[Application of multicolor primed in situ labeling in simultaneous identification of chromosomes 18, X and Y in uncultured amniocytes].
OBJECTIVE
To establish a multicolor primed in situ labeling (PRINS) protocol for chromosome detection in uncultured amniocytes.


METHODS
Chromosomes 18, X and Y in uncultured amniocytes were simultaneously detected by using the non-ddNTP-blocking multicolor PRINS procedure.


RESULTS
Within 7 h, the 3 chromosomes were simultaneously marked in the same uncultured amniocyte. The chromosome signals were successfully detected in 69 uncultured samples of amniotic fluid. The results were consistent with that obtained by chromosomes in cultured amniocytes.


CONCLUSIONS
This multicolor protocol was high throughput, fast, simple, sensitive and reliable in diagnosing chromosome abnormalities in uncultured amniocytes.
Deng
Li
L
Institute of Medical Genetics, Sichuan University School of Life Science, Key Laboratory of Bioresources and Eco-environment, Ministry of Education, Chengdu, Sichuan, 610064 P.R. China.
Ding
Xian-ping
XP
Nie
Yong
Y
Hu
Yu
Y
Ren
Hong-jing
HJ
D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry* / Q000166:cytology; D002478:Cells, Cultured; D002887:Chromosomes, Human, Pair 18 / Q000737:chemistry* / Q000235:genetics; D041321:Chromosomes, Human, X / Q000737:chemistry* / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000737:chemistry* / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D020401:Primed In Situ Labeling / Q000379:methods*; D055815:Young Adult