A cryptic role of a glycolytic-gluconeogenic enzyme (aldolase) in amino acid transporter turnover in Aspergillus nidulans.
In Aspergillus nidulans the fbaA1013 mutation results in reduced or total loss of growth on glycolytic and gluconeogenic carbon sources, respectively. It also negatively affects growth on several amino acids (including L-proline, L-glutamate or L-aspartate) that the fungus can use as nitrogen source on glycolytic carbon sources. Complementation of the fbaA1013 mutation using an A. nidulans genomic library resulted in cloning of the fbaA gene, which encodes a putative fructose 1,6-biphosphate aldolase (FBA), an enzyme involved in both glycolysis and gluconeogenesis. The fbaA1013 mutation is a chromosome rearrangement in the 5' regulatory region of the fbaA gene resulting in reduced or total loss of transcription in response to glycolytic and gluconeogenic carbon sources respectively. The fbaA gene is essential for growth. A functional FbaA protein is necessary for plasma membrane localization of the AgtA acidic amino acid (L-glutamate/L-aspartate) transporter, as the fbaA1013 mutation results in targeting to and presumably subsequent degradation of AgtA in the vacuole. Our results support a novel role of the FbaA protein that is, involvement in the regulation of amino acids transporters.
Roumelioti
Katerina
K
Institute of Biology, National Center for Scientific Research, Demokritos, Athens, Greece.
Vangelatos
Ioannis
I
Sophianopoulou
Vicky
V
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D016681:Genome, Fungal; D005943:Gluconeogenesis / Q000235:genetics; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D011392:Proline / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D004271:DNA, Fungal; D005656:Fungal Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; D002244:Carbon; D011392:Proline; D005634:Fructose-Bisphosphate Aldolase; D009584:Nitrogen