Deparaffinization and processing of pathologic material.
Methods for preparation of whole nuclei from paraffin embedded tissue are given. The combination of cell sorting of isolated nuclei combined with the polymerase chain reaction can be used to investigate other cellular parameters such as nuclear proteins and proliferation factors.
Cunningham
Robert E
RE
Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods*; D010336:Pathology / Q000379:methods*; D016133:Polymerase Chain Reaction
D004247:DNA