Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.
A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal antibody was found to recognize a sequence of GREGEQEWGTPGSHVREETS. Further analysis with shorter pin-peptides with eight amino acid-long showed that the sequence of QEWGTPGS was an essential linear sequence of this epitope. When the QEW part of the sequence was replaced by alanine, the 92-2 monoclonal antibody did not bind to the substituted peptide, showing that those amino acids play an important role in the binding of the 92-2 monoclonal antibody.
Shinmoto
Hiroshi
H
Tamagawa University, Machida, Tokyo, 194-8610, Japan, shnmt920@agr.tamagawa.ac.jp.
Matsuo
Yuji
Y
Naganawa
Yasunori
Y
Tomita
Shinichi
S
Takano-Ishikawa
Yuko
Y