Seven consecutive successful clinical islet isolations with pancreatic ductal injection.
Inconsistent islet isolation is one of the issues of clinical islet transplantation. In the current study, we applied ductal injection to improve the consistency of islet isolation. Seven islet isolations were performed with the ductal injection of ET-Kyoto solution (DI group) and eight islet isolations were performed without the ductal injection (standard group) using brain-dead donor pancreata. Isolated islets were evaluated based on the Edmonton protocol for transplantation. The DI group had significantly higher islet yields (588,566 +/- 64,319 vs. 354,836 +/- 89,649 IE, p < 0.01) and viability (97.3 +/- 1.2% vs. 92.6 +/- 1.2%, p < 0.02) compared with the standard group. All seven isolated islet preparations in the DI group (100%), versus only three out of eight isolated islet preparations (38%) in the standard group met transplantation criteria. The islets from the DI group were transplanted into three type 1 diabetic patients and all three patients became insulin independent. Ductal injection significantly improved quantity and quality of isolated islets and resulted in high success rate of clinical islet transplantation. This simple modification will reduce the risk of failure of clinical islet isolation.
Matsumoto
Shinichi
S
Baylor Research Institute, Fort Worth, TX 76104, USA. shinichm@baylorhealth.edu
Noguichi
Hirofumi
H
Shimoda
Masayuki
M
Ikemoto
Tetsuya
T
Naziruddin
Bashoo
B
Jackson
Andrew
A
Tamura
Yoshiko
Y
Olson
Greg
G
Fujita
Yasutaka
Y
Chujo
Daisuke
D
Takita
Morihito
M
Kobayashi
Naoya
N
Onaca
Nicholas
N
Levy
Marlon
M
D000328:Adult; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery*; D004210:Dissection / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D007267:Injections / Q000379:methods; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods* / Q000592:standards; D008297:Male; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards; D016896:Treatment Outcome