[Determination of militarine in Rhizoma Bletillae by HPLC].
OBJECTIVE
To develop an HPLC method for determination of militarine in Rhizoma Bletillae.


METHODS
Analysis was carried out on a Symmetry C18 analytical column, and a C18 guard column eluted with mobile phase consisted of acetonitrile and water (37:63) militarine the detection wavelength was set at 244 nm.


RESULTS
The contents of in 12 samples of Rhizoma Bletillae in market were in the ranges of 1.45%-3.32%.


CONCLUSIONS
The method is simple, accurate and reproducible, and is suitable for the quality control of Rhizoma Bletillae.
He
Xun
X
School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
Wang
Aimin
A
Li
Yongjun
Y
Wang
Yonglin
Y
Zhou
Shuyong
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D029595:Orchidaceae / Q000737:chemistry*; D013386:Succinates / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D013386:Succinates; C545907:militarine