title: [Screening of anti-Cry1Ac scFv from A phage display antibody library.].
abstract: OBJECTIVE
  To clone human anti-Cry1Ac single-chain antibodies (scFv) from Tomlinson J phage antibody library.
  
  
  METHODS
  A human large phage antibody library was panned for four rounds of "adhesion-elution-amplification". The specificity of the antigen binding activity of the selected clones was identified by ELISA and the variable genes were analyzed and identified.
  
  
  RESULTS
  After the panning, 2 positive clones were obtained, which could bind Cry1Ac specifically. DNA sequence analysis showed that they had different antibody V region encoding genes.
  
  
  CONCLUSIONS
  By-passing immunization, the specific human anti-Cry1Ac scFv also can be prepared by using phage antibody library.
authors: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors: Yun
authors: Y
authors: Wang
authors: Yuan
authors: Y
authors: Liu
authors: Xiang
authors: X
authors: Wang
authors: Ya-Ping
authors: YP
authors: Qian
authors: Xian-Jin
authors: XJ
authors: Liu
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000276:immunology
chemical_list: D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D057127:Single-Chain Antibodies