Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats.
Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [(14)C]proline (Pro group) or glycyl-[(14)C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolyl-hydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.
Watanabe-Kamiyama
Mari
M
Laboratory of Nutrition, Department of Science of Food Function and Health, Graduate School of Agricultural Science, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan.
Shimizu
Muneshige
M
Kamiyama
Shin
S
Taguchi
Yasuki
Y
Sone
Hideyuki
H
Morimatsu
Fumiki
F
Shirakawa
Hitoshi
H
Furukawa
Yuji
Y
Komai
Michio
M
D000042:Absorption; D000284:Administration, Oral; D000818:Animals; D002645:Chickens; D003094:Collagen / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D010024:Osteoporosis / Q000188:drug therapy* / Q000378:metabolism; D010052:Ovariectomy; D011492:Protein Hydrolysates / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D011492:Protein Hydrolysates; D003094:Collagen