title: Eukarya 18S rRNA gene diversity in the sea surface microlayer: implications for the structure of the neustonic microbial loop.
abstract: We have previously shown that there is a consistent and reproducible bacterioneuston community in the surface microlayer during a fjord mesocosm experiment. One possible cause of the surface microlayer-specific bacterial community is a surface microlayer-specific protist community selectively grazing on the bacterioneuston. We determined protist community structures using Eukarya 18S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent DGGE band sequencing using DNA samples that were collected from the surface microlayer and subsurface water of the mesocosms. As with bacterial communities, protist community structure was consistently different in the surface microlayer when compared with subsurface water. In particular, the protist community in the surface microlayer was dominated by Cercozoa, which were not detected in the subsurface water, and Ciliophora.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Cunliffe
authors.forename: J Colin
authors.initials: JC
authors.lastname: Murrell
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D056890:Eukaryota / Q000145:classification* / Q000235:genetics*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S