Ellagic acid inhibits lipopolysaccharide-induced expression of enzymes involved in the synthesis of prostaglandin E2 in human monocytes.
Ellagic acid, a natural polyphenol found in certain fruits, nuts and vegetables, has in recent years been the subject of intense research within the fields of cancer and inflammation. Pain, fever and swelling, all typical symptoms of inflammation, are ascribed to elevated levels of PGE2. In the present study, we have investigated the effects of ellagic acid on PGE2 release and on prostaglandin-synthesising enzymes in human monocytes. Ellagic acid was found to inhibit Ca ionophore A23187-, phorbol myristate acetate- and opsonised zymosan-induced release of PGE2 from monocytes pre-treated with the inflammatory agent lipopolysaccharide. Ellagic acid suppressed the lipopolysaccharide-induced increase in protein expression of cyclo-oxygenase-2 (COX-2), microsomal PGE synthase-1 (mPGEs-1) and cytosolic phospholipase A2alpha (cPLA2alpha), while it had no effect on the constitutively expressed COX-1 protein. Ellagic acid had no apparent inhibitory effect on these enzymes when the activities were determined in cell-free assays. We conclude that the inhibitory effect of ellagic acid on PGE2 release from monocytes is due to a suppressed expression of COX-2, mPGEs-1 and cPLA2alpha, rather than a direct effect on the activities of these enzymes.
Karlsson
Sofia
S
Department of Chemistry and Biomedical Sciences, Karlstad University, Karlstad, Sweden.
Nånberg
Eewa
E
Fjaeraa
Christina
C
Wijkander
Jonny
J
D000001:Calcimycin / Q000037:antagonists & inhibitors; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000097:blood / Q000378:metabolism*; D004610:Ellagic Acid / Q000494:pharmacology*; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008861:Microsomes / Q000201:enzymology; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology*; D054467:Phospholipases A2 / Q000378:metabolism; D000071876:Prostaglandin-E Synthases; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors
D008070:Lipopolysaccharides; D004610:Ellagic Acid; D000001:Calcimycin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D054467:Phospholipases A2; D019746:Intramolecular Oxidoreductases; C000605862:PTGES protein, human; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate