title: Temperature gradient focusing in miniaturized free-flow electrophoresis devices.
abstract: Temperature gradient focusing is a method to separate and focus any charged analytes even without accessible isoelectric point, and has been already widely used in CE. In this paper, we demonstrate the application of temperature gradient focusing to free-flow electrophoresis. Besides focusing and separation experiments of proteins, the stability of the temperature gradient under flow conditions and the temperature dependence of fluorescence dyes have also been investigated.
authors.affiliation: ISAS - Institute for Analytical Sciences, Dortmund, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Becker
authors.forename: Abraham
authors.initials: A
authors.lastname: Mansouri
authors.forename: Cornelia
authors.initials: C
authors.lastname: Beilein
authors.forename: Dirk
authors.initials: D
authors.lastname: Janasek
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry; D008904:Miniaturization; D011506:Proteins / Q000302:isolation & purification; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins