Chelating Langmuir-Blodgett film: a new versatile chemiluminescent sensing layer for biosensor applications.
The present study reports the achievement of a new chemiluminescent sensing layer able to simultaneously (i) play an active role on ligand immobilization and (ii) serve as a catalyst in detection processes for label-free biosensor applications. This new type of active Langmuir-Blodgett (LB) monolayer has been designed by using a chelating lipid (Ni-NTA-DOGS). Thanks to the chelated metallic cation, this peculiar lipid exhibits luminol chemiluminescence catalysis properties in the presence of hydrogen peroxide. Upon biomolecule interaction through imidazole ring chelation (mediated by the metallic cation bound to the lipid headgroup), the chemiluminescent signal can be modulated. The first chemiluminescent signal acquisition experiments have shown a strong and homogeneous signal of the chelating layer. Upon histamine interactions, a histidine derivative used as a marker of fresh food quality, we succeeded in obtaining as a proof of concept a chemiluminescent signal variation without any derivatization of the target molecule. This signal variation was shown to be directly correlated to the histamine concentration with a limit of detection of 2 microg/mL.
Santafé
Aurélie A-M
AA
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS, CPE Lyon, INSA de Lyon, UMR 5246, Université Lyon 1, Villeurbanne, F-69622, Lyon, France.
Blum
Loïc J
LJ
Marquette
Christophe A
CA
Girard-Egrot
Agnès P
AP
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D006632:Histamine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry; D036103:Nanotechnology; D009532:Nickel / Q000737:chemistry; D009829:Oleic Acids / Q000737:chemistry; D013386:Succinates / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D002614:Chelating Agents; D055767:Immobilized Proteins; C476561:N(alpha),N(alpha)-bis(carboxymethyl)-N(epsilon)-(1,2-dioleyl-sn-glycero-3-succinyl)-L-lysine; D009829:Oleic Acids; D013386:Succinates; D014867:Water; D008165:Luminol; D003300:Copper; D009532:Nickel; D006632:Histamine; D008239:Lysine