title: [1H-NMR-based metabonomics analysis of plasma from osteoporotic rats induced by ovariectomy].
abstract: OBJECTIVE
  To investigate the application of metabonomics in research of osteoporosis, through detecting change of the endogenous metabolites in plasma from osteoporotic rats by ovariectomy.
  
  
  METHODS
  Six old-months female SD rats were randomly divided into sham and OVX group. Fifth month after ovariectomy, plasma were collected from both groups, respectively. The metabolic profiles were investigated using 1H-NMR spectroscopy of plsama combined with pattern recognition techniques, including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).
  
  
  RESULTS
  The PCA PLS-DA plots of the plasma samples presented marked clustering between sham group and OVX group. Compared to sham group, the level of low molecular metabolites such as lactate, acetone and ethonal were higer, glucose, choline/phosphatidylcholine (Cho/PC), alanine (Ala), high density lipoprotein/low density lipoprotein (HDL/LDL), very low density lipoprotein/low density lipoprotein, fatty acid and glucose were lower.
  
  
  CONCLUSIONS
  Obvious changes in metabonomics of plasma from osteoporotic rats were observed.
authors: Biomedical Engneering Institute, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors: Wei-Fu
authors: WF
authors: Long
authors: Liang
authors: L
authors: Li
authors: Huai-Qing
authors: HQ
authors: Chen
authors: Yong
authors: Y
authors: Tang
authors: Xue-Ling
authors: XL
authors: He
authors: Rong-Zhou
authors: RZ
authors: Jing
mesh_terms: D000818:Animals; D005260:Female; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D010024:Osteoporosis / Q000097:blood* / Q000209:etiology; D010052:Ovariectomy; D010949:Plasma / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley