Variable Field Proton-Electron Double-Resonance Imaging: Application to pH mapping of aqueous samples.
A new concept of Variable Field Proton-Electron Double-Resonance Imaging (VF PEDRI) is proposed. This allows for functional mapping using specifically designed paramagnetic probes (e.g. oxygen or pH mapping) with MRI high quality spatial resolution and short acquisition time. Studies performed at 200 G field MRI with phantoms show that a pH map of the sample can be extracted using only two PEDRI images acquired in 140 s at pre-selected EPR excitation fields providing pH resolution of 0.1 pH units and a spatial resolution of 1.25mm. Note that while concept of functional VF PEDRI was demonstrated using the pH probe, it can be applied for studies of other biologically relevant parameters of the medium such as redox state, concentrations of oxygen or glutathione using specifically designed EPR probes.
Khramtsov
Valery V
VV
Davis Heart and Lung Research Institute and the Division of Cardiovascular Medicine, The Ohio State University, College of Medicine, 420 West 12th Ave., Room 611B, Columbus, OH 43210, USA.
Caia
George L
GL
Shet
Keerthi
K
Kesselring
Eric
E
Petryakov
Sergey
S
Zweier
Jay L
JL
Samouilov
Alexandre
A
D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009589:Nitrogen Oxides / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D019047:Phantoms, Imaging; D011522:Protons; D026361:Reactive Nitrogen Species / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D009589:Nitrogen Oxides; D011522:Protons; D026361:Reactive Nitrogen Species; D014867:Water; D010100:Oxygen