title: Voltage-dependent Ca Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach.
abstract: The properties of voltage dependent Ca(2+) current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min(-1), respectively, in the presence of 1 microM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K(+) -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs(+)-rich pipette solution at V(h)=-80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1 microM) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca(2+) currents (VDCC(L)).
authors: Department of Physiology, Chungbuk National University, College of Medicine, Cheongju 361-763, Korea.
authors: Young Chul
authors: YC
authors: Kim
authors: Hikaru
authors: H
authors: Suzuki
authors: Wen-Xie
authors: WX
authors: Xu
authors: Hikaru
authors: H
authors: Hashitani
authors: Woong
authors: W
authors: Choi
authors: Hyo-Yung
authors: HY
authors: Yun
authors: Seon-Mee
authors: SM
authors: Park
authors: Sei Jin
authors: SJ
authors: Youn
authors: Sang-Jeon
authors: SJ
authors: Lee
authors: Sang Jin
authors: SJ
authors: Lee