Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission.
Challenge tests with Artemia four different development stages (nauplii, metanauplii, pseudoadults and adults) to white spot syndrome virus was carried out by immersion challenge and virus-phytoplankton adhesion route in order to asses the possibility of Artemia acting as a vector of WSSV to penaeid shrimp Litopenaeus vannamei postlarvae. The WSSV succeeded in infecting four stages Artemia, and nested-PCR detection for WSSV revealed positive results to virus-phytoplankton adhesion route. No mass mortalities were observed in penaeid shrimp postlarvae fed with WSSV-positive Artemia which exposed to WSSV by virus-phytoplankton adhesion route, whereas WSSV DNA detected in penaeid shrimp postlarvae by nested-PCR. By contrary, no WSSV-positive was detected in any animal fed with WSSV-negative Artemia. These results indicated that Artemia could serve as a vector in WSSV transmission.
Zhang
Jia-Song
JS
The Key Laboratory of Mariculture, Ministry of Education Fisheries College, Ocean University of China, Qingdao, 266003, People's Republic of China.
Dong
Shuang-Lin
SL
Dong
Yun-Wei
YW
Tian
Xiang-Li
XL
Cao
Yi-Cheng
YC
Li
Zuo-Jia
ZJ
Yan
Dong-Chun
DC
D000818:Animals; D001156:Artemia / Q000821:virology*; D001179:Arthropod Vectors / Q000821:virology; D004279:DNA, Viral; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D008018:Life Cycle Stages; D033561:Penaeidae / Q000821:virology*; D010839:Phytoplankton / Q000821:virology*; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral