A microfluidic system to evaluate intestinal absorption.
Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.
Imura
Yuki
Y
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Asano
Yasuyuki
Y
Sato
Kiichi
K
Yoshimura
Etsuro
E
D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D006801:Humans; D007408:Intestinal Absorption; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010539:Permeability; D013997:Time Factors