Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate.
We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and NDA-biogenic amine derivatives by CE in conjunction with light-emitting diode-induced fluorescence detection using poly(ethylene oxide) (PEO) solutions containing sodium dodecyl sulfate (SDS). After sample injection, via EOF 0.1% PEO prepared in 100mM TB solution (pH 9.0) containing 30 mM SDS entered a capillary filled with 0.5M TB solution (pH 10.2) containing 40 mM SDS. Under this condition, 14 NDA-amino acid and NDA-amine derivatives were separated within 16 min, with high efficiency ((1.0-3.2)x10(5) theoretical plates) and sensitivity (LODs at S/N=3 ranging from 2.06 to 19.17 nM). In the presence of SDS and PEO, analytes adsorption on the capillary wall was suppressed, leading to high efficiency and reproducibility. The intraday analysis RSD values (n=3) of the mobilities for the analytes are less than 0.52%. We have validated the practicality of this approach by quantitative determination of 9 amino acids in breast cancer cells (MCF-7) and 10 amino acids in normal epithelial cells (H184B5F5/M10). The concentrations of Tau and Gln in the MCF-7 cells were different than those in the H184B5F5/M10 cells, respectively. Our results show the potential of this approach for cancer study.
Kao
Yu-Yen
YY
Department of Chemistry, National Taiwan University, 1 Section 4, Roosevelt Road, Taipei 106, Taiwan.
Liu
Kung-Tien
KT
Huang
Ming-Feng
MF
Chiu
Tai-Chia
TC
Chang
Huan-Tsung
HT
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D001679:Biogenic Amines / Q000032:analysis* / Q000737:chemistry; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D009281:Naphthalenes / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000596:Amino Acids; D001679:Biogenic Amines; D009281:Naphthalenes; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; C027717:2,3-naphthalenedicarboxaldehyde