title: Generation of transgenic rats by microinjection of short DNA fragments.
abstract: Here we describe an efficient technique to generate transgenic rats by microinjection of short DNA fragments. We have focused on optimal conditions for superovulation of prepubescent females Sprague-Dawley (CD) strains to have good quality embryos, pseudopregnant females, zygotes preparation, optimal conditions for microinjection and embryo transfer into foster mothers.
authors.affiliation: INSERM, UMR643, Nantes, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.forename: Séverine
authors.initials: S
authors.lastname: Remy
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D008297:Male; D008845:Microinjections / Q000379:methods*; D010062:Ovulation Induction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic / Q000235:genetics*; D019076:Transgenes; D015053:Zygote / Q000166:cytology
chemical_list: D004247:DNA