Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer.
Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1-5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K(d)) of 4.95 +/- 0.31 and 7.05 +/- 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 microg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.
Kim
Mina
M
Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea.
Um
Hyun-Ju
HJ
Bang
Sunbaek
S
Lee
Sang-Hee
SH
Oh
Suk-Jung
SJ
Han
Ji-Hye
JH
Kim
Kyoung-Woong
KW
Min
Jiho
J
Kim
Yang-Hoon
YH
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001151:Arsenic / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D045483:Rivers / Q000737:chemistry; D052156:SELEX Aptamer Technique; D020349:Surface Plasmon Resonance / Q000379:methods; D014744:Vietnam; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000295:instrumentation / Q000379:methods*; D014881:Water Supply / Q000032:analysis*
D052157:Aptamers, Nucleotide; D014874:Water Pollutants, Chemical; D001151:Arsenic