[Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2].
OBJECTIVE
Immortalized cell lines of spinocerebellar ataxia type 2 (SCA2) with Parkinson disease symptoms were established in order to provide experimental material for future study.


METHODS
The immortalized cell lines were constructed by using Epstein Barr virus and cyclosporine A. Microsatellite markers were detected to see whether there is any change between the cell lines and the original blood samples, and the genetic stability of the cell lines were evaluated.


RESULTS
Twenty-five immortalized cell lines were established successfully from the family and the microsatellite markers were unchanged.


CONCLUSIONS
The karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.
Lin
Ke-qin
KQ
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, 650118 P.R. China.
Sun
Hao
H
Zhang
Chang-jun
CJ
Tao
Yu-fen
YF
Yi
Wen
W
Huang
Xiao-qin
XQ
Shi
Li
L
Chu
Jia-you
JY
D000328:Adult; D044466:Asian People / Q000235:genetics; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D020754:Spinocerebellar Ataxias / Q000235:genetics*; D055815:Young Adult