Comparison of tissue microarray and full section in immunohistochemistry of gastrointestinal stromal tumors.
The aim of the present study was to compare the efficiency of tissue microarray (TMA) results using immunohistochemistry markers applied to a variety of core sizes and full sections of gastrointestinal stromal tumors (GIST) using performance measures such as core loss rate and concordance rate. Six primary antibody markers (c-Kit, CD34, smooth muscle actin (SMA), S-100, p53, and Ki-67) were used with the TMA technique to analyze 0.6 mm, 2 mm, and 3 mm punch cores of GIST samples from 67 patients. No statistical association was found between core size and loss rate (P= 0.512). TMA results for the 0.6 mm, 2 mm, and 3 mm core showed that all core sizes could statistically significantly reflect full sections with regard to c-Kit, SMA, and S-100 antibodies, but that the 3 mm core section was the most representative except for CD34. With regard to p53 and Ki-67 staining, the 0.6 mm core section was not representative, but the 2 mm and 3 mm core sections could statistically significantly represent full section results. Among them, the 3 mm core section was more accurate than the 2 mm core section. Use of a single 3 mm core size in TMA is suitable for evaluating large numbers of protein and nuclear stains with regard to immunohistochemistry for GIST.
Kwon
Mi Jung
MJ
Departments of Pathology, Kangdong Sacred-Heart Hospital, Hallym University Medical College, Seoul.
Nam
Eun Sook
ES
Cho
Seong Jin
SJ
Park
Hye Rim
HR
Shin
Hyung Sik
HS
Park
Jun Ho
JH
Park
Chan Heun
CH
Lee
Won Jae
WJ
D000199:Actins / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis*; D046152:Gastrointestinal Stromal Tumors / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009418:S100 Proteins / Q000096:biosynthesis; D019089:Stem Cell Factor / Q000096:biosynthesis; D046888:Tissue Array Analysis / Q000379:methods*
D000199:Actins; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D009418:S100 Proteins; D019089:Stem Cell Factor