Compound K protects MIN6N8 pancreatic beta-cells against palmitate-induced apoptosis through modulating SAPK/JNK activation.
Chronic elevation of NEFAs (non-esterified fatty acids) due to insulin resistance and obesity has been shown to be associated with increased beta-cell apoptosis and with the aetiology of the reduced beta-cell mass of Type 2 diabetes. SAPK (stress-activated protein kinase)/JNK (c-Jun N-terminal kinase) have been implicated in the control of apoptosis. C-K [compound K; 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol] is the main intestinal bacterial metabolite of protopanaxadiol ginsenosides. Currently, little is known about the effects of C-K on beta-cells with the presence of NEFAs. The aim of the present study was to investigate the in vitro protective effect of C-K on MIN6N8 mouse insulinoma beta-cells against NEFA-induced apoptosis, as well as the modulating effect on SAPK/JNK activation. Our results have shown that C-K inhibited the palmitate-induced apoptosis through modulating SAPK/JNK activation. We conclude that C-K protects against beta-cell death and that, by anti-apoptotic activity, C-K may contribute to the previously reported anti-diabetic actions of ginseng.
Kim
Kyong
K
Functional Food Technology Research Group, Research Division for Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Songnam-si, Kyonggi-do 463-746, Republic of Korea.
Kim
Dong-Hyun
DH
Kim
Hye Young
HY
D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005230:Fatty Acids, Nonesterified / Q000633:toxicity; D036145:Ginsenosides / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D010168:Palmitates / Q000494:pharmacology*
C540687:20-O-beta-D-glucopyranosyl-protopanaxadiol; D050034:Antidiuretic Agents; D005230:Fatty Acids, Nonesterified; D036145:Ginsenosides; D010168:Palmitates; D048031:JNK Mitogen-Activated Protein Kinases