title: PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction.
abstract: This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4 degrees C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious.
authors: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors: Saiwasan
authors: S
authors: Buathong
authors: Mathirut
authors: M
authors: Mungthin
authors: Peerapan
authors: P
authors: Tan-ariya
authors: Tawee
authors: T
authors: Naaglor
authors: Paanjit
authors: P
authors: Taamasri
authors: Picha
authors: P
authors: Suwannahitatorn
authors: Saovanee
authors: S
authors: Leelayoova
mesh_terms: D000818:Animals; D003080:Cold Temperature; D018509:DNA, Helminth / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012965:Sodium Chloride; D013997:Time Factors
chemical_list: D018509:DNA, Helminth; D012965:Sodium Chloride