Effects of sesame oil in the model of adjuvant arthritis.
OBJECTIVE
The goal of this study was to evaluate the effect of sesame oil on functional damage induced by adjuvant arthritis (AA) and on changes of selected biochemical parameters reflecting oxidative tissue injury.


METHODS
Mycobacterium butyricum in incomplete Freund's adjuvans was intradermally administered to Lewis male rats. Hind paw edema and endothelium-dependent relaxation of the aorta were determined on day 28. Further, plasmatic levels of TBARS, gamma-glutamyltransferase (GGT) activity in the joint and spleen tissues, level of protein carbonyls and total antioxidant capacity (TAC) in plasma, as well as activity of the lysosomal enzyme N-acetyl-glucosaminidase (NAGA) in serum were assessed. The effect of sesame oil (SO, 1ml/kg, daily oral administration) was evaluated on day 28.


RESULTS
The beneficial effect of sesame oil on markers of oxidative stress accompanying AA was demonstrated by decrease of plasma TBARS and decrease of GGT activity in the joint and spleen tissues. Level of protein carbonyls, TAC in plasma and activity of NAGA in serum and in the kidney were improved, yet not significantly. In the hind paw edema the maximal increase was found on day 28 of AA, and in the same time we observed a significant decrease of aortic endothelium-dependent relaxation. Administration of SO resulted in mild, non-significant decrease of hind paw swelling and in significantly increased acetylcholine-evoked relaxation.


CONCLUSIONS
We conclude that SO has beneficial effects on oxidative stress induced biochemical changes occurring in AA, moreover it improves endothelium-dependent relaxation of the aorta and tends to decrease hind paw edema.
Sotnikova
Ruzena
R
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia. exfarosa@savba.sk
Ponist
Silvester
S
Navarova
Jana
J
Mihalova
Danica
D
Tomekova
Veronika
V
Strosova
Miriam
M
Bauerova
Katarina
K
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001011:Aorta / Q000503:physiopathology; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000178:diet therapy* / Q000503:physiopathology; D004487:Edema / Q000139:chemically induced / Q000178:diet therapy / Q000503:physiopathology; D005620:Freund's Adjuvant; D006596:Hexosaminidases / Q000378:metabolism; D007155:Immunologic Factors / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D009161:Mycobacterium; D018384:Oxidative Stress / Q000502:physiology; D050050:Protein Carbonylation / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D012715:Sesame Oil / Q000008:administration & dosage / Q000627:therapeutic use*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000975:Antioxidants; D007155:Immunologic Factors; D017392:Thiobarbituric Acid Reactive Substances; D012715:Sesame Oil; D005620:Freund's Adjuvant; D005723:gamma-Glutamyltransferase; D006596:Hexosaminidases