Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.
Cangelosi
Gerard A
GA
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
Weigel
Kris M
KM
Lefthand-Begay
Clarita
C
Meschke
John S
JS
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005798:Genes, Bacterial; D050296:Microbial Viability / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014871:Water Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012322:RNA Precursors; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S