The differentiation and engraftment potential of mouse hematopoietic stem cells is maintained after bio-electrospray.
The bio-electrospray technique has been recently pioneered to manipulate living, immortalised and primary cells, including a wide range of stem cells. Studies have demonstrated that the creation of viable, fully functional in vitro microenvironments is possible using this technique. By modifying the bio-electrospray procedure (referred to as cell electrospinning), a variety of microenvironment morphologies have been fabricated. Because bio-electrospraying of biological material is a relatively new technique, it is important to determine if there are any unwanted consequences to the manipulated cells as a result of the procedure. Here, we establish the validity of the process using a heterogeneous, living population of hematopoietic stem/progenitor cells, using a functional in vitro assay and in vivo mouse model to investigate for side-effects that previous in vitro assays may not have detected. Our studies demonstrate that these bio-protocols have no obvious negative effects, thus indicating significant promise for utility in biological sciences and for a plethora of healthcare applications.
Bartolovic
Kerol
K
Wolfson Centre for Gene Therapy, Molecular Immunology Unit, UCL Institute of Child Health, University College London, 30 Guilford Street, London, UK.
Mongkoldhumrongkul
Napachanok
N
Waddington
Simon N
SN
Jayasinghe
Suwan N
SN
Howe
Steven J
SJ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice