Oncogenic function of microtubule end-binding protein 1 in breast cancer.
Microtubule end-binding protein 1 (EB1) is an evolutionarily conserved protein that regulates microtubule dynamics and participates in diverse cell activities. Here, we demonstrate that EB1 expression is up-regulated in human breast cancer specimens and cell lines. The level of EB1 correlates with clinicopathological parameters indicating the malignancy of breast cancer, including higher histological grade, higher pathological tumour node metastasis (pTNM) stage, and higher incidence of lymph node metastasis. Knockdown of EB1 expression remarkably inhibits cancer cell proliferation, and conversely, elevation of its expression promotes cell proliferation. Our data further show that EB1 promotes colony formation and enhances tumour growth in nude mice. In addition, EB1 stimulates Aurora-B activity in breast cancer cells, and EB1 expression correlates with increased Aurora-B activity in clinical samples of breast cancer. These findings thus suggest an oncogenic role for EB1 in breast cancer.
Dong
Xin
X
Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Liu
Fangfang
F
Sun
Lei
L
Liu
Min
M
Li
Dengwen
D
Su
Dan
D
Zhu
Zhengmao
Z
Dong
Jin-Tang
JT
Fu
Li
L
Zhou
Jun
J
D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D009363:Neoplasm Proteins / Q000378:metabolism / Q000502:physiology*; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation
C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; C576921:AURKB protein, human; C576924:Aurkb protein, mouse; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases