Identification and characterization of the type 2C protein phosphatase Ptc4p in the human fungal pathogen Candida albicans.
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ions. There are seven PP2C genes, named from PTC1 to PTC7, in Saccharomyces cerevisiae. In the current study we identified the CaPTC4 gene in Candida albicans and demonstrated that the CaPtc4p protein is a typical PP2C enzyme, which is highly conserved in fungal species. Deletion of CaPTC4 renders Candida cells sensitive to sodium and potassium ions as well as to antifungal azole drugs. In addition, we have shown that CaPtc4p is localized in the mitochondrion, suggesting that CaPtc4p is likely to be involved in the regulation of a mitochondrial function related to ion homeostasis.
Zhao
Jingwen
J
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
Sun
Xin
X
Fang
Jiaping
J
Liu
Wei
W
Feng
Chen
C
Jiang
Linghuo
L
D000935:Antifungal Agents / Q000494:pharmacology; D001393:Azoles / Q000494:pharmacology; D002176:Candida albicans / Q000201:enzymology*; D017124:Conserved Sequence; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D008928:Mitochondria / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism / Q000633:toxicity; D000071636:Protein Phosphatase 2C; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism / Q000633:toxicity
D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D012964:Sodium; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D011188:Potassium