title: Topographically induced self-deformation of the nuclei of cells: dependence on cell type and proposed mechanisms.
abstract: Osteosarcoma-derived cell lines (SaOs-2, MG63) have recently been shown to deform their nucleus considerably in response to surface topography. Such a deformation had not been described previously. Here we present results on additional cell lines, including cancerous (OHS4, U2OS), immortalized (F/STRO-1(+)A and FHSO6) and healthy cells (HOP). The cancerous cells were found to deform extensively, the immortalized cells showed small deformations, whereas the healthy cells showed deformation only at short incubation times. These results suggest a strong link between the malignant transformation of cells and the state of the cytoskeletal network. We propose mechanisms to explain the deformation in which the cytoskeleton either pushes down on the nucleus during spreading or pulls it down upon adhesion to the pillars.
authors.affiliation: Institut de Science des Materiaux de Mulhouse, CNRS LRC 7228, Université de Haute Alsace, 68057, Mulhouse cedex, France.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Davidson
authors.forename: Olivia
authors.initials: O
authors.lastname: Fromigué
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Marie
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
authors.forename: Günter
authors.initials: G
authors.lastname: Reiter
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003584:Cytological Techniques; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D012516:Osteosarcoma / Q000378:metabolism; D010641:Phenotype; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)