Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach.
Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.
Barranca
Marilisa
M
Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Via Divisi 83, 90133 Palermo, Italy.
Fontana
Simona
S
Taverna
Simona
S
Duro
Giovanni
G
Zanella-Cleon
Isabelle
I
Becchi
Michel
M
De Leo
Giacomo
G
Alessandro
Riccardo
R
D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology / Q000378:metabolism; D032403:Parietaria / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011058:Pollen / Q000737:chemistry* / Q000276:immunology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D052179:Antigens, Plant; D010940:Plant Proteins; D020543:Proteome; D007073:Immunoglobulin E