Bis-4-aminobenzamidines: versatile, fluorogenic A/T-selective dsDNA binders.
N(1),N(3)-Bis(4-amidinophenyl)propane-1,3-diamine (BAPPA, R = H) is a bisaminobenzamidine fluorogenic derivative that displays a large increase in its emission fluorescence when bound to the minor groove of specific A/T DNA sites (K(d) for an AATT site approximately 79 +/- 6 nM). Moreover, the structural characteristics of BAPPA allow the easy introduction of functional groups that protrude out of the DNA surface.
Vázquez
Olalla
O
Departamento de Química Orgánica, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Sánchez
Mateo I
MI
Martínez-Costas
José
J
Vázquez
M Eugenio
ME
Mascareñas
José L
JL
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D003959:Diamines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D011407:Propane / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D003959:Diamines; C547492:N1,N3-bis(4-amidinophenyl)propane-1,3-diamine; D004247:DNA; D011407:Propane