[Expression of co-stimulatory molecule CD86 and its inducible co-stimulator in Crohn disease and their pathologic significance].
OBJECTIVE
To investigate the expression of co-stimulatory molecule CD86 and inducible co-stimulator(ICOS) in the intestinal mucosa of Crohn disease (CD) and to exlpore its pathologic significance.


METHODS
Expression of co-stimulator CD86 and ICOS was examined by immunohistochemistry on paraffin embedded tissue from patients with CD (30 cases) and normal controls (20 cases). The subsets of lamina propria mononuclear cells (LPMC) were also analysed via immunostaining for CD4, CD8 and CD20.


RESULTS
Increased amount of CD86 or ICOS positive LPMC was observed in the lesional area of CD when compared with the essentially normal area of CD and normal controls (q=9.23,P<0.01 and q=5.46,P<0.01). In addition, the expression of CD86 or ICOS was higher in intestinal epithelium of CD than that in normal controls(H=24.93,P<0.01 and H=4.66,P<0.01), whereas no significant difference was seen between the diseased and the essentially normal area of CD. The amount of CD4 or CD8 positive lymphocytes in lamina propria, epithelium and small vascular walls was also significantly increased in CD than that in normal controls (P<0.05 or P<0.01).


CONCLUSIONS
Increased amount of CD86 or ICOS positive LPMC and enterocytes in CD suggests that co-stimulatory molecules may play a role in the pathogenesis of CD. The enterocytes may act as non-specific antigen presenting cells in the process of cellular immunity activation in CD.
Chen
A-jing
AJ
Department of Pathology, Peking University School of Basic Medical Science, Beijing 100191, China.
Li
Fang
F
DU
Juan
J
Zhang
Yan
Y
Gong
En-cong
EC
Shi
Xue-ying
XY
D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051940:B7-2 Antigen / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D003424:Crohn Disease / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein; D007413:Intestinal Mucosa / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000945:Antigens, Differentiation, T-Lymphocyte; D051940:B7-2 Antigen; C497964:CD86 protein, human; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein