title: Deficiency of pulmonary Valpha24 Vbeta11 natural killer T cells in corticosteroid-naïve sarcoidosis patients.
abstract: Invariant natural killer T (NKT) cells might contribute to the amplified and prolonged T-cell immune response that characterizes sarcoidosis. Therefore, we want to investigate the frequency and distribution of pulmonary invariant NKT cells in corticosteroid-naïve patients with sarcoidosis. We used multi-parameter flow cytometry with antibodies against CD3, CD4, CD8, CD14, CD19, CD45, CD16/56, TCR Valpha24, and TCR Vbeta11, on bronchoalveolar lavage fluid (BALF), to examine the frequency and distribution of pulmonary invariant NKT cells in 47 newly diagnosed sarcoidosis patients and in 8 control subjects. The frequencies of BALF Valpha24 Vbeta11 invariant NKT cells were significantly lower in patients with sarcoidosis in comparison to control subjects. Moreover, lower invariant NKT cell frequencies in patients with sarcoidosis significantly correlated with exaggerated BALF lymphocytosis and CD4 T cell responses. This study demonstrated a pulmonary deficiency in the frequency of a subset of T cells with immunoregulatory function in patients with sarcoidosis.
authors: University Clinic of Respiratory and Allergic Diseases, Golnik 36, 4204 Golnik, Slovenia. peter.korosec@klinika-golnik.si
authors: Peter
authors: P
authors: Korosec
authors: Matija
authors: M
authors: Rijavec
authors: Mira
authors: M
authors: Silar
authors: Izidor
authors: I
authors: Kern
authors: Mitja
authors: M
authors: Kosnik
authors: Katarina
authors: K
authors: Osolnik
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000276:immunology; D016516:CD4-CD8 Ratio; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D017565:Sarcoidosis, Pulmonary / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell; C528689:Valpha24 protein, human