Microwave-assisted extraction for the simultaneous determination of Novolac glycidyl ethers, bisphenol A diglycidyl ether, and its derivatives in canned food using HPLC with fluorescence detection.
A microwave-assisted extraction (MAE) protocol and an efficient HPLC analysis method were first developed for the fast extraction and simultaneous determination of bisphenol F diglycidyl ether (Novolac glycidyl ether 2-Ring), Novolac glycidyl ether 3-Ring, Novolac glycidyl ether 4-Ring, Novolac glycidyl ether 5-Ring, Novolac glycidyl ether 6-Ring, bisphenol A diglycidyl ether, bisphenol A (2,3-dihydroxypropyl) glycidyl ether, bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether, bisphenol A bis(3-chloro-2-hydroxypropyl) ether, bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) ether in canned fish and meat. After being optimized in terms of solvents, microwave power and irradiation time, MAE was selected to carry out the extraction of ten target compounds. Analytes were purified by poly(styrene-co-divinylbenzene) SPE columns and determined by HPLC-fluorescence detection. LOD varied from 0.79 to 3.77 ng/g for different target compounds based on S/N=3; LOQ were from 2.75 to 10.92 ng/g; the RSD for repeatability were <8.64%. The analytical recoveries ranged from 70.46 to 103.44%. This proposed method was successfully applied to 16 canned fish and meat, and the results acquired were in good accordance with the studies reported. Compared with the conventional liquid-liquid extraction and ultrasonic extraction, the optimized MAE approach gained the higher extraction efficiency (20-50% improved).
Zhang
Hong
H
College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou, PR China.
Xue
Ming
M
Lu
Yanbin
Y
Dai
Zhiyuan
Z
Wang
Honghai
H