title: Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS.
abstract: In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R(ct)) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.
authors.affiliation: Hanoi University of Technology, Hanoi 8404, Viet Nam.
authors.forename: Thi Ngoc Lien
authors.initials: TN
authors.lastname: Truong
authors.forename: Dai Lam
authors.initials: DL
authors.lastname: Tran
authors.forename: Thi Hong An
authors.initials: TH
authors.lastname: Vu
authors.forename: Vinh Hoang
authors.initials: VH
authors.lastname: Tran
authors.forename: Tuan Quang
authors.initials: TQ
authors.lastname: Duong
authors.forename: Quang Khieu
authors.initials: QK
authors.lastname: Dinh
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Lee
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D017796:Caulimovirus / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017097:Electric Impedance; D004563:Electrochemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D030781:Organisms, Genetically Modified; D011108:Polymers / Q000737:chemistry*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011758:Pyrroles / Q000737:chemistry*; D011791:Quartz; D013025:Glycine max / Q000235:genetics; D013057:Spectrum Analysis; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D037742:Nanotubes, Carbon; D009838:Oligodeoxyribonucleotides; D011108:Polymers; D011758:Pyrroles; D011791:Quartz; C067635:polypyrrole; D004247:DNA