title: A Langmuir monolayer study of the interaction of E1(145-162) hepatitis G virus peptide with phospholipid membranes.
abstract: E1(145-162), a peptide corresponding to the structural protein E1 of the GB virus C, has been shown earlier to bind at pH 7.4 to vesicles containing 1,2-dimyiristoyl-sn-glycero-3-phospho-rac-(1-glycerol)] (DMPG) and 1,2-dimyiristoyl-sn-glycero-3-phosphocholine (DMPC) phospholipids. To deepen the understanding of the interaction of E1(145-162) with the lipid membrane, in this paper, we report a detailed study of the surface properties of peptide, miscibility properties, and behavior of mixed monomolecular films of it and three phospholipids DMPG, DMPC, and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPG). These studies were performed using the Langmuir balance by means of surface adsorption studies, surface pressure-mean molecular area compression isotherms, and penetration kinetics. The Brewster angle microscopy (BAM) was used to study the morphological properties of pure peptide and the mixed monolayers. The results show us that the peptide showed surface activity concentration dependent when injected beneath a buffered solution (HEPES/NaCl, pH 7.4). This tendency to accumulate into the air/water interface confirms its potential capacity to interact with membranes; the higher penetration of peptide into phospholipids is attained when the monolayers are in the liquid expanded state and the lipids are charged negatively maybe due to its negative electric charge that interacts with the positive global charge of the peptide sequence. The area per molecule values obtained suggested that the main arrangement structure for E1(145-162) peptide is the alpha-helical at the air-water interface that agreed with computational prediction calculations. Miscibility studies indicated that mixtures become thermodynamically favored at low peptide molar fraction.
authors.affiliation: Physical Chemistry Department and Institute of Nanoscience and Nanotechnology, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain. mjesus_sanchez@hotmail.com
authors.forename: Maria Jesús
authors.initials: MJ
authors.lastname: Sánchez-Martín
authors.forename: Isabel
authors.initials: I
authors.lastname: Haro
authors.forename: M Asunción
authors.initials: MA
authors.lastname: Alsina
authors.forename: M Antonia
authors.initials: MA
authors.lastname: Busquets
authors.forename: Montserrat
authors.initials: M
authors.lastname: Pujol
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D054730:Protein Interaction Domains and Motifs; D013499:Surface Properties; D013816:Thermodynamics; D014759:Viral Envelope Proteins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D014759:Viral Envelope Proteins; C114224:envelope protein E1, GB virus C; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine