Mycosporine-like amino acids in freshwater copepods: potential sources and some factors that affect their bioaccumulation.
Mycosporine-like amino acids (MAAs) are ubiquitous photoprotective compounds in aquatic environments. MAAs are synthesized by a wide variety of organisms (i.e. bacteria, fungi and algae) and their production is photoinducible by ultraviolet radiation (UVR) (280-400 nm) and/or photosynthetically active radiation (400-750 nm). Most animals however, are unable to synthesize MAAs and must acquire these compounds through their diet or from symbiotic organisms. In this paper, we investigate the possible sources of MAAs and factors (temperature and initial MAA concentration) that may affect their bioaccumulation in freshwater copepods. We found that MAA accumulation may occur even if the copepods are cultured on a MAA-free diet. In addition, we found that the bacteriostatic antibiotic, chloramphenicol, inhibits the bioaccumulation of MAAs. These two pieces of evidence suggest that the source of MAAs in these copepods may be prokaryotic organisms in close association with the animals. The two factors investigated in this study, temperature and initial MAA concentrations, were found to affect the rates at which MAAs are accumulated. Temperature had positive effects on both uptake and elimination rates. On the other hand, the rate of uptake decreased at the highest assayed initial MAA concentration, probably because the concentration of MAAs was already close to saturation.
García
Patricia E
PE
Laboratorio de Fotobiología, Instituto en Investigaciones de Biodiversidad y Medioambiente (INIBIOMA), Universidad Nacional del Comahue, Río Negro, Argentina.
Diéguez
María C
MC
Ferraro
Marcela A
MA
Zagarese
Horacio E
HE
Pérez
Alejandra P
AP
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D033342:Copepoda / Q000378:metabolism*; D003511:Cyclohexanols / Q000378:metabolism*; D004032:Diet; D020387:Food Chain; D005618:Fresh Water; D011837:Radiation-Protective Agents / Q000378:metabolism*; D014466:Ultraviolet Rays
D000596:Amino Acids; D003511:Cyclohexanols; D011837:Radiation-Protective Agents