title: Polymerase chain reaction based scaffold preparation for the production of thin, branched DNA origami nanostructures of arbitrary sizes.
abstract: Designs for DNA origami have previously been limited by the size of the available single-stranded genomes for scaffolds. Here we present a straightforward method for the production of scaffold strands having various lengths, using polymerase chain reaction amplification followed by strand separation via streptavidin-coated magnetic beads. We have applied this approach in assembling several distinct DNA nanostructures that have thin ( approximately 10 nm) features and branching points, making them potentially useful templates for nanowires in complex electronic circuitry.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, USA.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Pound
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Ashton
authors.forename: Héctor A
authors.initials: HA
authors.lastname: Becerril
authors.forename: Adam T
authors.initials: AT
authors.lastname: Woolley
mesh_terms: D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods*; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D004247:DNA