[Determination of diflubenzuron and triflumuron residues in greasy wool by accelerated solvent extraction technique and high performance liquid chromatography].
A method for the determination of diflubenzuron and triflumuron residues in greasy wool was developed by high performance liquid chromatography (HPLC) coupled with accelerated solvent extraction (ASE). The diflubenzuron and triflumuron residues were extracted with acetonitrile saturated with n-hexane at 80 degrees C and 10.34 MPa. The extract was pretreated by a series of procedures such as freezing-lipid filtration, concentration and purification by solid-phase extraction prior to the determination with HPLC. The target analytes were separated on a Waters Atlants dC18 column (250 mm x 4.6 mm, 5 microm), gradiently eluted with acetonitrile and water as the mobile phases and detected by a photodiode array detector (DAD) at 254 nm. The linear ranges were 0.1 - 10.0 mg/L. There were good linearity between the peak areas and concentrations in the linear range for the analytes, and the correlation coefficients of diflubenzuron and triflumuron were higher than 0.9999. The limits of quantification for diflubenzuron and triflumuron were 0.05 and 0.04 mg/kg (S/N > or = 10), respectively. The method is simple, rapid, sensitive and suitable for preliminary screening of diflubenzuron and triflumuron residues in greasy wool.
Fan
Yuanmu
Y
Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo, China. fym1112@sina.com
Huang
Shaotang
S
Yu
Xuejun
X
Gu
Xiaojun
X
Qiu
Yajun
Y
Chen
Shubing
S
Fang
Keteng
K
Chen
Jun
J
D000818:Animals; D001549:Benzamides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004132:Diflubenzuron / Q000032:analysis*; D007306:Insecticides / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D012756:Sheep; D012997:Solvents / Q000032:analysis / Q000737:chemistry; D014935:Wool / Q000737:chemistry*
D001549:Benzamides; D007306:Insecticides; D010573:Pesticide Residues; D012997:Solvents; C023957:triflumuron; D004132:Diflubenzuron