title: The covalent immobilization of heparin to pulsed-plasma polymeric allylamine films on 316L stainless steel and the resulting effects on hemocompatibility.
abstract: For an improved hemocompatibility of 316L stainless steel (SS), we develop a facile and effective approach to fabricating a pulsed-plasma polymeric allylamine (P-PPAm) film that possesses a high cross-linking degree and a high density of amine groups, which is used for subsequent bonding of heparin. The P-PPAm film as a stent coating shows good resistance to the deformation behavior of compression and expansion of a stent. Using deionized water as an aging medium, it is demonstrated that the heparin-immobilized P-PPAm (Hep-P-PPAm) surface has a good retention of heparin. The systematic in vitro hemocompatibility evaluation reveals lower platelet adhesion, platelet activation and fibrinogen activation on the Hep-P-PPAm surface, and the activated partial thromboplastin time prolongs for about 15 s compared with 316L SS. The P-PPAm surface significantly promotes adhesion and proliferation of endothelial cells (ECs). For the Hep-P-PPAm, although EC adhesion and proliferation is slightly suppressed initially, after cultivation for 3 days, the growth behavior of ECs is remarkably improved over 316L SS. In vivo results indicate that the Hep-P-PPAm surface successfully restrain thrombus formation by growing a homogeneous and intact shuttle-like endothelium on its surface. The Hep-P-PPAm modified 316L SS shows a promising application for vascular devices.
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Maitz
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Jing
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000499:Allylamine / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D006461:Hemolysis; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D015539:Platelet Activation / Q000502:physiology; D010973:Platelet Adhesiveness / Q000502:physiology; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013193:Stainless Steel / Q000737:chemistry*; D015607:Stents; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D013193:Stainless Steel; D000499:Allylamine; D005340:Fibrinogen; D006493:Heparin