A partial nontandem duplication of the MLL gene in four patients with acute myeloid leukemia.
Unusual MLL gene rearrangements were found in bone marrow cells of four patients with acute myeloid leukemia. A combination of conventional and molecular cytogenetic methods were used to describe translocations t(9;12;11)(p22;p13;q23), t(11;19)(q23;p13.3), and t(10;11)(p12;23) and inverted insertion ins(10;11)(p12;q23.3q23.1). Partial nontandem duplication of the MLL gene was identified by reverse transcriptase-polymerase chain reaction in all cases. The duplication, which included MLL exons 2 through 8-9, was interrupted by a cryptic insertion of one or two exons from the respective MLL partner gene: MLLT10, MLLT3, or MLLT1.
Sárová
Iveta
I
Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic. iveta.sarova@uhkt.cz
Brezinová
Jana
J
Zemanová
Zuzana
Z
Lizcová
Libuse
L
Berková
Adéla
A
Izáková
Silvia
S
Malinová
Eva
E
Fuchs
Ota
O
Kostecka
Arnost
A
Provazníková
Dana
D
Filkuková
Jitka
J
Maaloufová
Jacqueline
J
Starý
Jan
J
Michalová
Kyra
K
D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D020440:Gene Duplication; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
D017931:DNA Primers; C497422:KMT2A protein, human; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase