title: A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae.
abstract: A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.
authors: Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Monica
authors: M
authors: Imperi
authors: Marco
authors: M
authors: Pataracchia
authors: Giovanna
authors: G
authors: Alfarone
authors: Lucilla
authors: L
authors: Baldassarri
authors: Graziella
authors: G
authors: Orefici
authors: Roberta
authors: R
authors: Creti
mesh_terms: D016667:Bacterial Capsules / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial