Microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil.
A Gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated MNA2(T), was isolated from a hexachlorocyclohexane-contaminated soil in North India. Strain MNA2(T) showed 95 % 16S rRNA gene sequence similarity with Microbacterium halotolerans YIM 70130(T). Phylogenetic analysis of 16S rRNA gene sequences showed that strain MNA2(T) belonged to a clade represented by the genus Microbacterium of the family Microbacteriaceae. Strain MNA2(T) contained anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(16 : 0) and iso-C(15 : 0) as the predominant fatty acids and diphosphatidylglycerol, phosphatidylglycerol and two unknown polar lipids. The menaquinones were MK-12, MK-11, MK-13 and MK-10, in an approximate molar ratio of 45 : 40 : 13 : 3, respectively. The DNA G+C content was 65.3 mol%. The peptidoglycan was of the B type of cross-linkage with ornithine as the diagnostic diamino acid. The results of the phylogenetic, phenotypic and chemotaxonomic analysis indicate that strain MNA2(T) belongs to a previously unrecognized species of the genus Microbacterium, for which the name Microbacterium lindanitolerans sp. nov. is proposed. The type strain is MNA2(T) (=DSM 22422(T) =CCM 7585(T)).
Lal
Devi
D
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
Gupta
Sanjay Kumar
SK
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Lal
Rup
R
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D001556:Hexachlorocyclohexane