Verification of biomarkers for diabetic retinopathy by multiple reaction monitoring.
Multiple reaction monitoring was used to verify target proteins in 3 groups of vitreous and plasma samples from 3 stages of diabetic retinopathy: macular hole (nondiabetic control), nonproliferative diabetic retinopathy, and proliferative diabetic retinopathy. Twelve target proteins were quantified using triple quadrupole LC-MS/MS and 3 methods to determine the transitions (information-dependent analysis, the MIDAS workflow, and the PeptideAtlas database). This study might be the first MRM experiment to analyze large numbers of clinical vitreous and plasma samples for biomarker verification. Consequently, several biomarker candidates were identified for use in further applications.
Kim
Kyunggon
K
Department of Biomedical Sciences, Cancer Research Institute, and Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
Kim
Sang Jin
SJ
Yu
Hyeong Gon
HG
Yu
Jiyoung
J
Park
Kyong Soo
KS
Jang
In-Jin
IJ
Kim
Youngsoo
Y
D015415:Biomarkers / Q000032:analysis*; D002853:Chromatography, Liquid; D003930:Diabetic Retinopathy / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D015415:Biomarkers