[Study on absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model].
OBJECTIVE
To study the absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model.


METHODS
The Caco-2 monolayer model was used to explore the effect of time and drug concentration on the absorption of puerarin and its nanoparticeles. The drug concentration was determined by high-performance liquid chromatography and the Papp was caculated.


RESULTS
The puerarin-PLGA-NP could significantly improve the absorption of puerarin. Puerarin was not only absorbed simply through passive diffusion, but also had relations with high dispersion of nanoparticles, increase of affinity and interaction with intestinal mucous membrane.


CONCLUSIONS
The main mechanism of puerarin and its nanoparticles across Caco-2 monolayer model is passive transference. Puerarin-NP could increase the absorption of the drug in intestinal epithlial.
Liu
Xi-Jing
XJ
Research Institute of Qinghua University in Shenzhen, Shenzhen 518057, China. lxj_1975@yahoo.com.cn
Jia
Qiang
Q
Wang
Cheng-Yuan
CY
Wang
Nai-Li
NL
D000042:Absorption; D001692:Biological Transport; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000493:pharmacokinetics*; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D011091:Polyesters; C033607:puerarin