title: Efficacy of several vaccination programmes in commercial layer and broiler breeder hens against experimental challenge with Salmonella enterica serovar Enteritidis.
abstract: Two experiments were performed to evaluate the protective effect of various vaccination combinations given at 5 and 9 weeks of age against experimental challenge with Salmonella enterica serovar Enteritidis (SE) phage type 4 at 12 weeks of age. In Experiment 1, groups of commercial layers were vaccinated by one of the following programmes: Group 1, two doses of a SE bacterin (Layermune SE); Group 2, one dose of a live Salmonella enterica serovar Gallinarum vaccine (Cevac SG9R) followed by one dose of the SE bacterin; Group 3, one dose of each of two different multivalent inactivated vaccines containing SE cells (Corymune 4K and Corymune 7K; and Group 4, unvaccinated, challenged controls. In Experiment 2, groups of broiler breeders were vaccinated by the same programmes as Groups 1 and 2 above while Group 3 was an unvaccinated, challenged control group. All vaccination programmes and the challenge induced significant (P < 0.05) seroconversion as measured by enzyme-linked immunosorbent assay. Overall, in both experiments, all vaccination schemes were significantly effective in reducing organ (spleen, liver and caeca) colonization by the challenge strain as well as reducing faecal excretion for at least 3 weeks. Vaccinated layers in Groups 1 and 2 and broiler breeders in Group 2 showed the greatest reduction in organ colonization and the least faecal excretion. In Experiment 1, layers vaccinated with multivalent inactivated vaccines containing a SE component (Group 3) were only moderately protected, indicating that such a vaccination programme may be useful in farms with good husbandry and housing conditions and low environmental infectious pressure by Salmonella.
authors: Avian Pathology Group, FCAV-Unesp, Jaboticabal-SP, Brazil.
authors: Rafael Antonio Casarin
authors: RA
authors: Penha Filho
authors: Jacqueline Boldrin
authors: JB
authors: de Paiva
authors: Yuli Melisa Sierra
authors: YM
authors: Arguello
authors: Mariana Dias
authors: MD
authors: da Silva
authors: Yannick
authors: Y
authors: Gardin
authors: Fernando
authors: F
authors: Resende
authors: Angelo Berchieri
authors: AB
authors: Berchieri Junior
authors: Luiz
authors: L
authors: Sesti
mesh_terms: D000818:Animals; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005260:Female; D008099:Liver / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022562:Salmonella Vaccines; D012477:Salmonella enteritidis / Q000276:immunology*; D013154:Spleen / Q000382:microbiology; D014611:Vaccination / Q000662:veterinary*; D017778:Vaccines, Combined
chemical_list: D022562:Salmonella Vaccines; D017778:Vaccines, Combined