title: A single nucleotide polymorphism of chicken acetyl-CoA carboxylase A gene associated with fatness traits.
abstract: Acetyl-CoA carboxylase alpha (ACCalpha) is a major rate-limiting enzyme in the biogenesis of long-chain fatty acids. It can catalyze the carboxylation of acetyl-CoA to form malonyl-CoA that plays a key role in the regulation of fatty acid metabolism. The objective of the present study was to investigate the associations of ACCalpha gene polymorphisms with chicken growth and body composition traits. The Northeast Agricultural University broiler lines divergently selected for abdominal fat content and the Northeast Agricultural University F(2) Resource Population were used in the current study. Body weight and body composition traits were measured in the aforementioned two populations. A synonymous mutation was detected in the exon 19 region of ACCalpha gene, then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to genotype all the individuals derived from the aforementioned populations. Association analysis revealed that the polymorphism was associated with abdominal fat weight and percentage of abdominal fat in the two populations. The results suggested that ACCalpha gene could be a candidate locus or linked to a major gene that affects abdominal fat content in the chicken.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Tian
authors.forename: Shouzhi
authors.initials: S
authors.lastname: Wang
authors.forename: Qigui
authors.initials: Q
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Leng
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000235:genetics* / Q000502:physiology; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001835:Body Weight / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D056726:Genetic Association Studies; D005838:Genotype; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D000103:Acetyl-CoA Carboxylase