Control of cell attachment through polyDNA hybridization.
Cell-cell interactions play vital roles in embryo development and in homeostasis maintenance. Such interactions must be stringently controlled for cell-based tissue engineering and regenerative medicine therapies, and methods for studying and controlling cell-cell interactions are being developed using both biomedical and engineering approaches. In this study, we prepared amphiphilic PEG-lipid polymers that were attached to polyDNA with specific sequences. Incubation of cells with the polyDNA-PEG-lipid conjugate transferred some of the polyDNA to the cells' surfaces. Similarly, polyDNA-PEG-lipid conjugate using polyDNA with a complementary sequence was introduced to the surfaces of other cells or to a substrate surface. Cell-cell or cell-substrate attachments were subsequently mediated via hybridization between the two complementary polyDNAs and monitored using fluorescence microscopy.
Teramura
Yuji
Y
Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto, 606-8501, Japan.
Chen
Hao
H
Kawamoto
Takuo
T
Iwata
Hiroo
H
D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D008055:Lipids; D011108:Polymers; D011092:Polyethylene Glycols; D004247:DNA