Bio-resolution of glycidyl (o, m, p)-methylphenyl ethers by Bacillus megaterium.
A newly isolated Bacillus megaterium with epoxide hydrolase activity resolved racemic glycidyl (o, m, p)-methylphenyl ethers to give enantiopure epoxides in 84-99% enantiomeric excess and with 21-73 enantiomeric ratios. The (S)-enantiomer was obtained from rac-glycidyl (o or m)-methylphenyl ether while the (R)-epoxides was obtained from glycidyl p-methylphenyl ether. The observations are explained at the level by enzyme-substrate docking studies.
Zhang
Zhengfang
Z
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014 People's Republic of China.
Sheng
Yanming
Y
Jiang
Keyi
K
Wang
Zhao
Z
Zheng
Yuguo
Y
Zhu
Qing
Q
D000840:Anisoles / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001410:Bacillus megaterium / Q000737:chemistry / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D013237:Stereoisomerism
D000840:Anisoles; D004852:Epoxy Compounds; C020344:glycidyl ethers