Differentiation of pluripotent stem cells on multiwalled carbon nanotubes.
This paper studies the adhesion, growth, and differentiation of stem cells on carbon nanotube matrices. Glass coverslips were coated with multiwalled carbon nanotube (MWNT) thin films using layer-by-layer self-assembling techniques. Pluripotent P19 mouse embryonal carcinoma stem cells were seeded onto uncoated or MWNT-coated coverslips, and either maintained in an undifferentiated state or induced to differentiate by the addition of retinoic acid. We found that cell adhesion was increased on the MWNT-coated surfaces, and that the expression patterns of some differentiation markers were altered in cells grown on MWNTs. The results suggest that MWNTs will be useful in directing pluripotent stem cell differentiation for tissue engineering purposes.
Holy
Jon
J
Department of Anatomy, Microbiology and Pathology, University of Minnesota School of Medicine-Duluth, Duluth, MN 55812, USA. jholy@d.umn.edu
Perkins
Edward
E
Yu
Xun
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D036103:Nanotechnology / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon