A smart glycol-directed nanodevice from rationally designed macroporous materials.
We have developed a smart nanodevice for the highly efficient and selective detection of glycoproteins. This polyfunctional device is fabricated through the rational functionalization of macroporous silica foam (MOSF) materials with a boron species (B-MOSF) and amino groups (NH(2)-MOSF), and then the integration of MOSF, B-MOSF and NH(2)-MOSF materials. In such a device, a macroporous structure with very large-pore sizes (diameters approximately 100 nm) and high-pore volumes (>0.65 cm(3) g(-1)) is advantageous to efficiently fasten the enzymatic reaction. The targeted specific glycopeptides of the products can be selectively isolated and enriched in B-MOSF through the chemo-affinity between boronic acid and glycol groups, while the non-specific peptides are released to the solutions, or further purified by MOSF and NH(2)-MOSF, which have opposite charges. As a result, the protein digestion and glycol-peptide isolation can be simultaneously achieved in the functionalized macroporous materials in one step, which is a great advantage compared to conventional multi-procedure and time-consuming techniques.
Qian
Kun
K
Department of Chemistry and Institute of Biomedical Sciences, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, PR China.
Wan
Jingjing
J
Huang
Xiaodan
X
Yang
Pengyuan
P
Liu
Baohong
B
Yu
Chengzhong
C
D001895:Boron / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism; D006018:Glycols / Q000737:chemistry*; D006020:Glycopeptides / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
D004800:Enzymes, Immobilized; D006018:Glycols; D006020:Glycopeptides; D012822:Silicon Dioxide; D014357:Trypsin; D001895:Boron