title: Immunomodulatory activity of the lignan 7-hydroxymatairesinol potassium acetate (HMR/lignan) extracted from the heartwood of Norway spruce (Picea abies).
abstract: The pharmacological profile of the lignan 7-hydroxymatairesinol (HMR/lignan, HMR) includes chemopreventive effects, antioxidant properties, and mild proestrogenic activity. The present study was devised to investigate the effects of HMR on THP-1 cells, an established model of human monocytes, and on human polymorphonuclear leukocytes (PMNs). In THP-1 cells, HMR concentration-dependently reduced LPS-stimulated tumor necrosis factor (TNF)-alpha secretion in the supernatant. HMR at low, sub-muM concentrations also reduced TNF-alpha mRNA, which was however enhanced by supra-muM concentrations of HMR. In human PMNs, HMR concentration-dependently reduced ROS production induced by either N-formyl-Met-Leu-Phe, phorbol myristate acetate or angiotensin II, as well as interleukin-8 production induced by either N-formyl-Met-Leu-Phe or angiotensin II. Results indicate that HMR is an effective inhibitor of both monocytic THP-1 cells and of human PMNs and warrant further studies to assess their relevance for the prevention and treatment of several conditions characterized by chronic systemic inflammation.
authors.affiliation: Department of Clinical Medicine, University of Insubria, Varese, Italy. marco.cosentino@uninsubria.it
authors.forename: Marco
authors.initials: M
authors.lastname: Cosentino
authors.forename: Franca
authors.initials: F
authors.lastname: Marino
authors.forename: Ramòna Consuélo
authors.initials: RC
authors.lastname: Maio
authors.forename: Marco Gioacchino
authors.initials: MG
authors.lastname: Delle Canne
authors.forename: Marcello
authors.initials: M
authors.lastname: Luzzani
authors.forename: Silvano
authors.initials: S
authors.lastname: Paracchini
authors.forename: Sergio
authors.initials: S
authors.lastname: Lecchini
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D007155:Immunologic Factors; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis; D017705:Lignans / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D028222:Picea / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014934:Wood / Q000737:chemistry
chemical_list: D007155:Immunologic Factors; D016209:Interleukin-8; D017705:Lignans; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C419306:hydroxymatairesinol; D000804:Angiotensin II; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012313:RNA