Fluorescence methods for evaluating lipoplex-mediated gene delivery.
The biological activity of cationic liposome/DNA complexes ("lipoplexes") is strongly dependent on their ability to protect DNA and to interact with cells, including binding to the cell surface, internalization via endocytosis and cytoplasmic delivery of the DNA. In this chapter, we describe a number of methods and procedures to study these processes, based on the use of fluorescent probes.
Faneca
Henrique
H
Center for Neuroscience and Cell Biology, University of Coimbra, Portugal.
Düzgüneş
Nejat
N
Lima
Maria C
MC
D000230:Adenocarcinoma / Q000235:genetics; D000818:Animals; D002412:Cations / Q000032:analysis / Q000378:metabolism; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004705:Endocytosis; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000032:analysis* / Q000378:metabolism*; D015674:Mammary Neoplasms, Animal / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal / Q000379:methods*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA