title: [Experimental study of vitreoretinal proliferation inhibition by the use of hypericin].
abstract: OBJECTIVE
  To produce proliferative vitreoretinopathy (PVR) in an animal ocular trauma model. To evaluate the inhibition of (PVR) emergence and progression by hypericin.
  
  
  METHODS
  Experimental Study. Nineteen pigmented male adult rabbits weighing between 2,000 and 3,000 grams were used in this study. All of them were submitted to trauma model with dispase and retinal diathermy to induce PVR membranes formation. They were randomly assigned to receive hypericin (10 microM in 0.1 ml) or saline solution (0.1 ml) as placebo. They were evaluated clinically in the seventh, fourteenth, twenty-first and twenty-eighth postoperative days with indirect ophthalmoscopy and digital color retinography. The PVR degree was classified according to Hida (0 to 7).
  
  
  RESULTS
  Membranes formation was present in 79% of the eyes; being 100% in the eyes of placebo group and 60% in the eyes of treatment group (hypericin). The comparison between PVR phases averages within the groups showed a statistically significant difference between the two groups, with a p value of 0.0321 for Wilcoxon test.
  
  
  CONCLUSIONS
  The trauma model with dispase and retinal diathermy produces vitreoretinal membranes. Hypericin was considered effective in PVR emergence and progression decrease.
authors.affiliation: Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Roberto Augusto Fernandes
authors.initials: RA
authors.lastname: Machado
authors.forename: Antonio Marcelo Barbante
authors.initials: AM
authors.lastname: Casella
authors.forename: Marcus Rudolph
authors.initials: MR
authors.lastname: Malaguido
authors.forename: Ana Paula Miyagusko Taba
authors.initials: AP
authors.lastname: Oguido
mesh_terms: D000818:Animals; D000873:Anthracenes; D010450:Endopeptidases / Q000008:administration & dosage; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D011817:Rabbits; D012160:Retina / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D018630:Vitreoretinopathy, Proliferative / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000873:Anthracenes; D004791:Enzyme Inhibitors; D010569:Perylene; C004965:hypericin; D010450:Endopeptidases; C023139:dispase