A sensitive method for simultaneous determination of four macrolides by CE with electrochemiluminescence detection and its applications in human urine and tablets.
A sensitive method for simultaneous determination of azithromycin (AZI), acetylspiramycin (ACE), erythromycin (ERY), and josamycin (JOS) was developed by CE coupled with electrochemiluminescence detection with Ru(bpy)(3) (2+). The parameters related to separation and detection were investigated in detail. The four macrolides were well separated and detected within 6 min under the optimized conditions. The LOD (S/N=3) of AZI, ACE, ERY, and JOS were 1.2 x 10(-9), 7.1 x 10(-9), 3.9 x 10(-8) and 9.5 x 10(-8) mol/L, respectively. The LOQ (S/N=10) of AZI, ACE, ERY, and JOS in human urine were 8.2 x 10(-8), 2.5 x 10(-7), 8.9 x 10(-7) and 1.2 x 10(-6) mol/L, respectively. The recoveries of the four macrolides in human urine and pharmaceutical tablet samples were 85.0-104.0% at different concentration levels.
Liu
Yan-Ming
YM
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, PR China. liuym9518@sina.com
Shi
Yan-Mei
YM
Liu
Zhuan-Li
ZL
Tian
Wei
W
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D017963:Azithromycin / Q000032:analysis / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D018942:Macrolides / Q000032:analysis* / Q000652:urine; D009942:Organometallic Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry
D018942:Macrolides; D009942:Organometallic Compounds; D013607:Tablets; C103599:bis(bipyridyl)ruthenium(II); D015082:2,2'-Dipyridyl; D017963:Azithromycin