Mucilage and polysaccharides in the halophyte plant species Kosteletzkya virginica: localization and composition in relation to salt stress.
Mucilage is thought to play a role in salinity tolerance in certain halophytic species by regulating water ascent and ion transport. The localization and composition of mucilage in the halophyte Kosteletzkya virginica was therefore investigated. Plants were grown in a hydroponic system in the presence or absence of 100mM NaCl and regularly harvested for growth parameter assessment and mucilage analysis with the gas liquid chromatography method. NaCl treatment stimulated shoot growth and biomass accumulation, had little effect on shoot and root water content, and reduced leaf water potential (Psi(w)), osmotic potential (Psi(s)) as well as stomatal conductance (g(s)). Mucilage increased in shoot, stems and roots in response to salt stress. Furthermore, changes were also observed in neutral monosaccharide components. Levels of rhamnose and uronic acid increased with salinity. Staining with a 0.5% alcian blue solution revealed the presence of mucopolyssacharides in xylem vessels and salt-induced mucilaginous precipitates on the leaf abaxial surface. Determination of ion concentrations showed that a significant increase of Na(+) and a decrease of K(+) and Ca(2+) simultaneously occurred in tissues and in mucilage under salt stress. Considering the high proportion of rhamnose and uronic acid in stem mucilage, we suggest that the pectic polysaccharide could be involved in Na(+) fixation, though only a minor fraction of accumulated sodium appeared to be firmly bound to mucilage.
Edmond Ghanem
Michel
M
Groupe de Recherche en Physiologie Végétale, Université catholique de Louvain (UCL), Croix du Sud 5 Bte 13, 1348 Louvain-la-Neuve, Belgium. Michel.Ghanem@uclouvain.be
Han
Rui-Ming
RM
Classen
Birgit
B
Quetin-Leclerq
Joëlle
J
Mahy
Gregory
G
Ruan
Cheng-Jiang
CJ
Qin
Pei
P
Pérez-Alfocea
Francisco
F
Lutts
Stanley
S
D000269:Adhesives / Q000032:analysis* / Q000378:metabolism; D019660:Malvaceae / Q000737:chemistry / Q000502:physiology*; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry / Q000502:physiology; D018520:Plant Shoots / Q000737:chemistry / Q000502:physiology; D018547:Plant Stems / Q000737:chemistry / Q000502:physiology; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D012210:Rhamnose / Q000032:analysis / Q000378:metabolism; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000737:chemistry / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology; D014574:Uronic Acids / Q000032:analysis / Q000378:metabolism; D052584:Xylem / Q000502:physiology
D000269:Adhesives; D011134:Polysaccharides; D014574:Uronic Acids; D012210:Rhamnose