title: Circulating vaspin is unrelated to insulin sensitivity in a cohort of nondiabetic humans.
abstract: OBJECTIVE
  To study the association of vaspin with glucose metabolism.
  
  
  METHODS
  Cross-sectional and intervention study.
  
  
  METHODS
  The association of serum vaspin with metabolic and anthropometric characteristics was investigated in 108 volunteers. Euglycemic-hyperinsulinemic clamps (EHC) were performed in 83 of the participants. Changes of circulating vaspin levels were additionally studied in a crossover study using 300 min EHC with lipid versus saline infusion (n=10).
  
  
  RESULTS
  Neither glucose tolerance status nor insulin sensitivity, both as measured using EHCs and using homeostasis model assessment for insulin resistance (HOMA-IR), was significantly associated with serum vaspin in the cross-sectional study. Furthermore, there was no effect of short-term lipid-induced insulin resistance due to a 300 min intravenous lipid challenge on circulating vaspin. However, circulating vaspin levels were significantly elevated in women using oral contraceptives (OC), both compared to women without OC intake (1.17+/-0.26 vs 0.52+/-0.09 ng/ml, P=0.02) and males (1.17+/-0.26 vs 0.29+/-0.04 ng/ml, P=0.01). After exclusion of OC using females and stratification according to body mass index (BMI), a significant sexual dimorphism in subjects with a BMI <25 kg/m(2) was observed (males 0.21+/-0.04 ng/ml versus females 0.70+/-0.16 ng/ml, P=0.009).
  
  
  CONCLUSIONS
  Our results support the existence of a sexual dimorphism regarding circulating vaspin. The lack of an association of serum vaspin with HOMA-IR and M value indicates, however, no major role for vaspin concerning insulin sensitivity in nondiabetic humans.
authors: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, Germany.
authors: Christian
authors: C
authors: von Loeffelholz
authors: Matthias
authors: M
authors: Möhlig
authors: Ayman M
authors: AM
authors: Arafat
authors: Frank
authors: F
authors: Isken
authors: Joachim
authors: J
authors: Spranger
authors: Knut
authors: K
authors: Mai
authors: Harpal S
authors: HS
authors: Randeva
authors: Andreas F H
authors: AF
authors: Pfeiffer
authors: Martin O
authors: MO
authors: Weickert
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001786:Blood Glucose / Q000378:metabolism*; D001794:Blood Pressure / Q000502:physiology; D015992:Body Mass Index; D016009:Chi-Square Distribution; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000502:physiology*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D018579:Patient Selection; D012044:Regression Analysis; D015843:Serpins / Q000097:blood*; D012737:Sex Factors; D055105:Waist Circumference
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; C507339:SERPINA12 protein, human; D015843:Serpins