Down-regulated expression of cytochrome P450 (CYP) involved in the development and progression of esophageal adenocarcinoma.
OBJECTIVE
To analyze the differential expression genes (DEGs) between esophageal adenocarcinoma (EAC) and para-cancerous tissue (PCT) and explore the target genes related to the development and progression of EAC.


METHODS
The total RNAs of matched EAC and para-cancerous tissue of EAC patients were isolated using one step Trizol method. Matched RNAs were qualified using 10 g/L agarose gel electrophoresis. cRNAs were synthesized, fluorescence labeled and purified after total RNAs were purified. The RNAs of EAC and PCT were hybridized with Agilent oligomicroarray (30,968 probes). The fluorescence intensity features were detected by Agilent scanner and quantified by feature extraction software.


RESULTS
(1)The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality; (2)There were 212 up-regulated genes and 126 down-regulated genes am- ong 2-fold DEGs, including 16 genes related to cytochrome P450 (CYP).


CONCLUSIONS
Many EAC-assoeiated genes were screened by the high-throughput gene chip method. The development and progression of EAC is a complicated process involving multigenes and multiprocedures. The down-regulated expression of CYP related genes and gene polymorphism of CYP2 subfamily may be involved in the onset and progress of EAC.
Wang
Xing-Wei
XW
Gastroenterology Research Institute, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Xu
Mei
M
Wang
Wei-Qiang
WQ
Wang
Hong-Bin
HB
Sun
Yong-Gang
YG
Zhou
Gang
G
Gao
Heng-Jun
HJ
Fang
Dian-Chun
DC
D000230:Adenocarcinoma / Q000201:enzymology* / Q000209:etiology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000209:etiology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System