title: Vascular endothelial growth factor concentrations from platelets correlate with tumor angiogenesis and grading in a spontaneous canine non-Hodgkin lymphoma model.
abstract: Published data strongly suggest that tumor progression and malignancy are associated with increased angiogenesis. However, no data have been published concerning the relationship between microvascular density (MVD), tumor cytosol, and blood vascular endothelial growth factor (VEGF) concentrations in canine non-Hodgkin lymphoma (C-NHL), a neoplasm that shares biological and clinical characteristics with human NHL. We have evaluated MVD and tumor cytosol, serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) VEGF concentration in a series of 63 B-cells C-NHL by means of immunohistochemistry and enzyme-linked immuno-sorbent assay (ELISA) detection of VEGF. We found that MVD, VEGF from cytosol, and VEGF from P-APR are significantly correlated (p ranging from 0.001 to 0.003) and that these parameters paralleled with the malignancy degree of NHL. Accordingly, spontaneous C-NHL seems to be an interesting model to study the role of angiogenesis as interspecies pathway of tumor malignancy and we suggest that VEGF from P-APR might be a novel useful circulating bio-marker of tumor angiogenesis.
authors.affiliation: Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Zizzo
authors.forename: Rosa
authors.initials: R
authors.lastname: Patruno
authors.forename: Francesco Alfredo
authors.initials: FA
authors.lastname: Zito
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Summa
authors.forename: Antonella
authors.initials: A
authors.lastname: Tinelli
authors.forename: Sante
authors.initials: S
authors.lastname: Troilo
authors.forename: Andrea
authors.initials: A
authors.lastname: Misino
authors.forename: Eustachio
authors.initials: E
authors.lastname: Ruggieri
authors.forename: Veronica
authors.initials: V
authors.lastname: Goffredo
authors.forename: Cosmo Damiano
authors.initials: CD
authors.lastname: Gadaleta
authors.forename: Girolamo
authors.initials: G
authors.lastname: Ranieri
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000662:veterinary*; D055806:Microvessels / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000662:veterinary*; D053657:Platelet-Rich Plasma / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood* / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A