X-ray photoelectron spectroscopy analysis of gold surfaces after removal of thiolated DNA oligomers by ultraviolet/ozone treatment.
Well-ordered films of molecular DNA can be formed by the attachment of thiolated DNA oligonucleotides to a supporting gold substrate. The gold substrate represents a significant fraction of the total cost of preparing such films, and it is thus important to determine whether such substrates can be reused. Here, we investigate with X-ray photoelectron spectroscopy the suitability of UV/ozonolysis previously employed to remove alkanethiols from gold, for removing 40-mer, single- and double-stranded synthetic DNA. We find that while UV/O(3) can indeed remove thiolated DNA from gold slides, the treatment times required permit the implantation of additional organic contaminants.
Mirsaleh-Kohan
Nasrin
N
Département de médecine nucléaire et radiobiologie, Faculté de médecine et les sciences de santé, Université de Sherbrooke, Sherbrooke, QC Canada J1H 5N4. nasrin.mirsaleh@usherbrooke.ca
Bass
Andrew D
AD
Sanche
Léon
L
D000327:Adsorption; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D006046:Gold / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D010126:Ozone / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry* / Q000302:isolation & purification*; D013499:Surface Properties; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D006878:Hydroxides; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D013438:Sulfhydryl Compounds; D010126:Ozone; D013455:Sulfur; D006046:Gold; C031356:hydroxide ion