Melittin-regenerated purple membrane.
We have investigated the character of melittin-regenerated purple membrane. Adding melittin to blue membrane causes the color transition and partial regeneration of the photocycle and the proton pump. The reconstitution of bacteriorhodopsin by melittin is proved to be charge-dependent. In studying the location of melittin binding on the blue membrane, we suggest that melittin anchors on the membrane through both hydrophobic and electrostatic interactions. The electrostatic interaction is dominant. The binding sites for the electrostatic interaction should be on the surface of the membrane.
Zhang
Yue
Y
Institute of Biophysics, Academia Sinica, Beijing, 100101, PR China.
Su
Tao
T
Hu
Kun-Sheng
KS
D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000737:chemistry; D005453:Fluorescence; D057927:Hydrophobic and Hydrophilic Interactions; D008555:Melitten / Q000737:chemistry*; D008969:Molecular Sequence Data; D018717:Purple Membrane / Q000737:chemistry*; D055672:Static Electricity
D008555:Melitten; D001436:Bacteriorhodopsins