Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed gamma-glutamylmethylamide synthetase and dried yeast cells.
Gamma-glutamylmethylamide synthetase and dried baker's yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mM theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD(+).
Yamamoto
Sachiko
S
Department of Chemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan. s_yamamoto@ks.kyorin-u.ac.jp
Morihara
Yosuke
Y
Wakayama
Mamoru
M
Tachiki
Takashi
T
D019731:Carbon-Nitrogen Ligases / Q000737:chemistry* / Q000378:metabolism*; D003956:Dialysis; D004735:Energy Transfer; D005285:Fermentation; D005971:Glutamates / Q000096:biosynthesis*; D008567:Membranes, Artificial; D012441:Saccharomyces cerevisiae / Q000166:cytology*
D005971:Glutamates; D008567:Membranes, Artificial; C026166:theanine; D019731:Carbon-Nitrogen Ligases; C020661:gamma-glutamylmethylamide synthetase