title: Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.
abstract: A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal antibody was found to recognize a sequence of GREGEQEWGTPGSHVREETS. Further analysis with shorter pin-peptides with eight amino acid-long showed that the sequence of QEWGTPGS was an essential linear sequence of this epitope. When the QEW part of the sequence was replaced by alanine, the 92-2 monoclonal antibody did not bind to the substituted peptide, showing that those amino acids play an important role in the binding of the 92-2 monoclonal antibody.
authors.affiliation: Tamagawa University, Machida, Tokyo, 194-8610, Japan, shnmt920@agr.tamagawa.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Naganawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Tomita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takano-Ishikawa