title: Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition.
abstract: A strained aza-dibenzocyclooctyne was prepared via a high-yielding synthetic route. Copper-free, strain-promoted click reaction with azides showed excellent kinetics, and a functionalised aza-cyclooctyne was applied in fast and efficient PEGylation of enzymes.
authors.affiliation: Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, NL-6525 AJ Nijmegen, The Netherlands.
authors.forename: Marjoke F
authors.initials: MF
authors.lastname: Debets
authors.forename: Sander S
authors.initials: SS
authors.lastname: van Berkel
authors.forename: Sanne
authors.initials: S
authors.lastname: Schoffelen
authors.forename: Floris P J T
authors.initials: FP
authors.lastname: Rutjes
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
authors.forename: Floris L
authors.initials: FL
authors.lastname: van Delft
mesh_terms: D001372:Aza Compounds / Q000737:chemistry*; D064026:Calbindins; D003300:Copper / Q000737:chemistry; D003516:Cycloparaffins / Q000737:chemistry*; D007700:Kinetics; D011092:Polyethylene Glycols / Q000737:chemistry*; D064030:S100 Calcium Binding Protein G / Q000737:chemistry*
chemical_list: D001372:Aza Compounds; D064026:Calbindins; D003516:Cycloparaffins; D064030:S100 Calcium Binding Protein G; D011092:Polyethylene Glycols; D003300:Copper