Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1beta, or IFN-gamma in cultured canine keratinocytes.
Keratinocytes produce inflammatory mediators that are involved in the pathogenesis of skin disorders such as atopic dermatitis (AD). In particular, the CC chemokines, thymus and activation regulated chemokine (TARC)/CCL17 and mucosae-associated epithelial chemokine (MEC)/CCL28 are considered to play an important role in the lesional infiltration of lymphocytes in canine AD. However, there have been no reports on the regulatory mechanisms of CCL17 and CCL28 transcription in canine keratinocytes. In this study, we investigated whether CCL17 and CCL28 transcription in cultured keratinocytes is induced by TNF-alpha, IL-1beta, or IFN-gamma. It was found that CCL17 mRNA transcription is augmented by TNF-alpha only, whereas the CCL28 mRNA level could be increased by TNF-alpha, IL-1beta, or IFN-gamma. The present study suggests that pro-inflammatory cytokines are important inducing factors for the production of CCL17 and CCL28 in the lesional skin of dogs with AD.
Shibata
Sanae
S
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. k5104402@edu.gifu-u.ac.jp
Maeda
Sadatoshi
S
Maeda
Shingo
S
Chimura
Naoki
N
Kondo
Naho
N
Fukata
Tsuneo
T
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D054414:Chemokine CCL17 / Q000235:genetics*; D019742:Chemokines, CC / Q000235:genetics*; D017931:DNA Primers; D015342:DNA Probes; D003876:Dermatitis, Atopic / Q000235:genetics / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics; D004285:Dogs; D005786:Gene Expression Regulation / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D054414:Chemokine CCL17; D019742:Chemokines, CC; D017931:DNA Primers; D015342:DNA Probes; D053583:Interleukin-1beta; D012333:RNA, Messenger; D007371:Interferon-gamma