title: Towards characterization of the chloroplast NAD(P)H dehydrogenase complex.
abstract: The NAD(P)H dehydrogenase (NDH) complex in chloroplast thylakoid membranes functions in cyclic electron transfer, and in chlororespiration. NDH is composed of at least 15 subunits, including both chloroplast- and nuclear-encoded proteins. During the past few years, extensive proteomic and genetic research on the higher plant NDH complex has been carried out, resulting in identification of several novel nuclear-encoded subunits. In addition, a number of auxiliary proteins, which mainly regulate the expression of chloroplast-encoded ndh genes as well as the assembly and stabilization of the NDH complex, have been discovered and characterized. In the absence of detailed crystallographic data, the structure of the NDH complex has remained obscure, and therefore the role of several NDH-associated nuclear-encoded proteins either as auxiliary proteins or structural subunits remains uncertain. In this review, we summarize the current knowledge on the subunit composition and assembly process of the chloroplast NDH complex. In addition, a novel oligomeric structure of NDH, the PSI/NDH supercomplex, is discussed.
authors.affiliation: Department of Biology, Plant Physiology and Molecular Biology, University of Turku, FI-20014 Turku, Finland.
authors.forename: Marjaana
authors.initials: M
authors.lastname: Suorsa
authors.forename: Sari
authors.initials: S
authors.lastname: Sirpiö
authors.forename: Eva-Mari
authors.initials: EM
authors.lastname: Aro
mesh_terms: D002736:Chloroplasts / Q000201:enzymology*; D014644:Genetic Variation; D008970:Molecular Weight; D009252:NADPH Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D020524:Thylakoids / Q000201:enzymology
chemical_list: D021122:Protein Subunits; D009252:NADPH Dehydrogenase