Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.
A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.
Campelo
Ana Belén
AB
Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Apdo. 85, 33300 Villaviciosa, Asturias, Spain.
Rodríguez
Ana
A
Martínez
Beatriz
B
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005285:Fermentation / Q000235:genetics; D005456:Fluorescent Dyes / Q000032:analysis*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D007218:Industrial Microbiology; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D050296:Microbial Viability / Q000235:genetics; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins