Spectroscopic study on interaction of bovine serum albumin with sodium magnesium chlorophyllin and its sonodynamic damage under ultrasonic irradiation.
Sonodynamic therapy (SDT) is an attractive antitumor treatment for recent years. In this paper, sodium magnesium chlorophyllin (SMC) as a sonosensitizer combining with ultrasonic (US) irradiation to damage bovine serum albumin (BSA) has been investigated by fluorescence and UV-vis spectroscopy. The interaction of BSA with SMC was studied by the quenching of intrinsic fluorescence at varying temperature. The quenching constants (K(SV)), effective binding constants (K(A)), apparent association constants (K(a)) and binding site numbers were determined. The results indicated the quenching mechanism is a static procedure. Thermodynamic parameters show that the interactions involve hydrogen bonds, hydrophobic interactions, electrostatic interactions and complexations. The binding distance is 3.533 nm. The synergistic effect of SMC and ultrasound was estimated including the study of damage conditions. Synchronous fluorescence spectra indicate the damage to Trp residues is more serious. This paper may offer some valuable references for using spectroscopy method to study the application of chlorophyll derivatives in antitumor treatment.
Wang
Jun
J
Department of Chemistry, Liaoning University, Shenyang, PR China. wangjun890@126.com
Liu
Lijun
L
Liu
Bin
B
Guo
Ying
Y
Zhang
Yuanyuan
Y
Xu
Rui
R
Wang
Shixian
S
Zhang
Xiangdong
X
D000818:Animals; D002417:Cattle; D002735:Chlorophyllides / Q000737:chemistry* / Q000378:metabolism; D017616:Magnesium Compounds / Q000737:chemistry*; D015394:Molecular Structure; D009369:Neoplasms / Q000532:radiotherapy; D011485:Protein Binding / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000378:metabolism; D011838:Radiation-Sensitizing Agents / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D012964:Sodium / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014465:Ultrasonics
D002735:Chlorophyllides; D017616:Magnesium Compounds; D011837:Radiation-Protective Agents; D011838:Radiation-Sensitizing Agents; D012710:Serum Albumin, Bovine; D012964:Sodium; C007020:chlorophyllin