title: A soluble form of P74 can act as a per os infectivity factor to the Autographa californica multiple nucleopolyhedrovirus.
abstract: The baculovirus occlusion-derived virion (ODV) is required to spread virus infection among insect hosts via the per os route. The Autographa californica multicapsid nucleopolyhedrovirus P74 protein is an ODV envelope protein that is essential for ODVs to be infectious. P74 is anchored in the ODV envelope by a C-terminal transmembrane anchor domain and is N-terminally exposed on the ODV surface. In the present study, a series of N-terminal and C-terminal truncation mutants of P74 were evaluated for their ability to rescue per os infectivity of the P74-null virus, AcLP4. It was discovered that a P74 truncation mutant lacking the C-terminal transmembrane anchor domain of P74 was able to rescue per os infection. This result shows that a soluble form of P74 retains per os infectivity factor function and suggests that P74 may be complexed with other proteins in the ODV envelope.
authors.affiliation: Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Slack
authors.forename: Susan D
authors.initials: SD
authors.lastname: Lawrence
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
authors.forename: Basil M
authors.initials: BM
authors.lastname: Arif
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins; D007915:Lepidoptera / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D017434:Protein Structure, Tertiary; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000502:physiology*
chemical_list: C109481:Ac-p74 protein, Autographa californica nucleopolyhedrovirus; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins