A Sabin 2-related poliovirus recombinant contains a homologous sequence of human enterovirus species C in the viral polymerase coding region.
A type 2 vaccine-related poliovirus (strain CHN3024), differing from the Sabin 2 strain by 0.44% in the VP1 coding region was isolated from a patient with vaccine-associated paralytic poliomyelitis. Sequences downstream of nucleotide position 6735 (3D(pol) coding region) were derived from an unidentified sequence; no close match for a potential parent was found, but it could be classified into a non-polio human enteroviruses species C (HEV-C) phylogeny. The virus differed antigenically from the parental Sabin strain, having an amino acid substitution in the neutralizing antigenic site 1. The similarity between CHN3024 and Sabin 2 sequences suggests that the recombination was recent; this is supported by the estimation that the initiating OPV dose was given only 36-75 days before sampling. The patient's clinical manifestations, intratypic differentiation examination, and whole-genome sequencing showed that this recombinant exhibited characteristics of neurovirulent vaccine-derived polioviruses (VDPV), which may, thus, pose a potential threat to a polio-free world.
Zhang
Yong
Y
WHO WPRO Regional Polio Reference Laboratory and State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 27, Nanwei Road, Xuanwu District, 100050, Beijing, People's Republic of China.
Zhang
Fan
F
Zhu
Shuangli
S
Chen
Li
L
Yan
Dongmei
D
Wang
Dongyan
D
Tang
Ruiyan
R
Zhu
Hui
H
Hou
Xiaohui
X
An
Hongqiu
H
Zhang
Hong
H
Xu
Wenbo
W
D019943:Amino Acid Substitution / Q000235:genetics; D016000:Cluster Analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D000939:Epitopes / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D020125:Mutation, Missense; D010802:Phylogeny; D011051:Poliomyelitis / Q000821:virology; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*
D000939:Epitopes; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases