Determination of equilibrium association constants of ligand-DNA complexes by electrospray mass spectrometry.
Electrospray mass spectrometry can be used to detect ligand-DNA noncovalent complexes formed in solution. This chapter describes how to determine equilibrium association constants of the complexes. Particular attention is devoted to describing how to tune an electrospray mass spectrometer using a 12-mer oligodeoxynucleotides duplex in order to perform these experiments. This protocol can then be applied to any nucleic acid structure that can be ionized with electrospray mass spectrometry.
Gabelica
Valérie
V
Physical Chemistry and Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Liège, Belgium. v.gabelica@ulg.ac.be
D053000:Analytic Sample Preparation Methods; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D008024:Ligands; D004247:DNA