Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker.
Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C. cellulovorans that has been cultured on different carbon sources, such as Avicel, xylan, AXP (Avicel-xylan-pectin, 3:1:1) and cellobiose, was explored by two-dimensional gel electrophoresis. To identify the cellulosomal enzymes, we constructed a biomarker using cohesin 6, one of the CbpA cohesins, that was labeled with fluorescence. The major apparent spots were isolated and identified by ESI MS/MS protein sequencing. Fluorescently labeled cohesin clearly showed that the amount of the cellulosomal enzymes was influenced by the available carbon source. EngE, ExgS, EngK, XynB and ManA were most frequently expressed under all conditions. However, EngY was only observed on the AXP culture. We found two novel putative cellulosomal proteins, NC1[GH9] and NC2[GH26], and five unknown proteins, NU1, NU2, NU3, NU4 and NU5. The cohesin biomarker clearly showed different production patterns of the cellulosomal subunits under different culture conditions and revealed novel cellulosomal subunits.
Cho
Woojae
W
School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Jeon
Sang Duck
SD
Shim
Hyun Jung
HJ
Doi
Roy H
RH
Han
Sung Ok
SO
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D044603:Cellulosomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D046974:Clostridium cellulovorans / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000302:isolation & purification; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D000097722:Cohesins
D001426:Bacterial Proteins; D015415:Biomarkers; D002244:Carbon; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D010455:Peptides; D011994:Recombinant Proteins; D000097722:Cohesins