Composition and dynamics of sulfate-reducing bacteria during the waterflooding process in the oil field application.
The composition and dynamics of sulfate-reducing bacteria (SRB) during the waterflooding process of Daqing Oilfield were investigated in this study. PCR-DGGE analysis indicated that the microbial communities were significantly different in each treatment unit, and the dominant members were mainly close to Clostridium sp., Thauera sp., Hydrogenophaga sp., Pseudomonas sp., Eubacterium sp. and Arcobacter sp. However, the members belonging to SRB were relatively few and mainly consisted of Desulfovibrio sp. and Desulfovibrio profundus. According to APS (adenosine-5'-phosphosulfate reductase) gene clone library and sequence analysis, it was found that SRB mainly belonged to Proteobacteria and Deltaproteobacteria. In the library, the genus Desulfovibrio of Deltaproteobacteria was about 65.2% while genus Desulfomonas, Desulfomicrobium, Desulfohalobium, Desulfonatronum, Desulfbulbus, Desulforhopalus, Desulfnema and Desulforhopalus were occupied nearly 34.8%. It was also proved that most of SRB in the oil field were enriched in the ground treatment unit and they are not in the liquid of oil well.
Wei
Li
L
State Key Lab of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Ma
Fang
F
Zhao
Guang
G
D001483:Base Sequence; D002681:China; D017931:DNA Primers / Q000235:genetics; D020564:Deltaproteobacteria / Q000235:genetics / Q000378:metabolism*; D004586:Electrophoresis; D019652:Extraction and Processing Industry; D008969:Molecular Sequence Data; D010578:Petroleum; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism; D014871:Water Microbiology
D017931:DNA Primers; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D013431:Sulfates