title: Molecular identification of enteroviruses including two new types (EV-98 and EV-107) isolated from Japanese travellers from Asian countries.
abstract: Of 58 enterovirus strains isolated from Japanese travellers returning from Asian countries, eight were non-serotypable with existing antisera. By sequencing a part of the VP1 region, six of these strains were typed as echovirus 9, enterovirus (EV)-73, EV-79 or EV-97. The nucleotide identity of the VP1 region of isolate T92-1499 to all enterovirus prototypes was <70 %. The VP1 sequence of isolate TN94-0349 was closely related to coxsackievirus (CV)-A9 (73.3 % nucleotide identity), but the virus could not be neutralized with a serum raised against the prototype CV-A9 strain. On the basis of complete molecular comparisons, T92-1499 and TN94-0349 were identified as EV-98 and EV-107, respectively, by the ICTV Picornavirus Study Group. Serum neutralization tests of Japanese individuals revealed a seroprevalence rate of 11 % for EV-73, and even lower seroprevalence rates, 1.0-3.8 %, were found for the other new enteroviruses, suggesting that prior circulation of these viruses in Japan was unlikely.
authors: Laboratory of Virology, Department of Microbiology and Medical Zoology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. tyamashita@hi-ho.ne.jp
authors: Teruo
authors: T
authors: Yamashita
authors: Miyabi
authors: M
authors: Ito
authors: Hideaki
authors: H
authors: Tsuzuki
authors: Kenji
authors: K
authors: Sakae
authors: Hiroko
authors: H
authors: Minagawa
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007223:Infant; D007564:Japan; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D014195:Travel
chemical_list: D000914:Antibodies, Viral