title: Design of natural food antioxidant ingredients through a chemometric approach.
abstract: In the present work, an environmentally friendly extraction process using subcritical conditions has been tested to obtain potential natural food ingredients from natural sources such as plants, fruits, spirulina, propolis, and tuber, with the scope of substituting synthetic antioxidants, which are subject to regulation restrictions and might be harmful for human health. A full characterization has been undertaken from the chemical and biochemical point of view to be able to understand their mechanism of action. Thus, an analytical method for profiling the compounds responsible for the antioxidant activity has been used, allowing the simultaneous determination of water-soluble vitamins, fat-soluble vitamins, phenolic compounds, carotenoids, and chlorophylls in a single run. This information has been integrated and analyzed using a chemometrical approach to correlate the bioactive compounds profile with the antioxidant activity and thus to be able to predict antioxidant activities of complex formulations. As a further step, a simplex centroid mixture design has been tested to find the optimal formulation and to calculate the effect of the interaction among individual extracts in the mixture.
authors.affiliation: Instituto de Fermentaciones Industriales, 28006 Madrid, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Mendiola
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Martín-Alvarez
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Señoráns
authors.forename: Guillermo
authors.initials: G
authors.lastname: Reglero
authors.forename: Alessandro
authors.initials: A
authors.lastname: Capodicasa
authors.forename: Filomena
authors.initials: F
authors.lastname: Nazzaro
authors.forename: Alfonso
authors.initials: A
authors.lastname: Sada
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
authors.forename: Elena
authors.initials: E
authors.lastname: Ibáñez
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry*; D002338:Carotenoids / Q000032:analysis; D005503:Food Additives / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D053545:Spirulina / Q000737:chemistry*; D014815:Vitamins / Q000032:analysis
chemical_list: D000975:Antioxidants; D001688:Biological Products; D005503:Food Additives; D010636:Phenols; D010936:Plant Extracts; D014815:Vitamins; D002338:Carotenoids