[Screening of differentially expressed genes from patients with Bardet-Biedl syndrome by gene chip analysis].
OBJECTIVE
To investigate the differential gene expression profile from patients with Bardet-Biedl syndrome (BBS) by oligonucleotide microarray technique.


METHODS
Total RNA of 3 probands with BBS and 4 healthy siblings were isolated from peripheral blood mononuclear cells and reverse-transcribed to cDNAs. Then the cDNAs were subjected for microarray screening with Affymetrix U133 Plus 2.0 array. Genechip scanner was applied to screen the hybridization signals. Genes differentially expressed between the BBS probands and controls were identified by using GCOS1.4 software with the standard of two-fold change (P<0.05) of expression.


RESULTS
Fifteen genes were up-regulated 2 or more fold and another 15 genes were down-regulated 2 or more fold in the BBS patients, among them 12 genes were related to signaling pathway and cell cycle by Gene Ontology (GO) analysis.


CONCLUSIONS
The differentially expressed genes identified may correlate with the function or structure of cilia. Their roles in the BBS genesis need to be further studied.
Shen
Tao
T
Institute of Basic Medicine, the First People's Hospital of Yunnan Province, Kunhua Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, 650032 P R China.
Shou
Tao
T
Yan
Xinmin
X
Dong
Hong
H
Zou
Yunlian
Y
Gao
Jianmei
J
Zeng
Rong
R
Hua
Yingkun
Y
Zhang
Jinping
J
Li
Li
L
D000328:Adult; D020788:Bardet-Biedl Syndrome / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D035781:Siblings