[Channel radiofrequency volumetric reduction of tongue base a vitro porcine three-dimensional reconstruction experiment for the treatment of obstructive sleep apnea hypopnea syndrome].
OBJECTIVE
To investigate lesion size caused by channel radiofrequency volumetric reduction of porcine tongue base in vitro using the technique of three-dimensional reconstruction. And to evaluate safety about channel radiofrequency volumetric reduction of tongue base.


METHODS
Eighteen fresh porcine tongues were randomly separated into six groups,and each group had three ones. The tongue bases were designed six points according to description of Powell. Tongues base were acted on 10 s and 6 level by Coblation radiofrequency system and were cut into serial freezing histological sections. These segments were sectioned at 20 microm on the injury lesion and stained with H & E. Collected 2D digital imagine of order histological sections, drawn and cut apart part of the lesion of these sections. Images were processed IPS and were taken three-dimensional reconstruction and statistics analyzes with SPSS10.0.


RESULTS
The mean value of tongue base lesion volumes among points was (359.5 +/- 5.6) mm3, (364.3 +/- 7.0) mm, (363.7 +/- 7.2) mm3, (354.1 +/- 11.8) mm3, (349.4 +/- 17.2) mm3, (353.5 +/- 7.9) mm3 separately. Statistic analysis by one-way ANOVA showed that there was a insignificant difference between the groups (P>0.05).


CONCLUSIONS
These results demonstrated no significant effect lesion size in channel radiofrequency volumetric reduction in the different points of the tongue base. These data also indicated that coblation radiofrequency system is a safe method for obstructive sleep apnea hypopnea syndrome.
Guan
Jian
J
Department of Otolaryngology, and Sleep Disorders Center, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, China.
Yi
Hongliang
H
Yu
Dongzhen
D
Huang
Yanyan
Y
Yin
Shankai
S
D000818:Animals; D017115:Catheter Ablation / Q000009:adverse effects / Q000379:methods*; D066298:In Vitro Techniques; D020181:Sleep Apnea, Obstructive / Q000601:surgery; D025321:Surgery, Computer-Assisted; D013552:Swine; D014059:Tongue / Q000293:injuries* / Q000601:surgery*; D016896:Treatment Outcome