title: Membrane association of mitochondrial DNA facilitates base excision repair in mammalian mitochondria.
abstract: Mitochondrial DNA encodes a set of 13 polypeptides and is subjected to constant oxidative stress due to ROS production within the organelle. It has been shown that DNA repair in the mitochondrion proceeds through both short- and long-patch base excision repair (BER). In the present article, we have used the natural competence of mammalian mitochondria to import DNA and study the sub-mitochondrial localization of the repair system in organello. Results demonstrate that sequences corresponding to the mtDNA non-coding region interact with the inner membrane in a rapid and saturable fashion. We show that uracil containing import substrates are taken into the mitochondrion and are used as templates for damage driven DNA synthesis. After further sub-fractionation, we show that the length of the repair synthesis patch differs in the soluble and the particulate fraction. Bona fide long patch BER synthesis occurs on the DNA associated with the particulate fraction, whereas a nick driven DNA synthesis occurs when the uracil containing DNA accesses the soluble fraction. Our results suggest that coordinate interactions of the different partners needed for BER is only found at sites where the DNA is associated with the membrane.
authors.affiliation: Mitochondrial Research Group, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
authors.forename: Pierre
authors.initials: P
authors.lastname: Boesch
authors.forename: Noha
authors.initials: N
authors.lastname: Ibrahim
authors.forename: André
authors.initials: A
authors.lastname: Dietrich
authors.forename: Robert N
authors.initials: RN
authors.lastname: Lightowlers
mesh_terms: D000818:Animals; D001692:Biological Transport; D004249:DNA Damage; D015342:DNA Probes; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013698:Templates, Genetic; D014498:Uracil / Q000032:analysis
chemical_list: D015342:DNA Probes; D004272:DNA, Mitochondrial; D014498:Uracil