title: [Study on the differentially expressed genes of steroid-resistant and steroid-sensitive nephrotic syndrome].
abstract: OBJECTIVE
  To identify the genes differentially expressed among the steroid-resistant nephrotic syndrome (SRNS), steroid-sensitive nephrotic syndrome (SSNS) and normal children, and understand the molecular mechanism of SRNS.
  
  
  METHODS
  Affymetrix microarray technology was used to obtain such a profile. The differentially expressed genes among these groups were identified based on signal-to-noise ratios by GCOS software; real-time PCR analysis was performed to confirm the microarray results.
  
  
  RESULTS
  There were 157 genes differentially expressed among these groups. The genes up-regulated both in SRNS and SSNS were involved primarily in ionic transportation, immuno-signal transduction and apoptosis. In particular, CLNS1A gene was down regulated in SRNS but up regulated in SSNS.
  
  
  CONCLUSIONS
  Several differentially expressed genes, such as CLNS1A and HLA-DRB4 were found to be closely related to the pathogenesis of SRNS and SSNS. This DNA microarray analysis has provided some important clue to the molecular mechanism of SRNS.
authors.affiliation: Department of Pediatrics, the Affiliated Guangzhou Municipal People's Hospital of Guangzhou Medical College, Guangzhou, Guangdong, 510180 P.R. China.
authors.forename: Rong-yan
authors.initials: RY
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Hao
authors.forename: Li-na
authors.initials: LN
authors.lastname: Wang
authors.forename: Yue-qiang
authors.initials: YQ
authors.lastname: Wen
mesh_terms: D002648:Child; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000235:genetics*; D013256:Steroids / Q000627:therapeutic use*
chemical_list: D013256:Steroids