title: Effects of extracellular matrix on differentiation of human bone marrow-derived mesenchymal stem cells into smooth muscle cell lineage: utility for cardiovascular tissue engineering.
abstract: BACKGROUND
  Bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into various types of cell, and the extracellular matrix (ECM) is acknowledged to be important for the regulation of cell functions. In this study, we demonstrated the effects of ECMs on the differentiation of human bone marrow-derived MSCs into a smooth muscle cell (SMC) lineage.
  
  
  METHODS
  Human MSCs (hMSCs) were cultured on dishes coated with 3 types of ECM including laminin (LM), collagen type IV (Col-IV) and fibronectin for 7 days, and simultaneously cultured on a noncoated dish as a control. Cell numbers of these cultured hMSCs were counted, and their expression of SMC-specific genes and proteins was evaluated. hMSCs were then seeded on LM-coated biodegradable sheets and implanted into rat subcutaneous space. After 2 weeks of implantation, these tissues were evaluated.
  
  
  RESULTS
  The number of hMSCs was significantly increased by culture on Col-IV-coated dishes. The expression of SMC-specific genes and proteins (alpha-smooth muscle actin, ASMA; h1-calponin, CALP) in hMSC was significantly upregulated from culture on LM-coated dishes. LM-coated sheets showed a significantly increased expression of ASMA and CALP protein in vivo. Moreover, a fully differentiated marker (SM2) was expressed in the in vivo implanted hMSCs in the course of 2 weeks on the LM-coated sheet.
  
  
  CONCLUSIONS
  These results suggest that the signal transduction of the cell-matrix interaction for the differentiation of hMSCs into SMCs was activated when cultured with LM. LM-coated materials may thus be useful for cardiovascular tissue engineering.
authors: Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan.
authors: Shuichi
authors: S
authors: Suzuki
authors: Yuji
authors: Y
authors: Narita
authors: Aika
authors: A
authors: Yamawaki
authors: Yosuke
authors: Y
authors: Murase
authors: Makoto
authors: M
authors: Satake
authors: Masato
authors: M
authors: Mutsuga
authors: Hideki
authors: H
authors: Okamoto
authors: Hideaki
authors: H
authors: Kagami
authors: Minoru
authors: M
authors: Ueda
authors: Yuichi
authors: Y
authors: Ueda
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002319:Cardiovascular System / Q000166:cytology; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D023822:Tissue Engineering
chemical_list: D007797:Laminin