title: Proliferative response of microglia and macrophages in the adult mouse eye after optic nerve lesion.
abstract: OBJECTIVE
  The purpose of this in vivo study was to evaluate the proliferative response of immunologic cells during the acute phase after optic nerve (ON) lesion in the neural retina and the ciliary body (CB) in the adult mouse.
  
  
  METHODS
  The number of cells obtained 5 to 10 days after ON crush was compared with that counted after intraorbital ON transection. In addition, after ON crush, the time course of in situ proliferating Ki67(+) microglia and macrophages was analyzed from 6 hours up to 10 days.
  
  
  RESULTS
  The number of BrdU(+)F4/80(+) retinal microglia and ciliary macrophages increased over time, reaching the peak number 10 days after ON lesion. In the retina, both ON lesion types resulted in a similar number of BrdU(+)F4/80(+) microglia. Approximately 85% of all BrdU(+) cells were identified as F4/80(+) microglia. However, this cell population represented only 30% of all F4/80(+) microglia. The peak of microglial in situ proliferation was found 2 days after ON crush. In the CB, both ON lesion types induced a significant increase in the number of BrdU(+)F4/80(+) macrophages. Of interest, the number of cells after ON transection further increased over time, whereas those after ON crush did not.
  
  
  CONCLUSIONS
  ON lesion significantly increased proliferation of F4/80(+) immunologic cells in both the retina and CB. Although no significant differences in cellular response were observed in the retina between both lesion types, ON transection had a more pronounced effect on ciliary macrophages than did ON crush. Therefore, both regions seem not to act in concert during the acute phase after ON lesion.
authors.affiliation: Hans Berger Department of Neurology, Friedrich-Schiller University, Jena, Germany. stefanie.wohl@med.uni-jena.de
authors.forename: Stefanie G
authors.initials: SG
authors.lastname: Wohl
authors.forename: Christian W
authors.initials: CW
authors.lastname: Schmeer
authors.forename: Otto W
authors.initials: OW
authors.lastname: Witte
authors.forename: Stefan
authors.initials: S
authors.lastname: Isenmann
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D019771:Axotomy; D002452:Cell Count; D049109:Cell Proliferation; D002924:Ciliary Body / Q000166:cytology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000378:metabolism; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000166:cytology*; D020221:Optic Nerve Injuries / Q000473:pathology; D055351:Retinal Neurons / Q000166:cytology; D016707:Tissue Fixation
chemical_list: D000943:Antigens, Differentiation; D019394:Ki-67 Antigen; C036763:monocyte-macrophage differentiation antigen