[Gene profiling after knocking-down the expression of NS gene in prostate cancer PC-3 cells].
OBJECTIVE
To screen the genes and possible signal transduction pathways involved in the mechanism of nucleostemin (NS) in the proliferation of prostate cancer.


METHODS
Oligonucleotide DNA microarray was used to screen the genome changes after knocking-down expression of NS in PC-3 cells and quantitative real-time PCR was used to further confirm the important differentially expressed genes.


RESULTS
219 differentially expressed genes were found and theses genes were involved in cell cycle, cell proliferation, signal transduction, cell apoptosis and cell differentiation, etc. INK4 family genes (p15, p16, p18) were up-regulated and cyclin D1, HDAC1 were down-regulated, the main action points were CDK4/6-cyclin D and pRb-E2F1 complexes.


CONCLUSIONS
NS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. NS is an important G(1)/S checkpoint regulator and its regulatory activity has been certified at gene level.
Liu
Ran-lu
RL
Department of Urology, Second Hospital, Tianjin Medical University & Tianjin Institute of Urology, Tianjin 300211, China.
Xu
Yong
Y
Zhang
Zhi-hong
ZH
Wang
Meng
M
Sun
Jian-tao
JT
Zhang
Yue
Y
Li
Sheng-zhi
SZ
D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D056284:Histone Deacetylase 1 / Q000378:metabolism; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D015398:Signal Transduction
D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D019938:Cyclin D1; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D019204:GTP-Binding Proteins