title: Evaluation of human neutrophil elastase inhibitory effect of iridoid glycosides from Hedyotis diffusa.
abstract: Five iridoid glycosides were isolated from the MeOH extract of Hedyotis diffusa, and their structures were elucidated as E-6-O-p-methoxycinnamoyl scandoside methyl ester (1), Z-6-O-p-methoxycinnamoyl scandoside methyl ester (2), E-6-O-p-feruloyl scandoside methyl ester (3), E-6-O-p-coumaroyl scandoside methyl ester (4), and Z-6-O-p-coumaroyl scandoside methyl ester (5) by interpretation of their spectroscopic data. All the isolated compounds were evaluated for human neutrophil elastase inhibitory effect, and compound 1 showed potent activity with an IC(50) value of 18.0muM. The molecular docking simulation suggested a structural model for the inhibition of human neutrophil elastase by compound 1.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Xu
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Chi
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Choo
authors.forename: In-Ja
authors.initials: IJ
authors.lastname: Ryoo
authors.forename: Jong-Seog
authors.initials: JS
authors.lastname: Ahn
authors.forename: Ick-Dong
authors.initials: ID
authors.lastname: Yoo
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D032064:Hedyotis / Q000737:chemistry*; D006801:Humans; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C548714:6-O-p-methoxycinnamoyl scandoside methyl ester; D039823:Iridoids; D010936:Plant Extracts; D011480:Protease Inhibitors; D019272:Leukocyte Elastase