title: [The influences of Tet system on activity and tissue specificity of HBV Cp.].
abstract: OBJECTIVE
  Analyze the effect of Tetracycline-controlled system on Hepatitis B virus core promoter (Cp) activity and tissue specificity.
  
  
  METHODS
  The 7 Teto sequence was amplified from plasmid pTL-8 using polymerase chain reaction (PCR) and cloned into T vector pMD19-T Simple. After sequencing, the 7 teto sequence was subcloned into upstream of Cp in pGL3-Basic/Cp. In order to observe the effect of Tetracycline-controlled system on Cp activity and tissue specificity, the plasmid pTL-8 which luciferase encoding sequence was excised and pGL3-Basic/7t/Cp were cotransfected into different tissue-derived cell lines including HepG2, Hela, COS-7, MDA-MB-231 and HT-29. The dual luciferase activities were determined by Dual Luciferase Report (DLR) assay system.
  
  
  RESULTS
  Have successfully constructed plasmid pGL3-Basic/7t/Cp. The transcriptional activity of Cp was increased significantly after the teto sequence was cloned upstream of Cp.
  
  
  CONCLUSIONS
  The transcriptional activity of Cp is augmented significantly by Tetracycline-controlled system. But the tissue specificity of Cp lost at the same time.
authors: Department of Etiology, School of Basic Medicine, Xi'an 710032, China.
authors: Jun-Chuan
authors: JC
authors: Lei
authors: Wei-Dong
authors: WD
authors: Gong
authors: Ya
authors: Y
authors: Zhao
authors: He
authors: H
authors: Jiang
authors: Zhong-Xiang
authors: ZX
authors: Liu
authors: Jun
authors: J
authors: Yi
mesh_terms: D005822:Genetic Vectors; D006515:Hepatitis B virus / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009928:Organ Specificity; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D008156:Luciferases