title: Ghrelin-liposome interactions: characterization of liposomal formulations of an acylated 28-amino acid peptide using CE.
abstract: Ghrelin is a pharmacologically interesting peptide hormone due to its effects on appetite and metabolism. The cationic, octanoylated 28 amino acid peptide has a short biological half-life; thus, prolonged release formulations are of interest. Acylated peptides have been suggested to bind to or be incorporated into liposomes. Formulations based on neutral dipalmitoylphosphatidylcholine (DPPC) liposomes and phosphatidylcholine:cholesterol (70:30 mol%) liposomes, and negatively charged dipalmitoylphosphatidylcholine:dipalmitoylphosphatidylserine (DPPC:DPPS) (70:30 mol%) liposomes (2 mM total lipid concentration) were characterized using ACE. Pre-equilibrium CZE and frontal analysis CE methods circumventing capillary wall adsorption of the peptide and the liposomes and suitable for characterizing ghrelin-liposome interactions were developed. The cationic peptide exhibited low affinity (<10% bound) for DPPC and phosphatidylcholine:cholesterol (70:30 mol%) liposomes whereas electrostatic interactions caused a higher affinity for DPPC:DPPS (70:30 mol%) liposomes. Studies on desacyl ghrelin instead of ghrelin demonstrated the significance of the n-octanoyl side chain as an affinity providing moiety towards DPPC:DPPS liposomes (48 and 73% bound peptide, respectively). CE experiments showed that the binding was characterized by rapid dissociation kinetics.
authors.affiliation: Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. joe@farma.ku.dk
authors.forename: Jesper
authors.initials: J
authors.lastname: Østergaard
authors.forename: Eva H
authors.initials: EH
authors.lastname: Moeller
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D054439:Ghrelin / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry
chemical_list: D054439:Ghrelin; D008081:Liposomes; D010718:Phosphatidylserines; D015060:1,2-Dipalmitoylphosphatidylcholine; C038694:dipalmitoylphosphatidylserine