title: Pyrogallol-induced endothelial cell death is related to GSH depletion rather than ROS level changes.
abstract: Pyrogallol (PG) as a polyphenol compound induces apoptosis in several types of cells. Here, we evaluated the effects of PG on endothelial cells (ECs), especially calf pulmonary artery endothelial cells (CPAEC) in relation to the cell growth, ROS and glutathione (GSH) levels. PG dose-dependently inhibited the growth of CPAEC and human umbilical vein endothelial cells (HUVEC) at 24 h. PG also induced apoptosis in CPAEC, which was accompanied by the loss of mitochondrial membrane potential (MMP; DeltaPsim). PG decreased ROS level including O2*- and PG dose-dependently increased GSH depleted cell number in both EC types. N-acetyl-cysteine (NAC; a well-known antioxidant) increased ROS levels in PG-treated CPAEC with the prevention of cell death and GSH depletion. In conclusion, PG inhibited the growth of ECs, especially CPAEC via apoptosis. PG-induced EC death was related to GSH depletion rather than ROS level changes.
authors: Department of Physiology, Medical School, Centers for Healthcare Technology Development Institute for Medical Sciences Chonbuk National University, Jeonju, Republic of Korea.
authors: Yong Hwan
authors: YH
authors: Han
authors: Hwa Jin
authors: HJ
authors: Moon
authors: Bo Ra
authors: BR
authors: You
authors: Sung Zoo
authors: SZ
authors: Kim
authors: Suhn Hee
authors: SH
authors: Kim
authors: Woo Hyun
authors: WH
authors: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D002417:Cattle; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000187:drug effects*; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D011748:Pyrogallol / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D011748:Pyrogallol; D005978:Glutathione; D010100:Oxygen