title: Putrescine-polysaccharide conjugates as transglutaminase substrates and their possible use in producing crosslinked films.
abstract: Putrescine (1,4-diaminobutane) was covalently linked to alginate and low-methoxyl pectin to synthesize new aminated polysaccharides. Both putrescine-pectin and -alginate conjugates, although the latter at higher concentrations, were found to be able to act as effective acyl acceptor transglutaminase substrates in vitro using both dimethylated casein and soy flour proteins as acyl donors. Monodansylcadaverine, a well known acyl acceptor transglutaminase substrate, dose-dependently counteracted the covalent binding of the aminated polysaccharides to the proteins. Putrescine-pectin conjugate was also tested to prepare, in combination with soy flour proteins, edible films in the presence of purified microbial transglutaminase. Characterization of the enzymatically crosslinked films showed a significant decreased water vapor permeability, with respect to the ones obtained with non-aminated pectin in the presence of transglutaminase, as well as improved mechanical properties, such as high extensibility. Possible biotechnological applications of hydrocolloid films containing putrescine-polysaccharide derivatives enzymatically crosslinked to proteins were suggested.
authors: Department of Food Science, University of Naples "Federico II", Portici, Naples, Italy.
authors: Prospero
authors: P
authors: Di Pierro
authors: Loredana
authors: L
authors: Mariniello
authors: Angela
authors: A
authors: Sorrentino
authors: Reynaldo
authors: R
authors: Villalonga
authors: Belkis
authors: B
authors: Chico
authors: Raffaele
authors: R
authors: Porta
mesh_terms: D000464:Alginates / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D001709:Biotechnology; D003432:Cross-Linking Reagents / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D011700:Putrescine / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity; D011503:Transglutaminases / Q000737:chemistry
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D011134:Polysaccharides; D030262:Soybean Proteins; D010368:Pectins; D020723:Glucuronic Acid; D011503:Transglutaminases; D011700:Putrescine