title: Multilineage differentiation potential of fibroblast-like stromal cells derived from human skin.
abstract: Adult stem cells that reside in adult tissues have been deemed to possess great potential for clinical application because of their capabilities of self-renewal and differentiation. However, the limitations such as infection risks and low isolation rate make the search for appropriate source to be continued. Here, we demonstrate isolation of progenitors from human foreskin tissue samples, which have fibroblast-like morphology and could be easily propagated for more than 50 passages. These foreskin-derived fibroblast-like stromal cells (FDSCs) expressed CD90, CD105, CD166, CD73, SH3, and SH4, which is similar to the immunophenotypes of human bone marrow-derived mesenchymal stem cells. In comparison with Hs68, the human fibroblast cell line, FDSCs are positive for CD105 and absent for CD54 expression. Further, FDSCs could be induced to differentiate into osteocytes, adipocytes, neural cells, smooth muscle cells, Schwann-like cells, and hepatocyte-like cells. Interestingly, when cultured in Dulbecco's modified Eagle's medium/F12 medium, FDSCs can form spheres with increased expression levels of fibronectin and vimentin. In conclusion, foreskin can serve as a valuable source of multipotent cells with the capabilities for endodermal, mesodermal, and ectodermal cells. Coupled with the advantages of their easy access, isolation, and propagation, these foreskin-derived stromal cells might be of potential use in future studies in stem cell differentiation and therapeutic application.
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259, Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan 221, Taiwan. hihuang@mail.cgu.edu.tw
authors.forename: Hsing-I
authors.initials: HI
authors.lastname: Huang
authors.forename: Shao-Kuan
authors.initials: SK
authors.lastname: Chen
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chien
authors.forename: Hsiao-Tung
authors.initials: HT
authors.lastname: Liu
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Chan
mesh_terms: D017667:Adipocytes / Q000166:cytology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D010011:Osteocytes / Q000166:cytology; D010012:Osteogenesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000166:cytology; D012867:Skin / Q000166:cytology*; D017154:Stromal Cells
chemical_list: D015415:Biomarkers