title: Stages in tropoelastin coalescence during synthetic elastin hydrogel formation.
abstract: Synthetic human tropoelastin was chemically cross-linked to form elastic hydrogel-like structures in vitro. Discrete stages were identified during elastic hydrogel formation by cross-linking tropoelastin with bis(sulfosuccinimidyl) suberate at a range of protein concentrations during this process. In the early stages of this process, particles with the same dimensions as tropoelastin were seen. As hydrogel formation progressed, monomer width fibres were also observed. Overall, four distinct stages were identified: (1) tropoelastin monomers form discrete particles in the order of 200 nm diameter, (2) these particles merge to form larger spheres, (3) spheres coalesce into open linked networks, (4) coalesced spheres consolidate to form a porous structure to give synthetic elastin hydrogels.
authors.affiliation: School of Molecular and Microbial Biosciences G08, University of Sydney, NSW 2006, Australia.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Tu
authors.forename: Steven G
authors.initials: SG
authors.lastname: Wise
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D004549:Elastin / Q000378:metabolism*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000378:metabolism; D014333:Tropoelastin / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014333:Tropoelastin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004549:Elastin