title: Intermediate rate atomic trajectories of RNA by solid-state NMR spectroscopy.
abstract: Many RNAs undergo large conformational changes in response to the binding of proteins and small molecules. However, when RNA functional dynamics occur in the nanosecond-microsecond time scale, they become invisible to traditional solution NMR relaxation methods. Residual dipolar coupling methods have revealed the presence of extensive nanosecond-microsecond domain motions in HIV-1 TAR RNA, but this technique lacks information on the rates of motions. We have used solid-state deuterium NMR to quantitatively describe trajectories of key residues in TAR by exploiting the sensitivity of this technique to motions that occur in the nanosecond-microsecond regime. Deuterium line shape and relaxation data were used to model motions of residues within the TAR binding interface. The resulting motional models indicate two functionally essential bases within the single-stranded bulge sample both the free and Tat-bound conformations on the microsecond time scale in the complete absence of the protein. Thus, our results strongly support a conformational capture mechanism for recognition: the protein does not induce a new RNA structure, but instead captures an already-populated conformation.
authors.affiliation: Department of Chemistry, Box 351700, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Greg L
authors.initials: GL
authors.lastname: Olsen
authors.forename: Michael F
authors.initials: MF
authors.lastname: Bardaro
authors.forename: Dorothy C
authors.initials: DC
authors.lastname: Echodu
authors.forename: Gary P
authors.initials: GP
authors.lastname: Drobny
authors.forename: Gabriele
authors.initials: G
authors.lastname: Varani
mesh_terms: D015497:HIV-1; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009068:Movement; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D013997:Time Factors
chemical_list: D012367:RNA, Viral; D012996:Solutions