title: Human papillomavirus types 16 and 18 mRNA levels and not DNA levels may be associated with advancing stages of cervical cancer.
abstract: OBJECTIVE
  Human papillomavirus (HPV) contributes to the development of cervical cancer. We hypothesize that HPV DNA and messenger RNA (mRNA) levels may be associated with increasing stages of cervical cancer.
  
  
  METHODS
  In this study, we measured DNA and mRNA viral loads of the most common high-risk HPV-16 and HPV-18 in cervical biopsy tissue of women with cervical neoplasia using real-time polymerase chain reaction.
  
  
  RESULTS
  Median HPV-16 and HPV-18 DNA viral loads were 58,342 copies and 71,367 per 5000 cells, respectively. We found that HPV-16 and HPV-18 DNA levels did not correlate with advancing tumor stage (P = 0.977 and P = 0.263). Messenger RNA transcripts were detected in 81 (86%) of HPV-16 DNA-positive women and in 16 (84.2%) of HPV-18-positive women. Median HPV-16 and HPV-18 transcript copy numbers were 5964 and 6158, respectively. In women with squamous cell carcinoma, HPV-16 mRNA loads showed an increasing but not statistically significant trend with advancing disease stage (rho = 0.231, P = 0.058).
  
  
  CONCLUSIONS
  We conclude that HPV mRNA levels and not DNA levels may be associated with advancing stages of cervical cancer.
authors.affiliation: Department of Clinical Virology, Christian Medical College, Vellore, India.
authors.forename: Manu
authors.initials: M
authors.lastname: Gnanamony
authors.forename: Abraham
authors.initials: A
authors.lastname: Peedicayil
authors.forename: John
authors.initials: J
authors.lastname: Subhashini
authors.forename: Thomas Samuel
authors.initials: TS
authors.lastname: Ram
authors.forename: Solomon
authors.initials: S
authors.lastname: Christopher
authors.forename: Patti
authors.initials: P
authors.lastname: Gravitt
authors.forename: Priya
authors.initials: P
authors.lastname: Abraham
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000821:virology; D019562:Viral Load; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000235:genetics / Q000821:virology
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; C059731:oncogene protein E7, Human papillomavirus type 16