title: Feature-level MALDI-MS characterization of in situ-synthesized peptide microarrays.
abstract: Characterizing the chemical composition of microarray features is a difficult yet important task in the production of in situ-synthesized microarrays. Here, we describe a method to determine the chemical composition of microarray features, directly on the feature. This method utilizes nondiffusional chemical cleavage from the surface along with techniques from MALDI-MS tissue imaging, thereby making the chemical characterization of high-density microarray features simple, accurate, and amenable to high-throughput.
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Greving
authors.forename: Pallav
authors.initials: P
authors.lastname: Kumar
authors.forename: Zhan-Gong
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Neal W
authors.initials: NW
authors.lastname: Woodbury
mesh_terms: D000595:Amino Acid Sequence; D004563:Electrochemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides