title: Analysing the expression of protein kinase C eta in human hepatocellular carcinoma.
abstract: OBJECTIVE
  Protein kinase Cs (PKCs) play important roles in cell proliferation, differentiation, apoptosis, migration and tumorigenesis. In this report, we investigated the expression of PKCeta in human hepatocellular carcinoma (HCC) tissues and explored its role in the development of HCC.
  
  
  METHODS
  We used real-time quantitative RT-PCR, mutation analysis, and immunohistochemical staining to analyse the expression of PKCeta in 50 pairs of human hepatocellular carcinoma (HCC) tissues.
  
  
  RESULTS
  Expression of PKCeta was down-regulated in 82% of HCC tissues and the reduction of PKCeta was associated with poorer long-term survival of HCC patients.
  
  
  CONCLUSIONS
  Reduced expression of PKCeta may represent a molecular lesion in the development of more aggressive disease of HCC.
authors.affiliation: Departments of Laboratory Medicine and Clinical Research, Kaohsiung Medical University Hospital, Institute of Clinical Research, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Hsiu-Chin
authors.initials: HC
authors.lastname: Lu
authors.forename: Fen-Pi
authors.initials: FP
authors.lastname: Chou
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Ya-Sian
authors.initials: YS
authors.lastname: Chang
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Hsu
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007527:Isoenzymes; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm; D007527:Isoenzymes; C081975:protein kinase C eta; D011493:Protein Kinase C