Degradable polymers for gene delivery.
Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure led to significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than Lipofectamine 2000 or polyethylenimine for gene delivery to cancerous fibroblasts or human primary fibroblasts. These materials may be useful for cancer therapeutics and regenerative medicine.
Sunshine
Joel
J
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Bhise
Nupura
N
Green
Jordan J
JJ
D000818:Animals; D001673:Biodegradation, Environmental; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004337:Drug Carriers; D005347:Fibroblasts / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000295:instrumentation; D006801:Humans; D008055:Lipids / Q000737:chemistry; D009369:Neoplasms / Q000628:therapy*; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D044968:Regenerative Medicine / Q000379:methods
D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D011108:Polymers; D011094:Polyethyleneimine