Differential effects of natural and synthetic vitamin E on gene transcription in murine T lymphocytes.
Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differences between the two vitamin forms in regulating gene expression in response to T-cell stimulation. Marker genes have been found whose expression can be considered significant in establishing the level of, and response to vitamin E for both natural and synthetic vitamin E supplementation; unique markers for synthetic vitamin E supplementation and unique markers for natural vitamin E supplementation have been identified.
Han
Sung Nim
SN
Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul, South Korea.
Pang
Eunice
E
Zingg
Jean-Marc
JM
Meydani
Simin Nikbin
SN
Meydani
Mohsen
M
Azzi
Angelo
A
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D016678:Genome; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D024505:Tocopherols / Q000378:metabolism / Q000494:pharmacology; D014810:Vitamin E / Q000378:metabolism* / Q000494:pharmacology*
D014810:Vitamin E; D024505:Tocopherols