title: Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120.
abstract: We evaluated the potential of a quartz crystal microbalance with dissipation monitoring (QCM-D) to provide a sensitive, label-free method for detecting the conformational rearrangement of glycoprotein gp120 upon binding to different ligands. This glycoprotein is normally found on the envelope of the HIV-1 virus and is involved in viral entry into host cells. It was immobilized on the surface of the sensing element of the QCM-D and was exposed to individual solutions of several different small-molecule inhibitors as well as to a solution of a soluble form of the host cell receptor to which gp120 binds. Instrument responses to ligand-triggered changes were in qualitative agreement with conformational changes as suggested by other biophysical methods.
authors.affiliation: Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA.
authors.forename: Hyun-Su
authors.initials: HS
authors.lastname: Lee
authors.forename: Mark
authors.initials: M
authors.lastname: Contarino
authors.forename: M
authors.initials: M
authors.lastname: Umashankara
authors.forename: Arne
authors.initials: A
authors.lastname: Schön
authors.forename: Ernesto
authors.initials: E
authors.lastname: Freire
authors.forename: Amos B
authors.initials: AB
authors.lastname: Smith
authors.forename: Irwin M
authors.initials: IM
authors.lastname: Chaiken
authors.forename: Lynn S
authors.initials: LS
authors.lastname: Penn
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D015699:HIV Envelope Protein gp120 / Q000032:analysis* / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D008024:Ligands; D011485:Protein Binding; D011487:Protein Conformation; D011791:Quartz
chemical_list: D015699:HIV Envelope Protein gp120; D008024:Ligands; C514793:gp120 protein, Human immunodeficiency virus 1; D011791:Quartz