Immune response and expression analysis of cathepsin K in goldfish during Aeromonas hydrophila infection.
The innate immunity and expression profiles of cathepsins D were determined in the goldfish (Carassius auratus) tissues after challenge with a fish pathogen Aeromonas hydrophila. The innate immunity of reactive oxygen species (ROS) and reactive nitrogen species (RNS) were determined by peripheral blood leucocytes. Blood and tissue samples of the muscle, gills, liver, kidney, heart, spleen, and intestine were sampled at 1, 3, 6 and 12 h post-infection for cathepsin D expression by semi-quantitative RT-PCR. The ROS and RNS production did not significantly increase at 1 h post-challenged goldfish. However, the ROS and RNS production was significantly increased after 3 h post-challenged fish compared to the control. The cathepsin D expression was found very low in muscle and kidney of the control fish, other tissues was not found the expression. A similar pattern was found in goldfish at 1 h post-challenge with A. hydrophila. However, at 3 h post-challenge goldfish, the cathepsin D expression was high only in the heart. At 6 h post-challenge goldfish, the cathepsin D expression was seen high all the tissues, except in the spleen. However, the expression was decreased at 12 h post-infection samples. This result was suggested that the goldfish infected with A. hydrophila decreased the innate immunity level in peripheral blood and expressed the cathepsin D in tissues.
Harikrishnan
Ramasamy
R
Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea. rhari123@yahoo.com
Kim
Man-Chul
MC
Kim
Ju-Sang
JS
Han
Young-Jae
YJ
Jang
Ik-Soo
IS
Balasundaram
Chellam
C
Heo
Moon-Soo
MS
D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D056657:Cathepsin K / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000401:mortality; D020869:Gene Expression Profiling / Q000662:veterinary; D006054:Goldfish / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D007113:Immunity, Innate; D017382:Reactive Oxygen Species / Q000276:immunology
D017382:Reactive Oxygen Species; D056657:Cathepsin K