title: Confocal microscopy evidence of prion protein fragment hPrP[173-195] internalization in rat B104 neuroblastoma cell line.
abstract: The cytotoxicity of hPrP[173-195] prion peptide against a neuroblastoma cell model was found independent of its tendency to aggregate over time. Cytosolic and nuclear inclusions of peptide were highlighted by confocal microscopy, suggesting a role as a transcription factor in activating signal transduction pathways involved in cell toxicity.
authors.affiliation: Lab. of Adaptive Physiology, Department of Biological and Environmental Science and Technology, University of Lecce, prov.le Lecce-Monteroni, Lecce, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Urso
authors.forename: Raffaele
authors.initials: R
authors.lastname: Acierno
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Lionetto
authors.forename: Antonia
authors.initials: A
authors.lastname: Rizzello
authors.forename: Andrea
authors.initials: A
authors.lastname: Papa
authors.forename: Trifone
authors.initials: T
authors.lastname: Schettino
authors.forename: Michele
authors.initials: M
authors.lastname: Maffia
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019793:Fluorescein; D018613:Microscopy, Confocal / Q000379:methods*; D009447:Neuroblastoma / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D017096:Prion Diseases; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D051381:Rats; D012235:Rhodamines; D015398:Signal Transduction; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D018621:PrPC Proteins; D012235:Rhodamines; C005358:tetramethylrhodamine; D013752:Tetracycline; D019793:Fluorescein