[Studies on the mutation and polymorphism of the TPMT gene in Chinese children with acute leukemia].
OBJECTIVE
To investigate the allelic frequencies and distribution of single-nucleotide polymorphisms within the coding region (cSNPs) of thiopurine S-methyltransferase gene (TPMT) in Chinese children with acute leukemia (AL) and healthy controls, in order to provide genetic references for individual chemotherapy for AL patients by studying the relationship between the cSNP in human TPMT and chemotherapeutic effect of thiopurine drugs.


METHODS
The bone marrow samples from 53 children with AL and peripheral blood samples from 115 healthy children were obtained to prepare complementary DNAs (cDNAs). The cDNAs were analyzed for the polymorphisms in the TPMT gene by reverse transcriptase-polymerase chain reaction (RT-PCR)-denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. The distribution of each genotype was evaluated.


RESULTS
Two novel heterozygote mutations, 210C>T (C70C, silent) and 622T>C (F208L), were identified in the coding region of the TPMT in a single sample, respectively. The mother of the child with mutation 622T>C was confirmed as the same genotype by DGGE and sequencing (NCBI_ss accession numbers 107796292 and 107795933). Two known polymorphisms, 474T>C (silent) and 719A>G (T240C), were identified. The allelic frequencies were 14.2%, 2.83% and 17.0%, 3.04% in the AL children and control children respectively, with the total allelic frequencies of 16.2% (first reported in the Chinese Han population) and 2.99% respectively. No association with susceptibility to disease was observed.


CONCLUSIONS
Two novel mutations and two known polymorphisms were identified in Chinese children by RT-PCR-DGGE combined with DNA sequencing, which provides the first step to identify genetic markers for predicting variability in response to and toxicity of thiopurine drugs.
Chen
Xiao-wen
XW
Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen, Guangdong, 518026 P.R. China.
Yue
Li-jie
LJ
Li
Cheng-rong
CR
Li
Chang-gang
CG
Shi
Hong-song
HS
Zhang
Min
M
D000208:Acute Disease; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002675:Child, Preschool; D005787:Gene Frequency; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D008297:Male; D008780:Methyltransferases / Q000235:genetics*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
D008780:Methyltransferases; C022745:thiopurine methyltransferase