[Establishment of endometriosis diagnostic model using plasma protein profiling].
OBJECTIVE
To establish the diagnostic model for endometriosis by screening the plasma biomarkers of endometriosis using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)coupled with bioinformatics.


METHODS
Plasma samples from 36 patients with endometriosis (endometriosis group) matched with 35 patients with infertility or benign ovarian tumors (control group) before laparoscopy were collected at Peking Union Medical College Hospital from January to October 2007. Plasma protein profiling were detected using SELDI-TOF-MS and protein chip and peak intensities were compared between the two groups. Biomarker Discovery Software was used for data analysis and model was build by classification and regression tree software (CART), sensitivity and specificity of the diagnostic model were verified.


RESULTS
There were 14 protein peaks abnormally expressed in endometriosis group compared with those of control group (P < 0.01). The diagnostic model composed of three protein peaks with the molecular weight of 3,956,000, 11,710,000 and 6,986,000 showed a sensitivity of 92% and specificity of 83%. In the blind test the model showed a sensitivity of 88% and specificity of 80%.


CONCLUSIONS
SELDI-TOF-MS is a new approach for screening markers of endometriosis. Its clinical value deserves further investigation.
Liu
Hai-yuan
HY
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zheng
Yan-hua
YH
Zhang
Jian-zhong
JZ
Leng
Jin-hua
JH
Sun
Da-wei
DW
Liu
Zhu-feng
ZF
Zhu
Lan
L
Lang
Jing-he
JH
D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D004715:Endometriosis / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008954:Models, Biological; D008970:Molecular Weight; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015415:Biomarkers; D001798:Blood Proteins