Anti-inflammatory effects of tanshinone IIA on radiation-induced microglia BV-2 cells inflammatory response.
OBJECTIVE
The aim of this study was to explore the inhibitory effects of Tanshinone II(A) on the production of proinflammation cytokines in radiation-stimulated microglia.


METHODS
Microglia cells were treated with 2, 4, 8, 16, and 32 Gy of irradiation or sham-irradiated in the presence or absence of 1.0 microg/mL of Tanshinone II(A). The effects of Tanshinone II(A) on radiation-induced proinflammatory cytokines were evaluated by real-time polymerase chain reaction; the expression level of nuclear factor (NF-kappabeta) p65 in cytoplasm and nucleus was measured by Western blot. Immunofluorescence staining and confocal microscopy analysis were applied to detect the expression of gamma-H2AX and p65 postirradiation.


RESULTS
Radiation-induced release of proinflammatory cytokines in BV-2 cells was detectable after irradiation. Tanshinone II(A) decreased the radiation-induced release of proinflammatory cytokines. Further, Western blotting showed that Tanshinone II(A) could attenuate the nuclear translocation of (NF-kappabeta) p65 submit postirradiation. Immunofluorescence staining showed gamma-H2AX foci formation with p65 translocation into the nucleus postirradiation.


CONCLUSIONS
Our data indicated that Tanshinone II(A) exerts anti-inflammatory properties by suppressing the transcription of proinflammatory cytokine genes that might be associated with the NF-kappabeta signaling pathway. It is postulated that irradiation causes immediate cellular reaction, and that double-strand breaks trigger the molecular response that leads to NF-kappabeta pathway activation.
Dong
Xiaorong
X
Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Dong
Jihua
J
Zhang
Ruiguang
R
Fan
Li
L
Liu
Li
L
Wu
Gang
G
D045784:Abietanes; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use* / Q000633:toxicity; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D017931:DNA Primers; D007249:Inflammation / Q000000981:diagnostic imaging / Q000188:drug therapy*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D010616:Phenanthrenes / Q000627:therapeutic use* / Q000633:toxicity; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D011877:Radionuclide Imaging; D011878:Radiotherapy / Q000009:adverse effects
D045784:Abietanes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D010616:Phenanthrenes; C021751:tanshinone; D012313:RNA