Widefield fluorescence sectioning with HiLo microscopy.
HiLo microscopy is a widefield fluorescence imaging technique that provides depth discrimination by combining two images, one with non-uniform illumination and one with uniform illumination. We discuss the theory of this technique and a variety of practical implementations in brain-tissue imaging and fluorescence endomicroscopy.
Mertz
Jerome
J
Boston University, Department of Biomedical Engineering, 44 Cummington St., Boston, MA, 02215, USA. jmertz@bu.edu
Lim
Daryl
D
Chu
Kengyeh K
KK
Bozinovic
Nenad
N
Ford
Timothy
T
D000818:Animals; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D015203:Reproducibility of Results