[Expression of bone morphogenetic protein-2 in fetal membranes from pregnant women with premature rupture of membranes and its clinic significance].
OBJECTIVE
To study the relationship between bone morphogenetic protein-2 (BMP-2) and premature rupture of membranes (PROM).


METHODS
Ninety gravidas giving birth at 4 hospitals in Chongqing from January 2006 to January 2007 were selected and divided into 5 groups (18 in each): term labor group ( with intact membranes), term PROM group, preterm labor group (with intact membranes), preterm PROM (PPROM) group and control group (term pregnancy not in labor). The expression of BMP-2 in the chorioamniotic membranes of each group were detected by streptavidin-peroxidase methods and its relationship with PROM plus histological chorioamnionitis were analyzed.


RESULTS
(1) BMP-2 was detected in the cytoplasm of epithelial and interstitial cells of membranes in each group. (2) BMP-2 expression in term labor group, term PROM group, preterm labor group and PPROM group were 0.0137 +/- 0.0094, 0.0269 +/- 0.0127, 0.0273 +/- 0.0145, 0.0456 +/- 0.0114, respectively, which higher than that of the control group 0.0060 +/- 0.0079 (P < 0.01). BMP-2 expression in the term PROM group was higher than that of the term labor group (P < 0.01), that in the PPROM was higher than in the preterm labor group and term PROM group (P < 0.01). (3) Pathological examination found that 17 out of the 90 women with histologic chorioamnionitis, of which 13 (36%) came from the term PROM and PPROM group with a higher level of BMP-2 expression than those without chorioamnionitis (0.0201 +/- 0.0107 vs 0.0105 +/- 0.0092, P < 0.05).


CONCLUSIONS
The expression of BMP-2 in chorioamniotic membranes is closely related to PROM.
Li
Qing-Shu
QS
Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Qi
Hong-Bo
HB
D000293:Adolescent; D000328:Adult; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002821:Chorioamnionitis / Q000209:etiology / Q000378:metabolism; D002823:Chorion / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism*; D005865:Gestational Age; D006801:Humans; D007743:Labor, Obstetric / Q000378:metabolism; D007752:Obstetric Labor, Premature / Q000378:metabolism; D011247:Pregnancy; D055815:Young Adult
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003094:Collagen