title: Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores.
abstract: A straightforward synthesis of an anthrose-containing trisaccharide derived from Bacillus anthracis was achieved. Antibodies raised against this hapten provide a highly sensitive enzyme immunoassay with a detection limit of 8.5 pmol mL(-1). By investigating the specificity of the antibodies obtained using different mono-, di- and trisaccharide synthetic analogues, we demonstrated that the epitope was mainly made up of the methyl group at C-5, the butamido group at C-4 and the hydroxyl at C-3 of the anthrose unit, the other parts of the trisaccharide appearing little involved in the recognition.
authors.affiliation: Laboratoire des Glucides, UMR-CNRS 6219, Institut de Chimie de Picardie, Université de Picardie Jules Verne, 33 rue St Leu, F-80039, Amiens, France.
authors.forename: Sandrine G Y
authors.initials: SG
authors.lastname: Dhénin
authors.forename: Vincent
authors.initials: V
authors.lastname: Moreau
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Nevers
authors.forename: Christophe
authors.initials: C
authors.lastname: Créminon
authors.forename: Florence
authors.initials: F
authors.lastname: Djedaïni-Pilard
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000276:immunology*; D000939:Epitopes; D007124:Immunoenzyme Techniques / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology; D014312:Trisaccharides / Q000032:analysis*
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D014312:Trisaccharides