Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa.
Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F(1), and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC(3)F(1) plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice.
Guo
SiBin
S
National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, China.
Qin
FaLan
F
Zhang
DuanPin
D
Lin
XingHua
X
D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D014644:Genetic Variation; D006823:Hybrid Vigor / Q000235:genetics; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D040641:Quantitative Trait Loci / Q000235:genetics; D013045:Species Specificity; D014974:Xanthomonas / Q000254:growth & development
D018744:DNA, Plant