[Dynamic changes of content of salvianolic acid in vegetative organs of Salvia miltiorrhiza].
OBJECTIVE
To determine the content of total salvianolic acid in different vegetative organs of Salvia miltiorrhiza and discover the dynamic change rules of tanshinol, protocatechuic aldehyde, caffeic acid and total salvianolic acid during the whole process of grwth.


METHODS
HPLC-ECD was used. The separation was performed on Zorbax SB-C18 (150 mm x 4.6 mm, 5.0 microm) column by gradient elution. The mobile phase consisted of CH3OH-0.4% aqueous phosphoric acid. The flow rate was 1.0 mL/min. The detection was done at 0.7 V and the column temperature was 30 degrees C.


RESULTS
The highest content of total salvianolic acid in leaf was in June and gradually dropped off till the lowest in December; The content of total salvianolic acid in leaf gradually decreased along with the growing of the leaf. The content of total salvianolic acid in root was high and consistent from July to September, but gradually dropped off till the lowest in November.


CONCLUSIONS
The leaves of Salvia miltiorrhiza can be harvested in strong growth period to achieve the comprehensive use of the herb.
Qin
Hai-Yan
HY
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. qinhaiyan03@163.com
Suo
Zhi-Rong
ZR
Liu
Wen-Zhe
WZ
D001547:Benzaldehydes / Q000378:metabolism; D002109:Caffeic Acids / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007773:Lactates / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000254:growth & development / Q000378:metabolism*; D012621:Seasons
D001547:Benzaldehydes; D002109:Caffeic Acids; D002396:Catechols; D007773:Lactates; C090844:acetylsalvianolic acid A; C005581:protocatechualdehyde