title: Distribution and expression pattern of claudins 6, 7, and 9 in diffuse- and intestinal-type gastric adenocarcinomas.
abstract: BACKGROUND
  Intestinal- and diffuse-type gastric adenocarcinomas differ in clinical outcome and genetic profile. Abnormal claudin expression has been well documented in several malignancies. Our aim was to find specific claudin markers for each type.
  
  
  METHODS
  Fifty paraffin-embedded tissue blocks of diffuse- and intestinal-type gastric adenocarcinomas and fresh gastric biopsies obtained endoscopically from 20 patients with a presumptive diagnosis of gastric cancer were analyzed. Claudin-specific polyclonal and monoclonal antibodies were used for immunohistochemistry and Western blot analysis in total lysate and subcellular fractions.
  
  
  RESULTS
  Claudin-6 expression was high in both types. Claudin-7 was expressed mainly in the diffuse-type whereas claudin-9 was mainly found in the apical membrane of the gland cells in the intestinal-type. Strong claudin-9 expression was associated with higher mortality rate (66%) in the diffuse type vs the intestinal type (25%) after a 2-year follow-up.
  
  
  CONCLUSIONS
  Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis, and their detection is a useful prognostic marker in "intestinal-" and "diffuse-type" gastric adenocarcinomas.
authors: Depto Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF, México.
authors: Erika
authors: E
authors: Rendón-Huerta
authors: Fortoul
authors: F
authors: Teresa
authors: Gorráez María
authors: GM
authors: Teresa
authors: Garcia-Samper
authors: GS
authors: Xochitl
authors: Alvarez-Fernández
authors: AF
authors: Georgina
authors: Zavala-Zendejas
authors: ZZ
authors: Veronica
authors: Luis Felipe
authors: LF
authors: Montaño
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D057167:Claudins; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C473255:CLDN7 protein, human; C524757:CLDN9 protein, human; D057167:Claudins; D008565:Membrane Proteins; C122101:claudin 6