title: Induction of papillary carcinoma in human ovarian surface epithelial cells using combined genetic elements and peritoneal microenvironment.
abstract: Papillary differentiation is one of the most common histological features of ovarian cancer, although the underlying mechanism that leads to such differentiation is not known. We hypothesized that human ovarian surface epithelial cells can be transformed into carcinoma with papillary differentiation by overexpressing HER2/neu in these cells. Mice were injected either subcutaneously or intraperitoneally with two immortalized human ovarian surface epithelial cell lines after enforced expression of HER-2/neu. Mice subcutaneously injected with tumor cells from either the T29Nt or T80Nt developed undifferentiated carcinomas. In contrast, mice injected intraperitoneally with T29Nt cells developed papillary carcinoma, and those injected intraperitoneally with T80Nt cells developed undifferentiated carcinoma. Our results demonstrate that ovarian surface epithelial cells can develop into papillary carcinoma in mice, and that the induction of papillary differentiation depends not only on specific genetic modifications but also on the tumor microenvironment and epithelial cell type from ovary from different patients.
authors.affiliation: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Zheng
authors.forename: Imelda
authors.initials: I
authors.lastname: Mercado-Uribe
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Rosen
authors.forename: Bin
authors.initials: B
authors.lastname: Chang
authors.forename: Peishu
authors.initials: P
authors.lastname: Liu
authors.forename: Gong
authors.initials: G
authors.lastname: Yang
authors.forename: Anais
authors.initials: A
authors.lastname: Malpica
authors.forename: Honami
authors.initials: H
authors.lastname: Naora
authors.forename: Nelly
authors.initials: N
authors.lastname: Auersperg
authors.forename: Gordon B
authors.initials: GB
authors.lastname: Mills
authors.forename: Robert C
authors.initials: RC
authors.lastname: Bast
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D010053:Ovary / Q000166:cytology*; D010537:Peritoneum / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000502:physiology
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases