Mechanism of the Synergistic Toxicity of Malononitrile and p -Nitrobenzaldehyde with Photobacterium phosphoreum.
In this study the mechanism of synergistic toxicity between malononitrile and p -nitrobenzaldehyde was revealed by examining their toxicodynamic and toxicokinetic interactions. The investigation of the toxicodynamic interactions focused on the interactions among malononitrile, p -nitrobenzaldehyde, and luciferase, and found that malononitrile does not induce luciferase to bind p -nitrobenzaldehyde. This result suggested that the synergistic toxicity does not arise from toxicodynamic interactions. Further research on toxicokinetic interactions compared the amounts of p -nitrobenzaldehyde being transported from the water phase to the biotic phase in two circumstances--in the presence of malononitrile and in its absence. The results of this research indicated that in the presence of malononitrile, more p -nitrobenzaldehyde is transported, and it is the increased amount of p -nitrobenzaldehyde that leads to the synergistic toxicity of malononitrile and p -nitrobenzaldehyde.
Lin
Zhifen
Z
Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, P. R. China, and National Key Research Station of Tropic Marine Biology, Sanya, P. R. China.
Zhang
Wenhuan
W
Wang
Liansheng
L
Yu
Hongxia
H
Wu
Chunde
C