Terminal deoxynucleotidyl transferase is required for an optimal response to the polysaccharide α-1,3 dextran.
An understanding of Ab responses to polysaccharides associated with pathogenic microorganisms is of importance for improving vaccine design, especially in neonates that respond poorly to these types of Ags. In this study, we have investigated the role of the lymphoid-specific enzyme TdT in generating B cell clones responsive to alpha-1,3 dextran (DEX). TdT is a DNA polymerase that plays a major role in generating diversity of lymphocyte AgRs during V(D)J recombination. In this study, we show that the DEX-specific Ab response is lower, and the dominant DEX-specific J558 idiotype (Id) is not detected in TdT(-/-) mice when compared with wild-type (WT) BALB/c mice. Nucleotide sequencing of H chain CDR3s of DEX-specific plasmablasts, sorted postimmunization, showed that TdT(-/-) mice generate a lower frequency of the predominant adult molecularly determined clone J558. Complementation of TdT expression in TdT(-/-) mice by early forced expression of the short splice variant of TdT-restored WT proportions of J558 Id+ clones and also abrogated the development of the minor M104E Id+ clones. J558 Id V(D)J rearrangements are detected as early as 7 d after birth in IgM-negative B cell precursors in the liver and spleen of WT and TdT-transgenic mice but not in TdT(-/-) mice. These data show that TdT is essential for the generation of the predominant higher-affinity DEX-responsive J558 clone.
Mahmoud
Tamer I
TI
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Kearney
John F
JF
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D004253:DNA Nucleotidylexotransferase / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D003911:Dextrans / Q000276:immunology*; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D056724:Immunity, Humoral; D007133:Immunoglobulin Joining Region / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D011134:Polysaccharides / Q000276:immunology
D003911:Dextrans; D007133:Immunoglobulin Joining Region; D011134:Polysaccharides; C040834:alpha-1,3-dextran; D004253:DNA Nucleotidylexotransferase