PEMF fails to enhance nerve regeneration after sciatic nerve crush lesion.
The use of electromagnetic fields has been reported to enhance peripheral nerve regeneration. This study aimed to identify the effects of a prolonged protocol of low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n = 17) and control (n = 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF group was exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for three weeks. Functional analysis was carried out weekly. After three weeks, the animals were euthanized, and histological, morphometric, oxidative stress, and TGF-beta1 analyses were performed. Functional analysis showed no differences between the groups. Histological appearance was similar between PEMF and control nerves. Morphometric assessment showed that the PEMF nerves trended toward decreased regeneration. The levels of free radicals were more pronounced in PEMF nerves, but were not associated with an increase in the content of the TGF-beta1/Smad signaling pathway. Prolonged PEMF regimen leads to delayed histological peripheral nerve regeneration and increased oxidative stress but no loss of function recovery.
Baptista
Abrahão Fontes
AF
Universidade Federal da Bahia, Biomorphology Department, Health Sciences Institute, Salvador, BA, Brazil.
Goes
Bruno Teixeira
BT
Menezes
Diego
D
Gomes
Flávia Carvalho Alcantara
FC
Zugaib
João
J
Stipursky
Joice
J
Gomes
Joyce R S
JR
Oliveira
Júlia Teixeira
JT
Vannier-Santos
Marcos André
MA
Martinez
Ana Maria Blanco
AM
D000818:Animals; D007150:Immunohistochemistry; D055909:Magnetic Field Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D009186:Myelin Sheath / Q000473:pathology / Q000502:physiology; D009409:Nerve Crush; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology*; D020426:Sciatic Neuropathy / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D015398:Signal Transduction; D051899:Smad2 Protein / Q000378:metabolism; D051900:Smad3 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D016896:Treatment Outcome
D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D053773:Transforming Growth Factor beta1