Yeast acetic acid-induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1.
To investigate the role of cytochrome c (cyt c) release in yeast acetic acid-induced programmed cell death (AA-PCD), wild type (wt) and cells lacking metacaspase (Deltayca1), cytochrome c (Deltacyc1,7) and both (Deltacyc1,7Deltayca1) were compared for AA-PCD occurrence, hydrogen peroxide (H(2)O(2)) production and caspase activity. AA-PCD occurs in Deltacyc1,7 and Deltacyc1,7Deltayca1 cells slower than in wt, but similar to that in Deltayca1 cells, in which no cytochrome c release occurs. Both H(2)O(2) production and caspase activation occur in these cells with early and extra-activation in Deltacyc1,7 cells. We conclude that alternative death pathways can be activated in yeast AA-PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it.
Guaragnella
Nicoletta
N
CNR, Istituto di Biomembrane e Bioenergetica, Bari, Italy.
Bobba
Antonella
A
Passarella
Salvatore
S
Marra
Ersilia
E
Giannattasio
Sergio
S
D019342:Acetic Acid / Q000494:pharmacology; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid