Patterned assembly and neurogenesis in the chick dorsal root ganglion.
The birth of small-diameter TrkA+ neurons that mediate pain and thermoreception begins approximately 24 hours after the cessation of neural crest cell migration from progenitors residing in the nascent dorsal root ganglion. Although multiple geographically distinct progenitor pools have been proposed, this study is the first to comprehensively characterize the derivation of small-diameter neurons. In the developing chick embryo we identify novel patterns in neural crest cell migration and colonization that sculpt the incipient ganglion into a postmitotic neuronal core encapsulated by a layer of proliferative progenitor cells. Furthermore, we show that this outer progenitor layer is composed of three spatially, temporally, and molecularly distinct progenitor zones, two of which give rise to distinct populations of TrkA+ neurons.
George
Lynn
L
Department of Cell Biology and Neuroscience, Montana State University, Bozeman, Montana 59717, USA. lgeorge@montana.edu
Kasemeier-Kulesa
Jennifer
J
Nelson
Branden R
BR
Koyano-Nakagawa
Naoko
N
Lefcort
Frances
F
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019521:Body Patterning / Q000502:physiology*; D002232:Carbocyanines; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D048429:Cell Size; D002642:Chick Embryo; D007839:Functional Laterality / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D056347:Neuroanatomical Tract-Tracing Techniques; D055495:Neurogenesis / Q000502:physiology*; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques; D010146:Pain / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013697:Thermosensing / Q000502:physiology
C035005:3,3'-dihexadecylindocarbocyanine; D015415:Biomarkers; D002232:Carbocyanines; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020917:Receptor, trkA