A one-step method to fabricate PLLA scaffolds with deposition of bioactive hydroxyapatite and collagen using ice-based microporogens.
Porous poly(l-lactic acid) (PLLA) scaffolds with bioactive coatings were prepared by a novel one-step method. In this process, ice-based microporogens containing bioactive molecules, such as hydroxyapatite (HA) and collagen, served as both porogens to form the porous structure and vehicles to transfer the bioactive molecules to the inside of PLLA scaffolds in a single step. Based on scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy analysis, the bioactive components were found to be transferred successfully from the porogens to PLLA scaffolds evenly. Osteoblast cells were used to evaluate the cellular behaviors of the composite scaffolds. After culturing for 8days, MTT assay and alkaline phosphatase activity results suggested that HA/collagen could improve the interactions between osteoblast cells and the polymeric scaffold.
Li
Jiashen
J
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.
Chen
Yun
Y
Mak
Arthur F T
AF
Tuan
Rocky S
RS
Li
Lin
L
Li
Yi
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000737:chemistry*; D007053:Ice; D019344:Lactic Acid; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010316:Particle Size; D011091:Polyesters; D011108:Polymers; D016062:Porosity; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D007053:Ice; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite