title: Glutamine metabolism is essential for human cytomegalovirus infection.
abstract: Human fibroblasts infected with human cytomegalovirus (HCMV) were more viable than uninfected cells during glucose starvation, suggesting that an alternate carbon source was used. We have determined that infected cells require glutamine for ATP production, whereas uninfected cells do not. This suggested that during infection, glutamine is used to fill the tricarboxylic acid (TCA) cycle (anaplerosis). In agreement with this, levels of glutamine uptake and ammonia production increased in infected cells, as did the activities of glutaminase and glutamate dehydrogenase, the enzymes needed to convert glutamine to alpha-ketoglutarate to enter the TCA cycle. Infected cells starved for glutamine beginning 24 h postinfection failed to produce infectious virions. Both ATP and viral production could be rescued in glutamine-starved cells by the TCA intermediates alpha-ketoglutarate, oxaloacetate, and pyruvate, confirming that in infected cells, a program allowing glutamine to be used anaplerotically is induced. Thus, HCMV infection activates the mechanisms needed to switch the anaplerotic substrate from glucose to glutamine to accommodate the biosynthetic and energetic needs of the viral infection and to allow glucose to be used biosynthetically.
authors: Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd., School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6142, USA.
authors: Jeremy W
authors: JW
authors: Chambers
authors: Tobi G
authors: TG
authors: Maguire
authors: James C
authors: JC
authors: Alwine
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D002952:Citric Acid Cycle; D003587:Cytomegalovirus / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005947:Glucose / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007700:Kinetics; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D005973:Glutamine; D000641:Ammonia; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D005969:Glutamate Dehydrogenase; D005972:Glutaminase; D005947:Glucose