The effect of epigallocatechin-3-gallate, a constituent of green tea, on transforming growth factor-beta1-stimulated wound contraction.
Dermal fibrosis, or scarring, following surgical incisions, traumatic wounds and burns presents a major clinical burden. Transforming growth factor (TGF)-beta1 is a major factor known to stimulate fibroblast proliferation, collagen production, and the differentiation of fibroblast to myofibroblast promoting wound contraction. Furthermore, excessive or prolonged TGF-beta1 has been shown to be associated with scarring. Green tea contains high amounts of polyphenols with the major polyphenolic compound being epigallocatechin-3-gallate (EGCG). EGCG has been shown to be anti-inflammatory, anti-oxidant, and may improve wound healing and scarring, though its precise effect on TGF-beta1 remains unclear. This study aimed at determining the effect of EGCG on TGF-beta1 collagen contraction, gene expression and the differentiation of fibroblast to myofibroblast. EGCG appears to affect the role that TGF-beta1 plays in fibroblast populated collagen gel contraction and this seems to be through both myofibroblast differentiation and connective tissue growth factor gene expression and reduces the expression of collagen type I gene regulation.
Klass
Benjamin R
BR
The RAFT Institute, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom.
Branford
Olivier A
OA
Grobbelaar
Adriaan O
AO
Rolfe
Kerstin J
KJ
D000199:Actins / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000166:cytology; D013662:Tea / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
C541116:ACTA2 protein, human; D000199:Actins; D010936:Plant Extracts; D013662:Tea; D053773:Transforming Growth Factor beta1; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate; D020782:Matrix Metalloproteinases