title: Perchlorotrityl radical-fluorophore conjugates as dual fluorescence and EPR probes for superoxide radical anion.
abstract: Perchlorotrityl radicals, mono-substituted with a fluorophore using an amide linker of varying chain length, were synthesized and characterized. Electron paramagnetic resonance (EPR) spectroscopic study indicated free-electron coupling with the aromatic hydrogen nuclei and long-range coupling with the methylene hydrogens of the linker group. Reactivity of the fluorophore-conjugated trityls with superoxide radical anion showed quenching of EPR signal and enhancement of fluorescence emission spectrum. This work presents the first example of a perchlorotrityl-fluorophore conjugate that can potentially be employed as a dual probe for the detection of superoxide under oxidative stress-mediated conditions in biological systems.
authors: Davis Heart and Lung Research Institute, Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA.
authors: Jinhua
authors: J
authors: Wang
authors: Vinh
authors: V
authors: Dang
authors: Wei
authors: W
authors: Zhao
authors: Dongning
authors: D
authors: Lu
authors: Brian K
authors: BK
authors: Rivera
authors: Frederick A
authors: FA
authors: Villamena
authors: Peng George
authors: PG
authors: Wang
authors: Periannan
authors: P
authors: Kuppusamy
mesh_terms: D000577:Amides / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D018384:Oxidative Stress; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013481:Superoxides / Q000138:chemical synthesis / Q000737:chemistry*; D014320:Triphenylmethyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000577:Amides; D000838:Anions; D005456:Fluorescent Dyes; D014320:Triphenylmethyl Compounds; D013481:Superoxides