title: Physiological versatility of the extremely thermoacidophilic archaeon Metallosphaera sedula supported by transcriptomic analysis of heterotrophic, autotrophic, and mixotrophic growth.
abstract: Comparative transcriptomic analysis of autotrophic, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula (70 degrees C, pH 2.0) revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-hydroxybutyrate pathway and implicated a membrane-bound hydrogenase (Msed_0944-Msed_0946) for growth on H(2). Routes for generation of ATP and reducing equivalents were also identified.
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
authors.forename: Kathryne S
authors.initials: KS
authors.lastname: Auernik
authors.forename: Robert M
authors.initials: RM
authors.lastname: Kelly
mesh_terms: D000332:Aerobiosis / Q000235:genetics; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D052818:Autotrophic Processes; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D020869:Gene Expression Profiling; D019848:Gene Expression Regulation, Archaeal; D052836:Heterotrophic Processes; D006358:Hot Temperature; D006885:Hydroxybutyrates / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013379:Substrate Specificity / Q000235:genetics; D016943:Sulfolobaceae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D002247:Carbon Isotopes; D006885:Hydroxybutyrates; D002245:Carbon Dioxide