Atypical functional properties of GluK3-containing kainate receptors.
The properties of synaptic receptors determine their mode of action at presynaptic and postsynaptic loci. Here, we investigated the atypical biophysical properties of GluK3-containing kainate receptors, which contribute to presynaptic facilitation at hippocampal mossy fiber synapses. We show, using fast glutamate applications on outside-out patches and kinetic modeling, that the low sensitivity of GluK3 receptors for glutamate is attributable to fast desensitization of partially bound receptors. Consequently, these receptors can only be activated by fast transients of high glutamate concentration. In addition, GluK3 receptors are very sensitive to voltage-dependent block by intracellular spermine that precludes activation of substantial currents at potentials positive to -50 mV. Two specific residues within the channel pore define this high-affinity site. Finally, GluK3 are calcium permeable in the same way as unedited GluK2 receptors. These receptors present unique properties among AMPA/kainate receptors that could reflect a specialized presynaptic function.
Perrais
David
D
Laboratoire Physiologie Cellulaire de la Synapse, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5091, Bordeaux Neuroscience Institute, University of Bordeaux, 33076 Bordeaux, France. david.perrais@u-bordeaux2.fr
Coussen
Françoise
F
Mulle
Christophe
C
D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism; D006624:Hippocampus / Q000737:chemistry / Q000502:physiology; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008564:Membrane Potentials / Q000502:physiology; D008959:Models, Neurological; D008969:Molecular Sequence Data; D009154:Mutation; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D018408:Patch-Clamp Techniques; D018092:Kainic Acid Receptors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013096:Spermine / Q000737:chemistry / Q000378:metabolism; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor
D002118:Calcium; D018698:Glutamic Acid; D018092:Kainic Acid Receptors; D013096:Spermine; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor