Primary breast carcinoma: association of mammographic calcifications with osteopontin expression.
OBJECTIVE
To evaluate the osteopontin (OPN) protein expression levels in breast carcinomas to determine if they correlate with mammographic appearances such as calcifications.


METHODS
This retrospective study was institutional review board approved. Informed consent was obtained from patients. Clinical history, histopathologic findings, mammographic features, and OPN expression as determined with immunohistochemistry results were evaluated in 141 women with breast cancer. The median age of patients was 53 years (range, 29-82 years). Mammographic features and clinicopathologic characteristics were correlated with tumor OPN expression. chi(2) And Fisher exact tests were used to evaluate the association of OPN expression with mammographic and clinicopathologic features.


RESULTS
Calcifications on mammograms (P = .012), spiculated margins of mass on mammograms (P = .02), "triple-negative" (ie, cancer that is estrogen receptor, progesterone receptor, and human epidermal growth factor receptor negative) phenotype (P = .02), and lymph node metastasis (P < .0001) were significantly associated with OPN status. In contrast to OPN-negative tumors, OPN-positive tumors were more likely to have spiculated margins (57.6% vs 9.2%), to be associated with calcifications (54.3% vs 30.6%), to be a triple-negative phenotype (26% vs 8.1%), and to have axillary lymph node metastasis (81.5% vs 38.8%). Most calcifications were of pleomorphic morphology (60.4% vs 11.8%, P = .046).


CONCLUSIONS
OPN could play a role in the formation of calcifications that often are associated with breast cancer.
Wang
Xiao
X
Department of Breast Surgery, Jinan Central Hospital, Medical College, Shandong University, 105 Jiefang Road, Jinan 250013, China. wangxiao@sdu.edu.cn
Chao
Lan
L
Ma
Guohui
G
Chen
Liansheng
L
Jin
Guangchao
G
Hua
Mei
M
Liu
Huiping
H
Ouyang
Aimei
A
Zhang
Xiulan
X
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D002114:Calcinosis / Q000000981:diagnostic imaging* / Q000378:metabolism*; D016009:Chi-Square Distribution; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008327:Mammography; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D053495:Osteopontin / Q000378:metabolism*; D010641:Phenotype; D012189:Retrospective Studies
D015415:Biomarkers; D053495:Osteopontin