title: Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes.
abstract: Keratinocyte differentiation plays a pivotal role in the epidermal barrier. Single keratinocyte differentiation genes have already been studied, but many important constituents of this process may have been missed so far. Gene expression profiling by microarray was carried out in cultured normal human epidermal keratinocytes undergoing confluence-induced differentiation to find novel differentiation genes. Candidate gene lists were established and genes of potential dermatological interest were validated by quantitative reverse transcription polymerase chain reaction and immunohistochemical analysis. Some of these points lead to the identification of counter-regulation of heme oxygenase and biliverdin reductase as well as glutaredoxin and glutathione reductase indicative of potential novel redox signaling in differentiating human keratinocytes. Others indicate a strong concert down-regulation of interleukin-1 signaling at previously unidentified levels during keratinocyte differentiation. We believe that identified genes contribute to a more comprehensive understanding of the complicated epidermal differentiation process and lead to better understanding of dermatological diseases.
authors: György
authors: G
authors: Paragh
authors: Peter
authors: P
authors: Ugocsai
authors: Thomas
authors: T
authors: Vogt
authors: Petra
authors: P
authors: Schling
authors: Alexander E
authors: AE
authors: Kel
authors: Victoria
authors: V
authors: Tarabin
authors: Gerhard
authors: G
authors: Liebisch
authors: Evelyn
authors: E
authors: Orsó
authors: Loránt
authors: L
authors: Markó
authors: Attila
authors: A
authors: Balogh
authors: Tamás
authors: T
authors: Köbling
authors: Eva
authors: E
authors: Remenyik
authors: Norbert M
authors: NM
authors: Wikonkál
authors: József
authors: J
authors: Mandl
authors: Mike
authors: M
authors: Farwick
authors: Gerd
authors: G
authors: Schmitz
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D015894:Genome, Human; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis