title: The effect of retroviral vector on uptake of human lactoferrin DNA by Yak (Bos Grunniens) spermatozoa and their fertilizability in vitro.
abstract: The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using PLNCX2-hLF+FuGene 6 to generate SMGT yak spermatozoa for fertilizing bovine oocytes. The result showed that DNA-binding rate increased with the extension of incubation period and DNA treatment did not decrease sperm motility. Oocytes inseminated with SMGT-spermatozoa had a lower (P < 0.05) cleavage rate (57.7%) than the control (73.4%), but development up to blastocyst stage was not different (26.8 vs. 31.7%). It appears that PLNCX2 is useful for generating transgenic yaks by SMGT.
authors: The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities, Chengdu, China. zixd2000@yahoo.com
authors: Xiang-Dong
authors: XD
authors: Zi
authors: Shao-Wei
authors: SW
authors: Chen
authors: Guan-Nan
authors: GN
authors: Liang
authors: Da-Wen
authors: DW
authors: Chen
authors: Da-Wei
authors: DW
authors: Zhang
authors: Rong-Hua
authors: RH
authors: Yin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D004247:DNA / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008297:Male; D012190:Retroviridae / Q000235:genetics*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*
chemical_list: D004247:DNA; D007781:Lactoferrin