Coupling capillary electrochromatography with mass spectrometry by using a liquid-junction nano-spray interface.
Capillary electrochromatography (CEC) was coupled with mass spectrometry (MS) for the separation of some selected pesticides and drug enantiomers. CEC separations were carried out in fused silica capillaries packed with either 5microm RP(18) silica or 5microm silica modified vancomycin particles. The capillary column was connected with the MS utilizing a laboratory-made liquid-junction interface equipped with a 50microm I.D. capillary-tip positioned at a few mm from the orifice of the MS. The CEC-MS set-up was operated without external pressure assistance during the electrochromatographic run commonly used to avoid bubble formation. However a hydrostatic pressure of a few kPa was applied only to the liquid-junction interface to optimize the ion-spray due to the low I.D. of the capillary-tip. In order to optimize the CEC-MS method, several experimental parameters were studied, namely the inlet pressure, the hydrostatic pressure into the liquid-junction interface, the type of sheath-liquid and the mobile phase. The application of an inlet pressure influenced only analyte retention times that were shortened by increasing the pressure. On the contrary the hydrostatic pressure applied to the interface increased the flow rate into the tip also increasing the ion-signal recorded in the mass spectrometry. The ion-signal raised almost linearly by increasing the outlet pressure till 3.5kPa and then decreased. The separation of the selected pesticides was not influenced at all changing the hydrostatic pressure on the interface. Some basic enantiomeric compounds of pharmaceutical interest were successfully separated by CEC achieving good resolution. They were detected by MS with limit of detection in a range of 0.24-0.60microg/mL.
D'Orazio
Giovanni
G
Institute of Chemical Methodologies, National Research Council, Area della Ricerca di Roma I, Via Salaria Km 29.300, 00015 Monterotondo Scalo (Rome), Italy.
Fanali
Salvatore
S
D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations