Comparable susceptibilities of human 293 cells and insect Tn-5B1-4 cells to photoactivated alpha-terthienyl.
The hope is that photoactive compounds acting as potential insecticides will have reduced environmental risk, but that is not necessarily the case. In an attempt to elucidate the risk by which photoactivated alpha-terthienyl (alpha-T) affects human health, the effects of exposure of human 293 cells and insect Tn-5B1-4 cells to photoactivated alpha-T at certain doses were characterized. Photoactivated alpha-T exhibited dose dependence of toxicity and time kinetics of phototoxic activation on the growth of 293 cells (EC(50) = 6.23 microg/mL) and Tn-5B1-4 cells (EC(50) = 3.36 microg/mL). 293 cells appeared to be anchorage-independent, inflated, and broken; Tn-5B1-4 cells showed significant necrosis. ROS productions and lipid peroxidation of 293 cells were always lower than that of Tn-5B1-4 cells in the treatments of alpha-T at the same dose. Moreover, photoactivated alpha-T caused nonselective DNA damage in 293 and Tn-5B1-4 cells at a 10 microg/mL dose and induced cell-cycle progression of 293 cells to increase apoptosis of cells and G1 arrest and decrease in S phase cell population, whereas Tn-5B1-4 cells showed S arrest accompanied by a dose-dependent decrease in G1 and G2 phase cells at a 5 microg/mL dose. These observations suggest that Tn-5B1-4 cells are more susceptible to the action of photoactivated alpha-T than 293 cells, but photoactivated alpha-T as an efficient insecticide might be a potential factor in human mutagenic progression.
Huang
Qingchun
Q
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China. qchuang@ecust.edu.cn
Liu
Yang
Y
Zhan
Taisong
T
Deng
Yunfei
Y
He
Yuan
Y
D000330:Aedes; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004249:DNA Damage; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D007700:Kinetics; D008315:Malondialdehyde / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013876:Thiophenes / Q000494:pharmacology*
D007306:Insecticides; D017382:Reactive Oxygen Species; D013876:Thiophenes; C019101:alpha-terthienyl; D008315:Malondialdehyde