title: Verification of biomarkers for diabetic retinopathy by multiple reaction monitoring.
abstract: Multiple reaction monitoring was used to verify target proteins in 3 groups of vitreous and plasma samples from 3 stages of diabetic retinopathy: macular hole (nondiabetic control), nonproliferative diabetic retinopathy, and proliferative diabetic retinopathy. Twelve target proteins were quantified using triple quadrupole LC-MS/MS and 3 methods to determine the transitions (information-dependent analysis, the MIDAS workflow, and the PeptideAtlas database). This study might be the first MRM experiment to analyze large numbers of clinical vitreous and plasma samples for biomarker verification. Consequently, several biomarker candidates were identified for use in further applications.
authors.affiliation: Department of Biomedical Sciences, Cancer Research Institute, and Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
authors.forename: Kyunggon
authors.initials: K
authors.lastname: Kim
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyeong Gon
authors.initials: HG
authors.lastname: Yu
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Yu
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
authors.forename: In-Jin
authors.initials: IJ
authors.lastname: Jang
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002853:Chromatography, Liquid; D003930:Diabetic Retinopathy / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers