title: [Determination of forsythoside A in fructus forsythiae by NIR].
abstract: OBJECTIVE
  Determine the content of forsythoside A in fructus forsythiae by combining near-infrared spectroscopy with the chemometrics method.
  
  
  METHODS
  HPLC was used as a reference method to determine the content of forsythoside A in fructus forsythiae. Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method.
  
  
  RESULTS
  RMSECV (root-mean-square error of cross-validation) of the model for forsythoside A was 0.221 and the correlation coefficients of the calibration model was 0.969. External validation with external validation samples proved that the relative coefficient of the predicted value and the HPLC value was 0.961 and the RMSEP was 0.18.
  
  
  CONCLUSIONS
  NIRS can be used in the determination of forsythoside A, which sets up the foundation of product-line control of fructus forsythiae.
authors: Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. kingstar1016@sina.com
authors: Xing
authors: X
authors: Wang
authors: Yan
authors: Y
authors: Bai
authors: Zhihong
authors: Z
authors: Chen
authors: Haiyan
authors: H
authors: Gong
authors: Wei
authors: W
authors: Zhang
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry; D031657:Forsythia / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; C058049:forsythiaside