title: Congenital imperforate hymen with hydrocolpos and hydronephrosis associated with severe hydramnios and increase of maternal ovarian steroidogenic enzymes.
abstract: OBJECTIVE
  To study clinical features of patient presented with severe hydramnios, associated with hydronephrosis, that was antenatally diagnosed and has been successfully treated immediately after birth. At a molecular level, we investigated the gene expression of key steroidogenic enzymes from the maternal ovary.
  
  
  METHODS
  Ultrasound scan, MRI, semi-quantitative RT-PCR SETTING: The patient was admitted to the University Hospital, University of Crete, Medical School, Greece, where all clinical data has been obtained. Gene expression studies took place at Biosciences, Brunel University, UK.
  
  
  RESULTS
  Semi-quantitative RT-PCR analyses revealed that there is upregulation of key steroidogenic genes in the maternal ovary, including steroidogenic acute regulatory protein, and the cytochrome P450 heme-containing proteins CYP11A, CYP17 and CYP19. From a clinical perspective, the prenatal ultrasound scan and MRI findings showed a multicystic pelvic mass, bilateral hydronephrosis and prior to delivery severe polyhydramnios.
  
  
  CONCLUSIONS
  This clinical case is the only one that we have found in the current literature where congenital imperforate hymen accompanied with hematocolpos is associated with renal obstruction in combination with polyhydramnios and increase in maternal steroidogenic enzymes.
authors: Centre for Cell Chromosome Biology, Biosciences, School of Health Sciences and Social Care, Brunel University, Uxbridge, UK.
authors: Emmanouil
authors: E
authors: Karteris
authors: Helen
authors: H
authors: Foster
authors: Maria
authors: M
authors: Karamouti
authors: Anastasia
authors: A
authors: Goumenou
mesh_terms: D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D052202:Hydrocolpos / Q000201:enzymology*; D006869:Hydronephrosis / Q000000981:diagnostic imaging / Q000201:enzymology*; D006924:Hymen / Q000002:abnormalities*; D007231:Infant, Newborn; D010048:Ovarian Cysts / Q000150:complications* / Q000378:metabolism; D010053:Ovary / Q000201:enzymology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D006831:Polyhydramnios / Q000000981:diagnostic imaging; D011247:Pregnancy; D013250:Steroid Hydroxylases / Q000235:genetics / Q000378:metabolism*; D014463:Ultrasonography; D015854:Up-Regulation; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D013250:Steroid Hydroxylases