title: Prefabrication of tissue engineered bone grafts: an experimental study.
abstract: The purpose of this study was to determine whether angiogenesis could successfully be induced into bone tissue that was engineered by cultured adipose-derived stem cells with porous beta-tricalcium phosphate and whether its biologic properties could be maintained by flap prefabrication technique.Adipose-derived stem cells with porous beta-tricalcium phosphate were implanted into the superficial inferior epigastric artery flap of the Fisher rats. After prefabrication for 8 weeks, the prefabricated flaps were elevated and the pedicles were clamped for 4 hours. The samples were harvested after 2 weeks for analyses.Angiogenesis was significantly increased in the prefabricated groups (P < 0.05). There was no significant difference between the prefabricated and nonprefabricated groups in terms of the osteogenic capacity (P > 0.5).The promising results obtained with prefabrication in tissue engineered bone grafts encourage the clinical application of this technology. Thus, prefabrication may be a useful technique in any engineered bone tissue transfer.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Okuda
authors.forename: A Cagri
authors.initials: AC
authors.lastname: Uysal
authors.forename: Morikuni
authors.initials: M
authors.lastname: Tobita
authors.forename: Hiko
authors.initials: H
authors.lastname: Hyakusoku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000493:pharmacokinetics; D019074:Epigastric Arteries / Q000601:surgery; D016062:Porosity; D051381:Rats; D013234:Stem Cells / Q000378:metabolism; D013524:Surgical Flaps / Q000098:blood supply; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate