Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity.
Immune cells navigate through different environments where they experience different mechanical forces. Responses to external forces are determined by the mechanical properties of a cell and they depend to a large extent on the actin-rich cell cortex. We report here that Myo1G, a previously uncharacterised member of class I myosins, is expressed specifically in haematopoietic tissues and cells. It is associated with the plasma membrane. This association is dependent on a conserved PH-domain-like myosin I tail homology motif and the head domain. However, the head domain does not need to be a functional motor. Knockdown of Myo1G in Jurkat cells decreased cell elasticity significantly. We propose that Myo1G regulates cell elasticity by deformations of the actin network at the cell cortex.
Olety
Balaji
B
Institute of General Zoology and Genetics, Westfalian Wilhelms-University Münster, Münster, Germany.
Wälte
Mike
M
Honnert
Ulrike
U
Schillers
Hermann
H
Bähler
Martin
M
D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D004548:Elasticity / Q000502:physiology*; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology*; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009218:Myosins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034741:RNA, Small Interfering; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D000199:Actins; D034741:RNA, Small Interfering; D009218:Myosins