title: BMP Inhibition with dorsomorphin limits adipogenic potential of preadipocytes.
abstract: Previous studies revealed that bone morphogenetic protein (BMP) induces commitment to the adipocyte lineage in pluripotent stem cells. The present study explored the role of endogenous BMP activity in 3T3-L1 preadipocytes. The expression of phospho-Smad1/5/8 was monitored because BMP transmits its signal through Smad1/5/8 phosphorylation. Phosphorylated Smad1/5/8 was higher in proliferating preadipocytes, and lower in differentiating adipocytes after removing differentiation inducers. Reporter assays revealed that dorsomorphin predominantly inhibits the BMP pathway but not the structurally related TGF-beta/activin pathway. The addition of dorsomorphin to the culture medium prior to treatment with differentiation inducers impaired lipid accumulation in 3T3-L1 cells. The present study indicated that activation of BMP signaling in preadipocytes is required for these cells to initiate the adipogenic program.
authors.affiliation: Division of Applied Biosciences, Kyoto University Graduate School of Agriculture, Kitashirakawa Oiwakecho, Kyoto 606-8502, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Suenaga
authors.forename: Tohru
authors.initials: T
authors.lastname: Matsui
authors.forename: Masayuki
authors.initials: M
authors.lastname: Funaba
mesh_terms: D041721:3T3-L1 Cells / Q000166:cytology / Q000187:drug effects; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D051379:Mice; D011720:Pyrazoles / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D019485:Bone Morphogenetic Proteins; D011720:Pyrazoles; D011743:Pyrimidines; C516138:dorsomorphin