Contributions to a better comprehension of redox-mediated decolouration and detoxification of azo dyes by a laccase produced by Streptomyces cyaneus CECT 3335.
The ability of a laccase (EC 1.10.3.2) produced by Streptomyces cyaneus CECT 3335 to decolourise and detoxify azo dyes was assessed. Results showed that a colour loss of 90% was achieved only in the presence of acetosyringone (0.1mM) acting as a redox mediator for the laccase. Toxicological analysis of the decolourised dyes revealed that there was no direct correlation between decolouration and detoxification; in fact, in the case of the dyes Methyl Orange and Orange II, a significant increase in toxicity was produced after the treatment. In contrast, a significant decrease in toxicity was observed after the decolouration of New Coccine and Chromotrope 2R. Finally, HPLC analysis of the dyes after treatment revealed the complete disappearance of both dyes and mediator and a concomitant appearance of new chromatographic peaks which could be responsible of the residual toxicity detected in some cases.
Moya
Raquel
R
Departamento de Microbiología y Parasitología, Universidad de Alcalá, Ctra. Madrid-Barcelona, Madrid, Spain.
Hernández
Manuel
M
García-Martín
Ana B
AB
Ball
Andrew S
AS
Arias
M Enriqueta
ME
D001391:Azo Compounds / Q000378:metabolism* / Q000633:toxicity; D001673:Biodegradation, Environmental / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004396:Coloring Agents / Q000378:metabolism* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008658:Inactivation, Metabolic; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D013053:Spectrophotometry; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology*; D013784:Textiles
D001391:Azo Compounds; D004396:Coloring Agents; D042845:Laccase