Salicylic acid reduces napropamide toxicity by preventing its accumulation in rapeseed (Brassica napus L.).
Napropamide is a widely used herbicide for controlling weeds in crop production. However, extensive use of the herbicide has led to its accumulation in ecosystems, thus causing toxicity to crops and reducing crop production and quality. Salicylic acid (SA) plays multiple roles in regulating plant adaptive responses to biotic and environmental stresses. However, whether SA regulates plant response to herbicides (or pesticides) was unknown. In this study, we investigated the effect of SA on herbicide napropamide accumulation and biological processes in rapeseed (Brassica napus). Plants exposed to 8 mg kg(-1) napropamide showed growth stunt and oxidative damage. Treatment with 0.1 mM SA improved growth and reduced napropamide levels in plants. Treatment with SA also decreased the abundance of O (2) (-.) and H(2)O(2) as well as activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and increased activities of guaiacol peroxidase (POD) and glutathione-S-transferase (GST) in napropamide-exposed plants. Analysis of SOD, CAT, and POD activities using nondenaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. These results may help to understand how SA regulates plant response to organic contaminants and provide a basis to control herbicide/pesticide contamination in crop production.
Cui
Jing
J
Department of Applied Chemistry, Nanjing Agricultural University, China.
Zhang
Rui
R
Wu
Guo Lin
GL
Zhu
Hong Mei
HM
Yang
Hong
H
D001204:Ascorbate Oxidase / Q000378:metabolism; D029688:Brassica napus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism / Q000633:toxicity*; D009281:Naphthalenes / Q000378:metabolism / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D006540:Herbicides; D009281:Naphthalenes; D017382:Reactive Oxygen Species; D011392:Proline; C010909:devrinol; D001204:Ascorbate Oxidase; C121048:guaiacol peroxidase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D020156:Salicylic Acid