title: The effect of hysteroscopic polypectomy on the concentrations of endometrial implantation factors in uterine flushings.
abstract: Endometrial polyps have been associated with infertility and early pregnancy loss. The aim of this study was to investigate the effect of hysteroscopic polypectomy on the concentrations of endometrial implantation factors in uterine flushings. Pre-menopausal women with a certain diagnosis of endometrial polyp on contrast-enhanced transvaginal ultrasound scan were recruited into this prospective study. In all women, paired samples of uterine flushings were obtained on the same day of the menstrual cycle prior to and post hysteroscopic polypectomy. Enzyme-linked immunoassays were performed to analyse glycodelin, interleukin-6 (IL-6), interleukin-10 (IL-10), tumour necrosis factor alpha (TNFalpha) and osteopontin, whilst immunoradiometric assay was used to analyse insulin-like growth factor binding protein-1 (IGFBP-1). Concentrations of IGFBP-1, TNFalpha and osteopontin in uterine flushings were significantly lower in the mid-secretory phase prior to polypectomy in comparison to the measurements obtained after complete surgical removal of the polyp (P < 0.05). There were no differences in the concentrations of glycodelin, IL-6 and IL-10 in paired samples prior to and post-polypectomy. The presence of endometrial polyps is associated with decreased mid-secretory concentrations of IGFBP-1, TNFalpha and osteopontin, which are reversed following surgical polypectomy.
authors.affiliation: Early Pregnancy and Gynaecology Assessment Unit, King's College Hospital, London, UK. jbennagi@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Ben-Nagi
authors.forename: J
authors.initials: J
authors.lastname: Miell
authors.forename: J
authors.initials: J
authors.lastname: Yazbek
authors.forename: T
authors.initials: T
authors.lastname: Holland
authors.forename: D
authors.initials: D
authors.lastname: Jurkovic
mesh_terms: D000328:Adult; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008875:Middle Aged; D053495:Osteopontin / Q000378:metabolism; D011127:Polyps / Q000601:surgery*; D011257:Pregnancy Proteins / Q000378:metabolism; D011446:Prospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014591:Uterine Diseases / Q000601:surgery*; D014599:Uterus / Q000378:metabolism*
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C032231:IGFBP1 protein, human; C508609:IL10 protein, human; C508600:IL6 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D015850:Interleukin-6; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D014409:Tumor Necrosis Factor-alpha; D053495:Osteopontin; D016753:Interleukin-10