Engineering analysis and development of the spheroid reservoir bioartificial liver.
A significant demand exists for a liver support device such as a Bioartifical Liver (BAL) to treat patients experiencing acute liver failure. This descriptive paper outlines the design and development of two of the key components of the Mayo Spheroid Reservoir Bioartificial Liver (SRBAL) system. One of the components is the multifunctional Spheroid Reservoir and the other is Multi-shelf Rocker. The Spheroid Reservoir provides an environment to support the viability and functionality of the hepatocyte spheroids at very high cell densities. The Spheroid Reservoir is the biologically active component of this extracorporeal liver support device. Since the Spheroid Reservoir is designed to support 200-400 grams of hepatocyte spheroids, a method to quickly produce large quantities of spheroids is required. The Multi-Shelf Rocker fulfills the production requirement by allowing the culturing of up to six liters of hepatocyte suspension in a conventional laboratory incubator. The SRBAL is designed to provide life sustaining liver-like function to patients in acute liver failure.
McIntosh
Malcolm B
MB
Division Engineering, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, USA. mcintosh.malcolm@mayo.edu
Corner
Stephen M
SM
Amiot
Bruce P
BP
Nyberg
Scott L
SL
D000818:Animals; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D008099:Liver / Q000502:physiology* / Q000528:radiation effects*; D019164:Liver, Artificial; D009924:Organ Culture Techniques / Q000295:instrumentation*; D018874:Spheroids, Cellular / Q000166:cytology / Q000502:physiology; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation*