Melatonin modulates acute testicular damage induced by carbon ions in mice.
The present study was performed to obtain evidence of the radioprotective function of melatonin at different administration levels on carbon ion-induced mouse testicular damage. Outbred Kun-Ming strain mice were divided into six groups, each composed of eight animals: control group, melatonin alone group, irradiation group and three melatonin plus irradiation-treated groups. An acute study was carried out to determine alterations in DNA-single strand break, cell apoptosis, and oxidative stress parameters as well as histopathology in mouse testis 24 h after whole-body irradiation with a single dose of 4 Gy. The results showed that pre-treatment and post-treatment with high-dose melatonin (10 mg/kg) both significantly alleviated carbon ion-induced acute testicular damage, a greater radioprotective effect being observed in the pre-treatment group. On the other hand, low-dose melatonin (1 mg/kg) had a limited radioprotective effect on irradiation-induced degeneration and DNA lesions in mouse testis. Taken together, the data suggest that prophylactic treatment with a higher dose of melatonin is probably advisable to protect against the effects of heavy-ion irradiation.
Liu
Yang
Y
Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Zhang
Hong
H
Zhang
Luwei
L
Zhang
Xu
X
Xie
Yi
Y
Zhao
Weiping
W
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002244:Carbon / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D009154:Mutation / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000517:prevention & control*; D012671:Seminiferous Tubules / Q000187:drug effects / Q000528:radiation effects; D013733:Testicular Diseases / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects
D000975:Antioxidants; D008315:Malondialdehyde; D002244:Carbon; D005978:Glutathione; D008550:Melatonin