title: Single nucleotide polymorphisms in a gene for translation initiation factor (eIF4G) of rice (Oryza sativa) associated with resistance to Rice tungro spherical virus.
abstract: Rice tungro disease (RTD) is a serious constraint to rice production in South and Southeast Asia. RTD is caused by Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Rice cv. Utri Merah is resistant to RTSV. To identify the gene or genes involved in RTSV resistance, the association of genotypic and phenotypic variations for RTSV resistance was examined in backcross populations derived from Utri Merah and rice germplasm with known RTSV resistance. Genetic analysis revealed that resistance to RTSV in Utri Merah was controlled by a single recessive gene (tsv1) mapped within an approximately 200-kb region between 22.05 and 22.25 Mb of chromosome 7. A gene for putative translation initiation factor 4G (eIF4G(tsv1)) was found in the tsv1 region. Comparison of eIF4G(tsv1) gene sequences among susceptible and resistant plants suggested the association of RTSV resistance with one of the single nucleotide polymorphism (SNP) sites found in exon 9 of the gene. Examination of the SNP site in the eIF4G(tsv1) gene among various rice plants resistant and susceptible to RTSV corroborated the association of SNP or deletions in codons for Val(1060-1061) of the predicted eIF4G(tsv1) with RTSV resistance in rice.
authors: Department of Functional Crop Science, National Institute of Crop Sciences, Rural Development Administration, 1085 Neidong, Milyang, Gyeongnam, 627-803, Republic of Korea.
authors: Jong-Hee
authors: JH
authors: Lee
authors: Muhammad
authors: M
authors: Muhsin
authors: Genelou A
authors: GA
authors: Atienza
authors: Do-Yeon
authors: DY
authors: Kwak
authors: Suk-Man
authors: SM
authors: Kim
authors: Teresa B
authors: TB
authors: De Leon
authors: Enrique R
authors: ER
authors: Angeles
authors: Edgardo
authors: E
authors: Coloquio
authors: Hiroaki
authors: H
authors: Kondoh
authors: Kouji
authors: K
authors: Satoh
authors: Rogelio C
authors: RC
authors: Cabunagan
authors: Pepito Q
authors: PQ
authors: Cabauatan
authors: Shoshi
authors: S
authors: Kikuchi
authors: Hei
authors: H
authors: Leung
authors: Il-Ryong
authors: IR
authors: Choi
mesh_terms: D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics* / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics; D005808:Genes, Recessive / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000821:virology; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016415:Sequence Alignment; D019193:Waikavirus / Q000502:physiology*
chemical_list: D039603:Eukaryotic Initiation Factor-4G