Validation data for HPLC/FLD determinations of ochratoxin A in red paprika and black pepper adopting a one-step clean-up procedure.
Validation data for the determination of ochratoxin A (OTA) in two spice matrices, red paprika and black pepper, were obtained for samples prepared with a simplified single-step clean-up column. Extracts of finely ground samples of red paprika and black pepper were prepared and applied to a Mycosep 229 Ochra clean-up column. The purified extract was then subjected to HPLC/FLD analysis. The relative standard deviation for repeatability (RSDr) of the method was 11.8% for red paprika and 9.9% for black pepper. The limit of detection (LOD) value (three times the noise) was estimated as corresponding to the response of an extract derived from a blank matrix (previously washed) and spiked at 1.0 microg kg(-1). The limit of quantitation (LOQ) (three times LOD) was 3.0 microg kg(-1). The performance of the one-step column clean-up procedure appears to be a suitable alternative to commonly used clean-up techniques and allows the precise determination of OTA in two complex matrices.
Bononi
M
M
Laboratori di Ricerche Analitiche e Tecnologiche su Alimenti e Ambiente, Di. Pro.Ve., Faculty of Agriculture, University of Milan, Milan 20133, Italy.
Gallone
F
F
Tateo
F
F
D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis*; D029222:Piper nigrum / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D009793:Ochratoxins; C025589:ochratoxin A