Investigation of the faecal microbiota associated with canine chronic diarrhoea.
Diarrhoea is a common problem in dogs and can result in disturbance of the normal intestinal microbiota. However, little is known about the gastrointestinal microbiota of dogs with chronic diarrhoea and controlled canine studies of dietary management are scarce. The aims of this study were to investigate the predominant faecal microbiota of chronic diarrhoea dogs and to examine the effect(s) of a fibre blend on the canine faecal microbiota. A 3-week fibre supplementation feeding study was performed in nine chronic diarrhoea and eight control dogs. Atopobium cluster, Lactobacillus-Enterococcus group and Clostridium cluster XIV were the predominant bacterial groups in all dogs. Chronic diarrhoea dogs had significantly higher Bacteroides counts at baseline and significantly lower Atopobium cluster counts following fibre supplementation compared with control dogs. Atopobium cluster levels increased significantly in control dogs, while counts of sulphate-reducing bacteria decreased significantly and Clostridium clusters I and II counts increased significantly in chronic diarrhoea dogs during fibre supplementation. Microbial profiles (detected by denaturing gradient gel electrophoresis) demonstrated interindividual variation, with greater similarity seen between the chronic diarrhoea and control dogs' profiles after fibre supplementation compared with baseline. In conclusion, fibre supplementation induced changes in the canine faecal microbiota, with greater resemblance between the microbiota of chronic diarrhoea and control dogs after this dietary modulation.
Jia
Jie
J
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, UK.
Frantz
Nolan
N
Khoo
Christina
C
Gibson
Glenn R
GR
Rastall
Robert A
RA
McCartney
Anne L
AL
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004043:Dietary Fiber; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D054892:Metagenome