title: Retrograde arterial flush of the liver graft in living donor liver transplantation.
abstract: Formal hepatic arterial flush to preserve the liver graft in living donor liver transplantation (LDLT) is not recommended by most transplant centers because direct cannulation may injure the intima of the hepatic artery. The authors describe a method of retrograde arterial flush of the liver graft without arterial cannulation by hepatic venous outflow occlusion (HVOO) in LDLT. First, we proved no backflow of the hepatic artery without HVOO by portal flush to pig livers. Then we used HVOO on 15 LDLT cases (Group HVOO). The results were compared with those of 24 counterpart LDLT cases (Group non-HVOO) without hepatic artery flush. The two-week posttransplantational liver functions were not different between two groups except that the day-three and day-seven serum bilirubin levels were lower in Group HVOO (day-three total bilirubin: 4.99 +/- 4.04 mg/dl versus 7.65 +/- 4.33 mg/dl, p =.016; day-seven total bilirubin: 5.06 +/- 5.02 mg/dl versus 9.57 +/- 6.09, p =.005). The rates of vascular complications, six-month graft survival, and biliary anastomotic stricture in Group HVOO were 0, 93.3, and 13.3% respectively, which were not different from those of Group non-HVOO. In summary, to avoid intima injury, the retrograde arterial flush of liver graft by HVOO is safe in LDLT. The short-term results showed the effect of decreasing early functional cholestasis but the long-term benefits need further evaluation, especially with regards to biliary anastomotic complications.
authors.affiliation: Department of Surgery, Taipei Veterans General Hospital, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan. csliu@vghtpe.gov.tw
authors.forename: Chinsu
authors.initials: C
authors.lastname: Liu
authors.forename: Che-Chuan
authors.initials: CC
authors.lastname: Loong
authors.forename: Cheng-Yuan
authors.initials: CY
authors.lastname: Hsia
authors.forename: Mei-Yung
authors.initials: MY
authors.lastname: Tsou
authors.forename: Hsin-Lin
authors.initials: HL
authors.lastname: Tsai
authors.forename: Choufu
authors.initials: C
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002404:Catheterization / Q000009:adverse effects; D002648:Child; D002675:Child, Preschool; D005260:Female; D006085:Graft Survival / Q000502:physiology; D006499:Hepatic Artery / Q000293:injuries / Q000502:physiology*; D006504:Hepatic Veno-Occlusive Disease / Q000517:prevention & control; D006801:Humans; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000379:methods*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D023421:Models, Animal; D012039:Regional Blood Flow / Q000502:physiology; D013552:Swine; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D001663:Bilirubin