title: Levels of oxidative damage and lipid peroxidation in thyroid neoplasia.
abstract: BACKGROUND
  This study assessed the presence of oxidative damage and lipid peroxidation in thyroid neoplasia.
  
  
  METHODS
  Using tissue microarrays and immunohistochemistry, we assessed levels of DNA damage (8-oxo-dG) and lipid peroxidation (4-HNE) in 71 follicular thyroid adenoma (FTA), 45 papillary thyroid carcinoma (PTC), and 17 follicular thyroid carcinoma (FTC) and matched normal thyroid tissue.
  
  
  RESULTS
  Cytoplasmic 8-oxo-dG and 4-HNE expression was significantly higher in FTA, FTC, and PTC tissue compared to matched normal tissue (all p values < .001). Similarly, elevated nuclear levels of 8-oxo-dG were seen in all in FTA, FTC, and PTC tissue compared to matched normal (p values < .07, < .001, < .001, respectively). In contrast, a higher level of 4-HNE expression was detected in normal thyroid tissue compared with matched tumor tissue (p < .001 for all groups). Comparing all 3 groups, 4-HNE levels were higher than 8-oxo-dG levels (p < .001 for all groups) except that cytoplasmic levels of 8-oxo-dG were higher than 4-HNE in all (p < .001). These results were independent of proliferation status.
  
  
  CONCLUSIONS
  High levels of DNA damage and lipid peroxidation in benign and malignant thyroid neoplasia indicates this damage is an early event that may influence disease progression.
authors.affiliation: Department of Otolaryngology, Head & Neck Surgery, UCD School of Medicine & Medical Science, St Vincent's University Hospital, Elm Park, Dublin.
authors.forename: Orla
authors.initials: O
authors.lastname: Young
authors.forename: Tom
authors.initials: T
authors.lastname: Crotty
authors.forename: Rohana
authors.initials: R
authors.lastname: O'Connell
authors.forename: Jacintha
authors.initials: J
authors.lastname: O'Sullivan
authors.forename: Aongus J
authors.initials: AJ
authors.lastname: Curran
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000236:Adenoma / Q000503:physiopathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000447:Aldehydes / Q000378:metabolism; D000911:Antibodies, Monoclonal; D003593:Cytoplasm / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D040081:Protein Array Analysis; D013964:Thyroid Neoplasms / Q000503:physiopathology*; D055815:Young Adult
chemical_list: D000447:Aldehydes; D000911:Antibodies, Monoclonal; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal