Lichen metabolites modulate hydrogen peroxide and nitric oxide in mouse macrophages.
The activities of perlatolic acid (1), atranorin (2), and lecanoric acid (3) and their derivatives, such as orsellinates and beta-methyl orsellinates obtained by alcoholysis, were assessed for stimulation of the release of hydrogen peroxide and nitric oxide in cultures of peritoneal macrophage cells from mice. The hydrogen peroxide production was estimated by oxidation of phenol red, while the Griess reagent was used to determine the nitric oxide production. 1 and 4-methoxy-ethyl orsellinate (XVII) were the compounds that induced the greatest release of H2O2, whereas n-pentyl orsellinate (IV), iso-propyl orsellinate (V), sec-butyl orsellinate (VI), and XVII induced a small release of NO. These results indicate that lichen products and their derivatives have potential immune-modulating activities.
Carlos
Iracilda Z
IZ
UNESP-São Paulo State University, Faculdade de Ciências Farmacêuticas, C. Postal 502, 14801-902, Araraquara, SP, Brazil. carlosiz@fcfar.unesp.br
Quilles
Marcela B
MB
Carli
Camila B A
CB
Maia
Danielle C G
DC
Benzatti
Fernanda P
FP
Lopes
Thiago I B
TI
Gianini
Aline S
AS
Brum
Rosenei L
RL
Vilegas
Wagner
W
dos Santos
Lourdes C
LC
Honda
Neli K
NK
D000818:Animals; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008011:Lichens / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*
D010936:Plant Extracts; D009569:Nitric Oxide; D006861:Hydrogen Peroxide