Indirect immunofluorescent labeling of viable cells.
A recognized flow cytometry procedure for standard indirect antibody staining for cellular analysis and cell sorting is discussed. A protocol for cell surface or intracellular antibody staining of viable cells is provided.
Cunningham
Robert E
RE
Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005456:Fluorescent Dyes