Impairment of stimulation ability of very-preterm neonatal monocytes in response to lipopolysaccharide.
Little is known about innate immunity of neonates, particularly for very-preterm ones, which are more susceptible to immunologic damage due to their immature immune response. This cross-sectional, descriptive study in umbilical cord blood mononuclear cells describes the differences in innate immune response between 64 healthy neonates of different gestational ages (very-preterm, preterm, full-term). CD14(+) monocytes cultured with lipopolysaccharide (LPS) or LPS + interferon-gamma showed significant lower human leukocyte antigen-DR percentages for the very-preterm group in both unstimulated and LPS-stimulated cells. No differences were found for CD40(+)-cell percentages. We observed an increase in CD80 and a decrease in CD86 within all groups when stimulated with LPS or LPS + interferon-gamma. Interleukin-12 production was lower in very-preterm neonates. Adhesion capability of neonatal monocytes was similar and independent of gestational age. In summary, very-preterm-neonatal monocytes do not completely respond to LPS and, therefore, have diminished functions compared with preterm or full-term neonates.
Pérez
Alicia
A
Laboratorio de Inmuno-Biología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Bellón
José M
JM
Gurbindo
Ma Dolores
MD
Muñoz-Fernández
Ma Angeles
MA
D000328:Adult; D017951:Antigen Presentation / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002469:Cell Separation; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D007231:Infant, Newborn / Q000276:immunology*; D007234:Infant, Premature / Q000276:immunology*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D008423:Maternal Age; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D011247:Pregnancy
D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D018664:Interleukin-12