title: First identification of Neospora caninum by PCR in aborted bovine foetuses in Romania.
abstract: The first identification of Neospora caninum infection in aborted bovine foetuses in Romania is reported. Nine aborted foetuses were collected from a dairy farm. The foetal age of foetuses was between 3 and 7 months. A 7-month-old foetus was mummified. N. caninum DNA Nc-5 region was amplified from samples extracted from brain tissues of three (33.3%) aborted foetuses. The foetal ages of PCR positive foetuses were 3, 4 and 7 months (mummified). No specific lesions or tissue cysts were found to the histological examination, because of advanced autolysis of brains.
authors: Department of Parasitology and Parasitic Diseases, University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Mănăştur Street, Cluj Napoca, Romania.
authors: Ovidiu
authors: O
authors: Suteu
authors: Adriana
authors: A
authors: Titilincu
authors: David
authors: D
authors: Modrý
authors: Andrei
authors: A
authors: Mihalca
authors: Viorica
authors: V
authors: Mircean
authors: Vasile
authors: V
authors: Cozma
mesh_terms: D037881:Aborted Fetus / Q000469:parasitology*; D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012383:Romania
chemical_list: D016054:DNA, Protozoan