Comparing the effects of acupuncture and electroacupuncture at Zusanli and Baihui on cell proliferation and neuroblast differentiation in the rat hippocampus.
We compared the effects of acupuncture and electroacupuncture on cell proliferation and neuroblast differentiation using specific markers, Ki67 and doublecortin (DCX), in the subgranular zone of the dentate gyrus (SZDG) in 13-week old Wistar rats. Acupuncture and electroacupuncture were applied simultaneously in the acu-points, ST36 (Zusanli) and GV20 (Baihui), once a day for 3 weeks. Acupuncture and electroacupuncture at these acu-points significantly increased the number of Ki67-positive cells and DCX-immunoreactive neuroblasts compared to the control or sham acupuncture group. Electroacupuncture treatment significantly increased the number of well-developed (tertiary) dendrites in the SZDG compared to acupuncture treatment. These results suggest that both acupuncture and electroacupuncture increase neurogenesis in the normal, but that electroacupuncture has greater effects on neuroblast plasticity than acupuncture in the dentate gyrus.
Hwang
In Koo
IK
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Chung
Jin Young
JY
Yoo
Dae Young
DY
Yi
Sun Shin
SS
Youn
Hwa Young
HY
Seong
Je Kyung
JK
Yoon
Yeo Sung
YS
D026881:Acupuncture / Q000379:methods*; D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D002545:Brain Ischemia / Q000628:therapy; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology; D000089662:Doublecortin Protein; D015671:Electroacupuncture / Q000379:methods*; D004827:Epilepsy / Q000628:therapy; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen