Silicon photonic wire biosensor array for multiplexed real-time and label-free molecular detection.
We demonstrate a silicon photonic wire waveguide biosensor array chip for the simultaneous monitoring of different molecular binding reactions. The chip is compatible with automated commercial spotting tools and contains a monolithically integrated microfluidic channel for sample delivery. Each array sensor element is a 1.8-mm-long spiral waveguide folded within a 130 microm diameter spot and is incorporated in a balanced Mach-Zehnder interferometer with a near temperature independent response. The sensors are arranged in a 400 microm spacing grid pattern and are addressed through cascaded 1x2 optical power splitters using light from a single input fiber. We demonstrate the real-time monitoring of antibody-antigen reactions using complementary and mismatched immunoglobulin G receptor-analyte pairs and bovine serum albumin. The measured level of detection for each sensor element corresponds to a surface coverage of less than 0.3 pg/mm(2).
Densmore
A
A
Institute for Microstructural Sciences, National Research Council Canada, Ottawa, Ontario, Canada K1A 0R6. adam.densmore@nrc-cnrc.gc.ca
Vachon
M
M
Xu
D-X
DX
Janz
S
S
Ma
R
R
Li
Y-H
YH
Lopinski
G
G
Delâge
A
A
Lapointe
J
J
Luebbert
C C
CC
Liu
Q Y
QY
Cheben
P
P
Schmid
J H
JH
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D007074:Immunoglobulin G / Q000378:metabolism; D046228:Microarray Analysis; D044085:Microfluidics; D017785:Photons; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012825:Silicon; D013194:Staining and Labeling; D018511:Systems Integration; D013997:Time Factors
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D012825:Silicon