title: [Effects of autologous mesenchymal stem cells transfected with heme oxygenase-1 gene transplantation on ischemic Swine hearts].
abstract: OBJECTIVE
  To observe the effect of intracoronary transfer of autologous HO-1 overexpressed MSCs in porcine model of myocardial ischemia (1 h)/reperfusion.
  
  
  METHODS
  Apoptosis was assayed and cytokine concentrations in supernatant were measured in cells exposed to hypoxia-reoxygen in vitro. In vivo, Chinese male mini-pigs were allocated to the following treatment groups: control group (saline), MSCs group (MSCs), MSCs transfected with pcDNA3.1-nHO-1 (HO-1-MSCs). 1 x 10(7) of autologous stem cells or identical volume of saline was injected intracoronary into porcine hearts 1 h after ischemia. MRI assay and postmortem analysis were assessed 3 months after stem cell transplantation.
  
  
  RESULTS
  In vitro, cell apoptosis rate post hypoxia-reoxygen was significantly reduced in HO-1-MSCs group (30.30% +/- 7.64%) compared with that in MSCs group (56.93% +/- 4.68%, P < 0.001) and LacZ-MSCs group (55.88% +/- 4.38%, P < 0.001), VEGF was also significantly upregulated in HO-1-MSCs group [(768.44 +/- 78.38) pg/ml] compared with that in MSCs group [(555.27 +/- 67.67) pg/ml, P < 0.001] and LacZ-MSCs group [(522.97 +/- 71.45) pg/ml, P < 0.001]. In vivo, cardiac function was significantly improved in both MSCs transplantation groups compared to saline group (all P < 0.05 vs.saline) and the left ventricular ejection fraction was significantly higher in HO-1-MSCs group compared with that in MSCs group at 3 months after transplantation (53.50% +/- 2.09% vs. 49.54% +/- 2.74%, P = 0.017), capillary density in the peri-infarct area was also significantly higher in HO-1-MSC group than that in MSCs group [(14.59 +/- 2.39)/HPF vs. (11.78 +/- 2.48)/HPF, P = 0.033].
  
  
  CONCLUSIONS
  Efficacy of HO-1 overexpressed MSCs on improving cardiac function and promoting angiogenesis was greater than those by MSCs in this porcine ischemia/reperfusion model.
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
authors.forename: Yi-bo
authors.initials: YB
authors.lastname: Jiang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Yao-liang
authors.initials: YL
authors.lastname: Tang
authors.forename: Gen-shan
authors.initials: GS
authors.lastname: Ma
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Qi
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yu-yu
authors.initials: YY
authors.lastname: Yao
authors.forename: Nai-feng
authors.initials: NF
authors.lastname: Liu
authors.forename: Cheng-xing
authors.initials: CX
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D005822:Genetic Vectors; D051547:Heme Oxygenase-1 / Q000235:genetics*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009203:Myocardial Infarction / Q000628:therapy*; D017202:Myocardial Ischemia / Q000628:therapy; D013552:Swine; D013556:Swine, Miniature; D014162:Transfection
chemical_list: D051547:Heme Oxygenase-1