Small interfering RNA (siRNA) against the Bcl-2 gene increases apoptosis in a canine melanoma cell line.
The effects of down-regulation of Bcl-2 expression, by small interfering RNA (siRNA) against the canine Bcl-2 genes, on apoptosis were investigated by transfecting MCM-N1 (canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine Bcl-2 genes increased the number of apoptotic cells. In addition, sequence-specific down-regulation of Bcl-2 expression was measured by RT-PCR and western blot analysis. The siRNA directed against these genes reduced both mRNA and protein expression in the MCM-N1 cells. Our study suggests the importance of Bcl-2 in canine melanoma tumors for inducing apoptosis and reinforces using Bcl-2 as a putative therapeutic target in canine malignant melanoma tumor.
Watanabe
Yuzuru
Y
Department of Pathobiology, Nihon University School of Veterinary Medicine 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Kano
Rui
R
Maruyama
Haruhiko
H
Hasegawa
Atsuhiko
A
Kamata
Hiroshi
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D019254:Genes, bcl-2 / Q000187:drug effects / Q000235:genetics*; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D034741:RNA, Small Interfering / Q000627:therapeutic use*; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering