Characterization of tomato yellow vein streak virus, a begomovirus from Brazil.
Tomato yellow vein streak virus (ToYVSV) is a tentative begomovirus (Family Geminiviridae) species that seriously affects tomato and potato production in Brazil. Here, we have determined the genomic and biological characteristics of a ToYVSV isolate (Ba3) from a potato plant sampled in Rio Grande do Sul State, Brazil. The DNA-A nucleotide sequence of Ba3 and another previously reported ToYVSV isolate share 89.7% sequence identity. These ToYVSV isolates should be classified as a new species in that they are most closely related to Soybean blistering mosaic virus with which they share only approximately 80% identity. Cloned constructs containing 1.5 mer copies of the ToYVSV genomic components were found, by biolistic bombardment, to be infectious in at least 11 plant species in 2 families (Solanaceae and Malvaceae). Symptoms on tomato and potato plants were identical to those originally observed on field-infected plants. ToYVSV was also sap-transmissible from Nicotiana benthamiana to N. benthamiana and tomato, but not to potato plants.
Albuquerque
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LC
Departament of Plant Pathology, University of Brasília, Brasília, Brazil.
Martin
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DP
Avila
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AC
Inoue-Nagata
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AK
D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D001938:Brazil; D018745:Genome, Plant; D018551:Solanum lycopersicum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D011198:Solanum tuberosum / Q000821:virology