Pretreatment of poultry litter improves Bacillus thuringiensis-based biopesticides production.
Pretreated poultry litter was used in batch cultures for the production of Bacillus thuringiensis (Bt)-based biopesticide of lepidoptera- and diptera-specific Cry1 and Cry2, diptera-specific Cry4Ba and Cry11Aa and coleoptera-specific Cry3Aa toxins by Bt subsp. kurstaki 81, subsp. israelensis HD500 and subsp. tenebrionis 3203, respectively. Bt kurstaki 81 showed improved growth and produced more toxin in this medium as compared to other subspecies. Base and acid hydrolysis were tested as the methods of substrate pretreatment. The use of poultry litter pretreated with 2N HCl yielded 94% more bioinsecticidal protein than 2N NaOH-pretreated poultry litter when Bt kurstaki 81 was cultured. With appropriate pretreatment, poultry litter demonstrated potential as a valuable raw material for a low-cost complex medium to produce Bt-based biopesticides.
Ozcan
Orhan
O
Middle East Technical University, Department of Biological Sciences, 06531 Ankara, Turkey.
Icgen
Bulent
B
Ozcengiz
Gulay
G
D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002244:Carbon / Q000032:analysis; D004731:Endotoxins / Q000096:biosynthesis*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D009584:Nitrogen / Q000032:analysis; D010572:Pest Control, Biological / Q000379:methods*; D011200:Poultry; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D014866:Waste Products / Q000032:analysis*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D014866:Waste Products; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D002244:Carbon; D009584:Nitrogen