title: Simultaneous analysis of fourteen tertiary amine stimulants in human urine for doping control purposes by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry.
abstract: A method for the simultaneous screening and confirmation of the presence of fourteen tertiary amine stimulants in human urine by gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated. Solid phase extraction (SPE) and liquid-liquid extraction (LLE) approaches were utilized for the pre-treatment of the urine samples. The study indicated that the capillary temperature played a significant role in the signal abundances of the protonated molecules of cropropamide and crotethamide under positive ion electrospray ionization (ESI) conditions. In addition, comparison studies of two different pre-treatment approaches as well as the two ionization modes were conducted. The LODs of the developed method for all the analytes were lower than the minimum required performance limit (MRPL) as set forth in the World Anti-Doping Agency (WADA) technical document for laboratories. The human urine sample obtained after oral administration of prolintane.HCl was successfully analyzed by the developed method, which demonstrated the applicability and reliability of the method for routine doping control analysis.
authors: National Doping Control Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors: Jianghai
authors: J
authors: Lu
authors: San
authors: S
authors: Wang
authors: Ying
authors: Y
authors: Dong
authors: Xiaobing
authors: X
authors: Wang
authors: Shuming
authors: S
authors: Yang
authors: Jianli
authors: J
authors: Zhang
authors: Jing
authors: J
authors: Deng
authors: Yang
authors: Y
authors: Qin
authors: Youxuan
authors: Y
authors: Xu
authors: Moutian
authors: M
authors: Wu
authors: Gangfeng
authors: G
authors: Ouyang
mesh_terms: D000588:Amines; D000818:Animals; D002491:Central Nervous System Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D000588:Amines; D002491:Central Nervous System Agents