title: Single molecule imaging of protein molecules in nanopores.
abstract: The interactions between single protein molecules and nanoporous polycarbonate membranes were investigated at the single molecule level. Entrapment of proteins was shown to be size selective and was dependent on the membrane pore diameter. A pore size that is only slightly larger than the maximum dimension of the proteins was inadequate for intrusion into the pores. For a given protein, the number of molecules found at a given depth decreased as the pore size decreased. In addition, as the depth increased, for a given size pore, the number of molecules decreased rapidly. The depth-dependent histograms nicely fit a one-dimensional diffusion model. However, a highly restricted motion was observed even when the pore diameter was 10 times the size of the protein, resulting in anomalously small diffusion coefficients. We also demonstrated the subtle differences in depth distribution among BSA and hemoglobin that have nearly the same molecular weight but slightly different molecular shapes. These results give unique insights into the detailed mechanism of size-exclusion chromatography and membrane filtration.
authors.affiliation: Ames Laboratory-U.S. DOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Changbei
authors.initials: C
authors.lastname: Ma
authors.forename: Edward S
authors.initials: ES
authors.lastname: Yeung
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004058:Diffusion; D006454:Hemoglobins / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D011076:Polycarboxylate Cement; D011506:Proteins; C005506:polycarbonate; D012710:Serum Albumin, Bovine