title: Fluorimetric lead detection in a microfluidic device.
abstract: A microfabricated device has been developed for the selective detection of lead in water. It is based on the use of a selective and sensitive fluorescent molecular sensor for lead (Calix-DANS4) which contains a calix[4]arene bearing four dansyl groups. The microchip-based lead sensor contains a Y-shape microchannel equipped with a passive mixer and moulded on a glass substrate. An optimization of the microcircuit length has been performed in order to have a full complexation of the Calix-DANS4. The detection is performed by using a configuration in which the sensing molecules are excited by two optical fibres, each one connected to a 365 nm UV LED, and the light collection is made by another optical fibre with a photomultiplier. By using this configuration we have shown the possibility to detect lead with a detection limit of 5 ppb. The effect of interfering cations such as calcium has been evaluated. The obtained measurements have been validated by an alternative method (ASV).
authors: PPSM, ENS Cachan, CNRS, Wilson, 94230, Cachan, France.
authors: Liyun
authors: L
authors: Zhao
authors: Ting
authors: T
authors: Wu
authors: Jean-Pierre
authors: JP
authors: Lefèvre
authors: Isabelle
authors: I
authors: Leray
authors: Jacques A
authors: JA
authors: Delaire
mesh_terms: D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D007854:Lead / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D007854:Lead