title: Use of liposomes to study vesicular transport.
abstract: Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
authors.affiliation: Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Takei
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Abe
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D002966:Clathrin / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis; D051381:Rats; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002966:Clathrin; D053835:Unilamellar Liposomes