Preparation of myosin A from body wall musculature of Ascaris lumbricoides.
Myosin A (Ascaris myosin A) was prepared from the body wall musculature of Ascaris lumbricoides var. suis showing a single peak in analytical ultracentrifuge pattern, and its sedimentation coefficient was about 6 S which was close to the values reported for other myosins. The ATPase activities determined in various media were 3-4 times lower than those of rabbit skeletal myosin A. Nevertheless, the maximum level of the superprecipitation of actomyosin was 4 times higher for Ascaris myosin A than for rabbit skeletal myosin A in the presence of 0.01 mM ATP. It appears that Ascaris myosin A is capable of contracting the actomyosin system in a lower concentration of ATP than that for the usual actomyosin system.
Yamaguchi
M
M
Department of Biochemistry and Nutrition, School of Physical Education, Juntendo University, Bunkyo-ku Hongo, Tokyo, Japan.
Nakamura
T
T
Oya
H
H
Sekine
T
T
D000205:Actomyosin / Q000737:chemistry*; D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D017164:Ascaris lumbricoides / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry; D018482:Muscle, Skeletal / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D024507:Nonmuscle Myosin Type IIA / Q000737:chemistry* / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature; D013997:Time Factors; D014461:Ultracentrifugation / Q000379:methods*
D000255:Adenosine Triphosphate; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D024507:Nonmuscle Myosin Type IIA; D002118:Calcium