Structure of the X (ADRP) domain of nsp3 from feline coronavirus.
The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
Wojdyla
Justyna A
JA
EMBL Hamburg Outstation, c/o DESY, D-22603 Hamburg, Germany.
Manolaridis
Ioannis
I
Snijder
Eric J
EJ
Gorbalenya
Alexander E
AE
Coutard
Bruno
B
Piotrowski
Yvonne
Y
Hilgenfeld
Rolf
R
Tucker
Paul A
PA
D000246:Adenosine Diphosphate Ribose / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D016765:Coronavirus, Feline / Q000201:enzymology*; D018360:Crystallography, X-Ray; D005994:Glycerophosphates / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
D005994:Glycerophosphates; D017361:Viral Nonstructural Proteins; D000246:Adenosine Diphosphate Ribose; C029620:alpha-glycerophosphoric acid; D012324:RNA-Dependent RNA Polymerase