Assessment of phenolic content, free-radical-scavenging capacity genotoxic and anti-genotoxic effect of aqueous extract prepared from Moricandia arvensis leaves.
The present study was undertaken to provide a set of data on the safety of an aqueous extract (AQE) from Moricandia arvensis. For this reason, Escherichia coli tested strains PQ35 and PQ37 were used to detect induction of DNA lesions by AQE. The SOS Chromotest showed that AQE induced a marginally genotoxic effect, as expressed by the induction factor (IF) value only with E. coli PQ37 tested strain (IF=1.77 at a dose of 250 microg/assay). The measurement of the anti-genotoxic activity of the AQE was also studied by inhibition of beta-galactosidase induction. A significant anti-genotoxic effect was observed with different tested doses of AQE, which suggests that M. arvensis extract has the potential to protect DNA from the action of nitrofurantoïn (NF) and free radicals generated by hydrogen peroxide (H2O2). In addition to anti-genotoxic activity, AQE showed a free-radical-scavenging capacity towards ABTS+* and DPPH*. Total phenolic content was also evaluated following Folin-Ciocalteu method and results indicated high correlation between total phenol content and anti-genotoxic and antioxidant activities for AQE, but the highest correlation was showed with its capacity to stabilize ABTS+* (R2=0.9944).
Skandrani
I
I
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Monastir, Tunisia.
Limem
I
I
Neffati
A
A
Boubaker
J
J
Ben Sghaier
M
M
Bhouri
W
W
Bouhlel
I
I
Kilani
S
S
Ghedira
K
K
Chekir-Ghedira
L
L
D016587:Antimutagenic Agents / Q000032:analysis / Q000494:pharmacology*; D052160:Benzothiazoles / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry*; D004249:DNA Damage; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology*; D005609:Free Radicals / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009153:Mutagens / Q000032:analysis / Q000494:pharmacology*; D009582:Nitrofurantoin / Q000633:toxicity; D010636:Phenols / Q000032:analysis*; D010851:Picrates / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013014:SOS Response, Genetics / Q000187:drug effects / Q000235:genetics; D013451:Sulfonic Acids / Q000494:pharmacology; D001616:beta-Galactosidase / Q000096:biosynthesis
D016587:Antimutagenic Agents; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D009153:Mutagens; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009582:Nitrofurantoin; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D001616:beta-Galactosidase