Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.
p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.
Yu
Lu
L
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 130062, Changchun, China.
Guo
Na
N
Yang
Yi
Y
Wu
Xiuping
X
Meng
Rizeng
R
Fan
Junwen
J
Ge
Fa
F
Wang
Xuelin
X
Liu
Jingbo
J
Deng
Xuming
X
D000935:Antifungal Agents / Q000633:toxicity*; D001547:Benzaldehydes / Q000633:toxicity*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D053858:Metabolic Networks and Pathways; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D013223:Statistics as Topic / Q000379:methods
D000935:Antifungal Agents; D001547:Benzaldehydes; C024896:4-anisaldehyde