Toxicity of purified terephthalic acid manufacturing wastewater on reproductive system of male mice (Mus musculus).
Reproductive toxicity of purified terephthalic acid (PTA) manufacturing wastewater on the male mice (Mus musculus) was investigated after 35-day intragastric perfusion treatment with the wastewater. Fluorescein diacetate and propidium iodide staining, and flow cytometry were used to assess the toxicity of PTA wastewater on spermatogenic cells. PTA wastewater induced significant variations in the relative percentages of immature haploid, diploid, tetraploid and S-phase spermatogonia. Percentage of viable spermatogenic cells was reduced from 93.1+/-2.3 in control group to 90.4+/-1.9 in the wastewater-treated group. Testicular histopathology revealed expansion of interstitial space and reduction in the number and size of Leydig cells induced by the wastewater, which was further certified by the decrease (10.6%) in relative testes weight and the increase (101.3%) in sperm shape abnormality in the wastewater-treated group. In this study, PTA wastewater was found to have reproductive toxicity on male mice, and public health problems may potentially arise from the discharge of the wastewater into the environment.
Zhang
Xu-Xiang
XX
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Institute of Environmental Health & Ecological Security, Nanjing University, Nanjing, Jiangsu 210093, China. zhangxx@nju.edu.cn
Sun
Shi-Lei
SL
Zhang
Yan
Y
Wu
Bing
B
Zhang
Zong-Yao
ZY
Liu
Bo
B
Yang
Liu-Yan
LY
Cheng
Shu-Pei
SP
D000818:Animals; D016166:Free Radical Scavengers; D005837:Genitalia, Male / Q000187:drug effects; D007220:Industrial Waste / Q000009:adverse effects*; D007985:Leydig Cells / Q000473:pathology; D008297:Male; D008420:Manufactured Materials; D051379:Mice; D010795:Phthalic Acids; D013093:Spermatogonia / Q000187:drug effects*; D013737:Testis / Q000473:pathology
D016166:Free Radical Scavengers; D007220:Industrial Waste; D010795:Phthalic Acids; C011363:terephthalic acid