title: Blockade of vascular angiogenesis by Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujuba.
abstract: The matrix metalloproteinases (MMP) play an important role in tumor invasion, angiogenesis and inflammatory tissue destruction. Increased expression of MMP was observed in benign tissue hyperplasia and in atherosclerotic lesions. Invasive cancer cells utilize MMP to degrade the extracellular matrix and vascular basement membrane during metastasis, where MMP-2 has been implicated in the development and dissemination of malignancies. The present study attempted to examine the antiangiogenic activity of the medicinal herbs of Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujube (tAgR and tZj) with respect to MMP-2 production and endothelial motility in phorbol 12-myristate 13-acetate (PMA)- or VEGF-exposed human umbilical vein endothelial cells (HUVEC). Nontoxic tAgR and tZj substantially suppressed PMA-induced MMP-2 secretion. In addition, 25 microg/mL tAgR and tZj prevented vascular endothelial growth factor-stimulated endothelial cell transmigration and tube formation. The results reveal that tAgR and tZj dampened endothelial MMP-2 production leading to endothelial transmigration and tube formation. tAgR and tZj-mediated inhibition of endothelial MMP may boost a therapeutic efficacy during vascular angiogenesis.
authors.affiliation: Department of Food and Nutrition and Korean Institute of Nutrition, Hallym University, 39 Hallymdaehak-gil, Chuncheon, Kangwon 200-702, Korea.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Kang
authors.forename: Jung-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Bae
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Dong Shoo
authors.initials: DS
authors.lastname: Kim
authors.forename: Jung-Lye
authors.initials: JL
authors.lastname: Kim
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Shin
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: You
authors.forename: Hyoung-Sook
authors.initials: HS
authors.lastname: Park
authors.forename: Geun Eog
authors.initials: GE
authors.lastname: Ji
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Kang