Using BCG, MPT-51 and Ag85 as antigens in an indirect ELISA for the diagnosis of bovine tuberculosis.
This study evaluated the Mycobacterium tuberculosis protein antigen MPT-51, the trimeric antigen 85 (Ag85) complex, and Bacillus Calmette-Guérin (BCG) in an indirect ELISA to diagnose bovine tuberculosis (TB) from serum samples. Serum was collected from 208 intra-dermal tuberculin test (ITT)-positive and 54 ITT-negative animals from a region where bovine TB is endemic. Using the Ag85 and BCG antigens, the indirect ELISA was able to discriminate ITT-positive from ITT-negative animals. This level of discrimination was not achieved when using the MPT-51 antigen. The highest sensitivity (Se) and specificity (Sp) of the test was found when BCG was used (Se, 82%; Sp, 91%). Further work in different epidemiological settings and with larger numbers of animals will be required to validate these findings.
da Silva
Ediane Batista
EB
Department of Microbiology, Immunology, Parasitology and Pathology, Research Center for Tropical Disease, Federal University of Goias, Rua Delenda Rezende de Melo, S/N, Setor Universitário, Goiânia, GO, Brazil.
Silva
Bruna Daniella de Souza
BD
Leon
Jerônimo Rafael Ruiz
JR
Kipnis
André
A
Santos
Isabel K F de Miranda
IK
Junqueira-Kipnis
Ana Paula
AP
D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D009163:Mycobacterium bovis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium; C065036:antigen 85, Mycobacterium bovis; C072681:Mycobacterium tuberculosis antigens