Oxidative damage to DNA by 1,10-phenanthroline/L: -threonine copper (II) complexes with chlorogenic acid.
The oxidative DNA damage by copper (II) complexes in the presence of chlorogenic acid was explored using agarose gel electrophoresis. The extent of pBR322 DNA damage was enhanced significantly with increasing concentration of [Cu-phen-Thr] complex and incubation time. A fluorescence quenching activity of calf thymus DNA-EB was observed more remarkably with chlorogenic acid than without chlorogenic acid. The fluorescence measurements suggested that [Cu-phen-Thr] complex not only can bind to DNA by intercalation but also can damage the double strand DNA in the presence of chlorogenic acid. Further, 8-hydroxy-2'-deoxyguanosine, a biomarker of DNA oxidative damage was determined by electrochemical method. The control experiments revealed that the structure of copper (II) complexes affected capability of complex to DNA damage. The planar structure copper (II) complex showed high efficiency to DNA damage. The chlorogenic acid as biological reductant could improve copper (II) complex to DNA damage. A mechanism on [Cu-phen-Thr] complex to DNA damage in the presence of chlorogenic acid was proposed.
Wang
Yong
Y
College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China.
Zhang
Xiaoyan
X
Zhang
Qianru
Q
Yang
Zhousheng
Z
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002726:Chlorogenic Acid / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry*; D013912:Threonine / Q000737:chemistry*
D010618:Phenanthrolines; D013912:Threonine; D002726:Chlorogenic Acid; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; C427203:calf thymus DNA; D003849:Deoxyguanosine; C025205:1,10-phenanthroline