Inhibition of enhanced green fluorescent protein expression by (dextran-hexamethylenediisocyanate)-g-polyethylenimine/siRNA complexes.
Small interfering RNA (siRNA), as a potential tool for gene therapy, requires suitable vehicles for stable complexation, protection, low cytotoxicity and high efficiency of gene knockdown. Here, a new polycation, (dextran-hexamethylenediisocyanate)-g-polyethylenimine ((Dex-HMDI)-g-PEI), was used as a siRNA vector to knock down the expression of Enhanced Green Fluorescent Protein (EGFP). It was found that the complexation of siRNA with the polycation can significantly knock down the expression of EGFP, but has no remarkable effect on the expression of luciferase.
Xiao
Wang
W
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Sun
Yun-Xia
YX
Cheng
Han
H
Zeng
Xuan
X
Zhang
Xian-Zheng
XZ
Zhuo
Ren-Xi
RX
D002460:Cell Line; D003485:Cyanates / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D017953:Isocyanates; D008156:Luciferases / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection
D003485:Cyanates; D003911:Dextrans; D017953:Isocyanates; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; C015262:1,6-hexamethylene diisocyanate; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D008156:Luciferases