title: Nuclear magnetic resonance quantification for monitoring heparosan K5 capsular polysaccharide production.
abstract: Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive (1)H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.
authors.affiliation: Rensselaer Polytechnic Institute, Biotechnology Center, 110 8th Street, Troy, NY 12180-3590, USA. wangz2@rpi.edu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Scott A
authors.initials: SA
authors.lastname: McCallum
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D053000:Analytic Sample Preparation Methods; D016667:Bacterial Capsules / Q000032:analysis*; D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D013997:Time Factors
chemical_list: C050673:capsular polysaccharide K5