title: [Increase of saliva nitrate and nitrite level in patients with oral candidiasis].
abstract: OBJECTIVE
  To observe the alterations of saliva nitrate and nitrite level in patients with oral candidiasis.
  
  
  METHODS
  Parotid saliva and whole saliva were collected from 33 patients and 34 healthy volunteers. Concentrations of nitrate and nitrite in saliva were determined by high-performance liquid chromatography. Follow-up observation was performed on 10 patients after treatment. The data were statistically analyzed with independent-samples t test or paired-samples t test at alpha = 0.05.
  
  
  RESULTS
  There was significant increase of the concentrations and secretion rate of parotid saliva nitrate in patient group as compared with controls: (49.70 +/- 0.50) vs (21.51 +/- 0.60) mg/L (t = 2.692, P = 0.009) and (27.71 +/- 0.50) vs (12.55 +/- 0.60) microg/min (t = 2.554, P = 0.013), respectively. Significantly increased concentrations and secretion rate of nitrate and nitrite [nitrate: (6.46 +/- 0.94) vs (1.11 +/- 0.70) mg/L (t = 3.792, P = 0.000); nitrite: (8.48 +/- 0.58) vs (3.39 +/- 0.53) mg/L (t = 2.888, P = 0.005); nitrate secretion rate: (10.57 +/- 0.91) vs (2.10 +/- 0.74) microg/min (t = 3.464, P= 0.001); nitrite secretion rate: (13.91 +/- 0.55) vs (6.42 +/- 0.58) microg/min (t = 2.397, P = 0.020)] were revealed in whole saliva of patients group. Significantly decreased nitrate and nitrite levels were also observed in patients after treatment, especially the changes of parotid saliva nitrate secretion rate [(37.50 +/- 0.50) vs (14.34 +/- 0.64) microg/min (t = 3.142, P = 0.012)], whole saliva nitrate [(14.29 +/- 1.01) vs (2.59 +/- 1.03) mg/L (t = 3.475, P = 0.007)] and whole saliva nitrate secretion rate [(25.97 +/- 0.93) vs (4.12 +/- 1.00) microg/min (t = 3.922, P = 0.003)].
  
  
  CONCLUSIONS
  The present study revealed the significant increase of salivary nitrate and nitrite level in patients with oral candidiasis is considered to be associated with the host defense reaction.
authors: Salivary Gland Disease Center, Capital Medical University School of Stomatology, Beijing, China.
authors: Rui-Tang
authors: RT
authors: Shi
authors: Li-Zheng
authors: LZ
authors: Qin
authors: Deng-Sheng
authors: DS
authors: Xia
authors: Da-Jun
authors: DJ
authors: Deng
authors: Zhi-Peng
authors: ZP
authors: Fan
authors: Zhao-Chen
authors: ZC
authors: Shan
authors: Yan-Ying
authors: YY
authors: Xu
authors: Song-Ling
authors: SL
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D002180:Candidiasis, Oral / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*; D055815:Young Adult
chemical_list: D009566:Nitrates; D009573:Nitrites