Studying the cytotoxicity and oxidative stress induced by two kinds of bentonite particles on human B lymphoblast cells in vitro.
The aim of the present study was to evaluate the cytotoxicity and oxidative stress induced by native and active bentonite particles (BPs) on human B lymphoblast cells using seven assays. Our results showed that the order of cytotoxicity was: active BPs>native BPs>quartz particles (DQ-12)>gypsum, according to the IC50 values in CCK-8 assay and neutral red uptake (NRU) assay. The lactate dehydrogenase (LDH) leakage, the proportions of early apoptotic cells, the reactive oxygen species (ROS) generation, the superoxide dismutase (SOD) inhibition and the malondialdehyde (MDA) release in the native and active BPs groups were significantly higher than those in the gypsum and DQ-12 groups (P<0.05 or P<0.01). Moreover, the cytotoxicity of active BPs with higher adsorption capacity of phenol was higher than that of native BPs with relatively lower adsorption capacity of phenol. The oxidative stress induced by active BPs was significantly higher than that induced by native BPs (P<0.05 or P<0.01). The water-soluble fractions of BPs did not induce the cytotoxicity and ROS generation. These findings indicated that active and native BPs could induce significantly the cytotoxic effects and oxidative stress on human B lymphoblast cells in vitro. The cytotoxic difference between active BPs and native BPs may be associated with the adsorption capacity of BPs and oxidative stress induced by BPs to a certain extent. The insoluble particle fractions may play a main role in the cytotoxic effects and oxidative stress induced by BPs.
Zhang
Meibian
M
Zhejiang University, Medical College, Institute of Environmental Medicine, Hangzhou, Zhejiang, People's Republic of China.
Lu
Yezhen
Y
Li
Xiaoxue
X
Chen
Qing
Q
Lu
Longxi
L
Xing
Mingluan
M
Zou
Hua
H
He
Jiliang
J
D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D001546:Bentonite / Q000633:toxicity*; D002133:Calcium Sulfate / Q000633:toxicity; D002460:Cell Line; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009499:Neutral Red / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011791:Quartz / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D001546:Bentonite; D011791:Quartz; D009499:Neutral Red; D008315:Malondialdehyde; D045022:Lactate Dehydrogenases; D013482:Superoxide Dismutase; D002133:Calcium Sulfate