Gene transcription of TLR2, TLR4, LPS ligands and prostaglandin synthesis enzymes are up-regulated in canine uteri with cystic endometrial hyperplasia-pyometra complex.
Escherichia coli (E. coli) is the most frequent bacterium isolated in cases of cystic endometrial hyperplasia-pyometra complex, the most frequent endometrial disorder in the bitch. Toll-like receptors (TLRs) play an essential role in the innate immune system. The aim of this study was to compare transcription of genes encoding TLR2, TLR4 and LPS ligands (CD14, MD-2, LBP), prostaglandin synthesis enzymes (COX1, COX2, PGES1 and PGFS), and to compare COX1 and COX2 protein expression and PGE(2) and PGF(2alpha) endometrial content in the endometrium of canine diestrous uteri with (n=7) or without (n=7) pyometra. All cases of pyometra were hyperplastic and E. coli was the only isolated bacteria, while diestrous normal uteri did not present signs of cystic endometrial hyperplasia and were negative for bacteriology. Except for COX1, transcription of all genes was significantly higher in pyometra than in normal endometria. COX1 protein was observed in both normal and pyometra uteri, but COX2 protein was only present in pyometra cases. Endometrial PGE(2) and PGF(2alpha) content were significantly higher in pyometra than in normal diestrous endometria. In conclusion, data obtained in this study provides evidence that pyometra-isolated E. coli induces the up-regulation of TLR2 and TLR4 genes in the canine diestrous endometrium. This up-regulation, which is probably the result of the stimulation by LPS and lipoprotein E. coli constituents, leads to the endometrial up-regulation of PG synthesis genes. This, in turn, results in a higher endometrial concentration of PGE(2) and PGF(2alpha), which may further regulate the local inflammatory response.
Silva
E
E
Interdisciplinary Centre of Research in Animal Health, Faculty of Veterinary Medicine, TULisbon, Lisboa, Portugal.
Leitão
S
S
Henriques
S
S
Kowalewski
M P
MP
Hoffmann
B
B
Ferreira-Dias
G
G
da Costa
L Lopes
LL
Mateus
L
L
D000209:Acute-Phase Proteins / Q000235:genetics; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D051545:Cyclooxygenase 1 / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004283:Dog Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D004285:Dogs; D004714:Endometrial Hyperplasia / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000235:genetics / Q000662:veterinary*; D005260:Female; D005786:Gene Expression Regulation; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008070:Lipopolysaccharides / Q000276:immunology; D051218:Lymphocyte Antigen 96; D008562:Membrane Glycoproteins / Q000235:genetics; D055112:Pyometra / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D051195:Toll-Like Receptor 2 / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014158:Transcription, Genetic; D015854:Up-Regulation; D014599:Uterus / Q000276:immunology* / Q000382:microbiology; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015237:Dinoprost; D015232:Dinoprostone; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051218:Lymphocyte Antigen 96; D008562:Membrane Glycoproteins; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D000099231:Lipopolysaccharide-Binding Protein