The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats.
OBJECTIVE
To investigate the effect of prophylactic dose of a low molecular weight heparin, enoxaparin, on skin wound healing of rats.


METHODS
Forty rats were used for the study. Rats were randomly assigned to two equal groups. Experimental group received prophylactic dose of enoxaparin. Physiologic saline was administered to the control group. Parameters of wound healing of experimental and control groups were compared. For comparison of the groups in terms of fibrosis, vascularization, inflammation, epithelization, and tensile strength test (Newton). Mann-Whitney-U test was used because variables were categorical data (fibrosis, vascularization, inflammation and epithelization). Differences between groups were analyzed with independent samples t-test (tensile strength). Significance was set at p<0.05.


RESULTS
Skin wound of the experimental group presented tensile strength significantly decreased (p<0.001), histopathologic examination revealed a significant (p<0.001) delayed epithelization and decreased in fibrosis, vascularization, inflammation (p<0.001) in the experimental group.


CONCLUSIONS
Enoxaparin delay wound healing by decreased inflammatory cells, fibroblast contents and their products (growth factors), and by promoted hemorrhage.
Oken
Ozdamar Fuad
OF
Experimental Animal Laboratory, Faculty of Medicine, University of Ankara, Yenimahalle, Ankara, Turkey. fuadoken@yahoo.com
Yildirim
Ahmet Ozgur
AO
Gulcek
Murat
M
Unal
Vuslat Sema
VS
Karakuyu
Akin
A
Ozlu
Korhan
K
Ucaner
Ahmet
A
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D017984:Enoxaparin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D018709:Statistics, Nonparametric; D013718:Tensile Strength / Q000187:drug effects; D020246:Venous Thrombosis / Q000517:prevention & control*; D014945:Wound Healing / Q000187:drug effects*
D000925:Anticoagulants; D017984:Enoxaparin