title: Improvement in the antifilarial efficacy of doxycycline and rifampicin by combination therapy and drug delivery approach.
abstract: The present investigation deals with the evaluation of antifilarial efficacy of liposome entrapped antiwolbachial antibiotics doxycycline and rifampicin (5 doses at 10 mg/kg, subcutaneously for 15 days) alone and/or in combination with standard filaricide diethylcarbamazine (DEC) against human lymphatic filariid Brugia malayi in rodent host Mastomys coucha. The delivery system maintained the sustained release of antibiotics up to 48 h and significantly (P < 0.05) augmented the antifilarial potential of these antibiotics over their free administration. A combination of DEC with each entrapped antibiotics significantly (P<0.05) improved microfilaricidal efficacy, while marginal enhancement was noticed in adulticidal activity. Combination of both antibiotics formulation with DEC demonstrated marginal increase in macrofilaricidal efficacy; however, it was highest ( approximately 75%).
authors: Division of Parasitology, Central Drug Research Institute, Lucknow, India.
authors: Anil
authors: A
authors: Dangi
authors: Varun
authors: V
authors: Dwivedi
authors: Satish
authors: S
authors: Vedi
authors: Mohammad
authors: M
authors: Owais
authors: Shailja
authors: S
authors: Misra-Bhattacharya
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000302:isolation & purification*; D004049:Diethylcarbamazine / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004318:Doxycycline / Q000008:administration & dosage / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005368:Filariasis / Q000188:drug therapy* / Q000469:parasitology; D005369:Filaricides / Q000008:administration & dosage / Q000627:therapeutic use*; D008081:Liposomes; D008297:Male; D051189:Murinae; D012293:Rifampin / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D005369:Filaricides; D008081:Liposomes; D004318:Doxycycline; D004049:Diethylcarbamazine; D012293:Rifampin