[Inhibition of tumour cells with hepatitis B virus x (HBx) gene adenoviral vector in vivo].
OBJECTIVE
To investigate the inhibitory effects of the hepatitis B virus x protein (HBx protein) on tumor in vivo.


METHODS
H22 cells were infected with Ad-eGFP to detect infection efficiency of adenovirus. The BALB/c mouse model of malignant ascites was established by implanting H22 cell intraperitoneally into the animal, Ad-HBx, Ad-null or NS were administered intraperitoneally in BALB/c mice separately to detect HBx expression in H22 cells and effects of HBx on inhibition on proliferation of H22 cells.


RESULTS
High efficiency of Ad in infecting H22 cells in vitro was observed. HBx protein was expressed in H22 cells after intraperitoneal injection of Ad-HBx. The effect of Ad-HBx on inhibition of the peritoneal capillary permeability and the ascites accumulation (P<0.05); on reduction of the number of red cells and tumor cells counted in malignant ascites (P<0.05), and on inhibition of tumor cell life cycle (the G2/M arrest) in malignant ascites were identified by flow cytometric analysis.


CONCLUSIONS
HBx protein can be expressed in tumour cells and can inhibit the proliferation of tumour cells in vivo, and this might be a new potential treatment for malignant ascites.
Lü
Hui-Min
HM
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Cheng
Ping
P
Tang
Qing-Qing
QQ
Chen
Ping
P
Peng
Xing-Chen
XC
Li
Yu-Hua
YH
Wen
Yan-Jun
YJ
Wei
Yu-Quan
YQ
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D011994:Recombinant Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C485184:enhanced green fluorescent protein; C057628:hepatitis B virus X protein; D049452:Green Fluorescent Proteins