title: Hepatic histopathological characteristics and antioxidant response of phytoplanktivorous silver carp intraperitoneally injected with extracted microcystins.
abstract: OBJECTIVE
  To investigate the hispathological characteristics and antioxidant responses in liver of silver carp after intraperitoneal administration of microcystins (MCs) for further understanding hepatic intoxication and antioxidation mechanism in fish.
  
  
  METHODS
  Phytoplanktivorous silver carp was injected intraperitoneally (i.p.) with extracted hepatotoxic microcystins (mainly MC-RR and -LR) at a dose of 1000 microg MC-LReq./kg body weight, and liver histopathological changes and antioxidant responses were studied at 1, 3, 12, 24, and 48 h, respectively, after injection.
  
  
  RESULTS
  The damage to liver structure and the activities of hepatic antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxide (GPX) were increased in a time-dependent manner.
  
  
  CONCLUSIONS
  In terms of clinical and histological signs of intoxication and LD50 (i.p.) dose of MC-LR, silver carp appears rather resistant to MCs exposure than other fishes. Also, the significantly increased SOD activity in the liver of silver carp suggests a higher degree of response to MCs exposure than CAT and GPX.
authors: Fisheries College of Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors: Li
authors: L
authors: Li
authors: Ping
authors: P
authors: Xie
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D052998:Microcystins / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology*; D010839:Phytoplankton / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D000975:Antioxidants; D052998:Microcystins; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase