Validation of double digest selective label database for sequenced prokaryotic genomes.
CONCLUSIONS
A database for simulation of double digest selective label (DDSL) typing technique has been created and validated against a sequenced strain (Salmonella enterica serovar Typhimurium strain LT2). In silico bands were in agreement with experimental, and the technique was able to discriminate among strains belonging to the same species. When compared with other strain discrimination techniques, DDSL showed a higher discriminatory power. The database contains precomputed data which may be searched to retrieve experimental conditions for typing all up-to-dated sequenced prokaryotic microorganisms.


BACKGROUND
This is a new resource for molecular biology freely available on the Internet at http://insilico.ehu.es/DDSL.
Terletskiy
Valery
V
All Russian Research Institute of Farm Animal Genetics and Breeding, Moscowskoye sh. 55a, 196625, St. Petersburg-Pushkin, Russia.
Tyshchenko
Valentina
V
Martinez-Ballesteros
Ilargi
I
Garaizar
Javier
J
Bikandi
Joseba
J
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry; D030541:Databases, Genetic; D016680:Genome, Bacterial; D012486:Salmonella typhimurium / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial