title: Volumetric microvascular imaging of human retina using optical coherence tomography with a novel motion contrast technique.
abstract: Phase variance-based motion contrast imaging is demonstrated using a spectral domain optical coherence tomography system for the in vivo human retina. This contrast technique spatially identifies locations of motion within the retina primarily associated with vasculature. Histogram-based noise analysis of the motion contrast images was used to reduce the motion noise created by transverse eye motion. En face summation images created from the 3D motion contrast data are presented with segmentation of selected retinal layers to provide non-invasive vascular visualization comparable to currently used invasive angiographic imaging. This motion contrast technique has demonstrated the ability to visualize resolution-limited vasculature independent of vessel orientation and flow velocity.
authors.affiliation: California Institute of Technology, Pasadena, California 91125, USA. fingler@its.caltech.edu
authors.forename: Jeff
authors.initials: J
authors.lastname: Fingler
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Zawadzki
authors.forename: John S
authors.initials: JS
authors.lastname: Werner
authors.forename: Dan
authors.initials: D
authors.lastname: Schwartz
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
mesh_terms: D000792:Angiography / Q000379:methods; D003287:Contrast Media / Q000494:pharmacology; D003952:Diagnostic Imaging / Q000379:methods; D005133:Eye Movements; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D009038:Motion; D009389:Neovascularization, Pathologic; D055095:Optics and Photonics; D012160:Retina / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003287:Contrast Media