title: [Study on effects of baicalin, berberine and Astragalus polysaccharides and their combinative effects on aldose reductase in vitro].
abstract: OBJECTIVE
  To observe the activities of baicalin, berberine and Astragalus polysaccharides and their combinative effects on aldose reductase (AR) by a screening model of aldose reductase inhibitor (ARI) in vitro.
  
  
  METHODS
  The inhibition of AR by baicalin, berberine and Astragalus polysaccharides and positive drug (Epalrestat) in different concentrations were evaluated, and their combinative effects were studied according to orthogonal t design.
  
  
  RESULTS
  Baicalin and berberine had remarkable inhibitory effects on AR, the inhibitory rates were (88.4 +/- 7.4)% and (69.0 +/- 9.4)% at the concentration of 300 microg/mL. However, the combinative effect of the inhibition on AR by the two compounds was antagonistic action. Astragalus polysaccharides had no activity of inhibition on AR.
  
  
  CONCLUSIONS
  Baicalin and berberine are the potential AR inhibitors as they can inhibit the activity of AR in vitro.
authors: Center for Chinese Medical Therapy and Systems Biology, Shanghai University of TCM, Shanghai 211203, China.
authors: Hong-Zhou
authors: HZ
authors: Yang
authors: Ming-Mei
authors: MM
authors: Zhou
authors: Ai-Hua
authors: AH
authors: Zhao
authors: Shu-Ning
authors: SN
authors: Xing
authors: Zi-Quan
authors: ZQ
authors: Fan
authors: Wei
authors: W
authors: Jia
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D027885:Astragalus propinquus / Q000737:chemistry; D001599:Berberine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D048909:Diabetes Complications / Q000201:enzymology / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D011134:Polysaccharides; D001599:Berberine; C038044:baicalin; D000449:Aldehyde Reductase