title: Fibulin-3 negatively regulates chondrocyte differentiation.
abstract: Fibulin-3 is a member of the fibulin family that has been newly recognized as extracellular matrix proteins. We assessed the effects of fibulin-3 overexpression on chondrocyte differentiation using the clonal murine cell line ATDC5. The ATDC5-FBLN3 stably expressing fibulin-3 protein was spindle-shaped cell compared to the ATDC5-mock with plump cell. The cell growth in the ATDC5-FBLN3 was accelerated in comparison to that in the ATDC5-mock. The ATDC5-FBLN3 was not stained by Alcian blue, nor was there any cartilage aggregate formed after the induction of chondrogenic differentiation. The expression of type II collagen, aggrecan, and type X collagen was completely suppressed in ATDC5-FBLN3 even after the induction of differentiation. The overexpression of fibulin-3 reduced the expression of Sox5 and Sox6, while it maintained the expression of Sox9. These findings suggest that fibulin-3 may play an important role as a negative regulator of chondrocyte differentiation.
authors.affiliation: Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Matsumine
authors.forename: Shigeto
authors.initials: S
authors.lastname: Nakazora
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Iino
authors.forename: Hideki
authors.initials: H
authors.lastname: Ota
authors.forename: Hikaru
authors.initials: H
authors.lastname: Sonoda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sudo
authors.forename: Atsumasa
authors.initials: A
authors.lastname: Uchida
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011509:Proteoglycans / Q000096:biosynthesis; D055758:SOX9 Transcription Factor / Q000378:metabolism; D055755:SOXD Transcription Factors / Q000378:metabolism
chemical_list: C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D055758:SOX9 Transcription Factor; D055755:SOXD Transcription Factors; C089186:Sox5 protein, mouse; C095939:Sox6 protein, mouse