Isolation and spatial expression analysis of a partial esterase gene of Culex nigripalpus (Diptera: Culicidae).
Mosquitoes play an important role as vectors for a variety of pathogens. Insecticide use for control means results in selection of tolerant/resistant mosquitoes. An esterase cDNA was isolated from Culex nigripalpus Theobald (Diptera: Culicidae), and its spatial expression was characterized. GenBank tblastx and VectorBase searches with the translation product of the longest cDNA fragment (397 bp, CN Temsha est-1) of the Cx. nigripalpus esterase revealed similarity (95%) to the estalpha2 esterase gene product of Culex pipiens quinquefasciatus Say, suggesting it is a member of the esterase family. Transcripts encoding Cx. nigripalpus esterase were detected in midgut tissue, thoraces, and abdomens of female Cx. nigripalpus, findings that may support possible roles in feeding, digestion, or reproduction. Gene expression studies to determine the role of Cx. nigripalpus esterase in the mosquito are being conducted.
Eans
Shainnel
S
Florida Medical Entomology Laboratory, University of Florida, 200 9th St. SE, Vero Beach, FL 32962, USA.
Frommer
Robert L
RL
Smartt
Chelsea T
CT
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003465:Culex / Q000201:enzymology / Q000235:genetics*; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D019476:Insect Proteins; D004950:Esterases