title: Alpha1-fetoprotein: separation of two molecular variants by affinity chromatography with concanavalin A-agarose.
abstract: Alpha1-Fetoprotein present in fetal/newborn rat serum and in hepatoma-bearing human serum has been resolved into two molecular variants by concanavalin A-agarose affinity chromatography. The concanavalin A-reactive variant has been purified by gel filtration column chromatography, preparative block electrophoresis and immunoadsorption affinity chromatography.
authors.affiliation: Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05401, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Smith
authors.forename: P C
authors.initials: PC
authors.lastname: Kelleher
mesh_terms: D000327:Adsorption; D000818:Animals; D000831:Animals, Newborn; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003208:Concanavalin A / Q000737:chemistry*; D004586:Electrophoresis; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051381:Rats; D015203:Reproducibility of Results; D012685:Sepharose / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000509:alpha-Fetoproteins; D003208:Concanavalin A; D012685:Sepharose