Molecular cloning and expression of a 2-Cys peroxiredoxin gene in the crustacean Eurypanopeus depressus induced by acute hypo-osmotic stress.
Peroxiredoxins (Prxs) are a family of ubiquitous proteins that help minimize the harmful effects of oxidative stress by catalyzing the reduction of hydrogen peroxide (H2O2) and organic hydroperoxides to less harmful forms. A full-length cDNA corresponding to a 2-Cys Prx gene was isolated from the flatback mud crab Eurypanopeus depressus and designated as EdPrx-1 (GenBank accession no. EU684547). EdPrx-1 has a major open-reading frame of 594 bp and is capable of encoding a polypeptide of 198 amino acid residues. Like other 2-Cys Prxs, EdPrx-1 protein possesses two conserved cysteine residues that play an essential role for the antioxidant activity of the proteins. The EdPrx-1 protein, as deduced from the cDNA sequence, shows a high level (74-93%) of sequence similarity to the 2-Cys Prxs from other crustaceans as well as those from many arthropod species (73-76% similarity). It shares about 70% sequence similarity with homologs from mammalian species. EdPrx-1 gene is expressed at low level in the gill, hypodermis, and hepatopancreas tissues of the crab under non-stressed condition; however, its expression is elevated about three-fold in the gills under hypo-osmotic stress. This suggests a possible role in protecting against oxidative stress caused by the increased metabolic activities associated with hyperosmoregulation.
Van Horn
Julie
J
Department of Marine and Ecological Sciences, Coastal Watershed Institute, Florida Gulf Coast University, Fort Myers, FL 33965, USA.
Malhoe
Vedanta
V
Delvina
Marlin
M
Thies
Megan
M
Tolley
S Gregory
SG
Ueda
Takashi
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D051381:Rats; D054712:Salinity; D013312:Stress, Physiological / Q000235:genetics*; D015996:Survival Rate
D018076:DNA, Complementary; D054464:Peroxiredoxins