title: Enhanced tolerance of transgenic tall fescue plants overexpressing 2-Cys peroxiredoxin against methyl viologen and heat stresses.
abstract: Plant 2-Cys peroxiredoxins (2-Cys Prx) has both peroxidase and chaperon function. We overexpressed an Arabidopsis 2-Cys Prx in transgenic tall fescue (Festuca arundinacea) plants to confer tolerance against heat and methyl viologen (MV) stress. Transgenic plants were generated by Agrobacterium-mediated genetic transformation, and integration and expression of the transgene was confirmed by Southern, northern and western blot analyses. Compared to control plants, transgenic plants had significantly less electrolyte leakage and thiobarbituric acid-reactive substances (TBARS) when exposed to heat or MV. Under heat stress (42 degrees C), transgenic plants maintained their chlorophyll fluorescence (Fv/Fm) for 24 h while control plants lost chlorophyll fluorescence very quickly. We conclude that the high levels of 2-Cys Prx proteins in transgenic plants protect leaves from oxidative damage probably due to chaperon activity.
authors.affiliation: Division of Applied Life Sciences, BK21 Program, IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Korea. [corrected]
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Iftekhar
authors.initials: I
authors.lastname: Alam
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Shamima Akhtar
authors.initials: SA
authors.lastname: Sharmin
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kwak
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000704:Analysis of Variance; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D031751:Festuca / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006358:Hot Temperature; D015227:Lipid Peroxidation; D010269:Paraquat / Q000494:pharmacology; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics; D010788:Photosynthesis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D019076:Transgenes
chemical_list: D029681:Arabidopsis Proteins; D017392:Thiobarbituric Acid Reactive Substances; D054464:Peroxiredoxins; D010269:Paraquat