title: Early replication in pulmonary B cells after infection with Marek's disease herpesvirus by the respiratory route.
abstract: Abstract Natural infection with Marek's disease virus occurs through the respiratory mucosa after chickens inhale dander shed from infected chickens. The early events in the lung following exposure to the feather and squamous epithelial cell debris containing the viral particles remain unclear. In order to elucidate the virological and immunological consequences of MDV infection for the respiratory tract, chickens were infected by intratracheal administration of infective dander. Differences between susceptible and resistant chickens were immediately apparent, with delayed viral replication and earlier onset of interferon (IFN)-gamma production in the latter. CD4(+) and CD8(+) T cells surrounded infected cells in the lung. Although viral replication was evident in macrophages, pulmonary B cells were the main target cell type in susceptible chickens following intratracheal infection with MDV. In accordance, depletion of B cells curtailed viremia and substantially affected pathogenesis in susceptible chickens. Together the data described here demonstrate the role of pulmonary B cells as the primary and predominant target cells and their importance for MDV pathogenesis.
authors: Institute for Animal Health, Compton, Newbury, Berkshire, UK. bbaaten@burnham.org
authors: B J G
authors: BJ
authors: Baaten
authors: K A
authors: KA
authors: Staines
authors: L P
authors: LP
authors: Smith
authors: H
authors: H
authors: Skinner
authors: T F
authors: TF
authors: Davison
authors: C
authors: C
authors: Butter
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens / Q000276:immunology / Q000821:virology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D005241:Feathers / Q000821:virology; D005260:Female; D020022:Genetic Predisposition to Disease; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification / Q000502:physiology*; D007327:Insufflation; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008212:Lymphocyte Depletion; D016131:Lymphocyte Subsets / Q000276:immunology; D008380:Marek Disease / Q000276:immunology / Q000473:pathology / Q000821:virology*; D012867:Skin / Q000821:virology; D013154:Spleen / Q000276:immunology / Q000473:pathology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014132:Trachea; D019562:Viral Load; D014779:Virus Replication
chemical_list: D016207:Cytokines; D004279:DNA, Viral