[The effect of murine mesenchymal stem cells on the differentiation and function of allogenetic bone marrow-derived dendritic cells.].
OBJECTIVE
To study the effect of murine mesenchymal stem cells (MSCs) on the differentiation, maturation and function of allogenetic bone marrow-derived dendritic cells (DCs) and to investigate the mechanism of MSCs displaying immunoregulatory activity.


METHODS
BALB/c mice BMCs were isolated and cultured in vitro and then coclutured with C57BL/6 murine bone marrows cells (BMCs) at different ratios in vitro to induce DCs generation. The phenotype of cells was analyzed by flow cytometry. Endocytosis was measured as the cellular uptake of fluorescein isothiocyanate (FITC)-dextram amd was quantified by flow cytometry. The level of IL-12 secretion in the cell culture supernatants was detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Decreased expression of CD11c, CD14, CD83, CD86 and I-A(b);, down-regulated endocytosis capacity and interleukin-12 (IL-12) secretion of DCs were all observed when MSCs cocultured with BMCs at a higher ratio (1:10).


CONCLUSIONS
Murine MSCs could supress the differentiation and maturation of DCs derived from allogeneic BMCs in vitro.
Deng
Chun-Yan
CY
Clinical Medical Research Center, Shenzhen People's Hospital, The Second Clinic Medical College of Jinan University, Shenzhen 518020, China.
Li
Fu-Rong
FR
Wang
Xin-Gen
XG
Qi
Hui
H
Wen
Jin-Li
JL
Zhou
Ya-Ying
YY
D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL