title: Kinetics and mechanisms of p-nitrophenol biodegradation by Pseudomonas aeruginosa HS-D38.
abstract: The kinetics and mechanisms of p-nitrophenol (PNP) biodegradation by Pseudomonas aeruginosa HS-D38 were investigated. PNP could be used by HS-D38 strain as the sole carbon, nitrogen and energy sources, and PNP was mineralized at the maximum concentration of 500 mg/L within 24 h in an mineral salt medium (MSM). The analytical results indicated that the biodegradation of PNP fit the first order kinetics model. The rate constant kPNP is 2.039 x 10(-2)/h in MSM medium, KPNP+N is 3.603 x 10(-2)/h with the addition of ammonium chloride and KPNP+C is 9.74 x 10(-3)/h with additional glucose. The addition of ammonium chloride increased the degradation of PNP. On the contrary, the addition of glucose inhibited and delayed the biodegradation of PNP. Chemical analysis results by thin-layer chromatography (TLC), UV-Vis spectroscopy and gas chromatography (GC) techniques suggested that PNP was converted to hydroquinone (HQ) and further degraded via 1,2,4-benzenetriol (1,2,4-BT) pathway.
authors.affiliation: Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Deli
authors.initials: D
authors.lastname: Liu
authors.forename: Shiwang
authors.initials: S
authors.lastname: Liu
authors.forename: Shangying
authors.initials: S
authors.lastname: Xu
authors.forename: Yongze
authors.initials: Y
authors.lastname: Yuan
authors.forename: Li
authors.initials: L
authors.lastname: Xiong
mesh_terms: D001673:Biodegradation, Environmental; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D009596:Nitrophenols; C024836:4-nitrophenol