title: SIZ1, a small ubiquitin-related modifier ligase, controls cold signaling through regulation of salicylic acid accumulation.
abstract: Low temperature induces several genes to acquire plant cold tolerance. Here, we demonstrate that accumulation of salicylic acid (SA) is involved in the regulation of the DREB1A/CBF3 regulon and plant tolerance to cold stresses. The SA-accumulating mutant siz1 exhibits sensitivity to chilling and freezing conditions and decreased expression of DREB1A/CBF3 and its regulon genes. Reduction of SA levels in siz1 by nahG restored cold sensitivity and down-regulation of these genes. Database analyses and RT-PCR analysis revealed that the ice1 mutation also increased expression of SA-responsive genes. As well as siz1, another SA-accumulating mutant acd6 exhibited freezing sensitivity and the sensitivity was suppressed in acd6 nahG plants. Taken together, these data indicate that SA is involved in regulation of cold signaling.
authors: Graduate School of Life and Environmental Sciences, Initiative for the Promotion of Young Scientists' independent Research, University of Tsukuba, Gene Research Center 220, 1-1-1 Ten-nou dai, Tsukuba 305-8572, Japan. kmiura@gene.tsukuba.ac.jp
authors: Kenji
authors: K
authors: Miura
authors: Masaru
authors: M
authors: Ohta
mesh_terms: D000064:Acclimatization; D017487:Ankyrins / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C480953:ACD6 protein, Arabidopsis; D017487:Ankyrins; D029681:Arabidopsis Proteins; C510502:ICE1 protein, Arabidopsis; D014157:Transcription Factors; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase; D008025:Ligases; C502552:SIZ1 protein, Arabidopsis; D020156:Salicylic Acid