[Cometabolic transformation of high concentrations of 4-chlorophenol in an immobilized cell hollow fiber membrane bioreactor].
Pseudomonas putida grown suspendedly can cometabolize 4-chlorophenol (4-CP)in the presence of phenol (growth substrate). However, cometabolization process cannot be maintained due to the high toxicity of phenol and 4-CP to cells when the initial concentrations of phenol and 4-CP were 120 mg/L and 600 mg/L, respectively. Cells can degrade phenol and 4-CP at high concentrations after immobilization in hollow fiber membrane bioreactors. Even when the initial concentrations of phenol and 4-CP were 200 mg/L and 1000 mg/L, the substrates were completely biotransformed with in 34 hours. Different from free suspension cells, immobilized cells were protected by the hollow fiber membranes due to mass transfer limitation in the membranes. The cells can grow and degraded high concentration substrates.
Li
Yi
Y
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environmental Science and Engineering, Hohai University, Nanjing 210098, China. envly@hhu.edu.cn
Lei
Hong-ben
HB
D001431:Bacteriological Techniques / Q000379:methods; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D008567:Membranes, Artificial; D019800:Phenol / Q000378:metabolism; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*
D002733:Chlorophenols; D008567:Membranes, Artificial; D019800:Phenol; C029107:4-chlorophenol