title: A periplasmic reducing system protects single cysteine residues from oxidation.
abstract: The thiol group of the amino acid cysteine can be modified to regulate protein activity. The Escherichia coli periplasm is an oxidizing environment in which most cysteine residues are involved in disulfide bonds. However, many periplasmic proteins contain single cysteine residues, which are vulnerable to oxidation to sulfenic acids and then irreversibly modified to sulfinic and sulfonic acids. We discovered that DsbG and DsbC, two thioredoxin-related proteins, control the global sulfenic acid content of the periplasm and protect single cysteine residues from oxidation. DsbG interacts with the YbiS protein and, along with DsbC, regulates oxidation of its catalytic cysteine residue. Thus, a potentially widespread mechanism controls sulfenic acid modification in the cellular environment.
authors: de Duve Institute, Université catholique de Louvain, B-1200 Brussels, Belgium.
authors: Matthieu
authors: M
authors: Depuydt
authors: Stephen E
authors: SE
authors: Leonard
authors: Didier
authors: D
authors: Vertommen
authors: Katleen
authors: K
authors: Denoncin
authors: Pierre
authors: P
authors: Morsomme
authors: Khadija
authors: K
authors: Wahni
authors: Joris
authors: J
authors: Messens
authors: Kate S
authors: KS
authors: Carroll
authors: Jean-François
authors: JF
authors: Collet
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism*; D033903:Periplasmic Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D013379:Substrate Specificity; D013434:Sulfenic Acids / Q000378:metabolism
chemical_list: D004220:Disulfides; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D013434:Sulfenic Acids; C545765:YbiS protein, E coli; D010088:Oxidoreductases; C109831:DsbG protein, E coli; D019704:Protein Disulfide-Isomerases; C526649:dsbC protein, E coli; D003545:Cysteine