title: GonadSAGE: a comprehensive SAGE database for transcript discovery on male embryonic gonad development.
abstract: Serial analysis of gene expression (SAGE) provides an alternative, with additional advantages, to microarray gene expression studies. GonadSAGE is the first publicly available web-based SAGE database on male gonad development that covers six male mouse embryonic gonad stages, including E10.5, E11.5, E12.5, E13.5, E15.5 and E17.5. The sequence coverage of each SAGE library is beyond 150K, 'which is the most extensive sequence-based male gonadal transcriptome to date'. An interactive web interface with customizable parameters is provided for analyzing male gonad transcriptome information. Furthermore, the data can be visualized and analyzed with the other genomic features in the UCSC genome browser. It represents an integrated platform that leads to a better understanding of male gonad development, and allows discovery of related novel targets and regulatory pathways.
authors.affiliation: Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. leetl@mail.nih.gov
authors.forename: Tin-Lap
authors.initials: TL
authors.lastname: Lee
authors.forename: Yunmin
authors.initials: Y
authors.lastname: Li
authors.forename: Hoi-Hung
authors.initials: HH
authors.lastname: Cheung
authors.forename: Janek
authors.initials: J
authors.lastname: Claus
authors.forename: Sumeeta
authors.initials: S
authors.lastname: Singh
authors.forename: Chandan
authors.initials: C
authors.lastname: Sastry
authors.forename: Owen M
authors.initials: OM
authors.lastname: Rennert
authors.forename: Yun-Fai Chris
authors.initials: YF
authors.lastname: Lau
authors.forename: Wai-Yee
authors.initials: WY
authors.lastname: Chan
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D047108:Embryonic Development / Q000235:genetics; D015870:Gene Expression; D023281:Genomics / Q000379:methods*; D006066:Gonads / Q000254:growth & development*; D008297:Male; D051379:Mice; D012984:Software