Peptide and protein quantification: a map of the minefield.
The increasing popularity of gel-free proteomics technologies has created a strong demand for compatible quantitative analysis methods. As a result, a plethora of different techniques has been proposed to perform gel-free quantitative analysis of proteomics samples. Each of these methods comes with certain strengths and shortcomings, and they often are dedicated to a specific purpose. This review will present a brief overview of the main methods, organized by their underlying concepts, and will discuss the issues they raise with a focus on data processing. Finally, we will list the available software that can help with the data processing from quantitative experiments. We hope that this review will thus enable researchers to find the most appropriate method available for their research objectives, and can also serve as a basis for creating a reliable data processing strategy.
Vaudel
Marc
M
ISAS - Institute for Analytical Sciences, Dortmund, Germany. marc.vaudel@isas.de
Sickmann
Albert
A
Martens
Lennart
L
D000818:Animals; D030562:Databases, Protein; D001330:Electronic Data Processing / Q000379:methods; D006801:Humans; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D012984:Software
D010455:Peptides; D011506:Proteins