title: Cryptosporidium parvum in Korea: prevalence in individuals residing in three major river valleys and genetic characteristics of the isolates.
abstract: Cryptosporidiosis is a diarrheal illness caused by apicomplexa parasite Cryptosporidium spp. In this study, to examine the overall infection status of Cryptosporidium spp. in individuals residing in southern parts of Korea, eight counties around Yeongsan, Seomjin and Nakdong River valleys was surveyed. The investigation was carried out from April to October 2005. A total of 9,498 stool samples were collected from individuals. Stool samples were analyzed for modified acid-fast stains, and DNA fragment extracted from positive samples was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 18S rRNA polymorphic region. Oocysts of Cryptosporidium spp. were detected in 239 specimens (2.5%) by a modified acid-fast stain. Infection rate was not significantly different between male (2.2%) and female (2.8%) individuals examined (P>0.05). In the infection rate by age, totally 1-9 (4.8%) and 80< (3.7%) age group were shown to the highest, and there was shown to significant differences (P<0.05). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rRNA gene from 51 isolates showed that all the isolates were identified as C. parvum. Our data collectively suggested that C. parvum infection is prevalent in the studied areas of Korea and more comprehensive nation-wide epidemiological studies are needed to elucidate the infection status of Cryptosporidium infection in Korea.
authors: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul 122-701, Korea. ilcheun7@korea.kr
authors: Hyeng-Il
authors: HI
authors: Cheun
authors: Shin-Hyeong
authors: SH
authors: Cho
authors: Yi-Young
authors: YY
authors: Lim
authors: Byung-Chul
authors: BC
authors: Lee
authors: Jung-Yeon
authors: JY
authors: Kim
authors: Jung-Won
authors: JW
authors: Ju
authors: Byoung-Kuk
authors: BK
authors: Na
authors: Isao
authors: I
authors: Kimata
authors: Jae-Ran
authors: JR
authors: Yu
authors: Tong-Soo
authors: TS
authors: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007223:Infant; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D012424:Rural Population; D012737:Sex Factors; D055815:Young Adult; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S