title: Chlamydophila pneumoniae infection leads to smooth muscle cell proliferation and thickening in the coronary artery without contributions from a host immune response.
abstract: Chlamydophila pneumonia (C. pneumonia) infection has been associated with the progression of atherosclerosis. It remains unclear, however, whether C. pneumoniae in the absence of an immune response can alone initiate atherogenic events within a complex vessel environment. Left anterior descending coronary arteries isolated from porcine hearts were dissected and placed in culture medium for 72 hours before infection with C. pneumoniae. C. pneumoniae replicated within the arterial wall for the duration of the experiment (up to 10 days). A significant increase in chlamydial-HSP60 protein expression from day 2 to 10 post-infection (pi) indicated the presence of metabolically active C. pneumonia within infected vessels. Significant arterial thickening in infected coronary segments was observed by a considerable decrease in the ratio of lumen to total vessel area (48 +/- 3% at day 4 pi versus 23 +/- 3% at day 10 pi) and a significant increase in the ratio of media to luminal area (113 +/- 16% at day 4 pi versus 365 +/- 65% at day 10 pi). Structural changes were accompanied by an up-regulation of host HSP60 and proliferating cell nuclear antigen expression levels. Immunohistochemical staining confirmed proliferating cell nuclear antigen expression to be primarily localized within smooth muscle cells of the medial area. These results demonstrate that C. pneumoniae infection can stimulate arterial thickening in a complex vessel environment without the presence of a host immune response and further supports the involvement of HSP60 in this action.
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, Canada R2H 2A6.
authors.forename: Justin F
authors.initials: JF
authors.lastname: Deniset
authors.forename: Paul K M
authors.initials: PK
authors.lastname: Cheung
authors.forename: Elena
authors.initials: E
authors.lastname: Dibrov
authors.forename: Kaitlin
authors.initials: K
authors.lastname: Lee
authors.forename: Sarah
authors.initials: S
authors.lastname: Steigerwald
authors.forename: Grant N
authors.initials: GN
authors.lastname: Pierce
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D023521:Chlamydophila Infections / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology / Q000662:veterinary; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000502:physiology*; D003331:Coronary Vessels / Q000276:immunology / Q000473:pathology*; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D006801:Humans; D055633:Immune System Phenomena / Q000502:physiology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology / Q000503:physiopathology; D032389:Myocytes, Smooth Muscle / Q000276:immunology / Q000473:pathology* / Q000502:physiology; D009924:Organ Culture Techniques; D009929:Organ Size; D013552:Swine