title: 13C labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae.
abstract: The structure of zeamine, a novel polyamino-amide antibiotic metabolite of Dickeya zeae has been established by NMR and detailed MS analyses; labelling studies with (13)C-labelled acetates suggest that the repeating secondary amine containing motif may be biosynthesised via a modular PKS containing aminotransferase domains.
authors.affiliation: Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673. jwu@imcb.a-star.edu.sg
authors.forename: Jien
authors.initials: J
authors.lastname: Wu
authors.forename: Hai-Bao
authors.initials: HB
authors.lastname: Zhang
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Xu
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Cox
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Simpson
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Zhang
mesh_terms: D000586:Amination; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry; D004755:Enterobacteriaceae / Q000201:enzymology*; D018942:Macrolides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D018942:Macrolides; D011073:Polyamines; C547236:zeamine; D048630:Polyketide Synthases