Highly ordered transparent mesoporous TiO2 thin films: an attractive matrix for efficient immobilization and spectroelectrochemical characterization of cytochrome c.
We demonstrate remarkably fast incorporation and high loading of cytochrome c within thin films of periodically ordered nanocrystalline TiO(2) deposited on transparent electrodes. The immobilized cytochrome c is not denaturated and it can be reversibly reduced without mediator over the time scale of a few seconds as evidenced by spectroelectrochemistry.
Renault
Christophe
C
Laboratoire d'Electrochimie Moléculaire, Université Paris Diderot, UMR CNRS 7591, 15, rue Jean-Antoine de Baïf, 75205 Paris Cedex 13, France.
Balland
Véronique
V
Martinez-Ferrero
Eugenia
E
Nicole
Lionel
L
Sanchez
Clément
C
Limoges
Benoît
B
D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D016062:Porosity; D013056:Spectrophotometry, Ultraviolet; D014025:Titanium / Q000737:chemistry*
D055767:Immobilized Proteins; C009495:titanium dioxide; D045304:Cytochromes c; D014025:Titanium