title: HDL2 can inhibit further oxidative modification of partially oxidized LDL.
abstract: OBJECTIVE
  To investigate whether HDL(2) can inhibit further oxidative modification of partially oxidized LDL (ox-LDL) by interrupting the chain oxidation reaction after lipid hydroperoxides (LOOH) formation.
  
  
  METHODS
  Following incubation of LDL 400 microg protein/mL phosphate-buffered saline with Cu(2+) for 1.75 h (defined as 0 min), incubation was continued after adding HDL(2) 200 microg protein/mL or HDL(2) 800 microg protein/mL to give both ox-LDL+HDL(2) 200 microg protein/mL or ox-LDL+HDL(2) 800 microg protein/mL. As a control, ox-LDL 200 microg protein/mL and native LDL were prepared. Each sample was subjected to agarose gel electrophoresis and the LOOH in each sample was measured.
  
  
  RESULTS
  When the electrophoretic mobility of native LDL was designated 1, the relative electrophoretic mobility (REM) of ox-LDL increased significantly over time. The REMs of ox-LDL+HDL(2) 800 microg protein/mL from 10 min to 9 h were significantly lower than the REM of ox-LDL at the respective times (p<0.01). LOOH of ox-LDL+HDL(2) 800 microg protein/mL at 1, 3, 6 and 9 h was significantly higher than LOOH in ox-LDL at the respective times (p<0.01). The results of ox-LDL+HDL(2) 200 microg protein/mL were almost the same but to a lesser extent than the results of ox-LDL+HDL(2) 800 microg protein/mL.
  
  
  CONCLUSIONS
  The present findings suggest that HDL(2) can inhibit further oxidative modification of partially oxidized LDL by interrupting the chain oxidation reaction after LOOH formation in a concentration-dependent manner.
authors: Department of Cardio-Renal Medicine and Hypertension, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. sakuma@med.nagoya-cu.ac.jp
authors: Nagahiko
authors: N
authors: Sakuma
authors: Tomoaki
authors: T
authors: Saeki
authors: Tatsuya
authors: T
authors: Ito
authors: Takeshi
authors: T
authors: Hibino
authors: Shogo
authors: S
authors: Suzuki
authors: Mitoshi
authors: M
authors: Kunimatsu
authors: Koji
authors: K
authors: Yamamoto
authors: Syuichi
authors: S
authors: Kitada
mesh_terms: D003300:Copper / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000096:biosynthesis; D053439:Lipoproteins, HDL2 / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D008054:Lipid Peroxides; D053439:Lipoproteins, HDL2; D008077:Lipoproteins, LDL; D003300:Copper