Metabonomic phenotype and identification of "heart blood stasis obstruction pattern" and "qi and yin deficiency pattern" of myocardial ischemia rat models.
The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up- or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".
Yan
Bei
B
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
A
JiYe
J
Hao
HaiPing
H
Wang
GuangJi
G
Zhu
XuanXuan
X
Zha
WeiBin
W
Liu
LinSheng
L
Guan
EnZe
E
Zhang
Ying
Y
Gu
ShengHua
S
Huang
Qing
Q
Zheng
YuanTing
Y
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D055442:Metabolome / Q000502:physiology; D055432:Metabolomics / Q000379:methods*; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D010641:Phenotype; D019413:Qi; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016710:Yin Deficiency / Q000378:metabolism* / Q000473:pathology
D000596:Amino Acids; D015415:Biomarkers