title: An anti-DLK1 monoclonal antibody produced using ELISA and hybridoma techniques.
abstract: DLK1 is a newly identified prognostic factor associated with liver cancer survival. To prepare specific monoclonal antibody (MAb) against DLK1, cDNA of DLK1 was cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against DLK1 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 6D6 had specific binding ability with DLK1 protein in eukaryotic cells and cancer tissues. This MAb will be a helpful tool for the detection of DLK1 protein in the tissues and serum of liver cancer and other cancer patients.
authors.affiliation: Nuclear Medicine Department, Peking University No. 3 Hospital, Peking, China. jinzhonghui@bjmu.edu.cn
authors.forename: Zhong-hui
authors.initials: ZH
authors.lastname: Jin
authors.forename: Li-zhe
authors.initials: LZ
authors.lastname: Zhao
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weifang
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins