Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection.
To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE recognition event involves competition between the molecular beacon and loose HNE aptamer for the binding the short DNA strand. The new biosensor can detect as little as 0.34nM of HNE, and the response is linear in the tested concentration range of 0.34-68nM with the detection limit of 47pM.
He
Jing-Lin
JL
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Wu
Zai-Sheng
ZS
Zhang
Song-Bai
SB
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis* / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide; D012996:Solutions; D004247:DNA; D019272:Leukocyte Elastase; D019793:Fluorescein