Blood cytokines in rats with various behavioral characteristics during emotional stress and treatment with interleukin-1beta.
We studied the effect of acute emotional stress and exogenous IL-1beta (5 microg/kg intraperitoneally) on the cytokine profile of blood serum in Wistar rats with various behavioral characteristics in the open-field test. Blood level of proinflammatory cytokine IL-1beta decreased in behaviorally passive rats, but increased in active animals after simultaneous immobilization and electrocutaneous stimulation. These changes reflect the opposite immune responses to a similar stress exposure in rats with different emotional reactivity. Poststress variations in the concentration of circulating IL-1beta differed in rats receiving exogenous IL-1beta. Blood cytokine concentration decreased in behaviorally active rats, but remained unchanged in passive animals that were exposed to immobilization and electrocutaneous stimulation after pretreatment with IL-1beta. Emotional stress and injection of IL-1beta had no effect on blood level of an anti-inflammatory cytokine IL-4 in rats. Our results indicate that rats with various behavioral parameters are characterized by significant differences in the cytokine profile of blood serum under conditions of emotional stress and treatment with IL-1beta. These data illustrate the specific functional features of immune mechanisms, which provide an individual resistance of rats to the same stress exposure.
Pertsov
S S
SS
P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. s.pertsov@mail.ru
Koplik
E V
EV
Stepanyuk
V L
VL
Simbirtsev
A S
AS
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use*; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D051381:Rats; D013315:Stress, Psychological / Q000097:blood* / Q000188:drug therapy* / Q000473:pathology
D016207:Cytokines; D053583:Interleukin-1beta; D015847:Interleukin-4