[Simulation of corneal epithelial injuries by mechanical and corrosive damage : Influence of fetal bovine serum and dexpanthenol on epithelial regeneration in a cell culture model].
BACKGROUND
The present study describes simulation of corneal epithelial injury and its regeneration using an in-vitro model of immortalized human corneal epithelial cells (HCE-T) growing as monolayer cultures.


METHODS
The epithelial model was damaged using defined strengths by mechanical injury or partial damage using chemical detergents (SDS and acidified medium) and subsequently the epithelium was further cultivated using serum-containing and serum-free medium supplemented with varying concentrations of calcium pantothenat. After mechanical injury wound healing was evaluated using a photomicroscope over a period of up to 48 h whereas after chemical injury a cell viability assay was used to detect the course of ATP levels in the cell layers as an indicator for the metabolic activity.


RESULTS
Depending on the kind of injury pantothenat showed a regeneration enhancing effect in the concentration range from 0.001% to 0.01%. However, a concentration of 0.1% pantothenat appeared to be regeneration inhibiting. The combination of pantothenat and serum was more beneficial for wound healing than pantothenat alone, whereas serum partly levelled the effect of pantothenat.


CONCLUSIONS
The described model allowed simulation of corneal epithelial injury and its regeneration, whereby the influence of the serum content and the kind of injury could be determined.
Hahne
M
M
Institut für Pharmazeutische Technologie, Technische Universität Carolo-Wilhelmina zu Braunschweig, Deutschland.
Reichl
S
S
D000818:Animals; D002057:Burns, Chemical / Q000473:pathology*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D019573:Epithelium, Corneal / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D005126:Eye Burns / Q000473:pathology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008875:Middle Aged; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity; D013501:Surface-Active Agents / Q000633:toxicity
D013501:Surface-Active Agents; D010205:Pantothenic Acid; C007288:dexpanthenol; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate