RON-expressing MCF-10A breast epithelial cells exhibit alterations of hyaluronan expression, promoting RON-mediated early adhesion events.
The receptor tyrosine kinase known as RON appears to play a role in the progression of human carcinomas, and is associated with a poor patient prognosis. Our current study demonstrates that RON expression in MCF-10A breast epithelial cells lead to an alteration of cell-surface hyaluronan compared to the parental cells. We found that hyaluronan was important for initial cell attachment to poly-d-lysine-coated coverslips, but did not contribute to the process of cell spreading. Previous data implied that the Src kinase was important for spreading but not the initial attachment of 10A cells, and here we demonstrate Src activation was also not necessary for hyaluronan production in these cells.
Feres
Kimberly J
KJ
Stony Brook University, Department of Molecular Genetic & Microbiology, 130 Life Sciences Building, Stony Brook, NY 11794-5222, USA. Kimberly.Gottfredsen@stonybrook.edu
Hayman
Michael J
MJ
D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*
D006820:Hyaluronic Acid; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases