Ectopic bone formation from mandibular osteoblasts cultured in a novel human serum-derived albumin scaffold.
The aim of this study was to evaluate the ectopic bone formation using a novel serum-derived albumin scaffold and cultured human mandibular osteoblasts in nude mice. Osteoblasts were cultured with 10% human serum and plated in a novel spongy noncalcified protein scaffold prepared with plasmatic albumin crossed with a glutaraldehyde type agent. Hematoxylin-eosin staining revealed a bone-like extracellular matrix and in vitro mineralization was confirmed by von Kossa staining. Histological and immunohistochemical evaluation showed progression of mineralization in vivo. These results suggest the clinical feasibility of alveolar cells and albumin scaffold as a good alternative for bone regeneration.
Gallego
Lorena
L
Department of Oral and Maxillofacial Surgery, Cabueñes Hospital Gijón, Spain.
Junquera
Luis
L
Meana
Alvaro
A
Alvarez-Viejo
Mariá
M
Fresno
Manuel
M
D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D002828:Choristoma / Q000473:pathology; D006801:Humans; D008297:Male; D008334:Mandible / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D012709:Serum Albumin; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D012709:Serum Albumin