[Chemical constituents and bioactivity of Teucrium pilosum].
OBJECTIVE
To study the chemical constituents and bioactivity of Teurium pilosum.


METHODS
Various column chromatographic techniques were used to isolate the constituents. A combination of EI-MS, NMR spectroscopy and X-Ray were applied to identify the structures. The anti-microorganism was accomplished by disk diffusion method, the antioxidant activity was assayed by the DPPH microanalysis models and the inhibitory activity of alpha-glucosidase was screened In vitro.


RESULTS
Eight compounds were isolated and identified as: glyceryl tristearate (1), 2,5-dioxolanone (2), fernenol (3), stigmasta-5,22-dien-3P-ol (4), 24-nor cholesta-5,22 (E)-dien-3beta-ol (5), ca-spinasterol (6), (3,4-dihydroxyphenyl) acrylate (7), 3,4-dihydroxy phenyl acrylic acid (8).


CONCLUSIONS
All compounds have been isolated from the genus for the first time. Compound 3 [IC50 = (37.63 +/- 3.45) mg +/- L(-1)], 6 [IC50 = (178.92 +/- 4.99) mg x L(-1)] and 8 [IC50 = (44.32 +/- 7.02) mg x L(-1)] are of higher inhibitory alpha-glucosidase activity than that of acarbose [IC50 = (1081.27 +/- 12.3) mg x L(-1)]. Compound7 [IC50 = (4.81 +/- 0.96) mg x L(-1)] and 8 [IC50 = (4.16 +/- 0.11) mg L(-1)] showed higher antioxidant activity than that of BHT [IC50 = (35.64 +/- 0.36) mg x L(-1)] and BHA [IC50 = (8.74 +/- 0.39) mg x L(-1)]. Compound 5-8 exhibited inhibitory activity against Fusarium graminearum. Compound 5 and 8 showed inhibitory activity against Botrytis cinerea.
Mou
Mingyue
M
School of Chemistry & Chemical Engineering of Guizhou University, Guiyang 550025, China.
Zhang
Qianjun
Q
Kang
Wenyi
W
Pi
Ke
K
Chen
Qing
Q
Yao
Rongjun
R
D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005670:Fusarium / Q000187:drug effects; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D031367:Teucrium / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000032:analysis
D000935:Antifungal Agents; D000975:Antioxidants; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000520:alpha-Glucosidases