title: Ki-67 proliferating index and histological grade, type and stage of colorectal carcinoma.
abstract: BACKGROUND
  The objective of this study was to assess the relationship of histological type, grade and stage of colorectal carcinoma with proliferative activity as measured by Ki-67 LI. It was a descriptive study and conducted at the Department of Pathology, University of Health Sciences, Lahore.
  
  
  METHODS
  Ki-67 is a protein associated with cell proliferation and is expressed in all the phases of cell cycle except G0. In the present study, Ki-67 expression in 50 patients with colorectal adenocarcinomas was observed using immunohistochemistry with monoclonal antibody MIB-1.
  
  
  RESULTS
  Ki-67 LI was high in well and moderately differentiated adenocarcinomas (mean Ki-67 LI 392.50 +/- 56.58 and 342.24 +/- 96.84 respectively) as compared to poorly differentiated adenocarcinomas (mean Ki-67 LI 250.00 +/- 113.46). Ki-67 LI was high in non mucinous adenocarcinomas than mucin secreting and signet ring cell adenocarcinomas (mean Ki-67 393.93 +/- 55.91 vs 220.00 +/- 49.72 and 200.00 +/- 79.05 respectively). As regard the Dukes' staging, Ki-67 LI was high in colorectal carcinomas in Dukes' stage B than tumours in Dukes' stage C (Mean Ki-67 LI 360.13 +/- 90.03 vs 241.66 +/- 101.31 respectively).
  
  
  CONCLUSIONS
  The proliferative activity as measured by Ki-67 antibody is related to histological type, grade and stage.
authors.affiliation: Department of Pathology, University of Health Sciences, Lahore. Pakistan. uzmarihan@hotmail.com
authors.forename: Uzma
authors.initials: U
authors.lastname: Nabi
authors.forename: A H
authors.initials: AH
authors.lastname: Nagi
authors.forename: Waqas
authors.initials: W
authors.lastname: Sami
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D019394:Ki-67 Antigen