Heterologous cross-expression of oxygenase and glycosyltransferase genes in streptomycetes, producing angucyclic antibiotics.
The heterologous expression of oxygenase genes urdM, ovmOIII, lanM2, IndZ5 and glycosyltransferase gene urdGT2 in different angucycline producers of genus Streptomyces has been carried out. The introduction of genes urdM, lanM2 and urdGT2 results in the accumulation of new glycosylated compounds in several strains under investigation. A number of processed recombinant strains display streptomycin sensitivity and decrease in total antibacterial activity. The obtained data is an evidence of changes in antibiotics production, resulting from post-polyketide synthase (post-PKS) tailoring gene expression across streptomycete strains. Our study demonstrates the potential of post-PKS tailoring genes for generation of novel bioactive metabolites.
Kobylyanskyy
A
A
Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Lviv, Ukraine. natureson@rambler.ru
Ostash
B
B
Fedorenko
V
V
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000737:chemistry; D001551:Benz(a)Anthracenes / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D003433:Crosses, Genetic; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D001551:Benz(a)Anthracenes; D010105:Oxygenases; D016695:Glycosyltransferases