title: Potential biomarkers of muscle injury after eccentric exercise.
abstract: Proteomics was utilized to identify novel potential plasma biomarkers of exercise-induced muscle injury. Muscle injury was induced in nine human volunteers by eccentric upper extremity exercise. Liquid chromatography-mass spectrometry identified 30 peptides derived from nine proteins which showed significant change in abundance post-exercise. Four of these proteins, haemoglobin alpha chain, haemoglobin beta chain, alpha1-antichymotrypsin (ACT) and plasma C-1 protease inhibitor (C1 Inh), met the criterion for inclusion based on changes in at least two distinct peptides. ACT and C1 Inh peptides peaked earlier post-exercise than creatine kinase, and thus appear to provide new information on muscle response to injury.
authors.affiliation: Harbor-UCLA Medical Center, Torrance, CA, USA.
authors.forename: Kathy E
authors.initials: KE
authors.lastname: Sietsema
authors.forename: Fanyu
authors.initials: F
authors.lastname: Meng
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Yates
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Hendrickson
authors.forename: Andy
authors.initials: A
authors.lastname: Liaw
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Song
authors.forename: Eric P
authors.initials: EP
authors.lastname: Brass
authors.forename: Roger G
authors.initials: RG
authors.lastname: Ulrich
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D015331:Cohort Studies; D050718:Complement C1 Inhibitor Protein / Q000096:biosynthesis; D003402:Creatine Kinase / Q000378:metabolism; D015444:Exercise; D005260:Female; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D009132:Muscles / Q000293:injuries*; D010455:Peptides / Q000097:blood / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D000514:alpha 1-Antichymotrypsin / Q000097:blood
chemical_list: D015415:Biomarkers; D050718:Complement C1 Inhibitor Protein; D006454:Hemoglobins; D010455:Peptides; D000514:alpha 1-Antichymotrypsin; C020622:hemoglobin B; D003402:Creatine Kinase