title: Amyloid formation: age-related mechanism in Creutzfeldt-Jakob disease?
abstract: Protein aggregation occurs in many age-related neurodegenerative diseases, where it can lead to deposits of naturally occurring proteins in the brain. In case of Creutzfeldt-Jakob disease (CJD), these deposits consist of prion protein (PrP). CJD has three etiologies: spontaneous, genetic, or caused by infection. A polymorphism within the PrP gene is associated with susceptibility of infection. The main event in prion diseases is the conversion of PrP from its naturally occurring isoform to its disease-associated isoform. Here, we present the adaption of a previously reported in vitro conversion system based on hamster recombinant PrP to analyze amyloid fibril formation of human recombinant PrP. We further compare the aggregation characteristics of the human PrP according to the polymorphism variants M129 and V129.
authors.affiliation: Institut fuer Physikalische Biologie, Heinrich-Heine Universitaet Duesseldorf, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Luers
authors.forename: G
authors.initials: G
authors.lastname: Panza
authors.forename: F
authors.initials: F
authors.lastname: Henke
authors.forename: T
authors.initials: T
authors.lastname: Agyenim
authors.forename: J
authors.initials: J
authors.lastname: Weiss
authors.forename: D
authors.initials: D
authors.lastname: Willbold
authors.forename: E
authors.initials: E
authors.lastname: Birkmann
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000682:Amyloid / Q000378:metabolism*; D052160:Benzothiazoles; D002942:Circular Dichroism; D003224:Congo Red / Q000494:pharmacology; D007562:Creutzfeldt-Jakob Syndrome / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D018620:PrPSc Proteins; D011994:Recombinant Proteins; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red