Profiling of drug action using reporter mice and molecular imaging.
Reporter mice associated to molecular imaging represent a major asset for the study of the spatio-temporal effects of drugs in living animals. The field is still relatively young and so far the number of animals genetically modified to express a given reporter gene ubiquitously and under the control of specific drugs is still limited. For a reporter animal the indispensable elements for the application to drug research and development are (i) the short life of the reporter enabling to have a clear view of the onset as well as the termination of drug effects, (ii) the generalized, drug-dependent activation of the reporter, and (iii) imaging modality suitable for high-throughput analysis. Because of its relative cheapness and ease to perform, in addition to all the above considerations, bioluminescence-based imaging is now regarded as the best imaging technology to be applied to the field of drug research. We show here the application of reporter mouse systems for drug screening in living animals in order to compare drug potency on target and specificity of action.
Rando
Gianpaolo
G
Center of Excellence on Neurodegenerative Diseases and Department of Pharmacological Sciences, University of Milan, Milan, Italy.
Biserni
Andrea
A
Ciana
Paolo
P
Maggi
Adriana
A
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D020847:Estrogen Receptor Modulators / Q000378:metabolism; D005260:Female; D049454:Firefly Luciferin / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000378:metabolism; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D020847:Estrogen Receptor Modulators; D049408:Luminescent Agents; D004364:Pharmaceutical Preparations; D049454:Firefly Luciferin; D008156:Luciferases