title: IL-5 expression and release from human CD34 cells in vitro; ex vivo evidence from cases of asthma and Churg-Strauss syndrome.
abstract: BACKGROUND
  Eosinophils develop from hematopoietic CD34(+) progenitor cells in the bone marrow (BM) under the influence of Interleukin-5 (IL-5). The primary source of IL-5 is T-lymphocytes, although other sources may exist. The aims of this study were to determine whether CD34(+) cells from human peripheral blood (PB) and BM have the capacity to produce IL-5 when stimulated in vitro, and secondly, whether an elevated number of IL-5-producing CD34(+) cells can be found in situ in ongoing eosinophilic disease.
  
  
  METHODS
  CD34(+) cells from PB and BM were stimulated in vitro, and IL-5 production and release was assessed by ELISA, ELISPOT, flow cytometry and immunocytochemistry. Blood and BM from a patient with Churg-Strauss syndrome were analyzed by flow cytometry for CD34(+)/IL-5(+) cells, and immunohistochemical staining of CD34(+)/IL-5(+) cells in bronchial biopsies from an asthmatic patient was performed.
  
  
  RESULTS
  Both PB and BM CD34(+) cells can produce and release IL-5 when stimulated in vitro. In the Churg-Strauss patient, IL-5-producing CD34(+) cells were found in PB and BM. Oral glucocorticoid treatment markedly decreased the number of IL-5-positive CD34 cells in the BM. CD34(+)/IL-5(+) cells were present in a patient with asthma.
  
  
  CONCLUSIONS
  CD34(+) cells in blood and BM are capable of producing IL-5 both in vitro and in vivo in humans, arguing that these cells may have the capacity to contribute to eosinophilic inflammation. Consequently, targeting CD34(+) progenitor cells that produce and release IL-5 may be effective in reducing the mobilization of eosinophil lineage-committed cells in eosinophilic-driven diseases.
authors.affiliation: Krefting Research Centre, Department of Internal Medicine, Institute of Medicine, Göteborg University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Bossios
authors.forename: M
authors.initials: M
authors.lastname: Sjöstrand
authors.forename: A-K
authors.initials: AK
authors.lastname: Dahlborn
authors.forename: K
authors.initials: K
authors.lastname: Samitas
authors.forename: C
authors.initials: C
authors.lastname: Malmhäll
authors.forename: M
authors.initials: M
authors.lastname: Gaga
authors.forename: J
authors.initials: J
authors.lastname: Lötvall
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology / Q000378:metabolism*; D001249:Asthma / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation; D015267:Churg-Strauss Syndrome / Q000276:immunology / Q000378:metabolism*; D004802:Eosinophilia / Q000209:etiology / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D015848:Interleukin-5 / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D018952:Antigens, CD34; D015848:Interleukin-5