Clickable fluorescent dyes for multimodal bioorthogonal imaging.
Bioorthogonal ligation with functionalized fluorescent dyes enables visualization of nuclei acids, proteins and metabolites in biological systems. Bright and modular azide- and alkyne-functionalized dyes are therefore needed to expand the fluorescence imaging capabilities of bioorthogonal ligation methods. We describe the concise synthesis of clickable fluorescent dyes based on 2-dicyanomethylene-3-cyano-2,5-dihydrofuran fluorophores and demonstrate their utility for multicolor imaging of azide- and alkyne-modified proteins as well as FRET studies.
Tsou
Lun K
LK
The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, USA.
Zhang
Mingzi M
MM
Hang
Howard C
HC
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D053858:Metabolic Networks and Pathways; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011506:Proteins