13C labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae.
The structure of zeamine, a novel polyamino-amide antibiotic metabolite of Dickeya zeae has been established by NMR and detailed MS analyses; labelling studies with (13)C-labelled acetates suggest that the repeating secondary amine containing motif may be biosynthesised via a modular PKS containing aminotransferase domains.
Wu
Jien
J
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673. jwu@imcb.a-star.edu.sg
Zhang
Hai-Bao
HB
Xu
Jin-Ling
JL
Cox
Russell J
RJ
Simpson
Thomas J
TJ
Zhang
Lian-Hui
LH
D000586:Amination; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry; D004755:Enterobacteriaceae / Q000201:enzymology*; D018942:Macrolides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D018942:Macrolides; D011073:Polyamines; C547236:zeamine; D048630:Polyketide Synthases