Delivery of cloned offspring: experience in Zebu cattle (Bos indicus).
The production of a healthy cloned calf is dependent on a multitude of successful steps, including reprogramming mediated by the oocyte, the development of a functional placenta, adequate maternal-fetal interaction, the establishment of a physiological metabolic setting and the formation of a complete set of well-differentiated cells that will eventually result in well-characterised and fully competent tissues and organs. Although the efficiency of nuclear transfer has improved significantly since the first report of a somatic cell nuclear transfer-derived animal, there are many descriptions of anomalies concerning cloned calves leading to high perinatal morbidity and mortality. The present article discusses some our experience regarding perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has led to improved survival rates in Nellore cloned calves following the application of such 'labour-intensive technology'.
Meirelles
Flávio V
FV
Faculty of Animal Sciences and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225. Pirassununga, SP 13635-900, Brazil. meirellf@usp.br
Birgel
Eduardo H
EH
Perecin
Felipe
F
Bertolini
Marcelo
M
Traldi
Anneliese S
AS
Pimentel
José Rodrigo V
JR
Komninou
Eliza R
ER
Sangalli
Juliano R
JR
Neto
Paulo Fantinato
PF
Nunes
Mariana Tikuma
MT
Pogliani
Fábio Celidonio
FC
Meirelles
Flávia D P
FD
Kubrusly
Flávia S
FS
Vannucchi
Camila I
CI
Silva
Liege C G
LC
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle / Q000196:embryology*; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D000013:Congenital Abnormalities / Q000401:mortality / Q000662:veterinary; D005260:Female; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D015996:Survival Rate