Rate determination and distribution of anammox activity in activated sludge treating swine wastewater.
This paper presents a quantitative investigation and analysis of anammox activity in sludge taken from biological swine wastewater treatment plants. An incubation experiment using a (15)N tracer technique showed anammox activity in sludge taken from 6 out of 13 plants with the rate ranging from 0.0036 micromol-N(2)/g-VSS/h to 3.1 micromol-N(2)/g-VSS/h, and in a biofilm with the highest activity at 25.8 micromol-N(2)/g-VSS/h. It is notable that 9 out of 11 sludges in which the pH was maintained between 6.6 and 8.1 retained anammox activity, while those with either a lower or higher pH did not. Moreover, anammox-positive sludge had a significantly higher concentration of NO(2)(-)-N plus NO(3)(-)-N than did anammox-negative sludge. A significant difference was not observed between anammox-positive and -negative sludge regarding BOD/NH(4)(+)-N in the influent, DO concentration in aeration tanks, and the concentrations of NH(4)(+)-N, free nitric acid, and free ammonia.
Waki
Miyoko
M
National Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan. mwaki@affrc.go.jp
Yasuda
Tomoko
T
Suzuki
Kazuyoshi
K
Sakai
Takahiro
T
Suzuki
Naoto
N
Suzuki
Ryoji
R
Matsuba
Kenji
K
Yokoyama
Hiroshi
H
Ogino
Akifumi
A
Tanaka
Yasuo
Y
Ueda
Shingo
S
Takeuchi
Mio
M
Yamagishi
Takao
T
Suwa
Yuichi
Y
D000641:Ammonia / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*; D013552:Swine; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D012722:Sewage; D000641:Ammonia