Dual-sensitive probe 1-imidazole-2-(5-benzoacridine)-ethanone for the determination of amines in environmental water using HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometry identification.
Dual-sensitive probe of 1-imidazole-2-(5-benzoacridine)-ethanone (IBAE) for the determination of free amines with fluorescence detection and online highly sensitive atmospheric chemical ionization-mass spectrometry identification (APCI-MS) has been developed. 2-(Benzoacridine)-5-acetic acid (BAAA) reacts with coupling agent N,N'-carbonyldiimidazole (CDI) to form a highly activated amide intermediate 1-imidazole-2-(5-benzoacridine)-ethanone (IBAE), which is dual-sensitive probe. The amide intermediate (IBAE) reacts preferably with amines in dimethylformamide (DMF) solvent to give the high yields of derivatives. IBAE-amine derivatives are not only sensitive to fluorescence but also to MS ionizable efficiency. The percent ionization delta values change from 0 to 57.32% in aqueous acetonitrile and from 0 to 62.14% in aqueous methanol. The relative standard deviations of the peak areas with fluorescence detection for each amine are <1.24% (40ng/ml, n=6). The fluorescence detection limits (at a signal-to-noise ratio of 3) are in the range of 0.15-0.50ng/ml. The online APCI-MS detection limits are in the range of 2.07-8.51ng/ml (at a signal-to-noise ratio of 3). Therefore, the facile IBAE intermediate derivatization allowed the development of a highly sensitive and specific method for the quantitative analysis of trace levels of amines in environmental water.
You
Jinmao
J
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, PR China. Jmyou6304@163.com
Song
Cuihua
C
Fu
Yanyan
Y
Sun
Zhiwei
Z
Xia
Lian
L
Li
Yulin
Y
Suo
Yourui
Y
D000166:Acridines / Q000737:chemistry*; D000588:Amines / Q000032:analysis* / Q000737:chemistry; D001272:Atmosphere; D002082:Butylamines / Q000737:chemistry; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004777:Environment; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D013058:Mass Spectrometry; D009862:Online Systems; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors; D014867:Water
C547404:1-imidazole-2-(5-benzoacridine)ethanone; D000166:Acridines; D000588:Amines; D002082:Butylamines; D005456:Fluorescent Dyes; D007093:Imidazoles; D007202:Indicators and Reagents; D012997:Solvents; D014867:Water