title: Biological properties of a neutralized 2.5% sodium hypochlorite solution.
abstract: OBJECTIVE
  The objective of this study was to evaluate the influence of neutralizing a 2.5% NaOCl solution on its cytotoxicity, genotoxicity, and tissue-dissolving potential.
  
  
  METHODS
  The cytotoxicity and the genotoxicity of Dakin, a 2.5% NaOCl solution, and a neutralized 2.5% NaOCl solution were assessed according to ISO 10993 standards. The weight of palatal mucosa samples placed in neutralized 2.5% NaOCl, 2.5% NaOCl was recorded over time as well as the pH of the solutions.
  
  
  RESULTS
  The neutralized 2.5% NaOCl solution was 10-fold more cytotoxic than the 2.5% NaOCl solution. None of the solutions was genotoxic. The 2.5% NaOCl solution had a better tissue-dissolving capacity than the neutralized 2.5% NaOCl solution. The pH of the 2.5% NaOCl solution and neutralized 2.5% NaOCl solution decreased from 12 to 9 and from 7.5 to 5.6, respectively.
  
  
  CONCLUSIONS
  Neutralizing a 2.5% NaOCl solution increased its cytotoxicity, did not induce any genotoxic effect, and reduced its tissue-dissolving ability.
authors.affiliation: Laboratoire Interface Matrice Extracellulaire Biomatériaux, Faculté d'Odontologie, Marseille, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Aubut
authors.forename: Ludovic
authors.initials: L
authors.lastname: Pommel
authors.forename: Bernard
authors.initials: B
authors.lastname: Verhille
authors.forename: Thierry
authors.initials: T
authors.lastname: Orsière
authors.forename: Serge
authors.initials: S
authors.lastname: Garcia
authors.forename: Imad
authors.initials: I
authors.lastname: About
authors.forename: Jean
authors.initials: J
authors.lastname: Camps
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D004249:DNA Damage; D004338:Drug Combinations; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007739:L Cells; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000187:drug effects*; D012388:Root Canal Irrigants / Q000633:toxicity*; D017693:Sodium Bicarbonate / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity*; D034421:Sus scrofa
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D012388:Root Canal Irrigants; C069467:modified Dakin's solution; D017693:Sodium Bicarbonate; D012973:Sodium Hypochlorite