title: Optimal dose of busulfan for depleting testicular germ cells of recipient mice before spermatogonial transplantation.
abstract: Successful spermatogonial transplantation requires depletion of the host germ cells to allow efficient colonization of the donor spermatogonial stem cells. Although a sterilizing drug, busulfan (Myleran), is commonly used for preparing a recipient mouse before transplantation, the optimal dose of this drug has not yet been defined. The present study investigated the effects of different doses of busulfan (10-50 mg per kg body weight) on survival rate, testicular mass and histomorphology, and on the haploid spermatids and spermatozoa of male BALB/c mice. The results suggest that a dosage of 30 mg kg(-1) is optimal for the ablative treatment with busulfan used to prepare the recipient mice. This dose results in an adequate depletion of the host germ cells for colonization of donor-derived spermatogonial stem cells and causes the lowest death rate of the animals.
authors.affiliation: Department of Anatomy and Embryology, Wuhan University School of Medicine, China.
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Wang
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhou
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Yuan
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zheng
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage*; D002066:Busulfan / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D013093:Spermatogonia / Q000637:transplantation*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D002066:Busulfan