Role of AKT kinase in measles virus replication.
Many RNA and DNA viruses activate serine-threonine kinase AKT to increase virus replication. In contrast, measles virus (MV) infection leads to downregulation of AKT. This is thought to be beneficial for the virus because it correlates with immune suppression. To determine whether this is a sacrifice for the virus, we used a recombinant virus and transfected cells expressing constitutively active AKT and evaluated its effect on virus replication. In vitro, overexpression of AKT did not influence virus replication but did affect (cell-type dependent) virus release. In vivo, the recombinant virus did not abrogate inhibition of proliferation of spleen cells from MV-infected cotton rats.
Carsillo
Mary
M
Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio 43210, USA.
Kim
Dhohyung
D
Niewiesk
Stefan
S
D000818:Animals; D002522:Chlorocebus aethiops; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D008457:Measles / Q000473:pathology / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D016205:Sigmodontinae; D013154:Spleen / Q000473:pathology / Q000821:virology; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
D011994:Recombinant Proteins; D051057:Proto-Oncogene Proteins c-akt