Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
Hong
JiYoung
J
Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea.
Kang
Byunghak
B
Kim
Ahyoun
A
Hwang
Seoyoun
S
Lee
Sunwha
S
Kim
Jonghyen
J
Lee
Hyun-Young
HY
Kang
Sang-Hyeon
SH
Cheon
Doo-Sung
DS
D017931:DNA Primers / Q000235:genetics*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers