[Comparative enantiomer separation of beta-blockers on polysaccharide derived chiral stationary phases using high performance liquid chromatography with acid or base additive in the mobile phases].
The liquid chromatographic enantiomer separation of beta-blockers on several polysaccharide derived chiral stationary phases (CSPs) (Chiralpak AD, Chiralcel OD, Chiralpak IA and Chiralpak IB) was performed and compared in the normal phase mode using hexane-ethanol in the presence of acid or base additives. The chromatographic conditions were 10% - 30% (v/v) ethanol-hexane containing 0.1% trifluoroacetic acid or triethylamine as the mobile phase at the flow rate of 1.0 mL/min with the detection at 254 nm. The lower enantioselectivities and the shorter retention times on amylose derived CSPs (Chiralpak AD and Chiralpak IA) using the mobile phase with acid additive than those with base additive were shown, except for slightly longer retention times of metoprolol and propranolol on Chiralpak AD. The greater enantioselectivities and the shorter retention times on cellulose derived CSPs (Chiralcel OD and Chiralpak IB) using the mobile phase with acid additive than those with base additive were shown, especially, Chiralcel OD showed dramatically enhanced enantioselectivites using the mobile phase with acidic additive. Also, it was shown that the greater enantiomer separation of beta-blockers on Chiralcel OD was achieved using the mobile phases with the higher concentration acid additive.
Huang
Hu
H
College of Pharmacy, Chosun University, Gwangju, Republic of Korea.
Jin
Jingyu
J
Lee
Wonjae
W
D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism; D014238:Trichloroacetic Acid / Q000737:chemistry
D000319:Adrenergic beta-Antagonists; D011134:Polysaccharides; D014238:Trichloroacetic Acid