title: Solubilization and functional reconstitution of human neuropeptide FF2 receptors.
abstract: Neuropeptide FF (NPFF, FLFQPQRFamide) receptors modulate endogenous opioid functions. Here, we report the solubilization of the human NPFF(2) receptor expressed in Chinese hamster ovary (CHO) cells by the zwitterionic detergent Chaps. Chaps solubilization resulted in the abolishment of specific agonist binding activity, which was restored by a polyethylene glycol (PEG) precipitation method. Reincorporation after the precipitation step into liposomes made of endogenous lipids issued from CHO membranes or exogenous lipids significantly enhanced the specific agonist binding activity and G-protein coupling. This method of solubilization and lipid reconstitution could be useful for studies of NPFF receptors.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, CNRS/Université de Toulouse, UMR 5089, 205 route de Narbonne, Toulouse Cedex, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Talmont
authors.forename: Catherine
authors.initials: C
authors.lastname: Mollereau
authors.forename: Isabelle
authors.initials: I
authors.lastname: Muller
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Zajac
mesh_terms: D000818:Animals; D016466:CHO Cells; D002793:Cholic Acids / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003902:Detergents / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018013:Receptors, Neuropeptide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012995:Solubility
chemical_list: D002793:Cholic Acids; D003902:Detergents; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate