Insights to transcriptional networks by using high throughput RNAi strategies.
RNA interference (RNAi) is a powerful method to unravel the role of a given gene in eukaryotic cells. The development of high throughput assay platforms such as fluorescence plate readers and high throughput microscopy has allowed the design of genome wide RNAi screens to systemically discern members of regulatory networks around various cellular processes. Here we summarize the different strategies employed in RNAi screens to reveal regulators of transcriptional networks. We focus our discussion in experimental approaches designed to uncover regulatory interactions modulating transcription factor activity.
Mattila
Jaakko
J
Merck Research Laboratories, Department of Molecular Profiling-Research Informatics, Rahway, NJ 07065, USA. oscar_puig@merck.com
Puig
Oscar
O
D000818:Animals; D053263:Gene Regulatory Networks / Q000235:genetics*; D017930:Genes, Reporter / Q000502:physiology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D034622:RNA Interference / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
D014157:Transcription Factors