Antinociceptive property of Emblica officinalis Gaertn (Amla) in high fat diet-fed/low dose streptozotocin induced diabetic neuropathy in rats.
Diabetic neuropathic pain is an important microvascular complication in diabetes mellitus and oxidative stress plays a vital role in associated neural and vascular complications. The present study investigated flavonoid rich fruit extract (ethyl acetate:methanol fraction) of E. officinalis (10 mg/kg), in type II diabetes (high fat diet fed/low dose streptozotocin) induced diabetic neuropathy in male Sprague-Dawley rats. Diabetic rats exhibited a significant hyperalgesia (nociception) as compared to control rats. Treatment with E. officinalis extract (EOE) and quercetin in diabetic rats showed significant increase in tail flick latency in hot immersion test and pain threshold level in hot plate test compared to control rats. The changes in lipid peroxidation status and anti-oxidant enzymes (superoxide dismutase and catalase) levels observed in diabetic rats were significantly restored by E. officinalis extract and quercetin treatment. Both, E. officinalis extract and quercetin attenuated diabetic induced axonal degeneration. The study provides experimental evidence of the preventive and curative effect of E. officinalis on nerve function and oxidative stress in animal model of diabetic neuropathy. Since, E. officinalis fruit is already in clinical use for diabetic patients it may be evaluated for preventive therapy in diabetic patients at risk of developing neuropathy.
Kumar
N Prem
NP
Department of Pharmacology, Krupanidhi College of Pharmacy, # 5, Sarjapur road, Koramangala, Bangalore 560 034, India. premkrupanidhi@yahoo.co.in
Annamalai
A R
AR
Thakur
R S
RS
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D001786:Blood Glucose / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy*; D003929:Diabetic Neuropathies / Q000139:chemically induced / Q000188:drug therapy*; D004041:Dietary Fats / Q000008:administration & dosage*; D005419:Flavonoids / Q000378:metabolism; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010146:Pain / Q000188:drug therapy*; D029842:Phyllanthus emblica / Q000737:chemistry*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D000700:Analgesics; D000903:Antibiotics, Antineoplastic; D001786:Blood Glucose; D004041:Dietary Fats; D005419:Flavonoids; D013311:Streptozocin; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase