Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure.
The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-->4-alpha-D-Glcp-(1-->3)-alpha-L-FucpNAc-(1-->3)-[alpha-D-Glcp-(1-->4)]-alpha-D-GlcpNAc-(1-->3)-alpha-L-FucpNAc-(1-->4)-alpha-D-Glcp-(1-->P}(n).
Niedziela
Tomasz
T
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland. tomasz.niedziela@iitd.pan.wroc.pl
Kenne
Lennart
L
Lugowski
Czeslaw
C
D002240:Carbohydrate Sequence; D020611:Hafnia alvei / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013682:Teichoic Acids / Q000737:chemistry*
D019081:O Antigens; D013682:Teichoic Acids