In vivo selection of tumor-targeting RNA motifs.
In an effort to target the in vivo context of tumor-specific moieties, we screened a large library of nuclease-resistant RNA oligonucleotides in tumor-bearing mice to identify candidate molecules with the ability to localize to hepatic colon cancer metastases. One of the selected molecules is an RNA aptamer that binds to p68, an RNA helicase that has been shown to be upregulated in colorectal cancer.
Mi
Jing
J
Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA.
Liu
Yingmiao
Y
Rabbani
Zahid N
ZN
Yang
Zhongguang
Z
Urban
Johannes H
JH
Sullenger
Bruce A
BA
Clary
Bryan M
BM
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D003110:Colonic Neoplasms / Q000473:pathology* / Q000628:therapy*; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005821:Genetic Techniques; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000556:secondary* / Q000628:therapy*; D051379:Mice; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy*; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D012313:RNA; D053487:DEAD-box RNA Helicases