[Reorganization of microtubules as a response to realization of NO (II) signal pathways in plant cell].
Effects of exogenic NO donor, sodium nitroprusside, on orientation and organization of cortical microtubules in Arabidopsis thaliana root cells that express GFP-MAP4 were studied in vivo. It was found that sodium nitroprusside treatment (10-500 microM, 24 h) caused the acceleration of primary root growth and enhanced initiation of root hairs in differentiation zone. The influence of sodium nitroprusside revealed in alterations of cortical: microtubules orientation and organization in different types of cells of A. thaliana root. The most sensitive to sodium nitroprusside exposure were microtubules in epidermal cells of elongation zone where native transverse orientation of cortical microtubules turned into random, oblique or longitudinal relatively to primary root axis. We suppose that NO as one of the intracellular secondary messengers triggers cells differentiation by cortical microtubules reorientation possibly via tubulin nitrotyrosination.
Emets
A I
AI
Krasilenko
Iu A
IuA
Sheremet
Ia A
IaA
Blium
Ia B
IaB
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D020030:Nitric Oxide Donors; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins; D009569:Nitric Oxide