Effect of sodium bicarbonate on Candida albicans adherence to thermally activated acrylic resin.
The purpose of this study was to evaluate the effect of 5% sodium bicarbonate on the adherence of Candida albicans to thermally activated acrylic resin. Fifty 4 mm(2) specimens of acrylic resin were obtained using a metallic matrix. The specimens received chemical polishing, were sterilized and then immersed in Sabouraud broth, inoculated with Candida albicans standardized suspension. After 24 hours of incubation at 37 degrees Celsius, the specimens were divided into four groups according to the substance used for disinfection (5% sodium bicarbonate, 0.12% digluconate chlorhexidine, vinegar and Corega Tabs). A control group was included, in which distilled water was used. The adhered microorganisms were dispersed, diluted and plated onto culture media to determine the number of colony-forming units (cfu/mL). The results were analyzed through the Mann-Whitney statistical test at the 5% level of significance. Only 0.12% digluconate chlorhexidine and 5% sodium bicarbonate presented a statistically significant difference (p = 0.0010 and p = 0.0156, respectively) compared to the control group, decreasing the number of cfu/mL. However, when the different disinfecting solutions were compared with each other, only 0.12% digluconate chlorhexidine presented a statistically significant difference in the reduction of cfu/mL. It was concluded that although 0.12% digluconate chlorhexidine was more effective in the reduction of Candida albicans adherence values to thermally activated acrylic resin, 5% sodium bicarbonate also proved to be a viable alternative.
Sousa
Fernando Augusto Cervantes Garcia de
FA
Department of Bioscience and Oral Diagnosis, Dentistry of São José dos Campos, São Paulo State University, São José dos Campos, SP, Brazil. facgs@uol.com.br
Paradella
Thaís Cachuté
TC
Koga-Ito
Cristiane Yumi
CY
Jorge
Antonio Olavo Cardoso
AO
D000180:Acrylic Resins / Q000737:chemistry*; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000494:pharmacology; D003820:Denture Cleansers / Q000494:pharmacology; D003824:Denture, Complete / Q000382:microbiology; D006358:Hot Temperature; D008422:Materials Testing; D017693:Sodium Bicarbonate / Q000737:chemistry*; D018709:Statistics, Nonparametric; D013282:Stomatitis, Denture / Q000628:therapy; D013499:Surface Properties; D013997:Time Factors
D000180:Acrylic Resins; D019551:Dental Disinfectants; D003820:Denture Cleansers; D017693:Sodium Bicarbonate