[Regulatory effects of Wuzhuyutang (Evodiae prescription) and its consisting herbs on TPH2 promoter].
OBJECTIVE
To screen the active component of Wuzhuyutang (WZYT, Evodiae prescription) and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine.


METHODS
By transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated.


RESULTS
Different regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed.


CONCLUSIONS
TPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.
Wang
Yugang
Y
Laboratory of Pharmaceutics, Department of Biological Sciences and Biotechnology, Tsignghua University, Beijng 100084, China.
Lei
Fan
F
Wang
Xiukun
X
Hu
Jun
J
Zhan
Honglei
H
Xing
Dongming
D
Du
Lijun
L
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D032086:Evodia / Q000737:chemistry*; D006801:Humans; D008881:Migraine Disorders / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D016716:PC12 Cells; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D014365:Tryptophan Hydroxylase / Q000235:genetics* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D014365:Tryptophan Hydroxylase; C504569:tph2 protein, rat