title: [Comparison of modified Bethesda assay and Nijmegen assay in detecting FVII inhibitor in patients with hemophilia A].
abstract: OBJECTIVE
  To compare the sensitivity and practicability of modified Bethesda assay and Nijmegen assay in detecting factor VIII (FVIII) inhibitor.
  
  
  METHODS
  Modified Bethesda assay and Nijmegen assay were used to screen FVIII inhibitors in 237 patients with hemophilia A. The buffer plus universal coagulation reference plasma (UCRP) was used to establish a standard curve for FVIII: C assay in modified Bethesda method, instead of Nijmegen plasma plus FVIII deficiency plasma in Nijmegen method. The cutoff value for positive FVIII inhibitors is > or = 0.6 BU/ml.
  
  
  RESULTS
  The positive rate of FVIII inhibitors was 5.5% (n = 13) when using modified Bethesda assay and was 8.4% (n = 20) when using Nijmegen assay (P > 0.05).
  
  
  CONCLUSIONS
  Modified standard Bethesda assay is a convenient and feasible method for detecting FVIII inhibitors.
authors.affiliation: Department of Hematology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Lian-Kai
authors.initials: LK
authors.lastname: Fan
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: Bao-Lai
authors.initials: BL
authors.lastname: Hua
authors.forename: Wei
authors.initials: W
authors.lastname: Su
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Wang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001780:Blood Coagulation Tests / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D005169:Factor VIII