title: Cytomegalovirus glycoprotein B genotype in hematopoietic stem cell transplant patients from China.
abstract: Cytomegalovirus (CMV) can be classified into 4 subgroups based on genotype variation of the glycoprotein B (gB) encoded by UL55 gene. Little is known about the CMV gB genotype distribution and its clinical implication in patients who receive hematopoietic stem cell transplant (HSCT) in China. In this study, which comprises 101 HSCT patients with CMV infection, we have found that 36 patients (35.64%) were infected with CMV genotype gB1, 3 patients (2.97%) with gB2, 39 patients (38.61%) with gB3, 1 patient (0.99%) with gB4, and 17 patients (16.83%) were infected with mixed CMV genotypes. We also found that CMV gB3 was associated with a high risk of CMV pneumonitis; nevertheless, there were no significant differences among patients with gB genotypes with respect to the other CMV diseases; no significant differences between patients with gB genotypes with respect to type II-IV acute graft-verses-host disease (aGVHD) and chronic GVHD (cGVHD) was found either. Interestingly, 5 patients (4.95%) were infected with a CMV variant that lacked a signature RsaI digestion site determined by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), subsequent cloning and sequencing identified this CMV RsaI minus variant to be novel, herein designated as gB5. Overall, these findings suggest that CMV gB1 and gB3 are prevalent among HSCT recipients with CMV infections, and gB3 CMV infection is a risky indicator for CMV pneumonitis; furthermore, a novel CMV variant in a subset of Chinese HSCT recipients is identified and designated as gB5.
authors.affiliation: Department of Hematology, First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology and Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou, People's Republic of China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Depei
authors.initials: D
authors.lastname: Wu
authors.forename: Aining
authors.initials: A
authors.lastname: Sun
authors.forename: Ziling
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.forename: Huiying
authors.initials: H
authors.lastname: Qiu
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tang
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Fu
authors.forename: Guangsheng
authors.initials: G
authors.lastname: He
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000209:etiology; D005260:Female; D005838:Genotype; D006086:Graft vs Host Disease / Q000821:virology; D019337:Hematologic Neoplasms / Q000150:complications / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000821:virology; D015995:Prevalence; D014759:Viral Envelope Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus