Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei.
Human embryonic stem (hES) cell lines have been derived from normally or abnormally fertilized zygotes. However, the similar and different properties of these two types of hES cell lines are not well-known. To address this question, we generated nine hES cell lines from zygotes containing normal (2PN) and abnormal (0PN, 1PN, 3PN) pronuclei. A side-by-side comparison showed that all cell lines exhibited distinct identity and karyotypical stability. They expressed similar "stemness" markers and alkaline phosphatase activity and differentiated into three embryonic germ lineages in embryoid bodies and teratomas. Under neural differentiation-promoting conditions, they were directed into neural progenitors and neurons. However, a variation in cell cycle and the relative abundance of gene expression of undifferentiated and differentiated markers were observed. These variations were also seen among individually derived normal hES cell lines. Thus, normal hES cell lines can be developed from fertilized zygotes with abnormal pronuclei usually excluded from clinical use.
Huan
Qing
Q
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
Gao
Xuan
X
Wang
Yan
Y
Shen
Yun
Y
Ma
Wu
W
Chen
Zi-Jiang
ZJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D016172:DNA Fingerprinting; D053595:Embryonic Stem Cells / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D018895:Microsatellite Repeats; D019042:Muscle Neoplasms / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013724:Teratoma / Q000473:pathology; D015053:Zygote / Q000201:enzymology / Q000473:pathology*
D015415:Biomarkers; D000469:Alkaline Phosphatase