Surface Plasmon Resonance Imaging sensor for cathepsin determination based on immobilized cystatin.
A specific SPRI sensor for cathepsin determination based on the interaction between immobilized cystatin and cathepsins has been developed. All cathepsins form the same calibration curve. The sensor dynamic response range is between 0.5 and 2.0 ng ml(-1) and the detection limit is equal to 0.1 ng ml(-1).
Gorodkiewicz
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Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, Bialystok, Poland. ewka@uwb.edu.pl
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002403:Cathepsins / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D002645:Chickens; D015891:Cystatins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D007938:Leukemia / Q000097:blood; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D015891:Cystatins; D055767:Immobilized Proteins; D002403:Cathepsins