[Curcumine inhibits migration and invasion of hepatic stellate cells by reducing MMP-2 expression and activity].
OBJECTIVE
To investigate the molecular mechanism of the inhibitory effect of curcumine on the migration and invasion of hepatic stellate cells (HSC).


METHODS
Rat hepatic stellate cells were cultured and activated with ConA. Matrix metalloproteinase-2 (MMP-2) expression and activity was determined by Western blot and gelatin zymography. Migration and invasion of HSC was assessed by wound healing assay and modified Boyden chamber assay.


RESULTS
Curcumine reduced the level and activity of MMP-2 expression in activated HSC in a dose-dependent manner. When treated with 25, 50 or 100 micromol/L curcumine, the expression of MMP-2 was reduced by 21.8%+/-5.1%, 65.5%+/-9.2% or 87.9%+/-11.5% (P < 0.05), and the activity of MMP-2 was also significantly reduced by curcumine. Migration and invasion of activated HSC was also inhibited by curcumine in a dose-dependent way. When treated with 25, 50 or 100 micromol/L curcumine, the migration of activated HSC was reduced by 27.5%+/-5.8%, 54.4%+/-7.6% or 67.1%+/-9.3% (P < 0.05), and the invasion of activated HSC was also significantly reduced by curcumine.


CONCLUSIONS
Curcumine inhibits migration and invasion of activated HSC by reducing MMP-2 expression and activity.
Huang
Jian-xian
JX
Department of General Surgery, Bao'an People's Hospital, Affiliated to Nanfang Medical University, Shenzhen Guangdong 518101, China.
Zhu
Bao-he
BH
He
De
D
Huang
Lin
L
Hu
Ke
K
Huang
Bo
B
D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000008:administration & dosage / Q000494:pharmacology; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008103:Liver Cirrhosis / Q000517:prevention & control*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D051381:Rats; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000008:administration & dosage / Q000494:pharmacology*
D003208:Concanavalin A; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D003474:Curcumin