Human respiratory syncytial virus reduces the number of cells in S-phase and increases GADD153 expression in HEp-2 cells.
Human respiratory syncytial virus (HRSV) associated with bronchiolitis and asthma is known to replicate actively in ciliated epithelial cells. However, little is known about the influence of HRSV replication on the cell cycle. We found that HRSV infection of HEp-2 cells led to a reduction of the number of cells in S-phase, to an increase in the number of cells in G1-phase, together with an increase of GADD153 mRNA levels and GADD153 protein expression. These results implied that a shorter S-phase supported HRSV replication suggesting possible strategies for interfering with productive HRSV infection.
Mohapatra
S
S
Department of Molecular Medicine, Joy McCann Culverhouse Airway Disease Center, College of Medicine, University of South Florida, VA Hospital, Tampa, Florida, USA.
Park
S J
SJ
Boyapalle
S
S
Pastey
M K
MK
Graham
B S
BS
Blanck
G
G
D002460:Cell Line; D016193:G1 Phase; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D018113:Respiratory Syncytial Virus, Human / Q000502:physiology*; D016196:S Phase; D051742:Transcription Factor CHOP / Q000235:genetics*; D014779:Virus Replication
C497249:DDIT3 protein, human; D012333:RNA, Messenger; D051742:Transcription Factor CHOP