Simultaneous determination of five tetracycline and macrolide antibiotics in feeds using HPCE.
This work demonstrates the potential of HPCE in the analysis of antibiotics in a complex matrix such as feedstuffs. Using 20 mM citric acid-40 mM Na(2)HPO(4) buffer (pH 2.65), the five antibiotics, tetracycline, oxytetracycline, doxycycline, tilmicosin, and tylosin were successfully separated at 30 kV in a 64.5 cm x 75 microm id capillary. Good repeatability, stability, and reliability of the method were supported by <10% CV with mean recoveries of >70%, and the limit of detection of the five analytes was 0.5-1 mg/kg. It was for the first time that a capillary electrophoretic method was employed to simultaneously detect five tetracycline and macrolide antibiotics in animal feeds.
Tong
Jing
J
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Rao
Qinxiong
Q
Zhu
Kui
K
Jiang
Zhigang
Z
Ding
Shuangyang
S
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000592:standards; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D018942:Macrolides / Q000032:analysis; D012015:Reference Standards; D013754:Tetracyclines / Q000032:analysis
D000900:Anti-Bacterial Agents; D018942:Macrolides; D013754:Tetracyclines