Capillary electrophoresis of chitooligosaccharides in acidic solution: simple determination using a quaternary-ammonium-modified column and indirect photometric detection with crystal violet.
Five chitosan oligosaccharides were separated in acidic aqueous solution by capillary electrophoresis (CE) with indirect photometric detection using a positively coated capillary. Electrophoretic mobility of the chitooligosaccharides (COSs) depended on the number of monomer units in acidic aqueous solution, similar to other polyelectrolyte oligomers. The separation was developed in nitric acid aqueous solution at pH 3.0 with 1 mM Crystal Violet, using a capillary positively coated with N-trimethoxypropyl-N,N,N-trimethylammonium chloride. The limit of the detection for chitooligosaccharides with two to six saccharide chains was less than 5 microM. CE determination of an enzymatically hydrolyzed COS agreed with results from HPLC.
Hattori
Toshiaki
T
Department of Materials Science, Toyohashi University of Technology, Hibarigaoka 1-1, Tempaku, Toyohashi 441-8580, Japan. thattori@tutms.tut.ac.jp
Anraku
Nobuhiro
N
Kato
Ryo
R
D048271:Chitosan / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005840:Gentian Violet / Q000737:chemistry*; D006868:Hydrolysis; D009038:Motion; D017942:Nitric Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D009861:Onium Compounds / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010777:Photochemistry / Q000295:instrumentation*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012015:Reference Standards; D012996:Solutions
D009844:Oligosaccharides; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D000644:Quaternary Ammonium Compounds; D012996:Solutions; D017942:Nitric Acid; D048271:Chitosan; D005840:Gentian Violet; C013289:tetraphenylphosphonium