title: Mycoplasma synoviae cell invasion: elucidation of the Mycoplasma pathogenesis in chicken.
abstract: Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was detected initially adhered to and subsequently inside the host cells. Between 24 and 48 h of infection, Ms was detected in the perinuclear region, and after 72 h of infection was confirmed by gentamicin invasion assay. High and low passage Ms strains showed no differences in adherence or invasion. The morphology and the actin filaments of the infected HEp-2 cells were preserved throughout the study period. The observed invasion by Ms is consistent with the biology of Mollicutes, and could explain the difficulties in recovering field isolates of the mycoplasma and in controlling the infection in birds even after long-term antibiotic treatment.
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Buim
authors.forename: Melissa
authors.initials: M
authors.lastname: Buzinhani
authors.forename: Maurício
authors.initials: M
authors.lastname: Yamaguti
authors.forename: Rosângela C
authors.initials: RC
authors.lastname: Oliveira
authors.forename: Elena
authors.initials: E
authors.lastname: Mettifogo
authors.forename: Priscila M
authors.initials: PM
authors.lastname: Ueno
authors.forename: Jorge
authors.initials: J
authors.lastname: Timenetsky
authors.forename: Gláucia M M
authors.initials: GM
authors.lastname: Santelli
authors.forename: Antonio J Piantino
authors.initials: AJ
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D002645:Chickens; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D005456:Fluorescent Dyes; D006801:Humans; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000502:physiology*; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes