Potato virus X as a novel platform for potential biomedical applications.
We demonstrate that nanoparticles formed from the rod-shaped plant virus Potato virus X (PVX) can serve as a novel platform for biomedical applications. Bioconjugation protocols including amine modification and "click" chemistry allowed the efficient functionalization of PVX with biotins, dyes, and PEGs. Fluorescent-labeled and PEGylated PVX particles revealed that different fluorescent labels have a profound effect on PVX-cell interactions. Applying bioconjugation chemistries to PVX opens the door for chemical functionalization with targeting and therapeutic molecules.
Steinmetz
Nicole F
NF
Department of Cell Biology, Center for Integrative Molecular Biosciences, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Mertens
Marianne E
ME
Taurog
Rebecca E
RE
Johnson
John E
JE
Commandeur
Ulrich
U
Fischer
Rainer
R
Manchester
Marianne
M
D001709:Biotechnology / Q000379:methods; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D011092:Polyethylene Glycols / Q000737:chemistry; D017863:Potexvirus / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012984:Software; D014466:Ultraviolet Rays
D005456:Fluorescent Dyes; D012367:RNA, Viral; D011092:Polyethylene Glycols; D001710:Biotin