Proteome organization in a genome-reduced bacterium.
The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.
Kühner
Sebastian
S
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
van Noort
Vera
V
Betts
Matthew J
MJ
Leo-Macias
Alejandra
A
Batisse
Claire
C
Rode
Michaela
M
Yamada
Takuji
T
Maier
Tobias
T
Bader
Samuel
S
Beltran-Alvarez
Pedro
P
Castaño-Diez
Daniel
D
Chen
Wei-Hua
WH
Devos
Damien
D
Güell
Marc
M
Norambuena
Tomas
T
Racke
Ines
I
Rybin
Vladimir
V
Schmidt
Alexander
A
Yus
Eva
E
Aebersold
Ruedi
R
Herrmann
Richard
R
Böttcher
Bettina
B
Frangakis
Achilleas S
AS
Russell
Robert B
RB
Serrano
Luis
L
Bork
Peer
P
Gavin
Anne-Claude
AC
D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D019295:Computational Biology; D016680:Genome, Bacterial; D013058:Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways; D008854:Microscopy, Electron; D008954:Models, Biological; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000032:analysis* / Q000378:metabolism; D009177:Mycoplasma pneumoniae / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010363:Pattern Recognition, Automated; D025941:Protein Interaction Mapping; D020543:Proteome; D049490:Systems Biology
D001426:Bacterial Proteins; D046912:Multiprotein Complexes; D020543:Proteome