Laminarin-mediated targeting to Dectin-1 enhances antigen-specific immune responses.
It has immense potential for immunotherapy and vaccination to target antigens to antigen-presenting cells (APCs). Here we described a method for delivering whole protein antigens to APCs via carbohydrate-mediated targeting of Dectin-1, which is a C-type lectin and mainly expresses on subpopulations of dendritic cells and macrophages. Laminarin, which is a beta-1-3 glucan and a typical ligand for Dectin-1, was chemically coupled to ovalbumin (OVA). Compared to OVA alone, the conjugate was effectively recognized and ingested by CHO cells stably expressing Dectin-1 and bound to bone marrow dendritic cells (BMDCs) via Dectin-1. Laminarin modification led to significant enhancement of OVA-specific CD4(+) T-cell response. Moreover, when used to immunize mice, the conjugate enhanced the primary IgG antibody response to OVA. Taken together, our data suggest that APCs targeting based on glucan-Dectin-1 interaction is a promising approach to improve vaccines.
Xie
Jianhui
J
Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, PR China.
Guo
Liang
L
Ruan
Yuanyuan
Y
Zhu
Haiyan
H
Wang
Lan
L
Zhou
Lei
L
Yun
Xiaojing
X
Gu
Jianxin
J
D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003713:Dendritic Cells / Q000276:immunology; D005936:Glucans; D037181:Lectins, C-Type / Q000737:chemistry / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology*; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D014612:Vaccines / Q000737:chemistry / Q000276:immunology*; D047071:beta-Glucans / Q000737:chemistry / Q000276:immunology; D000099181:Dectin-1
D000941:Antigens; D005936:Glucans; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010047:Ovalbumin; D011134:Polysaccharides; D014612:Vaccines; D047071:beta-Glucans; D000099181:Dectin-1; C008247:laminaran; C033363:beta-1,3-glucan