SCO3900, co-transcripted with three downstream genes, is involved in the differentiation of Streptomyces coelicolor.
SCO3900, a putative target gene of the developmental regulator WhiI of Streptomyces coelicolor, encodes a protein similar to PadR from Pediococcus pentosaceus. The SCO3900 disruption mutant exhibited a bald phenotype with sparse aerial hyphae. However, a single copy of SCO3900 under the control of its native promoter could not complement the mutant phenotype. A fragment containing SCO3900 and its downstream three genes completely restored the mutant phenotype to the wild-type. These suggested that the disruption of SCO3900 exerted a polar effect on its downstream genes. The co-transcription of SCO3899, SCO3898, and SCO3897 with SCO3900 was confirmed by the RT-PCR. Moreover, overexpression of SCO3900 in the wild-type strain caused the same phenotype as that of the disruption mutant. The results suggested that SCO3900 encodes a negative regulator participating in morphological differentiation of S. coelicolor by influencing the expression of its downstream genes.
Zhang
Guohua
G
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
Tian
Yuqing
Y
Hu
Kun
K
Feng
Chi
C
Tan
Huarong
H
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D009876:Operon; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000254:growth & development / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins