[Effects of dexamethasone on expression of Th17 transcription factor RORgammat mRNA in experimental asthma mice.].
OBJECTIVE
To study the effects of dexamethasone (Dex) on expression of Th17 transcription factor retinoic acid-related orphan receptor gamma t(RORgammat)in asthma.


METHODS
The BALB/c mice asthma model was induced by ovalbumin(OVA) with classic method.Thirty female mice were randomly divided into control group, asthmatic group and Dex treated group. The level of IL-17 in mice bronchoalveolar lavage fluid (BALF) and serum was measured by enzyme-linked immunosorbent assay(ELISA). Airway Responsiveness to acetylcholine chloride(Ach) was measured by a modified non-invasive method; The airway inflammation was evaluated by HE staining.The expression of RORgammat mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).


RESULTS
The level of RORgammat mRNA, IL-17 of asthmatic group were significantly higher than those of control group(P<0.01), which were significantly reduced by Dex compared with the asthmatic group(P<0.05).


CONCLUSIONS
Dex can inhibit the release of IL-17, whose mechanism may be the blockade of TH17 differentiation in asthmatic models by inhibit the RORgammat expression.
Hu
Si-Ming
SM
Department of Respiratory Diseases, Nangfan Hospital, Southern Medical University, Guangzhou 510515, China.
Luo
Ya-Ling
YL
Lai
Wen-Yan
WY
D000818:Animals; D001249:Asthma / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors
D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger; D014157:Transcription Factors; D003907:Dexamethasone