Constituents of Corydalis heterocarpa and their anti-proliferative effects on human cancer cells.
Two new coumarins, 1 and 2, along with four known coumarins (3-6) have been isolated from Corydalis heterocarpa. On the basis of spectroscopic and chemical methods, compounds 1 and 2 were elucidated as (2'S,7'S)-O-2-methylbutanoyl-columbianetin and (2'S)-columbianetin-3'-sulfate, respectively. The anti-proliferative activity against human cancer cells of compounds 1-6 isolated from C. heterocarpa was evaluated using a MTT assay and by mRNA expression of several factors related to apoptosis. Among them, compound 2 exerted the more potent anti-proliferative activity compared with the other compounds treated. The potent inhibitory effect of compound 2 was produced by induction of apoptosis through activating Bax, p53 and p21 expressions.
Kim
You Ah
YA
Division of Marine Environment and Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.
Kong
Chang-Suk
CS
Yea
Sung Su
SS
Seo
Youngwan
Y
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005562:Formazans / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism
C548752:(2'S)-columbianetin-3'-sulfate; C548753:(2'S,7'S)-O-2-methylbutanoyl-columbianetin; D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; D005562:Formazans; D010936:Plant Extracts; D013778:Tetrazolium Salts; C079782:MTT formazan