title: Induction of vitellogenin and gonadal impairment in chub (Leuciscus cephalus L.) after exposure to 17beta-estradiol and testosterone.
abstract: OBJECTIVE
  A controlled laboratory study was carried out to quantify vitellogenin (VTG) concentrations in a common cyprinid freshwater fish, the chub (Leuciscus cephalus L.), exposed to steroid hormones.
  
  
  METHODS
  The effect of 17betaestradiol, testosterone and testosterone-estradiol mixture was investigated on vitellogenin induction. Gonad status was also determined.
  
  
  RESULTS
  Oral exposure to estradiol and a testosterone-estradiol mixture increased (p < 0.01) blood plasma concentrations of VTG in blood plasma of both sexes. The testosterone-estradiol mixture had a negative effect on the investigated chub gonads. The effects were signified by histological changes when compared to control fish.
  
  
  CONCLUSIONS
  Our results showed a significant VTG increase in blood plasma of both sexes, indicating that vitellogenic response in the chub is sensitive to steroid hormones.
authors.affiliation: Research Institute of Fish Culture and Hydrobiology Vodnany, University of South Bohemia, Ceske Budejovice, Czech Republic. zlabek@vurh.jcu.cz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zlabek
authors.forename: Petra
authors.initials: P
authors.lastname: Berankova
authors.forename: Tomas
authors.initials: T
authors.lastname: Randak
authors.forename: Jitka
authors.initials: J
authors.lastname: Kolarova
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
authors.forename: Hana
authors.initials: H
authors.lastname: Kroupova
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000097:blood / Q000502:physiology*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D012727:Sex Characteristics; D013737:Testis / Q000187:drug effects* / Q000502:physiology; D013739:Testosterone / Q000633:toxicity*; D014819:Vitellogenins / Q000097:blood / Q000378:metabolism*
chemical_list: D052244:Endocrine Disruptors; D014819:Vitellogenins; D013739:Testosterone; D004958:Estradiol