title: Efficient protein renaturation using tunable hemifluorinated anionic surfactants as additives.
abstract: We show that hemifluorinated anionic surfactants are effective as renaturing additives for bovine carbonic anhydrase (CAB) below their critical micelle concentration (CMC) providing high refolding yields for thermally and chemically denatured protein.
authors.affiliation: Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA.
authors.forename: Rajni
authors.initials: R
authors.lastname: Singh
authors.forename: Robert A
authors.initials: RA
authors.lastname: Flowers
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry*; D002417:Cattle; D002942:Circular Dichroism; D054879:Halogenation; D008823:Micelles; D017510:Protein Folding; D020673:Protein Renaturation; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature
chemical_list: D000838:Anions; D008823:Micelles; D013501:Surface-Active Agents; D002256:Carbonic Anhydrases