MHC haplotype and susceptibility to experimental infections (Salmonella Enteritidis, Pasteurella multocida or Ascaridia galli) in a commercial and an indigenous chicken breed.
In three independent experimental infection studies, the susceptibility and course of infection of three pathogens considered of importance in most poultry production systems, Ascaridia galli, Salmonella Enteritidis and Pasteurella multocida were compared in two chicken breeds, the indigenous Vietnamese Ri and the commercial Luong Phuong. Furthermore, the association of the Major Histocompatibility Complex (MHC) with disease-related parameters was evaluated, using alleles of the LEI0258 microsatellite as markers for MHC haplotypes. The Ri chickens were found to be more resistant to A. galli and S. Enteritidis than commercial Luong Phuong chickens. In contrast, the Ri chickens were more susceptible to P. multocida, although production parameters were more affected in the Luong Phuong chickens. Furthermore, it was shown that the individual variations observed in response to the infections were influenced by the MHC. Using marker alleles of the microsatellite LEI0258, which is located within the MHC region, several MHC haplotypes were identified as being associated with infection intensity of A. galli. An association of the MHC with the specific antibody response to S. Enteritidis was also found where four MHC haplotypes were shown to be associated with high specific antibody response. Finally, one MHC haplotype was identified as being associated with pathological lesions and mortality in the P. multocida experiment. Although not statistically significant, our analysis suggested that this haplotype might be associated with resistance. These results demonstrate the presence of local genetic resources in Vietnamese chickens, which could be utilized in breeding programmes aiming at improving disease resistance.
Schou
T W
TW
Department of Veterinary Pathobiology, The Faculty of Life Sciences, University of Copenhagen, Stigbojlen 4, DK-1870 Frederiksberg C, Denmark; Department of Human Health and Safety, The DHI Group, Kogle Allé 2, 2970 Horsholm, Denmark. Electronic address: tws@dhigroup.com.
Labouriau
R
R
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark.
Permin
A
A
Department of Veterinary Pathobiology, The Faculty of Life Sciences, University of Copenhagen, Stigbojlen 4, DK-1870 Frederiksberg C, Denmark; Department of Human Health and Safety, The DHI Group, Kogle Allé 2, 2970 Horsholm, Denmark.
Christensen
J P
JP
Department of Veterinary Pathobiology, The Faculty of Life Sciences, University of Copenhagen, Stigbojlen 4, DK-1870 Frederiksberg C, Denmark.
Sørensen
P
P
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark.
Cu
H P
HP
Department of Bacteriology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Viet Nam.
Nguyen
V K
VK
Department of Parasitology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Viet Nam.
Juul-Madsen
H R
HR
Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark.
D000483:Alleles; D000818:Animals; D001197:Ascaridia / Q000276:immunology; D001198:Ascaridiasis / Q000276:immunology / Q000662:veterinary*; D002645:Chickens / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D004247:DNA / Q000235:genetics; D004198:Disease Susceptibility / Q000276:immunology / Q000662:veterinary; D005838:Genotype; D006239:Haplotypes / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D010270:Parasite Egg Count / Q000662:veterinary; D010326:Pasteurella Infections / Q000276:immunology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology
D004247:DNA