title: Comparison of the receptor FGFRL1 from sea urchins and humans illustrates evolution of a zinc binding motif in the intracellular domain.
abstract: BACKGROUND
  FGFRL1, the gene for the fifth member of the fibroblast growth factor receptor (FGFR) family, is found in all vertebrates from fish to man and in the cephalochordate amphioxus. Since it does not occur in more distantly related invertebrates such as insects and nematodes, we have speculated that FGFRL1 might have evolved just before branching of the vertebrate lineage from the other invertebrates (Beyeler and Trueb, 2006).
  
  
  RESULTS
  We identified the gene for FGFRL1 also in the sea urchin Strongylocentrotus purpuratus and cloned its mRNA. The deduced amino acid sequence shares 62% sequence similarity with the human protein and shows conservation of all disulfides and N-linked carbohydrate attachment sites. Similar to the human protein, the S. purpuratus protein contains a histidine-rich motif at the C-terminus, but this motif is much shorter than the human counterpart. To analyze the function of the novel motif, recombinant fusion proteins were prepared in a bacterial expression system. The human fusion protein bound to nickel and zinc affinity columns, whereas the sea urchin protein barely interacted with such columns. Direct determination of metal ions by atomic absorption revealed 2.6 mole zinc/mole protein for human FGFRL1 and 1.7 mole zinc/mole protein for sea urchin FGFRL1.
  
  
  CONCLUSIONS
  The FGFRL1 gene has evolved much earlier than previously assumed. A comparison of the intracellular domain between sea urchin and human FGFRL1 provides interesting insights into the shaping of a novel zinc binding domain.
authors.affiliation: Department of Clinical Research, University of Bern, 3010 Bern, Switzerland. lei.zhuang@dkf.unibe.ch
authors.forename: Lei
authors.initials: L
authors.lastname: Zhuang
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Karotki
authors.forename: Philip
authors.initials: P
authors.lastname: Bruecker
authors.forename: Beat
authors.initials: B
authors.lastname: Trueb
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012333:RNA, Messenger / Q000378:metabolism; D051500:Receptor, Fibroblast Growth Factor, Type 5 / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012617:Sea Urchins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism
chemical_list: C417531:FGFRL1 protein, human; D012333:RNA, Messenger; D051500:Receptor, Fibroblast Growth Factor, Type 5; D011993:Recombinant Fusion Proteins; D015032:Zinc