title: Phase separation of lipid microdomains controlled by polymerized lipid bilayer matrices.
abstract: We developed a micropatterned model biological membrane on a solid substrate that can induce phase separation of lipid microdomains in a designed geometry. Micropatterned lipid bilayers were generated by the photolithographic polymerization of a diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC). By changing the UV dose for the photopolymerization, we could modulate the coverage of the surface by the polymeric bilayer domains. After removing nonpolymerized DiynePC, natural phospholipid membranes were incorporated into the micropatterned polymeric bilayer matrix by a self-assembly process (vesicle fusion). As we incorporated a ternary lipid mixture of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), sphingomyelin (SM), and cholesterol (Chol) (1:1:1), liquid ordered domains (Lo: rich in SM and Chol) were accumulated in the polymer free regions, whereas liquid disordered domains (Ld: rich in DOPC) preferentially participated into the partially polymeric bilayer regions. It was postulated that Ld domains preferentially came in contact with the polymeric bilayer boundaries because of their lower elastic moduli and a smaller thickness mismatch at the boundary. The effect of polymeric bilayer matrix to hinder the size growth of Lo domains should also be playing an important role. The controlled phase separation should open new possibilities to locally concentrate membrane proteins and other nanometer-sized materials on the substrate by associating them with the lipid microdomains.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda 563-8577, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okazaki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Tatsu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Morigaki
mesh_terms: D008051:Lipid Bilayers; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids; D011108:Polymers