Proteomic analysis of outer membrane proteins of Edwardsiella tarda.
OBJECTIVE
The purpose of this study was to identify outer membrane proteins (OMPs) of Edwardsiella tarda.


RESULTS
The OMPs from a virulent strain of E. tarda (ET-7) was extracted using lauroyl sarcosine method. The OMPs were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and protein spots were identified using matrix assisted laser desorption/ionization-time-of-flight mass spectrometry. A total of 21 proteins were identified from 24 protein spots observed on the 2D-PAGE gel. These proteins were identified as GroEL, antigenic proteins, ABC transporters, elongation factors, OmpA, PTSINtr with GAF domain, catalase C, glycolytic enzymes, DnaJ, transcriptional regulator, proteins mraZ and ccdA. Subcellular localizations, beta-barrel OMPs and lipoproteins of identified proteins were predicted using PSORTb, PRED-TMBB and LipoP1.0 programme.


CONCLUSIONS
Identification, localization and possible functions of OMPs of E. tarda were studied.


CONCLUSIONS
These proteins could be used for development of novel drug targets, diagnostics or vaccine against edwardsiellosis.
Kumar
G
G
National Bureau of Fish Genetic Resources (ICAR), Lucknow, India.
Sharma
P
P
Rathore
G
G
Bisht
D
D
Sengupta
U
U
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D020609:Edwardsiella tarda / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001425:Bacterial Outer Membrane Proteins; D020543:Proteome