[Simultaneous determination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC].
OBJECTIVE
To develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.


METHODS
The separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.


RESULTS
The linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.


CONCLUSIONS
The method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.
Liang
Taigang
T
School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
Liu
Enli
E
Zhao
Chengxiao
C
Ban
Shurong
S
Li
Qingshan
Q
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D027845:Malus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids