Correlation between histological type and immunohistochemical profile of prostate cancer and gleason scale gradation.
OBJECTIVE
To evaluate the characteristics of prostate cancer (PC) morphogenesis, taking into consideration the role of proliferation and apoptosis in tumor cells.


METHODS
p53, p16INK4a, Bcl-2 and Ki-67 proteins expression was analyzed by immunohistochemistry in paraffin embedded sections of biopsy specimens from PC patients. The level of tissue immunoreactivity was evaluated by semi-quantitative method with estimation of 100% colored cells content over 1000 cells in one specimen. Patients were divided into three groups in accordance to Gleason scale gradation: group 1 - with Gleason scale < 5 (n = 13); group 2 - with Gleason scale > 5 and < 8 (n = 8); group 3 - with the highest Gleason scale > 8 (n = 6).


RESULTS
Upon histological examination of prostate biopsy specimens, it was found that in the first group in 6 out of 13 (46%) cases small acinic cell PC developed on the background of chronic prostatitis with PIA (proliferative inflammatory atrophy) locus, frequently in combination with prostatic intraepithelial neoplasia (PI) locus. Hyperchromic epithelial cells in PIA locus were characterized by nuclear expression of p53 and Ki67 proteins, and cytoplasmic expression of Bcl-2. The precancerous foci in the PIN and PIA in the biopsy specimens of the second group of PC patients were found in 2 out of 8 (25%) cases of large and small acinic cell adenocarcinoma observations. The expression level of p53, p16INK4a, Bcl-2 proteins and especially Ki67 protein adequately increased in tumors of group 2 in comparison with group 1. Group 3 comprised of patients with Gleason scale > 8, predominantly solid structures or scirrhus of PC, which were characterized by the highest nuclear expression of p53, p16INK4a and Ki-67, and also by overexpression of cytoplasmic Bcl-2.


CONCLUSIONS
Obtained results showed the direct correlation between patients' Gleason scale, and the expression level of p53, p16INK4a, Bcl-2 proteins and, particularly, Ki67 marker of proliferating cells in PC tumor cells.
Iemelynova
A A
AA
Institute of Urology, Ukrainian Academy of Medical Sciences, Kiev 04053, Ukraine.
Grygorenko
V M
VM
Cheremuha
S V
SV
Romanenko
A M
AM
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D017209:Apoptosis / Q000502:physiology; D014408:Biomarkers, Tumor / Q000032:analysis*; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53