Isolation of Bothrops jararaca snake antithrombin from the supernatant of fibrinogen purification.
A novel method of antithrombin (AT) purification from Bothrops jararaca snake plasma was developed to obtain this protein using a waste supernatant from B. jararaca fibrinogen purification. The AT purification was achieved by affinity chromatography on HiTrap Heparin HP. The results showed an efficient purification process yielding pure AT (purity 65-fold and specific activity 368.91). In conclusion, we showed a feasible purification method of AT from B. jararaca plasma using a discarded material. This feature is important, considering the limitation of material, such as snake plasma, and could also be useful to obtain pure plasma proteins from other animals, including human plasma.
Morais-Zani
K
K
Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil.
Tanaka
A S
AS
Tanaka-Azevedo
A M
AM
D000327:Adsorption; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D000991:Antithrombins / Q000737:chemistry / Q000302:isolation & purification*; D017609:Barium Compounds / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D015415:Biomarkers / Q000378:metabolism; D001777:Blood Coagulation; D017837:Bothrops / Q000378:metabolism*; D002417:Cattle; D002712:Chlorides / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry / Q000302:isolation & purification*; D013917:Thrombin / Q000737:chemistry
D000991:Antithrombins; D017609:Barium Compounds; D015415:Biomarkers; D002712:Chlorides; C024986:barium chloride; D005340:Fibrinogen; D013917:Thrombin; D000645:Ammonium Sulfate