The interaction of Glu294 at the subunit interface is important for the activity and stability of goose delta-crystallin.
OBJECTIVE
delta-Crystallin is a soluble structural protein in found in avian eye lenses; it shares high amino acid sequence identity with argininosuccinate lyase. E294 is the only residue located at the double dimer interface and it performs hydrogen bonding with the active site residues of H160 and K323 in the neighboring and diagonal subunits, respectively. H160 is reported to play an important role in catalysis due to its H-bond interaction with the fumarate moiety of the substrate. In order to clarify the function of E294 in either stabilization of the quaternary structure or in catalysis, we carried out site-directed mutagenesis and functional analysis.


METHODS
The structure of both wild-type and mutant proteins were analyzed by circular dichroism (CD) spectroscopy, fluorescence spectra, and analytical ultracentrifugation. Structural stability was measured by CD and tryptophan fluorescence. A modeled structure of the E294L mutant was built and optimized with energy minimization.


RESULTS
No gross structural changes were observed when E294 was substituted with leucine, as judged by circular dichroism, tryptophan fluorescence, ANS fluorescence, and sedimentation velocity analyses. However, this mutant enzyme had only about 10% of the activity of a wild-type enzyme and its secondary structure was more easily denatured by increased temperature than that of a wild-type enzyme. The mutant protein also underwent its first unfolding transition at a lower concentration of guanidinium-hydrochloride than the wild-type protein.


CONCLUSIONS
These results indicate that the interactions offered by E294 in the dimer-dimer interface of delta-crystallin are required to maintain the hydrogen bonding network in the active site for catalysis. Disruption of the interaction had no significant effect on the conformation and quaternary structure of delta-crystallin but it did lead to instability in the double dimer structure.
Huang
Chih-Wei
CW
Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan.
Chen
Yu-Hou
YH
Chen
Ya-Huei
YH
Tsai
Yun-Chi
YC
Lee
Hwei-Jen
HJ
D000595:Amino Acid Sequence; D000818:Animals; D005777:Geese / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D019791:Guanidine / Q000494:pharmacology; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D038207:delta-Crystallins / Q000737:chemistry* / Q000378:metabolism*
D050505:Mutant Proteins; D021122:Protein Subunits; D038207:delta-Crystallins; D018698:Glutamic Acid; D019791:Guanidine