title: Designed three-dimensional collagen scaffolds for skin tissue regeneration.
abstract: One of the challenges in tissue engineering is the development of a reproducible three-dimensional (3D) scaffold to support cell migration and infiltration. As a dermal substitute, 3D collagen scaffolds with precisely controlled pore structures were fabricated using an innovative cryogenic dispenser system. The scaffolds were composed of perpendicular, highly porous collagen strands in successive layers. The fabricated scaffolds were evaluated in an in vitro keratinocyte/fibroblast coculture test. Fibroblasts were well dispersed within the scaffold, and keratinocytes had completely migrated through the well-designed pore structure and differentiated on top of the scaffold surface. The differentiated keratinocytes generated a stratum corneum in the 3D dispensed scaffolds, similar to that in normal skin tissue.
authors.affiliation: Bio/Nanofluidics Laboratory, Department of Mechanical Engineering, Chosun University, Gwangju, Korea.
authors.forename: SeungHyun
authors.initials: S
authors.lastname: Ahn
authors.forename: Hyeon
authors.initials: H
authors.lastname: Yoon
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
authors.forename: YunYoung
authors.initials: Y
authors.lastname: Kim
authors.forename: SuHee
authors.initials: S
authors.lastname: Lee
authors.forename: Wook
authors.initials: W
authors.lastname: Chun
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012038:Regeneration; D012867:Skin / Q000166:cytology*
chemical_list: D003094:Collagen