title: Statistical optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16.
abstract: The optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16 was conducted using statistical experimental design and analysis. Plackett-Burman design was first used to screen the most important variables influencing hydrogen production, and subsequently central composite design was adopted to investigate the optimum value of the selected factors for achieving maximum hydrogen yield. Experimental results showed that xylose, phosphate buffer, and yeast extract had significant influence on hydrogen production and the maximum hydrogen yield of 2.39 mol/mol xylose was predicted when the concentrations of xylose, phosphate buffer, and yeast extract were 12.24 g/L, 0.170 M, and 4.11 g/L, respectively. The results were further verified by repeated experiments under optimal conditions. The excellent correlation between predicted and measured values further confirmed the validity and practicability of this statistical optimum strategy.
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Guang-li
authors: GL
authors: Cao
authors: Nan-qi
authors: NQ
authors: Ren
authors: Ai-jie
authors: AJ
authors: Wang
authors: Wan-qian
authors: WQ
authors: Guo
authors: Jing
authors: J
authors: Yao
authors: Yu-jie
authors: YJ
authors: Feng
authors: Qing-liang
authors: QL
authors: Zhao
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002021:Buffers; D002478:Cells, Cultured; D002623:Chemistry Techniques, Analytical; D003470:Culture Media / Q000737:chemistry*; D004736:Energy-Generating Resources; D005285:Fermentation; D006859:Hydrogen / Q000737:chemistry*; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D015233:Models, Statistical; D010710:Phosphates / Q000737:chemistry; D046950:Thermoanaerobacterium / Q000378:metabolism*; D014994:Xylose / Q000737:chemistry; D015003:Yeasts / Q000378:metabolism
chemical_list: D002021:Buffers; D003470:Culture Media; D010710:Phosphates; D006859:Hydrogen; D014994:Xylose