Experimental splenectomies and malaria in mice.
OBJECTIVE
To evaluate the importance of spleen in malaric infection in murino model, comparing the parasitemia and the titles of immunoglobulins in the different groups.


METHODS
It was used female mice non-isogenic, inoculated with Plasmodium berghei, cepa ANKA, intraperitoneally. The parasitemia was analyzed in 23rd, 25th, 27th and 32nd day of the experiment, being the stained blood exam colored by Giemsa. The titles of the total serum immunoglobulins IgM and IgG were analyzed by Dot-ELISA technique, at 6th, 22nd and 32nd day, when the animals were sacrificed.


RESULTS
The parasitemia was gradual in all the inoculated groups. In the end of the experiment, the animals with partial parasitemia present superior parasitemia, but next to the non-splenectomized, while the asplenics present difference bigger than the double. The levels of total serum IgM and IgG didn't have significant changes with the removal partial or total splenic.


CONCLUSIONS
The techniques conservatives in splenic trauma are possible and necessary. The importance of remaining spleen in the clearance of red blood cells parasitized by Plasmodium berghei showed being efficient, in order to avoid serious complications resulting of the malaria in mice.
Negreiros
Róbson Miguel de Araújo
RM
Surgery Clinical Department, School of Medicine, UFAM, R. Afonso Pena 1053, Manaus, Amazonas, Brazil. negreirosrobson@hotmail.com
Makimoto
Fabiano Hiromichi
FH
Santana
Linda Luciana Oliveira
LL
Ferreira
Luís Carlos de Lima
LC
Nakajima
Gerson Suguiyama
GS
Santos
Maria Cristina dos
MC
D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008288:Malaria / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D051379:Mice; D018512:Parasitemia / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D010962:Plasmodium berghei / Q000276:immunology*; D011446:Prospective Studies; D011897:Random Allocation; D013156:Splenectomy / Q000009:adverse effects / Q000379:methods*
D007074:Immunoglobulin G; D007075:Immunoglobulin M