Eutigoside C attenuates radiation-induced crypt injury in the mouse intestine.
On Jeju Island, South Korea, the leaves of Eurya emarginata have been traditionally used to treat ulcers or as a diuretic. Eutigoside C isolated from the leaves has been reported to have in vitro anti-inflammatory effects. We evaluated the radioprotective effects of eutigoside C on jejunal cell apoptosis and crypt survival in mice subjected to gamma irradiation. In addition, the ability of eutigoside C to protect against radiation-induced oxidative stress was examined by evaluating the activities of superoxide dismutase (SOD) and catalase (CAT) in radiation-induced hepatic injury. Eutigoside C was administered intraperitoneally at 48, 12, and 1 h before irradiation. The administration of eutigoside C (10, 50, or 100 mg/kg body weight) before irradiation protected the intestinal crypts from radiation-induced apoptosis (p < 0.05), and attenuated radiation-induced decrease of villous height (p < 0.05). Pretreating mice prior to irradiation with eutigoside C (100 mg/kg) significantly improved the survival of the jejunal crypt (p < 0.01). The dose reduction factor was 1.09 at 3.5 days after irradiation. Treatment of eutigoside C prior to irradiation significantly protected SOD and CAT activities in radiation-induced hepatic injury (p < 0.05). These results suggest that eutigoside C is a useful radioprotector capable of defending intestinal progenitor cells against indirect depletion, such as oxidative stress and inflammatory response caused by gamma irradiation.
Moon
Changjong
C
Department of Veterinary Anatomy, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju, South Korea.
Ahn
Kyuyoung
K
Kim
Jihoon
J
Kim
Jeongtae
J
Kim
Sung-Ho
SH
Oh
Tae Heon
TH
Lee
Nam Ho
NH
Jee
Youngheun
Y
Hyun
Jin Won
JW
Park
Jae Woo
JW
Shin
Taekyun
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D005720:Gamma Rays; D005960:Glucosides / Q000494:pharmacology*; D007422:Intestines / Q000528:radiation effects*; D007583:Jejunum / Q000528:radiation effects; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D008517:Phytotherapy; D011833:Radiation Injuries, Experimental / Q000188:drug therapy*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D005960:Glucosides; D010636:Phenols; D011837:Radiation-Protective Agents; C505499:eutigoside C; D002374:Catalase; D013482:Superoxide Dismutase