Metabolic intervention of aflatoxin B1 toxicity by curcumin.
BACKGROUND
Curcumin, bioactive principle of turmeric (Curcuma longa Linn) is an important constituent of Indian traditional medicine. Turmeric has been known to possess several therapeutic properties.


OBJECTIVE
The modulatory effect of dietary curcumin (0.05%, w/w) on drug metabolizing and general marker enzymes of liver and formation of AFB(1)-adducts (DNA and protein) due to dietary AFB(1) exposure for a period of 6 weeks in a rodent model, have been evaluated.


METHODS
Drug metabolizing enzymes CYP1A1, GSHT, UGT1A and general marker enzymes (LDH, ALT, AST, ALP and gamma-GT) of liver were estimated by standardized methods. Aflatoxin adducts (DNA and protein) were quantitated by indirect competitive ELISA.


RESULTS
Dietary curcumin enhanced GSHT (p<0.001) and UGT1A1 (p<0.05) activity and significantly reduced the activity of CYP1A1 (p<0.001), in rats exposed to aflatoxin B(1). Supplementation of curcumin in the diet normalized the altered activities of LDH and ALT. At molecular level, curcumin significantly reduced AFB(1)-N(7)-guanine adduct (p<0.001) excretion in the urine, DNA adduct (p<0.05) in the liver and albumin adduct (p<0.001) in the serum.


CONCLUSIONS
The experimental results substantiates that curcumin intervention ameliorates the AFB(1) induced toxicity.
Nayak
Sujatha
S
Department of Biochemistry, University College of Science, Osmania University, Hyderabad, AP, India.
Sashidhar
R B
RB
D016604:Aflatoxin B1 / Q000493:pharmacokinetics / Q000633:toxicity*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D018736:DNA Adducts / Q000378:metabolism; D019587:Dietary Supplements; D004798:Enzymes / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D008658:Inactivation, Metabolic; D008099:Liver / Q000201:enzymology*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats
D000418:Albumins; D000975:Antioxidants; D018736:DNA Adducts; D004798:Enzymes; D010936:Plant Extracts; D006147:Guanine; C012596:turmeric extract; D016604:Aflatoxin B1; D003474:Curcumin