Early death of Japanese encephalitis virus-infected mice administered a neutralizing cross-reactive monoclonal antibody against glycoprotein E.
In the present study, the effect of two haemagglutination-inhibition (HAI)-negative auto-reactive (NHA-1 and NHA-2) monoclonal antibodies (MAbs) against glycoprotein E (gpE) of Japanese encephalitis virus (JEV) administered 1 day before or 2 days after intracerebral (i.c.) inoculation of JEV was studied in mice. Of the two MAbs that cross-reacted with West Nile virus (WNV) and histones, the first one (NHA-1) neutralized JEV, while the second one was non-neutralizing. NHA-1 MAb given intraperitoneally (i.p.) 1 day before virus infection induced early death by about 2 days in comparison to controls, whereas mice administered HAI-positive anti-gpE JEV specific MAbs (Hs-1 or Hs-4) were invariably protected. In contrast, MAb NHA-2 failed to produce any effect in mice. Since the similar virus titers were recorded in the brains of experimental and control infected mice, the present results indicated a modification of the biological activity of JEV by the pre-existing MAb NHA-1 that might be leading to an early death of mice.


BACKGROUND
Japanese encephalitis virus; neutralizing cross-reactive monoclonal antibody.
Gupta
A K
AK
National Institute of Virology, Indian Council of Medical Research, 20-A, Dr. Ambedkar Road, P.B. No. 11, Pune-411001, India. drashok.gpt@gmail.com
Lad
V J
VJ
Koshy
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D001921:Brain / Q000821:virology; D003429:Cross Reactions; D004664:Encephalitis Virus, Japanese / Q000472:pathogenicity*; D004672:Encephalitis, Japanese / Q000401:mortality; D006385:Hemagglutination Inhibition Tests; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus