Label-free dual assay of DNA sequences and potassium ions using an aptamer probe and a molecular light switch complex.
A homogeneous assay is reported using Ru(phen)(2)(dppz)(2+) and a K(+)-binding aptamer for the selective and sensitive detection of a target oligonucleotide and potassium ions, based on reduction in fluorescence emission according to the formation of the G-quadruplex structure from the aptamer in the presence of K(+).
Choi
Min Sun
MS
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Yoon
Minji
M
Baeg
Jin-Ook
JO
Kim
Jinheung
J
D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D054856:G-Quadruplexes; D011188:Potassium / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D052157:Aptamers, Nucleotide; D002412:Cations; D011188:Potassium