Encapsulation of ascorbyl palmitate in chitosan nanoparticles by oil-in-water emulsion and ionic gelation processes.
The encapsulation of ascorbyl palmitate (AP) in chitosan particles was carried out by droplet formation via an oil-in-water emulsion, followed by droplet solidification via ionic gelation using sodium triphosphate pentabasic (TPP) as a cross-linking agent. The success of AP encapsulation was confirmed by FT-IR, UV-vis spectrophotometry, TGA, and XRD techniques. The obtained AP-loaded chitosan particles were spherical in shape with an average diameter of 30-100nm as observed by SEM and TEM. Loading capacity (LC) and encapsulation efficiency (EE) of AP in the nanoparticles were about 8-20% and 39-77%, respectively, when the initial AP concentration was in the range of 25-150% (w/w) of chitosan. Augmentation of the initial AP concentration led to an increase of LC and a reduction of EE. The amount of AP released from the nanoparticles in ethanol and tris buffer (pH approximately 8.0) increased with increasing LC and decreasing TPP concentration.
Yoksan
Rangrong
R
Department of Packaging and Materials Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand. rangrong.y@ku.ac.th
Jirawutthiwongchai
Jatesuda
J
Arpo
Kridsada
K
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D002214:Capsules / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D007477:Ions; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009821:Oils / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry*
D002214:Capsules; D004655:Emulsions; D005782:Gels; D007477:Ions; D009821:Oils; D014867:Water; D048271:Chitosan; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid