title: [Considering the role of the PCR method in routine diagnosis of toxoplasmosis in peripheral blood tests in immunocompetent patients].
abstract: Infections caused by pathogenic protozoan Toxoplasma gondii in our geographic area is the most frequent parasitic infection; Czech Republic declares seroprevalence approx. 30 %. Diagnosis of toxoplasmosis is mostly based on serological methods are used (EIA IgM, IgA, IgE, IgG and avidity in IgG, Western Blot, complement fixation). According to positive results of these tests diagnosis of acute toxoplasmosis is established. In our retrospective study we tried to evaluate results of T. gondii DNA positivity from blood samples by PCR compared with positive markers of acute infection in patients before specific therapy was initiated. In accordance with literature we concluded, that in routine examination of immunocompetent outpatients of Clinic of Infectious Diseases from the moment of 4 weeks after lymphatic nodes swelling protozoan DNA detection in blood sample is not possible.
authors: Department of Clinical Microbiology, University Hospital Hradec Králové, Charles University, Czech Republic. cermakovaz@fnhk.cz
authors: Zuzana
authors: Z
authors: Cermáková
authors: Petr
authors: P
authors: Prásil
authors: Zbynek
authors: Z
authors: Valenta
authors: Miroslav
authors: M
authors: Förstl
authors: Lenka
authors: L
authors: Plísková
authors: Radka
authors: R
authors: Bolehovská
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003168:Complement Fixation Tests; D016054:DNA, Protozoan / Q000097:blood*; D005260:Female; D006801:Humans; D007121:Immunocompetence; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D007136:Immunoglobulins