title: Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor.
abstract: A low-cost microBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a microBR, a conventional bench scale bioreactor and a shake flask. Under the microBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 microg/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the microBR were comparable to those obtained using conventional scale methods.
authors: Chemical and Bioprocess Engineering Group, School of Science and Engineering, Teesside University, Middlesbrough TS1 3BA, Tees Valley, UK. p.rahman@tees.ac.uk
authors: Pattanathu K S M
authors: PK
authors: Rahman
authors: Godfrey
authors: G
authors: Pasirayi
authors: Vincent
authors: V
authors: Auger
authors: Zulfiqur
authors: Z
authors: Ali
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D019149:Bioreactors; D004867:Equipment Design; D006017:Glycolipids / Q000378:metabolism*; D044085:Microfluidics / Q000295:instrumentation*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011710:Pyocyanine / Q000378:metabolism* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; D011710:Pyocyanine