title: Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies.
abstract: Recent studies have identified a conserved "core" of proteins that are required for centriole duplication. A small number of additional proteins have recently been identified as potential duplication factors, but it is unclear whether any of these proteins are components of the core duplication machinery. In this study, we investigate the function of one of these proteins, Drosophila melanogaster Ana3. We show that Ana3 is present in centrioles and basal bodies, but its behavior is distinct from that of the core duplication proteins. Most importantly, we find that Ana3 is required for the structural integrity of both centrioles and basal bodies and for centriole cohesion, but it is not essential for centriole duplication. We show that Ana3 has a mammalian homologue, Rotatin, that also localizes to centrioles and basal bodies and appears to be essential for cilia function. Thus, Ana3 defines a conserved family of centriolar proteins and plays an important part in ensuring the structural integrity of centrioles and basal bodies.
authors: The Gurdon Institute, Cambridge CB2 1QN, England, UK.
authors: Naomi R
authors: NR
authors: Stevens
authors: Jeroen
authors: J
authors: Dobbelaere
authors: Alan
authors: A
authors: Wainman
authors: Fanni
authors: F
authors: Gergely
authors: Jordan W
authors: JW
authors: Raff
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D002502:Centrioles / Q000378:metabolism / Q000648:ultrastructure*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D051379:Mice
chemical_list: C546573:Ana3 protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; C466102:rotatin protein, mouse