In vitro antifungal action of different substances over microwaved-cured acrylic resins.
OBJECTIVE
The presence of Candida albicans on the surfaces of denture-base acrylic resins is strongly related to the development of oral stomatitis. This study evaluated the antifungal action of different agents over microwave-cured acrylic resin without polishing specimens previously contaminated with Candida albicans.


METHODS
Sixty specimens were immersed in BHI broth previously inoculated with the yeast and stored for 3 h at 37 degrees C. They were divided into 5 experimental groups (n=10): G1: 2% chlorhexidine solution (10 min); G2: 0.5% sodium hypochlorite (10 min); G3: modified sodium hypochlorite (10 min); G4: effervescent agent (5 min); G5: hydrogen peroxide 10 v (30 min). The specimens of the control group 1 (C1) were not disinfected. Ten additional specimens of the control group 2 (C2) were not infected with the yeast, aiming to check the asepsis during the experiment. The disinfection agents were neutralized and the acrylic resin specimens were immersed in BHI Broth for 24 h. Culture media turbidity was evaluated spectrophotometrically according to the transmittance degree, i.e. the higher the transmittance the stronger the antimicrobial action. Statistical analysis was performed (Kruskal-Wallis Test, p<0.05).


RESULTS
The results, represented by the medians, were: G1 = 40; G2 = 100; G3 = 100; G4 = 90; G5 = 100; C1 = 40; C2 = 100.


CONCLUSIONS
This in vitro study suggested that sodium hypochlorite-based substances and hydrogen peroxide are more efficient disinfectants against C. albicans than 2% chlorhexidine solution and the effervescent agent.
Montagner
Henrique
H
Department of Restorative Dentistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Montagner
Francisco
F
Braun
Katia Olmedo
KO
Peres
Paulo Edelvar Correa
PE
Gomes
Brenda Paula Figueiredo de Almeida
BP
D000180:Acrylic Resins / Q000737:chemistry / Q000528:radiation effects*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002710:Chlorhexidine / Q000494:pharmacology; D019551:Dental Disinfectants / Q000494:pharmacology*; D003764:Dental Materials / Q000737:chemistry / Q000528:radiation effects*; D003820:Denture Cleansers / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008422:Materials Testing; D008872:Microwaves; D016877:Oxidants / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013053:Spectrophotometry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D000180:Acrylic Resins; D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D019551:Dental Disinfectants; D003764:Dental Materials; D003820:Denture Cleansers; D016877:Oxidants; C540407:corega tabs; D000431:Ethanol; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D002710:Chlorhexidine