Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation.
Cell-permeable chemical inhibitors have become invaluable reagents for the study of cellular regulation. In the present paper, using protein kinase inhibitors as the example, a set of criteria are described that should be met before any investigation using such compounds should be accepted for publication.
Cohen
Philip
P
MRC Protein Phosphorylation Unit, The Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK. p.cohen@dundee.ac.uk
D000818:Animals; D002460:Cell Line; D017408:Guidelines as Topic; D006801:Humans; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000502:physiology; D015262:Xenobiotics / Q000737:chemistry / Q000494:pharmacology*
D047428:Protein Kinase Inhibitors; D011506:Proteins; D015262:Xenobiotics; D011494:Protein Kinases