All-fiber CARS microscopy of live cells.
Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and an ultra-highly nonlinear fiber for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) non-linear optical microscopy of both tissue samples and live cells. Multimodal imaging was successfully performed with pixel dwell times as short as 4 micros at low laser powers (< 40 mW total).
Pegoraro
Adrian F
AF
Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Ridsdale
Andrew
A
Moffatt
Douglas J
DJ
Pezacki
John Paul
JP
Thomas
Brian K
BK
Fu
Libin
L
Dong
Liang
L
Fermann
Martin E
ME
Stolow
Albert
A
D000818:Animals; D045744:Cell Line, Tumor; D004867:Equipment Design; D004871:Erbium / Q000737:chemistry; D005336:Fiber Optic Technology; D006801:Humans; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D009991:Oscillometry / Q000379:methods; D011817:Rabbits; D013059:Spectrum Analysis, Raman / Q000379:methods*; D015018:Ytterbium / Q000737:chemistry
D004871:Erbium; D015018:Ytterbium