title: Evidence for a role of vertebrate Disp1 in long-range Shh signaling.
abstract: Dispatched 1 (Disp1) encodes a twelve transmembrane domain protein that is required for long-range sonic hedgehog (Shh) signaling. Inhibition of Disp1 function, both by RNAi or dominant-negative constructs, prevents secretion and results in the accumulation of Shh in source cells. Measuring the Shh response in neuralized embryoid bodies (EBs) derived from embryonic stem (ES) cells, with or without Disp1 function, demonstrates an additional role for Disp1 in cells transporting Shh. Co-cultures with Shh-expressing cells revealed a significant reduction in the range of the contact-dependent Shh response in Disp1(-/-) neuralized EBs. These observations support a dual role for Disp1, not only in the secretion of Shh from the source cells, but also in the subsequent transport of Shh through tissue.
authors.affiliation: Department of Molecular and Cell Biology, University of California at Berkeley, 16 Barker Hall #3204, Berkeley, CA 94720-3204, USA.
authors.forename: L Alton
authors.initials: LA
authors.lastname: Etheridge
authors.forename: T Quinn
authors.initials: TQ
authors.lastname: Crawford
authors.forename: Shile
authors.initials: S
authors.lastname: Zhang
authors.forename: Henk
authors.initials: H
authors.lastname: Roelink
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D004285:Dogs; D053595:Embryonic Stem Cells; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D034622:RNA Interference; D051381:Rats; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014714:Vertebrates / Q000502:physiology
chemical_list: D053823:Hedgehog Proteins; D008565:Membrane Proteins