Role of RNA structure in regulating pre-mRNA splicing.
Pre-mRNA splicing involves removing non-coding introns from RNA transcripts. It is carried out by the spliceosome, along with other auxiliary factors. In general, research in splicing has focused on the sequences within the pre-mRNA, without considering the structures that these sequences might form. We propose that the role of RNA structure deserves more consideration when thinking about splicing mechanisms. RNA structures can inhibit or aid binding of spliceosomal components to the pre-mRNA, or can increase splicing efficiency by bringing important sequences into close proximity. Recent reports have identified proteins and small molecules that can regulate splicing by modulating RNA structures, thereby expanding our knowledge of the mechanisms used to regulate splicing.
Warf
M Bryan
MB
Institute of Molecular Biology, and Department of Chemistry, University of Oregon, Eugene, Oregon 97403, USA.
Berglund
J Andrew
JA
D000818:Animals; D001483:Base Sequence; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing
D012322:RNA Precursors; D012313:RNA