[Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro].
We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro. With 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay), we used two phycobiliproteins, R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) prepared from Porphyra yezoensis, to determine the killing rates and apoptosis rates of human liver cancer cells (SMMC-7721) mediated by laser. When the concentration of R-PE was 120 mg/L, the survival rate of human liver cancer cells was 27% after treated by Argon laser with 100 J/cm2 doses, while the survival rate in the control group (without adding R-PE) was 65%. When the C-PC concentration was 120 mg/L, the survival cell rate was 47% after treated by He-Ne laser with 35 J/cm2 dose, while the survival rate in the control group (without adding C-PC) was 70%. After handled only with these two kinds of phycobiliproteins for 72 h, the growth of cancer cells presented significant inhibition. The maximal inhibition rates reached up to 31% with R-PE (120 mg/L concentration) and 27% with C-PC (250 mg/L concentration) respectively. After irradiated by laser for 8 h, the maximal cell apoptosis rates were 31.54% with R-PE and 32.54% with C-PC, respectively. It indicated that R-PE and C-PC extracted from Porphyra yezoensis could develop to new photosensitizers for cancer photodynamic therapy.
Wang
Yuan
Y
Shanghai Ocean University, Shanghai 201306, China.
Cai
Chuner
C
Li
Bailin
B
Liu
Chengchu
C
He
Peimin
P
D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D006801:Humans; D007834:Lasers; D008113:Liver Neoplasms / Q000473:pathology*; D055668:Photochemical Processes; D010778:Photochemotherapy / Q000379:methods; D052979:Phycobiliproteins / Q000302:isolation & purification / Q000494:pharmacology*; D010799:Phycoerythrin / Q000302:isolation & purification / Q000494:pharmacology; D044664:Porphyra / Q000737:chemistry
D052979:Phycobiliproteins; D010799:Phycoerythrin