Terbutryn toxicity to Danio rerio: effects of subchronic exposure on fish growth.
OBJECTIVE
The aim of this study was to investigate effects of subchronic exposure to sublethal levels of terbutryn on growth and on histopathological changes in Danio rerio.


METHODS
The acute toxicity tests were performed on the juvenile stage of Danio rerio according to OECD No. 203 Fish, Acute Toxicity Test. The juvenile growth tests were performed on D. rerio according to the OECD method No. 215. Fish at the age of 20 days were exposed to the terbutryn environmental concentration commonly detected in the Czech rivers (0.02 microg L-1) and the range of sublethal concentrations of terbutryn (0.06, 0.2, 0.6 and 2 mg L-1) for 28 days.


RESULTS
The 96hLC50 terbutryn mean value for the juvenile stage of D. rerio was 5.71 +/- 0.46 mg L-1. A significant decrease (p < 0.01) in the fish growth caused by terbutryn was observed in the concentration of 0.6 mg L-1. The value of NOEC was 0.2 mg L-1 and LOEC was 0.6 mg L-1 of terbutryn. We found the damage to tubular system of kidneys in the concentration of 0.6 mg L-1 of terbutryn.


CONCLUSIONS
The environmental concentration of terbutryn in the Czech rivers did not have any effects on growth and on histopathological changes in D. rerio and this concentration is lower than determined LOEC and NOEC values of terbutryn.
Plhalova
Lucie
L
Department of Veterinary Public Health and Toxicology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. lplhalova@vfu.cz
Macova
Stanislava
S
Haluzova
Ivana
I
Slaninova
Andrea
A
Dolezelova
Petra
P
Marsalek
Petr
P
Pistekova
Vladimira
V
Bedanova
Iveta
I
Voslarova
Eva
E
Svobodova
Zdenka
Z
D000818:Animals; D049628:Body Size / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects*; D018153:Czech Republic; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D045483:Rivers; D018675:Toxicity Tests; D014227:Triazines / Q000008:administration & dosage / Q000633:toxicity*; D014873:Water Pollutants / Q000008:administration & dosage / Q000633:toxicity; D015027:Zebrafish / Q000254:growth & development*
D006540:Herbicides; D014227:Triazines; D014873:Water Pollutants; C010347:terbutryne