Biosynthesis of proteins inside liposomes.
Protein expression is the most complex metabolic reaction that has been encapsulated in liposomes, mainly as an intermediate step toward the synthesis of minimal semisynthetic cells. Although there are different experimental approaches to achieving the synthesis of proteins inside liposomes and it is therefore not possible to give a standard recipe, all methods follow a general strategy, which is briefly discussed. On this basis, we provide general indications for designing and realizing protein-expressing liposomes. Our approach for the green fluorescent protein expression inside 200-nm extruded vesicles is described in detail.
Stano
Pasquale
P
Biology Department, University of RomaTre, Rome, Italy.
Kuruma
Yutetsu
Y
Souza
Tereza Pereira
TP
Luisi
Pier Luigi
PL
D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*
D008081:Liposomes; D011506:Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins