title: Age-related effects on hippocampal precursor cell subpopulations and neurogenesis.
abstract: Hippocampal neurogenesis continuously declines in the aging brain but only little is known about age-related alterations in the subgranular zone (SGZ) of the dentate gyrus which accommodates different subpopulations of precursor cells. Here, we examined the age-related effects on total number and proliferation rate of distinct precursor cell populations in the dentate gyrus of 3 and 16 months old transgenic pNestin-GFP mice. Following a single injection of bromodeoxyuridine (BrdU) we observed a significant reduction of all proliferating precursor subtypes in aged mice compared to young controls. Stereological analysis further revealed that this decreased proliferation was not only caused by a general reduction in total number of precursor subtypes but also by a subtype-specific alteration of the proliferation rate. Whereas radial glia-like and early neuronal precursor cells demonstrate decreased proliferation rates, no difference was found for doublecortin-positive precursors. Additional long-term experiments further revealed that these age-related alterations in the proliferative zone were accompanied by a strongly decreased neurogenesis while hippocampal function was not impaired.
authors.affiliation: Department of Neurology, University Hospital Jena, Erlanger Allee 101, D-07747 Jena, Germany.
authors.forename: Josephine
authors.initials: J
authors.lastname: Walter
authors.forename: Silke
authors.initials: S
authors.lastname: Keiner
authors.forename: Otto W
authors.initials: OW
authors.lastname: Witte
authors.forename: Christoph
authors.initials: C
authors.lastname: Redecker
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D000089622:Doublecortin Domain Proteins; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology*; D020287:In Situ Nick-End Labeling; D007211:Indoles; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D059032:Learning Curve; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D058953:Neural Stem Cells / Q000145:classification* / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D000089622:Doublecortin Domain Proteins; D007211:Indoles; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D009479:Neuropeptides; D049452:Green Fluorescent Proteins; C007293:DAPI; D001973:Bromodeoxyuridine