L-Cysteine capped CdTe-CdS core-shell quantum dots: preparation, characterization and immuno-labeling of HeLa cells.
Functionalized CdTe-CdS core-shell quantum dots (QDs) were synthesized in aqueous solution via water-bathing combined hydrothermal method using L-cysteine (L-Cys) as a stabilizer. This method possesses both the advantages of water-bathing and hydrothermal methods for preparing high-quality QDs with markedly reduced synthesis time, and better stability than a lone hydrothermal method. The QDs were characterized by transmission electronic microscopy and powder X-ray diffraction and X-ray photoelectron spectroscopy. The CdTe-CdS QDs with core-shell structure showed both enhanced fluorescence and better photo stability than nude CdTe QDs. After conjugating with antibody rabbit anti-CEACAM8 (CD67), the as-prepared l-Cys capped CdTe-CdS QDs were successfully used as fluorescent probes for the direct immuno-labeling and imaging of HeLa cells. It was indicated that this kind of QD would have application potential in bio-labeling and cell imaging.
Zhang
Hongyan
H
Department of Chemistry, Northeastern University, Shenyang 110004, China.
Sun
Pan
P
Liu
Chang
C
Gao
Huanyu
H
Xu
Linru
L
Fang
Jin
J
Wang
Meng
M
Liu
Jinling
J
Xu
Shukun
S
D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots; D013440:Sulfides / Q000138:chemical synthesis / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D003545:Cysteine; D013691:Tellurium; C028337:cadmium telluride