Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation.
Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of PDB-MSCs in hypoxia and serum deprivation. PDB-MSCs were cultured in four groups: normoxia (20% O2) and complete medium [10% FBS (foetal bovine serum)+DMEM-HG (Dulbecco's modified Eagle's medium-high glucose)], hypoxia and complete medium, normoxia and serum deprivation (0% FBS), and hypoxia and serum deprivation. After 96 h of culture in the above groups, PDB-MSCs maintain the phenotypes stably. Interestingly, hypoxia notably enhanced the proliferation, colony-forming potential and lactate/glucose ratio in complete medium, but suppressed the secretion of BMP-2 (bone morphogenetic protein-2) and bFGF (basic fibroblast growth factor), while it did not change the quantity of VEGF (vascular endothelial growth factor) and bFGF in serum deprivation. Although PDB-MSCs grew slowly and seldom formed a colony unit in hypoxia and serum deprivation, they possessed a moderate metabolism. In conclusion, our results indicate that PDB-MSCs appear to be promising seed cells for ischaemia-related tissue engineering.
Huang
Yong-Can
YC
Division of Stem cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
Yang
Zhi-Ming
ZM
Jiang
Neng-Gang
NG
Chen
Xiao-He
XH
Li
Xiu-Qun
XQ
Tan
Mei-Yun
MY
Zhou
Kun-Peng
KP
Tang
Li
L
Xie
Hui-Qi
HQ
Deng
Li
L
D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D015687:Cell Hypoxia; D049109:Cell Proliferation; D016895:Culture Media, Serum-Free; D003656:Decidua / Q000166:cytology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D016895:Culture Media, Serum-Free; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2