title: A feasibility study of adenosine triphosphate-based chemotherapy response assay (ATP-CRA) as a chemosensitivity test for lung cancer.
abstract: OBJECTIVE
  A chemosensitivity test can reflect the differences in responses of individual cancer patients to chemotherapeutic agents. The adenosine triphosphate-based chemotherapy response assay (ATP-CRA) is an accurate method, which does not require a large amount of tissue specimen. So far, no studies have evaluated the utility of the ATP-CRA in Korea. Therefore, we investigated the clinical usefulness of the ATP-CRA in 53 patients with lung cancer.
  
  
  METHODS
  Tumor tissues were obtained from bronchoscopic biopsies or surgical resections. The validity of ATP-CRA was assessed focusing on the success rate, experimental error level (intraassay mean coefficient of variation [CV]) and reproducibility.
  
  
  RESULTS
  The overall success rate of ATP-CRA was 90.6% (48/53). Normal cells were effectively eliminated from the tumor tissues with the use of ficoll gradient centrifugation and immunomagnetic separation, which was confirmed using loss of heterozygosity analysis of the 3p deletion. The mean CV of ATP assays was 10.5+/-4.6%. The reproducibility of ATP assays was 94+/-3.8%. The results of the ATP assays were reported to physicians within 7 days of specimen collection. More than 6 anticancer drugs were tested on the tumor specimens obtained from bronchoscopic biopsies.
  
  
  CONCLUSIONS
  The ATP-CRA is a stable, accurate and potentially practical chemosensitivity test in patients with lung cancer.
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors: Shin Myung
authors: SM
authors: Kang
authors: Moo Suk
authors: MS
authors: Park
authors: Joon
authors: J
authors: Chang
authors: Se Kyu
authors: SK
authors: Kim
authors: Haeryoung
authors: H
authors: Kim
authors: Dong-Hwan
authors: DH
authors: Shin
authors: Kyung Young
authors: KY
authors: Chung
authors: Dae Joon
authors: DJ
authors: Kim
authors: Joo Hyuk
authors: JH
authors: Sohn
authors: Sung Ho
authors: SH
authors: Choi
authors: Jeongmi
authors: J
authors: Kim
authors: Eun Jin
authors: EJ
authors: Yoon
authors: Joo-Hang
authors: JH
authors: Kim