title: Near-infrared reflectance spectroscopy predicts protein, starch, and seed weight in intact seeds of common bean ( Phaseolus vulgaris L.).
abstract: The objective of this study was to explore the potential of near-infrared reflectance (NIR) spectroscopy to determine individual seed composition in common bean ( Phaseolus vulgaris L.). NIR spectra and analytical measurements of seed weight, protein, and starch were collected from 267 individual bean seeds representing 91 diverse genotypes. Partial least-squares (PLS) regression models were developed with 61 bean accessions randomly assigned to a calibration data set and 30 accessions assigned to an external validation set. Protein gave the most accurate PLS regression, with the external validation set having a standard error of prediction (SEP) = 1.6%. PLS regressions for seed weight and starch had sufficient accuracy for seed sorting applications, with SEP = 41.2 mg and 4.9%, respectively. Seed color had a clear effect on the NIR spectra, with black beans having a distinct spectral type. Seed coat color did not impact the accuracy of PLS predictions. This research demonstrates that NIR is a promising technique for simultaneous sorting of multiple seed traits in single bean seeds with no sample preparation.
authors.affiliation: Biology Department, Florida A&M University, Tallahassee, Florida 32307, USA.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Hacisalihoglu
authors.forename: Bismark
authors.initials: B
authors.lastname: Larbi
authors.forename: A Mark
authors.initials: AM
authors.lastname: Settles
mesh_terms: D027805:Phaseolus / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013213:Starch / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D013213:Starch