title: Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes.
abstract: Half-smooth tongue sole (Cynoglossus semilaevis) is an increasingly important aquaculture species in China. It is also a tractable model to study sex chromosome evolution and to further elucidate the mechanism of sex determination in teleosts. Two bacterial artificial chromosome (BAC) libraries for C. semilaevis, with large, high-quality inserts and deep coverage, were constructed in the BamHI and HindIII sites of the vector pECBAC1. The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents. The combined libraries have a greater than 99% probability of containing any single-copy sequence. Screening high-density arrays of the libraries with probes for female-specific markers and sex-related genes generated between 4-46 primary positive clones per probe. Thus, the two BAC libraries of C. semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong, 266071, China.
authors: Chang-Wei
authors: CW
authors: Shao
authors: Song-Lin
authors: SL
authors: Chen
authors: Chantel F
authors: CF
authors: Scheuring
authors: Jian-Yong
authors: JY
authors: Xu
authors: Zhen-Xia
authors: ZX
authors: Sha
authors: Xiao-Li
authors: XL
authors: Dong
authors: Hong-Bin
authors: HB
authors: Zhang
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D005398:Fisheries / Q000379:methods*; D005412:Flatfishes / Q000145:classification / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D015723:Gene Library; D008297:Male; D019849:Sex Determination Processes / Q000235:genetics*