Effects of BMP-2 and dexamethasone on osteogenic differentiation of rat dental follicle progenitor cells seeded on three-dimensional beta-TCP.
The aim of this study was to investigate the effects of BMP-2 and dexamethasone (Dex) on osteogenic differentiation of rat dental follicle progenitor cells (RDFCs) seeded on three-dimensional beta-TCP. The alkaline phosphatase (ALP), the calcium and phosphonium, the osteocalcin in media of the third passage RDFCs on biomaterial beta-TCP after 1-3, 3-7, 7-14 days of culture were examined respectively. The growth of cells on the scaffolds was observed by scanning electron microscope (SEM) after 3, 7 days of culture and by implanting in the backs of severe combined immunodeficient (SCID) mice for bone regeneration. The third passage RDFCs could be seen adhered, extended and proliferated on the beta-TCP by scanning electron microscopy. The ALP activity, the calcium and phosphoniums and the osteocalcin content of dexamethasone (10(-8) M) or/and BMP-2 (100 ng ml(-1)) were significantly higher than their existence in the control group. They were the significantly highest among four groups after joint application of BMP-2 and dexamethasone. After 8 weeks of implantation, the percentage of the new bones formed area in the RDFCs+beta-TCP+BMP-2+Dex group was significantly higher than that in the RDFCs+beta-TCP+BMP-2 group. In contrast, beta-TCP, RDFCs+beta-TCP+Dex and control constructs lacked new bone formation by histological staining and histomorphometric analysis. The BMP-2+Dex could significantly promote osteogenic differentiation of RDFCs on beta-TCP. beta-TCP supported fast cellular adhesion, proliferation and differentiation of RDFCs. The feasibility of its application in periodontal tissue engineering was also proved.
Xu
Lu-Lu
LL
Department of Orthodontics, Stomatological College, Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Liu
Hong-Chen
HC
Wang
Dong-Sheng
DS
E
Ling-Ling
LL
Xu
Lin
L
Jin
Zuo-Lin
ZL
Duan
Yin-Zhong
YZ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D003795:Dental Sac / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D051379:Mice; D016513:Mice, SCID; D008855:Microscopy, Electron, Scanning / Q000379:methods; D015675:Osteocalcin / Q000737:chemistry; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D003907:Dexamethasone; D000469:Alkaline Phosphatase; D002118:Calcium