title: Wrestling with native chemical ligation.
abstract: An improved method for the semisynthesis of a potassium channel involving native chemical ligation allows the introduction of short sequences containing non-canonical amino acids at any position within the polypeptide chain. The work enhances the technology available for a range of fundamental investigations of membrane proteins and for applications of membrane channels and pores in biotechnology.
authors.affiliation: Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom. hagan.bayley@chem.ox.ac.uk
authors.forename: Hagan
authors.initials: H
authors.lastname: Bayley
authors.forename: Stephen
authors.initials: S
authors.lastname: Cheley
authors.forename: Leon
authors.initials: L
authors.lastname: Harrington
authors.forename: Ruhma
authors.initials: R
authors.lastname: Syeda
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D001426:Bacterial Proteins / Q000138:chemical synthesis / Q000737:chemistry; D008958:Models, Molecular; D015221:Potassium Channels / Q000138:chemical synthesis* / Q000737:chemistry; D011487:Protein Conformation; D051381:Rats
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D015221:Potassium Channels; C107790:prokaryotic potassium channel