O-beta-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots.
BACKGROUND
Italian air force acrobatic pilots are occupationally susceptible to oxidative stress damage that can lead to overt signs and symptoms of hypoxia. We propose erythrocyte glycohydrolases as new, sensitive markers to assess oxidative stress.


METHODS
We measured erythrocyte concentrations of beta-D-glucuronidase (GCR), hexosaminidase, O-beta-N-acetyl-D-glucosaminidase (O-GlcNAcase), plasma membrane fluidity and plasma hydroperoxides from 19 pilots and compared these to 40 matched healthy subjects.


RESULTS
Plasma hydroperoxide concentrations and the erythrocyte ghosts' fluorescence anisotropy were significantly lower in the pilots. Concentrations of GCR, O-GlcNAcase and hexosaminidase in pilots were significantly different from controls, being lower, higher and higher, respectively.


CONCLUSIONS
Pilots, in spite of their oxidative stress, are better protected than controls, probably as a result of their physical training and proper diet. Our results confirm that erythrocytes, with their 120-day life span, are a useful model for investigating physiopathological conditions, and glycohydrolases are good markers for monitoring oxidative stress, even in healthy people.
Corsi
Massimiliano M
MM
Department of Human Morphology and Biomedical Sciences Città Studi, Laboratory of Clinical Pathology, University of Milan, Milan, Italy.
Massaccesi
Luca
L
Dogliotti
Giada
G
Vianello
Elena
E
Agrifoglio
Marco
M
Palumbo
Fabrizio
F
Goi
Giancarlo
G
D000118:Acetylglucosaminidase / Q000097:blood* / Q000378:metabolism; D000328:Adult; D000401:Aircraft; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005454:Fluorescence Polarization; D006596:Hexosaminidases / Q000097:blood* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood* / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D008889:Military Personnel; D016273:Occupational Exposure; D018384:Oxidative Stress; D013997:Time Factors; D055815:Young Adult
D015415:Biomarkers; D006861:Hydrogen Peroxide; D006596:Hexosaminidases; D000118:Acetylglucosaminidase