Polymerase chain reaction based scaffold preparation for the production of thin, branched DNA origami nanostructures of arbitrary sizes.
Designs for DNA origami have previously been limited by the size of the available single-stranded genomes for scaffolds. Here we present a straightforward method for the production of scaffold strands having various lengths, using polymerase chain reaction amplification followed by strand separation via streptavidin-coated magnetic beads. We have applied this approach in assembling several distinct DNA nanostructures that have thin ( approximately 10 nm) features and branching points, making them potentially useful templates for nanowires in complex electronic circuitry.
Pound
Elisabeth
E
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, USA.
Ashton
Jeffrey R
JR
Becerril
Héctor A
HA
Woolley
Adam T
AT
D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods*; D013499:Surface Properties
D046911:Macromolecular Substances; D004247:DNA