title: Disease-specific gene repositioning in breast cancer.
abstract: Genomes are nonrandomly organized within the three-dimensional space of the cell nucleus. Here, we have identified several genes whose nuclear positions are altered in human invasive breast cancer compared with normal breast tissue. The changes in positioning are gene specific and are not a reflection of genomic instability within the cancer tissue. Repositioning events are specific to cancer and do not generally occur in noncancerous breast disease. Moreover, we show that the spatial positions of genes are highly consistent between individuals. Our data indicate that cancer cells have disease-specific gene distributions. These interphase gene positioning patterns may be used to identify cancer tissues.
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Meaburn
authors.forename: Prabhakar R
authors.initials: PR
authors.lastname: Gudla
authors.forename: Sameena
authors.initials: S
authors.lastname: Khan
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lockett
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010641:Phenotype; D011237:Predictive Value of Tests; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor