[Effects of thapsigargicin on Ca2+ movements in L1210 cells permeabilized with digitonin].
The effect of Thapsigargicin (TGC), a non-phorbol ester type tumor promoter, on Ca2+ movements has been investigated using L1210 mouse lymphoma cells. Ca2+ release from intact and digitonin permeabilized cells was evaluated using Fura-2 and Fura-3. TGC like Thapsigargin (TG) has the ability to discharge the intracellular Ca2+ stores and to increase intracellular free Ca2+ concentrations. TGC in a concentration dependent manner (0.16-16 nM) also inhibited cell growth and this effect was at least partially reversed by arachidonate.
Oztetik
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D000818:Animals; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D004072:Digitonin / Q000737:chemistry*; D007783:Lactones / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D012717:Sesquiterpenes / Q000494:pharmacology*
D007783:Lactones; D012717:Sesquiterpenes; C046319:thapsigargicin; D004072:Digitonin; D002118:Calcium