Intense correlation between protein-conjugated acrolein and primary Sjögren's syndrome.
BACKGROUND
We recently found that an increased plasma concentration of protein-conjugated acrolein is a good biomarker for stroke. Therefore we determine whether the concentration of protein-conjugated acrolein is increased in saliva from patients with primary Sjögren's syndrome.


METHODS
Stimulated whole-mixed saliva was collected from 10 patients and 13 control subjects. The concentration of protein-conjugated acrolein in saliva and plasma was measured by either Western blotting or enzyme-linked immunosorbent assay.


RESULTS
The concentration of protein-conjugated acrolein, especially albumin-conjugated acrolein, was greatly increased in saliva from patients with primary Sjögren's syndrome (p<0.001). The concentration of protein-conjugated acrolein was inversely correlated with the flow rate of saliva.


CONCLUSIONS
The results indicate that the concentration of protein-conjugated acrolein, a marker of cell or tissue damage, in saliva is well correlated with seriousness of primary Sjögren's syndrome.
Higashi
Kyohei
K
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Yoshida
Madoka
M
Igarashi
Atsuko
A
Ito
Kayoko
K
Wada
Yoko
Y
Murakami
Shuichi
S
Kobayashi
Daisuke
D
Nakano
Masaaki
M
Sohda
Miwa
M
Nakajima
Takako
T
Narita
Ichiei
I
Toida
Toshihiko
T
Kashiwagi
Keiko
K
Igarashi
Kazuei
K
D000171:Acrolein / Q000097:blood* / Q000737:chemistry; D000368:Aged; D000418:Albumins / Q000737:chemistry*; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008239:Lysine / Q000097:blood* / Q000737:chemistry; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000097:blood*; D000516:alpha-Amylases / Q000097:blood* / Q000737:chemistry / Q000378:metabolism
D000418:Albumins; D015415:Biomarkers; D000171:Acrolein; D000516:alpha-Amylases; D008239:Lysine