Evolving protein stability through genetic selection.
In this issue of Molecular Cell, Foit et al. (2009) probe cellular protein folding using a split beta-lactamase approach for evolving protein stability in the absence of any requirement for function.
Wiseman
R Luke
RL
Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. wiseman@scripps.edu
Kelly
Jeffery W
JW
D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D017510:Protein Folding; D055550:Protein Stability; D012641:Selection, Genetic
D002352:Carrier Proteins; C509253:E colicin-binding immunity protein Im7, E coli; D029968:Escherichia coli Proteins