title: Comparison of DiaSorin and Bio-Rad test kits for the detection of hepatitis B virus total core and surface antibodies on the Bio-Rad Evolis.
abstract: The new MONOLISA Bio-Rad kits were compared to DiaSorin kits for the detection of hepatitis B virus (HBV) total core (HBcTAb) and surface (HBsAb) antibodies on the Bio-Rad Evolis immunoanalyzer. The resolved sensitivities, specificities, positive and negative predictive values, and overall accuracy were 91%, 100%, 100%, 96%, and 97%, respectively, for HBcTAb and 99%, 95%, 96%, 99%, and 97%, respectively, for HBsAb. Whereas accuracy and reagent cost were comparable between the kits, Bio-Rad kits required less specimen volume and less instrument processing time to results than the DiaSorin kits.
authors.affiliation: Department of Pathology, Creighton University Medical Center, Omaha, NE 68131, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cavalieri
authors.forename: Sharon
authors.initials: S
authors.lastname: Hrabovsky
authors.forename: Trichel
authors.initials: T
authors.lastname: Jorgensen
mesh_terms: D005189:False Positive Reactions; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic