title: HLA-B*35-Px-mediated acceleration of HIV-1 infection by increased inhibitory immunoregulatory impulses.
abstract: A subset of HLA-B*35 alleles, B*35-Px, are strongly associated with accelerated HIV-1 disease progression for reasons that are not understood. Interestingly, the alternative set of B*35 subtypes, B*35-PY, have no detectable impact on HIV-1 disease outcomes, even though they can present identical HIV-1 epitopes as B*35-Px molecules. Thus, the differential impact of these alleles on HIV-1 disease progression may be unrelated to interactions with HIV-1-specific CD8(+) T cells. Here, we show that the B*35-Px molecule B*3503 binds with greater affinity to immunoglobulin-like transcript 4 (ILT4), an inhibitory MHC class I receptor expressed on dendritic cells, than does the B*35-PY molecule B*3501, even though these two B*35 molecules differ by only one amino acid and present identical HIV-1 epitopes. The preferential recognition of B*3503 by ILT4 was associated with significantly stronger dendritic cell dysfunction in in vitro functional assays. Moreover, HIV-1-infected carriers of B*3503 had poor dendritic cell functional properties in ex vivo assessments when compared with carriers of the B*3501 allele. Differential interactions between HLA class I allele subtypes and immunoregulatory MHC class I receptors on dendritic cells thus provide a novel perspective for the understanding of MHC class I associations with HIV-1 disease progression and for the manipulation of host immunity against HIV-1.
authors.affiliation: The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Boston, MA 02129. USA.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Huang
authors.forename: James J
authors.initials: JJ
authors.lastname: Goedert
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Sundberg
authors.forename: Thai Duong Hong
authors.initials: TD
authors.lastname: Cung
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Burke
authors.forename: Maureen P
authors.initials: MP
authors.lastname: Martin
authors.forename: Liliana
authors.initials: L
authors.lastname: Preiss
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Lifson
authors.forename: Mathias
authors.initials: M
authors.lastname: Lichterfeld
authors.forename: Mary
authors.initials: M
authors.lastname: Carrington
authors.forename: Xu G
authors.initials: XG
authors.lastname: Yu
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000209:etiology / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D018984:Epitopes, T-Lymphocyte; D005805:Genes, MHC Class I; D015497:HIV-1; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology; D011971:Receptors, Immunologic / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; D015235:HLA-B Antigens; C491341:LILRB2 protein, human; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic