Synergistic targeting of alphavbeta3 integrin and galectin-1 with heteromultivalent paramagnetic liposomes for combined MR imaging and treatment of angiogenesis.
Effective and specific targeting of nanoparticles is of paramount importance in the fields of targeted therapeutics and diagnostics. In the current study, we investigated the targeting efficacy of nanoparticles that were functionalized with two angiogenesis-specific targeting ligands, an alpha(v)beta(3) integrin-specific and a galectin-1-specific peptide. We show in vitro, using optical techniques and MRI, that the dual-targeting approach produces synergistic targeting effects, causing a dramatically elevated uptake of nanoparticles as compared to single ligand targeting.
Kluza
Ewelina
E
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands. E.Kluza@tue.nl
van der Schaft
Daisy W J
DW
Hautvast
Petra A I
PA
Mulder
Willem J M
WJ
Mayo
Kevin H
KH
Griffioen
Arjan W
AW
Strijkers
Gustav J
GJ
Nicolay
Klaas
K
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D002478:Cells, Cultured; D037483:Galectin 1 / Q000737:chemistry*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000737:chemistry*; D008024:Ligands; D008081:Liposomes; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009389:Neovascularization, Pathologic; D010455:Peptides / Q000737:chemistry
D020533:Angiogenesis Inhibitors; D037483:Galectin 1; D039302:Integrin alphaVbeta3; D008024:Ligands; D008081:Liposomes; D010455:Peptides