title: Inhibitory effects of 1,3-thiazine derivatives on melanogenesis.
abstract: OBJECTIVE
  The aim of this study was to identify a novel skin-depigmenting agent from synthetic 1,3-thiazine derivatives.
  
  
  METHODS
  We investigated the inhibitory effects of six kinds of 1,3-thiazine derivative on melanogenesis by examining their effects on tyrosinase activity and melanin biosynthesis in melan-a cells and the zebrafish model.
  
  
  RESULTS
  Of the six compounds, 4-hydroxy-2,6-dimethyl-5,6-dihydro-4H-1,3-thiazine (TZ-6) had the strongest anti-melanogenic effects in cultured melan-a cells (30.4% inhibition at 100 microm). In addition, TZ-6 exhibited an inhibitory effect on mushroom and cellular tyrosinase. Based on the results of Western blotting, TZ-6 reduced the expression of tyrosinase at 100 microm. Additionally, TZ-6 reduced body pigmentation and inhibited tyrosinase activity in the zebrafish model.
  
  
  CONCLUSIONS
  The results have provided useful information for the development of a skin whitening agent.
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Gyeonggi-do, Korea.
authors: Sang Keun
authors: SK
authors: Ha
authors: Mamoru
authors: M
authors: Koketsu
authors: Minjae
authors: M
authors: Lee
authors: Eunjung
authors: E
authors: Moon
authors: Sung-Hoon
authors: SH
authors: Kim
authors: Tae-Jin
authors: TJ
authors: Yoon
authors: Sun Yeou
authors: SY
authors: Kim
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012880:Skin Pigmentation / Q000187:drug effects*; D013843:Thiazines / Q000302:isolation & purification / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: C549227:4-hydroxy-2,6-dimethyl-5,6-dihydro-4H-1,3-thiazine; D004791:Enzyme Inhibitors; D008543:Melanins; D013843:Thiazines; D014442:Monophenol Monooxygenase