Expression of new red cell-related genes in erythroid differentiation.
Using a suppression subtractive hybridization method, we have previously identified genes differentially expressed in erythroid cells heterozygous for large deletions in beta-like globin cluster. Herein, we investigated the expression of four newly detected red cell-related genes in erythroid differentiation. ARID1B and TSPYL1, genes with chromatin remodeling properties, presented similar patterns of expression with an upregulation after erythropoietin (EPO) addition, similar to previous data found in reticulocytes. ZHX2, a transcriptional repressor, was downregulated, and a redoxin-related gene, SH3BGRL2, had higher levels of expression on differentiation. These are the first investigations of these newly described genes in erythroid differentiation and demonstrate that the expression of these genes is affected by EPO stimulation. These genes may participate in globin regulation and may be important in the normal physiology of erythrocytes.
De Andrade
Tiago
T
Federal University of Alagoas, Campus Arapiraca, Arapiraca, AL, Brazil. deandrade.tiago@gmail.com
Moreira
Luciana
L
Duarte
Adriana
A
Lanaro
Carolina
C
De Albuquerque
Dulcinéia
D
Saad
Sara
S
Costa
Fernando
F
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D009000:Monocytes / Q000166:cytology; D009687:Nuclear Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D014157:Transcription Factors / Q000235:genetics; D055544:beta-Globins / Q000235:genetics*
C497837:ARID1B protein, human; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C510183:TSPYL1 protein, human; D014157:Transcription Factors; D055544:beta-Globins