title: Molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16S rRNA gene PCR and gene sequencing techniques.
abstract: Polymerase chain reaction amplification of the universal 16S ribosomal RNA (rRNA) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. A total of 16 bacterial genera were identified including both Gram-positive and Gram-negative genera. Gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included Staphylococcus (most common genus isolated), Arthrobacter (2nd most common genus isolated), Brachybacterium, Exiguobacterium, Lactococcus, Microbacterium and Sporosarcina (next most common genera isolated) and finally, Bacillus, Brevibacterium, Frigoribacterium, Mycoplana and Pseudoclavibacter. Gram-negative organisms accounted for only 4/38 (10.5%) bacterial isolates and included the following genera, Brevundimonas, Lysobacter, Psychrobacter and Rhizobium. No gastrointestinal pathogens were detected. Although this study demonstrated a high diversity of the microorganisms present, only a few have been shown to be opportunistically pathogenic to humans and none of these organisms described have been described previously as having an inhalational route of infection and therefore we do not believe that the species of organisms identified pose a significant health and safety threat for immunocompetant individuals.
authors.affiliation: Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, UK.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Murayama
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kakinuma
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Maeda
authors.forename: Juluri R
authors.initials: JR
authors.lastname: Rao
authors.forename: Motoo
authors.initials: M
authors.lastname: Matsuda
authors.forename: Jiru
authors.initials: J
authors.lastname: Xu
authors.forename: Peter J A
authors.initials: PJ
authors.lastname: Moore
authors.forename: B Cherie
authors.initials: BC
authors.lastname: Millar
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Rooney
authors.forename: Colin E
authors.initials: CE
authors.lastname: Goldsmith
authors.forename: Anne
authors.initials: A
authors.lastname: Loughrey
authors.forename: M Ann S
authors.initials: MA
authors.lastname: McMahon
authors.forename: David A
authors.initials: DA
authors.lastname: McDowell
authors.forename: John E
authors.initials: JE
authors.lastname: Moore
mesh_terms: D000391:Air Microbiology; D000393:Air Pollutants / Q000302:isolation & purification*; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D004784:Environmental Monitoring; D008372:Manure / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D000393:Air Pollutants; D008372:Manure; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S