays and in vivo differential synergism studies using PBO and a close analogue EN 16/5-1 suggests that the mechanism conferring increased resistance in this clone is primarily due to enhanced oxidase activity.
Philippou
Despina
D
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK.
Field
Linda
L
Moores
Graham
G
D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001681:Biological Assay; D019976:Cloning, Organism; D016002:Discriminant Analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D006115:Greece; D007093:Imidazoles / Q000494:pharmacology*; D007305:Insecticide Resistance / Q000187:drug effects* / Q000235:genetics; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000494:pharmacology*; D010882:Piperonyl Butoxide
D007093:Imidazoles; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D010882:Piperonyl Butoxide