title: [Evaluation of myocardial ischemia-reperfusion injury in mouse by molecular imaging of P-selectin with targeted contrast echocardiography].
abstract: OBJECTIVE
  To assess the feasibility of evaluating myocardial ischemia-reperfusion injury in mouse with targeted myocardial contrast echocardiography (MCE).
  
  
  METHODS
  Phospholipid microbubbles targeted to P-selectin (MBp) and control microbubbles (MBc) were created by conjugating monoclonal antibody against murine P-selectin or isotype control antibody with the lipid shell via "avidin-biotin" bridging. Ten mice with myocardial ischemia-reperfusion were injected intravenously of MBp and MBc in a random order with a 30 min interval. After 5 min of intravenous injection of microbubble, targeted MCE imaging was performed in all mice. And then the video intensity (VI) was determined.
  
  
  RESULTS
  A significant ultrasonic enhancement was observed in ischemic region of MBp-group. Increment in VI value of ischemic region in MBp-group was great and it amounted to (26.0 +/- 6.2) U. However, increment in VI value of ischemic region in MBc-group was minor and it was merely (9.1 +/- 0.9) U. Difference was evident in ischemic region between of two groups (P < 0.05). In both MBp-group and MBc-group, the VI value of ischemic region was significantly greater than that of non-ischemic region (6.5 +/- 1.0) U vs (6.4 +/- 0.8) U (P < 0.05). There was no obvious difference in the VI of non-ischemic region between two groups.
  
  
  CONCLUSIONS
  Molecular imaging of P-selectin with targeted contrast echocardiography can effectively evaluate myocardial ischemia-reperfusion injury.
authors: Cardiovascular Division, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Li-Jing
authors: LJ
authors: Ji
authors: Li
authors: L
authors: Yang
authors: Yi
authors: Y
authors: Yan
authors: Jue-Fei
authors: JF
authors: Wu
authors: Jun-Hua
authors: JH
authors: Li
authors: Guang-Quan
authors: GQ
authors: Hu
authors: Jian-Ping
authors: JP
authors: Bin
mesh_terms: D000818:Animals; D004562:Electrocardiography / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging*; D019007:P-Selectin; D014463:Ultrasonography
chemical_list: D019007:P-Selectin