Improved detection of microbial DNA after bead-beating before DNA isolation.
Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.
de Boer
Richard
R
Department of Research & Development, Laboratory for Infectious Diseases Groningen, The Netherlands.
Peters
Remco
R
Gierveld
Sonja
S
Schuurman
Tim
T
Kooistra-Smid
Mirjam
M
Savelkoul
Paul
P
D001433:Bacteriolysis; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis*; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods
D004269:DNA, Bacterial