title: Molecular characterization and bioactivity of a CXCL13 chemokine in large yellow croaker Pseudosciaena crocea.
abstract: A CXCL13-like chemokine cDNA was isolated from large yellow croaker (Pseudosciaena crocea) by expressed sequence tag (EST) analysis (LycCXCL13). The full-length cDNA of LycCXCL13 is 796 nucleotides (nt) encoding a protein of 97 amino acids (aa), with a putative molecular weight of 10.7 kDa. The deduced LycCXCL13 contains a 24-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known CXC chemokines (C(25), C(27), C(52) and C(68)). It shares 35, 36 and 39% aa sequence identities to green puffer CXCL13-like, Atlantic salmon CXCL13 and Japanese flounder CXCL13 chemokines, and 24-29% identities to CXCL13 chemokines in mammals, respectively. Phylogenetic analysis showed that LycCXCL13 is more closely related to the CXCL13 subgroup than to any other CXC chemokine subgroups. LycCXCL13 gene was constitutively expressed in all tissues examined, except for intestine. Upon induction with poly(I:C) or inactivated trivalent bacterial vaccine, LycCXCL13 gene expression was significantly up-regulated in spleen, head kidney, heart and gills at 24 h post-injection. Real-time PCR results showed that LycCXCL13 gene expression reached peak level in spleen and head kidney at 12 h after induction by poly(I:C), while its expression increased to the highest level in head kidney at 24 h or in spleen at 48 h by bacterial vaccine. Recombinant LycCXCL13 protein produced in E. coli BL21 exhibited obvious chemotaxis to the peripheral blood leucocytes (PBLs) from large yellow croaker. These results suggest that LycCXCL13 may be involved in inflammatory responses as well as homeostatic processes in large yellow croaker.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tian
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jingqun
authors.initials: J
authors.lastname: Ao
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D001483:Base Sequence; D054382:Chemokine CXCL13 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D007369:Interferon Inducers / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001428:Bacterial Vaccines; D054382:Chemokine CXCL13; D007369:Interferon Inducers; D011994:Recombinant Proteins; D011070:Poly I-C