title: Exploitation of biological wastes for the production of value-added hydrolases by Streptomyces sp. MSWC1 isolated from municipal solid waste compost.
abstract: Actinomycetes with the ability to degrade natural polysaccharides were isolated during a screening programme from soil, farmyard manure and municipal solid waste compost. One of the most potent isolates was identified as Streptomyces sp. MSWC1 using morphological and biochemical properties along with 16S rDNA partial sequence analysis. The highest enzyme production by Streptomyces was observed for the xylanase and chitinase activity on different carbon sources with an optimum of 12,100 IU ml(-1) and 110 IU ml(-1) at 3 days' culture on 1% of xylan and chitin, respectively. To meet the demand of industry, low-cost medium is required for the production of hydrolases by Streptomyces sp. Strain MSWC1 grown on manure, compost, and a natural carbon source was used to evaluate the re-utilisation of biological wastes for the production of value-added products. Despite the presence of a high amount of toxic heavy metals in the compost, Streptomyces produced interesting enzymes that have been biochemically characterized.
authors.affiliation: Laboratoire de Traitement et Recyclage des Eaux Usées, Centre des Recherches et des Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Mokni-Tlili
authors.forename: Imen
authors.initials: I
authors.lastname: Ben Abdelmalek
authors.forename: Naceur
authors.initials: N
authors.lastname: Jedidi
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.forename: Hassen
authors.initials: H
authors.lastname: Abdennaceur
authors.forename: Mohamed Nejib
authors.initials: MN
authors.lastname: Marzouki
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D002947:Cities; D006867:Hydrolases / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012037:Refuse Disposal / Q000379:methods*; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006867:Hydrolases