title: Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease.
abstract: OBJECTIVE
  Multiple myeloma (MM) is characterized by a high incidence of osteolytic bone lesions, which have been previously correlated with the gene expression profiles of MM cells. The aim of this study was to investigate the transcriptional patterns of cells in the bone microenvironment and their relationships with the presence of osteolysis in MM patients.
  
  
  METHODS
  Both mesenchymal (MSC) and osteoblastic (OB) cells were isolated directly from bone biopsies of MM patients and controls to perform gene expression profiling by microarrays and real-time polymerase chain reaction on selected bone-related genes.
  
  
  RESULTS
  We identified a series of upregulated and downregulated genes that were differentially expressed in the MSC cells of osteolytic and nonosteolytic patients. Comparison of the osteolytic and nonosteolytic samples also showed that the MSC cells and OB had distinct transcriptional patterns. No significantly modulated genes were found in the OBs of the osteolytic and nonosteolytic patients.
  
  
  CONCLUSIONS
  Our data suggest that the gene expression profiles of cells of the bone microenvironment are different in MM patients and controls, and that MSC cells, but not OBs, have a distinct transcriptional pattern associated with the occurrence of bone lesions in MM patients. These data support the idea that alterations in MSC cells may be involved in MM bone disease.
authors: Dipartimento di Scienze Mediche, Università di Milano e U.O. Ematologia 1, Fondazione IRCCS Policlinico, Milan, Italy.
authors: Katia
authors: K
authors: Todoerti
authors: Gina
authors: G
authors: Lisignoli
authors: Paola
authors: P
authors: Storti
authors: Luca
authors: L
authors: Agnelli
authors: Francesca
authors: F
authors: Novara
authors: Cristina
authors: C
authors: Manferdini
authors: Katia
authors: K
authors: Codeluppi
authors: Simona
authors: S
authors: Colla
authors: Monica
authors: M
authors: Crugnola
authors: Manuela
authors: M
authors: Abeltino
authors: Marina
authors: M
authors: Bolzoni
authors: Valentina
authors: V
authors: Sgobba
authors: Andrea
authors: A
authors: Facchini
authors: Giorgio
authors: G
authors: Lambertenghi-Deliliers
authors: Orsetta
authors: O
authors: Zuffardi
authors: Vittorio
authors: V
authors: Rizzoli
authors: Antonino
authors: A
authors: Neri
authors: Nicola
authors: N
authors: Giuliani
mesh_terms: D016723:Bone Remodeling / Q000235:genetics; D001842:Bone and Bones / Q000473:pathology*; D002455:Cell Division; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D009101:Multiple Myeloma / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D010014:Osteolysis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger