Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches.
HepG-2 cells are widely used as a cell model to investigate hepatocellular carcinomas and the effect of anticancer drugs such as doxorubicin, an effective antineoplastic agent, which has broad antitumoral activity against many solid and hematological malignancies. To investigate the effect of doxorubicin on the protein pattern, we used complementary proteomic workflows including 2-D gel-based and gel-free methods. The analysis of crude HepG2 cell extracts by 2-D DIGE provided data on 1835 protein spots which was then complemented by MS-centered analysis of stable isotope labeling by amino acids in cell culture-labeled cells. The monitoring of more than 1300 distinct proteins, including proteins of the membrane fraction provides the most comprehensive overview on the proteome of the widely used model cell line HepG2. Of the proteins monitored in total, 155 displayed doxorubicin-induced changes in abundance. Functional analysis revealed major influences of doxorubicin on proteins involved in protein synthesis, DNA damage control, electron transport/mitochondrial function, and tumor growth. The strongest decrease in level was found for proteins involved in DNA replication and protein synthesis, whereas proteins with a function in DNA damage control and oxidative stress management displayed increased levels following treatment with doxorubicin compared with control cells. Furthermore, the doxorubicin-associated increase in levels of multiple forms of keratins 8, 18, and 19 and other structural proteins revealed an influence on the cytoskeleton network.
Hammer
Elke
E
Interfaculty Institute for Genetics and Functional Genomics, Ernst-Moritz-Arndt Universität, Greifswald, Germany. hammer@uni-greifswald.de
Bien
Sandra
S
Salazar
Manuela Gesell
MG
Steil
Leif
L
Scharf
Christian
C
Hildebrandt
Petra
P
Schroeder
Henry W S
HW
Kroemer
Heyo K
HK
Völker
Uwe
U
Ritter
Christoph A
CA
D002470:Cell Survival / Q000187:drug effects; D002853:Chromatography, Liquid; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D056945:Hep G2 Cells; D006801:Humans; D014176:Protein Biosynthesis / Q000187:drug effects; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D020543:Proteome; D004317:Doxorubicin; D004247:DNA