Biomolecule detection with peroxidase-mimicking DNAzymes; expanding detection modality with fluorogenic compounds.
The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing substrate for the fluorometric detection of bioanalytes using peroxidase-mimicking G-quadruplex DNAzymes.
Nakayama
Shizuka
S
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Sintim
Herman O
HO
D021881:DNA, Catalytic / Q000378:metabolism*; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D008962:Models, Theoretical; D015394:Molecular Structure; D010544:Peroxidases / Q000378:metabolism*
C110400:2',7'-dichlorodihydrofluorescein diacetate; D021881:DNA, Catalytic; D005452:Fluoresceins; D010544:Peroxidases