Identification and expression analysis of ras gene in silkworm, Bombyx mori.
Ras proteins play important roles in development especially for cell proliferation and differentiation in various organisms. However, their functions in the most insect species are still not clear. We identified three ras cDNAs from the silk worm, Bombyx mori. These sequences corresponded to three Ras of Drosophila melanogaster, but not to three mammalian Ras (H-Ras, K-Ras, N-Ras). Subsequently, the expression profiles of ras were investigated by quantitative real-time PCR using whole body of individuals from the embryonic to adult stages, and various tissues of 4th and 5th instar larvae. Each of three Bombyx ras showed different expression patterns. We also showed membrane localization of their products. These results indicate that the three Bombyx Ras are functional and have different roles.
Ogura
Takehiko
T
Department of Applied Life Sciences, Kyoto University, Kyoto, Japan.
Tan
Anjiang
A
Tsubota
Takuya
T
Nakakura
Takayo
T
Shiotsuki
Takahiro
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx; D003001:Cloning, Molecular; D017931:DNA Primers; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D011905:Genes, ras; D049452:Green Fluorescent Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018631:ras Proteins / Q000378:metabolism*
D017931:DNA Primers; D049452:Green Fluorescent Proteins; D018631:ras Proteins