Investigation of porcine endogenous retrovirus in the conservation population of Ningxiang pig.
Porcine endogenous retrovirus (PERV) varies between pig breeds. Screening and analysis of PERV in putative pig breeds may provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. In this study, PERV was investigated among the conservation population of the Ningxiang pig. The result revealed that the genotype of PERV distribution was subtype A, 100%; subtype B, 100%; and subtype C, 100%. The env sequences of PERV-A and -B showed 11 clones detected by KpnI and MboI digestion, indicating that there existed multiple variants of PERV-A and -B in the Ningxiang pig. Reverse transcriptase polymerase chain reaction results showed that PERV had transcriptional activity in these individuals. In addition, PERV A/C recombinant was detected in most individuals of Ningxiang pig. Because PERV A/C recombinants increase the potential infectious risk, the breed may not be a proper donor for xenotransplantation.
Xing
X W
XW
Cell Transplantation and Gene Therapy Institute of Central South University, Changsha 410013, P. R. China.
Hawthorne
W J
WJ
Yi
S
S
Simond
D M
DM
Dong
Q
Q
Ye
B
B
Tong
Q J
QJ
Ye
Z
Z
Wang
W
W
D000818:Animals; D002681:China; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D020077:Endogenous Retroviruses / Q000235:genetics / Q000502:physiology*; D017988:Gammaretrovirus; D014644:Genetic Variation; D006801:Humans; D009926:Organ Preservation / Q000379:methods / Q000592:standards; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D013552:Swine / Q000097:blood / Q000235:genetics* / Q000821:virology; D014158:Transcription, Genetic; D014183:Transplantation, Heterologous / Q000639:trends
D017931:DNA Primers; D004272:DNA, Mitochondrial; D004279:DNA, Viral; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase