title: "The heat is on": rapid microcalorimetric detection of mycobacteria in culture.
abstract: Detection of mycobacterial infection can be achieved by different means; however, culture-based methods remain the gold standard. In this paper, we present a new culture-based method using real-time microcalorimetric detection of growth of Mycobacterium species including Mycobacterium tuberculosis. Microcalorimetric detection of heat production by 6 different growing species of Mycobacterium was achieved between 20 and 310h depending on their type (fast vs. slow-growing mycobacteria) and initial concentration. This study demonstrates that microcalorimetric detection of mycobacterial growth is a potential advantageous alternative to methods using fluorescent or radiolabeled growth indicators.
authors.affiliation: Lab. of Biomechanics and Biocalorimetry (LOB(2)), Faculty of Medicine, University of Basel, c/o Biozentrum - Pharmazentrum, Klingelbergstrasse 50-70, 4056 Basel, Switzerland. Olivier.braissant@unibas.ch <Olivier.braissant@unibas.ch>
authors.forename: O
authors.initials: O
authors.lastname: Braissant
authors.forename: D
authors.initials: D
authors.lastname: Wirz
authors.forename: B
authors.initials: B
authors.lastname: Göpfert
authors.forename: A U
authors.initials: AU
authors.lastname: Daniels
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002151:Calorimetry / Q000379:methods; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000302:isolation & purification; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D003470:Culture Media