title: Evidence of mitochondrial dysfunction in obese adolescents.
abstract: OBJECTIVE
  Although obesity and weight gain generally are anticipated to be caused by an imbalance between energy intake and energy expenditure, the significance of thyroid hormones (TH) remains unclear. Examination of mitochondrial function may reflect intracellular thyroid hormone effect and elucidate whether a lower metabolic rate is present.
  
  
  METHODS
  In a group of 34 obese adolescents (age <16 years and body mass index above the age-related 95th percentile), and an age- and gender-matched group of 32 lean adolescent, thyroid stimulating hormone (TSH) and basal oxygen consumption were measured and mitochondrial function in peripheral blood monocytes was determined by flow cytometry.
  
  
  RESULTS
  Significant increase in TSH (3.06 +/- 1.56 mU/L vs. 2.33 +/- 0.91 mU/L, p < 0.05) and a decrease in VO2 (129 +/- 16 mL O2/m(2)*min vs. 146 +/- 15 mL O2/m(2)*min, p < 0.05) were observed in obese adolescents compared with lean adolescents. Flow cytometry analysis demonstrated a lower mitochondrial mass (6385 +/- 1962 a.u. vs. 7608 +/- 2328 a.u., p < 0.05) and mitochondrial membrane potential (11426 +/- 3861 a.u. vs. 14017 +/- 5536 a.u., p < 0.05) in obese adolescents compared with lean adolescents. These results are even more pronounced in adolescents with obese mothers.
  
  
  CONCLUSIONS
  In obese adolescents, the increased TSH and lowered VO2 propose a lowered basal metabolic rate and the impaired mitochondrial function suggests a decreased thyroid hormone stimulation of mitochondrial energy production. The maternal in-heritage is suggestive of a basal metabolic defect or mitochondrial resistance for TH.
authors.affiliation: The Mitochondrial Research Unit, Naestved Hospital, Naestved, Denmark. lkwl@regionsjaelland.dk
authors.forename: L
authors.initials: L
authors.lastname: Wilms
authors.forename: J
authors.initials: J
authors.lastname: Larsen
authors.forename: P L
authors.initials: PL
authors.lastname: Pedersen
authors.forename: J
authors.initials: J
authors.lastname: Kvetny
mesh_terms: D000293:Adolescent; D001481:Basal Metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000502:physiology*; D028361:Mitochondrial Diseases / Q000150:complications*; D009000:Monocytes / Q000378:metabolism; D009765:Obesity / Q000209:etiology* / Q000378:metabolism; D010101:Oxygen Consumption; D013963:Thyroid Hormones / Q000097:blood; D013972:Thyrotropin / Q000097:blood
chemical_list: D013963:Thyroid Hormones; D013972:Thyrotropin