beta-1,3-glucanase inhibits activity of the killer toxin produced by the marine-derived yeast Williopsis saturnus WC91-2.
The marine-derived Williopsis saturnus WC91-2 was found to produce very high killer toxin activity against the pathogenic yeast Metschnikowia bicuspidata WCY isolated from the diseased crab. It is interesting to observe that the purified beta-1,3-glucanase from W. saturnus WC91-2 had no killer toxin activity but could inhibit activity of the WC91-2 toxin produced by the same yeast. In contrast, the WC91-2 toxin produced had no beta-1,3-glucanase activity. We found that the mechanisms of the inhibition may be that the beta-1,3-glucanase competed for binding to beta-1,3-glucan on the sensitive yeast cell wall with the WC91-2 toxin, causing decrease in the amount of the WC91-2 toxin bound to beta-1,3-glucan on the sensitive yeast cell wall and the activity of the WC91-2 toxin against the sensitive yeast cells. In order to make W. saturnus WC91-2 produce high activity of the WC91-2 toxin against the yeast disease in crab, it is necessary to delete the gene encoding beta-1,3-glucanase.
Peng
Ying
Y
Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No.5, Qingdao, China.
Chi
Zhenming
Z
Wang
Xianghong
X
Li
Jing
J
D043326:Glucan 1,3-beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*; D005936:Glucans; D008387:Marine Toxins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity; D009183:Mycotoxins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity; D011134:Polysaccharides / Q000378:metabolism; D011523:Protoplasts / Q000187:drug effects; D055320:Williopsis / Q000737:chemistry* / Q000201:enzymology* / Q000648:ultrastructure
D005936:Glucans; D008387:Marine Toxins; D009183:Mycotoxins; D011134:Polysaccharides; C008247:laminaran; D043326:Glucan 1,3-beta-Glucosidase