Micelle to trapping solution stacking in micellar electrokinetic chromatography.
An analytical strategy micelle to trapping solution stacking (MSS) was developed in acidic buffer in micellar electrokinetic chromatography (MEKC). The stacking mechanism is based on the transport, release, capturing of molecules bound to micelle carriers that are made to collapse into trapping solution (TS) to serve as the medium to contain and stacking the analytes. Tetrandrine and fangchinoline were selected as model mixture using sodium dodecyl sulfate (SDS) micelles as carrier to demonstrate this stacking method. The experiments by MSS-MEKC were carried out and further compared with those by normal MEKC. The results reveal that 113-123-fold improvements in the detection sensitivity was obtained for the analytes, and separation and determination of tetrandrine and fangchinoline in Stephaniae tetrandrae S. Moore and Fengtongan capsules were finished under optimum conditions using the sample matrix containing 8.0mM SDS and TS containing 50mM H(3)PO(4)-55% (v/v) ethanol.
Liu
Lihong
L
School of Pharmaceutical Sciences, Southern Medical University, 1838# North Guangzhou Road, Guangzhou 510515, China. lhliu02@126.com
Deng
Xinxian
X
Chen
Xingguo
X
D044182:Benzylisoquinolines / Q000032:analysis / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D008823:Micelles; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D031609:Stephania tetrandra / Q000737:chemistry*
D044182:Benzylisoquinolines; D008823:Micelles; D010936:Plant Extracts; C009438:tetrandrine; C060802:fangchinoline