Towards a genetic map for Anopheles albimanus: identification of microsatellite markers and a preliminary linkage map for chromosome 2.
Fifty microsatellite loci were identified in the malaria vector Anopheles albimanus. Markers segregating in F2 progeny of crosses between laboratory strains of An. albimanus were used to construct a preliminary genetic map. More than 300 progeny were genotyped, but the resolution of the map was limited by the lack of polymorphisms in the microsatellite alleles. A robust linkage map for chromosome 2 was established, and additional markers were assigned to the third and X chromosomes by linkage to morphological markers of known physical location. Additional non-informative microsatellite sequences are provided including some showing similarity to those of An. gambiae. This study significantly increases the number of genetic markers available for An. albimanus and provides useful tools for population genetics and genetic mapping studies in this important malaria vector.
Penilla
R Patricia
RP
Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Tapachula, Chiapas, México. penilla@insp.mx
Ranson
Hilary
H
Padilla
Norma
N
Morgan
John C
JC
Steen
Keith
K
Pignatelli
Patricia
P
Rodríguez
Américo D
AD
Hemingway
Janet
J
Brogdon
William G
WG
Black
William C
WC
Benedict
Mark Q
MQ
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*; D014960:X Chromosome