Antiproliferative activity of Larrea divaricata Cav. on lymphoma cell line: participation of hydrogen peroxide in its action.
Larrea divaricata is a plant used in Argentina. The aim was to analyze the participation of H2O2 in the antiproliferative effect of L divaricata on BW5147 cells. Different studies were performed: proliferation, viability, nitrite production, apoptosis, oxygen reducing activity, NF-kappa B translocation, H2O2 production, H2O2 scavenging activity, and effect on exogenous superoxide dismutase. The extract decreased proliferation and induced apoptosis through P-38 and ERK pathways. The extract activated Mn2+-SOD, increasing H2O2, which was implicated in the antiproliferative mechanism by the increase of Nitric oxide (NO). New drugs, which can increase H2O2, could be a therapeutic strategy for the treatment of cancer.
Davicino
Roberto
R
IQUIMEFA (UBA-CONICET), School of Pharmacy and Biochemistry, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
Manuele
Maria Gabriela
MG
Turner
Sebastian
S
Ferraro
Graciela
G
Anesini
Claudia
C
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism*; D032442:Larrea; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D009573:Nitrites; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D048051:p38 Mitogen-Activated Protein Kinases; D020930:MAP Kinase Kinase Kinases