Discovery of short pseudogenes derived from messenger RNAs.
More than 40% of the human genome is generated by retrotransposition, a series of in vivo processes involving reverse transcription of RNA molecules and integration of the transcripts into the genomic sequence. The mechanism of retrotransposition, however, is not fully understood, and additional genomic elements generated by retrotransposition may remain to be discovered. Here, we report that the human genome contains many previously unidentified short pseudogenes generated by retrotransposition of mRNAs. Genomic elements generated by non-long terminal repeat retrotransposition have specific sequence signatures: a poly-A tract that is immediately downstream and a pair of duplicated sequences, called target site duplications (TSDs), at either end. Using a new computer program, TSDscan, that can accurately detect pseudogenes based on the presence of the poly-A tract and TSDs, we found 654 short (< or = 300 bp), previously unknown pseudogenes derived from mRNAs. Comprehensive analyses of the pseudogenes that we identified and their parent mRNAs revealed that the pseudogene length depends on the parent mRNA length: long mRNAs generate more short pseudogenes than do short mRNAs. To explain this phenomenon, we hypothesize that most long mRNAs are truncated before they are reverse transcribed. Truncated mRNAs would be rapidly degraded during reverse transcription, resulting in the generation of short pseudogenes.
Terai
Goro
G
INTEC Systems Institute Inc., Koto-ku 136-0075, Japan. terai_goro@intec-si.co.jp
Yoshizawa
Aya
A
Okida
Hiroaki
H
Asai
Kiyoshi
K
Mituyama
Toutai
T
D000465:Algorithms; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D011061:Poly A / Q000032:analysis; D011544:Pseudogenes; D012333:RNA, Messenger / Q000737:chemistry*; D018626:Retroelements
D012333:RNA, Messenger; D018626:Retroelements; D011061:Poly A