Acafusin, a dimeric antifungal protein from Acacia confusa seeds.
A dimeric 34-kDa antifungal protein, designated as acafusin, was purified from Acacia confusa seeds using Q-Sepharose, DEAE-cellulose, Mono S and Superdex S75. It demonstrated antifungal activity toward Rhizoctonia solani with an IC(50) of 28 microM. Acafusin inhibited the activity of HIV-1 reverse transcriptase mildly with an IC(50) of 80 microM.
Lam
Sze Kwan
SK
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Ng
Tzi Bun
TB
D000045:Acacia / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors; D012232:Rhizoctonia / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013696:Temperature
D000935:Antifungal Agents; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D002686:Chitin; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase