title: Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the action of sodium hypochlorite (NaOCl) associated with an intracanal medication against Candida albicans and Enterococcus faecalis inoculated in root canals.
  
  
  METHODS
  Thirty-six human single-rooted teeth with single root canals were used. The canals were contaminated with C. albicans and E. faecalis for 21 days and were then instrumented with 1% NaOCl. The roots were divided into 3 groups (n=12) according to the intracanal medication applied: calcium hydroxide paste, 2% chlorhexidine (CHX) gel, and 2% CHX gel associated with calcium hydroxide. The following collections were made from the root canals: a) initial sample (IS): 21 days after contamination (control), b) S1: after instrumentation, c) S2: 14 days after intracanal medication placement; S3: 7 days after intracanal medication removal. The results were analyzed statistically by the Kruskal-Wallis test at 5% significance level.
  
  
  CONCLUSIONS
  Both 1% NaOCl irrigation and the intracanal medications were effective in eliminating E. faecalis and C. albicans inoculated in root canals.
authors.affiliation: Department of Restorative Dentistry, São Paulo State University, São José dos Campos, Brazil. marcia@fosjc.unesp.br
authors.forename: Marcia Carneiro
authors.initials: MC
authors.lastname: Valera
authors.forename: Katy Costa Godinho da
authors.initials: KC
authors.lastname: Silva
authors.forename: Lilian Eiko
authors.initials: LE
authors.lastname: Maekawa
authors.forename: Cláudio Antonio Talge
authors.initials: CA
authors.lastname: Carvalho
authors.forename: Cristiane Yumi
authors.initials: CY
authors.lastname: Koga-Ito
authors.forename: Carlos Henrique Ribeiro
authors.initials: CH
authors.lastname: Camargo
authors.forename: Raphael Silva e
authors.initials: RS
authors.lastname: Lima
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use*; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002176:Candida albicans / Q000187:drug effects*; D002710:Chlorhexidine / Q000627:therapeutic use; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide; D002710:Chlorhexidine