Inhibition of Bovine herpesvirus multiplication in MDBK cells by small interfering RNAs.
Small interfering RNA (siRNA) mediated gene silencing is a promising approach in antiviral therapy. To investigate the antiviral effects of siRNAs on Bovine herpesvirus 1 (BoHV-1) multiplication, we designed and in vitro synthesized two siRNAs (siRNA-1 and siRNA-2) targeting the UL25 gene that is essential for BHV-1 multiplication. siRNA-1 and siRNA-2 inhibited the BoHV-1 multiplication in MDBK cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (TCID(50)/ml) determined in cell culture. This indicates that, in general, siRNAs can inhibit BHV-1 multiplication in vitro and could be used also against a BHV-1 infection in vivo following optimization of their application.
Narute
P S
PS
Division of Animal Biotechnology, Undian Veterinary Research Institute, Izatnagar, 243 122, India.
Raut
A A
AA
Saini
M
M
Rai
A
A
Gupta
P K
PK
D000818:Animals; D002417:Cattle; D002460:Cell Line; D006566:Herpesviridae Infections / Q000628:therapy; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology* / Q000627:therapeutic use; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
D034741:RNA, Small Interfering; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus