Adeno-associated virus harboring fusion gene NT4-ant-shepherdin induce cell death in human lung cancer cells.
To further enhance anticancer effect of Shepherdin and overcome limitation of peptide therapy, recombinant adeno-associated virus (rAAV) was constructed with following strategies: therapeutic peptide secretory expression and adeno-associated virus gene transfer system. MTT assay and flow cytometric analysis revealed that rAAV harboring fusion gene NT4-Ant-Shepherdin significantly suppressed A549 cell growth in a time-dependent manner and induced apoptosis. In the infected A549 cells, survivin expression level decreased strongly while Caspase-3/7 activities increased significantly. These results indicated that rAAV harboring fusion gene NT4-Ant-Shepherdin may be a novel strategy in lung cancer peptide therapy.
Xiaojiang
Tang
T
Department of Oncosurgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, China.
Jinsong
Zhou
Z
Jiansheng
Wang
W
Chengen
Pan
P
Guangxiao
Yang
Y
Quanying
Wang
W
D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D045744:Cell Line, Tumor; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008175:Lung Neoplasms / Q000628:therapy*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D000077022:Survivin; C503112:shepherdin; D020169:Caspases; C073922:neurotrophin 4