Label-free fluorescent real-time monitoring of adenylyl cyclase.
In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.
Rhee
Hyun-Woo
HW
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
Kim
Kyoung-Shim
KS
Han
Pyung-Lim
PL
Hong
Jong-In
JI
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D003199:Computer Systems; D005456:Fluorescent Dyes; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011725:Pyridines / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods
C526602:(1-((pyren-1-yl)propoxy)-3,5-bis(bis(2-pyridylmethyl)aminomethyl)benzene)zinc(II); D005456:Fluorescent Dyes; D011721:Pyrenes; D011725:Pyridines; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases