title: Protein nanocapsule weaved with enzymatically degradable polymeric network.
abstract: Target proteins can be functionally encapsulated using a cocoon-like polymeric nanocapsule formed by interfacial polymerization. The nanocapsule is cross-linked by peptides that can be proteolyzed by proteases upon which the protein cargo is released. The protease-mediated degradation process can be controlled in a spatiotemporal fashion through modification of the peptide cross-linker with photolabile moieties. We demonstrate the utility of this approach through the cytoplasmic delivery of the apoptosis inducing caspase-3 to cancer cells.
authors.affiliation: Department of Chemical and Biomolecular Engineering, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gu
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Biliang
authors.initials: B
authors.lastname: Hu
authors.forename: Kye-Il
authors.initials: KI
authors.lastname: Joo
authors.forename: Anuradha
authors.initials: A
authors.lastname: Biswas
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lu
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D020341:Absorbable Implants; D053148:Caspase 3 / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D053769:Nanocapsules; D011108:Polymers; D053148:Caspase 3