title: Quantifying the biological significance of gene ontology biological processes--implications for the analysis of systems-wide data.
abstract: BACKGROUND
  Gene Ontology (GO), the de facto standard for representing protein functional aspects, is being used beyond the primary goal for which it is designed: protein functional annotation. It is increasingly used to evaluate large sets of relationships between proteins, e.g. protein-protein interactions or mRNA co-expression, under the assumption that related proteins tend to have the same or similar GO terms. Nevertheless, this assumption only holds for terms representing functional groups with biological significance ('classes'), and not for the ones representing human-imposed aggregations or conceptualizations lacking a biological rationale ('categories').
  
  
  RESULTS
  Using a data-driven approach based on a set of high-quality functional associations, we quantify the functional coherence of GO biological process (GO:BP) terms as well as their explicit and implicit relationships, trying to distinguish classes and categories. We show that the quantification used is in agreement with the distinction one would intuitively make between these two concepts. As not all GO:BP terms and relationships are equally supported by current functional associations, any detailed validation of new experimental data using GO:BP, beyond whole-system statistics, should take such unbalance into account.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors: Computational Systems Biology Group, National Centre for Biotechnology (CNB-CSIC), C/ Darwin 3, 28049 Madrid, Spain.
authors: Monica
authors: M
authors: Chagoyen
authors: Florencio
authors: F
authors: Pazos
mesh_terms: D001686:Biological Phenomena; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D020869:Gene Expression Profiling / Q000379:methods; D025941:Protein Interaction Mapping; D011506:Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D011506:Proteins; D012333:RNA, Messenger