title: Low-calorie diet-induced reduction in serum HDL cholesterol is ameliorated in obese women with the -3826 G allele in the uncoupling protein-1 gene.
abstract: The uncoupling protein-1 (UCP1) gene is of major importance for regulation of body weight and lipid/lipoprotein metabolism. Our cross-sectional study has shown that subjects with the G/G genotype of the -3826 A/G polymorphism in the UCP-1 gene have higher levels of serum high-density lipoprotein cholesterol (HDL-C) than those with other genotypes. Low circulating HDL-C level has been regarded as a major atherosclerotic risk factor. We therefore investigated whether the -3826 A/G polymorphism affects the obesity- and lipid-related parameters during a low-calorie diet (LCD) intervention. In 32 obese women (49.9 +/- 8.4 years of age), anthropometric, physiological and biochemical characteristics were measured before and after a 2-month LCD treatment, which restricted each subject to the same energy intakes, such as 5,120 kJ/day. The -3826 A/G polymorphism was detected using a PCR-restriction fragment-length polymorphism method. There were 6 subjects with the A/A genotype, 15 with the A/G genotype and 11 with the G/G genotype. The LCD intervention decreased weight (P < 0.001) and serum HDL-C levels (P < 0.05) in all subjects. There was no difference in the levels of change in weight, nutrient intake, physiological measurements in energy expenditure, and fat oxidation between subjects with and without the G allele. In contrast, the degree of the reduction in the HDL-C levels was significantly smaller in subjects with the G allele than those without the G allele. These results suggest that the G allele at -3826 in the UCP1 gene may ameliorate the reduction in serum HDL-C levels in obese women during LCD.
authors: Graduate School of Sport and Exercise Science, Osaka University of Health and Sport Sciences, Osaka, Japan. hamada@ouhs.ac.jp
authors: Taku
authors: T
authors: Hamada
authors: Kazuhiko
authors: K
authors: Kotani
authors: Narumi
authors: N
authors: Nagai
authors: Kokoro
authors: K
authors: Tsuzaki
authors: Yukiyo
authors: Y
authors: Matsuoka
authors: Yoshiko
authors: Y
authors: Sano
authors: Mami
authors: M
authors: Fujibayashi
authors: Natsuki
authors: N
authors: Kiyohara
authors: Seitaro
authors: S
authors: Tanaka
authors: Makiko
authors: M
authors: Yoshimura
authors: Kahori
authors: K
authors: Egawa
authors: Yoshinori
authors: Y
authors: Kitagawa
authors: Yoshinobu
authors: Y
authors: Kiso
authors: Toshio
authors: T
authors: Moritani
authors: Naoki
authors: N
authors: Sakane
mesh_terms: D000483:Alleles; D008076:Cholesterol, HDL / Q000097:blood*; D004038:Diet, Reducing; D005260:Female; D005838:Genotype; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000097:blood* / Q000178:diet therapy* / Q000235:genetics; D000071256:Uncoupling Protein 1
chemical_list: D008076:Cholesterol, HDL; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1