title: CE-ESI-TOF/MS for human growth hormone analysis.
abstract: CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE-ESI-TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
authors.forename: Aline
authors.initials: A
authors.lastname: Staub
authors.forename: Sylvain
authors.initials: S
authors.lastname: Giraud
authors.forename: Martial
authors.initials: M
authors.lastname: Saugy
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey
authors.forename: Julie
authors.initials: J
authors.lastname: Schappler
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D019382:Human Growth Hormone / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000097:Acetonitriles; D019382:Human Growth Hormone; C032159:acetonitrile