Evaluation of anti-oxidant activities and total phenolic content of Chromolaena odorata.
The aim of this study was to assess the in vitro potential of chloroform extract of Chromolaena odorata leaves. The DPPH activity of the extract (0.1-5 mg/ml) was increased in a dose dependent manner, which was found in the range of 23.48-91.61% as compared to ascorbic acid (33.69-94.10%). The IC50 values of chloroform extract in DPPH radical, hydroxyl radical, nitric oxide, ABTS radical were obtained to be 0.31, 0.43, 0.28 and 1.32 mg/ml, respectively. However, the IC50 values for the standard ascorbic acid were noted to be 0.24, 0.41, 0.23 and 1 mg/ml, respectively. Measurement of total phenolic content of the chloroform extract of C. odorata was achieved using Folin-Ciocalteau reagent containing 242.2 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The results obtained in this study clearly indicate that C. odorata has a significant potential to use as a natural anti-oxidant agent.
Srinivasa Rao
K
K
Roland Institute of Pharmaceutical Sciences, Berhampur 760010, Orissa, India. ksrao108@gmail.com
Chaudhury
Pradeep Kumar
PK
Pradhan
Anshuman
A
D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D036604:Chromolaena / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl