An immunochemical test for rapid screening of zearalenone and T-2 toxin.
An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 microg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.
Basova
Evgenia Yu
EY
Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
Goryacheva
Irina Yu
IY
Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia. Goryachevaiy@mail.ru.
Rusanova
Tatiana Yu
TY
Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
Burmistrova
Natalia A
NA
Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
Dietrich
Richard
R
Chair of Hygiene and Technology of Milk, Ludwig-Maximilians-Universität München, Schonleutnerstrasse 8, 85764, Oberschleißheim, Germany.
Märtlbauer
Erwin
E
Chair of Hygiene and Technology of Milk, Ludwig-Maximilians-Universität München, Schonleutnerstrasse 8, 85764, Oberschleißheim, Germany.
Detavernier
Christ'l
C
Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
Van Peteghem
Carlos
C
Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
De Saeger
Sarah
S
Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D055910:Antibodies, Immobilized; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques; D013058:Mass Spectrometry; D013605:T-2 Toxin / Q000032:analysis*; D014118:Toxins, Biological / Q000032:analysis*; D014908:Triticum / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
D055910:Antibodies, Immobilized; D014118:Toxins, Biological; D015025:Zearalenone; D013605:T-2 Toxin