Persicarin from water dropwort (Oenanthe javanica) protects primary cultured rat cortical cells from glutamate-induced neurotoxicity.
The n-BuOH fraction of O. javanica significantly protected the primary cultures of rat cortical cells exposed to glutamate. Four flavonoids yielded from this fraction through bioactivity-guidance. The isolated compounds, identified as isorhamnetin (1), afzelin (2), hyperoside (3) and persicarin (4), were evaluated in vitro for their neuroprotective activity. Persicarin (4), the main constituent of O. javanica, showed significant neuroprotective activities in glutamate-injured rat cortical cells. Persicarin diminished calcium influx and inhibited the subsequent overproduction of nitric oxide and intracellular peroxide. In addition, persicarin significantly restored the reduced activities of glutathione (GSH) reductase and glutathione peroxidase, and the contents of GSH induced by glutamate. These results support a conclusion that persicarin greatly contributes to the neuroprotective activities of O. javanica.
Ma
Choong Je
CJ
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Lee
Ki Yong
KY
Jeong
Eun Ju
EJ
Kim
Seung Hyun
SH
Park
Junghyun
J
Choi
Yun Hee
YH
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid / Q000009:adverse effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis; D031050:Oenanthe / Q000737:chemistry*; D010545:Peroxides / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D047309:Flavones; D005419:Flavonoids; D018696:Neuroprotective Agents; D010545:Peroxides; D010936:Plant Extracts; C551071:persicarin; D009569:Nitric Oxide; D018698:Glutamic Acid; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D002118:Calcium