Role of untranslated regions in regulation of gene expression, replication, and pathogenicity of Newcastle disease virus expressing green fluorescent protein.
To gain insight into the role of untranslated regions (UTRs) in regulation of foreign gene expression, replication, and pathogenicity of Newcastle disease virus (NDV), a green fluorescent protein (GFP) gene flanked by 5' and 3' UTRs of each NDV gene was individually expressed by recombinant NDVs. UTRs of each gene modulated GFP expression positively or negatively. In particular, UTRs of the M and F genes enhanced levels of GFP expression at the junction of the P and M genes without altering replication of NDV, suggesting that UTRs could be used for enhanced expression of a foreign gene by NDV.
Kim
Shin-Hee
SH
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA.
Samal
Siba K
SK
D000818:Animals; D002642:Chick Embryo; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D009522:Newcastle disease virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020506:Untranslated Regions; D014771:Virion / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D020506:Untranslated Regions; D049452:Green Fluorescent Proteins