title: Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart.
abstract: BACKGROUND
  The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice.
  
  
  RESULTS
  We generated transgenic mice expressing mCherry-LC3 under alphaMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation.
  
  
  CONCLUSIONS
  This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206).
authors: Department of Cardiovascular Medicine, Akita University Graduate School of Medicine, Japan.
authors: Mai
authors: M
authors: Terada
authors: Kiyoshi
authors: K
authors: Nobori
authors: Yoshiko
authors: Y
authors: Munehisa
authors: Manabu
authors: M
authors: Kakizaki
authors: Takayoshi
authors: T
authors: Ohba
authors: Yoichiro
authors: Y
authors: Takahashi
authors: Takashi
authors: T
authors: Koyama
authors: Yutaka
authors: Y
authors: Terata
authors: Masaru
authors: M
authors: Ishida
authors: Kenji
authors: K
authors: Iino
authors: Toshimitsu
authors: T
authors: Kosaka
authors: Hiroyuki
authors: H
authors: Watanabe
authors: Hitoshi
authors: H
authors: Hasegawa
authors: Hiroshi
authors: H
authors: Ito
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy / Q000502:physiology*; D009202:Cardiomyopathies / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*
chemical_list: C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D049452:Green Fluorescent Proteins; D018995:Myosin Heavy Chains