Assessment of the reproducibility of random hexapeptide peptide library-based protein normalization.
The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.
Dwivedi
Ravi C
RC
Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg MB, Canada.
Krokhin
Oleg V
OV
Cortens
John P
JP
Wilkins
John A
JA
D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009842:Oligopeptides / Q000737:chemistry*; D019151:Peptide Library; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D009842:Oligopeptides; D019151:Peptide Library; D011506:Proteins; D020543:Proteome