title: Target chemotherapy of anti-CD147 antibody-labeled liposome encapsulated GSH-DXR conjugate on CD147 highly expressed carcinoma cells.
abstract: It was confirmed that CD147 (Emmprin) was expressed on the cell surface of carcinoma cells. For the purpose of studying the efficacy of a CD147-targeting agent on CD147-expressing carcinoma cells, we investigated the effect of a conjugate of glutathione-doxorubicin (GSH-DXR) encapsulated in an anti-CD147 antibody-labeled liposome (aCD147ab-liposome) in terms of specific accumulation and cytotoxicity in CD147-expressing human carcinoma cells. Expression of CD147 was not observed in many normal human tissues. However, slight expression of CD147 in kidney, prostate and breast tissues was observed. By contrast, high-level expression of CD147 in all carcinoma cells such as A431, PC3 and Ishikawa cell lines was confirmed by fluorescent microscopy and Western blot analysis. Specific accumulation of the aCD147ab-liposome in the above-described CD147-expressing cells was observed. GSH-DXR encapsulated in an aCD147ab-liposome expressed specific cytotoxicity against these carcinoma cells. These results suggested that target chemotherapy of GSH-DXR encapsulated in an aCD147ab-liposome on CD147-expressing carcinoma cells was effective.
authors: Department of Biochemistry, Jikei University School of Medicine, Tokyo 105-8461, Japan. hi-matz48@jikei.ac.jp
authors: Hiroshi
authors: H
authors: Matsudaira
authors: Tadashi
authors: T
authors: Asakura
authors: Katsuhiko
authors: K
authors: Aoki
authors: Yasuyuki
authors: Y
authors: Searashi
authors: Tomokazu
authors: T
authors: Matsuura
authors: Hisato
authors: H
authors: Nakajima
authors: Hisao
authors: H
authors: Tajiri
authors: Kiyoshi
authors: K
authors: Ohkawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000494:pharmacology; D051926:Basigin / Q000737:chemistry*; D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D005978:Glutathione / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D005982:Glutathione Transferase / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D008081:Liposomes; C436625:glutathione-doxorubicin conjugate; D051926:Basigin; D004317:Doxorubicin; D005982:Glutathione Transferase; D005978:Glutathione