title: Evaluation of anti-oxidant activities and total phenolic content of Chromolaena odorata.
abstract: The aim of this study was to assess the in vitro potential of chloroform extract of Chromolaena odorata leaves. The DPPH activity of the extract (0.1-5 mg/ml) was increased in a dose dependent manner, which was found in the range of 23.48-91.61% as compared to ascorbic acid (33.69-94.10%). The IC50 values of chloroform extract in DPPH radical, hydroxyl radical, nitric oxide, ABTS radical were obtained to be 0.31, 0.43, 0.28 and 1.32 mg/ml, respectively. However, the IC50 values for the standard ascorbic acid were noted to be 0.24, 0.41, 0.23 and 1 mg/ml, respectively. Measurement of total phenolic content of the chloroform extract of C. odorata was achieved using Folin-Ciocalteau reagent containing 242.2 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The results obtained in this study clearly indicate that C. odorata has a significant potential to use as a natural anti-oxidant agent.
authors.affiliation: Roland Institute of Pharmaceutical Sciences, Berhampur 760010, Orissa, India. ksrao108@gmail.com
authors.forename: K
authors.initials: K
authors.lastname: Srinivasa Rao
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Chaudhury
authors.forename: Anshuman
authors.initials: A
authors.lastname: Pradhan
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D036604:Chromolaena / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl