Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter -597/-572/-174Haplotypes.
BACKGROUND
Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G-->A), -572(G-->C) and -174(G-->C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 -597/-572/-174haplotyping are elaborate and require large amounts of genomic DNA.


RESULTS
We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions.


CONCLUSIONS
This enables a fast and efficient determination of IL-6 -597/-572/-174haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts.
Müller-Steinhardt
Michael
M
Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Friedrich-Ebert-Strasse 107, 68167 Mannheim, Germany, DRK-Blutspendedienst Baden-Württemberg - Hessen, Germany. m.mueller-steinhardt@blutspende.de.
Schulte
Friederike
F
Klüter
Harald
H
Bugert
Peter
P