title: Circadian variations of prostaglandin E2 and F2 alpha release in the golden hamster retina.
abstract: Circadian variations of prostaglandin E2 and F2alpha release were examined in the golden hamster retina. Both parameters showed significant diurnal variations with maximal values at midnight. When hamsters were placed under constant darkness for 48 h, the differences in prostaglandin release between subjective mid-day and subjective midnight persisted. Western blot analysis showed that cyclooxygenase (COX)-1 levels were significantly higher at midnight than at mid-day, and at subjective midnight than at subjective mid-day, whereas no changes in COX-2 levels were observed among these time points. Immunohistochemical studies indicated the presence of COX-1 and COX-2 in the inner (but not outer) retina. Circadian variations of retinal prostaglandin release were also assessed in suprachiasmatic nuclei (SCN)-lesioned animals. Significant differences in retinal prostaglandin release between subjective mid-day and subjective midnight were observed in SCN-lesioned animals. These results indicate that hamster retinal prostaglandin release is regulated by a retinal circadian clock independent from the SCN. Thus, the present results suggest that the prostaglandin/COX-1 system could be a retinal clock output or part of the retinal clock mechanism.
authors.affiliation: Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de Bioquímica Humana, Facultad de Medicina/CEFyBO, Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina.
authors.forename: Nuria
authors.initials: N
authors.lastname: de Zavalía
authors.forename: Diego C
authors.initials: DC
authors.lastname: Fernandez
authors.forename: Pablo H
authors.initials: PH
authors.lastname: Sande
authors.forename: María I
authors.initials: MI
authors.lastname: Keller Sarmiento
authors.forename: Diego A
authors.initials: DA
authors.lastname: Golombek
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Rosenstein
authors.forename: Dafne M
authors.initials: DM
authors.lastname: Silberman
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D006224:Cricetinae; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003624:Darkness; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus / Q000033:anatomy & histology*; D009043:Motor Activity / Q000502:physiology; D017440:Photoperiod; D012160:Retina / Q000378:metabolism*; D013493:Suprachiasmatic Nucleus / Q000293:injuries / Q000502:physiology; D013997:Time Factors; D014316:Tritium / Q000378:metabolism
chemical_list: D014316:Tritium; D015237:Dinoprost; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone