Inflammatory alterations in excluded colon in rats: a comparison with chemically induced colitis.
Diversion colitis occurs commonly in the large bowel remnant after diversion of the fecal stream. Several experimental models of colitis have been described, but none examine the inflammatory alterations that can occur in experimentally defunctioned colons. This characterization could be useful in understanding pathophysiological aspects of diversion colitis, and in developing future therapeutic strategies. Thus, we evaluated the temporal inflammatory alterations in the defunctioned colon of rats by analyzing the histological results, infiltrating neutrophils, pro-inflammatory markers such as cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS), and DNA damage in isolated colonocytes. We compared the obtained data with those from hapten-induced colitis. The experimental diversion of the colon fecal stream induces diversion colitis characterized by an early inflammatory process with increased neutrophil infiltrate, and COX-2 and iNOS expression that resembles, in some aspects, the inflammatory characteristics of chemically induced colitis. After acute inflammation resolution, there was an increase in COX-2 and iNOS expression and the presence of lymphoid follicular hyperplasia and ulcerations, suggesting that diversion colitis can be experimentally established and useful for studying different pathophysiological aspects of this condition.
Longatti
Thamara Sigrist
TS
Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Bragança Paulista, São Paulo, Brazil.
Acedo
Simone Coghetto
SC
de Oliveira
Caroline Candida
CC
Miranda
Daniel Duarte da Conceição
DD
Priolli
Denise Gonçalves
DG
Ribeiro
Marcelo Lima
ML
Gambero
Alessandra
A
Martinez
Carlos Augusto Real
CA
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2