Retinal phototoxicity in a novel murine model of intraocular lens implantation.
OBJECTIVE
To establish a novel murine intraocular lens (IOL) implantation model to study the protective effects of colored-IOLs against retinal phototoxicity.


METHODS
Two-millimeter diameter IOL buttons were created from IOLs for clinical use. Extra-capsular crystalline lens extraction and IOL implantation were performed in BALB/c mice using a technique similar to human cataract surgery. For light exposure experiments, mice were exposed to 5,000 LUX of white light for 24 h on the day after surgery. To investigate the protective effects of yellow IOL against light exposure, ERG measurements were conducted in vivo, followed by TdT-mediated dUTP Nick-End Labeling (TUNEL) and outer nuclear layer (ONL) thickness measurement of retinal tissue in yellow or clear IOL-implanted mice and control mice without surgery.


RESULTS
IOLs were successfully implanted in all animals, and IOL buttons without haptics were well stabilized in the capsular bag. Murine eyes developed posterior capsule opacification (PCO) after IOL implantation by postoperative day 5 at the latest. In contrast to the clear IOL-implanted animals stimulated by light exposure, the yellow IOL-implanted animals had significantly reduced numbers of TUNEL-positive cells and retained thickness of the ONL. The ERG showed that yellow IOL implantation prevents a decrease of amplitude in both the a-wave and b-wave compared with clear IOL implantation.


CONCLUSIONS
We established a new animal model of IOL implantation and demonstrated the protective effects of colored-IOL against retinal phototoxicity after cataract surgery.
Kurihara
Toshihide
T
Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan.
Omoto
Masahiro
M
Noda
Kousuke
K
Ebinuma
Mari
M
Kubota
Shunsuke
S
Koizumi
Haruna
H
Yoshida
Satoru
S
Ozawa
Yoko
Y
Shimmura
Shigeto
S
Ishida
Susumu
S
Tsubota
Kazuo
K
D000818:Animals; D002386:Cataract / Q000150:complications / Q000473:pathology; D004195:Disease Models, Animal; D006801:Humans; D007910:Lenses, Intraocular; D008027:Light / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019919:Prosthesis Implantation; D012160:Retina / Q000473:pathology* / Q000528:radiation effects* / Q000601:surgery; D012162:Retinal Degeneration / Q000150:complications / Q000473:pathology