[ShRNA of Cyclin D1 decreased the proliferation of human osteosarcoma cell line SOSP-9607.].
OBJECTIVE
To investigate the effect of Cyclin D1 shRNA on the apoptosis and proliferation of human osteosarcoma cell line SOSP-9607.


METHODS
Human Cyclin D1 shRNA vector was stably transfected into SOSP-9607 osteosarcoma cells. The mRNA and protein cxpression levels of Cyclin D1 were detected by semiquantitative RT-PCR and Western blot respectively. The cell cycle and pretiferation of osteosarcoma cells were examined by FCM analysis and CCK-8 method, respectively.


RESULTS
After stable transfection of Cyclin D1 shRNA, the expression of Cyclin D1 were inhibited at mRNA and protein levels. The proliferation of SOSP-9607 osteosarcoma cells was inhibited. The difference was significant compared with control groups (P<0.05). At the same time, Cyclin D1 shRNA transfection increased G0/1 phage content and decreased S phage content.


CONCLUSIONS
Cyclin D1 shRNA could down-regulate the expression of Cyclin D1, effectively inhibit the proliferation of osteosarcoma cells, and have significant effect on the cell cycle.
Ding
Yong
Y
Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Fan
De-Gang
DG
Shan
Le-Qun
LQ
Wang
Yu-Cai
YC
Yang
Tong-Tao
TT
Ma
Bao-An
BA
D001859:Bone Neoplasms; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D012516:Osteosarcoma; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D034741:RNA, Small Interfering; D019938:Cyclin D1