Screening of closantel and rafoxanide in animal muscles by HPLC with fluorescence detection and confirmation using MS.
A sensitive and selective LC-FLD method was described for determination of closantel and rafoxanide in bovine and ovine muscles. Bovine and ovine muscles were extracted with ACN and acetone mixture (80:20, v/v). After cleanup with Oasis MAX SPE cartridges, the sample was analyzed by LC-FLD using the control point approach. No false-negative result was observed at or below maximum residue limits and the false-positive rate was below 5%. Suspected positive sample was confirmed by LC-MS/MS. This method was suitable for screening of large batch of samples and hence considerably reduced the time and cost required for quantitation and confirmatory analyses.
Yeung
Hoi-sze
HS
Analytical and Advisory Services Division, Government Laboratory, Ho Man Tin, Kowloon, Hong Kong Special Administration Region, PR China.
Lee
Wai-on
WO
Wong
Yiu-tung
YT
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000737:chemistry*; D011888:Rafoxanide / Q000032:analysis*; D012458:Salicylanilides / Q000032:analysis*; D012756:Sheep; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence; D053719:Tandem Mass Spectrometry
D012458:Salicylanilides; D011888:Rafoxanide; C023342:closantel