Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.
Osmotic stress induces changes in the expression of various genes including those associated with drought tolerance, cell wall metabolism and defense. We isolated 852 cDNA clones, the expression of which is induced by osmotic stress, from cells of a hybrid poplar (Populus alba x Populus tremula var. glandulosa) by suppression subtractive hybridization after mannitol treatment. We examined how stress affected their expression using cDNA microarray analysis, which identified 104 genes significantly up-regulated by osmotic stress. These include genes with functions related to transcription, signal transduction, cell wall metabolism and defense. Other gene transcripts encoding cysteine protease and aquaporin are also up-regulated during osmotic stress. The function of about one-third of the genes in poplar cells that were significantly up-regulated by stress is not known, suggesting that the cell suspension may offer an opportunity of finding novel genes otherwise never expressed and that we still need more information at the molecular level.
Bae
Eun-Kyung
EK
Forest Biotechnology Division, Korea Forest Research Institute, 44-3 Omokchundong, Suwon 441-350, Republic of Korea.
Lee
Hyoshin
H
Lee
Jae-Soon
JS
Noh
Eun-Woon
EW
D000222:Adaptation, Physiological / Q000235:genetics*; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D057056:Cysteine Proteases / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D017343:Genes, Plant; D008353:Mannitol; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009995:Osmosis; D032107:Populus / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic; D015854:Up-Regulation
D020346:Aquaporins; D018076:DNA, Complementary; D008353:Mannitol; D057056:Cysteine Proteases