[Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development].
OBJECTIVE
To study on the expression pattern of a novel gene, Biot2-L, in mice and its primary bio-function on the development of testis.


METHODS
Bioinformatics analysis were applied to identify the structure characteristics and homologene of Biot2-L. RT-PCR and Real-time RT-PCR were used to analyze its expression profile in normal tissues and the time-dependent expression pattern during the testes sexual maturation.


RESULTS
The complete sequence of Biot2-L was obtained. A conserved domain-CCDC7 was detected and there were homolog proteins in advanced mammals with about 50% identity by bioinformatics analysis. The highly expression of Biot2-L in adult testis but low or absent expressed in other tissues was observed. A regular change of Biot2-L expression level during the testis development and spermatogonia was demonstrated by the results of Real-time RT-PCR. The mRNA transcript appeared from the fifth week of postnatal development, and the expression level increased up to adulthood, but decreased when get older.


CONCLUSIONS
Biot2-L was highly expressed in adult testis and regular changed during the testis development, which suggested that the Biot2-L would be involved in the testis development and spermatogonia.
Wang
Hao
H
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Zhang
Peng
P
Wang
Chun-Ting
CT
D000818:Animals; D000831:Animals, Newborn; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013737:Testis / Q000254:growth & development* / Q000378:metabolism*
D000951:Antigens, Neoplasm; C543854:Ccdc7b protein, mouse