[Sequence analysis and identification of two novel HLA alleles HLA-B*9536 and HLA-B*4612].
OBJECTIVE
To identify two novel HLA alleles HLA-B*9536 and B*4612, in an individual.


METHODS
DNA was extracted from whole blood by Invitrogen DNA extraction kit. The amplification for HLA-B exons 2-4 of the proband was performed separately with allele group specific primers and the PCR products were directly sequenced for exons 2-4 in both direction.


RESULTS
There were two novel HLA-B alleles in the proband. The sequences of the two alleles have been submitted to GenBank (EU081878 and EU081879). The two alleles have been officially named as B*9536 and B*4612 by the WHO Nomenclature Committee. The sequence of exons 2-4 of HLA-B*9536 showed one nucleotide difference in exon 3 at position 544 (G to A) comparing with the closest allele B*1505, which resulted in an amino acid change from Ala to Thr at codon 158. In the HLA-B*4612 allele, there was one nucleotide change in exon 3 at position 363 (G to A), when compared to the closest allele B*4601, which lead to an amino acid change from Arg to Ser at codon 97.


CONCLUSIONS
Two novel HLA-B alleles were identified in one individual and have been officially named by the WHO Nomenclature Committee.
He
Yanmin
Y
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety of Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
Zhang
Wei
W
He
Junjun
J
Wang
Wei
W
Han
Zhedong
Z
Chen
Nanying
N
Zhu
Faming
F
Yan
Lixing
L
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
D015235:HLA-B Antigens; C530295:HLA-B*15:136 antigen; C530296:HLA-B*46:12 antigen; D059910:HLA-B15 Antigen