Preconditioning and postconditioning reduce hepatic ischemia-reperfusion injury in rats.
BACKGROUND
Ischemia-reperfusion injury occurs when ischemic tissues or organs suffer from further functional and structural damage when their blood supply recovers. This study aimed to contrast the protective effects of ischemic preconditioning and ischemic postconditioning in hepatic ischemia-reperfusion injury in rats.


METHODS
Thirty-two healthy male Wistar rats were randomly divided into four groups: sham-operated (SO), ischemia-reperfusion (IR), ischemic preconditioning (I-pre), and ischemic postconditioning (I-post). Blood samples and hepatic tissue were taken from all groups after the experiments.


RESULTS
There were significant differences between the IR, I-pre and I-post groups in alanine aminotransferase and aspartate aminotransferase levels, NF-kappaB p65 expression, apoptosis index and superoxide dismutase activity in hepatic tissue. There were no significant differences between the I-pre and I-post groups.


CONCLUSIONS
Ischemic postconditioning and ischemic preconditioning reduce hepatic ischemia-reperfusion injury, but in clinical practice the former is a more appropriate choice.
Zhang
Wan-Xing
WX
Department of Hepatobiliary Surgery, Hebei Provincial General Hospital, Shijiazhuang 050051, China. zhangwx12@hotmail.com
Yin
Wen
W
Zhang
Lei
L
Wang
Lan-Hui
LH
Bao
Lei
L
Tuo
Hong-Fang
HF
Zhou
Li-Fang
LF
Wang
Chun-Cheng
CC
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
D015415:Biomarkers; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase