title: Design and evaluation of a real-time PCR assay for quantification of JAK2 V617F and wild-type JAK2 transcript levels in the clinical laboratory.
abstract: The somatic mutation JAK2 V617F is associated with BCR-ABL1-negative myeloproliferative neoplasms. Detection of this mutation aids diagnosis of these neoplasms, and quantification of JAK2 V617F may provide a method to monitor response to therapy. For these reasons, we designed a clinical assay that uses allele-specific PCR and real-time detection with hydrolysis probes for the quantification of JAK2 V617F, wild-type JAK2, and GAPDH transcripts. Mutant and wild-type JAK2 were quantified by using external plasmid standards that contain the relevant JAK2 V617F or JAK2 sequence, respectively. We tested 55 peripheral blood specimens from patients with suspected myeloproliferative neoplasms and 55 peripheral blood specimens from patients not known to have myeloproliferative neoplasms. Low-level, nonspecific amplification was detected in reactions containing a high copy number of plasmid standards and in specimens from patients not known to have myeloproliferative neoplasms, necessitating the use of a laboratory-established mutant to wild-type cutoff. The limit of detection established by using cell line dilutions is 0.1%, and this method identified three JAK2 V617F-positive patients who were not detected by a less sensitive method. The assay characteristics and our initial evaluation indicate this method can be used for the detection and quantification of JAK2 V617F, which should be useful for diagnosis of myeloproliferative neoplasms and potentially for monitoring minimal residual disease in future trials of therapies targeted to myeloproliferative neoplasms.
authors: Department of Pathology, Stanford University Medical Center, 300 Pasteur Dr, L235, Stanford, CA 94305-5324, USA. jdmerker@stanford.edu
authors: Jason D
authors: JD
authors: Merker
authors: Carol D
authors: CD
authors: Jones
authors: Stephen T
authors: ST
authors: Oh
authors: Iris
authors: I
authors: Schrijver
authors: Jason
authors: J
authors: Gotlib
authors: James L
authors: JL
authors: Zehnder
mesh_terms: D000483:Alleles; D015870:Gene Expression; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D057230:Limit of Detection; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D053614:Janus Kinase 2