Glucose oxidase immobilized in alginate/layered double hydroxides hybrid membrane and its biosensing application.
A new type of amperometric glucose biosensor based on alginate (Alg)/layered double hydroxides (LDHs) organic-inorganic composite film is described. This hybrid material combines the advantages of an organic biopolymer, Alg and inorganic LDHs. Glucose oxidase (GOD) immobilized in the material maintained its activity. The composite films were characterized by UV-Vis. The results indicated that GOD retained the essential feature of its native structure in the composite film. The Alg/LDHs/GOD-modified platinum electrode exhibited a fast response to glucose (achieve 95% of the maximum in 10 s), which provided a linear response to glucose over a concentration range of 1.6 x 10(-5)-2 x 10(-3) M with a detection limit of 4 x 10(-5) M based on S/N = 3. Furthermore, the biosensor exhibited excellent long-term stability, and satisfactory reproducibility. It retained 87% of its original activity after being used for 28 days.
Ding
Shou-Nian
SN
School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, P. R. China. snding@seu.edu.cn
Shan
Dan
D
Xue
Huai-Guo
HG
Zhu
Dao-Bin
DB
Cosnier
Serge
S
D000464:Alginates / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry*; D008567:Membranes, Artificial; D010984:Platinum / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D006878:Hydroxides; D008567:Membranes, Artificial; D010984:Platinum; D020723:Glucuronic Acid; D005949:Glucose Oxidase; D005947:Glucose