title: Osteogenic potential of autogenous bone associated with bone marrow osteoblastic cells in bony defects: a histomorphometric study.
abstract: OBJECTIVE
  Because of limitations of autogenous grafts, allografts, xenografts, alloplasts, and hydroxyapatite as graft materials, researchers have been using bone tissue engineering as a strategy for bone regeneration. The aim of this work was to study the effect of bone tissue engineering, associating bone marrow osteoblastic cells, and autogenous bone in defects created by dental extraction in rats.
  
  
  METHODS
  Eighty male rats from 250 to 300 g were anesthetized, submitted to the extraction of the superior incisor, and divided in control group (C), implanted with osteoblastic cells (OC), autogenous bone (AB), and osteoblastic cells + autogenous bone (OC + AB). The animals were killed on 10th and 20th days after surgery and their maxilla were processed for obtaining fine semiserial sections (5 mum), and then stained with hematoxylin-eosin. Through image analysis system, bone volume in areas adjacent to the implants was estimated.
  
  
  RESULTS
  The histometric results revealed that the association OC + AB produced significant increase (10%-15%) of bone in both experimental periods when compared with the control group (P < or = 0.01).
  
  
  CONCLUSIONS
  Osteoblastic cells associated with autogenous bone accelerated the repair of bone defect, and the action of the osteoblastic cells was more effective until the 10th day and of the autogenous bone after this period.
authors.affiliation: Department of Morphology, Stomatology and Physiology, Dentistry School, University of São Paulo-USP, 14040-904, Ribeirão Preto, São Paulo, Brazil. suzie@forp.usp.br
authors.forename: Suzie Aparecida
authors.initials: SA
authors.lastname: Lacerda
authors.forename: João Fernando Arnoni
authors.initials: JF
authors.lastname: Lanzoni
authors.forename: Karina Fittipaldi
authors.initials: KF
authors.lastname: Bombonato-Prado
authors.forename: Alessandra Aparecida
authors.initials: AA
authors.lastname: Campos
authors.forename: Celina Antonio
authors.initials: CA
authors.lastname: Prata
authors.forename: Luiz Guilherme
authors.initials: LG
authors.lastname: Brentegani
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000473:pathology*; D001857:Bone Matrix / Q000473:pathology; D001861:Bone Regeneration / Q000502:physiology; D016025:Bone Transplantation / Q000473:pathology*; D002478:Cells, Cultured; D003238:Connective Tissue / Q000473:pathology; D008297:Male; D008439:Maxillary Diseases / Q000601:surgery*; D010006:Osteoblasts / Q000637:transplantation*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014081:Tooth Extraction / Q000009:adverse effects; D014945:Wound Healing / Q000502:physiology