Distinct ONE-GC transduction modes and motifs of the odorants: Uroguanylin and CO(2).
In a subset of the olfactory sensory neurons ONE-GC($) membrane guanylate cyclase is a central component of two odorant-dependent cyclic GMP signaling pathways. These odorants are uroguanylin and CO(2). The present study was designed to decipher the biochemical and molecular differences between these two odorant signaling mechanisms. The study shows (1) in contrast to uroguanylin, CO(2) transduction mechanism is Ca(2+)-independent. (2) CO(2) transduction site, like that of uroguanylin-neurocalcin delta, resides in the core catalytic domain, aa 880-1028, of ONE-GC. (3) The site, however, does not overlap the signature neurocalcin delta signal transduction domain, (908)LSEPIE(913). Finally, (4) this study negates the prevailing concept that CO(2) uniquely signals ONE-GC activity (Sun et al. [19]; Guo et al. [21]). It demonstrates that it also signals the activation of photoreceptor membrane guanylate cyclase ROS-GC1. These results show an additional new transduction mechanism of the membrane guanylate cyclases and broaden our understanding of the molecular mechanisms by which different odorants using a single guanylate cyclase can regulate diverse cyclic GMP signaling pathways.
Duda
Teresa
T
Research Divisions of Biochemistry and Molecular Biology, The Unit of Regulatory and Molecular Biology, Salus University, Elkins Park, PA 19027, USA. tduda@salus.edu
Sharma
Rameshwar K
RK
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D019556:COS Cells; D002245:Carbon Dioxide / Q000378:metabolism*; D020134:Catalytic Domain / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology; D002522:Chlorocebus aethiops; D006152:Cyclic GMP / Q000378:metabolism; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D045265:Natriuretic Peptides / Q000378:metabolism*; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000201:enzymology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
D001639:Bicarbonates; D045265:Natriuretic Peptides; D011956:Receptors, Cell Surface; C110715:guanylate cyclase 1; D002245:Carbon Dioxide; C084362:uroguanylin; D006162:Guanylate Cyclase; D006152:Cyclic GMP