Two tachykinin-like peptides from skin secretions of Danio rerio.
Tachykinin perform multiple physiological functions such as smoothing muscle contraction, vasodilation, inflammation, the processing of nerve signal, neuroprotection and neurodegeneration. Two novel tachykinin-like peptides named tachykinin-DR1 and -DR2 were identified from skin secretions of Danio rerio in current work. Their amino acid sequences were determined as SKSQHFHGLM-NH(2) and NKGEIFVGLM-NH(2), respectively. They share a conserved FXGLM-NH(2)C-terminal consensus motif. By cDNA cloning, the precursor encoding both tachykinin-DR1 and -DR2 was screened from the skin cDNA library of D. rerio. Tachykinin-DR1 and -DR2 share the same precursor, which is composed of 108 amino acid (aa) residues. Regarding the biological activity, tachykinin-DRs could induce the contraction of isolated strips of guinea pig ileum just like other tackykinins. To our best knowledge, this is the first report of tachykinin from fish skin.
Mi
Xuhua
X
Department of Nephrology, West China Hospital of Sichuan University, Chengdu, Sichuan, 610041 China.
Yu
Haining
H
Jia
Peng
P
Zhang
Zhenzhou
Z
Zhang
Luyong
L
Liu
Jingze
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D010455:Peptides; D012867:Skin / Q000737:chemistry*; D015320:Tachykinins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
D018377:Neurotransmitter Agents; D010455:Peptides; D015320:Tachykinins; D029961:Zebrafish Proteins