title: A jugular bleeding technique in rabbits.
abstract: When studying pharmacokinetics in rabbits, researchers must often take multiple blood samples from conscious rabbits. Researchers usually collect these samples via the auricular vein, typically through a port or an indwelling catheter. The authors have developed an easy and efficient alternative method for obtaining multiple blood samples from conscious rabbits via the external jugular vein. This jugular bleeding technique serves as a refinement to blood sampling methods that require rabbits to undergo surgery (e.g., to insert a port) because it requires no alleviation of pain. During a 2-year period, the authors have taken multiple blood samples from more than 400 rabbits and have seen no adverse events attributed to this procedure.
authors.affiliation: Department of Laboratory Animal Resources, Merck and Co., Inc., West Point, PA, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Nelson
authors.forename: Gina L
authors.initials: GL
authors.lastname: Keller
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Mitchell
authors.forename: Brenda
authors.initials: B
authors.lastname: Pennypacker
authors.forename: Patricia
authors.initials: P
authors.lastname: Rebbeck
authors.forename: Irene T
authors.initials: IT
authors.lastname: Rogers
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D002408:Catheters, Indwelling; D007601:Jugular Veins / Q000033:anatomy & histology / Q000601:surgery*; D008866:Microsurgery / Q000662:veterinary; D011817:Rabbits; D012149:Restraint, Physical / Q000662:veterinary; D014656:Vascular Surgical Procedures / Q000379:methods*