Mutational analysis of the PITX2 and NKX2-5 genes in patients with idiopathic atrial fibrillation.
Atrial fibrillation (AF) is the most frequently encountered arrhythmia in clinical practice. In a subgroup of patients, AF is regarded as idiopathic when no signs of structural heart disease or other causes of the arrhythmia can be identified during conventional clinical work-up. Recent studies have demonstrated that AF has a substantial genetic basis in a number of cases. The entire coding sequences, including intron-exon boundaries, of the genes PITX2 and NKX2-5 were screened for genetic variants by means of initial polymerase chain reaction followed by DNA sequencing in 96 patients with idiopathic AF. Although we detected a number of variants, our candidate gene approach did not result in identification of mutations associated with AF in the coding regions of PITX2 or NKX2-5 in our well characterized AF cohort.
Boldt
Leif-Hendrik
LH
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: leif-hendrik.boldt@charite.de.
Posch
Maximilian G
MG
Charité-Universitätsmedizin Berlin, Experimental and Clinical Research Center (ECRC), Lindenberger Weg 80, 13125 Berlin, Germany.
Perrot
Andreas
A
Charité-Universitätsmedizin Berlin, Experimental and Clinical Research Center (ECRC), Lindenberger Weg 80, 13125 Berlin, Germany.
Polotzki
Michael
M
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
Rolf
Sascha
S
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
Parwani
Abdul S
AS
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
Huemer
Martin
M
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
Wutzler
Alexander
A
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
Özcelik
Cemil
C
Charité-Universitätsmedizin Berlin, Experimental and Clinical Research Center (ECRC), Lindenberger Weg 80, 13125 Berlin, Germany.
Haverkamp
Wilhelm
W
Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Department of Cardiology, Augustenburger Platz 1, 13353 Berlin, Germany.
D000368:Aged; D001281:Atrial Fibrillation / Q000175:diagnosis / Q000235:genetics*; D015331:Cohort Studies; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014157:Transcription Factors / Q000235:genetics*; D000097577:Homeobox Protein PITX2
D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2; C095552:NKX2-5 protein, human