Zoosporic plant pathogens produce bacterial autoinducer-2 that affects Vibrio harveyi quorum sensing.
The frequent coisolation of bacteria with Phytophthora and Pythium species suggests possible interspecies communication. Zoospore-free fluids (ZFF) from bacteria-free and nutrient-depleted zoospore suspensions were examined to investigate the production of autoinducer-2 (AI-2), a bacterial interspecies signal molecule, by zoosporic oomycetes. ZFF from Phytophthora nicotianae, Phytophthora sojae, and Pythium aphanidermatum triggered luminescence of the Vibrio harve7yi AI-2 reporter, indicating the presence of AI-2 in zoospore extracellular products and the potential of cross-kingdom communication between oomycetes and bacteria. The production of AI-2 by zoospores was confirmed by chemical assays. These results provide a new insight into the physiology and ecology of oomycetes.
Kong
Ping
P
Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Virginia Beach, VA 23455-3363, USA.
Lee
Bobby W K
BW
Zhou
Zhaohui Sunny
ZS
Hong
Chuanxue
C
D017930:Genes, Reporter; D006714:Homoserine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism*; D049449:Luminescence; D010838:Phytophthora / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000382:microbiology / Q000469:parasitology; D011775:Pythium / Q000302:isolation & purification / Q000378:metabolism*; D053038:Quorum Sensing / Q000187:drug effects*; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology
D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine