title: Analysis of somatic and salivary gland antigens of third stage larvae of Rhinoestrus spp. (Diptera, Oestridae).
abstract: Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays performed using pharyngeal swabs. The present study was carried out to characterize larval somatic proteins and salivary glands of Rhinoestrus spp. in a preliminary assessment towards the immunodiagnosis of equine rhinoestrosis. Out of the 212 necropsied horses 13 were positive for the presence of Rhinoestrus spp. larvae. The analysis of the sera from the infected animals by Western blotting assay showed the presence of a specific host humoral immune response against Rhinoestrus spp. larvae and proved that the salivary glands are the major immunogens in horse nasal botflies.
authors: Department of Comparative Biomedical Sciences, Teramo, Italy.
authors: Piermarino
authors: P
authors: Milillo
authors: Donato
authors: D
authors: Traversa
authors: Gabriella
authors: G
authors: Elia
authors: Domenico
authors: D
authors: Otranto
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D004175:Diptera / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006734:Horse Diseases / Q000469:parasitology; D006736:Horses; D007814:Larva / Q000276:immunology; D009198:Myiasis / Q000469:parasitology / Q000662:veterinary; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000276:immunology
chemical_list: D000941:Antigens; D012471:Salivary Proteins and Peptides