[Analysis of antibody profiles against retinal antigens in serum from patients with glaucoma through mass spectrometry].
OBJECTIVE
To compare the difference of the antibody profile between the primary open angle glaucoma (POAG) and the healthy controls (CTRL).


METHODS
It was a case-control study. Sera from 44 POAG and 48 CTRL were added to tubes containing pre-washed Protein G beads, which can capture IgGs. These IgG-Protein G-complexes were used to capture target antigens (homogenized bovine retina). After elution, the antigenic proteins were measured through surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. By t-test and multivariate statistical analysis, the spectra, the age and the gender between the two groups were compared; subsequently, the antigenic mass spectra data combined with the type of protein chips and the energy of laser desorption/ionization between these two groups were also compared, which indirectly reflect the antibody profiles of these two groups.


RESULTS
The mean intensity of spectra were 4244.427 +/- 4721.115 in CTRL and 4049.864 +/- 4083.819 in POAG, which showed statistical difference (F = 1.340, P < 0.01); complex antibody profiles were found in both groups. Significantly different immunoreactive spectra were detected between POAG patients and control subjects. Some up-, but mainly down-regulated immunoreactivities were present in POAG patients.


CONCLUSIONS
A significant different pattern of antibody profile is present between the POAG and CTRL. Down-regulated immunoreactivities present in POAG might mean an association between the "aberrant autoimmunity" and the primary open angle glaucoma.
Yan
Liang
L
Baoshan Central Hospital, Shanghai 201900, China. yliang670126@126.com
Joachim
S C
SC
Pfeiffer
N
N
Grus
H F
HF
Erb
C
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G