[Investigation on the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell degranulation.].
OBJECTIVE
To investigate the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell release and search for the suitable index to describe mast cell degranulation.


METHODS
Mast cell degranulation was performed in vitro, in which histamine, tryptase and beta-hexosaminidase are detected by spectrophotometry.


RESULTS
The concordance was found to exit among the three mediums, furthermore the study discloses that more similarity was found between tryptase and beta-hexosaminidase. Histamine was difficult and inconstant to be detected.


CONCLUSIONS
Tryptase and beta-hexosaminidase are more suitable and accurately to reflect mast cell degranulation in vitro than histamine. Tryptase is the excellent index to detect mast cell degranulation in vitro.
Guo
Yong-Chao
YC
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Li
Zhen-Xing
ZX
Lin
Hong
H
D015550:Cell Degranulation; D006632:Histamine; D006636:Histamine Release; D006801:Humans; D008407:Mast Cells / Q000276:immunology; D012697:Serine Endopeptidases; D053802:Tryptases; D001619:beta-N-Acetylhexosaminidases
D006632:Histamine; D001619:beta-N-Acetylhexosaminidases; D012697:Serine Endopeptidases; D053802:Tryptases