title: Immunity-related gene single nucleotide polymorphisms associated with Rhodococcus equi infection in foals.
abstract: In previous work, we found significant associations of horse polymorphic microsatellite and immunity-related (IR) gene markers with Rhodococcus equi infection of foals. Here, a statistically significant association between a single nucleotide polymorphism (SNP) within the interleukin 7 receptor-encoding gene (IL7R) with high R. equi burden in transtracheal aspirates was found (Fisher's F = 0.043, odds ratio: 8.00, 95% confidence interval: 1.127-56.795). Further positional and/or functional candidate genes investigated TLR2, IL13, IL17A, IL28R, TACE/ADAM 17 and GBP1, were not associated with infection in this study. SNPs analysed were found by sequencing and appropriate restriction fragment length polymorphism markers were developed. Their associations with R. equi infection were tested by genotyping thoroughbred foals from the original study. The association was confirmed by analysing genotypes composed with genes previously reported to be associated with R. equi infection in the same group.
authors.affiliation: Institute of Animal Genetics, Faculty of Veterinary Medicine, Brno 612 42, Czech Republic. horinp@vfu.cz
authors.forename: P
authors.initials: P
authors.lastname: Horin
authors.forename: K
authors.initials: K
authors.lastname: Sabakova
authors.forename: J
authors.initials: J
authors.lastname: Futas
authors.forename: L
authors.initials: L
authors.lastname: Vychodilova
authors.forename: M
authors.initials: M
authors.lastname: Necesankova
mesh_terms: D000193:Actinomycetales Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006734:Horse Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D006736:Horses; D007109:Immunity / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D020395:Receptors, Interleukin-7 / Q000235:genetics*; D016929:Rhodococcus equi / Q000276:immunology* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014132:Trachea / Q000382:microbiology
chemical_list: D020395:Receptors, Interleukin-7