Enzymatic degradation products from a marine polysaccharide YCP with different immunological activity and binding affinity to macrophages, hydrolyzed by alpha-amylases from different origins.
YCP is a marine polysaccharide with anti-tumor and immune-modulating effects. This study evaluated the effect of enzymatic degradation of YCP by alpha-amylases from different origins on its immunological activity and binding ability to the macrophages. YCP was hydrolyzed by alpha-amylases isolated from Aspergillus oryzae, Bacillus licheniformis, Barley malt, and Porcine pancreas respectively, then four fragments with unique molecular weight (termed: YCP-Ao, YCP-Bl, YCP-Bm, and YCP-Pp, respectively) were obtained. The four fragments showed different immunological activity and the ability to bind to macrophages. Among them, YCP-Ao possessed almost equivalent immunological activity compared to the original YCP, while such properties were not retained in YCP-Bl. Our further study showed that YCP-Ao prevented YCP from binding to macrophages. In conclusion, YCP-Ao and YCP might have similar active regions.
Ren
Min
M
School of Life Science and Technology, China Pharmaceutical University, Tongjia Lane, Zhongyang Road, Nanjing, Jiangsu, PR China.
Yan
Wei
W
Yao
Wenbing
W
Jin
Lei
L
Gao
Xiangdong
X
D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001467:Hordeum / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D011134:Polysaccharides / Q000276:immunology* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D000516:alpha-Amylases / Q000378:metabolism*
D011134:Polysaccharides; D000516:alpha-Amylases