Chemical composition and in vitro antimicrobial activity of the volatile oils from Gliomastix murorum and Pichia guilliermondii, two endophytic fungi in Paris polyphylla var. yunnanensis.
Volatile oils were obtained by hydro-distillation from Gliomastix murorum and Pichia guilliermondii, two endophytic fungi isolated from the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis. The oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (GC-MS). Palmitic acid (15.5%), (E)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7Z,10Z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identified components in G. murorum volatile oil. 1,1,3a,7-Tetramethyl-1a,2,3,3a,4,5,6,7b-octahydro-1H-cyclopropa[a]- naphthalene (25.9%), palmitic acid (15.5%), 1-methyl-2,4-di- (prop-1-en-2-yl)-1- vinylcyclohexane (7.9%), (E)-9-octadecenoic acid (7.3%), and (9E,12E)-ethyl-9,12-octadecadienoate (5.2%) were the major compounds of the 27 identified components in P. guilliermondii volatile oil. The in vitro antimicrobial activity of the volatile oils was also investigated to evaluate their efficacy against six bacteria and one phytopathogenic fungus. The minimum inhibitory concentration (MIC) values of the volatile oils against the test bacteria ranged from 0.20 mg/mL to 1.50 mg/mL. One of the most sensitive bacteria was Xanthomonas vesicatoria with an MIC of 0.20 mg/mL and 0.40 mg/mL for G. murorum and P. guilliermondii, respectively. The mean inhibitory concentration (IC50) of the volatile oils against spore germination of Magnaporthe oryzae was 0.84 mg/mL for G. murorum and 1.56 mg/mL for P. guilliermondii. These results indicated that the volatile oils from the endophytic fungi have strong antimicrobial activity and could be a potential source of antimicrobial ingredients.
Zhao
Jianglin
J
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Shan
Tijiang
T
Huang
Yongfu
Y
Liu
Xili
X
Gao
Xiwu
X
Wang
Mingan
M
Jiang
Weibo
W
Zhou
Ligang
L
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D020082:Magnaporthe / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry*; D010843:Pichia / Q000737:chemistry*; D010944:Plants / Q000382:microbiology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development
D000890:Anti-Infective Agents; D009822:Oils, Volatile