title: Nitrogen fluxes from treefrogs to tank epiphytic bromeliads: an isotopic and physiological approach.
abstract: Diverse invertebrate and vertebrate species live in association with plants of the large Neotropical family Bromeliaceae. Although previous studies have assumed that debris of associated organisms improves plant nutrition, so far little evidence supports this assumption. In this study we used isotopic ((15)N) and physiological methods to investigate if the treefrog Scinax hayii, which uses the tank epiphytic bromeliad Vriesea bituminosa as a diurnal shelter, contributes to host plant nutrition. In the field, bromeliads with frogs had higher stable N isotopic composition (delta(15)N) values than those without frogs. Similar results were obtained from a controlled greenhouse experiment. Linear mixing models showed that frog feces and dead termites used to simulate insects that eventually fall inside the bromeliad tank contributed, respectively, 27.7% (+ or - 0.07 SE) and 49.6% (+ or - 0.50 SE) of the total N of V. bituminosa. Net photosynthetic rate was higher in plants that received feces and termites than in controls; however, this effect was only detected in the rainy, but not in the dry season. These results demonstrate for the first time that vertebrates contribute to bromeliad nutrition, and that this benefit is seasonally restricted. Since amphibian-bromeliad associations occur in diverse habitats in South and Central America, this mechanism for deriving nutrients may be important in bromeliad systems throughout the Neotropics.
authors.affiliation: Departamento de Zoologia e Botânica, IBILCE, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265, São José do Rio Preto, 15054-000, SP, Brazil. gq_romero@yahoo.com.br
authors.forename: Gustavo Q
authors.initials: GQ
authors.lastname: Romero
authors.forename: Fausto
authors.initials: F
authors.lastname: Nomura
authors.forename: Ana Z
authors.initials: AZ
authors.lastname: Gonçalves
authors.forename: Natacha Y N
authors.initials: NY
authors.lastname: Dias
authors.forename: Helenice
authors.initials: H
authors.lastname: Mercier
authors.forename: Elenice de C
authors.initials: Ede C
authors.lastname: Conforto
authors.forename: Denise de C
authors.initials: Dde C
authors.lastname: Rossa-Feres
mesh_terms: D000818:Animals; D001938:Brazil; D031229:Bromeliaceae / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism; D017753:Ecosystem; D005243:Feces / Q000737:chemistry; D020049:Isoptera / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011891:Rain; D011898:Ranidae / Q000502:physiology*; D012621:Seasons
chemical_list: D009587:Nitrogen Isotopes; D010940:Plant Proteins; D002734:Chlorophyll; D009584:Nitrogen