Proteome differences of placenta between pre-eclampsia and normal pregnancy.
Placenta is a tissue unique to pregnancy and despite its major role in pregnancy, little is known about the proteome changes within placenta during pregnancy-related diseases such as pre-eclampsia (PE). Therefore, the aim of this study is the analysis of proteome differences between pre-eclamptic and normal full-term placentas. To achieve this goal, five normal and five severe pre-eclamptic placentas were included in this study. Total placental proteins were extracted and subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). After staining, the gels were scanned and the protein spots were analysed using Image Master 2D Platinum Software. Non-parametric Mann-Whitney test was used for analysis of the mean intensity differences of the spots between normal and pre-eclamptic placentas. Statistical analysis indicated that 17 spots were differently expressed in pre-eclamptic compared with normal placentas (p<0.05). Using Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF/TOF) mass analysis, 11 out of 17 spots were identified. Among them, four proteins (chloride intracellular channel 3, apolipoprotein A-I, transthyretin (TTR) and protein disulphide isomerase) were up-regulated while seven (peroxiredoxin 2, peroxiredoxin 3, Hsc 70, Cu/Zn-superoxide dismutase (SOD-1), actin gamma 1 propeptide, chain A of enoyl-coenzyme A hydratase and HSP gp96) showed decreased expression in PE in comparison with normal placentas. In conclusion, down-regulation of proteins with anti-oxidant activities (peroxiredoxin 2 and peroxiredoxin 3) and altered expression of stress-response proteins (Hsc 70, Hsp gp96 and protein disulphide isomerase) might play an important role in the pathogenesis of PE.
Gharesi-Fard
B
B
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Zolghadri
J
J
Kamali-Sarvestani
E
E
D000328:Adult; D002585:Cesarean Section; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050883:HSC70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D047929:Term Birth; D015854:Up-Regulation; D055815:Young Adult
D050883:HSC70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D008562:Membrane Glycoproteins; D011257:Pregnancy Proteins; C053387:endoplasmin