Optimization of capsular polysaccharide production by Streptococcus pneumoniae type 3.
Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were designed to assess fermentation pH, supplemented glucose concentration, and stirring rate. The predicted highest CPS production by the obtained optimization model equation was 256.14 mg/l at optimal conditions [pH 7.5, stirring rate 180 rpm, and supplemented glucose concentration 1% (w/v)]. The validity of the response model was confirmed by the good agreement between the predicted and experimental results. The maximum amount of CPS obtained was 255.03+/-2.23 mg/l.
Jin
Sheng-De
SD
School of Biological Sciences and Technology, Chonnam National University, Gwang-ju 500-757, Korea.
Kim
Young-Min
YM
Kang
Hee-Kyoung
HK
Jung
Seung-Jin
SJ
Kim
Doman
D
D016667:Bacterial Capsules / Q000096:biosynthesis* / Q000302:isolation & purification; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008954:Models, Biological; D015233:Models, Statistical; D011008:Pneumococcal Infections / Q000382:microbiology / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000627:therapeutic use; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D022242:Pneumococcal Vaccines; D005947:Glucose