title: Increased intake of ethanol and dietary fat in galanin overexpressing mice.
abstract: Evidence suggests that the orexigenic peptide, galanin (GAL), in the hypothalamic paraventricular nucleus (PVN) has a role in stimulating the consumption of ethanol, in addition to a high-fat diet. This possibility was further examined in mutant mice that overexpress the GAL gene. Two sets of GAL-overexpressors (GALOE) compared with wild-type (WT) controls, maintained on laboratory chow and water, were trained to voluntarily drink increasing concentrations of ethanol, from 3 to 15%. In the GALOE versus WT mice, the results revealed the following: (1) a 35-40% increase in ethanol intake and ethanol preference, which was evident only at the highest (15%) ethanol concentration, in male but not female mice, and was seen with comparisons to littermate and nonlittermate WT controls, (2) a significantly larger, 60-75% increase in ethanol intake and ethanol preference after a day of food deprivation, again only in male GALOE mice, (3) no change in consumption of sucrose or quinine solutions in preference tests, and (4) a 55% increase in consumption of a fat-rich diet during a 2-h test period, in both male and female GALOE mice. These results obtained with overexpression of the GAL gene provide strong support for a physiological role of this peptide in stimulating the consumption of ethanol and a fat-rich diet. They reveal gender differences in the behavioral phenotype, which may reflect GAL's functional relationship to reproductive hormones in the stimulation of consummatory behavior.
authors.affiliation: Laboratory of Behavioral Neurobiology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Karatayev
authors.forename: Jessica
authors.initials: J
authors.lastname: Baylan
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Leibowitz
mesh_terms: D000428:Alcohol Drinking; D000818:Animals; D001835:Body Weight; D002755:Choice Behavior; D004041:Dietary Fats / Q000008:administration & dosage*; D004435:Eating; D005260:Female; D005508:Food Deprivation; D019004:Galanin / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012727:Sex Characteristics; D013649:Taste
chemical_list: D004041:Dietary Fats; D019004:Galanin