title: The presence of mature granulocytes/monocytes derived from leukemic cells in MLL-associated leukemia.
abstract: We observed the mature granulocytes/monocytes derived from leukemic cells in patients with acute myeloid leukemia who present mixed lineage leukemia gene (MLL). Morphologic observation and fluorescence in situ hybridization analysis (FISH) for chromosome 11q23 abnormality were studied, and a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was done to identify the fusion partners with MLL. The bone marrow cells with FISH signals of MLL showed the cell differentiation of the myeloid and/or monocytic lineages in 4 of 6 AML patients. MLL partner genes were AF6, AF9, ELL, and ENL, respectively. There was no correlation between the fusion partner and the appearance of mature cells derived from MLL clones. RT-PCR showed the fusion between MLL exon 9 or 10 and the partner genes in mature granulocytes/monocytes. These findings suggest that subgroup of leukemia cells with MLL rearrangement has the differentiation potential of leukemic cells and mature granulocytes/monocytes derived from MLL clones may be biologically different from normal mature cells.
authors: Division of Clinical Laboratory, Kanagawa Cancer Center, Yokohama, Japan.
authors: Shoichi
authors: S
authors: Kobayashi
authors: Division of Clinical Laboratory, Kanagawa Cancer Center, Yokohama, Japan.
authors: Masato
authors: M
authors: Obata
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Maki
authors: M
authors: Hagihara
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Kenji
authors: K
authors: Motohashi
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Satomi
authors: S
authors: Ito
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Rika
authors: R
authors: Ohshima
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Rika
authors: R
authors: Sakai
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan.
authors: Atsuo
authors: A
authors: Maruta
authors: Department of Hematology, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama, 241-0815, Japan. kanamori@kcch.jp.
authors: Heiwa
authors: H
authors: Kanamori
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000473:pathology; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*
chemical_list: D051788:Myeloid-Lymphoid Leukemia Protein