13C-NMR detection of STD spectra.
We have investigated the use of (13)C for the detection of saturation transfer difference (STD) NMR spectra. By detecting the STD spectrum in the (13)C channel it is possible to eliminate the residual water signal in the STD-NMR spectrum. We have employed an INEPT transfer in order to shift the magnetization from the proton channel to (13)C. As a sample system to check our method we have used human serum albumin and phenylalanine. We have shown that such a transfer can be accomplished and gives reasonable signal intensities.
Räuber
Christoph
C
Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany.
Berger
Stefan
S
D002021:Buffers; D002247:Carbon Isotopes / Q000032:analysis* / Q000737:chemistry; D017666:Deuterium Oxide / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010649:Phenylalanine / Q000032:analysis / Q000737:chemistry; D017785:Photons; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry
D002021:Buffers; D002247:Carbon Isotopes; D012709:Serum Albumin; D010649:Phenylalanine; D017666:Deuterium Oxide