Cell-free synthesis-based protein microarrays and their applications.
The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
Chandra
Harini
H
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
Srivastava
Sanjeeva
S
D000818:Animals; D002474:Cell-Free System; D004247:DNA; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics / Q000379:methods*
D011506:Proteins; D020543:Proteome; D004247:DNA