GCS induces multidrug resistance by regulating apoptosis-related genes in K562/AO2 cell line.
We have previously shown that the expression of glucosylceramide synthase (GCS) gene in drug-resistant K562/AO2 human leukemia cell was higher than that in drug-sensitive K562 cell, and the sensitivity to adriamycin of K562/AO2 cell was enhanced by inhibiting GCS. It is concluded that the overexpression of GCS gene is one of the reasons which lead to multidrug resistance (MDR) of leukemia cell. Meanwhile, we also found that higher expression of Bcl-2 gene and protein were exhibited in K562/AO2 cell compared with K562 cell. Basing on this, we hypothesized that the high expression of GCS gene which results in MDR of leukemia cell is correlated with Bcl-2 signal transduction. In order to validate the hypothesis, the inhibition of GCS gene in K562/AO2 cell was observed by using chemical suppressor PPMP and siRNA targeted at GCS, and applying RT-PCR and flow cytometry, the expression levels of apoptosis-related gene Bcl-2 and Bax were analyzed before and after inhibiting GCS gene in K562/AO2 cell. The results demonstrated that the gene and protein of Bcl-2 in K562/AO2 cell were both down-regulated significantly after GCS gene being inhibited; however, the Bax mRNA expression had no apparent change in different groups. This suggested that GCS gene may contributed to MDR of human leukemia cell K562/AO2 by Bcl-2 signal transduction.
Liu
Yan
Y
Wuxi Higher Health Vocational Technology School, Wuxi, Jiangsu, China.
Xie
Ke-Ming
KM
Yang
Guo-Qing
GQ
Bai
Xiao-Ming
XM
Shi
Yuan-Ping
YP
Mu
Hui-Jun
HJ
Qiao
Wei-Zhen
WZ
Zhang
Bin
B
Xie
Ping
P
D017209:Apoptosis / Q000235:genetics*; D015536:Down-Regulation; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D005964:Glucosyltransferases / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D009025:Morpholines / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger; D034741:RNA, Small Interfering / Q000008:administration & dosage; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013107:Sphingolipids / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000235:genetics
C095823:1-phenyl-2-palmitoylamino-3-morpholino-1-propanol; D009025:Morpholines; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013107:Sphingolipids; D051028:bcl-2-Associated X Protein; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase