title: Reconstruction of a mandibular defect with autogenous, autoclaved bone grafts and tissue engineering: An in vivo pilot study.
abstract: Reconstruction of bone defects with autogenous, autoclaved bone grafts has already been described but does have one major insuperable problem-the loss of the ostoinductive potential of the graft. In this study, we investigated if autogenous, autoclaved grafts in combination with tissue engineered bone can overcome this problem. An en-bloc resection was done in the mandible of eight pigs. The grafts were autoclaved and filled with autogenous, osseogen differentiated bone marrow cells and compared with four animals without bone marrow cells. After 120 days, the specimens were qualitatively and quantitatively evaluated by means of microradiography and light microscopy. Within the experimental group, osseous remodeling was detected in all cases and new bone formation was visible. Quantitative assessment of the osseous bridging of the osteotomy sites was significantly higher in the test group in comparison with the control group (p = 0.03). The histological evaluation by means of an osseous integration of the grafts revealed a statistically significant difference between both groups as well (p = 0.01). The results of this study indicate that the method investigated hereby represents a further possibility in the therapy of bony defects, such as those arising as a result of tumor operations.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuernberg, Glueckstrasse 11, 91054 Erlangen, Germany. cornelius.von-wilmowsky@uk-erlangen.de
authors.forename: Cornelius
authors.initials: C
authors.lastname: von Wilmowsky
authors.forename: Sophie
authors.initials: S
authors.lastname: Schwarz
authors.forename: Josef Matthias
authors.initials: JM
authors.lastname: Kerl
authors.forename: Safwan
authors.initials: S
authors.lastname: Srour
authors.forename: Michael
authors.initials: M
authors.lastname: Lell
authors.forename: Endre
authors.initials: E
authors.lastname: Felszeghy
authors.forename: Karl Andreas
authors.initials: KA
authors.lastname: Schlegel
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000379:methods; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry; D016025:Bone Transplantation / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D008334:Mandible / Q000473:pathology*; D010027:Osteotomy / Q000379:methods; D010865:Pilot Projects; D013242:Sterilization; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes