title: Magnetically enhanced high-specificity virus detection using bio-activated magnetic nanoparticles with antibodies as labeling markers.
abstract: This study describes magnetically driven suppression of cross-reactions among molecules. First, the magnetic nanoparticles are coated with bio-probes and dispersed in liquid. The bio-probes can then bind with homologous or heterologous bio-targets. When alternating-current (ac) magnetic fields are applied, magnetic nanoparticles rotate driven by ac magnetic fields. Thus, the bio-targets bound on the surface of magnetic nanoparticles experience a centrifugal force. The centrifugal force can be manipulated by adjusting the angular frequency of the rotating magnetic nanoparticles. The angular frequency is determined by the applied ac magnetic field frequency. Since the binding force for good binding is much higher than that of poor binding, frequency manipulation is needed for the centrifugal force to be higher than the poor-binding force but lower than the good-binding force. Therefore, poor binding which contributes to cross reactions between molecules can be suppressed efficiently by control of the ac magnetic field frequency.
authors: Institute of Electro-optical Science and Technology, National Taiwan Normal University, 88 Sec 4, Ting-chou Road, Taipei 116, Taiwan.
authors: S Y
authors: SY
authors: Yang
authors: W C
authors: WC
authors: Wang
authors: C B
authors: CB
authors: Lan
authors: C H
authors: CH
authors: Chen
authors: J J
authors: JJ
authors: Chieh
authors: H E
authors: HE
authors: Horng
authors: Chin-Yih
authors: CY
authors: Hong
authors: H C
authors: HC
authors: Yang
authors: C P
authors: CP
authors: Tsai
authors: C Y
authors: CY
authors: Yang
authors: I C
authors: IC
authors: Cheng
authors: W C
authors: WC
authors: Chung
mesh_terms: D000906:Antibodies; D000956:Antigens, Viral / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D008280:Magnetics; D053758:Nanoparticles; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D000956:Antigens, Viral