Effects of ovariectomy in prepubertal goats.
The objectives of this study were to determine the effects of ovariectomy on mammary gland development in prepubertal goats and to validate this model to study mammogenesis in young dairy ruminants. In this experiment, 3 months of aged goats were ovariectomized (ovx) while shammed goats played as surgery controls (sham). Thereafter, sham and ovx goats were slaughtered at 7 months of age to provide tissue for the assays. Results demonstrated that proliferation of mammary of mammary epithelial cells was significantly lower in ovariectomized goats compared to control goats. In ovx animal, epithelium structures were completely overstretched and epithelial ducts were undeveloped with limited branching whereas control animals had classical complex arborescent units with multiple round ductules and limited stroma. Concerning ERalpha (estrogen receptor alpha), PR (progesterone receptor) and P450 (aromatase) expression, results showed number of ERalpha, PR and P450 positive cells was higher in shammed goats compared to ovariectomized goats. All this results suggested that goat mammogenesis and ovarian control are similar to prepubertal heifers and that young goats are a good model to study mammary gland development in ruminants. In conclusion, we demonstrated that ovariectomy of prepubertal goats decreased proliferation of mammary epithelial cells with a profound alteration of cell adhesion molecules.
Dessauge
F
F
INRA, Agrocampus Ouest, UMR1080 Dairy Production, Saint Gilles, France. Frederic.Dessauge@rennes.inra.fr
Finot
L
L
Wiart
S
S
Aubry
J M
JM
Ellis
S E
SE
D000818:Animals; D001141:Aromatase / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D006041:Goats / Q000254:growth & development* / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D011980:Receptors, Progesterone / Q000378:metabolism; D012741:Sexual Maturation / Q000502:physiology*
D015815:Cell Adhesion Molecules; D047628:Estrogen Receptor alpha; D011980:Receptors, Progesterone; D001141:Aromatase