title: Cellular distribution of chromogranin A in excitatory, inhibitory, aminergic and peptidergic neurons of the rodent central nervous system.
abstract: Immunoreactivity for both processed and unprocessed forms of chromogranin A (CGA) was examined, using an antibody recognizing the WE14 epitope, among terminal fields and cell bodies of anatomically defined GABAergic, glutamatergic, cholinergic, catecholaminergic, and peptidergic cell groups in the rodent central nervous system. CGA is ubiquitous within neuronal cell bodies, with no obvious anatomical or chemically-coded subdivision of the nervous system in which CGA is not expressed in most neurons. CGA expression is essentially absent from catecholaminergic terminal fields in the CNS, suggesting a relative paucity of large dense-core vesicles in CNS compared to peripheral catecholaminergic neurons. Extensive synaptic co-localization with classical transmitter markers is not observed even in areas such as amygdala, where CGA fibers are numerous, suggesting preferential segregation of CGA to peptidergic terminals in CNS. Localization of CGA in dendrites in some areas of CNS may indicate its involvement in regulation of dendritic release mechanisms. Finally, the ubiquitous presence of CGA in neuronal cell somata, especially pronounced in GABAergic neurons, suggests a second non-secretory vesicle-associated function for CGA in CNS. We propose that CGA may function in the CNS as a prohormone and granulogenic factor in some terminal fields, but also possesses as-yet unknown unique cellular functions within neuronal somata and dendrites.
authors.affiliation: Institute of Anatomy and Cell Biology, University of Marburg, Marburg, Germany.
authors.forename: M K-H
authors.initials: MK
authors.lastname: Schafer
authors.forename: S K
authors.initials: SK
authors.lastname: Mahata
authors.forename: N
authors.initials: N
authors.lastname: Stroth
authors.forename: L E
authors.initials: LE
authors.lastname: Eiden
authors.forename: E
authors.initials: E
authors.lastname: Weihe
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D053379:Chromogranin A / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009474:Neurons / Q000378:metabolism*
chemical_list: D053379:Chromogranin A