title: RNA induced polymerization of the Borna disease virus nucleoprotein.
abstract: The Borna disease virus (BDV) nucleoprotein (N) monomer resembles the nucleoprotein structures from rabies virus (RABV) and vesicular stomatitis virus (VSV). We show that BDV N assembles into ring- and string-like structures in the presence of 5' genomic BDV RNA. RNA induced polymerization is partly RNA-specific since polymerization is inefficient in the presence of 3' genomic BDV RNA or E. coli RNA. Mutagenesis of basic residues located in the cleft made up by the N- and C-terminal domains of N abrogate RNA-induced polymerization indicating that BDV N binds RNA similarly as observed in case of RABV and VSV N-RNA complexes. Bound RNA is not protected and sensitive to degradation. N-RNA polymers form complexes with the phosphoprotein P as required for functional transcription or replication units. Our data indicate that BDV N utilizes similar structural principles for N-RNA and N-P-RNA complex formation as observed for related negative strand RNA viruses.
authors: Unit of Virus Host Cell Interactions (UVHCI) UMI 3265 Université Joseph Fourier-EMBL-CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France.
authors: Miriam
authors: M
authors: Hock
authors: Ina
authors: I
authors: Kraus
authors: Guy
authors: G
authors: Schoehn
authors: Marc
authors: M
authors: Jamin
authors: Cornelia
authors: C
authors: Andrei-Selmer
authors: Wolfgang
authors: W
authors: Garten
authors: Winfried
authors: W
authors: Weissenhorn
mesh_terms: D001665:Binding Sites; D001891:Borna disease virus / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009698:Nucleoproteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D055503:Protein Multimerization; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D009698:Nucleoproteins; C496537:P protein, Borna disease virus; D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins