Identification of Riemerella anatipestifer genes differentially expressed in infected duck livers by the selective capture of transcribed sequences technique.
Riemerella anatipestifer is the causative agent of duck septicaemia. Determination of R. anatipestifer virulence mechanisms will help us to effectively control this contagious agent. The differentially expressed gene profile of R. anatipestifer in infected duck livers was therefore identified and compared with in vitro cultures by selective capture of transcribed sequences analysis. A total of 48 genes were identified, of which 43 were genes that encode enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins and secreted proteinases. Five were unknown, novel genes. Eight genes representing the categories were randomly chosen and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by R. anatipestifer in infected duck livers, with changes ranging from 1.44-fold to 4.62-fold compared with in vitro cultures. The results from the present study revealed a gene expression profile of R. anatipestifer in infected duck livers. The unknown but novel genes may be potential novel virulence factors for R. anatipestifer. In conclusion, the data from this study will provide a molecular basis for further study of R. anatipestifer pathogenesis.
Zhou
Zutao
Z
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Zheng
Juan
J
Tian
Wenxia
W
Li
Jianli
J
Zhang
Wanpo
W
Zhang
Jinliang
J
Meng
Xianrong
X
Hu
Sishun
S
Bi
Dingren
D
Li
Zili
Z
D000818:Animals; D004372:Ducks; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D008099:Liver / Q000378:metabolism* / Q000382:microbiology; D010325:Pasteurella / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D010326:Pasteurella Infections / Q000235:genetics / Q000662:veterinary*; D011201:Poultry Diseases / Q000235:genetics* / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics
D037521:Virulence Factors