The cancer/testis antigen CAGE induces MMP-2 through the activation of NF-kappaB and AP-1.
Cancer-associated antigen (CAGE) induces the expression of matrix metalloproteinase-2 (MMP-2) by activating Akt, which in turn interacts with inhibitory kappa kinase beta (IkappaKbeta) to activate nuclear factor kappaB (NF-kappaB). Akt and p38 mitogen activated protein kinase (p38 MAPK) are necessary for CAGE-mediated induction of the AP-1 subunit JunB, whereas extracellular regulated kinase (ERK) is necessary for the induction of fos-related antigen-1 (Fra-1). Induction of MMP-2 by CAGE requires activator of protein-1 (AP-1) to be bound. Specific binding of JunB to MMP-2 promoter sequences was shown by chromatin immunoprecipitation (ChIP) analysis.
Kim
Youngmi
Y
School of Biological Sciences, College of Natural Sciences, Kangwon National University, Chunchon, Korea.
Jeoung
Dooil
D
D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers; D024202:Electrophoretic Mobility Shift Assay; D004789:Enzyme Activation; D004790:Enzyme Induction; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D000951:Antigens, Neoplasm; D017931:DNA Primers; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D011494:Protein Kinases; D020778:Matrix Metalloproteinase 2