Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids.
Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM.
Kumar
Sandeep
S
School of Biotechnology, Faculty of science, Banaras Hindu University, Varanasi 221005, India.
Kayastha
Arvind M
AM
D001667:Binding, Competitive; D001888:Boric Acids / Q000737:chemistry / Q000494:pharmacology*; D001896:Boron Compounds / Q000737:chemistry / Q000494:pharmacology*; D001897:Boronic Acids / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008628:Mercury / Q000737:chemistry / Q000494:pharmacology; D019216:Metals, Heavy / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000201:enzymology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D012969:Sodium Fluoride / Q000737:chemistry / Q000494:pharmacology; D013025:Glycine max / Q000201:enzymology*; D014510:Urease / Q000037:antagonists & inhibitors*
D001888:Boric Acids; D001896:Boron Compounds; D001897:Boronic Acids; D004791:Enzyme Inhibitors; D019216:Metals, Heavy; D012834:Silver; C030433:n-butylboronic acid; C036647:4-bromophenylboric acid; D003300:Copper; D012969:Sodium Fluoride; D014510:Urease; D008628:Mercury; C010686:benzeneboronic acid; C032688:boric acid