title: Terbutryn toxicity to Danio rerio: effects of subchronic exposure on fish growth.
abstract: OBJECTIVE
  The aim of this study was to investigate effects of subchronic exposure to sublethal levels of terbutryn on growth and on histopathological changes in Danio rerio.
  
  
  METHODS
  The acute toxicity tests were performed on the juvenile stage of Danio rerio according to OECD No. 203 Fish, Acute Toxicity Test. The juvenile growth tests were performed on D. rerio according to the OECD method No. 215. Fish at the age of 20 days were exposed to the terbutryn environmental concentration commonly detected in the Czech rivers (0.02 microg L-1) and the range of sublethal concentrations of terbutryn (0.06, 0.2, 0.6 and 2 mg L-1) for 28 days.
  
  
  RESULTS
  The 96hLC50 terbutryn mean value for the juvenile stage of D. rerio was 5.71 +/- 0.46 mg L-1. A significant decrease (p < 0.01) in the fish growth caused by terbutryn was observed in the concentration of 0.6 mg L-1. The value of NOEC was 0.2 mg L-1 and LOEC was 0.6 mg L-1 of terbutryn. We found the damage to tubular system of kidneys in the concentration of 0.6 mg L-1 of terbutryn.
  
  
  CONCLUSIONS
  The environmental concentration of terbutryn in the Czech rivers did not have any effects on growth and on histopathological changes in D. rerio and this concentration is lower than determined LOEC and NOEC values of terbutryn.
authors.affiliation: Department of Veterinary Public Health and Toxicology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. lplhalova@vfu.cz
authors.forename: Lucie
authors.initials: L
authors.lastname: Plhalova
authors.forename: Stanislava
authors.initials: S
authors.lastname: Macova
authors.forename: Ivana
authors.initials: I
authors.lastname: Haluzova
authors.forename: Andrea
authors.initials: A
authors.lastname: Slaninova
authors.forename: Petra
authors.initials: P
authors.lastname: Dolezelova
authors.forename: Petr
authors.initials: P
authors.lastname: Marsalek
authors.forename: Vladimira
authors.initials: V
authors.lastname: Pistekova
authors.forename: Iveta
authors.initials: I
authors.lastname: Bedanova
authors.forename: Eva
authors.initials: E
authors.lastname: Voslarova
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D049628:Body Size / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects*; D018153:Czech Republic; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D045483:Rivers; D018675:Toxicity Tests; D014227:Triazines / Q000008:administration & dosage / Q000633:toxicity*; D014873:Water Pollutants / Q000008:administration & dosage / Q000633:toxicity; D015027:Zebrafish / Q000254:growth & development*
chemical_list: D006540:Herbicides; D014227:Triazines; D014873:Water Pollutants; C010347:terbutryne