Interactions of temperature and taste in conditioned aversions.
The influence of temperature on taste cues and the ability to discriminate and learn about different temperatures of foods are important factors regulating ingestion. The goal of this research was to demonstrate that thermal orosensory input can serve as a salient stimulus to guide ingestive behavior in the rat, and also that it interacts with gustatory input during choice and conditioned aversion experiments. A novel apparatus with Peltier refrigerators was used to control the temperature of solutions in 10-min, 2-bottle tests. It was determined that naive rats preferred cold water (10 degrees C) to warm water (40 degrees ). When cold water was paired with a toxic LiCl injection, rats avoided cold water and drank warm water, thus demonstrating that cold water could serve as the conditioned stimulus in a conditioned temperature aversion. Rats conditioned against cold water could discriminate 10 degrees C water from 16 degrees C water, but not from 13 degrees C water, thus showing an ability to discriminate orosensory thermal cues to within 3-6 degrees C. Rats also generalized conditioned aversions from cold water to cold saccharin and cold sucrose solutions. However, if rats were conditioned against a compound taste and thermal stimulus (10 degrees C, 0.125% saccharin), the rats could distinguish and avoid each component individually, i.e., by avoiding cold water or warm saccharin. Finally, daily 2-bottle extinction tests were used to assess the strength of aversions conditioned against a taste cue (0.25 M sucrose), a thermal cue (10 degrees C water), or the combination. Aversions to taste or temperature alone persisted for 7-14 days of extinction testing, but the combined taste-temperature aversion was stronger and did not extinguish after 20 days of extinction testing. These results demonstrate that temperature can serve as a salient cue in conditioned aversions that affect ingestion independent of taste cues or by potentiating taste cues.
Smith
Patrick L
PL
Department of Psychology, Program in Neuroscience, The Florida State University, Tallahassee, FL 32306, USA.
Smith
James C
JC
Houpt
Thomas A
TA
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D003080:Cold Temperature; D003213:Conditioning, Psychological / Q000502:physiology*; D003463:Cues; D004192:Discrimination, Psychological / Q000502:physiology; D005108:Extinction, Psychological; D005247:Feeding Behavior / Q000502:physiology*; D005518:Food Preferences; D006358:Hot Temperature; D018021:Lithium Chloride; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012439:Saccharin; D013395:Sucrose; D013649:Taste / Q000502:physiology*
D013395:Sucrose; D012439:Saccharin; D018021:Lithium Chloride