title: Mercury-associated DNA hypomethylation in polar bear brains via the LUminometric Methylation Assay: a sensitive method to study epigenetics in wildlife.
abstract: In this paper we describe a novel approach that may shed light on the genomic DNA methylation of organisms with non-resolved genomes. The LUminometric Methylation Assay (LUMA) is permissive for genomic DNA methylation studies of any genome as it relies on the use of methyl-sensitive and -insensitive restriction enzymes followed by polymerase extension via Pyrosequencing technology. Here, LUMA was used to characterize genomic DNA methylation in the lower brain stem region from 47 polar bears subsistence hunted in central East Greenland between 1999 and 2001. In these samples, average genomic DNA methylation was 57.9% +/- 6.69 (SD; range was 42.0 to 72.4%). When genomic DNA methylation was related to brain mercury (Hg) exposure levels, an inverse association was seen between these two variables for the entire study population (P for trend = 0.17). After dichotomizing animals by gender and controlling for age, a negative trend was seen amongst male animals (P for trend = 0.07) but no associations were found in female bears. Such sexually dimorphic responses have been found in other toxicological studies. Our results show that genomic DNA methylation can be quantitatively studied in a highly reproducible manner in tissue samples from a wild organism with a non-resolved genome. As such, LUMA holds great promise as a novel method to explore consequential questions across the ecological sciences that may require an epigenetic understanding.
authors.affiliation: Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: J Richard
authors.initials: JR
authors.lastname: Pilsner
authors.forename: Alicia L
authors.initials: AL
authors.lastname: Lazarus
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Nam
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Letcher
authors.forename: Christian
authors.initials: C
authors.lastname: Sonne
authors.forename: Rune
authors.initials: R
authors.lastname: Dietz
authors.forename: Niladri
authors.initials: N
authors.lastname: Basu
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects*; D019175:DNA Methylation; D004785:Environmental Pollutants; D044127:Epigenesis, Genetic; D005260:Female; D006116:Greenland; D008297:Male; D008628:Mercury / Q000494:pharmacology*; D001503:Ursidae / Q000235:genetics*
chemical_list: D004785:Environmental Pollutants; D008628:Mercury