Cloning and characterization of the first amphibian bradykinin gene.
More than ten bradykinin-related peptides and their cDNAs have been identified from amphibians, but their genes are unknown. In present study, four cDNAs encoding one, two, four and six copies of bradykinin-related peptides were cloned from the frog (Odorrana grahami) skin cDNA library, respectively. Three bradykinin-related peptides (bradykinin, Thr6-bradykinin, Leu5Thr6-bradykinin) were deduced from these four cDNA sequences. Based on the cDNA sequence, the gene sequence encoding an amphibian bradykinin-related peptide from O. grahami was determined. It is composed of 7481 base pairs including two exons and two introns. The first exon codes signal peptide and the second exon codes acidic spacer peptide and Thr6-bradykinin. The promoter region of the bradykinin gene contains several putative recognition sites for nuclear factors, such as SRY, GATA-1, LYF-1, DeltaE, CDXA, NKX-2.5, MIF1 and S8. The current work may facilitate to understand the regulation and possible functions of amphibian skin bradykinin-related peptides.
Chen
Manda
M
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, China.
Che
Qiaolin
Q
Wang
Xu
X
Li
Jianxu
J
Yang
Hailong
H
Li
Dongsheng
D
Zhang
Keyun
K
Lai
Ren
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001920:Bradykinin / Q000235:genetics* / Q000494:pharmacology; D003001:Cloning, Molecular; D005091:Exons; D005260:Female; D015723:Gene Library; D006168:Guinea Pigs; D007082:Ileum / Q000033:anatomy & histology / Q000187:drug effects; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010455:Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D011898:Ranidae / Q000235:genetics*; D016415:Sequence Alignment
D010455:Peptides; D001920:Bradykinin