Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells.
This chapter deals with the detection of antigens that are accessible on the surface of isolated living cells using fluorochrome labels. By incubating live cells at 4 degrees C, to prevent endocytosis of bound molecules, the attached antibody can remain on the cell surface, and either be observed in the live state or subsequently fixed. This method yields the greatest sensitivity and best morphologic preservation for detection of surface molecules using antibodies for microscopy.
Willingham
Mark C
MC
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis; D005404:Fixatives; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology
D000954:Antigens, Surface; D000939:Epitopes; D005404:Fixatives; D005456:Fluorescent Dyes; D017493:Leukocyte Common Antigens