[Quantitative assessment of in vitro neutralizing activity of secretory factors from rat lungs against influenza virus].
Secretory factors were isolated by lung wash followed by centrifugation to remove cells, dialysis of supernatant to remove NaCl salt, lyophilization of the lavage fluid and resuspention of the lyophilization product in an isotonic NaCl solution. It was shown that biological activity of influenza virus /Aichi/2/68 (3N2) significantly decreased (p = 0,01) from 8,17 +/- 0,10 to 7,14 +/- 0,20 IgEID50/ml during its incubation with secretory factors at 37 degrees C for 1 hr and to 7,92 +/- 0,17 IgEID50/ml in isotonic NaCl solution in the absence of these factors. Their concentration in the incubation medium was estimated to be 9.1 +/- 0.7% of their level in the lungs.
Zhukov
V A
VA
Shishkina
L N
LN
Sergeev
A A
AA
Pliasunov
I V
IV
P'iankov
O V
OV
Petrishchenko
V A
VA
Sergeev
A N
AN
Zaĭtsev
B N
BN
Nesvizhskiĭ
Iu V
IuV
Vorob'ev
A A
AA
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D053122:Influenza A Virus, H3N2 Subtype / Q000472:pathogenicity*; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000821:virology; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000821:virology
D011663:Pulmonary Surfactants