High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells.
OBJECTIVE
To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells.


METHODS
A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis.


RESULTS
High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo.


CONCLUSIONS
High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.
Cui
Xiao-Nan
XN
Department of Pathology, Dalian Medical University, Dalian 116044, Liaoning Province, China.
Tang
Jian-Wu
JW
Song
Bo
B
Wang
Bo
B
Chen
Shan-Yan
SY
Hou
Li
L
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D045744:Cell Line, Tumor; D018093:Gelatinases / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000201:enzymology*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
D036341:Intercellular Signaling Peptides and Proteins; C512906:Ogn protein, mouse; D012333:RNA, Messenger; D018093:Gelatinases