High stability of original predominant pospiviroid genotypes upon mechanical inoculation from ornamentals to potato and tomato.
Eleven pospiviroid isolates from ornamental plants and one from pepper were successfully transmitted to potato and tomato by mechanical inoculation. All isolates with characteristic predominant genotypes were inoculated to a series of potato and tomato plants and propagated for up to four passages. In total, 385 nucleotide sequences were determined, in which 17 new predominant genotypes were identified with minimal differences to the original predominant genotype. In addition, in the original ornamental hosts, only one of eleven predominant pospiviroid genotypes had changed during the experiments over a period of 2 years. These results confirm the high stability of predominant pospiviroid genotypes.
Verhoeven
J Th J
JT
Plant Protection Service, 6700, HC, Wageningen, The Netherlands. j.th.j.verhoeven@minlnv.nl
Roenhorst
J W
JW
D042822:Genomic Instability; D005838:Genotype; D010935:Plant Diseases / Q000821:virology*; D010944:Plants / Q000821:virology*; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D014772:Viroids / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification