Development of the custom polymeric materials specific for aflatoxin B1 and ochratoxin A for application with the ToxiQuant T1 sensor tool.
Two polymers were computationally designed with affinity to two of the most abundant mycotoxins aflatoxin B1 (AFB1) and ochratoxin A (OTA) for application in the ToxiQuant T1 System. The principle of quantification of AFB1 and OTA using the ToxiQuant T1 instrument comprised of a fluorimetric analysis of mycotoxins adsorbed on the polymer upon exposure to UV light. High affinity of the developed resins allowed the adsorption of both toxins as discrete bands on the top of the cartridge with detection limit as low as 1ng quantity of mycotoxins.
Piletska
Elena
E
Cranfield Health, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK. e.piletska@cranfield.ac.uk
Karim
Kal
K
Coker
Raymond
R
Piletsky
Sergey
S
D000327:Adsorption; D016604:Aflatoxin B1 / Q000032:analysis / Q000737:chemistry*; D002138:Calibration; D019295:Computational Biology; D016014:Linear Models; D008958:Models, Molecular; D009793:Ochratoxins / Q000032:analysis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009793:Ochratoxins; D011108:Polymers; C025589:ochratoxin A; D016604:Aflatoxin B1