title: Basic oxygen furnace slag as a treatment material for pathogens: contribution of inactivation and attachment in virus attenuation.
abstract: Basic oxygen furnace (BOF) slag media were studied as a potential treatment material in on-site sanitation systems. Batch and column studies were conducted to evaluate attenuation of the bacteriophage PR772 and 0.190 microm diameter microspheres by BOF media, and to delineate the relative contributions of two principle processes of virus attenuation: inactivation and attachment. In the batch studies, conducted at 4 degrees C, substantial inactivation of PR772 did not occur in the pH 7.6 and 9.5 suspensions. At pH 11.4, bimodal inactivation of PR772 was observed, at an initial rate of 2.1 log C/C(0) day(-1) for the first two days, followed by a much slower rate of 0.124 log C/C(0) day(-1) over the following 10 days. Two column studies were conducted at 4 degrees C at a flow rate of 1 pore volume day(-1) using two slag sources (Stelco, Ontario; Tubarão, Brazil) combined with sand and pea gravel. In both column experiments, the effluent microsphere concentration approached input concentrations over time (reductions of 0.1-0.2 log C/C(0)), suggesting attachment processes for microspheres were negligible. Removal of PR772 virus was more pronounced both during the early stages of the experiments, but also after longer transport times (0.5-1.0 log C/C(0)). PR772 reduction appeared to be primarily as a result of virus inactivation in response to the elevated pH conditions generated by the BOF mixture (10.6-11.4). On-site sanitation systems using BOF media should be designed to maintain sufficient contact time between the BOF media and the wastewater to allow sufficient residence time of pathogens at elevated pH conditions.
authors.affiliation: Department of Earth and Environmental Sciences, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1. jesses.timson@amec.com
authors.forename: Jesse
authors.initials: J
authors.lastname: Stimson
authors.forename: Gi-Tak
authors.initials: GT
authors.lastname: Chae
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Ptacek
authors.forename: Monica B
authors.initials: MB
authors.lastname: Emelko
authors.forename: Maria M
authors.initials: MM
authors.lastname: Mesquita
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Hirata
authors.forename: David W
authors.initials: DW
authors.lastname: Blowes
mesh_terms: D001435:Bacteriophages / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D052918:Environmental Restoration and Remediation; D005374:Filtration / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D016062:Porosity; D012499:Sanitation / Q000379:methods*; D012722:Sewage / Q000737:chemistry / Q000821:virology*; D053585:Virus Attachment; D038301:Virus Inactivation; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D012722:Sewage