Addition of adipose-derived stem cells in cord blood cultures stimulates their pluripotent differentiation.
BACKGROUND
Adipose tissue is recognized as an important source of postnatal mesenchymal stem cells for generative medicine applications. Moreover, cord blood stem cells have been shown to contain pluripotent stem cells called unrestricted somatic stem cells (USSCs). However, this population is rare and cannot be generated from every cord blood sample. In this study, we have presented a new method of co-culture of adipose-derived stem cells (ADPCs) and cord blood stem cells that results in pluripotent differentiation.


METHODS
ADPCs were obtained from a piece of adipose tissue after treatment with 0.075% collagenase, which was subsequently inactivated with DMEM/10% FBS. The cellular pellet of centrifugation was plated at 5-7 x 10(6) cells/mL in T25 culture flasks in a low-glycose DMEM with 30% FCS. Cord blood stem cells were obtained by centrifugation following double-processing in the presence of 2% HES 200/0.5 and plated at 5-7 x 10(6) cells/mL in the same medium. To investigate the crucial role of ADPCs in pluripotent cord blood differentiation, we added a ADPCS as (1 x 10(4) cells/mL) to the cord blood cultures and analyzed the contribution of ADPCs using a microscope as well as with flow cytometry.


RESULTS
After only 3 days, adherent cells (USSC colonies) of fibroblastic morphology were detected in all co-cultured samples, whereas this was observed later or not at all in the non-co-cultured samples. The greater density of colonies in the co-coltured samples was another point. Hematopoietic CD45 cells were no longer detected after the first passage. Pluripotent stem cells were obtained from all co-cultured samples that contained stem cells positive for CD29, CD44, CD49e, CD90, CD105, CD51 Stro, and C-kit antibodies but negative for CD34, CD45, CD133, and glycophorin A.


CONCLUSIONS
Addition of ADPCs was crucial to generate pluripotent-derived stem cells from cord blood samples. This double culture may be a useful tool for a universal allogeneic stem cell source for tissue repair or regeneration.
Tsagias
N
N
3rd University Obstetrics and Gynaecology Clinic, Ippokration General Hospital, Medical School, Aristotle University Thessaloniki, Thessaloniki 54124, Greece.
Kouzi-Koliakos
K
K
Koliakos
I
I
Kostidou
E
E
Karagiannis
V
V
Daniilidis
A
A
Koliakos
G
G
D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology*; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods; D015497:HIV-1 / Q000302:isolation & purification; D015498:HIV-2 / Q000302:isolation & purification; D016174:Hepacivirus / Q000302:isolation & purification; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D010920:Placenta / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*; D014015:Tissue Banks / Q000592:standards
D006514:Hepatitis B Surface Antigens