FCS-based sensing for the detection of ochratoxin and neomycin in food.
In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.
Varriale
Antonio
A
Laboratory for Molecular Sensing, IBP-CNR, Naples, Italy.
Staiano
Maria
M
Iozzino
Luisa
L
Severino
Lorella
L
Anastasio
Aniello
A
Cortesi
Maria Luisa
ML
D'Auria
Sabato
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D005455:Fluorescent Antibody Technique; D005506:Food Contamination / Q000032:analysis*; D009355:Neomycin / Q000032:analysis* / Q000276:immunology; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000906:Antibodies; D009793:Ochratoxins; C025589:ochratoxin A; D009355:Neomycin