Dexamethasone effects on Bax expression in the mouse testicular germ cells.
Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. The aim of the present study was to find out whether dexamethasone (Dex), a widely used GC, would influence the apoptosis and expression of Bax, an important proapoptotic protein, in the mouse testicular germ cells. Experimental groups of 8 male NMRI mice received one of the following treatments daily for 7 days: 4, 7 and 10 mg/kg Dex. Control groups were treated with equivalent volumes of saline. Experimental and control animals were sacrificed 24 h after the last injection. Immunohistochemical procedure was used to evaluation of Bax expression and the deoxyuridine nick-end labeling (TUNEL) was applied to assessment of the apoptotic germ cells. Bax expression was upregulated mainly at stages VII-VIII of spermatogenic cycle (p<0.05) in experimental groups. Apoptotic index was significantly increased in 7 and 10 mg/kg Dex treated mice (p<0.05). It appears that GCs such as Dex could induce apoptosis through the expression of proapoptotic proteins.
Mahmoud
Hashemitabar
H
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundi-Shapour University of Medical Sciences, Ahwaz - Iran.
Mahmoud
Orazizadeh
O
Layasadat
Khorsandi
K
Naeim
Albughobeish
A
D000818:Animals; D017209:Apoptosis; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D051028:bcl-2-Associated X Protein; D003907:Dexamethasone