title: Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family.
abstract: We report the characterization of a new member of the low-density lipoprotein receptor (LDLR) gene family designated LRP10. Human LRP10 cDNA encodes a 1905 amino acid type I membrane protein consisting of five functional domains characteristic of the LDLR gene family. CHO-ldlA7 cells transfected with human LRP10 cDNA bound LDLR-associated protein, but not beta-VLDL and HDL. Human LRP10 transcripts were primarily found in the brain, muscle and heart. In situ hybridization of the rat brain showed that the transcripts were intensely present in the cerebral cortex, hippocampus, choroid plexus, ependyma and granular layer. In the developing rat brain, transcript levels gradually increased from postnatal day 1 to 20. Immunofluorescence analysis indicated that LRP10 was observed in the ventricular zone of the embryonic day 14.5 mouse cerebral cortex. The present studies suggest that LRP10 may play a significant role in the brain physiology other than lipoprotein metabolism.
authors.affiliation: Department of Molecular and Biochemical Nutrition, Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Jeong
authors.forename: Kayoko
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Someya
authors.forename: Akemi
authors.initials: A
authors.lastname: Hosoda
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshimi
authors.forename: Chikako
authors.initials: C
authors.lastname: Yokoyama
authors.forename: Sumiko
authors.initials: S
authors.lastname: Kiryu-Seo
authors.forename: Man-Jong
authors.initials: MJ
authors.lastname: Kang
authors.forename: Taro
authors.initials: T
authors.lastname: Tchibana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyama
authors.forename: Tomoe
authors.initials: T
authors.lastname: Fukumura
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saeki
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
chemical_list: D026502:LDL-Receptor Related Proteins; C510813:LRP10 protein, human