Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori.
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
Pan
Ye
Y
Institute of Life Sciences, Jiangsu University, Zhenjiang, PR China.
Xia
Hengchuan
H
Lü
Peng
P
Chen
KePing
K
Yao
Qin
Q
Chen
Huiqin
H
Gao
Lu
L
He
Yuanqing
Y
Wang
Lin
L
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015843:Serpins / Q000737:chemistry / Q000235:genetics*; D014018:Tissue Distribution
D015843:Serpins; C434683:serpin-2