UV-vis, IR and 1H NMR spectroscopic studies and characterization of ionic-pair crystal violet-oxytetracycline.
The present study shows the formation and characterization of the ionic-pair between the antibiotic oxytetracycline and the dye crystal violet in ammonia solution pH 9.0+/-0.2 extracted into chloroform. The characterization was demonstrated using UV-vis spectrophotometry, (1)H NMR, measurement of relaxation times T(1) and IR spectroscopy, using a comparison between the signals of individual pure compounds with the signals with the mixture CV-OTC in different alkaline media. The formation of ionic-pair was also corroborated by new signals and chemical shifts. (2D) NMR spectroscopy experiments show that the interaction is electrostatic.
Orellana
Sandra
S
Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile. analitic@uchile.cl
Soto
César
C
Toral
M Inés
MI
D000890:Anti-Infective Agents / Q000737:chemistry*; D005840:Gentian Violet / Q000737:chemistry*; D006801:Humans; D007477:Ions / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010118:Oxytetracycline / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D055672:Static Electricity
D000890:Anti-Infective Agents; D007477:Ions; D005840:Gentian Violet; D010118:Oxytetracycline