Yes-associated protein 1 regulates the stability of DeltaNp63alpha.
Regulation of DeltaNp63alpha can be achieved at the transcriptional and post-translational levels, with the latter being greatly influenced by external stimuli such as DNA damaging agents. In this present study, we demonstrated that DeltaNp63alpha physical interacts with Yes-associated protein 1 (Yap1). Ectopic expression of Yap1 decreased the stability and the half-like of DeltaNp63alpha and resulted in ubiquitin-dependent degradation of DeltaNp63alpha. Further, the cisplatin mediated degradation of DeltaNp63alpha is attenuated with the downregulation of Yap1. Thus, our results strongly suggest that Yap1 plays a role in the regulation of endogenous DeltaNp63alpha levels and is likely to contribute to the regulation of DeltaNp63alpha, in physiological conditions.
Chatterjee
Aditi
A
Department of Otolaryngology-Head and Neck Surgery, Head and Neck Cancer Research Division, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sen
Tanusree
T
Chang
Xiaofei
X
Sidransky
David
D
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D006801:Humans; D047468:Immunoprecipitation; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D055550:Protein Stability; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D000091102:YAP-Signaling Proteins
D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human