title: Characteristics of biofilms from urinary tract catheters and presence of biofilm-related components in Escherichia coli.
abstract: Long term catheterization of the urinary tract leads to bacterial colonization of the urine, whereby adherence to the catheter surface is a major determinative factor for colonization. Collection of bacterial isolates from urine and urinary catheters of 45 patients showed multi-species catheter-colonization, while Escherichia coli isolates were frequently found in the urine in high numbers. Biofilm formation of catheter and urine-derived E. coli isolates was associated with the presence of the fluA gene, loss of O-antigen, and expression of type 1 fimbriae. The second messenger cyclic di-GMP (cdiGMP), a major regulator of biofilm formation, regulated adherence to the catheter surface in a selected clinical isolate suggesting that the cdiGMP second messenger pathway may be a target for anti-biofilm therapeutic approaches.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE 280, SE-171 77, Stockholm, Sweden.
authors.forename: Xiaoda
authors.initials: X
authors.lastname: Wang
authors.forename: Heinrich
authors.initials: H
authors.lastname: Lünsdorf
authors.forename: Ingrid
authors.initials: I
authors.lastname: Ehrén
authors.forename: Annelie
authors.initials: A
authors.lastname: Brauner
authors.forename: Ute
authors.initials: U
authors.lastname: Römling
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D018441:Biofilms; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D004866:Equipment Contamination; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D036781:Fimbriae Proteins / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D019081:O Antigens / Q000378:metabolism; D015290:Second Messenger Systems; D016415:Sequence Alignment; D014546:Urinary Catheterization / Q000295:instrumentation*; D014556:Urine / Q000382:microbiology
chemical_list: D018830:Adhesins, Escherichia coli; D019081:O Antigens; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP