Probing the dynamic differential stiffness of dsDNA interacting with RecA in the enthalpic regime.
RecA plays a central role in homologous recombination of DNA. When RecA combines with dsDNA to form RecA-dsDNA nucleofilament, it unwinds dsDNA and changes its structure. The unwinding length extension of a DNA segment interacting with RecA has been studied by various techniques, but the dynamic differential stiffness of dsDNA conjugating with RecA has not been well characterized. We applied oscillatory optical tweezers to measure the differential stiffness of dsDNA molecules, interacting with RecA, as a function of time at a constant stretching force of 33.6pN. The values of the differential stiffness of DNA (for stretching force in the range of 20.0pN to 33.6pN) measured by oscillatory optical tweezers, both before and after its interaction with RecA, are consistent with those measured by stationary optical tweezers. In the dynamic measurement, we have shown that the association (or binding) rate increases with higher concentration of RecA; besides, we have also monitored in real-time the dissociation of RecA from the stretched RecA-dsDNA filament as ATPgammaS was washed off from the sample chamber. Finally, we verified that RecA (I26C), a form of RecA mutant, does not affect the differential stiffness of the stretched DNA sample. It implies that mutant RecA (I26C) does not bind to the DNA, which is consistent with the result obtained by conventional biochemical approach.
Lien
Chia-Hui
CH
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Wei
Ming-Tzo
MT
Tseng
Te-Yu
TY
Lee
Chien-Der
CD
Wang
Chung
C
Wang
Ting-Fang
TF
Ou-Yang
H Daniel
HD
Chiou
Arthur
A
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D055119:Elastic Modulus; D052898:Optical Tweezers; D009991:Oscillometry / Q000379:methods*; D011485:Protein Binding; D011938:Rec A Recombinases / Q000737:chemistry* / Q000648:ultrastructure*; D013314:Stress, Mechanical
D004247:DNA; D011938:Rec A Recombinases