title: Single-gene transgenic mouse strains for reprogramming adult somatic cells.
abstract: We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: Bryce W
authors.initials: BW
authors.lastname: Carey
authors.forename: Styliani
authors.initials: S
authors.lastname: Markoulaki
authors.forename: Caroline
authors.initials: C
authors.lastname: Beard
authors.forename: Jacob
authors.initials: J
authors.lastname: Hanna
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D000818:Animals; D054337:Cell Dedifferentiation / Q000187:drug effects; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects / Q000235:genetics*; D004318:Doxycycline / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016678:Genome; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016254:Mutagenesis, Insertional; D009928:Organ Specificity; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics*
chemical_list: C045073:Cre recombinase; D019426:Integrases; D004318:Doxycycline