Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor.
A low-cost microBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a microBR, a conventional bench scale bioreactor and a shake flask. Under the microBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 microg/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the microBR were comparable to those obtained using conventional scale methods.
Rahman
Pattanathu K S M
PK
Chemical and Bioprocess Engineering Group, School of Science and Engineering, Teesside University, Middlesbrough TS1 3BA, Tees Valley, UK. p.rahman@tees.ac.uk
Pasirayi
Godfrey
G
Auger
Vincent
V
Ali
Zulfiqur
Z
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D019149:Bioreactors; D004867:Equipment Design; D006017:Glycolipids / Q000378:metabolism*; D044085:Microfluidics / Q000295:instrumentation*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011710:Pyocyanine / Q000378:metabolism* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; D011710:Pyocyanine