title: Function of Ech hydrogenase in ferredoxin-dependent, membrane-bound electron transport in Methanosarcina mazei.
abstract: Reduced ferredoxin is an intermediate in the methylotrophic and aceticlastic pathway of methanogenesis and donates electrons to membrane-integral proteins, which transfer electrons to the heterodisulfide reductase. A ferredoxin interaction has been observed previously for the Ech hydrogenase. Here we present a detailed analysis of a Methanosarcina mazei Delta ech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduced ferredoxin, indicating an involvement in methanogenesis from methylated C(1) compounds.
authors: Institute of Microbiology and Biotechnology, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany.
authors: Cornelia
authors: C
authors: Welte
authors: Verena
authors: V
authors: Kallnik
authors: Marcel
authors: M
authors: Grapp
authors: Gunes
authors: G
authors: Bender
authors: Steve
authors: S
authors: Ragsdale
authors: Uwe
authors: U
authors: Deppenmeier
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D005288:Ferredoxins / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism*; D017020:Methanosarcina / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D005288:Ferredoxins; D010088:Oxidoreductases; C400203:Ech hydrogenase