title: Effect of cefuroxime and moxifloxacin on Staphylococcus epidermidis adherence to intraocular lenses.
abstract: OBJECTIVE
  To investigate and compare the effect of cefuroxime and moxifloxacin on adhesion of Staphylococcus epidermidis to intraocular lenses (IOLs).
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  The 3-pieced hydrophobic acrylic lenses were contaminated with S. epidermidis (American Type Culture Collection 35983) solutions containing 108 colony-forming units. IOLs were inoculated into test tubes containing tryptic soy broth after being held in antibiotic solutions for 15 minutes. Sonication and vortex procedures were performed in order to remove all the remaining bacteria. From each tube 10 microL and 100 microL was taken and inoculated into sheep blood agar. The colonies were counted overnight. The statistical analyses were made using one-way ANOVA, Turkey Honestly Significant Differences test (HSD) and independent t tests, and a p value less than 0.05 was considered statistically significant.
  
  
  RESULTS
  Overall, the mean numbers of colony-forming units on the lenses that were held in control, cefuroxime, moxifloxacin 0.5 mg/0.1 mL and moxifloxacin 0.1 mg/0.1 mL solutions were 1398 (SE 10.01 x 10(3)), 29.9 (SE 1.16 x 10(3)), 0.23 (SD 0.04 x 10(3)), and 0.41 (SD 0.05 x 10(3)), respectively. The evaluation using one-way ANOVA and Turkey HSD tests revealed significant statistical differences among the groups (p = 0.000). The evaluation using independent t tests revealed significant statistical differences between the 2 moxifloxacin groups (p < 0.05).
  
  
  CONCLUSIONS
  Our results suggest that moxifloxacin and cefuroxime significantly inhibit bacterial adherence to IOLs. The effect of moxifloxacin on inhibition of bacterial adherence was significantly greater than that of cefuroxime. For this reason moxifloxacin might be considered as a better prophylactic agent.
authors.affiliation: Ophthalmology Department, Kocaeli University, School of Medicine, Kocaeli, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Karadağ
authors.forename: Berna
authors.initials: B
authors.lastname: Ozkan
authors.forename: V Levent
authors.initials: VL
authors.lastname: Karabaş
authors.forename: Ozgül
authors.initials: O
authors.lastname: Alintaş
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yumuk
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Cağlar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001372:Aza Compounds / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002444:Cefuroxime / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024841:Fluoroquinolones; D007910:Lenses, Intraocular / Q000382:microbiology*; D008689:Methacrylates; D000077266:Moxifloxacin; D011108:Polymers; D011804:Quinolines / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D008689:Methacrylates; D011108:Polymers; D011804:Quinolines; C104020:poly(methylmethacrylate-co-ethylhexylmethacrylate); D002444:Cefuroxime; D000077266:Moxifloxacin