A crucial step in assisted reproduction technology: human embryo selection using metabolomic evaluation.
We present a new methodology to predict embryo viability in assisted reproductive technology (ART) treatments by determining the relative amino acid concentrations in human embryo culture medium on day 3, using high-performance liquid chromatography with mass spectroscopy analysis without derivatization. The model was performed with soft independent modeling of class analogy for the samples from nonpregnancy and pregnancy cases.
Marhuenda-Egea
Frutos Carlos
FC
Department of Agrochemistry and Biochemistry, University of Alicante, Alicante, Spain. frutos@ua.es
Martínez-Sabater
Encarnación
E
Gonsálvez-Alvarez
Rubén
R
Lledó
Belén
B
Ten
Jorge
J
Bernabeu
Rafael
R
D000596:Amino Acids / Q000378:metabolism; D001755:Blastocyst / Q000378:metabolism; D002853:Chromatography, Liquid; D017077:Culture Media, Conditioned / Q000378:metabolism*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D055442:Metabolome; D008954:Models, Biological; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D027724:Reproductive Techniques, Assisted
D000596:Amino Acids; D017077:Culture Media, Conditioned