title: The effect of zinc on hydroxyapatite-mediated activation of human polymorphonuclear neutrophils and bone implant-associated acute inflammation.
abstract: Hydroxyapatite (HA) is widely used as coating biomaterial for prosthesis metal parts and as bone substitute. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. At the inflamed site, the polymorphonuclear cells (PMNs) represent the earliest phagocytic cells that predominate the cellular infiltrate. We have recently proposed that HA wear debris activate polymorphonuclear cells (PMNs) initiating and/or amplifying thereby the acute inflammatory response. Previous studies have shown that activation of monocytes by HA could be modulated by supplementing this latter with the divalent cation, Zinc. The purpose of this work was to investigate the modulation of PMNs activation following exposure to zinc-substituted HA. Our study demonstrate that addition of zinc to HA particles resulted in decreased levels of the pro-inflammatory mediator interleukin-8 (IL-8) and the matrix metallo-proteinase-9. We also show that these changes involve IL-8 receptors (CXCR-1 and CXCR-2).
authors: INSERM, UMR-S 926, CHU de Reims, IFR 53, URCA-1 avenue du Maréchal Juin, 51095 Reims, Cedex, France. frederic.velard@univ-reims.fr
authors: Frédéric
authors: F
authors: Velard
authors: Dominique
authors: D
authors: Laurent-Maquin
authors: Julien
authors: J
authors: Braux
authors: Christine
authors: C
authors: Guillaume
authors: Sylvie
authors: S
authors: Bouthors
authors: Edouard
authors: E
authors: Jallot
authors: Jean-Marie
authors: JM
authors: Nedelec
authors: Abderrazzaq
authors: A
authors: Belaaouaj
authors: Patrice
authors: P
authors: Laquerriere
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000276:immunology*; D002633:Chemotaxis / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry / Q000276:immunology*; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D008422:Materials Testing; D020780:Matrix Metalloproteinase 9 / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D023062:Receptors, Interleukin-8A / Q000276:immunology; D023063:Receptors, Interleukin-8B / Q000276:immunology; D015032:Zinc / Q000737:chemistry / Q000276:immunology*
chemical_list: D020099:Coated Materials, Biocompatible; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D017886:Durapatite; D020780:Matrix Metalloproteinase 9; D015032:Zinc