Synthesis and biological evaluation of new potential inhibitors of N-acylethanolamine hydrolyzing acid amidase.
N-Acylethanolamines, including N-palmitoyl-ethanolamine (PEA), are hydrolyzed to the corresponding fatty acids and ethanolamine by fatty acid amide hydrolase (FAAH). Recently, N-acylethanolamine-hydrolyzing acid amidase (NAAA) was identified as being able to specifically hydrolyze PEA. In order to find selective and effective inhibitors of this enzyme, we synthesized and screened several amides, retroamides, esters, retroesters and carbamates of palmitic acid (1-21) and esters with C15 and C17 alkyl chains (22-27). Cyclopentylhexadecanoate (13) exhibited the highest inhibitory activity on NAAA (IC(50)=10.0 microM), without inhibiting FAAH up to 50 microM. Compound 13 may become a useful template to design new NAAA inhibitors.
Saturnino
Carmela
C
Department of Pharmaceutical Sciences, University of Salerno, Via Ponte don Melillo 84084, Fisciano, SA, Italy.
Petrosino
Stefania
S
Ligresti
Alessia
A
Palladino
Chiara
C
De Martino
Giovanni
G
Bisogno
Tiziana
T
Di Marzo
Vincenzo
V
D000577:Amides; D000581:Amidohydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D063388:Endocannabinoids; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D004983:Ethanolamines; D006801:Humans; D006868:Hydrolysis; D010169:Palmitic Acids / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000577:Amides; D063388:Endocannabinoids; D004791:Enzyme Inhibitors; D004983:Ethanolamines; D010169:Palmitic Acids; C005958:palmidrol; D000581:Amidohydrolases; C523108:NAAA protein, human