Disease-specific gene repositioning in breast cancer.
Genomes are nonrandomly organized within the three-dimensional space of the cell nucleus. Here, we have identified several genes whose nuclear positions are altered in human invasive breast cancer compared with normal breast tissue. The changes in positioning are gene specific and are not a reflection of genomic instability within the cancer tissue. Repositioning events are specific to cancer and do not generally occur in noncancerous breast disease. Moreover, we show that the spatial positions of genes are highly consistent between individuals. Our data indicate that cancer cells have disease-specific gene distributions. These interphase gene positioning patterns may be used to identify cancer tissues.
Meaburn
Karen J
KJ
National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Gudla
Prabhakar R
PR
Khan
Sameena
S
Lockett
Stephen J
SJ
Misteli
Tom
T
D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010641:Phenotype; D011237:Predictive Value of Tests; D055815:Young Adult
D014408:Biomarkers, Tumor