title: Bifunctional combined aptamer for simultaneous separation and detection of thrombin.
abstract: Here we report on the construction and evaluation of a bifunctional combined aptamer (BCA) that consists of a DNA streptavidin-binding aptamer (SBA), a DNA thrombin-binding aptamer (TBA) and a fluorophore. The BCA adopts a new conformation that is very different from simply linking the conformations of the two individual aptamers together, so that it does not bind to streptavidin in the absence of thrombin. Binding of this novel DNA aptamer to streptavidin is triggered by the thrombin binding and depends on the concentration of thrombin. Meanwhile, fluorescence from the streptavidin captured BCA reflects the quantity of the target molecule in the sample. This aptamer combination strategy based on the SBA holds good potential for applications in simultaneous detection and separation of targets of aptamers or certain DNA and RNA targets.
authors: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, China.
authors: Tao
authors: T
authors: Bing
authors: Xiangjun
authors: X
authors: Liu
authors: Xiaohong
authors: X
authors: Cheng
authors: Zehui
authors: Z
authors: Cao
authors: Dihua
authors: D
authors: Shangguan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin