Selective removal of ovarian cancer cells from human ascites fluid using magnetic nanoparticles.
A majority of ovarian cancer metastases result from the shedding of malignant cells from the primary tumor into the abdominal cavity. Free-floating cancer cells in serous effusions of late-stage ovarian cancer patients may spread to internal organs, making effective treatment extremely difficult. Selective removal of ovarian cancer cells from serous fluids may abate metastasis and improve long-term prognoses. We have already shown that superparamagnetic nanoparticles conjugated to an ephrin-A1 mimetic peptide with a high affinity for the EphA2 receptor can be used to capture and remove cultured human ovarian cancer cells from the peritoneal of experimental mice. Here we demonstrate the potential clinical utility of the methodology by in vitro capture and isolation of cancer cells from the ascites fluid of ovarian cancer patients.


BACKGROUND
Ovarian cancer metastases usually are the result of shedding of malignant cells from the primary tumor into the abdominal cavity. In this paper, a novel nanotechnology-based method is demonstrated for the in vitro capture and isolation of cancer cells from the ascites fluid of ovarian cancer patients.
Scarberry
Kenneth E
KE
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Dickerson
Erin B
EB
Zhang
Z John
ZJ
Benigno
Benedict B
BB
McDonald
John F
JF
D000911:Antibodies, Monoclonal / Q000276:immunology; D001202:Ascitic Fluid / Q000473:pathology*; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008280:Magnetics / Q000379:methods*; D008856:Microscopy, Fluorescence; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles; D009362:Neoplasm Metastasis / Q000473:pathology / Q000517:prevention & control; D010051:Ovarian Neoplasms / Q000473:pathology*; D010455:Peptides / Q000378:metabolism
D000911:Antibodies, Monoclonal; D010455:Peptides; D016650:Fluorescein-5-isothiocyanate