[Genetic characterization of enterovirus 71 complete genome isolated in Beijing, 2008].
OBJECTIVE
To investigate the characterization of the complete genome of EV71 in Beijing, 2008 and to provide basis for selecting appropriate virus strain to develop vaccine.


METHODS
12 throat swab samples were collected from children with hand-foot-mouth disease (HFMD). One sample named 08YM-3 was cultured and isolated in vero cells. Viral RNA was extracted and carried out by RT-PCR and 5', 3' rapid amplification of cDNA ends (RACE) to obtain the sequence from 08YM-3. PCR products were cloned and analyzed. Nucleotide identity between sequences was calculated and sequence alignments were made to generate phylogenetic trees using MegAlign in DNAStar.


RESULTS
3 clones were constructed that covered EV71 complete genome. Data from sequences analysis showed that this viral strain named BJ08 shared 95.6%-96.7%, 88.3%-96.1%, 78.1%-94.0%, 90.8%-94.6%, 85.9%-94.1% and 90.9%-93.9% in 5' UTR, P1, P2, P3, 3' UTR region and complete genome with C4 subtype, respectively. BJ08 showed low nucleotides identity (<90%) with other subtypes. Phylogenetic trees established from alignment of the complete genome and VP1 region indicated that BJ08 belonged to C4 subtype. BJ08 and C4 subtype strains shared the same amino acids in 6 sites in VP1 region, which were associated with EV71 subtype. There was no mutation in VP1 antigen epitope (92-107aa).


CONCLUSIONS
This BJ08 strain belonged to C4 subtype. Further study on EV71 complete genome would have great significance for vaccine research.
Yao
Xin
X
National Institute for Control of Pharmaceutical and Biological Products, Beijing, China.
Mao
Qun-Ying
QY
Huang
Wei-Jin
WJ
He
Peng
P
Zhou
Cheng
C
Zhang
Wei
W
Liang
Zheng-Lun
ZL
Li
Feng-Xiang
FX
Wang
Jun-Zhi
JZ
D000818:Animals; D001483:Base Sequence; D002648:Child; D002681:China; D002522:Chlorocebus aethiops; D029821:Enterovirus A, Human / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells
D012367:RNA, Viral