Protein O-glycosylation induces collagen expression and contributes to diabetic cardiomyopathy in rat cardiac fibroblasts.
Diabetic cardiomyopathy may be accompanied by myocardial fibrosis. We have previously reported that cardiac fibrosis and protein O-glycosylation are elevated in diabetes. In this study, we examined if the hexosamine biosynthesis pathway (HBP) was involved with collagen expression in rat cardiac fibroblasts (RCFs). Long-term glucose load significantly increased type III collagen expression in RCFs, but did not affect the protein O-glycosylation. In addition, glucosamine treatment not only induced expressions of collagen types I and III, but also increased the O-glycosylated protein. These results suggest that O-glycosylation of protein induced by HBP activation modifies collagen expression and contributes to diabetic cardiomyopathy.
Kohda
Yuka
Y
Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Osaka 569-1094, Japan. ykohda@gly.oups.ac.jp
Kanematsu
Miwa
M
Kono
Tatsuji
T
Terasaki
Fumio
F
Tanaka
Takao
T
D000818:Animals; D009202:Cardiomyopathies / Q000150:complications / Q000378:metabolism*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects*; D006595:Hexosamines / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D024042:Collagen Type I; D024061:Collagen Type III; D006595:Hexosamines; D005947:Glucose; D005944:Glucosamine