title: Supercritical fluid extraction and high performance liquid chromatographic determination of benzopyrans and phloroglucinol derivative in Hypericum polyanthemum.
abstract: The aerial parts of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferae) were successively extracted with supercritical carbon dioxide (SC CO(2)) under pressures of 90, 120, 150 and 200 bar at different temperatures (40, 50 and 60 degrees C), and compared with the n-hexane extract obtained by ultrasound-assisted extraction. The samples obtained were examined regarding extraction yield and HPLC quantification of the main secondary metabolites, the benzopyrans HP1 (6-isobutyryl-5,7-dimethoxy-2,2-dimethylbenzopyran), HP2 (7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran) and HP3 (5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl) and the phloroglucinol derivative, uliginosin B. SFE presented higher selectivity than the n-hexane maceration, and the best condition to extract the target metabolites has been determined to be at 50 degrees C and for the high molecular-weight compound, uliginosin B, higher pressures were required.
authors: Programa de Pós Graduação em Ciências Farmacêuticas, UFRGS. Av. Ipiranga, 2752, 90610.000 Porto Alegre, RS, Brazil.
authors: Simone Tasca
authors: ST
authors: Cargnin
authors: Jéssica
authors: J
authors: de Matos Nunes
authors: Juliana Schulte
authors: JS
authors: Haas
authors: Luís Fernando
authors: LF
authors: Baladão
authors: Eduardo
authors: E
authors: Cassel
authors: Rubem Figueiró
authors: RF
authors: Vargas
authors: Sandra Beatriz
authors: SB
authors: Rech
authors: Gilsane Lino
authors: GL
authors: von Poser
mesh_terms: D000704:Analysis of Variance; D001578:Benzopyrans / Q000032:analysis* / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D006586:Hexanes / Q000737:chemistry; D020902:Hypericum / Q000737:chemistry*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D001578:Benzopyrans; D006586:Hexanes; D002245:Carbon Dioxide; C026385:n-hexane; D010696:Phloroglucinol; C547933:uliginosin B