Expression of leptin receptor gene in developing and adult zebrafish.
Interactions of leptin and leptin receptors play crucial roles during animal development and regulation of appetite and energy balance. In this study we analyzed expression pattern of a zebrafish leptin receptor gene in both developing and adult zebrafish using in situ hybridization and Q-PCR methods. Zebrafish leptin receptor message (lepr) was detected in all embryonic and larval stages examined, and in adult zebrafish. In embryonic zebrafish, lepr was mainly expressed in the notochord. As development proceeded, lepr expression in the notochord decreased, while its expression in several other tissues, including the trunk muscles and gut, became evident. In both larval and adult brains, large lepr expressing cells were detected in similar regions of the hindbrain. In adult zebrafish, lepr expression was also observed in several other brain regions including the hypothalamic lateral tuberal nucleus, the fish homolog of the arcuate nucleus. Q-PCR experiments confirmed lepr expression in the adult fish brain, and also showed lepr expression in several adult tissues including liver, muscle and gonads. Our results showed that lepr expression was both spatially and temporally regulated.
Liu
Qin
Q
Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, USA.
Chen
Yun
Y
Copeland
Donald
D
Ball
Hope
H
Duff
Robert J
RJ
Rockich
Briana
B
Londraville
Richard L
RL
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D006066:Gonads / Q000378:metabolism; D017403:In Situ Hybridization; D007814:Larva / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009672:Notochord / Q000378:metabolism; D009928:Organ Specificity; D054411:Receptors, Leptin / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*
D054411:Receptors, Leptin