title: Comparison of tissue microarray and full section in immunohistochemistry of gastrointestinal stromal tumors.
abstract: The aim of the present study was to compare the efficiency of tissue microarray (TMA) results using immunohistochemistry markers applied to a variety of core sizes and full sections of gastrointestinal stromal tumors (GIST) using performance measures such as core loss rate and concordance rate. Six primary antibody markers (c-Kit, CD34, smooth muscle actin (SMA), S-100, p53, and Ki-67) were used with the TMA technique to analyze 0.6 mm, 2 mm, and 3 mm punch cores of GIST samples from 67 patients. No statistical association was found between core size and loss rate (P= 0.512). TMA results for the 0.6 mm, 2 mm, and 3 mm core showed that all core sizes could statistically significantly reflect full sections with regard to c-Kit, SMA, and S-100 antibodies, but that the 3 mm core section was the most representative except for CD34. With regard to p53 and Ki-67 staining, the 0.6 mm core section was not representative, but the 2 mm and 3 mm core sections could statistically significantly represent full section results. Among them, the 3 mm core section was more accurate than the 2 mm core section. Use of a single 3 mm core size in TMA is suitable for evaluating large numbers of protein and nuclear stains with regard to immunohistochemistry for GIST.
authors.affiliation: Departments of Pathology, Kangdong Sacred-Heart Hospital, Hallym University Medical College, Seoul.
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Kwon
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Nam
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Park
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Shin
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Chan Heun
authors.initials: CH
authors.lastname: Park
authors.forename: Won Jae
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000199:Actins / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis*; D046152:Gastrointestinal Stromal Tumors / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009418:S100 Proteins / Q000096:biosynthesis; D019089:Stem Cell Factor / Q000096:biosynthesis; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D000199:Actins; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D009418:S100 Proteins; D019089:Stem Cell Factor