An expanded genetic linkage map of an intervarietal Agaricus bisporus var. bisporusxA. bisporus var. burnettii hybrid based on AFLP, SSR and CAPS markers sheds light on the recombination behaviour of the species.
A genetic linkage map for the edible basidiomycete Agaricus bisporus was constructed from 118 haploid homokaryons derived from an intervarietal A. bisporus var. bisporus x A. bisporus var. burnettii hybrid. Two hundred and thirty-one AFLP, 21 SSR, 68 CAPS markers together with the MAT, BSN, PPC1 loci and one allozyme locus (ADH) were evenly spread over 13 linkage groups corresponding to the chromosomes of A. bisporus. The map covers 1156cM, with an average marker spacing of 3.9cM and encompasses nearly the whole genome. The average number of crossovers per chromosome per individual is 0.86. Normal recombination over the entire genome occurs in the heterothallic variety, burnettii, contrary to the homothallic variety, bisporus, which showed adaptive genome-wide suppressed recombination. This first comprehensive genetic linkage map for A. bisporus provides foundations for quantitative trait analyses and breeding programme monitoring, as well as genome organisation studies.
Foulongne-Oriol
Marie
M
Mycologie et Sécurité des Aliments, INRA, Centre de Recherche Bordeaux-Aquitaine, Villenave d'Ornon Cedex, France. mfoulong@bordeaux.inra.fr
Spataro
Cathy
C
Cathalot
Vincent
V
Monllor
Sarah
S
Savoie
Jean-Michel
JM
D000364:Agaricus / Q000235:genetics*; D054458:Amplified Fragment Length Polymorphism Analysis; D002678:Chimera / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics; D019143:Evolution, Molecular; D049770:Genes, Mating Type, Fungal; D008040:Genetic Linkage; D005819:Genetic Markers; D016681:Genome, Fungal; D023281:Genomics; D005838:Genotype; D007621:Karyotyping; D018895:Microsatellite Repeats; D008957:Models, Genetic; D010641:Phenotype; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D005819:Genetic Markers