title: Identification of TRPM7 channels in human intestinal interstitial cells of Cajal.
abstract: OBJECTIVE
  To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract.
  
  
  METHODS
  Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs).
  
  
  RESULTS
  The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue.
  
  
  CONCLUSIONS
  These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
authors.affiliation: School of Korean Medicine, Pusan National University, Yangsan 626-870, South Korea.
authors.forename: Byung Joo
authors.initials: BJ
authors.lastname: Kim
authors.forename: Kyu Joo
authors.initials: KJ
authors.lastname: Park
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Seok
authors.initials: S
authors.lastname: Choi
authors.forename: Jae Yeoul
authors.initials: JY
authors.lastname: Jun
authors.forename: In Youb
authors.initials: IY
authors.lastname: Chang
authors.forename: Ju-Hong
authors.initials: JH
authors.lastname: Jeon
authors.forename: Insuk
authors.initials: I
authors.lastname: So
authors.forename: Seon Jeong
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001896:Boron Compounds / Q000494:pharmacology; D005260:Female; D005439:Flufenamic Acid / Q000494:pharmacology; D005769:Gastrointestinal Motility / Q000502:physiology; D006801:Humans; D056885:Interstitial Cells of Cajal / Q000166:cytology / Q000378:metabolism*; D007422:Intestines / Q000166:cytology*; D007811:Lanthanum / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018408:Patch-Clamp Techniques; D017346:Protein Serine-Threonine Kinases; D050053:TRPM Cation Channels / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D001896:Boron Compounds; D050053:TRPM Cation Channels; D005439:Flufenamic Acid; D007811:Lanthanum; C109986:2-aminoethoxydiphenyl borate; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human