Design and implementation of a two-dimensional inkjet bioprinter.
Tissue engineering has the potential to improve the current methods for replacing organs and tissues and for investigating cellular process within the scope of a tissue test system. Bioprinting technology can aid in the difficult task of arranging live mammalian cells and biomaterials in viable structures for tissue engineering purposes. This paper describes a system, based on HP26 series print cartridge technology, capable of precisely depositing multiple cell types in precise patterns. The paper discusses the research, design, and implementation of the printing system, which permits control of droplet firing parameters, including firing energy, speed, and spacing. The results demonstrate the system's fine patterning ability of viable cells, including two-dimensional patterned co-cultures of two cell types. The system has been specifically designed with the flexibility to be extended to print more than two cell types and/or materials simultaneously and to layer printed patterns to form three-dimensional constructs. With these features, the printing system will serve as the foundation for a biofabrication system capable of three-dimensional cell co-cultures, i.e. tissue test systems.
Pepper
Matthew E
ME
Department of Electrical and Computer Engineering and the Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC 29634-0915, USA.
Parzel
Cheryl A
CA
Burg
Timothy
T
Boland
Thomas
T
Burg
Karen J L
KJ
Groff
Richard E
RE
D002469:Cell Separation / Q000295:instrumentation*; D016287:Computer Peripherals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007281:Ink; D044085:Microfluidics / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D023822:Tissue Engineering / Q000295:instrumentation*