title: A simple and rapid method of bacterial transformation.
abstract: Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar.
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. wilharmg@rki.de
authors.forename: Gottfried
authors.initials: G
authors.lastname: Wilharm
authors.forename: Daniela
authors.initials: D
authors.lastname: Lepka
authors.forename: Franziska
authors.initials: F
authors.lastname: Faber
authors.forename: Julia
authors.initials: J
authors.lastname: Hofmann
authors.forename: Tobias
authors.initials: T
authors.lastname: Kerrinnes
authors.forename: Evelyn
authors.initials: E
authors.lastname: Skiebe
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D017633:Magnesium Silicates / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry; D014169:Transformation, Bacterial; D015008:Yersinia enterocolitica / Q000235:genetics
chemical_list: D017633:Magnesium Silicates; C001671:magnesium trisilicate