Neuronal differentiation of PC12 cells cultured on growth factor-loaded nanoparticles coated on PLGA microspheres.
The development of nanotechnology has penetrated the fields of biology and medicine, resulting in remarkable applications for tissue regeneration. In order to apply this technology to tissue engineering, we have developed nanoscaled 3D scaffolds consisting of growth factor-loaded heparin/poly(l-lysine) nanoparticles (NPs) attached to the surface of polymeric microspheres via polyionic complex methods. Growth factor-loaded NPs were simply produced as polyelectrolyte complexes with diameters of 100-200 nm. They were then coated onto positively charged poly(lacticco- glycolic acid) (PLGA) pretreated with polyethyleneimine to enable cell adhesion, proliferation, and stimulation of neurite outgrowth. Propidium iodide staining and beta-tubulin analysis revealed that neuronal PC12 cells proliferated extensively, expressed significant amounts of b-tubulin, and showed well-structured neurite outgrowth on polymeric microspheres by stimulation with growth factors. These results suggest that cellular adhesion and biological functionality on prepared PLGA microspheres enabled terminal differentiation of neuronal cells.
Park
Keun-Hong
KH
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Seoul 135-081, Korea.
Kim
Hyemin
H
Na
Kun
K
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D006493:Heparin / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D016716:PC12 Cells; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014404:Tubulin / Q000096:biosynthesis
D036341:Intercellular Signaling Peptides and Proteins; D014404:Tubulin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine; D006493:Heparin