Development of a blocking ELISA using a recombinant glycoprotein for the detection of antibodies to vesicular stomatitis New Jersey virus.
A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses.
Heo
Eun-Jeong
EJ
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 430-824, Republic of Korea.
Lee
Hyang-Sim
HS
Jeoung
Hye-Young
HY
Ko
Hyo-Rim
HR
Kweon
Chang-Hee
CH
Ko
Young-Joon
YJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D002460:Cell Line; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000235:genetics; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology; D006736:Horses; D011994:Recombinant Proteins / Q000235:genetics; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D018411:Spodoptera; D054260:Vesicular stomatitis New Jersey virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D011994:Recombinant Proteins