Monitoring neuropeptides in vivo via microdialysis and mass spectrometry.
Neuropeptides are important signaling molecules that regulate many essential physiological processes. Microdialysis offers a way to sample neuropeptides in vivo. When combined with liquid chromatography-mass spectrometry detection, many known and unknown neuropeptides can be identified from a live organism. This chapter describes sample preparation techniques and general strategies for the mass spectral analysis of neuropeptides collected via microdialysis sampling. Methods for the in vitro microdialysis of a neuropeptide standard as well as the in vivo microdialysis sampling of neuropeptides from a live crab are described.
Behrens
Heidi L
HL
Department of Chemistry & School of Pharmacy, University of Wisconsin-Madison, 53705-2222, Madison, WI, USA. lli@pharmacy.wisc.edu
Li
Lingjun
L
D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000033:anatomy & histology / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*
D009479:Neuropeptides