Developmental regulation of the pheromone biosynthesis activating neuropeptide-receptor (PBAN-R): re-evaluating the role of juvenile hormone.
Sex pheromone production in Helicoverpa armigera is regulated by pheromone-biosynthesis-activating neuropeptide (PBAN), which binds to a G-protein coupled receptor at the pheromone gland. We demonstrate the temporal differential expression levels of the PBAN receptor (PBAN-R) gene, reaching peak levels at a critical period of 5 h post-eclosion. Previous studies implied a possible regulatory role for juvenile hormone (JH). We herein demonstrate that PBAN-R expression levels increase normally when females are decapitated or head-ligated, removing the source of JH, before peak transcript levels are reached. Similarly, sex pheromone production can be induced by PBAN in such decapitated females. These results indicate that up-regulation, at this critical time, is not dependent on JH originating from the head. Conversely, JH injected in vivo at this critical period significantly inhibits PBAN-R transcript levels.
Bober
R
R
Department of Entomology, The Hebrew University, Rehovot, Israel.
Azrielli
A
A
Rafaeli
A
A
D000818:Animals; D049248:Decapitation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000378:metabolism*; D007306:Insecticides; D007605:Juvenile Hormones / Q000378:metabolism*; D008297:Male; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D048448:Phenylcarbamates; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012724:Sex Attractants / Q000096:biosynthesis*
D019476:Insect Proteins; D007306:Insecticides; D007605:Juvenile Hormones; D009479:Neuropeptides; D048448:Phenylcarbamates; D043562:Receptors, G-Protein-Coupled; D012724:Sex Attractants; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; C052034:fenoxycarb