The effect of zinc on hydroxyapatite-mediated activation of human polymorphonuclear neutrophils and bone implant-associated acute inflammation.
Hydroxyapatite (HA) is widely used as coating biomaterial for prosthesis metal parts and as bone substitute. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. At the inflamed site, the polymorphonuclear cells (PMNs) represent the earliest phagocytic cells that predominate the cellular infiltrate. We have recently proposed that HA wear debris activate polymorphonuclear cells (PMNs) initiating and/or amplifying thereby the acute inflammatory response. Previous studies have shown that activation of monocytes by HA could be modulated by supplementing this latter with the divalent cation, Zinc. The purpose of this work was to investigate the modulation of PMNs activation following exposure to zinc-substituted HA. Our study demonstrate that addition of zinc to HA particles resulted in decreased levels of the pro-inflammatory mediator interleukin-8 (IL-8) and the matrix metallo-proteinase-9. We also show that these changes involve IL-8 receptors (CXCR-1 and CXCR-2).
Velard
Frédéric
F
INSERM, UMR-S 926, CHU de Reims, IFR 53, URCA-1 avenue du Maréchal Juin, 51095 Reims, Cedex, France. frederic.velard@univ-reims.fr
Laurent-Maquin
Dominique
D
Braux
Julien
J
Guillaume
Christine
C
Bouthors
Sylvie
S
Jallot
Edouard
E
Nedelec
Jean-Marie
JM
Belaaouaj
Abderrazzaq
A
Laquerriere
Patrice
P
D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000276:immunology*; D002633:Chemotaxis / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry / Q000276:immunology*; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D008422:Materials Testing; D020780:Matrix Metalloproteinase 9 / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D023062:Receptors, Interleukin-8A / Q000276:immunology; D023063:Receptors, Interleukin-8B / Q000276:immunology; D015032:Zinc / Q000737:chemistry / Q000276:immunology*
D020099:Coated Materials, Biocompatible; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D017886:Durapatite; D020780:Matrix Metalloproteinase 9; D015032:Zinc