title: Direct link between toluene degradation in contaminated-site microcosms and a Polaromonas strain.
abstract: Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene.
authors.affiliation: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
authors.forename: Shuguang
authors.initials: S
authors.lastname: Xie
authors.forename: Chunling
authors.initials: C
authors.lastname: Luo
authors.forename: Alison M
authors.initials: AM
authors.lastname: Cupples
mesh_terms: D001554:Benzene / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D002247:Carbon Isotopes; D042621:Comamonadaceae / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D012989:Soil Pollutants; D014992:Xylenes; D014050:Toluene; D001554:Benzene