title: Potato virus X as a novel platform for potential biomedical applications.
abstract: We demonstrate that nanoparticles formed from the rod-shaped plant virus Potato virus X (PVX) can serve as a novel platform for biomedical applications. Bioconjugation protocols including amine modification and "click" chemistry allowed the efficient functionalization of PVX with biotins, dyes, and PEGs. Fluorescent-labeled and PEGylated PVX particles revealed that different fluorescent labels have a profound effect on PVX-cell interactions. Applying bioconjugation chemistries to PVX opens the door for chemical functionalization with targeting and therapeutic molecules.
authors.affiliation: Department of Cell Biology, Center for Integrative Molecular Biosciences, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
authors.forename: Marianne E
authors.initials: ME
authors.lastname: Mertens
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Taurog
authors.forename: John E
authors.initials: JE
authors.lastname: Johnson
authors.forename: Ulrich
authors.initials: U
authors.lastname: Commandeur
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.forename: Marianne
authors.initials: M
authors.lastname: Manchester
mesh_terms: D001709:Biotechnology / Q000379:methods; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D011092:Polyethylene Glycols / Q000737:chemistry; D017863:Potexvirus / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012984:Software; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes; D012367:RNA, Viral; D011092:Polyethylene Glycols; D001710:Biotin