Supercritical fluid extraction and high performance liquid chromatographic determination of benzopyrans and phloroglucinol derivative in Hypericum polyanthemum.
The aerial parts of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferae) were successively extracted with supercritical carbon dioxide (SC CO(2)) under pressures of 90, 120, 150 and 200 bar at different temperatures (40, 50 and 60 degrees C), and compared with the n-hexane extract obtained by ultrasound-assisted extraction. The samples obtained were examined regarding extraction yield and HPLC quantification of the main secondary metabolites, the benzopyrans HP1 (6-isobutyryl-5,7-dimethoxy-2,2-dimethylbenzopyran), HP2 (7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran) and HP3 (5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl) and the phloroglucinol derivative, uliginosin B. SFE presented higher selectivity than the n-hexane maceration, and the best condition to extract the target metabolites has been determined to be at 50 degrees C and for the high molecular-weight compound, uliginosin B, higher pressures were required.
Cargnin
Simone Tasca
ST
Programa de Pós Graduação em Ciências Farmacêuticas, UFRGS. Av. Ipiranga, 2752, 90610.000 Porto Alegre, RS, Brazil.
de Matos Nunes
Jéssica
J
Haas
Juliana Schulte
JS
Baladão
Luís Fernando
LF
Cassel
Eduardo
E
Vargas
Rubem Figueiró
RF
Rech
Sandra Beatriz
SB
von Poser
Gilsane Lino
GL
D000704:Analysis of Variance; D001578:Benzopyrans / Q000032:analysis* / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D006586:Hexanes / Q000737:chemistry; D020902:Hypericum / Q000737:chemistry*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D001578:Benzopyrans; D006586:Hexanes; D002245:Carbon Dioxide; C026385:n-hexane; D010696:Phloroglucinol; C547933:uliginosin B