title: PGP9.5 was less frequently methylated in advanced gastric carcinoma.
abstract: OBJECTIVE
  Recently, it was examined the methylation status of the Protein Gene Product 9.5 (PGP9.5) gene in primary tumors derived from 49 patients with colorectal cancer and evaluated the correlation between the methylation status and the clinicopathological findings. A significant difference was observed in lymph node metastasis (p = 0.029), suggesting that PGP9.5 was less frequently methylated in metastatic colorectal cancer. This result prompted us to examine the methylation status of the PGP9.5 gene in gastric cancers.
  
  
  METHODS
  It was examined the methylation status of the PGP9.5 gene in primary tumors derived from 30 patients with gastric cancer using qMSP and evaluated the correlation between the methylation status and the clinicopathological findings.
  
  
  RESULTS
  Aberrant methylation of the PGP9.5 gene was detected in 5 of 30 (17%) primary gastric cancers. The present results suggested that the aberrant methylation of the PGP9.5 gene was frequently observed in gastric cancers. Subsequently, clinicopathological data were correlated with the methylation results. A significant difference was observed in extent of tumor, lymph node metastasis, and TNM stage (p = 0.034, 0.015, and 0.028, respectively).
  
  
  CONCLUSIONS
  PGP9.5 was less frequently methylated in advanced gastric cancer compared to earlier one.
authors: Department of Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama 227-8501, Japan.
authors: Hirohi
authors: H
authors: Mizukami
authors: Tetsuhiro
authors: T
authors: Goto
authors: Youhei
authors: Y
authors: Kitamura
authors: Makiko
authors: M
authors: Sakata
authors: Mitsuo
authors: M
authors: Saito
authors: Kazuyoshi
authors: K
authors: Ishibashi
authors: Gaku
authors: G
authors: Kigawa
authors: Hiroshi
authors: H
authors: Nemoto
authors: Yutaka
authors: Y
authors: Sanada
authors: Kenji
authors: K
authors: Hibi
mesh_terms: D000368:Aged; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D043222:Ubiquitin Thiolesterase / Q000235:genetics*
chemical_list: C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase