A G-protein editor gates coenzyme B12 loading and is corrupted in methylmalonic aciduria.
The mechanism by which docking fidelity is achieved for the multitude of cofactor-dependent enzymes is poorly understood. In this study, we demonstrate that delivery of coenzyme B(12) or 5'-deoxyadenosylcobalamin by adenosyltransferase to methylmalonyl-CoA mutase is gated by a small G protein, MeaB. While the GTP-binding energy is needed for the editing function; that is, to discriminate between active and inactive cofactor forms, the chemical energy of GTP hydrolysis is required for gating cofactor transfer. The G protein chaperone also exerts its editing function during turnover by using the binding energy of GTP to elicit release of inactive cofactor that is occasionally formed during the catalytic cycle of MCM. The physiological relevance of this mechanism is demonstrated by a patient mutation in methylmalonyl-CoA mutase that does not impair the activity of this enzyme per se but corrupts both the fidelity of the cofactor-loading process and the ejection of inactive cofactor that forms occasionally during catalysis. Consequently, cofactor in the incorrect oxidation state gains access to the mutase active site and is not released if generated during catalysis, leading, respectively, to assembly and accumulation of inactive enzyme and resulting in methylmalonic aciduria.
Padovani
Dominique
D
Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109-5606, USA.
Banerjee
Ruma
R
D001483:Base Sequence; D002151:Calorimetry; D003038:Cobamides / Q000378:metabolism*; D017931:DNA Primers; D004578:Electron Spin Resonance Spectroscopy; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008661:Metabolism, Inborn Errors / Q000652:urine*; D008764:Methylmalonic Acid / Q000652:urine*; D008765:Methylmalonyl-CoA Mutase / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D013816:Thermodynamics
D003038:Cobamides; D017931:DNA Primers; D006160:Guanosine Triphosphate; D008764:Methylmalonic Acid; D019204:GTP-Binding Proteins; D008765:Methylmalonyl-CoA Mutase; C000913:cobamamide