title: [Gastric cancer detection using kubelka-Munk spectral function of DNA and protein absorption bands].
abstract: Differential diagnosis for epithelial tissues of normal human gastric, undifferentiation gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 250 to 650 nm, pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the DNA absorption bands at 260 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer. Their differences were 68.5% (p < 0.05), 146.5% (p < 0.05), 282.4% (p < 0.05), 32.4% (p < 0.05), 56.00 (p < 0.05) and 83.0% (p < 0.05) respectively. And pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the protein absorption bands at 280 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer. Their differences were 86.8% (p < 0.05), 262.9% (p < 0.05), 660.1% (p < 0.05) and 34% (p < 0.05), 72. 2% (p < 0.05), 113.5% (p < 0.05) respectively. And pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the carotene absorption bands at 480 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer. Their differences were 59.5% (p < 0.05), 73% (p < 0.05), 258.9% (p < 0.05), 118.7% (p < 0.05), 139.2% (p < 0.05), and 324. 6% (p < 0.05) respectively. It is obvious that pathological changes of gastric epithelial tissues induced that there were significant changes in the contents of the DNA, protein and beta-carotene of gastric epithelial tissues. The conclusion can be applied to rapid, low-cost and noninvasive the optical biopsy for gastric cancer and provides a useful reference.
authors: Key Laboratory of Laser Life Science and Institute of Laser Life Science of Ministry of Education, South China Normal University, Guangzhou 510631, China. lilanq@yahoo.cn
authors: Lan-quan
authors: LQ
authors: Li
authors: Hua-jiang
authors: HJ
authors: Wei
authors: Zhou-yi
authors: ZY
authors: Guo
authors: Hong-qin
authors: HQ
authors: Yang
authors: Shu-sen
authors: SS
authors: Xie
authors: Xue-mei
authors: XM
authors: Chen
authors: Li-bo
authors: LB
authors: Li
authors: Bol-hua
authors: BH
authors: He
authors: Guo-yong
authors: GY
authors: Wu
authors: Jian-jun
authors: JJ
authors: Lu
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis; D004247:DNA; D005753:Gastric Mucosa / Q000473:pathology*; D006801:Humans; D011506:Proteins; D013053:Spectrophotometry; D013274:Stomach Neoplasms / Q000175:diagnosis*; D019207:beta Carotene
chemical_list: D011506:Proteins; D019207:beta Carotene; D004247:DNA