Modulation of monoamine neurotransmitters in fighting fish Betta splendens exposed to waterborne phytoestrogens.
Endogenous estrogens are known to affect the activity of monoamine neurotransmitters in vertebrate animals, but the effects of exogenous estrogens on neurotransmitters are relatively poorly understood. We exposed sexually mature male fighting fish Betta splendens to environmentally relevant and pharmacological doses of three phytoestrogens that are potential endocrine disruptors in wild fish populations: genistein, equol, and β-sitosterol. We also exposed fish to two doses of the endogenous estrogen 17β-estradiol, which we selected as a positive control because phytoestrogens are putative estrogen mimics. Our results were variable, but the effects were generally modest. Genistein increased dopamine levels in the forebrains of B. splendens at both environmentally relevant and pharmacological doses. The environmentally relevant dose of equol increased dopamine levels in B. splendens forebrains, and the pharmacological dose decreased norepinephrine (forebrain), dopamine (hindbrain), and serotonin (forebrain) levels. The environmentally relevant dose of β-sitosterol decreased norepinephrine and dopamine in the forebrain and hindbrain, respectively. Our results suggest that sources of environmental phytoestrogens, such as runoff or effluent from agricultural fields, wood pulp mills, and sewage treatment plants, have the potential to modulate neurotransmitter activity in free-living fishes in a way that could interfere with normal behavioral processes.
Clotfelter
Ethan D
ED
Department of Biology, Amherst College, Amherst, MA 01002, USA. edclotfelter@amherst.edu
McNitt
Meredith M
MM
Carpenter
Russ E
RE
Summers
Cliff H
CH
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism*; D060754:Equol; D019833:Genistein; D007529:Isoflavones; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D048789:Phytoestrogens / Q000378:metabolism / Q000633:toxicity*; D012701:Serotonin / Q000378:metabolism*; D012855:Sitosterols; D050493:Vesicular Monoamine Transport Proteins / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007529:Isoflavones; D048789:Phytoestrogens; D012855:Sitosterols; D050493:Vesicular Monoamine Transport Proteins; D014874:Water Pollutants, Chemical; D012701:Serotonin; D060754:Equol; C025473:gamma-sitosterol; D019833:Genistein; D004298:Dopamine; D009638:Norepinephrine