title: Use of Hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters.
abstract: Filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-microm-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry.
authors: Office of Biotechnology Products, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA.
authors: A
authors: A
authors: Lee
authors: J
authors: J
authors: McVey
authors: P
authors: P
authors: Faustino
authors: S
authors: S
authors: Lute
authors: N
authors: N
authors: Sweeney
authors: V
authors: V
authors: Pawar
authors: M
authors: M
authors: Khan
authors: K
authors: K
authors: Brorson
authors: D
authors: D
authors: Hussong
mesh_terms: D000393:Air Pollutants; D001419:Bacteria; D001431:Bacteriological Techniques / Q000295:instrumentation; D015169:Colony Count, Microbial; D042621:Comamonadaceae / Q000648:ultrastructure; D003470:Culture Media; D004203:Disinfection / Q000295:instrumentation; D004340:Drug Contamination; D004345:Drug Industry / Q000295:instrumentation; D004784:Environmental Monitoring / Q000295:instrumentation; D005374:Filtration / Q000295:instrumentation*; D005618:Fresh Water; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D013242:Sterilization / Q000295:instrumentation; D014871:Water Microbiology; D018508:Water Purification / Q000295:instrumentation
chemical_list: D000393:Air Pollutants; D003470:Culture Media; D008567:Membranes, Artificial