Dynamin- and clathrin-dependent endocytosis in African swine fever virus entry.
African swine fever virus (ASFV) is a large DNA virus that enters host cells after receptor-mediated endocytosis and depends on acidic cellular compartments for productive infection. The exact cellular mechanism, however, is largely unknown. In order to dissect ASFV entry, we have analyzed the major endocytic routes using specific inhibitors and dominant negative mutants and analyzed the consequences for ASFV entry into host cells. Our results indicate that ASFV entry into host cells takes place by clathrin-mediated endocytosis which requires dynamin GTPase activity. Also, the clathrin-coated pit component Eps15 was identified as a relevant cellular factor during infection. The presence of cholesterol in cellular membranes, but not lipid rafts or caveolae, was found to be essential for a productive ASFV infection. In contrast, inhibitors of the Na(+)/H(+) ion channels and actin polymerization inhibition did not significantly modify ASFV infection, suggesting that macropinocytosis does not represent the main entry route for ASFV. These results suggest a dynamin-dependent and clathrin-mediated endocytic pathway of ASFV entry for the cell types and viral strains analyzed.
Hernaez
Bruno
B
Departamento de Biotecnologia-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Autovia A6 km 7, 28040 Madrid, Spain.
Alonso
Covadonga
C
D000199:Actins / Q000037:antagonists & inhibitors; D000358:African Swine Fever Virus / Q000187:drug effects / Q000472:pathogenicity* / Q000502:physiology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D021941:Caveolae / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002522:Chlorocebus aethiops; D002746:Chlorpromazine / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism; D002966:Clathrin / Q000502:physiology*; D034281:Dynamins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000037:antagonists & inhibitors; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014162:Transfection; D014168:Transferrin / Q000378:metabolism; D014709:Vero Cells; D053586:Virus Internalization / Q000187:drug effects; D047392:beta-Cyclodextrins / Q000494:pharmacology
D000199:Actins; D002966:Clathrin; D007473:Ion Channels; D011993:Recombinant Fusion Proteins; D014168:Transferrin; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol; D034281:Dynamins; D002746:Chlorpromazine