[Genetic diversity of ixodid tick-borne pathogens in Tomsk City and suburbs].
We studied two urban and two suburban biotypes of Tomsk City for tick-transmitted diseases prevalence in naturally collected ticks. Tick-borne encephalitis virus (TBEV) was found in 6.5% of tick samples, West Nile virus (WNV) in 2.2%, Borrelia spp. in 8%, Rickettsia spp. in 2.5%, and Ehrlichia spp. in 1.7% of samples. Genetic markers of Powassan virus, Bartonella spp., and Balbesia spp. were not found. Analysis of the genetic diversity of revealed pathogens resulted in the following conclusions: 1. TBEV strains belong to Siberian and Far-Eastern subtypes, and Far-Eastern subtype of TBEV is most frequent in urban biotypes (up to 43 % of urban strains of TBEV); 2. WNV strains belong to genotype la; 3. Borrelia spp. were classified as B. garinii; 4. Rickettsia spp. were classified as R. tarasevichiae and probably as a new Rickettsia raoultii subspecies; 5. Ehrlichia spp. were classified as E. muris. The coexistence of several pathogens was found in 5.7% of tick samples, and the most frequent combination was TBEV + Borrelia spp.
Chausov
E V
EV
Ternovoĭ
V A
VA
Protopopova
E V
EV
Konovalova
S N
SN
Kononova
Iu V
IuV
Pershikova
N L
NL
Moskovitina
N S
NS
Romanenko
V N
VN
Ivanova
N V
NV
Bol'shakova
N P
NP
Moskvitin
S S
SS
Korobitsyn
I G
IG
Gashkov
S I
SI
Tiuten'kov
O Iu
OIu
Kuranova
V N
VN
Kravchenko
L B
LB
Suchkova
N G
NG
Agulova
L P
LP
Loktev
V B
VB
D000818:Animals; D001898:Borrelia / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004536:Ehrlichia / Q000145:classification / Q000235:genetics*; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D026863:Ixodidae / Q000382:microbiology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012281:Rickettsia / Q000145:classification / Q000235:genetics*; D012800:Siberia; D014902:West Nile virus / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial; D012367:RNA, Viral