title: Transplantation of hippocampal cell lines restore spatial learning in rats with ventral subicular lesions.
abstract: We have demonstrated in our previous studies that ventral subicular lesion induces neurodegeneration of the hippocampus and produces cognitive impairment in rats. In the present study, the efficacy of transplanted green fluorescent protein (GFP)-labeled hippocampal cell line (H3-GFP) cells in establishing functional recovery in ventral subicular lesioned rats has been evaluated. The survival of H3-GFP transplants and their ability to express trophic factors in vivo were also investigated. Adult male Wistar rats were subjected to selective lesioning of ventral subiculum and were transplanted with H3-GFP cells into the cornu ammonis 1 (CA1) hippocampus. The transplants settled mainly in the dentate gyrus and expressed neurotrophic factors, brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF). The ventral subicular lesioned (VSL) rats with H3-GFP transplants showed enhanced expression of BDNF in the hippocampus and performed well in eight-arm radial maze and Morris water maze tasks. The VSL rats without hippocampal transplants continued to show cognitive impairment in task learning. The present study demonstrated the H3-GFP transplants mediated recovery of cognitive functions in VSL rats. Our study supports the notion of graft meditated host regeneration and functional recovery through trophic support, although these mechanisms require further investigation.
authors.affiliation: Department of Neurophysiology, National Institute of Mental Health and Neuro Sciences (NIMHANS Deemed University), Bangalore, India.
authors.forename: J
authors.initials: J
authors.lastname: Rekha
authors.forename: Sridhara
authors.initials: S
authors.lastname: Chakravarthy
authors.forename: L R
authors.initials: LR
authors.lastname: Veena
authors.forename: Vani P
authors.initials: VP
authors.lastname: Kalai
authors.forename: Rupam
authors.initials: R
authors.lastname: Choudhury
authors.forename: Harsha N
authors.initials: HN
authors.lastname: Halahalli
authors.forename: Phalguni Anand
authors.initials: PA
authors.lastname: Alladi
authors.forename: Anandh
authors.initials: A
authors.lastname: Dhanushkodi
authors.forename: M
authors.initials: M
authors.lastname: Nirmala
authors.forename: Geetha M
authors.initials: GM
authors.lastname: Swamilingiah
authors.forename: Maulishree
authors.initials: M
authors.lastname: Agrahari
authors.forename: T R
authors.initials: TR
authors.lastname: Raju
authors.forename: M M
authors.initials: MM
authors.lastname: Panicker
authors.forename: Bindu M
authors.initials: BM
authors.lastname: Kutty
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002452:Cell Count; D002460:Cell Line; D017690:Cell Transplantation; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D018613:Microscopy, Confocal; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology; D013037:Spatial Behavior / Q000502:physiology; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins