title: Comparative analysis of selected exhaled breath biomarkers obtained with two different temperature-controlled devices.
abstract: BACKGROUND
  The collection of exhaled breath condensate (EBC) is a suitable and non-invasive method for evaluation of airway inflammation. Several studies indicate that the composition of the condensate and the recovery of biomarkers are affected by physical characteristics of the condensing device and collecting circumstances. Additionally, there is an apparent influence of the condensing temperature, and often the level of detection of the assay is a limiting factor. The ECoScreen2 device is a new, partly single-use disposable system designed for studying different lung compartments.
  
  
  METHODS
  EBC samples were collected from 16 healthy non-smokers by using the two commercially available devices ECoScreen2 and ECoScreen at a controlled temperature of -20 degrees C. EBC volume, pH, NOx, LTB4, PGE2, 8-isoprostane and cys-LTs were determined.
  
  
  RESULTS
  EBC collected with ECoScreen2 was less acidic compared to ECoScreen. ECoScreen2 was superior concerning condensate volume and detection of biomarkers, as more samples were above the detection limit (LTB4 and PGE2) or showed higher concentrations (8-isoprostane). However, NOx was detected only in EBC sampled by ECoScreen.
  
  
  CONCLUSIONS
  ECoScreen2 in combination with mediator specific enzyme immunoassays may be suitable for measurement of different biomarkers. Using this equipment, patterns of markers can be assessed that are likely to reflect the complex pathophysiological processes in inflammatory respiratory disease.
authors: BGFA, Research Institute of Occupational Medicine German Social Accident Insurance, Ruhr-University Bochum, Bochum, Germany. hoffmeyer@bgfa.de
authors: Frank
authors: F
authors: Hoffmeyer
authors: Monika
authors: M
authors: Raulf-Heimsoth
authors: Volker
authors: V
authors: Harth
authors: Jürgen
authors: J
authors: Bünger
authors: Thomas
authors: T
authors: Brüning
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000295:instrumentation*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013696:Temperature
chemical_list: D015415:Biomarkers