Lineage-specific effects of Notch/Numb signaling in post-embryonic development of the Drosophila brain.
Numb can antagonize Notch signaling to diversify the fates of sister cells. We report here that paired sister cells acquire different fates in all three Drosophila neuronal lineages that make diverse types of antennal lobe projection neurons (PNs). Only one in each pair of postmitotic neurons survives into the adult stage in both anterodorsal (ad) and ventral (v) PN lineages. Notably, Notch signaling specifies the PN fate in the vPN lineage but promotes programmed cell death in the missing siblings in the adPN lineage. In addition, Notch/Numb-mediated binary sibling fates underlie the production of PNs and local interneurons from common precursors in the lAL lineage. Furthermore, Numb is needed in the lateral but not adPN or vPN lineages to prevent the appearance of ectopic neuroblasts and to ensure proper self-renewal of neural progenitors. These lineage-specific outputs of Notch/Numb signaling show that a universal mechanism of binary fate decision can be utilized to govern diverse neural sibling differentiations.
Lin
Suewei
S
Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Lai
Sen-Lin
SL
Yu
Huang-Hsiang
HH
Chihara
Takahiro
T
Luo
Liqun
L
Lee
Tzumin
T
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D007150:Immunohistochemistry; D007605:Juvenile Hormones / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D050809:POU Domain Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D051880:Receptors, Notch / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
D029721:Drosophila Proteins; D007605:Juvenile Hormones; C497759:N protein, Drosophila; D009419:Nerve Tissue Proteins; D050809:POU Domain Factors; D051880:Receptors, Notch; C060606:numb protein, Drosophila; C068431:acj6 protein, Drosophila