title: The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
abstract: The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U1, U2, U5 and U4/U6 and pre-mRNAs. The mutations in the N-terminus, which prevent splicing to occur, include in vitro and in vivo identified phosphorylation sites of Prp4 kinase. These sites are highly conserved in the human ortholog U5-102 K. The results presented here demonstrate that structural integrity of the N-terminus is required to mediate a splicing event, but is not necessary for the assembly of spliceosomes.
authors.affiliation: Institute of Genetics, University of Braunschweig TU, Spielmannstr. 7, 38106 Braunschweig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Lützelberger
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Bottner
authors.forename: Wiebke
authors.initials: W
authors.lastname: Schwelnus
authors.forename: Susanne
authors.initials: S
authors.lastname: Zock-Emmenthal
authors.forename: Aleh
authors.initials: A
authors.lastname: Razanau
authors.forename: Norbert F
authors.initials: NF
authors.lastname: Käufer
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D000072260:RNA Splicing Factors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D017381:Spliceosomes / Q000378:metabolism*
chemical_list: C109024:PRP1 protein, S pombe; D000072260:RNA Splicing Factors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D029702:Schizosaccharomyces pombe Proteins; D017346:Protein Serine-Threonine Kinases; C489981:prp4 protein, S pombe