Intracellular glutathione plays important roles in pyrrolizidine alkaloid clivorine-induced toxicity on L-02 hepatocytes.
ABSTRACT This study is aimed to evaluate the role of intracellular GSH in pyrrolizidine alkaloid clivorine-induced toxicity on human normal liver L-02 cells. Clivorine induced profound intracellular GSH depletion in a concentration-dependent manner. The GSH synthesis inhibitor L-Buthionine (S, R)-sulfoximine (BSO) significantly potentiated toxicity of clivorine, while GSH and GSH synthesis precursors N-Acetyl-L-cysteine (NAC) and S-adenosyl-L-methionine (SAM) protected cells against toxicity of clivorine. Overall, the results suggest that intracellular GSH plays an important role in the toxicity of clivorine on human hepatocytes.
Ji
Lili
L
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China.
Chen
Ying
Y
Wang
Zhengtao
Z