title: Use of immunogold with silver enhancement.
abstract: Although gold particles are readily detectable by transmission electron microscopy, they can be difficult to visualize by bright-field light microscopy. However when the gold is silver-enhanced it is easy to see. During silver-enhancement, the colloidal gold serves as a nucleation site for the deposition of metallic silver. The enhancing solutions are physical developers that contain both silver ions and a reducing agent, buffered to an acid pH. The silver-enhancement method has also been used successfully to enlarge small-diameter gold particles for visualization by scanning electron microscopy. Silver-enhancement has been applied to a wide variety of tissues and antigens for both light and scanning electron microscopy.
authors.affiliation: Department of Cell and Molecular Biology, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Constance
authors.initials: C
authors.lastname: Oliver
mesh_terms: D000818:Animals; D017979:Gold Colloid / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007421:Intestine, Small / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008859:Microscopy, Polarization / Q000379:methods; D012834:Silver / Q000032:analysis*
chemical_list: D017979:Gold Colloid; D012834:Silver