[Effects of Kaempferol on activation, proliferation and cell cycle of mouse T lymphocytes in vitro.].
OBJECTIVE
To study the effects of Kaempferol on activation, proliferation and cell cycle of murine T lymphocytes, and to elucidate the mechanism of the immunosuppressive effect of Kaempferol.


METHODS
Lymphocytes were prepared from lymphoid nodes of mouse, and were stimulated with polyclonal activators ConA, and then were co-cultured with Kaempferol of different final concentration. The effect on the expression of CD69 (the early marker of the activated T cells) on T lymphocytes were measured by flow cytometry combined with two colored monoclonal antibodies flow cytometry. While, the effect of Kaempferol on the proliferation of T lymphocytes in response to ConA stimulation was determined by MTT, cell cycle was detected by PI staining.


RESULTS
Kaempferol (final concentration is 10, 20 and 40 mumol/L) can inhibit the expression of CD69 on activated T lymphocytes in a dose-dependent manner (P<0.05). The expression rate of CD69 on T cells in response to ConA was (39.11+/-1.17)%. After treatment with Kaempferol, the expression rate of CD69 reduced to (30.64+/-0.23)%, (27.95+/-0.04)% and (5.63+/-0.37)%, respectively (P< 0.05); The result of MTT showed that Kaempferol can inhibit the proliferation of T lymphocytes stimulated by ConA in a dose-dependent manner (P<0.05); The result of PI staining showed that Kaempferol can arrest cell cycle at S phage and G2/M at all experimental concentration (P<0.05).


CONCLUSIONS
Kaempferol can effectively inhibit the activation, proliferation of mouse T lymphocytes in respond to ConA, arrest cell cycle at S phage and G2/M in vitro.
Mu
Jing-Jing
JJ
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Zeng
Yao-Ying
YY
Huang
Xiu-Yan
XY
Zhao
Xiao-Hui
XH
Song
Bing
B
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D044949:Kaempferols; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000378:metabolism
D015703:Antigens, CD; D044949:Kaempferols