Effect of poly(amido)amine (PAMAM) G4 dendrimer on heart and liver mitochondria in an animal model of diabetes.
Diabetes-induced injury related to hyperglycaemia is associated with impaired function of mitochondria. Regardless of their cytotoxicity, PAMAM [poly(amido)amine] G4 dendrimers lower plasma glucose and suppress long-term markers of diabetic hyperglycaemia in experimental diabetes. In the present study, we aimed at verifying whether such modulatory effects of PAMAM G4 (0.5 micromol/kg of body weight daily for 60 days) may contribute to improved respiration in heart and liver mitochondria from streptozotocin-diabetic rats. PAMAM G4 alleviated long-term markers of hyperglycaemia and reduced blood and tissue lipophilic antioxidants in diabetic animals, but did not restore mitochondrial function. In hearts, but not livers, dendrimers further reduced respiratory function and oxidative phosphorylation. Thus ameliorating effects of PAMAM G4 on glycation and glycoxidation in experimental diabetes are not sufficient to restore the impaired mitochondrial function in diabetes.
Labieniec
Magdalena
M
Department of General Biophysics, University of Lodz, 12/16 Banacha Street, 90-237 Lodz, Poland. magdalab@biol.uni.lodz.pl
Ulicna
Olga
O
Vancova
Olga
O
Kucharska
Jarmila
J
Gabryelak
Teresa
T
Watala
Cezary
C
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D050091:Dendrimers / Q000627:therapeutic use*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009757:Nylons; D010085:Oxidative Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D024505:Tocopherols / Q000494:pharmacology; D014451:Ubiquinone / Q000494:pharmacology
D000975:Antioxidants; D001786:Blood Glucose; D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D014451:Ubiquinone; D024505:Tocopherols