title: [The value of regulated upon activation normal T cell expressed and secreted in the pathogenesis of varicose ulcer of lower limbs].
abstract: OBJECTIVE
  To study the effectiveness of RANTES in the pathogenesis of venous ulceration.
  
  
  METHODS
  40 Chronic venous insufficiency (CVI) individuals were enrolled in the study and separated into the ulceration group (n=20) and non-ulceration group (n=20), according to CVI with or without ulceration, 10 healthy individuals were enrolled as control group. The expression of RANTES mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in blood. Immunohistochemical staining of RANTES were carried in normal skin, in the tissue at the brim of ulceration and in the tissue of cured or curing ulceration.
  
  
  RESULTS
  In ulceration group, the expression of RANTES mRNA in blood increased obviously. Compared with the other two groups, they were significant different (P < 0.05). The difference was also significance in the other two groups (P < 0.05). The average number of RANTES positive expression in normal skin is 28 +/- 13, in the tissue at the brim of ulceration is 107 +/- 44, and in the tissue of cured or curing ulceration is 35 +/- 20. There are significant difference between normal skin and the tissue at the brim of ulceration (P < 0.05). The average number of RANTES positive expression in the tissue of cured or curing ulceration is lower than that's in the tissue at the brim of ulceration.
  
  
  CONCLUSIONS
  The high expression of RANTES may be one of the important mechanisms of varicose ulcer.
authors: Vascular Surgery Center of Sun Yat-sen University, Department of Vascular Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors: Liu-hu
authors: LH
authors: Chen
authors: Da-yue
authors: DY
authors: Liu
authors: Jiang
authors: J
authors: Xiong
authors: Cai-sheng
authors: CS
authors: Ye
authors: Heng-hui
authors: HH
authors: Yin
authors: Shen-ming
authors: SM
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000378:metabolism*; D005260:Female; D006801:Humans; D035002:Lower Extremity / Q000098:blood supply; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D014647:Varicose Ulcer / Q000378:metabolism* / Q000473:pathology; D014689:Venous Insufficiency / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D018946:Chemokine CCL5; D012333:RNA, Messenger