title: Conjugative transposition of Tn916 and detection of Tn916-Like transposon in Erysipelothrix rhusiopathiae.
abstract: In order to investigate the origin of tetracycline resistance in Erysipelothrix rhusiopathiae, conjugative transpositions of Tn916 were tested. The frequency of transfer between strains of E. rhusiopathiae was about 10-fold higher than that between Enterococcus faecalis and E. rhusiopathiae. In addition, detection of a Tn916-like transposon was performed by PCR assay and DNA sequencing in E. rhusiopathiae field isolates. Of 49 tetracycline-resistant isolates, 38 (77.6 %) carried a Tn916-like transposon, while 11 (22.4 %) carried tet(M) only. These results suggested that Tn916-like transposon may be widely present in the E. rhsuiopathiae field isolates resistant to tetracycline.
authors: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo, Japan. ozawa@nval.go.jp
authors: Manao
authors: M
authors: OZAWA
authors: Kinya
authors: K
authors: YAMAMOTO
authors: Akemi
authors: A
authors: KOJIMA
authors: Masami
authors: M
authors: TAKAGI
authors: Toshio
authors: T
authors: TAKAHASHI
mesh_terms: D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004888:Erysipelothrix / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D004251:DNA Transposable Elements; D013752:Tetracycline