title: Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?
abstract: The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. The results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. In ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. In conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones.
authors: Department of Psychobiology, Universidade Federal de São Paulo, Vila Clementino, SP, São Paulo, Brazil. mandersen@psicobio.epm.br
authors: Monica L
authors: ML
authors: Andersen
authors: Daniel A
authors: DA
authors: Ribeiro
authors: Tathiana A
authors: TA
authors: Alvarenga
authors: Andressa
authors: A
authors: Silva
authors: Paula
authors: P
authors: Araujo
authors: Adriano
authors: A
authors: Zager
authors: Neuli M
authors: NM
authors: Tenorio
authors: Sergio
authors: S
authors: Tufik
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism; D004249:DNA Damage; D004031:Diestrus / Q000235:genetics / Q000502:physiology; D004971:Estrus / Q000235:genetics / Q000502:physiology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010052:Ovariectomy; D011359:Proestrus / Q000235:genetics / Q000502:physiology; D011374:Progesterone / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000235:genetics* / Q000378:metabolism*; D012895:Sleep, REM; D013739:Testosterone / Q000378:metabolism
chemical_list: D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D003345:Corticosterone