Basic fibroblast growth factor combined with atelocollagen for closing chronic tympanic membrane perforations in 87 patients.
OBJECTIVE
To present the clinical results of closing chronic tympanic membrane (TM) perforations using basic fibroblast growth factor (bFGF) combined with an atelocollagen/silicone bilayer membrane patch.


METHODS
Closure of TM perforations in 87 patients was attempted using bFGF, which is thought to promote the regeneration of TM tissues by facilitating the growth of fibroblasts and collagen fibers.


METHODS
Under an operating microscope, the margin of the perforation was trimmed, and a piece of an atelocollagen/silicone bilayer membrane was placed in the perforation with the silicon layer facing outward and then infiltrated with 0.1 ml of trafermin. Data obtained from patient records included patient age, perforation size, and duration of treatment, with a focus on hearing improvement and complete TM closure.


RESULTS
The mean perforation size before treatment was 14.4%. Complete closure of the TM perforation was achieved in 80 patients (92.0%), whereas pinholes remained in 5 patients (8.7%), and small perforations were observed in 2 patients (2.3%). In the patients with complete closure, the TM perforations closed after an average 1.8 treatments, and hearing improved by 13.6 dB.


CONCLUSIONS
This study demonstrated that bFGF combined with atelocollagen is effective for the conservative treatment of TM perforation.
Hakuba
Nobuhiro
N
Department of Otolaryngology-Head and Neck Surgery, Osaka Red Cross Hospital, Osaka, Japan. hakuba@m.ehime-u.ac.jp
Iwanaga
Michitaka
M
Tanaka
Shinzo
S
Hiratsuka
Yasuyuki
Y
Kumabe
Yohei
Y
Konishi
Masaya
M
Okanoue
Yusuke
Y
Hiwatashi
Nao
N
Wada
Tadahiko
T
D001309:Auditory Threshold; D002908:Chronic Disease; D003094:Collagen / Q000627:therapeutic use*; D005260:Female; D005346:Fibroblast Growth Factors / Q000627:therapeutic use*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009225:Myringoplasty / Q000295:instrumentation* / Q000379:methods; D010446:Peptide Fragments / Q000627:therapeutic use*; D020127:Recovery of Function; D014014:Tissue Adhesives / Q000627:therapeutic use*; D016896:Treatment Outcome; D018058:Tympanic Membrane Perforation / Q000601:surgery*; D014945:Wound Healing
D010446:Peptide Fragments; D014014:Tissue Adhesives; C059821:atelocollagen; C114444:trafermin; D005346:Fibroblast Growth Factors; D003094:Collagen