Significant changes in the transesterification activity of free and mesoporous-immobilized Rhizopus oryzae lipase in ionic liquids.
We examined the activity of free Rhizopus oryzae lipase (ROL) and ROL immobilized on mesoporous materials in transesterification reactions in various dialkylimidazolium-cation based ionic liquids. For free ROL, the highest activity (0.39 U/mg protein) was obtained in [OMIm][PF(6)] followed by that (0.28 U/mg protein) in [BMIm][PF(6)]. Specific activities of ROL immobilized on mesocellular foam (MCF) were only 0.47 and 0.43 U/mg protein in [OMIm][PF(6)] and [BMIm][PF(6)], respectively. However, the specific activities of ROL immobilized on octadecyl functionalized MCF (C(18)-MCF) increased significantly to 15.64 and 14.84 U/mg protein in [OMIm][PF(6)] and [BMIm][PF(6)], respectively. Consequently, ROL immobilized on C(18)-MCF is a promising biocatalyst for biotransformation reactions in ionic liquids.
Shakeri
Mozaffar
M
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. mozaffar@organ.su.se
Kawakami
Koei
K
D002087:Butyrates / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism; D004951:Esterification; D052578:Ionic Liquids / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D016062:Porosity; D020005:Propanols / Q000737:chemistry / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*; D013997:Time Factors; D014753:Vinyl Compounds / Q000737:chemistry / Q000378:metabolism
D002087:Butyrates; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D052578:Ionic Liquids; D020005:Propanols; D014753:Vinyl Compounds; C095498:vinyl butyrate; D008049:Lipase; C004312:glycidol