Design of a new electrogenerated polyquinone film substituted with glutathione. Towards direct electrochemical biosensors.
We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a "signal-on" direct electrochemical DNA sensor. The results were obtained with low target concentration (50nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target.
Reisberg
S
S
Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), Université Paris-Diderot, associé au CNRS, UMR 7086, 15 rue Jean-Antoine de Baïf, 75205 Paris, France.
Acevedo
D F
DF
Korovitch
A
A
Piro
B
B
Noel
V
V
Buchet
I
I
Tran
L D
LD
Barbero
C A
CA
Pham
M C
MC
D000588:Amines / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004553:Electric Conductivity; D004563:Electrochemistry; D005978:Glutathione / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry; D009285:Naphthoquinones / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000588:Amines; D015342:DNA Probes; D006878:Hydroxides; D009285:Naphthoquinones; D011108:Polymers; D004247:DNA; C031356:hydroxide ion; D005978:Glutathione; C005134:juglone