title: Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity.
abstract: Immune cells navigate through different environments where they experience different mechanical forces. Responses to external forces are determined by the mechanical properties of a cell and they depend to a large extent on the actin-rich cell cortex. We report here that Myo1G, a previously uncharacterised member of class I myosins, is expressed specifically in haematopoietic tissues and cells. It is associated with the plasma membrane. This association is dependent on a conserved PH-domain-like myosin I tail homology motif and the head domain. However, the head domain does not need to be a functional motor. Knockdown of Myo1G in Jurkat cells decreased cell elasticity significantly. We propose that Myo1G regulates cell elasticity by deformations of the actin network at the cell cortex.
authors.affiliation: Institute of General Zoology and Genetics, Westfalian Wilhelms-University Münster, Münster, Germany.
authors.forename: Balaji
authors.initials: B
authors.lastname: Olety
authors.forename: Mike
authors.initials: M
authors.lastname: Wälte
authors.forename: Ulrike
authors.initials: U
authors.lastname: Honnert
authors.forename: Hermann
authors.initials: H
authors.lastname: Schillers
authors.forename: Martin
authors.initials: M
authors.lastname: Bähler
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D004548:Elasticity / Q000502:physiology*; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology*; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009218:Myosins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034741:RNA, Small Interfering; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D000199:Actins; D034741:RNA, Small Interfering; D009218:Myosins