"The heat is on": rapid microcalorimetric detection of mycobacteria in culture.
Detection of mycobacterial infection can be achieved by different means; however, culture-based methods remain the gold standard. In this paper, we present a new culture-based method using real-time microcalorimetric detection of growth of Mycobacterium species including Mycobacterium tuberculosis. Microcalorimetric detection of heat production by 6 different growing species of Mycobacterium was achieved between 20 and 310h depending on their type (fast vs. slow-growing mycobacteria) and initial concentration. This study demonstrates that microcalorimetric detection of mycobacterial growth is a potential advantageous alternative to methods using fluorescent or radiolabeled growth indicators.
Braissant
O
O
Lab. of Biomechanics and Biocalorimetry (LOB(2)), Faculty of Medicine, University of Basel, c/o Biozentrum - Pharmazentrum, Klingelbergstrasse 50-70, 4056 Basel, Switzerland. Olivier.braissant@unibas.ch <Olivier.braissant@unibas.ch>
Wirz
D
D
Göpfert
B
B
Daniels
A U
AU
D001431:Bacteriological Techniques / Q000379:methods*; D002151:Calorimetry / Q000379:methods; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000302:isolation & purification; D014376:Tuberculosis / Q000175:diagnosis*
D003470:Culture Media