title: Identifying sequences potentially related to resistance response of Piper tuberculatum to Fusarium solani f. sp. piperis by suppression subtractive hybridization.
abstract: Piper tuberculatum is an exotic Piper from the Amazon region that shows resistance to infection by Fusarium solani f. sp. piperis, causal agent of Fusarium disease in black pepper (Piper nigrum L.). In this work we aimed to study the interaction between P. tuberculatum and F. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to Fusarium disease resistance. Comparative sequence analysis confirmed that clones isolated here show a high identity with genes coding for proteins that have a known role in plant defense response mechanisms, such as peroxidase, hydroxyproline-rich glycoprotein and CBL-interacting protein kinase. The present study constitutes the first effort to understand the molecular basis of this plant-pathogen interaction, identifying genes which may be used in the future genetic improvement of black pepper.
authors.affiliation: Laboratório de Biologia Molecular, Universidade Federal do Pará, Belëm-PA, Brazil.
authors.forename: Soelange Bezerra
authors.initials: SB
authors.lastname: Nascimento
authors.forename: Júlio Cezar de Mattos
authors.initials: JC
authors.lastname: Cascardo
authors.forename: Ilmarina Campos de
authors.initials: IC
authors.lastname: Menezes
authors.forename: Maria de Lourdes Reis
authors.initials: Mde L
authors.lastname: Duarte
authors.forename: Sylvain
authors.initials: S
authors.lastname: Darnet
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Harada
authors.forename: Cláudia Regina Batista de
authors.initials: CR
authors.lastname: Souza
mesh_terms: D001483:Base Sequence; D005670:Fusarium; D006023:Glycoproteins / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009195:Peroxidase / Q000378:metabolism; D031701:Piper / Q000201:enzymology / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D011494:Protein Kinases / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D006023:Glycoproteins; D018626:Retroelements; D009195:Peroxidase; D011494:Protein Kinases