title: Airway responsiveness and bronchoalveolar lavage fluid profiling in individual rats: effects of different ovalbumin exposures.
abstract: We studied repeatedly the development of bronchial hyperreactivity (BHR) and bronchoalveolar lavage fluid (BALF) in rats undergoing different modes of ovalbumin exposures. Treatment was two intraperitoneal injections of ovalbumin in Groups 1-3, followed by one ovalbumin aerosolization in Groups 2 and 3, while rats in Group 4 received repeated ovalbumin aerosols after one single intraperitoneal injection. BHR was assessed longitudinally on day 0 (before treatment) and on day 14 (Groups 1 and 2) or 20 (Groups 3 and 4) and cellular influx was estimated from BALF. No BHR or change in BALF cellular profile was detected in Groups 1-3. However, the infiltration of inflammatory cells, associated with BHR (PC(100) 8.9+/-1.3 microg/kg vs. 4.2+/-1.1 microg/kg), was observed in Group 4. The BHR was always associated with increased number of eosinophils in the BALF. The substantial interindividual variability confirmed the need for a technique that permits follow-up of lung responsiveness and BALF profile. This approach evidenced strong associations between the severity of BHR and the eosinophilia.
authors: Department of Medical Informatics and Engineering, University of Szeged, Szeged, Hungary. petak@dmi.szote.u-szeged.hu
authors: Ferenc
authors: F
authors: Peták
authors: Andrea
authors: A
authors: Bánfi
authors: Valéria
authors: V
authors: Tóth-Szüki
authors: Levente
authors: L
authors: Baráti
authors: Ferenc
authors: F
authors: Bari
authors: Edgár
authors: E
authors: Székely
authors: Zoltán
authors: Z
authors: Novák
mesh_terms: D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000276:immunology* / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid; D016085:Bronchoconstrictor Agents / Q000494:pharmacology / Q000627:therapeutic use; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects; D016210:Methacholine Chloride / Q000494:pharmacology / Q000627:therapeutic use; D009504:Neutrophils / Q000187:drug effects; D010047:Ovalbumin / Q000145:classification / Q000276:immunology* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics / Q000502:physiology*; D013997:Time Factors
chemical_list: D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride; D010047:Ovalbumin