Porcine reproductive and respiratory syndrome virus attachment is mediated by the N-terminal domain of the sialoadhesin receptor.
Sialoadhesin (Sn) is an important receptor for viral attachment and internalization of porcine reproductive and respiratory syndrome virus (PRRSV) to porcine alveolar macrophages (PAM). To investigate whether the N-terminal domain of Sn is sufficient and/or necessary for PRRSV attachment, we constructed a series of truncated fragments of porcine Sn and expressed these in the non-permissive PK15 cell line. The first 150 amino acids comprising the entire first domain of the Sn N-terminal region was necessary for PRRSV binding to cells, and the N-terminal domain alone was sufficient for virus attachment. The attachment of PRRSV to PAM cells was inhibited by polyclonal anti-serum against the N-terminal region of porcine Sn in a dose-dependent manner. The present study demonstrates that the first domain at the N-terminus of Sn mediates PRRSV attachment to PAM cells and contributes to better understanding the interaction between PRRSV and its host cells.
An
Tong-Qing
TQ
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Tian
Zhi-Jun
ZJ
He
Yun-Xia
YX
Xiao
Yan
Y
Jiang
Yi-Feng
YF
Peng
Jin-Mei
JM
Zhou
Yan-Jun
YJ
Liu
Di
D
Tong
Guang-Zhi
GZ
D000818:Animals; D002478:Cells, Cultured; D050660:Core Binding Factors; D017353:Gene Deletion; D016676:Macrophages, Alveolar / Q000502:physiology / Q000821:virology*; D008562:Membrane Glycoproteins / Q000502:physiology*; D008958:Models, Molecular; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011485:Protein Binding; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000502:physiology*; D063267:Sialic Acid Binding Ig-like Lectin 1; D013552:Swine; D053585:Virus Attachment
D050660:Core Binding Factors; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D063267:Sialic Acid Binding Ig-like Lectin 1