title: [Sequence analysis and identification of two novel HLA alleles HLA-B*9536 and HLA-B*4612].
abstract: OBJECTIVE
  To identify two novel HLA alleles HLA-B*9536 and B*4612, in an individual.
  
  
  METHODS
  DNA was extracted from whole blood by Invitrogen DNA extraction kit. The amplification for HLA-B exons 2-4 of the proband was performed separately with allele group specific primers and the PCR products were directly sequenced for exons 2-4 in both direction.
  
  
  RESULTS
  There were two novel HLA-B alleles in the proband. The sequences of the two alleles have been submitted to GenBank (EU081878 and EU081879). The two alleles have been officially named as B*9536 and B*4612 by the WHO Nomenclature Committee. The sequence of exons 2-4 of HLA-B*9536 showed one nucleotide difference in exon 3 at position 544 (G to A) comparing with the closest allele B*1505, which resulted in an amino acid change from Ala to Thr at codon 158. In the HLA-B*4612 allele, there was one nucleotide change in exon 3 at position 363 (G to A), when compared to the closest allele B*4601, which lead to an amino acid change from Arg to Ser at codon 97.
  
  
  CONCLUSIONS
  Two novel HLA-B alleles were identified in one individual and have been officially named by the WHO Nomenclature Committee.
authors: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety of Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
authors: Yanmin
authors: Y
authors: He
authors: Wei
authors: W
authors: Zhang
authors: Junjun
authors: J
authors: He
authors: Wei
authors: W
authors: Wang
authors: Zhedong
authors: Z
authors: Han
authors: Nanying
authors: N
authors: Chen
authors: Faming
authors: F
authors: Zhu
authors: Lixing
authors: L
authors: Yan
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D015235:HLA-B Antigens; C530295:HLA-B*15:136 antigen; C530296:HLA-B*46:12 antigen; D059910:HLA-B15 Antigen