title: Altered regulation of glutamate release and decreased functional activity and expression of GLT1 and GLAST glutamate transporters in the hippocampus of adolescent rats perinatally exposed to Delta(9)-THC.
abstract: The long-term effects of perinatal Delta(9)-tetrahydrocannabinol (Delta(9)-THC) exposure - from gestational day (GD) 15 to postnatal day (PND) 9 - on hippocampal glutamatergic neurotransmission were studied in slices from the 40-day-old offspring of Delta(9)-THC exposed (Delta(9)-THC-rats) and vehicle-exposed (control) dams. Basal and in K+-evoked endogenous hippocampal glutamate outflow were both significantly decreased in Delta(9)-THC-rats. The effect of short Delta(9)-THC exposure (0.1microM) on K(+)-evoked glutamate release disclosed a loss of the stimulatory effect of Delta(9)-THC on hippocampal glutamate release in Delta(9)-THC-rats, but not in controls. In addition, l-[(3)H]-glutamate uptake was significantly lower in hippocampal slices from Delta(9)-THC-rats, where a significant decrease in glutamate transporter 1 (GLT1) and glutamate/aspartate transporter (GLAST) protein was also detected. Collectively, these data demonstrate that perinatal exposure to cannabinoids induces long-term impairment in hippocampal glutamatergic neurotransmission that persist into adolescence.
authors: Department of Neuroscience, University Politecnica delle Marche, Ancona, Italy.
authors: Pasqualina
authors: P
authors: Castaldo
authors: Simona
authors: S
authors: Magi
authors: Mauro
authors: M
authors: Cataldi
authors: Sara
authors: S
authors: Arcangeli
authors: Vincenzo
authors: V
authors: Lariccia
authors: Annamaria Assunta
authors: AA
authors: Nasti
authors: Luca
authors: L
authors: Ferraro
authors: Maria Cristina
authors: MC
authors: Tomasini
authors: Tiziana
authors: T
authors: Antonelli
authors: Tommaso
authors: T
authors: Cassano
authors: Vincenzo
authors: V
authors: Cuomo
authors: Salvatore
authors: S
authors: Amoroso
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D013759:Dronabinol / Q000633:toxicity*; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D011188:Potassium / Q000494:pharmacology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D027342:Excitatory Amino Acid Transporter 2; C493394:SLC1A3 protein, human; D018698:Glutamic Acid; D013759:Dronabinol; D011188:Potassium