DNA-adducts in subjects exposed to urban air pollution by benzene and polycyclic aromatic hydrocarbons (PAHs) in Cotonou, Benin.
Air pollution effect on humans represents a major public health problem. Exposure to genotoxic compounds in the ambient air is evaluated using different biomarkers. In the present study we assessed DNA-adducts levels in apparently healthy people living and working in the city of Cotonou (Benin) in which exposure to air pollutants such as benzene and polycyclic aromatic hydrocarbons (PAHs) mainly benzo(a)pyrene has been evidenced. Rural inhabitants were enrolled as control group. Taxi-motorbike drivers, street food vendors, and gasoline salesmen were recruited in Cotonou whereas suburban residents were recruited in Godomey, 12 km from Cotonou. We found that taxi-motorbike drivers, roadside residents, street vendors, taxi-motor-bike drivers and gasoline sellers had significantly higher levels of DNA-adducts than suburban and village inhabitants (P < 0.001; post hoc, LSD). Means values were 24.6 ± 6.4, 23.78 ± 6.9, 34.7 ± 9.8, and 37.2 ± 8.1 in the exposed groups versus 2.1 ± 0.6 and 3.1 ± 0.8 adducts/10(8) nucleotides, in the two control groups, respectively. We did not find any significant difference within the high exposure groups and inside low exposure subgroups (namely suburban residents and villagers) because the mean individual exposure values to both PAHs and benzene were similar among subjects exposed in the city of Cotonou and those in suburban and village areas. However, there is significant interindividual variations in adducts levels that may reflect variation of genetic susceptibility factors. Ranges of adduct level/10(8) nucleotides were: 1-69, 1-76, 3-169, 4-124, 0-9, 0-8 adducts/10(8) for taxi-motorbike drivers, roadside residents, street vendors, gasoline sellers, suburban and village inhabitants, respectively. Our study demonstrated a clear-cut elevated level of DNA adducts in city residents than in none exposed people (or very low exposure levels people) and designate these city residents groups as people at risks for the chronic diseases possibly caused by benzene and PAHs.
Ayi-Fanou
Lucie
L
Laboratoire de Biochimie et de Biologie Moléculaire, FAST/UAC, Bénin.
Avogbe
Patrice H
PH
Fayomi
Benjamin
B
Keith
Gerard
G
Hountondji
Codjo
C
Creppy
Edmond E
EE
Autrup
Herman
H
Rihn
Bertrand Henri
BH
Sanni
Ambaliou
A
D000328:Adult; D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity* / Q000652:urine; D001345:Autoradiography; D001541:Benin; D001554:Benzene / Q000032:analysis / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D015415:Biomarkers / Q000652:urine; D018736:DNA Adducts / Q000378:metabolism*; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D008297:Male; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity* / Q000652:urine; D012424:Rural Population / Q000706:statistics & numerical data; D014505:Urban Population / Q000706:statistics & numerical data; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity; D055815:Young Adult
D000393:Air Pollutants; D015415:Biomarkers; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene; D001554:Benzene