title: Characterization of the insoluble proteome of Lactococcus lactis by SDS-PAGE LC-MS/MS leads to the identification of new markers of adaptation of the bacteria to the mouse digestive tract.
abstract: We characterized the insoluble proteome of Lactococcus lactis using 1D electrophoresis-LC-MS/MS and identified 313 proteins with at least two different peptides. The identified proteins include 89 proteins having a predicted signal peptide and 25 predicted to be membrane-located. In addition, 67 proteins had alkaline isoelectric point values. Using spectra and peptide counts, we compared protein abundances in two different conditions: growth in rich medium, and after transit in the mouse digestive tract. We identified the large mechanosensitive channel and a putative cation transporter as membrane markers of bacterial adaptation to the digestive tract.
authors.affiliation: INRA, PAPPSO (Plate-Forme d'Analyse Protéomique de Paris Sud-Ouest), Jouy en Josas, France.
authors.forename: Jasna
authors.initials: J
authors.lastname: Beganović
authors.forename: Alain
authors.initials: A
authors.lastname: Guillot
authors.forename: Maarten
authors.initials: M
authors.lastname: van de Guchte
authors.forename: Anne
authors.initials: A
authors.lastname: Jouan
authors.forename: Christophe
authors.initials: C
authors.lastname: Gitton
authors.forename: Valentin
authors.initials: V
authors.lastname: Loux
authors.forename: Karine
authors.initials: K
authors.lastname: Roy
authors.forename: Sylvie
authors.initials: S
authors.lastname: Huet
authors.forename: Hervé
authors.initials: H
authors.lastname: Monod
authors.forename: Véronique
authors.initials: V
authors.lastname: Monnet
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D013294:Lactococcus lactis / Q000378:metabolism*; D051379:Mice; D020543:Proteome; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome