title: Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates.
abstract: This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.
authors.affiliation: Molecular Imaging Program at Stanford, Department of Radiology and Chemistry, Stanford University, Stanford, CA 94305-5484, USA.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Kim
authors.forename: Weston L
authors.initials: WL
authors.lastname: Daniel
authors.forename: Zuyong
authors.initials: Z
authors.lastname: Xia
authors.forename: Hexin
authors.initials: H
authors.lastname: Xie
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
authors.forename: Jianghong
authors.initials: J
authors.lastname: Rao
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000737:chemistry*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D009154:Mutation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D049408:Luminescent Agents; D011994:Recombinant Proteins; D006046:Gold; D008156:Luciferases; D020778:Matrix Metalloproteinase 2