title: Proteome of Salmonella Enterica Serotype Typhimurium Grown in a Low Mg/pH Medium.
abstract: To determine the impact of a low Mg(2+)/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O(2) level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.
authors: Pacific Northwest National Laboratory, Richland, Washington 99352.
authors: Liang
authors: L
authors: Shi
authors: Charles
authors: C
authors: Ansong
authors: Heather
authors: H
authors: Smallwood
authors: Leah
authors: L
authors: Rommereim
authors: Jason E
authors: JE
authors: McDermott
authors: Heather M
authors: HM
authors: Brewer
authors: Angela D
authors: AD
authors: Norbeck
authors: Ronald C
authors: RC
authors: Taylor
authors: Jean K
authors: JK
authors: Gustin
authors: Fred
authors: F
authors: Heffron
authors: Richard D
authors: RD
authors: Smith
authors: Joshua N
authors: JN
authors: Adkins