Vascular endothelial growth factor polymorphisms in mantle cell lymphoma.
In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C-460T, C+936T and C-2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGFC-460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C-2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation.
Galimberti
S
S
Department of Oncology, Transplant, and Advances in Medicine Hematology Section, University of Pisa, Ospedale S. Chiara Via Roma 56, IT-56126 Pisa, Italy. s.galimberti @ med.unipi.it
Nagy
B
B
Palumbo
G A
GA
Ciancia
E
E
Buda
G
G
Orciuolo
E
E
Melosi
A
A
Lambelet
P
P
Ronca
F
F
Petrini
M
M
D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D042461:Vascular Endothelial Growth Factor A