title: The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis.
abstract: Cilia play important roles in many developmental and physiological processes. However, the genetic and cell biological control of ciliogenesis remains poorly understood. Here, we show that the zebrafish iguana gene is required for differentiation of primary cilia. iguana encodes a zinc finger and coiled-coil containing protein, previously implicated in Hedgehog signaling. We now argue that aberrant Hedgehog activity in iguana -deficient zebrafish arises from their profound lack of primary cilia. By contrast, the requirement of iguana for motile cilia formation is less obligatory. In the absence of iguana function, basal bodies can migrate to the cell surface and appear to engage with the apical membrane. However, formation of ciliary pits and axonemal outgrowth is completely inhibited. Iguana localizes to the base of primary and motile cilia, in the immediate vicinity or closely associated with the basal bodies. These findings identify the Iguana protein as a novel and critical component of ciliogenesis.
authors.affiliation: Institute of Molecular and Cell Biology, Cancer and Developmental Cell Biology Division, Proteos, Singapore.
authors.forename: Shang Yew
authors.initials: SY
authors.lastname: Tay
authors.forename: Xianwen
authors.initials: X
authors.lastname: Yu
authors.forename: Kangli Noel
authors.initials: KN
authors.lastname: Wong
authors.forename: Pallavi
authors.initials: P
authors.lastname: Panse
authors.forename: Chee Peng
authors.initials: CP
authors.lastname: Ng
authors.forename: Sudipto
authors.initials: S
authors.lastname: Roy
mesh_terms: D000818:Animals; D054468:Axoneme / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D002923:Cilia / Q000502:physiology*; D015513:Oncogene Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D015027:Zebrafish; D000071676:Zinc Finger Protein GLI1
chemical_list: D002352:Carrier Proteins; C488008:Dzip1 protein, zebrafish; D015513:Oncogene Proteins; D015534:Trans-Activators; D000071676:Zinc Finger Protein GLI1