SNPs for a universal individual identification panel.
An efficient method to uniquely identify every individual would have value in quality control and sample tracking of large collections of cell lines or DNA as is now often the case with whole genome association studies. Such a method would also be useful in forensics. SNPs represent the best markers for such purposes. We have developed a globally applicable resource of 92 SNPs for individual identification (IISNPs) with extremely low probabilities of any two unrelated individuals from anywhere in the world having identical genotypes. The SNPs were identified by screening over 500 likely/candidate SNPs on samples of 44 populations representing the major regions of the world. All 92 IISNPs have an average heterozygosity [0.4 and the F(st) values are all\0.06 on our 44 populations making these a universally applicable panel irrespective of ethnicity or ancestry. No significant linkage disequilibrium (LD) occurs for all unique pairings of 86 of the 92 IISNPs (median LD = 0.011) in all of the 44 populations. The remaining 6 IISNPs show strong LD in most of the 44 populations for a small subset (7) of the unique pairings in which they occur due to close linkage. 45 of the 86 SNPs are spread across the 22 human autosomes and show very loose or no genetic linkage with each other. These 45 IISNPs constitute an excellent panel for individual identification including paternity testing with associated probabilities of individual genotypes less than 10(-15), smaller than achieved with the current panels of forensic markers. This panel also improves on an interim panel of 40 IISNPs previously identified using 40 population samples. The unlinked status of the subset of 45 SNPs we have identified also makes them useful for situations involving close biological relationships. Comparisons with random sets of SNPs illustrate the greater discriminating power, efficiency, and more universal applicability of this IISNP panel to populations around the world. The full set of 86 IISNPs that do not show LD can be used to provide even smaller genotype match probabilities in the range of 10(-31)-10(-35) based on the 44 population samples studied.
Pakstis
Andrew J
AJ
Department of Genetics, Yale University School of Medicine, 333 Cedar Street, 208005, New Haven, CT 06520, USA.
Speed
William C
WC
Fang
Rixun
R
Hyland
Fiona C L
FC
Furtado
Manohar R
MR
Kidd
Judith R
JR
Kidd
Kenneth K
KK
D002874:Chromosome Mapping / Q000379:methods; D018732:Forensic Anthropology / Q000379:methods*; D005787:Gene Frequency; D005820:Genetic Testing / Q000379:methods; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D006801:Humans; D007206:Individuality; D015810:Linkage Disequilibrium; D010334:Paternity; D010355:Patient Identification Systems / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000502:physiology