title: Complete NMR analysis of oxytocin in phosphate buffer.
abstract: Complete NMR analysis of oxytocin (OXT) in phosphate buffer was elucidated by one-dimensional (1D)- and two-dimensional (2D)-NMR techniques, which involve the assignment of peptide amide NH protons and carbamoyl NH(2) protons. The (1)H-(15)N correlation of seven amide NH protons and three carbamoyl NH(2) protons were also shown by HSQC NMR of OXT without (15)N enrichment.
authors.affiliation: Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan. ako-ohno@nihs.go.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Ohno
authors.forename: Nana
authors.initials: N
authors.lastname: Kawasaki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Fukuhara
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Okuda
authors.forename: Teruhide
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000577:Amides / Q000032:analysis / Q000737:chemistry; D002021:Buffers; D002219:Carbamates / Q000032:analysis / Q000737:chemistry; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D010120:Oxytocics / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D010121:Oxytocin / Q000032:analysis* / Q000737:chemistry* / Q000494:pharmacology; D010710:Phosphates / Q000737:chemistry*; D011522:Protons
chemical_list: D000577:Amides; D002021:Buffers; D002219:Carbamates; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010120:Oxytocics; D010710:Phosphates; D011522:Protons; D010121:Oxytocin