title: [Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2].
abstract: OBJECTIVE
  Immortalized cell lines of spinocerebellar ataxia type 2 (SCA2) with Parkinson disease symptoms were established in order to provide experimental material for future study.
  
  
  METHODS
  The immortalized cell lines were constructed by using Epstein Barr virus and cyclosporine A. Microsatellite markers were detected to see whether there is any change between the cell lines and the original blood samples, and the genetic stability of the cell lines were evaluated.
  
  
  RESULTS
  Twenty-five immortalized cell lines were established successfully from the family and the microsatellite markers were unchanged.
  
  
  CONCLUSIONS
  The karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, 650118 P.R. China.
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Lin
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.forename: Chang-jun
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Yu-fen
authors.initials: YF
authors.lastname: Tao
authors.forename: Wen
authors.initials: W
authors.lastname: Yi
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.forename: Jia-you
authors.initials: JY
authors.lastname: Chu
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D020754:Spinocerebellar Ataxias / Q000235:genetics*; D055815:Young Adult