title: Substrate adaptation of Trichophyton rubrum secreted endoproteases.
abstract: Trichophyton rubrum is the most common pathogen caused the dermatophytosis of nail and skin in human. The secreted proteases were considered to be the most important virulence factors. However, the substrates adaptation of T. rubrum secreted proteases is largely unknown. For the first time, we use the keratins from human nail and skin stratum corneum as the growth medium to investigate the different expression patterns of T. rubrum secreted endoproteases genes. During grow in both keratin-containing media SUB7 and MEP2 were the highest expressed gene in each family. These results indicated that SUB7 and MEP2 may be the dominant endoproteases secreted by T. rubrum during host infection and the other proteases may play a supplementary role. The direct comparison of T. rubrum grown on skin and nail medium showed different substrate favorite of secreted endoproteases. The genes MEP2, SUB5, SUB2 and SUB3 were more active during growth in skin medium, possibly these proteases have a higher affinity for skin original keratins. While the structures of SUB1, SUB4, and MEP4 maybe more suitable for the degradation of nail original keratins. This work presents useful molecular details for further understanding the pathogenesis of secreted proteases and the wide adaptation of T. rubrum.
authors.affiliation: Department of Dermatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 TianHe Road, TianHe District, Guangzhou 510630, P.R. China. chj75@medmail.com.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Jinling
authors.initials: J
authors.lastname: Yi
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Songchao
authors.initials: S
authors.lastname: Yin
authors.forename: Rongzhang
authors.initials: R
authors.lastname: Chen
authors.forename: Meirong
authors.initials: M
authors.lastname: Li
authors.forename: Congxiu
authors.initials: C
authors.lastname: Ye
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Lai
mesh_terms: D003001:Cloning, Molecular; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics; D004817:Epidermis / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D005810:Multigene Family; D009262:Nails / Q000737:chemistry; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity / Q000235:genetics; D014249:Trichophyton / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D037521:Virulence Factors; D007633:Keratins; D010447:Peptide Hydrolases; D005947:Glucose