[Evaluation of myocardial ischemia-reperfusion injury in mouse by molecular imaging of P-selectin with targeted contrast echocardiography].
OBJECTIVE
To assess the feasibility of evaluating myocardial ischemia-reperfusion injury in mouse with targeted myocardial contrast echocardiography (MCE).


METHODS
Phospholipid microbubbles targeted to P-selectin (MBp) and control microbubbles (MBc) were created by conjugating monoclonal antibody against murine P-selectin or isotype control antibody with the lipid shell via "avidin-biotin" bridging. Ten mice with myocardial ischemia-reperfusion were injected intravenously of MBp and MBc in a random order with a 30 min interval. After 5 min of intravenous injection of microbubble, targeted MCE imaging was performed in all mice. And then the video intensity (VI) was determined.


RESULTS
A significant ultrasonic enhancement was observed in ischemic region of MBp-group. Increment in VI value of ischemic region in MBp-group was great and it amounted to (26.0 +/- 6.2) U. However, increment in VI value of ischemic region in MBc-group was minor and it was merely (9.1 +/- 0.9) U. Difference was evident in ischemic region between of two groups (P < 0.05). In both MBp-group and MBc-group, the VI value of ischemic region was significantly greater than that of non-ischemic region (6.5 +/- 1.0) U vs (6.4 +/- 0.8) U (P < 0.05). There was no obvious difference in the VI of non-ischemic region between two groups.


CONCLUSIONS
Molecular imaging of P-selectin with targeted contrast echocardiography can effectively evaluate myocardial ischemia-reperfusion injury.
Ji
Li-Jing
LJ
Cardiovascular Division, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Yang
Li
L
Yan
Yi
Y
Wu
Jue-Fei
JF
Li
Jun-Hua
JH
Hu
Guang-Quan
GQ
Bin
Jian-Ping
JP
D000818:Animals; D004562:Electrocardiography / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging*; D019007:P-Selectin; D014463:Ultrasonography
D019007:P-Selectin