Effects of Yangtze River source water on genomic polymorphisms of male mice detected by RAPD.
In order to evaluate the environmental health risk of drinking water from Yangtze River source, randomly amplified polymorphic DNA (RAPD) markers were used to detect the effects of the source water on genomic polymorphisms of hepatic cell of male mice (Mus musculus, ICR). After the mice were fed with source water for 90 days, RAPD-polymerase chain reactions (PCRs) were performed on hepatic genomic DNA using 20 arbitrary primers. Totally, 189 loci were generated, including 151 polymorphic loci. On average, one PCR primer produced 5.3, 4.9 and 4.8 bands for each mouse in the control, the groups fed with source water and BaP solution, respectively. Compared with the control, feeding mice with Yangtze River source water caused 33 new loci to appear and 19 to disappear. Statistical analysis of RAPD printfingers revealed that Yangtze River source water exerted a significant influence on the hepatic genomic polymorphisms of male mice. This study suggests that RAPD is a reliable and sensitive method for the environmental health risk of Yangtze River source water.
Zhang
Xiaolin
X
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China. zhangxx@nju.edu.cn
Zhang
Zongyao
Z
Zhang
Xuxiang
X
Wu
Bing
B
Zhang
Yan
Y
Yang
Liuyan
L
Cheng
Shupei
S
D000818:Animals; D002681:China; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004781:Environmental Exposure / Q000009:adverse effects*; D004784:Environmental Monitoring / Q000379:methods; D056426:Genetic Loci / Q000187:drug effects; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000187:drug effects*; D019105:Random Amplified Polymorphic DNA Technique; D018570:Risk Assessment / Q000379:methods; D045483:Rivers; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D014873:Water Pollutants / Q000633:toxicity*; D014881:Water Supply
D014873:Water Pollutants; D004247:DNA