Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy.
Total internal reflection (TIR) holographic microscopy uses a prism in TIR as a near-field imager to perform quantitative phase microscopy of cell-substrate interfaces. The presence of microscopic organisms, cell-substrate interfaces, adhesions, and tissue structures on the prism's TIR face causes relative index of refraction and frustrated TIR to modulate the object beam's evanescent wave phase front. We present quantitative phase images of test specimens such as Amoeba proteus and cells such as SKOV-3 and 3T3 fibroblasts.
Ash
William M
WM
Digital Holography and Microscopy Laboratory, Department of Physics, University of South Florida, Tampa, Florida 33620, USA. wash@mail.usf.edu
Krzewina
Leo
L
Kim
Myung K
MK
D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured / Q000166:cytology* / Q000502:physiology*; D006696:Holography / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D010783:Photometry / Q000379:methods*; D012031:Refractometry / Q000379:methods*