alpha1,2-Fucosylated and beta-N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer.
A prostate-specific antigen (PSA) is widely used as a diagnostic marker for prostate cancer (PC) because of its high specificity. However, elevated serum PSA does not occur only in PC but also in benign prostatic hyperplasia (BPH). Since the structural changes of N-glycans during carcinogenesis are common phenomena, we investigated whether PC-specific N-glycans are linked to PSA. We first analyzed the carbohydrate structures of PSA derived from seminal fluid, serum of BPH and PC patients, and PC cell line, namely, LNCaP using eight lectin-immobilized columns and then with enzyme-linked immunosorbent assay (ELISA). The fraction of serum PSA from PC patients bound to both Fucalpha1-2Gal and betaGalNAc binding Trichosanthes japonica agglutinin-II (TJA-II) column, while that from BPH patients did not exhibit this binding ability, thereby implying that there is elevated expression of alpha1,2-fucosylation and beta-N-acetylgalactosaminylation of PSA during carcinogenesis. We then performed a real-time polymerase chain reaction (PCR) and confirmed that these structural changes were responsible for the elevated expression of fucosyltransferase I (FUT1) and beta-N-acetylgalactosaminyltransferase 4(B4GALNT4). Second, we measured TJA-II-bound PSA contents and the binding ratios of TJA-II column chromatography in serum PSA samples from 40 patients of both PC and BPH. The results indicated that both TJA-II-bound PSA content and TJA-II binding ratios (%) could be used to discriminate between PC and BPH with more than 95% probability, and TJA-II-bound PSA can be regarded as a potential marker of PC.
Fukushima
Keiko
K
Innovative Research Initiatives, Tokyo Institute of Technology, Yokohama 226-8501.
Satoh
Takefumi
T
Baba
Shiro
S
Yamashita
Katsuko
K
D015415:Biomarkers / Q000097:blood; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008297:Male; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011134:Polysaccharides; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000737:chemistry* / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D012661:Semen / Q000378:metabolism; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D015415:Biomarkers; D037102:Lectins; D017350:N-Acetylgalactosaminyltransferases; D011134:Polysaccharides; D017430:Prostate-Specific Antigen; D000097763:Polypeptide N-acetylgalactosaminyltransferase