Vascular endothelial growth factor concentrations from platelets correlate with tumor angiogenesis and grading in a spontaneous canine non-Hodgkin lymphoma model.
Published data strongly suggest that tumor progression and malignancy are associated with increased angiogenesis. However, no data have been published concerning the relationship between microvascular density (MVD), tumor cytosol, and blood vascular endothelial growth factor (VEGF) concentrations in canine non-Hodgkin lymphoma (C-NHL), a neoplasm that shares biological and clinical characteristics with human NHL. We have evaluated MVD and tumor cytosol, serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) VEGF concentration in a series of 63 B-cells C-NHL by means of immunohistochemistry and enzyme-linked immuno-sorbent assay (ELISA) detection of VEGF. We found that MVD, VEGF from cytosol, and VEGF from P-APR are significantly correlated (p ranging from 0.001 to 0.003) and that these parameters paralleled with the malignancy degree of NHL. Accordingly, spontaneous C-NHL seems to be an interesting model to study the role of angiogenesis as interspecies pathway of tumor malignancy and we suggest that VEGF from P-APR might be a novel useful circulating bio-marker of tumor angiogenesis.
Zizzo
Nicola
N
Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy.
Patruno
Rosa
R
Zito
Francesco Alfredo
FA
Di Summa
Aldo
A
Tinelli
Antonella
A
Troilo
Sante
S
Misino
Andrea
A
Ruggieri
Eustachio
E
Goffredo
Veronica
V
Gadaleta
Cosmo Damiano
CD
Ranieri
Girolamo
G
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000662:veterinary*; D055806:Microvessels / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000662:veterinary*; D053657:Platelet-Rich Plasma / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood* / Q000378:metabolism
D042461:Vascular Endothelial Growth Factor A