Generation of gene-specific mutated rats using zinc-finger nucleases.
The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat genes directly in the embryo. ZFNs can specifically disrupt target genes in cultured rat cells and in embryos from inbred and outbred strains, leading to permanently genetically modified animals. This technology allows for the rapid, targeted modification of the rat genome.
Geurts
Aron M
AM
Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
Cost
Gregory J
GJ
Rémy
Séverine
S
Cui
Xiaoxia
X
Tesson
Laurent
L
Usal
Claire
C
Ménoret
Séverine
S
Jacob
Howard J
HJ
Anegon
Ignacio
I
Buelow
Roland
R
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002478:Cells, Cultured; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D004624:Embryo Transfer; D005260:Female; D055786:Gene Knockout Techniques / Q000379:methods; D005796:Genes; D016678:Genome; D008297:Male; D015202:Protein Engineering / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics*; D014158:Transcription, Genetic; D016335:Zinc Fingers
D012333:RNA, Messenger; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific