[Mechanism of endothelial Rho/Rho kinase in extravascular migration of fibrosarcoma cell].
OBJECTIVE
To study the mechanism of endothelial Rho/Rho kinase in extravascular migration of fibrosarcoma cell.


METHODS
We used an in vitro model of fibrosarcoma cell transmigration across a monolayer of human umbilical vein endothelial cell (HUVEC) cultured on collagen gel to observe extravascular migration of fibrosarcoma cells, and then calculated the electrical resistance of HUVEC monolayer and endothelial myosin light chain (MLC) phosphorylation in extravascular migration of fibrosarcoma cells.


RESULTS
Fibrosarcoma cells migrated through endothelial cells into collagen gel. The electrical resistance of a HUVEC monolayer reduced and endothelial MLC phosphorylation enhanced in the extravascular migration of fibrosarcoma cells. Endothelial Rho inhibitor (C3 transferase) and Rho kinase inhibitor (Y-27632) inhibited the extravascular migration of fibrosarcoma cells and inhibited the reduction of electrical resistance of a HUVEC monolayer and the enhancement of endothelial MLC phosphorylation in extravascular migration of fibrosarcoma cells.


CONCLUSIONS
Endothelial Rho/Rho kinase may regulate fibrosarcoma cell transendothelial migration through MLC phosphorylation.
Xin
Hua
H
Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
Gao
Zhong-Li
ZL
Han
Zhen-Guo
ZG
D000577:Amides / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005354:Fibrosarcoma / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D020741:rho GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D054460:rho-Associated Kinases / Q000378:metabolism / Q000502:physiology*
D000577:Amides; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases; D020741:rho GTP-Binding Proteins