A turn-on fluorescent sensor for detecting nickel in living cells.
We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to Ni(2+) in aqueous solution and in living cells. NS1 combines a BODIPY chromophore and a mixed N/O/S receptor to provide good selectivity for Ni(2+) over a range of biologically abundant metal ions in aqueous solution. In addition to these characteristics, confocal microscopy experiments further show that NS1 can be delivered into living cells and report changes in intracellular Ni(2+) levels in a respiratory cell model.
Dodani
Sheel C
SC
Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
He
Qiwen
Q
Chang
Christopher J
CJ
D000085:Acetates / Q000138:chemical synthesis* / Q000737:chemistry; D001896:Boron Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D009532:Nickel / Q000032:analysis*; D013050:Spectrometry, Fluorescence
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000085:Acetates; D001896:Boron Compounds; D005456:Fluorescent Dyes; D009532:Nickel