Fabrication and evaluation of chiral monolithic column modified by beta-cyclodextrin derivatives.
Based on combination of chiral recognition ability of beta-cyclodextrin (beta-CD) derivatives and flexibility of monolithic material, a series of chiral stationary phases (CSPs) were prepared by the immobilization of beta-CD and three of its derivatives to the epoxy-activated poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith under mild condition. Immobilization condition for the connection reaction by different functional groups and bonding ways was studied to obtain good enantiomer selectivity. Prepared CSPs were evaluated by separating racemic mixtures of eight amino acids and two chiral drugs with capillary electrochromatography (CEC).
Li
Yingjie
Y
Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Xuefu Road 74, Harbin 150080, Nangang District, Heilongjiang, PR China.
Song
Chunhui
C
Zhang
Lingyi
L
Zhang
Weibing
W
Fu
Honggang
H
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D002021:Buffers; D053801:Capillary Electrochromatography / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008768:Methylmethacrylates / Q000737:chemistry; D008801:Mexiletine / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000596:Amino Acids; D002021:Buffers; D008768:Methylmethacrylates; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; D008801:Mexiletine; C020347:GMA-EDMA