Evaluation of a caprine arthritis-encephalitis virus/maedi-visna virus indirect enzyme-linked immunosorbent assay in the serological diagnosis of ovine progressive pneumonia virus in U.S. sheep.
A caprine arthritis-encephalitis virus (CAEV)/maedi-visna virus (MVV) indirect enzyme-linked immunosorbent assay (iELISA) was validated with samples from U.S. sheep and by the use of radioimmunoprecipitation as the standard for comparison. The sensitivity and the specificity were 86.0% (+ or - 5.8%) and 95.9% (+ or - 2.9%), respectively. The iELISA format and phylogenetic differences based on the MVV gag sequence contribute to the reduced sensitivity.
Herrmann-Hoesing
Lynn M
LM
U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630, USA. lherrman@vetmed.wsu.edu
Broughton-Neiswanger
Liam E
LE
Gouine
Kimberly C
KC
White
Stephen N
SN
Mousel
Michele R
MR
Lewis
Gregory S
GS
Marshall
Katherine L
KL
Knowles
Donald P
DP
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016000:Cluster Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000175:diagnosis* / Q000821:virology; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012756:Sheep; D014481:United States; D014773:Virology / Q000379:methods*; D014790:Visna-maedi virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D012367:RNA, Viral