Investigation of the sequence and length dependence for cell-penetrating prenylated peptides.
Cell penetrating peptides are useful delivery tools for introducing molecules of interest into cells. A new class of cell penetrating molecules has been recently reported-cell penetrating, prenylated peptides. In this study a series of such peptides was synthesized to examine the relationship between peptide sequence and level of peptide internalization and to probe their mechanism of internalization. This study revealed that prenylated peptides internalize via a non-endocytotic pathway regardless of sequence. Sequence length and identity was found to play a role in peptide uptake but prenylated sequences as short as two amino acids were found to exhibit significant cell penetrating properties.
Wollack
James W
JW
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
Zeliadt
Nicholette A
NA
Ochocki
Joshua D
JD
Mullen
Daniel G
DG
Barany
George
G
Wattenberg
Elizabeth V
EV
Distefano
Mark D
MD
D000595:Amino Acid Sequence; D002463:Cell Membrane Permeability / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017368:Protein Prenylation
D005456:Fluorescent Dyes; D010455:Peptides