Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2).
Overexpression of antisense chromosomal cis-encoded noncoding RNAss (ncRNAs) in glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including several ncRNAs that are differentially expressed in bacterial hormone-defective mutants.
D'Alia
Davide
D
Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
Nieselt
Kay
K
Steigele
Stephan
S
Müller
Jonas
J
Verburg
Ilse
I
Takano
Eriko
E
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015153:Blotting, Western; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000502:physiology*
D000900:Anti-Bacterial Agents; D016372:RNA, Antisense; D012329:RNA, Bacterial; D022661:RNA, Untranslated; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase