Effect of ultraviolet radiation on acetylcholinesterase activity in freshwater copepods.
We analyzed the effects of UV radiation (UVR) effects on acetylcholinesterase (AChE) activity in two calanoid copepods, Boeckella gibbosa and Parabroteas sarsi that inhabit Patagonian shallow lakes. We studied the effect of experimental UVR (UV-B and UV-A) exposure on AChE activity in relation to basal antioxidant capacities of both copepods. Our experiments showed that UVR can effectively depress AChE activity, although with differences between species. In both copepods AChE was affected by UV-B, whereas UV-A only affected AChE in B. gibbosa. Both copepods also differed in body elemental composition (C:N:P), photoprotecting compound content (carotenoids and mycosporine-like amino acids) and enzymatic antioxidant capacity (glutathione S-transferase [GST]). Our results suggest that when exposed to UVR, AChE activity would depend more on the antioxidant capacity (GST) and P availability for enzyme synthesis than on the photoprotective compounds.
Souza
María Sol
MS
Laboratorio de Limnología, INIBIOMA, CONICET-UNComahue, Bariloche, Argentina. ssouza@crub.uncoma.edu.ar
Balseiro
Esteban
E
Laspoumaderes
Cecilia
C
Modenutti
Beatriz
B
D000110:Acetylcholinesterase / Q000528:radiation effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D033342:Copepoda; D005618:Fresh Water; D005982:Glutathione Transferase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D005982:Glutathione Transferase; D000110:Acetylcholinesterase