title: Polymorphism and signatures of selection in the multimammate rat DQB gene.
abstract: In order to test if DQB is a good candidate marker to investigate the relationship between major histocompatibility complex genes and pathogens in natural populations of Mastomys natalensis, we assessed the polymorphism and evolutionary history of this gene. Twenty-four individuals were genotyped for exon 2 of DQB using capillary electrophoresis single-strand conformation polymorphism, cloning, and sequencing. We found 21 different alleles. Four individuals show three alleles implying a duplication event in the history of this gene. Each distinct sequence translates to give a distinct amino acid sequence and there are strong signals of positive selection on peptide binding sites. Signals of recombination were found in the sequences suggesting that recombination has played a role in generating allelic diversity. Although trans-taxon polymorphism is present at the interspecific level in DQB exon 2 sequences of Mus species, we did not find any evidence of allele sharing among Muridae genera. This indicates a temporal limit of DQB allele sharing in Muridae of less than 8 Mya. In conclusion, although DQB seems to be a good marker to investigate pathogen-driven selection, the polymorphism of gene copy number may restrict its utility in natural populations.
authors: Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Joelle.GouydeBellocq@ua.ac.be
authors: Joëlle
authors: J
authors: Goüy de Bellocq
authors: Herwig
authors: H
authors: Leirs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D051189:Murinae / Q000235:genetics*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment