[Effects of protein kinase CK2alpha on the proliferation and invasion of human nasopharyngeal carcinoma].
OBJECTIVE
To investigate the effect of protein kinase CK2alpha expression on the proliferation and invasion of human nasopharyngeal carcinoma and related mechanism.


METHODS
RNA interference (RNAi) was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells. The biological behavior of treated cells were analyzed by MTT and in-vitro invasion assays. Cell proliferation cycle was examined by flow cytometry and the phosphorylation status of Akt protein was examined by Western blotting analysis.


RESULTS
CK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the proliferative and invasive abilities of 5-8F cells. Flow cytometry analysis showed that the percentage of cells increased in G(0)/G(1) phase but decreased in S phase. Moreover, the expression of phosphorylated Akt was down-regulated by CK2alpha silencing.


CONCLUSIONS
Protein kinase CK2alpha plays an important role in the proliferation and invasion of nasopharyngeal carcinoma, and may provide a potential therapeutic target against nasopharyngeal cancer.
Liu
Li
L
Department of Health Management, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Ding
Yan-qing
YQ
D047390:Casein Kinase II / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034741:RNA, Small Interfering; D047390:Casein Kinase II; D051057:Proto-Oncogene Proteins c-akt