Spectrometric studies on the interaction of fluoroquinolones and bovine serum albumin.
The interaction between fluoroquinolones (FQs), ofloxacin and enrofloxacin, and bovine serum albumin (BSA) was investigated by fluorescence and UV-vis spectroscopy. It was demonstrated that the fluorescence quenching of BSA by FQ is a result of the formation of the FQ-BSA complex stabilized, in the main, by hydrogen bonds and van der Waals forces. The Stern-Volmer quenching constant, K(SV), and the corresponding thermodynamic parameters, DeltaH, DeltaS and DeltaG, were estimated. The distance, r, between the donor, BSA, and the acceptor, FQ, was estimated from fluorescence resonance energy transfer (FRET). The effect of FQ on the conformation of BSA was analyzed with the aid of UV-vis absorbance spectra and synchronous fluorescence spectroscopy. Spectral analysis showed that the two FQs affected the conformation of the BSA but in a different manner. Thus, with ofloxacin, the polarity around the tryptophan residues decreased and the hydrophobicity increased, while for enrofloxacin, the opposite effect was observed.
Ni
Yongnian
Y
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China. ynni@ncu.edu.cn
Su
Shaojing
S
Kokot
Serge
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002417:Cattle; D004735:Energy Transfer; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000378:metabolism*; D015242:Ofloxacin / Q000378:metabolism*; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D012710:Serum Albumin, Bovine; D000077422:Enrofloxacin; D015242:Ofloxacin