title: TDP-43, a neuro-pathosignature factor, is essential for early mouse embryogenesis.
abstract: TDP-43 is a highly conserved and ubiquitously expressed nuclear protein. It has been implicated in the regulation of transcription, alternative splicing, translation, and neuronal plasticity. TDP-43 has also been shown to be a disease signature protein associated with several neurodegenerative diseases including amyotrophic lateral sclerosis. However, the correlation of the physiological functions of TDP-43 with these diseases remains unknown. We have used the gene targeting approach to disrupt the expression of TDP-43 in mouse. Loss of the TDP-43 expression results in peri-implantation lethality of mice between embryonic days (E) 3.5 and 6.5. Blastocysts of the homozygous Tardbp null mutants are morphologically normal, but exhibit defective outgrowth of the inner cell mass in vitro. Our data demonstrate the essential function of TDP-43 in peri-implantation stage during the embryo development, likely because of its involvement in multiple biological processes in a variety of cell types.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: Lien-Szu
authors.initials: LS
authors.lastname: Wu
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Cheng
authors.forename: Shin-Chen
authors.initials: SC
authors.lastname: Hou
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Yan
authors.forename: Si-Tse
authors.initials: ST
authors.lastname: Jiang
authors.forename: C-K James
authors.initials: CK
authors.lastname: Shen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential; D005838:Genotype; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins