[The effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 concentration in rats].
OBJECTIVE
To investigate the effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 (GLP-1) concentration in rats.


METHODS
Sixty SD rats were randomly divided into four groups, i. e. normal control group (NC group), refed with normal diet group (RN group), high fat diet group (HF group) and refed with high fat diet group (RH group). The weight and diet of rats were recorded. At the onset of refeeding (4 weeks ) and after 2 or 4 weeks of refeeding (6 or 8 weeks), animals were sacrificed and then gastric emptying study test and the assessment of plasma GLP-1 concentration were carried out.


RESULTS
Compared with NC group, rats in HF group possessed accelerated gastric emptying rate (95.61% vs 96.16%, 94.24% vs 96.11%, 93.08% vs 95.50%) and increased GLP-1 concentration (12.02 pmol/L vs 16.27 pmol/L, 10.77 pmol/L vs 13.93 pmol/L, 9.47 pmol/L vs 11.53 pmol/L) at 4, 6, 8 weeks respectively. Similarly, compared with NC group, RN group possessed slowed gastric emptying rate (95.61% vs 87.60%) and decreased GLP-1 concentration (12.02 pmol/L vs 9.69 pmol/L) at 4 weeks, but accelerated gastric emptying rate (94.24% vs 96.11%, 93.08% vs 94.52%) and increased GLP-1 concentration (10.77 pmol/L vs 12.57 pmol/L, 9.47 pmol/L vs 9.80 pmol/L) at 6 and 8 weeks respectively. Compared with HF group, RH group obtained slowed gastric emptying rate (96.16% vs 90.70%) and decreased GLP-1 concentration (16.27 pmol/L vs 9.69 pmol/L) at 4 weeks, but accelerated gastric emptying rate(96.11% vs 98.02%) and slightly lower GLP-1 concentration (13.93 pmol/L vs 13.44 pmol/L) at 6 weeks, and further slowed gastric emptying rate (96.50% vs 92.57%) and lower GLP-1 concentration (11.53 pmol/L vs 10.00 pmol/L).


CONCLUSIONS
Although both high-fat diet and catch-up growth affect the gastrointestinal function, the latter has a greater impact, which could account for some pathophysiological changes caused by catch-up growth.
Chen
Lu-Lu
LL
Department of Endocrinology, Xiehe Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China. cheria_chen@126.com
Yang
Wei-Hong
WH
Zheng
Juan
J
Yue
Ling
L
Hu
Xiang
X
D000818:Animals; D004041:Dietary Fats; D005746:Gastric Emptying; D052216:Glucagon-Like Peptide 1 / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004041:Dietary Fats; D052216:Glucagon-Like Peptide 1