Inhibition of Streptococcus pyogenes biofilm formation by coral-associated actinomycetes.
Streptococcus pyogenes biofilms tend to exhibit significant tolerance to antimicrobials during infections. We screened coral-associated actinomycetes (CAA) for antibiofilm activity against different biofilm forming M serotype of Streptococcus pyogenes. Actinomycetes isolated from the mucus of the coral Acropora digitifera were screened for antibiofilm activity against S. pyogenes biofilms wherein several isolates clearly demonstrated antibiofilm activity. The biofilm inhibitory concentrations (BICs) and the sub-BICs (1/2 and 1/4 BIC) of the extracts significantly prevented biofilm formation up to 60-80%. The extract of Streptomyces akiyoshinensis (A3) displayed efficient antibiofilm activity against all the biofilm forming M serotypes. All the five extracts efficiently reduced the cell surface hydrophobicity (a crucial factor for biofilm formation in S. pyogenes) of three M types and thus may inhibit biofilm formation. CAA represent an interesting source of marine invertebrates-derived antibiofilm agents in the development of new strategies to combat Streptococcal biofilms.
Nithyanand
Paramasivam
P
Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Thenmozhi
Ramalingam
R
Rathna
Janarthanam
J
Pandian
Shunmugiah Karutha
SK
D039903:Actinobacteria / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D013297:Streptococcus pyogenes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D000890:Anti-Infective Agents