[Evaluation of serum levels of SOD and MDA in patients with Leber's hereditary optic neuropathy carrying the mitochondrial DNA G11778A mutation].
OBJECTIVE
To determine the serum levels of total superoxide dismutase (SOD) activity and malondialdehyde (MDA), and to evaluate the oxidant-antioxidant status in patients with Leber's hereditary optic neuropathy (LHON) carrying the mitochondrial G11778A mutation.


METHODS
Nineteen patients and 12 carriers from three Chinese G11778A LHON families were enrolled in this study, and 30 age-matched healthy volunteers were recruited as normal controls. The serum levels of total SOD activity and MDA in all subjects were measured by xanthine oxidase test and thiobarbituric acid technique, respectively.


RESULTS
The serum level of total SOD activity in LHON patients was significantly less than that in carriers and normal controls (q = 7.085 and 8.351, respectively, both P < 0.05), however, there was no significant difference between the carriers and normal controls (q = 0.269, P > 0.05). The serum level of MDA in patients and carriers was significantly higher than that in normal controls (q = 9.069 and 4.748, respectively, both P < 0.05), and it was also significantly higher in patients than that in carriers (q = 3.618, P < 0.05).


CONCLUSIONS
Antioxidant capacity decreased significantly in patients with LHON, which indicates that the onset of LHON was related with the oxidation-antioxidation imbalance.
Liu
Zhe
Z
School of Medicine, Zhejiang University, Hangzhou 310009, China.
Sun
Chuan-bin
CB
Tong
Yi
Y
Qu
Jia
J
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009154:Mutation; D029242:Optic Atrophy, Hereditary, Leber / Q000097:blood* / Q000235:genetics*; D013482:Superoxide Dismutase / Q000097:blood*; D055815:Young Adult
D004272:DNA, Mitochondrial; D008315:Malondialdehyde; D013482:Superoxide Dismutase