[Association of cytotoxic T lymphocyte antigen-4 promoter C-658T polymorphism with ulcerative colitis in Chinese].
OBJECTIVE
To investigate the association of gene polymorphism of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) with ulcerative colitis (UC) in Chinese.


METHODS
One hundred and seventeen patients with UC and 246 healthy controls were genotyped for the polymorphisms of C-658T in the promoter and C61T at the 3' untranslated region of the CTLA-4 gene using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), respectively. The genotype and allele frequencies of the two groups were calculated and compared by chi square test.


RESULTS
The frequency of TT+CT genotype at the CTLA-4 gene C-658T in the promoter was significantly higher in UC patients than that in healthy controls (P=0.015). The frequency of the T allele at this locus was also significantly higher in UC patients than that in the controls (P=0.033). The frequencies of TT genotype and T allele at the C-658T locus were highly associated with extensive colitis in UC patients (P=0.037, and P=0.0067, respectively).


CONCLUSIONS
The T allele of CTLA-4 promoter C-658T locus was highly associated with UC in Chinese Han of central China.
Luo
Yan
Y
Department of Gastroenterology, Zhongnan Hospital, Wuhan University School of Medicine, Wuhan, Hubei, 430071 P.R. China.
Xia
Bing
B
Li
Chun
C
Chen
Zhi-tao
ZT
Ge
Liu-qing
LQ
Jiang
Ting
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D060908:CTLA-4 Antigen; D016022:Case-Control Studies; D002681:China; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D055815:Young Adult
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human