title: Identification of actinomycetes producing phospholipase d with high transphosphatidylation activity.
abstract: Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA-DNA hybridization tests indicated taxonomic affiliations of strain 9-6 with Streptomyces senoensis, strains 10-1 and 10-6 with S. vinaceus, and strains 10-2 and 10-3 with S. racemochromogenes. Strain 21-4, though identified as a Streptomyces sp., could not be identified with any known species. Meanwhile, most of the culture supernatants of reference strains demonstrated no or very weak PLD activity, while those of our strains exhibited significantly higher activity. All of the strains in this study were identified as Streptomyces species. The PLD activity of our strains exceeded most of the reference Streptomyces strains. The findings in this study imply that the Streptomyces strains, although they are members of the same species, can produce different quantities of PLD enzyme.
authors: Department of Food Science and Technology, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan. y3nakaza@bioindustry.nodai.ac.jp
authors: Yozo
authors: Y
authors: Nakazawa
authors: Rei
authors: R
authors: Suzuki
authors: Masataka
authors: M
authors: Uchino
authors: Yoshimasa
authors: Y
authors: Sagane
authors: Takuji
authors: T
authors: Kudo
authors: Takeshi
authors: T
authors: Nagai
authors: Hiroaki
authors: H
authors: Sato
authors: Katsumi
authors: K
authors: Takano
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010739:Phospholipase D / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D010739:Phospholipase D