title: Proteome organization in a genome-reduced bacterium.
abstract: The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.
authors: European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
authors: Sebastian
authors: S
authors: Kühner
authors: Vera
authors: V
authors: van Noort
authors: Matthew J
authors: MJ
authors: Betts
authors: Alejandra
authors: A
authors: Leo-Macias
authors: Claire
authors: C
authors: Batisse
authors: Michaela
authors: M
authors: Rode
authors: Takuji
authors: T
authors: Yamada
authors: Tobias
authors: T
authors: Maier
authors: Samuel
authors: S
authors: Bader
authors: Pedro
authors: P
authors: Beltran-Alvarez
authors: Daniel
authors: D
authors: Castaño-Diez
authors: Wei-Hua
authors: WH
authors: Chen
authors: Damien
authors: D
authors: Devos
authors: Marc
authors: M
authors: Güell
authors: Tomas
authors: T
authors: Norambuena
authors: Ines
authors: I
authors: Racke
authors: Vladimir
authors: V
authors: Rybin
authors: Alexander
authors: A
authors: Schmidt
authors: Eva
authors: E
authors: Yus
authors: Ruedi
authors: R
authors: Aebersold
authors: Richard
authors: R
authors: Herrmann
authors: Bettina
authors: B
authors: Böttcher
authors: Achilleas S
authors: AS
authors: Frangakis
authors: Robert B
authors: RB
authors: Russell
authors: Luis
authors: L
authors: Serrano
authors: Peer
authors: P
authors: Bork
authors: Anne-Claude
authors: AC
authors: Gavin
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D019295:Computational Biology; D016680:Genome, Bacterial; D013058:Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways; D008854:Microscopy, Electron; D008954:Models, Biological; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000032:analysis* / Q000378:metabolism; D009177:Mycoplasma pneumoniae / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010363:Pattern Recognition, Automated; D025941:Protein Interaction Mapping; D020543:Proteome; D049490:Systems Biology
chemical_list: D001426:Bacterial Proteins; D046912:Multiprotein Complexes; D020543:Proteome