Chemical composition analysis of soybean oligosaccharides and its effect on ATPase activities in hyperlipidemic rats.
A water-soluble oligosaccharide was isolated from the water extract of soybean, which is widely grown worldwide due to its nutritional benefits of a full range of amino acids and a high protein content. In this study, we analysed chemical composition of soybean oligosaccharides (SBOS) using high performance liquid chromatography (HPLC). The results obtained were compared with the following sugar standards: mannose, galactose, xylose, arabinose, fucose and glucose. Results showed that SBOS was composed of galactose (65.3%), mannose (15.6%), fructose (7.8%) and glucose (8.7%). The pharmacological action of SBOS has been studied in hyperlipidemic rats fed high-fat diet. In hyperlipidemic rats, feeding with SBOS (150 and 300 mg/kg b.w.) exerted a pharmacological effect as assessed by reversal of plasma levels of lactic acid, nitric oxide (NO), vascular endothelial growth factor (VEGF) levels, and heart Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase activities. These results indicated that SBOS may have beneficial effects on the therapy of some heart diseases, such as coronary artery diseases.
Chen
Hua
H
Department of Emergency, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Li-Jun
Liu
L
Jian-Jun
Zhu
Z
Bo
Xu
X
Rui
Li
L
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D006949:Hyperlipidemias / Q000201:enzymology*; D019344:Lactic Acid / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D009569:Nitric Oxide / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D009844:Oligosaccharides; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide; D019344:Lactic Acid; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase