Deficiencies in elements involved in TLR4-receptor signalling in RBL-2H3 cells.
OBJECTIVE
RBL-2H3 cells express Toll-like receptors, including TLR4. This study aims to assess various aspects of the TLR4 pathway.


METHODS
RBL-2H3 cells were indirectly stained for cell surface TLR4, 25 CD14 and intracellular MyD88 proteins and analysed through flow cytometry for single-colour staining.


RESULTS
While TLR4-receptors are expressed in RBL-2H3 cells, associated elements involved in the signaling pathway, CD14 and MyD88, are not.


CONCLUSIONS
Care should be taken if RBL-2H3 cells are used to study aspects of the innate immune system in mast cells.
Passante
E
E
School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland.
Frankish
N
N
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000502:physiology*; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D010457:Peptidoglycan / Q000494:pharmacology; D051381:Rats; D019779:Salmonella enterica / Q000737:chemistry; D015398:Signal Transduction / Q000502:physiology*; D051197:Toll-Like Receptor 4 / Q000502:physiology*
D005456:Fluorescent Dyes; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; D010457:Peptidoglycan; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4