Enhanced production of 6-aminopenicillanic acid in aqueous methyl isobutyl ketone system with immobilized penicillin G acylase.
Enzymatic hydrolysis of penicillin G for production of 6-amino-penicillanic-acid (6-APA) was achieved by using penicillin G acylase as catalyst in an aqueous-methylisobutyl ketone (MIBK) system. The optimization was carried out and it was found that the best conversion was improved 10% more than the aqueous system, which was obtained at the conditions: initial pH 8.0, 5.0% (W/V) substrate (penicillin G), and temperature at 35 degrees C, and the ratio of aqueous and organic phase was 3:1. The stability of the biocatalyst was studied at the operational conditions. After 5 cycles of semi-batch reactions, the residual activity of penicillin G acylase was 69.2% of the initial activity. There was no apparent loss of the yield of product. This process has a potential application in the industrial scale production of 6-APA because it simplifies the process effectively.
Fang
Shu-Guang
SG
School of Pharmacy, Jiangsu University, Zhenjiang, PR China.
Qiang
Tan
T
Liu
Rui-Jiang
RJ
Xu
Xi-Ming
XM
Zhang
Ye-Wang
YW
D001410:Bacillus megaterium / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006868:Hydrolysis; D008742:Methyl n-Butyl Ketone / Q000737:chemistry; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D010399:Penicillin Amidase / Q000378:metabolism*; D010400:Penicillin G / Q000378:metabolism; D014867:Water / Q000737:chemistry
D004800:Enzymes, Immobilized; D014867:Water; D008742:Methyl n-Butyl Ketone; D010397:Penicillanic Acid; D010399:Penicillin Amidase; D010400:Penicillin G; C004408:aminopenicillanic acid; C005458:methyl isobutyl ketone