Liposome biodistribution via europium complexes.
The drug-delivery field needs tools to follow vector biodistribution. Radioactive tracers and conventional fluorophores are widely used. We propose here to use europium complexes. Use of pulsed light source time-resolved fluorimetry takes into account the fluorescence decay time of the lanthanide chelates to gain sensitivity in biological media. The method was developed to follow liposome biodistribution. Octadecyl-DTPA.Eu compound has been prepared and incorporated into liposomes without alteration of its fluorescence signal. The method has been validated by comparison with fluorophore-labelled liposomes. The way to proceed to use this method for liposomes or other vectors is detailed.
Mignet
Nathalie
N
Unité de Pharmacologie Chimique et Génétique, CNRS, UMR, Paris, France.
Scherman
Daniel
D
D000818:Animals; D002614:Chelating Agents; D005063:Europium / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D008081:Liposomes / Q000032:analysis / Q000493:pharmacokinetics*; D051379:Mice; D008810:Mice, Inbred C57BL
D002614:Chelating Agents; D008081:Liposomes; D005063:Europium