Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates.
A straightforward method for the rapid detection of the presence of glutathione S-transferase (GST)-tagged proteins from sample solutions using glutathione (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as the detection probes by simple observation with the naked eye was proposed in this study.
Chen
Cheng-Tai
CT
Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
Chen
Wei-Jen
WJ
Liu
Chao-Zong
CZ
Chang
Ling-Ya
LY
Chen
Yu-Chie
YC
D003030:Coagulase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; D006046:Gold; D005340:Fibrinogen; D005982:Glutathione Transferase; D005978:Glutathione