title: Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells.
abstract: OBJECTIVE
  In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4(+) CD25(+) regulatory T (Treg) cells, in order to inform further studies in Treg cell function.
  
  
  METHODS
  We separately used magnetic cell sorting and flow cytometry sorting to identify CD4(+) CD25(+) Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4(+) CD25(+) Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4(+) CD25(+) Treg cells in the in vitro proliferation experiments.
  
  
  RESULTS
  The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4(+) CD25(+) Treg cells, however, obtained with both methods have similar immunosuppressive capacities.
  
  
  CONCLUSIONS
  The result suggests that both methods can be used in isolating CD4(+) CD25(+) Treg cells, and one can select the best method according to specific needs and availability of the methodologies.
authors: Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medicine College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Hang
authors: H
authors: Yan
authors: Chen-guang
authors: CG
authors: Ding
authors: Pu-xun
authors: PX
authors: Tian
authors: Guan-qun
authors: GQ
authors: Ge
authors: Zhan-kui
authors: ZK
authors: Jin
authors: Li-ning
authors: LN
authors: Jia
authors: Xiao-ming
authors: XM
authors: Ding
authors: Xiao-ming
authors: XM
authors: Pan
authors: Wu-jun
authors: WJ
authors: Xue
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002469:Cell Separation; D002470:Cell Survival; D005434:Flow Cytometry / Q000379:methods*; D007107:Immune System; D007166:Immunosuppressive Agents / Q000494:pharmacology; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D007166:Immunosuppressive Agents; D053645:Interleukin-2 Receptor alpha Subunit