In vitro antigen-specific cytotoxic T cell response against esophageal carcinoma cells induced by HPV18E7-transfected dendritic cells.
Human Papillomavirus (HPV)-associated esophageal carcinoma (EC) is a high incidence tumor worldwide. Dendritic cell (DC)-based tumor vaccine is considered an alternative therapy to treat EC. Here we developed a DC-based vaccine by transfecting cord blood CD34+ stem cell-derived DC with HPV18E7 gene, observed its biological characteristics and the antigen-specific T-cell cytotoxicity on EC cells induced by HPV18E7-DC in vitro. Our results showed that 1) HPV18E7 gene transfer did not change the typical morphology of mature DC, 2) the representative phenotypes of mature DC (CD80, CD86, and CD83) were highly expressed in HPV18E7- DC (81.6%, 80.5%, and 86.6%, respectively), 3) the expression level of 18E7 protein in HPV18E7-DC was 47.5%, and 4) the specific cytotoxicity against EC cells was significantly higher than that in controls (p<0.01). This study indicates the possibility of a DC-based immunotherapy in HPV-associated EC.
Wu
Lin
L
The Second Affiliated Hospital of Shan Tou University Medical College, Shan Tou, GuangDong, China.
Yang
Wei
W
Chen
Lin-Xin
LX
Chen
Shen-Ren
SR
Zhang
Jin-Kun
JK
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D019496:Cancer Vaccines; D002277:Carcinoma / Q000235:genetics / Q000276:immunology* / Q000821:virology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D053686:Fetal Stem Cells / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D000097802:CD83 Antigen
D015703:Antigens, CD; D018952:Antigens, CD34; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019496:Cancer Vaccines; D004268:DNA-Binding Proteins; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D009856:Oncogene Proteins, Viral; D000097802:CD83 Antigen; C497964:CD86 protein, human; C052633:E7 protein, Human papillomavirus type 18