Genetic analysis for the lack of expression of the O157 antigen in an O Rough:H7 Escherichia coli strain.
The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6.
Rump
Lydia V
LV
Division of Microbiology, United States Food and Drug Administration, College Park, Maryland 20740, USA.
Feng
Peter C H
PC
Fischer
Markus
M
Monday
Steven R
SR
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019453:Escherichia coli O157 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D019081:O Antigens / Q000378:metabolism*; D009876:Operon
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D019081:O Antigens