title: Administration of thyroid hormone increases reelin and brain-derived neurotrophic factor expression in rat hippocampus in vivo.
abstract: Thyroid hormones play important roles in the maturation and function of the central nervous system. However, the underlying mechanism behind thyroid hormone-regulated gene expression in the adult brain is not well understood. Two genes critical for neuronal plasticity and implicated in psychiatric disorders, reelin and brain-derived neurotrophic factor (BDNF), were investigated in the present study. Triiodothyronine (T3), the active form of thyroid hormone was administered to young adult rats in two different manners: systemic injection or local brain infusion. Real time RT-PCR results revealed that T3 administration lead to a significant increase in reelin, total BDNF and exon-specific BDNF mRNA expression in the hippocampus. Furthermore, the association of transcriptional coactivators (including steroid receptor coactivator-1 (SRC-1), cAMP response element binding protein-binding protein (CBP), and thyroid hormone receptor associated protein 220 (TRAP 220)) and RNA polymerase II (RNA Pol II), with reelin and BDNF genes in the rat hippocampus displayed a distinct process following thyroid hormone administration. These findings suggest that association of transcriptional coactivators and RNA Pol II with gene promoters may be a possible mechanism explaining T3-induced reelin and BDNF expression in the hippocampus of young adult rats.
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. lsui@fudan.edu.cn
authors.forename: Li
authors.initials: L
authors.lastname: Sui
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Ren
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D050882:CREB-Binding Protein / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D047468:Immunoprecipitation; D008297:Male; D056913:Mediator Complex Subunit 1 / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D056920:Nuclear Receptor Coactivator 1 / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091042:Reelin Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000378:metabolism*; D013963:Thyroid Hormones / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D014284:Triiodothyronine / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D056913:Mediator Complex Subunit 1; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000091042:Reelin Protein; C000717129:Reln protein, rat; D013963:Thyroid Hormones; D014284:Triiodothyronine; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat; D056920:Nuclear Receptor Coactivator 1; D012319:RNA Polymerase II; D012697:Serine Endopeptidases