The effect of retroviral vector on uptake of human lactoferrin DNA by Yak (Bos Grunniens) spermatozoa and their fertilizability in vitro.
The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using PLNCX2-hLF+FuGene 6 to generate SMGT yak spermatozoa for fertilizing bovine oocytes. The result showed that DNA-binding rate increased with the extension of incubation period and DNA treatment did not decrease sperm motility. Oocytes inseminated with SMGT-spermatozoa had a lower (P < 0.05) cleavage rate (57.7%) than the control (73.4%), but development up to blastocyst stage was not different (26.8 vs. 31.7%). It appears that PLNCX2 is useful for generating transgenic yaks by SMGT.
Zi
Xiang-Dong
XD
The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, China. zixd2000@yahoo.com
Chen
Shao-Wei
SW
Liang
Guan-Nan
GN
Chen
Da-Wen
DW
Zhang
Da-Wei
DW
Yin
Rong-Hua
RH
D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D004247:DNA / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008297:Male; D012190:Retroviridae / Q000235:genetics*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*
D004247:DNA; D007781:Lactoferrin