Reducing stress on cells with apoferritin-encapsulated platinum nanoparticles.
The great potential for medical applications of inorganic nanoparticles in living organisms is severely restricted by the concern that nanoparticles can harmfully interact with biological systems, such as lipid membranes or cell proteins. To enable an uptake of such nanoparticles by cells without harming their membranes, platinum nanoparticles were synthesized within cavities of hollow protein nanospheres (apoferritin). In vitro, the protein-platinum nanoparticles show good catalytic efficiency and long-term stability. Subsequently the particles were tested after ferritin-receptor-mediated incorporation in human intestinal Caco-2 cells. Upon externally induced stress, for example, with hydrogen peroxide, the oxygen species in the cells decreased and the viability of the cells increased.
Zhang
Lianbing
L
Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, Germany. lianbing@mpi-halle.mpg.de
Laug
Linda
L
Münchgesang
Wolfram
W
Pippel
Eckhard
E
Gösele
Ulrich
U
Brandsch
Matthias
M
Knez
Mato
M
D000975:Antioxidants / Q000737:chemistry; D001052:Apoferritins / Q000737:chemistry*; D018938:Caco-2 Cells; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D017382:Reactive Oxygen Species
D000975:Antioxidants; D011506:Proteins; D017382:Reactive Oxygen Species; D010984:Platinum; D001052:Apoferritins; D006861:Hydrogen Peroxide; D010100:Oxygen