Estrogen withdrawal transiently increased bone turnover without affecting the bone balance along the tooth socket in rats.
BACKGROUND
Estrogen withdrawal causes marked bone loss in the appendicular skeleton but slightly affects mandibular cancellous bone; in contrast, little is known of its effects on alveolar wall turnover associated with tooth drift. In this study, we assessed short-term changes in alveolar wall turnover after an ovariectomy and compared it to other bone sites exhibiting different levels of turnover.


METHODS
Forty Sprague-Dawley rats were ovariectomized or sham operated. Right mandibles and femurs were processed without demineralization for bone histomorphometry in three different sites: the alveolar wall around the first molar buccal root, apical interradicular bone, and femoral metaphysis. Bone changes were assessed 14 and 28 days after the ovariectomy. Data were compared using non-parametric statistics.


RESULTS
At 14 days, on the resorption side of the alveolar wall, resorption parameters were higher in the ovariectomized rats (P <0.01), whereas the formation was lower (P <0.05); on the formation side, the daily mineral apposition rate increased (P <0.01). The root resorption was higher in ovariectomized rats (P <0.05). In the periodontal ligament, the numbers of osteoclast precursors were significantly higher. At 28 days, the drift slowed down in both the sham and ovariectomized groups. The ovariectomy had no effect on interradicular bone turnover, whereas bone loss and numbers of osteoclasts were strongly increased in the femur as soon as 14 days after the ovariectomy.


CONCLUSIONS
Estrogen withdrawal had transient repercussions on alveolar wall turnover. The different reactivities of the three envelopes studied suggest that a response to an ovariectomy in the short term is related to initial basal turnover.
Gallina
S
S
Orofacial Repair and Remodelings, Faculty of Odontology, Paris Descartes University, Montrouge, France.
Barranco-Piedra
S
S
Torres-Lagares
D
D
Baroukh
B
B
Llorens
A
A
Gutiérrez-Pérez
J-L
JL
Saffar
J-L
JL
Cherruau
M
M
Lesclous
P
P
D000135:Acid Phosphatase / Q000032:analysis; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D016723:Bone Remodeling / Q000502:physiology*; D001862:Bone Resorption / Q000473:pathology; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D005269:Femur / Q000473:pathology; D007527:Isoenzymes / Q000032:analysis; D008334:Mandible / Q000473:pathology; D008963:Molar / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010052:Ovariectomy; D010513:Periodontal Ligament / Q000473:pathology; D053245:RANK Ligand / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012391:Root Resorption / Q000473:pathology; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D020390:Tooth Socket / Q000473:pathology*; D000097382:CD68 Molecule
D000135:Acid Phosphatase; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D004967:Estrogens; D007527:Isoenzymes; D053245:RANK Ligand; D000071681:Tartrate-Resistant Acid Phosphatase; C539355:CD68 protein, rat; D000097382:CD68 Molecule