title: Molecular cloning and phylogenetic analysis of integrins alphavbeta1 and alphavbeta6 of one-humped camel (Camelus dromedarius).
abstract: Bactrian camels can relatively easily be infected with FMDV, but dromedary camels remain resistant even to high doses of the virus. To understand the different susceptibility between the two camel species from the standpoint of viral receptors, this work reports the sequences of the dromedary camel integrin cDNAs encoding alphavbeta1 and alphavbeta6 and compare them to those of other species, especially to Bactrian camels. The complete coding sequences for the dromedary camel alphav, beta1 and beta6 subunits were found to be 3147, 2397, and 2364 nucleotides in length, encoding 1048, 798, and 787 amino acids, respectively. The dromedary camel integrin alphav, beta1, and beta6 subunit shares common structural and functional elements with their counterparts from the other species. Phylogenetic trees showed that the dromedary camel alphav, beta1, and beta6 were clustered into the Artiodactyla group, together with those of Bactrian camel, pig, sheep, and cattle that are susceptible to FMDV infection. Compared with the Bactrian camel integrins, 4, 10, and 8 amino acid changes were found in the dromedary camel alphav, beta1, and beta6 subunits, respectively. This study will be of importance in understanding the differences of integrins as FMDV receptors among dromedary camel and other species.
authors.affiliation: Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangpu, Chengguan District, Lanzhou 730046, China.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Du
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Larska
authors.affiliation: Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangpu, Chengguan District, Lanzhou 730046, China. Electronic address: changhuiyun@126.com.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Chang
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. Electronic address: Soren.alexandersen@inspection.gc.ca.
authors.forename: Soren
authors.initials: S
authors.lastname: Alexandersen
authors.affiliation: Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangpu, Chengguan District, Lanzhou 730046, China. Electronic address: caixp@public.lz.gs.cn.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D002162:Camelus / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D016023:Integrins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D019030:Receptors, Vitronectin / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D000951:Antigens, Neoplasm; D016023:Integrins; D019030:Receptors, Vitronectin; C094340:integrin alphavbeta1; C073878:integrin alphavbeta6