title: [Association of SNPs in N-formylpeptide receptor gene with susceptibility of aggressive periodontitis].
abstract: OBJECTIVE
  To explore the association of the single nucleotide polymorphisms (SNPs) in N-formylpeptide receptor (FPR) gene with the susceptibility of aggressive periodontitis (AgP).
  
  
  METHODS
  A total of 94 AgP patients and 73 healthy controls were entered into the study. Peripheral blood sample was obtained from each subject by venepuncture. Genomic DNA was isolated from each sample. The target fragment of FPR gene was amplified by PCR. The SNPs in FPR gene were detected by denature high performance liquid chromatography (DHPLC) combined with DNA sequencing.
  
  
  RESULTS
  There were two non-synonymous SNPs in the 370 bp FPR gene fragment: 289C/A and 301G/C. The 289C/A was a novel SNP. No variation in nucleotides 329 and 378 was detected. There were no statistically significant differences in distributions of the genotypes and alleles for FPR289 and FPR301 between AgP patients and healthy controls. Using multivariate logistic regression (adjusted for age and gender), it was showed that the adjusted ORs of AgP for the C(+) genotype and allele C of FPR301 combined with smoking were 5.74 and 5.20 respectively.
  
  
  CONCLUSIONS
  The presence of the C(+) genotype/allele C of FPR301 together with smoking conferred a higher risk for AgP. The result suggests that the SNPs in FPR gene may not be associated with the susceptibility of AgP in Chinese.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zhu
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Chen
authors.forename: Dong
authors.initials: D
authors.lastname: Shi
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Feng
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D044042:Receptors, Formyl Peptide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: D044042:Receptors, Formyl Peptide