The contribution of systems biology and reverse genetics to the understanding of Kaposi's sarcoma-associated herpesvirus pathogenesis in endothelial cells.
Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 is the causative agent of the endothelial cell-derived tumour Kaposi's sarcoma. Herpesviruses possess large complex genomes which provide many options to regulate cellular physiology during the viral life cycle and in the course of tumourigenicity. Novel techniques of systems biology and reverse genetics are increasingly applied to dissect the complex interaction of KSHV with endothelial cells. This review will outline novel results and pitfalls of these technologies in the elucidation of KSHV pathogenicity.
Stürzl
Michael
M
Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany. michael.stuerzl@uk-erlangen.de
Konrad
Andreas
A
Alkharsah
Khaled R
KR
Jochmann
Ramona
R
Thurau
Mathias
M
Marquardt
Gaby
G
Schulz
Thomas F
TF
D000818:Animals; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000821:virology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000209:etiology / Q000235:genetics / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D040901:Proteomics; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000235:genetics / Q000821:virology; D015398:Signal Transduction; D049490:Systems Biology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins