Effect of cefuroxime and moxifloxacin on Staphylococcus epidermidis adherence to intraocular lenses.
OBJECTIVE
To investigate and compare the effect of cefuroxime and moxifloxacin on adhesion of Staphylococcus epidermidis to intraocular lenses (IOLs).


METHODS
Experimental study.


METHODS
The 3-pieced hydrophobic acrylic lenses were contaminated with S. epidermidis (American Type Culture Collection 35983) solutions containing 108 colony-forming units. IOLs were inoculated into test tubes containing tryptic soy broth after being held in antibiotic solutions for 15 minutes. Sonication and vortex procedures were performed in order to remove all the remaining bacteria. From each tube 10 microL and 100 microL was taken and inoculated into sheep blood agar. The colonies were counted overnight. The statistical analyses were made using one-way ANOVA, Turkey Honestly Significant Differences test (HSD) and independent t tests, and a p value less than 0.05 was considered statistically significant.


RESULTS
Overall, the mean numbers of colony-forming units on the lenses that were held in control, cefuroxime, moxifloxacin 0.5 mg/0.1 mL and moxifloxacin 0.1 mg/0.1 mL solutions were 1398 (SE 10.01 x 10(3)), 29.9 (SE 1.16 x 10(3)), 0.23 (SD 0.04 x 10(3)), and 0.41 (SD 0.05 x 10(3)), respectively. The evaluation using one-way ANOVA and Turkey HSD tests revealed significant statistical differences among the groups (p = 0.000). The evaluation using independent t tests revealed significant statistical differences between the 2 moxifloxacin groups (p < 0.05).


CONCLUSIONS
Our results suggest that moxifloxacin and cefuroxime significantly inhibit bacterial adherence to IOLs. The effect of moxifloxacin on inhibition of bacterial adherence was significantly greater than that of cefuroxime. For this reason moxifloxacin might be considered as a better prophylactic agent.
Karadağ
Serhat
S
Ophthalmology Department, Kocaeli University, School of Medicine, Kocaeli, Turkey.
Ozkan
Berna
B
Karabaş
V Levent
VL
Alintaş
Ozgül
O
Yumuk
Zeki
Z
Cağlar
Yusuf
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001372:Aza Compounds / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002444:Cefuroxime / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024841:Fluoroquinolones; D007910:Lenses, Intraocular / Q000382:microbiology*; D008689:Methacrylates; D000077266:Moxifloxacin; D011108:Polymers; D011804:Quinolines / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000502:physiology*
D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D008689:Methacrylates; D011108:Polymers; D011804:Quinolines; C104020:poly(methylmethacrylate-co-ethylhexylmethacrylate); D002444:Cefuroxime; D000077266:Moxifloxacin