The plant-derived natural compound Flavin 7 attenuates oxidative stress in cultured renal proximal tubule cells.
BACKGROUND
Cancer therapies and cancer progression can increase oxidative stress that might account for renal toxicity in cancer patients. Flavin 7 (F7) is a natural polyphenol-containing dietary supplement with potential antioxidant activity. Therefore, it might help to attenuate renal toxicity of chemotherapeutics.


METHODS
Cultured mouse renal proximal tubule cells were subjected to H(2)O(2)-mediated oxidative stress. Potential antioxidant effects of F7 were assessed by measuring the production of reactive oxygen species (ROS), mitochondrial depolarization and injury (lactate dehydrogenase release as well as trypan blue exclusion) in cells that were pretreated with F7 prior to treatment with H(2)O(2).


RESULTS
F7 pretreatment significantly attenuated H(2)O(2)-induced ROS production, mitochondrial depolarization and consequent injury in renal proximal tubule cells.


CONCLUSIONS
F7 supplementation might be beneficial for cancer patients in order to prevent renal toxicity of anticancer drug- or cancer progression-related oxidative stress.
Ember
Agoston
A
Department of Surgery, University of Pecs, Hungary.
Clark
Jeb S
JS
Varjas
Timea
T
Kiss
Istvan
I
Ember
Istvan
I
Baliga
Radhakrishna
R
Arany
Istvan
I
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001562:Benzimidazoles / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase