title: Ectopic bone formation from mandibular osteoblasts cultured in a novel human serum-derived albumin scaffold.
abstract: The aim of this study was to evaluate the ectopic bone formation using a novel serum-derived albumin scaffold and cultured human mandibular osteoblasts in nude mice. Osteoblasts were cultured with 10% human serum and plated in a novel spongy noncalcified protein scaffold prepared with plasmatic albumin crossed with a glutaraldehyde type agent. Hematoxylin-eosin staining revealed a bone-like extracellular matrix and in vitro mineralization was confirmed by von Kossa staining. Histological and immunohistochemical evaluation showed progression of mineralization in vivo. These results suggest the clinical feasibility of alveolar cells and albumin scaffold as a good alternative for bone regeneration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Cabueñes Hospital Gijón, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Gallego
authors.forename: Luis
authors.initials: L
authors.lastname: Junquera
authors.forename: Alvaro
authors.initials: A
authors.lastname: Meana
authors.forename: Mariá
authors.initials: M
authors.lastname: Alvarez-Viejo
authors.forename: Manuel
authors.initials: M
authors.lastname: Fresno
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D002828:Choristoma / Q000473:pathology; D006801:Humans; D008297:Male; D008334:Mandible / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D012709:Serum Albumin; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D012709:Serum Albumin