title: [Hepatitis C virus genotyping using 5 nuclease real-time PCR and probes with oligodeoxyinosine linkers].
abstract: Hepatitis C virus (HCV) infection is a major cause of severe liver disease including liver cirrhosis and hepatocellular carcinoma. Genotyping became fundamental in the clinical assessment of patients with hepatitis C because the genotype of the hepatitis C virus determines the chance of therapeutic response and duration of treatment. We developed a new real-time PCR assay for genotyping of HCV with increased specificity due to a novel approach to dual-labeled probe design using oligodeoxyinosine linkers. The assay allows genotypes 1, 2, 3 to be distinguished and genotypes 4-6 with high specificity to be blocked. The analytical sensitivity (150 IU/ml) can be implemented. Of the 285 clinical samples genotyped using the developed assay, 45% were genotype 1; 6%, genotype 2; 49%, genotype 3. No discordant results with 5-UTR sequencing and commercial genotyping assays were obtained.
authors.forename: V E
authors.initials: VE
authors.lastname: Vedernikov
authors.forename: M K
authors.initials: MK
authors.lastname: Ivanov
authors.forename: M A
authors.initials: MA
authors.lastname: Prasolova
authors.forename: E V
authors.initials: EV
authors.lastname: Kandrushin
authors.forename: G M
authors.initials: GM
authors.lastname: Dymshits
mesh_terms: D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000737:chemistry / Q000235:genetics*; D006526:Hepatitis C / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral