[Simultaneous determination of six flavonoids in Flos Chrysanthemi Indici by RP-HPLC].
OBJECTIVE
To develop an HPLC method for the determination of luteolin-7-O-glycoside, apigenin-7-O-glycoside, linarin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.


METHODS
The reversed phase HPLC system consisting of a C18 column (4.6 mm x 250 mm, 5 microm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted. The absorbance was monitored at 326 nm.


RESULTS
The linear response range was 1.08-21.5 mg x L(-1) (r = 0.9990), 0.278-5.57 mg x L(-1) (r = 0.9994), 5.58-112 mg x L(-1) (r = 0.9999), 0.521-10.4 mg x L(-1) (r = 0.9995), 0.162-3.25 mg x L(-1) (r = 0.9999) and 0.288-5.76 mg x L(-1) (r = 0.9998), respectively (n = 6). The average recoveries (n = 9) of six flavonoids were 96.34%-104.0%. All of RSD of precision (n = 6) and repeatability (n = 6) were less than 1.2% and 2.6%.


CONCLUSIONS
The validation data demonstrated that the method was accurate and repeatable, and can be ase to measure the six flavonoids in Flos Chrysanthemi Indici.
Liu
Fei
F
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. syrliufei820325@hotmail.com
Yang
Yang
Y
Tan
Xiaojie
X
Bi
Kaishun
K
Jia
Ying
Y
D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids