title: The role of rhodopsin glycosylation in protein folding, trafficking, and light-sensitive retinal degeneration.
abstract: Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. Here we examined the role of rhodopsin glycosylation in biosynthesis, trafficking, and retinal degeneration (RD) using transgenic Xenopus laevis expressing glycosylation-defective human rhodopsin mutants. Although mutations T4K and T4N caused RD, N2S and T4V did not, demonstrating that glycosylation at N2 was not required for photoreceptor viability. In contrast, similar mutations eliminating glycosylation at N15 (N15S and T17M) caused rod death. Expression of T17M was more toxic than T4K to transgenic photoreceptors, further suggesting that glycosylation at N15 plays a more important physiological role than glycosylation at N2. Together, these results indicate that the structure of the rhodopsin N terminus must be maintained by an appropriate amino acid sequence surrounding N2 and may require a carbohydrate moiety at N15. The mutant rhodopsins were rendered less toxic in their dark inactive states, because RD was abolished or significantly reduced when transgenic tadpoles expressing T4K, T17M, and N2S/N15S were protected from light exposure. Regardless of their effect on rod viability, all of the mutants primarily localized to the outer segment and Golgi and showed little or no endoplasmic reticulum accumulation. Thus, glycosylation was not crucial for rhodopsin biosynthesis or trafficking. Interestingly, expression of similar bovine rhodopsin mutants did not cause rod cell death, possibly attributable to greater stability of bovine rhodopsin.
authors: Department of Ophthalmology and Visual Sciences and Centre for Macular Research, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Beatrice M
authors: BM
authors: Tam
authors: Orson L
authors: OL
authors: Moritz
mesh_terms: D000596:Amino Acids / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D006031:Glycosylation; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007814:Larva; D008027:Light / Q000009:adverse effects*; D009154:Mutation / Q000235:genetics; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism; D017510:Protein Folding; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus
chemical_list: D000596:Amino Acids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012243:Rhodopsin