Minimum length of homology arms required for effective Red/ET recombination.
The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.
Fujimoto
Rika
R
Graduate School of Innovative Life Science, University of Toyama, Japan.
Osakabe
Toshihiko
T
Saito
Masumichi
M
Kurosawa
Nobuyuki
N
Isobe
Masaharu
M
D001483:Base Sequence; D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000191:economics / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid