title: Cleavable linker for photo-cross-linked small-molecule affinity matrix.
abstract: The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.
authors.affiliation: Tohoku University, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kanoh@mail.pharm.tohoku.ac
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanoh
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takayama
authors.forename: Kaori
authors.initials: K
authors.lastname: Honda
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriya
authors.forename: Takayuki
authors.initials: T
authors.lastname: Teruya
authors.forename: Siro
authors.initials: S
authors.lastname: Simizu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Iwabuchi
mesh_terms: D001665:Binding Sites; D015153:Blotting, Western; D002853:Chromatography, Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D013058:Mass Spectrometry; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry*; D010777:Photochemistry; D011485:Protein Binding; D054648:Protein Phosphatase 2 / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D004220:Disulfides; D004800:Enzymes, Immobilized; D008024:Ligands; D009943:Organophosphorus Compounds; D019920:Photoaffinity Labels; C030011:carbene; D012685:Sepharose; D054648:Protein Phosphatase 2; D008697:Methane