title: Phospholipase and proteinase activities of Candida isolates from denture wearers.
abstract: The aim of the present study was to characterise phospholipase and proteinase activities of oral Candida isolates from 100 denture wearers and to study the relationship of these activities with denture stomatitis. Of 100 patients studied, 44 suffered from denture stomatitis. Specimens were collected by swabbing the denture and underlying mucosa. Isolates were previously identified by conventional mycological and genotypic methods. The phospholipase and proteinase activities were evaluated by agar plate methods. A total of 152 isolates were recovered from denture and underlying mucosa, including 101 Candida albicans, 18 Candida tropicalis, 14 Candida glabrata, 11 Candida guilliermondii, four Candida parapsilosis, two Saccharomyces cerevisiae and one isolate each of Candida dubliniensis and Candida krusei. Most C. albicans (97%) showed phospholipase activity; furthermore, the unique C. dubliniensis isolate showed a moderate phospholipase activity. The isolation of C. albicans (chi-square test, P = 0.0016) and phospholipase production by Candida spp. (chi-square test, P = 0.0213) was found to be significantly associated with denture stomatitis. Proteinase production was observed in <30% of isolates, and it was not related to the presence of denture stomatitis (P = 0.7675). Candida albicans isolates may produce both virulence factors, although the proteinase production was only observed in <30% of the isolates. Phospholipase production was exclusive of C. albicans and C. dubliniensis.
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Marcos-Arias
authors.forename: Elena
authors.initials: E
authors.lastname: Eraso
authors.forename: Lucila
authors.initials: L
authors.lastname: Madariaga
authors.forename: Jose Manuel
authors.initials: JM
authors.lastname: Aguirre
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quindós
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification* / Q000378:metabolism; D003778:Dentures; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000382:microbiology*; D009172:Mycology / Q000379:methods; D010447:Peptide Hydrolases / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D013282:Stomatitis, Denture / Q000382:microbiology*
chemical_list: D010740:Phospholipases; D010447:Peptide Hydrolases