title: L-Cysteine capped CdTe-CdS core-shell quantum dots: preparation, characterization and immuno-labeling of HeLa cells.
abstract: Functionalized CdTe-CdS core-shell quantum dots (QDs) were synthesized in aqueous solution via water-bathing combined hydrothermal method using L-cysteine (L-Cys) as a stabilizer. This method possesses both the advantages of water-bathing and hydrothermal methods for preparing high-quality QDs with markedly reduced synthesis time, and better stability than a lone hydrothermal method. The QDs were characterized by transmission electronic microscopy and powder X-ray diffraction and X-ray photoelectron spectroscopy. The CdTe-CdS QDs with core-shell structure showed both enhanced fluorescence and better photo stability than nude CdTe QDs. After conjugating with antibody rabbit anti-CEACAM8 (CD67), the as-prepared l-Cys capped CdTe-CdS QDs were successfully used as fluorescent probes for the direct immuno-labeling and imaging of HeLa cells. It was indicated that this kind of QD would have application potential in bio-labeling and cell imaging.
authors.affiliation: Department of Chemistry, Northeastern University, Shenyang 110004, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Pan
authors.initials: P
authors.lastname: Sun
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Huanyu
authors.initials: H
authors.lastname: Gao
authors.forename: Linru
authors.initials: L
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Fang
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Jinling
authors.initials: J
authors.lastname: Liu
authors.forename: Shukun
authors.initials: S
authors.lastname: Xu
mesh_terms: D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots; D013440:Sulfides / Q000138:chemical synthesis / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D003545:Cysteine; D013691:Tellurium; C028337:cadmium telluride