Suppression of proinflammatory cytokine production by specific metabolites of Lactobacillus plantarum 10hk2 via inhibiting NF-κB and p38 MAPK expressions.
The objective of this study was to evaluate the immunomodulatory effects of specific bacterial metabolites of Lactobacillus plantarum 10hk2 to induce anti-inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264.7. The effects of the extracellular metabolites of this bacterial strain were examined by dividing them into protein and polysaccharide fractions. A specific protein fraction (8.7 kDa) was found to be a strong IL-10 inducer in LPS-stimulated RAW 264.7 cells and suppressed LPS-induced NF-κB induction and inhibited LPS-induced phosphorylation of I-κB and p38 MAPK. To the best of our knowledge, this was the first study that investigated the anti-inflammatory effects of an extracellular peptide derived from lactic acid bacteria. In addition, we characterized the inhibitory mode of this molecule in the induction of proinflammatory cytokines. Based on the findings presented in this study, this molecule holds promise for use as an agent to modulate inflammation related diseases.
Chon
Heeson
H
R&D Center, Milae Resources ML Co., Ltd., 7FL, #24-3 Bangi-dong, Songpa-gu, Seoul 138-050, Korea. kini132@empal.com
Choi
Byungryul
B
Jeong
Gajin
G
Lee
Eungyo
E
Lee
Seunghui
S
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000187:drug effects; D020672:I-kappa B Proteins / Q000378:metabolism; D056747:Immunomodulation; D018836:Inflammation Mediators / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000032:analysis; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D001426:Bacterial Proteins; D016207:Cytokines; D020672:I-kappa B Proteins; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010455:Peptides; D011135:Polysaccharides, Bacterial; D016753:Interleukin-10; D009569:Nitric Oxide; D048051:p38 Mitogen-Activated Protein Kinases