title: Oral keratinocyte responses to nickel-based dental casting alloys in vitro.
abstract: Adverse reactions of oral mucosa to nickel-based dental casting alloys are probably due to corrosion metal ion release. We exposed H400 oral keratinocytes to two Ni-based dental alloys (Matchmate and Dsign10) as well as NiCl( 2) (1-40 microg/mL Ni(2+)). Alloy derived Ni(2+) media concentrations were determined. Direct culture on both alloys resulted in inhibited growth with a greater effect observed for Dsign10 (higher ion release). Indirect exposure of cells to conditioned media from Dsign10 negatively affected cell numbers (approximately 64% of control by 6 days) and morphology while Matchmate-derived media did not. Exposure to increasing NiCl(2) negatively affected cell growth and morphology, and the Granulocyte-macrophage colony-stimulating factor (GM-CSF) transcript was significantly up-regulated in cells following direct and indirect exposure to Dsign10. NiCl(2) exposure up-regulated all cytokine transcripts at 1 day. At day 6, IL-1beta and IL-8 transcripts were suppressed while GM-CSF and IL-11 increased with Ni(2+) dose. Accumulation of Ni(2+) ions from alloys in oral tissues may affect keratinocyte viability and chronic inflammation.
authors.affiliation: School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Wylie
authors.forename: A J
authors.initials: AJ
authors.lastname: Davenport
authors.forename: P R
authors.initials: PR
authors.lastname: Cooper
authors.forename: R M
authors.initials: RM
authors.lastname: Shelton
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003722:Dental Alloys / Q000009:adverse effects* / Q000378:metabolism; D003735:Dental Casting Technique; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology*; D009532:Nickel / Q000009:adverse effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D003722:Dental Alloys; C022838:nickel chloride; D009532:Nickel