A novel 1,2,4-triazole-based copper(II) complex: synthesis, characterization, magnetic property and nuclease activity.
A novel binuclear copper(II) complex [Cu(2)L(mu-SO(4))](PF(6))(2) (1) (L=3,5-bis (bis(pyridine-2-ylmethyl)amino)methyl)-4H-1,2,4-triazol-4-amine) has been synthesized and structurally characterized. X-ray structure shows that the two copper(II) atoms are bridged by one bidentate sulfate ion and the 1,2,4-triazole ring of L with Cu1cdots, three dots, centeredCu2 distance of 4.404A. Each copper(II) center has a distorted trigonal-bipyramidal configuration. Variable-temperature magnetic susceptibility studies (2-300K) indicate the existence of weak antiferromagnetic coupling between the copper(II) ions in complex 1. The interaction of complex 1 with calf thymus DNA (CT-DNA) has been studied by UV absorption, fluorescence spectroscopy, circular dichroism spectroscopy, viscosity and cyclic voltammetry. Furthermore, complex 1 was able to promote single and double strand DNA cleavage in both aerobic and anaerobic conditions, the pseudo-Michaelis-Menten kinetic parameters k(cat)=2.58h(-1) and K(m)=1.2x10(-4)M were obtained for 1. The hydrolytic cleavage of DNA by the complex was supported by the evidence from free radical quenching, anaerobic experiment, thiobarbituric acid-reactive substances (TBARS) assay.
Li
Dong-Dong
DD
Department of Chemistry, Nankai University, Tianjin 300071, PR China.
Tian
Jin-Lei
JL
Gu
Wen
W
Liu
Xin
X
Yan
Shi-Ping
SP
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008280:Magnetics; D015394:Molecular Structure; D010100:Oxygen / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles / Q000737:chemistry*
D056831:Coordination Complexes; D014230:Triazoles; C045575:1,2,4-triazole; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases; D010100:Oxygen