title: "Print-n-Shrink" technology for the rapid production of microfluidic chips and protein microarrays.
abstract: An innovative method for the production of microfluidic chips integrating protein spots is described. The technology, called "Print-n-Shrink", is based on the screen-printing of a microfluidic design (using a dielectric ink) onto Polyshrink polystyrene sheets. The initial print which has a minimum size of 15 microm (height) x 230 microm (width) is thermally treated (30 seconds, 163 degrees C) to shrink and generate features of 85 microm (height) x 100 microm (width). Concomitantly, proteins such as monoclonal antibodies or cellular adhesion proteins are spotted onto the Polyshrink sheets and shrunk together with the microfluidic design, creating a complete biochip integrating both complex microfluidic designs and protein spots for bioanalytical applications.
authors.affiliation: Laboratoire de Génie Enzymatique et Biomoléculaire, Institut de Chimie et Biochimie Moléculaire et Supramoléculaire, Université Lyon1 - CNRS 5246 ICBMS, Bat. CPE - 43 Bd du 11 Nov, 69622 Villeurbanne, France.
authors.forename: Kevin
authors.initials: K
authors.lastname: Sollier
authors.forename: Céline A
authors.initials: CA
authors.lastname: Mandon
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Heyries
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002417:Cattle; D017097:Electric Impedance; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay; D007281:Ink; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D011327:Printing; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D011137:Polystyrenes; D012710:Serum Albumin, Bovine