title: Small interfering RNA (siRNA) against the Bcl-2 gene increases apoptosis in a canine melanoma cell line.
abstract: The effects of down-regulation of Bcl-2 expression, by small interfering RNA (siRNA) against the canine Bcl-2 genes, on apoptosis were investigated by transfecting MCM-N1 (canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine Bcl-2 genes increased the number of apoptotic cells. In addition, sequence-specific down-regulation of Bcl-2 expression was measured by RT-PCR and western blot analysis. The siRNA directed against these genes reduced both mRNA and protein expression in the MCM-N1 cells. Our study suggests the importance of Bcl-2 in canine melanoma tumors for inducing apoptosis and reinforces using Bcl-2 as a putative therapeutic target in canine malignant melanoma tumor.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Maruyama
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D019254:Genes, bcl-2 / Q000187:drug effects / Q000235:genetics*; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D034741:RNA, Small Interfering / Q000627:therapeutic use*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering