title: Expression of genes for appetite-regulating peptides in the hypothalamus of genetically selected lean and fat sheep.
abstract: OBJECTIVE
  The aim was to determine whether genetic selection of sheep for body composition could be accounted for by changes in the level of expression of genes for appetite-regulating peptides in the hypothalamus. We examined gene expression in the hypothalamus of genetically lean, normal and fat ewes (n = 5/group).
  
  
  METHODS
  Plasma growth hormone (GH) and metabolic indicators were measured and gene expression in brains was quantified by in situ hybridization.
  
  
  RESULTS
  Body weight and voluntary food intake (VFI) were similar between groups, but lean and fat animals respectively had low and high indices of adiposity. GH levels were higher in lean and fat animals than in controls. In the arcuate nucleus (ARC), neuropeptide Y (NPY) expression/cell was higher in the lean animals than in normal animals, but overall NPY expression was similar in fat and normal animals. Pro-opiomelanocortin (POMC) and leptin receptor (ObRb) expression in the ARC was similar across groups. Orexin (ORX) and melanin-concentrating hormone (MCH) expression was inversely correlated to adiposity, being higher in lean and lower in fat animals.
  
  
  CONCLUSIONS
  Expression of genes for orexigenic neuropeptides is altered in a consistent way. Energy expenditure is reduced by MCH but increased by ORX, so increased expression of the latter may cause increased energy expenditure in the lean animals and vice versa in the fat animals.
authors: Department of Physiology, Monash University, Clayton, VIC 3800, Australia.
authors: Chantacha
authors: C
authors: Anukulkitch
authors: Alexandra
authors: A
authors: Rao
authors: Alda
authors: A
authors: Pereira
authors: John
authors: J
authors: McEwan
authors: Iain J
authors: IJ
authors: Clarke
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000818:Animals; D001066:Appetite / Q000235:genetics*; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D001835:Body Weight / Q000235:genetics; D004435:Eating / Q000235:genetics; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000097:blood*; D007028:Hypothalamic Hormones / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D000068797:Orexins; D010907:Pituitary Hormones / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D054411:Receptors, Leptin / Q000378:metabolism; D012641:Selection, Genetic; D012756:Sheep
chemical_list: D007028:Hypothalamic Hormones; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009478:Neuropeptide Y; D009479:Neuropeptides; D000068797:Orexins; D010907:Pituitary Hormones; D054411:Receptors, Leptin; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone; D013006:Growth Hormone