title: P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity.
abstract: Post-transcriptional gene silencing (PTGS), or RNA silencing, is one of the key mechanisms of antiviral defence used by plants. To counter this defence response, viruses produce suppressor proteins that are able to inhibit the PTGS pathway or to interfere with some of its function. The aim of this study was to evaluate the RNA silencing suppressor (RSS) activity of P0 proteins from selected European isolates of the beet-infecting poleroviruses beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV) using two different experimental systems: (i) agro-infiltration of Nicotiana benthamiana green fluorescent protein-positive plants and (ii) mechanical inoculation of Chenopodium quinoa using a beet necrotic yellow vein virus (BNYVV, genus Benyvirus) RNA3-based replicon. The results demonstrated that P0 of most BMYV isolates exhibited RSS activity, although at various efficiencies among isolates. Conversely, P0 of BChV isolates displayed no RSS activity in either of the two systems under the experimental conditions used. These results are the first reported evidence that P0 proteins of two closely related beet poleroviruses show strain-specific differences in their effects on RNA silencing.
authors: Institut de Biologie Moléculaire des Plantes, CNRS, 67084 Strasbourg, France.
authors: Anna
authors: A
authors: Kozlowska-Makulska
authors: Hubert
authors: H
authors: Guilley
authors: Marek S
authors: MS
authors: Szyndel
authors: Monique
authors: M
authors: Beuve
authors: Olivier
authors: O
authors: Lemaire
authors: Etienne
authors: E
authors: Herrbach
authors: Salah
authors: S
authors: Bouzoubaa
mesh_terms: D000595:Amino Acid Sequence; D027461:Beta vulgaris / Q000821:virology*; D049452:Green Fluorescent Proteins; D052679:Luteoviridae / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D014764:Viral Proteins; D049452:Green Fluorescent Proteins