Mycoplasma synoviae cell invasion: elucidation of the Mycoplasma pathogenesis in chicken.
Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was detected initially adhered to and subsequently inside the host cells. Between 24 and 48 h of infection, Ms was detected in the perinuclear region, and after 72 h of infection was confirmed by gentamicin invasion assay. High and low passage Ms strains showed no differences in adherence or invasion. The morphology and the actin filaments of the infected HEp-2 cells were preserved throughout the study period. The observed invasion by Ms is consistent with the biology of Mollicutes, and could explain the difficulties in recovering field isolates of the mycoplasma and in controlling the infection in birds even after long-term antibiotic treatment.
Buim
Marcos R
MR
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
Buzinhani
Melissa
M
Yamaguti
Maurício
M
Oliveira
Rosângela C
RC
Mettifogo
Elena
E
Ueno
Priscila M
PM
Timenetsky
Jorge
J
Santelli
Gláucia M M
GM
Ferreira
Antonio J Piantino
AJ
D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D002645:Chickens; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D005456:Fluorescent Dyes; D006801:Humans; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000502:physiology*; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology*; D013194:Staining and Labeling
D005456:Fluorescent Dyes