Effect of hyperglycemia on apoptosis of notochordal cells and intervertebral disc degeneration in diabetic rats.
OBJECTIVE
Diabetes mellitus is thought to be an important etiologic factor in intervertebral disc degeneration. It is known that notochordal cells gradually disappear from the nucleus pulposus (NP) of the intervertebral disc with age by undergoing apoptosis. What is not known is whether diabetes has an effect on apoptotic rates of notochordal cells. The purpose of this study was to investigate the effect of hyperglycemia on apoptosis of notochordal cells and intervertebral disc degeneration in age-matched OLETF (diabetic) and LETO (control) rats.


METHODS
Lumbar disc tissue (L1-2 through L5-6), including cranial and caudal cartilaginous endplates, was obtained from 6- and 12-month-old OLETF and LETO rats (40 rats, 10 in each of the 4 groups). The authors examined the NP using TUNEL, histological analysis, and Western blot for expression of matrix metalloproteinase (MMP)-1, -2, -3, and -13, tissue inhibitor of metalloproteinase (TIMP)-1 and -2, and Fas (apoptosis-related protein). The apoptosis index of notochordal cells was calculated. The degree of transition of notochordal NP to fibrocartilaginous NP was classified on a scale ranging from Grade 0 (no transition) to Grade 4 (transition > 75%). The degree of expression of MMP-1, -2, -3, and -13, TIMP-1 and -2, and Fas was evaluated by densitometry.


RESULTS
At 6 and 12 months of age, OLETF rats showed increased body weight and abnormal 2-hour glucose tolerance tests compared with LETO rats. The apoptosis index of notochordal cells was significantly higher in the OLETF rats than in the LETO rats at both 6 and 12 months of age. The degree of transition of notochordal NP to fibrocartilaginous NP was significantly higher in the OLETF rats than in the LETO rats at 6 and 12 months of age. The expression of MMP-1, -2, -3, and -13, TIMP-1, and Fas was higher in the OLETF rats at 6 and 12 months of age. The expression of TIMP-2 was significantly higher in the OLETF rats than in the LETO rats at 6 months of age, but not at 12.


CONCLUSIONS
The findings suggest that diabetes is associated with premature, excessive apoptosis of NP notochordal cells. This results in an accelerated transition of a notochordal NP to a fibrocartilaginous NP, which leads to early intervertebral disc degeneration. It remains to be determined if these premature changes are due to hyperglycemia or some other factors associated with diabetes. Understanding the mechanism by which diabetes affects disc degeneration is the first step in designing therapeutic modalities to delay or prevent disc degeneration caused by diabetes mellitus.
Won
Ho-Yeon
HY
Department of Orthopaedic Surgery, College of Medicine, Catholic University of Korea, Seoul, Korea.
Park
Jong-Beom
JB
Park
Eun-Young
EY
Riew
K Daniel
KD
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism; D006943:Hyperglycemia / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D007403:Intervertebral Disc / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000473:pathology*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009672:Notochord / Q000166:cytology / Q000473:pathology*; D010784:Photomicrography; D051381:Rats; D020334:Rats, Inbred OLETF; D018709:Statistics, Nonparametric; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism
C505535:Fadd protein, rat; D053200:Fas-Associated Death Domain Protein; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases