Analytical protocol for identification of BMAA and DAB in biological samples.
Beta-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.
Spácil
Zdenek
Z
Department of Analytical Chemistry, Stockholm University, 10691 Stockholm, Sweden.
Eriksson
Johan
J
Jonasson
Sara
S
Rasmussen
Ulla
U
Ilag
Leopold L
LL
Bergman
Birgitta
B
D000599:Amino Acids, Diamino / Q000032:analysis* / Q000302:isolation & purification; D000613:Aminobutyrates / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D006801:Humans; D019636:Neurodegenerative Diseases / Q000378:metabolism; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000599:Amino Acids, Diamino; D000613:Aminobutyrates; D000087522:Cyanobacteria Toxins; C001824:beta-N-methylamino-L-alanine; C522314:2,3-diaminobutanoic acid