title: Three Dimensional OCT in the Engineering of Tissue Constructs: A Potentially Powerful Tool for Assessing Optimal Scaffold Structure.
abstract: Optical Coherence Tomography (OCT) provides detailed, real-time information on the structure and composition of constructs used in tissue engineering. The focus of this work is the OCT three-dimensional assessment of scaffolding architecture and distribution of cells on it. PLGA scaffolds were imaged in two and three-dimensions, both seeded and unseeded with cells. Then two types of scaffolds were reconstructed in three dimensions. Both scaffolding types were examined at three different seeding densities. The importance of three-dimensional assessments was evident, particularly with respect to porosity and identification of asymmetrical cell distribution.
authors.affiliation: Department of Orthopedic Surgery, Brigham & Women's Hospital, Boston, MA.
authors.forename: K
authors.initials: K
authors.lastname: Zheng
authors.forename: M A
authors.initials: MA
authors.lastname: Rupnick
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: M E
authors.initials: ME
authors.lastname: Brezinski