title: Anti-inflammatory evaluation of Coronopus didymus in the pleurisy and paw oedema models in mice.
abstract: BACKGROUND
  Mastrunço (Coronopus didymus--CD) is currently considered as a medicinal specie often used in Brazil, especially in southeast region, for the treatment of several diseases in which pain and inflammation are common. Treatment with the plant can be done by infusion, decoction, or through food. The aim of this study was: to investigate the anti-inflammatory effect of hydroalcoholic extract obtained from the leaves of CD following the traditional procedure.
  
  
  METHODS
  The anti-inflammatory activity was determined using mouse of pleurisy and paw oedema models, both process being induced by different flogistic agents such as: carrageenan (Cg), bradykinin (BK), histamine (HIS), substance P (SP), dextran (DEX) or prostaglandin E(2) (PGE(2)). We evaluated the effect of CD (200-600 mg/kg) administered by oral route (p.o.) upon leukocytes migration, myeloperoxidase (MPO), and adenosine-deaminase (ADA) activities and nitric oxide (NO) levels.
  
  
  RESULTS
  CD (200-600 mg/kg) inhibited the leukocytes by 60.0+/-1.42%, neutrophils by 82.75+/-1.29%, MPO by 42.30+/-4.23%, and ADA activities by 57.89+/-1.94%, as well as NO levels by 64.28+/-2.15% in Cg induced pleurisy. CD also inhibited total and differential leukocytes in the pleurisy induced by BK (1.30+/-0.11/0.29+/-0.02), HIS (1.20+/-0.09/0.42+/-0.05) and SP (0.74+/-0.06/0.14+/-0.01). In addition, CD was effective in reducing paw oedema induced by Cg by 72.79+/-1.13%, SP by 68.26.+/-0.78%, BK by 66.66.+/-0.77%, PGE(2) by 53.346.+/-1.18 and DEX by 65.14+/-2.35%.
  
  
  CONCLUSIONS
  Several mechanisms, including the inhibition of enzymes (MPO and ADA) and mediators (BK, HIS, SP, NO and PGE(2)) release and/or action, appear to account for the anti-inflammatory effect of Coronopus didymus.
authors.affiliation: Centro de Ciências da Saúde, CCS, Mestrado em Ciências Farmacêuticas Universidade do Vale do Itajaí, UNIVALI. Itajaí, SC, Brazil.
authors.forename: Tereza Cristina Pamplona Mosimann
authors.initials: TC
authors.lastname: Busnardo
authors.forename: Cristina
authors.initials: C
authors.lastname: Padoani
authors.forename: Ticiana Camila
authors.initials: TC
authors.lastname: Mora
authors.forename: Maique Weber
authors.initials: MW
authors.lastname: Biavatti
authors.forename: Tânia Silvia
authors.initials: TS
authors.lastname: Fröde
authors.forename: Cristiani
authors.initials: C
authors.lastname: Bürger
authors.forename: Vanessa D
authors.initials: VD
authors.lastname: Claudino
authors.forename: Eduardo Monguilhote
authors.initials: EM
authors.lastname: Dalmarco
authors.forename: Márcia Maria
authors.initials: MM
authors.lastname: de Souza
mesh_terms: D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001920:Bradykinin / Q000494:pharmacology; D001938:Brazil; D002351:Carrageenan / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D006632:Histamine / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D009504:Neutrophils / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology; D009195:Peroxidase / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D013373:Substance P / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D009569:Nitric Oxide; D013373:Substance P; D006632:Histamine; D002351:Carrageenan; D009195:Peroxidase; D000243:Adenosine Deaminase; D001920:Bradykinin