Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates.
This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.
Kim
Young-Pil
YP
Molecular Imaging Program at Stanford, Department of Radiology and Chemistry, Stanford University, Stanford, CA 94305-5484, USA.
Daniel
Weston L
WL
Xia
Zuyong
Z
Xie
Hexin
H
Mirkin
Chad A
CA
Rao
Jianghong
J
D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000737:chemistry*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D009154:Mutation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D049408:Luminescent Agents; D011994:Recombinant Proteins; D006046:Gold; D008156:Luciferases; D020778:Matrix Metalloproteinase 2