aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon.
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
Bell
Jan M
JM
SA Pathology, Women's and Children's Hospital, 72 King William Rd., North Adelaide, SA 5006, Australia.
Turnidge
John D
JD
Andersson
Patiyan
P
D000123:Acetyltransferases / Q000235:genetics*; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D044366:Transition Temperature
D015345:Oligonucleotide Probes; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase