Evaluation of genotoxicity of Yanhuanglian dehydrocavidine (YHL-DC) in vitro and in vivo.
It is reported that dehydrocavidine (DC), the main component of a traditional Chinese medicine, Yanhuanglian (YHL), can protect hepatic tissue against HBV and HAV impairment. As part of a safety evaluation on YHL-DC for use in the treatment of HBV, the present study evaluated the potential genotoxicity of YHL-DC by using the standard battery of tests (i.e., bacterial reverse mutation, chromosomal aberrations, and mouse micronucleus assays) recommended by the State Food and Drug Administration of China. The results showed that YHL-DC was not genotoxic under the conditions of the reverse mutation, chromosomal aberrations, and mouse micronucleus assay conditions. The anticipated clinical dose should be smaller than the doses used in the genotoxicity assays. With confirmation from further toxicity studies, YHL-DC would hopefully prove to be a useful anti-HBV agent.
Jiangbo
Zhu
Z
Department of Toxicology, Second Military Medical University, Shanghai, China.
Xuying
Wan
W
Yuping
Zhu
Z
Xili
Ma
M
Tianbao
Zhang
Z
D000818:Animals; D001600:Berberine Alkaloids / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D010936:Plant Extracts; D014481:United States
D001600:Berberine Alkaloids; D009153:Mutagens; D010936:Plant Extracts; C011188:cavidine