title: The contribution of systems biology and reverse genetics to the understanding of Kaposi's sarcoma-associated herpesvirus pathogenesis in endothelial cells.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 is the causative agent of the endothelial cell-derived tumour Kaposi's sarcoma. Herpesviruses possess large complex genomes which provide many options to regulate cellular physiology during the viral life cycle and in the course of tumourigenicity. Novel techniques of systems biology and reverse genetics are increasingly applied to dissect the complex interaction of KSHV with endothelial cells. This review will outline novel results and pitfalls of these technologies in the elucidation of KSHV pathogenicity.
authors: Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany. michael.stuerzl@uk-erlangen.de
authors: Michael
authors: M
authors: Stürzl
authors: Andreas
authors: A
authors: Konrad
authors: Khaled R
authors: KR
authors: Alkharsah
authors: Ramona
authors: R
authors: Jochmann
authors: Mathias
authors: M
authors: Thurau
authors: Gaby
authors: G
authors: Marquardt
authors: Thomas F
authors: TF
authors: Schulz
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000821:virology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000209:etiology / Q000235:genetics / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D040901:Proteomics; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000235:genetics / Q000821:virology; D015398:Signal Transduction; D049490:Systems Biology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins