Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.
Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of 2-HP-beta-CD and the results gave strong indications on the non-equilibrium nature of the system. The globule-to-coil transition was not found to proceed through a coexistence region, which seems to be a general phenomenon in DNA decompaction using CDs.
Carlstedt
Jonas
J
Physical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100 Lund, Sweden. jonas.carlstedt@fkem1.lu.se
González-Pérez
Alfredo
A
Alatorre-Meda
Manuel
M
Dias
Rita S
RS
Lindman
Björn
B
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D017122:Bacteriophage T4 / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013016:Sound; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
D004279:DNA, Viral; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin