title: Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.
abstract: To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.
authors.affiliation: USDA-ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA. jeff.lord@ars.usda.gov
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Lord
authors.forename: Kris
authors.initials: K
authors.lastname: Hartzer
authors.forename: Michelle
authors.initials: M
authors.lastname: Toutges
authors.forename: Brenda
authors.initials: B
authors.lastname: Oppert
mesh_terms: D000818:Animals; D052982:Beauveria / Q000276:immunology* / Q000472:pathogenicity*; D020869:Gene Expression Profiling / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012015:Reference Standards; D014231:Tribolium / Q000235:genetics* / Q000382:microbiology