The same well-characterized T cell epitope SIINFEKL expressed in the context of a cytoplasmic or secreted protein in BCG induces different CD8+ T cell responses.
Mycobacterium bovis BCG is still the most widely used vaccine against tuberculosis and CD8(+) T cells play important roles in fighting infection. We investigated how well antigen is processed and presented to CD8(+) T cells using the same well-characterized CD8(+) T cell epitope SIINFEKL expressed in either a cytoplasmic (GFP-OVA) or secreted (85B-OVA) context from BCG. We report that secreted SIINFEKL from 85B-OVA BCG is presented better than cytoplasmic SIINFEKL expressed by GFP-OVA BCG.
Hulseberg
Paul D
PD
Department of Pathology and Laboratory Sciences, University of Wisconsin, Madison, WI 53706, USA. pdhulseberg@wisc.edu
Zozulya
Alla
A
Chu
Hamlet H
HH
Triccas
James A
JA
Fabry
Zsuzsanna
Z
Sandor
Matyas
M
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003593:Cytoplasm / Q000187:drug effects; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism*; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology
D001500:BCG Vaccine; D018984:Epitopes, T-Lymphocyte; C110366:OVA-8; D010446:Peptide Fragments; D011994:Recombinant Proteins; D010047:Ovalbumin