title: Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.
abstract: Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance.
authors: HIV Drug Resistance Program, NCI, NIH, Frederick, MD, United States.
authors: Valerie F
authors: VF
authors: Boltz
authors: Frank
authors: F
authors: Maldarelli
authors: Neil
authors: N
authors: Martinson
authors: Lynn
authors: L
authors: Morris
authors: James A
authors: JA
authors: McIntyre
authors: Glenda
authors: G
authors: Gray
authors: Mark J
authors: MJ
authors: Hopley
authors: Toshio
authors: T
authors: Kimura
authors: Douglas L
authors: DL
authors: Mayers
authors: Patrick
authors: P
authors: Robinson
authors: John W
authors: JW
authors: Mellors
authors: John M
authors: JM
authors: Coffin
authors: Sarah E
authors: SE
authors: Palmer
mesh_terms: D000483:Alleles; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral