Characteristics of biofilms from urinary tract catheters and presence of biofilm-related components in Escherichia coli.
Long term catheterization of the urinary tract leads to bacterial colonization of the urine, whereby adherence to the catheter surface is a major determinative factor for colonization. Collection of bacterial isolates from urine and urinary catheters of 45 patients showed multi-species catheter-colonization, while Escherichia coli isolates were frequently found in the urine in high numbers. Biofilm formation of catheter and urine-derived E. coli isolates was associated with the presence of the fluA gene, loss of O-antigen, and expression of type 1 fimbriae. The second messenger cyclic di-GMP (cdiGMP), a major regulator of biofilm formation, regulated adherence to the catheter surface in a selected clinical isolate suggesting that the cdiGMP second messenger pathway may be a target for anti-biofilm therapeutic approaches.
Wang
Xiaoda
X
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE 280, SE-171 77, Stockholm, Sweden.
Lünsdorf
Heinrich
H
Ehrén
Ingrid
I
Brauner
Annelie
A
Römling
Ute
U
D018830:Adhesins, Escherichia coli / Q000737:chemistry; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D018441:Biofilms; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D004866:Equipment Contamination; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D036781:Fimbriae Proteins / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D019081:O Antigens / Q000378:metabolism; D015290:Second Messenger Systems; D016415:Sequence Alignment; D014546:Urinary Catheterization / Q000295:instrumentation*; D014556:Urine / Q000382:microbiology
D018830:Adhesins, Escherichia coli; D019081:O Antigens; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP