The evolution of real-time PCR machines to real-time PCR chips.
Development of Micro-Electro-Mechanical Systems (MEMS) technology has recently allowed the migration of real-time polymerase chain reaction (PCR) machines to lab-on-a-chip systems. The miniaturization of biological instruments has been studied extensively, with several prototypes constructed and tested. In this study, the lab-on-a-chip system is evaluated; its DNA quantification is estimated by theorems, and the specifications of proposed chip prototypes are compared with the original machines. The analysis results suggest five hypotheses. Using experiments and the data collected from published papers, these hypotheses were either verified or rejected, and the advantages and shortcomings of real-time PCR chips were identified. The proven advantages of the lab-on-a-chip system are its compact size, low sample volume to nano-liter, and short analysis time-less than 10s to complete one PCR cycle and 370 s for completing the whole quantification process. However, the detection limits, quantification uncertainties, and melting analysis ability of the chip prototypes are at best comparable to, and perhaps worse than, those of commercial instruments. Real-time PCR chips are not perfectly accurate diagnostic tools for a laboratory but they have advantages over traditional techniques for point-of-care testing.
Lee
Dasheng
D
Department of Energy and Refrigerating Air-Conditioning Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung Hsiao E. Rd., Taipei 106, Taiwan, ROC. f11167@ntut.edu.tw
Chen
Pei-Jer
PJ
Lee
Gwo-Bin
GB
D003199:Computer Systems; D019544:Equipment Failure Analysis; D005544:Forecasting; D046228:Microarray Analysis / Q000295:instrumentation* / Q000639:trends*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000639:trends*; D013673:Technology Assessment, Biomedical