title: Genome-wide proximal promoter analysis and interpretation.
abstract: High-throughput gene expression technologies based on DNA microarrays allow the examination of biological systems. However, the interpretation of the complex molecular descriptions generated by these approaches is still challenging. The development of new methodologies to identify common regulatory mechanisms involved in the control of the expression of a set of co-expressed genes might enhance our capacity to extract functional information from genomic data sets. In this chapter, we describe a method that integrates different sources of information: gene expression data, genome sequence information, described transcription factor binding sites (TFBSs), functional information, and bibliographic data. The starting point of the analysis is the extraction of promoter sequences from a whole genome and the detection of TFBSs in each gene promoter. This information allows the identification of enriched TFBSs in the proximal promoter of differentially expressed genes. The functional and bibliographic interpretation of the results improves our biological insight into the regulatory mechanisms involved in a microarray experiment.
authors: CEIT, Centro de Estudios e Investigaciones Técnicas de Gipuzkoa, San Sebastian, Spain.
authors: Elizabeth
authors: E
authors: Guruceaga
authors: Victor
authors: V
authors: Segura
authors: Fernando J
authors: FJ
authors: Corrales
authors: Angel
authors: A
authors: Rubio
mesh_terms: D001665:Binding Sites; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods*; D003627:Data Interpretation, Statistical; D030541:Databases, Genetic; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics; D007371:Interferon-gamma / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D014157:Transcription Factors
chemical_list: D016898:Interferon-alpha; D014157:Transcription Factors; D007371:Interferon-gamma