title: Effect of ultraviolet radiation on acetylcholinesterase activity in freshwater copepods.
abstract: We analyzed the effects of UV radiation (UVR) effects on acetylcholinesterase (AChE) activity in two calanoid copepods, Boeckella gibbosa and Parabroteas sarsi that inhabit Patagonian shallow lakes. We studied the effect of experimental UVR (UV-B and UV-A) exposure on AChE activity in relation to basal antioxidant capacities of both copepods. Our experiments showed that UVR can effectively depress AChE activity, although with differences between species. In both copepods AChE was affected by UV-B, whereas UV-A only affected AChE in B. gibbosa. Both copepods also differed in body elemental composition (C:N:P), photoprotecting compound content (carotenoids and mycosporine-like amino acids) and enzymatic antioxidant capacity (glutathione S-transferase [GST]). Our results suggest that when exposed to UVR, AChE activity would depend more on the antioxidant capacity (GST) and P availability for enzyme synthesis than on the photoprotective compounds.
authors.affiliation: Laboratorio de Limnología, INIBIOMA, CONICET-UNComahue, Bariloche, Argentina. ssouza@crub.uncoma.edu.ar
authors.forename: María Sol
authors.initials: MS
authors.lastname: Souza
authors.forename: Esteban
authors.initials: E
authors.lastname: Balseiro
authors.forename: Cecilia
authors.initials: C
authors.lastname: Laspoumaderes
authors.forename: Beatriz
authors.initials: B
authors.lastname: Modenutti
mesh_terms: D000110:Acetylcholinesterase / Q000528:radiation effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D033342:Copepoda; D005618:Fresh Water; D005982:Glutathione Transferase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D005982:Glutathione Transferase; D000110:Acetylcholinesterase