title: Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains.
abstract: Haemophilus parasuis is the etiologic agent of Glässer's disease in pigs, which is pathologically characterized by serofibrinous polyserositis and arthritis. H. parasuis include virulent and non-virulent strains and confirmation of virulence in H. parasuis is still dependent on experimental reproduction of the disease. Since the variability in virulence is supported by serotyping and genotyping (particularly, multilocus sequence typing [MLST]), we examined the relationship between the classification of 8 field strains by these methods and their capacity to cause disease in snatch-farrowed, colostrum-deprived piglets. The severity of clinical signs and lesions produced by the different strains correlated with the quantity of H. parasuis recovered from the lesions. However, the virulence of the strains in the animal model did not show a total correlation with their serovar or their classification by MLST. More studies are needed to identify a virulence marker that could substitute animal experimentation in H. parasuis. In addition, we reproduced disease in domestic pigs with a strain isolated from the nasal cavity of wild boars. This result indicates the existence of virulent strains of H. parasuis in wild suids, which could produce disease under appropriate circumstances, and suggests a possible source of infection for domestic pigs.
authors: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Barcelona, Spain. virginia.aragon@cresa.uab.es
authors: Virginia
authors: V
authors: Aragon
authors: Marta
authors: M
authors: Cerdà-Cuéllar
authors: Lorenzo
authors: L
authors: Fraile
authors: Mark
authors: M
authors: Mombarg
authors: Miquel
authors: M
authors: Nofrarías
authors: Alexandre
authors: A
authors: Olvera
authors: Marina
authors: M
authors: Sibila
authors: David
authors: D
authors: Solanes
authors: Joaquim
authors: J
authors: Segalés
mesh_terms: D000818:Animals; D006192:Haemophilus Infections / Q000382:microbiology* / Q000473:pathology*; D044137:Haemophilus parasuis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D012703:Serotyping; D034421:Sus scrofa / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology*; D014774:Virulence / Q000235:genetics