title: Acute effects of chlorpyryphos-ethyl and secondary treated effluents on acetylcholinesterase and butyrylcholinesterase activities in Carcinus maenas.
abstract: The acute effects of commercial formulation of chlorpyrifos-ethyl (Dursban) and the secondary treated industrial/urban effluent (STIUE) exposure on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities in hepatopancreas and gills of Mediterranean crab Carcinus maenas were investigated. After 2 d of exposure to chlorpyriphos-ethyl, the AChE activity was inhibited in both organs at concentrations of 3.12 and 7.82 microg/L, whereas the BuChE was inhibited only at higher concentration 7.82 microg/L of commercial preparation Dursban. The exposure of crabs to Dursban (3.12 microg/L) showed a significant decrement of AChE activity at 24 and 48 h, whereas the BuChE was inhibited only after 24 h and no inhibition for both enzymes was observed after 72 h. Moreover, a significant repression of AChE activity was observed in both organs of C. maenas exposed to 5% of STIUE. Our experiments indicated that the measurement of AChE activity in gills and hepatopancreas of C. maenas would be useful biomarker of organophosphorous (OP) and of neurotoxic effects of STIUE in Tunisia.
authors.affiliation: Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042 Sousse, Tunisia. ghedira_jihene@yahoo.fr
authors.forename: Jihene
authors.initials: J
authors.lastname: Ghedira
authors.forename: Jamel
authors.initials: J
authors.lastname: Jebali
authors.forename: Zied
authors.initials: Z
authors.lastname: Bouraoui
authors.forename: Mohamed
authors.initials: M
authors.lastname: Banni
authors.forename: Lassaad
authors.initials: L
authors.lastname: Chouba
authors.forename: Hamadi
authors.initials: H
authors.lastname: Boussetta
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D003386:Brachyura / Q000187:drug effects* / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000378:metabolism*; D004390:Chlorpyrifos; D004789:Enzyme Activation / Q000187:drug effects; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D009946:Organothiophosphorus Compounds; D014874:Water Pollutants, Chemical; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D004390:Chlorpyrifos