Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus.
Glycine sarcosine methyltransferase (Gsm) and sarcosine dimethylglycine methyltransferase (Sdm) catalyze glycine betaine synthesis from glycine. Disruption of the M. xanthus gsmA (MXAN 7068) or sdmA (MXAN 3190) gene, encoding Gsm or Sdm homologue proteins, respectively, generated mutants that exhibited a longer lag period of growth and delayed spore germination under osmostress.
Kimura
Yoshio
Y
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, Japan 761-0795. kimura@ag.kagawa-u.ac.jp
Kawasaki
Shinji
S
Yoshimoto
Hinae
H
Takegawa
Kaoru
K
D000595:Amino Acid Sequence; D001622:Betaine / Q000378:metabolism*; D055786:Gene Knockout Techniques; D005998:Glycine / Q000378:metabolism*; D050938:Glycine N-Methyltransferase; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016941:Myxococcus xanthus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D009997:Osmotic Pressure; D016415:Sequence Alignment; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological; D014882:Water-Electrolyte Balance
D001622:Betaine; D050938:Glycine N-Methyltransferase; D005998:Glycine