Determination of ultra-trace aluminum in human albumin by cloud point extraction and graphite furnace atomic absorption spectrometry.
A cloud point extraction (CPE) method for the preconcentration of ultra-trace aluminum in human albumin prior to its determination by graphite furnace atomic absorption spectrometry (GFAAS) had been developed in this paper. The CPE method was based on the complex of Al(III) with 1-(2-pyridylazo)-2-naphthol (PAN) and Triton X-114 was used as non-ionic surfactant. The main factors affecting cloud point extraction efficiency, such as pH of solution, concentration and kind of complexing agent, concentration of non-ionic surfactant, equilibration temperature and time, were investigated in detail. An enrichment factor of 34.8 was obtained for the preconcentration of Al(III) with 10 mL solution. Under the optimal conditions, the detection limit of Al(III) was 0.06 ng mL(-1). The relative standard deviation (n=7) of sample was 3.6%, values of recovery of aluminum were changed from 92.3% to 94.7% for three samples. This method is simple, accurate, sensitive and can be applied to the determination of ultra-trace aluminum in human albumin.
Sun
Mei
M
Hefei National Laboratory for Physical Sciences on Microscale, University of Science and Technology of China, No. 96, Jinzhai Road, Hefei 230026, China. sunmei@ustc.edu.cn
Wu
Qianghua
Q
D000418:Albumins / Q000378:metabolism*; D000535:Aluminum / Q000032:analysis*; D011232:Chemical Precipitation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D011485:Protein Binding; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods* / Q000592:standards; D013501:Surface-Active Agents / Q000737:chemistry
D000418:Albumins; D013501:Surface-Active Agents; D000535:Aluminum