Deficiency of Bid protein reduces sepsis-induced apoptosis and inflammation, while improving septic survival.
Increased apoptotic cell death is believed to play a pathological role in patients with sepsis and experimental animals. Apoptosis can be induced by either a cell death receptor (extrinsic) or a mitochondrial (intrinsic) pathway. Bid, a proapoptotic member of the Bcl-2 family, is thought to mediate the cross talk between the extrinsic and intrinsic pathways of apoptosis; however, little is known about the action of Bid in the development of apoptosis and organ-specific tissue damage/cell death as seen in polymicrobial sepsis. Our results show that after the onset of sepsis, tBid (the active form of Bid) is significantly increased in mitochondrial fractions of the thymus, spleen, Peyer patches, and liver, and that Fas or FasL deficiency blocks Bid activation in various tissues after septic challenge. Increased Bid activation is correlated with increased active caspase-3, caspase-9, and apoptosis during sepsis. Bid-deficient mice exhibit significantly reduced apoptosis in the thymus, spleen, and Peyer patches compared with background mice after sepsis. Furthermore, Bid-deficient mice had significantly reduced systemic and local inflammatory cytokine levels and improved survival after sepsis. These data support not only the contribution of Bid to sepsis-induced apoptosis and the onset of septic morbidity/mortality, but also the existence of a bridge between extrinsic apoptotic signals, e.g., FasL:Fas, TNF:TNFR, and so on, and the intrinsic mitochondrial pathway via Bid-tBid activation during sepsis.
Chung
Chun-Shiang
CS
Division of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, RI 02903, USA.
Venet
Fabienne
F
Chen
Yaping
Y
Jones
Leslie N
LN
Wilson
Douglas C
DC
Ayala
Carol A
CA
Ayala
Alfred
A
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000172:deficiency* / Q000502:physiology; D053453:Caspase 9 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D053222:Fas Ligand Protein / Q000502:physiology; D007249:Inflammation / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D021381:Protein Transport; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology; D018805:Sepsis / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology; D013154:Spleen / Q000276:immunology
D051023:BH3 Interacting Domain Death Agonist Protein; D016207:Cytokines; D053222:Fas Ligand Protein; D018124:Receptors, Tumor Necrosis Factor; D000410:Alanine Transaminase; D053453:Caspase 9