Expression of WISP3 and RhoC genes at mRNA and protein levels in inflammatory and noninflammatory breast cancer in Tunisian patients.
Previous studies have shown the expression WISP3 and RhoC in cell lines of inflammatory breast cancer (IBC). The aim in the current study was to compare the expression of both genes, in biopsy samples collected from Tunisian patients with localized or metastatic breast cancer and patients with IBC. We investigated 127 patients enrolled in Salah Azaiez Institute in Tunis. Using the RT-PCR, we showed the phenotype (WISP3-, RhoC+) is significantly associated with IBC tumors, while the (WISP3+, RhoC-)phenotype is mostly associated to non-IBC tumors. The frequencies of these tumor phenotypes are significantly different between these tumor groups (p = 10(- 7); relative risk or RR = 3.25; confidential interval or CI 95% = 1.90-5.53). Immunohistochemical test revealing the presence of WISP3 and RhoC proteins correlates with the expression in the biopsy of their encoding genes as detected by RT-PCR. In conclusion, it appears that WISP3 and RhoC genes expression status defines a molecular signature of IBC.
Marrakchi
Raja
R
Laboratoire de Génétique, Immunologie et Pathologies Humaines, Faculté des Sciences de Tunis, Campus Universitaire, Tunisia. raja.triki@laposte.net
Khadimallah
Ines
I
Ouerhani
Slah
S
Gamoudi
Amor
A
Khomsi
Fathi
F
Bouzaine
Hatem
H
Benamor
Mohamed
M
Bougatef
Karim
K
Mnif
Samia
S
Zitoun
Rayhana
R
Benna
Farouk
F
Boussen
Hammouda
H
Rahal
Khaled
K
Elgaaied
Amel Benammar
AB
D000328:Adult; D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D055511:CCN Intercellular Signaling Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020741:rho GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D000075024:rhoC GTP-Binding Protein
D055511:CCN Intercellular Signaling Proteins; C400081:CCN6 protein, human; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein