title: Improved detection of microbial DNA after bead-beating before DNA isolation.
abstract: Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.
authors.affiliation: Department of Research & Development, Laboratory for Infectious Diseases Groningen, The Netherlands.
authors.forename: Richard
authors.initials: R
authors.lastname: de Boer
authors.forename: Remco
authors.initials: R
authors.lastname: Peters
authors.forename: Sonja
authors.initials: S
authors.lastname: Gierveld
authors.forename: Tim
authors.initials: T
authors.lastname: Schuurman
authors.forename: Mirjam
authors.initials: M
authors.lastname: Kooistra-Smid
authors.forename: Paul
authors.initials: P
authors.lastname: Savelkoul
mesh_terms: D001433:Bacteriolysis; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis*; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods
chemical_list: D004269:DNA, Bacterial