title: Liquid chromatography-atmospheric pressure photoionization tandem mass spectrometry for analysis of 36 halogenated flame retardants in fish.
abstract: A comprehensive, sensitive and high-throughput liquid chromatography-atmospheric pressure photoionization tandem mass spectrometry (LC-APPI-MS/MS) method has been developed for analysis of 36 halogenated flame retardants (HFRs). Under the optimized LC conditions, all of the HFRs eluted from the LC column within 14min, while maintaining good chromatographic separation for the isomers. Introduction of the pre-heated dopant to the APPI source decreased the background noise fivefold, which enhanced sensitivity. An empirical equation was proposed to describe the relation between the ion intensity and dopant flow. The excellent on-column instrument detection limits averaged 4.7pg, which was similar to the sensitivity offered by gas chromatography-high-resolution mass spectrometry (GC-HRMS). This method was used to analyze a series of fish samples. Good agreement was found between the results for PBDEs from LC-APPI-MS/MS and GC-HRMS.
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Avenue, St. Catharines, ON L2S 3A, Canada. simon.zhou@ontario.ca
authors.forename: Simon Ningsun
authors.initials: SN
authors.lastname: Zhou
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Reiner
authors.forename: Chris
authors.initials: C
authors.lastname: Marvin
authors.forename: Terry
authors.initials: T
authors.lastname: Kolic
authors.forename: Nicole
authors.initials: N
authors.lastname: Riddell
authors.forename: Paul
authors.initials: P
authors.lastname: Helm
authors.forename: Frank
authors.initials: F
authors.lastname: Dorman
authors.forename: Michelle
authors.initials: M
authors.lastname: Misselwitz
authors.forename: Ian D
authors.initials: ID
authors.lastname: Brindle
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005399:Fishes; D005411:Flame Retardants / Q000032:analysis*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D016014:Linear Models; D010777:Photochemistry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical