Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed.
Methods for transfer of exogenous DNA into cells are essential for genetics and molecular biology, and the lack of effective methods hampers research on many different species of bacteria which have shown to be particularly recalcitrant to transformation. This review presents the progress on the development of methods for artificial transformation of bacteria with emphasis on different methodologies and the range of bacteria that can be transformed. The methods' strengths and weaknesses are described.
Aune
Trond Erik Vee
TE
Vectron Biosolutions AS, Pirsenteret, 7462 Trondheim, Norway.
Aachmann
Finn Lillelund
FL
D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005818:Genetic Engineering; D009154:Mutation; D010641:Phenotype; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004269:DNA, Bacterial