Evaluation of two immunoblot assays and a Western blot assay for the detection of antisyphilis immunoglobulin g antibodies.
In the present study, two immunoglobulin G (IgG) immunoblot assays and one IgG Western blot assay were compared to the rapid plasma reagin test (RPR), the fluorescent treponemal antibody absorption test (FTA-ABS), and the Treponema pallidum particle agglutination assay (TP-PA). The agreement levels of the Viramed, Virotech, and MarDx assays were 97.0%, 96.4%, and 99.4%, and the agreements of samples inconclusive by FTA-ABS and resolved by TP-PA were 91.7%, 83.3%, and 69.4%, respectively.
Welch
Ryan J
RJ
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA. ryan.welch@aruplab.com
Litwin
Christine M
CM
D000907:Antibodies, Bacterial / Q000097:blood*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013587:Syphilis / Q000175:diagnosis*; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G