title: [Human hnRNP I prokaryotic expression and preliminary application.].
abstract: OBJECTIVE
  To express recombinant protein hnRNP I with prokaryotic expression system and assess the presence of autoantibodies against hnRNP I in systemic sclerosis (SSc) as well as other CTD.
  
  
  METHODS
  Human hnRNP I gene was amplified by RT-PCR from HeLa cells and cloned into pET-30a vector , then pET-30a-hnRNP I plasmid was transferred into E.coli BL21 (DE3) to express recombinant protein. The sera from patients including SSc, SLE, SS, MCTD, UCTD, RA and controls were detected by ELISA with the purified recombinant hnRNP I protein.
  
  
  RESULTS
  The recombinant protein was highly expressed in E.coli BL21(DE3) and specially reacted with clinically diagnosed SSc patients's serum. The result suggested that the autoantibodies against hnRNP I had higher positive ratio(48.72%) in SSc than other serum of AID and control.
  
  
  CONCLUSIONS
  Human hnRNP I protein was successfully expressed in prokaryotic expression system. The purified hnRNP I protein can be used to diagnosis of SSc.
authors: Department of Immunology, Weifang Medical University; Key Laboratory of Immunology in Universities of Shandong, Weifang 261042, China.
authors: Cheng-Tao
authors: CT
authors: Sun
authors: Fan
authors: F
authors: Feng
authors: Lei
authors: L
authors: Wang
authors: Pei-Hong
authors: PH
authors: Liu
authors: Dong-Zhen
authors: DZ
authors: Mu
authors: Ji-Yu
authors: JY
authors: Ju
mesh_terms: D001323:Autoantibodies / Q000097:blood; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D001323:Autoantibodies; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D011994:Recombinant Proteins