Herbivore response in passion fruit (Passiflora edulis Sims) plants: induction of lipoxygenase activity in leaf tissue in response to generalist and specialist insect attack.
Lipoxygenases (LOXs, EC 1.13.11.12) are a class of non-heme iron containing dioxygenases which catalyze the regiospecific and stereospecific hydroperoxidation of polyunsaturated fatty acids with 1,4-pentadiene system such as linoleic acid and linolenic acid in plants. In this work we studied the LOX activity in damaged as well as in distal leaves in response to specialist (Agraulis vanillae vanillae) or generalist (Spodoptera frugiperda) insect attack. Enzymatic assays showed that induction of LOX activity occurred locally and systemically in response to both insects' attacks. Northern blot analysis revealed that LOX expression is also insect-inducible in agreement with enzymatic assay results. In addition, northern analysis corroborated previous reports that LOX activity is wound- and methyl jasmonate-inducible. These results suggest that the herbivore-response in passion fruit is mediated by jasmonates, since a key enzyme of the biosynthetic pathway of jasmonic acid is induced upon lepidopteran insects' attacks.
Jardim
Bruno C
BC
Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-600, Campos dos Goytacazes, RJ, Brazil.
Perdïzio
Viviane A
VA
Berbert-Molina
Marïlia A
MA
Rodrigues
Deivid C
DC
Botelho-Júnior
Sylvio
S
Vicente
Ana C P
AC
Hansen
Ekkehard
E
Otsuki
Koko
K
Urmënyi
Turán P
TP
Jacinto
Tânia
T
D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D018506:Gene Expression Regulation, Plant; D007915:Lepidoptera / Q000502:physiology*; D008084:Lipoxygenase / Q000096:biosynthesis* / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D029598:Passiflora / Q000201:enzymology* / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology; D018749:RNA, Plant / Q000378:metabolism
D003517:Cyclopentanes; D054883:Oxylipins; D018749:RNA, Plant; C011006:jasmonic acid; D008084:Lipoxygenase