A new antimicrobial peptide isolated from Oudneya africana seeds.
Oudneya africana R. Br. (Brassicaceae), a wild-growing plant in the arid region of Tunisia, is used in ethno-medicinal treatment of microbial infections. Validation of ethno-therapeutic claims pertaining to the plant was sought by investigating its antimicrobial activity. A proteinaceous extract of the seeds, called AS-3000, showed activity against various organisms including L. monocytogenes, E. coli, B. subtilis, E. hirae, P. aeruginosa, S. aureus and C. albicans. Extract AS-3000 exhibited a synergistic effect against L. ivanovii when combined with vancomycin or chloramphenicol. The post-antibiotic inhibitory effect of the ampicillin/AS-3000 combination was 2.3-fold greater than for the antibiotic alone. The mode of action of AS-3000 on Listeria and Escherichia was visible using SEM. These results support the use of O. africana for treating microbial infections.
Hammami
Riadh
R
Unity of Functional Proteomics and Food Biopreservation, Higher Institute of Applied Biological Sciences of Tunis, University Tunis El Manar, Tunis, Tunisia.
Hamida
Jeannette Ben
JB
Vergoten
Gérard
G
Lacroix
Jean-Marie
JM
Slomianny
Marie-Christine
MC
Mohamed
Neffati
N
Fliss
Ismail
I
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D019607:Brassicaceae / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D016983:Enterococcus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008087:Listeria / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011402:Pronase / Q000037:antagonists & inhibitors; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D014416:Tunisia
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010455:Peptides; D010936:Plant Extracts; D014361:Trypsin Inhibitors; D011402:Pronase