Gummosis in grape hyacinth (Muscari armeniacum) bulbs: hormonal regulation and chemical composition of gums.
The purpose of this study was to investigate the hormonal regulation of gummosis in grape hyacinth (Muscari armeniacum) bulbs, focusing especially on the chemical composition of the gums. The application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at 1% and 2% (w/w) in lanolin as well as ethylene induced gummosis in the bulbs within several days. Methyl jasmonate (JA-Me, 0.1-2% in lanolin) alone had no effect on gummosis. However, simultaneous application of JA-Me and ethephon led to extreme stimulation of ethephon-induced gummosis. Ethephon-induced gummosis in the bulbs depended on the maturation stage of the bulbs, increasing from April to July, but decreasing from August to September. Regardless of the presence of JA-Me, the application of ethephon to the inflorescence axis of grape hyacinths did not induce gummosis. Gel permeation chromatography analysis revealed that gums were homogenous polysaccharides with an average molecular mass of ca. 8.3 kDa. Analysis of the sugar composition of the gums after hydrolysis revealed that the molar ratio of Rha:Ara:Gal:GalA:GlcA was 25:10:40:7:15. These results suggest that principal factors of gummosis as well as the chemical composition of gums differ between species of bulbous plants.
Miyamoto
Kensuke
K
Faculty of Liberal Arts and Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. miyamoto@las.osakafu-u.ac.jp
Kotake
Toshihisa
T
Sasamoto
Makiko
M
Saniewski
Marian
M
Ueda
Junichi
J
D000085:Acetates / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D031423:Hyacinthus / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D009943:Organophosphorus Compounds / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D053149:Plant Gums / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry* / Q000187:drug effects; D012621:Seasons; D027843:Vitis / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; D053149:Plant Gums; C072239:methyl jasmonate; C036216:ethylene; C005073:ethephon