title: GammadeltaTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin.
abstract: Gammadelta T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCRdelta gene usage by gammadelta T cells from the uterine epithelium of pregnant sheep. The TCRdelta genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the Vdelta elements used are also expressed in peripheral blood, indicating that gammadeltaTCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analysis of purified, granulated uterine gammadelta T cells revealed the presence of large amounts of the antimicrobial peptide, granulysin. The results of the present study indicate that ovine uterine gammadeltaTCR(+) intraepithelial lymphocytes have the potential to recognise diverse antigens and may have a role in protecting the uterus from infection during pregnancy and parturition. A similar protective role for gammadelta T cells may exist in the human decidua parietalis.
authors: Oxford University Clinical Research Unit, Hanoi, Viet Nam.
authors: Annette
authors: A
authors: Fox
authors: Jill F
authors: JF
authors: Maddox
authors: Mike J
authors: MJ
authors: de Veer
authors: Els N
authors: EN
authors: Meeusen
mesh_terms: D000818:Animals; D004848:Epithelium / Q000276:immunology*; D005260:Female; D011247:Pregnancy; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014599:Uterus / Q000276:immunology*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta