Identification of candidate genes involved in endogenous protection mechanisms against acute pancreatitis in mice.
We surveyed changes of the gene expression profile in caerulein-exposed pancreas using Affymetrix GeneChip system (39,000 genes). Up-regulation of genes coding for claudin 4, claudin 7, F11 receptor, cadherin 1, integrin beta 4, syndecan 1, heat shock proteins b1/90aa1, Serpinb6a, Serpinb6b, Serpinb9, Bax, Bak1, calpain 2, calpain 5, microtubule-associated protein 1 light chain 3 alpha, S100 calcium-binding proteins A4/A10 were found in mouse pancreas exposed to caerulein for 12h. In contrast, the anti-apoptotic gene Bcl2 was down-regulated. The functions of these genes concern tight junction formation, cell-cell/cell-matrix adhesions, stress response, protease inhibition, apoptosis, autophagy, and regulation of cytoskeletal dynamics. Caerulein-exposed pancreatic acinar cells were immunohistochemically stained for claudin 4, cadherin 1, integrin beta 4, heat shock protein b1, and Serpinb6a. In conclusion, we have newly identified a set of genes that are likely to be involved in endogenous self-protection mechanisms against acute pancreatitis.
Nakada
Shinji
S
Department of Japanese Oriental Medicine, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, Sugitani 2630, Toyama 930-0194, Japan.
Tsuneyama
Koichi
K
Kato
Ichiro
I
Tabuchi
Yoshiaki
Y
Takasaki
Ichiro
I
Furusawa
Yukihiro
Y
Kawaguchi
Hiroshi
H
Fujimoto
Makoto
M
Goto
Hirozo
H
Hikiami
Hiroaki
H
Kondo
Takashi
T
Takano
Yasuo
Y
Shimada
Yutaka
Y
D000208:Acute Disease; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001343:Autophagy / Q000235:genetics; D002108:Ceruletide / Q000633:toxicity; D015870:Gene Expression; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007365:Intercellular Junctions / Q000187:drug effects / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010195:Pancreatitis / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D053491:Proteinase Inhibitory Proteins, Secretory / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D006360:Heat-Shock Proteins; D053491:Proteinase Inhibitory Proteins, Secretory; D009418:S100 Proteins; D002108:Ceruletide