title: Human papillomavirus 5 and 8 E6 downregulate interleukin-8 secretion in primary human keratinocytes.
abstract: Human papillomaviruses (HPVs) of the genus Betapapillomavirus appear to be involved in the early stages of skin cancer development, since both the prevalence and viral load are higher in precancerous actinic keratoses than in skin cancers. Interleukin-8 (IL-8) is an inflammatory cytokine that serves to alert the surrounding tissue after UV-induced damage. We examined the effects of the E2, E6 and E7 proteins of HPV8 and the E6 proteins of various HPV genotypes on IL-8 secretion from primary keratinocytes. HPV5 and HPV8 E6 showed the highest downregulation of basal IL-8 secretion. HPV8 E6 also negatively modulated IL-8 mRNA expression and protein secretion upon UVB irradiation. The downregulation of IL-8 in actinic keratoses may weaken the response to UV-induced damage and thus favour the accumulation of UVB-induced mutations.
authors.affiliation: Institute of Virology, University of Cologne, Germany. baki.akguel@web.de
authors.forename: Baki
authors.initials: B
authors.lastname: Akgül
authors.forename: Nagihan
authors.initials: N
authors.lastname: Bostanci
authors.forename: Kathi
authors.initials: K
authors.lastname: Westphal
authors.forename: Ingo
authors.initials: I
authors.lastname: Nindl
authors.forename: Harshad
authors.initials: H
authors.lastname: Navsaria
authors.forename: Alan
authors.initials: A
authors.lastname: Storey
authors.forename: Herbert
authors.initials: H
authors.lastname: Pfister
mesh_terms: D000328:Adult; D015536:Down-Regulation; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D055623:Keratosis, Actinic / Q000209:etiology; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D012878:Skin Neoplasms / Q000209:etiology; D014466:Ultraviolet Rays
chemical_list: C466081:E6 protein, Human papillomavirus type 8; C487708:E6 protein, human papillomavirus type 5; D016209:Interleukin-8; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger