title: Interaction between oligomers of stefin B and amyloid-beta in vitro and in cells.
abstract: To contribute to the question of the putative role of cystatins in Alzheimer disease and in neuroprotection in general, we studied the interaction between human stefin B (cystatin B) and amyloid-beta-(1-40) peptide (Abeta). Using surface plasmon resonance and electrospray mass spectrometry we were able to show a direct interaction between the two proteins. As an interesting new fact, we show that stefin B binding to Abeta is oligomer specific. The dimers and tetramers of stefin B, which bind Abeta, are domain-swapped as judged from structural studies. Consistent with the binding results, the same oligomers of stefin B inhibit Abeta fibril formation. When expressed in cultured cells, stefin B co-localizes with Abeta intracellular inclusions. It also co-immunoprecipitates with the APP fragment containing the Abeta epitope. Thus, stefin B is another APP/Abeta-binding protein in vitro and likely in cells.
authors: Department of Biochemistry, Molecular and Structural Biology, JoZef Stefan Institute, Jamova 39, Slovenia.
authors: Katja
authors: K
authors: Skerget
authors: Ajda
authors: A
authors: Taler-Vercic
authors: Andrej
authors: A
authors: Bavdek
authors: Vesna
authors: V
authors: Hodnik
authors: Slavko
authors: S
authors: Ceru
authors: Magda
authors: M
authors: Tusek-Znidaric
authors: Tiina
authors: T
authors: Kumm
authors: Didier
authors: D
authors: Pitsi
authors: Marusa
authors: M
authors: Pompe-Novak
authors: Peep
authors: P
authors: Palumaa
authors: Salvador
authors: S
authors: Soriano
authors: Natasa
authors: N
authors: Kopitar-Jerala
authors: Vito
authors: V
authors: Turk
authors: Gregor
authors: G
authors: Anderluh
authors: Eva
authors: E
authors: Zerovnik
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D052160:Benzothiazoles; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D055313:Cystatin B / Q000737:chemistry*; D019281:Dimerization; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization; D020349:Surface Plasmon Resonance; D013844:Thiazoles / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D000939:Epitopes; D013844:Thiazoles; C009462:thioflavin T; D055313:Cystatin B