title: Comparison of real-time PCR methods for measurement of HIV-1 proviral DNA.
abstract: The use of HIV-1 DNA quantitation in cellular reservoirs to predict disease progression and treatment outcome in infected patients is hampered by the lack of standardization among the available methods. In the present study, real-time PCR methods used commonly for HIV-1 proviral DNA evaluation were compared, showing strong differences in the results, probably as a consequence of genome variability in the target regions. Standardization of HIV-1 proviral DNA quantitation assays is needed for use in clinical management of patients with HIV-1.
authors: INMI L Spallanzani, Rome, Italy.
authors: G
authors: G
authors: Rozera
authors: I
authors: I
authors: Abbate
authors: A
authors: A
authors: Bruselles
authors: B
authors: B
authors: Bartolini
authors: G
authors: G
authors: D'Offizi
authors: E
authors: E
authors: Nicastri
authors: C
authors: C
authors: Tommasi
authors: M R
authors: MR
authors: Capobianchi
mesh_terms: D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D019562:Viral Load
chemical_list: D004279:DNA, Viral