Evaluation of gallbladder lipid level during carcinogenesis by an infrared spectroscopic method.
BACKGROUND
Fourier transform infrared (FTIR) spectroscopy is sensitive to the molecular composition of tissue and has the potential to identify premalignant tissue. Our previous studies found that the lipids band of FTIR decreased in malignant tissues compared to normal tissue but increased in the cell line.


OBJECTIVE
To investigate the change of lipids during carcinogenesis in the gallbladder by FTIR spectroscopy.


METHODS
The tissue from 12 malignant samples and 10 normal samples together with their corresponding tissue plasma membrane and gallbladder cancer cell lines were observed by FTIR.


RESULTS
Specific changes of lipids were observed in the FTIR spectral features of tissue, cell, and plasma membrane. The CH3 stretching band at 2,957 cm(-1) and the CH2 stretching bands at 2,853 cm(-1) decreased in the malignant tissue but increased in the tissue plasma membrane; the C-O stretching band at 1,740 cm(-1) disappeared in the malignant tissue but significantly increased in the tissue plasma membrane. The intensity of these bands all increased in the cancer cell line. The ratio of intensity (I) of 1,460 cm(-1)/1,398 cm(-1) was smaller in malignant tissue and the tissue plasma membrane.


CONCLUSIONS
Lipids were increased in the plasma membrane during carcinogenesis of the gallbladder; the ratio of intensity (I) 1,460 cm(-1)/1,398 cm(-1) could be a marker to diagnose cancer by FTIR.
Wang
Jiansheng
J
Department of Surgical Oncology, First Hospital of Xi'an Jiaotong University, 710061, Xi'an, China. wangjsh@mail.xjtu.edu.cn
Zhang
Jia
J
Wu
Wenan
W
Duan
Xiaoyi
X
Wang
Sicen
S
Zhang
Mingxin
M
Zhou
Suna
S
Mo
Fei
F
Xu
Yizhuang
Y
Shi
Jingsen
J
Wu
Jinguang
J
D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005706:Gallbladder Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism*; D008875:Middle Aged; D011237:Predictive Value of Tests; D017550:Spectroscopy, Fourier Transform Infrared; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D008563:Membrane Lipids