Pattern of expression of apoptosis and inflammatory genes in humans exposed to arsenic and/or fluoride.
We have assessed whether the combined exposure to arsenic (As) and fluoride (F) exerts a different effect than the exposure to As alone on the pattern of expression of apoptosis and inflammatory genes by immune cells. RNA was extracted from peripheral blood mononuclear cells from twenty individuals exposed or not to As or F or both. Then, cDNA was isolated, and the expression of 180 genes related to apoptosis and inflammation was tested by a cDNA array test. We found significant differences in the expression of 9 apoptosis and 15 inflammation genes in the three exposed groups compared to non-exposed individuals. In addition, subjects exposed to As or F or both showed different patterns of expression of at least 19 genes. Our data indicate that the combined exposure to As and F has a different effect on gene expression than the exposure to As or F alone.
Salgado-Bustamante
Mariana
M
Department of Immunology, Facultad de Medicina, UASLP, Mexico.
Ortiz-Pérez
María D
MD
Calderón-Aranda
Emma
E
Estrada-Capetillo
Lizbeth
L
Niño-Moreno
Perla
P
González-Amaro
Roberto
R
Portales-Pérez
Diana
D
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001152:Arsenicals / Q000009:adverse effects* / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D005459:Fluorides / Q000009:adverse effects* / Q000032:analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014881:Water Supply / Q000032:analysis; D055815:Young Adult
D001152:Arsenicals; D012333:RNA, Messenger; D012313:RNA; D005459:Fluorides