Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems.
Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalization of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies.
García-Alonso
Javier
J
Department of Biological Sciences, University of Hull, Hull HU6 7RX, UK. j.garcia-alonso@nhm.ac.uk
Fakhrullin
Rawil F
RF
Paunov
Vesselin N
VN
D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism*; D018189:Immunomagnetic Separation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008904:Miniaturization; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D049452:Green Fluorescent Proteins