title: Deparaffinization and processing of pathologic material.
abstract: Methods for preparation of whole nuclei from paraffin embedded tissue are given. The combination of cell sorting of isolated nuclei combined with the polymerase chain reaction can be used to investigate other cellular parameters such as nuclear proteins and proliferation factors.
authors.affiliation: Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cunningham
mesh_terms: D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods*; D010336:Pathology / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA