Methods and algorithms for relative quantitative proteomics by mass spectrometry.
Protein quantitation by mass spectrometry (MS) is attractive since it is possible to obtain both the identification and quantitative values of novel proteins and their posttranslational modifications in one experiment. In contrast, protein arrays only provide quantitative values of targeted proteins and their modifications. There are an overwhelming number of quantitative mass spectrometry (MS) methods for protein and peptide quantitation. The aim here is to provide an overview of the most common MS-based quantitative methods used in the proteomics field and discuss the computational algorithms needed for the robust quantitation of proteins, peptides, and their posttranslational modifications.
Matthiesen
Rune
R
Instituto de Patologia e Imunologia Molecular da Universidad do Porto - IPATIMUP, Porto, Portugal.
Carvalho
Ana Sofia
AS
D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012984:Software
D010455:Peptides