Fluorescence resonance energy transfer-based analysis of lipoplexes.
We describe three applications of the FRET technique to analysis of structural and thermodynamic properties of cationic lipids and their complexes with DNA. (1) Lipid mixing assay to determine the degree to which individual vesicles undergo fusion upon complex formation. (2) DNA binding assay to obtain cationic lipid-DNA binding curves for thermodynamic analysis or binding stoichiometry characterization. (3) DNA spacing assay to determine changes in DNA packing in the multilamellar lipoplex particle.
Pozharski
Edwin
E
Department of Pharmaceutical Sciences, University of Maryland, Baltimore, USA.
D001665:Binding Sites; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008561:Membrane Fusion; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
D002412:Cations; D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D004247:DNA