The role of nonbonded interactions in the conformational dynamics of organophosphorous hydrolase adsorbed onto functionalized mesoporous silica surfaces.
The enzyme organophosphorous hydrolase (OPH) catalyzes the hydrolysis of a wide variety of organophosphorous compounds with high catalytic efficiency and broad substrate specificity. The immobilization of OPH in functionalized mesoporous silica (FMS) surfaces increases significantly its catalytic specific activity, as compared to the enzyme in solution, with important applications for the detection and decontamination of insecticides and chemical warfare agents. Experimental measurements of immobilization efficiency as a function of the charge and coverage percentage of different functional groups have been interpreted as electrostatic forces being the predominant interactions underlying the adsorption of OPH onto FMS surfaces. Explicit solvent molecular dynamics simulations have been performed for OPH in bulk solution and adsorbed onto two distinct interaction potential models of the FMS functional groups to investigate the relative contributions of nonbonded interactions to the conformational dynamics and adsorption of the protein. Our results support the conclusion that electrostatic interactions are responsible for the binding of OPH to the FMS surface. However, these results also show that van der Waals forces are detrimental for interfacial adhesion. In addition, it is found that OPH adsorption onto the FMS models favors a protein conformation whose active site is fully accessible to the substrate, in contrast to the unconfined protein.
Gomes
Diego E B
DE
Pacific Northwest National Laboratory, P.O. Box 999, MSIN K7-90, Richland, Washington 99352, USA.
Lins
Roberto D
RD
Pascutti
Pedro G
PG
Lei
Chenghong
C
Soares
Thereza A
TA
D000327:Adsorption; D055162:Biocatalysis; D020134:Catalytic Domain; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D016062:Porosity; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases