Analysing the expression of protein kinase C eta in human hepatocellular carcinoma.
OBJECTIVE
Protein kinase Cs (PKCs) play important roles in cell proliferation, differentiation, apoptosis, migration and tumorigenesis. In this report, we investigated the expression of PKCeta in human hepatocellular carcinoma (HCC) tissues and explored its role in the development of HCC.


METHODS
We used real-time quantitative RT-PCR, mutation analysis, and immunohistochemical staining to analyse the expression of PKCeta in 50 pairs of human hepatocellular carcinoma (HCC) tissues.


RESULTS
Expression of PKCeta was down-regulated in 82% of HCC tissues and the reduction of PKCeta was associated with poorer long-term survival of HCC patients.


CONCLUSIONS
Reduced expression of PKCeta may represent a molecular lesion in the development of more aggressive disease of HCC.
Lu
Hsiu-Chin
HC
Departments of Laboratory Medicine and Clinical Research, Kaohsiung Medical University Hospital, Institute of Clinical Research, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chou
Fen-Pi
FP
Yeh
Kun-Tu
KT
Chang
Ya-Sian
YS
Hsu
Nicholas C
NC
Chang
Jan-Gowth
JG
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007527:Isoenzymes; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D055815:Young Adult
D004273:DNA, Neoplasm; D007527:Isoenzymes; C081975:protein kinase C eta; D011493:Protein Kinase C