Preparation of embryos for electron microscopy of the Drosophila embryonic heart tube.
The morphogenesis of the Drosophila embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell shape changes during embryonic development. One of the challenges faced in studying this structure is that the lumen of the heart tube, as well as the membrane features that are crucial to heart tube formation, are difficult to visualize in whole mount embryos, due to the small size of the heart tube and intra-lumenal space relative to the embryo. The use of transmission electron microscopy allows for higher magnification of these structures and gives the advantage of examining the embryos in cross section, which easily reveals the size and shape of the lumen. In this video, we detail the process for reliable fixation, embedding, and sectioning of late stage Drosophila embryos in order to visualize the heart tube lumen as well as important cellular structures including cell-cell junctions and the basement membrane.
Soplop
Nadine H
NH
Joint Graduate Program in Cell and Developmental Biology, UMDNJ-Graduate School of Biomedical Sciences and Rutgers, The State University of New Jersey, USA.
Patel
Rajesh
R
Kramer
Sunita G
SG
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000648:ultrastructure*; D006321:Heart / Q000196:embryology*; D008854:Microscopy, Electron / Q000379:methods*; D009206:Myocardium / Q000648:ultrastructure*