title: Antimicrobial peptides from the skin secretions of the South-East Asian frog Hylarana erythraea (Ranidae).
abstract: Peptidomic analysis of norepinephrine-stimulated skin secretions of the South-East Asian frog Hylarana erythraea (formerly Rana erythraea partim) has led to the identification of multiple peptides with antimicrobial activity. Structural characterization of the peptides demonstrated that they belong to the brevinin-1 (3), brevinin-2 (2), esculentin-2 (4), and temporin (1) families. The values in parentheses indicate the number of paralogs. In addition, a peptide (GVIKSVLKGVAKTVALG ML.NH(2)) was isolated that shows some structural similarity to the brevinin-2-related peptides (B2RP) previously isolated from North American frogs of the genus Lithobates. A synthetic replicate of the species B2RP showed broad-spectrum growth inhibitory activity against reference strains of Escherichia coli (MIC=12.5 microM), Staphylococcus aureus (MIC=12.5 microM) and Candida albicans (MIC=50 microM) and was active against multidrug-resistant clinical isolates of Acetinobacter baumannii (MIC in the range 6-12.5 microM). The hemolytic activity of the peptide was relatively low (LC(50)=280 microM). Phylogenetic analysis based upon the amino acid sequences of 47 brevinin-2 peptides from 17 Asian species belonging to the family Ranidae provides support for the placement of H. erythraea in the genus Hylarana.
authors.affiliation: Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al-Ain, United Arab Emirates.
authors.forename: Nadia
authors.initials: N
authors.lastname: Al-Ghaferi
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kolodziejek
authors.forename: Norbert
authors.initials: N
authors.lastname: Nowotny
authors.forename: Laurent
authors.initials: L
authors.lastname: Coquet
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Jay D
authors.initials: JD
authors.lastname: King
authors.forename: J Michael
authors.initials: JM
authors.lastname: Conlon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D011898:Ranidae; D016415:Sequence Alignment; D012867:Skin / Q000737:chemistry*
chemical_list: D023181:Antimicrobial Cationic Peptides