title: Role of p53 mutation in the effect of boron neutron capture therapy on oral squamous cell carcinoma.
abstract: BACKGROUND
  Boron neutron capture therapy (BNCT) is a selective radiotherapy, being effective for the treatment of even advanced malignancies in head and neck regions as well as brain tumors and skin melanomas. To clarify the role of p53 gene, the effect of BNCT on oral squamous cell carcinoma (SCC) cells showing either wild- (SAS/neo) or mutant-type (SAS/mp53) p53 was examined.
  
  
  METHODS
  Cells were exposed to neutron beams in the presence of boronophenylalanine (BPA) at Kyoto University Research Reactor. Treated cells were monitored for modulations in colony formation, proliferation, cell cycle, and expression of cell cycle-associated proteins.
  
  
  RESULTS
  When SAS/neo and SAS/mp53 cells were subjected to BNCT, more suppressive effects on colony formation and cell viability were observed in SAS/neo compared with SAS/mp53 cells. Cell cycle arrest at the G1 checkpoint was observed in SAS/neo, but not in SAS/mp53. Apoptotic cells increased from 6 h after BNCT in SAS/neo and 48 h in SAS/mp53 cells. The expression of p21 was induced in SAS/neo only, but G2 arrest-associated proteins including Wee1, cdc2, and cyclin B1 were altered in both cell lines.
  
  
  CONCLUSIONS
  These results indicate that oral SCC cells with mutant-type are more resistant to BNCT than those with wild-type p53, and that the lack of G1 arrest and related apoptosis may contribute to the resistance. At a physical dose affecting the cell cycle, BNCT inhibits oral SCC cells in p53-dependent and -independent manners.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Osaka University Graduate School of Dentistry, Osaka, Japan. fujisan@dent.osaka-u.ac.jp
authors.forename: Yusei
authors.initials: Y
authors.lastname: Fujita
authors.forename: Itsuro
authors.initials: I
authors.lastname: Kato
authors.forename: Soichi
authors.initials: S
authors.lastname: Iwai
authors.forename: Koji
authors.initials: K
authors.lastname: Ono
authors.forename: Minoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sakurai
authors.forename: Ken
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yura
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D016754:Boron Neutron Capture Therapy; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D009062:Mouth Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D009154:Mutation; D011836:Radiation Tolerance / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53