Genomic inventory and transcriptional analysis of Medicago truncatula transporters.
Transporters move hydrophilic substrates across hydrophobic biological membranes and play key roles in plant nutrition, metabolism, and signaling and, consequently, in plant growth, development, and responses to the environment. To initiate and support systematic characterization of transporters in the model legume Medicago truncatula, we identified 3,830 transporters and classified 2,673 of these into 113 families and 146 subfamilies. Analysis of gene expression data for 2,611 of these transporters identified 129 that are expressed in an organ-specific manner, including 50 that are nodule specific and 36 specific to mycorrhizal roots. Further analysis uncovered 196 transporters that are induced at least 5-fold during nodule development and 44 in roots during arbuscular mycorrhizal symbiosis. Among the nodule- and mycorrhiza-induced transporter genes are many candidates for known transport activities in these beneficial symbioses. The data presented here are a unique resource for the selection and functional characterization of legume transporters.
Benedito
Vagner A
VA
Genetics and Developmental Biology Program, Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, USA.
Li
Haiquan
H
Dai
Xinbin
X
Wandrey
Maren
M
He
Ji
J
Kaundal
Rakesh
R
Torres-Jerez
Ivone
I
Gomez
S Karen
SK
Harrison
Maria J
MJ
Tang
Yuhong
Y
Zhao
Patrick X
PX
Udvardi
Michael K
MK
D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D046913:Medicago truncatula / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics*; D055170:Plant Root Nodulation; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013559:Symbiosis
D026901:Membrane Transport Proteins; D010940:Plant Proteins