title: [Investigation on the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell degranulation.].
abstract: OBJECTIVE
  To investigate the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell release and search for the suitable index to describe mast cell degranulation.
  
  
  METHODS
  Mast cell degranulation was performed in vitro, in which histamine, tryptase and beta-hexosaminidase are detected by spectrophotometry.
  
  
  RESULTS
  The concordance was found to exit among the three mediums, furthermore the study discloses that more similarity was found between tryptase and beta-hexosaminidase. Histamine was difficult and inconstant to be detected.
  
  
  CONCLUSIONS
  Tryptase and beta-hexosaminidase are more suitable and accurately to reflect mast cell degranulation in vitro than histamine. Tryptase is the excellent index to detect mast cell degranulation in vitro.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors: Yong-Chao
authors: YC
authors: Guo
authors: Zhen-Xing
authors: ZX
authors: Li
authors: Hong
authors: H
authors: Lin
mesh_terms: D015550:Cell Degranulation; D006632:Histamine; D006636:Histamine Release; D006801:Humans; D008407:Mast Cells / Q000276:immunology; D012697:Serine Endopeptidases; D053802:Tryptases; D001619:beta-N-Acetylhexosaminidases
chemical_list: D006632:Histamine; D001619:beta-N-Acetylhexosaminidases; D012697:Serine Endopeptidases; D053802:Tryptases