title: In vivo and in vitro investigations of fungal keratitis caused by Colletotrichum gloeosporioides.
abstract: OBJECTIVE
  To report a case of fungal keratitis caused by Colletotrichum gloeosporioides, which is a rare pathogen in humans.
  
  
  METHODS
  An 80-year-old woman developed fungal keratitis after having sustained a traumatic injury during field work. The patient was initially examined by slit-lamp biomicroscopy and the Heidelberg Retina Tomograph II-Rostock Cornea Module (HRT II-RCM). Corneal scrapings were collected and submitted for laboratory investigations.
  
  
  RESULTS
  Many septate, hyphae-like interlocking and branching white lines were observed in the area of the infiltrate by HRT II-RCM. A tentative diagnosis of fungal keratitis was made, and the patient was treated with systemic and topical voriconazole and pimaricin ophthalmic ointment. The infectious focus resolved within 2 weeks, and there were no signs of a recurrence after 3 months of treatment with the antifungal agents. The culture of the corneal scraping grew C. gloeosporioides.
  
  
  CONCLUSIONS
  HRT II-RCM was useful in detecting filamentous fungi in the cornea. The treatment with voriconazole and pimaricin was effective in the treatment of C. gloeosporioides keratitis.
authors.affiliation: Department of Ophthalmology, Ehime University School of Medicine, Shitsukawa, Toon-City, Ehime, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Mitani
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiraishi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Uno
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000008:administration & dosage / Q000627:therapeutic use; D020231:Colletotrichum / Q000302:isolation & purification*; D004359:Drug Therapy, Combination; D015821:Eye Infections, Fungal / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D006801:Humans; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology; D010866:Natamycin / Q000008:administration & dosage / Q000627:therapeutic use; D011743:Pyrimidines / Q000008:administration & dosage / Q000627:therapeutic use; D016896:Treatment Outcome; D014230:Triazoles / Q000008:administration & dosage / Q000627:therapeutic use; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D010866:Natamycin; D065819:Voriconazole