[Studies on the primary structure of turnip yellow mosaic virus protein. II. Primary structure of the tryptic peptides of aminoethylated protein].
The sequence of the 7 tryptic peptides obtained from the oxidized protein of turnip yellow mosaic virus have been published in 1970. The other peptides obtained, being insoluble, could not be analysed. The protein was reduced, then alkylated with ethyleneimine and split with trypsin. In this paper we submit the sequence of the 14 peptides obtained, which have been separated on Dowex 50-X4 and Dowex 1-X2. These 14 peptides account for the 189 residues constituting the turnip yellow mosaic virus subunit (mol. wt 20,133).
Peter
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Laboratoire de Physiologie Végétale de l'Institut de Botanique de l'Université Louis Pasteur, 28, rue (I) Prot Goethe, 67083-Strasbourg Cédex, et Applications Biologiques, Centre Nucléaire, r, rue de Loess, 67200, Cronenbourg, France.
Stehelin
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Reinbolt
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Peter
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Duranton
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D000478:Alkylation; D000595:Amino Acid Sequence; D001388:Aziridines / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010100:Oxygen / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000737:chemistry*; D017806:Tymovirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
D001388:Aziridines; D010446:Peptide Fragments; D010455:Peptides; D014764:Viral Proteins; C033132:aziridine; D014357:Trypsin; D010100:Oxygen