DDIT3/CHOP and the sarcoma fusion oncoprotein FUS-DDIT3/TLS-CHOP bind cyclin-dependent kinase 2.
BACKGROUND
The DDIT3 gene encodes a transcription factor belonging to the CCAAT/enhancer binding protein (C/EBP) family. It is normally expressed at very low levels but is activated by cellular stress conditions and induces G1 arrest and, in some cell types, apoptosis. DDIT3 is found as a part of the fusion oncogene FUS-DDIT3 that is causal for the development of myxoid/round-cell liposarcomas (MLS/RCLS).


RESULTS
In the present study, we searched for putative interaction partners of DDIT3 and the oncogenic FUS-DDIT3 among G1 cyclins and cyclin-dependent kinases. We found that FUS-DDIT3 and the normal DDIT3 bind CDK2. In addition, CDK2 showed an increased affinity for cytoskeletal proteins in cells expressing FUS-DDIT3 and DDIT3.


CONCLUSIONS
We conclude that DDIT3 binds CDK2 and that many of the observed biological effects of DDIT3 may involve interaction with CDK2.
Bento
Christoffer
C
Lundberg Laboratory for Cancer Research, Department of Pathology, University of Gothenburg, Gothenburg, Sweden. christoffer.bento@llcr.med.gu.se
Andersson
Mattias K
MK
Aman
Pierre
P
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018208:Liposarcoma, Myxoid / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment; D051742:Transcription Factor CHOP / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C532377:FUS-DDIT3 fusion protein, human; D015514:Oncogene Proteins, Fusion; D051742:Transcription Factor CHOP; D051357:Cyclin-Dependent Kinase 2