title: Inhibition of enhanced green fluorescent protein expression by (dextran-hexamethylenediisocyanate)-g-polyethylenimine/siRNA complexes.
abstract: Small interfering RNA (siRNA), as a potential tool for gene therapy, requires suitable vehicles for stable complexation, protection, low cytotoxicity and high efficiency of gene knockdown. Here, a new polycation, (dextran-hexamethylenediisocyanate)-g-polyethylenimine ((Dex-HMDI)-g-PEI), was used as a siRNA vector to knock down the expression of Enhanced Green Fluorescent Protein (EGFP). It was found that the complexation of siRNA with the polycation can significantly knock down the expression of EGFP, but has no remarkable effect on the expression of luciferase.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xiao
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Sun
authors.forename: Han
authors.initials: H
authors.lastname: Cheng
authors.forename: Xuan
authors.initials: X
authors.lastname: Zeng
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
mesh_terms: D002460:Cell Line; D003485:Cyanates / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D017953:Isocyanates; D008156:Luciferases / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection
chemical_list: D003485:Cyanates; D003911:Dextrans; D017953:Isocyanates; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; C015262:1,6-hexamethylene diisocyanate; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D008156:Luciferases