Composition of the landfill microbial community as determined by application of domain- and group-specific 16S and 18S rRNA-targeted oligonucleotide probes.
The microbial community composition of colonized cotton and leachate samples from a landfill was quantified using small subunit (SSU) rRNA probes (quantitative rRNA hybridization). Relative quantification of bacteria, eukaryotes, and archaea revealed variations in the landfill microbial community between samples from different areas of the landfill site and indicated the presence of potentially novel archaea. Anaerobic fungi were quantified in rumen fluid samples but were not sufficiently abundant for direct detection in the landfill samples.
McDonald
James E
JE
School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool L697ZB, United Kingdom.
Allison
Heather E
HE
McCarthy
Alan J
AJ
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D017753:Ecosystem; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012037:Refuse Disposal; D012417:Rumen / Q000382:microbiology; D012756:Sheep; D012988:Soil Microbiology; D014874:Water Pollutants, Chemical
D015342:DNA Probes; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D014874:Water Pollutants, Chemical