Clickable, biocompatible, and fluorescent hybrid nanoparticles for intracellular delivery and optical imaging.
We report a general and facile approach for the fabrication of a new class of near monodisperse hybrid nanoparticles via RAFT polymerization and self-assembly in water. Furthermore, we combine a fluorescent inorganic silica core with a biocompatible polymer shell and a terminal unit susceptible to facile conjugations via click chemistry. A tailoring of the weight fractions of both components allows a tuning of the size of the formed aggregates. Fluorescent properties and the crosslinking into an organic-inorganic hybrid network are realized by copolymerizing a dye-functionalized monomer 1-pyrenebutyl acrylate and a trimethoxysilane-carrying one, (3-acryloxypropyl)trimethoxysilane. The potential of these stabilized and fluorescent nanoparticles as biocompatible carriers for intracellular delivery is demonstrated via in vitro experiments on lung cancer cells.
Müllner
Markus
M
Makromolekulare Chemie II and Bayreuther Zentrum fur Kolloide und Grenzflachen, Universitat Bayreuth, Germany.
Schallon
Anja
A
Walther
Andreas
A
Freitag
Ruth
R
Müller
Axel H E
AH
D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D055620:Optical Phenomena
D001672:Biocompatible Materials; D004337:Drug Carriers; D005456:Fluorescent Dyes