Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells.
We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
Park
YongKeun
Y
George R. Harrison Spectroscopy Laboratory, MIT, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Yamauchi
Toyohiko
T
Choi
Wonshik
W
Dasari
Ramachandra
R
Feld
Michael S
MS
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002417:Cattle; D048429:Cell Size; D002470:Cell Survival; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008853:Microscopy; D013057:Spectrum Analysis
D006454:Hemoglobins