title: Rapid activation of specific phospholipase(s) D by cytokinin in Amaranthus assay system.
abstract: The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using the level of phosphatidylbutanol production as a measure of PLD activity. Rapid activation of phosphatidylbutanol synthesis was demonstrated as early as within 5 min of cytokinin administration. Neomycin, a known phosphatidylinositol-4,5-bisphosphate (PIP(2)) antagonist, strongly repressed both physiological cytokinin effect and cytokinin-dependent PLD activation. N-acylethanolamine (NAE 12), an inhibitor of alpha-class PLD, did not influence significantly cytokinin effect on Amaranthus seedlings. Together, results suggest the involvement of PIP(2)-dependent non-class alpha-PLD in the molecular mechanism of cytokinin action.
authors: The Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Murmanska 1, Kiev 02094, Ukraine.
authors: Volodymir S
authors: VS
authors: Kravets
authors: Yaroslav S
authors: YS
authors: Kolesnikov
authors: Sergey V
authors: SV
authors: Kretynin
authors: Irina A
authors: IA
authors: Getman
authors: Georgy A
authors: GA
authors: Romanov
mesh_terms: D027721:Amaranthus / Q000201:enzymology*; D003583:Cytokinins / Q000494:pharmacology*; D004983:Ethanolamines / Q000494:pharmacology; D020404:Glycerophospholipids / Q000096:biosynthesis; D009355:Neomycin / Q000494:pharmacology; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000037:antagonists & inhibitors; D010739:Phospholipase D / Q000378:metabolism*; D037121:Plant Lectins / Q000096:biosynthesis; D054788:Ribosome Inactivating Proteins / Q000096:biosynthesis; D054789:Ribosome Inactivating Proteins, Type 1; D036226:Seedlings / Q000201:enzymology
chemical_list: D003583:Cytokinins; D004983:Ethanolamines; D020404:Glycerophospholipids; C022203:N-acylethanolamines; D019269:Phosphatidylinositol 4,5-Diphosphate; D037121:Plant Lectins; D054789:Ribosome Inactivating Proteins, Type 1; C061152:amaranthin protein, Amaranthus; C068564:phosphatidylbutanol; D010739:Phospholipase D; D054788:Ribosome Inactivating Proteins; D009355:Neomycin