Site-directed delivery of ferritin-encapsulated gold nanoparticles.
Newly designed porter proteins, which catch gold nanoparticles and deliver the nanoparticles selectively to a silicon dioxide (SiO(2)) surface under the specific conditions were reported. Recombinant apoferritin subunits, each of which has gold-binding peptide and titanium-binding peptide at the C- and N-terminus, respectively, can efficiently encapsulate a gold nanoparticle. The bio-conjugate, a nanogold and surrounding mutant protein subunits, had a property which can deliver itself to the SiO(2) surface through the interaction. In theory, our genetically manipulated apoferritin subunits can encapsulate gold nanoparticles of various sizes, which is a promising property for applications involving surface plasmon resonance.
Zheng
B
B
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
Yamashita
I
I
Uenuma
M
M
Iwahori
K
K
Kobayashi
M
M
Uraoka
Y
Y
D000327:Adsorption; D016503:Drug Delivery Systems / Q000379:methods*; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D009154:Mutation; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D020349:Surface Plasmon Resonance
D010455:Peptides; D011994:Recombinant Proteins; D006046:Gold; D012822:Silicon Dioxide; D005293:Ferritins