O-glycosylation expression in fibroadenoma.
Fibroadenomas are human benign breast tumors characterized by proliferation of epithelial and stroma cells of the terminal ductal unit. Expression of O-glycans seems to contribute to the proliferation and transformation events. With this in mind, we evaluated the expression of glycans in fibroadenoma tissue through immunohistochemistry with antibodies against mucin epitopes (Anti CA15-3 and MUC1), as well as with lectins specific for glycans linked to proteins or lipids, and we compared findings with healthy breast specimens. Our results show positive expression of CA15-3 and MUC1 in fibroadenoma tissue, mainly in duct and stroma cells, whereas, in normal samples, staining was observed in duct cells. The lectin from Glycine max recognized equally well duct and stroma cells; this was the only lectin showing co-localization with anti-CA15-3 in healthy and tumor tissues. Dolichos biflorus, Artocarpus integrifolia, and Griffonia simplicifolia lectins recognized duct cells in control healthy tissues as well as in fibroadenoma tissue. The lectin from Amaranthus leucocarpus recognized only duct cells in control samples, whereas, in fibroadenoma tissue, it recognized duct and some stromal cells, suggesting that O-glycans-type mucin linked to proteins and mucin participate in the development of fibroadenomas.
Gallegos
Belem
B
Centro de Investigaciones en Ciencias Medicas y Biológicas, Facultad de Medicina, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México.
Pérez-Campos
Eduardo
E
Martinez
Ruth
R
Leyva
Paulina
P
Martinez
Margarito
M
Hernández
Rubi
R
Pina
Socorro
S
Hernández
Claudia
C
Zenteno
Edgar
E
Hernández
Pedro
P
D000906:Antibodies / Q000276:immunology; D031622:Artocarpus / Q000737:chemistry; D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000378:metabolism*; D002240:Carbohydrate Sequence; D031294:Dolichos / Q000737:chemistry; D005260:Female; D018226:Fibroadenoma / Q000378:metabolism*; D006031:Glycosylation; D031295:Griffonia / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D016612:Paraffin Embedding; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry
D000906:Antibodies; D037102:Lectins; D018396:Mucin-1; D010940:Plant Proteins; D011134:Polysaccharides