title: Influence of IL-1 gene polymorphism on the periodontal microbiota of HIV-infected Brazilian individuals.
abstract: This study investigated the association of IL-1A (+4845) and IL-1B (+3954) gene polymorphism with the subgingival microbiota and periodontal status of HIV-infected Brazilian individuals on highly active antiretroviral therapy (HAART). One hundred and five subjects were included in the study, distributed into 2 HIV groups [29 chronic periodontitis (CP+) and 30 periodontally healthy (H+)]; and 2 non-HIV groups (29 CP- and 17 H- patients). IL-1A and B were genotyped by PCR and restriction enzyme digestion. Thirty-three bacterial species were detected by checkerboard. Overall, we observed a prevalence of the allele 2 in the IL1-A and IL-1B polymorphism at 30.5% and 25.7%, respectively. Only 11.4% of all patients were composite genotype-positive, and 75% of those were HIV-infected. No significant associations between polymorphism of the IL-1 gene and periodontitis or HIV infection were observed. Likewise, no significant differences in the frequency and counts of any bacterial species were found between individuals with and without allele 2 (IL-1A or IL-1B). The data indicated that the IL-1 gene polymorphism is neither associated with periodontal destruction nor with high levels of subgingival species, including putative periodontal pathogens in HIV Brazilian individuals on HAART.
authors.affiliation: Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil.
authors.forename: Lucio de Souza
authors.initials: LS
authors.lastname: Gonçalves
authors.forename: Sônia Maria Soares
authors.initials: SM
authors.lastname: Ferreira
authors.forename: Celso Oliveira
authors.initials: CO
authors.lastname: Souza
authors.forename: Ana Paula Vieira
authors.initials: AP
authors.lastname: Colombo
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D001419:Bacteria / Q000145:classification; D001938:Brazil; D055113:Chronic Periodontitis / Q000382:microbiology*; D004812:Epidemiologic Methods; D005838:Genotype; D005881:Gingiva / Q000382:microbiology*; D015658:HIV Infections / Q000188:drug therapy*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D007375:Interleukin-1; D053582:Interleukin-1alpha; D053583:Interleukin-1beta