Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum.
BACKGROUND
Probiotic microorganisms favorably alter the intestinal microflora balance, promote intestinal integrity and mobility, inhibit the growth of harmful bacteria and increase resistance to infection. Probiotics are increasingly used in nutraceuticals, functional foods or in microbial interference treatment. However, the effectiveness of probiotic organism is considered to be population-specific due to variation in gut microflora, food habits and specific host-microbial interactions. Most of the probiotic strains available in the market are of western or European origin, and a strong need for exploring new indigenous probiotic organisms is felt.


RESULTS
An indigenous isolate Lp9 identified as Lactobacillus plantarum by molecular-typing methods was studied extensively for its functional and probiotic attributes, viz., acid and bile salt tolerance, cell surface hydrophobicity, autoaggregation and Caco-2 cell-binding as well as antibacterial and antioxidative activities. Lp9 isolate could survive 2 h incubation at pH 1.5-2.0 and toxicity of 1.5-2.0% oxgall bile. Lp9 could deconjugate major bile salts like glycocholate and deoxytaurocholate, indicating its potential to cause hypocholesterolemia. The isolate exhibited cell-surface hydrophobicity of approximately 37% and autoaggregation of approximately 31%. Presence of putative probiotic marker genes like mucus-binding protein (mub), fibronectin-binding protein (fbp) and bile salt hydrolase (bsh) were confirmed by PCR. Presence of these genes suggested the possibility of specific interaction and colonization potential of Lp9 isolate in the gut, which was also suggested by a good adhesion ratio of 7.4+/-1.3% with Caco-2 cell line. The isolate demonstrated higher free radical scavenging activity than standard probiotics L. johnsonii LA1 and L. acidophilus LA7. Lp9 also exhibited antibacterial activity against E. coli, L. monocytogenes, S. typhi, S. aureus and B. cereus.


CONCLUSIONS
The indigenous Lactobacillus plantarum Lp9 exhibited high resistance against low pH and bile and possessed antibacterial, antioxidative and cholesterol lowering properties with a potential for exploitation in the development of indigenous functional food or nutraceuticals.
Kaushik
Jai K
JK
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, India. Jaikr1@gmail.com
Kumar
Ashutosh
A
Duary
Raj K
RK
Mohanty
Ashok K
AK
Grover
Sunita
S
Batish
Virender K
VK
D000222:Adaptation, Physiological / Q000187:drug effects; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001647:Bile Acids and Salts / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001647:Bile Acids and Salts; D000581:Amidohydrolases; C023154:choloylglycine hydrolase