title: Screening of some Tanzanian medicinal plants for their trypanocidal and cytotoxic activities.
abstract: The objective of the present study was to evaluate in vitro antitrypanosomal and cytotoxic activities of crude extracts of 20 traditionally used medicinal plants of Tanzania. A total of 40 extracts (dichloromethane and methanol) were screened for antiproliferative activity of bloodstream form of T. b. brucei and human leukaemia HL-60 cell. Inhibition of cell proliferation was assessed using resazurin as vital stain. Of the 40 extracts tested, the dichloromethane extract from bark of Warburgia salutaris (Canellaceae) exhibited the most potent antitrypanosomal activity with an IC(50) value of 10.68 microg/ml. A dichloromethane extract from Lannea stuhlmannii (Anacardiaceae) was found to be the most cytotoxic extract against HL-60 (IC(50) = 27.15 microg/ml). Out of the 20 plants tested, 5 plants exhibited trypanocidal activity with IC(50) values below 20 microg/ml. These 5 plants: Entandrophragma bussei (Meliaceae), Securidaca longepedunculata (Polygalaceae), Warburgia salutaris (Canellaceae), Zanha africana (Sapindaceae) and Zanthoxylum chalybeum (Rutaceae) could therefore serve as sources of lead compounds for treatment of trypanosomiasis.
authors: Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Heidelberg, Germany.
authors: Endalkachew
authors: E
authors: Nibret
authors: Mohamed L
authors: ML
authors: Ashour
authors: Chrispinus D
authors: CD
authors: Rubanza
authors: Michael
authors: M
authors: Wink
mesh_terms: D018922:HL-60 Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D016488:Medicine, African Traditional; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013636:Tanzania; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D014344:Trypanocidal Agents