title: Assessment of the reproducibility of random hexapeptide peptide library-based protein normalization.
abstract: The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.
authors.affiliation: Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg MB, Canada.
authors.forename: Ravi C
authors.initials: RC
authors.lastname: Dwivedi
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Krokhin
authors.forename: John P
authors.initials: JP
authors.lastname: Cortens
authors.forename: John A
authors.initials: JA
authors.lastname: Wilkins
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009842:Oligopeptides / Q000737:chemistry*; D019151:Peptide Library; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D011506:Proteins; D020543:Proteome