title: Single channel exact 3-D blind image deconvolution from cylindrically symmetric blur kernel.
abstract: The rotational symmetry of point spread function (PSF) is common for many imaging systems such as optical microscopes, cameras, astigmatism corrected electron microscopes, and etc. In 2-D deconvolution problem, we showed that an exact PSF estimation from a single measured image on a flat background is possible by exploiting the circular symmetry. This paper extends the idea to 3-D blind deconvolution problem. More specifically, we show that the exact recovery of 3-D PSF is possible from a single set of z-stack images if the PSF of the microscope has a cylindrical symmetry and there exists enough working distance to cover the volumetric sample from top to bottom. The resulting algorithm allows an accurate computational optical sectioning of biological specimen from z-stack images using brightfield or fluorescence microscopes without separate PSF measurements. Experimental results confirm our theory.
authors: Korea Advanced Institute of Science and Technology, Dept. of Bio and Brain Engineering, 373-1 Guseong-dong, Yuseong-gu, Daejeon, Republic of Korea.
authors: Kwang Eun
authors: KE
authors: Jang
authors: Jong Chul
authors: JC
authors: Ye
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D008962:Models, Theoretical