The putative Bacillus subtilis L,D-transpeptidase YciB is a lipoprotein that localizes to the cell poles in a divisome-dependent manner.
Cell wall synthesis in bacteria is spatially organized by cytoskeletal structures. Common to all cell wall-bearing bacteria, the cytokinetic machinery localizes the cell wall synthesis to the site of septation. Recently, MinJ, a new component of the cytokinetic machinery, or divisome, of Bacillus subtilis has been described. MinJ is part of the division site selection system but also essential for correct assembly of the divisome. Here, I used the isolated PDZ domain of MinJ for co-elution experiments. One of the proteins that co-eluted was the so far uncharacterized, putative L,D-transpeptidase protein YciB. Evidence is shown that YciB localizes to the cell poles. YciB localization depends on the existence of a mature divisome, suggesting that L,D-transpeptidases are, like penicillin-binding proteins, part of the divisome.
Bramkamp
Marc
M
Institute for Biochemistry, University of Cologne, Zülpicher Strasse 47, Cologne, Germany. marc.bramkamp@uni-koeln.de
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D001419:Bacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity / Q000235:genetics; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D010458:Peptidyl Transferases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D018797:Cell Cycle Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D010458:Peptidyl Transferases; D000251:Adenosine Triphosphatases