Enhanced tolerance of transgenic tall fescue plants overexpressing 2-Cys peroxiredoxin against methyl viologen and heat stresses.
Plant 2-Cys peroxiredoxins (2-Cys Prx) has both peroxidase and chaperon function. We overexpressed an Arabidopsis 2-Cys Prx in transgenic tall fescue (Festuca arundinacea) plants to confer tolerance against heat and methyl viologen (MV) stress. Transgenic plants were generated by Agrobacterium-mediated genetic transformation, and integration and expression of the transgene was confirmed by Southern, northern and western blot analyses. Compared to control plants, transgenic plants had significantly less electrolyte leakage and thiobarbituric acid-reactive substances (TBARS) when exposed to heat or MV. Under heat stress (42 degrees C), transgenic plants maintained their chlorophyll fluorescence (Fv/Fm) for 24 h while control plants lost chlorophyll fluorescence very quickly. We conclude that the high levels of 2-Cys Prx proteins in transgenic plants protect leaves from oxidative damage probably due to chaperon activity.
Kim
Kyung-Hee
KH
Division of Applied Life Sciences, BK21 Program, IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Korea. [corrected]
Alam
Iftekhar
I
Lee
Ki-Won
KW
Sharmin
Shamima Akhtar
SA
Kwak
Sang-Soo
SS
Lee
Sang Yeol
SY
Lee
Byung-Hyun
BH
D000704:Analysis of Variance; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D031751:Festuca / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006358:Hot Temperature; D015227:Lipid Peroxidation; D010269:Paraquat / Q000494:pharmacology; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics; D010788:Photosynthesis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D019076:Transgenes
D029681:Arabidopsis Proteins; D017392:Thiobarbituric Acid Reactive Substances; D054464:Peroxiredoxins; D010269:Paraquat