The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells.
The differentiation of human embryonic stem cells (hESCs) into cardiomyocytes (CMs) using embryoid bodies (EBs) is relatively inefficient and highly variable. Formation of EBs using standard enzymatic disaggregation techniques results in a wide range of sizes and geometries of EBs. Use of a 3-D cuboidal microwell system to culture hESCs in colonies of defined dimensions, 100-500 microm in lateral dimensions and 120 microm in depth, enabled formation of more uniform-sized EBs. The 300 microm microwells produced highest percentage of contracting EBs, but flow cytometry for myosin light chain 2A (MLC2a) expressing cells revealed a similar percentage (approximately 3%) of cardiomyocytes formed in EBs from 100 microm to 300 microm microwells. These data, and immunolabeling with anti-MF20 and MLC2a, suggest that the smaller EBs are less likely to form contracting EBs, but those contracting EBs are relatively enriched in cardiomyocytes compared to larger EB sizes where CMs make up a proportionately smaller fraction of the total cells. We conclude that microwell-engineered EB size regulates cardiogenesis and can be used for more efficient and reproducible formation of hESC-CMs needed for research and therapeutic applications.
Mohr
Jeffrey C
JC
Department of Chemical and Biological Engineering, University of Wisconsin College of Engineering, 1415 Engineering Drive, Madison, WI 53706, USA. mohr.jeff@gmail.com
Zhang
Jianhua
J
Azarin
Samira M
SM
Soerens
Andrew G
AG
de Pablo
Juan J
JJ
Thomson
James A
JA
Lyons
Gary E
GE
Palecek
Sean P
SP
Kamp
Timothy J
TJ
D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D048429:Cell Size; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D038081:Organogenesis; D013997:Time Factors
D018994:Myosin Light Chains