Evaluation of human neutrophil elastase inhibitory effect of iridoid glycosides from Hedyotis diffusa.
Five iridoid glycosides were isolated from the MeOH extract of Hedyotis diffusa, and their structures were elucidated as E-6-O-p-methoxycinnamoyl scandoside methyl ester (1), Z-6-O-p-methoxycinnamoyl scandoside methyl ester (2), E-6-O-p-feruloyl scandoside methyl ester (3), E-6-O-p-coumaroyl scandoside methyl ester (4), and Z-6-O-p-coumaroyl scandoside methyl ester (5) by interpretation of their spectroscopic data. All the isolated compounds were evaluated for human neutrophil elastase inhibitory effect, and compound 1 showed potent activity with an IC(50) value of 18.0muM. The molecular docking simulation suggested a structural model for the inhibition of human neutrophil elastase by compound 1.
Xu
Guang-Hua
GH
Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Kim
Young-Hee
YH
Chi
Seung-Wook
SW
Choo
Soo-Jin
SJ
Ryoo
In-Ja
IJ
Ahn
Jong-Seog
JS
Yoo
Ick-Dong
ID
D001665:Binding Sites; D003198:Computer Simulation; D032064:Hedyotis / Q000737:chemistry*; D006801:Humans; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
C548714:6-O-p-methoxycinnamoyl scandoside methyl ester; D039823:Iridoids; D010936:Plant Extracts; D011480:Protease Inhibitors; D019272:Leukocyte Elastase