title: Vascular endothelial growth factor polymorphisms in mantle cell lymphoma.
abstract: In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C-460T, C+936T and C-2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGFC-460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C-2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation.
authors.affiliation: Department of Oncology, Transplant, and Advances in Medicine Hematology Section, University of Pisa, Ospedale S. Chiara Via Roma 56, IT-56126 Pisa, Italy. s.galimberti @ med.unipi.it
authors.forename: S
authors.initials: S
authors.lastname: Galimberti
authors.forename: B
authors.initials: B
authors.lastname: Nagy
authors.forename: G A
authors.initials: GA
authors.lastname: Palumbo
authors.forename: E
authors.initials: E
authors.lastname: Ciancia
authors.forename: G
authors.initials: G
authors.lastname: Buda
authors.forename: E
authors.initials: E
authors.lastname: Orciuolo
authors.forename: A
authors.initials: A
authors.lastname: Melosi
authors.forename: P
authors.initials: P
authors.lastname: Lambelet
authors.forename: F
authors.initials: F
authors.lastname: Ronca
authors.forename: M
authors.initials: M
authors.lastname: Petrini
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A