Frequency of bacille Calmette-Guerin (BCG) and Mycobacterium tuberculosis in tissue biopsy specimens of children vaccinated with BCG.
Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the mycobacterial species. The overall sensitivity of the assay was 71.5%. The prevalence rates of BCG, Mycobacterium tuberculosis, and other mycobacteria in the positive results were 80% (12/15), 13% (2/15), and 7% (1/15), respectively.
Monajemzadeh
Maryam
M
Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.
Shahsiah
Reza
R
Zarei
Abdolmajid
A
Alamooti
Alireza Alai
AA
Mahjoub
Fatemeh
F
Mamishi
Setareh
S
Khotai
Ghamartaj
G
Pazira
Reza
R
Eram
Neda
N
D001500:BCG Vaccine / Q000008:administration & dosage*; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006785:Hospitals, University; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007492:Iran / Q000453:epidemiology; D008297:Male; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology*
D001500:BCG Vaccine; D004269:DNA, Bacterial