Sulfation of tea polysaccharides: synthesis, characterization and hypoglycemic activity.
Neutral polysaccharides (NTPS) and acid polysaccharides (ATPS) from tea leaves were obtained on a D315 macroporous anion-exchange resin column chromatography. NTPS and ATPS were sulfated by the pyridine-sulfonic acid method to obtain NTPS-S and ATPS-S. It was found that NTPS was easier sulfated than ATPS. There are strong characteristic absorption peaks located in 1258 cm(-1), 1146 cm(-1), 832 cm(-1) and 617 cm(-1) in the FTIR spectra of sulfated polysaccharides. Sulfation of polysaccharides also affected the endothermic and exothermic peaks via the DSC scan analysis. The appearance of exothermic peaks in both NTPS-S and ATPS-S indicated that the redox reaction might happen. The comparative study of hypoglycemic effect on mice showed that the sulfation of polysaccharides significantly improved hypoglycemic activity.
Wang
Yuanfeng
Y
College of Life and Environment Science, Shanghai Normal University, Shanghai, China.
Peng
Yonghua
Y
Wei
Xinlin
X
Yang
Zhiwei
Z
Xiao
Jianbo
J
Jin
Zhengyu
Z
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002236:Carbohydrate Conformation; D007003:Hypoglycemia / Q000188:drug therapy*; D008297:Male; D051379:Mice; D018625:Microscopy, Atomic Force; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use*; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000378:metabolism*; D013662:Tea / Q000737:chemistry*
D001786:Blood Glucose; D011134:Polysaccharides; D013431:Sulfates; D013662:Tea