title: Differential accumulation of volatile terpene and terpene synthase mRNAs during lavender (Lavandula angustifolia and L. x intermedia) inflorescence development.
abstract: Despite the commercial importance of Lavandula angustifolia Mill. and L. x intermedia Emeric ex Loisel floral essential oils (EOs), no information is currently available on potential changes in individual volatile organic compound (VOC) content during inflorescence development. Calyces were found to be the main sites of VOC accumulation. The 20 most abundant VOCs could be separated into three sub-groups according to their patterns of change in concentration The three groups of VOCs sequentially dominated the global scent bouquet of inflorescences, the transition between the first and second groups occurring around the opening of the first flower of the inflorescence and the one between the second and third groups at the start of seed set. Changes in calyx VOC accumulation were linked to the developmental stage of individual flowers. Leaves accumulated a smaller number of VOCs which were a subset of those seen in preflowering inflorescences. Their nature and content remained constant during the growing season. Quantitative real time polymerase chain reaction assessments of the expression of two terpene synthase (TPS) genes, LaLIMS and LaLINS, revealed similar trends between their patterns of expression and those of their VOC products. Molecular and chemical analyses suggest that changes in TPS expression occur during lavender inflorescence development and lead to changes in EO composition. Both molecular data and terpene analysis support the findings that changes in biosynthesis of terpene occurred during inflorescence development.
authors.affiliation: Université de Lyon, F-69003, Lyon, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guitton
authors.forename: Florence
authors.initials: F
authors.lastname: Nicolè
authors.forename: Sandrine
authors.initials: S
authors.lastname: Moja
authors.forename: Nadine
authors.initials: N
authors.lastname: Valot
authors.forename: Sylvain
authors.initials: S
authors.lastname: Legrand
authors.forename: Frédéric
authors.initials: F
authors.lastname: Jullien
authors.forename: Laurent
authors.initials: L
authors.lastname: Legendre
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D056626:Inflorescence / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D027523:Lavandula / Q000201:enzymology / Q000235:genetics*; D008855:Microscopy, Electron, Scanning; D009822:Oils, Volatile / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010938:Plant Oils / Q000032:analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D013729:Terpenes; D055549:Volatile Organic Compounds; D006836:Hydro-Lyases; C092055:S-linalool synthase; D019753:Intramolecular Lyases; C040265:pinene cyclase I