Effect of genetic adjuvants on immune respondance, growth and hormone levels in somatostatin DNA vaccination-induced Hu lambs.
The aim of current study was to evaluate the prospects of adjuvants against DNA vaccination (pES/2SS) encoding somatostatin (SS) and hepatitis B surface antigen fusion gene. A total of 60 female Hu lambs were divided into 6 groups and vaccinated in the context of various adjuvants (and controls): pE-CpG, Escherichia coli DH5alpha DNA, crude liposomes or GM-CSF in combination with the pES/2SS plasmid. Controls included pES/2SS only vaccinated and physiological saline groups. The antibody against SS level in the E. coli DH5alpha DNA group was significantly increased compared to that in the pES/2SS vaccine alone. Vaccination with pES/2SS/pE-CpG or pES/2SS/E. coli DH5alpha resulted in elevated weight gains that were 33.0 and 31.6% higher, respectively, than in saline group and pES/2SS only vaccinated controls. The concentrations of GH and IGF-I in the DNA vaccine groups were remarkably higher than those in the saline group, and those with positive antibody higher than negative antibody. These results suggested that different adjuvant/pES/2SS combinations can enhance the immune effect and had significant positive effects on growth.
Xue
Chun-Lin
CL
Key Lab of Education Ministry of China in Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Wuhan 430070, PR China.
Liang
Ai-Xin
AX
Mao
Da-Gan
DG
Han
Li
L
Liu
Xing-Bin
XB
Zhang
Jian
J
Yang
Li-Guo
LG
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D001835:Body Weight; D004926:Escherichia coli; D005260:Female; D013006:Growth Hormone / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D010957:Plasmids; D012756:Sheep / Q000254:growth & development* / Q000276:immunology; D013004:Somatostatin / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006514:Hepatitis B Surface Antigens; D019444:Vaccines, DNA; D013004:Somatostatin; D013006:Growth Hormone