title: Designed surface with tunable IgG density as an in vitro model for immune complex mediated stimulation of leukocytes.
abstract: We present the design of an in vitro model for immune-complex-mediated stimulation of leukocytes and its functional characteristics with respect to monocyte adhesion. The model was based on the orientation-controlled immobilization of a humanized IgG1 monoclonal antibody (rituximab) via its interaction with a biotinylated peptide epitope derived from the CD20 marker. The peptide was linked to neutravidin covalently attached to a mixed self-assembled monolayer of carboxyl- and methoxy-terminated oligo(ethylene glycol) alkane thiolates on gold. The surface adhesion propensity of human monocytes (cell line U937) was highly dependent on the lateral IgG density and indicated that there exists a distance between IgG-Fc on the surface where interactions with Fc gamma receptors are optimal. This well-defined platform allows for a careful control of the size and orientation of artificial IgG immune complexes, it is easily made compatible with, for example, cellular imaging, and it will become useful for in vitro studies on the importance of Fc gamma receptor interactions in chronic immune-mediated diseases.
authors.affiliation: Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden. dakan@ifm.liu.se
authors.forename: Daniel
authors.initials: D
authors.lastname: Kanmert
authors.forename: Helena
authors.initials: H
authors.lastname: Enocsson
authors.forename: Jonas
authors.initials: J
authors.lastname: Wetterö
authors.forename: Alf
authors.initials: A
authors.lastname: Kastbom
authors.forename: Thomas
authors.initials: T
authors.lastname: Skogh
authors.forename: Karin
authors.initials: K
authors.lastname: Enander
mesh_terms: D000473:Alkanes / Q000737:chemistry; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D058846:Antibodies, Monoclonal, Murine-Derived; D000936:Antigen-Antibody Complex / Q000276:immunology*; D018951:Antigens, CD20 / Q000737:chemistry; D019921:Biotinylation; D002448:Cell Adhesion; D002460:Cell Line; D004220:Disulfides / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D006046:Gold / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D018448:Models, Immunological; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D017452:Receptors, IgG / Q000378:metabolism; D000069283:Rituximab; D013499:Surface Properties
chemical_list: D000473:Alkanes; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D000936:Antigen-Antibody Complex; D018951:Antigens, CD20; D004220:Disulfides; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D017452:Receptors, IgG; D011092:Polyethylene Glycols; D000069283:Rituximab; D006046:Gold