Effects of glyoxal or methylglyoxal on the metabolism of amino acids, lactate, glucose and acetate in the cerebral cortex of young and adult rats.
The in vitro effects of glyoxal and methylglyoxal on the metabolism of glycine, alanine, leucine, glutamate, glutamine, glucose, lactate and acetate were evaluated in cortico-cerebral slices from young (10-day-old) or adult (3-month-old) rats. In a first set of experiments with cortico-cerebral slices from young animals, the compounds glyoxal or methylglyoxal at 400 microM, increased the oxidation of alanine, leucine and glycine to CO(2) and decreased the protein synthesis from these amino acids. Lipid synthesis from alanine, leucine and glycine was not changed in the cortico-cerebral slices from young rats after glyoxals exposure. Moreover, glutamine oxidation to CO(2) decreased by glyoxals exposure, but glutamate oxidation was not affected. In a second set of experiments with brain slices from adult animals, glycine metabolism (oxidation to CO(2), conversion to lipids or incorporation into proteins) was not changed by glyoxals exposure. In addition, the oxidation rates of glucose, lactate, acetate, glutamine and glutamate to CO(2) were also not modified. Taken together, these results indicate that glyoxal disrupts the energetic metabolism of the rat cerebral cortex in vitro. However, only young animals were susceptible to such events, suggesting that the immature cerebral cortex is less capable of dealing with glyoxal than the mature one.
Schmidt
Betina
B
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 Anexo, 90035-003, Porto Alegre, RS, Brazil.
de Assis
Adriano Martimbianco
AM
Battu
Cíntia Eickhoff
CE
Rieger
Débora Kurle
DK
Hansen
Fernanda
F
Sordi
Fernanda
F
Longoni
Aline
A
Hoefel
Ana Lúcia
AL
Farina
Marcelo
M
Gonçalves
Carlos Alberto
CA
Souza
Diogo Onofre
DO
Perry
Marcos Luiz Santos
ML
D000085:Acetates / Q000378:metabolism; D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006037:Glyoxal / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects; D011765:Pyruvaldehyde / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D000596:Amino Acids; D002491:Central Nervous System Agents; D005973:Glutamine; D002245:Carbon Dioxide; D019344:Lactic Acid; D018698:Glutamic Acid; D006037:Glyoxal; D011765:Pyruvaldehyde; D005947:Glucose; D005998:Glycine