title: Erythrocyte fragility increases with level of glycosylated hemoglobin in type 2 diabetic patients.
abstract: BACKGROUND
  This study examines that erythrocyte was fragility-susceptible in diabetes.
  
  
  METHODS
  Forty-five outpatients with type 2 diabetes mellitus (aged 46 +/- 13 years) and 20 healthy individuals with no history of diabetes disorders (aged 43 +/- 7 years) were randomly selected in this study. All subjects were analysis for blood glucose, glycosylated hemoglobin and erythrocyte osmotic fragility tests.
  
  
  RESULTS
  The data for blood glucose (p<0.001), glycosylated hemoglobin (p<0.001), start hemolysis of erythrocyte (p<0.001), medium corpuscle hemolysis (p<0.001) and complete hemolysis (p=0.001) were significantly higher for diabetes than that of non-diabetes. The erythrocyte osmotic fragility in start hemolysis (r=0.479, p<0.001), medium corpuscle hemolysis (r=0.454, p<0.001) and complete hemolysis (r=0.277, p=0.025) were closely correlated with red blood cell glycosylated hemoglobin. Investigation of associations with red cell fragility-susceptible, diabetic patients had significantly increased risks of cell hemolysis on start hemolysis (ORs, 32.67; 95% CIs, 7.201-148.19), medium corpuscle hemolysis (ORs, 2.53; 95% CIs, 0.831-7.695) and complete hemolysis (ORs, 4.28; 95% CIs, 1.386-13.202) (all p<0.05 for linear trends) of erythrocyte to non-diabetes controls.
  
  
  CONCLUSIONS
  This study demonstrated that osmotic fragility of erythrocyte was greater in type 2 diabetic subjects compared to non-diabetic controls and red blood cell fragility was positively correlated with glycosylated hemoglobin. Hence, it is necessary to emphasize increasing investigations of pathogenic mechanisms exacerbated by red cell fragility to prevent complications of diabetes mellitus.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, College of Biomedical Science and Technology, Yuanpei University, No. 306 Road, Yuanpei, Hsinchu, Taiwan, Republic of China. jameskung@mail.ypu.edu.tw
authors.forename: Chien-Min
authors.initials: CM
authors.lastname: Kung
authors.forename: Zu-Lin
authors.initials: ZL
authors.lastname: Tseng
authors.forename: Hai-Lung
authors.initials: HL
authors.lastname: Wang
mesh_terms: D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D048909:Diabetes Complications / Q000097:blood / Q000517:prevention & control; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D006442:Glycated Hemoglobin / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D016014:Linear Models; D008875:Middle Aged; D009996:Osmotic Fragility
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin