[Mechanism of hedysari polysaccharide in the apoptosis of human hepatocellular carcinoma HEP-G2 cells].
OBJECTIVE
To study the effects of Hedysari polysaccharide (HPS) on the proliferation and apoptosis of human hepatocellular carcinoma HEP-G2 cells and its mechanism.


METHODS
MTT assay, flow cytometry and fluorescence microscopy were used to the effects of HPS in anti-proliferation, cell cycle arresting and apoptosis induction. Flow cytometry was also used to detect the effects detect of HPS on protein expressions of Bcl-2 and Bax.


RESULTS
HPS could cause apparent inhibition on cell growth, induce G2/M phase arrest and apoptosis on HEP-G2 cells. HPS could down-regulate bcl-2 protein expression and up-regulate Bax protein expression.


CONCLUSIONS
HPS has anti-tumor effects, maybe by the way of inhibiting the cell growth, arresting the G2/M phase, inducing apoptosis, down-regulating bcl-2 and up-regulating Bax protein expression.
Li
Shi-Gang
SG
Medical College, China Three Gorges University, Yichang 443002, China.
Zhang
Yong-Qi
YQ
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein