title: Procedures for somatic cell nuclear transfer in the rat.
abstract: Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available. The rat specific features of the oocyte have long impeded the development of SCNT. We detail here the procedures developed and optimized during the last several years for the optimization of rat cloning.
authors.affiliation: genOway, SA, Lyon, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Cozzi
authors.forename: Eryao
authors.initials: E
authors.lastname: Wang
authors.forename: Christelle
authors.initials: C
authors.lastname: Jacquet
authors.forename: Alexandre
authors.initials: A
authors.lastname: Fraichard
authors.forename: Yacine
authors.initials: Y
authors.lastname: Cherifi
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D051381:Rats / Q000235:genetics*; D017207:Rats, Sprague-Dawley