title: Mkl transcription cofactors regulate structural plasticity in hippocampal neurons.
abstract: Expressed throughout the central nervous system, the myocardin-related, megakaryoblastic acute leukemia 1 and 2 (Mkl1/2) are transcriptional cofactors that can be found tethered in the cytoplasm to monomeric actin but on synaptic activation translocate to the nucleus and associate with transcription factors such as serum response factor (SRF) to regulate expression of structural genes. This implies a potential role for Mkls in linking synaptic activity, through gene-expression control, to neuronal structural plasticity. Here, we present evidence that Mkls, particularly Mkl2, are powerful regulators of neuronal structure in vitro. Moreover, using the passive avoidance-conditioning paradigm, we identify learning-associated alterations of neuronal Mkl expression that appear to contribute to 2 phases of gene regulation during memory consolidation in the hippocampus. Gene regulation immediately after learning includes Egr2 and may be facilitated by downregulation of Mkls likely releasing ternary complex factor-regulated SRF activity. The second transcriptional phase occurs later at the 3-h postavoidance time point when Mkl accumulates in the nucleus of hippocampal neurons and there is enhanced transcription of Mkl-dependent structural genes that may contribute to the elaboration of new, memory-associated synapses known to appear over the subsequent 3-h period.
authors.affiliation: Applied Neurotherapeutics Research Group, UCD Schools of Biomolecular and Biomedical Science, UCD Conway Institute, University College Dublin, Dublin, Ireland. ruthger.righart@unige.ch
authors.forename: Niamh C
authors.initials: NC
authors.lastname: O'Sullivan
authors.forename: Mark
authors.initials: M
authors.lastname: Pickering
authors.forename: Danika
authors.initials: D
authors.lastname: Di Giacomo
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Loscher
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D016547:Kinesins / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D051767:Early Growth Response Protein 2; C497328:Egr2 protein, rat; D014157:Transcription Factors; C502895:Kif11 protein, rat; D016547:Kinesins