Identification of ricinoleic acid as an inhibitor of Ca2+ signal-mediated cell-cycle regulation in budding yeast.
Free fatty acids exhibit diverse biological effects such as the regulation of immune responses in humans and animals. To investigate the biological effect of fatty acids in the model eukaryotic organism yeast, we examined the activity of various fatty acids in a yeast-based drug-screening system designed to detect the small-molecule compounds that inhibit Ca(2+)-signal-mediated cell-cycle regulation. Among the fatty acids examined, ricinoleic acid markedly alleviated the deleterious physiological effects induced by the compelled activation of Ca(2+) signaling by external CaCl(2), such as the polarized bud growth and the growth arrest in the G(2) phase. In accordance with the physiological consequences induced by ricinoleic acid, it diminished the Ca(2+)-induced phosphorylation of Cdc28p at Tyr-19, concomitant with the decrease in the Ca(2+)-stimulated expression levels of Cln2p and Swe1p.
Attrapadung
Siriluck
S
Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Yoshida
Jun
J
Kimura
Ken-ichi
K
Mizunuma
Masaki
M
Miyakawa
Tokichi
T
Thanomsub
Benjamas Wongsatayanon
BW
D042846:CDC2-CDC28 Kinases / Q000378:metabolism; D002122:Calcium Chloride / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000494:pharmacology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D012277:Ricinoleic Acids / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000378:metabolism
C082721:CLN2 protein, S cerevisiae; D018797:Cell Cycle Proteins; D016213:Cyclins; D006131:Growth Inhibitors; D012277:Ricinoleic Acids; D029701:Saccharomyces cerevisiae Proteins; C099685:SWE1 protein, S cerevisiae; D011505:Protein-Tyrosine Kinases; D042846:CDC2-CDC28 Kinases; C030521:ricinoleic acid; D002122:Calcium Chloride