title: [Regulatory effects of streptococcal protein on the immunologic activity of RAW264.7 murine macrophages.].
abstract: OBJECTIVE
  To investigate the regulatory effects and the related mechanisms of streptococcal protein on the immunologic activity of RAW264.7 murine macrophages.
  
  
  METHODS
  RAW264.7 cells were incubated with different concentrated streptococcal protein respectively. The proliferation of RAW264.7 cells was determined by MTT assay. The phagocytosis of macrophages was analyzed by neutral red phagocytosis assay. The activities of IL-6 and TNF-alpha in the cultured supernatants were measured by bio-chemical methods. The mRNA expression of TNF-alpha, IL-6 and Toll-like receptors (TLRs) in cells was detected by reverse transcription polymerase chain reaction (RT-PCR). The expression of TLR2 and TLR4 in peritoneal cells was measured by flow cytometry.
  
  
  RESULTS
  Streptococcal protein significantly enhanced the activities of proliferation and phagocytosis of macrophages (P<0.05), induced the release of IL-6, TNF-alpha and the expression of IL-6, TNF-alpha mRNA (P<0.05).The streptococcal protein, meanwhile, also up-regulated the expression of TLR2 and TLR4 in macrophages in vitro (P<0.05).
  
  
  CONCLUSIONS
  Streptococcal protein may promote proliferation, phagocytosis and cytokines production in regulation of the activity of murine macrophages.
authors: Department of Pathogenic Biology, Capital Medical University, Beijing 100069, China.
authors: Yan
authors: Y
authors: Wang
authors: Qin
authors: Q
authors: Li
authors: Yi-Song
authors: YS
authors: Wang
authors: Hui
authors: H
authors: Chen
authors: Qun
authors: Q
authors: Zheng
authors: Guo-Ling
authors: GL
authors: Ping
authors: Li-Ping
authors: LP
authors: Zhang
mesh_terms: D000818:Animals; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha