title: Free solution hydrodynamic separation of DNA fragments from 75 to 106,000 base pairs in a single run.
abstract: Gel electrophoresis is commonly used to separate DNA, but narrow capillaries or microchannels desired for high throughput efficient separations are difficult to fill with gels. We report here that a narrow capillary can be used to hydrodynamically separate a wide size range of DNA fragments in a single run without the need for gels, wall coatings, or an electric field. We also demonstrate that attractive separation is possible in a few minutes and that the separated DNA can be collected into individual fractions that remain viable for amplification via the polymerase chain reaction.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, USA.
authors.forename: Xiayan
authors.initials: X
authors.lastname: Wang
authors.forename: Vijaykumar
authors.initials: V
authors.lastname: Veerappan
authors.forename: Chang
authors.initials: C
authors.lastname: Cheng
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Randy D
authors.initials: RD
authors.lastname: Allen
authors.forename: Purnendu K
authors.initials: PK
authors.lastname: Dasgupta
authors.forename: Shaorong
authors.initials: S
authors.lastname: Liu
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D036103:Nanotechnology; D016133:Polymerase Chain Reaction; D012996:Solutions
chemical_list: D012996:Solutions; D004247:DNA