Association of interleukin-18 gene polymorphisms with calcium oxalate kidney stone disease.
OBJECTIVE
The interleukin-18 (IL-18) encoding gene has three common single-nucleotide polymorphisms at -607C/A, -137G/C and +105A/C, which have been reported to be associated with several diseases. The aim of this study is to test whether IL-18 polymorphisms could act as genetic markers for renal stone disease.


METHODS
A control group of 104 healthy subjects, and 272 patients with recurrent calcium oxalate stones were examined. Polymerase chain reaction-based restriction endonuclease analysis was used to detect IL-18 polymorphisms.


RESULTS
The patient and control groups differed significantly in genotypic expression of the IL-18 +105A/C polymorphism. The prevalence of the A/C + C/C genotypes in the patients was higher than that in the controls. The allelic frequency of IL-18 +105A/C differed significantly between the patients and the controls. The odds ratio (OR) of the A/C heterozygote of IL-18 +105A/C associated with urolithiasis was 2.772. The OR of the A/C + C/C genotypes of IL-18 +105A/C associated with urolithiasis was 3.097. The OR per copy of the C allele of IL-18 +105A/C associated with urolithiasis was 4.143. There were also significant differences in the prevalence of genotype IL-18 -137G/C polymorphisms between the patients and controls. The distribution of the G/G homozygote in the patients was higher than that in the controls. There was no significant difference in genotype and allelic frequency at the IL-18 -607C/A polymorphism between patients and control subjects.


CONCLUSIONS
The results indicate that IL-18 +105A/C polymorphisms may play a role in the development of urolithiasis.
Lai
Kuang-Chi
KC
Department of Surgery, China Medical University Beigang Hospital, Yunlin, Taiwan.
Lin
Wei-Yong
WY
Man
Kee-Ming
KM
Tsai
Chou-Haung
CH
Chen
Huey-Yi
HY
Tsai
Fuu-Jen
FJ
Chen
Fun-Jou
FJ
Chen
Hsin-Yi
HY
Liu
Hsin-Ping
HP
Ho
Tsung-Jung
TJ
Huang
Po-Hsun
PH
Liu
Po-Len
PL
Lin
Feng-Yen
FY
Shen
Jui-Lung
JL
Liu
Jung-Tung
JT
Chen
Yung-Hsiang
YH
Chen
Wen-Chi
WC
D000328:Adult; D002129:Calcium Oxalate / Q000032:analysis; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D007669:Kidney Calculi / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
D020382:Interleukin-18; D002129:Calcium Oxalate