Techniques for in vitro and in vivo fertilization in the rat.
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill. This chapter describes the protocols for in vitro and in vivo fertilization in the rat. Namely, sperm collection, sperm cryopreservation, pre-incubation of sperm, and insemination (co-culture with sperm and oocytes) for in vitro fertilization and intrauterine insemination for in vivo fertilization with fresh or frozen/thawed spermatozoa are provided.
Kashiwazaki
Naomi
N
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Seita
Yasunari
Y
Takizawa
Akiko
A
Maedomari
Naoki
N
Ito
Junya
J
Serikawa
Tadao
T
D000818:Animals; D018920:Coculture Techniques / Q000379:methods; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods* / Q000662:veterinary*; D007315:Insemination, Artificial / Q000379:methods* / Q000662:veterinary*; D008297:Male; D009865:Oocytes / Q000166:cytology; D051381:Rats / Q000502:physiology*; D017208:Rats, Wistar / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013094:Spermatozoa / Q000166:cytology