[Study of specificity of fungistatic effect of trichothecene mycotoxins].
In attempt to realize the purposeful search of highly sensitive and highly specific microbial test-cultures the authors studied the ratio between selected before species of yeast to the wide set of different mycotoxins including macrocyclic trichothecenes (T-2 toxin, HT-2 toxin, T-2 triol, T-2 tetraol, NT-1 toxin, NT-2 toxin, fusarenon-X, diacetoxyscirpenol, deoxynivalenol, trichothecin, trichothecolon, neosolaniol, verrucarin A, roridin A, roridin H, aflatoxins, B1, B2, G1 and G2, sterigmatocystin, patulin, fusaric acid, ochratoxin A, zearalenon and their derivatives). It was supposed to define borders of specific sensitivity of test-cultures. The value of minimal inhibiting concentration (MIC) of mycotoxins, which caused fungicide action in respect of the investigated yeast strains, was used as the estimation criterion. Extremely few species with narrow specific sensitivity to individual trichothecenes were found. Strains with group sensitivity to investigated trichothecene mycotoxins (MCTC) were more widely presented. Selectivity in respect of either simple (T-2 toxin), or MCTC (verrucarin A, roridin A, roridin H) is traced for some species. Taxons with mixed selectivity were discovered. Exceptionally high fungistatic activity was characteristic of the investigated MCTC, T-2 toxin, trichothecin. Values of MCTC for these substances in respect of more sensitive strains varied in the borders of 0.03-0.1; 0.1-1.0 and 0.02-0.1 mcg/disk, correspondingly. It was less expressed for diacetoxyscirpenol, deoxynivalenol, trichothecolon (0.8-5.0; 0.1-2.0 and 0.8-4.0 mcg/disk, correspondingly). The data obtained give reasons for completing the bank of yeast test-cultures with wide potentialities of mycotoxins indication in various food and fodder substrates.
Zaĭchenko
A M
AM
Nagornaia
S S
SS
Andrienko
E V
EV
Tsyganenko
E S
ES
D001681:Biological Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D008826:Microbial Sensitivity Tests; D009183:Mycotoxins / Q000737:chemistry / Q000633:toxicity; D013329:Structure-Activity Relationship; D014255:Trichothecenes / Q000737:chemistry / Q000633:toxicity*; D015003:Yeasts / Q000187:drug effects*
D009183:Mycotoxins; D014255:Trichothecenes