Mouse mesenchymal stem cells can support human hematopoiesis both in vitro and in vivo: the crucial role of neural cell adhesion molecule.
BACKGROUND
We previously established a mesenchymal stem cell line (FMS/PA6-P) from the bone marrow adherent cells of fetal mice. The cell line expresses a higher level of neural cell adhesion molecule and shows greater hematopoiesis-supporting capacity in mice than other murine stromal cell lines.


METHODS
Since there is 94% homology between human and murine neural cell adhesion molecule, we examined whether FMS/PA6-P cells support human hematopoiesis and whether neural cell adhesion molecules expressed on FMS/PA6-P cells contribute greatly to the human hematopoiesis-supporting ability of the cell line.


RESULTS
When lineage-negative cord blood mononuclear cells were co-cultured on the FMS/PA6-P cells, a significantly greater hematopoietic stem cell-enriched population (CD34(+)CD38(-) cells) was obtained than in the culture without the FMS/PA6-P cells. Moreover, when lineage-negative cord blood mononuclear cells were cultured on FMS/PA6-P cells and transplanted into SCID mice, a significantly larger proportion of human CD45(+) cells and CD34(+)CD38(-) cells were detected in the bone marrow of SCID mice than in the bone marrow of SCID mice that had received lineage-negative cord blood mononuclear cells cultured without FMS/PA6-P cells. Furthermore, we found that direct cell-to-cell contact between the lineage-negative cord blood mononuclear cells and the FMS/PA6-P cells was essential for the maximum expansion of the mononuclear cells. The addition of anti-mouse neural cell adhesion molecule antibody to the culture significantly inhibited their contact and the proliferation of lineage-negative cord blood mononuclear cells.


CONCLUSIONS
These findings suggest that neural cell adhesion molecules expressed on FMS/PA6-P cells play a crucial role in the human hematopoiesis-supporting ability of the cell line.
Wang
Xiaoli
X
1st Dept of Pathology, Kansai Medical University, Moriguchi City, Osaka 570-8506, Japan.
Hisha
Hiroko
H
Mizokami
Tomomi
T
Cui
Wenhao
W
Cui
Yunze
Y
Shi
Aiping
A
Song
Changye
C
Okazaki
Satoshi
S
Li
Qing
Q
Feng
Wei
W
Kato
Junko
J
Ikehara
Susumu
S
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002460:Cell Line; D019070:Cell Lineage / Q000502:physiology; D018920:Coculture Techniques; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D019006:Neural Cell Adhesion Molecules / Q000502:physiology*; D013045:Species Specificity
D019006:Neural Cell Adhesion Molecules