A novel approach to the structural analysis of partially decorated actin based filaments.
We describe a novel set of single particle based procedures for the structural analysis of electron microscope images of muscle thin filaments and other partially decorated actin based filaments. The thin filament comprises actin and the regulatory proteins tropomyosin and troponin in a 7:1:1M ratio. Prior to our work, structure analysis from electron microscope images of the thin filament has largely involved either helical averaging defined by the underlying actin helix or the use of single particle analysis but using a starting model as a reference structure. Our single particle based approach yields an accurate structure for the complete thin filament by avoiding the loss of information from troponin and tropomyosin associated with helical averaging and also removing the potential reference bias associated with the use of a starting model. The approach is more widely applicable to sub-stoichiometric complexes of F-actin and actin-binding proteins.
Paul
Danielle M
DM
Institute of Cancer Research, Chester Beatty Laboratories, 237, Fulham Road, London SW3 6JB, UK.
Squire
John M
JM
Morris
Edward P
EP
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism; D008854:Microscopy, Electron; D009132:Muscles / Q000737:chemistry / Q000648:ultrastructure*; D011487:Protein Conformation; D014336:Troponin / Q000737:chemistry / Q000378:metabolism
D000199:Actins; D008840:Microfilament Proteins; D014336:Troponin