title: Comparative Measurement of In Vitro T-2 Toxin Cytotoxicity using Three Different Cytotoxicity Assays.
abstract: T-2 toxin is a secondary fungal metabolite produced by various species of fusarium. In this study, the in vitro cytotoxicity of T-2 toxin in the human hepatoma cell line (HepG2) was determined using three methods, including neutral red dye adsorption, trypan blue dye exclusion, and uptake of radiolabeled 3 H-thymidine. The 50% lethal concentration of T-2 toxin was found to be similar for all three, accounting for 2.39, 2.96, and 3.44 ng/mL, respectively, as determined by these assays. Significant correlations were also observed among the three methods (p <. 05 through p <. 005). These results suggest that all three methods are reliable and are comparable for determining the 50% lethal concentration of T-2 toxin. It is concluded that the neutral red cytotoxicity assay may be preferable to other methods because of its simplicity, its use of nonradioactive materials, and its objectivity.
authors.affiliation: Department of Immunology, School of Public Health, and Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, and Avesina Research Center, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Heidari
authors.forename: Mahmmoud
authors.initials: M
authors.lastname: Ghazi-Khansari
authors.forename: Jalal
authors.initials: J
authors.lastname: Khoshnoodi
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri