[Identification and determination of major constituents in Polygonum cuspidatum Sieb. et Zucc. by high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry].
A facile method using high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry (HPLC/ESI-IT-TOF MS) has been established for the analysis of multiple constituents in Polygonum cuspidatum Sieb. et Zucc. Six chemical standards including emodin, chrysophanol, physcion, rhein, aloe-emodin and polydatin were studied systematically and their fragmentation pathways were concluded. The methanol extract of Polygonum cuspidatum Sieb. et Zucc. was separated and analyzed by HPLC/ESI-QIT-TOF MS system in negative ion mode. A total of 10 constituents were identified or tentatively characterized with supporting results on the fragmentation pathways of 6 chemical standards and relative references. These constituents are mainly anthraquinones, stilbenes, torachryson and their derivatives, including resveratroloside, polydatin, emodin-8-O-glucoside, resveratrol, torachryson-8-O-glucoside, emodin-1-O-glucoside, torachryson-8-O-(6'-acetyl)glucoside (newly discovered), physcion-8-O-glucoside, physcion-8-O-(6'-acetyl)glucoside (newly discovered) and emodin. It is an extremely simple way by using HPLC/ESI-IT-TOF MS to provide chemical information concerning the constituents in herbal medicines and making the identification results more convinced.
Dong
Jing
J
School of Pharmaceutical Science, Peking University, Beijing, China.
Wang
Hong
H
Wan
Leren
L
Hashi
Yuki
Y
Chen
Shizhong
S
D000880:Anthraquinones / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000032:analysis / Q000302:isolation & purification
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; C518327:aloe emodin; C058229:polydatin; C020491:rhein