title: The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel.
abstract: The stress-activated kinase Hog1p mediates arsenic tolerance by decreasing arsenite influx through the aquaglyceroporin Fps1p in Saccharomyces cerevisiae. Unexpectedly, we found that overexpression of FPS1 increased arsenite tolerance suggesting a physiological role of Fps1p in arsenic detoxification. Consistently, during arsenite treatment transcription of FPS1 gene was strongly upregulated, while Fps1p was not degraded and remained localized to the plasma membrane. Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux.
authors.affiliation: Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Maciaszczyk-Dziubinska
authors.forename: Iwona
authors.initials: I
authors.lastname: Migdal
authors.forename: Magdalena
authors.initials: M
authors.lastname: Migocka
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bocer
authors.forename: Robert
authors.initials: R
authors.lastname: Wysocki
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D051397:Aquaglyceroporins / Q000235:genetics / Q000378:metabolism; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites / Q000378:metabolism*; D001692:Biological Transport; D015153:Blotting, Western; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C109281:ACR3 protein, S cerevisiae; D018528:ATP-Binding Cassette Transporters; D051397:Aquaglyceroporins; D018053:Arsenites; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C089179:YCF1 protein, S cerevisiae; D049452:Green Fluorescent Proteins; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; C015001:arsenite; D001151:Arsenic