Protein-resistant polymer coatings based on surface-adsorbed poly(aminoethyl methacrylate)/poly(ethylene glycol) copolymers.
We report on the protein-resistant properties of glass substrates coated with novel copolymers of 2-aminoethyl methacrylate hydrochloride and poly(ethylene glycol) methyl ether methacrylate (AEM-PEG). In comparison to currently available protein-blocking polymer systems, such as poly-l-lysine-poly(ethylene glycol), silane-based poly(ethylene glycol), and poly(ethylene glycol) brushes prepared by surface-initiated polymerization, the proposed AEM-PEG offers the combined advantages of low cost, simplicity of use, and applicability in aqueous solutions. We demonstrate the capability of AEM-PEG to block the surface binding of globular proteins (tubulin), their assemblies (microtubules), and functional motor proteins (kinesin-1). Moreover, we demonstrate the applicability of AEM-PEG for surface patterning of proteins in microfluidic devices.
Ionov
Leonid
L
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauer Str. 108, 01307 Dresden, Germany. ionov@ipfdd.de; ionov@mpi-cbg.de
Synytska
Alla
A
Kaul
Elisabeth
E
Diez
Stefan
S
D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012821:Silanes / Q000737:chemistry*; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
D008689:Methacrylates; D011108:Polymers; D012821:Silanes; D014404:Tubulin; C490327:poly(ethylene glycol)silane; D011092:Polyethylene Glycols; C072703:2-aminoethylmethacrylate; D016547:Kinesins