Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11).
This study was conducted to investigate the amount of CLA synthesized endogenously by rat mammary tissues in response to TVA (a precursor for cis-9, trans-11 CLA endogenous synthesis) treatment as well as the differences in the protein expression of genes encoding the biosynthesis of CLA in rat mammary tissue and mouse mammary gland epithelia cells (HC11). Treatment with TVA resulted in improved CLA productivity. Furthermore, 2-DE revealed two spots in samples of mammary tissues and one spot in samples of mammary gland epithelia cells (HC11) that were consistently altered in the TVA treatment groups when compared with the control group (non-fatty acid). The mRNA expression patterns of three of the proteins (PDI, PRDX2, LAMR1), as measured by real-time PCR, were similar to the pattern of protein abundance. In addition, the expression of SCD mRNA in the mammary tissue of rats and HC11 cell treated with TVA was higher than in the control group. Our results suggest that the identified proteins may be related to CLA biosynthesis in mammary tissue.
Jin
Yong-Cheng
YC
Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 157-921, Republic of Korea.
Lee
Hong-Gu
HG
Xu
Cheng-Xiong
CX
Han
Jeng-A
JA
Choi
Seong-Ho
SH
Song
Man-Kang
MK
Kim
Young-Jun
YJ
Lee
Ki-Beom
KB
Kim
Seon-Ku
SK
Kang
Han-Seok
HS
Cho
Byung-Wook
BW
Shin
Teak-Soon
TS
Choi
Yun-Jaie
YJ
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007774:Lactation / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009829:Oleic Acids / Q000494:pharmacology; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D012333:RNA, Messenger; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; C086117:Rpsa protein, rat; D054464:Peroxiredoxins; D019704:Protein Disulfide-Isomerases; C050413:11-octadecenoic acid