Physicochemical properties of transferrin-associated lipopolyplexes and their role in biological activity.
The combination of polyethylenimine (PEI), as a plasmid DNA pre-condensing agent, and cationic lipids has been reported to result in a synergistic effect on transfection. Recently, we have explored this effect by associating low-molecular weight PEIs with transferrin-associated lipoplexes using different cationic liposome formulations. The resulting lipopolyplexes that have shown to be the most efficient in mediating transfection were those prepared from cationic liposomes composed of DOTAP:Chol (associated or not with transferrin) and from a pH-sensitive liposome formulation (DOTAP:Chol:DOPE:CHEMS). In the present work, the physicochemical properties of these lipopolyplexes were studied aiming at establishing a correlation with their transfection efficiency. For this purpose, the lipopolyplexes were characterized in terms of their morphology by performing ultrastructural studies using cryo-TEM microscopy, investigating inner DNA structure using circular dichroism and characterizing particle size by photon correlation spectroscopy. A correlation between efficiency of transfection and more compact inner DNA structure and smaller particle sizes (around 250nm) was found. In addition, the visualization of liposomes and lipopolyplexes at the ultrastructural level revealed that the particles presenting enhanced transfection efficiencies are associated with higher electron density. Recently, PEI-based lipopolyplexes were reported to gain entry into the cell through the caveolae-mediated pathway. Based on the present finding that DOTAP:Chol liposomes exhibit the ability to form hexagonal structures when prepared at high concentrations, we propose that the lipopolyplexes containing DOTAP:Chol take advantage of such capacity to escape from the endocytotic vesicles, which will contribute to the observed high transfection efficiencies.
Penacho
Nuno
N
Department of Vectors and Gene Therapy, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Rosa
Mónica
M
Lindman
Björn
B
Miguel
Maria G
MG
Simões
Sérgio
S
de Lima
Maria C Pedroso
MC
D055598:Chemical Phenomena; D002942:Circular Dichroism; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection; D014168:Transferrin / Q000737:chemistry* / Q000502:physiology
D008081:Liposomes; D014168:Transferrin; D011094:Polyethyleneimine