A proteomic approach for the identification of vascular markers of liver metastasis.
Vascular proteins expressed at liver metastasis sites could serve as prognostic markers or as targets for pharmacodelivery applications. We employed a proteomic approach to define such proteins in three syngeneic mouse models of liver metastasis. Vascular structures were biotinylated in vivo by a terminal perfusion technique, followed by mass spectrometric analysis of accessible biotinylated proteins. In this manner, we identified 12 proteins for which expression was selectively associated with liver metastasis, confirming this association by tissue immunofluorescence or in vivo localization with radiolabeled antibodies. In summary, our findings identify vascular proteins that may have prognostic or drug-targeting use in addressing liver metastases, a common issue in many advanced cancers.
Borgia
Beatrice
B
Department of Chemistry and Applied Biosciences, ETH Zürich, Zurich, Switzerland.
Roesli
Christoph
C
Fugmann
Tim
T
Schliemann
Christoph
C
Cesca
Marta
M
Neri
Dario
D
Giavazzi
Raffaella
R
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D001808:Blood Vessels / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D020869:Gene Expression Profiling; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000098:blood supply / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000473:pathology; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor