Extending the knowledge in histochemistry and cell biology.
Central to modern Histochemistry and Cell Biology stands the need for visualization of cellular and molecular processes. In the past several years, a variety of techniques has been achieved bridging traditional light microscopy, fluorescence microscopy and electron microscopy with powerful software-based post-processing and computer modeling. Researchers now have various tools available to investigate problems of interest from bird's- up to worm's-eye of view, focusing on tissues, cells, proteins or finally single molecules. Applications of new approaches in combination with well-established traditional techniques of mRNA, DNA or protein analysis have led to enlightening and prudent studies which have paved the way toward a better understanding of not only physiological but also pathological processes in the field of cell biology. This review is intended to summarize articles standing for the progress made in "histo-biochemical" techniques and their manifold applications.
Heupel
Wolfgang-Moritz
WM
Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany. heupel@uni-wuerzburg.de
Drenckhahn
Detlev
D
D000818:Animals; D003585:Cell Biology; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans