title: Bafilomycins produced in culture by Streptomyces spp. isolated from marine habitats are potent inhibitors of autophagy.
abstract: Five new bafilomycins, F (1) to J (5), have been isolated from laboratory cultures of two Streptomyces spp. obtained from marine sediments collected in British Columbia, and their structures have been elucidated by detailed analysis of spectroscopic data and the synthesis of model compounds. The new bafilomycins F (1), G (2), H (3), and J (5) along with several co-occurring known analogues showed potent inhibition of autophagy in microscopy and biochemical assays. The thiomorpholinone fragment present in bafilomycin F (1) has not previously been found in a natural product.
authors.affiliation: Department of Chemistry, University of British Columbia, Vancouver, B.C., Canada.
authors.forename: Gavin
authors.initials: G
authors.lastname: Carr
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.forename: Ana R
authors.initials: AR
authors.lastname: Díaz-Marrero
authors.forename: Brian O
authors.initials: BO
authors.lastname: Patrick
authors.forename: Helen
authors.initials: H
authors.lastname: Bottriell
authors.forename: Aruna D
authors.initials: AD
authors.lastname: Balgi
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Donohue
authors.forename: Michel
authors.initials: M
authors.lastname: Roberge
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Andersen
mesh_terms: D001343:Autophagy / Q000187:drug effects*; D001955:British Columbia; D018942:Macrolides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008386:Marine Biology; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D018942:Macrolides