title: Development and validation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of four anthocyanins in human plasma after black currant anthocyanins ingestion.
abstract: Black currant anthocyanins consist of delphinidin-3-glucoside (1), delphinidin-3-rutinoside (2), cyanidin-3-glucoside (3), and cyanidin-3-rutinoside (4). A liquid chromatography tandem mass spectrometry method for simultaneous determination of four anthocyanins in human plasma was developed and validated. Samples were prepared using solid phase extraction, followed by chromatographic separation with a reverse phase C(18) column with gradient elution using mobile phases containing water, acetonitrile, and formic acid. The quantification of four anthocyanins was determined by multiple reaction monitoring using electrospray ionization. The method showed good selectivity, sensitivity (limits of quantification for four anthocyanins were 0.2 nmol/L), linearity (0.2-20 nmol/L; r > 0.999), intra- and interday precision, accuracy (<14%), and recovery (62.5-85.7%). Analyte stability was investigated in detail. This method was successfully applied to the determination of delphinidin-3-glucoside (1), delphinidin-3-rutinoside (2), cyanidin-3-glucoside (3), and cyanidin-3-rutinoside (4) concentrations in human plasma after ingestion of a single dose of black currant anthocyanins (87.9 micromol (58.8 mg) total anthocyanins).
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd, Sakado-shi, Saitama 350-0289, Japan. yuko_nakamura@meiji.co.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Morifuji
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iida
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D000872:Anthocyanins / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004435:Eating; D005260:Female; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*; D031965:Ribes / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts