title: Synchrony of G6PD activity and RBC fragility under oxidative stress exerted at normal and G6PD deficiency.
abstract: OBJECTIVE
  To investigative the effects of oxidative stress simultaneously on Glucose-6-phosphate dehydrogenase (G6PD) activity and RBC fragility under normal and G6PD defective conditions.
  
  
  METHODS
  The effects of several nitric oxide (NO) generating compounds and sulfhydryl blocking agents were simultaneously tested in vitro on hemolysate G6PD activities and RBC fragility. These effects were compared between normal subjects and patients with G6PD deficiency.
  
  
  RESULTS
  The NO donor compounds nitrosocysteine, nitrosoarginine and diethylamine caused strong inhibition on normal and defective G6PD activities, while a similar inhibition was observed only at higher concentrations of the sulfhydryl blocking agents: 2-mercaptoethanol , cysteine and reduced glutathione. All these oxidative compounds promoted RBC hemolysis in parallel to their inhibition extents on G6PD activities. The protection of RBC from this hemolysis was achieved by preincubation with NADPH or SNP but not NAD(+) compound.
  
  
  CONCLUSIONS
  A concomitant response of G6PD activities and RBC fragility towards the oxidative stress was established.
authors: Pharmacology Department, Faculty of Medicine, Mutah University, Al-Karak, 61710, Jordan. muayadabboud@yahoo.com
authors: Muayad M
authors: MM
authors: Abboud
authors: Wajdy
authors: W
authors: Al-Awaida
mesh_terms: D019610:Cytoprotection / Q000187:drug effects; D004048:Diethylamines / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000201:enzymology* / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D004048:Diethylamines; D020030:Nitric Oxide Donors; D013438:Sulfhydryl Compounds; D009599:Nitroprusside; C034281:diethylamine; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005978:Glutathione