title: Reduced expression of cenp-e in human hepatocellular carcinoma.
abstract: BACKGROUND
  CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2).
  
  
  METHODS
  We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation in LO2 cells. we knocked down CENP-E using shRNA expressing vector and then count the aneuploid in LO2 cells using chromosomal counts assay.
  
  
  RESULTS
  We found that both CENP-E mRNA and protein levels were significantly reduced in HCC tissues and HepG2 cells compared with para-cancerous tissues and LO2 cells, respectively. A significantly-increased proportion of aneuploid in these down-knocked LO2 cells compared with those treated with control shRNA vector.
  
  
  CONCLUSIONS
  Together with other results, these results reveal that CENP-E expression was reduced in human HCC tissue, and low CENP-E expression result in aneuploidy in LO2 cells.
authors.affiliation: The key laboratory of laboratory medical diagnostics, ministry of education; the faculty of laboratory medicine, Chongqing Medical University, Chongqing PR China. redjacky502@yahoo.com.cn
authors.forename: Zijie
authors.initials: Z
authors.lastname: Liu
authors.forename: Kang
authors.initials: K
authors.lastname: Ling
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Suyan
authors.initials: S
authors.lastname: Li
authors.forename: Qiong
authors.initials: Q
authors.lastname: Si
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Weng
mesh_terms: D000782:Aneuploidy; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; C068688:centromere protein E