H1-antihistamines and oxidative burst of professional phagocytes.
OBJECTIVE
We analysed and compared the effect of five H1-antihistamines on stimulated oxidative burst at extra- and intracellular level of isolated and stimulated human polymorphonuclear leukocytes.


METHODS
Oxidative burst of isolated human neutrophils was studied by means of luminol and isoluminol enhanced chemiluminescence.


RESULTS
The following rank order of potency for H1-antihistamines to decrease chemiluminescence was evaluated extracellularly: dithiaden> loratadine> chlorpheniramine> brompheniramine> pheniramine and at intracellular site: loratadine> dithiaden.


CONCLUSIONS
H1-antihistamines differ substantially according to their chemical structure in suppressing oxidative burst both at extra- and intracellular site of isolated stimulated human neutrophils.
Nosal
Radomir
R
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia. exfarano@savba.sk
Drabikova
Katarina
K
Jancinova
Viera
V
Moravcova
Jana
J
Lojek
Antonin
A
Ciz
Milan
M
Macickova
Tatiana
T
Pecivova
Jana
J
D001582:Benzothiepins / Q000494:pharmacology; D001977:Brompheniramine / Q000494:pharmacology; D002744:Chlorpheniramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000187:drug effects; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects; D017336:Loratadine / Q000494:pharmacology; D049449:Luminescence; D008165:Luminol / Q000031:analogs & derivatives; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010632:Pheniramine / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*
D001582:Benzothiepins; D006634:Histamine H1 Antagonists; D010632:Pheniramine; C032487:isoluminol; D002744:Chlorpheniramine; C100170:dithiadene; D008165:Luminol; D017336:Loratadine; D001977:Brompheniramine