title: Using BCG, MPT-51 and Ag85 as antigens in an indirect ELISA for the diagnosis of bovine tuberculosis.
abstract: This study evaluated the Mycobacterium tuberculosis protein antigen MPT-51, the trimeric antigen 85 (Ag85) complex, and Bacillus Calmette-Guérin (BCG) in an indirect ELISA to diagnose bovine tuberculosis (TB) from serum samples. Serum was collected from 208 intra-dermal tuberculin test (ITT)-positive and 54 ITT-negative animals from a region where bovine TB is endemic. Using the Ag85 and BCG antigens, the indirect ELISA was able to discriminate ITT-positive from ITT-negative animals. This level of discrimination was not achieved when using the MPT-51 antigen. The highest sensitivity (Se) and specificity (Sp) of the test was found when BCG was used (Se, 82%; Sp, 91%). Further work in different epidemiological settings and with larger numbers of animals will be required to validate these findings.
authors.affiliation: Department of Microbiology, Immunology, Parasitology and Pathology, Research Center for Tropical Disease, Federal University of Goias, Rua Delenda Rezende de Melo, S/N, Setor Universitário, Goiânia, GO, Brazil.
authors.forename: Ediane Batista
authors.initials: EB
authors.lastname: da Silva
authors.forename: Bruna Daniella de Souza
authors.initials: BD
authors.lastname: Silva
authors.forename: Jerônimo Rafael Ruiz
authors.initials: JR
authors.lastname: Leon
authors.forename: André
authors.initials: A
authors.lastname: Kipnis
authors.forename: Isabel K F de Miranda
authors.initials: IK
authors.lastname: Santos
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Junqueira-Kipnis
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D009163:Mycobacterium bovis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium; C065036:antigen 85, Mycobacterium bovis; C072681:Mycobacterium tuberculosis antigens