Effect of apolipoprotein a-I complex with tetrahydrocortisone on protein biosynthesis and glucose absorption by rat hepatocytes.
We studied the effect of apolipoprotein A-I-tetrahydrocortisone complex on (14)C glucose absorption and lactate accumulation and on the rate of protein biosynthesis in isolated rat hepatocytes. The presence of apolipoprotein A-I-tetrahydrocortisone complex in the incubation medium increased absorption of labeled glucose by hepatocytes by 52%, while lactate content in the conditioning medium increased 4-fold. The rate of protein biosynthesis increased by 80% in comparison with control cells. It is hypothesized that the increase in protein biosynthesis rate in hepatocytes under the effect of apolipoprotein A-I-tetrahydrocortisone complex is due to stimulation of energy metabolism, specifically, of its glycolytic component.
Sumenkova
D V
DV
Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Knyazev
R A
RA
Guschya
R S
RS
Polyakov
L M
LM
Panin
L E
LE
D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D013761:Tetrahydrocortisone / Q000737:chemistry / Q000494:pharmacology*
D016632:Apolipoprotein A-I; D019344:Lactic Acid; D013761:Tetrahydrocortisone; D005947:Glucose