Automated high-throughput cell microsurgery on-chip.
Laser cell kill is an established tool for studying cells' roles during development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of Caenorhabditis elegans and software for image processing and automation, allowing for high-throughput cell ablations.
Chung
Kwanghun
K
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Lu
Hang
H
D000818:Animals; D001331:Automation; D017173:Caenorhabditis elegans / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008866:Microsurgery / Q000295:instrumentation / Q000379:methods; D012984:Software