[Association of SNPs in N-formylpeptide receptor gene with susceptibility of aggressive periodontitis].
OBJECTIVE
To explore the association of the single nucleotide polymorphisms (SNPs) in N-formylpeptide receptor (FPR) gene with the susceptibility of aggressive periodontitis (AgP).


METHODS
A total of 94 AgP patients and 73 healthy controls were entered into the study. Peripheral blood sample was obtained from each subject by venepuncture. Genomic DNA was isolated from each sample. The target fragment of FPR gene was amplified by PCR. The SNPs in FPR gene were detected by denature high performance liquid chromatography (DHPLC) combined with DNA sequencing.


RESULTS
There were two non-synonymous SNPs in the 370 bp FPR gene fragment: 289C/A and 301G/C. The 289C/A was a novel SNP. No variation in nucleotides 329 and 378 was detected. There were no statistically significant differences in distributions of the genotypes and alleles for FPR289 and FPR301 between AgP patients and healthy controls. Using multivariate logistic regression (adjusted for age and gender), it was showed that the adjusted ORs of AgP for the C(+) genotype and allele C of FPR301 combined with smoking were 5.74 and 5.20 respectively.


CONCLUSIONS
The presence of the C(+) genotype/allele C of FPR301 together with smoking conferred a higher risk for AgP. The result suggests that the SNPs in FPR gene may not be associated with the susceptibility of AgP in Chinese.
Zhu
Xiao-ling
XL
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Meng
Huan-xin
HX
Zhang
Li
L
Xu
Li
L
Chen
Zhi-bin
ZB
Shi
Dong
D
Feng
Xiang-hui
XH
D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D044042:Receptors, Formyl Peptide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
D044042:Receptors, Formyl Peptide