Secreted semaphorins control spine distribution and morphogenesis in the postnatal CNS.
The majority of excitatory synapses in the mammalian CNS (central nervous system) are formed on dendritic spines, and spine morphology and distribution are critical for synaptic transmission, synaptic integration and plasticity. Here, we show that a secreted semaphorin, Sema3F, is a negative regulator of spine development and synaptic structure. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components neuropilin-2 (Npn-2, also known as Nrp2) and plexin A3 (PlexA3, also known as Plxna3), exhibit increased dentate gyrus (DG) granule cell (GC) and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in Npn-2(-/-) brain slices cortical layer V and DG GCs exhibit increased mEPSC (miniature excitatory postsynaptic current) frequency. In contrast, a distinct Sema3A-Npn-1/PlexA4 signalling cascade controls basal dendritic arborization in layer V cortical neurons, but does not influence spine morphogenesis or distribution. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Npn-2 to apical dendrites and of Npn-1 (also known as Nrp1) to all dendrites of cortical pyramidal neurons. Therefore, Sema3F signalling controls spine distribution along select dendritic processes, and distinct secreted semaphorin signalling events orchestrate CNS connectivity through the differential control of spine morphogenesis, synapse formation, and the elaboration of dendritic morphology.
Tran
Tracy S
TS
Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Rubio
Maria E
ME
Clem
Roger L
RL
Johnson
Dontais
D
Case
Lauren
L
Tessier-Lavigne
Marc
M
Huganir
Richard L
RL
Ginty
David D
DD
Kolodkin
Alex L
AL
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D039942:Neuropilin-1 / Q000378:metabolism; D039943:Neuropilin-2 / Q000378:metabolism; D017966:Pyramidal Cells / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000494:pharmacology; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D013569:Synapses / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D039943:Neuropilin-2; D011994:Recombinant Proteins; D039961:Semaphorins; D039942:Neuropilin-1