title: Antimicrobial compounds from the endophytic fungus Fusarium sp. Ppf4 isolated from the medicinal plant Paris polyphylla var. yunnanensis.
abstract: Two sterols and one fatty acid were obtained by bioassay-guided fractionation from the light petroleum extract of the fungus Fusarium sp. Ppf4 isolated from the rhizomes of Paris polyphylla var. yunnanensis Hand.-Mazz., a medicinal species distributed in the southwest of China. The compounds were elucidated as 5alpha, 8alpha-epidioxyergosta-6, 22-dien-3beta-ol (1), ergosta-8(9), 22-dien-3beta, 5alpha, 6beta, 7alpha-tetraol (2), and butanedioic acid (3), respectively, by means of physical and spectrometric analysis. Both fungal spore germination and micro-dilution-MTT assays were employed to evaluate their antimicrobial activity. Compound 1 was found to be the most bioactive, and compound 3 less active against the test pathogens. This is the first report of the antimicrobial activity of the compounds isolated from the endophytic Fusarium sp. Ppf4 associated with P. polyphylla var. yunnanensis. The results provide promising baseline information for the potential use of the compounds of this endophytic fungus as an antimicrobial agent to control plant and animal diseases.
authors.affiliation: College ofAgronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Huang
authors.forename: Jianglin
authors.initials: J
authors.lastname: Zhao
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
authors.forename: Mingan
authors.initials: M
authors.lastname: Wang
authors.forename: Jingguo
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D004396:Coloring Agents; D005670:Fusarium / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000382:microbiology*; D013171:Spores, Bacterial / Q000187:drug effects; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D013256:Steroids; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue