title: A combined cell membrane chromatography and online HPLC/MS method for screening compounds from Radix Caulophylli acting on the human alpha(1A)-adrenoceptor.
abstract: We have developed an online analytical method that combines alpha(1A)-adrenoceptor (alpha(1A)AR) cell membrane chromatography (alpha(1A)AR-CMC) with high performance liquid chromatography and mass spectrometry (HPLC/MS) for the identification of active components from Radix Caulophylli acting on the human alpha(1A)AR. Fractions retained by the alpha(1A)AR-CMC column were captured into a loop and the components were directly analyzed by combining an 8 port column switcher with an HPLC/MS system for separation and preliminary identification. Using methoxamine as a positive control drug, magnoflorine and caulophine from Radix Caulophylli were identified as the active molecules acting on the alpha(1A)AR. This new alpha(1A)AR-CMC-online-HPLC/MS method can be applied for screening active components acting on alpha(1A)AR from traditional Chinese medicines exemplified by Radix Caulophylli. This method will be of great utility in drug discovery using natural medicinal herbs as a source of novel compounds.
authors.affiliation: Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.forename: Meng
authors.initials: M
authors.lastname: Sun
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
mesh_terms: D018663:Adrenergic Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D001060:Aporphines / Q000494:pharmacology; D028145:Caulophyllum / Q000737:chemistry; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D013058:Mass Spectrometry; D008729:Methoxamine / Q000494:pharmacology; D009862:Online Systems; D018517:Plant Roots; D011485:Protein Binding; D018340:Receptors, Adrenergic, alpha-1 / Q000187:drug effects* / Q000378:metabolism; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods; D014162:Transfection
chemical_list: C547713:ADRA1A protein, human; D018663:Adrenergic Agents; D000316:Adrenergic alpha-Agonists; D001060:Aporphines; D004365:Drugs, Chinese Herbal; D018340:Receptors, Adrenergic, alpha-1; D008729:Methoxamine; C001670:magnoflorine