Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores.
A straightforward synthesis of an anthrose-containing trisaccharide derived from Bacillus anthracis was achieved. Antibodies raised against this hapten provide a highly sensitive enzyme immunoassay with a detection limit of 8.5 pmol mL(-1). By investigating the specificity of the antibodies obtained using different mono-, di- and trisaccharide synthetic analogues, we demonstrated that the epitope was mainly made up of the methyl group at C-5, the butamido group at C-4 and the hydroxyl at C-3 of the anthrose unit, the other parts of the trisaccharide appearing little involved in the recognition.
Dhénin
Sandrine G Y
SG
Laboratoire des Glucides, UMR-CNRS 6219, Institut de Chimie de Picardie, Université de Picardie Jules Verne, 33 rue St Leu, F-80039, Amiens, France.
Moreau
Vincent
V
Nevers
Marie-Claire
MC
Créminon
Christophe
C
Djedaïni-Pilard
Florence
F
D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000276:immunology*; D000939:Epitopes; D007124:Immunoenzyme Techniques / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology; D014312:Trisaccharides / Q000032:analysis*
D000942:Antigens, Bacterial; D000939:Epitopes; D014312:Trisaccharides