PutidaNET: interactome database service and network analysis of Pseudomonas putida KT2440.
BACKGROUND
Pseudomonas putida KT2440 (P. putida KT2440) is a highly versatile saprophytic soil bacterium. It is a certified bio-safety host for transferring foreign genes. Therefore, the bacterium is used as a model organism for genetic and physiological studies and for the development of biotechnological applications. In order to provide a more systematic application of the organism, we have constructed a protein-protein interaction (PPI) network analysis system of P. putida KT2440.


RESULTS
PutidaNET is a comprehensive interaction database and server of P. putida KT2440 which is generated from three protein-protein interaction (PPI) methods. We used PSIMAP (Protein Structural Interactome MAP), PEIMAP (Protein Experimental Interactome MAP), and Domain-domain interactions using iPfam. PutidaNET contains 3,254 proteins, and 82,019 possible interactions consisting of 61,011 (PSIMAP), 4,293 (PEIMAP), and 30,043 (iPfam) interaction pairs except for self interaction. Also, we performed a case study by integrating a protein interaction network and experimental 1-DE/MS-MS analysis data P. putida. We found that 1) major functional modules are involved in various metabolic pathways and ribosomes, and 2) existing PPI sub-networks that are specific to succinate or benzoate metabolism are not in the center as predicted.


CONCLUSIONS
We introduce the PutidaNET which provides predicted interaction partners and functional analyses such as physicochemical properties, KEGG pathway assignment, and Gene Ontology mapping of P. putida KT2440 PutidaNET is freely available at http://sequenceome.kobic.kr/PutidaNET.
Park
Seong-Jin
SJ
Korean BioInformation Center (KOBIC), KRIBB, Daejeon 305-806, Korea. psj420@kribb.re.kr
Choi
Jong-Soon
JS
Kim
Byoung-Chul
BC
Jho
Seong-Woong
SW
Ryu
Jea-Woon
JW
Park
Daeui
D
Lee
Kyung-A
KA
Bhak
Jong
J
Kim
Seung Il
SI
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D030562:Databases, Protein; D020407:Internet / Q000295:instrumentation; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D012985:Software Design
D001426:Bacterial Proteins; D020543:Proteome