title: Dynamic expression and heterogeneous intracellular location of En-1 during late mouse embryonic development.
abstract: Engrailed-1 (En-1) is a transcription factor involved in the development of the midbrain/hindbrain during mouse early embryogenesis. Although En-1 is expressed from embryogenesis to adulthood, there has been no detailed description of its expression during late mouse embryonic development. Here we report the expression pattern of En-1 in the mouse embryo from E10.5 to the neonatal state. With immunohistochemistry we found that En-1 was expressed in the central nervous system (CNS) from E10.5 to the neonatal state, mostly restricted to the midbrain/hindbrain junction. Outside the CNS, En-1 is dynamically expressed in several neural crest-associated structures including the cranial mesenchyme, the mandibular arches, the vagus nerve, the dorsal root ganglia, the sympathetic ganglia, the somites, the heart and the cloaca. Additionally, we found that in the CNS, most of the En-1 was located in the nuclei, while outside the CNS, En-1 was mainly expressed in the cytoplasm. These findings provided additional evidence that En-1 may be involved in the development of neural crest cells.
authors: Department of Histology and Embryology, Third Military Medical University, Chongqing, Republic of China.
authors: Shan-chuan
authors: SC
authors: Zhong
authors: Xing-shu
authors: XS
authors: Chen
authors: Qi-yan
authors: QY
authors: Cai
authors: Xue
authors: X
authors: Luo
authors: Xing-hua
authors: XH
authors: Chen
authors: Jing
authors: J
authors: Liu
authors: Zhong-xiang
authors: ZX
authors: Yao
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism
chemical_list: C080330:En1 protein, mouse; D018398:Homeodomain Proteins