Stable modification of poly(lactic acid) surface with neurite outgrowth-promoting peptides via hydrophobic collagen-like sequence.
Surface modification of poly(dl-lactic acid) (PLA) scaffolds has been performed using a biofunctional small peptide composed of collagen-like repetitive sequence and laminin-derived sequence (AG73-G(3)-(PPG)(5)) via hydrophobic interaction. The results of surface analysis suggest that AG73-G(3)-(PPG)(5) can be stably adsorbed onto PLA films via hydrophobic interaction at the (PPG)(5) region, and form an extracellular matrix-like layer composed of both structural and biosignalling sequences. In addition, neurite outgrowth of PC12 cells was observed on the AG73-G(3)-(PPG)(5)-adsorbed PLA film. These results indicate that AG73-G(3)-(PPG)(5) very effectively enhances neurite outgrowth activity on PLA films. The hydrophobic adsorption of collagen-like peptide bound to biosignalling molecules may be widely applied as a surface modifier of PLA films for tissue engineering.
Kakinoki
Sachiro
S
Department of Biomedical Engineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
Yamaoka
Tetsuji
T
D000595:Amino Acid Sequence; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D049108:Cell Enlargement; D002460:Cell Line; D005109:Extracellular Matrix / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry*; D008422:Materials Testing; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010455:Peptides / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D007797:Laminin; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)