title: Frequency of bacille Calmette-Guerin (BCG) and Mycobacterium tuberculosis in tissue biopsy specimens of children vaccinated with BCG.
abstract: Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the mycobacterial species. The overall sensitivity of the assay was 71.5%. The prevalence rates of BCG, Mycobacterium tuberculosis, and other mycobacteria in the positive results were 80% (12/15), 13% (2/15), and 7% (1/15), respectively.
authors.affiliation: Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Monajemzadeh
authors.forename: Reza
authors.initials: R
authors.lastname: Shahsiah
authors.forename: Abdolmajid
authors.initials: A
authors.lastname: Zarei
authors.forename: Alireza Alai
authors.initials: AA
authors.lastname: Alamooti
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mahjoub
authors.forename: Setareh
authors.initials: S
authors.lastname: Mamishi
authors.forename: Ghamartaj
authors.initials: G
authors.lastname: Khotai
authors.forename: Reza
authors.initials: R
authors.lastname: Pazira
authors.forename: Neda
authors.initials: N
authors.lastname: Eram
mesh_terms: D001500:BCG Vaccine / Q000008:administration & dosage*; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006785:Hospitals, University; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007492:Iran / Q000453:epidemiology; D008297:Male; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology*
chemical_list: D001500:BCG Vaccine; D004269:DNA, Bacterial