Generation of transgenic rats by microinjection of short DNA fragments.
Here we describe an efficient technique to generate transgenic rats by microinjection of short DNA fragments. We have focused on optimal conditions for superovulation of prepubescent females Sprague-Dawley (CD) strains to have good quality embryos, pseudopregnant females, zygotes preparation, optimal conditions for microinjection and embryo transfer into foster mothers.
Ménoret
Séverine
S
INSERM, UMR643, Nantes, France.
Remy
Séverine
S
Usal
Claire
C
Tesson
Laurent
L
Anegon
Ignacio
I
D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D008297:Male; D008845:Microinjections / Q000379:methods*; D010062:Ovulation Induction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic / Q000235:genetics*; D019076:Transgenes; D015053:Zygote / Q000166:cytology
D004247:DNA