title: Rapid screening of antibody aggregates and protein impurities using short gel filtration columns.
abstract: Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity, and the multimeric state of a protein of interest. Superdex 200 and Superdex 75 are outstanding GF media for such analysis. To speed up analysis and keep sample and buffer consumption at minimum, two prepacked short GF columns have been developed, Superdex 200 5/150 GL and Superdex 75 5/150 GL. With lengths of only 15 cm and volume of 3 ml, these columns allow rapid analysis (6-12 min/run) with minimal sample (4-50microl) and buffer consumption.
authors: GE Healthcare Bio-Sciences AB, Uppsala, Sweden.
authors: Inger
authors: I
authors: Salomonson
authors: Jinyu
authors: J
authors: Zou
authors: Marianne
authors: M
authors: Carlsson
authors: Ann
authors: A
authors: Bergh
authors: Fredrik
authors: F
authors: Lundström
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D002021:Buffers; D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000737:chemistry; D013997:Time Factors
chemical_list: D000906:Antibodies; D002021:Buffers; D011506:Proteins