title: Cardioinhibitory properties of potato glycoalkaloids in beetles.
abstract: The semi-isolated heart bioassay was used to evaluate the effect of glycoalkaloids extracted from potato leaves on the heart contractile activity of three beetle species Zophobas atratus, Tenebrio molitor and Leptinotarsa decemlineata. The dose-response curves indicated species specific action of tested substances. Application of glycoalkaloids on the continuously perfused Z. atratus heart inhibited progressively frequency contractions; higher concentrations exerted short and reversible cardiac arrests. In the rest two beetle species tested glycoalkaloids caused no cardiotropic effect. In vivo bioassay with 1 day old Z. atratus pupae showed that the extract induces a negative inotropic effect on the heart.
authors.affiliation: Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89 Street, 61-614 Poznan, Poland. pmarcin@amu.edu.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Marciniak
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Adamski
authors.forename: Paweł
authors.initials: P
authors.lastname: Bednarz
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Slocinska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ziemnicki
authors.forename: Filomena
authors.initials: F
authors.lastname: Lelario
authors.forename: Laura
authors.initials: L
authors.lastname: Scrano
authors.forename: Sabino A
authors.initials: SA
authors.lastname: Bufo
mesh_terms: D000470:Alkaloids / Q000633:toxicity*; D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D009200:Myocardial Contraction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D011679:Pupa; D011198:Solanum tuberosum / Q000737:chemistry*; D013712:Tenebrio
chemical_list: D000470:Alkaloids; D007306:Insecticides; D010936:Plant Extracts