title: Enhanced neurogenesis in the olfactory bulb in adult mice after injury induced by acute treatment with trimethyltin.
abstract: In adults, the subventricular zone is known to contain undifferentiated neural progenitor cells that proliferate and generate the olfactory bulb (OB) interneurons throughout life. We earlier showed that trimethyltin (TMT) causes neuronal damage in the granular cell layer of the OB in adult mice. In the current study, we examined neurogenesis in the OB in adult mice after injury induced by acute treatment with TMT. On day 2 post-TMT treatment, enhanced incorporation of 5-bromo-2'-deoxyuridine (BrdU) was seen in the granular cell layer of the OB. Many of the BrdU-labeled cells were undifferentiated cells on day 2 post-treatment. On day 30 post-TMT treatment, BrdU-labeled neuronal cells were dramatically increased in number in the granular cell layer of the OB. However, TMT treatment was ineffective in affecting the migration of BrdU-labeled cells from the subventricular zone to the OB. The results of a neurosphere assay revealed that the number of neurospheres derived from the OB was significantly increased on day 2 post-TMT treatment. The neurosphere-forming neural progenitor cells derived from the OB of TMT-treated animals were capable of differentiating into neuronal cells as well as into astrocytes. Taken together, our data suggest that the OB has the ability to undergo enhanced neurogenesis following TMT-induced neuronal injury in adult mice.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University Hirakata, Osaka, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Yoneyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawada
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Ogita
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000375:Aging; D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001921:Brain / Q000503:physiopathology; D001973:Bromodeoxyuridine; D002452:Cell Count; D002465:Cell Movement; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009498:Neurotoxins / Q000633:toxicity; D009830:Olfactory Bulb / Q000293:injuries* / Q000503:physiopathology*; D055153:Stem Cell Niche / Q000503:physiopathology*; D013997:Time Factors; D014298:Trimethyltin Compounds / Q000633:toxicity
chemical_list: D009498:Neurotoxins; D014298:Trimethyltin Compounds; C046488:trimethyltin; D001973:Bromodeoxyuridine