Monodispersed submicron porous silica particles functionalized with CD derivatives for chiral CEC.
Rapid and efficient enantioseparation of halogen aryl alcohols and beta-blockers propranolol and pindolol in packed bed CEC (p-CEC) using as-prepared submicron porous silica chiral stationary phases (CSPs) has been achieved. Monodispersed 0.66 and 0.81 microm chiral submicron porous silica spheres were prepared using tetramethoxysilane and hexadecyltrimethylammonium bromide, followed by a hydrothermal treatment method with ammonia-ethanol to expand the pore of silica spheres without changing their spherical morphology. A proper specific surface of ca. 230 m(2)/g and pore sizes average of 6-8 nm were obtained by this method. The submicron porous silica spheres were modified with mono-6-phenylcarbamoylated beta-CD via thiol-en radical addition. They were packed into 9 cm 50 microm id capillary columns with photopolymerized monolithic frits. These submicron CSPs showed greater column efficiency (about 476 000 plates/m for 4-iodophenyl-1-ethanol) and higher resolution than the corresponding 3 microm CSP.
Li
Lai-Sheng
LS
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
Wang
Yong
Y
Young
David James
DJ
Ng
Siu-Choon
SC
Tan
Timothy Thatt Yang
TT
D000327:Adsorption; D000438:Alcohols / Q000737:chemistry; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006219:Halogens / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009584:Nitrogen / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000438:Alcohols; D006219:Halogens; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; D009584:Nitrogen