title: Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis.
abstract: Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
authors.affiliation: Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
authors.forename: Carlos A N
authors.initials: CA
authors.lastname: Ramos
authors.forename: Flábio R
authors.initials: FR
authors.lastname: Araújo
authors.forename: Ingrid I F
authors.initials: II
authors.lastname: Souza
authors.forename: Renato H M
authors.initials: RH
authors.lastname: Oliveira
authors.forename: Carina
authors.initials: C
authors.lastname: Elisei
authors.forename: Cleber O
authors.initials: CO
authors.lastname: Soares
authors.forename: Ana M S
authors.initials: AM
authors.lastname: Sacco
authors.forename: Grácia M S
authors.initials: GM
authors.lastname: Rosinha
authors.forename: Leucio C
authors.initials: LC
authors.lastname: Alves
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000097:blood*; D016793:Babesia bovis / Q000276:immunology* / Q000302:isolation & purification; D001404:Babesiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology; D007074:Immunoglobulin G / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0