Allelopathy and allelopathic substance in the moss Rhynchostegium pallidifolium.
An aqueous methanol extract of the moss Rhynchostegium pallidifolium, which often forms large pure colonies on soils and rocks, inhibited the growth of cress (Lepidium sativum), alfalfa (Medicaga sativa), lettuce (Lepidium sativum), ryegrass (Lolium multiflorum), timothy (Phleum pratense) and Digitaria sanguinalis seedlings. Increasing the extract concentration increased the inhibition. These results suggest that R. pallidifolium may show allelopathic activity. The extract was purified and a putative compound causing this growth inhibitory effect was isolated. The chemical structure of the growth inhibitor was determined by MS, and (1)H and (13)C NMR spectral data as 3-hydroxy-beta-ionone. 3-Hydroxy-beta-ionone inhibited the shoot and root growth of cress seedlings at concentrations greater than 1 and 3 micromol/L, respectively. The doses required for 50% growth inhibition on the shoot and roots of cress seedlings were 16.3 and 14.9 micromol/L, respectively. The endogenous concentration of 3-hydroxy-beta-ionone in R. pallidifolium was 28.2 microg/g and the concentration of 3-hydroxy-beta-ionone in the growth medium of R. pallidifolium was 6.7 microg/g. These results suggest that 3-hydroxy-beta-ionone was likely secreted into the medium during the incubation of R. pallidifolium. In addition, 3-hydroxy-beta-ionone was found in the soil under the pure colonies of R. pallidifolium. Therefore, 3-hydroxy-beta-ionone may play an important role in the allelopathic activity of R. pallidifolium and may help competition with neighboring plants, resulting in the formation of pure colonies.
Kato-Noguchi
Hisashi
H
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan. hisashi@ag.kagawa-u.ac.jp
Seki
Takahiro
T
Shigemori
Hideyuki
H
D044002:Bryophyta / Q000737:chemistry*; D006131:Growth Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D031220:Lepidium sativum / Q000187:drug effects / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D045792:Norisoprenoids / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development
D006131:Growth Inhibitors; D045792:Norisoprenoids; D010936:Plant Extracts; C008157:beta-ionone