Electro-optical imaging microscopy of dye-doped artificial lipidic membranes.
Artificial lipidic bilayers are widely used as a model for the lipid matrix in biological cell membranes. We use the Pockels electro-optical effect to investigate the properties of an artificial lipidic membrane doped with nonlinear molecules in the outer layer. We report here what is believed to be the first electro-optical Pockels signal and image from such a membrane. The electro-optical dephasing distribution within the membrane is imaged and the signal is shown to be linear as a function of the applied voltage. A theoretical analysis taking into account the statistical orientation distribution of the inserted dye molecules allows us to estimate the doped membrane nonlinearity. Ongoing extensions of this work to living cell membranes are discussed.
Hajj
Bassam
B
Laboratoire de Photonique Quantique et Moléculaire, Centre National de la Recherche Scientifique, UMR8537, d'Alembert Institute, Ecole Normale Supérieure de Cachan, Cachan, France. bassamhajj85@hotmail.com
De Reguardati
Sophie
S
Hugonin
Loïc
L
Le Pioufle
Bruno
B
Osaki
Toshihisa
T
Suzuki
Hiroaki
H
Takeuchi
Shoji
S
Mojzisova
Halina
H
Chauvat
Dominique
D
Zyss
Joseph
J
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008564:Membrane Potentials; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques; D008853:Microscopy; D057054:Molecular Imaging; D055620:Optical Phenomena; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D011199:Potentiometry; D011726:Pyridinium Compounds / Q000378:metabolism
C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D004396:Coloring Agents; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011726:Pyridinium Compounds