title: Immunolocalization and mRNA expression of bone morphogenetic protein-6 in human clear cell renal carcinoma.
abstract: Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-beta superfamily of proteins. Dysregulation of BMP signaling has been suggested in the carcinogenesis of different organs. We determined BMP-6 mRNA and protein expression in localized human clear cell renal carcinoma (CCRC), obtained from 20 patients who underwent nephrectomy, by the real-time polymerase chain reaction and immunohistochemistry. 15/20 patients exhibited higher BMP-6 mRNA expression in malignant than in healthy renal tissue relative to the PBGD expression (p < 0.05). Immunostaining intensity for BMP-6 in healthy renal tissue ranged from 0 to 2 (average 0.9), as well as in renal clear cell carcinoma (average 1.1). Seven of 20 (35%) healthy tissue samples failed to stain with BMP-6 antibody, compared to 2/20 (10%) tumor samples (p < 0.05). BMP-6 immunostaining was positive in 18/20 CCRC samples. Staining was localized in the cytoplasm and/or membrane of malignant cells. Malignant tissue had significantly higher BMP-6 mRNA expression than healthy tissue. There was no significant correlation between BMP-6 mRNA and protein expression with disease presentation, disease progression and patients' characteristics. Long-term follow-up of our patients is needed to determine the possible role of increased expression of BMP-6 in CCRC.
authors.affiliation: Department of Dialysis, University Hospital Center Zagreb, Zagreb, Croatia. nina_basic@net.hr
authors.forename: Nikolina
authors.initials: N
authors.lastname: Basic-Jukic
authors.forename: Margareta
authors.initials: M
authors.lastname: Radic-Antolic
authors.forename: Tvrtko
authors.initials: T
authors.lastname: Hudolin
authors.forename: Marijana
authors.initials: M
authors.lastname: Coric
authors.forename: Renata
authors.initials: R
authors.lastname: Zadro
authors.forename: Josip
authors.initials: J
authors.lastname: Pasini
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Kastelan
authors.forename: Petar
authors.initials: P
authors.lastname: Kes
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D055418:Bone Morphogenetic Protein 6; D012333:RNA, Messenger