title: Designing and evaluation of sodium selenite nanoparticles in vitro to improve selenium absorption in ruminants.
abstract: Sodium selenite is used to prevent selenium deficiency known as nutritional muscular dystrophy or white muscle disease. In ruminants, selenium supplements are transformed partiality in insoluble form by ruminal microorganisms and its process decrease the selenium absorption in digestive gastrointestinal. However, the objective in this research was focused in encapsulated sodium selenite to be release into of a pH less than four, similarity to an intestinal environment. It was encapsulated by nanoprecipitation and emulsion-evaporation methods, within polymeric nanoparticles. The effect of these methods, polymer proportion (Eudragit RL and RS) and solvent (ethanol and acetone) on the physicochemical (drug entrapment, polidispersity index (PDI) and z potential) and morphological characteristics (particle morphology and particle size) were evaluated. Particle size from each nanoparticles, formulation ranged from 36.64 to 213.86 nm. Particle size, z potential and PDI increased (P <or= 0.01) when nanoprecipitation and ethanol were used. No significant differences (P > 0.05) were observed when different polymeric proportions were used. Selenium entrapment was 26% when emulsion-evaporation method was used and 78% with nanoprecipitation. Nanoparticles produced by nanoprecipitation were spherical and had a great variation in particle size; on the other hand, nanoparticles produced by emulsion-evaporation were spherical as well as amorphous and presented a homogeneous nanopartcicle size distribution. The release of selenium from nanoparticles was higher in acid pH (less than 4), this condition may represent a better availability of the mineral in the small intestine.
authors.affiliation: Colegio de Postgraduados, enlace ganadería, Montecillo Km 36.5 Carr., CP 56230, Texcoco, Mexico.
authors.forename: Atmir
authors.initials: A
authors.lastname: Romero-Pérez
authors.forename: Elizabeth
authors.initials: E
authors.lastname: García-García
authors.forename: Araceli
authors.initials: A
authors.lastname: Zavaleta-Mancera
authors.forename: J Efren
authors.initials: JE
authors.lastname: Ramírez-Bribiesca
authors.forename: Alma
authors.initials: A
authors.lastname: Revilla-Vázquez
authors.forename: Luz M
authors.initials: LM
authors.lastname: Hernández-Calva
authors.forename: Raquel
authors.initials: R
authors.lastname: López-Arellano
authors.forename: Rosy G
authors.initials: RG
authors.lastname: Cruz-Monterrosa
mesh_terms: D000096:Acetone / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions; D000431:Ethanol / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D012418:Ruminants; D012643:Selenium; D018038:Sodium Selenite / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D004655:Emulsions; D053769:Nanocapsules; D011108:Polymers; D000096:Acetone; C050528:Eudragit RS; D000431:Ethanol; C058665:Eudragit RL; D012643:Selenium; D018038:Sodium Selenite