title: Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles.
abstract: The realization of biomolecular detection assays for diagnostic purposes is technologically very challenging because such tests demand full integration for ease of use and need to deliver a high analytical performance with cost-effective use of materials. In this article an optomagnetic immunoassay technology is described based on nanoparticles that are magnetically actuated and optically detected in a stationary sample fluid. The dynamic control of nanoparticles by magnetic fields impacts the key immunoassay process steps, giving unprecedented speed, assay control and seamless integration of the total test. The optical detection yields sensitive and multiplexed assays in a low-cost disposable cartridge. We demonstrate that the optomagnetic technology enables high-sensitivity one-step assays in blood serum/plasma and whole saliva. Drugs of abuse are detected at sub-nanogram per millilitre levels in a total assay time of 1 min, and the cardiac marker troponin I is detected at sub-picomole per litre concentrations in a few minutes. The optomagnetic technology is fundamentally suited for high-performance integrated testing and is expected to open a new paradigm in biosensing.
authors.affiliation: Philips Corporate Technologies, High Tech Campus, 5656 AE Eindhoven, The Netherlands.
authors.forename: D M
authors.initials: DM
authors.lastname: Bruls
authors.forename: T H
authors.initials: TH
authors.lastname: Evers
authors.forename: J A H
authors.initials: JA
authors.lastname: Kahlman
authors.forename: P J W
authors.initials: PJ
authors.lastname: van Lankvelt
authors.forename: M
authors.initials: M
authors.lastname: Ovsyanko
authors.forename: E G M
authors.initials: EG
authors.lastname: Pelssers
authors.forename: J J H B
authors.initials: JJ
authors.lastname: Schleipen
authors.forename: F K
authors.initials: FK
authors.lastname: de Theije
authors.forename: C A
authors.initials: CA
authors.lastname: Verschuren
authors.forename: T
authors.initials: T
authors.lastname: van der Wijk
authors.forename: J B A
authors.initials: JB
authors.lastname: van Zon
authors.forename: W U
authors.initials: WU
authors.lastname: Dittmer
authors.forename: A H J
authors.initials: AH
authors.lastname: Immink
authors.forename: J H
authors.initials: JH
authors.lastname: Nieuwenhuis
authors.forename: M W J
authors.initials: MW
authors.lastname: Prins
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D013287:Illicit Drugs / Q000032:analysis / Q000378:metabolism; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles; D055620:Optical Phenomena; D012463:Saliva / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors; D019210:Troponin I / Q000097:blood
chemical_list: D013287:Illicit Drugs; D019210:Troponin I; D012710:Serum Albumin, Bovine