title: Delivery of cloned offspring: experience in Zebu cattle (Bos indicus).
abstract: The production of a healthy cloned calf is dependent on a multitude of successful steps, including reprogramming mediated by the oocyte, the development of a functional placenta, adequate maternal-fetal interaction, the establishment of a physiological metabolic setting and the formation of a complete set of well-differentiated cells that will eventually result in well-characterised and fully competent tissues and organs. Although the efficiency of nuclear transfer has improved significantly since the first report of a somatic cell nuclear transfer-derived animal, there are many descriptions of anomalies concerning cloned calves leading to high perinatal morbidity and mortality. The present article discusses some our experience regarding perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has led to improved survival rates in Nellore cloned calves following the application of such 'labour-intensive technology'.
authors.affiliation: Faculty of Animal Sciences and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225. Pirassununga, SP 13635-900, Brazil. meirellf@usp.br
authors.forename: Flávio V
authors.initials: FV
authors.lastname: Meirelles
authors.forename: Eduardo H
authors.initials: EH
authors.lastname: Birgel
authors.forename: Felipe
authors.initials: F
authors.lastname: Perecin
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bertolini
authors.forename: Anneliese S
authors.initials: AS
authors.lastname: Traldi
authors.forename: José Rodrigo V
authors.initials: JR
authors.lastname: Pimentel
authors.forename: Eliza R
authors.initials: ER
authors.lastname: Komninou
authors.forename: Juliano R
authors.initials: JR
authors.lastname: Sangalli
authors.forename: Paulo Fantinato
authors.initials: PF
authors.lastname: Neto
authors.forename: Mariana Tikuma
authors.initials: MT
authors.lastname: Nunes
authors.forename: Fábio Celidonio
authors.initials: FC
authors.lastname: Pogliani
authors.forename: Flávia D P
authors.initials: FD
authors.lastname: Meirelles
authors.forename: Flávia S
authors.initials: FS
authors.lastname: Kubrusly
authors.forename: Camila I
authors.initials: CI
authors.lastname: Vannucchi
authors.forename: Liege C G
authors.initials: LC
authors.lastname: Silva
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle / Q000196:embryology*; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D000013:Congenital Abnormalities / Q000401:mortality / Q000662:veterinary; D005260:Female; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D015996:Survival Rate