title: Phosphoric acid/sodium hypochlorite mixture as dentin conditioner: a new approach.
abstract: OBJECTIVE
  To investigate the effect of a phosphoric acid/sodium hypochlorite (NaOCl) mixture as a dentin conditioner on shear bond strength, the resin/dentin interface, as well as the surface area and tubular diameter of dentin.
  
  
  METHODS
  Thirty human molars were prepared for shear bond strength testing. The teeth were randomly divided into five groups according to the method of dentin conditioning. In group I, dentin was conditioned with 37% phosphoric acid, in group II dentin was conditioned with 37% phosphoric acid followed by application of 5.25% NaOCl, and in groups III, IV, and V, dentin was conditioned with a phosphoric acid/NaOCl mixture for 15, 30, and 60 s, respectively. A resin-based restorative composite disk was bonded to the occlusal surface of each tooth and shear bond strength was measured in a universal testing machine. The resin/dentin interface was evaluated using a scanning electron microscope (SEM). The surface area and tubular diameter for different conditioning methods were assessed using atomic force microscopy (AFM). The data were submitted to one-way ANOVA at a significance level of 0.05 followed by the Tukey-Kramer Multiple comparisons test.
  
  
  RESULTS
  Group III showed the highest shear bond strength value (36 MPa), which was significantly higher than groups I and II. SEM micrographs revealed hybrid layer formation for all groups except group II. AFM results showed that groups II and V have significantly higher surface areas and dentinal tubular diameters than other groups.
  
  
  CONCLUSIONS
  The use of a phosphoric acid/NaOCl mixture as dentin conditioner improved the bond strength of an etch-and-rinse single-bottle adhesive to dentin.
authors.affiliation: Dental Biomaterials Department, Faculty of Dentistry, Ain-Shams University, Cairo, Egypt.
authors.forename: Mohammed S
authors.initials: MS
authors.lastname: Nassif
authors.forename: Dalia I
authors.initials: DI
authors.lastname: El-Korashy
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D003094:Collagen / Q000187:drug effects; D003188:Composite Resins; D001840:Dental Bonding / Q000379:methods*; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects*; D003806:Dentin Permeability; D004338:Drug Combinations; D006801:Humans; D008422:Materials Testing; D008964:Molar, Third; D010756:Phosphoric Acids / Q000494:pharmacology*; D033081:Shear Strength; D012973:Sodium Hypochlorite / Q000494:pharmacology*
chemical_list: D003188:Composite Resins; D004338:Drug Combinations; D010756:Phosphoric Acids; D003094:Collagen; D012973:Sodium Hypochlorite; C030242:phosphoric acid