title: Analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine by liquid chromatography-tandem mass spectrometry.
abstract: The ability to non-invasively monitor DNA oxidation and its repair has significant utility in large-scale, population-based studies. Such studies could include assessments of the efficacy of antioxidant intervention strategies, pathological roles of DNA oxidation in various disease states and population or inter-individual differences in antioxidant defence and DNA repair. The analysis of urine, or indeed any extracellular matrix, for 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), using chromatographic or immunoassay procedures, is by far the most popular method to non-invasively assess oxidative insult to the genome. The actual biological significance of the presence of extracellular 8-oxodG is still a subject for debate however. Studies are slowly ruling out confounding factors such as diet and cell turnover, which would leave endogenous processes, notably repair, as the sole source of extracellular 8-oxodG. The method described herein exploits the non-invasive properties of urine sampling, coupled with efficient extraction of 8-oxodG by a validated solid-phase extraction procedure. Subsequent analysis by liquid chromatography-tandem mass spectrometry has the advantages of sensitivity, internal standardisation and robust peak identification.
authors.affiliation: Radiation & Oxidative Stress Group, Department of Cancer Studies, University of Leicester, Leicester, UK.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Evans
authors.forename: Rajinder
authors.initials: R
authors.lastname: Singh
authors.forename: Vilas
authors.initials: V
authors.lastname: Mistry
authors.forename: Peter B
authors.initials: PB
authors.lastname: Farmer
authors.forename: Marcus S
authors.initials: MS
authors.lastname: Cooke
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003404:Creatinine / Q000652:urine; D004247:DNA / Q000737:chemistry / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003404:Creatinine; D003849:Deoxyguanosine