Anti-inflammatory evaluation of Coronopus didymus in the pleurisy and paw oedema models in mice.
BACKGROUND
Mastrunço (Coronopus didymus--CD) is currently considered as a medicinal specie often used in Brazil, especially in southeast region, for the treatment of several diseases in which pain and inflammation are common. Treatment with the plant can be done by infusion, decoction, or through food. The aim of this study was: to investigate the anti-inflammatory effect of hydroalcoholic extract obtained from the leaves of CD following the traditional procedure.


METHODS
The anti-inflammatory activity was determined using mouse of pleurisy and paw oedema models, both process being induced by different flogistic agents such as: carrageenan (Cg), bradykinin (BK), histamine (HIS), substance P (SP), dextran (DEX) or prostaglandin E(2) (PGE(2)). We evaluated the effect of CD (200-600 mg/kg) administered by oral route (p.o.) upon leukocytes migration, myeloperoxidase (MPO), and adenosine-deaminase (ADA) activities and nitric oxide (NO) levels.


RESULTS
CD (200-600 mg/kg) inhibited the leukocytes by 60.0+/-1.42%, neutrophils by 82.75+/-1.29%, MPO by 42.30+/-4.23%, and ADA activities by 57.89+/-1.94%, as well as NO levels by 64.28+/-2.15% in Cg induced pleurisy. CD also inhibited total and differential leukocytes in the pleurisy induced by BK (1.30+/-0.11/0.29+/-0.02), HIS (1.20+/-0.09/0.42+/-0.05) and SP (0.74+/-0.06/0.14+/-0.01). In addition, CD was effective in reducing paw oedema induced by Cg by 72.79+/-1.13%, SP by 68.26.+/-0.78%, BK by 66.66.+/-0.77%, PGE(2) by 53.346.+/-1.18 and DEX by 65.14+/-2.35%.


CONCLUSIONS
Several mechanisms, including the inhibition of enzymes (MPO and ADA) and mediators (BK, HIS, SP, NO and PGE(2)) release and/or action, appear to account for the anti-inflammatory effect of Coronopus didymus.
Busnardo
Tereza Cristina Pamplona Mosimann
TC
Centro de Ciências da Saúde, CCS, Mestrado em Ciências Farmacêuticas Universidade do Vale do Itajaí, UNIVALI. Itajaí, SC, Brazil.
Padoani
Cristina
C
Mora
Ticiana Camila
TC
Biavatti
Maique Weber
MW
Fröde
Tânia Silvia
TS
Bürger
Cristiani
C
Claudino
Vanessa D
VD
Dalmarco
Eduardo Monguilhote
EM
de Souza
Márcia Maria
MM
D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001920:Bradykinin / Q000494:pharmacology; D001938:Brazil; D002351:Carrageenan / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D006632:Histamine / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D009504:Neutrophils / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology; D009195:Peroxidase / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D013373:Substance P / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D009569:Nitric Oxide; D013373:Substance P; D006632:Histamine; D002351:Carrageenan; D009195:Peroxidase; D000243:Adenosine Deaminase; D001920:Bradykinin