title: Cloning, sequencing and expression analysis of the equine hepcidin gene by real-time PCR.
abstract: Equine serum or plasma iron concentration drops quickly during inflammation. Accumulation of iron inside macrophages and reduction of the intestinal absorption of this element cause hypoferremia during systemic inflammatory processes. These mechanisms are mediated by hepcidin, a 25 amino acids peptide synthesized mainly in the liver in response to iron stores and inflammation. Hepcidin is an important peptide for systemic iron homeostasis and also has antibacterial and antifungal activities. Hepcidin up-regulation is particularly useful during acute inflammation, especially before adaptive immunity occurs, restricting iron availability necessary for pathogenic microorganism growth. Hepcidin gene products have been previously characterized in man, non-human primates, rat, mouse, dog swine, cattle, fishes, reptiles and birds; but until now not in the horse. We have cloned and sequenced equine hepcidin mRNA and performed hepcidin expression analysis in different tissues collected from four healthy horses. The deduced precursor of equine hepcidin was most homologous to Bos taurus and Sus scrofa. The expressed profile of equine hepcidin in liver was very high. Expression in cervical spinal cord and cerebral cortex was much lower than liver but higher than lung, duodenum, stomach, spleen, kidney, skeletal muscle and bladder. This sequence will be helpful for additional studies on iron metabolism and inflammatory process in horses.
authors: Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Sao Paulo State University, Unesp, FMVZ, Botucatu, SP 18618000, Brazil.
authors: José P
authors: JP
authors: Oliveira Filho
authors: Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Sao Paulo State University, Unesp, FMVZ, Botucatu, SP 18618000, Brazil.
authors: Peres R
authors: PR
authors: Badial
authors: Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Sao Paulo State University, Unesp, FMVZ, Botucatu, SP 18618000, Brazil.
authors: Paulo H J
authors: PHJ
authors: Cunha
authors: Department of Immunology and Microbiology, Sao Paulo State University, Unesp, IBB, Botucatu, SP 18618000, Brazil.
authors: Taís F
authors: TF
authors: Cruz
authors: Department of Immunology and Microbiology, Sao Paulo State University, Unesp, IBB, Botucatu, SP 18618000, Brazil.
authors: João P
authors: JP
authors: Araújo
authors: Department of Clinical Science, Cornell University, Ithaca, NY 14853, USA.
authors: Thomas J
authors: TJ
authors: Divers
authors: Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA.
authors: Nena J
authors: NJ
authors: Winand
authors: Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Sao Paulo State University, Unesp, FMVZ, Botucatu, SP 18618000, Brazil. Electronic address: asborges@fmvz.unesp.br.
authors: Alexandre S
authors: AS
authors: Borges
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D064451:Hepcidins; D006736:Horses / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; C578702:HAMP protein, human; C579599:Hamp protein, mouse; C578703:Hamp protein, rat; D064451:Hepcidins