The effect of poly (ethylene-co-vinyl alcohol) on senescence-associated alterations of human dermal fibroblasts.
It is well known that biomaterials play an important role in the regulation of adhesion and growth of a variety of cultured cell types. However, whether biomaterials are associated with the senescence of cultured cells is not known. The present work shows that the decrease of the hydrophobic property of poly (ethylene-co-vinyl alcohol) (EVAL) from 44 mole% to 27 mole% ethylene could induce characteristic senescence-associated phenotypic changes such as larger cell shape, re-organized actin cytoskeleton, lower proliferation capacity, higher levels of senescence-associated beta-galactosidase (SA beta-gal) activity, and upregulation of the cell-cycle inhibitor p53 and its transcriptional target p21 in the cultured human diploid fibroblasts (HDFs). Furthermore, it was found that the cultured cells recovered their ability to grow when the substrate was reused every passage. It seemed that the extracellular matrix (ECM) proteins adsorbed onto the EVAL surface might have a protective role in the cellular aging process. Therefore, whether a biomaterial strongly influences cellular aging process must be considered in the selection of a biomaterial for the biomedical application.
Lou
Pei-Jen
PJ
Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 100, Taiwan.
Chiu
Ming-Yi
MY
Chou
Chi-Chun
CC
Liao
Bor-Wu
BW
Young
Tai-Horng
TH
D000199:Actins / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D003247:Conservation of Natural Resources; D003470:Culture Media; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D057075:Enzyme Assays; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D022001:Focal Adhesions / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008567:Membranes, Artificial; D011145:Polyvinyls / Q000494:pharmacology*; D022502:Stress Fibers / Q000187:drug effects / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000199:Actins; D001672:Biocompatible Materials; D003470:Culture Media; D050759:Cyclin-Dependent Kinase Inhibitor p21; D008567:Membranes, Artificial; D011145:Polyvinyls; D016159:Tumor Suppressor Protein p53; C020320:ethylene-vinyl alcohol copolymer; D007770:L-Lactate Dehydrogenase; D001616:beta-Galactosidase