Loss of phosphomannomutase activity enhances actinorhodin production in Streptomyces coelicolor.
Phosphomannomutase (ManB), whose main function is the conversion of mannose-6-phosphate to mannose-1-phosphate, is involved in biosynthesis of GDP-mannose for numerous processes such as synthesis of structural carbohydrates, production of alginates and ascorbic acid, and post-translational modification of proteins in prokaryotes and eukaryotes. ManB isolated from Streptomyces coelicolor was shown to have both phosphomannomutase and phosphoglucomutase activities. Deletion of manB in S. coelicolor caused a dramatic increase in actinorhodin (ACT) production in the low-glucose Difco nutrient (DN) medium, whereas the wild-type strain did not produce ACT on this medium. Experiments involving complementation of the manB deletion showed that increased ACT production in DN media was due to blockage of phosphomannomutase activity rather than phosphoglucomutase activity. This result therefore provides useful information for the design of strategies that enhance antibiotic production through the control of carbon flux.
Yang
Yung-Hun
YH
School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Song
Eunjung
E
Park
Sung-Hee
SH
Kim
Ji-Nu
JN
Lee
Kwangwon
K
Kim
Eunjung
E
Kim
Yun-Gon
YG
Kim
Byung-Gee
BG
D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006003:Glycogen / Q000096:biosynthesis; D016297:Mutagenesis, Site-Directed; D010733:Phosphoglucomutase / Q000235:genetics / Q000378:metabolism; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002244:Carbon; D006003:Glycogen; D010733:Phosphoglucomutase; D017875:Phosphotransferases (Phosphomutases); C052799:phosphomannomutase; C013390:actinorhodin; D000095622:Benzoisochromanequinones