title: Estimating the integrity of aged DNA samples by CE.
abstract: A CE/UV method was developed to separate by a micellar system the four DNA bases and other five purinic-pyrimidinic compounds (5-methyl-cytosine, uracil, xanthyne, hypoxanthyne and 5-bromo-uracil). Selectivity, precision, accuracy and sensitivity were assessed and proved to be suitable for the analysis of the primary structure of DNA. This method was adopted to study 16 aged samples including two Egyptian mummies, formaldehyde-fixed paraffin-embedded tissues and other forensic specimens. Lower relative values of the four canonical unmodified DNA bases (uDNAb) and more complex pherograms were found in the aged samples when compared with the modern controls. The results of the CE analysis, together with those obtained by classical molecular methods (agarose gel electrophoresis, DNase I and RNase A assays, and UV spectrophotometry), were finally evaluated for assessing the reliability of STR typing. Since samples with low uDNAb showed no amplification or unreliable STR profiles, the uDNAb value is discussed as a further quality criterion in the evaluation of the genetic data obtained from aged samples.
authors: Department of Sciences of Public Health, University of Trieste, Italy. fattorin@univ.trieste.it
authors: Paolo
authors: P
authors: Fattorini
authors: Giorgio
authors: G
authors: Marrubini
authors: Ugo
authors: U
authors: Ricci
authors: Fabio
authors: F
authors: Gerin
authors: Pierangela
authors: P
authors: Grignani
authors: Solange Sorçaburu
authors: SS
authors: Cigliero
authors: Andrea
authors: A
authors: Xamin
authors: Elisabetta
authors: E
authors: Edalucci
authors: Giancarlo
authors: G
authors: La Marca
authors: Carlo
authors: C
authors: Previderé
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry*; D053836:DNA Degradation, Necrotic; D019075:Electrophoresis, Capillary / Q000379:methods*; D005404:Fixatives; D018732:Forensic Anthropology / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D005557:Formaldehyde; D006801:Humans; D009106:Mummies; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D020080:Tandem Repeat Sequences
chemical_list: D005404:Fixatives; D005557:Formaldehyde; D004247:DNA