title: Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.
abstract: Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3'-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ayyamaji@mail.ecc.u-tokyo.ac.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Keitaro
authors.initials: K
authors.lastname: Sakurai
authors.forename: Koji
authors.initials: K
authors.lastname: Hamada
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.forename: Johji
authors.initials: J
authors.lastname: Ozeki
authors.forename: Akiko
authors.initials: A
authors.lastname: Yoshida
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yoshii
authors.forename: Takumi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Hibi
mesh_terms: D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors; D054884:Host-Pathogen Interactions; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000235:genetics; D014176:Protein Biosynthesis; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*
chemical_list: D020648:Peptide Elongation Factor 1