title: Mesenchymal stem cells modified with stromal cell-derived factor 1 alpha improve cardiac remodeling via paracrine activation of hepatocyte growth factor in a rat model of myocardial infarction.
abstract: Mesenchymal stem cells (MSCs) are a promising source for cell-based treatment of myocardial infarction (MI), but existing strategies are restricted by low cell survival and engraftment. We examined whether SDF-1 transfection improve MSC viability and paracrine action in infarcted hearts. We found SDF-1-modified MSCs effectively expressed SDF-1 for at least 21 days after exposure to hypoxia. The apoptosis of Ad-SDF-1-MSCs was 42% of that seen in Ad-EGFP-MSCs and 53% of untreated MSCs. In the infarcted hearts, the number of DAPI-labeling cells in the Ad-SDF-1-MSC group was 5-fold that in the Ad-EGFP-MSC group. Importantly, expression of antifibrotic factor, HGF, was detected in cultured MSCs, and HGF expression levels were higher in Ad-SDF-MSC-treated hearts, compared with Ad-EGFP-MSC or control hearts. Compared with the control group, Ad-SDF-MSC transplantation significantly decreased the expression of collagens I and III and matrix metalloproteinase 2 and 9, but heart function was improved in d-SDF-MSC-treated animals. In conclusion, SDF-1-modified MSCs enhanced the tolerance of engrafted MSCs to hypoxic injury in vitro and improved their viability in infarcted hearts, thus helping preserve the contractile function and attenuate left ventricle (LV) remodeling, and this may be at least partly mediated by enhanced paracrine signaling from MSCs via antifibrotic factors such as HGF.
authors.affiliation: Institute of Clinical Medicine, Renmin Hospital, Yunyang Medical College, Shiyan, Hubei, 442000, People's Republic of China. tangjm416@163.com
authors.forename: Junming
authors.initials: J
authors.lastname: Tang
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.forename: Linyun
authors.initials: L
authors.lastname: Guo
authors.forename: Xia
authors.initials: X
authors.lastname: Kong
authors.forename: Jianye
authors.initials: J
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yongzhang
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D019899:Paracrine Communication / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection
chemical_list: D054377:Chemokine CXCL12; D017228:Hepatocyte Growth Factor