Facing the challenge of biosample imaging by FTIR with a synchrotron radiation source.
Fourier-transform infrared (FTIR) synchrotron radiation (SR) microspectroscopy is a powerful molecular probe of biological samples at cellular resolution (<10 microm). As the brilliance of SR is 100-1000 times higher than that of a conventional Globar source, FTIR microscopes are now available in almost all advanced SR facilities around the world. However, in spite of this superior performance, the expected advances in IR SR microscopy have not yet been realised, particularly with regard to bio-analytical studies of single cells and soft tissues. In recent decades solid-state array detectors have revolutionized the fields of molecular spectroscopy and chemical imaging, and now new IR focal plane array detectors implemented at ultra-bright SR facilities will extend the performance and overcome the existing limitations, possibly allowing IR SR instrumentation to achieve the highest sensitivity and resolution of molecular imaging. The impact of IR imaging on large tissue area and the complexity of the analysis are discussed. In view of the high brilliance of SR sources, a comparison of published microscope images is given. Finally, it is briefly outlined how an optimized combination of IR instrumentation and SR optical systems could reach the expected advantages of a SR-based FTIR imaging system.
Petibois
Cyril
C
Université de Bordeaux 2, CNRS UMR 5248 CBMN, B8 Avenue des facultés, F-33405 Talence Cedex, France. cyril.petibois@u-bordeaux2.fr
Piccinini
Massimo
M
Guidi
Mariangela Cestelli
MC
Marcelli
Augusto
A
D001704:Biopolymers / Q000032:analysis*; D004867:Equipment Design; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods*; D017356:Synchrotrons / Q000295:instrumentation*; D013673:Technology Assessment, Biomedical
D001704:Biopolymers