Adaptation of the highly productive T7 expression system to Streptomyces lividans.
Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.
Lussier
François-Xavier
FX
INRS-Institut Armand-Frappier, 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7. francois-xavier.lussier@iaf.inrs.ca
Denis
François
F
Shareck
François
F
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D002863:Chromogenic Compounds; D003001:Cloning, Molecular; D003062:Codon; D003227:Conjugation, Genetic; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005784:Gene Amplification; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012436:S-Adenosylmethionine / Q000235:genetics / Q000378:metabolism; D048170:Streptomyces lividans / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002863:Chromogenic Compounds; D003062:Codon; D003470:Culture Media; D004269:DNA, Bacterial; D012333:RNA, Messenger; D012436:S-Adenosylmethionine; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase