title: Forensic analysis of dog (Canis lupus familiaris) mitochondrial DNA sequences: an inter-laboratory study of the GEP-ISFG working group.
abstract: A voluntary collaborative exercise aiming at the mitochondrial analysis of canine biological samples was carried out in 2006-2008 by the Non-Human Forensic Genetics Commission of the Spanish and Portuguese Working Group (GEP) of the International Society for Forensic Genetics (ISFG). The participating laboratories were asked to sequence two dog samples (one bloodstain and one hair sample) for the mitochondrial D-loop region comprised between positions 15,372 and 16,083 using suggested primers and PCR conditions, and to compare their results against a reference sequence. Twenty-one participating laboratories reported a total of 67.5% concordant results, 15% non-concordant results, and 17.5% no results. The hair sample analysis presented more difficulty to the participants than the bloodstain analysis, with a high percentage (29%) failing to obtain a result. The high level of participation showed the interest of the community in the analysis of dog forensic samples but the results reveal that crucial methodological issues need to be addressed and further training is required in order to respond proficiently to the demands of forensic casework.
authors: Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal. basch@ipatimup.pt
authors: Barbara
authors: B
authors: van Asch
authors: Cristina
authors: C
authors: Albarran
authors: Antonio
authors: A
authors: Alonso
authors: Ramón
authors: R
authors: Angulo
authors: Cíntia
authors: C
authors: Alves
authors: Eva
authors: E
authors: Betancor
authors: Cecilia I
authors: CI
authors: Catanesi
authors: Daniel
authors: D
authors: Corach
authors: Manuel
authors: M
authors: Crespillo
authors: Christian
authors: C
authors: Doutremepuich
authors: Andone
authors: A
authors: Estonba
authors: Ana Teresa
authors: AT
authors: Fernandes
authors: Eugenia
authors: E
authors: Fernandez
authors: Ana Maria
authors: AM
authors: Garcia
authors: Miguel Angel
authors: MA
authors: Garcia
authors: Patricia
authors: P
authors: Gilardi
authors: Rita
authors: R
authors: Gonçalves
authors: Alexis
authors: A
authors: Hernández
authors: Gabriela
authors: G
authors: Lima
authors: Eugênio
authors: E
authors: Nascimento
authors: Marian M
authors: MM
authors: de Pancorbo
authors: David
authors: D
authors: Parra
authors: Maria de Fátima
authors: Mde F
authors: Pinheiro
authors: Elena
authors: E
authors: Prat
authors: Jorge
authors: J
authors: Puente
authors: José Luis
authors: JL
authors: Ramírez
authors: Fernando
authors: F
authors: Rendo
authors: Isabel
authors: I
authors: Rey
authors: Florencia
authors: F
authors: Di Rocco
authors: Anayanci
authors: A
authors: Rodríguez
authors: Andrea
authors: A
authors: Sala
authors: Joel
authors: J
authors: Salla
authors: Juan J
authors: JJ
authors: Sanchez
authors: Dácil
authors: D
authors: Solá
authors: Sandra
authors: S
authors: Silva
authors: José Juan
authors: JJ
authors: Pestano Brito
authors: António
authors: A
authors: Amorim
mesh_terms: D000818:Animals; D001801:Blood Stains; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs; D004586:Electrophoresis; D006197:Hair / Q000378:metabolism; D006801:Humans; D007753:Laboratories; D016133:Polymerase Chain Reaction; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial