title: A novel function of the human CLS1 in phosphatidylglycerol synthesis and remodeling.
abstract: Phosphatidylglycerol (PG) is a precursor for the biosynthesis of cardiolipin and a signaling molecule required for various cellular functions. PG is subjected to remodeling subsequent to its de novo biosynthesis in mitochondria to incorporate appropriate acyl content for its biological functions and to prevent the harmful effect of lysophosphatidylglycerol (LPG) accumulation. Yet, a gene encoding a mitochondrial LPG acyltransferase has not been identified. In this report, we identified a novel function of the human cardiolipin synthase (hCLS1) in regulating PG remodeling. In addition to the reported cardiolipin synthase activity, the recombinant hCLS1 protein expressed in COS-7 cells and Sf-9 insect cells exhibited a strong acyl-CoA-dependent LPG acyltransferase activity, which was further confirmed by purified hCLS1 protein overexpressed in Sf-9 cells. The recombinant hCLS1 displayed an acyl selectivity profile in the order of in the order of C18:1>C18:2>C18:0>C16:0, which is similar to that of hCLS1 toward PGs in cardiolipin synthesis, suggesting that the PG remodeling by hCLS1 is an intrinsic property of the enzyme. In contrast, no significant acyltransferase activity was detected from the recombinant hCLS1 enzyme toward lysocardiolipin which shares a similar structure with LPG. In support of a key function of hCLS1 in PG remodeling, overexpression of hCLS1 in COS-7 cells significantly increased PG biosynthesis concurrent with elevated levels of cardiolipin without any significant effects on the biosynthesis of other phospholipids. These results demonstrate for the first time that hCLS1 catalyzes two consecutive steps in cardiolipin biosynthesis by acylating LPG to PG and then converting PG to cardiolipin.
authors.affiliation: Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, 500 University Drive, H166, Hershey, PA 17033, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Nie
authors.forename: Xinbao
authors.initials: X
authors.lastname: Hao
authors.forename: Daohong
authors.initials: D
authors.lastname: Chen
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Chang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008246:Lysophospholipids / Q000096:biosynthesis; D008565:Membrane Proteins / Q000502:physiology*; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D011994:Recombinant Proteins; D018411:Spodoptera; D017855:Transferases (Other Substituted Phosphate Groups) / Q000502:physiology*
chemical_list: D000214:Acyl Coenzyme A; D008246:Lysophospholipids; D008565:Membrane Proteins; D010715:Phosphatidylglycerols; D011994:Recombinant Proteins; C026223:lysophosphatidylglycerol; D000217:Acyltransferases; C496548:LPGAT1 protein, human; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase