Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques.
Vitamin B(12) was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B(12)/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B(12) for analysis. The presence of the K(+) adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization-mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.
Madhu
Arenahalli Ningegowda
AN
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 020, India.
Giribhattanavar
Prashant
P
Narayan
Mandayam Singara
MS
Prapulla
Siddalingaiya Gurudutt
SG
D000332:Aerobiosis; D000693:Anaerobiosis; D002712:Chlorides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D019936:Probiotics / Q000378:metabolism*; D013997:Time Factors; D014805:Vitamin B 12 / Q000096:biosynthesis*; D017967:Zinc Compounds / Q000494:pharmacology
D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D014805:Vitamin B 12