Immunohistochemical localization and gene expression of carbonic anhydrase isoenzymes CA-II and CA-VI in canine lower airways and lung.
The immunohistolocalization and gene expression of carbonic anhydrase (CA) isoenzymes CA-II and CA-VI in the canine lower airways and lung were examined using specific canine CA-II and CA-VI antisera and the RT-PCR method. Laryngeal, tracheal and bronchial epithelia, serous acinar and bronchiolar secretory cells and pulmonary great alveolar cells showed immunopositive reactions to anti-CA-II and anti-CA-VI antisera. However, all mucous cells showed immunonegative reactions. The physiological roles of CA-II and CA-VI in the lower airways and lung may involve the maintenance of pH balance and the protection of mucosal surfaces against the acidic milieu.
SUGIURA
Yosuke
Y
Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
OISHI
Motoharu
M
AMASAKI
Tomoko
T
SOETA
Satoshi
S
ICHIHARA
Nobutsune
N
NISHITA
Toshiho
T
MURAKAMI
Masaru
M
AMASAKI
Hajime
H
ASARI
Masao
M
D000818:Animals; D024402:Carbonic Anhydrase II / Q000235:genetics / Q000378:metabolism*; D030741:Carbonic Anhydrase IV / Q000235:genetics / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007527:Isoenzymes; D008168:Lung / Q000201:enzymology*
D007527:Isoenzymes; D024402:Carbonic Anhydrase II; D030741:Carbonic Anhydrase IV