BSA activated CdTe quantum dot nanosensor for antimony ion detection.
A novel fluorescent nanosensor for Sb(3+) determination was reported based on thioglycolic acid (TGA)-capped CdTe quantum dot (QD) nanoparticles. It was the first antimony ion sensor using QD nanoparticles in a receptor-fluorophore system. The water-soluable TGA-capped CdTe QDs were prepared through a hydrothermal route, NaHTe was used as the Te precursor for CdTe QDs synthesis. Bovine serum albumin (BSA) conjugated to TGA-capped CdTe via an amide link interacting with carboxyl of the TGA-capped CdTe. When antimony ion enters the BSA, the lone pair electrons of the nitrogen and oxygen atom become involved in the coordination, switching off the QD emission and a dramatic quenching of the fluorescence intensity results, allowing the detection of low concentrations of antimony ions. Using the operating principle, the antimony ion sensor based on QD nanoparticles showed a very good linearity in the range 0.10-22.0 microg L(-1), with the detection limit lower than 2.94 x 10(-8) g L(-1) and the relative standard deviation (RSD) 2.54% (n = 6). In a study of interferences, the antimony-sensitive TGA-QD-BSA sensor showed good selectivity. Therefore, a simple, fast, sensitive, and highly selective assay for antimony has been built. The presented method has been applied successfully to the determination of antimony in real water samples (n = 6) with satisfactory results.
Ge
Shenguang
S
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. chm_gesg@ujn.edu.cn
Zhang
Congcong
C
Zhu
Yuanna
Y
Yu
Jinghua
J
Zhang
Shuangshuang
S
D000818:Animals; D000965:Antimony / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D002417:Cattle; D036103:Nanotechnology; D045663:Quantum Dots; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D019187:Cadmium Compounds; D013864:Thioglycolates; D014874:Water Pollutants, Chemical; D012710:Serum Albumin, Bovine; D000965:Antimony; D013691:Tellurium; C028337:cadmium telluride