Reversible enzymatic synthesis of flavin-adenine dinucleotide.
An enzyme has been partially purified from brewer's yeast which catalyzes the reversible reaction between riboflavin phosphate and adenosine triphosphate (ATP) on the one hand and flavin-adenine dinucleotide (FAD) and inorganic pyrophosphate on the other in the presence of magnesium ions. ATP could not be replaced by adenosine diphosphate or adenosine-5-phosphate, and in the reverse reaction, inorganic pyrophosphate could not be replaced by ortho- or metaphosphate. With riboflavin and ATP a slight amount of FAD formation was observed.
SCHRECKER
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KORNBERG
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D000244:Adenosine Diphosphate; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate / Q000378:metabolism*; D004795:Enzyme Stability; D005486:Flavin Mononucleotide; D005182:Flavin-Adenine Dinucleotide / Q000096:biosynthesis* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D043564:Inorganic Pyrophosphatase / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry*; D011755:Pyrophosphatases / Q000378:metabolism*; D012256:Riboflavin / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins; D005182:Flavin-Adenine Dinucleotide; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D005486:Flavin Mononucleotide; D000255:Adenosine Triphosphate; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase; C021899:nucleotide pyrophosphatase; D008274:Magnesium; D012256:Riboflavin