title: Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.
abstract: p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 130062, Changchun, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiuping
authors.initials: X
authors.lastname: Wu
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.forename: Junwen
authors.initials: J
authors.lastname: Fan
authors.forename: Fa
authors.initials: F
authors.lastname: Ge
authors.forename: Xuelin
authors.initials: X
authors.lastname: Wang
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000935:Antifungal Agents / Q000633:toxicity*; D001547:Benzaldehydes / Q000633:toxicity*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D053858:Metabolic Networks and Pathways; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D013223:Statistics as Topic / Q000379:methods
chemical_list: D000935:Antifungal Agents; D001547:Benzaldehydes; C024896:4-anisaldehyde