title: One-step purification of functional light-harvesting 2 complex from Rhodobacter sphaeroides.
abstract: Most common method for isolation of light-harvesting 2 complex (LH2) is very time-consuming. In our present work functional LH2 complex was one-step rapidly purified from Rhodobacter sphaeroides by metal-ion affinity chromatography. The purified LH2 complex exhibited high activity in light absorption and energy transfer. Our present work provides an efficient approach for preparation of functional LH2 complex.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, People's Republic of China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhao
authors.forename: Zongli
authors.initials: Z
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Tingzhang
authors.initials: T
authors.lastname: Hu
authors.forename: Yun
authors.initials: Y
authors.lastname: Tu
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D017830:Octoxynol / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D011994:Recombinant Proteins; D017830:Octoxynol