Extensive functional diversification of the Populus glutathione S-transferase supergene family.
Identifying how genes and their functions evolve after duplication is central to understanding gene family radiation. In this study, we systematically examined the functional diversification of the glutathione S-transferase (GST) gene family in Populus trichocarpa by integrating phylogeny, expression, substrate specificity, and enzyme kinetic data. GSTs are ubiquitous proteins in plants that play important roles in stress tolerance and detoxification metabolism. Genome annotation identified 81 GST genes in Populus that were divided into eight classes with distinct divergence in their evolutionary rate, gene structure, expression responses to abiotic stressors, and enzymatic properties of encoded proteins. In addition, when all the functional parameters were examined, clear divergence was observed within tandem clusters and between paralogous gene pairs, suggesting that subfunctionalization has taken place among duplicate genes. The two domains of GST proteins appear to have evolved under differential selective pressures. The C-terminal domain seems to have been subject to more relaxed functional constraints or divergent directional selection, which may have allowed rapid changes in substrate specificity, affinity, and activity, while maintaining the primary function of the enzyme. Our findings shed light on mechanisms that facilitate the retention of duplicate genes, which can result in a large gene family with a broad substrate spectrum and a wide range of reactivity toward different substrates.
Lan
Ting
T
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Yang
Zhi-Ling
ZL
Yang
Xue
X
Liu
Yan-Jing
YJ
Wang
Xiao-Ru
XR
Zeng
Qing-Yin
QY
D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D020131:Genes, Duplicate; D017343:Genes, Plant; D005982:Glutathione Transferase / Q000235:genetics*; D008958:Models, Molecular; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological; D013379:Substrate Specificity
D018744:DNA, Plant; D010940:Plant Proteins; D005982:Glutathione Transferase