title: Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission.
abstract: Challenge tests with Artemia four different development stages (nauplii, metanauplii, pseudoadults and adults) to white spot syndrome virus was carried out by immersion challenge and virus-phytoplankton adhesion route in order to asses the possibility of Artemia acting as a vector of WSSV to penaeid shrimp Litopenaeus vannamei postlarvae. The WSSV succeeded in infecting four stages Artemia, and nested-PCR detection for WSSV revealed positive results to virus-phytoplankton adhesion route. No mass mortalities were observed in penaeid shrimp postlarvae fed with WSSV-positive Artemia which exposed to WSSV by virus-phytoplankton adhesion route, whereas WSSV DNA detected in penaeid shrimp postlarvae by nested-PCR. By contrary, no WSSV-positive was detected in any animal fed with WSSV-negative Artemia. These results indicated that Artemia could serve as a vector in WSSV transmission.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education Fisheries College, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Jia-Song
authors.initials: JS
authors.lastname: Zhang
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
authors.forename: Yun-Wei
authors.initials: YW
authors.lastname: Dong
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Tian
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Cao
authors.forename: Zuo-Jia
authors.initials: ZJ
authors.lastname: Li
authors.forename: Dong-Chun
authors.initials: DC
authors.lastname: Yan
mesh_terms: D000818:Animals; D001156:Artemia / Q000821:virology*; D001179:Arthropod Vectors / Q000821:virology; D004279:DNA, Viral; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D008018:Life Cycle Stages; D033561:Penaeidae / Q000821:virology*; D010839:Phytoplankton / Q000821:virology*; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral