Protein expression in human non-small cell lung cancer: a systematic database.
OBJECTIVE
The following database integrates results from published proteomics studies in human non-small cell lung cancer (NSCLC), with a focus on squamous cell cancers (SCC) and adenocarcinomas (AC).


METHODS
Only studies on NSCLC were analyzed. Results from 12 studies were available, 5 studies on SCC, 4 on AC, 1 on AC and SCC, and 2 on NSCLC without further distinction. Human cancer tissues, paired normal tissues, paired cancer patient's sera, normal sera or human tumor cell lines were analyzed. Three technologies were applied: two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), differential in-gel electrophoresis (DIGE) and serological proteome analysis (SERPA).


RESULTS
The total number of NSCLC patients was 306. Out of 261 differentially expressed proteins, 192 proteins (74%) were mentioned in a single study, 40 (15%) were mentioned by 2 different studies. 18 (7%) in 3 studies, 4 (2%) in 4 studies, 2 (1%) in 5 studies, and a single protein was listed in 6 studies.


CONCLUSIONS
Overlapping of protein expression is low between SCC and AC. However, a few proteins appear to be upregulated in SCC and AC: triosephosphate isomerase, protein disulfide isomerase and phosphoglycerate mutase (phosphoglycerate kinase 1). These three proteins might be key players in human lung cancer.
Bührens
Rena Isabel
RI
Department of General, Gastrointestinal and Thoracic Surgery, Evangelic Hospital Bielefeld, DE-33617 Bielefeld, Germany.
Amelung
Julius Titus
JT
Reymond
Marc André
MA
Beshay
Morris
M
D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D016208:Databases, Factual; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020407:Internet; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D016239:MEDLINE; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
D009363:Neoplasm Proteins