Differential ion mobility separations of peptides with resolving power exceeding 50.
Differential ion mobility spectrometry (IMS) or field asymmetric waveform IMS (FAIMS) separates gas-phase ions by mobility differences with respect to the electric field intensity. A major emerging FAIMS application is the fractionation of proteolytic digests. Using a planar FAIMS unit with helium/nitrogen mixtures, we have increased FAIMS resolving powers for peptide analyses from the prior maximum of approximately 20-30 to approximately 50-70. The resolution improved nearly 3-fold, allowing, in particular, separation of previously unresolved conformers.
Shvartsburg
Alexandre A
AA
Tang
Keqi
K
Smith
Richard D
RD
D001920:Bradykinin / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D013373:Substance P / Q000737:chemistry
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C063391:syntide-2; D013373:Substance P; D001920:Bradykinin