title: Resolving single-molecule assembled patterns with superresolution blink-microscopy.
abstract: In this paper we experimentally combine a recently developed AFM-based molecule-by-molecule assembly (single-molecule cut-and-paste, SMCP) with subdiffraction resolution fluorescence imaging. Using "Blink-Microscopy", which exploits the fluctuating emission of single molecules for the reconstruction of superresolution images, we resolved SMCP assembled structures with features below the diffraction limit. Artificial line patterns then served as calibration structures to characterize parameters, such as the labeling density, that can influence resolution of Blink-Microscopy besides the localization precision of a single molecule. Finally, we experimentally utilized the adjustability of blink parameters to demonstrate the general connection of photophysical parameters with spatial resolution and acquisition time in superresolution microscopy.
authors.affiliation: Applied Physics-Biophysics and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, D-80799 München, Germany.
authors.forename: Thorben
authors.initials: T
authors.lastname: Cordes
authors.forename: Mathias
authors.initials: M
authors.lastname: Strackharn
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Stahl
authors.forename: Wolfram
authors.initials: W
authors.lastname: Summerer
authors.forename: Christian
authors.initials: C
authors.lastname: Steinhauer
authors.forename: Carsten
authors.initials: C
authors.lastname: Forthmann
authors.forename: Elias M
authors.initials: EM
authors.lastname: Puchner
authors.forename: Jan
authors.initials: J
authors.lastname: Vogelsang
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld
mesh_terms: D000465:Algorithms; D002138:Calibration; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D015394:Molecular Structure; D053761:Nanocomposites / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D004247:DNA