title: Cell adhesion property affected by cyclooxygenase and lipoxygenase: Opto-electric approach.
abstract: Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- and COX-2-expressing cells enhanced phosphorylation of focal adhesion kinase, but all the transfected cells showed invasion activity. However, in a real-time experiment using opto-electric approaches, transmitted cellular morphology was much different with tight adhesion being shown in COX-2 expressing cells, as imaged by differential interference contrast microscopy (DICM) and interference reflection contrast microscopy (IRCM). Furthermore, micro-impedance measurements showed a continued increase in both resistance and reactance of COX- and LOX-transfected cells, consistent with the imaging data. Our data indicate that both COX- and LOX-expressing cells have strong cell-to-cell and cell-to-substrate adhesions, and that cell imaging analysis with cell impedance data generates fully reliable results on cell adhesion measurement.
authors.affiliation: Department of Mechanical Engineering - Engineering Mechanics, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: Chang Kyoung
authors.initials: CK
authors.lastname: Choi
authors.forename: Mugdha
authors.initials: M
authors.lastname: Sukhthankar
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Anthony
authors.initials: A
authors.lastname: English
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Kihm
authors.forename: Seung Joon
authors.initials: SJ
authors.lastname: Baek
mesh_terms: D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D017097:Electric Impedance; D006801:Humans; D008853:Microscopy / Q000379:methods; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000201:enzymology / Q000473:pathology*; D014162:Transfection
chemical_list: D001093:Arachidonate 15-Lipoxygenase; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human