The alpha glycerophosphate cycle in Drosophila melanogaster V. molecular analysis of alpha glycerophosphate dehydrogenase and alpha glycerophosphate oxidase mutants.
Two enzymes, alpha glycerophosphate dehydrogenase (GPDH-1) in the cytoplasm and alpha glycerophosphate oxidase (GPO-1) in the mitochondrion cooperate in Drosophila flight muscles to generate the ATP needed for muscle contraction. Null mutants for either enzyme cannot fly. Here, we characterize 15 ethyl methane sulfonate (EMS)-induced mutants in GPDH-1 at the molecular level and assess their effects on structural and evolutionarily conserved domains of this enzyme. In addition, we molecularly characterize 3 EMS-induced GPO-1 mutants and excisions of a P element insertion in the GPO-1 gene. The latter represent the best candidate for null or amorphic mutants in this gene.
Carmon
Amber
A
Department of Molecular Biology and Genetics, 407 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA.
Chien
Jeff
J
Sullivan
David
D
MacIntyre
Ross
R
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D004789:Enzyme Activation / Q000235:genetics; D005426:Flight, Animal / Q000502:physiology; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics
D005994:Glycerophosphates; D050505:Mutant Proteins; C029620:alpha-glycerophosphoric acid; D005993:Glycerolphosphate Dehydrogenase