title: Fabrication of cell pattern on poly(dimethylsiloxane) by vacuum ultraviolet lithography.
abstract: Cell patterning on substrates has played a significant role in the study of basic biology, cell-based biosensor and tissue engineering. In this report, a cell pattern was prepared on poly(dimethylsiloxane) (PDMS) substrate by vacuum ultraviolet (VUV) lithography. After immobilizing allyl-polyethylene glycol (APEG) onto PDMS, a chemical heterogeneous patterned surface was fabricated by VUV (Xe(2) excimer: 172nm) lithography with copper mesh as a photomask. The UV exposed domains can promote L929 cell adhesion and growth. However, non-exposed regions resist cell attachment because of the repelling property of PEG. Therefore, cell pattern could be achieved without pre-adsorption of cell adhesive species before cell culture.
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Gan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Zhongkui
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D014343:Trypan Blue / Q000737:chemistry / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D004129:Dimethylpolysiloxanes; D011092:Polyethylene Glycols; D014343:Trypan Blue