title: Minimum length of homology arms required for effective Red/ET recombination.
abstract: The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.
authors: Graduate School of Innovative Life Science, University of Toyama, Japan.
authors: Rika
authors: R
authors: Fujimoto
authors: Toshihiko
authors: T
authors: Osakabe
authors: Masumichi
authors: M
authors: Saito
authors: Nobuyuki
authors: N
authors: Kurosawa
authors: Masaharu
authors: M
authors: Isobe
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000191:economics / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid