Prevalence and molecular characterization of fluoroquinolone-resistant Escherichia coli isolated from diarrheic cattle in Korea.
A total of 176 E. coli isolates were retrieved from 203 diarrheic fecal samples collected from Korean cattle on 117 different farms. The most frequently observed resistance in E. coli isolates was to tetracycline (88.6%), followed by streptomycin (80.7%) and ampicillin (64.8%). Resistance to cefazolin, cefoperazone, cefepime and amikacin was very low. Of the 176 E. coli strains, forty (22.7%) isolates from 30 farms showed resistance to fluoroquinolones (FQ). All the FQ-resistant strains possessed double mutations at codons 83 and 87 in the gyrA gene, and a single mutation mostly at codon 80 in the parC gene, except in one isolate. The pulsed-field gel electrophoresis profiles of the FQ-resistant E. coli isolates were heterogeneous, but two or three isolates that showed an identical pattern originated from the same or different farms. This study demonstrates that FQ resistance is frequently observed in E. coli from diarrheic cattle and that mutations in the quinolone resistance-determining region are the same as those seen in E. coli originating from other animal species and humans. The FQ resistance in diarrheic cattle might have been mostly acquired independently, although the possibility of transmission of FQ-resistant E. coli within a farm or between farms is plausible.
Lim
Suk-Kyung
SK
National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, 480 Anyang, Kyonggido, Republic of Korea.
Lim
Keum-Gi
KG
Lee
Hee-Soo
HS
Jung
Suk-Chan
SC
Kang
Mun-Il
MI
Nam
Hyang-Mi
HM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D007723:Korea / Q000453:epidemiology
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase