Developmental iodine deficiency and hypothyroidism impair neural development, up-regulate caveolin-1 and down-regulate synaptophysin in rat hippocampus.
Developmental iodine deficiency leads to inadequate thyroid hormone, which damages the hippocampus. In the present study, we implicate hippocampal caveolin-1 and synaptophysin in developmental iodine deficiency and hypothyroidism. Two developmental rat models were established: pregnant rats were administered either an iodine-deficient diet or propylthiouracil (PTU)-adulterated (5 p.p.m. or 15 p.p.m.) drinking water from gestational day 6 until postnatal day (PN) 28. Nissl staining and the levels of caveolin-1 and synaptophysin in several hippocampal subregions were assessed on PN14, PN21, PN28 and PN42. The results obtained show that surviving cells in the iodine-deficient and PTU-treated rats were lower than in controls. Up-regulation of caveolin-1 and down-regulation of synaptophysin were observed in the iodine-deficient and PTU-treated rats. Our findings implicate decreases in the number of surviving cells and alterations in the levels of caveolin-1 and synaptophysin in the impairments in neural development induced by developmental iodine deficiency and hypothyroidism.
Gong
J
J
Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, China.
Dong
J
J
Wang
Y
Y
Xu
H
H
Wei
W
W
Zhong
J
J
Liu
W
W
Xi
Q
Q
Chen
J
J
D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D002452:Cell Count; D002470:Cell Survival; D004032:Diet; D004195:Disease Models, Animal; D005260:Female; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007455:Iodine / Q000172:deficiency*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011441:Propylthiouracil; D017966:Pyramidal Cells / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016708:Synaptophysin / Q000378:metabolism*; D013997:Time Factors
C495537:Cav1 protein, rat; D051242:Caveolin 1; D016708:Synaptophysin; D011441:Propylthiouracil; D007455:Iodine