title: A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome.
abstract: We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. As an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the stepwise chromosomal integration of the zeaxanthin biosynthesis pathway from Pantoea ananatis into E. coli.
authors: Institute of Microbiology, Universität Stuttgart, Stuttgart, Germany. christoph.albermann@imb.uni-stuttgart.de
authors: Christoph
authors: C
authors: Albermann
authors: Natalie
authors: N
authors: Trachtmann
authors: Georg A
authors: GA
authors: Sprenger
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D016254:Mutagenesis, Insertional / Q000235:genetics*; D020636:Pantoea / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism*; D024341:Xanthophylls / Q000235:genetics*; D065146:Zeaxanthins
chemical_list: D011994:Recombinant Proteins; D024341:Xanthophylls; D065146:Zeaxanthins