title: Iodide-induced chemokines and genes related to immunological function in cultured human thyroid follicles in the presence of thyrotropin.
abstract: BACKGROUND
  It is well known that iodide exacerbates thyroid function in subclinical hypothyroid patients with autoimmune thyroiditis. To investigate the immunological mechanism of iodine-induced thyroid dysfunction, we studied the effect of iodide in cultured human thyroid follicles, which respond to physiological concentrations of human thyrotropin (TSH) (0.3-10 microU/mL) and maintain the Wolff-Chaikoff effect.
  
  
  METHODS
  Thyroid follicles obtained from Graves' patients at subtotal thyroidectomy were precultured in medium containing 0.5% fetal calf serum and 10(-8) M iodide for 5 days, and then cultured with the medium containing bovine TSH (30 microU/mL) and low (10(-8)M) or high (10(-5)M) concentrations of iodide. After 3-72 hours of culture, the effect of iodide on thyroid cell mRNA expression was analyzed by microarray and reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  After 48 hours of culture, iodide nearly doubled the mRNA expression levels of the immunity-associated genes (intercellular adhesion molecule-1, transforming growth factor beta 1-induced protein, early growth response gene 1, guanylate-binding protein 1, and annexin A1) and decreased the mRNA expression of sodium-iodide symporter to less than 20%. Further, the mRNA expression levels of chemokines (CCL2, CXCL8, and CXCL14) increased nearly twofold, whereas their receptors did not show any significant response. Real-time polymerase chain reaction analyses confirmed that iodide increased the mRNA expression levels of these genes in a time- and concentration-dependent manner. Immunohistochemical studies revealed that the chemokines were expressed mainly in the thyroid follicular cells in addition to the immune cells. The iodide-induced increase in CCL2 was greater in thyroid follicles obtained from thyroid gland that had been moderately infiltrated with the immunocompetent cells.
  
  
  CONCLUSIONS
  We have demonstrated that iodide stimulates thyroid follicular cells to produce chemokines, particularly CCL2, CXCL8, and CXCL14. These chemokines and intercellular adhesion molecule-1 would attract immunocompetent cells into thyroid gland. These in vitro findings suggest that iodide at high concentrations may induce thyroid dysfunction through not only biochemical but also immunological mechanisms, particularly in patients with autoimmune thyroid disorders.
authors.affiliation: Thyroid Disease Institute, Kanaji Hospital, Tokyo, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tanigawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Emiko
authors.initials: E
authors.lastname: Yamada
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamada
authors.forename: Kazue
authors.initials: K
authors.lastname: Takano
authors.forename: Takao
authors.initials: T
authors.lastname: Obara
authors.forename: Kanji
authors.initials: K
authors.lastname: Sato
mesh_terms: D013956:Antithyroid Agents / Q000494:pharmacology*; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D006111:Graves Disease / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D055261:Immunogenetic Phenomena / Q000187:drug effects*; D007150:Immunohistochemistry; D007454:Iodides / Q000494:pharmacology*; D008713:Methimazole / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013972:Thyrotropin / Q000494:pharmacology*; D013997:Time Factors; D046509:Tissue Culture Techniques; D015854:Up-Regulation / Q000187:drug effects; D000099209:Sodium-Iodide Symporters
chemical_list: D013956:Antithyroid Agents; D018925:Chemokines; D007454:Iodides; D008713:Methimazole; D020033:Protein Isoforms; D012333:RNA, Messenger; D027981:Symporters; D013972:Thyrotropin; D000099209:Sodium-Iodide Symporters