title: Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast.
abstract: Faithful segregation of sister chromatids requires the attachment of each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt-MT misattachment in which a single Kt binds MTs from both spindle poles rather than just one. Genetic induction of merotelic Kt attachment during anaphase in fission yeast resulted in intra-Kt stretching followed by either correction or Kt disruption. Laser ablation of spindle MTs revealed that intra-Kt stretching and merotelic correction were dependent on MT forces. The presence of multiple merotelic chromosomes linearly antagonized the spindle elongation rate, and this phenomenon could be solved numerically using a simple force balance model. Based on the predictions of our mechanical model, we provide in vivo evidence that correction of merotelic attachment in anaphase is tension dependent and requires an Ase1/Prc1-dependent mechanism that prevents spindle collapse and thus asymmetric division and/or the appearance of the cut phenotype.
authors: Université de Toulouse, Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération UMR5088, F-31062 Toulouse, France.
authors: Thibault
authors: T
authors: Courtheoux
authors: Guillaume
authors: G
authors: Gay
authors: Yannick
authors: Y
authors: Gachet
authors: Sylvie
authors: S
authors: Tournier
mesh_terms: D000705:Anaphase; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000502:physiology; D008954:Models, Biological; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism / Q000502:physiology*
chemical_list: C502827:Ase1 protein, S pombe; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins