Expression of NGF and GDNF family members and their receptors during peripheral nerve development and differentiation of Schwann cells in vitro.
Ligands of NGF and GDNF families of neurotrophic factors have important functions in the development of the vertebrate peripheral nervous system (PNS). It has been established that they also play key roles in the regeneration of PNS. Expression patterns of NGF and GDNF family members and their receptors have mostly been analyzed during regeneration, and less during development of the PNS. We describe the expression of mRNAs encoding these neurotrophic factors and their receptors during development of rat sciatic nerve and in three modes of differentiation of cultured rat Schwann cells. Our results demonstrate specific expression patterns of NGF and GDNF family ligands and their receptors during differentiation of Schwann cells in vivo and in vitro.
Piirsoo
Marko
M
Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Kaljas
Anne
A
Tamm
Karin
K
Timmusk
Tõnis
T
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000096:biosynthesis; D020932:Nerve Growth Factor / Q000096:biosynthesis*; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis*; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor