title: AtMetExpress development: a phytochemical atlas of Arabidopsis development.
abstract: Plants possess many metabolic genes for the production of a wide variety of phytochemicals in a tissue-specific manner. However, the metabolic systems behind the diversity and tissue-dependent regulation still remain unknown due to incomplete characterization of phytochemicals produced in a single plant species. Thus, having a metabolome dataset in addition to the genome and transcriptome information resources would enrich our knowledge of plant secondary metabolism. Here we analyzed phytochemical accumulation during development of the model plant Arabidopsis (Arabidopsis thaliana) using liquid chromatography-mass spectrometry in samples covering many growth stages and organs. We also obtained tandem mass spectrometry spectral tags of many metabolites as a resource for elucidation of metabolite structure. These are part of the AtMetExpress metabolite accumulation atlas. Based on the dataset, we detected 1,589 metabolite signals from which the structures of 167 metabolites were elucidated. The integrated analyses with transcriptome data demonstrated that Arabidopsis produces various phytochemicals in a highly tissue-specific manner, which often accompanies the expression of key biosynthesis-related genes. We also found that a set of biosynthesis-related genes is coordinately expressed among the tissues. These data suggested that the simple mode of regulation, transcript to metabolite, is an origin of the dynamics and diversity of plant secondary metabolism.
authors.affiliation: RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.forename: Masami Y
authors.initials: MY
authors.lastname: Hirai
authors.forename: Eriko
authors.initials: E
authors.lastname: Sasaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Yonekura-Sakakibara
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Provart
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Shimada
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D057225:Data Mining; D016208:Databases, Factual; D020869:Gene Expression Profiling; D055442:Metabolome; D053719:Tandem Mass Spectrometry