title: [The effect of murine mesenchymal stem cells on the differentiation and function of allogenetic bone marrow-derived dendritic cells.].
abstract: OBJECTIVE
  To study the effect of murine mesenchymal stem cells (MSCs) on the differentiation, maturation and function of allogenetic bone marrow-derived dendritic cells (DCs) and to investigate the mechanism of MSCs displaying immunoregulatory activity.
  
  
  METHODS
  BALB/c mice BMCs were isolated and cultured in vitro and then coclutured with C57BL/6 murine bone marrows cells (BMCs) at different ratios in vitro to induce DCs generation. The phenotype of cells was analyzed by flow cytometry. Endocytosis was measured as the cellular uptake of fluorescein isothiocyanate (FITC)-dextram amd was quantified by flow cytometry. The level of IL-12 secretion in the cell culture supernatants was detected by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Decreased expression of CD11c, CD14, CD83, CD86 and I-A(b);, down-regulated endocytosis capacity and interleukin-12 (IL-12) secretion of DCs were all observed when MSCs cocultured with BMCs at a higher ratio (1:10).
  
  
  CONCLUSIONS
  Murine MSCs could supress the differentiation and maturation of DCs derived from allogeneic BMCs in vitro.
authors: Clinical Medical Research Center, Shenzhen People's Hospital, The Second Clinic Medical College of Jinan University, Shenzhen 518020, China.
authors: Chun-Yan
authors: CY
authors: Deng
authors: Fu-Rong
authors: FR
authors: Li
authors: Xin-Gen
authors: XG
authors: Wang
authors: Hui
authors: H
authors: Qi
authors: Jin-Li
authors: JL
authors: Wen
authors: Ya-Ying
authors: YY
authors: Zhou
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL