Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay.
OBJECTIVE
The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1.


METHODS
Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level.


RESULTS
Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61% of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1% caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1% extract.


CONCLUSIONS
The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.
Correa
Gabriela Tavares Bekner
GT
Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil.
Veranio
Gabriel Alves Costa
GA
Silva
Licínio Esmaeraldo
LE
Hirata Junior
Raphael
R
Coil
Jeffry M
JM
Scelza
Miriam F Zaccaro
MF
D001466:Barium Sulfate / Q000633:toxicity; D001729:Bismuth / Q000633:toxicity; D001881:Borates / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity*; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005054:Eugenol / Q000633:toxicity; D006801:Humans; D008422:Materials Testing; D009000:Monocytes / Q000187:drug effects; D012117:Resins, Synthetic / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D014343:Trypan Blue; D015034:Zinc Oxide / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
D001881:Borates; D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C534916:epoxy resin-based root canal sealer; D001466:Barium Sulfate; D005054:Eugenol; C038425:Fillcanal; D014343:Trypan Blue; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D001729:Bismuth