[Effects of Sijunzi Decoction on urine's xylose excretion rate and ATP in mucosa of spleen deficiency rats].
OBJECTIVE
To observe the effects of Sijunzi Decoction on D-xylose excretion rate and ATP content in the mucosa membranes of small intestines of rats with spleen deficiency.


METHODS
Spleen deficiency model rats were made by reserpine injection. D-xylose excretion rate was measured with p-bromoaniline method, and the ATP content of small intestines mucosa was detected with bioluminescence method. The correlation between D-xylose excretion rate and ATP content of mucosa was also analyzed.


RESULTS
Rats' body weight and D-xylose excretion rate decreased after reserpine injection (P < 0.01, vs control group), but increased after treated with Sijunzi Decoction (P < 0.05, vs model group). The ATP content of mucosa showed no significant difference between model group and control group. There was obviously positive correlation between the change of urine's D-xylose excretion rate and mucosa ATP content.


CONCLUSIONS
Sijunzi Decoction has the activity of improving xylose absorption in spleen deficiency rats, but no obvious effect on their mucosa ATP content. The reducing of urine's D-xylose excretion rate in spleen deficiency rats is accompanied with the decrease of mucosa ATP content.
Gao
Xiao-Ling
XL
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Guo
Wen-Feng
WF
Li
Ru-Liu
RL
Chen
Wei-Wen
WW
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000008:administration & dosage; D013158:Splenic Diseases / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D014994:Xylose / Q000493:pharmacokinetics* / Q000652:urine
D004365:Drugs, Chinese Herbal; C403259:si-jun-zi-tang; D012110:Reserpine; D000255:Adenosine Triphosphate; D014994:Xylose