Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS.
In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R(ct)) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.
Truong
Thi Ngoc Lien
TN
Hanoi University of Technology, Hanoi 8404, Viet Nam.
Tran
Dai Lam
DL
Vu
Thi Hong An
TH
Tran
Vinh Hoang
VH
Duong
Tuan Quang
TQ
Dinh
Quang Khieu
QK
Tsukahara
Toshifumi
T
Lee
Young Hoon
YH
Kim
Jong Seung
JS
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D017796:Caulimovirus / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017097:Electric Impedance; D004563:Electrochemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D030781:Organisms, Genetically Modified; D011108:Polymers / Q000737:chemistry*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011758:Pyrroles / Q000737:chemistry*; D011791:Quartz; D013025:Glycine max / Q000235:genetics; D013057:Spectrum Analysis; D013194:Staining and Labeling; D013997:Time Factors
D037742:Nanotubes, Carbon; D009838:Oligodeoxyribonucleotides; D011108:Polymers; D011758:Pyrroles; D011791:Quartz; C067635:polypyrrole; D004247:DNA