title: Influence of the hot-fill water-spray-cooling process after continuous pasteurization on the number of decimal reductions and on Alicyclobacillus acidoterrestris CRA 7152 growth in orange juice stored at 35 degrees C.
abstract: In this study, the influence of the hot-fill water-spray-cooling process after continuous pasteurization on the number of decimal reductions (gamma) and growth parameters (lag time; lambda, ratio N(f)/N(o); kappa, maximum growth rate; mu) of Alicyclobacillus acidoterrestris CRA 7152 in orange juice stored at 35 degrees C were investigated. Two different inoculum levels of A. acidoterrestris CRA 7152 (10(2) and 10(3) spores/mL) in orange juice (11(0)Brix, pH 3.7) and a Microthermics UHT-HTST pilot plant were used to simulate industrial conditions. Results have shown that regardless of the inoculum level (10(2) or 10(3) spores/mL), the pasteurization processes were unable to cause even 1 gamma. Predictive modeling using the Baranyi model showed that only kappa and time to reach 10(4)spores/mL (t10(4) - time to juice spoilage) were affected by the spore inoculum used (p<0.05). It has been concluded that A. acidoterrestris was able to survive the hot-fill process and to grow and spoil orange juice in 5-6 days when the final storage temperature was 35 degrees C.
authors.affiliation: Department of Food Science, Faculty of Food Engineering, State University of Campinas, Rua Monteiro Lobato, 80, Caixa Postal: 6121. 13083-862, Campinas, SP, Brazil.
authors.forename: Ana Cláudia N F
authors.initials: AC
authors.lastname: Spinelli
authors.forename: Anderson S
authors.initials: AS
authors.lastname: Sant'Ana
authors.forename: Cristiana P
authors.initials: CP
authors.lastname: Pacheco-Sanchez
authors.forename: Pilar R
authors.initials: PR
authors.lastname: Massaguer
mesh_terms: D056450:Alicyclobacillus / Q000254:growth & development* / Q000528:radiation effects*; D001628:Beverages / Q000382:microbiology*; D032084:Citrus sinensis / Q000382:microbiology*; D003080:Cold Temperature; D015169:Colony Count, Microbial; D005511:Food Handling / Q000379:methods*; D005519:Food Preservation; D006358:Hot Temperature; D050296:Microbial Viability; D013997:Time Factors