title: Influence of growth phase and geographic origin on the essential oil composition of Pituranthos chloranthus from Tunisia.
abstract: The chemical compositions of the essential oils of Pituranthos chloranthus harvested at the vegetative, flower budding, flowering and fruiting stages from three distinct geographical areas of Tunisia were investigated using GC-FID and GC-MS. One hundred and fifty compounds were identified in which alpha-pinene, beta-pinene, alpha-phellandrene, beta-myrcene, beta-phellandrene, p-cymene, 8-methyldecanal, exo-2-hydroxycineole acetate and carvacrol could reach more than 10% of the total amount. However, this composition varied with respect to both the geographical area and the season. A clear discrimination of samples could be achieved by submitting the results to PLS discriminant analysis. p-Cymenene was only detected at the floral budding stage (February), whereas high amounts of exo-2-hydroxycineole and exo-2-hydroxycineole acetate were specific for the flowering period (April). Carvacrol was showed to be characteristic mainly of the fruiting period (August), whereas the vegetative state (November) could be distinguished from the others by the presence of alpha- and beta-pinene. Limonene, camphene, geraniol and beta-damascenone were likely to be specific for the essential oils of this species collected from the different regions of Tunisia.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Facultd de Pharmacie de Monastir, Rue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Aïcha
authors.initials: A
authors.lastname: Neffati
authors.forename: Didier
authors.initials: D
authors.lastname: Hennequin
authors.forename: Benoît
authors.initials: B
authors.lastname: Basset
authors.forename: Leïla
authors.initials: L
authors.lastname: Chekir-Ghedira
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Daniel
authors.initials: D
authors.lastname: Barillier
authors.forename: Jérôme
authors.initials: J
authors.lastname: Ledauphin
mesh_terms: D002855:Chromatography, Thin Layer; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development*; D012621:Seasons; D012717:Sesquiterpenes / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013729:Terpenes / Q000737:chemistry; D014416:Tunisia
chemical_list: D009822:Oils, Volatile; D012717:Sesquiterpenes; D013729:Terpenes