title: Identification and characterization of small sub-genomic RNAs in dengue 1-4 virus-infected cell cultures and tissues.
abstract: Dengue virus (DV) contains a single-stranded, positive-sense RNA genome, and the 3' non-coding regions (NCRs) have been demonstrated to play crucial roles in viral RNA replication and translation initiation. In this study, we report the presence of a species of small sub-genomic RNA (sgRNA) derived from the 3' NCR in DV-infected cell cultures and animal tissues. Further experiments demonstrate that these sgRNAs range from 390 to 430 nt in length and that they accumulate together with viral genomic RNA during replication. Sequence analysis and secondary structure prediction indicate that the stem-loop structures within the 3' NCR are responsible for the production of DV sgRNAs. Unexpectedly, a unique DV isolate produced three species of sgRNAs, compared to only one in the other isolates. Collectively, the structural and biochemical properties of DV sgRNAs warrant further study of their biological functions during the viral life cycle and pathogenesis.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Yue
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Xuedong
authors.initials: X
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhenyou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Ede
authors.initials: E
authors.lastname: Qin
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D003716:Dengue Virus / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*
chemical_list: D012367:RNA, Viral