title: Orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders 'Enterobacteriales' and Pasteurellales.
abstract: A novel gammaproteobacterium, strain CN3(T), was isolated from the faeces of wild boar. Strain CN3(T) was facultatively anaerobic and appeared coccoid or rod-shaped. The partial 16S rRNA gene sequence determined for strain CN3(T) suggested a distant relationship with members of the orders 'Enterobacteriales' and Pasteurellales. The gene sequence showed highest similarity (90.3 %) with Obesumbacterium proteus DSM 2777(T), a member of the family Enterobacteriaceae. The closest relatives outside the order 'Enterobacteriales' according to 16S rRNA gene sequence analysis were members of the order Pasteurellales with 88.7 % similarity (Mannheimia haemolytica NCTC 9380(T) and Actinobacillus lignieresii NCTC 4189(T)). In contrast to most members of the order 'Enterobacteriales', strain CN3(T) was oxidase-positive. The pattern of fatty acids, in particular the high relative abundance of C(18 : 1)ω7c (38.5 %), was clearly distinct from the conserved pattern found for members of the order Pasteurellales. EcoRI ribotyping of strain CN3(T) yielded no significant similarity to existing database entries. The major ubiquinone of strain CN3(T) was Q-8. The DNA G+C content was 36.4 mol%. Strain CN3(T) hosted a phage and secreted considerable amounts of three proteins into the culture supernatant. A spontaneous mutant of strain CN3(T) was isolated which formed long filaments. Microscopic studies revealed the presence of a capsule that the mutant strain was unable to partition after cell division. Strain CN3(T) thus represents a novel species within a new genus, for which the name Orbus hercynius gen. nov., sp. nov. is proposed. The type strain of the type species is CN3(T) (=DSM 22228(T)=CCUG 57622(T)). Classification of the novel species to the family and order level will require further investigations.
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Volkmann
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Skiebe
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Kerrinnes
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Faber
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Lepka
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Pfeifer
authors.affiliation: Robert Koch-Institute, ZBS 4, Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Holland
authors.affiliation: Robert Koch-Institute, ZBS 4, Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Bannert
authors.affiliation: Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Gottfried
authors.initials: G
authors.lastname: Wilharm
mesh_terms: D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D034421:Sus scrofa / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S