Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia.
Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding gamma-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
Kang
Kyungpil
K
Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine, Seoul 120-752, Korea.
Kim
Juri
J
Yong
Tai-Soon
TS
Park
Soon-Jung
SJ
D000818:Animals; D003598:Cytoskeletal Proteins / Q000302:isolation & purification / Q000378:metabolism; D003599:Cytoskeleton / Q000737:chemistry; D016829:Giardia lamblia / Q000737:chemistry / Q000502:physiology*; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015800:Protozoan Proteins / Q000302:isolation & purification / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D003598:Cytoskeletal Proteins; C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia