title: Screening and characterization of microorganisms with FR901379 acylase activity.
abstract: A simple and rapid screen was performed for microorganisms producing cyclic lipopeptide acylase, an enzyme that deacylates the acyl side chain of FR901379 to yield a cyclic peptide, FR179642, which is an important intermediate in producing micafungin. Among approximately 3800 newly isolated strains from soil samples, 5 microorganisms expressing high acylase activity were selected and classified, 3 as Streptomyces spp. and 2 as filamentous fungi. This is the first time that these strains have been identified as cyclic lipopeptide acylase producers. Both fungi and streptomycetes showed differing optimum pH and temperature profiles for acylase activity.
authors.affiliation: Fermentation and Biotechnology Laboratories, Astellas Pharma Inc., Kiyosu-City, Aichi, Japan. satoshi-ueda@jp.astellas.com
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ueda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Oohata
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsuboi
authors.forename: Michio
authors.initials: M
authors.lastname: Yamashita
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hino
authors.forename: Masato
authors.initials: M
authors.lastname: Yamada
authors.forename: Seiji
authors.initials: S
authors.lastname: Hashimoto
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000935:Antifungal Agents; D001431:Bacteriological Techniques; D054714:Echinocandins; D005656:Fungal Proteins; D005658:Fungi / Q000201:enzymology*; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000378:metabolism*; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; C110368:FR 901379; D005656:Fungal Proteins; D010456:Peptides, Cyclic; C007572:echinocandin B; D000581:Amidohydrolases; C019665:amidase