A discussion concerning the inclusion of variety effect when analysis of variance is used to detect differentially expressed genes.
In microarray studies several statistical methods have been proposed with the purpose of identifying differentially expressed genes in two varieties. A commonly used method is an analysis of variance model where only the effect of interaction between variety and gene is tested. In this paper we argue that in addition to the interaction effects, the main effect of variety should simultaneously also be taken into account when posting the hypothesis.
Feten
Guri
G
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O.Box 5003, N-1432 As, Norway. guri.feten@umb.no
Aastveit
Are Halvor
AH
Snipen
Lars
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Almøy
Trygve
T