title: Potency of double-layered poly L-lactic acid scaffold in tissue engineering of tendon tissue.
abstract: A successful scaffold for use in tendon tissue engineering requires a high affinity for living organisms and the ability to maintain its mechanical strength until maturation of the regenerated tissue. We compared two types of poly(L-lactic acid) (PLLA) scaffolds for use in tendon regeneration, a plain-woven PLLA fabric (fabric P) with a smooth surface only and a double layered PLLA fabric (fabric D) with a smooth surface on one side and a rough (pile-finished) surface on the other side. These two types of fabric were implanted into the back muscles of rabbits and evaluated at three and six weeks after implantation. Histological examination showed collagen tissues were highly regenerated on the rough surface of fabric D. On the other hand, liner cell attachment was seen in the smooth surface of fabric P and fabric D. The total DNA amount was significantly higher in fabric D. Additionally, mechanical examination showed fabric P had lost its mechanical strength by six weeks after implantation, while the strength of fabric D was maintained. Fabric D had more cell migration on one side and less cell adhesion on the other side and maintained its initial strength. Thus, a novel form of double-layered PLLA fabric has the potential to be used as a scaffold in tendon regeneration.
authors: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Chuo-ku, Japan. atsuyukii@yahoo.co.jp
authors: Atsuyuki
authors: A
authors: Inui
authors: Takeshi
authors: T
authors: Kokubu
authors: Takeshi
authors: T
authors: Makino
authors: Issei
authors: I
authors: Nagura
authors: Narikazu
authors: N
authors: Toyokawa
authors: Ryosuke
authors: R
authors: Sakata
authors: Masaru
authors: M
authors: Kotera
authors: Takashi
authors: T
authors: Nishino
authors: Hiroyuki
authors: H
authors: Fujioka
authors: Masahiro
authors: M
authors: Kurosaka
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013314:Stress, Mechanical; D013710:Tendons / Q000378:metabolism / Q000637:transplantation*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen