Use of Hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters.
Filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-microm-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry.
Lee
A
A
Office of Biotechnology Products, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA.
McVey
J
J
Faustino
P
P
Lute
S
S
Sweeney
N
N
Pawar
V
V
Khan
M
M
Brorson
K
K
Hussong
D
D
D000393:Air Pollutants; D001419:Bacteria; D001431:Bacteriological Techniques / Q000295:instrumentation; D015169:Colony Count, Microbial; D042621:Comamonadaceae / Q000648:ultrastructure; D003470:Culture Media; D004203:Disinfection / Q000295:instrumentation; D004340:Drug Contamination; D004345:Drug Industry / Q000295:instrumentation; D004784:Environmental Monitoring / Q000295:instrumentation; D005374:Filtration / Q000295:instrumentation*; D005618:Fresh Water; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D013242:Sterilization / Q000295:instrumentation; D014871:Water Microbiology; D018508:Water Purification / Q000295:instrumentation
D000393:Air Pollutants; D003470:Culture Media; D008567:Membranes, Artificial