title: Fine mapping of a gene causing hybrid pollen sterility between Yunnan weedy rice and cultivated rice (Oryza sativa L.) and phylogenetic analysis of Yunnan weedy rice.
abstract: Weedy rice represents an important resource for rice improvement. The F(1) hybrid between the japonica wide compatibility rice cultivar 02428 and a weedy rice accession from Yunnan province (SW China) suffered from pollen sterility. Pollen abortion in the hybrid occurred at the early bicellular pollen stage, as a result of mitotic failure in the microspore, although the tapetum developed normally. Genetic mapping in a BC(1)F(1) population (02428//Yunnan weedy rice (YWR)/02428) showed that a major QTL for hybrid pollen sterility (qPS-1) was present on chromosome 1. qPS-1 was fine-mapped to a 110 kb region known to contain the hybrid pollen sterility gene Sa, making it likely that qPS-1 is either identical to, or allelic with Sa. Interestingly, F(1) hybrid indicated that Dular and IR36 were assumed to carry the sterility-neutral allele, Sa ( n ). Re-sequencing SaM and SaF, the two component genes present at Sa, suggested that variation for IR36 and Dular may be responsible for the loss of male sterility, and the qPS-1 sequence might be derived from wild rice or indica cultivars. A phylogenetic analysis based on microsatellite genotyping suggested that the YWR accession is more closely related to wild rice and indica type cultivars than to japonica types. Thus it is probable that the YWR accession evolved from a spontaneous hybrid between wild rice and an ancient cultivated strain of domesticated rice.
authors: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, 210095 Nanjing, China.
authors: Yong
authors: Y
authors: Wang
authors: Zheng Zheng
authors: ZZ
authors: Zhong
authors: Zhi Gang
authors: ZG
authors: Zhao
authors: Ling
authors: L
authors: Jiang
authors: Xiao Feng
authors: XF
authors: Bian
authors: Wen Wei
authors: WW
authors: Zhang
authors: Ling Long
authors: LL
authors: Liu
authors: H
authors: H
authors: Ikehashi
authors: Jian Min
authors: JM
authors: Wan
mesh_terms: D002874:Chromosome Mapping; D005298:Fertility / Q000235:genetics; D005838:Genotype; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D011058:Pollen / Q000235:genetics* / Q000502:physiology; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci