title: A proton NMR relaxation study of water dynamics in bovine serum albumin nanoparticles.
abstract: Water dynamics and compartmentation in glutaraldehyde cross-linked bovine serum albumin nanoparticles have been investigated by an integrated nuclear magnetic resonance (NMR) protocol based on water relaxation times and self-diffusion coefficients measurements. Multi-exponentially of water relaxation curves has been accounted for according to a diffusive and chemical exchange model (see B. P. Hills, S. F. Takacs and P. S. Belton, Mol. Phys., 1989, 67(4), 903, and Mol. Phys., 1989, 67(4), 913; E. Brosio, M. Belotti and R. Gianferri, in Food Science and Technology: New Research, ed. L. V. Greco and M. N. Bruno, Nova Science Publishers, Hauppauge (NY), 2008) that made it possible to single out water molecules in the molecular spaces in the interior of albumin nanoparticles, in the meso-cavities formed by packed nanoparticles and in the meniscus on top of the nanoparticles suspension. A quantitative rationalization of T(2) values of water different components allowed morphological information to be acquired as for the size of water filled compartments, while self-diffusion coefficient measurements of water excess or fluxed packed nanoparticles suspensions are describers of transport properties of soft biomaterials. The paper reports an NMR approach that can be seen as a general and relevant method to characterize excess-water-swollen soft biomaterials.
authors.affiliation: Department of Chemistry, University of Rome La Sapienza, P. le Aldo Moro, 5-00185 Roma, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Belotti
authors.forename: Andrea
authors.initials: A
authors.lastname: Martinelli
authors.forename: Raffaella
authors.initials: R
authors.lastname: Gianferri
authors.forename: Elvino
authors.initials: E
authors.lastname: Brosio
mesh_terms: D000818:Animals; D002417:Cattle; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry*; D011522:Protons; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D011522:Protons; D014867:Water; D012710:Serum Albumin, Bovine