Transfer of a phage T4 gene into Enterobacteriaceae, determined at the single-cell level.
The transfer range of phage genes was investigated at the single-cell level by using an in situ DNA amplification technique. After absorption of phages, a phage T4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. The gene transfer decreased the mutation frequencies in nonhost recipients.
Kenzaka
Takehiko
T
Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-kita, Tondabayashi 584-8540, Japan.
Nasu
Masao
M
Tani
Katsuji
K
D017122:Bacteriophage T4 / Q000235:genetics*; D001483:Base Sequence; D016971:Citrobacter freundii / Q000235:genetics / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D021902:Enterobacter aerogenes / Q000235:genetics / Q000821:virology; D004755:Enterobacteriaceae / Q000235:genetics* / Q000821:virology*; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D018628:Gene Dosage; D018014:Gene Transfer Techniques; D005814:Genes, Viral; D017404:In Situ Hybridization, Fluorescence; D009154:Mutation; D011513:Proteus mirabilis / Q000235:genetics / Q000821:virology; D012477:Salmonella enteritidis / Q000235:genetics / Q000821:virology; D015008:Yersinia enterocolitica / Q000235:genetics / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral