Procedures for somatic cell nuclear transfer in the rat.
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available. The rat specific features of the oocyte have long impeded the development of SCNT. We detail here the procedures developed and optimized during the last several years for the optimization of rat cloning.
Cozzi
Jean
J
genOway, SA, Lyon, France.
Wang
Eryao
E
Jacquet
Christelle
C
Fraichard
Alexandre
A
Cherifi
Yacine
Y
Zhou
Qi
Q
D000818:Animals; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D051381:Rats / Q000235:genetics*; D017207:Rats, Sprague-Dawley