Critical evaluation of the volumetric "bottle effect" on microbial batch growth.
We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous reports.
Hammes
Frederik
F
Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dübendorf, Switzerland. frederik.hammes@eawag.ch
Vital
Marius
M
Egli
Thomas
T
D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D003470:Culture Media; D017753:Ecosystem; D004783:Environmental Microbiology; D005618:Fresh Water / Q000382:microbiology; D014734:Vibrio cholerae / Q000254:growth & development; D014881:Water Supply
D003470:Culture Media