Changes in gene and protein expression in magnetic field-treated human glioma cells.
Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 muT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 muT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.
Savage
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National Institute for Occupational Safety and Health (NIOSH), Cincinnati, Ohio, 45226.
Kanitz
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Lotz
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Conover
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Hennessey
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Hanneman
W H
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Witzmann
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