Noise induced hearing loss and vestibular dysfunction in the guinea pig.
This study analysed the acoustic and vestibular functional and morphological modifications in guinea pigs after acoustic trauma. Animals were exposed to noise (6 kHz, at 120 dB SPL for 60 minutes) and then auditory brainstem responses (ABR) and vestibuloocular reflex (VOR) were measured at 6 hours, 1 day, 3, 7, and 21 days after noise. Western blotting and immunostaining for 4-hydroxy-2-noneal (4-HNE) and vascular endothelial growth factor (VEGF) were performed in the cochlear and vestibular regions at 1 and 7 days after noise exposure. A significant decrease of VOR gain was observed on day 1 and the recovery was completed at day 21. ABR threshold values reached a level of 80 dB at day 1 after trauma reaching a value of about 50 dB SPL on day 21. 4-HNE expression, a marker of lipid peroxidation was strongly increased in the cochlea. In the vestibule, 4-HNE immunoreactivity was faint. However, VEGF was up-regulated both in the cochlea and vestibule. In conclusion, the expression of VEGF in both cochlear and vestibular structures suggests a reparative role with potentially therapeutic implications.
Fetoni
Anna Rita
AR
Institute of Otolaryngology, Catholic University, Rome, Italy. afetoni@rm.unicatt.it
Ferraresi
Aldo
A
Picciotti
Pasqualina
P
Gaetani
Eleonora
E
Paludetti
Gaetano
G
Troiani
Diana
D
D000447:Aldehydes / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003051:Cochlea / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007150:Immunohistochemistry; D012027:Reflex, Vestibulo-Ocular; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000096:biosynthesis; D014722:Vestibule, Labyrinth / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
D000447:Aldehydes; D042461:Vascular Endothelial Growth Factor A; C027576:4-hydroxy-2-nonenal