[Using protein chips to explore the differential expression of serum proteins in healthy women and OEC patients].
OBJECTIVE
To explore the differentially expressed serum proteins in healthy women and OEC patients by using protein chips.


METHODS
Sera of 11 cases of EOC and 11 matched healthy women were labeled with cy3 and cy5 fluorescent dyes and then were made to hybridized with the same protein antibody chips. The internally normalized ratio (INR) was calculated according to the fluorescent intensity of each protein spot. The value of INR > 2.0 or < 0.7 was considered as a cut-value to screen the differentially expressed serum proteins in EOC patients and healthy women.


RESULTS
Twenty-seven differentially expressed serum proteins were found in healthy women and EOC patients, including 17 up-regulated and 10 down-regulated proteins in EOC patients.


CONCLUSIONS
Proteome-protein antibody chip can be used to detect the differentially expressed serum proteins in the tissues. These target proteins will facilitate the study of diagnostic biomarkers of EOC and aid in monitoring the therapeutic effect of EOC.
Chen
Fei
F
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing.
Yang
Jia-xin
JX
Wu
Ming
M
Shen
Keng
K
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood*; D040081:Protein Array Analysis / Q000379:methods*
D001798:Blood Proteins