Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme.
Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the phi29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme (SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 microg/ml. In addition, this enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity.
Son
Jee-Soo
JS
Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Building 200-#4204, Sillim-9 dong, Seoul, 151-742, South Korea.
Lee
Se-Jung
SJ
Jun
Soo Youn
SY
Yoon
Seong Jun
SJ
Kang
Sang Hyeon
SH
Paik
Hyoung Rok
HR
Kang
Jung Ok
JO
Choi
Yun-Jaie
YJ
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001433:Bacteriolysis; D018441:Biofilms; D002417:Cattle; D002473:Cell Wall / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016679:Genome, Viral; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000201:enzymology / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000378:metabolism; D013203:Staphylococcal Infections / Q000382:microbiology / Q000628:therapy / Q000821:virology; D013204:Staphylococcus Phages / Q000201:enzymology / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000378:metabolism / Q000821:virology*
D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D010450:Endopeptidases; C001143:endolysin