title: Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes.
abstract: This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.
authors.affiliation: Office of Research and Development, National Risk Management Laboratory (MS387), United States Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
authors.forename: Hodon
authors.initials: H
authors.lastname: Ryu
authors.forename: Brooke
authors.initials: B
authors.lastname: Mayer
authors.forename: Morteza
authors.initials: M
authors.lastname: Abbaszadegan
mesh_terms: D000818:Animals; D003458:Cryptosporidium; D016133:Polymerase Chain Reaction / Q000379:methods*; D014780:Viruses; D014867:Water / Q000469:parasitology*; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D014867:Water