title: Glucosylglycerol and glucosylglycerate as enzyme stabilizers.
abstract: Compatible solutes constitute a diverse class of low-molecular-mass organic molecules that are accumulated in high intracellular concentrations in response to the external stress of hyperosmolality or high temperature. Many of these compounds like alpha, alpha-trehalose are well known for their stabilizing effect on protein structure and could lead to development of more stable protein formulations. Negatively charged solutes like mannosylglycerate (R-2-O-alpha-D-mannopyranosyl-glycerate) are widespread among (hyper)thermophilic microorganisms and are thought to be exceptionally potent stabilizers of proteins under high-temperature denaturation conditions. To further inquire into the role of compound charge for protective function, we have compared two naturally occurring and structurally related solutes, glucosylglycerol (2-O-alpha-D-glucopyranosyl-sn-glycerol) and glucosylglycerate (R-2-O-alpha-D-glucopyranosyl-glycerate), as stabilizers of different enzymes undergoing inactivation through elevated temperature or freeze drying, and benchmarked their effects against that of alpha,alpha-trehalose. Glucosylglycerate in concentrations of >/=0.1 M was the most effective in preventing thermally induced loss of enzyme activity of lactate dehydrogenase, mannitol dehydrogenase, starch phosphorylase, and xylose reductase. alpha,alpha-Trehalose could usually be replaced by glucosylglycerol without compromising enzyme stability. Glucosylglycerol and glucosylglycerate afforded substantial (eightfold) protection to mannitol dehydrogenase during freeze drying.
authors: Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/1, Graz, Austria.
authors: Thornthan
authors: T
authors: Sawangwan
authors: Christiane
authors: C
authors: Goedl
authors: Bernd
authors: B
authors: Nidetzky
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D005612:Freeze Drying; D005656:Fungal Proteins / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry*; D005988:Glyceric Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: C509251:2-glucosylglycerate; D001426:Bacterial Proteins; D004798:Enzymes; D005656:Fungal Proteins; D005960:Glucosides; D005988:Glyceric Acids; C034727:glucosylglycerol