title: Calorie restriction causes healthy life span extension in the filamentous fungus Podospora anserina.
abstract: Although most fungi appear to be immortal, some show systemic senescence within a distinct time frame. Podospora anserina for example shows an irreversible growth arrest within weeks of culturing associated with a destabilization of the mitochondrial genome. Here, we show that calorie restriction (CR), a regimen of under-nutrition without malnutrition, increases not only life span but also forestalls the aging-related decline in fertility. Similar to respiratory chain deficiencies the life span extension is associated with lower levels of intracellular H(2)O(2) measurements and a stabilization of the mitochondrial genome. Unlike respiratory chain deficiencies, CR cultures have a wild-type-like OXPHOS machinery similar to that of well-fed cultures as shown by native electrophoresis of mitochondrial protein complexes. Together, these data indicate that life span extension via CR is fundamentally different from that via respiratory chain mutations: Whereas the latter can be seen as a pathology, the former promotes healthy life span extension and may be an adaptive response.
authors.affiliation: Laboratory of Genetics, Department of Plant Sciences, Wageningen University, Arboretumlaan 4, 6703BD Wageningen, The Netherlands. anne.vandiepeningen@wur.nl
authors.forename: Anne D
authors.initials: AD
authors.lastname: van Diepeningen
authors.forename: Marc F P M
authors.initials: MF
authors.lastname: Maas
authors.forename: Daphne H E W
authors.initials: DH
authors.lastname: Huberts
authors.forename: Daniël J
authors.initials: DJ
authors.lastname: Goedbloed
authors.forename: Daniël J P
authors.initials: DJ
authors.lastname: Engelmoer
authors.forename: S Marijke
authors.initials: SM
authors.lastname: Slakhorst
authors.forename: A Bertha
authors.initials: AB
authors.lastname: Koopmanschap
authors.forename: Frank
authors.initials: F
authors.lastname: Krause
authors.forename: Norbert A
authors.initials: NA
authors.lastname: Dencher
authors.forename: Carole H
authors.initials: CH
authors.lastname: Sellem
authors.forename: Annie
authors.initials: A
authors.lastname: Sainsard-Chanet
authors.forename: Rolf F
authors.initials: RF
authors.lastname: Hoekstra
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Debets
mesh_terms: D000222:Adaptation, Physiological; D031204:Caloric Restriction; D004272:DNA, Mitochondrial / Q000378:metabolism; D005298:Fertility; D042822:Genomic Instability; D005947:Glucose / Q000172:deficiency; D006861:Hydrogen Peroxide / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D048468:Podospora / Q000502:physiology*; D013997:Time Factors
chemical_list: D004272:DNA, Mitochondrial; D006861:Hydrogen Peroxide; D005947:Glucose