Monosodium glutamate induces apoptosis in naive and memory human B cells.
The aim of this study was to establish the existence of mGluR7 in normal B lymphocytes and analyse the effect of monosodium glutamate (MSG) on B cell apoptosis in vitro. B cells were purified by magnetic cell sorting using anti-CD19-coupled magnetic beads. Cells (10(6)/ml) were cultured with increasing MSG concentrations (1-100 mM). Detection of apoptosis by flow cytometry was performed using the Annexin V-FITC/Propidium iodide (PI) apoptosis detection kit. Naïve and memory B cell population were identified by CD27 staining. Expression of GluRs was determined using PCR. Exposure to increasing MSG concentrations displayed dose dependent effect on B cell viability altogether, ranging from 35% with 100 mM up to 80% with 1 mM MSG. Moreover, the number of late apoptotic cells as well as necrotic cells was dose dependant. Both CD27- as well as CD27+ B cells were affected by MSG. Basal expression of GluRs7 was detected in unstimulated B cells. Glutamate induced apoptosis can be seen in memory as well as naive B cell population and is probably mediated through mGluR7, whose expression in B cells we also confirmed. Our study suggests a new possible mechanism of crosstalk between the nervous and the immune system through glutamate as a potential key mediator (Fig. 4, Ref. 27). Full Text (Free, PDF) www.bmj.sk.
Jovic
Z
Z
Institute of Pharmacology and Toxicology, Medical Faculty Nis, University of Nis, Serbia.
Veselinovic
M
M
Vasic
K
K
Stankovic-Djordjevic
D
D
Cekic
S
S
Milovanovic
M
M
Sarac
M
M
D018941:Antigens, CD19 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005503:Food Additives / Q000494:pharmacology*; D006801:Humans; D007156:Immunologic Memory; D018092:Kainic Acid Receptors / Q000378:metabolism; D012970:Sodium Glutamate / Q000494:pharmacology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis; D000097808:GluK3 Kainate Receptor
D018941:Antigens, CD19; D005503:Food Additives; D018092:Kainic Acid Receptors; D012970:Sodium Glutamate; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D000097808:GluK3 Kainate Receptor