title: [The comparison of CD4(+);CD25(high); Foxp3(+); regulatory T cells between neonatal cord blood and adult peripheral blood.].
abstract: OBJECTIVE
  To quantify the proportion of CD4(+);CD25(high); Foxp3(+); regulatory T cells (Treg) in neonatal cord blood and adult peripheral blood, and to explore the clinical significance of Treg in neonatal cord blood.
  
  
  METHODS
  The percentage of CD4(+); T cells, and ratio of CD4(+);CD25(+);/CD4(+); and CD4(+);CD25(high);/CD4(+);T cells in mononuclear cells in 30 neonatal cord blood and 27 adult peripheral blood were examinea with flow cytometry (FCM). The expression of Foxp3(+); in CD4(+);CD25(+); and CD4(+);CD25(high); T cells was examined with FCM and RT-PCR, respectively RT-PCR.
  
  
  RESULTS
  Compared with adult PBMC, the percentage of CD4(+); T cells was increased in neonatal cord blood(P<0.01); the percentage of CD4(+);CD25(+);/CD4(+); and CD4(+);CD25(high);/CD4(+);T cells were decreased in neonatal cord blood(P<0.05). The percentage of Foxp3(+); cells in CD4(+);CD25(+); and CD4(+);CD25(high); T cells in neonatal cord blood were both lower than that in adult peripheral blood(P<0.01) and the expression level of Foxp3 mRNA was also lower than that in adult peripheral blood(P<0.05).
  
  
  CONCLUSIONS
  There are certain amount CD4(+);CD25(high); Tregs in neonatal cord blood, but the expression levels of Foxp3 are lower than that in adult peripheral blood, which indicate that Tregs might play a distinct role of immunoloregulation.
authors: Department of Hematology and Rheumatics, Jilin Provincial Hospital, Changchun 130021, China.
authors: Xue-Ye
authors: XY
authors: Wang
authors: Mei
authors: M
authors: Han
authors: Zhong-Ping
authors: ZP
authors: Xiao
authors: Lin
authors: L
authors: Chen
authors: Ling-Huan
authors: LH
authors: Kong
authors: Kun
authors: K
authors: Fu
authors: Tao
authors: T
authors: Feng
authors: Ling-Jun
authors: LJ
authors: Li
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005312:Fetal Blood / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D051858:Forkhead Transcription Factors