The generation of plasmacytoid and conventional dendritic cells with M-CSF.
Mice lacking the ligand for Flt-3 (CD135) have a massive deficit of dendritic cells (DC) in all organs. This phenotype of FL (FL) knockout mice suggested that FL was the archetypal DC poietin in the steady state. However, FL knockout mice also have reduced numbers of common lymphoid progenitors (CLP) and common myeloid progenitors (CMP) so it is possible that FL deficiency may limit the ability of other growth factors to drive DC development by limiting the pool of progenitor cells available. We found that DC development could be driven from BM cells of FL knockout mice using the myeloid growth factor M-CSF. The M-CSF-driven DC (MDC) developed independently of FL and resembled the DC types present in the spleen in the steady state.
O'Keeffe
Meredith
M
Research Department, Bavarian Nordic GmbH, Martinsried, Germany.
Fancke
Ben
B
Hochrein
Hubertus
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D039521:CD11c Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D051379:Mice; D018345:Mice, Knockout; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics
D039521:CD11c Antigen; D016173:Macrophage Colony-Stimulating Factor; D051941:fms-Like Tyrosine Kinase 3; D017493:Leukocyte Common Antigens