title: Classical RS1 and environmental RS1 elements in Vibrio cholerae O1 El Tor strains harbouring a tandem repeat of CTX prophage: revisiting Mozambique in 2005.
abstract: Currently, Vibrio cholerae O1 serogroup biotype El Tor strains producing classical type cholera toxin (altered strains or El Tor variants) are prevalent in Asia and in Mozambique. Mozambican strains collected in 2004 contained a tandem repeat of CTX prophage on the small chromosome and each CTX prophage harboured the classical rstR and classical ctxB. We found that the majority of the strains collected in 2005 in Mozambique contained extra elements on the large chromosome in addition to the tandem repeat of CTX prophage on the small chromosome. New type RS1 elements RS1(cla) and RS1(env), and a CTX(env) with rstR(env) and the classical ctxB were identified on the large chromosome of the Mozambican isolates collected in 2005.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Je Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Hye Ri
authors.initials: HR
authors.lastname: Lee
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Yoon-Seong
authors.initials: YS
authors.lastname: Jeon
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Lorenz
authors.initials: L
authors.lastname: von Seidlein
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Deen
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh.
authors.forename: M
authors.initials: M
authors.lastname: Ansaruzzaman
authors.affiliation: Ministry of Health, Maputo, Mozambique.
authors.forename: G Marcelino E S
authors.initials: GMES
authors.lastname: Lucas
authors.affiliation: Ministry of Health, Maputo, Mozambique.
authors.forename: Avertino
authors.initials: A
authors.lastname: Barreto
authors.affiliation: Ministry of Health, Maputo, Mozambique.
authors.forename: Francisco F
authors.initials: FF
authors.lastname: Songane
authors.affiliation: Centro de Higiene Ambiental e Exames Medicos, Beira, Mozambique.
authors.forename: Catarina
authors.initials: C
authors.lastname: Mondlane
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Cecil
authors.initials: C
authors.lastname: Czerkinsky
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: John D
authors.initials: JD
authors.lastname: Clemens
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009073:Mozambique / Q000453:epidemiology; D039002:Prophages / Q000235:genetics*; D017422:Sequence Analysis, DNA; D026801:Synteny; D020080:Tandem Repeat Sequences; D041241:Vibrio cholerae O1 / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D002772:Cholera Toxin