Single-gene transgenic mouse strains for reprogramming adult somatic cells.
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
Carey
Bryce W
BW
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Markoulaki
Styliani
S
Beard
Caroline
C
Hanna
Jacob
J
Jaenisch
Rudolf
R
D000818:Animals; D054337:Cell Dedifferentiation / Q000187:drug effects; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects / Q000235:genetics*; D004318:Doxycycline / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016678:Genome; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016254:Mutagenesis, Insertional; D009928:Organ Specificity; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics*
C045073:Cre recombinase; D019426:Integrases; D004318:Doxycycline