[An in vitro hepatic steatosis cell model for study of non-alcoholic fatty liver disease].
To establish an in vitro cell model to investigate hepatic steatosis of non-alcoholic fatty liver disease.



HepG2 cells cultured in MEM containing 10 % fetal bovine serum were divided into control group and model group. At 7 0%-80 % confluency, HepG2 cells in model group were exposed to a long-chain mixture of free fatty acids (oleate and palmitate) for 24 h, cells in control group were subject to fresh medium. Lipid droplets were observed with oil red O stain and electron microscope, triglyceride and malonaldehyde were detected by respective assay kits.



A large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However,malonaldehyde did not increase significantly compared with control group.



A large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However, malonaldehyde did not increase significantly compared with control group.
Liu
Jiang
J
Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003,China. liujiangxm@yahoo.com.cn
Li
You-Ming
YM
Chen
Shao-Hua
SH
Li
Wei-Ping
WP
D003470:Culture Media / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005234:Fatty Liver; D056945:Hep G2 Cells; D006801:Humans; D008954:Models, Biological; D065626:Non-alcoholic Fatty Liver Disease; D019301:Oleic Acid / Q000494:pharmacology; D010168:Palmitates / Q000494:pharmacology
D003470:Culture Media; D005230:Fatty Acids, Nonesterified; D010168:Palmitates; D019301:Oleic Acid