Isolation and screening of microorganisms for R-(+)-limonene and (-)-beta-pinene biotransformation.
This work is focused on the biotransformation of R-(+)-limonene and (-)-beta-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for limonene degradation. At the screening step, eight strains were able to convert R-(+)-limonene and 15 to transform (-)-beta-pinene, both in alpha-terpineol. The highest concentration in alpha-terpineol from R-(+)-limonene was about 3,450 mg/L for Penicillium sp. isolated from eucalyptus steam. From (-)-beta-pinene, the highest product concentration of 675.5 mg/L was achieved using an Aspergillus sp. strain isolated from orange tree stem.
Rottava
Ieda
I
Department of Biochemistry, Instituto de Química-UFRJ, CT, Bloco A, Lab 641, Rio de Janeiro, Rio de Janeiro 21945-970, Brazil.
Cortina
Priscila F
PF
Grando
Camila E
CE
Colla
André R S
AR
Martello
Eduarda
E
Cansian
Rogério L
RL
Toniazzo
Geciane
G
Treichel
Helen
H
Antunes
Octávio A C
OA
Oestreicher
Enrique G
EG
de Oliveira
Débora
D
D001230:Aspergillus / Q000378:metabolism; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000378:metabolism*; D000077222:Limonene; D039821:Monoterpenes / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C016775:alpha-terpineol; C010789:beta-pinene; D000077222:Limonene