title: Design, synthesis, and amplification of DNA pools for in vitro selection.
abstract: Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
authors.forename: Bradley
authors.initials: B
authors.lastname: Hall
authors.forename: John M
authors.initials: JM
authors.lastname: Micheletti
authors.forename: Pooja
authors.initials: P
authors.lastname: Satya
authors.forename: Krystal
authors.initials: K
authors.lastname: Ogle
authors.forename: Jack
authors.initials: J
authors.lastname: Pollard
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis; D008967:Molecular Biology / Q000379:methods
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA