title: Characterization of the ars gene cluster from extremely arsenic-resistant Microbacterium sp. strain A33.
abstract: The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Escherichia coli ars mutant. ArsRC2 negatively regulates the expression of the pentacistronic operon. ArsC1 and ArsC3 are related to thioredoxin-dependent arsenate reductases; however, ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes.
authors: IEBE, UMR 7146 CNRS Université Paul Verlaine-Metz, Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors: Asma
authors: A
authors: Achour-Rokbani
authors: Audrey
authors: A
authors: Cordi
authors: Pascal
authors: P
authors: Poupin
authors: Pascale
authors: P
authors: Bauda
authors: Patrick
authors: P
authors: Billard
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D053502:Arsenate Reductases / Q000235:genetics / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D053501:Arsenite Transporting ATPases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D017422:Sequence Analysis, DNA; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D013879:Thioredoxins; D053502:Arsenate Reductases; D053501:Arsenite Transporting ATPases; D001151:Arsenic