Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum.
In this study, we employed directed evolution and site-directed mutagenesis to screen thermostable mutants of a family 11 xylanase from Neocallimastix patriciarum, and found that the thermostability and specific activity are both enhanced when mutations (G201C and C60A) take place in the interior hydrophobic region of the enzyme. Far-ultraviolet circular dichroism analysis showed that the melting temperatures (T(m)) of the G201C and C60A-G201C mutants are higher than that of the wild type by about 10 and 12 degrees C, respectively. At 72 degrees C, their specific activities are about 4 and 6 times as that of the wild type, respectively. Homology modeling and site-directed mutagenesis demonstrated that the enhanced thermostability of the G201C and C60A-G201C mutants may be mainly attributed to a potential stronger hydrophobic interaction between the two well-packed cysteines at sites 50 and 201, rather than the disulfide bond formation which was ruled out by thiol titration with dithionitrobenzoic acid (DTNB). And the strength of such interaction depends on the packing of the side-chain and hydrophobicity of residues at these two sites. This suggests that cysteine could stabilize a protein not only by forming a disulfide bond, but also by the strong hydrophobicity itself.
You
Chun
C
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.
Huang
Qiang
Q
Xue
Huping
H
Xu
Yang
Y
Lu
Hong
H
D019943:Amino Acid Substitution / Q000235:genetics; D002942:Circular Dichroism; D019020:Directed Molecular Evolution; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D020088:Neocallimastix / Q000201:enzymology*; D017434:Protein Structure, Tertiary
D005656:Fungal Proteins; D050505:Mutant Proteins; D043364:Endo-1,4-beta Xylanases