title: Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes.
abstract: Cell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.
authors.affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium.
authors.forename: Joanna
authors.initials: J
authors.lastname: Boruc
authors.forename: Evelien
authors.initials: E
authors.lastname: Mylle
authors.forename: Maria
authors.initials: M
authors.lastname: Duda
authors.forename: Rebecca
authors.initials: R
authors.lastname: De Clercq
authors.forename: Stephane
authors.initials: S
authors.lastname: Rombauts
authors.forename: Danny
authors.initials: D
authors.lastname: Geelen
authors.forename: Pierre
authors.initials: P
authors.lastname: Hilson
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Daniel
authors.initials: D
authors.lastname: Van Damme
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D032461:Chromosomes, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D049452:Green Fluorescent Proteins