title: The allogeneic but not syngeneic dendritic cells effectively generated regulatory T cells from total CD4+ population without exogenous cytokines.
abstract: Dendritic cells (DC) play a critical role in both the expansion of natural regulatory T cells (nTreg) and conversion of induced Treg (iTreg) from their precursors. In the present study, we evaluated the potential of DC to generate Treg from total CD4(+) population which contains both nTreg and the precursors, and found that allogeneic (allo-DC) but not syngeneic DC (syn-DC) could effectively generated Foxp3(+) Treg from total CD4(+) population in the absence of exogenous cytokines. Compared with freshly purified CD4(+) T cells, allo-DC-stimulated CD4(+) T cells showed increased percentage of CD4(+)CD25(+)Foxp3(+) Treg by 5-7-folds while syn-DC-stimulated CD4(+) T cells did not. Furthermore, we demonstrated that the significant amounts of endogenous IL-2 and TGF-beta, at least partially, contributed to the expansion of nTreg and conversion of iTreg in this cocultural system, respectively. Importantly, similar to nTreg, these allo-DC-generated Treg were capable of suppressing T cell response in vitro. Thus, our research provides a novel and efficient strategy for generation of Treg from total CD4(+) population.
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Guo
authors.forename: C
authors.initials: C
authors.lastname: Ma
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology*; D005260:Female; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology
chemical_list: D007376:Interleukin-2; D016212:Transforming Growth Factor beta