Biological effect of anaphylatoxin C5a on the generation of anti-inflammatory substances in leukocyte adsorption.
Anaphylatoxins, which are involved in both pro-inflammatory processes and a variety of anti-inflammatory effects, are produced during granulocyte and monocyte adsorptive apheresis. We noticed the anti-inflammatory effects of C5a, the strongest anaphylatoxin, in granulocyte and monocyte adsorptive apheresis. The aim of this study was to investigate the effect of C5a on interleukin-1 receptor antagonist (IL-1ra) and hepatocyte growth factor (HGF) generation in granulocyte and monocyte adsorption. Peripheral blood containing nafamostat mesilate as an endogenous complement activation inhibitor was divided into four groups: (1) no recombinant C5a added, no contact with cellulose acetate (CA) beads (control group); (2) no C5a added, contact with CA beads; (3) C5a added, no contact with CA beads; and (4) C5a added, contact with CA beads. After incubation, IL-1ra and HGF in plasma were measured. IL-1ra was significantly higher in group 3, in which only C5a was added in the absence of CA beads, compared to groups 2 (P < 0.01) and 4 (P < 0.05). HGF was significantly higher only in group 4, in which C5a was added in the presence of CA beads (P < 0.05), but did not increase in the absence of CA beads. C5a can directly induce IL-1ra generation without the granulocyte and monocyte adsorption stimuli to CA beads, but can synergistically induce HGF generation with the adsorption stimuli, indicating C5a has different effects on IL-1ra and HGF generation.
Nishise
Shoichi
S
Department of Gastroenterology, Yamagata University School of Medicine, Yamagata, Japan. nishise-sic@umin.ac.jp
Takeda
Yuji
Y
Nishise
Yuko
Y
Fujishima
Shoichiro
S
Orii
Tomohiko
T
Otake
Sayaka
S
Sato
Takeshi
T
Sasaki
Yu
Y
Takeda
Hiroaki
H
Kawata
Sumio
S
D000327:Adsorption; D001550:Benzamidines; D001781:Blood Component Removal / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D015936:Complement C5a / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism
D001550:Benzamidines; D006146:Guanidines; D053590:Interleukin 1 Receptor Antagonist Protein; C005062:acetylcellulose; D017228:Hepatocyte Growth Factor; D015936:Complement C5a; D002482:Cellulose; C032855:nafamostat