Production of a rat monoclonal antibody against Brg1.
Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.
Ohkawa
Yasuyuki
Y
SSP Stem Cell Unit, Department of Epigenetics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Harada
Akihito
A
Nakamura
Mako
M
Yoshimura
Saori
S
Tachibana
Taro
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000918:Antibody Specificity; D004265:DNA Helicases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats; D014157:Transcription Factors / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009687:Nuclear Proteins; D014157:Transcription Factors; C513194:Smarca4 protein, mouse; D004265:DNA Helicases