title: Peripheral B cell abnormalities in patients with systemic lupus erythematosus in quiescent phase: decreased memory B cells and membrane CD19 expression.
abstract: B lymphocytes from patients with systemic lupus erythematosus (SLE) are hyperactive and produce autoantibodies. Several B cell phenotype characteristics such as the expansion of activated populations, and of a newly identified memory compartment have already been reported. These results are not easy to interpret because of the clinical heterogeneity of SLE, as well as the difficulties to establish homogeneous and well defined groups taking in consideration the activity of the disease and the various therapies. However, although many mediators and mechanisms can contribute to the clinical presentation and subsequent progression of individuals with SLE, several data suggest that some intrinsic B cells abnormalities may be central to the disease process. In this view, we have analysed the phenotype of B cells from 18 patients with quiescent diseases (mean SLEDAI score below 2) and from 11 healthy controls. B cell surface marker expression was determined by flow cytometry. We analysed the main B cell sub-populations. We demonstrate the persistence of plasmocyte-differentiated and -activated B cells even in quiescent patients. However, quiescent patients display a decrease in memory B cells that could reflect the control of their disease. Above all, we describe a lower membrane expression of the CD19 protein on all B cells in every patient compared to controls. This lower CD19 expression is associated with reduced CD45 levels. It is not associated with an evident gene expression alteration and in vitro stimulation restores a control phenotype. These findings suggest certain mechanisms of lupus development.
authors: CNRS UPR9021, Université de Strasbourg (UDS), Hopitaux Universitaires de Strasbourg, Strasbourg, France. Anne-Sophie.Korganow@hemato-ulp.u-strasbg.fr <Anne-Sophie.Korganow@hemato-ulp.u-strasbg.fr>
authors: Anne-Sophie
authors: AS
authors: Korganow
authors: Anne-Marie
authors: AM
authors: Knapp
authors: Hélène
authors: H
authors: Nehme-Schuster
authors: Pauline
authors: P
authors: Soulas-Sprauel
authors: Vincent
authors: V
authors: Poindron
authors: Jean-Louis
authors: JL
authors: Pasquali
authors: Thierry
authors: T
authors: Martin
mesh_terms: D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology*; D016022:Case-Control Studies; D015536:Down-Regulation; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008565:Membrane Proteins; D008875:Middle Aged; D055815:Young Adult
chemical_list: D018941:Antigens, CD19; D008565:Membrane Proteins; D017493:Leukocyte Common Antigens