Identification of Anoectochilus based on rDNA ITS sequences alignment and SELDI-TOF-MS.
The internal transcribed spacer (ITS) sequences alignment and proteomic difference of Anoectochilus interspecies have been studied by means of ITS molecular identification and surface enhanced laser desorption ionization time of flight mass spectrography. Results showed that variety certification on Anoectochilus by ITS sequences can not determine species, and there is proteomic difference among Anoectochilus interspecies. Moreover, proteomic finger printings of five Anoectochilus species have been established for identifying species, and genetic relationships of five species within Anoectochilus have been deduced according to proteomic differences among five species.
Gao
Chuan
C
Institute of Medicinal Plant Development, Beijing Union Medical College/Chinese Academy of Medicinal Sciences, China.
Zhang
Fusheng
F
Zhang
Jun
J
Guo
Shunxing
S
Shao
Hongbo
H
D018744:DNA, Plant; D004275:DNA, Ribosomal; D008970:Molecular Weight; D029595:Orchidaceae / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018744:DNA, Plant; D004275:DNA, Ribosomal; D010940:Plant Proteins; D020543:Proteome