[FLT3 mutation in patients with acute lymphoblastic leukemia and its clinical significance].
OBJECTIVE
To investigate fms-like tyrosine kinase 3 (FLT3) mutation in patients with acute lymphoblastic leukemia (ALL) and its clinical significance.


METHODS
FLT3 mutation-internal tandem duplication (FLT3-ITD) and FLT3 mutation in the tyrosine kinase domain (FLT3-TKD) were detected using polymerase chain reaction (PCR) in the genomic DNA of 61 ALL patients and 7 healthy volunteers (as reference group). The PCR products of these patients who were detected with FLT3-ITD or FLT3-TKD were sent for sequence analysis.


RESULTS
In all these 61 ALL patients, FLT3-ITDs were identified in 2 patients and FLT3-TKD were identified in one patient.


CONCLUSIONS
FLT3-ITD and FLT3-TKD exist in a small proportion of ALL patients.
Zhao
Xin
X
State Key Laboratory of Experimental Hematology, Department of Hematologic Oncology, Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Tang
Ke-Jing
KJ
Tian
Zheng
Z
Chen
Li-Ping
LP
Mi
Ying-Chang
YC
Wang
Jian-Xiang
JX
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D055815:Young Adult; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics*
C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3