title: [Study on absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model].
abstract: OBJECTIVE
  To study the absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model.
  
  
  METHODS
  The Caco-2 monolayer model was used to explore the effect of time and drug concentration on the absorption of puerarin and its nanoparticeles. The drug concentration was determined by high-performance liquid chromatography and the Papp was caculated.
  
  
  RESULTS
  The puerarin-PLGA-NP could significantly improve the absorption of puerarin. Puerarin was not only absorbed simply through passive diffusion, but also had relations with high dispersion of nanoparticles, increase of affinity and interaction with intestinal mucous membrane.
  
  
  CONCLUSIONS
  The main mechanism of puerarin and its nanoparticles across Caco-2 monolayer model is passive transference. Puerarin-NP could increase the absorption of the drug in intestinal epithlial.
authors: Research Institute of Qinghua University in Shenzhen, Shenzhen 518057, China. lxj_1975@yahoo.com.cn
authors: Xi-Jing
authors: XJ
authors: Liu
authors: Qiang
authors: Q
authors: Jia
authors: Cheng-Yuan
authors: CY
authors: Wang
authors: Nai-Li
authors: NL
authors: Wang
mesh_terms: D000042:Absorption; D001692:Biological Transport; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000493:pharmacokinetics*; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D011091:Polyesters; C033607:puerarin