Influence of selective immunosuppressive drugs on the healing of exposed dogs' dental pulp capped with mineral trioxide aggregate.
BACKGROUND
Immunosuppressive drugs are used in clinical medicine for a variety of disorders, but their effects on the reparative capacity of the dental pulp are unknown. This study evaluated the influence of selected immunosuppressive drugs on pulpal tissue healing after direct pulp capping of mechanically exposed dog's teeth with mineral trioxide aggregate (MTA).


METHODS
Ten healthy male dogs were assigned into 5 experimental groups: a control group in which no drug was received and 4 experimental groups in which the immunosuppressive drugs prednisone, mycophenolate mofetil, sirolimus, and cyclosporine A were administered 45 days before the operative procedures and until the dogs were killed. Class V cavities were prepared on the buccal surfaces of 12 teeth in each dog. In each cavity, the pulp was exposed and capped with MTA. The pulpal tissue responses to capping material were assessed 65 days postoperatively.


RESULTS
Compared with the control group, variable responses was recorded in the groups treated with mycophenolate mofetil, sirolimus, and cyclosporine A, which were characterized by moderate to severe inflammatory reactions, tissue necrosis, and total absence of hard tissue bridging. Pulpal tissue responses in the group treated with prednisone were characterized by inflammatory cell infiltration, limited tissue necrosis, as well as partial to complete hard tissue bridging.


CONCLUSIONS
From these findings, it seemed evident that acceptable repair of the dentin-pulp complex, eg, wound healing with hard tissue formation after capping with MTA, is unlikely with mycophenolate mofetil, sirolimus, or cyclosporine A immunosuppressive drug therapy.
Mahmoud
Salah Hasab
SH
Conservative Dentistry Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. salahmahmoud2010@yahoo.com
Grawish
Mohammed El-Awady
Mel-A
Zaher
Ahmed Ragheb
AR
El-Embaby
Abeer
A
Karrouf
Gamal Ibrahim
GI
Sobh
Mohamed Abdel Kader
MA
D017607:Aluminum Compounds; D000818:Animals; D017610:Calcium Compounds; D016572:Cyclosporine / Q000009:adverse effects; D003782:Dental Pulp / Q000187:drug effects* / Q000276:immunology; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000276:immunology*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism; D004285:Dogs; D004338:Drug Combinations; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D009173:Mycophenolic Acid / Q000009:adverse effects / Q000031:analogs & derivatives; D009336:Necrosis / Q000139:chemically induced; D010087:Oxides; D011241:Prednisone / Q000494:pharmacology; D011671:Pulpitis / Q000139:chemically induced; D017640:Silicates; D020123:Sirolimus / Q000009:adverse effects; D014945:Wound Healing / Q000187:drug effects*
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D007166:Immunosuppressive Agents; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate; D016572:Cyclosporine; D009173:Mycophenolic Acid; D011241:Prednisone; D020123:Sirolimus