The androgen cytosol receptor in the ventral prostate of the rat. Methodological problems.
The phenomena of aggregation and stability of the 8-S androgen receptor in the cytosol fraction from rat ventral prostate have been studied. When glycerol was present both in the cytosol fraction and in the sucrose density gradients, a well-defined and easily reproducible 8-S peak was obtained after incubation with tritiated 5alpha-dihydrotestosterone. Without glycerol in the in vitro system the receptor appeared as polydispersed aggregates. Addition of glycerol to the steroid-free cytosol made it possible to store the cytosol at -70 degrees C or -196 degrees C for at least I week without losing 8-S receptor activity. The counting efficiency was studied in different liquid scintillators. A simple toluene scintillation liquid gave the highest counting efficiency.
HØisaeter
P A
PA
Institute of Anatomy and Department of General Surgery, University of Bergen, Bergen, Norway.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D003600:Cytosol / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000737:chemistry / Q000378:metabolism*; D012588:Scintillation Counting; D014050:Toluene / Q000737:chemistry
D000728:Androgens; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D014050:Toluene; D005990:Glycerol