title: Insulin-like growth factor-II and insulin-like growth factor-binding proteins in bovine cystic ovarian disease.
abstract: Cystic ovarian disease (COD) is one of the most common reproductive disorders of cattle and is considered to have multifactorial aetiology. An accepted hypothesis involves neuroendocrinological dysfunction of the hypothalamic-pituitary-gonadal axis; however, the role of growth factors in COD has not been extensively investigated. The present study examines the potential role of members of the insulin-like growth factor (IGF) family in COD. Expression of genes encoding IGF-II and insulin-like growth factor-binding proteins (IGFBPs) was examined and the distribution of IGF-II within the follicular wall was assessed immunohistochemically. Finally, the concentration of IGF-II protein was determined in follicular fluid. There was increased IGF-II mRNA in the wall of cystic follicles, mainly associated with granulosa cells. Additionally, there was significantly more IGF-II protein in granulosa and theca cells in cystic follicles, but no change in the concentration of IGF-II in follicular fluid. Total IGFBPs, assessed by western blotting, were similar in different structures. However, by discriminating each IGFBP a decrease was detected in IGFBP-2 expression in cystic follicles that may be related to the observed higher expression of IGF-II. In summary, the present study provides evidence to suggest that COD in cattle is associated with modifications in the IGF-II system.
authors: Departamento de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina.
authors: F
authors: F
authors: Rey
authors: F M
authors: FM
authors: Rodríguez
authors: N R
authors: NR
authors: Salvetti
authors: M M
authors: MM
authors: Palomar
authors: C G
authors: CG
authors: Barbeito
authors: N S
authors: NS
authors: Alfaro
authors: H H
authors: HH
authors: Ortega
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry; D006107:Granulosa Cells / Q000378:metabolism / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010048:Ovarian Cysts / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II