[Effect of dendritic cells pulsed with Talpha146-162 on autoimmune myasthenia graves and the role of B cells activation].
OBJECTIVE
To explore the therapeutic effect of Talpha146-162-iMDCs on C57BL/6 mice with EAMG and the role of changes in B cell activation regulated by Cbl.


METHODS
Thirty four adult male C57BL/6 mice were randomly divided into EAMG group (A group), EAMG group with interventions (B group) and control group (C group). T-AChR antigens were injected to the mice in A and B groups to induce EAMG. Meanwhile, dendritic cells from immature bone marrows were cultured and pulsed with Talpha146-162, and injected to the mice in the intervention group. The mRNA expression of Cbl was detected by RT-PCR 90 days after the termination of experiment. The expression and phosphorylation of Syk and Lyn proteins were measured by Western blot.


RESULTS
More mice (9/12, 75%) in A group developed EAMG than in B group (3/12, 25%, P<0.05). Mice in A group also suffered more serious illness in terms of clinical scores than mice in B group (1.69+/-1.12 vs 0.35+/-0.67, P<0.01). No EAMG occurred in mice in C group. Mice in B group had lower mRNA expressions of Cbl in the spleens and lymphonodes than mice in A groups, but both had higher expressions than mice in C group (P<0.05). Mice in B group had higher mRNA expression of Cbl than mice in A group (P<0.05).


CONCLUSIONS
Talpha146-162-iMDCs prevent EAMG, possibly through negative regulation of Cbl on BCR signaling.
Wang
Rong
R
Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.
Yang
Huan
H
Xiao
Bo
B
Zhang
Li-Fang
LF
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D011950:Receptors, Cholinergic / Q000276:immunology*; D000072377:Syk Kinase; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D050721:Proto-Oncogene Proteins c-cbl; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; C493849:Cbl protein, mouse