A device to measure oxygen consumption during the hypothermic perfusion of the liver.
This work deals with the construction and performance of a device designed to measure the oxygen consumption by the liver during hypothermic perfusion in the rat model. Due to its simple design and the utilization of standard materials, it could serve to determine the role of oxygenation during hypothermic perfusion of the liver. The system consists of a reservoir containing the preservation solution, a peristaltic pump and an internal oxygenator made of silicone tube. A five ports manifold connects the circulation to the liver (inflow), to a hydrostatic manometer and to two sample ports; the liver outflow and temperature sensor or gas calibration. Finally the exit port connects the circulation fluid with an oxygen electrode. The preservation solution is pumped through the liver at a constant pressure (77 i 15 mm H2O) and a perfusion flow of 0.39 - 0.49 mL per min per g liver. To test the system, two to four hours perfusion experiments were performed, at temperatures of 5 and 10 degree C. Two preservation solutions were evaluated: Custodiol and Bes-Gluconate-Sucrose. The solubility of oxygen in the preservation solutions was determined, and the oxygen consumption by preserved rat livers was measured.
Rodriguez
Joaquín V
JV
Farmacología, Departamento Ciencias, Fisiologicas, Facultad de Ciencias, Bioquimicas y Farmaceuticas, Rosario, Argentina. jrodrig@fbioyf.unr.edu.ar
Federico
María Belen
MB
Pizarro
María Dolores
MD
Guibert
Edgardo E
EE
Quintana
Alejandra B
AB
Scandizzi
Angel L
AL
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D004566:Electrodes; D004864:Equipment and Supplies; D005942:Gluconates / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007035:Hypothermia / Q000473:pathology / Q000503:physiopathology*; D007260:Infusion Pumps; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008365:Manometry; D010101:Oxygen Consumption / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
C039984:Bretschneider cardioplegic solution; D003451:Cryoprotective Agents; D005942:Gluconates; D008353:Mannitol; D011343:Procaine; D013395:Sucrose; D011189:Potassium Chloride; D005947:Glucose; C030691:gluconic acid