title: Endothelial cell heterogeneity of blood-brain barrier gene expression along the cerebral microvasculature.
abstract: The blood-brain barrier (BBB) refers to the network of microvessels that selectively restricts the passage of substances between the circulation and the central nervous system (CNS). This microvascular network is comprised of arterioles, capillaries and venules, yet the respective contribution of each of these to the BBB awaits clarification. In this regard, it has been postulated that brain microvascular endothelial cells (BMEC) from these different tributaries might exhibit considerable heterogeneity in form and function, with such diversity underlying unique roles in physiological and pathophysiological processes. Means to begin exploring such endothelial differences in situ, free from caveats associated with cell isolation and culturing procedures, are crucial to comprehending the nature and treatment of CNS diseases with vascular involvement. Here, the recently validated approach of immuno-laser capture microdissection (immuno-LCM) coupled to quantitative real-time PCR (qRT-PCR) was used to analyze gene expression patterns of BMEC retrieved in situ from either capillaries or venules. From profiling 87 genes known to play a role in BBB function and/or be enriched in isolated brain microvessels, results imply that most BBB properties reside in both segments, but that capillaries preferentially express some genes related to solute transport, while venules tend toward higher expression of an assortment of genes involved in inflammatory-related tasks. Fuller appreciation of such heterogeneity will be critical for efficient therapeutic targeting of the endothelium and the management of CNS disease.
authors.affiliation: Blood-Brain Barrier Laboratory, Center for Vascular Biology, University of Connecticut Health Center, Farmington, CT 07070, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Macdonald
authors.forename: Nivetha
authors.initials: N
authors.lastname: Murugesan
authors.forename: Joel S
authors.initials: JS
authors.lastname: Pachter
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000648:ultrastructure; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D002536:Cerebral Arteries / Q000502:physiology* / Q000648:ultrastructure; D002560:Cerebrovascular Circulation / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000502:physiology*; D042282:Microdissection; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014699:Venules / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D011506:Proteins; D012333:RNA, Messenger