title: [Effect of metabolism activation on the oxidative cell damage induced by cigarette smoking].
abstract: OBJECTIVE
  To explore the effect of metabolic activation on the oxidative cell damage induced by mainstream smoke (MS).
  
  
  METHODS
  A549 cells were treated with different concentrations of MS for 2 hours in the presence and absence of S9 mix. ROS levels were determined. DNA and chromosome damages were detected. The activities of SOD and GSH-Px were measured.
  
  
  RESULTS
  With different concentrations of MS treatment, the contents of ROS in the cells with S9 mix were significantly higher than those without S9 mix (P<0.05). More serious chromosome and DNA damages in the cells with S9 mix were observed than those without S9 mix. The micronucleus rate, comet rate, L Tail and OTM in the cells with S9 mix were significantly higher than those without S9 mix (P<0.05). With increased dose of MS, the activities of SOD and GSH-Px decreased in both cells with and without S9 mix. But the tendency of decrease in the cells with S9 mix was more obvious than those without S9 mix (P<0.05).
  
  
  CONCLUSIONS
  MS induces oxidative damage in A549 cells and the metabolic activations could increase the oxidative stress and potentiate the cellular genotoxicity.
authors: Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Ling
authors: L
authors: Wang
authors: Qing-Ying
authors: QY
authors: Luo
authors: Li
authors: L
authors: Zhang
authors: Mei
authors: M
authors: Wu
authors: Zun-Zhen
authors: ZZ
authors: Zhang
mesh_terms: D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects; D015162:Micronucleus Tests; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D012906:Smoke