Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy.
We have extensively characterized the sarcomeric SHG signal as a function of animal species (rat versus xenopus), age (adult versus larval) and tissue preparation (fixed or fresh) and we found that the main feature of this signal is a single peak per mature sarcomere (about 85% of all sarcomeres). The remaining (15%) was found to be either double peak per mature sarcomere or mini sarcomeres (half of a sarcomere) using alpha-actinin immuno detection of the Z-band. The mini sarcomeres are often found in region of pitchfork-like SHG pattern. We suggest that double peak SHG pattern could indicate regions of sarcomeric proteolysis whereas pitchfork-like SHG pattern could reveal sarcomeric assembly.
Recher
Gaëlle
G
Equipe SCANING, UMR UR1-CNRS 6026, Rennes Cedex, France.
Rouède
Denis
D
Richard
Patrick
P
Simon
Antoine
A
Bellanger
Jean-Jacques
JJ
Tiaho
François
F
D000375:Aging / Q000473:pathology*; D000818:Animals; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000166:cytology*; D012518:Sarcomeres / Q000648:ultrastructure*; D013045:Species Specificity; D014982:Xenopus laevis