Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves postthaw sperm membrane integrity.
In an attempt to evaluate the protective effect of a lipid-soluble antioxidant (butylated hydroxytoluene; BHT), semen from four dogs (Canis familiaris) was frozen in two different extenders (Uppsala or INRA-96 plus glycerol) with or without 1mM BHT. Sperm membrane integrity using flow cytometry and motility using a computerized system were evaluated in each experimental group. The Uppsala extender was superior in all aspects of sperm function. The percentage of sperm membranes was significantly higher in semen samples frozen in presence of BHT. Our results suggest that the Uppsala extender can be improved with the addition of BHT.
Neagu
V R
VR
Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
García
B Macías
BM
Sandoval
C Salazar
CS
Rodríguez
A Morillo
AM
Ferrusola
C Ortega
CO
Fernández
L González
LG
Tapia
J A
JA
Peña
F J
FJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D004285:Dogs; D005615:Freezing; D008297:Male; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D000975:Antioxidants; D003451:Cryoprotective Agents; D002084:Butylated Hydroxytoluene