High expression of macrophage migration inhibitory factor in the murine lacrimal gland.
OBJECTIVE
To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands.


METHODS
Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay.


RESULTS
Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription-polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of beta-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera.


CONCLUSIONS
The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.
Park
Choul Yong
CY
Department of Ophthalmology, Johns Hopkins University, Baltimore, MD 21117, USA.
Shin
Young Joo
YJ
Lekhanont
Kaevalin
K
Zhang
Cheng
C
Lee
Woo-Seok
WS
Cano
Marisol
M
Bucala
Richard
R
Chuck
Roy S
RS
D000818:Animals; D005128:Eye Diseases / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D007765:Lacrimal Apparatus / Q000378:metabolism* / Q000473:pathology; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger; D019746:Intramolecular Oxidoreductases; C076231:Mif protein, mouse