A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae.
A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.
Imperi
Monica
M
Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, 00161 Rome, Italy.
Pataracchia
Marco
M
Alfarone
Giovanna
G
Baldassarri
Lucilla
L
Orefici
Graziella
G
Creti
Roberta
R
D016667:Bacterial Capsules / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial