title: [DRB1 * 1454: the common allele of human leukocyte antigen-DRB1 * 14 in Chinese Han populations].
abstract: OBJECTIVE
  To investigate and compare the distribution of HLA-DRB1 * 14 alleles between the southern and northern Chinese Han populations.
  
  
  METHODS
  Human leukocyte antigen (HLA)-DRB1 alleles of 436 southern and 713 northern Chinese Han bone marrow volunteers were genotyped by polymerase chain reaction (PCR)-sequence-based-typing (SBT) method, among them the DRB1 * 1401/1439/1454 ambiguous allele pairs were identified using DRB1 * 14 high-resolution PCR-sequence specific primer (SSP) kits. Also, the clinic samples previously reported as DRB1 * 1401 were re-genotyped using the same PCR-SSP kits. The allelic distribution of DRB1 * 14 in southern and northern Chinese Han population were compared by chi-square test method.
  
  
  RESULTS
  Eighty-one ambiguous allele pairs of DRB1 * 1401/1439/1454 and 54 clinic samples previously reported as DRB1 * 1401 were all identified as DRB1 * 1454. Among the 436 Southern Han donors, six subtypes of DRB1 * 14 allele were observed including DRB1 * 1454 (69.57%), DRB1 * 1402 (1.45%), DRB1* 1403 (1.45%), DRB1 * 1404 (4.35%), DRB1 * 1405 (20.29%) and DRB1 * 1407 (2.90%). In the 713 northern Han donors, a total of seven subtypes were observed including DRB1 * 1454 (35.48%), DRB1 * 1403 (12.90%), DRB1 * 1404 (6.45%), DRB1 * 1405 (37.63%), DRB1 * 1407 (4.30%), DRB1 * 1411 (1.08%) and DRB1 * 1412 (2.15%).
  
  
  CONCLUSIONS
  DRB1 * 1454 and DRB1 * 1405 were the most common alleles of HLA-DRB1 * 14 in Chinese Han populations. The distribution of HLA-DRB1 * 14 differ significantly between the southern and northern Chinese Han population, while DRB1 * 1405 showed similar distribution pattern in the two populations but DRB1 * 1454 had higher frequency in southern than in northern Chinese Han population.
authors.affiliation: Immunogenetics and Histocompatibility Testing Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong, 518035 P.R. China. chenglh99@yahoo.com.cn
authors.forename: Liang-hong
authors.initials: LH
authors.lastname: Cheng
authors.forename: Shi-zheng
authors.initials: SZ
authors.lastname: Jin
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zou
authors.forename: Su-qin
authors.initials: SQ
authors.lastname: Gao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Da-ming
authors.initials: DM
authors.lastname: Wang
mesh_terms: D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans
chemical_list: D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains