Cyclosporine a-loaded solid lipid nanoparticles: ocular tolerance and in vivo drug release in rabbit eyes.
OBJECTIVE
To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes.


METHODS
SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing.


RESULTS
The particle size of SLNs was detected as 225.9 +/- 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes.


CONCLUSIONS
Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.
Gökçe
Evren H
EH
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, Turkey. evren.homan.gokce@ege.edu.tr
Sandri
Giuseppina
G
Eğrilmez
Sait
S
Bonferoni
M Cristina
MC
Güneri
Tamer
T
Caramella
Carla
C
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001682:Biological Availability; D001693:Biological Transport, Active; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D008055:Lipids / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011817:Rabbits
D007166:Immunosuppressive Agents; D008055:Lipids; D016572:Cyclosporine