title: Enhanced stability and in vitro bioactivity of surfactant-loaded liposomes containing Asiatic Pennywort extract.
abstract: The objective of this work has been the microencapsulation of Asiatic Pennywort (AP) extract with lecithin from soybean. The effect of various quantities of non-ionic surfactant (Montanov82) on liposomes upon physicochemical characteristics as well as their in vitro bio-activities was investigated. An addition of surfactant resulted in a decrease in particle size and an increase in percentage AP entrapment efficiency of liposomes. The surfactant-loaded liposomes demonstrated higher stability than surfactant-free liposomes where higher percentage AP remaining of liposomes can be achieved depending on surfactant concentration. No significant difference was found on AP release profiles among varied surfactant concentrations, although a presence of surfactant resulted in prolonged AP release rate. Liposomal AP with 20% w/w surfactant or higher demonstrated low cytotoxicity, a stronger anti-oxidation effect and collagen production on dermal fibroblast cells when compared with free AP and surfactant-free liposomes, possibly due to better cell internalization and less AP degradation in cells.
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand.
authors.forename: Somsak
authors.initials: S
authors.lastname: Saesoo
authors.forename: Issara
authors.initials: I
authors.lastname: Sramala
authors.forename: Apinan
authors.initials: A
authors.lastname: Soottitantawat
authors.forename: Tawatchai
authors.initials: T
authors.lastname: Charinpanitkul
authors.forename: Uracha Rungsardthong
authors.initials: UR
authors.lastname: Ruktanonchai
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D028041:Centella / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D004339:Drug Compounding; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D054709:Lecithins / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry; D013501:Surface-Active Agents
chemical_list: D000975:Antioxidants; D054709:Lecithins; D008081:Liposomes; D010936:Plant Extracts; D013501:Surface-Active Agents; D003094:Collagen