title: [Twenty years development of metabolic engineering--a review].
abstract: Metabolic engineering has been developed for nearly 20 years since its beginning on 1990s, and it has significantly promoted the improvement of microbial fermentation industry. This review summarized the technology development and their applications in fermentation industry in each of the three important phases during the development of metabolic engineering. Finally, the key issues for future development and solving strategies were discussed.
authors: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. xuelizhang1981@yahoo.com
authors: Xueli
authors: X
authors: Zhang
mesh_terms: D001709:Biotechnology / Q000639:trends; D004274:DNA, Recombinant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods*; D008660:Metabolism; D015202:Protein Engineering
chemical_list: D004274:DNA, Recombinant