Isolation and NMR characterization of fumonisin B2 and a new fumonisin B6 from Aspergillus niger.
A new fumonisin, fumonisin B(6) (1), has been isolated by cation-exchange and reverse-phase chromatography, together with fumonisin B(2) (2), from stationary cultures of the fungus Aspergillus niger NRRL 326. Analysis of mass spectrometric and NMR data determined that FB(6) is a positional isomer of FB(1) and iso-FB(1), having hydroxyl functions at C3, C4, and C5. Analysis of the NMR data for FB(2) showed very similar chemical shift values when compared to an authentic Fusarium FB(2) standard, strongly indicating identical molecules despite that an absolute stereochemical assignment of FB(2) from A. niger was not possible.
Månsson
Maria
M
Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Building 221, DK-2800 Kgs Lyngby, Denmark.
Klejnstrup
Marie Louise
ML
Phipps
Richard K
RK
Nielsen
Kristian F
KF
Frisvad
Jens C
JC
Gotfredsen
Charlotte H
CH
Larsen
Thomas O
TO
D001234:Aspergillus niger / Q000737:chemistry*; D037341:Fumonisins / Q000737:chemistry* / Q000302:isolation & purification*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D009183:Mycotoxins / Q000737:chemistry* / Q000302:isolation & purification*
D037341:Fumonisins; D009183:Mycotoxins; C548017:fumonisin B6; C056934:fumonisin B2