Antiangiogenic response after 70% hepatectomy and its relationship with hepatic regeneration and angiogenesis in rats.
BACKGROUND
The aim of this study was to evaluate the antiangiogenic response and its relation to regeneration and angiogenesis after 70% hepatectomy in a rat model.


METHODS
Sixty-four Wistar albino rats were included in the study. Animals were allocated into 8 groups (n = 8). After a 70% hepatectomy, liver regeneration, angiogenesis, and antiangiogenic response were evaluated in the remnant liver on days 0, 1, 2, 3, 5, 7, 10, and 14. Regeneration and angiogenesis were determined with immunoreactivity to proliferating cell nuclear antigen and vascular endothelial growth factor. Antiangiogenic response was evaluated by detecting collagen 18 m RNA with reverse transcriptase polymerase chain reaction.


RESULTS
We showed that liver regeneration peaked at day 1, whereas angiogenesis in the periportal and perisinusoidal areas reached their peak values on days 3 and 7, respectively. Both regeneration and angiogenic activity around perisinusoidal hepatocytes returned to basal activity on the day 10. Antiangiogenic response first appeared on day 5, reached a peak on day 10, and returned to basal values on day 14.


CONCLUSIONS
Collagen18 mRNA expression is present in the normal liver during the regenerative process. We suggest that the stimulus that causes the cessation of regeneration process may come from hepatocytes, and collagen 18 produced by hepatocytes may modulate this event by inhibiting the angiogenesis.
Dogrul
Ahmet Bulent
AB
Department of General Surgery, Medical School, Hacettepe University, Ankara, Turkey. ahmetdogrul@yahoo.com
Colakoglu
Tamer
T
Kosemehmetoglu
Kemal
K
Birben
Esra
E
Yaman
Elif
E
Gedikoglu
Gokhan
G
Abbasoglu
Osman
O
D020533:Angiogenesis Inhibitors / Q000378:metabolism; D000818:Animals; D043170:Collagen Type XVIII / Q000378:metabolism; D005260:Female; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000098:blood supply*; D008115:Liver Regeneration / Q000502:physiology*; D018919:Neovascularization, Physiologic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D020533:Angiogenesis Inhibitors; D043170:Collagen Type XVIII; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A