title: Indirect immunofluorescent labeling of fixed cells.
abstract: Flow cytometry protocols for defining cell surface or intracellular antibody staining are discussed. Various staining protocols are provided. Routine cell surface and intracellular techniques as well as more advanced signal enhancement techniques are detailed.
authors.affiliation: Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D005404:Fixatives; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D017830:Octoxynol; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D000954:Antigens, Surface; D005404:Fixatives; D017830:Octoxynol