v-Raf murine sarcoma viral oncogene mutation status in serous borderline ovarian tumors and the effect on clinical behavior.
OBJECTIVE
To determine the incidence of activating v-raf murine sarcoma viral oncogene (BRAF) mutations in 30 serous borderline tumors (SBTs) of the ovary and the accompanying implants and to link BRAF mutation status to the clinical behavior of these tumors.


RESULTS
Serous borderline tumors and noninvasive implants of 30 patients were analyzed for the presence of the BRAF V599E mutation, and mutation status was correlated to 70 months of clinical follow-up. Mutation status could be assessed in 27 SBTs. Eleven (41%) showed a BRAF mulation. Four (80%) of 5 patients with bilateral SBT showed a BRAF mutation in both ovaries. From the 8 implants that were analyzed for BRAF, 2 (25%) were mutated together with their primary tumor. v-Raf murine sarcoma viral oncogene mutation positive SBTs tend to present with a lower International Federation of Gynecology and Obstetrics stage and a higher tumor volume and are less frequently aneuploid. Seventy months' follow-up indicated no significant recurrence-free survival difference between these groups.


CONCLUSIONS
v-Raf murine sarcoma viral oncogene mutations are common in ovarian SBT, are strongly associated with bilateral tumors, and are also found in implants. A larger number of tumors should be investigated to assess clinical importance of BRAF mutation status in SBTs.
Verbruggen
Marjolijn B
MB
Department of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, the Netherlands.
Sieben
Nathalie L G
NL
Roemen
Guido M J M
GM
Rockx
Davy A P
DA
van Diest
Paul J
PJ
Verheijen
René H M
RH
Dorsman
Josephine C
JC
D000328:Adult; D018284:Cystadenocarcinoma, Serous / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D019073:HT29 Cells; D006801:Humans; D008875:Middle Aged; D009154:Mutation / Q000502:physiology; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012189:Retrospective Studies
C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf