Asymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes.
Proteo-giant liposomes were electroformed from a mixture of lecithin vesicles and inside-out vesicles from erythrocytes. After addition of Mg-ATP in the vicinity of the proteo-giant liposomes, small buds appeared on the liposome surfaces, which--via an increase in lipids in the outer monolayer--demonstrated the active transport of lipids from the inner to the outer monolayer, indicating flippase activity.
Ezanno
Pierre
P
Institut de Biologie Physico-Chimique, CNRS, UMR7099, 13 rue Pierre et Marie Curie, 75005, Paris, France.
Cribier
Sophie
S
Devaux
Philippe F
PF
D000255:Adenosine Triphosphate / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism*; D005453:Fluorescence; D054709:Lecithins / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012964:Sodium / Q000378:metabolism; D013314:Stress, Mechanical; D013500:Surface Tension; D053835:Unilamellar Liposomes / Q000378:metabolism*
D054709:Lecithins; D053835:Unilamellar Liposomes; D000255:Adenosine Triphosphate; D012964:Sodium; D008274:Magnesium