[The experimental results of GVHD following orthotropic liver transplantation].
OBJECTIVE
To analyze experimental results of Graft-versus-host disease (GVHD) after liver transplantation.


METHODS
13 cases of GVHD out of the 1013 liver transplantation between 2002-2008 were analysed. Routine blood test, liver function and microorganisms test were done in all of the 13 cases, bone marrow test was done in 5 cases, liver pathological test was done in 5 cases, cytokines were analyzed in 4 cases, chimerism test was done in 6 cases.


RESULTS
Leukocytes were reduced to various degree in all 13 cases, and were extremely low in 8 cases. Hematopoiesis was repressed in 4 cases. Normal liver function was found in 9 cases. Bacterium were found in blood, bile, wound secrete juice, excrement, phlegm of 10 cases. The pathological characteristics was in accordance with GVHD in 5 cases. The levels of IL-1 alpha, IL-1 beta, IL-2, IL-4 were low or undetectable. IL-10 was decreased in 4 cases but increased in 1 case. MCP-1, VEGF, IL-6, EGF, IL-8 were increasing or remained at high level during GVHD. TNF alpha was slightly increased. IFN gamma was only slightly changed before GVHD.


CONCLUSIONS
Chimerism is a reliable but not unique evidence of GVHD.
Yao
Gui-ling
GL
Clinical Laboratory, General Hospital of Chinese People's Armed Police, Beijing 100036, China.
Li
Wei
W
Liu
Ai-bing
AB
Liang
Hui
H
Song
Li-jie
LJ
Wang
Mei-e
ME
Liu
Yuan-ming
YM
Wang
Li
L
Wang
Hai-yan
HY
D000208:Acute Disease; D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000209:etiology; D001855:Bone Marrow Diseases / Q000097:blood / Q000209:etiology; D001856:Bone Marrow Examination; D002423:Cause of Death; D046528:Chimerism; D016207:Cytokines / Q000097:blood*; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000175:diagnosis* / Q000209:etiology / Q000401:mortality; D006801:Humans; D007378:Interleukins / Q000097:blood / Q000378:metabolism; D007970:Leukopenia / Q000097:blood / Q000209:etiology; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014182:Transplantation, Autologous; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha