title: The effect of pregnancy on paternal skin allograft survival.
abstract: Elucidation of maternal-fetal tolerance mechanisms clarifies the role of regulatory T cells (Treg) in transplant tolerance. This study aim to investigate the effect of pregnancy on paternal skin allograft survival. Flow cytometry techniques, mixed lymphocytes reaction (MLR), PCR, real-time PCR and skin transplantation were key methods. Treg increased significantly from 4.2% before pregnancy to peak at 6.8% day 8 after pregnancy. Both heme oxygenase-1 (HO-1) and indoleamine 2,3-dioxygenase (IDO) mRNA express high in placenta while low in spleen (P<0.05). Although Treg increased during pregnancy, and splenocytes from the pregnant mice showed lower MLR response toward the paternal stimulator, single time pregnancy showed no significant protective effect on paternal skin allograft survival in the tested condition.
authors: Kidney Disease Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. zfshou@zju.edu.cn
authors: ZhangFei
authors: Z
authors: Shou
authors: YiFang
authors: Y
authors: Xu
authors: HuaYing
authors: H
authors: Xiao
authors: Qin
authors: Q
authors: Zhou
authors: JieRu
authors: J
authors: Cai
authors: Yi
authors: Y
authors: Yang
authors: Hong
authors: H
authors: Jiang
authors: WenJie
authors: W
authors: Zhang
authors: JiangHua
authors: J
authors: Chen
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006085:Graft Survival / Q000276:immunology*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy / Q000276:immunology*; D012867:Skin / Q000276:immunology*; D016038:Skin Transplantation / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology*
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D008565:Membrane Proteins; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse