title: Spectrophotometric determination of copper(II) with o-bromophenylfluorone.
abstract: A simple, reliable and sensitive spectrophotometric method for the determination of copper(II) was established with o-bromophenylfluorone (OBPF), a novel chemical probe, in the presence of poly(N-vinylpyrrolidone). Beer's law was obeyed in the range of 8-160 ng ml(-1), with an apparent molar absorptivity at 570 nm, the relative standard deviation being 3.64 x 10(5) dm3 mol(-1) cm(-1) and 0.72% (n = 5). This method was applied to the recovery tests of copper(II) in human urine, bovine serum and tap water; the results were satisfactory.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. yamaguti@gly.oups.ac.jp
authors.forename: Takako
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Megumi
authors.initials: M
authors.lastname: Samma
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kamino
authors.forename: Momoka
authors.initials: M
authors.lastname: Matsushita
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D003300:Copper / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans; D010636:Phenols / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: C547578:2-bromophenylfluorone; D004396:Coloring Agents; D005452:Fluoresceins; D010636:Phenols; D014867:Water; D003300:Copper