Studies on bovine cervical mucin. I. The amino acid composition and N-terminal amino acid of bovine oestrus cervical mucin.
Bovine oestrus cervical mucin, isolated by gel filtration on Sepharose 6B, was found to be homogeneous (i) in electrophoresis on cellulose acetate at pH 8.6 after release of sialic acid and in acrylamide-agarose gel at pH 8.7 in the presence of sodium dodecyl sulphate, where not a single compact zone was found after staining for proteins, (ii) by analytical ultracentrifugation and equilibrium centrifugation in a CsCl density gradient; as well as (iii) by immunoelectrophoresis, which revealed two kinds of antibodies against mucin, reacting, respectively, with the primary structure and with determinants depending on the integrity of the glycoprotein structure. Alanine was identified as the N-terminal amino acid of the peptide core, which appeared to consist of a repetition of a 32-35 residue sequence, viz. (Thr)8, (Glu, Pro, Ala, Val)3, (Asp, Leu)2 and (Arg, Cys, Ileu)1. No integral numbers could be assigned to (Ser)3.4, (Gly)2.3 and (Phe)0.6 which were also present.
Bhushana Rao
K S
KS
Department of Experimental Medicine and Physiology of Human Reproduction Research Unit, University of Louvain, Avenue Chapelle-aux-Champs 4, B-1200, Belgium.
Van Roost
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E
Masson
P L
PL
Heremans
J F
JF
Andre
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F
D000178:Acrylamides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002584:Cervix Uteri / Q000378:metabolism*; D002586:Cesium / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004971:Estrus / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D009077:Mucins / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012685:Sepharose / Q000737:chemistry; D014461:Ultracentrifugation / Q000379:methods
D000178:Acrylamides; D000596:Amino Acids; D002712:Chlorides; D003432:Cross-Linking Reagents; D009077:Mucins; D002586:Cesium; D012685:Sepharose; D010447:Peptide Hydrolases; C028019:cesium chloride