title: Boron nutrition of cultured tobacco BY-2 cells. VI. Calcium is involved in early responses to boron deprivation.
abstract: Boron (B) deprivation induces various responses in plant cells, some of which can be observed very early. However, it has been unknown what kind of signal is generated by the stress. We found that B deprivation induced the expression of stress-responsive genes within 1 h in suspension-cultured tobacco BY-2 cells. The induction was largely suppressed by withholding medium Ca(2+) or by adding a Ca(2+) channel blocker. Analysis using aequorin-expressing cells showed that B-deprived cells took up more Ca(2+) than control cells. These results suggest that Ca(2+) influx plays a role in B deprivation stress signaling.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Koshiba
authors.forename: Masaru
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishihara
authors.forename: Toru
authors.initials: T
authors.lastname: Matoh
mesh_terms: D001895:Boron / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant; D013312:Stress, Physiological; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D001895:Boron; D002118:Calcium