title: Antioxidant activity and haemolysis prevention efficiency of polyaniline nanofibers.
abstract: Polyaniline (PAni) nanofibers have been synthesized by interfacial polymerization using hydrochloric acid (HCl) and camphor sulfonic acid (CSA) as dopants. The powder x-ray diffraction pattern of bulk polyaniline reveals ES I structure and has been indexed in a pseudo-orthorhombic lattice. The broadening of (110) reflection in the nanofiber samples has been analysed in terms of domain length and strain using a convolution method employing a Voigt function. The increase in d spacing for the (110) reflection in HCl-doped PAni nanofibers have been assigned to the change in structural conformation due to the increase in the tilt angle of the polymer chain, which is also evident from microRaman spectra. UV-vis spectra of the PAni nanofibers exhibit a remarkable blueshift in the absorption bands attributed to pi-pi* and pi-polaron band transitions indicating a reduction in particle size, which is also observed in TEM micrographs. The antioxidant activity of the polyaniline nanofiber samples has been investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay by employing UV-visible spectroscopy. It has also been observed that polyaniline nanofibers are able to protect the haemolysis of red blood cells (RBCs) from cytotoxic agents, namely H(2)O(2). The observed enhancement in the antioxidant and haemolysis prevention activity of the PAni nanofibers as compared to bulk has been attributed to the reduction in particle size and changes in structural conformation, as evident from TEM, XRD and microRaman spectroscopy.
authors.affiliation: Department of Physics, Tezpur University, Tezpur, Assam, India.
authors.forename: Somik
authors.initials: S
authors.lastname: Banerjee
authors.forename: Jyoti P
authors.initials: JP
authors.lastname: Saikia
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: B K
authors.initials: BK
authors.lastname: Konwar
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds; D006461:Hemolysis; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010851:Picrates; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D014961:X-Ray Diffraction
chemical_list: D000814:Aniline Compounds; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; C416807:polyaniline; C004931:1,1-diphenyl-2-picrylhydrazyl