Genomic sequencing and characterization of a Chinese isolate of Bovine viral diarrhea virus 2.
The complete genomic sequencing and characterization of Bovine viral diarrhea virus (BVDV) isolate XJ-04 originated from cattle in China was described. The genomic RNA of the isolate was 12,284 nt long and contained short 5'- untranslated region (UTR), 3'-non-coding regions (NCR), and one open reading frame (ORF) encoding a large polyprotein of 3,895 amino acids with 20 potential N-glycosylation sites. The identity of the isolate XJ-04 with reference strains NADL (BVDV-1) and 890 (BVDV-2) in autoprotease (N(pro)) gene and structural genes (C, E(rns), E1, E2) was analyzed. The percentage of nt and aa identity in analyzed genes indicated that the isolate XJ-04 was closer to the reference strain 890 (BVDV-2) than to NADL (BVDV-1). Phylogenetic analysis revealed that the isolate belonged to BVDV-2a subtype. Furthermore, comparison analysis indicated that the isolate XJ-04 did not contain any genomic insertions that can be directly related to the cytopathic phenotype.
Zhu
L Q
LQ
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R. China.
Lin
Y Q
YQ
Ding
X Y
XY
Ren
M
M
Tao
J
J
Wang
J Y
JY
Zhang
G P
GP
Zhu
G Q
GQ
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry
D020121:5' Untranslated Regions; D012367:RNA, Viral