title: A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report.
abstract: OBJECTIVE
  To determine whether CX(3)CR1(+) bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation.
  
  
  RESULTS
  CX(3)CR1(+) and CX(3)CR1(-) cells were isolated from marrow of CX(3)CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX(3)CR1(+) bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC.
  
  
  CONCLUSIONS
  CX(3)CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.
authors.affiliation: Centre for Research in Vascular Biology, BioSciences Institute, University College Cork, College Road, Cork City, Ireland.
authors.forename: Pat
authors.initials: P
authors.lastname: Metharom
authors.forename: Arun H S
authors.initials: AH
authors.lastname: Kumar
authors.forename: Sharon
authors.initials: S
authors.lastname: Weiss
authors.forename: Noel M
authors.initials: NM
authors.lastname: Caplice
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D020013:Calcium Signaling; D002454:Cell Differentiation; D002478:Cells, Cultured; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009000:Monocytes / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D019707:Receptors, Chemokine