title: Identification of acrolein-conjugated protein in plasma of patients with brain infarction.
abstract: It is known that the level of protein-conjugated acrolein in plasma is a good marker of chronic renal failure and brain infarction. Thus, studies were carried out to determine which kinds of plasma proteins are conjugated with acrolein. It was found that acrolein was mainly conjugated with albumin. Tandem mass spectrometry analysis demonstrated that Lys-557 and Lys-560, located at the surface of domain III of albumin, were the major sites conjugated with acrolein. This is the first report to identify the amino acid residues in a protein modified by acrolein in vivo. It was found that conjugation of acrolein with albumin contributed to a decrease in the toxicity of acrolein.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kyohei
authors.initials: K
authors.lastname: Higashi
authors.forename: Lihua
authors.initials: L
authors.lastname: Jin
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Machi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Akiko
authors.initials: A
authors.lastname: Masuda
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Akiko
authors.initials: A
authors.lastname: Suganami
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nishimura
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toida
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tomitori
authors.forename: Keiko
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Kazuei
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D000171:Acrolein / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D020520:Brain Infarction / Q000097:blood*; D006801:Humans; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D012709:Serum Albumin; D000171:Acrolein; D008239:Lysine