[Experimental study on hemostasis of a new chitosan hemostatic powder].
OBJECTIVE
To study hemostasis of a new chitosan hemostatic powder.


METHODS
Twenty-four adult SD rats were made the models of liver injury, male or female, and weighing 210-240 g. They were divided into three groups randomly (n = 8) depending on different hemostatic powders. The incision of the liver was treated with 300 mg Yunnan baiyao (group A1), chitosan hemostatic powder of pH 6.5 (group B1) and pH 7.5 (group C1), respectively. The bleeding time and bleeding amount were recorded. In vitro, with the modified Ree-White method, 2 mL artery blood from New Zealand white rabbit was added into the 0.2 mL solution of Yunnan baiyao, chitosan hemostatic powder of pH 6.5 and pH 7.5 (concentration of 0.2 mg/mL), respectively. The blood coagulation time was recorded. The chitosan blood clots of group B2 and group C2 were observed with scanning electron microscopy (SEM).


RESULTS
The bleeding time of group A1, group B1 and group C1 was (292 +/- 31), (261 +/- 23), and (224 +/- 28) s, respectively, the bleeding amount was (1.63 +/- 0.21), (1.47 +/- 0.18), and (1.18 +/- 0.17) g, respectively, showing statistically significant differences between groups B1, C1, and group A1 (P < 0.05), between group C1 and group B1 (P < 0.05). The blood clotting time of group A2, group B2, and group C2 was (653 +/- 41), (255 +/- 20), and (202 +/- 11) s, respectively, showing statistically significant differences between groups B2, C2, and group A2 (P < 0.05), between group C2 and group B2 (P < 0.05). The SEM showed that the blood cells of group B2 and group C2 gathered around the chitosan.


CONCLUSIONS
Chitosan hemostatic powder of pH 7.5 has good hemostasis.
Yin
Gang
G
Department of Orthopaedics, Changzheng Hospital of the Second Military Medical University, Shanghai 200003, PR China.
Hou
Chunlin
C
Liu
Wanshun
W
Han
Baoqin
B
Wei
Changzheng
C
Liu
Shuangli
S
Guo
Xingfeng
X
Yu
Bing
B
D000818:Animals; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D048271:Chitosan / Q000627:therapeutic use*; D005260:Female; D006490:Hemostatics / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D006490:Hemostatics; D048271:Chitosan