title: Rotavirus infection activates dendritic cells from Peyer's patches in adult mice.
abstract: This study used an in vivo mouse model to analyze the response of dendritic cells (DCs) in Peyer's patches (PPs) within the first 48 h of infection with the wild-type murine rotavirus EDIM (EDIM(wt)). After the infection, the absolute number of DCs was increased by 2-fold in the PPs without a modification of their relative percentage of the total cell number. Also, the DCs from PPs of infected mice showed a time-dependent migration to the subepithelial dome (SED) and an increase of the surface activation markers CD40, CD80, and CD86. This response was more evident at 48 h postinfection (p.i.) and depended on viral replication, since DCs from PPs of mice inoculated with UV-treated virus did not show this phenotype. As a result of the activation, the DCs showed an increase in the expression of mRNA for the proinflammatory cytokines interleukin-12/23p40 (IL-12/23p40), tumor necrosis factor alpha (TNF-alpha), and beta interferon (IFN-beta), as well as for the regulatory cytokine IL-10. These results suggest that, a short time after rotavirus infection, the DCs from PPs play a critical role in controlling the infection and, at the same time, avoiding an excessive inflammatory immune response.
authors.affiliation: Facultad de Medicina, Universidad Autonoma del Estado de Morelos, 62210 Morelos, Mexico.
authors.forename: Delia V
authors.initials: DV
authors.lastname: Lopez-Guerrero
authors.forename: Selene
authors.initials: S
authors.lastname: Meza-Perez
authors.forename: Oscar
authors.initials: O
authors.lastname: Ramirez-Pliego
authors.forename: Maria A
authors.initials: MA
authors.lastname: Santana-Calderon
authors.forename: Pavel
authors.initials: P
authors.lastname: Espino-Solis
authors.forename: Lourdes
authors.initials: L
authors.lastname: Gutierrez-Xicotencatl
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Flores-Romo
authors.forename: Fernando R
authors.initials: FR
authors.lastname: Esquivel-Guadarrama
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001483:Base Sequence; D019013:CD40 Antigens / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012400:Rotavirus Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D012333:RNA, Messenger