Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy.
We report optical-resolution photoacoustic microscopy (OR-PAM) for in vivo imaging of amyloid plaques in an Alzheimer's disease mouse model. Validation using conventional fluorescence microscopy and multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window without angiographic contrast agents. OR-PAM, capable of providing both exogenous molecular contrast and endogenous hemoglobin contrast, has the potential to serve as a new technology for in vivo microscopic observations of cerebral plaque deposits.
Hu
Song
S
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
Yan
Ping
P
Maslov
Konstantin
K
Lee
Jin-Moo
JM
Wang
Lihong V
LV
D000544:Alzheimer Disease / Q000473:pathology*; D000818:Animals; D001921:Brain / Q000473:pathology*; D004195:Disease Models, Animal; D054459:Elasticity Imaging Techniques / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity