title: Hydrogen sulfide promotes root organogenesis in Ipomoea batatas, Salix matsudana and Glycine max.
abstract: In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H(2)S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H(2)S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventitious roots in a dose-dependent manner. It was also verified that H(2)S or HS(-) rather than other sulfur-containing components derived from NaHS could be attributed to the stimulation of adventitious root formation. A rapid increase in endogenous H(2)S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HaHS. Further investigation showed that H(2)S-mediated root formation was alleviated by N-1-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H(2)S donor-dependent root organogenesis were observed in both excised willow (Salix matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H(2)S-induced adventitious root formation was likely mediated by IAA and NO, and that H(2)S acts upstream of IAA and NO signal transduction pathways.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Wei-Yan
authors.initials: WY
authors.lastname: Peng
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.forename: Dai-Fu
authors.initials: DF
authors.lastname: Ma
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Wei
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Hu
mesh_terms: D006862:Hydrogen Sulfide / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism; D027723:Ipomoea batatas / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development*; D032108:Salix / Q000502:physiology*; D036226:Seedlings / Q000254:growth & development; D015398:Signal Transduction; D013025:Glycine max / Q000502:physiology*; D013440:Sulfides / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; D013440:Sulfides; D009569:Nitric Oxide; C030737:indoleacetic acid; C025451:sodium bisulfide; D006862:Hydrogen Sulfide