title: DD3(PCA3) gene expression in cancer and prostatic hyperplasia.
abstract: OBJECTIVE
  DD3(PCA3) is a novel gene with characteristics that indicate its potentially valuable role in early identification and diagnosis of malignancy and highly upregulated in transformed cells in PCa. The aim of this work was to validate and analyze, by real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR), the expression of the DD3(PCA3)gene in a Mexican population, both in intratumoral tissue with PCa and benign prostatic hyperplasia (BPH).
  
  
  METHODS
  Human samples from patients with PCa (40 cases) and benign prostatic hyperplasia (40 cases) were analyzed for the mRNA expression of DD3(PCA3)by RT-PCR RESULTS: The GAPDH gene showed better stability with a Pearson correlation of 0.953 (P < 0.007) for the determination of housekeeping gene. DD3(PCA3)gene expression was 29.74 times higher in PCa tissue (P < 0.0001) than in BPH. The gene expression for the PCa and BPH was 1731+/-280 and 58.23+/-9.9 fold, respectively.
  
  
  CONCLUSIONS
  Determination of DD3(PCA3)gene expression by RT-PCR could be a potentially tool for the early detection of PCa in clinical specimens.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Escuela Médico Militar, México, DF.
authors.forename: E
authors.initials: E
authors.lastname: Floriano-Sánchez
authors.forename: N
authors.initials: N
authors.lastname: Cárdenas-Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Castro-Marín
authors.forename: P
authors.initials: P
authors.lastname: Alvarez-Grave
authors.forename: E
authors.initials: E
authors.lastname: Lara-Padilla
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008297:Male; D011470:Prostatic Hyperplasia / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D017931:DNA Primers; D012333:RNA, Messenger; C479341:prostate cancer antigen 3, human