Isolation of diapause-regulated genes from the flesh fly, Sarcophaga crassipalpis by suppressive subtractive hybridization.
Subtractive suppressive hybridization (SSH) was used to characterize the diapause transcriptome of the flesh fly Sarcophaga crassipalpis. Through these efforts, we isolated 97 unique clones which were used as probes in northern hybridization to assess their expression during diapause. Of these, 17 were confirmed to be diapause upregulated and 1 was diapause downregulated, while 12 were shown to be unaffected by diapause in this species. The diapause upregulated genes fall into several broad categories including heat shock proteins, heavy metal responsive genes, neuropeptides, structural genes, regulatory elements, and several genes of unknown function. In combination with other large-scale analyses of gene expression during diapause, this study assists in the characterization of the S. crassipalpis diapause transcriptome, and begins to identify common elements involved in diapause across diverse taxa.
Rinehart
Joseph P
JP
Ohio State University, Department of Entomology, 318 W. 12th Ave., Columbus, OH, USA. joseph.rinehart@ars.usda.gov
Robich
Rebecca M
RM
Denlinger
David L
DL
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics* / Q000502:physiology; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D017344:Genes, Insect / Q000235:genetics* / Q000502:physiology; D007301:Insect Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology
D007301:Insect Hormones; D004247:DNA