title: Diagnostic utility of MOC-31, HBME-1 and MOC-31 mRNA in distinguishing between carcinoma cells and reactive mesothelial cells in pleural effusions.
abstract: OBJECTIVE
  To evaluate the individual and combined diagnostic utility of MOC-31, HBME-1 and MOC-31mRNA in the differentiation of adenocarcinoma cells from reactive mesothelial cells in pleural effusions.
  
  
  METHODS
  To subject the cells from 293 pleural effusions to immunocytochemical staining for MOC-31 and HBME-1 and reverse transcriptase polymerase chain reaction for the expression of MOC-31.
  
  
  RESULTS
  MOC-31 and MOC-31mRNA expression were significantly higher in the cancer cell group and in 3 subgrouped as adenocarcinoma, squamous cell carcinoma and small cell lung carcinoma than in the mesothelial cell group, whereas HBME-1 expression was obviously lower in those (p < 0.01). In single use, MOC-31mRNA had the highest sensitivity (90.8%) and accuracy (91.5%), whereas MOC-31 had the highest specificity (100%). When combinations of markers were evaluated together, MOC-31mRNA and HBME-1 gave a high diagnostic performance: sensitivity of 96.7% and accuracy of 95.2%, respectively.
  
  
  CONCLUSIONS
  The detection of MOC-31, HBME-1 and MOC-31-mRNA is helpful in distinguishing between cancer and reactive mesothelial cells in pleural effusions. MOC-31mRNA could be useful to the diagnosis of pleural micrometastasis.
authors.affiliation: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Wu
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Fang
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D010996:Pleural Effusion / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D000951:Antigens, Neoplasm
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C432845:HBME-1 antigen; D000951:Antigens, Neoplasm; C546130:MOC-31 monoclonal antibody, human