[Study on the relationship between hepatocellular carcinoma and the interaction between polymorphisms in DNA repair gene XPD and environmental factors].
OBJECTIVE
To study the relationship between hepatocellular carcinoma and the interaction of polymorphisms in DNA repair gene XPD with environmental factors.


METHODS
A hospital-based case-control study on hepatocellular carcinoma was conducted. All the hepatocellular carcinoma cases (n=300) were newly diagnosed and controls (n=312) were diagnosed with non-tumor cases. XPD genotype (Lys751 Gln and Asp312 Asn) from blood derived DNA was determined using TaqMan MGB Real-time PCR. Unconditional logistic regression was used to estimate the odds ratios (ORs) and 95% confidence intervals (CIs).


RESULTS
For XPD condon 751 genotypes, there was no significant difference between frequencies of the AC or CC among patients and controls (P>0.05) (referent AA). The frequency of XPD312A allelic gene was higher in cases than that in controls and was associated with an increased risk (adjusted OR=2.62, 95% CI: 1.626-4.222) for hepatocellular carcinoma when compared with GG genotype. Interactions were found between infection of HBsAg and XPD312 (OR=7.348), as well as between smoking and non-wild type gene of XPD751 (OR=4.291) and XPD312 (OR=5.341).


CONCLUSIONS
DNA repair XPD312A allelic gene might increase the risk of Hepatocellular carcinoma. Interactions between HBsAg infection, smoking and XPD were observed in Hepatocellular carcinoma.
Zeng
Xiao-Yun
XY
Public Health School, Guangxi Medical University, Nanning, China.
Qiu
Xiao-Qiang
XQ
Ji
Long
L
Yu
Hong-Ping
HP
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006509:Hepatitis B / Q000150:complications; D006514:Hepatitis B Surface Antigens; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D016015:Logistic Models; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human