[Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus].
OBJECTIVE
Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus.


METHODS
Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains.


RESULTS
33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%.


CONCLUSIONS
MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.
Wang
Xia
X
Wuhan Institute of Biological Products, Wuhan, China.
Xu
Ge-Lin
GL
Wu
Jie
J
Luo
Jing
J
Zhu
Jia-Hong
JH
Zhou
Jian-Jun
JJ
Shi
Nan-Feng
NF
Tang
Qing
Q
Zhang
Yong-Zhen
YZ
Tang
Jian-Rong
JR
Wu
Tai-Cai
TC
Ming
He-Tian
HT
Shen
Rui-Hua
RH
Chen
Huan-Chun
HC
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005240:Feasibility Studies; D005455:Fluorescent Antibody Technique / Q000379:methods; D051379:Mice; D011820:Rabies virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity