title: Separation and preconcentration of trace level of lead in one drop of blood sample by using graphite furnace atomic absorption spectrometry.
abstract: Drop-to-drop solvent microextraction (DDSME) assisted with ultrasonication is applied for the determination of lead in one drop (30 microL) of blood sample by using graphite furnace atomic absorption spectrometry (GF-AAS). The optimum extraction efficiency of lead was observed for 10 min extraction time at pH 5.0 with 2 microL of organic solvent that containing 0.5 M of Cyanex-302. The optimized methodology exhibited good linearity in the range of 0.3-30.0 ng mL(-1) lead with relative standard deviations (RSD) from 2.5 to 4.4%. The method is found to be simple and rapid for the analysis of lead in micro amount of blood sample with the limit of detection (LOD) of 0.08 ng mL(-1). The application of the proposed method has been successfully tested for the determination of lead in blood samples. The results showed that under the optimized experimental conditions, the method showed good sensitivity and recovery %, as well as advantages such as linearity, simplicity, low cost and high feasibility.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville pike, Building 29A, 2B20, Bethesda, MD 20892, United States. shrikam@rediffmail.com
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Shrivas
authors.forename: Devesh Kumar
authors.initials: DK
authors.lastname: Patel
mesh_terms: D005240:Feasibility Studies; D006801:Humans; D007854:Lead / Q000097:blood* / Q000302:isolation & purification; D057230:Limit of Detection; D008722:Methods; D015203:Reproducibility of Results; D012997:Solvents; D013054:Spectrophotometry, Atomic / Q000379:methods* / Q000592:standards
chemical_list: D012997:Solvents; D007854:Lead