title: A critical evaluation of influence of ethanol and diet on salsolinol enantiomers in humans and rats.
abstract: BACKGROUND
  (R/S)-Salsolinol (SAL), a condensation product of dopamine (DA) with acetaldehyde, has been speculated to have a role in the etiology of alcoholism. Earlier studies have shown the presence of SAL in biological fluids and postmortem brains from both alcoholics and nonalcoholics. However, the involvement of SAL in alcoholism has been controversial over several decades, since the reported SAL levels and their changes after ethanol exposure were not consistent, possibly due to inadequate analytical procedures and confounding factors such as diet and genetic predisposition. Using a newly developed mass spectrometric method to analyze SAL stereoisomers, we evaluated the contribution of ethanol, diet, and genetic background to SAL levels as well as its enantiomeric distribution.
  
  
  METHODS
  Simultaneous measurement of SAL enantiomers and DA were achieved by high performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). Plasma samples were collected from human subjects before and after banana (a food rich in SAL) intake, and during ethanol infusion. Rat plasma and brain samples were collected at various time points after the administration of SAL or banana by gavage. The brain parts including nucleus accumbens (NAC) and striatum (STR) were obtained from alcohol-non-preferring (NP) or alcohol-preferring (P) rats as well as P-rats which had a free access to ethanol (P-EtOH).
  
  
  RESULTS
  Plasma SAL levels were increased significantly after banana intake in humans. Consistently, administration of banana to rats also resulted in a drastic increase of plasma SAL levels, whereas brain SAL levels remained unaltered. Acute ethanol infusion did not change SAL levels or R/S ratio in plasma from healthy humans. The levels of both SAL isomers and DA were significantly lower in the NAC of P rats in comparison to NP rats. The SAL levels in NAC of P rats remained unchanged after chronic free-choice ethanol drinking. There were decreasing trends of SAL in STR and DA in both brain regions. No changes in enantiomeric ratio were observed after acute or chronic ethanol exposure.
  
  
  CONCLUSIONS
  SAL from dietary sources is the major contributor to plasma SAL levels. No significant changes of SAL plasma levels or enantiomeric distribution after acute or chronic ethanol exposure suggest that SAL may not be a biomarker for ethanol drinking. Significantly lower SAL and DA levels observed in NAC of P rats may be associated with innate alcohol preference.
authors: Laboratory of Molecular Signaling, National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, USA.
authors: Jeongrim
authors: J
authors: Lee
authors: Vijay A
authors: VA
authors: Ramchandani
authors: Kei
authors: K
authors: Hamazaki
authors: Eric A
authors: EA
authors: Engleman
authors: William J
authors: WJ
authors: McBride
authors: Ting-Kai
authors: TK
authors: Li
authors: Hee-Yong
authors: HY
authors: Kim
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000235:genetics / Q000523:psychology; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D004298:Dopamine / Q000097:blood; D000431:Ethanol / Q000494:pharmacology*; D005504:Food Analysis; D006801:Humans; D007546:Isoquinolines / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D028521:Musa; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D001679:Biogenic Amines; D002492:Central Nervous System Depressants; D007546:Isoquinolines; D000431:Ethanol; C036617:salsolinol; D004298:Dopamine