The post-mortem relationship between beta-hydroxybutyrate (BHB), acetone and ethanol in ketoacidosis.
A reduced blood pH (ketoacidosis) from the production of beta-oxidative ketone bodies as a result of alcoholism (alcoholic ketoacidosis, AKA) or diabetes (diabetic ketoacidosis, DKA) can feature in many fatalities and analytical evidence can be used to support a pathological diagnosis, or provide a possible cause of death in the absence of other pathologically significant findings. Existing beliefs concerning the relationship of BHB concentrations, acetone and ethanol have been re-examined by analysis of BHB, acetone and ethanol in over 350 fatalities grouped into alcoholics, diabetics, alcoholic diabetics, coupled with speculative cases and those with an alternative cause of death. Uniquely, the concentrations of BHB were measured in post-mortem blood, urine and vitreous humour using selective GC-MS. The results showed that existing beliefs need to be re-evaluated. Ethanol is not always low (<10mg/dL) or absent in cases of AKA. Also, the absence of acetone precludes [corrected] a high BHB (>250mg/L), therefore acetone can be used as an initial marker pathologically significant ketoacidosis. For blood and urine BHB concentrations the following interpretative ranges can be used (in mg/L); normal (<50mg/L), raised (51-249mg/L), high and pathologically significant (>250mg/L). Initial data suggest vitreous humour BHB could be a useful alternative in the absence of blood (same interpretative ranges may also apply). Analytical recommendation for investigation of post-mortem ketoacidosis is also presented.
Elliott
Simon
S
ROAR Forensics, Malvern Hills Science Park, Malvern, UK. simontox@yahoo.co.uk
Smith
Christopher
C
Cassidy
Diane
D
D020155:3-Hydroxybutyric Acid / Q000032:analysis*; D000096:Acetone / Q000032:analysis*; D000138:Acidosis / Q000175:diagnosis*; D000428:Alcohol Drinking / Q000009:adverse effects; D015415:Biomarkers; D002492:Central Nervous System Depressants / Q000032:analysis*; D016883:Diabetic Ketoacidosis / Q000175:diagnosis*; D000431:Ethanol / Q000032:analysis*; D005410:Flame Ionization; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D014822:Vitreous Body / Q000737:chemistry
D015415:Biomarkers; D002492:Central Nervous System Depressants; D000096:Acetone; D000431:Ethanol; D020155:3-Hydroxybutyric Acid