Rotavirus infection activates dendritic cells from Peyer's patches in adult mice.
This study used an in vivo mouse model to analyze the response of dendritic cells (DCs) in Peyer's patches (PPs) within the first 48 h of infection with the wild-type murine rotavirus EDIM (EDIM(wt)). After the infection, the absolute number of DCs was increased by 2-fold in the PPs without a modification of their relative percentage of the total cell number. Also, the DCs from PPs of infected mice showed a time-dependent migration to the subepithelial dome (SED) and an increase of the surface activation markers CD40, CD80, and CD86. This response was more evident at 48 h postinfection (p.i.) and depended on viral replication, since DCs from PPs of mice inoculated with UV-treated virus did not show this phenotype. As a result of the activation, the DCs showed an increase in the expression of mRNA for the proinflammatory cytokines interleukin-12/23p40 (IL-12/23p40), tumor necrosis factor alpha (TNF-alpha), and beta interferon (IFN-beta), as well as for the regulatory cytokine IL-10. These results suggest that, a short time after rotavirus infection, the DCs from PPs play a critical role in controlling the infection and, at the same time, avoiding an excessive inflammatory immune response.
Lopez-Guerrero
Delia V
DV
Facultad de Medicina, Universidad Autonoma del Estado de Morelos, 62210 Morelos, Mexico.
Meza-Perez
Selene
S
Ramirez-Pliego
Oscar
O
Santana-Calderon
Maria A
MA
Espino-Solis
Pavel
P
Gutierrez-Xicotencatl
Lourdes
L
Flores-Romo
Leopoldo
L
Esquivel-Guadarrama
Fernando R
FR
D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001483:Base Sequence; D019013:CD40 Antigens / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012400:Rotavirus Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D012333:RNA, Messenger