title: Modeling withdrawal syndrome in zebrafish.
abstract: The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscience research. Zebrafish behavior is robustly affected by environmental and pharmacological manipulations, and can be examined using exploration-based paradigms, paralleled by analysis of endocrine (cortisol) stress responses. Discontinuation of various psychotropic drugs evokes withdrawal in both humans and rodents, characterized by increased anxiety. Sensitivity of zebrafish to drugs of abuse has been recently reported in the literature. Here we examine the effects of ethanol, diazepam, morphine and caffeine withdrawal on zebrafish behavior. Overall, discontinuation of ethanol, diazepam and morphine produced anxiogenic-like behavioral or endocrine responses, demonstrating the utility of zebrafish in translational research of withdrawal syndrome.
authors.affiliation: Department of Pharmacology and Neuroscience Program, Tulane University Medical School, New Orleans, LA 70112, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cachat
authors.forename: Peter
authors.initials: P
authors.lastname: Canavello
authors.forename: Marco
authors.initials: M
authors.lastname: Elegante
authors.forename: Brett
authors.initials: B
authors.lastname: Bartels
authors.forename: Peter
authors.initials: P
authors.lastname: Hart
authors.forename: Carisa
authors.initials: C
authors.lastname: Bergner
authors.forename: Rupert
authors.initials: R
authors.lastname: Egan
authors.forename: Ashley
authors.initials: A
authors.lastname: Duncan
authors.forename: David
authors.initials: D
authors.lastname: Tien
authors.forename: Amanda
authors.initials: A
authors.lastname: Chung
authors.forename: Keith
authors.initials: K
authors.lastname: Wong
authors.forename: Jason
authors.initials: J
authors.lastname: Goodspeed
authors.forename: Julia
authors.initials: J
authors.lastname: Tan
authors.forename: Chelsea
authors.initials: C
authors.lastname: Grimes
authors.forename: Salem
authors.initials: S
authors.lastname: Elkhayat
authors.forename: Christopher
authors.initials: C
authors.lastname: Suciu
authors.forename: Michael
authors.initials: M
authors.lastname: Rosenberg
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Chung
authors.forename: Ferdous
authors.initials: F
authors.lastname: Kadri
authors.forename: Sudipta
authors.initials: S
authors.lastname: Roy
authors.forename: Siddharth
authors.initials: S
authors.lastname: Gaikwad
authors.forename: Adam
authors.initials: A
authors.lastname: Stewart
authors.forename: Ivan
authors.initials: I
authors.lastname: Zapolsky
authors.forename: Thomas
authors.initials: T
authors.lastname: Gilder
authors.forename: Sopan
authors.initials: S
authors.lastname: Mohnot
authors.forename: Esther
authors.initials: E
authors.lastname: Beeson
authors.forename: Hakima
authors.initials: H
authors.lastname: Amri
authors.forename: Zofia
authors.initials: Z
authors.lastname: Zukowska
authors.forename: R Denis
authors.initials: RD
authors.lastname: Soignier
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
mesh_terms: D000818:Animals; D001007:Anxiety / Q000209:etiology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002110:Caffeine / Q000009:adverse effects; D003975:Diazepam / Q000009:adverse effects; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects; D005106:Exploratory Behavior; D005260:Female; D006854:Hydrocortisone; D008297:Male; D009020:Morphine / Q000009:adverse effects; D013375:Substance Withdrawal Syndrome / Q000097:blood / Q000150:complications* / Q000209:etiology*; D013997:Time Factors; D015027:Zebrafish
chemical_list: D002110:Caffeine; D000431:Ethanol; D009020:Morphine; D003975:Diazepam; D006854:Hydrocortisone