title: An immunochemical test for rapid screening of zearalenone and T-2 toxin.
abstract: An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 microg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.
authors.affiliation: Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
authors.forename: Evgenia Yu
authors.initials: EY
authors.lastname: Basova
authors.affiliation: Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia. Goryachevaiy@mail.ru.
authors.forename: Irina Yu
authors.initials: IY
authors.lastname: Goryacheva
authors.affiliation: Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
authors.forename: Tatiana Yu
authors.initials: TY
authors.lastname: Rusanova
authors.affiliation: Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Burmistrova
authors.affiliation: Chair of Hygiene and Technology of Milk, Ludwig-Maximilians-Universität München, Schonleutnerstrasse 8, 85764, Oberschleißheim, Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Dietrich
authors.affiliation: Chair of Hygiene and Technology of Milk, Ludwig-Maximilians-Universität München, Schonleutnerstrasse 8, 85764, Oberschleißheim, Germany.
authors.forename: Erwin
authors.initials: E
authors.lastname: Märtlbauer
authors.affiliation: Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
authors.forename: Christ'l
authors.initials: C
authors.lastname: Detavernier
authors.affiliation: Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
authors.forename: Carlos
authors.initials: C
authors.lastname: Van Peteghem
authors.affiliation: Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D055910:Antibodies, Immobilized; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques; D013058:Mass Spectrometry; D013605:T-2 Toxin / Q000032:analysis*; D014118:Toxins, Biological / Q000032:analysis*; D014908:Triticum / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D014118:Toxins, Biological; D015025:Zearalenone; D013605:T-2 Toxin