title: Resorption and elimination kinetics of benzoic acid during chemical necrectomy in deep burns.
abstract: OBJECTIVE
  Chemical necrectomy is an alternative to the surgical or sharp necrectomy for the removal of necrotic parts of the skin in the treatment of deep burns. The aim of our work was to monitor the dynamics of resorption and elimination of benzoic acid applied to the burnt skin.
  
  
  METHODS
  The set consisted of 10 patients (9 men; 1 woman) aged 25-57 years with IIb-III-degree skin burns. 40% benzoic acid in white petrolatum was applied to the burnt area to the extent of 3-5% of TBSA (total body surface area) for a period of 48 hours. The concentrations of benzoic acid, hippuric acid, and glycine in the serum was monitored at the 10th, 20th, 60th, 120th, 240 th and 360 th minute thereafter and further at the 12th, 24th, 48th, and 72nd hour; the excretion of hippuric acid in urine was monitored in six 12-hour intervals.
  
  
  RESULTS
  The highest concentration of benzoic acid in the serum was detected in the 60th minute sample (0.094+/-0.074 mmol/L) and of hippuric acid in the 120th minute sample (0.234+/-0.088 mmol/L) from the application of benzoic acid to the burnt skin. In the period between the 6th and 48th hour, the average concentration of benzoic acid in the serum ranged between 0.042 and 0.03 mmol/L. In this period there was also a significant decrease in serum glycine concentration (p<0.05). During the 48-hour application of benzoic acid to the burnt skin, 46.0-145 mmol of hippuric acid was excreted in urine.
  
  
  CONCLUSIONS
  Chemical necrectomy with the use of 40% benzoic acid led only to a moderate increase of its concentration in the serum. After its resorption from the wound area it is transformed to hippuric acid, which is promptly excreted in urine.
authors.affiliation: Department of Clinical Biochemistry and Hematology, University Hospital Brno, Medical Faculty, Masaryk University, Brno, Czech Republic. mdast@fnbrno.cz
authors.forename: Milan
authors.initials: M
authors.lastname: Dastych
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Jelínková
authors.forename: Alena
authors.initials: A
authors.lastname: Mikusková
authors.forename: Pavel
authors.initials: P
authors.lastname: Brychta
authors.forename: Miroslava
authors.initials: M
authors.lastname: Benovská
mesh_terms: D000328:Adult; D019817:Benzoic Acid / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D002056:Burns / Q000188:drug therapy* / Q000473:pathology*; D005260:Female; D005998:Glycine / Q000097:blood; D006626:Hippurates / Q000097:blood / Q000652:urine; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009336:Necrosis / Q000473:pathology*; D009824:Ointments / Q000008:administration & dosage
chemical_list: D006626:Hippurates; D009824:Ointments; D019817:Benzoic Acid; C030514:hippuric acid; D005998:Glycine