title: Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.
abstract: Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
authors.affiliation: University of Alabama at Birmingham, School of Medicine, Department of Pathology, Room P230, 619 19th St., South, Birmingham, AL 35249-7331, USA. moser@uab.edu
authors.forename: S A
authors.initials: SA
authors.lastname: Moser
authors.forename: S C
authors.initials: SC
authors.lastname: Mitchell
authors.forename: J D
authors.initials: JD
authors.lastname: Ruby
authors.forename: S
authors.initials: S
authors.lastname: Momeni
authors.forename: R C
authors.initials: RC
authors.lastname: Osgood
authors.forename: J
authors.initials: J
authors.lastname: Whiddon
authors.forename: N K
authors.initials: NK
authors.lastname: Childers
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D013290:Streptococcal Infections / Q000382:microbiology / Q000635:transmission; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification