CE-ESI-TOF/MS for human growth hormone analysis.
CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE-ESI-TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples.
Staub
Aline
A
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
Giraud
Sylvain
S
Saugy
Martial
M
Rudaz
Serge
S
Veuthey
Jean-Luc
JL
Schappler
Julie
J
D000097:Acetonitriles / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D019382:Human Growth Hormone / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000097:Acetonitriles; D019382:Human Growth Hormone; C032159:acetonitrile