[Experimental study on effect of hirudin in inhibiting hyperplastic scar fibroblasts].
OBJECTIVE
To study the effect of hirudin on the function of human hyperplastic scar fibroblasts (HSFBs).


METHODS
HSFBs were cultured in vitro. Hirudin solution in the concentration of 1, 10, and 50 kU/L was respectively added into DMEM culture medium to form 1, 10, and 50 kU/L hirudin groups, with 9 wells in each group. HSFBs cultured without hirudin were set up as control group. Cell inhibition rate, secretion level of TGF-beta1 from cells, and expression levels of mRNA of type I and III precollagen were determined at 24, 48, and 72 h after culture.


RESULTS
Inhibition rates of HSFBs growth was respectively (29.3 +/- 0.9)%, (30.1 +/- 0.3)%, and (45.2 +/- 1.9)% when cultured with 10 kU/L hirudin for 24, 48, and 72 hs, which were higher than those in control group [(0.0 +/- 0.0)%, P < 0.05]. There was statistically significant difference between control group and 1 and 50 kU/L hirudin groups in the inhibition rates of HSFBs at some time points (P < 0.05). Secretion level of TGF-beta1 of HSFBs in 1, 10, 50 kU/L hirudin groups was respectively (228.5 +/- 1.8), (210.5 +/- 11.1), and (168.5 +/- 14.1) pg/mL when cultured for 48 hs, of which the last 2 figures were significantly lower than that of control group [(265.0 +/- 1.5) pg/mL, P < 0.05]. Hirudin in the concentration of 10 and 50 kU/L could inhibit the expression of mRNA of type I and III precollagen in HSFBs.


CONCLUSIONS
Hirudin solution in the concentration of 10 and 50 kU/L can inhibit the proliferation of HSFBs and secretion of TGF-beta1 and collagen in certain degree.
LIU
Da-en
DE
Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
LI
Xuan
X
ZHANG
Guo-you
GY
NIU
Zhan-guo
ZG
YI
Cheng-gang
CG
JIA
Yu-bo
YB
XIA
Wei
W
GUO
Shu-zhong
SZ
D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D006629:Hirudins; D053773:Transforming Growth Factor beta1