Attachment of cultured fibroblasts and ultrastructural analysis of simulated cervical resorptions treated with high-power lasers and MTA.
OBJECTIVE
The present study evaluated the fibroblast attachment and the morphologic changes of simulated cervical root resorptions after irradiation with high-power lasers and the use of mineral trioxide aggregate (MTA).


METHODS
Standardized cavities were done on the coronal third of 40 single-rooted teeth and assigned into 4 groups according to the treatment applied: (1) untreated control, (2) use of MTA, (3) irradiation with Er:YAG laser (42 mJ, 10 Hz, 10 s), and (4) irradiation with high-power diode laser (1 W, 10 s). Fragments were prepared for SEM analysis: 6 samples of each group were plated with human gingival fibroblasts and 4 samples were used for ultrastructural analysis.


RESULTS
The cells' attachment was in decreasing order: G3 > G4 > G1> G2, with statistical differences among all groups (P < .05). The samples irradiated with Er:YAG exhibited roughness on the dentinal surface, no smear layer, and open dentinal tubules; those irradiated with diode laser exhibited a smooth surface, more smear layer, and closed dentinal tubules; and the control group showed high amount of smear layer and closed dentin tubules.


CONCLUSIONS
Irradiation with Er:YAG and diode lasers caused morphologic changes on the dentinal surfaces of simulated resorptions that favored cells' adhesion. MTA showed lower biocompatibility than irradiated groups but allowed cells' adhesion.
Raldi
Denise Pontes
DP
Division of Endodontics, Department of Dentistry, University of Taubate, Taubate, Sao Paulo, Brazil. pontesdenise@uol.com.br
Mello
Isabel
I
Neves
Ana Christina Claro
AC
Habitante
Sandra Marcia
SM
Miyagi
Suely Sueli Patrícia
SS
Lage-Marques
José Luiz
JL
D017607:Aluminum Compounds / Q000494:pharmacology / Q000627:therapeutic use; D000704:Analysis of Variance; D017610:Calcium Compounds / Q000494:pharmacology / Q000627:therapeutic use; D002448:Cell Adhesion; D002460:Cell Line; D003804:Dentin / Q000648:ultrastructure*; D003806:Dentin Permeability; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D010087:Oxides / Q000494:pharmacology / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000494:pharmacology / Q000627:therapeutic use*; D012391:Root Resorption / Q000473:pathology / Q000517:prevention & control*; D017640:Silicates / Q000494:pharmacology / Q000627:therapeutic use; D016369:Smear Layer; D013499:Surface Properties
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate