Protein oxidation biomarkers in plasma of type 2 diabetic patients.
OBJECTIVE
To evaluate oxidative stress and the extent of oxidation of plasma proteins in type 2 diabetic patients.


METHODS
Study was carried out on blood from 31 diabetic patients of both sexes (mean age = 58 + or - 7; duration of diabetes 12 + or - 5 years) and healthy age and sex matched normal subjects. Biomarkers of protein oxidation; plasma protein carbonyls (PCO), advanced oxidation protein products (AOPPs) and -SH group and free radical scavenging capacity of plasma was measured.


RESULTS
PCO and AOPPS levels were significantly (P<0.005) higher in diabetic patients in comparison to healthy volunteers. Reduced free radical scavenging capacity (P<0.001) and -SH group (P<0.05) was observed in plasma of type 2 diabetic patients.


CONCLUSIONS
Our data suggest that diabetics are susceptible to protein oxidation. Oxidative modulation of proteins due to reduced radical scavenging activity of plasma patients may be one of the reasons of altered physiological processes in type 2 diabetic patients.
Pandey
Kanti Bhooshan
KB
Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Mishra
Neetu
N
Rizvi
Syed Ibrahim
SI
D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D050050:Protein Carbonylation
D015415:Biomarkers; D001798:Blood Proteins; D016166:Free Radical Scavengers