Interaction of influenza virus NS1 protein with growth arrest-specific protein 8.
NS1 protein is the only non-structural protein encoded by the influenza A virus, and it contributes significantly to disease pathogenesis by modulating many virus and host cell processes. A two-hybrid screen for proteins that interact with NS1 from influenza A yielded growth arrest-specific protein 8. Gas8 associated with NS1 in vitro and in vivo. Deletion analysis revealed that the N-terminal 260 amino acids of Gas8 were able to interact with NS1, and neither the RNA-binding domain nor the effector domain of NS1 was sufficient for the NS1 interaction. We also found that actin, myosin, and drebrin interact with Gas8. NS1 and beta-actin proteins could be co-immunoprecipitated from extracts of transfected cells. Furthermore, actin and Gas8 co-localized at the plasma membrane. These results are discussed in relation to the possible functions of Gas8 protein and their relevance in influenza virus release.
Zhao
Lixia
L
Laboratory of protein engineering, Beijing Institute of Biotechnology, Beijing 100071, PR China. happylisa2008@yahoo.com.cn
Xu
Long
L
Zhou
Xiaowei
X
Zhu
Qingyu
Q
Yang
Zhixin
Z
Zhang
Chuanfu
C
Zhu
Xudong
X
Yu
Mengbin
M
Zhang
Yingying
Y
Zhao
Xinghui
X
Huang
Peitang
P
D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000378:metabolism*; D051379:Mice; D009218:Myosins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D011485:Protein Binding; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D003598:Cytoskeletal Proteins; C115742:GAS8 protein, human; C085820:INS1 protein, influenza virus; D009363:Neoplasm Proteins; D009479:Neuropeptides; D017361:Viral Nonstructural Proteins; C053386:drebrins; D009218:Myosins