Influence of IL-1 gene polymorphism on the periodontal microbiota of HIV-infected Brazilian individuals.
This study investigated the association of IL-1A (+4845) and IL-1B (+3954) gene polymorphism with the subgingival microbiota and periodontal status of HIV-infected Brazilian individuals on highly active antiretroviral therapy (HAART). One hundred and five subjects were included in the study, distributed into 2 HIV groups [29 chronic periodontitis (CP+) and 30 periodontally healthy (H+)]; and 2 non-HIV groups (29 CP- and 17 H- patients). IL-1A and B were genotyped by PCR and restriction enzyme digestion. Thirty-three bacterial species were detected by checkerboard. Overall, we observed a prevalence of the allele 2 in the IL1-A and IL-1B polymorphism at 30.5% and 25.7%, respectively. Only 11.4% of all patients were composite genotype-positive, and 75% of those were HIV-infected. No significant associations between polymorphism of the IL-1 gene and periodontitis or HIV infection were observed. Likewise, no significant differences in the frequency and counts of any bacterial species were found between individuals with and without allele 2 (IL-1A or IL-1B). The data indicated that the IL-1 gene polymorphism is neither associated with periodontal destruction nor with high levels of subgingival species, including putative periodontal pathogens in HIV Brazilian individuals on HAART.
Gonçalves
Lucio de Souza
LS
Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil.
Ferreira
Sônia Maria Soares
SM
Souza
Celso Oliveira
CO
Colombo
Ana Paula Vieira
AP
D023241:Antiretroviral Therapy, Highly Active; D001419:Bacteria / Q000145:classification; D001938:Brazil; D055113:Chronic Periodontitis / Q000382:microbiology*; D004812:Epidemiologic Methods; D005838:Genotype; D005881:Gingiva / Q000382:microbiology*; D015658:HIV Infections / Q000188:drug therapy*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D007375:Interleukin-1; D053582:Interleukin-1alpha; D053583:Interleukin-1beta