A model for the formation and structure of casein micelles from subunits of variable composition.
Sedimentation coefficients were determined for association products in systems containing SH-kappa-casein, SH-kappa-casein-alpha(S1) or beta-caseins (1:1, by weight) and SH-kappa-casein-alpha(S1)-casein-beta-casein (2:1:1, by weight) under a variety of environmental conditions. As the concentration of NaCl is increased or, as the concentration of CaCl2 is increased at 0.05 M NaCl, the system containing SH-K-casein alone acts quite differently from those which also contain the Ca2+ -sensitive caseins. It is concluded that electrostatic as well as hydrophobic attractions are important between SH-kappa-casein monomers. Viscosity and sedimentation measurements combined indicate that nearly spherical polymers containing 25-30 monomers each and with radii of approx. 10 nm are formed in all systems. It is considered that these polymers are micelle subunits in which the non-polar portion of each monomer is oriented radially inward while the charged acidic peptides of the Ca2+ -sensitive caseins and the hydrophilic, carbohydrate-containing portion of kappa-casein are near the surface. Asymmetric distribution of kappa-casein in a micelle subunit results in hydrophilic and hydrophobic areas on the subunit surface. In this situation, aggregation through hydrophobic interactions forms a porous micelle. Micelle growth is limited by the eventual concentration, at the micelle surface, of subunits rich in kappa-casein. This model successfully accounts for the properties of equilibrium and nonequilibrium micelle systems, for the destabilization of the micelle by dephosphorylation of Ca2+ -sensitive caseins or by high concentrations of NaCl or CaCl2 and for the events associated with clot by rennin action.
Slattery
C W
CW
Department of Biochemistry, Loma Linda University School of Medicine, Loma Linda, California 92354, USA.
Evard
R
R
D000818:Animals; D002118:Calcium / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002364:Caseins / Q000737:chemistry*; D007477:Ions; D008823:Micelles; D008892:Milk; D010766:Phosphorylation; D016062:Porosity; D011506:Proteins / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature
D002364:Caseins; D007477:Ions; D008823:Micelles; D011506:Proteins; D012965:Sodium Chloride; D002122:Calcium Chloride; D002118:Calcium