Enhanced degradation of phenanthrene in a solid-liquid two-phase partitioning bioreactor via sonication.
The current article examined the feasibility of inducing improved delivery and degradation of phenanthrene in a solid-liquid partitioning bioreactor system at bench scale by means of ultrasonic energy input. Initial degradation rates of phenanthrene by a microbial consortium, delivered from Desmopan, were improved 2.7-fold in the presence of sonication relative to unsonicated controls. Results demonstrated that an operating window involving on/off sonication cycling improved substrate delivery and rational selection of ultrasound cycling profiles could lead to even further enhancements. Additionally, all results were obtained in a conventional bioreactor with commercial ultrasonic equipment and a commercially available polymer. Subsequent DGGE analysis demonstrated that the sonication cycles selected maintained consortium compositions, relative to control cases, and suggest that exposure would not reduce degradative capabilities under the periods of irradiation examined. Finally, consortium members were identified as belonging to the Pandoraea, Sphingobium, and Pseudoxanthomonas genera. Comparison of genetic sequences in the Ribosomal Database Project revealed that some of the bacterial members, identified at the strain level, had been previously observed in PAH degradations, while others have been reported only in the degradation of other aromatics, such as pesticides.
Isaza
Pedro A
PA
Department of Chemical Engineering, Queen's University, Kingston, Ontario, Canada.
Daugulis
Andrew J
AJ
D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D042521:Burkholderiaceae / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D010616:Phenanthrenes / Q000378:metabolism*; D013010:Sonication / Q000379:methods*; D042301:Sphingomonadaceae / Q000302:isolation & purification / Q000378:metabolism; D044166:Xanthomonadaceae / Q000302:isolation & purification / Q000378:metabolism
D010616:Phenanthrenes; C031181:phenanthrene