title: [Effect of ginseng-sanqi extract on the Ras associated signal proteins].
abstract: OBJECTIVE
  To explore the effect of ginseng-sanqi extract (GSE) on the vascular endothelium growth factor receptor-2 (VEGFR-2), Ras and mitogen-activated protein kinase (MAPK) in VEGFR-2-Ras-MAPK signal pathway of angiogenesis in cultured human umbilical vascular endothelial cells (HUVECs).
  
  
  METHODS
  The protein expressions of VEGFR-2, Ras and MAPK in HUVEC and the effects of GSE (at different doses) and basic fibrin growth factor (bFGF) on them were detected by Western-Blot test.
  
  
  RESULTS
  GSE can enhance the expression of angiogenesis signaling proteins (VEGFR-2, Ras, MAPK) to different extents, especially in the groups treated by bFGF or higher dose GSE, the levels showed significant differences to those in the blank group (P<0.05).
  
  
  CONCLUSIONS
  GSE can promote the expressions of VEGFR-2, Ras and MAPK in the angiogenesis signaling pathway, which may be the foundation of Chinese medicine for tonifying qi and activating blood circulation in promoting endothelial proliferation and angiogenesis.
authors: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
authors: Wei
authors: W
authors: Tian
authors: Yan
authors: Y
authors: Lei
authors: Ling-Qun
authors: LQ
authors: Zhu
mesh_terms: D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D040301:Vascular Endothelial Growth Factor Receptor-2; D020928:Mitogen-Activated Protein Kinases