Actions of redox-active compound resveratrol under hydrogen peroxide insult in C6 astroglial cells.
The mechanisms by which resveratrol (3,5,4'-trihydroxy-stilbene) imparts neural effects is not well understood. We previously demonstrated that, depending upon the concentration of resveratrol and the cell type, this compound exerts anti-or pro-oxidant effects. In the present study, we investigated the effects of resveratrol on H(2)O(2)-mediated genotoxicity in C6 astroglial cells (I - 1mM H(2)O(2)/30 min or II - 0.1mM H(2)O(2)/6h), evaluated by micronucleus assay, lipid peroxidation (TBARS) and membrane integrity. H(2)O(2) increased micronuclei to 1.5 (I) and 1.7-fold (II), compared to control cells. This DNA damage was prevented (I) or partially prevented (II) by resveratrol. Oxidative insult also increased TBARS, 52% in I and 38% in II, P<0.05. These effects were prevented by resveratrol in I and increased in II (70% of increase). Present data contribute to the understanding of resveratrol effects under oxidative stress damage.
Quincozes-Santos
André
A
Research Group in Neuroglial Plasticity, Department of Biochemistry, Institute of Health's Basic Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. andrequincozes@yahoo.com.br
Andreazza
Ana Cristina
AC
Gonçalves
Carlos-Alberto
CA
Gottfried
Carmem
C
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004249:DNA Damage; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D015162:Micronucleus Tests; D016877:Oxidants / Q000633:toxicity*; D010084:Oxidation-Reduction; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013638:Tars / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D000975:Antioxidants; D004396:Coloring Agents; D008563:Membrane Lipids; D016877:Oxidants; D013267:Stilbenes; D013638:Tars; D013778:Tetrazolium Salts; D013844:Thiazoles; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D001205:Ascorbic Acid; D000077185:Resveratrol