The allogeneic but not syngeneic dendritic cells effectively generated regulatory T cells from total CD4+ population without exogenous cytokines.
Dendritic cells (DC) play a critical role in both the expansion of natural regulatory T cells (nTreg) and conversion of induced Treg (iTreg) from their precursors. In the present study, we evaluated the potential of DC to generate Treg from total CD4(+) population which contains both nTreg and the precursors, and found that allogeneic (allo-DC) but not syngeneic DC (syn-DC) could effectively generated Foxp3(+) Treg from total CD4(+) population in the absence of exogenous cytokines. Compared with freshly purified CD4(+) T cells, allo-DC-stimulated CD4(+) T cells showed increased percentage of CD4(+)CD25(+)Foxp3(+) Treg by 5-7-folds while syn-DC-stimulated CD4(+) T cells did not. Furthermore, we demonstrated that the significant amounts of endogenous IL-2 and TGF-beta, at least partially, contributed to the expansion of nTreg and conversion of iTreg in this cocultural system, respectively. Importantly, similar to nTreg, these allo-DC-generated Treg were capable of suppressing T cell response in vitro. Thus, our research provides a novel and efficient strategy for generation of Treg from total CD4(+) population.
Liu
C
C
Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, China.
Wang
Q
Q
Liu
F
F
Li
H
H
Zhu
F
F
Wang
X
X
Guo
C
C
Ma
C
C
Zhang
L
L
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology*; D005260:Female; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology
D007376:Interleukin-2; D016212:Transforming Growth Factor beta