title: Endothelial bioreactor ameliorates endotoxemia sepsis in swine.
abstract: BACKGROUND
  Severe sepsis involves a complex response including the activation of lots of cells, inflammatory mediators, and the hemostatic system. Central to this process is an alteration of endothelial cell function. Therefore, we investigated whether an endothelial bioreactor (EBR) would provide a new therapeutic approach to this clinical disorder.
  
  
  METHODS
  EBR was constructed using a cartridge which contained with nonwoven polytetrafluoroethylene seeded with porcine iliac artery endothelial cell (PIEC). Pigs were intravenously administered with 0.25 mg/kg lipopolysaccharide and immediately placed in an extracorporeal circuit with EBR or sham controls.
  
  
  RESULTS
  Compared with the sham group, EBR therapy resulted in significantly higher mean arterial blood pressure and significantly lower plasma von Willebrand factor, endothelin-1 and scores of lung injury. These alterations were associated with a significant survival advantage in the EBR group.
  
  
  CONCLUSIONS
  Timely intervention with EC therapy in a tissue-engineered bioreactor may improve cardiovascular performance and alter the natural history of endotoxemia sepsis.
authors.affiliation: Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, PR China.
authors.forename: Qionghong
authors.initials: Q
authors.lastname: Xie
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.forename: Yi
authors.initials: Y
authors.lastname: Ping
authors.forename: Li
authors.initials: L
authors.lastname: You
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zhong
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Hyo
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Weimin
authors.initials: W
authors.lastname: Liang
authors.forename: Shanyan
authors.initials: S
authors.lastname: Lin
authors.forename: Feng
authors.initials: F
authors.lastname: Ding
mesh_terms: D000818:Animals; D019149:Bioreactors / Q000662:veterinary*; D001794:Blood Pressure; D042783:Endothelial Cells; D019332:Endothelin-1 / Q000097:blood; D019446:Endotoxemia / Q000473:pathology / Q000628:therapy*; D008168:Lung / Q000473:pathology; D018805:Sepsis / Q000473:pathology / Q000628:therapy*; D013552:Swine; D023822:Tissue Engineering; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D014841:von Willebrand Factor