title: The use of lentiviral vectors to obtain transgenic rats.
abstract: Lentiviral vectors are now well recognized as good vehicles for gene delivery. This is because they can efficiently transduce both dividing and post-mitotic cells, and stably integrate into the host genome allowing for long-term expression of the transgene. Their potential utility for the generation of transgenic animals has been recognized as an attractive and promising alternative to the conventional DNA-microinjection method which lacks efficiency. The initial success of lentiviral transgenesis in mice considerably broadened its use in other species, in which classical transgenic techniques are difficult, such as in the rat.In this chapter, we describe detailed procedures for both the production of human immunodeficiency virus-1 (HIV-1)-derived lentiviral vectors and for the generation of transgenic rats by injection of these vectors into the perivitelline space of fertilized one-cell eggs.
authors.affiliation: INSERM, UMR643, Nantes, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Remy
authors.forename: Tuan Huy
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D002460:Cell Line; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D008845:Microinjections / Q000379:methods; D051381:Rats; D055647:Rats, Transgenic / Q000235:genetics* / Q000821:virology; D014161:Transduction, Genetic / Q000379:methods; D014162:Transfection / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins