A sensitive electrochemical approach for monitoring the effects of nano-Al2O3 on LDH activity by differential pulse voltammetry.
In this paper, a sensitive electrochemical approach for monitoring the effect of nano-Al(2)O(3) on lactate dehydrogenase (LDH) activity is established. It is based on the determination of reduction current of NAD(+) involved in enzyme promoting catalytic reaction of "pyruvate + NADH + H+ [see text]L D H lactate + NAD+" by differential pulse voltammetry (DPV). Various influencing factors including nano-type, nano-size, and adsorbed pollutant organics have been investigated. The experimental results show that the proposed electrochemical method is useful in monitoring and evaluating the toxic effects of nanoparticles, which might be suitable to the environmental pollutant's toxicity analysis.
Yao
Kaian
K
School of Chemistry and Chemical Engineering, State Key Laboratory of Pollution Control and Resource Reuse of China & MOE Key Laboratory for Life Science, Nanjing University, Nanjing, 210093, People's Republic of China. bisp@nju.edu.cn.
Huang
Deqian
D
Xu
Bolei
B
Wang
Na
N
Wang
Yingjie
Y
Bi
Shuping
S