title: Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids.
abstract: Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM.
authors.affiliation: School of Biotechnology, Faculty of science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D001667:Binding, Competitive; D001888:Boric Acids / Q000737:chemistry / Q000494:pharmacology*; D001896:Boron Compounds / Q000737:chemistry / Q000494:pharmacology*; D001897:Boronic Acids / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008628:Mercury / Q000737:chemistry / Q000494:pharmacology; D019216:Metals, Heavy / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000201:enzymology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D012969:Sodium Fluoride / Q000737:chemistry / Q000494:pharmacology; D013025:Glycine max / Q000201:enzymology*; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D001888:Boric Acids; D001896:Boron Compounds; D001897:Boronic Acids; D004791:Enzyme Inhibitors; D019216:Metals, Heavy; D012834:Silver; C030433:n-butylboronic acid; C036647:4-bromophenylboric acid; D003300:Copper; D012969:Sodium Fluoride; D014510:Urease; D008628:Mercury; C010686:benzeneboronic acid; C032688:boric acid