Evaluation of lead exposure in battery-manufacturing workers with focus on different biomarkers.
The influence of exposure to lead on the frequency of micronuclei (MN), nuclear buds and nucleoplasmatic bridges was investigated in peripheral blood lymphocytes in 15 male battery-manufacturing workers and 15 controls matched for age and smoking habits. In addition to MN test, blood lead (B-Pb) and cadmium (B-Cd), delta aminolevulinic acid dehydratase (ALAD) activity, erythrocyte protoporphyrin (EP), serum vitamin B(12) (S-Vit B(12)) and folate (S-folate) were determined in all subjects. Lead-exposed subjects had significantly higher MN frequency and B-Pb concentrations than controls. In control smokers we found a significant negative correlation between B-Pb concentration and frequency of nucleoplasmatic bridges, and nuclear division index. In control non-smokers a significant positive correlation was observed only between age and nuclear buds frequency, and between S-folate and B-Pb level. In lead exposed smokers, significant positive correlations between MN frequency and S-Vit B(12), S-folate, and nuclear buds frequency were found. A positive correlation in exposed smokers was also found between nuclear buds frequency and S-Vit B(12) concentration. A negative correlation was found between ALAD and EP, and B-Pb in exposed smokers. Exposed non-smokers showed significant negative correlation between MN frequency and B-Cd, and ALAD and EP. The results indicate a genotoxicity of lead, pointing to a micronucleus assay as a relevant test for assessing genotoxic effects resulting from occupational exposure. The other indicators did not necessarily follow the results of THE MN test. Influence of smoking should be further investigated.
Kasuba
Vilena
V
Mutagenesis Unit, Institute for Medical research and Occupational Health, Zagreb, Croatia. vkasuba@imi.hr
Rozgaj
Ruzica
R
Milić
Mirta
M
Zeljezić
Davor
D
Kopjar
Nevenka
N
Pizent
Alica
A
Kljaković-Gaspić
Zorana
Z
D000328:Adult; D015415:Biomarkers / Q000097:blood; D002104:Cadmium / Q000097:blood / Q000633:toxicity; D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism; D005492:Folic Acid / Q000097:blood; D006801:Humans; D007854:Lead / Q000097:blood* / Q000633:toxicity; D008297:Male; D008669:Metallurgy; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis*; D000623:Porphobilinogen Synthase / Q000097:blood; D011524:Protoporphyrins / Q000097:blood; D014805:Vitamin B 12 / Q000097:blood; D055815:Young Adult
D015415:Biomarkers; D011524:Protoporphyrins; D002104:Cadmium; D007854:Lead; D005492:Folic Acid; D000623:Porphobilinogen Synthase; D014805:Vitamin B 12