Identification of the Salmonella enterica damX gene product, an inner membrane protein involved in bile resistance.
The damX gene product of Salmonella enterica serovar Typhimurium is a protein located in the inner membrane. DamX migrates as a 70-kDa protein in SDS-PAGE even though the predicted protein size is 46 kDa. Synthesis of DamX protein occurs in both exponential- and stationary-phase cultures. Disruption of damX causes severe sensitivity to bile. Lack of the outer membrane protein AsmA suppresses bile sensitivity in Salmonella damX mutants.
López-Garrido
Javier
J
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, E-41080 Seville, Spain.
Cheng
Nancy
N
García-Quintanilla
Fátima
F
García-del Portillo
Francisco
F
Casadesús
Josep
J
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D008565:Membrane Proteins