MALDI Top-Down sequencing: calling N- and C-terminal protein sequences with high confidence and speed.
The ability to match Top-Down protein sequencing (TDS) results by MALDI-TOF to protein sequences by classical protein database searching was evaluated in this work. Resulting from these analyses were the protein identity, the simultaneous assignment of the N- and C-termini and protein sequences of up to 70 residues from either terminus. In combination with de novo sequencing using the MALDI-TDS data, even fusion proteins were assigned and the detailed sequence around the fusion site was elucidated. MALDI-TDS allowed to efficiently match protein sequences quickly and to validate recombinant protein structures-in particular, protein termini-on the level of undigested proteins.
Suckau
Detlev
D
Bruker Daltonics, Bremen, Germany. dsu@bdal.de
Resemann
Anja
A
D000465:Algorithms; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D005656:Fungal Proteins / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D020539:Sequence Analysis, Protein / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D005656:Fungal Proteins; D011506:Proteins; D011994:Recombinant Proteins