title: (1)H MRS detection of glycine residue of reduced glutathione in vivo.
abstract: Glutathione (GSH) is a powerful antioxidant found inside different kinds of cells, including those of the central nervous system. Detection of GSH in the human brain using (1)H MR spectroscopy is hindered by low concentration and spectral overlap with other metabolites. Previous MRS methods focused mainly on the detection of the cysteine residue (GSH-Cys) via editing schemes. This study focuses on the detection of the glycine residue (GSH-Gly), which is overlapped by glutamate and glutamine (Glx) under physiological pH and temperature. The first goal of the study was to obtain the spectral parameters for characterization of the GSH-Gly signal under physiological conditions. The second goal was to investigate a new method of separating GSH-Gly from Glx in vivo. The characterization of the signal was carried out by utilization of numerical simulations as well as experiments over a wide range of magnetic fields (4.0-14T). The proposed separation scheme utilizes J-difference editing to quantify the Glx contribution to separate it from the GSH-Gly signal. The presented method retains 100% of the GSH-Gly signal. The overall increase in signal to noise ratio of the targeted resonance is calculated to yield a significant SNR improvement compared to previously used methods that target GSH-Cys residue. This allows shorter acquisition times for in vivo human clinical studies.
authors.affiliation: Varian Incorporated, Palo Alto, CA, USA. lana.kaiser@varianinc.com
authors.forename: Lana G
authors.initials: LG
authors.lastname: Kaiser
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Marjańska
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Matson
authors.forename: Isabelle
authors.initials: I
authors.lastname: Iltis
authors.forename: Seth D
authors.initials: SD
authors.lastname: Bush
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Soher
authors.forename: Susanne
authors.initials: S
authors.lastname: Mueller
authors.forename: Karl
authors.initials: K
authors.lastname: Young
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry; D003627:Data Interpretation, Statistical; D004574:Electromagnetic Fields; D005978:Glutathione / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013696:Temperature
chemical_list: D005978:Glutathione; D003545:Cysteine; D005998:Glycine