On the effects of UV-C and pH on the mechanical behavior, molecular conformation and cell viability of collagen-based scaffold for vascular tissue engineering.
Collagen-based vascular substitutes represent in VTE a valid alternative for the replacement of diseased small-calibre blood vessels. In this study, collagen gel-based scaffolds were crosslinked combining modulation of pH and UV-C radiation. The effects on the mechanical properties, on the molecular structure and on cell viability and morphology were investigated. The mechanical response increased as a function of pH or UV-C dose and strongly depended on the test speed. Collagen molecular conformation resulted only slightly modified. While cell adhesion was not significantly altered, cell proliferation partially decreased in function of pH and UV-C. These findings suggest that UV-C treated collagen gels can represent an adequate substrate for VTE applications.
Achilli
Matteo
M
Laboratory for Biomaterials and Bioengineering, Department of Materials Engineering & Research Center, Quebec University Hospital, Laval University, Quebec City, G1K 7P4, Canada.
Lagueux
Jean
J
Mantovani
Diego
D
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003094:Collagen / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration / Q000187:drug effects / Q000528:radiation effects; D055595:Mechanical Phenomena / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008968:Molecular Conformation; D041681:NIH 3T3 Cells; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
D003094:Collagen