title: Nongenetic method for purifying stem cell-derived cardiomyocytes.
abstract: Several applications of pluripotent stem cell (PSC)-derived cardiomyocytes require elimination of undifferentiated cells. A major limitation for cardiomyocyte purification is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamine methyl ester perchlorate, could be used to selectively mark embryonic and neonatal rat cardiomyocytes, as well as mouse, marmoset and human PSC-derived cardiomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardiomyocytes transplanted into testes did not induce teratoma formation. Moreover, aggregate formation of PSC-derived cardiomyocytes through homophilic cell-cell adhesion improved their survival in the immunodeficient mouse heart. Our approaches will aid in the future success of using PSC-derived cardiomyocytes for basic and clinical applications.
authors: Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan.
authors: Fumiyuki
authors: F
authors: Hattori
authors: Hao
authors: H
authors: Chen
authors: Hiromi
authors: H
authors: Yamashita
authors: Shugo
authors: S
authors: Tohyama
authors: Yu-Suke
authors: YS
authors: Satoh
authors: Shinsuke
authors: S
authors: Yuasa
authors: Weizhen
authors: W
authors: Li
authors: Hiroyuki
authors: H
authors: Yamakawa
authors: Tomofumi
authors: T
authors: Tanaka
authors: Takeshi
authors: T
authors: Onitsuka
authors: Kenichiro
authors: K
authors: Shimoji
authors: Yohei
authors: Y
authors: Ohno
authors: Toru
authors: T
authors: Egashira
authors: Ruri
authors: R
authors: Kaneda
authors: Mitsushige
authors: M
authors: Murata
authors: Kyoko
authors: K
authors: Hidaka
authors: Takayuki
authors: T
authors: Morisaki
authors: Erika
authors: E
authors: Sasaki
authors: Takeshi
authors: T
authors: Suzuki
authors: Motoaki
authors: M
authors: Sano
authors: Shinji
authors: S
authors: Makino
authors: Shinzo
authors: S
authors: Oikawa
authors: Keiichi
authors: K
authors: Fukuda
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002144:Callithrix; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D017690:Cell Transplantation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000032:analysis; D006321:Heart / Q000196:embryology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008928:Mitochondria / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D051381:Rats; D012235:Rhodamines / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C401833:tetramethylrhodamine methyl ester