Role of pyridoxamine in the formation of the Amadori/Heyns compounds and aggregates during the glycation of beta-lactoglobulin with galactose and tagatose.
The effect of pyridoxamine on the Maillard reaction during the formation of conjugates of beta-lactoglobulin with galactose and tagatose under controlled conditions (pH 7, 0.44 aw, 40 and 50 degrees C, for 6 days) has been studied, for the first time, by means of the changes in reducing carbohydrates, formation of Amadori or Heyns compounds, and aggregates and browning development. The results showed the formation of interaction products between pyridoxamine and galactose or tagatose either in the presence or in the absence of beta-lactoglobulin, indicating that pyridoxamine competes with the free amino groups of beta-lactoglobulin for the carbonyl group of both carbohydrates. Thus, a small inhibitory effect of pyridoxamine on the initial stages of the Maillard reaction was pointed out. Furthermore, much lower aggregation and color formation rates were observed in the conjugates of beta-lactoglobulin galactose/tagatose with pyridoxamine than without this compound, supporting its potent inhibitory effect on the advanced and final stages of the Maillard reaction. These findings reveal the usefulness of food-grade inhibitors of the advanced stages of the Maillard reaction, such as pyridoxamine, that, in combination with mild storage conditions, could lead to the formation of safer neoglycoconjugates without impairing their nutritional quality.
Corzo-Martínez
Marta
M
Instituto de Fermentaciones Industriales (CSIC), C/Juan de la Cierva, 3 28006 Madrid, Spain.
Moreno
F Javier
FJ
Olano
Agustín
A
Villamiel
Mar
M
D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D006601:Hexoses / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D015416:Maillard Reaction; D011733:Pyridoxamine / Q000737:chemistry*
D006601:Hexoses; D007782:Lactoglobulins; D011733:Pyridoxamine; C030192:tagatose; D005690:Galactose