title: Prognostic Significance and Clinicopathological Associations of COX-2 SNP in Patients with Nonsmall Cell Lung Cancer.
abstract: Background. To further improve the screening, diagnosis, and therapy of patients with nonsmall cell lung cancer (NSCLC) additional diagnostic tools are urgently needed. Gene expression of Cyclooxygenase-2 (COX-2) has been linked to prognosis in patients with NSCLC. The role of the COX-2 926G>C Single Nucleotide Polymorphism (SNP) in patients with NSCLC remains unclear. The aim of this study was to investigate the potential of the COX-2 926G>C SNP as a molecular marker in this disease. Methods. COX-2 926G>C SNP was analyzed in surgically resected tumor tissue of 85 patients with NSCLC using a PCR-based RFLP technique. Results. The COX-2 926G>C SNP genotypes were detected with the following frequencies: GG n = 62 (73%), GC n = 20 (23%), CC n = 3 (4%). There were no associations between COX-2 SNP genotype and histology, grading or gender detectable. COX-2 SNP was significantly associated with tumor stage (P = .032) and lymph node status (P = .016, Chi-square test). With a median followup of 85.9 months, the median survival was 59.7 months. There were no associations seen between the COX-2 SNP genotype and patients prognosis. Conclusions. The COX-2 926G>C SNP is detectable at a high frequency in patients with NSCLC. The COX-2 926G>C SNP genotype is not a prognostic molecular marker in this disease. However, patients with the GC or CC genotype seem more susceptible to lymph node metastases and higher tumor stage than patients with the GG genotype. The results suggest COX-2 926G>C SNP as a molecular marker for lymph node involvement in this disease.
authors.affiliation: Department of General, Visceral and Tumor Surgery, University of Cologne, 50931 Cologne, Germany.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Grimminger
authors.forename: Jan
authors.initials: J
authors.lastname: Stöhlmacher
authors.forename: Daniel
authors.initials: D
authors.lastname: Vallböhmer
authors.forename: Paul M
authors.initials: PM
authors.lastname: Schneider
authors.forename: Arnulf H
authors.initials: AH
authors.lastname: Hölscher
authors.forename: Ralf
authors.initials: R
authors.lastname: Metzger
authors.forename: Peter V
authors.initials: PV
authors.lastname: Danenberg
authors.forename: Jan
authors.initials: J
authors.lastname: Brabender