Chlorogenic acid attenuates adhesion molecules upregulation in IL-1beta-treated endothelial cells.
BACKGROUND
Expression of cell adhesion molecules (CAM) on the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process. Chlorogenic acid is a phenolic compound present in coffee, apples, pears, berries, almonds, artichokes, and aubergines. Previous studies have indicated that CA possesses antioxidant activity in vitro.


OBJECTIVE
We investigated the effects of chlorogenic acid and probucol on monocyte-like adhesion, adhesion molecule expression, NF-kappaB translocation and ROS production in IL-1beta-induced human umbilical vein endothelial cells (HUVECs).


RESULTS
According to the results of the MTT assay, we chose 25 and 50 mumol/L to perform the experiments. Chlorogenic acid dose-dependently suppressed IL-1beta-induced mRNA expression of vascular cell adhesion molecule-1, intercellular cell adhesion molecule-1 and endothelial cell selectin. Chlorogenic acid also suppressed the IL-1beta-induced production of ROS. We also observed that chlorogenic acid attenuated or blocked IL-1beta-induced nuclear translocation of nuclear factor-kappaB subunits p50 and p65, which in turn attenuated CAM expression at the transcription level. Furthermore, chlorogenic acid significantly reduced the adhesion of human monocyte cells (U937) to IL-1beta-treated HUVECs in a dose-response manner. These results are similar to that of probucol.


CONCLUSIONS
We conclude that chlorogenic acid exhibit anti-inflammatory effects in HUVECs by inhibition of U937 monocyte-like adhesion, adhesion molecule expression, NF-kappaB translocation, and ROS production. The anti-inflammatory activity of chlorogenic acid in HUVECs suggests that chlorogenic acid could be useful in the prevention of atherosclerosis.
Chang
Weng-Cheng
WC
Graduate Institute of Medical Sciences, Chang Jung Christian University, Tainan, Taiwan.
Chen
Chia-Hsin
CH
Lee
Ming-Fen
MF
Chang
Ted
T
Yu
Ya-Mei
YM
D000975:Antioxidants / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology*; D019040:E-Selectin / Q000235:genetics; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D053583:Interleukin-1beta / Q000494:pharmacology*; D009000:Monocytes / Q000502:physiology; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D011341:Probucol / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
D000975:Antioxidants; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D002726:Chlorogenic Acid; D011341:Probucol