title: Ocular dirofilariosis by Dirofilaria immitis in a dog: first case report from Europe.
abstract: A five-year-old, entire female mixed-breed dog was presented with corneal oedema and episcleral hyperaemia in the left eye. The ophthalmological examination revealed the presence of a free-swimming nematode in the anterior chamber. Circulating microfilariae were not observed by a modified Knott test nor were adult antigens detected in serum by a commercial ELISA. The parasite was surgically removed from the dog's eye, but its anterior end was damaged during the surgery. Based on the morphology of the posterior end, the nematode was preliminarily identified as a male Dirofilaria immitis. The species identification was confirmed by PCR amplification and sequencing of the mitochondrial coxI and 12S rDNA genes, using a DNA barcoding approach. Although other cases of ocular dirofilariosis by D. immitis have been previously recorded in Australia and the United States, the case reported herein is the first in a dog from Europe.
authors: Prof D. Otranto Dipartimento di Sanitá Pubblica e Zootecnia, Universitá degli Studi di Bari, 70010 Bari, Italy.
authors: F
authors: F
authors: Dantas-Torres
authors: R P
authors: RP
authors: Lia
authors: M
authors: M
authors: Barbuto
authors: M
authors: M
authors: Casiraghi
authors: A
authors: A
authors: Crovace
authors: L
authors: L
authors: Caligiani
authors: C
authors: C
authors: Genchi
authors: D
authors: D
authors: Otranto
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis; D004183:Dirofilaria immitis / Q000302:isolation & purification; D004184:Dirofilariasis / Q000175:diagnosis* / Q000601:surgery; D004283:Dog Diseases / Q000175:diagnosis* / Q000601:surgery; D004285:Dogs; D005060:Europe / Q000453:epidemiology; D015822:Eye Infections, Parasitic / Q000175:diagnosis / Q000601:surgery / Q000662:veterinary*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018509:DNA, Helminth