title: Proteomic analysis of the intestinal adaptation response reveals altered expression of fatty acid binding proteins following massive small bowel resection.
abstract: Intestinal adaptation in response to the loss of the small intestine is essential to restore enteral autonomy in patients who have undergone massive small bowel resection (MSBR). In a proportion of patients, intestinal function is not restored, resulting in chronic intestinal failure (IF). Early referral of such patients for transplant provides the best prognosis; however, the molecular mechanisms underlying intestinal adaptation remain elusive and there is currently no convenient marker to predict whether patients will develop IF. We have investigated the adaptation response in a well-characterized porcine model of intestinal adaptation. 2D DIGE analysis of ileal epithelium from piglets recovering from massive small bowel resection (MSBR) identified over 60 proteins that changed specifically in MSBR animals relative to nonoperational or sham-operated controls. Three fatty acid binding proteins (L-FABP, FABP-6, and I-FABP) showed changes in MSBR animals. The expression changes and localization of each FABP were validated by immunoblotting and immunohistochemical analysis. FABP expression changes in MSBR animals occurred concurrently with altered triglyceride and bile acid metabolism as well as weight gain. The observed FABP expression changes in the ileal epithelium occur as part of the intestinal adaptation response and could provide a clinically useful marker to evaluate adaptation following MSBR.
authors: Prince Henry's Institute of Medical Research, Monash Medical Centre, Clayton, Victoria, Australia. Andrew.stephens@princehenrys.org
authors: Andrew N
authors: AN
authors: Stephens
authors: Prue M
authors: PM
authors: Pereira-Fantini
authors: Guineva
authors: G
authors: Wilson
authors: Russell G
authors: RG
authors: Taylor
authors: Adam
authors: A
authors: Rainczuk
authors: Katie L
authors: KL
authors: Meehan
authors: Magdy
authors: M
authors: Sourial
authors: Peter J
authors: PJ
authors: Fuller
authors: Peter G
authors: PG
authors: Stanton
authors: David M
authors: DM
authors: Robertson
authors: Julie E
authors: JE
authors: Bines
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics / Q000502:physiology*; D000818:Animals; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D050556:Fatty Acid-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D007082:Ileum / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism / Q000502:physiology* / Q000601:surgery*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012778:Short Bowel Syndrome / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013552:Swine
chemical_list: D050556:Fatty Acid-Binding Proteins