Deciphering the proteomic profile of rice (Oryza sativa) bran: a pilot study.
The exact knowledge of the qualitative and quantitative protein components of rice bran is an essential aspect to be considered for a better understanding of the functional properties of this resource. Aim of the present investigation was to extract the largest number of rice bran proteins and to obtain their qualitative characterization. For this purpose, three different extraction protocols have been applied either on full-fat or on defatted rice bran. Likewise, to identify the highest number of proteins, MS data collected from 1-DE, 2-DE and gel-free procedures have been combined. These approaches allowed to unambiguously identify 43 proteins that were classified as signalling/regulation proteins (30%), proteins with enzymatic activity (30%), storage proteins (30%), transfer (5%) and structural (5%) proteins. The fact that all extraction and identification procedures have been performed in triplicate with an excellent reproducibility provides a rationale for considering the platform of proteins shown in this study as the potential proteome profile of rice bran. It also represents a source of information to evaluate better the qualities of rice bran as food resource.
Ferrari
Fabio
F
Department of Biochemistry, University of Pavia, 27100 Pavia, Italy.
Fumagalli
Marco
M
Profumo
Antonella
A
Viglio
Simona
S
Sala
Alberto
A
Dolcini
Lorenzo
L
Temporini
Caterina
C
Nicolis
Stefania
S
Merli
Daniele
D
Corana
Federica
F
Casado
Begona
B
Iadarola
Paolo
P
D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods; D004043:Dietary Fiber / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D010865:Pilot Projects; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D004043:Dietary Fiber; D010446:Peptide Fragments; D010940:Plant Proteins