Nematodetoxic aurovertin-type metabolites from a root-knot nematode parasitic fungus Pochonia chlamydosporia.
Chemical investigation of one fungal strain P. chlamydosporia YMF 1.00613 isolated from root knots of tobacco infected by Meloidogyne incognita led to the isolation and identification of four aurovertin-type metabolites, which include a new compound, aurovertin I (A1), and three known metabolites, aurovertins E, F and D (A2-A4). Their structures were established by spectroscopic studies such as 1D- and 2D-NMR and MS analysis. Aurovertin I (A1) is the first natural product with an aurovertin skeleton with one less carbon. Compounds A3 and A4 showed the toxicity to the worms of the free-living nematode Panagrellus redivevus with the LC(50) values 88.6 and 41.7 microg/mL at 48 h, respectively. All four aurovertins did not show obvious inhibitory effects on egg hatch of root knot nematode Meloidogyne incognita. The results suggested that the aurovertin-type metabolites produced by P. chlamydosporia might be one of the pathogenic factors involved in the suppression of nematodes.
Niu
Xue-Mei
XM
Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
Wang
Yan-Li
YL
Chu
Yan-Sheng
YS
Xue
Hua-Xi
HX
Li
Nan
N
Wei
Lu-Xia
LX
Mo
Ming-He
MH
Zhang
Ke-Qin
KQ
D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D001313:Aurovertins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D006999:Hypocreales / Q000737:chemistry / Q000378:metabolism*; D009348:Nematoda / Q000187:drug effects / Q000502:physiology; D010935:Plant Diseases / Q000469:parasitology; D014026:Nicotiana / Q000469:parasitology; D014431:Tylenchoidea / Q000187:drug effects / Q000382:microbiology*
D000969:Antinematodal Agents; D001313:Aurovertins