title: Nandrolone decanoate increases satellite cell numbers in the chicken pectoralis muscle.
abstract: The anabolic androgenic steroid nandrolone decanoate has minimal androgenic effects and, thus, is widely used to induce muscle hypertrophy in both patients and athletes. Although increases in satellite cell numbers and satellite cells giving rise to new myonuclei are associated with hypertrophy in many experimental models, the relationship between nandrolone and satellite cells is poorly understood. Here we test the hypothesis that nandrolone administration is associated with an increase in satellite cell numbers in muscle. Nandrolone was injected at weekly intervals for four weeks into the right pectoralis muscle of female white leghorn chickens aged 63 days post hatch. Age/size/sex matched control birds received saline injections. The contralateral pectoralis was excised for study from each control and nandrolone treated bird. An antibody against Pax7 and immunocytochemical techniques were used to identify satellite cells. Nandrolone significantly increased mean pectoralis mass by approximately 22%, and mean fiber diameter by about 24%. All satellite cell indices that were quantified increased significantly in chicken pectoralis with administration of nandrolone. Nandrolone injected birds had on average higher satellite cell frequencies (#SC nuclei/all nuclei within basal lamina), number of satellite cells per millimeter of fiber, and satellite cell concentrations (closer together). Myonuclei were further apart (less concentrated) in nandrolone injected muscle. However, an overall increase in myonuclear numbers was revealed by a significantly greater mean number of myonuclei per millimeter of fiber in nandrolone injected muscle. Our results suggest that satellite cells may be key cellular vectors for nandrolone induced muscle fiber hypertrophy.
authors.affiliation: College of Medicine, Department of Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada.
authors.forename: Mohammed Z
authors.initials: MZ
authors.lastname: Allouh
authors.forename: Benjamin W C
authors.initials: BW
authors.lastname: Rosser
mesh_terms: D000375:Aging; D045930:Anabolic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000728:Androgens / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D048429:Cell Size; D002645:Chickens; D005260:Female; D006984:Hypertrophy; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D009277:Nandrolone / Q000008:administration & dosage / Q000494:pharmacology*; D009929:Organ Size; D051763:PAX7 Transcription Factor / Q000378:metabolism; D010369:Pectoralis Muscles / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D045930:Anabolic Agents; D000728:Androgens; D051763:PAX7 Transcription Factor; D009277:Nandrolone