ENU mutagenesis to generate genetically modified rat models.
The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
van Boxtel
Ruben
R
Hubrect Institute, Niob Knaw, Utrecht, The Netherlands.
Gould
Michael N
MN
Cuppen
Edwin
E
Smits
Bart M G
BM
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005038:Ethylnitrosourea / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008297:Male; D016296:Mutagenesis; D009154:Mutation; D051381:Rats / Q000235:genetics*
D004247:DNA; D005038:Ethylnitrosourea