title: Tissue disaggregation.
abstract: A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent.
authors.affiliation: Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005557:Formaldehyde; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D016707:Tissue Fixation
chemical_list: D005557:Formaldehyde; D010447:Peptide Hydrolases