title: Seven consecutive successful clinical islet isolations with pancreatic ductal injection.
abstract: Inconsistent islet isolation is one of the issues of clinical islet transplantation. In the current study, we applied ductal injection to improve the consistency of islet isolation. Seven islet isolations were performed with the ductal injection of ET-Kyoto solution (DI group) and eight islet isolations were performed without the ductal injection (standard group) using brain-dead donor pancreata. Isolated islets were evaluated based on the Edmonton protocol for transplantation. The DI group had significantly higher islet yields (588,566 +/- 64,319 vs. 354,836 +/- 89,649 IE, p < 0.01) and viability (97.3 +/- 1.2% vs. 92.6 +/- 1.2%, p < 0.02) compared with the standard group. All seven isolated islet preparations in the DI group (100%), versus only three out of eight isolated islet preparations (38%) in the standard group met transplantation criteria. The islets from the DI group were transplanted into three type 1 diabetic patients and all three patients became insulin independent. Ductal injection significantly improved quantity and quality of isolated islets and resulted in high success rate of clinical islet transplantation. This simple modification will reduce the risk of failure of clinical islet isolation.
authors.affiliation: Baylor Research Institute, Fort Worth, TX 76104, USA. shinichm@baylorhealth.edu
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguichi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimoda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikemoto
authors.forename: Bashoo
authors.initials: B
authors.lastname: Naziruddin
authors.forename: Andrew
authors.initials: A
authors.lastname: Jackson
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Tamura
authors.forename: Greg
authors.initials: G
authors.lastname: Olson
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Fujita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Chujo
authors.forename: Morihito
authors.initials: M
authors.lastname: Takita
authors.forename: Naoya
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Nicholas
authors.initials: N
authors.lastname: Onaca
authors.forename: Marlon
authors.initials: M
authors.lastname: Levy
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery*; D004210:Dissection / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D007267:Injections / Q000379:methods; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods* / Q000592:standards; D008297:Male; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards; D016896:Treatment Outcome