[Study on the differentially expressed genes of steroid-resistant and steroid-sensitive nephrotic syndrome].
OBJECTIVE
To identify the genes differentially expressed among the steroid-resistant nephrotic syndrome (SRNS), steroid-sensitive nephrotic syndrome (SSNS) and normal children, and understand the molecular mechanism of SRNS.


METHODS
Affymetrix microarray technology was used to obtain such a profile. The differentially expressed genes among these groups were identified based on signal-to-noise ratios by GCOS software; real-time PCR analysis was performed to confirm the microarray results.


RESULTS
There were 157 genes differentially expressed among these groups. The genes up-regulated both in SRNS and SSNS were involved primarily in ionic transportation, immuno-signal transduction and apoptosis. In particular, CLNS1A gene was down regulated in SRNS but up regulated in SSNS.


CONCLUSIONS
Several differentially expressed genes, such as CLNS1A and HLA-DRB4 were found to be closely related to the pathogenesis of SRNS and SSNS. This DNA microarray analysis has provided some important clue to the molecular mechanism of SRNS.
Chen
Rong-yan
RY
Department of Pediatrics, the Affiliated Guangzhou Municipal People's Hospital of Guangzhou Medical College, Guangzhou, Guangdong, 510180 P.R. China.
Yu
Li
L
Hao
Zhi-hong
ZH
Wang
Li-na
LN
Wen
Yue-qiang
YQ
D002648:Child; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000235:genetics*; D013256:Steroids / Q000627:therapeutic use*
D013256:Steroids