Surveillance of HIV type 1 drug resistance among naive patients from Venezuela.
We have studied 65 HIV-1-infected untreated patients recruited in Caracas, Venezuela with TCD4 counts > or =350/microl. The reverse transcriptase and protease sequences of the virus were sequenced, aligned with reference HIV-1 group M strains, and analyzed for drug resistance mutations. Most of the viruses were subtype B genotype in both the protease and RT genomic regions. Five of the 62 virus isolates successfully amplified showed evidence of recombination between protease and RT, with their protease region being non-B while their RT region was derived from subtype B. Four strains were found bearing resistance mutations either to NRTIs, NNRTIs, or PIs. The prevalence of HIV-1 isolates bearing resistance mutations was therefore above the 5% threshold of WHO.
Castillo
Julio
J
Department of Internal Medicine, Vargas Hospital, Central University of Venezuela, Caracas, Venezuela.
Comegna
Mario
M
Quijada
Wilmary
W
Jauvin
Valérie
V
Pinson
Patricia
P
Masquelier
Bernard
B
Fleury
Hervé
H
Castro
Erika
E
D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015995:Prevalence; D011995:Recombination, Genetic; D014687:Venezuela / Q000453:epidemiology; D055815:Young Adult
D054303:HIV Reverse Transcriptase; D016333:HIV Protease