title: Changes in gene and protein expression in magnetic field-treated human glioma cells.
abstract: Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 muT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 muT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.
authors.affiliation: National Institute for Occupational Safety and Health (NIOSH), Cincinnati, Ohio, 45226.
authors.forename: R E
authors.initials: RE
authors.lastname: Savage
authors.forename: M H
authors.initials: MH
authors.lastname: Kanitz
authors.forename: W G
authors.initials: WG
authors.lastname: Lotz
authors.forename: D
authors.initials: D
authors.lastname: Conover
authors.forename: E M
authors.initials: EM
authors.lastname: Hennessey
authors.forename: W H
authors.initials: WH
authors.lastname: Hanneman
authors.forename: F A
authors.initials: FA
authors.lastname: Witzmann