title: The effects of hemodilution on acute inflammatory responses in a bleomycin-induced lung injury model.
abstract: Acute normovolemic hemodilution (ANH) can be used in acute lung injury (ALI) patients who refuse blood transfusions. To investigate the effects of hemodilution on the acute inflammatory response in lung injury, the authors studied the effects of ANH in a rat model of bleomycin-induced lung injury. Bleomycin (10 mg/kg) was used to induce lung injury in 2 groups of rats. The treatment groups included a lung injury group with hemodilution (HI), a lung injury group without hemodilution (NHI), and a control group. Hemodilution was performed by removing blood and substituting the same amount of hydroxyethyl starch solution targeted to 7.0 g/dL via the right and left internal jugular veins. At day 3 after bleomycin instillation, systemic hemoglobin concentration was 9.5 +/- 1.1 g/dL. Tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 levels measured in the bronchoalveolar lavage fluid (BALF), blood, and lung tissue were not significantly different between the HI and NHI groups 3 days after lung injury. Microscopic findings showed fibrosis and inflammation in the HI and NHI groups 28 days after lung injury, but no significant differences were found between the 2 groups. Hemodilution after bleomycin administration did not further affect the acute inflammatory response or lung injury.
authors.affiliation: Department of Anesthesia and Pain Medicine, Paik Hospital, Inje University, Busan, Republic of Korea. anesjsh@medimail.co.kr
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Cheong
authors.forename: Jeong Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Kun Moo
authors.initials: KM
authors.lastname: Lee
authors.forename: Kwang Rae
authors.initials: KR
authors.lastname: Cho
authors.forename: Young Il
authors.initials: YI
authors.lastname: Yang
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Seo
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Yoon
authors.forename: Jeong Nyeo
authors.initials: JN
authors.lastname: Lee
authors.forename: Min Young
authors.initials: MY
authors.lastname: Choi
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Lim
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000740:Anemia / Q000097:blood / Q000150:complications / Q000628:therapy; D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D001804:Blood Transfusion, Autologous; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D006438:Hemodilution / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D001761:Bleomycin