Feature-level MALDI-MS characterization of in situ-synthesized peptide microarrays.
Characterizing the chemical composition of microarray features is a difficult yet important task in the production of in situ-synthesized microarrays. Here, we describe a method to determine the chemical composition of microarray features, directly on the feature. This method utilizes nondiffusional chemical cleavage from the surface along with techniques from MALDI-MS tissue imaging, thereby making the chemical characterization of high-density microarray features simple, accurate, and amenable to high-throughput.
Greving
Matthew P
MP
The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Kumar
Pallav
P
Zhao
Zhan-Gong
ZG
Woodbury
Neal W
NW
D000595:Amino Acid Sequence; D004563:Electrochemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides