title: FCS-based sensing for the detection of ochratoxin and neomycin in food.
abstract: In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.
authors.affiliation: Laboratory for Molecular Sensing, IBP-CNR, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Varriale
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Luisa
authors.initials: L
authors.lastname: Iozzino
authors.forename: Lorella
authors.initials: L
authors.lastname: Severino
authors.forename: Aniello
authors.initials: A
authors.lastname: Anastasio
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Cortesi
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D005455:Fluorescent Antibody Technique; D005506:Food Contamination / Q000032:analysis*; D009355:Neomycin / Q000032:analysis* / Q000276:immunology; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000906:Antibodies; D009793:Ochratoxins; C025589:ochratoxin A; D009355:Neomycin