title: Tooth replantation after use of Euro-Collins solution or bovine milk as storage medium: a histomorphometric analysis in dogs.
abstract: OBJECTIVE
  Euro-Collins solution was developed for the preservation of organs for transplantation, whose characteristics have raised interest for its use as a storage medium for avulsed teeth before replantation. This study evaluated histologically and morphometrically the healing process of dog teeth replanted after storage in Euro-Collins solution or bovine milk.
  
  
  METHODS
  Eighty roots of 4 young adult mongrel dogs were randomly assigned to 4 groups (n = 20) and the root canals were instrumented and obturated with gutta-percha and a calcium hydroxide-based sealer. After 2 weeks, the teeth were extracted and subjected to the following protocols: GI (negative control), replantation immediately after extraction; GII (positive control), bench-drying for 2 hours before replantation; GIII and GIV, immersion in 10 mL of whole bovine milk and Euro-Collins solution at 4 degrees C, respectively, for 8 hours before replantation. The animals were sacrificed 90 days postoperatively. The pieces containing the replanted teeth were subjected to routine processing for histologic and histometric analyses under light microscopy and polarized light microscopy.
  
  
  RESULTS
  Root resorption was observed in all groups. GII exhibited the greatest loss of dental structure (P < .01), and inflammatory resorption was predominant in this group. Storage in milk showed poorer results than immediate replantation and storage in Euro-Collins solution (P < .01). The teeth stored in Euro-Collins solution presented similar extension of root resorption and periodontal ligament reorganization to those of immediately replanted teeth.
  
  
  CONCLUSIONS
  The findings of this study suggest that the Euro-Collins solution is an adequate storage medium for keeping avulsed teeth for up to 8 hours before replantation.
authors.affiliation: Oral and Maxillofacial Surgery and Traumatology, Dental School of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
authors.forename: André Dotto
authors.initials: AD
authors.lastname: Sottovia
authors.forename: Dagoberto
authors.initials: D
authors.lastname: Sottovia Filho
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Poi
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Panzarini
authors.forename: Danielle Shima
authors.initials: DS
authors.lastname: Luize
authors.forename: Celso Koogi
authors.initials: CK
authors.lastname: Sonoda
mesh_terms: D000818:Animals; D003804:Dentin / Q000473:pathology; D004285:Dogs; D006982:Hypertonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D012391:Root Resorption / Q000473:pathology; D014084:Tooth Avulsion / Q000473:pathology; D014090:Tooth Replantation; D014092:Tooth Root / Q000473:pathology
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions