A simple and rapid method of bacterial transformation.
Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar.
Wilharm
Gottfried
G
Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. wilharmg@rki.de
Lepka
Daniela
D
Faber
Franziska
F
Hofmann
Julia
J
Kerrinnes
Tobias
T
Skiebe
Evelyn
E
D040981:Acinetobacter baumannii / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D017633:Magnesium Silicates / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry; D014169:Transformation, Bacterial; D015008:Yersinia enterocolitica / Q000235:genetics
D017633:Magnesium Silicates; C001671:magnesium trisilicate