title: Application of bromoacetate-substituted beta-CD-bonded silica particles as chiral stationary phase for HPLC.
abstract: Bromoacetate-substituted [3-(2-O-beta-cyclodextrin)-2-hydroxypropoxy]propylsilyl-appended silica particles (BACD-HPS), an important and useful synthetic intermediate for preparation of novel types of macrocycles-capped beta-CD-bonded silica particles including crown ether/cyclam/calix[4]arene-capped beta-CD-bonded silica particles, have been prepared and used as chiral stationary phase for HPLC. This synthetic stationary phase is characterized by means of elemental analysis. For the first time, the chromatographic behavior of BACD-HPS was systematically evaluated with several disubstituted benzenes and some chiral drug compounds under both normal and RP conditions in HPLC. The results show that BACD-HPS has excellent selectivity for the separation of aromatic positional isomers and chiral isomers of some drug compounds when used as stationary phase in HPLC.
authors.affiliation: Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Sennappan K
authors.initials: SK
authors.lastname: Thamarai Chelvi
authors.forename: E L
authors.initials: EL
authors.lastname: Yong
authors.forename: Yinhan
authors.initials: Y
authors.lastname: Gong
mesh_terms: D000085:Acetates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000085:Acetates; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; C016274:bromoacetate; D012822:Silicon Dioxide