Chronic inflammatory demyelinating polyneuropathy sera inhibit axonal growth of mouse dorsal root ganglion neurons by activation of Rho-kinase.
Clinical course and prognosis are variable among patients with chronic inflammatory demyelinating polyneuropathy (CIDP), whereas the extent of axonal degeneration is the major prognostic factor. We studied the effects of sera from CIDP patients on axonal growth in cultured mouse dorsal root ganglion neurons. Compared with control sera, CIDP sera prominently suppressed axonal outgrowth of dorsal root ganglion neurons and shortened axonal length. The inhibitory activity was abolished by adding Y27632, a Rho-kinase inhibitor. These findings suggest that CIDP sera inhibit axonal elongation by Rho-kinase activation, and some serum factors may be responsible for development of axonal degeneration in CIDP.
Taniguchi
Junko
J
Department of Neurology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
Sawai
Setsu
S
Mori
Masahiro
M
Kubo
Takekazu
T
Kanai
Kazuaki
K
Misawa
Sonoko
S
Isose
Sagiri
S
Yamashita
Toshihide
T
Kuwabara
Satoshi
S
D000328:Adult; D000577:Amides / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000097:blood* / Q000473:pathology*; D011725:Pyridines / Q000494:pharmacology; D044967:Serum; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology
D000577:Amides; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases