Comprehensive molecular structure of the eukaryotic ribosome.
Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2.
Taylor
Derek J
DJ
Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA.
Devkota
Batsal
B
School of Biology and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA.
Huang
Andrew D
AD
School of Biology and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA.
Topf
Maya
M
Department of Bioengineering and Therapeutical Sciences, Department of Pharmaceutical Chemistry, and California Institute of Quantitative Biosciences, Mission Bay Byers Hall, University of California at San Francisco, San Francisco, CA 94158, USA.
Narayanan
Eswar
E
Department of Bioengineering and Therapeutical Sciences, Department of Pharmaceutical Chemistry, and California Institute of Quantitative Biosciences, Mission Bay Byers Hall, University of California at San Francisco, San Francisco, CA 94158, USA.
Sali
Andrej
A
Department of Bioengineering and Therapeutical Sciences, Department of Pharmaceutical Chemistry, and California Institute of Quantitative Biosciences, Mission Bay Byers Hall, University of California at San Francisco, San Francisco, CA 94158, USA.
Harvey
Stephen C
SC
School of Biology and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA.
Frank
Joachim
J
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology and Department of Biological Sciences, Columbia University, New York, NY 10032, USA. Electronic address: jf2192@columbia.edu.
D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry*
D012335:RNA, Ribosomal