title: Simultaneous quantification of daptomycin and rifampicin in plasma by ultra performance liquid chromatography: Application to a pharmacokinetic study.
abstract: A rapid and simple method based on ultra performance liquid chromatography (UPLC) with ultra violet detection has been developed for the determination of daptomycin (DPT) and rifampicin (RFM) in rabbit plasma using 4-nitrophenol as internal standard (IS). Sample preparation involved protein precipitation with an acetonitrile:methanol mixture and centrifugation. Chromatographic separation was achieved on an Acquity BEH C18 column (100mmx2.1mm, 1.7microm) using gradient elution with methanol and 0.1% aqueous TFA. The total analysis time was 4.5min with DPT and RFM eluting at 1.9 and 2.1min, respectively. The method was fully validated with a lower limit of quantitation (LLOQ) of 2microgmL(-1) for both DPT and RFM. The intra- and inter-day precision, measured as % relative standard deviation, were less than 12.1 for DPT and 10.7 for RFM, respectively. This validated method was successfully applied to a pharmacokinetic study involving intravenous administration of 14mgkg(-1) DPT and 30mgkg(-1) RFM to rabbits.
authors: GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, 13 Levidou Street, Kifissia 145 62, Greece.
authors: Evangelos
authors: E
authors: Gikas
authors: Fotini N
authors: FN
authors: Bazoti
authors: Panagiotis
authors: P
authors: Fanourgiakis
authors: Efstathia
authors: E
authors: Perivolioti
authors: Andreas
authors: A
authors: Roussidis
authors: Athanasios
authors: A
authors: Skoutelis
authors: Anthony
authors: A
authors: Tsarbopoulos
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000592:standards; D017576:Daptomycin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D015588:Observer Variation; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D012293:Rifampin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D017576:Daptomycin; D012293:Rifampin