title: A mouse model of anxiety molecularly characterized by altered protein networks in the brain proteome.
abstract: Recently, several attempts have been made to describe changes related to certain anxiety states in the proteome of experimental animal models. However, these studies are restricted by limitations regarding the number and correct identification of separated proteins. Moreover, the application of a systems biology approach to discover the molecular mechanisms of anxiety requires genetically homogenous inbred animal models. Therefore, we developed a novel mouse model of anxiety using a combination of crossbreeding (inbred for 35 generations) and behavioral selection. We found significant changes in 82 proteins in the total brain proteome compared to the control proteome. Thirty-four of these proteins had been previously identified in other anxiety, depression or repeated psychosocial stress studies. The identified proteins are associated with different cellular functions, including synaptic transmission, metabolism, proteolysis, protein biosynthesis and folding, cytoskeletal proteins, brain development and neurogenesis, oxidative stress, signal transduction. Our proteomics data suggest that alterations in serotonin receptor-associated proteins, in the carbohydrate metabolism, in the cellular redox system and in synaptic docking are all involved in anxiety.
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest, Pázmány P. stny. 1/c, Hungary.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Szego
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.forename: Attila
authors.initials: A
authors.lastname: Csorba
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Kompagne
authors.forename: Géza
authors.initials: G
authors.lastname: Müller
authors.forename: György
authors.initials: G
authors.lastname: Lévay
authors.forename: Attila
authors.initials: A
authors.lastname: Simor
authors.forename: Gábor
authors.initials: G
authors.lastname: Juhász
authors.forename: Katalin A
authors.initials: KA
authors.lastname: Kékesi
mesh_terms: D000818:Animals; D001007:Anxiety / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D005106:Exploratory Behavior; D006824:Hybridization, Genetic; D018782:Maze Learning; D051379:Mice; D008815:Mice, Inbred Strains; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome