Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.
Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3'-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.
Yamaji
Yasuyuki
Y
Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ayyamaji@mail.ecc.u-tokyo.ac.jp
Sakurai
Keitaro
K
Hamada
Koji
K
Komatsu
Ken
K
Ozeki
Johji
J
Yoshida
Akiko
A
Yoshii
Atsushi
A
Shimizu
Takumi
T
Namba
Shigetou
S
Hibi
Tadaaki
T
D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors; D054884:Host-Pathogen Interactions; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000235:genetics; D014176:Protein Biosynthesis; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*
D020648:Peptide Elongation Factor 1