Antitumor and antiplatelet activity of alkaloids from veratrum dahuricum.
Ten steroidal alkaloids - cyclopamine, veratramine, jervine, 3, 15-diangyloylgermine, 3-angyloylzygadenine, 3-veratroyl zygadenine, 15-veratroylgermine, germine, veratrosine and pseudojervine - from Veratrum dahuricum, together with the ethanol extract and total alkaloids, were evaluated for their antitumor and antiplatelet activities. Cyclopamine, veratramine and germine significantly inhibited the hedgehog pathway in NIH/3T3 cells. Cyclopamine exerted a potent inhibitory effect against the growth of PANC-1 tumors in mice, with inhibition rates of 40.64%, 44.37%, 46.77% at doses of 5.0, 15.0 and 50.0 mg kg-1, respectively. Veratroylgermine was found to produce the strongest inhibition against the platelet aggregation induced by arachidonic acid, with inhibition rate of 92.0% at 100 microM.
Tang
Jian
J
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China.
Li
Hui-Liang
HL
Shen
Yun-Heng
YH
Jin
Hui-Zi
HZ
Yan
Shi-Kai
SK
Liu
Xiao-Hua
XH
Zeng
Hua-Wu
HW
Liu
Run-Hui
RH
Tan
Ye-Xiong
YX
Zhang
Wei-Dong
WD
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D014703:Veratrum / Q000737:chemistry*; D014704:Veratrum Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D014704:Veratrum Alkaloids