Automated in vitro dermal absorption (AIDA): development of a cost-effective diffusion cell.
A new diffusion cell design is reported for conducting in vitro dermal absorption tests. This disposable cell was inexpensive to construct and reduces the risk of inter-test cross-contamination. A disadvantage was that the polymer material used adsorbed the lipophilic test compound, 14C-nonyl phenol. Data is presented for absorption of tritiated water and 14C-nonyl phenol in a dialysis membrane model, Snakeskin and for tritiated water in human skin (Kp = 6.3 +/- 0.78 cm/h x 10(-3)). Further testing is needed to evaluate the capacity of the 'Dispo' cell for use with lipophilic test chemicals.
Moody
Richard P
RP
Health Canada, Environmental Health Centre, Systemic Toxicology and Pharmacokinetics Section, Ottawa, Ontario, Canada. rick_moody@hc-sc.gc.ca
Akram
Mohammed
M