title: Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes.
abstract: When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.
authors: U.S. Army Engineering Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61826, USA. clint.arnett@usace.army.mil
authors: Clint M
authors: CM
authors: Arnett
authors: Charles P
authors: CP
authors: Marsh
authors: Charles R
authors: CR
authors: Welch
authors: Michael S
authors: MS
authors: Strano
authors: Jae-Hee
authors: JH
authors: Han
authors: Jeffry H
authors: JH
authors: Gray
authors: Thomas A
authors: TA
authors: Carlson
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman; D013696:Temperature
chemical_list: D037742:Nanotubes, Carbon; D004247:DNA; D011088:DNA Ligases