[Pteridine-dependent oxygen activation in neutrophils].
We investigated the influence ofneopterin and 7, 8-dihydroneopterin on the activity and secretory degranulation of myeloperoxidase in neutrophils and the ability of pteridines to interact with the main substrate of this enzyme, hydrogen peroxide, and with the intermediate product of halogenation cycle--hypochlorous acid. It was shown that neopterin and 7, 8-dihydroneopterin while being a redox-pair regulated the process of oxygen activation in neutrophils by functioning of myeloperoxidase. Depending on concentration, pteridines can influence the secretion of myeloperoxidase into intracellular medium and decrease the level of hydrogen peroxide and hypochlorous acid that are a substrate and an intermediate product of the enzyme respectively. It was shown that 7, 8-dihydroneopterin in micromolar concentration appeared to be noncompetitive inhibitor of myeloperoxidase. We suppose that myeloperoxidase assists 7, 8-dihydroneopterin oxidation by hypochlorous acid that leads to neopterin concentration increase. These changes modify the concentration of reactive oxygen species in intracellular and extracellular media.
Petukh
M G
MG
Semenkova
G N
GN
Fuchs
D
D
Cherenkevich
S N
SN
D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006997:Hypochlorous Acid / Q000378:metabolism; D019798:Neopterin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; C000976:7,8-dihydroneopterin; D019798:Neopterin; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase