Novel multifunctional polyethylene glycol-transactivating-transduction protein-modified liposomes cross the blood-spinal cord barrier after spinal cord injury.
The blood-spinal cord barrier (BSCB) prevents many macromolecular agents from passing through to reach sites of injury in the spinal cord. This study evaluated the ability of a novel multifunctional liposome modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) containing an iron core to cross the BSCB using a rat model of spinal cord injury. Rats were examined daily for a period of three days after spinal cord injury and injection of either the multifunctional modified liposome or control formulations using a 3.0 T magnetic resonance imaging spectrometer. A low signal was observed in the T2-weighted images. Prussian blue staining and flame atomic absorption spectrophotometry revealed that significantly more iron accumulated around the lesion site in the experimental group than the control groups (P < 0.05). The findings from this study suggest that this multifunctional liposome carrier can cross the BSCB to accumulate around the lesion site.
Liu
Yang
Y
Department of Orthopaedics, Tianjin Medical University General Hospital, Anshan Road 154, Tianjin, PR China.
Wang
Chun-Yuan
CY
Kong
Xiao-Hong
XH
Wang
Han-Jie
HJ
Chang
Jin-
J
Zhang
Da-Peng
DP
Ban
De-Xiang
DX
Feng
Shi-Qing
SQ
D000818:Animals; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics; D002784:Cholesterol / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000379:methods*; D015696:Gene Products, tat / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D007501:Iron / Q000737:chemistry; D008081:Liposomes; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010316:Particle Size; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D013054:Spectrophotometry, Atomic; D013116:Spinal Cord / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D013119:Spinal Cord Injuries / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013499:Surface Properties
D015696:Gene Products, tat; D008081:Liposomes; D010446:Peptide Fragments; D010455:Peptides; D011092:Polyethylene Glycols; D048271:Chitosan; D002784:Cholesterol; D007501:Iron