Induction of papillary carcinoma in human ovarian surface epithelial cells using combined genetic elements and peritoneal microenvironment.
Papillary differentiation is one of the most common histological features of ovarian cancer, although the underlying mechanism that leads to such differentiation is not known. We hypothesized that human ovarian surface epithelial cells can be transformed into carcinoma with papillary differentiation by overexpressing HER2/neu in these cells. Mice were injected either subcutaneously or intraperitoneally with two immortalized human ovarian surface epithelial cell lines after enforced expression of HER-2/neu. Mice subcutaneously injected with tumor cells from either the T29Nt or T80Nt developed undifferentiated carcinomas. In contrast, mice injected intraperitoneally with T29Nt cells developed papillary carcinoma, and those injected intraperitoneally with T80Nt cells developed undifferentiated carcinoma. Our results demonstrate that ovarian surface epithelial cells can develop into papillary carcinoma in mice, and that the induction of papillary differentiation depends not only on specific genetic modifications but also on the tumor microenvironment and epithelial cell type from ovary from different patients.
Zheng
Jingfang
J
Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Mercado-Uribe
Imelda
I
Rosen
Daniel G
DG
Chang
Bin
B
Liu
Peishu
P
Yang
Gong
G
Malpica
Anais
A
Naora
Honami
H
Auersperg
Nelly
N
Mills
Gordon B
GB
Bast
Robert C
RC
Liu
Jinsong
J
D000818:Animals; D015153:Blotting, Western; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D010053:Ovary / Q000166:cytology*; D010537:Peritoneum / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000502:physiology
D018719:Erb-b2 Receptor Tyrosine Kinases