Protein nanocapsule weaved with enzymatically degradable polymeric network.
Target proteins can be functionally encapsulated using a cocoon-like polymeric nanocapsule formed by interfacial polymerization. The nanocapsule is cross-linked by peptides that can be proteolyzed by proteases upon which the protein cargo is released. The protease-mediated degradation process can be controlled in a spatiotemporal fashion through modification of the peptide cross-linker with photolabile moieties. We demonstrate the utility of this approach through the cytoplasmic delivery of the apoptosis inducing caspase-3 to cancer cells.
Gu
Zhen
Z
Department of Chemical and Biomolecular Engineering, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.
Yan
Ming
M
Hu
Biliang
B
Joo
Kye-Il
KI
Biswas
Anuradha
A
Huang
Yu
Y
Lu
Yunfeng
Y
Wang
Pin
P
Tang
Yi
Y
D020341:Absorbable Implants; D053148:Caspase 3 / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D053769:Nanocapsules; D011108:Polymers; D053148:Caspase 3