Protection of chronic renal failure by a polysaccharide from Cordyceps sinensis.
A water-soluble polysaccharide (CPS-2), isolated from the cultured Cordyceps sinensis, was obtained by hot-water extraction, anion-exchange and gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, periodate oxidation, methylation analysis, FTIR and NMR spectroscopy. CPS-2 was found to be mostly of alpha-(1-->4)-D-glucose and alpha-(1-->3)-D-mannose, branched with alpha-(1-->4,6)-D-glucose every twelve residues on average. CPS-2 had a molecular weight of 4.39x10(4) Da. The protective effect of CPS-2 on the model of chronic renal failure was established by fulgerizing kidney. The changes in blood urea nitrogen and serum creatinine revealed that CPS-2 could significantly relieve renal failure caused by fulgerizing kidney.
Wang
Ying
Y
School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China.
Yin
Hongping
H
Lv
Xiaobo
X
Wang
Yufeng
Y
Gao
Hongyan
H
Wang
Min
M
D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D032661:Cordyceps / Q000737:chemistry*; D004195:Disease Models, Animal; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012076:Renal Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
D001688:Biological Products; D011134:Polysaccharides; D012076:Renal Agents