Spectrophotometric determination of dopamine hydrochloride in pharmaceutical, banana, urine and serum samples by potassium ferricyanide-Fe(III).
In the present work, we developed a simple, sensitive and inexpensive method to determine dopamine hydrochloride using potassium ferricyanide-Fe(III) by spectrophotometry. The results show that Fe(III) is deoxidized to Fe(II) by dopamine hydrochloride at pH 4.0, and then Fe(II) reacts with potassium ferricyanide to form a soluble prussian blue (KFe(III)[Fe(II)(CN)6]). The absorbance of this product was monitored over time using a spectrophotometer at an absorption maximum of 735 nm, and the amount of dopamine hydrochloride could be calculated based on the absorbance. A good linear relationship of the concentration of dopamine hydrochloride versus absorbance was observed, and a linear regression equation of A = 0.022 + 0.16921C (microg mL(-1)) was obtained. Moreover, the apparent molar absorption coefficient for the indirect determination of dopamine hydrochloride was 3.2 x 10(4) L mol(-1) cm(-1). This described method has been used to determine dopamine hydrochloride in pharmaceutical, banana, urine and serum samples with satisfactory results.
Guo
Li
L
College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, P. R. China.
Zhang
Yan
Y
Li
Quanmin
Q
D000042:Absorption; D000143:Acids / Q000737:chemistry; D002138:Calibration; D004298:Dopamine / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D005292:Ferricyanides / Q000737:chemistry*; D006801:Humans; D007267:Injections; D007700:Kinetics; D057230:Limit of Detection; D028521:Musa / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000191:economics / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000143:Acids; D005292:Ferricyanides; D004364:Pharmaceutical Preparations; D012997:Solvents; C028033:potassium ferricyanide; D004298:Dopamine