Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.
A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis.
Sankar
Surya
S
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. surya_snkr@yahoo.co.in
Harshan
Hiron M
HM
Somarajan
S R
SR
Srivastava
S K
SK
D000022:Abortion, Spontaneous; D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D003001:Cloning, Molecular; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary; D041341:Leptospira interrogans serovar canicola / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011994:Recombinant Proteins / Q000032:analysis
D000942:Antigens, Bacterial; D017931:DNA Primers; C487006:LigB-m protein, Leptospira interrogans; D011994:Recombinant Proteins