title: Photophysical properties of a new DyLight 594 dye.
abstract: We describe spectral properties of novel fluorescence probe DyLight 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.
authors: Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
authors: Pabak
authors: P
authors: Sarkar
authors: Savitha
authors: S
authors: Sridharan
authors: Rafal
authors: R
authors: Luchowski
authors: Surbhi
authors: S
authors: Desai
authors: Boguslawa
authors: B
authors: Dworecki
authors: Marie
authors: M
authors: Nlend
authors: Zygmunt
authors: Z
authors: Gryczynski
authors: Ignacy
authors: I
authors: Gryczynski
mesh_terms: D000042:Absorption; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence
chemical_list: C417664:Alexa594; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D009930:Organic Chemicals