title: Characterisation of the protective immune response following subcutaneous vaccination of susceptible mice against Trichuris muris.
abstract: Trichuris muris is a laboratory model for the human whipworm Trichuris trichiura which infects approximately 1 billion people in tropical and sub-tropical countries. The development of a vaccine would control trichuriasis by promoting the acquisition of immunity during childhood, thereby reducing faecal egg output by the community into their environment. Resistance to T. muris, defined as expulsion of the parasite prior to patency, requires the development of a T helper 2 (Th2) response during a primary infection. To our knowledge this is the first study to describe the protective immune response in the peripheral lymph nodes (PLN), mesenteric lymph nodes (MLN) and colonic mucosa following s.c. vaccination against T. muris. Susceptible AKR mice were either vaccinated with T. muris excretory-secretory product (ES) in incomplete Freund's adjuvant (IFA) (ES/IFA) or injected with PBS in IFA (PBS/IFA) and for protection experiments were infected with embryonated infective T. muris eggs 10 days later. The ES/IFA vaccine induced the proliferation of PLN cells and their production of Th2 cytokines and the Th1-associated cytokine IFN-gamma. Following a challenge infection, the ES/IFA vaccination offered susceptible mice complete protection. While MLN-derived IFN-gamma was produced by infected mice following either ES/IFA vaccination or PBS/IFA, the protection of susceptible mice by ES/IFA was characterised by the production of MLN-derived Th2 cytokines. Goblet cell hyperplasia and the influx and alternative activation of macrophages were observed locally in the gut post-challenge infection. The rate of epithelial turnover did not appear to be increased by vaccination, suggesting that there are differences in the mechanisms of expulsion between 'natural resistance' and 'vaccinated resistance'. High levels of serum IgG1 and cell-bound IgG1 in the colon of mice protected by the ES/IFA vaccine suggest that antibody may be involved in vaccination-induced worm expulsion.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK. H.Dixon-1@sms.ed.ac.uk
authors.forename: Helen
authors.initials: H
authors.lastname: Dixon
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Little
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Else
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005620:Freund's Adjuvant / Q000008:administration & dosage; D020397:Goblet Cells / Q000276:immunology; D018928:Immunity, Mucosal; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007279:Injections, Subcutaneous; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014257:Trichuriasis / Q000276:immunology / Q000517:prevention & control*; D014258:Trichuris / Q000276:immunology*; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D014612:Vaccines; D005620:Freund's Adjuvant