[The study of cervical cancer cells model based on UV absorption spectrum].
Cell cycle is the most important process in life, and embodies all the physiological processes in the cell. In order to reflect the process of cell growth simply and accurately, the authors took cell cycle into account when the Hela cell spectral model was designed. The artificially induced cell synchronization method was employed to make Hela cell in G1, S, G2 and M phases of the cell cycle. The UV absorption spectra of these Hela cell samples were measured. The absorbability of aromatic amino acid, protein and nucleic acid in different stages of the cell cycle showed the changes in cell cycle. The subsection linear regression model of cell UV absorption spectrum was designed according to the relationship between the stage and the absorbance of samples in different phase at 204 and 260 nm. The models can be used to estimate the cell cycle after experimental verification and provide a new method for analyzing cell cycle and building cell model.
Lin
Xiao-gang
XG
Opto-Electronic Technology and Systems Key Laboratory under the Ministry of Education, Chongqing University, Chongqing 400030, China. xglin@cqu.edu.cn
Pan
Ying-jun
YJ
Guo
Yong-cai
YC
Zhang
Wei
W
Zhao
Yi-shi
YS
D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D013057:Spectrum Analysis; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms