title: State of homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma.
abstract: Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P<0.05). These results indicate that HOXA10 expression could contribute to cancer progression and prognosis and that HOXA10 may be a potential diagnostic marker and a therapeutic target for OSCCs.
authors: Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan.
authors: Masanobu
authors: M
authors: Yamatoji
authors: Atsushi
authors: A
authors: Kasamatsu
authors: Yukio
authors: Y
authors: Yamano
authors: Kentaro
authors: K
authors: Sakuma
authors: Kenji
authors: K
authors: Ogoshi
authors: Manabu
authors: M
authors: Iyoda
authors: Keiji
authors: K
authors: Shinozuka
authors: Katsunori
authors: K
authors: Ogawara
authors: Yuichi
authors: Y
authors: Takiguchi
authors: Masashi
authors: M
authors: Shiiba
authors: Hideki
authors: H
authors: Tanzawa
authors: Katsuhiro
authors: K
authors: Uzawa
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; C069634:HOXA10 protein, human