[Expression of EphA2 and EphrinA1 in human renal cell carcinoma and its relationship with angiogenesis].
OBJECTIVE
To investigate the expression of EphA2 and EphrinA1 and its relationship with angiogenesis in renal cell carcinoma and its relevance to clinicopathologic features.


METHODS
The expression of the EphA2 and EphrinA1 was detected by immunohistochemistry (IHC) in the tissues samples from 68 renal cell carcinomas and 24 normal kidneys, and quantitatively analyzed. The microvessel density (MVD) was determined by CD34 immunostaining of microvascular endothelial cells. Statistical analysis was performed using the software SPSS (version 13.0).


RESULTS
The expression of EphA2, EphrinA1 and MND in the cancerous tissues were significantly higher (P<0.01) than that in the normal ones. Significantly increased expression of EphA2, EphrinA1 and MVD (P<0.01) was detected in cancer tissues with higher grade differentiation, more advanced stage and more lymph node metastasis, respectively (P<0.05 for each group). Expression of the EphA2 and EphrinA1 protein was shown to be positively associated with the MVD assessed by Spearman's correlation and factor analysis (r=0.555, r=0.485, P<0.01). The MVD was also significantly correlated with the diameter of the tumor (P<0.01).


CONCLUSIONS
EphA2 and EphrinA1 are highly expressed in renal cell carcinoma, and positively correlated with histological differentiation, clinical stage and angiogenesis in the cancer.
Xu
Jin-Sheng
JS
Department of Nephrology, The Fourth Hospital of Hebei Medical University, Hebei Provincial Tumor Hospital, Shijiazhuang 050011, China. xujinsheng@medmail.com.cn
Zhang
Jun-Xia
JX
Geng
Tong-Hui
TH
Wang
Yue-Fen
YF
Wang
Xiao-Ling
XL
Zuo
Lian-Fu
LF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000473:pathology; D036382:Ephrin-A1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D036104:Receptor, EphA2 / Q000378:metabolism*; D047368:Tumor Burden; D055815:Young Adult
D036382:Ephrin-A1; D036104:Receptor, EphA2