Lactate damages primary hippocampal neurons in vitro.
In the present study, rat primary cultures were used to study the effect of lactate on the survival of hippocampal neurons in the presence or absence of glucose. Our results showed no extensive cell damage under glucose-free conditions compared with glucose-rich conditions. Addition of 10 and 50 mM lactate to glucose-free and glucose-rich media increased the cell damage significantly, as observed by morphology and lactate dehydrogenase activity. The results of the present study suggest that primary neurons in vitro are not sensitive to glucose deficiency and the presence of lactate damages the neurons in a concentration-dependent manner.
Sepehr
Arian
A
Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping 58185, Sweden.
Mohseni
Simin
S
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D005947:Glucose / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D045022:Lactate Dehydrogenases / Q000378:metabolism; D007773:Lactates / Q000633:toxicity*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D007773:Lactates; D045022:Lactate Dehydrogenases; D005947:Glucose