title: Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp.
abstract: Ecklonia cava is a brown alga (Laminariales, Phaeophyta) growing on the subtidal rocky shores of Korea. It has antioxidant, antidiarrhea, and anticoagulant effects. In this study, the antimicrobial activity of E. cava EtOH extract and its fractions (n-hexane, CH2Cl2, EtOAc, n-BuOH, and H2O) were investigated against methicillin-resistant Staphylococcus aureus and Salmonella spp. The E. cava EtOAc fraction showed good antibacterial activity against all bacteria. Eckol isolated from E. cava EtOAc fraction showed antimicrobial activity against all the tested strains. The minimum inhibitory concentration of eckol against S. aureus strains ranged from 125 to 250 microg/mL and 125 to 250 microg/mL for Salmonella strains. The fraction inhibitory concentration index of eckol in combination with ampicillin ranged from 0.31 to 0.5, indicating remarkable synergism against S. aureus. However, against Salmonella gallinarum ATCC 9184 and Salmonella typhimurium, it ranges from 0.75 to 1.0. The combinations of eckol + ampicillin exhibited improved inhibition of S. aureus and Salmonella with synergy or additive effect. We suggest that eckol ingredients of the E. cava against S. aureus and Salmonella have antibacterial activity.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk, Republic of Korea.
authors.forename: Jang-Gi
authors.initials: JG
authors.lastname: Choi
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Obiang-Obounou
authors.initials: OO
authors.lastname: Brice
authors.forename: Young-Seob
authors.initials: YS
authors.lastname: Lee
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Chae
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Oh
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Han-Gil
authors.initials: HG
authors.lastname: Choi
authors.forename: Se-Gun
authors.initials: SG
authors.lastname: Kim
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Shin
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000465:Algorithms; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005591:Chemical Fractionation; D004147:Dioxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004357:Drug Synergism; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry*; D012475:Salmonella / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects; D012997:Solvents; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004147:Dioxins; D012997:Solvents; D000667:Ampicillin; C060311:eckol