title: Antioxidant principles of Tanacetum vulgare L. aerial parts.
abstract: The methanolic extract of aerial parts of Tanacetum vulgare L. (Asteraceae) and its fractions were investigated for antioxidant activity. The crude extract displayed DPPH radical scavenging effects with an EC50 value of 37 +/- 1.2 microg/mL (n=3). Activity-guided fractionations of the crude extract resulted in the isolation of three antioxidant compounds; 3,5-O-dicaffeoylquinic acid (3,5-DCQA), axillarin and luteolin. 3,5-DCQA was the major constituent with antioxidant activity (IC50 = 9.7 microM) comparable with that of the standard quercetin (IC50 = 8.8 microM). Though the isolated compounds were previously known for their antioxidant effects, this is the first report on the identification of 3,5-DCQA from Tanacetum vulgare. The displayed potent antioxidant activity of the crude extract and isolated active principles is in support of the traditional medicinal uses of the plant for disease conditions such as wound healing, rheumatic arthritis and other inflammatory conditions.
authors.affiliation: Pharmacognosy Research Laboratories, Medway School of Science, The University of Greenwich, Central Avenue, Chatham-Maritime, Kent, ME4 4TB, UK.
authors.forename: Malindra
authors.initials: M
authors.lastname: Juan-Badaturuge
authors.forename: Solomon
authors.initials: S
authors.lastname: Habtemariam
authors.forename: Caroline
authors.initials: C
authors.lastname: Jackson
authors.forename: Michael J K
authors.initials: MJ
authors.lastname: Thomas
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D016877:Oxidants / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D031203:Tanacetum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl