title: New coupling strategy for radionuclide labeling of synthetic polymers.
abstract: We have developed a radiolabeling strategy for synthetic polymers based on the formation of azo dye usable for both covalent and chelating labeling modalities under mild conditions. Poly[N-(2-hydroxypropyl)methacrylamide] and poly(N-isopropyl acrylamide) were used as model polymers. N-methacryloyl tyrosinamide was introduced into the polymers and the phenolic moiety was then reacted with diazotized chelator precursors. The conjugates were radiolabeled with both the covalently bound (iodine-125) and chelated (indium-111) radionuclides in high yields and sufficient in vitro stability of the labels was proven.
authors.affiliation: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. mhruby@centrum.cz
authors.forename: Martin
authors.initials: M
authors.lastname: Hruby
authors.forename: Jan
authors.initials: J
authors.lastname: Kucka
authors.forename: Michaela
authors.initials: M
authors.lastname: Novakova
authors.forename: Hana
authors.initials: H
authors.lastname: Mackova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Vetrik
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011868:Radioisotopes / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; D011108:Polymers; D011868:Radioisotopes