title: Proteomic profiling of the rat cerebral cortex in sleep and waking.
abstract: Transcriptomic studies have shown that hundreds of genes change their expression levels across the sleep/waking cycle, and found that waking-related and sleep-related mRNAs belong to different functional categories. Proteins, however, rather than DNA or RNA, carry out most of the cellular functions, and direct measurements of protein levels and activity are required to assess the effects of behavioral states on the overall functional state of the cell. Here we used surface-enhanced laser desorption-ionization (SELDI), followed by time-of-flight mass spectrometry, to obtain a large-scale profiling of the proteins in the rat cerebral cortex whose expression is affected by sleep, spontaneous waking, short (6 hours) and long (7 days) sleep deprivation. Each of the 94 cortical samples was profiled in duplicate on 4 different ProteinChip Array surfaces using 2 different matrix molecules. Overall, 1055 protein peaks were consistently detected in cortical samples and 15 candidate biomarkers were selected for identification based on significant changes in multiple conditions (conjunction analysis): 8 "sleep" peaks, 4 "waking" peaks, and 4 "long sleep deprivation" peaks. Four candidate biomarkers were purified and positively identified. The 3353 Da candidate sleep marker was identified as the 30 amino acid C-terminal fragment of rat histone H4. This region encompasses the osteogenic growth peptide, but a possible link between sleep and this peptide remains highly speculative. Two peaks associated with short and long sleep deprivation were identified as hemoglobin alpha1/2 and beta, respectively, while another peak associated with long sleep deprivation was identified as cytochrome C. The upregulation of hemoglobins and cytochrome C may be part of a cellular stress response triggered by even short periods of sleep loss.
authors.affiliation: Dept. of Psychiatry, University of Wisconsin-Madison, 6001 Research Park Blvd. Madison, WI 53719, USA. ccirelli@wisc.edu
authors.forename: C
authors.initials: C
authors.lastname: Cirelli
authors.forename: M
authors.initials: M
authors.lastname: Pfister-Genskow
authors.forename: D
authors.initials: D
authors.lastname: McCarthy
authors.forename: R
authors.initials: R
authors.lastname: Woodbury
authors.forename: G
authors.initials: G
authors.lastname: Tononi
mesh_terms: D000818:Animals; D015415:Biomarkers; D002540:Cerebral Cortex / Q000502:physiology*; D045304:Cytochromes c / Q000502:physiology; D004567:Electrodes, Implanted; D004569:Electroencephalography; D006454:Hemoglobins / Q000502:physiology; D006657:Histones / Q000502:physiology; D008297:Male; D040081:Protein Array Analysis; D040901:Proteomics; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000502:physiology*; D012892:Sleep Deprivation / Q000503:physiopathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014851:Wakefulness / Q000502:physiology*
chemical_list: D015415:Biomarkers; D006454:Hemoglobins; D006657:Histones; D045304:Cytochromes c