Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis.
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
Ramos
Carlos A N
CA
Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
Araújo
Flábio R
FR
Souza
Ingrid I F
II
Oliveira
Renato H M
RH
Elisei
Carina
C
Soares
Cleber O
CO
Sacco
Ana M S
AM
Rosinha
Grácia M S
GM
Alves
Leucio C
LC
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000097:blood*; D016793:Babesia bovis / Q000276:immunology* / Q000302:isolation & purification; D001404:Babesiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology; D007074:Immunoglobulin G / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0