Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone.
In chickens, corticosterone is the end-product of stress. However, the nature of the immune response to elevated plasma corticosterone concentrations at the molecular level has not yet been characterised. We recently demonstrated that exposure to corticosterone in drinking water for 1 week significantly upregulates mRNA expression levels for the pro-inflammatory interleukins (IL)-1beta, IL-6, IL-18 and the pro-inflammatory chemokine CCLi2 in chicken lymphocytes, particularly 3 h after the treatment started. In the present study, we investigated cytokine and chemokine mRNA expression levels in circulating heterophils of chickens, and show that at 3 h post initial treatment with corticosterone in drinking water (20 mg/1L) the mRNA expression levels for IL-1beta, IL-6, IL-10, IL-12alpha and IL-18 are upregulated. The mRNA expression levels for IL-6, IL-10 and IL-18 correlate with plasma corticosterone concentration and total heterophil counts. Corticosterone downregulated the expression levels of all pro-inflammatory cytokines at 24 h and 1 week post-treatments. Repeated treatment with corticosterone upregulated mRNA expression levels of transforming growth factor-beta4 and the chemokine CCL16. These data indicate that cytokine and chemokine gene expression signatures in chicken heterophils can be altered during stress and therefore could be used as an indicator of stress.
Shini
Shaniko
S
School of Veterinary Science & School of Animal Studies, University of Queensland, Gatton, Australia. s.shini@uq.edu.au
Shini
Agim
A
Kaiser
Pete
P
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000494:pharmacology*; D018932:Chemokine CCL2 / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics; D054360:Chemokine CXCL1 / Q000235:genetics; D002645:Chickens; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects* / Q000276:immunology; D020382:Interleukin-18 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007958:Leukocyte Count; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054360:Chemokine CXCL1; D016207:Cytokines; D020382:Interleukin-18; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D003345:Corticosterone