Microbial conversion of 5-sulfoisophthalic acid into 5-hydroxyisophthalic acid by Ochrobactrum anthropi S9.
5-Hydroxyisophthalic acid-producing microorganisms were isolated from enrichment cultures using 5-sulfoisophthalic acid as a sulfur source. One bacterium, Ochrobactrum anthropi S9, had the highest 5-sulfoisophthalic acid-degrading activity, and stoichiometrically formed 5-hydroxyisophthalic acid, a raw material for polymer synthesis. Under optimum culture conditions, 1.3 mM 5-hydroxyisophthalic acid accumulated in the medium by 60 h. The addition of Na(2)SO(4), L: -methionine or L: -cysteine at 2 mM inhibited the conversion of 5-sulfoisophthalic acid. O. anthropi S9 cells converted 5-sulfoisophthalic acid, benzenesulfonic acid, 3-sulfobenzoic acid, 4-aminobenzenesulfonic acid, naphthalene-1-sulfonic acid and naphthalene-2-sulfonic acid into the corresponding hydroxylated compounds.
Yamada
Mamoru
M
Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan.
Yoshida
Toyokazu
T
Nagasawa
Toru
T
D001673:Biodegradation, Environmental / Q000187:drug effects; D020621:Ochrobactrum anthropi / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010795:Phthalic Acids / Q000737:chemistry / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism; D013455:Sulfur / Q000494:pharmacology; D013997:Time Factors
D010795:Phthalic Acids; D013451:Sulfonic Acids; C037880:5-hydroxyisophthalic acid; D013455:Sulfur; C549093:5-sulfoisophthalic acid