title: [Gene profiling after knocking-down the expression of NS gene in prostate cancer PC-3 cells].
abstract: OBJECTIVE
  To screen the genes and possible signal transduction pathways involved in the mechanism of nucleostemin (NS) in the proliferation of prostate cancer.
  
  
  METHODS
  Oligonucleotide DNA microarray was used to screen the genome changes after knocking-down expression of NS in PC-3 cells and quantitative real-time PCR was used to further confirm the important differentially expressed genes.
  
  
  RESULTS
  219 differentially expressed genes were found and theses genes were involved in cell cycle, cell proliferation, signal transduction, cell apoptosis and cell differentiation, etc. INK4 family genes (p15, p16, p18) were up-regulated and cyclin D1, HDAC1 were down-regulated, the main action points were CDK4/6-cyclin D and pRb-E2F1 complexes.
  
  
  CONCLUSIONS
  NS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. NS is an important G(1)/S checkpoint regulator and its regulatory activity has been certified at gene level.
authors.affiliation: Department of Urology, Second Hospital, Tianjin Medical University & Tianjin Institute of Urology, Tianjin 300211, China.
authors.forename: Ran-lu
authors.initials: RL
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Jian-tao
authors.initials: JT
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sheng-zhi
authors.initials: SZ
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D056284:Histone Deacetylase 1 / Q000378:metabolism; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D015398:Signal Transduction
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D019938:Cyclin D1; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D019204:GTP-Binding Proteins