title: Effects of aging and every-other-day feeding on the levels of oxygen radicals in rat brain slices.
abstract: Caloric and food restriction attenuate oxidative stress. The effect of aging and every-other-day (EOD) feeding on oxygen radical-dependent chemiluminescent intensity was examined in ex vivo brain slices from Fischer rats during oxygenation and hypoxia-reoxygenation with lucigenin, a chemilumigenic probe used for detecting superoxide anion radicals. The chemiluminescent intensity increased during reoxygenation after hypoxic treatment, and the chemiluminescence in the brain slices at the baseline and during reoxygenation increased with age. However, no difference was observed in the superoxide-dependent chemiluminescence between brain slices prepared from the aged rats fed EOD and those fed ad libitum. Our results indicated that age-dependent increases in superoxide production might be associated with enhanced oxidative stress in aged Fischer rat brains. However, the present study newly indicated that decreased superoxide production might not be a major causal factor in caloric and food restriction attenuated oxidative stress.
authors: Research Team for Mechanism of Aging, Redox Research, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi, Tokyo 173-0015, Japan. tsasaki@center.tmig.or.jp
authors: Toru
authors: T
authors: Sasaki
authors: Koh-ichi
authors: K
authors: Sato
authors: Izumi
authors: I
authors: Umeda
authors: Shoichi
authors: S
authors: Tahara
authors: Takao
authors: T
authors: Kaneko
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D031204:Caloric Restriction; D004435:Eating; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D049449:Luminescence; D018384:Oxidative Stress; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides