Clinical relevance of the TLR4 11367 polymorphism in patients with major trauma.
OBJECTIVE
To investigate the clinical relevance of the TLR4 11367 polymorphism in patients with major trauma.


METHODS
Genetic functional and association study.


METHODS
Daping Hospital and Chongqing Emergency Medical Center, Chongqing, China.


METHODS
A total of 132 patients with major trauma were prospectively recruited.


METHODS
The TLR4 11367 polymorphism was genotyped using single-tube, bidirectional, allele-specific amplification method. Whole peripheral blood samples obtained within 24 hours after admission were stimulated with lipopolysaccharide and then tested for production of tumor necrosis factor alpha and interleukin 6. Sepsis morbidity rate and multiple organ dysfunction scores were assessed.


RESULTS
The 11367 polymorphism was shown to be strongly associated with less capacity of peripheral leukocytes to produce tumor necrosis factor alpha and interleukin 6 in response to ex vivo lipopolysaccharide stimulation in patients with trauma at admission. Results from association study indicated that patients with trauma who carry the 11367C allele were less likely to have sepsis and multiple organ dysfunction.


CONCLUSIONS
Combined with our previous in vitro functional study, the results suggest that the TLR4 11367 polymorphism might be a good predictor of who is more likely to develop complications such as sepsis or multiple organ dysfunction syndrome, depending on genotype.
Duan
Zhao-xia
ZX
State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Gu
Wei
W
Zhang
Lian-yang
LY
Du
Ding-yuan
DY
Hu
Ping
P
Huang
Jian
J
Liu
Qing
Q
Wang
Zheng-guo
ZG
Hao
Jiang
J
Jiang
Jian-Xin
JX
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D002681:China; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D018805:Sepsis / Q000453:epidemiology / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014947:Wounds and Injuries / Q000150:complications* / Q000235:genetics*; D055815:Young Adult
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4