title: Quantitative neuroproteomics of the synapse.
abstract: An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.
authors.affiliation: Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY, USA.
authors.forename: Dinah Lee
authors.initials: DL
authors.lastname: Ramos-Ortolaza
authors.forename: Ittai
authors.initials: I
authors.lastname: Bushlin
authors.forename: Noura
authors.initials: N
authors.lastname: Abul-Husn
authors.forename: Suresh P
authors.initials: SP
authors.lastname: Annangudi
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sweedler
authors.forename: Lakshmi A
authors.initials: LA
authors.lastname: Devi
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012984:Software; D013569:Synapses / Q000737:chemistry*
chemical_list: D009419:Nerve Tissue Proteins