title: Generation of gene-specific mutated rats using zinc-finger nucleases.
abstract: The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat genes directly in the embryo. ZFNs can specifically disrupt target genes in cultured rat cells and in embryos from inbred and outbred strains, leading to permanently genetically modified animals. This technology allows for the rapid, targeted modification of the rat genome.
authors.affiliation: Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Aron M
authors.initials: AM
authors.lastname: Geurts
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Cost
authors.forename: Séverine
authors.initials: S
authors.lastname: Rémy
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Cui
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Jacob
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
authors.forename: Roland
authors.initials: R
authors.lastname: Buelow
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002478:Cells, Cultured; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D004624:Embryo Transfer; D005260:Female; D055786:Gene Knockout Techniques / Q000379:methods; D005796:Genes; D016678:Genome; D008297:Male; D015202:Protein Engineering / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics*; D014158:Transcription, Genetic; D016335:Zinc Fingers
chemical_list: D012333:RNA, Messenger; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific