Isolation of cutaneous dendritic cells.
Cutaneous dendritic cells represent the first immunological interface with the environment and play a key role in the defense against pathogens that breach the skin. This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies.
Helft
Julie
J
Department of Gene and Cell Medicine and The Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA.
Merad
Miriam
M
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D005434:Flow Cytometry; D007801:Langerhans Cells / Q000166:cytology*; D051379:Mice