title: Carboxymethylation of the fibrillar collagen with respect to formation of hydroxyapatite.
abstract: Control over crystal growth by acidic matrix macromolecules is an important process in the formation of many mineralized tissues. Highly acidic macromolecules are postulated intermediates in tissue mineralization, because they sequester many calcium ions and occur in high concentrations at mineralizing foci in distantly related organisms. A prerequisite for biomineralization is the ability of cations like calcium to bind to proteins and to result in concert with appropriate anions like phosphates or carbonates in composite materials with bone-like properties. For this mineralization process the proteins have to be modified with respect to acidification. In this study we modified the protein collagen by carboxymethylation using glucuronic acid. Our experiments showed unambigously, that N(epsilon)-carboxymethyllysine is the major product of the in vitro nonenzymatic glycation reaction between glucuronic acid and collagen. We hypothesized that the function of biomimetically carboxymethylated collagen is to increase the local concentration of corresponding ions so that a critical nucleus of ions can be formed, leading to the formation of the mineral. Thus, the self-organization of HAP nanocrystals on and within collagen fibrils was intensified by carboxymethylation.
authors: Institute of Bioanalytical Chemistry, TU Dresden, Dresden, 01069 Germany. Hermann.Ehrlich@tu-dresden.de
authors: Hermann
authors: H
authors: Ehrlich
authors: Thomas
authors: T
authors: Hanke
authors: Paul
authors: P
authors: Simon
authors: René
authors: R
authors: Born
authors: Christiane
authors: C
authors: Fischer
authors: Andrej
authors: A
authors: Frolov
authors: Tobias
authors: T
authors: Langrock
authors: Ralf
authors: R
authors: Hoffmann
authors: Uwe
authors: U
authors: Schwarzenbolz
authors: Thomas
authors: T
authors: Henle
authors: Vasily V
authors: VV
authors: Bazhenov
authors: Hartmut
authors: H
authors: Worch
mesh_terms: D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D032701:Biomimetics; D001894:Borohydrides / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization; D005947:Glucose / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006038:Glyoxylates / Q000737:chemistry; D006882:Hydroxyapatites / Q000737:chemistry*; D007202:Indicators and Reagents; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry; D008745:Methylation; D020894:Microfibrils; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000596:Amino Acids; D001894:Borohydrides; D006038:Glyoxylates; D006882:Hydroxyapatites; D007202:Indicators and Reagents; D008903:Minerals; C048496:N(6)-carboxymethyllysine; D020723:Glucuronic Acid; D003094:Collagen; C009282:sodium cyanoborohydride; D005947:Glucose; C031150:glyoxylic acid; D008239:Lysine