Description of Microvirga aerophila sp. nov. and Microvirga aerilata sp. nov., isolated from air, reclassification of Balneimonas flocculans Takeda et al. 2004 as Microvirga flocculans comb. nov. and emended description of the genus Microvirga.
Two bacterial strains, 5420S-12(T) and 5420S-16(T), isolated from air samples, were characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 5420S-12(T) was related phylogenetically to Microvirga subterranea FaiI4(T) (97.4 % sequence similarity) and Microvirga guangxiensis 25B(T) (97.1 %) and that strain 5420S-16(T) was closely related to Balneimonas flocculans TFB(T) (98.0 %) and Microvirga guangxiensis 25B(T) (97.2 %). The G+C content of the genomic DNA was 62.2 mol% for strain 5420S-12(T) and 61.5 mol% for strain 5420S-16(T). The major fatty acid was C(18 : 1)ω7c. The results of DNA-DNA hybridization and the phenotypic data showed that strains 5420S-12(T) and 5420S-16(T) could be distinguished from phylogenetically related species and represent two novel species within the genus Microvirga, for which the names Microvirga aerophila sp. nov. (type strain 5420S-12(T) =KACC 12743(T) =NBRC 106136(T)) and Microvirga aerilata sp. nov. (type strain 5420S-16(T) =KACC 12744(T) =NBRC 106137(T)) are proposed. Furthermore, the reclassification of Balneimonas flocculans as Microvirga flocculans comb. nov. (type strain TFB(T) =JCM 11936(T) =KCTC 12101(T) =IAM 15034(T) =ATCC BAA-817(T)) is proposed and an emended description of the genus Microvirga is provided.
Weon
Hang-Yeon
HY
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.|Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Son
Jung-A
JA
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Jo
Eun-Hye
EH
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Kim
Soo-Jin
SJ
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Kim
Yi-Seul
YS
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Ka
Jong-Ok
JO
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
D000391:Air Microbiology; D001482:Base Composition; D041841:Bradyrhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S