[Restriction analysis of streptomyces plasmid pSS 181].
Restriction analysis of plasmid was carried out with the use of 7 enzymes. Presence of restriction sites for endonucleases II EcoRI, Pstl, BamHI, SalGI, and BgII was established. A unique restriction site for endonuclease EcoRI on the plasmid pSS181 was found out, but endonucleases HindIII and XbaI had no restriction sites on the plasmid. The molecular size of this plasmid was 10.75 +/- 0.25 kb. As a result of hydrolysis of the plasmid DNAby pairs ofendonucleases (EcoRI+PstI) and (EcoRI+BamHI) it was established, that the site of restriction for enzyme EcoRI was located on the biggest PstI and BamHI-fragments.
Luk'ianchuk
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D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes