Efficient protein renaturation using tunable hemifluorinated anionic surfactants as additives.
We show that hemifluorinated anionic surfactants are effective as renaturing additives for bovine carbonic anhydrase (CAB) below their critical micelle concentration (CMC) providing high refolding yields for thermally and chemically denatured protein.
Singh
Rajni
R
Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA.
Flowers
Robert A
RA
D000818:Animals; D000838:Anions / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry*; D002417:Cattle; D002942:Circular Dichroism; D054879:Halogenation; D008823:Micelles; D017510:Protein Folding; D020673:Protein Renaturation; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature
D000838:Anions; D008823:Micelles; D013501:Surface-Active Agents; D002256:Carbonic Anhydrases