GonadSAGE: a comprehensive SAGE database for transcript discovery on male embryonic gonad development.
Serial analysis of gene expression (SAGE) provides an alternative, with additional advantages, to microarray gene expression studies. GonadSAGE is the first publicly available web-based SAGE database on male gonad development that covers six male mouse embryonic gonad stages, including E10.5, E11.5, E12.5, E13.5, E15.5 and E17.5. The sequence coverage of each SAGE library is beyond 150K, 'which is the most extensive sequence-based male gonadal transcriptome to date'. An interactive web interface with customizable parameters is provided for analyzing male gonad transcriptome information. Furthermore, the data can be visualized and analyzed with the other genomic features in the UCSC genome browser. It represents an integrated platform that leads to a better understanding of male gonad development, and allows discovery of related novel targets and regulatory pathways.
Lee
Tin-Lap
TL
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. leetl@mail.nih.gov
Li
Yunmin
Y
Cheung
Hoi-Hung
HH
Claus
Janek
J
Singh
Sumeeta
S
Sastry
Chandan
C
Rennert
Owen M
OM
Lau
Yun-Fai Chris
YF
Chan
Wai-Yee
WY
D000818:Animals; D030541:Databases, Genetic; D047108:Embryonic Development / Q000235:genetics; D015870:Gene Expression; D023281:Genomics / Q000379:methods*; D006066:Gonads / Q000254:growth & development*; D008297:Male; D051379:Mice; D012984:Software