title: Identification of zoonotic Giardia genotypes in fish.
abstract: Apart from a single record in a shark, there have been no published studies conducted on Giardia genotypes in fish. The present study investigated the prevalence of Giardia in cultured fingerlings (n=227), wild freshwater (n=227) and wild marine/estuarine species (n=255) of fish in Western Australia by PCR amplification at the 18S rRNA, glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and beta-giardin (bg) loci. Results revealed a low prevalence of Giardia, 3.8% (27/709), in fish hosts. The zoonotic Giardia species, Giardia duodenalis assemblages A, B as well as G. duodenalis assemblage E and Giardia microti were detected. The identification of zoonotic species of Giardia highlights the public health importance of investigating parasites within fish host species.
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.forename: Anna
authors.initials: A
authors.lastname: Reid
authors.forename: Alan
authors.initials: A
authors.lastname: Lymbery
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003598:Cytoskeletal Proteins; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology*; D005399:Fishes; D020459:Genes, rRNA; D005838:Genotype; D005872:Giardia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014305:Triose-Phosphate Isomerase / Q000235:genetics; D014904:Western Australia
chemical_list: D003598:Cytoskeletal Proteins; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; C038207:giardin protein, Giardia lamblia; D005969:Glutamate Dehydrogenase; D014305:Triose-Phosphate Isomerase