title: Evaluation of apoptotic and anti-apoptotic genes on efficacy of DNA vaccine encoding glycoprotein B of Herpes Simplex Virus type 1.
abstract: Many approaches have so far been tried to enhance the immunogenicity of DNA vaccine. These include the use of various factors that induce apoptosis or anti-apoptosis effects when co-delivered with DNA vaccine. In the present study, the effects of pro-apoptotic Bax encoding plasmid (pBax) and anti-apoptotic Bcl-X(L) encoding plasmid (pBcl-xl), intradermally co-injected with glycoprotein B (gB) of Herpes Simplex Virus (HSV)-1 encoding plasmid (pgB) into the C57BL/6 mice were evaluated. Immune responses of the mice to the antigen were assessed by antibody assay, lymphoproliferative responses as well as cytokine and cytotoxic T-lymphocyte (CTL) assay. Analysis of the humoral and cellular responses showed that the mice immunized with pBax and pgB induced higher levels of antibody and Interleukin-4 as well as stronger lymphocyte proliferative responses and cytotoxic activity compared to those mice received pgB alone. pBcl-xl when intradermally co-injected with pgB showed no significant enhancement in immune responses comparing to pgB.
authors.affiliation: Dept of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Islamic Republic of Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Parsania
authors.forename: Taravat
authors.initials: T
authors.lastname: Bamdad
authors.forename: Zuhair Mohammad
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Maryam
authors.initials: M
authors.lastname: Kheirandish
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Pouriayevali
authors.forename: Rohollah Dorostkar
authors.initials: RD
authors.lastname: Sari
authors.forename: Abbas
authors.initials: A
authors.lastname: Jamali
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017209:Apoptosis / Q000235:genetics; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D015847:Interleukin-4 / Q000097:blood; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D016896:Treatment Outcome; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D051028:bcl-2-Associated X Protein / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D051020:bcl-X Protein / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C040495:glycoprotein B, Simplexvirus; D015847:Interleukin-4