title: Epigenome microarray platform for proteome-wide dissection of chromatin-signaling networks.
abstract: Knowledge of protein domains that function as the biological effectors for diverse post-translational modifications of histones is critical for understanding how nuclear and epigenetic programs are established. Indeed, mutations of chromatin effector domains found within several proteins are associated with multiple human pathologies, including cancer and immunodeficiency syndromes. To date, relatively few effector domains have been identified in comparison to the number of modifications present on histone and non-histone proteins. Here we describe the generation and application of human modified peptide microarrays as a platform for high-throughput discovery of chromatin effectors and for epitope-specificity analysis of antibodies commonly utilized in chromatin research. Screening with a library containing a majority of the Royal Family domains present in the human proteome led to the discovery of TDRD7, JMJ2C, and MPP8 as three new modified histone-binding proteins. Thus, we propose that peptide microarray methodologies are a powerful new tool for elucidating molecular interactions at chromatin.
authors: Department of Biology, Stanford University, Stanford, California, United States of America.
authors: Dennis J
authors: DJ
authors: Bua
authors: Alex J
authors: AJ
authors: Kuo
authors: Peggie
authors: P
authors: Cheung
authors: Chih Long
authors: CL
authors: Liu
authors: Valentina
authors: V
authors: Migliori
authors: Alexsandra
authors: A
authors: Espejo
authors: Fabio
authors: F
authors: Casadio
authors: Christian
authors: C
authors: Bassi
authors: Bruno
authors: B
authors: Amati
authors: Mark T
authors: MT
authors: Bedford
authors: Ernesto
authors: E
authors: Guccione
authors: Or
authors: O
authors: Gozani
mesh_terms: D000906:Antibodies / Q000032:analysis; D002843:Chromatin / Q000378:metabolism*; D044127:Epigenesis, Genetic; D015894:Genome, Human; D006657:Histones / Q000378:metabolism; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D020543:Proteome / Q000032:analysis*; D015398:Signal Transduction
chemical_list: D000906:Antibodies; D002843:Chromatin; D006657:Histones; D020543:Proteome