[Comparative analysis of measles genome between vaccine strain and wild-type strain in Zhejiang province of China].
OBJECTIVE
To explore the distinction between wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level.


METHODS
After sequencing of measles wild-stain MVi/Zhejiang. CHN/7.05/4, the distinction between the wild-type strain and the vaccine strain was analysed by MEGA 3.1 software at genome level, and the antigen variation was studied by means of combining the epidemiological data.


RESULTS
There were 822 nucleotide differences (5.17%) and 161 amino acid differences between these two strains, including three glycosylation sites variation found. Meanwhile, the antigen ratio between wild-type strain and vaccine strain was found to be 5.66.


CONCLUSIONS
There should be certain differences between the contemporary wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level, and the protective effects of measles vaccine should be studied further.
Xu
Chang-ping
CP
Center for Disease Control and Prevention of Zhejiang Province, Key Laboratory of Emergence Detection for Public Health of Zhejiang Province, Hangzhou 310051, China.
Yan
Ju-ying
JY
Feng
Yan
Y
Mao
Hai-yan
HY
Lu
Yi-yu
YY
D000940:Antigenic Variation; D002681:China / Q000453:epidemiology; D055028:Comparative Genomic Hybridization; D016679:Genome, Viral; D006801:Humans; D008457:Measles / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D008458:Measles Vaccine / Q000235:genetics*; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D020539:Sequence Analysis, Protein; D017423:Sequence Analysis, RNA
D008458:Measles Vaccine