N-methylated cyclic enkephalin analogues retain high opioid receptor binding affinity.
In an effort to improve the bioavailability of the non-selective, cyclic enkephalin analogues H-Dmt-c[d-Cys-Gly-Phe-d(or L)-Cys]NH(2) (Dmt = 2',6'-dimethyltyrosine), analogues N-methylated at the Phe(4) and/or Cys(5) residue were synthesized. In comparison with the non-methylated parent peptides, all mono- and N-di-methylated analogues in general retained high binding affinities at all three opioid receptors and high opioid agonist potencies in functional opioid activity assays. The results indicate that the progressive conformational restriction in these compounds upon mono- and di-N-methylation did not significantly affect the in vitro opioid activity profile. A low-energy conformer identified for the conformationally most restricted analogue of the series, H-Dmt-c[D-Cys-Gly-Phe(NMe)-L-Cys(NMe)]NH(2) (6), showed good spatial overlap of the essential pharmacophoric moieties with those in the proposed mu receptor-bound conformation of the mu-selective opioid peptide JOM-6 [H-Tyr-c(S-Et-S)[D-Cys-Phe-D-Pen]NH(2)] (Pen = penicillamine) [Mosberg M.I. and Fowler C.B. (2002) J Peptide Res; 60:329-335], in agreement with the moderate mu selectivity determined for this compound. An analogue of 6 containing (2S)-2-methyl-3-(2,6-dimethyl-4-hydroxyphenyl)propanoic acid [(2S)-Mdp] in place of Dmt(1) was an opioid antagonist with quite high opioid receptor binding affinities and can be expected to show improved bioavailability because of its further increased lipophilicity and reduced hydrogen-bonding capacity.
Weltrowska
Grazyna
G
Clinical Research Institute of Montreal, Quebec, Canada.
Berezowska
Irena
I
Lemieux
Carole
C
Chung
Nga N
NN
Wilkes
Brian C
BC
Schiller
Peter W
PW
D000595:Amino Acid Sequence; D000818:Animals; D003198:Computer Simulation; D004745:Enkephalins / Q000138:chemical synthesis / Q000737:chemistry*; D006168:Guinea Pigs; D008745:Methylation; D051379:Mice; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000737:chemistry*; D017473:Receptors, Opioid, kappa / Q000819:agonists / Q000737:chemistry*; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000737:chemistry*
D004745:Enkephalins; D010456:Peptides, Cyclic; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu