title: Identification of genes related to the development of bamboo rhizome bud.
abstract: Bamboo (Phyllostachys praecox) is one of the largest members of the grass family Poaceae, and is one of the most economically important crops in Asia. However, complete knowledge of bamboo development and its molecular mechanisms is still lacking. In the present study, the differences in anatomical structure among rhizome buds, rhizome shoots, and bamboo shoots were compared, and several genes related to the development of the bamboo rhizome bud were identified. The rice cross-species microarray hybridization showed a total of 318 up-regulated and 339 down-regulated genes, including those involved in regulation and signalling, metabolism, and stress, and also cell wall-related genes, in the bamboo rhizome buds versus the leaves. By referring to the functional dissection of the homologous genes from Arabidopsis and rice, the putative functions of the 52 up-regulated genes in the bamboo rhizome bud were described. Six genes related to the development of the bamboo rhizome bud were further cloned and sequenced. These show 66-90% nucleotide identity and 68-98% amino acid identity with the homologous rice genes. The expression patterns of these genes revealed significant differences in rhizome shoots, rhizome buds, bamboo shoots, leaves, and young florets. Furthermore, in situ hybridization showed that the PpRLK1 gene is expressed in the procambium and is closely related to meristem development of bamboo shoots. The PpHB1 gene is expressed at the tips of bamboo shoots and procambium, and is closely related to rhizome bud formation and procambial development. To our knowledge, this is the first report that uses rice cross-species hybridization to identify genes related to bamboo rhizome bud development, and thereby contributes to the further understanding of the molecular mechanism involved in bamboo rhizome bud development.
authors: State Key Laboratory of Plant Physiology and Biochemistry, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, College of Life Sciences, Zhejiang University, Hangzhou, China.
authors: Kuihong
authors: K
authors: Wang
authors: Huazheng
authors: H
authors: Peng
authors: Erpei
authors: E
authors: Lin
authors: Qunying
authors: Q
authors: Jin
authors: Xiqi
authors: X
authors: Hua
authors: Sheng
authors: S
authors: Yao
authors: Hongwu
authors: H
authors: Bian
authors: Ning
authors: N
authors: Han
authors: Jianwei
authors: J
authors: Pan
authors: Junhui
authors: J
authors: Wang
authors: Mingjuan
authors: M
authors: Deng
authors: Muyuan
authors: M
authors: Zhu
mesh_terms: D000595:Amino Acid Sequence; D031723:Bambusa / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000145:classification / Q000235:genetics; D027343:Rhizome / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins