title: Evaluation of the combination of the NucliSENS easyMAG and the EasyQ applications for the detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in respiratory tract specimens.
abstract: Mycoplasma pneumoniae and Chlamydia pneumoniae are respiratory tract pathogens frequently involved in community-acquired pneumonia, but are fastidious microorganisms. Their direct detection mainly requires molecular amplification techniques. A nucleic acid extraction system, NucliSENS easyMAG, and a real-time nucleic acid sequence-based amplification (NASBA) technique, NucliSENS EasyQ, were recently developed by bioMérieux to detect both bacteria. The aim of our study was to compare the easyMAG/EasyQ combination with our in-house combination, MagNA Pure extraction (Roche) and real-time polymerase chain reaction (PCR), to detect both bacteria in respiratory tract specimens. The analytical specificities of both combinations were similar. A higher analytical sensitivity was found for C. pneumoniae using the easyMAG/EasyQ combination, since the easyMAG/EasyQ system detected nucleic acid extracts 10(6) times more diluted than the in-house combination. Both combinations were equivalent when detecting M. pneumoniae in positive respiratory tract samples. Finally, the easyMAG/EasyQ combination is a potential useful tool for the detection of both bacteria regarding sensitivity, specificity, monitoring, and standardization of the procedure.
authors: Laboratoire de Bactériologie, CHU de Bordeaux, 33076 Bordeaux cedex, France.
authors: E
authors: E
authors: Béssède
authors: H
authors: H
authors: Renaudin
authors: M
authors: M
authors: Clerc
authors: B
authors: B
authors: de Barbeyrac
authors: C
authors: C
authors: Bébéar
authors: S
authors: S
authors: Pereyre
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001826:Body Fluids / Q000382:microbiology; D023521:Chlamydophila Infections / Q000175:diagnosis* / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic