title: Evaluation of IgM antibody capture enzyme-linked immunosorbent assay kits for detection of IgM against Japanese encephalitis virus in cerebrospinal fluid samples.
abstract: Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEV-specific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65-80% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs.
authors.affiliation: Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
authors.forename: Vasanthapuram
authors.initials: V
authors.lastname: Ravi
authors.forename: Jaimie S
authors.initials: JS
authors.lastname: Robinson
authors.forename: Brandy J
authors.initials: BJ
authors.lastname: Russell
authors.forename: Anita
authors.initials: A
authors.lastname: Desai
authors.forename: Nalini
authors.initials: N
authors.lastname: Ramamurty
authors.forename: David
authors.initials: D
authors.lastname: Featherstone
authors.forename: Barbara W
authors.initials: BW
authors.lastname: Johnson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D055815:Young Adult
chemical_list: D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic