Pivotal role of curcuminoids on the antimutagenic activity of Curcuma zedoaria extracts.
In an investigation of the mutagenic activity of two extracts from rhizomes of Curcuma zedoaria (the mathanolic, CME, and the aqueous, CAE) and antimutagenic activity against mutagens, either 2-amino-3-methylimidazo (4,5-f) quinoline (IQ) or 4-nitroquinoline-N-oxide (4-NQO), in dosages of 1-50 microg/plate was assayed by using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains. We found that the two extracts showed no mutagenicity when tested with all the tester strains either with or without the S9 mix. Moreover, the two extracts, particularly CME, presented a greater antimutagenicity than CAE did either in IQ or 4-NQO mutagens. However, the inhibition effect on lipid peroxidation was similar with both extracts. The amount of major antioxidants beta-carotene, ascorbic acid, and total polyphenols present in both CME and CAE were similar between each other. In contrast, the content of cucuminoids (44.3 mg/g extract) in CME were only found in small amounts in CAE (0.09 mg/g extract). Consequently, although both of the extracts showed similar antioxidant effects, the curcuminoids, which seem to be the main active principles from this plant, were suggested to play a pivotal role in antimutagenic activity.
Peng
Chiung-Huei
CH
Research Institute of Biotechnology, Hungkuang University, Taichung Hsien, Taiwan, ROC.
Chiu
Wen-Ta
WT
Juan
Chi-Wen
CW
Mau
Jeng-Leun
JL
Chen
Chien-Chou
CC
Peng
Chiung-Chi
CC
Lai
Eric-Yc
EY
Chyau
Charng-Cherng
CC
D016587:Antimutagenic Agents / Q000494:pharmacology*; D030024:Curcuma / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols; D011804:Quinolines / Q000494:pharmacology; D012486:Salmonella typhimurium; D019207:beta Carotene / Q000494:pharmacology*
D016587:Antimutagenic Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D011804:Quinolines; D019207:beta Carotene; C037219:quinoline