[1H-NMR-based metabonomics analysis of plasma from osteoporotic rats induced by ovariectomy].
OBJECTIVE
To investigate the application of metabonomics in research of osteoporosis, through detecting change of the endogenous metabolites in plasma from osteoporotic rats by ovariectomy.


METHODS
Six old-months female SD rats were randomly divided into sham and OVX group. Fifth month after ovariectomy, plasma were collected from both groups, respectively. The metabolic profiles were investigated using 1H-NMR spectroscopy of plsama combined with pattern recognition techniques, including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).


RESULTS
The PCA PLS-DA plots of the plasma samples presented marked clustering between sham group and OVX group. Compared to sham group, the level of low molecular metabolites such as lactate, acetone and ethonal were higer, glucose, choline/phosphatidylcholine (Cho/PC), alanine (Ala), high density lipoprotein/low density lipoprotein (HDL/LDL), very low density lipoprotein/low density lipoprotein, fatty acid and glucose were lower.


CONCLUSIONS
Obvious changes in metabonomics of plasma from osteoporotic rats were observed.
Long
Wei-Fu
WF
Biomedical Engneering Institute, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Li
Liang
L
Chen
Huai-Qing
HQ
Tang
Yong
Y
He
Xue-Ling
XL
Jing
Rong-Zhou
RZ
D000818:Animals; D005260:Female; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D010024:Osteoporosis / Q000097:blood* / Q000209:etiology; D010052:Ovariectomy; D010949:Plasma / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley