Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Recently, we established and phenotypically characterized an immortalized porcine olfactory bulb neuroblast cell line, OBGF400 (1). To facilitate the future application of these cells in studies of neurological dysfunctions and neuronal pathogen interactions, a comprehensive knowledge of their genomic variability and overall gene expression capacity was pursued. Accordingly, the OBGF400 cells were subjected to karyotyping and more extensive transcriptome analyses. Cytogenetic characterization of these cells revealed a genetic mosaicism of neuronal hyperdiploidy. A direct comparison of the OBGF400 cell transcriptome pattern, generated by utilizing the Affymetrix GeneChip(R) Porcine Genome Array, to that of a non-neural, porcine epithelial cell line facilitated the identification of 831 probe sets preferentially hybridized by the neuroblast transcripts. Subsequent functional annotation of these OBGF400 RNAs using the Database for Annotation, Visualization and Integrated Discovery 2008 enabled their allocation to the corresponding gene ontology biological process term, thereby assisting the recognition of key elements involved in the regulation of neuronal signal transduction and neurogenesis.
Uebing-Czipura
A U
AU
Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
Dawson
H D
HD
Rutherford
M S
MS
Scherba
G
G
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D020869:Gene Expression Profiling / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D055495:Neurogenesis; D009830:Olfactory Bulb / Q000737:chemistry* / Q000166:cytology; D015398:Signal Transduction; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D034421:Sus scrofa