Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe renal cell carcinoma from renal oncocytoma: a validation study combining metaphase and interphase analyses.
We investigated the usefulness of interphase fluorescence in situ hybridization (FISH) analysis to differentiate between 11 chromophobe renal carcinomas and 12 renal oncocytomas, showing different clinical outcomes, when compared with conventional metaphase cytogenetics by karyotyping. Karyotypically, 3 chromophobe renal cell carcinomas showed losses of chromosomes, 3 were polyploid, 1 was normal, and 4 failed to grow. Of 12 oncocytomas, 5 showed a normal numeric karyotype and 6 additional structural rearrangements. FISH on chromophobe renal cell carcinomas showed a high percentage of cases (10/11 [91%]) with multiple numeric losses among chromosomes 1, 2, 6, 10, and 17; this interphase pattern was observed irrespective of the 3 different metaphase karyotypes. Of 12 oncocytomas, 11 (92%) revealed a normal numeric chromosomal status showing at least 2 chromosomes without aneusomy by interphase FISH. The study demonstrates that indeed FISH performed on formalin-fixed, paraffin-embedded tissue can provide clinically useful information more reliably than karyotyping of most of these tumors.
Brunelli
Matteo
M
1Department of Pathology, University of Verona, Verona, Italy.
Delahunt
Brett
B
Gobbo
Stefano
S
Tardanico
Regina
R
Eccher
Albino
A
Bersani
Samantha
S
Cossu-Rocca
Paolo
P
Parolini
Claudia
C
Balzarini
Piera
P
Menestrina
Fabio
F
Cheng
Liang
L
Eble
John Nelson
JN
Martignoni
Guido
G
D018249:Adenoma, Oxyphilic / Q000175:diagnosis* / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D046888:Tissue Array Analysis
D004273:DNA, Neoplasm