title: [Establishment and analysis of chronic periodontitis and atherosclerosis model in Wistar rat].
abstract: OBJECTIVE
  To investigate the possible correlation between atherosclerosis and chronic periodontitis by establishing an animal model of chronic periodontitis and atherosclerosis in Wistar rat.
  
  
  METHODS
  Sixty male Wistar rats were divided into four groups: A (control group), B (chronic periodontitis group), C (atherosclerosis group), D (chronic periodontitis accompany with atherosclerosis group). Every group was accepted the corresponding treatment. Animals were sacrificed after 12 weeks. The periodontal index, levels of serum total cholesterol (TC) and low-density lipoprotein (LDL), the concentration of TNF-alpha and matrix metalloproteinase (MMP-3) were examined. The severity of chronic periodontitis and atherosclerosis was quantified by histopathology. The date were statistically analyzed.
  
  
  RESULTS
  Through detection of periodontal tissue of experimental teeth, serum and histopathology, animal models were successful. Histopathologic observation revealed:obvious inflammation of periodontal tissue was observed in group B and D. Attachment loss level in group B [(137.86 +/- 28.39) microm] and D [(162.36 +/- 22.69) microm] was higher than that in group A [(4.26 +/- 1.07) microm] and C [(68.07 +/- 18.25) microm] (P < 0.05), and that in group C was higher than group A (P < 0.05). Atherosclerotic lesions of abdominal aorta were formed in group C and D. The level of TC, LDL in group C and D was higher than that in group A and B (P < 0.05), and that in group D was higher than group C (P < 0.05). Animals in group B and D showed higher level of TNF-alpha, MMP-3 in serum than that in group A and C (P < 0.05). There was no correlation between the level of MMP-3 and TC (P = 0.971) or LDL (P = 0.604).
  
  
  CONCLUSIONS
  Chronic periodontitis may be a risk factor and contribute to the pathogenesis of atherosclerosis. MMP-3 may be an independent risk factor of atherosclerosis exclude TC and LDL.
authors: Department of Stomatology, Xinjiang Medical University, Urümqi 830054, China.
authors: Liang-Jun
authors: LJ
authors: Zhong
authors: Jun
authors: J
authors: Xu
authors: Yuan-Ming
authors: YM
authors: Zhang
authors: Jia
authors: J
authors: Ni
authors: Xiao-Huan
authors: XH
authors: Zhou
authors: Fang-Chuan
authors: FC
authors: Shi
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D001018:Aortic Diseases / Q000209:etiology* / Q000473:pathology; D050197:Atherosclerosis / Q000209:etiology* / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D055113:Chronic Periodontitis / Q000150:complications* / Q000473:pathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood; D010512:Periodontal Index; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol; D019278:Matrix Metalloproteinase 3