title: Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice.
abstract: The purpose of this study was to investigate the potential of intranasal (IN) immunization with Neisseria meningitides B proteoliposome (AFPL1) and AFPL1-derived cochleate (AFCo1), containing glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) for induction of protective immunity against genital herpes infection in mice. We could show that IN immunization with both AFPL1 and AFCo1 containing gD induced gD-specific IgG antibody and lymphoproliferative responses. However, IFN-gamma response could only be detected in CD4(+) splenic cells and genital lymph node cells of the AFCo1gD immunized mice upon recall antigen stimulation in vitro. Importantly, IN immunization with AFCo1gD could elicit a complete protection against an otherwise lethal vaginal challenge with HSV-2, while the AFPL1gD immunized mice were only partially protected. Further, we could show that the IFN-gamma response and protective immunity observed after IN immunization with AFCo1gD are mediated via the adaptor molecule myeloid differentiation factor 88. These data may have implications for the development of a mucosal vaccine against genital herpes.
authors: Department of Immunology, Finlay Institute, Havana, Cuba.
authors: Judith
authors: J
authors: Del Campo
authors: Madelene
authors: M
authors: Lindqvist
authors: Maribel
authors: M
authors: Cuello
authors: Malin
authors: M
authors: Bäckström
authors: Osmir
authors: O
authors: Cabrerra
authors: Josefine
authors: J
authors: Persson
authors: Oliver
authors: O
authors: Perez
authors: Ali M
authors: AM
authors: Harandi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D006558:Herpes Genitalis / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008081:Liposomes; D051379:Mice; D018345:Mice, Knockout; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000276:immunology; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*
chemical_list: D000914:Antibodies, Viral; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008081:Liposomes; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2; D007371:Interferon-gamma