A mixture of peptides and sugars derived from plant cell walls increases plant defense responses to stress and attenuates ageing-associated molecular changes in cultured skin cells.
Small peptides and aminoacid derivatives have been extensively studied for their effect of inducing plant defense responses, and thus increasing plant tolerance to a wide range of abiotic stresses. Similarly to plants, these compounds can activate different signaling pathways in mammalian skin cells as well, leading to the up-regulation of anti-aging specific genes. This suggests the existence of analogous defense response mechanisms, well conserved both in plants and animal cells. In this article, we describe the preparation of a new mixture of peptides and sugars derived from the chemical and enzymatic digestion of plant cell wall glycoproteins. We investigate the multiple roles of this product as potential "biostimulator" to protect plants from abiotic stresses, and also as potential cosmeceutical. In particular, the molecular effects of the peptide/sugar mixture of inducing plant defense responsive genes and protecting cultured skin cells from oxidative burst damages were deeply evaluated.
Apone
Fabio
F
Arterra Bioscience, via Brin 69, 80142 Napoli, Italy. fapone@arterrabio.it
Tito
Annalisa
A
Carola
Antonietta
A
Arciello
Stefania
S
Tortora
Assunta
A
Filippini
Lucio
L
Monoli
Irene
I
Cucchiara
Mirna
M
Gibertoni
Simone
S
Chrispeels
Maarten J
MJ
Colucci
Gabriella
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002241:Carbohydrates / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry* / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D020552:Comet Assay; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology; D013058:Mass Spectrometry; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology*; D013312:Stress, Physiological / Q000187:drug effects*; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects
D000975:Antioxidants; D029681:Arabidopsis Proteins; D002241:Carbohydrates; D010455:Peptides; D017382:Reactive Oxygen Species; D003094:Collagen; D020782:Matrix Metalloproteinases