Comparison of the AF146527 and B1 repeated elements, two real-time PCR targets used for detection of Toxoplasma gondii.
Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be the preferred diagnostic target.
Wahab
Tara
T
Centre for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden.
Edvinsson
Benjamin
B
Palm
Daniel
D
Lindh
Johan
J
D000818:Animals; D019411:Clinical Laboratory Techniques / Q000379:methods*; D016054:DNA, Protozoan / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology
D016054:DNA, Protozoan