Synthesis and DNA-cleavage activities of dinuclear copper(II) complexes of urea-bridged macrocyclic polyamines.
Two dinuclear macrocyclic polyamine copper(II) (Cu(II)) complexes, which have two cyclen units linked by urea, were synthesized as DNA-cleavage agents. The structures of these new dinuclear complexes were identified by HR-ESI-MS and IR analyses. The catalytic activities of DNA cleavage of these dinuclear Cu(II) complexes were subsequently studied. The results show that 6a was the better catalyst in the DNA-cleavage process than 6b. The effects of reaction time and concentration of complexes were also investigated. The results indicate, that the Cu(II) complexes could catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA; Form I) under physiological conditions to produce selectively nicked DNA (Form II; no Form III was produced) with high yields (nearly 100%) in short time in the absence of reductant or oxidant.
Yue
Yang
Y
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, P. R. China.
Li
Jing
J
Zhang
Ji
J
Zhang
Zhong-Wei
ZW
Lin
Hong-Hui
HH
Chen
Shan-Yong
SY
Yu
Xiao-Qi
XQ
D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D053837:DNA Cleavage / Q000187:drug effects*; D047028:Macrocyclic Compounds / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D014508:Urea / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
D056831:Coordination Complexes; D047028:Macrocyclic Compounds; D009942:Organometallic Compounds; D011073:Polyamines; D003300:Copper; D014508:Urea