A candidate gene study of canine joint diseases.
Canine osteoarthritis (OA) commonly occurs in association with articular diseases, such as hip dysplasia (HD), elbow dysplasia (ED), or cranial cruciate ligament rupture (CCLR). We hypothesized that a common genomic risk for the development of canine joint disease and canine OA would be identified by evaluating the allele frequencies of candidate gene single nucleotide polymorphisms (SNPs) in dogs with OA associated with different articular diseases when compared with a general population of breed-matched dogs. DNA was extracted from blood samples obtained from Labrador Retrievers and Golden Retrievers surgically treated for ED, HD, and CCLR and confirmed to have radiographic evidence of OA. One hundred and thirteen SNPs in 20 candidate genes were genotyped. No significant associations were identified for SNPs or haplotypes in the candidate genes for the diseases evaluated. The candidate gene approach for the study of genetic association is unlikely to be successful for complex canine diseases such as OA without prior trait mapping evaluation.
Clements
Dylan N
DN
Division of Veterinary Clinical Sciences, The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, UK. dylan.clements@ed.ac.uk
Short
Andrea D
AD
Barnes
Annette
A
Kennedy
Lorna J
LJ
Ferguson
John F
JF
Butterworth
Steven J
SJ
Fitzpatrick
Noel
N
Pead
Matthew
M
Bennett
David
D
Innes
John F
JF
Carter
Stuart D
SD
Ollier
William E R
WE
D000818:Animals; D004285:Dogs; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D007592:Joint Diseases / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide