title: Association of a single nucleotide polymorphism in the C-reactive protein gene (-286) with susceptibility to Plasmodium falciparum malaria.
abstract: The role of inflammation in malaria pathogenesis is not fully understood, although C-reactive protein (CRP) may have a negative influence on host immunity to infections. An upstream polymorphism, -286 (C > T > A), in the CRP gene is known to influence CRP levels. In this study, a cohort of 192 Sudanese donors, followed for malaria infection for 9 years, had their CRP -286 gene locus genotyped by pyrosequencing. The number of malaria episodes experienced by each individual over the study period was used as an index for malaria susceptibility. The prevalence of the CRP alleles A, C and T were 21%, 52% and 27%, respectively. Importantly, the A-allele, unlike the C- and T-alleles or CRP genotypes, was significantly associated with an increased number of malaria episodes, P = 0.007. The proportion of A-allele carriers among donors not known to have had malaria during the study period was 18%, whereas it was 43% and 63% among donors who had experienced 1-4 and > or =5 malaria episodes, respectively, over the same period (P = 0.002). Furthermore, the A-allele was associated with higher parasite counts. In conclusion, the CRP -286 A-allele was associated with an increased susceptibility to uncomplicated Plasmodium falciparum malaria.
authors: Department of Medical Biochemistry, Faculty of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain. gehaha2002@yahoo.com
authors: Hayder A
authors: HA
authors: Giha
authors: Amre
authors: A
authors: Nasr
authors: Mattias
authors: M
authors: Ekström
authors: Elisabeth
authors: E
authors: Israelsson
authors: Gishanthi
authors: G
authors: Arambepola
authors: David
authors: D
authors: Arnot
authors: Thor G
authors: TG
authors: Theander
authors: Marita
authors: M
authors: Troye-Blomberg
authors: Klavs
authors: K
authors: Berzins
authors: Per
authors: P
authors: Tornvall
authors: Gehad
authors: G
authors: ElGhazali
mesh_terms: D000483:Alleles; D001831:Body Temperature; D002097:C-Reactive Protein / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006451:Hemoglobin, Sickle; D006801:Humans; D008137:Longitudinal Studies; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000235:genetics* / Q000469:parasitology; D018512:Parasitemia; D010963:Plasmodium falciparum; D020641:Polymorphism, Single Nucleotide; D018709:Statistics, Nonparametric; D013397:Sudan / Q000453:epidemiology
chemical_list: D006451:Hemoglobin, Sickle; D002097:C-Reactive Protein