Relation of the expression of transcriptional factor TFAP2B to that of adipokines in subcutaneous and omental adipose tissues.
To determine the potential role of the transcriptional factor-activating enhancer-binding protein-2beta (TFAP2B) in the regulation of expression of adipokines, adiponectin, leptin, and interleukin-6 (IL-6) in vivo, we quantified the mRNA expression levels of these adipokines and TFAP2B in visceral (omental) and abdominal subcutaneous adipose tissues of 66 individuals with variable degree of adiposity and studied their correlations with BMI and their plasma concentrations. We found that BMI correlated negatively with plasma adiponectin levels and positively with those of leptin. Adiponection mRNA expression in subcutaneous fat correlated negatively with BMI, whereas leptin mRNA levels in the omentum correlated with plasma leptin levels and BMI. In contrast, IL-6 mRNA levels in subcutaneous and omental fat did not correlate with BMI. IL-6 mRNA levels in the omental fat correlated with plasma IL-6 levels. Whereas TFAP2B mRNA expression did not correlate with BMI, it correlated negatively with adiponectin expression in the subcutaneous adipose tissue. Furthermore, TFAP2B mRNA expression correlated negatively with leptin and positively with IL-6 expression in both subcutaneous and omental adipose tissues. These relationships are consistent with our in vitro observations and indicate that TFAP2B seems to regulate the expression of various adipokines in vivo.
Ugi
Satoshi
S
Department of Medicine, Shiga University of Medical Science, Shiga, Japan. sugi@belle.shiga-med.ac.jp
Nishio
Yoshihiko
Y
Yamamoto
Hiroshi
H
Ikeda
Kazuhiro
K
Kobayashi
Masaaki
M
Tsukada
Shuichi
S
Kondo
Motoyuki
M
Morino
Katsutaro
K
Obata
Toshiyuki
T
Yoshizaki
Takeshi
T
Uzu
Takashi
T
Naitoh
Hiroyuki
H
Tani
Toru
T
Maeda
Shiro
S
Kashiwagi
Atsunori
A
Maegawa
Hiroshi
H
D050153:Abdominal Fat / Q000502:physiology; D052242:Adiponectin / Q000097:blood / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D020738:Leptin / Q000097:blood / Q000235:genetics*; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008875:Middle Aged; D009852:Omentum / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D050151:Subcutaneous Fat / Q000502:physiology*; D050656:Transcription Factor AP-2 / Q000235:genetics* / Q000378:metabolism
C499918:ADIPOQ protein, human; D052242:Adiponectin; C508600:IL6 protein, human; D015850:Interleukin-6; D020738:Leptin; D012333:RNA, Messenger; C493677:TFAP2B protein, human; D050656:Transcription Factor AP-2