Differently selected D-enantiomeric peptides act on different Abeta species.
Aging is the most significant risk factor for Alzheimer disease (AD). The pathological hallmark of AD is the accumulation of aggregated amyloid-beta (Abeta) forms and insoluble plaques, mainly composed of Abeta, in the brain of the patient. Recently, we reported on the selection of D-enantiomeric, Abeta-binding peptides D1 and D3. D1 was selected against aggregated Abeta species to address diagnosis by in vivo imaging of amyloid plaques, whereas D3 was selected using low-molecular-weight Abeta species, therefore addressing therapeutical studies. Here, we use a surface plasmon resonance method to confirm that both peptides show the desired binding specificities.
Bartnik
Dirk
D
Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
Funke
Susanne Aileen
SA
Andrei-Selmer
Luminita-Cornelia
LC
Bacher
Michael
M
Dodel
Richard
R
Willbold
Dieter
D
D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D000682:Amyloid / Q000032:analysis / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D058225:Plaque, Amyloid / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D055503:Protein Multimerization / Q000187:drug effects; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance
D000682:Amyloid; D016229:Amyloid beta-Peptides; C534952:D3 peptide; D009842:Oligopeptides; D010446:Peptide Fragments; D020033:Protein Isoforms