title: Electrophoretic separation of conformational states of alpha-chymotrypsinogen A at high pressures.
abstract: When reversible denaturation of chymotrypsinogen is produced at elevated pressures and low temperatures, the transition has been observed to occur very slowly. Electrophoretic separation of the transition mixture at high pressures reveals the presence of two distinct molecular species. This evidence, in conjunction with previously reported spectroscopic data, suggests that a simple two-state model provides a useful description of the transition phenomena at high pressures.
authors.affiliation: Jefferson Physical Research Laboratory, Harvard University, Cambridge, MA 02138, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Hawley
mesh_terms: D002021:Buffers; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002919:Chymotrypsinogen / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011312:Pressure; D011487:Protein Conformation; D011489:Protein Denaturation; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D013816:Thermodynamics
chemical_list: D002021:Buffers; D002919:Chymotrypsinogen