title: Carbohydrate-oligonucleotide conjugates.
abstract: This unit describes a strategy for attaching two mannose and two galactose residues to an oligonucleotide. This conjugation can be performed at the 5'-end of the oligonucleotide sequence, using modified phosphoramidites. First, the oligonucleotide scaffold is synthesized on solid support using a DNA synthesizer, with commercially available and modified phosphoramidites. After the first "click" reaction with a galactosylated azide derivative on solid support, the bromine atoms are replaced with azides and a second click reaction is performed with propargylated mannose either on solid support or in solution. Additionally, using a monoalkynated solid support, the conjugation with carbohydrate residues can be performed at the 3'-end of the oligonucleotide according to a similar protocol. Curr. Protoc. Nucleic Acid Chem. 39:4.38.1-4.38.25.
authors.affiliation: Institut des Biomolécules Max Mousseron, CNRS, Université Montpellier 1, Université Montpellier 2, Montpellier, France.
authors.forename: Gwladys
authors.initials: G
authors.lastname: Pourceau
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: François
authors.initials: F
authors.lastname: Morvan
mesh_terms: D001386:Azides / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry
chemical_list: D001386:Azides; D002241:Carbohydrates; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D008358:Mannose; D005690:Galactose