title: Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies.
abstract: BACKGROUND
  An excessive inflammatory response is thought to account for the pathogenesis of sepsis and multiple organ dysfunction syndrome (MODS) after severe trauma. The interleukin-10 (IL-10) is a potent anti-inflammatory cytokine. The objectives of this prospective study were to investigate the distribution of IL-10 promoter polymorphisms in a cohort of 308 Chinese Han patients with major trauma, and to identify associations of IL-10 promoter polymorphisms with IL-10 production and incidence of sepsis and MODS.
  
  
  METHODS
  A total of 308 patients with major trauma were included in this study. The genotypes of polymorphisms -1082, -819 and -592 were determined by polymerase chain reaction-restriction fragment length polymorphism. The IL-10 levels in the supernatants were determined with enzyme-linked immunoabsorbent assay.
  
  
  RESULTS
  The -1082A and -592A alleles were significantly associated with lower lipopolysaccharide-induced IL-10 production in an allele-dose dependent fashion. There was no significant difference for the -819 polymorphism. Except for the -1082 polymorphism, the -819 and -592 polymorphisms were not significantly associated with sepsis morbidity rate and MOD scores.
  
  
  CONCLUSIONS
  Our results further confirm the functionality of the IL-10 promoter single nucleotide polymorphisms in relation to IL-10 production. They also suggest that individual difference in IL-10 production in trauma patients might be at least in part related to genetic variations in the IL-10 promoter region.
authors: State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Yuzhong District, Chongqing, 400042, PR China. zengling_1025@126.com
authors: Ling
authors: L
authors: Zeng
authors: Wei
authors: W
authors: Gu
authors: Kehong
authors: K
authors: Chen
authors: Dongpo
authors: D
authors: Jiang
authors: Lianyang
authors: L
authors: Zhang
authors: Dingyuan
authors: D
authors: Du
authors: Ping
authors: P
authors: Hu
authors: Qing
authors: Q
authors: Liu
authors: Suna
authors: S
authors: Huang
authors: Jianxin
authors: J
authors: Jiang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D015599:Trauma Severity Indices; D014947:Wounds and Injuries / Q000235:genetics*; D055815:Young Adult
chemical_list: D016753:Interleukin-10