Combined effects of probiotic fermentation and high-pressure extraction on the antioxidant, antimicrobial, and antimutagenic activities of deodeok (Codonopsis lanceolata).
This study was designed to evaluate the combined effects of probiotic fermentation and high-pressure extraction (HPE) on the functional properties of Codonopsis lanceolata. The ground C. lanceolata samples were anaerobically fermented with Lactobacillus acidophilus ADH, Bifidobacterium longum B6, Lactobacillus rhamnosus GG, or Lactobacillus paracasei at 37 degrees C for 10 days and subjected to 500 MPa at 50 degrees C for 30 min. The extraction yields of C. lanceolata samples were noticeably increased to 29-32% by HPE. The B. longum-fermented C. lanceolata samples extracted by high pressure (BLF-HPE) exhibited the highest antimicrobial activity (MIC < 14 mg/mL) against Listeria monocytogenes, Staphylococcus aureus, Shigella boydii, and Salmonella typhimurium. The nonfermented C. lanceolata samples extracted with high pressure (NF-HPE) had the highest total phenolic content (13.3 mg of GAE/g). The lowest effective concentrations (EC(50) and EC(0.5)) were 4.55 and 1.76 mg/mL, respectively, for NF-HPE extracts, indicating its highest antioxidant activity. The BLF-HPE and L. rhamnosus-fermented C. lanceolata samples extracted by high pressure (LRF-HPE) exhibited the highest antimutagenic activities in S. typhimurium TA 100, which were 82 and 83% inhibition, respectively. The use of probiotic fermentation and HPE can produce more biologically active compounds in C. lanceolata than the conventional solvent extraction method. The results provide pharmaceutically useful information for improving biological properties and an approach to drug discovery.
He
Xinlong
X
Division of Biomaterials Engineering, Kangwon National University, Medical and Bio-Material Research Center, Chuncheon, Gangwon 200-701, Republic of Korea. juheeahn@kangwon.ac.kr
Kim
Seung-Seop
SS
Park
Sung-Jin
SJ
Seong
Dong-Ho
DH
Yoon
Won-Byong
WB
Lee
Hyeon-Yong
HY
Park
Dong-Sik
DS
Ahn
Juhee
J
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001644:Bifidobacterium / Q000378:metabolism*; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D029745:Codonopsis / Q000737:chemistry* / Q000382:microbiology; D005285:Fermentation; D007778:Lactobacillus / Q000378:metabolism*; D011312:Pressure; D019936:Probiotics / Q000378:metabolism
D000890:Anti-Infective Agents; D016587:Antimutagenic Agents; D000975:Antioxidants