Modeling withdrawal syndrome in zebrafish.
The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscience research. Zebrafish behavior is robustly affected by environmental and pharmacological manipulations, and can be examined using exploration-based paradigms, paralleled by analysis of endocrine (cortisol) stress responses. Discontinuation of various psychotropic drugs evokes withdrawal in both humans and rodents, characterized by increased anxiety. Sensitivity of zebrafish to drugs of abuse has been recently reported in the literature. Here we examine the effects of ethanol, diazepam, morphine and caffeine withdrawal on zebrafish behavior. Overall, discontinuation of ethanol, diazepam and morphine produced anxiogenic-like behavioral or endocrine responses, demonstrating the utility of zebrafish in translational research of withdrawal syndrome.
Cachat
Jonathan
J
Department of Pharmacology and Neuroscience Program, Tulane University Medical School, New Orleans, LA 70112, USA.
Canavello
Peter
P
Elegante
Marco
M
Bartels
Brett
B
Hart
Peter
P
Bergner
Carisa
C
Egan
Rupert
R
Duncan
Ashley
A
Tien
David
D
Chung
Amanda
A
Wong
Keith
K
Goodspeed
Jason
J
Tan
Julia
J
Grimes
Chelsea
C
Elkhayat
Salem
S
Suciu
Christopher
C
Rosenberg
Michael
M
Chung
Kyung Min
KM
Kadri
Ferdous
F
Roy
Sudipta
S
Gaikwad
Siddharth
S
Stewart
Adam
A
Zapolsky
Ivan
I
Gilder
Thomas
T
Mohnot
Sopan
S
Beeson
Esther
E
Amri
Hakima
H
Zukowska
Zofia
Z
Soignier
R Denis
RD
Kalueff
Allan V
AV
D000818:Animals; D001007:Anxiety / Q000209:etiology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002110:Caffeine / Q000009:adverse effects; D003975:Diazepam / Q000009:adverse effects; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects; D005106:Exploratory Behavior; D005260:Female; D006854:Hydrocortisone; D008297:Male; D009020:Morphine / Q000009:adverse effects; D013375:Substance Withdrawal Syndrome / Q000097:blood / Q000150:complications* / Q000209:etiology*; D013997:Time Factors; D015027:Zebrafish
D002110:Caffeine; D000431:Ethanol; D009020:Morphine; D003975:Diazepam; D006854:Hydrocortisone