Application of microarray-based analysis of gene expression in the field of toxicogenomics.
The field of toxicogenomics, which is becoming an important sub-discipline of toxicology, resulted from the natural convergence of the field of conventional toxicological research and the emergent field of functional genomics. One technology that has played a significant role in the field of toxicogenomics (in addition to many others) is the gene expression microarray. In this chapter, the authors provide an example of the application of gene expression microarrays to the field of toxicogenomics by detailing the strategy that was used for obtaining, analyzing, and interpreting gene expression data generated from RNA isolated from the liver of toxicant-exposed rats.
Mei
Nan
N
Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, Jefferson, AR, USA.
Fuscoe
James C
JC
Lobenhofer
Edward K
EK
Guo
Lei
L
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D009153:Mutagens / Q000494:pharmacology; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D043922:Toxicogenetics / Q000379:methods*
D009153:Mutagens; D012313:RNA