title: NMR assignments of the DNA binding domain of Ml4 protein from Mesorhizobium loti.
abstract: Ml4 protein from Mesorhizobium loti has a 58% sequence identity with the Ros protein from Agrobacterium tumefaciens that contains a prokaryotic Cys(2)His(2) zinc finger domain. Interestingly, Ml4 is a zinc-lacking protein that does not contain the Cys(2)His(2) motif and is able to bind the Ros DNA target sequence with high affinity. Here we report the (1)H, (15)N and (13)C NMR assignments of the Ml4 protein DNA binding domain (residue 52-151), as an important step toward elucidating at a molecular level how this prokaryotic domain can overcome the metal requirement for proper folding and DNA-binding activity.
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli, via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Russo
authors.forename: Maddalena
authors.initials: M
authors.lastname: Palmieri
authors.forename: Ilaria
authors.initials: I
authors.lastname: Baglivo
authors.forename: Sabrina
authors.initials: S
authors.lastname: Esposito
authors.forename: Carla
authors.initials: C
authors.lastname: Isernia
authors.forename: Gaetano
authors.initials: G
authors.lastname: Malgieri
authors.forename: Paolo V
authors.initials: PV
authors.lastname: Pedone
authors.forename: Roberto
authors.initials: R
authors.lastname: Fattorusso
mesh_terms: D020561:Alphaproteobacteria / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002247:Carbon Isotopes / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D006859:Hydrogen / Q000737:chemistry; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004268:DNA-Binding Proteins; D009587:Nitrogen Isotopes; D011994:Recombinant Proteins; D006859:Hydrogen