title: Chk1-cyclin A/Cdk1 axis regulates origin firing programs in mammals.
abstract: DNA replication is key to ensuring the complete duplication of genomic DNA prior to mitosis and is tightly regulated by both cell cycle machinery and checkpoint signals. Regulation of the S phase program occurs at several stages, affecting origin firing, replication fork elongation, fork velocity, and fork stability, all of which are dependent on S-phase-promoting kinase activity. Somatic mammalian cells use well-established origin programs by which specific regions of the genome are replicated at precise times. However, the mechanisms by which S phase kinases regulate origin firing in mammals are largely unknown. Here, we discuss recent advances in the understanding of how S phase programs are regulated in mammals at the correct regions and at the appropriate times.
authors.affiliation: Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan. mkt-naka@med.nagoya-cu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katsuno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Niida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Midori
authors.initials: M
authors.lastname: Shimada
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D000071877:Checkpoint Kinase 1; D019925:Cyclin A / Q000378:metabolism*; D008322:Mammals / Q000235:genetics*; D011494:Protein Kinases / Q000378:metabolism*; D018741:Replication Origin; D016196:S Phase
chemical_list: D019925:Cyclin A; D011494:Protein Kinases; D000071877:Checkpoint Kinase 1; D016203:CDC2 Protein Kinase