title: Cigarette smoke condensate causes a decrease of the gene expression of Cu-Zn superoxide dismutase, Mn superoxide dismutase, glutathione peroxidase, catalase, and free radical-induced cell injury in SH-SY5Y human neuroblastoma cells.
abstract: Cigarette smoking condensate (CSC) contains oxidant compounds able to generate superoxide. The aim of the present study was to investigate the effect of the exposure to CSC on: (1) free radical production, (2) the gene expression of the antioxidant enzymes Cu-Zn superoxide dismutase (SOD1), Mn superoxide dismutase (SOD2), Glutathione Peroxidase (GPx), and catalase (CAT), and (3) cell survival in human neuroblastoma SH-SY5Y cells. The results showed that exposure (24 h) to different concentrations (10-150 μg/ml) of CSC caused a dose dependent cell injury that was coupled to the maximal increase of free radical production. These events were prevented by the addition to the incubation medium of the scavenger Vitamin E (50 μM). Furthermore, CSC exposure caused a reduction of the gene expression of the antioxidant enzymes SOD1, SOD2, GPx, and CAT that was counteracted by Vitamin E (50 μM). These results suggest that CSC exposure can induce a free radical overcharge that may be responsible for the inhibition of antioxidant enzymes expression and cell injury in SH-SY5Y human neuroblastoma cells. In fact the scavenger vitamin E can block both cell injury and inhibition of SOD1, SOD2, GPx, and CAT induced by CSC exposure.
authors: Division of Pharmacology, Department of Neuroscience, School of Medicine, "Federico II" University of Naples, Via S. Pansini 5, 80131 Naples, Italy.
authors: Michela
authors: M
authors: Russo
authors: Stefania
authors: S
authors: Cocco
authors: Agnese
authors: A
authors: Secondo
authors: Annagrazia
authors: A
authors: Adornetto
authors: Antonella
authors: A
authors: Bassi
authors: Alfredo
authors: A
authors: Nunziata
authors: Giuliano
authors: G
authors: Polichetti
authors: Bruna
authors: B
authors: De Felice
authors: Simona
authors: S
authors: Damiano
authors: Rosalba
authors: R
authors: Serù
authors: Paolo
authors: P
authors: Mondola
authors: Gianfranco
authors: G
authors: Di Renzo
mesh_terms: D002374:Catalase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D005609:Free Radicals / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000502:physiology; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D052638:Particulate Matter / Q000633:toxicity*; D012906:Smoke / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000072105:Superoxide Dismutase-1; D014026:Nicotiana / Q000009:adverse effects; D014810:Vitamin E / Q000494:pharmacology; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D005609:Free Radicals; D005979:Glutathione Peroxidase; D052638:Particulate Matter; D012906:Smoke; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D014810:Vitamin E; D000099156:Superoxide Dismutase 2; C000606290:SOD1 protein, human