Lipoic acid blocks seizures induced by pilocarpine via increases in delta-aminolevulinic dehydratase and Na+, K+-ATPase activity in rat brain.
In the present study we investigated the effects of lipoic acid (LA) on delta-aminolevulinic dehydratase (delta-ALA-D) and Na(+), K(+)-ATPase activities in rat brain after seizures induction by pilocarpine. Wistar rats were treated with 0.9% saline (i.p., control group), lipoic acid (10mg/kg, i.p., LA group), pilocarpine (400mg/kg, i.p., pilocarpine group), or the combination of LA (10mg/kg, i.p.) with pilocarpine (400mg/kg, i.p.), 30 min before administration of LA (LA plus pilocarpine group). After the treatments all groups were observed for 1h. The enzyme activities (delta-ALA-D and Na(+), K(+)-ATPase) were measured using spectrophotometric methods, and the results were compared with that obtained from saline and pilocarpine-treated animals. Neuroprotective effects of LA against seizures were evaluated based on those enzyme activities. The pilocarpine group showed a reduction in delta-ALA-D and Na(+), K(+)-ATPase activities after seizures. In turn, LA plus pilocarpine abolished the appearance of seizures and reversed the decreased in delta-ALA-D and Na(+), K(+)-ATPase activities produced by seizures, when compared to the pilocarpine seizing group. The results from the present study demonstrate that preadministration of LA abolished seizure episodes induced by pilocarpine in rat, probably by increasing delta-ALA-D and Na(+), K(+)-ATPase activities in rat brain during seizures.
Santos
Itala Mônica de Sales
IM
Laboratory of Experimental Research in Biological Sciences of Federal University of Piauí, Piauí, Brazil.
Tomé
Adriana da Rocha
Ada R
Feitosa
Chistiane Mendes
CM
de Souza
Geane Felix
GF
Feng
Dejiang
D
de Freitas
Rivelilson Mendes
RM
Jordán
Joaquín
J
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010862:Pilocarpine / Q000009:adverse effects*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D008063:Thioctic Acid / Q000008:administration & dosage*
D010862:Pilocarpine; D008063:Thioctic Acid; D000623:Porphobilinogen Synthase; D000254:Sodium-Potassium-Exchanging ATPase