A hybrid rhOP-1 delivery system enhances new bone regeneration and consolidation in a rabbit model of distraction osteogenesis.
The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 microg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.
Haidar
Ziyad S
ZS
Division of Orthopaedic Surgery, Shriners Hospital and Montréal Children Hospital, McGill University, Montréal, QC, Canada. ziyad.haidar@mcgill.ca
Tabrizian
Maryam
M
Hamdy
Reggie C
RC
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D016503:Drug Delivery Systems; D006801:Humans; D008297:Male; D023421:Models, Animal; D053758:Nanoparticles; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D019857:Osteogenesis, Distraction / Q000379:methods; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000254:growth & development / Q000601:surgery; D016896:Treatment Outcome
D019485:Bone Morphogenetic Proteins; C511401:osteogenic protein 1 putty, recombinant human