Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
Moser
S A
SA
University of Alabama at Birmingham, School of Medicine, Department of Pathology, Room P230, 619 19th St., South, Birmingham, AL 35249-7331, USA. moser@uab.edu
Mitchell
S C
SC
Ruby
J D
JD
Momeni
S
S
Osgood
R C
RC
Whiddon
J
J
Childers
N K
NK
D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D013290:Streptococcal Infections / Q000382:microbiology / Q000635:transmission; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification