title: Synthesis of gold microplates using bovine serum albumin as a reductant and a stabilizer.
abstract: Gold microplates were synthesized in aqueous solutions by reducing HAuCl(4) with the hydroxyl groups in both serine and threonine of bovine serum albumin (BSA), which is a globular protein in its native state. In this article, we systematically investigated the effects of temperature, pH value, the concentration of BSA, and ionic species on the reduction kinetics and thus the size and morphology of the final product. The optimal experimental conditions for producing uniform Au microplates include the following: an elevated temperature in the range of 55-65 degrees C, an acidic solution with pH approximately 3, and the presence of NaCl (0.14 M). We found that if any one of these parameters was deviated from the optimal condition, Au microplates would not be formed in high yields. We also found that the surfaces of the as-synthesized Au microplates were covered by a dense array of BSA bumps.
authors.affiliation: Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, USA.
authors.forename: Leslie
authors.initials: L
authors.lastname: Au
authors.forename: Byungkwon
authors.initials: B
authors.lastname: Lim
authors.forename: Peter
authors.initials: P
authors.lastname: Colletti
authors.forename: Young-Shin
authors.initials: YS
authors.lastname: Jun
authors.forename: Younan
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D002417:Cattle; D005079:Excipients / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D019163:Reducing Agents / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013696:Temperature
chemical_list: D005079:Excipients; D019163:Reducing Agents; D012710:Serum Albumin, Bovine; D006046:Gold