title: Identification of avermectin-high-producing strains by high-throughput screening methods.
abstract: Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
authors: Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.
authors: Hong
authors: H
authors: Gao
authors: Mei
authors: M
authors: Liu
authors: Xianlong
authors: X
authors: Zhou
authors: Jintao
authors: J
authors: Liu
authors: Ying
authors: Y
authors: Zhuo
authors: Zhongxuan
authors: Z
authors: Gou
authors: Bing
authors: B
authors: Xu
authors: Wenquan
authors: W
authors: Zhang
authors: Xiangyang
authors: X
authors: Liu
authors: Aiqun
authors: A
authors: Luo
authors: Chuansen
authors: C
authors: Zheng
authors: Xiaoping
authors: X
authors: Chen
authors: Lixin
authors: L
authors: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D057166:High-Throughput Screening Assays; D007218:Industrial Microbiology; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D015203:Reproducibility of Results; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D007559:Ivermectin; C019264:avermectin