Comparison of database search strategies for high precursor mass accuracy MS/MS data.
In shotgun proteomics, the analysis of tandem mass spectrometry data from peptides can benefit greatly from high mass accuracy measurements. In this study, we have evaluated two database search strategies which use high mass accuracy measurements of the peptide precursor ion. Our results indicate that peptide identifications are improved when spectra are searched with a wide mass tolerance window and precursor mass is used as a filter to discard incorrect matches. Database searches with a peptide data set constrained to peptides within a narrow mass window resulted in fewer peptide identifications but a significantly faster database search time.
Hsieh
Edward J
EJ
Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA.
Hoopmann
Michael R
MR
MacLean
Brendan
B
MacCoss
Michael J
MJ
D002853:Chromatography, Liquid; D030562:Databases, Protein; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D029701:Saccharomyces cerevisiae Proteins