title: Interleukin-6 -174 G/C promoter polymorphism and nasal polyposis.
abstract: BACKGROUND
  Nasal polyposis is a chronic disease with unknown etiopathogenesis, although inflammatory mechanisms seem to play a role. One of several inflammatory mediators linked to nasal polyposis is Interleukin-6, which has a single nucleotide polymorphism -174 G/C that seems to promote an inflammatory reaction.
  
  
  OBJECTIVE
  To compare the prevalence of the -174 G/C single nucleotide polymorphism between a group of patients with nasal polyposis and a control group.
  
  
  METHODS
  Cross-sectional study with two groups (thirty two patients with nasal polyposis and fifty five controls) to investigate the -174 G/C polymorphism in blood samples. Asthma, aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with forward primer 5'-ATGCCAAGTGCTGAGTCACTA-3' and reverse primer 5'-GGAAAATCCCACATTTGATA-3', amplifying a 226-bp DNA fragment that contained the - 174 position. The amplified fragment can be cleaved by restriction enzyme NlaIII when the -174 position presented the C allele in two fragments of 117 and 109-bp, visualized by electrophoresis, classifying participants in GG, GC and CC.
  
  
  RESULTS
  In the nasal polyposis group, 65.62% of the patients had the GG genotype, while in the control group, 41.82% had two G alleles, a statistically significant difference, with an odds ratio of 2.65.
  
  
  CONCLUSIONS
  The -174 GG genotype was found more frequently in nasal polyposis patients than in controls, when asthma, aspirin intolerance and atopy were excluded.
authors: Department of Otorhinolaryngology and Head and Neck Surgery of São Paulo Federal University (UNIFESP-EPM), São Paulo, SP, Brazil. edumacoto@uol.com.br
authors: Eduardo Macoto
authors: EM
authors: Kosugi
authors: Cínta Meirelles
authors: CM
authors: de Camargo-Kosugi
authors: Luc Louis Maurice
authors: LL
authors: Weckx
authors: Ismael Dale Cotrim
authors: ID
authors: Guerreiro-da-Silva
authors: Luiz Carlos
authors: LC
authors: Gregorio
mesh_terms: D000328:Adult; D000368:Aged; D003430:Cross-Sectional Studies; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D015850:Interleukin-6