title: Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.
abstract: F(2)-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F(2alpha), a urinary metabolite of 8-iso-prostaglandin F(2alpha.) Urine was purified by solid-phase extraction and analyzed by UPLC-MS/MS with negative-ion electrospray ionization. The method was robust with a mean inaccuracy of 9%, interday and intraday imprecision of 7.5% or lower, and a lower limit of quantification of 0.5 microg/L, equivalent to 0.04 pmol injected onto the column. An analysis time of 6 min was shorter than previously published methods and amenable to large studies.
authors.affiliation: Department of Pediatrics, Medical Genetics Division, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Haoyue
authors.initials: H
authors.lastname: Zhang
authors.forename: Dora
authors.initials: D
authors.lastname: Il'yasova
authors.forename: Judit
authors.initials: J
authors.lastname: Sztaray
authors.forename: Sarah P
authors.initials: SP
authors.lastname: Young
authors.forename: Frances
authors.initials: F
authors.lastname: Wang
authors.forename: David S
authors.initials: DS
authors.lastname: Millington
mesh_terms: D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028441:F2-Isoprostanes / Q000652:urine*; D006801:Humans; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C476546:2,3-dinor-8-iso-prostaglandin-F(2alpha); D015415:Biomarkers; D028441:F2-Isoprostanes