Oscillations in supercoiling drive circadian gene expression in cyanobacteria.
The cyanobacterium Synechococcus elongatus PCC 7942 exhibits oscillations in mRNA transcript abundance with 24-h periodicity under continuous light conditions. The mechanism underlying these oscillations remains elusive--neither cis nor trans-factors controlling circadian gene expression phase have been identified. Here, we show that the topological status of the chromosome is highly correlated with circadian gene expression state. We also demonstrate that DNA sequence characteristics of genes that appear monotonically activated and monotonically repressed by chromosomal relaxation during the circadian cycle are similar to those of supercoiling-responsive genes in Escherichia coli. Furthermore, perturbation of superhelical status within the physiological range elicits global changes in gene expression similar to those that occur during the normal circadian cycle.
Vijayan
Vikram
V
Graduate Program in Systems Biology, Howard Hughes Medical Institute, Harvard Faculty of Arts and Sciences Center for Systems Biology, Departments of Molecular and Cellular Biology and Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Zuzow
Rick
R
O'Shea
Erin K
EK
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism; D002940:Circadian Rhythm / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D055106:Genome-Wide Association Study; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046940:Synechococcus / Q000235:genetics* / Q000378:metabolism*; D049490:Systems Biology
D017931:DNA Primers; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D012329:RNA, Bacterial; D012333:RNA, Messenger