title: Asynchronous RAG-1 expression during B lymphopoiesis.
abstract: Changes in cell surface markers and patterns of gene expression are commonly used to construct sequences of events in hematopoiesis. However, the order may not be as rigid as once thought and it is unclear which changes represent the best milestones of differentiation. We developed a fate-mapping model where cells with a history of RAG-1 expression are permanently marked by red fluorescence. This approach is valuable for appreciating lymphoid-lineage relationships without need for irradiation and transplantation. Hematopoietic stem cells (HSC) as well as myeloid and dendritic cell progenitors were unlabeled. Also as expected, most previously identified RAG-1(+) early lymphoid progenitors in bone marrow and all lymphoid-affiliated cells were marked. Of particular interest, there was heterogeneity among canonical common lymphoid progenitors (CLP) in bone marrow. Labeled CLP expressed slightly higher levels of IL-7Ralpha, displayed somewhat less c-Kit, and generated CD19(+) lymphocytes faster than the unlabeled CLP. Furthermore, CLP with a history of RAG-1 expression were much less likely to generate dendritic and NK cells. The RAG-1-marked CLP were lineage stable even when exposed to LPS, while unlabeled CLP were redirected to become dendritic cells in response to this TLR4 ligand. These findings indicate that essential events in B lymphopoiesis are not tightly synchronized. Some progenitors with increased probability of becoming lymphocytes express RAG-1 while still part of the lineage marker-negative Sca-1(+)c-Kit(high) (LSK) fraction. Other progenitors first activate this locus after c-Kit levels have diminished and cell surface IL-7 receptors are detectable.
authors.affiliation: Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Welner
authors.forename: Brandt L
authors.initials: BL
authors.lastname: Esplin
authors.forename: Karla P
authors.initials: KP
authors.lastname: Garrett
authors.forename: Rosana
authors.initials: R
authors.lastname: Pelayo
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Hans Jörg
authors.initials: HJ
authors.lastname: Fehling
authors.forename: Paul W
authors.initials: PW
authors.lastname: Kincade
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000096:biosynthesis / Q000235:genetics; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D055879:Gene Knock-In Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D038041:Lymphopoiesis / Q000235:genetics / Q000276:immunology*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D000950:Antigens, Ly; D018398:Homeodomain Proteins; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; C064658:RAG-1 protein; D019009:Proto-Oncogene Proteins c-kit