The Ni(II)-binding properties of the metallochaperone SlyD.
Metallochaperones are essential for the safe and targeted delivery of necessary yet toxic metal cofactors to their respective protein partners. In this study we examine the nickel-binding properties of the Escherichia coli protein SlyD, a factor that contributes to optimal nickel accumulation in this organism. This protein is also required for E. coli energy metabolism because it participates in the nickel insertion step during [Ni-Fe]-hydrogenase metallocenter assembly. Our study demonstrates that SlyD is a multiple nickel ion binding protein. The analysis of noncovalent metal-protein complexes via electrospray ionization mass spectrometry revealed that SlyD binds up to seven nickel ions in a noncooperative manner with submicromolar affinity (<2 microM, upper limit) and that the protein exists in a dynamic mixture of metalloforms that is dependent on the availability of nickel ions in solution. Structural analysis indicates that this metallochaperone undergoes small but distinct changes in the structure upon metal binding and that the nickel-binding sites are assembled through beta-turn formation. Although the C-terminal metal-binding domain is primarily responsible for metal chelation, we find that metal binding also perturbs the structure of the N-terminal domains. An investigation of the nickel sites by using X-ray absorption spectroscopy shows that SlyD binds nickel ions by adapting several different geometries and coordination numbers. Finally, the characterization of SlyD mutants demonstrates that the cysteine residues in the C-terminal domain confer tighter affinity as well as increased binding capacity to SlyD. On the basis of the presented data a model for nickel binding to SlyD as well as its role in nickel homeostasis is discussed.
Kaluarachchi
Harini
H
Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3H6.
Sutherland
Duncan E K
DE
Young
Alex
A
Pickering
Ingrid J
IJ
Stillman
Martin J
MJ
Zamble
Deborah B
DB
D001665:Binding Sites; D001692:Biological Transport; D002352:Carrier Proteins; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D006706:Homeostasis; D009532:Nickel / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization
D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C085606:SlyD protein, E coli; D009532:Nickel; D019696:Peptidylprolyl Isomerase