title: Antihypertensive effect of peptide-enriched soy sauce-like seasoning and identification of its angiotensin I-converting enzyme inhibitory substances.
abstract: We have developed a peptide-enriched soy sauce-like seasoning termed Fermented Soybean Seasoning (FSS), by modifying the process of soy sauce brewing. The FSS has a 2.7-fold higher concentration of total peptides than regular soy sauce. The angiotensin I-converting enzyme (ACE) inhibitory activity of FSS (IC(50) = 454 microg/mL) was greater than that of regular soy sauce (IC(50) = 1620 microg/mL). The FSS demonstrated antihypertensive effects both in spontaneously hypertensive rats and in Dahl salt-sensitive rats during continuous feeding. The ACE inhibitory substances were purified from FSS by reversed-phase chromatography. Ala-Trp IC(50) = 10 microM; Gly-Trp IC(50) = 30 microM; Ala-Tyr IC(50) = 48 microM; Ser-Tyr, IC(50) = (67 microM; Gly-Tyr, IC(50) = 97 microM; Ala-Phe, IC(50) = 190 microM; Val-Pro, IC(50) = (480 microM; Ala-Ile, IC(50) = 690 microM; Val-Gly, IC(50) = 1100 microM; and a nicotianamine, IC(50) = 0.26 microM. [corrected] The concentrations of these substances in the FSS were revealed to be higher than that of regular soy sauce through quantitative LC-MS/MS analysis.
authors.affiliation: Research and Development Division, Kikkoman Corporation, 399 Noda, Noda, Chiba 278-0037, Japan.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nakahara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sano
authors.forename: Hitomi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Chikata
authors.forename: Emiko
authors.initials: E
authors.lastname: Kinoshita
authors.forename: Riichiro
authors.initials: R
authors.lastname: Uchida
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D001230:Aspergillus / Q000378:metabolism; D004195:Disease Models, Animal; D005285:Fermentation; D006801:Humans; D006973:Hypertension / Q000188:drug therapy*; D008297:Male; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D020303:Rats, Inbred Dahl; D011918:Rats, Inbred SHR; D045730:Soy Foods / Q000032:analysis* / Q000382:microbiology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides