Direct-imaging-based quantification of Bacillus cereus ATCC 14579 population heterogeneity at a low incubation temperature.
Bacillus cereus ATCC 14579 was cultured in microcolonies on Anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. Eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells.
den Besten
Heidy M W
HM
Wageningen University and Research Centre, Laboratory of Food Microbiology, Wageningen, The Netherlands. heidy.denbesten@wur.nl
Garcia
Diego
D
Moezelaar
Roy
R
Zwietering
Marcel H
MH
Abee
Tjakko
T
D000222:Adaptation, Physiological / Q000235:genetics; D001409:Bacillus cereus / Q000235:genetics / Q000254:growth & development*; D003080:Cold Temperature; D015169:Colony Count, Microbial / Q000379:methods; D003470:Culture Media; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008856:Microscopy, Fluorescence; D012034:Refrigeration / Q000379:methods
D003470:Culture Media