title: Simultaneous determination of cyclodol and diprazin by thin layer chromatography and high performance liquid chromatography.
abstract: Ciklodol (trihexyphenidil)--the central and peripheral m-cholinoblocker is currently used with other antipsychotic drugs such as phenotiazines and tricycle antidepressants. For the purpose of simultaneous determination of ciklodol and diprazine, were selected two methods of analysis: Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). During development of TLC method was studied the 10 visualizing system and 24 mobile systems. For individual or simultaneous determination of ciklodol and diprazine were recommended the following solvents' systems: 1. Toluene-acetone-ethanole-25%NH(4)OH (45:45: 7.5:2.5), 2. Hexane-ethyl acetate (15:5), 3. Chloroform-heptene-25%NH(4)OH (16:3:3), 4. Ethylacetate-hexane (10:10), 5. Acetonitrile-metanol (10:10) and 6.Heptene-chloroform-ethanol-25% NH(4)OH (5:10:3:1). As visualizing systems were chosen: Iodine vapors, blacklight (UV254) and reagent of FNP. Reagent of FNP gives colored spot just with diprazine and it is also could be used for separation of both objects in simultaneous analysis. Developed HPLC method of simultaneous determination of ciklodol and diprazine: like mobile phase is recommended: Acetonitril- 0.05M KH(2)PO4 (55:45) (v/v) +H(3)PO(4) (pH3.5), column EC250 x 4.6mm, with solid phase Nucleosil, flow rate 1ml/min, sample volume 40 microl. In given conditions, the retention time of ciklodol is 6.005min and diprazine 7.227min. Developed method of simultaneous determination and separation of ciklodol and diprazine in respective mixtures could be successfully applied as in the pharmaceutical, as well in the chemical-toxicological laboratories.
authors.affiliation: Tbilisi State Medical University, Department Pharmaceutical and Toxicological Chemistry.
authors.forename: R
authors.initials: R
authors.lastname: Makharadze
authors.forename: L
authors.initials: L
authors.lastname: Adeishvili
authors.forename: T
authors.initials: T
authors.lastname: Chelidze
authors.forename: N
authors.initials: N
authors.lastname: Imnadze
authors.forename: N
authors.initials: N
authors.lastname: Nizharadze
mesh_terms: D000978:Antiparkinson Agents / Q000191:economics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000191:economics; D002855:Chromatography, Thin Layer / Q000191:economics; D003362:Cost-Benefit Analysis; D006634:Histamine H1 Antagonists / Q000191:economics / Q000378:metabolism*; D006801:Humans; D011398:Promethazine / Q000191:economics / Q000378:metabolism*; D012015:Reference Standards; D014282:Trihexyphenidyl / Q000191:economics / Q000378:metabolism*
chemical_list: D000978:Antiparkinson Agents; D006634:Histamine H1 Antagonists; D014282:Trihexyphenidyl; D011398:Promethazine