In vivo imaging of obesity-induced inflammation in adipose tissue.
Obesity is associated with low-grade inflammation in adipose tissue, which contributes to the development of obesity-related diseases such as insulin resistance, hypertension and arteriosclerosis. Here we developed an animal model that non-invasively monitors inflammation in adipose tissue using in vivo bioluminescent imaging (BLI) technique. In vitro, stimulation with TNFalpha or co-culture with RAW264 macrophages increased bioluminescence in 3T3-L1 adipocytes expressing NF-kappaB-mediated luciferase gene (3T3-L1/NF-kappaB-re-luc2P). In vivo, lipopolysaccharide increased bioluminescence in mice transplanted with 3T3-L1/NF-kappaB-re-luc2P cells. Moreover, light emission derived from implanted cells was significantly higher in diet-induced obese mice transplanted with 3T3-L1/NF-kappaB-re-luc2P than in lean mice. Our results showed that BLI technique and 3T3-L1/NF-kappaB-re-luc2P cells provide a useful approach to non-invasively monitor obesity-induced inflammation in adipose tissue in in vivo.
Fushiki
Hiroshi
H
Pharmacology, Tsukuba Research Institute, Banyu Pharmaceutical Co, Ltd, 3 Okubo, Tsukuba, Ibaraki 300-2611, Japan. fushiki@mprcf.or.jp
Hayakawa
Yoshihiro
Y
Gomori
Akira
A
Seo
Toru
T
Tewari
Shruti
S
Ozaki
Satoshi
S
Yoshimoto
Ryo
R
D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D017930:Genes, Reporter; D007249:Inflammation / Q000209:etiology / Q000473:pathology*; D008070:Lipopolysaccharides; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008991:Monitoring, Physiologic; D016328:NF-kappa B / Q000378:metabolism; D009765:Obesity / Q000473:pathology*
D008070:Lipopolysaccharides; D016328:NF-kappa B; D008156:Luciferases