Functionalization of cellulose acetate fibers with engineered cutinases.
In the present work, we describe for the first time the specific role of cutinase on surface modification of cellulose acetate fibers. Cutinase exhibits acetyl esterase activity on diacetate and triacetate of 0.010 U and 0.007 U, respectively. An increase on the hydroxyl groups at the fiber surface of 25% for diacetate and 317% for triacetate, after a 24 h treatment, is estimated by an indirect assay. Aiming at further improvement of cutinase affinity toward cellulose acetate, chimeric cutinases are genetically engineered by fusing the 3'-end coding sequence with a bacterial or a fungal carbohydrate-binding module and varying the linker DNA sequence. A comparative analysis of these genetic constructions is presented showing that, the superficial regeneration of cellulose hydrophilicity and reactivity on highly substituted cellulose acetates is achieved by chimeric cutinases.
Matamá
Teresa
T
Textile Engineering Dept., University of Minho, Campus Azurém, 4800-058 Guimarães, Portugal.
Araújo
Rita
R
Gübitz
Georg M
GM
Casal
Margarida
M
Cavaco-Paulo
Artur
A
D019342:Acetic Acid / Q000378:metabolism; D000880:Anthraquinones; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005670:Fusarium / Q000201:enzymology / Q000235:genetics; D008856:Microscopy, Fluorescence; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D000880:Anthraquinones; D011994:Recombinant Proteins; C005062:acetylcellulose; D002482:Cellulose; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; C024758:Remazol Brilliant Blue R; D019342:Acetic Acid