title: Fermentation supernatants of wheat ( Triticum aestivum L.) aleurone beneficially modulate cancer progression in human colon cells.
abstract: Wheat aleurone contains high amounts of dietary fibers that are fermented by the microflora, resulting in the formation of short-chain fatty acids (SCFA), which are recognized for their chemopreventive potential. This study investigated the effects of fermented aleurone on growth, apoptosis, differentiation, and expression of several genes using two different human colon cell lines (LT97 and HT29). In LT97 cells, the fermentation supernatant (fs) aleurone reduced significantly the cell growth (EC(50) after 48 h = 7.6-8.3%), whereas the level of apoptotic cells was significantly increased (2.1-2.3-fold). Differentiation was enhanced in HT29 cells (1.8-fold) more than in LT97 cells (1.6-fold). Cell growth and apoptosis-related genes, namely WNT2B and p21, were induced by the fs (LT97, 1.7-3.3-fold; HT29, 7.9-22.2-fold). In conclusion, fermented wheat aleurone is able to act as a secondary chemopreventive agent by modulating parameters of cell growth and survival, whereas cells of an early transformation stage are more sensitive.
authors: Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University Jena, Dornburger Strasse 24, 07743 Jena, Germany. Anke.Borowicki@uni-jena.de
authors: Anke
authors: A
authors: Borowicki
authors: Katrin
authors: K
authors: Stein
authors: Daniel
authors: D
authors: Scharlau
authors: Michael
authors: M
authors: Glei
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D004043:Dietary Fiber / Q000494:pharmacology*; D005232:Fatty Acids, Volatile / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D009385:Neoplastic Processes; D014908:Triticum / Q000737:chemistry*
chemical_list: D004043:Dietary Fiber; D005232:Fatty Acids, Volatile