title: Attachment of cultured fibroblasts and ultrastructural analysis of simulated cervical resorptions treated with high-power lasers and MTA.
abstract: OBJECTIVE
  The present study evaluated the fibroblast attachment and the morphologic changes of simulated cervical root resorptions after irradiation with high-power lasers and the use of mineral trioxide aggregate (MTA).
  
  
  METHODS
  Standardized cavities were done on the coronal third of 40 single-rooted teeth and assigned into 4 groups according to the treatment applied: (1) untreated control, (2) use of MTA, (3) irradiation with Er:YAG laser (42 mJ, 10 Hz, 10 s), and (4) irradiation with high-power diode laser (1 W, 10 s). Fragments were prepared for SEM analysis: 6 samples of each group were plated with human gingival fibroblasts and 4 samples were used for ultrastructural analysis.
  
  
  RESULTS
  The cells' attachment was in decreasing order: G3 > G4 > G1> G2, with statistical differences among all groups (P < .05). The samples irradiated with Er:YAG exhibited roughness on the dentinal surface, no smear layer, and open dentinal tubules; those irradiated with diode laser exhibited a smooth surface, more smear layer, and closed dentinal tubules; and the control group showed high amount of smear layer and closed dentin tubules.
  
  
  CONCLUSIONS
  Irradiation with Er:YAG and diode lasers caused morphologic changes on the dentinal surfaces of simulated resorptions that favored cells' adhesion. MTA showed lower biocompatibility than irradiated groups but allowed cells' adhesion.
authors.affiliation: Division of Endodontics, Department of Dentistry, University of Taubate, Taubate, Sao Paulo, Brazil. pontesdenise@uol.com.br
authors.forename: Denise Pontes
authors.initials: DP
authors.lastname: Raldi
authors.forename: Isabel
authors.initials: I
authors.lastname: Mello
authors.forename: Ana Christina Claro
authors.initials: AC
authors.lastname: Neves
authors.forename: Sandra Marcia
authors.initials: SM
authors.lastname: Habitante
authors.forename: Suely Sueli Patrícia
authors.initials: SS
authors.lastname: Miyagi
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Lage-Marques
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology / Q000627:therapeutic use; D000704:Analysis of Variance; D017610:Calcium Compounds / Q000494:pharmacology / Q000627:therapeutic use; D002448:Cell Adhesion; D002460:Cell Line; D003804:Dentin / Q000648:ultrastructure*; D003806:Dentin Permeability; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D010087:Oxides / Q000494:pharmacology / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000494:pharmacology / Q000627:therapeutic use*; D012391:Root Resorption / Q000473:pathology / Q000517:prevention & control*; D017640:Silicates / Q000494:pharmacology / Q000627:therapeutic use; D016369:Smear Layer; D013499:Surface Properties
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate