title: Syntheses, characterization and antitumor activities of transition metal complexes with isoflavone.
abstract: Five new complexes were synthesized by the reaction of 4'-methoxy-5,7-dihydroxy-isoflavone ligand (a) with transition metal ions zinc (Zn(2+)) (complex b), manganese (Mn(2+)) (complex c), copper (Cu(2+)) (complex d), cobalt (Co(2+)) (complex e) and nickel (Ni(2+)) (complex f). The composition of the complexes has been characterized by elemental analysis, IR, mass spectrometry (MS) and (1)H NMR spectrometric techniques. Their antitumor properties were evaluated against five human cancer cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The results indicated that the complexes possess higher growth inhibitory effects than free isoflavone and corresponding metal ions. Complex c and f showed greater antitumor activity and selectivity than other described complexes, even more effective than the positive control cisplatin against the selected cell lines. In addition, DNA flow cytometric analysis demonstrated that complexes display a significant G(2)/M phase arrest, which then progressed to early apoptosis as detected by flow cytometry after double-staining with annexin V and propidium iodide (PI).
authors.affiliation: School of Pharmacy, Wenzhou Medical College, Wenzhou 325 035, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Tang
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Sun
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Qiu
authors.forename: Guang
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007529:Isoflavones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure; D028561:Transition Elements / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D007529:Isoflavones; D028561:Transition Elements