title: An EPR method for estimating activity of antioxidants in mouse skin using an anthralin-derived radical model.
abstract: Inhibitory effects of intravenously or orally administered antioxidants on the anthralin-derived radical generated in skin (mainly in the epidermis) of living mice by ultraviolet-A (UVA) irradiation were estimated. Anthralin was applied to the dorsal skin of living mice and the mice were then exposed to UVA. The EPR signal intensity in skin tissue strips obtained from mice after anthralin-UVA treatment was measured by an X-band EPR spectrometer. Several common antioxidants such as ascorbate, glutathione and Trolox (a vitamin E analogue) intravenously administered to mice reduced anthralin-derived radical generation. Trolox showed the most prolonged and powerful effect. Intravenous injection of a clinically used cerebral neuroprotective drug, Edarabone (Radicut), also showed depletion for the anthralin-derived radical. Oral administration of a commercialized nutritional supplement (a cocktail of 17 herbals and vitamins) also attenuated the anthralin-derived radical. The anthralin-UVA treatment model for antioxidant activity in the epidermis is a potentially feasible method to estimate activity of antioxidants in the body.
authors.affiliation: Department of Bio-functional Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Sayo
authors.initials: S
authors.lastname: Kawai
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Utsumi
mesh_terms: D000818:Animals; D000875:Anthralin / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D016877:Oxidants / Q000633:toxicity*; D010946:Plants, Medicinal; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D016877:Oxidants; D014815:Vitamins; D000875:Anthralin