[Effect of simian vacuolating virus 40 on development and differentiation of dendritic cells from Rhesus macaque].
To study the effect of simian vacuolating virus 40 (SV40) on development and differentiation of dendritic cells (DC) from rhesus macaque, the peripheral blood-derived dendritic cells from rhesus monkey were pulsed with inactivated SV40 and infective SV40, respectively at the 5th day post DC cultivation. Expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface at the 7th, 9th day post DC cultivation were analyzed by flow cytometry (FCM). The results showed that expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface in the inactivated SV40-pulsed experimental group were higher than those in the infective SV40-pulsed experimental group (P < 0.05). These cell surface molecules represented characteristic development and differentiation phase of DC. Down-regulation of expressions of these cell surface molecules indicated that infective SV40 might hamper differentiation and maturation of dendritic cells from rhesus monkey.
Ge
Chang-Yong
CY
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
Li
Hong-Jun
HJ
He
Zhan-Long
ZL
Zhang
Guang-Ming
GM
Sun
Mao-Sheng
MS
Wu
Nan-Ping
NP
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000378:metabolism; D027601:Polyomavirus Infections / Q000503:physiopathology; D013539:Simian virus 40 / Q000502:physiology*; D000097802:CD83 Antigen
D015703:Antigens, CD; D018949:Antigens, CD1; D051940:B7-2 Antigen; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen