The Influence of Alpha-fetoprotein on Natural Suppressor Cell Activity and Ehrlich Carcinoma Growth.
The influence of alpha-fetoprotein (AFP) on the bone marrow (BM) natural suppressor (NS) cells of intact Ehrlich carcinoma -bearing CBA mice was studied. Bone marrow NS cells were fractionated into three fractions by isopycnic centrifugation on percoll gradients: NS1 (rho=1.080 g/ml), NS2 (rho=1.090 g/ml) and NS3 (1.100>rho>1.090 g/ml). These fractions were highly different in their sensitivity to known NS cell inductors (interleukin (IL)-2, IL-3 or histamine). None of the NS fractions isolated from the intact mice spontaneously produced antiproliferative activity, however, they showed a high level of NS (antiproliferative and natural killer cell inhibitory) activity under the influence of AFP. A single injection of AFP to intact mice led to an increase of spontaneous NS activity and the inhibition of natural killer cell activity. NS activity, especially NS2, was increased in when tumor cells were subcutaneously inoculated three days after AFP injection. In the AFP-treated mice, the tumor mass at 14 days was 60% larger than that in the untreated mice. Our data confirmed that AFP is a tumor marker that can inhibit cancer immunity and plays a role in cancer pathogenesis.
Belyaev
Nikolai Nikolaevich
NN
Institute of Molecular Biology and Biochemistry, Kazakhstan Medical University, Almaty 050022, Kazakhstan.
Bogdanov
Andrei-Yurievich
AY
Savvulidi
Philipp-Georgievich
PG
Krasnoshtanov
Vladimir-Konstantinovich
VK
Tleulieva
Raikhan-Tleulievna
RT
Alipov
Gabit-Kaimovich
GK
Sekine
Ichiro
I
Bae
Jun-Sang
JS
Lee
Jeong-Beom
JB
Min
Young-Ki
YK
Yang
Hun-Mo
HM