Over-expression of Nanog predicts tumor progression and poor prognosis in colorectal cancer.
We studied the expression and regulatory effects of ESC self-renewal molecule Nanog in colorectal cancer (CRC). Immunohistochemical analysis of 175 colorectal tumor samples showed that overexpression of Nanog was strongly correlated with poor prognosis, lymph node metastasis and Dukes classification for CRC. Univariate and multivariate survival analyses further indicated that Nanog expression was a potential prognostic factor for CRC. Gain-of-function analysis revealed that lentivirus-mediated Nanog overexpression promoted proliferation, motility and migration of human CRC cells. Interestingly, we found that Nanog played as both an inducer and a receipt of epithelial-mesenchymal transition (EMT) related signals. Nanog induced expression of Slug and Snail, two major regulator of EMT. Meanwhile, Nanog could also be regulated by Snail and initiated by TGF-β1. Our data demonstrate self-renewal gene Nanog has a prognostic role in CRC, which functions in progression of CRC by promoting proliferation, invasion, and motility of human CRC cells, and participates EMT process during CRC progression.
Meng
Hui-Min
HM
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510.
Zheng
Ping
P
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China Guangzhou 510515, People's Republic of China Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, People's Republic of China.
Wang
Xiao-Yan
XY
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 5105.
Liu
Chao
C
Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.
Sui
Hong-Mei
HM
Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.
Wu
Shao-Jie
SJ
Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.
Zhou
Jun
J
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.
Ding
Yan-Qing
YQ
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510.
Li
Jianming
J
Department of Pathology, Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China 51051.
D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D000071317:Nanog Homeobox Protein; D009361:Neoplasm Invasiveness; D011379:Prognosis
D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein