[CpG-oligodeoxynucleotide stimulation improves the success for karyotypic analysis of chronic lymphocytic leukemia cells].
OBJECTIVE
To explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome study of chronic lymphocytic leukemia (CLL).


METHODS
Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed.


RESULTS
The metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%.


CONCLUSIONS
CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.
Liu
Qiong
Q
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Xu
Wei
W
Qiu
Hai-rong
HR
Wang
Rong
R
Yu
Hui
H
Fan
Lei
L
Miao
Kou-rong
KR
Li
Jian-yong
JY
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009841:Oligonucleotides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C539572:CpG-ODN DSP30; D009841:Oligonucleotides