Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes.
When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.
Arnett
Clint M
CM
U.S. Army Engineering Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61826, USA. clint.arnett@usace.army.mil
Marsh
Charles P
CP
Welch
Charles R
CR
Strano
Michael S
MS
Han
Jae-Hee
JH
Gray
Jeffry H
JH
Carlson
Thomas A
TA
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman; D013696:Temperature
D037742:Nanotubes, Carbon; D004247:DNA; D011088:DNA Ligases