title: [Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus].
abstract: OBJECTIVE
  Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus.
  
  
  METHODS
  Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains.
  
  
  RESULTS
  33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%.
  
  
  CONCLUSIONS
  MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.
authors: Wuhan Institute of Biological Products, Wuhan, China.
authors: Xia
authors: X
authors: Wang
authors: Ge-Lin
authors: GL
authors: Xu
authors: Jie
authors: J
authors: Wu
authors: Jing
authors: J
authors: Luo
authors: Jia-Hong
authors: JH
authors: Zhu
authors: Jian-Jun
authors: JJ
authors: Zhou
authors: Nan-Feng
authors: NF
authors: Shi
authors: Qing
authors: Q
authors: Tang
authors: Yong-Zhen
authors: YZ
authors: Zhang
authors: Jian-Rong
authors: JR
authors: Tang
authors: Tai-Cai
authors: TC
authors: Wu
authors: He-Tian
authors: HT
authors: Ming
authors: Rui-Hua
authors: RH
authors: Shen
authors: Huan-Chun
authors: HC
authors: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005240:Feasibility Studies; D005455:Fluorescent Antibody Technique / Q000379:methods; D051379:Mice; D011820:Rabies virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity