title: Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11).
abstract: This study was conducted to investigate the amount of CLA synthesized endogenously by rat mammary tissues in response to TVA (a precursor for cis-9, trans-11 CLA endogenous synthesis) treatment as well as the differences in the protein expression of genes encoding the biosynthesis of CLA in rat mammary tissue and mouse mammary gland epithelia cells (HC11). Treatment with TVA resulted in improved CLA productivity. Furthermore, 2-DE revealed two spots in samples of mammary tissues and one spot in samples of mammary gland epithelia cells (HC11) that were consistently altered in the TVA treatment groups when compared with the control group (non-fatty acid). The mRNA expression patterns of three of the proteins (PDI, PRDX2, LAMR1), as measured by real-time PCR, were similar to the pattern of protein abundance. In addition, the expression of SCD mRNA in the mammary tissue of rats and HC11 cell treated with TVA was higher than in the control group. Our results suggest that the identified proteins may be related to CLA biosynthesis in mammary tissue.
authors: Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 157-921, Republic of Korea.
authors: Yong-Cheng
authors: YC
authors: Jin
authors: Hong-Gu
authors: HG
authors: Lee
authors: Cheng-Xiong
authors: CX
authors: Xu
authors: Jeng-A
authors: JA
authors: Han
authors: Seong-Ho
authors: SH
authors: Choi
authors: Man-Kang
authors: MK
authors: Song
authors: Young-Jun
authors: YJ
authors: Kim
authors: Ki-Beom
authors: KB
authors: Lee
authors: Seon-Ku
authors: SK
authors: Kim
authors: Han-Seok
authors: HS
authors: Kang
authors: Byung-Wook
authors: BW
authors: Cho
authors: Teak-Soon
authors: TS
authors: Shin
authors: Yun-Jaie
authors: YJ
authors: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007774:Lactation / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009829:Oleic Acids / Q000494:pharmacology; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D012333:RNA, Messenger; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; C086117:Rpsa protein, rat; D054464:Peroxiredoxins; D019704:Protein Disulfide-Isomerases; C050413:11-octadecenoic acid