Molecular characterization and tissue expression of ovine PSAM6 gene from muscle full-length cDNA library of black-boned sheep.
An ovine PSMA6 gene was obtained from muscle full-length cDNA library of black-boned sheep. The sequences for the PSAM6 gene of Romney sheep and Yunling black goat were also generated in this study. Sequence analysis revealed that nucleotide sequence of this gene was not homologous to any of the known sheep genes, and its open reading frame encodes a protein that contains the putative conserved domain of proteasome subunit alpha type 6 (PSAM6). The nucleotide sequence had higher identity with other animals. However, one mutation of A to G at the site of 383 bp, leading to an amino acid mutation of Asn to Ser, was found only in the black-boned sheep. Tissue expression analysis indicated that this gene was generally expressed in most tissues and differently expressed in tissues of black-boned sheep. This the first report of the ovine PSAM6 gene.
Hu
Hong
H
Yunnan Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, China.
Liu
Yong-Gang
YG
Zhao
Su-Mei
SM
Deng
Wei-Dong
WD
Gao
Shi-Zheng
SZ
D000818:Animals; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006041:Goats / Q000235:genetics; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*
D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex