[Intranasal administration of low dosage recombinant human erythropoietin inhibits seizure in rats].
To investigate the effect of intranasal administration of low dosage recombinant human erythropoietin (r-HuEPO) on seizure in rats.



After intranasal or intraperitoneal administration of r-HuEPO, the behavioral and electroencephalographic changes were observed in pentylenetetrazol (PTZ) and maximal electroshock (MES) induced seizure or electrical amygdaloid-kindled seizure of rats.



Intranasal administration of low dosage r-HuEPO increased the seizure latency, and decreased the seizure grade and duration, and the number of convulsive episodes in PTZ induced seizure, with the most potential dosage of 2.4 IU. Intraperitoneal administration of r-HuEPO (3 000, 4 000 IU/kg) only decreased the seizure duration and number of convulsive episodes. The seizure grade, forelimb or hindlimb extension duration were decreased in MES-induced seizure by intranasal administration of 2.4 IU r-HuEPO. In addition, intranasal administration of 2.4 IU r-HuEPO decreased the seizure grade, generalized seizure duration and afterdischarge in electrical amygdaloid-kindled rats stimulated with generalized seizure threshold.



Intranasal administration of low dosage r-HuEPO can inhibit the seizure in rats.
Zhao
Hua-Wei
HW
College of Medicine, Zhejiang University, Hangzhou 310058, China.
Lu
Yuan
Y
Li
Xu-Yun
XY
Xu
Zheng-Hao
ZH
Zhang
Zhong-Miao
ZM
Hu
Wei-Wei
WW
Chen
Zhong
Z
D000281:Administration, Intranasal; D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage*; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy*; D004921:Erythropoietin / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D010433:Pentylenetetrazole; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins
D000927:Anticonvulsants; D011994:Recombinant Proteins; D004921:Erythropoietin; D010433:Pentylenetetrazole