Gelsolin is required for macrophage recruitment during remyelination of the peripheral nervous system.
Reorganization of the actin cytoskeleton is necessary for Schwann cell proliferation, migration and for the morphological changes associated with sorting, ensheathing and myelination of axons. Such reorganization requires regulated severing and depolymerization of actin filaments. Gelsolin is an actin filament severing protein expressed in many cell types including Schwann cells. Using Gelsolin knockout mice, we investigated the role of this protein in the myelination and remyelination of the peripheral nervous system. Our results show that although gelsolin is not necessary for developmental myelination, it is required for timely remyelination of the sciatic nerve following crush injury. Gelsolin is necessary for macrophage motility in culture, and its absence is likely to impair the recruitment of macrophages to the injury site.
Gonçalves
Ana Filipa
AF
Institute of Cell Biology, Department of Biology, ETH Zurich, Zurich, Switzerland.
Dias
Nuno G
NG
Moransard
Martijn
M
Correia
Ricardo
R
Pereira
Jorge A
JA
Witke
Walter
W
Suter
Ueli
U
Relvas
João B
JB
D000367:Age Factors; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D018260:Gelsolin / Q000172:deficiency / Q000378:metabolism*; D020287:In Situ Nick-End Labeling / Q000379:methods; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D000075385:Netrins; D012583:Schwann Cells / Q000502:physiology* / Q000648:ultrastructure; D020426:Sciatic Neuropathy / Q000473:pathology* / Q000503:physiopathology*; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D000943:Antigens, Differentiation; D018260:Gelsolin; D009419:Nerve Tissue Proteins; D000075385:Netrins; C456263:Ntng2 protein, mouse; C036763:monocyte-macrophage differentiation antigen; D020830:rac1 GTP-Binding Protein; D020741:rho GTP-Binding Proteins