Influence of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin expression in pancreatic tissue of rats with severe acute pancreatitis.
OBJECTIVE
Baicalin reduces the severity of severe acute pancreatitis (SAP) in a rat model. This study was carried out to examine the effect of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin protein expression in the pancreas of rats with SAP.


METHODS
Rats with SAP were randomly assigned to untreated, baicalin-treated, octreotide-treated and sham-operated group. The TNF-alpha mRNA expression level, caspase-3 and P-selectin protein expression levels of pancreatic tissue were measured 3, 6 and 12 hours after operation.


RESULTS
The caspase-3 protein expression level was significantly higher in the treated groups than that in the untreated group at 3 hours (p <0.01). P-selectin protein expression level in pancreas of the treated groups was significantly lower than that of the untreated group (p <0.05 or <0.01). TNF-alpha mRNA expression level in pancreas decreased in baicalintreated group while untreated group increased at different time points (p <0.05).


CONCLUSIONS
Baicalin increases caspase-3 expression and inhibits TNF-alpha mRNA and P-selectin protein expression in pancreatic tissue of SAP rats.
Xiping
Zhang
Z
Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China. zxp99688@vip.163.com
Hua
Tian
T
Jie
Zhang
Z
Li
Chen
C
Yang
Cai
C
Bei
Lu
L
Qijun
Yang
Y
Jing
Ye
Y
Wengqin
Yuan
Y
Ninnin
Zhou
Z
D000818:Animals; D053148:Caspase 3 / Q000187:drug effects*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008297:Male; D019007:P-Selectin / Q000187:drug effects*; D010195:Pancreatitis / Q000188:drug therapy / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*
D004791:Enzyme Inhibitors; D005419:Flavonoids; D019007:P-Selectin; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin; D053148:Caspase 3