title: HBsAg-vectored vaccines simultaneously deliver CTL responses to protective epitopes from multiple viral pathogens.
abstract: We have previously demonstrated that the potent immunogenicity of hepatitis B surface antigen (HBsAg) may be exploited to deliver foreign antigens for cytotoxic T-lymphocyte (CTL) induction. Here we demonstrate that a single low-dose immunization with rHBsAg DNA is sufficient to prime for CTL responses against encoded foreign epitope and that the responses may be recalled many months after immunization. We show that simultaneous disease-protective CTL responses restricted through a diversity of MHC class I haplotypes are elicited by recombinant (r) HBsAg DNA containing multiple viral epitopes appended as a C'-terminal polyepitope or encoded individually within the HBsAg polypeptide. CTL responses delivered by rHBsAg DNA were elicited in the presence of HBsAg-directed antibody. These studies vindicate the use of HBsAg as a powerful vector to deliver CTL responses to foreign antigen and have implications for a multidisease vaccine applicable to an MHC-polymorphic population.
authors.affiliation: Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Clinical Medical Virology Centre, University of Queensland, Brisbane, Australia.Shaanxi, Yangling, China. Chendekun163@163.com
authors.forename: Dekun
authors.initials: D
authors.lastname: Chen
authors.forename: Kristy
authors.initials: K
authors.lastname: Edgtton
authors.forename: Allan
authors.initials: A
authors.lastname: Gould
authors.forename: Huayang
authors.initials: H
authors.lastname: Guo
authors.forename: Michael
authors.initials: M
authors.lastname: Mather
authors.forename: Oscar
authors.initials: O
authors.lastname: Haigh
authors.forename: Melanie
authors.initials: M
authors.lastname: Cochrane
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Kattenbelt
authors.forename: Scott
authors.initials: S
authors.lastname: Thomson
authors.forename: Robert
authors.initials: R
authors.lastname: Tindle
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology; D004274:DNA, Recombinant; D004279:DNA, Viral; D015828:Eye Infections, Viral; D005260:Female; D006514:Hepatitis B Surface Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000502:physiology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D014614:Vaccines, Synthetic; D014765:Viral Vaccines