Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines: comparison between two- and three-dimensional cell culture systems.
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.
Roncoroni
Leda
L
Department of Biology and Genetic for Health Sciences, University of Milan, Via Viotti, Milan, Italy. leda.roncoroni@unimi.it
Elli
Luca
L
Bardella
Maria Teresa
MT
Dogliotti
Elena
E
Grimoldi
Maria Grazia
MG
Viaggi
Silvia
S
Erba
Eugenio
E
Zunino
Annalisa
A
Doneda
Luisa
L
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000235:genetics*; D048430:Cell Shape; D002877:Chromosomes, Human; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization; D005434:Flow Cytometry; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D007621:Karyotyping; D018874:Spheroids, Cellular