Protective effect of glutathione S-transferase-fused mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis.
Recent studies have demonstrated that immunization with nontoxic mutant staphylococcal enterotoxin C (mSEC) provides protection against Staphylococcus aureus infection in mouse models. In the present study, we investigated whether vaccination with a glutathione S-transferase-fused SEC (GST-mSEC) can protect against S. aureus-induced bovine mastitis. Cows were immunized with the GST-mSEC plus alum adjuvant and then challenged with viable S. aureus by an intramammary route. The results showed that immunization with GST-mSEC-induced production of SEC-specific antibodies in sera and the high titers of antibodies could persist for over 12 weeks. Importantly, immunization with GST-mSEC also induced production of SEC-specific antibodies in milk. The somatic cell counts in the milk from S. aureus challenged quarters of vaccinated lactating cows were significantly lower than those of the non-vaccinated control animals. Furthermore, the sera from GST-mSEC-immunized cows significantly inhibited interferon-gamma and tumor necrosis factor-alpha production from mouse spleen cells induced by wild-type SEC. These results suggest that vaccination with GST-mSEC provides protection against S. aureus-induced bovine mastitis and that the protection might be mediated by SEC-neutralizing antibodies.
Cui
Jing-Chun
JC
Department of Preventive Veterinary Science, School of Veterinary Medicine, Northeastern Agricultural University, Harbin 150030, PR China; Department of Bio-Engineering, College of Life Science, Dalian Nationalities University, Dalian 116620, PR China.
Zhang
Bao-Jun
BJ
Department of Prevention Veterinary Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
Lin
Yan-Chun
YC
Department of Prevention Veterinary Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
Wang
Quan-Kai
QK
Department of Prevention Veterinary Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
Qian
Ai-Dong
AD
Department of Prevention Veterinary Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
Nakane
Akio
A
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.
Hu
Dong-Liang
DL
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan. Electronic address: hudl@cc.hirosaki-u.ac.jp.
Tong
Guang-Zhi
GZ
Department of Preventive Veterinary Science, School of Veterinary Medicine, Northeastern Agricultural University, Harbin 150030, PR China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: gztong@shvri.ac.cn.
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D002417:Cattle; D016207:Cytokines / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology* / Q000627:therapeutic use; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology* / Q000627:therapeutic use; D008414:Mastitis, Bovine / Q000276:immunology / Q000517:prevention & control*; D008892:Milk / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000627:therapeutic use; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use*
D057134:Antibodies, Neutralizing; D016207:Cytokines; D004768:Enterotoxins; D011993:Recombinant Fusion Proteins; D018089:Superantigens; D014614:Vaccines, Synthetic; C034959:enterotoxin C, staphylococcal; D005982:Glutathione Transferase