Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals.
OBJECTIVE
The purpose of this study was to evaluate the action of sodium hypochlorite (NaOCl) associated with an intracanal medication against Candida albicans and Enterococcus faecalis inoculated in root canals.


METHODS
Thirty-six human single-rooted teeth with single root canals were used. The canals were contaminated with C. albicans and E. faecalis for 21 days and were then instrumented with 1% NaOCl. The roots were divided into 3 groups (n=12) according to the intracanal medication applied: calcium hydroxide paste, 2% chlorhexidine (CHX) gel, and 2% CHX gel associated with calcium hydroxide. The following collections were made from the root canals: a) initial sample (IS): 21 days after contamination (control), b) S1: after instrumentation, c) S2: 14 days after intracanal medication placement; S3: 7 days after intracanal medication removal. The results were analyzed statistically by the Kruskal-Wallis test at 5% significance level.


CONCLUSIONS
Both 1% NaOCl irrigation and the intracanal medications were effective in eliminating E. faecalis and C. albicans inoculated in root canals.
Valera
Marcia Carneiro
MC
Department of Restorative Dentistry, São Paulo State University, São José dos Campos, Brazil. marcia@fosjc.unesp.br
Silva
Katy Costa Godinho da
KC
Maekawa
Lilian Eiko
LE
Carvalho
Cláudio Antonio Talge
CA
Koga-Ito
Cristiane Yumi
CY
Camargo
Carlos Henrique Ribeiro
CH
Lima
Raphael Silva e
RS
D000890:Anti-Infective Agents / Q000627:therapeutic use*; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002176:Candida albicans / Q000187:drug effects*; D002710:Chlorhexidine / Q000627:therapeutic use; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D013997:Time Factors
D000890:Anti-Infective Agents; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide; D002710:Chlorhexidine