title: Optimization of 1-deoxynojirimycin extraction from mulberry leaves by using response surface methodology.
abstract: Mulberry 1-deoxynojirimycin (DNJ, a potent alpha-glycosidase inhibitor) has therapeutic potency against diabetes mellitus. However, the amount of DNJ in mulberry leaves is low (about 0.1%), and a more effective extraction method is needed. Ultrasound-assisted extraction (UAE) was applied in this study for mulberry DNJ extraction, and five factors, the percentage of ethanol in the extraction solvent (x(1)), ratio of the extraction solvent to mulberry sample (x(2)), ultrasonic power (x(3)), extraction temperature (x(4)) and extraction time (x(5)), were investigated by fractional factorial 2((5-1)) design (FFD) to obtain the optimum extraction efficiency (DNJ yield, Y(1)) and extraction productivity (total yield, Y(2)). The results showed that x(2), x(3) and x(5) had significant impact on Y(1) and Y(2), and were further optimized by response surface methodology (RSM). Under the optimized conditions (x(2), x(3) and x(5) of 7 ml/g, 180 W and 260 s, respectively), DNJ-enriched powder (0.8%) was produced with high extraction efficiency (98%) and productivity (20%), enabling this product to be used for nutraceutical purposes.
authors: Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Chaluntorn
authors: C
authors: Vichasilp
authors: Kiyotaka
authors: K
authors: Nakagawa
authors: Phumon
authors: P
authors: Sookwong
authors: Yasuyo
authors: Y
authors: Suzuki
authors: Fumiko
authors: F
authors: Kimura
authors: Ohki
authors: O
authors: Higuchi
authors: Teruo
authors: T
authors: Miyazawa
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D002845:Chromatography; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014465:Ultrasonics
chemical_list: D017485:1-Deoxynojirimycin