title: Membrane-based hybridization capture of intracellular peptide nucleic acid.
abstract: Peptide nucleic acid (PNA) is a chimeric oligonucleotide with nucleotide-derived bases and a peptide backbone. Compared with natural nucleotides, PNA has several advantages, including improved stability and high sequence discrimination during duplex formation. Despite its potential for therapeutic application, analysis technologies have not been generalized, mainly due to ambiguous physiochemical properties resembling those of nucleic acids as well as protein. Here we present a PNA detection method: sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electrotransfer to a Western blotting membrane and then hybridization with a radiolabeled oligonucleotide probe. This method is useful for evaluating the quality of synthetic PNA and determining its intracellular localization.
authors: Virus Research Laboratory, Mogam Biotechnology Research Institute, Bojeong-dong, Yongin, Gyeonggi-do 449-913, South Korea.
authors: Duckhyang
authors: D
authors: Shin
authors: Minwoo
authors: M
authors: Nam
authors: Yeup
authors: Y
authors: Yoon
authors: Meehyein
authors: M
authors: Kim
mesh_terms: D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids