In vitro detection and primary cultivation of bacteria producing materials inhibitory to ruminal methanogens.
A novel method for screening bacterial isolates for their potential to inhibit the growth of ruminal methanogenic Archaea was developed using a modification of the soft agar overlay technique, formally used for the isolation of lytic bacteriophages. This method may be used in the specific, hydrogen-rich conditions required for the growth of ruminal methanogenic Archaea.
Gilbert
Rosalind A
RA
Animal Research Institute, Queensland Primary Industries and Fisheries, Yeerongpilly, Queensland, 4105, Australia. ros.gilbert@deedi.qld.gov.au
Ouwerkerk
Diane
D
Zhang
Li Hua
LH
Klieve
Athol V
AV
D000818:Animals; D000898:Antibiosis; D001105:Archaea / Q000254:growth & development* / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D008697:Methane / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
D008697:Methane