HDAC3 augments the autoregulation of neuroD gene in P19 cells.
NeuroD, a basic helix-loop-helix transcription factor, is capable of converting embryonic epidermal cells into neuronal cells. However, whether histone deacetylases (HDACs) are involved in the autoregulation of neuroD or not is unclear. In this study, neuroD expression was found to be significantly increased in the all-trans retinoid acid-treated P19 cells. Meanwhile, neuroD could itself enhance its promoter activity and mRNA expression. By using specific inhibitors to histone modification enzymes, HDAC3 was identified to specifically augment the autoactivation of neuroD in P19 cells. The data suggest that the elevation of HDAC3 and neuroD in all-trans retinoid acid-treated cells exponentially increases the neuroD expression and mediates an early commitment of P19 cells for neuronal differentiation.
Fang
Hong-Bo
HB
National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Mi
Yang
Y
Zhang
Ye
Y
Wu
Ning-Hua
NH
Shen
Yu-Fei
YF
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006655:Histone Deacetylases / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection / Q000379:methods; D014212:Tretinoin / Q000494:pharmacology; D000099186:Histone Deacetylase 3
D051792:Basic Helix-Loop-Helix Proteins; D004791:Enzyme Inhibitors; D006655:Histone Deacetylases; D008156:Luciferases; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014212:Tretinoin; D000099186:Histone Deacetylase 3; C093564:Neurogenic differentiation factor 1