The alarm pheromone in male rats as a unique anxiety model: psychopharmacological evidence using anxiolytics.
Previously, we demonstrated that an alarm pheromone released from male donor Wistar rats evoked anxiety-related physiological and behavioral responses in recipient rats. Thus, we believe that this pheromone may increase anxiety levels in rats. In the current study, we evaluated the predictive validity of this alarm pheromone-induced anxiogenic effect in detail by investigating whether six types of human anxiolytics, each of which has a different mechanism of action, were efficacious in reducing anxiety, using changes in the acoustic startle reflex (ASR) as an index. The alarm pheromone-enhanced ASR was not affected by vehicle pretreatment but was dose-dependently attenuated by pretreatment with midazolam, phenelzine, propranolol, clonidine, and CP-154,526-although not buspirone. These results may reflect some aspects of the predictive validity of the alarm pheromone-induced anxiety in rats as an animal model of human anxiety.
Inagaki
Hideaki
H
Laboratory of Veterinary Ethology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. hhinaga@gipc.akita-u.ac.jp
Kiyokawa
Yasushi
Y
Takeuchi
Yukari
Y
Mori
Yuji
Y
D000280:Administration, Inhalation; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000139:chemically induced*; D002065:Buspirone / Q000494:pharmacology; D003000:Clonidine / Q000494:pharmacology; D004195:Disease Models, Animal; D008297:Male; D008874:Midazolam / Q000494:pharmacology; D010624:Phenelzine / Q000494:pharmacology; D010675:Pheromones / Q000008:administration & dosage* / Q000037:antagonists & inhibitors / Q000502:physiology; D011433:Propranolol / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013216:Reflex, Startle / Q000187:drug effects*
D014151:Anti-Anxiety Agents; C101628:CP 154526; D010675:Pheromones; D011743:Pyrimidines; D011758:Pyrroles; D011433:Propranolol; D003000:Clonidine; D010624:Phenelzine; D008874:Midazolam; D002065:Buspirone