Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering.
Bone-marrow-derived mesenchymal stem cells (BMSCs) are a promising source of cells for tissue engineering due to their multilineage mesenchymal differentiation potential. Their ability to proliferate and differentiate into the osteogenic, chondrogenic, and adipogenic lineage makes them an attractive cell source as compared to the terminally differentiated cells. In tissue engineering, use of cell sheet technology is gaining popularity. It is based on culturing cells until hyperconfluence, and it has resulted in the reduction of the number of cells lost when seeding onto scaffolds. Thus, formation of cell sheets with multipotent cells, such as BMSCs, would be a promising alternative to the conventional method of cell seeding, that is, single-cell suspension. However, the multilineage potential of BMSC cell sheets has yet to be verified. Therefore, the aim of this study was to characterize the formation of a hyperconfluent BMSC cell sheet as well as the effects of the hyperconfluent culture conditions on the multipotentiality of BMSCs. Our results showed that the BMSC cell sheets remained viable. The cell sheets were rich with type I collagen and were shown to have retained their multipotentiality. Hence, the use of BMSC cell sheets for tissue engineering application seems promising.
See
Eugene Yong-Shun
EY
National University of Singapore, Singapore, Singapore.
Toh
Siew Lok
SL
Goh
James Cho Hong
JC
D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D020219:Chondrogenesis / Q000235:genetics; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D055758:SOX9 Transcription Factor / Q000235:genetics; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D047495:PPAR gamma; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor