[Relationship between primary ocular adnexal mucosa-associated lymphoid tissue lymphoma and eye infection].
OBJECTIVE
To study the role of pathogenic microorganisms commonly associated with chronic eye disease, including Chlamydia psittaci, Chlamydia trachomatis, Chlamydia pneumoniae, herpes simplex virus (HSV) type 1 and type 2, and adenovirus type 8 and type 19, in the development of primary ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma in Chinese patients.


METHODS
Sixty-eight archival cases of primary ocular adnexal lymphoproliferative lesions, including 38 cases of MALT lymphoma, 3 cases of non-MALT lymphoma and 27 cases of chronic inflammation, were enrolled into the study. DNA was extracted from the paraffin-embedded tissue samples. The presence of DNA of C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 were analyzed by multiplex touchdown enzyme time-release polymerase chain reaction (TETR-PCR).


RESULTS
All of the specimens yielded PCR products of over 100 base pairs and were thus suitable for TETR-PCR screening of infectious agents. The prevalence of DNA of C. psittaci, C. trachomatis and adenovirus type 19 were 0 in MALT lymphoma, non-MALT lymphoma and chronic inflammation. There were 2 cases positive for C. pneumoniae DNA, amongst the 38 cases of MALT lymphoma studied (5.3%, 2/38). HSV type 1, HSV type 2 and adenovirus type 8 DNA was found in each of the 3 patients with chronic inflammation.


CONCLUSIONS
The study indicates that C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 probably play little role in the pathogenesis of ocular adnexal MALT lymphoma in Chinese patients.
Zhang
Dan-dan
DD
Department of Pathology, Basic Medical College, Capital Medical University, Beijing 100069, China.
Liu
Hong-gang
HG
Li
Hai-yan
HY
Gao
Zi-fen
ZF
Zhou
Xiao-ge
XG
Jin
Ha-si
HS
Dong
Li-na
LN
Zhang
Jing
J
Gong
Li-ping
LP
D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D023521:Chlamydophila Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D015817:Eye Infections / Q000382:microbiology / Q000821:virology; D005134:Eye Neoplasms / Q000382:microbiology / Q000821:virology; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology / Q000821:virology; D009956:Psittacosis / Q000382:microbiology
D004269:DNA, Bacterial; D004279:DNA, Viral