Enrichment of pinolenic acid at the sn-2 position of triacylglycerol molecules through lipase-catalyzed reaction.
Reports have shown that Delta-5 polyunsaturated fatty acids (-5 PUFA) are enriched at sn-1,3 positions of triacylglycerols (TAG) in pine (Pinus koraiensis) nut oil (Pn). As a major Delta-5 PUFA, pinolenic acid (Pi) is about 14.2% in the oil, while the percentage of Pi at the sn-1 and/or sn-3 positions in TAG was found more than 20%. In this current study, the enhancement of Pi at the sn-2 position has been achieved by acyl migration during the lipase-catalyzed inter-esterification between Pn and palm stearin (Ps). After reaction, the proportion of Pi increased at sn-2 positional fatty acid even is similar to that in total fatty acid; for example, in the inter-esterified product of 50:50 (Pn:Ps), the same amount of Pi (7.1%) present was detected both at the sn-2 and sn-1,3 positions. However, the reduction of phytosterols and tocopherols are observed in the inter-esterified products.
Zhu
Xue-Mei
XM
Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, South Korea.
Hu
Jiang-Ning
JN
Shin
Jung-Ah
JA
Li
Dan
D
Jin
Juan
J
Adhikari
Prakash
P
Akoh
Casimir C
CC
Lee
Jeung-Hee
JH
Choi
Sang-Won
SW
Lee
Ki-Teak
KT
D004951:Esterification; D008042:Linolenic Acids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry*; D028223:Pinus / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D024505:Tocopherols / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*
D008042:Linolenic Acids; D010840:Phytosterols; D010938:Plant Oils; D014280:Triglycerides; C034302:5,9,12-octadecatrienoic acid; D008049:Lipase; D024505:Tocopherols