[Molecular markers associated with prostate cancer: 3-nitrotyrosine and genetic and proteic expression of Mn-superoxide dismutasa (Mn-SOD)].
OBJECTIVE
Recent reports place prostate cancer (PCa) as third cause of death in the World among males, in Mexico it is the leading cause of male death. In this study, we studied molecular markers and one genetic marker related in tissues with PCa and benign prostatic hyperplasia (BPH): The Mn-superoxide dismutase (Mn-SOD) gen and protein, prostate specific antigen (PSA) and 3-nitrotyrosine (3-NT). It was evaluated if various markers of oxidative stress show altered expression in prostate cancer and BPH.


METHODS
80 biopsies were obtained. The conditions to amplify and evaluate the immunoreactivity of the genes of interest (Mn-SOD and 3-NT) and the correlation between PSA and Mn-SOD immunoreactivity in prostate cancer and benign prostatic hyperplasia were standardized.


RESULTS
Gene overexpression and Mn-SOD and 3-NT immunoreactivity were greater in prostate cancer with respect to the BPH group. Correlation between levels of PSA and the Mn-SOD immunoreactivity was not observed.


CONCLUSIONS
The above results suggest that the parameters evaluated can be used as tumor markers making the determinations in biopsies of patients suspected of prostate cancer.
Floriano-Sánchez
Esaú
E
Departamento de Bioquímica y Biología Molecular, Escuela Médico Militar, México, DF. floriano_esa@yahoo.com
Castro-Marín
Melchor
M
Cárdenas-Rodríguez
Noemí
N
D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis
D005819:Genetic Markers; C002744:3-nitrotyrosine; D014443:Tyrosine; D013482:Superoxide Dismutase