Alleviation effect of alginate-derived oligosaccharides on Vicia faba root tip cells damaged by cadmium.
Cadmium has been shown to prevent Vicia faba growth by inhibiting cell mitosis. In this study we investigated the role of Alginate-derived Oligosaccharides (ADO) in alleviating Vicia faba root tip cells damaged by 6 and 8 mg L(-1) CdCl2. Micronucleus assay and chromosomal aberration assay were used to determine mitotic index, micronucleus frequency and chromosomal aberration frequency. The results showed that micronucleus frequency of Vicia faba root tip cells was inhibited under all the ADO concentrations. Especially, the inhibition ratio of 0.125% ADO highly reached 66.11 and 67.17% in 6 and 8 mg L(-1) CdCl2, respectively. Furthermore, the mitotic index increased (p < 0.05) and chromosomal aberration frequency decreased (p < 0.05) under all the ADO concentrations. This indicated that ADO had a significant alleviation effect on Vicia faba root tip cells damaged by cadmium.
Ma
L J
LJ
College of Chemistry and Life Science, Shenyang Normal University, 253 Huanghe Street, 110034 Shenyang, China. malianju@tom.com
Zhang
Y
Y
Bu
N
N
Wang
S H
SH
D000464:Alginates / Q000494:pharmacology*; D002105:Cadmium Poisoning / Q000188:drug therapy* / Q000473:pathology*; D002869:Chromosome Aberrations / Q000187:drug effects; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D009844:Oligosaccharides / Q000494:pharmacology*; D010935:Plant Diseases; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects; D012639:Seeds / Q000254:growth & development; D031307:Vicia faba / Q000502:physiology*
D000464:Alginates; D009844:Oligosaccharides