title: Cyclosporine a-loaded solid lipid nanoparticles: ocular tolerance and in vivo drug release in rabbit eyes.
abstract: OBJECTIVE
  To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes.
  
  
  METHODS
  SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing.
  
  
  RESULTS
  The particle size of SLNs was detected as 225.9 +/- 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes.
  
  
  CONCLUSIONS
  Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, Turkey. evren.homan.gokce@ege.edu.tr
authors: Evren H
authors: EH
authors: Gökçe
authors: Giuseppina
authors: G
authors: Sandri
authors: Sait
authors: S
authors: Eğrilmez
authors: M Cristina
authors: MC
authors: Bonferoni
authors: Tamer
authors: T
authors: Güneri
authors: Carla
authors: C
authors: Caramella
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001682:Biological Availability; D001693:Biological Transport, Active; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D008055:Lipids / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011817:Rabbits
chemical_list: D007166:Immunosuppressive Agents; D008055:Lipids; D016572:Cyclosporine