Assembly, tuning and use of an apertureless near field infrared microscope for protein imaging.
This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented.
Paulite
Melissa
M
Department of Chemistry, University of Toronto, Toronto, Canada.
Fakhraai
Zahra
Z
Akhremitchev
Boris B
BB
Mueller
Kerstin
K
Walker
Gilbert C
GC
D000682:Amyloid / Q000737:chemistry; D007259:Infrared Rays; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
D000682:Amyloid; D011506:Proteins