title: The zebrafish lens proteome during development and aging.
abstract: OBJECTIVE
  Changes in lens protein expression during zebrafish development results in a smooth gradient of refractive index necessary for excellent optical function. Age-related changes in crystallin expression have been well documented in mammals but are poorly understood in the zebrafish.
  
  
  METHODS
  In the zebrafish lens, a systematic analysis of protein content with age was performed using size exclusion chromatography (SEC) combined with linear trap quadrupole Fourier transform tandem mass spectrometry (LTQ-FT LC-MS/MS; rank-order shotgun) proteomics in lenses of larval, juvenile, and adult zebrafish.
  
  
  RESULTS
  alpha-Crystallins, previously shown to have low abundance in the zebrafish lens, were found to increase dramatically with maturation and aging. SEC determined that beta-crystallin was predominant at 4.5 days. With age, the alpha- and gamma-crystallins increased, and a high molecular weight fraction appeared between six weeks and six months to become the dominant component by 2.5 years. Similarly, shotgun proteomics determined that beta-crystallins were the predominant proteins in the young lens. With age, the proportion of alpha- and gamma-crystallins increased dramatically. After crystallins, calpain 3, membrane, and cytoskeletal proteins were most abundant. Five new beta-crystallins and 13 new gamma-crystallins were identified.
  
  
  CONCLUSIONS
  As expected, SEC and proteomics demonstrated changing levels of protein expression with age, especially among the crystallins. The results also confirmed the existence of novel crystallins in the zebrafish genome.
authors: Department of Biological Structure, University of Washington, Seattle, WA 98195-7420, USA.
authors: Teri M S
authors: TM
authors: Greiling
authors: Scott A
authors: SA
authors: Houck
authors: John I
authors: JI
authors: Clark
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D020029:Base Pairing; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D002875:Chromosomes / Q000378:metabolism; D003459:Crystallins / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D013058:Mass Spectrometry; D010802:Phylogeny; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003459:Crystallins; D020543:Proteome