Simultaneous determination of five-type hepatitis virus antigens in 5 min using an integrated automatic electrochemical immunosensor array.
An integrated automatic electrochemical immunosensor array has been designed for the simultaneous detection of 5-type hepatitis virus antigens (i.e. hepatitis A, hepatitis B, hepatitis C, hepatitis D, and hepatitis E). Initially, 5-type hepatitis virus antibodies were immobilized onto a self-made electrochemical sensor array using nanogold particles and protein A as matrices, and the immunosensor array was then used to capture their corresponding antigens from sample solution with a 1-step capture format. The detection is based on the potential change before and after the antigen-antibody reaction by using a 2-electrode system. The developed immunosensor array with allowed simultaneous determination of 5-type hepatitis virus antigens in 5 min. The detection limit of the sensor array was <or=1.0 ng/mL for most analytes. The intra- and inter-assay imprecision (CVs) of the sensor array were <or=7.3% and 8.1%, respectively. No nonspecific adsorption was evaluated, and the crossing talk between neighboring sites was <or=7.5% (CV). The developed immunoassay system offers promise for label-free, simple, rapid, cost-effective analysis of multi-analyte. Importantly, the chip-based immunosensor array could open new opportunities for high-throughput multi-analyte immunoassays in point-of-care testing of miniaturized lab-on-a-chip devices.
Tang
Dianping
D
Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education), and Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China. dianping.tang@hotmail.com
Tang
Juan
J
Su
Biling
B
Ren
Jingjing
J
Chen
Guonan
G
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018963:Hepatitis Antigens / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation*; D046228:Microarray Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012371:Robotics / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D018511:Systems Integration
D045424:Complex Mixtures; D018963:Hepatitis Antigens