Purification of 125I-labelled lysine-vasopressin by affinity chromatography on sepharose-bound neurophysins.
Purification of 125I-labelled lysine-vasopressin has been achieved by affinity chromatography on a Sepharose 4 B conjugate of porcine neurophysins. This affinity absorbent did not retain halogenated hormone, while native lysine-vasopressin was bound on the solid support. The specific activity of purified iodinated lysine-vasopressin was 1700-1800 Ci/g, corresponding to one iodine atom per mole. By comparison with an unpurified tracer, a five times increase in the minimum detectable dose was obtained in the vasopressin radioimmunoassay.
Fressinaud
P H
PH
I.N.S.E.R.M. U-36 (Pr Housset), 1 7Rue du Fer à Moulin, 75005 Paris, France.
Corvol
P
P
Frenoy
J P
JP
Menard
J
J
D000818:Animals; D001671:Biochemistry / Q000379:methods; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007455:Iodine / Q000737:chemistry; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007668:Kidney / Q000378:metabolism; D008236:Lypressin / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry; D009481:Neurophysins / Q000737:chemistry*; D010902:Pituitary Gland / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D012685:Sepharose / Q000737:chemistry*; D013552:Swine
D007457:Iodine Radioisotopes; D009481:Neurophysins; D008236:Lypressin; D012685:Sepharose; D007455:Iodine; D008239:Lysine