Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes.
Cell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.
Boruc
Joanna
J
Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium.
Mylle
Evelien
E
Duda
Maria
M
De Clercq
Rebecca
R
Rombauts
Stephane
S
Geelen
Danny
D
Hilson
Pierre
P
Inzé
Dirk
D
Van Damme
Daniel
D
Russinova
Eugenia
E
D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D032461:Chromosomes, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D049452:Green Fluorescent Proteins