[Immunogenicity of Staphylococcus aureus recombinant clumping factor A].
In order to characterize the immunogenicity and immunoprotection of the Staphylococcus aureus (S. aureus) surface protein Clumping factor A (ClfA), we amplified clfa genes from S. aureus Newman strain, Wood46 strain and HLJ23-1. The clfa gene from Newman strain was subsequently inserted into pQE-30 vector and the recombinant plasmid was transformed into Escherichia coli strain M15 (pREP4). The recombinant ClfA protein was expressed and purified. Then, we immunized mice with the purified recombinant protein. The antibody level and the concentration of cytokines were measured by enzyme-linked immunosorbent assay. Finally, immunized mice were challenged with S. aureus Newman, Wood46 and HLJ23-1. These results suggested that clfa gene sequences were highly conserved, and the recombinant ClfA was expressed correctly with good antigenicity. The antibody titer and the concentration of cytokines in the immunized groups increased significantly (P < 0.05) compared with control, and the mice in the immunized groups were protected against the challenge strains to some extent. These results showed that the ClfA had high immunogenicity and immunoprotective potential.
Feng
Hao
H
School of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Liu
Lefeng
L
Chi
Jiaqi
J
Wang
Ning
N
Li
Runting
R
Tong
Chunyu
C
Ma
Jinzhu
J
Zhu
Zhanbo
Z
Cui
Yudong
Y
D000818:Animals; D003030:Coagulase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007114:Immunization; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000472:pathogenicity
C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D011994:Recombinant Proteins