title: Targeted delivery of daunorubicin to T-cell acute lymphoblastic leukemia by aptamer.
abstract: Application of daunorubicin in treatment of leukemia has been limited for its side effects like cardiotoxicity. Specific delivery of chemotherapy drugs is an important factor in decreasing their side effects. In this study, sgc8, an aptamer for protein tyrosine kinase-7 (PTK7), was used for specific delivery of daunorubicin to Molt-4 cells (PTK7(+)). Flow cytometric experiments showed that aptamer-daunorubicin complex was internalized effectively to Molt-4 cells (PTK7(+)), but not to U266 cells (PTK7(-)). This fact was confirmed by less cytotoxicity of aptamer-drug complex in U266 cells in compare to daunorubicin alone. No significant change in viability between daunorubicin and aptamer-daunorubicin complex treated Molt4 cells was observed. In conclusion, sgc8-daunorubicin complex is introduced as a simple and efficient system for targeted delivery of drug to acute lymphoblastic leukemia T cells.
authors: Department of Pharmaceutical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Seyed Mohammad
authors: SM
authors: Taghdisi
authors: Khalil
authors: K
authors: Abnous
authors: Fatemeh
authors: F
authors: Mosaffa
authors: Javad
authors: J
authors: Behravan
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D045744:Cell Line, Tumor; D003630:Daunorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006801:Humans; D009690:Nucleic Acid Conformation; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D003630:Daunorubicin