title: Antimicrobial activity of the cell organelles, lysosomes isolated from egg white.
abstract: Lysosomes as a cell organelle type, are safe biological control agents that may be possible replacements for chemical antimicrobial agents because they are simply isolated from egg white. In this study, it was found that the lysosomes isolated from egg white exhibited pH-dependent antimicrobial activity with the optimal activity found at pH 6.0. The efficiency of lysosomes in inhibiting bacterial growth and activity was evaluated over a 12-hour treatment period. Seven different microorganisms were used as bacterial strains, and the lysosomes showed a significant antimicrobial effect against all strains. In addition, the antimicrobial activity was maintained for 100 days, and there did not appear to be any resistance of E. coli to the lysosomal activity up to eighth culture. However, the lysosomes did not affect the viability of mammalian cells, suggesting the biocompatibility of lysosomes. These highly effective lysosomes have a bright future in the application of novel antimicrobial sources as a cell organelle type.
authors.affiliation: Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Jihee
authors.initials: J
authors.lastname: Yoon
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Ki-Ju
authors.initials: KJ
authors.lastname: Kim
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D024881:Drug Resistance, Bacterial; D004529:Egg White; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents