[Comparison of modified Bethesda assay and Nijmegen assay in detecting FVII inhibitor in patients with hemophilia A].
OBJECTIVE
To compare the sensitivity and practicability of modified Bethesda assay and Nijmegen assay in detecting factor VIII (FVIII) inhibitor.


METHODS
Modified Bethesda assay and Nijmegen assay were used to screen FVIII inhibitors in 237 patients with hemophilia A. The buffer plus universal coagulation reference plasma (UCRP) was used to establish a standard curve for FVIII: C assay in modified Bethesda method, instead of Nijmegen plasma plus FVIII deficiency plasma in Nijmegen method. The cutoff value for positive FVIII inhibitors is > or = 0.6 BU/ml.


RESULTS
The positive rate of FVIII inhibitors was 5.5% (n = 13) when using modified Bethesda assay and was 8.4% (n = 20) when using Nijmegen assay (P > 0.05).


CONCLUSIONS
Modified standard Bethesda assay is a convenient and feasible method for detecting FVIII inhibitors.
Fan
Lian-Kai
LK
Department of Hematology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Wang
Zhi-Wei
ZW
Hua
Bao-Lai
BL
Su
Wei
W
Wang
Shu-Jie
SJ
Zhao
Yong-Qiang
YQ
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001780:Blood Coagulation Tests / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
D001323:Autoantibodies; D005169:Factor VIII