title: Atypical glandular cells (AGC): ThinPrep Imaging System (TIS), manual screening (MS), and correlation with Hybrid Capture 2 (HC2) HPV DNA testing.
abstract: OBJECTIVE
  The aim of the study was to determine if the ThinPrep Imaging System (T1S) improves the positive predictive value (PPV) of atypical glandular cell (AGC) diagnosis for identifying HPV-related squamous and/or glandular lesions over manual screening (MS), and if human papilloma virus (HPV)-DNA testing improves the diagnostic yield.
  
  
  METHODS
  85 ThinPrep cervical cytology specimens with a diagnosis of AGC by TIS (n = 51) and MS (n = 34) were retrieved. The diagnoses were correlated with corresponding histologic follow-up and high risk (HR)-HPV testing results.
  
  
  RESULTS
  The PPV of AGC by TIS and MS for HPV-related squamous lesions were similar. In the MS group, more cases of glandular pathology were identified, however only three represented adenocarcinoma in-situ (AIS), and the remaining ten were endometrial carcinomas (EMCA).
  
  
  CONCLUSIONS
  TIS and MS are comparable in the detection of AGC representing squamous histology and the addition of HPV DNA testing does not differentially improve performance. Although the MS group harbored more glandular pathology, the differences in the detection of AIS were not statistically significant.
authors.affiliation: Department of Pathology and Cell Biology, New York Presbyterian Hospital, Columbia University Medical Center, New York 10032, USA.
authors.forename: Anthony N
authors.initials: AN
authors.lastname: Sireci
authors.forename: John P
authors.initials: JP
authors.lastname: Crapanzano
authors.forename: Mahesh
authors.initials: M
authors.lastname: Mansukhani
authors.forename: Thomas
authors.initials: T
authors.lastname: Wright
authors.forename: Anamaria
authors.initials: A
authors.lastname: Babiac
authors.forename: Maria
authors.initials: M
authors.lastname: Erroll
authors.forename: Madeline
authors.initials: M
authors.lastname: Vazquez
authors.forename: Anjali
authors.initials: A
authors.lastname: Saqi
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D030361:Papillomavirus Infections / Q000150:complications; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000821:virology; D011237:Predictive Value of Tests; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000821:virology
chemical_list: D004279:DNA, Viral