RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo.
The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal-Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction. In vitro and in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus.
Conrad
Sabine
S
Department of Experimental Embryology, Institute of Anatomy, Tübingen, Germany.
Stimpfle
Fabian
F
Montazeri
Sonia
S
Oldekamp
Judit
J
Seid
Karin
K
Alvarez-Bolado
Gonzalo
G
Skutella
Thomas
T
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000502:physiology*; D018274:Electroporation / Q000379:methods; D004622:Embryo, Mammalian; D005452:Fluoresceins; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D013234:Stem Cells / Q000502:physiology; D014162:Transfection / Q000379:methods; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D000098551:Prospero-Related Homeobox 1 Protein
D015816:Cell Adhesion Molecules, Neuronal; D005452:Fluoresceins; D058851:GPI-Linked Proteins; D018398:Homeodomain Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C483497:Rgmb protein, mouse; D025521:Tumor Suppressor Proteins; C485184:enhanced green fluorescent protein; C091279:neogenin; D000098551:Prospero-Related Homeobox 1 Protein; C069306:5-chloromethylfluorescein; D049452:Green Fluorescent Proteins