title: A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber.
abstract: During inflammation neutrophils rapidly migrate to the site of tissue damage or infection by following complex gradients of bacterial peptides and host-derived chemokines. The efficiency and speed of neutrophil migration is critically dependent upon the ability of neutrophils to sense new gradients and utilize only those that provide the most direct path to the damaged or infected site. Receptor desensitization plays an important role in migration efficiency and is most commonly studied using bath application of chemotactic factor solutions instead of presenting cells with gradients analogous to those they would experience in vivo. Here we describe a new method for examining gradient-induced neutrophil desensitization using a previously-developed open-chamber microfluidic gradient generator.
authors.affiliation: Stem Cell and Regenerative Medicine Center, Madison, WI, USA. tkeenan@wisc.edu
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Keenan
authors.forename: Charles W
authors.initials: CW
authors.lastname: Frevert
authors.forename: Aileen
authors.initials: A
authors.lastname: Wu
authors.forename: Venus
authors.initials: V
authors.lastname: Wong
authors.forename: Albert
authors.initials: A
authors.lastname: Folch
mesh_terms: D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000737:chemistry*; D002633:Chemotaxis / Q000502:physiology*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D020556:Neutrophil Infiltration / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology
chemical_list: D002630:Chemotactic Factors; D005456:Fluorescent Dyes