ICPLQuant - A software for non-isobaric isotopic labeling proteomics.
The main goal of many proteomics experiments is an accurate and rapid quantification and identification of regulated proteins in complex biological samples. The bottleneck in quantitative proteomics remains the availability of efficient software to evaluate and quantify the tremendous amount of mass spectral data acquired during a proteomics project. A new software suite, ICPLQuant, has been developed to accurately quantify isotope-coded protein label (ICPL)-labeled peptides on the MS level during LC-MALDI and peptide mass fingerprint experiments. The tool is able to generate a list of differentially regulated peptide precursors for subsequent MS/MS experiments, minimizing time-consuming acquisition and interpretation of MS/MS data. ICPLQuant is based on two independent units. Unit 1 performs ICPL multiplex detection and quantification and proposes peptides to be identified by MS/MS. Unit 2 combines MASCOT MS/MS protein identification with the quantitative data and produces a protein/peptide list with all the relevant information accessible for further data mining. The accuracy of quantification, selection of peptides for MS/MS-identification and the automated output of a protein list of regulated proteins are demonstrated by the comparative analysis of four different mixtures of three proteins (Ovalbumin, Horseradish Peroxidase and Rabbit Albumin) spiked into the complex protein background of the DGPF Proteome Marker.
Brunner
Achim
A
Max-Planck-Institute of Biochemistry, Martinsried, Germany. brunner@biochem.mpg.de
Keidel
Eva-Maria
EM
Dosch
Dominik
D
Kellermann
Josef
J
Lottspeich
Friedrich
F
D000418:Albumins / Q000032:analysis; D000818:Animals; D002645:Chickens; D006735:Horseradish Peroxidase / Q000032:analysis; D007553:Isotope Labeling; D010047:Ovalbumin / Q000032:analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D011817:Rabbits; D012985:Software Design; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000418:Albumins; D020543:Proteome; D010047:Ovalbumin; D006735:Horseradish Peroxidase