title: Investigation of the sequence and length dependence for cell-penetrating prenylated peptides.
abstract: Cell penetrating peptides are useful delivery tools for introducing molecules of interest into cells. A new class of cell penetrating molecules has been recently reported-cell penetrating, prenylated peptides. In this study a series of such peptides was synthesized to examine the relationship between peptide sequence and level of peptide internalization and to probe their mechanism of internalization. This study revealed that prenylated peptides internalize via a non-endocytotic pathway regardless of sequence. Sequence length and identity was found to play a role in peptide uptake but prenylated sequences as short as two amino acids were found to exhibit significant cell penetrating properties.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Wollack
authors.forename: Nicholette A
authors.initials: NA
authors.lastname: Zeliadt
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Ochocki
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Mullen
authors.forename: George
authors.initials: G
authors.lastname: Barany
authors.forename: Elizabeth V
authors.initials: EV
authors.lastname: Wattenberg
authors.forename: Mark D
authors.initials: MD
authors.lastname: Distefano
mesh_terms: D000595:Amino Acid Sequence; D002463:Cell Membrane Permeability / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017368:Protein Prenylation
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides