title: Basal body stability and ciliogenesis requires the conserved component Poc1.
abstract: Centrioles are the foundation for centrosome and cilia formation. The biogenesis of centrioles is initiated by an assembly mechanism that first synthesizes the ninefold symmetrical cartwheel and subsequently leads to a stable cylindrical microtubule scaffold that is capable of withstanding microtubule-based forces generated by centrosomes and cilia. We report that the conserved WD40 repeat domain-containing cartwheel protein Poc1 is required for the structural maintenance of centrioles in Tetrahymena thermophila. Furthermore, human Poc1B is required for primary ciliogenesis, and in zebrafish, DrPoc1B knockdown causes ciliary defects and morphological phenotypes consistent with human ciliopathies. T. thermophila Poc1 exhibits a protein incorporation profile commonly associated with structural centriole components in which the majority of Poc1 is stably incorporated during new centriole assembly. A second dynamic population assembles throughout the cell cycle. Our experiments identify novel roles for Poc1 in centriole stability and ciliogenesis.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA. Chad.Pearson@Colorado.edu
authors.forename: Chad G
authors.initials: CG
authors.lastname: Pearson
authors.forename: Daniel P S
authors.initials: DP
authors.lastname: Osborn
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Giddings
authors.forename: Philip L
authors.initials: PL
authors.lastname: Beales
authors.forename: Mark
authors.initials: M
authors.lastname: Winey
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002502:Centrioles / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D013696:Temperature; D016808:Tetrahymena thermophila / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008840:Microfilament Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D029961:Zebrafish Proteins