Thermaerobacter composti sp. nov., a novel extremely thermophilic bacterium isolated from compost.
A novel extremely thermophilic bacterium (Ni80T) was isolated from food sludge compost in Japan. Cells were Gram-positive, spore-forming, strictly-aerobic and rod-shaped with peritrichous flagella. The novel isolate grew at pH 6.5-10.5 but not at pH 11.0 (optimum pH 8.0) and 52-79 degrees C but not 80 degrees C (optimum 70 degrees C). The strain could not grow in more than 1% NaCl. The G+C content of the genomic DNA is 72 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Ni80T belonged to the genus Thermaerobacter, and that the strain was most closely related to Thermaerobacter subterraneus ATCC BAA-137T (98.2%), Thermaerobacter nagasakiensis JCM 11223T (98.2%), Thermaerobacter marianensis JCM 10246T (98.0%) and Thermaerobacter litoralis JCM 13210T (97.7%). DNA-DNA hybridization showed relatedness values of less than 10% with these species. On the basis of phenotypic and molecular data, strain Ni80(T) represent a novel species of the genus Thermaerobacter, for which the name Thermaerobacter composti sp. nov. is proposed. The type strain is Ni80T (=JCM 15650T =ATCC BAA-1731T).
Yabe
Shuhei
S
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd., Miyagi 989-1311, Japan. kennan-6@kennan-e.co.jp
Kato
Atsushi
A
Hazaka
Masaru
M
Yokota
Akira
A
D001420:Bacteria, Aerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis*; D006358:Hot Temperature; D007564:Japan; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000302:isolation & purification; D012987:Soil
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil