Recognition and sensing of nucleoside monophosphates by a dicopper(II) cryptate.
The dimetallic cryptate [Cu(2)(II)(1)](4+) selectively recognizes guanosine monophosphate with respect to other nucleoside monophosphates (NMPs) in a MeOH/water solution at pH 7. Recognition is efficiently signaled through the displacement of the indicator 6-carboxyfluorescein bound to the receptor, monitoring its yellow fluorescent emission. Titration experiments evidenced the occurrence of several simultaneous equilibria involving 1:1 and 2:1 receptor/NMP and receptor/indicator complexes. It was demonstrated that the added NMP displaces the indicator from the 2:1 receptor/indicator complex, forming the 1:1 receptor/analyte inclusion complex. Recognition selectivity is thus ascribed to the nature of nucleotide donor atoms involved in the coordination and their ability to encompass the Cu(II)-Cu(II) distance within the cryptate.
Amendola
Valeria
V
Dipartimento di Chimica Generale, Università di Pavia, via Taramelli 12, 27100 Pavia, Italy.
Bergamaschi
Greta
G
Buttafava
Armando
A
Fabbrizzi
Luigi
L
Monzani
Enrico
E
D003300:Copper / Q000737:chemistry*; D043844:Crown Ethers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D009584:Nitrogen / Q000737:chemistry; D009711:Nucleotides / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry; D013379:Substrate Specificity; D014867:Water / Q000737:chemistry
D043844:Crown Ethers; D005456:Fluorescent Dyes; D009711:Nucleotides; D009942:Organometallic Compounds; D014867:Water; D003300:Copper; D009584:Nitrogen; D010100:Oxygen; D000432:Methanol