title: [Efficacy comparison between transplanting microenvironmental induced and non-induced bone marrow mesenchymal stem cells in ischemic rat hearts].
abstract: OBJECTIVE
  To compare the efficacy of transplanting bone marrow mesenchymal stem cell (BMSC) or microenvironmental induced BMSC (iBMSC) into the ischemic myocardium of rats with myocardial infarction.
  
  
  METHODS
  iBMSC was defined as BMSC co-cultured with myocardial cells for 2 weeks. The stem cells or equal volume PBS were injected into ischemic border zone 1 wk after experimental infarction. Cardiac performance was evaluated at 1, 2, and 4 wk after cell transplantation by echocardiography and analyzed histologically at 4 wk after cell transplantations.
  
  
  RESULTS
  Compared with PBS group, both BMSC and iBMSC transplantations reduced infarct size. iBMSC enhanced the beneficial effects of BMSC on improving cardiac function (FS: 28.5% +/- 4.3% in PBS, 29.0% +/- 2.0% in BMSC and 45.1% +/- 3.1% in iBMSC group at 4 weeks post transplantation, iBMSC group vs. PBS group P < 0.05, iBMSC group vs. BMSC group P < 0.05). Immunofluorescence microscopy results revealed co-localization of SPIO-labeled transplanted cells with cardiac markers for cardiomyocytes, indicating regeneration of damaged myocardium.
  
  
  CONCLUSIONS
  Our data suggest that iBMSC implantation is more effective on improving cardiac function than BMSC implantation in this model. iBMSC might serve as a new promising therapeutic cell source for regenerating ischemic myocardium in patients with post-infarction heart failure.
authors.affiliation: Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Li
authors.forename: Yong-heng
authors.initials: YH
authors.lastname: Fu
authors.forename: Zai-yi
authors.initials: ZY
authors.lastname: Liu
authors.forename: Guang-feng
authors.initials: GF
authors.lastname: Zhang
authors.forename: Guang-fu
authors.initials: GF
authors.lastname: Dong
authors.forename: Qiu-xiong
authors.initials: QX
authors.lastname: Lin
authors.forename: Xi-yong
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002478:Cells, Cultured; D045164:Mesenchymal Stem Cell Transplantation; D009203:Myocardial Infarction / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019172:Transplantation Conditioning