title: Metabolic turnover analysis by a combination of in vivo 13C-labelling from 13CO2 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C3 photosynthetic pathway in Nicotiana tabacum leaves.
abstract: Understanding of the control of metabolic pathways in plants requires direct measurement of the metabolic turnover rate. Sugar phosphate metabolism, including the Calvin cycle, is the primary pathway in C(3) photosynthesis, the dynamic status of which has not been assessed quantitatively in the leaves of higher plants. Since the flux of photosynthetic carbon metabolism is affected by the CO(2) fixation rate in leaves, a novel in vivo (13)C-labelling system was developed with (13)CO(2) for the kinetic determination of metabolic turnover that was the time-course of the (13)C-labelling ratio in each metabolite. The system is equipped with a gas-exchange chamber that enables real-time monitoring of the CO(2) fixation rate and a freeze-clamp that excises a labelled leaf concurrently with quenching the metabolic reactions by liquid nitrogen within the photosynthesis chamber. Kinetic measurements were performed by detecting mass isotopomer abundance with capillary electrophoresis-tandem mass spectrometry. The multiple reaction monitoring method was optimized for the determination of each compound for sensitive detection because the amount of some sugar phosphates in plant cells is extremely small. Our analytical system enabled the in vivo turnover of sugar phosphates to be monitored in fresh tobacco (Nicotiana tabacum) leaves, which revealed that the turnover rate of glucose-1-phosphate (G1P) was significantly lower than that of other sugar phosphates, including glucose-6-phosphate (G6P). The pool size of G1P is 12 times lower than that of G6P. These results indicate that the conversion of G6P to G1P is one of the rate-limiting steps in the sugar phosphate pathway.
authors.affiliation: Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizugawa-shi, Kyoto 619-0292 Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Miyazawa
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Chikahiro
authors.initials: C
authors.lastname: Miyake
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D019298:Glucose-6-Phosphate / Q000737:chemistry / Q000378:metabolism; D005958:Glucosephosphates / Q000737:chemistry / Q000378:metabolism; D007553:Isotope Labeling; D007700:Kinetics; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D005958:Glucosephosphates; D002245:Carbon Dioxide; D019298:Glucose-6-Phosphate; C031590:glucose-1-phosphate