[Screening of anti-Cry1Ac scFv from A phage display antibody library.].
OBJECTIVE
To clone human anti-Cry1Ac single-chain antibodies (scFv) from Tomlinson J phage antibody library.


METHODS
A human large phage antibody library was panned for four rounds of "adhesion-elution-amplification". The specificity of the antigen binding activity of the selected clones was identified by ELISA and the variable genes were analyzed and identified.


RESULTS
After the panning, 2 positive clones were obtained, which could bind Cry1Ac specifically. DNA sequence analysis showed that they had different antibody V region encoding genes.


CONCLUSIONS
By-passing immunization, the specific human anti-Cry1Ac scFv also can be prepared by using phage antibody library.
Wang
Yun
Y
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Liu
Yuan
Y
Wang
Xiang
X
Qian
Ya-Ping
YP
Liu
Xian-Jin
XJ
D001435:Bacteriophages / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000276:immunology
D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D057127:Single-Chain Antibodies