Controlled cholesterol efflux from the aortic smooth muscle cells triggers microheterogeneity of plasma membrane lipids and induces modification of the mitochondrial topology.
It is generally accepted that phospholipids of plasma membrane display lateral segregation into small microdomains commonly known as lipid rafts. Such lateral lipid organization is under the control of cholesterol. Cholesterol depletion evolved by methyl-beta-cyclodextrin (MCD) has been found to induce further marked perturbation in lateral lipid organization, evidenced in the high field part of electron paramagnetic resonance spectra of plasma membranes labelled with a spectroscopic probe, namely 5-doxyl-stearic acid (5DOXS). Such perturbation of surface lipid topo-logy has been found to induce distinct changes in the mitochondrial morpho-logy, i.e. switch from filamentous form into small granular form.
Tukaj
C
C
Department of Electron Microscopy, Medical University of Gdańsk, Debinki 1, 80-211 Gdánsk, Poland. ctukaj@amedec.amg.gda.pl
Syta-Ksiazek
E
E
Zauszkiewicz
A
A
Drózd
K
K
Figarski
A
A
Spodnik
J H
JH
Woźniak
M
M
D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D047392:beta-Cyclodextrins / Q000737:chemistry*
D003497:Cyclic N-Oxides; D010743:Phospholipids; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C005072:5-doxylstearic acid; D002784:Cholesterol