Salinity induces carbohydrate accumulation and sugar-regulated starch biosynthetic genes in tomato (Solanum lycopersicum L. cv. 'Micro-Tom') fruits in an ABA- and osmotic stress-independent manner.
Salinity stress enhances sugar accumulation in tomato (Solanum lycopersicum) fruits. To elucidate the mechanisms underlying this phenomenon, the transport of carbohydrates into tomato fruits and the regulation of starch synthesis during fruit development in tomato plants cv. 'Micro-Tom' exposed to high levels of salinity stress were examined. Growth with 160 mM NaCl doubled starch accumulation in tomato fruits compared to control plants during the early stages of development, and soluble sugars increased as the fruit matured. Tracer analysis with (13)C confirmed that elevated carbohydrate accumulation in fruits exposed to salinity stress was confined to the early development stages and did not occur after ripening. Salinity stress also up-regulated sucrose transporter expression in source leaves and increased activity of ADP-glucose pyrophosphorylase (AGPase) in fruits during the early development stages. The results indicate that salinity stress enhanced carbohydrate accumulation as starch during the early development stages and it is responsible for the increase in soluble sugars in ripe fruit. Quantitative RT-PCR analyses of salinity-stressed plants showed that the AGPase-encoding genes, AgpL1 and AgpS1 were up-regulated in developing fruits, and AgpL1 was obviously up-regulated by sugar at the transcriptional level but not by abscisic acid and osmotic stress. These results indicate AgpL1 and AgpS1 are involved in the promotion of starch biosynthesis under the salinity stress in ABA- and osmotic stress-independent manners. These two genes are differentially regulated at the transcriptional level, and AgpL1 is suggested to play a regulatory role in this event.
Yin
Yong-Gen
YG
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, Japan.
Kobayashi
Yoshie
Y
Sanuki
Atsuko
A
Kondo
Satoru
S
Fukuda
Naoya
N
Ezura
Hiroshi
H
Sugaya
Sumiko
S
Matsukura
Chiaki
C
D000040:Abscisic Acid / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*; D013213:Starch / Q000096:biosynthesis*; D013312:Stress, Physiological
D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D013213:Starch; D051599:Glucose-1-Phosphate Adenylyltransferase