title: The effect of the Rochagan on radiolabeling with (99m)Tc.
abstract: Radionuclides are used in nuclear medicine by variety of diagnostic procedures. The labeling of red blood cells (RBC) with (99m)Tc is a current method applied in clinical nuclear medicine. Drugs can alter this labeling and modify the disposition of the radiopharmaceuticals. The influence of Rochagan on the labeling of blood constituents with (99m)Tc was reported. Samples of blood were incubated with different concentrations of Rochagan (0%; 6.25%; 12.5%; 25%; 50%; 100%). Stannous chloride and (99m)Tc (3.7MBq/mL) were added. Plasma (P) and (RBC) were isolated and precipitated with thricloroacetic acid 5%. The insoluble (IF) and soluble fractions (SF) were separated. The %ATI in RBC, IF-P and IF-RBC were calculated. The %ATI on RBC decreased significantly (p<0.05) from control to all concentrations of Rochagan, respectively: 90.15 + or - 0.14(control) to 70.80 + or - 4.21; to 64.36 + or - 0.33; to 57.30 + or - 1.56; to 50.28 + or - 2.71; to 42.41 + or - 2.24; on IF-RBC, respectively: 84.70 + or - 0.87(control) to 67.16 + or - 4.38; to 63.63 + or - 2.92; to 59.02 + or - 3.17; to 43.75 + or - 1.00; to 24.15 + or - 0.94 and also on IF-P, respectively: 83.46 + or - 1.09(control) to 50.90 + or - 3.36; to 35.46 + or - 4.13; to 35.78 + or - 2.31; to 28.74 + or - 3.09; to 19.66 + or - 1.34. The analyses were performed by T-Student and Mann Whitney tests, p<0.05. This effect was probably due to products present in Rochagan that may complex with ions or have a direct/indirect effect on intracellular stannous ion concentration.
authors.affiliation: Universidade Federal do Rio Grande do Norte (UFRN), Centro de Biociências, Departamento de Microbiologia e Parasitologia. Av. Salgado Filho, 3000, Natal, RN 59078-970, Brasil. cechol@ufrnet.br
authors.forename: C M C X
authors.initials: CM
authors.lastname: Holanda
authors.forename: M F
authors.initials: MF
authors.lastname: Silva-Júnior
authors.forename: R C
authors.initials: RC
authors.lastname: Alves
authors.forename: V S A
authors.initials: VS
authors.lastname: Barbosa
authors.forename: R P
authors.initials: RP
authors.lastname: Silva
authors.forename: L G
authors.initials: LG
authors.lastname: Rocha
authors.forename: A C
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000000981:diagnostic imaging*; D006801:Humans; D009593:Nitroimidazoles / Q000737:chemistry / Q000378:metabolism*; D010949:Plasma / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium / Q000737:chemistry / Q000378:metabolism*; D017971:Tin Compounds / Q000737:chemistry / Q000378:metabolism; D014344:Trypanocidal Agents / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009593:Nitroimidazoles; D019275:Radiopharmaceuticals; D017971:Tin Compounds; D014344:Trypanocidal Agents; C023599:stannous chloride; D013667:Technetium; C009999:benzonidazole