Identification of carotenoids from green alga Haematococcus pluvialis by HPLC and LC-MS (APCI) and their antioxidant properties.
Haematococcus pluvialis, a green alga accumulates astaxathin upto 2-3% on dry weight basis. In the present study, identification of carotenoids from Haematococcus cyst cell extract by HPLC and LC-MS (APCI) and their antioxidant properties were evaluated in vitro model systems. The extract exhibited 89% and 78% antioxidant activity in beta-carotene linoleate model, and hydroxyl radical scavenging model at 9 ppm of total carotenoid respectively. The extract also showed 80%, 85% and 79% antioxidant activity against lipid peroxidation in kidney, brain and liver of rats. Low-density lipoprotein oxidation induced by Cu2+ ions also protected (45%, 64% and 75%) by the extract in a dose dependent manner with different carotenoid levels. Thiobarbituric acid reactive substances concentration in the blood, liver, and kidney of rat was also significantly (p<0.005) decreased in H. pluvialis treated rats. Potent antioxidant activity is attributable to various carotenoids present in the extract.
Ranga
Rao
R
Biochemistry and Nutrition Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research), Mysore-570 020, Karnataka, India.
Sarada
A Ravi
AR
Baskaran
V
V
Ravishankar
G A
GA
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D016823:Volvocida / Q000737:chemistry*; D024341:Xanthophylls / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D019207:beta Carotene / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D024341:Xanthophylls; D019207:beta Carotene