title: Pheno- and genotypic features of Epstein-Barr virus associated B-cell lymphoproliferations in peripheral T-cell lymphomas.
abstract: Among the 300 peripheral T-cell lymphomas (PTCL) searched for EBV positive non-resting B-cells by EBER in situ hybridization 12 have been identified with various forms of EBV-driven B-cell proliferation. This could be categorized into three major forms. i. In the first form scattered immature, mononuclear B-cells of immuno-, centroblastic type with CD20+. CD30+ CD45+, LMP1+ phenotype, reactive appearance and polyclonal immunoglobulin heavy chains gene rearrangement (IgH-R) were admixed to the PTCL cells. ii. The second form mimicked diffuse large B-cell lymphoma as homogenous sheets, largely demarcated from the PTCL, of mononuclear, immature B-cell of CD20+, CD30+, CD45+, LMP1+, EBNA-2+ phenotype but with lack of monoclonal IgH-R were present. iii. In the third form scattered Hodgkin-Reed-Sternberg (HRS) type of cells were noticed which exhibited the CD15+/-, CD20-/+, CD30+, CD45-, LMP1+, EBNA-2- phenotype and in 50% showed clonal IgH gene rearrangement in whole tissue DNA extract. The IgH associated transcription factors' (OCT2, BOB.1/OBF.1, PU.1) expression patterns in these cells corresponded to those of HRS cells in cHL. Based on analysis of 65 PTCLs, we have identified in the positive cases a highly significant increase of EBV+ small, reactive, resting B-cell compartment (75.9 / 100 HPF in PTCL vs. 1.5 / 100 HPF in control lymph nodes) likely to be due to the decreased immune surveillance. This progressive accumulation of EBV+ by-stander B-cell population in PTCLs might be the source of various B-cell proliferations, which in any form represent major diagnostic pitfalls and require a careful differential diagnostic procedure.
authors.affiliation: Department of Pathology, University of Pécs Medical Center, 12 Szigeti St., Pécs, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Smuk
authors.forename: Arpád
authors.initials: A
authors.lastname: Illés
authors.forename: Katalin
authors.initials: K
authors.lastname: Keresztes
authors.forename: László
authors.initials: L
authors.lastname: Kereskai
authors.forename: Balázs
authors.initials: B
authors.lastname: Márton
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Nagy
authors.forename: Agnes
authors.initials: A
authors.lastname: Lacza
authors.forename: László
authors.initials: L
authors.lastname: Pajor
mesh_terms: D001402:B-Lymphocytes / Q000473:pathology* / Q000821:virology*; D003937:Diagnosis, Differential; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000276:immunology / Q000473:pathology; D015322:Gene Rearrangement, B-Lymphocyte / Q000276:immunology; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology; D005838:Genotype; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology
chemical_list: C099537:Epstein-Barr virus encoded RNA 1; D007143:Immunoglobulin Heavy Chains; D012367:RNA, Viral; D016692:Receptors, Antigen, T-Cell, gamma-delta