Detection of serum beta(2)-GPI-Lp(a) complexes in patients with systemic lupus erythematosus.
BACKGROUND
Circulating beta(2)-glycoprotein-I-oxidized low-density lipoprotein (beta(2)-GPI-ox-LDL) complexes have been found in patients with systemic lupus erythematosus (SLE) and other autoimmune diseases as a contributor to the development of autoimmune-mediated atherosclerosis. In vitro study showed that beta(2)-GPI also bound with high affinity to atherogenic lipoprotein (a) [Lp(a)] which shares structural similarity to LDL. We examined the existence and clinical significance of serum complexes of beta(2)-GPI with Lp(a) in SLE patients.


METHODS
A "sandwich" ELISA was developed for measuring serum concentrations of beta(2)-GPI-Lp(a) complexes, using rabbit anti-human beta(2)-GPI antibody as capturing antibody, and quantitating with antibody against apo(a). Forty-seven SLE patients and 42 healthy controls were studied.


RESULTS
Both Lp(a) (400+/-213 mg/l vs. 181+/-70 mg/l) and ox-Lp(a) (27.07+/-22.30 mg/l vs. 8.20+/-4.55 mg/l) concentrations were higher in SLE patients than in controls (P<0.0001). beta(2)-GPI-Lp(a) complexes were detectable in both controls and SLE. The complexes levels in SLE were higher than in controls (0.96+/-0.41 U/ml vs. 0.59+/-0.20 U/ml, P<0.0001) and was positively correlated with ox-Lp(a) (P<0.001).


CONCLUSIONS
We report the existence of beta(2)-GPI-Lp(a) complexes in both controls and SLE patients. The complexes levels increase in SLE.
Zhang
Chunni
C
Department of Biochemistry, Jinling Hospital, Clinical School of Medical College, Nanjing University, 210002, Nanjing, PR China.
Li
Ke
K
Shi
Beilei
B
Wang
Xiangdong
X
Liu
Xiaozhuan
X
Qin
Weisong
W
Han
Aizhong
A
Wang
Junjun
J
D000328:Adult; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood*; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I / Q000097:blood* / Q000302:isolation & purification
D017270:Lipoprotein(a); D053482:beta 2-Glycoprotein I