title: Structure and genome organization of the large RNA of iris yellow spot virus (genus Tospovirus, family Bunyaviridae).
abstract: The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame in the viral complementary (vc) strand. The primary translation product of 331.17 kDa shared many of the features of the viral RNA-dependent RNA polymerase (RdRp) coded by L RNAs of known tospoviruses. The 5' and 3' termini of IYSV L RNA (vc) contain two untranslated regions of 33 and 226 nucleotides, respectively, and both termini have conserved terminal nucleotides, another common feature of tospovirus genomic RNAs. Conserved motifs characteristic of RdRps of members of the family Bunyaviridae were present in the IYSV RdRp.
authors.affiliation: Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430, USA.
authors.forename: Sudeep
authors.initials: S
authors.lastname: Bag
authors.forename: K L
authors.initials: KL
authors.lastname: Druffel
authors.forename: H R
authors.initials: HR
authors.lastname: Pappu
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D023061:Gene Order; D005814:Genes, Viral; D007498:Iris / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017865:Tospovirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase