Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica.
OBJECTIVE
To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum).


METHODS
Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown.


RESULTS
A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.


CONCLUSIONS
Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
Wang
Shiping
S
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China. wsp4373383@126.com
Yu
Luxin
L
Che
Hongli
H
Chen
Xiuchun
X
Qin
Yonghua
Y
Gao
Dongmei
D
Liu
Mingshe
M
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002244:Carbon / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D003102:Colloids; D002244:Carbon