title: Simocyclinone D8 turns on against Gram-negative bacteria in a clinical setting.
abstract: Simocyclinone D8 (SD8) is known to affect Gram-positive bacteria only. By testing SD8 against several clinical isolates, we showed that SD8 resulted very active against Gram-negative bacteria from clinical specimens, while it was shown inactive against laboratory strains. The activity against the former was in part due to enhanced drug entry. In addition, SD8 appears to share chromosome- and plasmid-mediated resistance mechanisms with fluoroquinolones.
authors.affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, via Gabelli 63, 35121 Padova, Italy. sara.richter@unipd.it
authors.forename: Sara N
authors.initials: SN
authors.lastname: Richter
authors.forename: Ilaria
authors.initials: I
authors.lastname: Frasson
authors.forename: Manlio
authors.initials: M
authors.lastname: Palumbo
authors.forename: Claudia
authors.initials: C
authors.lastname: Sissi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D048168:Escherichia coli K12 / Q000187:drug effects / Q000254:growth & development; D024841:Fluoroquinolones / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D003374:Coumarins; D024841:Fluoroquinolones; D006027:Glycosides; C416814:simocyclinone D8