Expression of P311, a transforming growth factor beta latency-associated protein-binding protein, in human kidneys with IgA nephropathy.
BACKGROUND
In cultured NIH3T3 cells, P311 binds to the transforming growth factor-beta (TGF-β)-1 latency-associated protein (LAP) and induces the myofibroblast phenotype. In this study, we determined the levels of P311 and TGF-β1 proteins in tubulointerstitial tissue of patients with different severities of immunoglobulin-A nephropathy (IgAN), and analyzed the relationships between P311 protein expression and clinical data.


METHODS
A total of 57 patients with IgAN and 5 controls (from partial nephrectomy) were included. P311 and TGF-β1 protein expression were measured by immunohistochemistry and clinical data (proteinuria, serum creatinine (Scr), eGFR and biopsy index) were recorded. The relationship between P311, TGF-β1, and clinical data was analyzed.


RESULTS
P311 expression was significantly higher in the kidneys of IgAN patients than in controls and was higher in patients with advanced pathological grades of IgAN. P311 protein expression in tubulointerstitial tissue correlated with TGF-β1 and proteinuria. P311 expression was higher in patients with Scr > 133 μmol/L than in patients with Scr < 133 μmol/L.


CONCLUSIONS
P311 protein expression in the kidneys of IgAN patients correlates with TGF-β1 expression and with proteinuria. P311 might be a key cytokine in renal fibrosis and be involved in the progression of IgAN.
Wang
Fengping
F
Department of Nephrology, Sichuan University West China Hospital, No. 37 Guoxue Xiang, 610041 Wu Hou District, Chengdu, Sichuan Province, China. wfp2003@tom.com
Xie
Xisheng
X
Fan
Junming
J
Wang
Li
L
Guo
Dongyang
D
Yang
Lichuan
L
Ma
Xiaofen
X
Zhang
Lina
L
Li
Zi
Z
D000328:Adult; D005260:Female; D005922:Glomerulonephritis, IGA / Q000378:metabolism* / Q000652:urine; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D011507:Proteinuria; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
C428027:NREP protein, human; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D053773:Transforming Growth Factor beta1