[Human hnRNP I prokaryotic expression and preliminary application.].
OBJECTIVE
To express recombinant protein hnRNP I with prokaryotic expression system and assess the presence of autoantibodies against hnRNP I in systemic sclerosis (SSc) as well as other CTD.


METHODS
Human hnRNP I gene was amplified by RT-PCR from HeLa cells and cloned into pET-30a vector , then pET-30a-hnRNP I plasmid was transferred into E.coli BL21 (DE3) to express recombinant protein. The sera from patients including SSc, SLE, SS, MCTD, UCTD, RA and controls were detected by ELISA with the purified recombinant hnRNP I protein.


RESULTS
The recombinant protein was highly expressed in E.coli BL21(DE3) and specially reacted with clinically diagnosed SSc patients's serum. The result suggested that the autoantibodies against hnRNP I had higher positive ratio(48.72%) in SSc than other serum of AID and control.


CONCLUSIONS
Human hnRNP I protein was successfully expressed in prokaryotic expression system. The purified hnRNP I protein can be used to diagnosis of SSc.
Sun
Cheng-Tao
CT
Department of Immunology, Weifang Medical University; Key Laboratory of Immunology in Universities of Shandong, Weifang 261042, China.
Feng
Fan
F
Wang
Lei
L
Liu
Pei-Hong
PH
Mu
Dong-Zhen
DZ
Ju
Ji-Yu
JY
D001323:Autoantibodies / Q000097:blood; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology
D001323:Autoantibodies; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D011994:Recombinant Proteins