title: [Relationship between primary ocular adnexal mucosa-associated lymphoid tissue lymphoma and eye infection].
abstract: OBJECTIVE
  To study the role of pathogenic microorganisms commonly associated with chronic eye disease, including Chlamydia psittaci, Chlamydia trachomatis, Chlamydia pneumoniae, herpes simplex virus (HSV) type 1 and type 2, and adenovirus type 8 and type 19, in the development of primary ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma in Chinese patients.
  
  
  METHODS
  Sixty-eight archival cases of primary ocular adnexal lymphoproliferative lesions, including 38 cases of MALT lymphoma, 3 cases of non-MALT lymphoma and 27 cases of chronic inflammation, were enrolled into the study. DNA was extracted from the paraffin-embedded tissue samples. The presence of DNA of C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 were analyzed by multiplex touchdown enzyme time-release polymerase chain reaction (TETR-PCR).
  
  
  RESULTS
  All of the specimens yielded PCR products of over 100 base pairs and were thus suitable for TETR-PCR screening of infectious agents. The prevalence of DNA of C. psittaci, C. trachomatis and adenovirus type 19 were 0 in MALT lymphoma, non-MALT lymphoma and chronic inflammation. There were 2 cases positive for C. pneumoniae DNA, amongst the 38 cases of MALT lymphoma studied (5.3%, 2/38). HSV type 1, HSV type 2 and adenovirus type 8 DNA was found in each of the 3 patients with chronic inflammation.
  
  
  CONCLUSIONS
  The study indicates that C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 probably play little role in the pathogenesis of ocular adnexal MALT lymphoma in Chinese patients.
authors.affiliation: Department of Pathology, Basic Medical College, Capital Medical University, Beijing 100069, China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Zhang
authors.forename: Hong-gang
authors.initials: HG
authors.lastname: Liu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Zi-fen
authors.initials: ZF
authors.lastname: Gao
authors.forename: Xiao-ge
authors.initials: XG
authors.lastname: Zhou
authors.forename: Ha-si
authors.initials: HS
authors.lastname: Jin
authors.forename: Li-na
authors.initials: LN
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Gong
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D023521:Chlamydophila Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D015817:Eye Infections / Q000382:microbiology / Q000821:virology; D005134:Eye Neoplasms / Q000382:microbiology / Q000821:virology; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology / Q000821:virology; D009956:Psittacosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral