[Biological characteristics of a chimeric rabies virus expressing canine parvovirus VP2 protein].
To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury (VP2) grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells. Experiments on the mice immunized with the chimeric virus HEP-Flury (VP2) demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.
Niu
Xue-Feng
XF
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Liu
Xiao-Hui
XH
Sun
Zhao-Jin
ZJ
Shi
He-He
HH
Chen
Jing
J
Jiang
Bido
B
Sun
Jing-Chen
JC
Guo
Xiao-Feng
XF
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D051379:Mice; D017993:Parvovirus, Canine / Q000235:genetics* / Q000276:immunology; D011818:Rabies / Q000276:immunology / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D036022:Capsid Proteins; D014765:Viral Vaccines