Potential biomarkers of muscle injury after eccentric exercise.
Proteomics was utilized to identify novel potential plasma biomarkers of exercise-induced muscle injury. Muscle injury was induced in nine human volunteers by eccentric upper extremity exercise. Liquid chromatography-mass spectrometry identified 30 peptides derived from nine proteins which showed significant change in abundance post-exercise. Four of these proteins, haemoglobin alpha chain, haemoglobin beta chain, alpha1-antichymotrypsin (ACT) and plasma C-1 protease inhibitor (C1 Inh), met the criterion for inclusion based on changes in at least two distinct peptides. ACT and C1 Inh peptides peaked earlier post-exercise than creatine kinase, and thus appear to provide new information on muscle response to injury.
Sietsema
Kathy E
KE
Harbor-UCLA Medical Center, Torrance, CA, USA.
Meng
Fanyu
F
Yates
Nathan A
NA
Hendrickson
Ronald C
RC
Liaw
Andy
A
Song
Qinghua
Q
Brass
Eric P
EP
Ulrich
Roger G
RG
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D015331:Cohort Studies; D050718:Complement C1 Inhibitor Protein / Q000096:biosynthesis; D003402:Creatine Kinase / Q000378:metabolism; D015444:Exercise; D005260:Female; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D009132:Muscles / Q000293:injuries*; D010455:Peptides / Q000097:blood / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D000514:alpha 1-Antichymotrypsin / Q000097:blood
D015415:Biomarkers; D050718:Complement C1 Inhibitor Protein; D006454:Hemoglobins; D010455:Peptides; D000514:alpha 1-Antichymotrypsin; C020622:hemoglobin B; D003402:Creatine Kinase