Wrestling with native chemical ligation.
An improved method for the semisynthesis of a potassium channel involving native chemical ligation allows the introduction of short sequences containing non-canonical amino acids at any position within the polypeptide chain. The work enhances the technology available for a range of fundamental investigations of membrane proteins and for applications of membrane channels and pores in biotechnology.
Bayley
Hagan
H
Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom. hagan.bayley@chem.ox.ac.uk
Cheley
Stephen
S
Harrington
Leon
L
Syeda
Ruhma
R
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D001426:Bacterial Proteins / Q000138:chemical synthesis / Q000737:chemistry; D008958:Models, Molecular; D015221:Potassium Channels / Q000138:chemical synthesis* / Q000737:chemistry; D011487:Protein Conformation; D051381:Rats
D000596:Amino Acids; D001426:Bacterial Proteins; D015221:Potassium Channels; C107790:prokaryotic potassium channel