IgG is transported across the mouse yolk sac independently of FcgammaRIIb.
While generally accepted that FcRn of the human syncytiotrophoblast and the mouse yolk sac endoderm is the major IgG transporter, the finding of a different Fc receptor FcgammaRIIb (RIIb) in the human placental endothelium has suggested the existence of an additional IgG transporter. Testing our hypothesis in mouse, we found that while RIIb is expressed in the yolk sac vasculature, IgG concentrations in fetuses of wild-type mice (RIIb(+/+)) and mice with a null mutation in the gene encoding RIIb (RIIb(-/-) mice) are not different, and we thus reject our hypothesis that yolk sac RIIb transports IgG in utero in the mouse. However, the capillary bed in the mouse yolk sac is structurally more complex than in human placenta, consisting of three types of cells: an RIIb-negative endothelium, a unique RIIb-bearing cell that also expresses 2 out of 4 macrophage markers but not endothelial cell or pericyte markers, and pericytes. As in the human placenta the b2 isoform of RIIb predominates in the mouse yolk sac. Remarkably only a single capillary channel rather than 2 channels with a loop is found in each yolk sac villus, which, along with intracapillary erythrocytes, suggests that blood flow is peristaltic, mediated by pericytes. It is not clear whether RIIb in the human placental villus might mediate an IgG transport function in light of the mouse yolk sac equivalent failing to do so.
Mohanty
Sudhasri
S
Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA. anderson.48@osu.edu
Kim
Jonghan
J
Ganesan
Latha P
LP
Phillips
Gary S
GS
Hua
Keding
K
Jarjoura
David
D
Hayton
William L
WL
Robinson
John M
JM
Anderson
Clark L
CL
D000818:Animals; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D021041:Placental Circulation; D011247:Pregnancy; D020033:Protein Isoforms / Q000276:immunology / Q000378:metabolism*; D021381:Protein Transport / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism*; D013045:Species Specificity; D014168:Transferrin / Q000096:biosynthesis; D015017:Yolk Sac / Q000378:metabolism*
C499172:Fcgr2b protein, mouse; D007074:Immunoglobulin G; D020033:Protein Isoforms; D017452:Receptors, IgG; D014168:Transferrin