title: Widefield fluorescence sectioning with HiLo microscopy.
abstract: HiLo microscopy is a widefield fluorescence imaging technique that provides depth discrimination by combining two images, one with non-uniform illumination and one with uniform illumination. We discuss the theory of this technique and a variety of practical implementations in brain-tissue imaging and fluorescence endomicroscopy.
authors: Boston University, Department of Biomedical Engineering, 44 Cummington St., Boston, MA, 02215, USA. jmertz@bu.edu
authors: Jerome
authors: J
authors: Mertz
authors: Daryl
authors: D
authors: Lim
authors: Kengyeh K
authors: KK
authors: Chu
authors: Nenad
authors: N
authors: Bozinovic
authors: Timothy
authors: T
authors: Ford
mesh_terms: D000818:Animals; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D015203:Reproducibility of Results