The helicase protein DHX29 promotes translation initiation, cell proliferation, and tumorigenesis.
Translational control plays an important role in cell growth and tumorigenesis. Cap-dependent translation initiation of mammalian mRNAs with structured 5'UTRs requires the DExH-box protein, DHX29, in vitro. Here we show that DHX29 is important for translation in vivo. Down-regulation of DHX29 leads to impaired translation, resulting in disassembly of polysomes and accumulation of mRNA-free 80S monomers. DHX29 depletion also impedes cancer cell growth in culture and in xenografts. Thus, DHX29 is a bona fide translation initiation factor that potentially can be exploited as a target to inhibit cancer cell growth.
Parsyan
Armen
A
Department of Biochemistry, Faculty of Medicine and Goodman Cancer Center, McGill University, Montreal, Quebec, Canada.
Shahbazian
David
D
Martineau
Yvan
Y
Petroulakis
Emmanuel
E
Alain
Tommy
T
Larsson
Ola
O
Mathonnet
Geraldine
G
Tettweiler
Gritta
G
Hellen
Christopher U
CU
Pestova
Tatyana V
TV
Svitkin
Yuri V
YV
Sonenberg
Nahum
N
D020121:5' Untranslated Regions; D000818:Animals; D049109:Cell Proliferation; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000201:enzymology / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D010442:Peptide Chain Initiation, Translational / Q000502:physiology*; D020365:RNA Helicases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D054682:Ribosome Subunits, Small, Eukaryotic / Q000378:metabolism; D014183:Transplantation, Heterologous
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C534219:DHX29 protein, human; D020365:RNA Helicases