A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report.
OBJECTIVE
To determine whether CX(3)CR1(+) bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation.


RESULTS
CX(3)CR1(+) and CX(3)CR1(-) cells were isolated from marrow of CX(3)CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX(3)CR1(+) bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC.


CONCLUSIONS
CX(3)CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.
Metharom
Pat
P
Centre for Research in Vascular Biology, BioSciences Institute, University College Cork, College Road, Cork City, Ireland.
Kumar
Arun H S
AH
Weiss
Sharon
S
Caplice
Noel M
NM
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D020013:Calcium Signaling; D002454:Cell Differentiation; D002478:Cells, Cultured; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009000:Monocytes / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D019707:Receptors, Chemokine