Shape-coded silica nanotubes for multiplexed bioassay: rapid and reliable magnetic decoding protocols.
OBJECTIVE
The recent development of 1D barcode arrays has proved their capabilities to be applicable to highly multiplexed bioassays. This article introduces two magnetic decoding protocols for suspension arrays of shape-coded silica nanotubes to process multiplexed assays rapidly and easily, which will benefit the minimization and automation of the arrays.


METHODS
In the first protocol, the magnetic nanocrystals are incorporated into the inner voids of barcoded silica nanotubes in order to give the nanotubes magnetic properties. The second protocol is performed by trapping the barcoded silica nanotubes onto streptavidin-modified magnetic beads.


RESULTS
The rapid and easy decoding process was demonstrated by applying the above two protocols to multiplexed assays, resulting in high selectivity. Furthermore, the magnetic bead-trapped barcode nanotubes provided a great opportunity to exclude the use of dye molecules in multiplexed assays by using barcode nanotubes as signals.


CONCLUSIONS
The rapid and easy manipulation of encoded carriers using magnetic properties could be used to develop promising suspension arrays for portable bioassays.
He
Bo
B
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
Kim
Sung Kyoung
SK
Son
Sang Jun
SJ
Lee
Sang Bok
SB
D000818:Animals; D001331:Automation; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013997:Time Factors
D002272:Carcinoembryonic Antigen; D011506:Proteins; D012822:Silicon Dioxide