title: Towards a genetic map for Anopheles albimanus: identification of microsatellite markers and a preliminary linkage map for chromosome 2.
abstract: Fifty microsatellite loci were identified in the malaria vector Anopheles albimanus. Markers segregating in F2 progeny of crosses between laboratory strains of An. albimanus were used to construct a preliminary genetic map. More than 300 progeny were genotyped, but the resolution of the map was limited by the lack of polymorphisms in the microsatellite alleles. A robust linkage map for chromosome 2 was established, and additional markers were assigned to the third and X chromosomes by linkage to morphological markers of known physical location. Additional non-informative microsatellite sequences are provided including some showing similarity to those of An. gambiae. This study significantly increases the number of genetic markers available for An. albimanus and provides useful tools for population genetics and genetic mapping studies in this important malaria vector.
authors.affiliation: Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Tapachula, Chiapas, México. penilla@insp.mx
authors.forename: R Patricia
authors.initials: RP
authors.lastname: Penilla
authors.forename: Hilary
authors.initials: H
authors.lastname: Ranson
authors.forename: Norma
authors.initials: N
authors.lastname: Padilla
authors.forename: John C
authors.initials: JC
authors.lastname: Morgan
authors.forename: Keith
authors.initials: K
authors.lastname: Steen
authors.forename: Patricia
authors.initials: P
authors.lastname: Pignatelli
authors.forename: Américo D
authors.initials: AD
authors.lastname: Rodríguez
authors.forename: Janet
authors.initials: J
authors.lastname: Hemingway
authors.forename: William G
authors.initials: WG
authors.lastname: Brogdon
authors.forename: William C
authors.initials: WC
authors.lastname: Black
authors.forename: Mark Q
authors.initials: MQ
authors.lastname: Benedict
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*; D014960:X Chromosome