The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U1, U2, U5 and U4/U6 and pre-mRNAs. The mutations in the N-terminus, which prevent splicing to occur, include in vitro and in vivo identified phosphorylation sites of Prp4 kinase. These sites are highly conserved in the human ortholog U5-102 K. The results presented here demonstrate that structural integrity of the N-terminus is required to mediate a splicing event, but is not necessary for the assembly of spliceosomes.
Lützelberger
Martin
M
Institute of Genetics, University of Braunschweig TU, Spielmannstr. 7, 38106 Braunschweig, Germany.
Bottner
Claudia A
CA
Schwelnus
Wiebke
W
Zock-Emmenthal
Susanne
S
Razanau
Aleh
A
Käufer
Norbert F
NF
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D000072260:RNA Splicing Factors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D017381:Spliceosomes / Q000378:metabolism*
C109024:PRP1 protein, S pombe; D000072260:RNA Splicing Factors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D029702:Schizosaccharomyces pombe Proteins; D017346:Protein Serine-Threonine Kinases; C489981:prp4 protein, S pombe