title: Association of the tumor necrosis factor-alpha -308 G/A polymorphism with nasal polyposis.
abstract: Nasal polyposis (NP) is a chronic inflammatory disease in which several molecular and cellular interactions play important roles. Tumor necrosis factor-alpha (TNF-alpha) is a major pro-inflammatory cytokine with a key role in immune inflammatory responses in NP. Altered levels of TNF-alpha, which may occur due to polymorphisms in the TNF-alpha promoter region, may also be associated with NP susceptibility. Given these facts, we investigated the possible association of the TNF-alpha -308 G/A single nucleotide polymorphism (SNP) with NP. In this study, 97 consecutive adult patients with NP and 95 age- and gender-matched controls were recruited. For identification of SNP, restriction fragment length polymorphism analysis after polymerase chain reaction was carried out. The NP group had a significantly higher rate of polymorphism compared to controls (p = 0.015). Logistic regression analysis revealed that the presence of the TNF-alpha -308 G/A SNP is an independent risk factor for NP development (OR, 3.68; CI, 1.27-10.7; p = 0.016). The presence of a mutation failed to influence disease severity on the basis of resistance to medical and/or surgical treatment. This study suggests a possible linkage of a SNP in the TNF-alpha promoter with NP. These results need to be confirmed with multicentre studies for more precise interpretation and corroborative studies for investigating the influence of polymorphism on transcriptional activity.
authors.affiliation: Otolaryngology Department, Elazig State Hospital, Elazig, Turkey.
authors.forename: Hunkar
authors.initials: H
authors.lastname: Batikhan
authors.forename: Mustafa Kursat
authors.initials: MK
authors.lastname: Gokcan
authors.forename: Esen
authors.initials: E
authors.lastname: Beder
authors.forename: Nejat
authors.initials: N
authors.lastname: Akar
authors.forename: Aysenur
authors.initials: A
authors.lastname: Ozturk
authors.forename: Muharrem
authors.initials: M
authors.lastname: Gerceker
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D001249:Asthma / Q000175:diagnosis / Q000235:genetics; D055963:Asthma, Aspirin-Induced / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000175:diagnosis / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha