Characterization of the ars gene cluster from extremely arsenic-resistant Microbacterium sp. strain A33.
The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Escherichia coli ars mutant. ArsRC2 negatively regulates the expression of the pentacistronic operon. ArsC1 and ArsC3 are related to thioredoxin-dependent arsenate reductases; however, ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes.
Achour-Rokbani
Asma
A
IEBE, UMR 7146 CNRS Université Paul Verlaine-Metz, Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
Cordi
Audrey
A
Poupin
Pascal
P
Bauda
Pascale
P
Billard
Patrick
P
D000192:Actinomycetales / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D053502:Arsenate Reductases / Q000235:genetics / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D053501:Arsenite Transporting ATPases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D017422:Sequence Analysis, DNA; D013879:Thioredoxins / Q000378:metabolism
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D013879:Thioredoxins; D053502:Arsenate Reductases; D053501:Arsenite Transporting ATPases; D001151:Arsenic