Analysis of internal osmolality in developing coral larvae, Fungia scutaria.
Coral species throughout the world are facing severe local and global environmental pressures. Because of the pressing conservation need, we are studying the reproduction, physiology, and cryobiology of coral larvae with the future goal of cryopreserving and maintaining these organisms in a genome resource bank. Effective cryopreservation involves several steps, including the loading and unloading of cells with cryoprotectant and the avoidance of osmotic shock. In this study, during the time course of coral larvae development of the mushroom coral Fungia scutaria, we examined several physiologic factors, including internal osmolality, percent osmotically active water, formation of mucus cells, and intracellular organic osmolytes. The osmotically inactive components of the cell, V(b), declined 33% during development from the oocyte to day 5. In contrast, measurements of the internal osmolality of coral larvae indicated that the internal osmolality was increasing from day 1 to day 5, probably as a result of the development of mucus cells that bind ions. Because of this, we conclude that coral larvae are osmoconformers with an internal osmolality of about 1,000 mOsm. Glycine betaine, comprising more than 90% of the organic osmolytes, was found to be the major organic osmolyte in the larvae. Glycerol was found in only small quantities in larvae that had been infected with zooxanthellae, suggesting that this solute did not play a significant role in the osmotic balance of this larval coral. We were interested in changes in cellular characteristics and osmolytes that might suggest solutes to test as cryoprotectants in order to assist in the successful cryopreservation of the larvae. More importantly, these data begin to reveal the basic physiological events that underlie the move from autonomous living to symbiosis.
Hagedorn
Mary
M
Department of Reproductive Sciences, Smithsonian National Zoological Park, Washington, DC 20008, USA. hagedornm@si.edu
Carter
Virginia L
VL
Ly
Steven
S
Andrell
Raymond M
RM
Yancey
Paul H
PH
Leong
Jo-Ann C
JA
Kleinhans
Frederick W
FW
D000818:Animals; D037421:Anthozoa / Q000737:chemistry* / Q000254:growth & development; D001622:Betaine / Q000032:analysis*; D003247:Conservation of Natural Resources / Q000379:methods; D005260:Female; D005990:Glycerol / Q000032:analysis*; D006254:Hawaii; D006651:Histocytochemistry; D007814:Larva / Q000502:physiology; D009994:Osmolar Concentration
D001622:Betaine; D005990:Glycerol