First identification of Neospora caninum by PCR in aborted bovine foetuses in Romania.
The first identification of Neospora caninum infection in aborted bovine foetuses in Romania is reported. Nine aborted foetuses were collected from a dairy farm. The foetal age of foetuses was between 3 and 7 months. A 7-month-old foetus was mummified. N. caninum DNA Nc-5 region was amplified from samples extracted from brain tissues of three (33.3%) aborted foetuses. The foetal ages of PCR positive foetuses were 3, 4 and 7 months (mummified). No specific lesions or tissue cysts were found to the histological examination, because of advanced autolysis of brains.
Suteu
Ovidiu
O
Department of Parasitology and Parasitic Diseases, University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Mănăştur Street, Cluj Napoca, Romania.
Titilincu
Adriana
A
Modrý
David
D
Mihalca
Andrei
A
Mircean
Viorica
V
Cozma
Vasile
V
D037881:Aborted Fetus / Q000469:parasitology*; D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012383:Romania
D016054:DNA, Protozoan