title: A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse.
abstract: OBJECTIVE
  To develop an in vitro strategy to support the growth of early-stage follicles and produce mature oocytes competent for fertilization.
  
  
  METHODS
  Whole ovaries from 8-day-old mice were cultured for 4 days, and then secondary follicles were isolated and cultured for 12 days in a three-dimensional alginate or fibrin-alginate (FA) hydrogel matrix.
  
  
  METHODS
  University-affiliated laboratory.
  
  
  METHODS
  Mice.
  
  
  METHODS
  None.
  
  
  METHODS
  Histologic evaluation of follicle development, steroid hormone production, and rates of oocyte maturation, oocyte fertilization, and embryo formation.
  
  
  RESULTS
  Culture of 8-day-old mouse ovaries for 4 days resulted in transition of the follicle population from primordial and primary follicles to secondary follicles, similar to that seen in a 12-day-old ovary. Isolated secondary follicles cultured for 12 days showed larger increases in oocyte diameter and more frequent antrum formation and theca cell differentiation in the FA-hydrogel matrix compared with the alginate matrix. Steroid hormone secretion patterns were consistent with the changes in follicle morphology and cell differentiation observed in the cultured follicles. Compared with oocytes from alginate follicle cultures, a greater number of oocytes retrieved from the FA-based follicle cultures progressed to metaphase I, reached metaphase II, and could be fertilized and cleaved to two-cell embryos. The organ culture plus FA-hydrogel follicle culture strategy produced a very high rate of oocyte progression to metaphase II (88 +/- 8.7% [mean +/- SEM]) and formation of two-cell embryos (54 +/- 4%).
  
  
  CONCLUSIONS
  A strategy combining whole ovary culture of early-stage follicles and subsequent FA hydrogel in vitro follicle culture produced a high percentage of oocytes competent for fertilization; this might provide new options for fertility preservation in women and prepubertal girls facing fertility-threatening diseases or treatments.
authors.affiliation: Center for Reproductive Research, Northwestern University, Evanston, Illinois, USA.
authors.forename: Shi Ying
authors.initials: SY
authors.lastname: Jin
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Ariella
authors.initials: A
authors.lastname: Shikanov
authors.forename: Lonnie D
authors.initials: LD
authors.lastname: Shea
authors.forename: Teresa K
authors.initials: TK
authors.lastname: Woodruff
mesh_terms: D000464:Alginates / Q000494:pharmacology; D000818:Animals; D003470:Culture Media; D005260:Female; D005307:Fertilization in Vitro; D005337:Fibrin / Q000494:pharmacology; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000166:cytology / Q000254:growth & development; D009924:Organ Culture Techniques / Q000379:methods*; D006080:Ovarian Follicle / Q000254:growth & development; D010053:Ovary / Q000166:cytology; D046509:Tissue Culture Techniques
chemical_list: D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005337:Fibrin