Cleavable linker for photo-cross-linked small-molecule affinity matrix.
The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.
Kanoh
Naoki
N
Tohoku University, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kanoh@mail.pharm.tohoku.ac
Takayama
Hiroshi
H
Honda
Kaori
K
Moriya
Takashi
T
Teruya
Takayuki
T
Simizu
Siro
S
Osada
Hiroyuki
H
Iwabuchi
Yoshiharu
Y
D001665:Binding Sites; D015153:Blotting, Western; D002853:Chromatography, Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D013058:Mass Spectrometry; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry*; D010777:Photochemistry; D011485:Protein Binding; D054648:Protein Phosphatase 2 / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry
D003432:Cross-Linking Reagents; D004220:Disulfides; D004800:Enzymes, Immobilized; D008024:Ligands; D009943:Organophosphorus Compounds; D019920:Photoaffinity Labels; C030011:carbene; D012685:Sepharose; D054648:Protein Phosphatase 2; D008697:Methane