title: Effects of glyoxal or methylglyoxal on the metabolism of amino acids, lactate, glucose and acetate in the cerebral cortex of young and adult rats.
abstract: The in vitro effects of glyoxal and methylglyoxal on the metabolism of glycine, alanine, leucine, glutamate, glutamine, glucose, lactate and acetate were evaluated in cortico-cerebral slices from young (10-day-old) or adult (3-month-old) rats. In a first set of experiments with cortico-cerebral slices from young animals, the compounds glyoxal or methylglyoxal at 400 microM, increased the oxidation of alanine, leucine and glycine to CO(2) and decreased the protein synthesis from these amino acids. Lipid synthesis from alanine, leucine and glycine was not changed in the cortico-cerebral slices from young rats after glyoxals exposure. Moreover, glutamine oxidation to CO(2) decreased by glyoxals exposure, but glutamate oxidation was not affected. In a second set of experiments with brain slices from adult animals, glycine metabolism (oxidation to CO(2), conversion to lipids or incorporation into proteins) was not changed by glyoxals exposure. In addition, the oxidation rates of glucose, lactate, acetate, glutamine and glutamate to CO(2) were also not modified. Taken together, these results indicate that glyoxal disrupts the energetic metabolism of the rat cerebral cortex in vitro. However, only young animals were susceptible to such events, suggesting that the immature cerebral cortex is less capable of dealing with glyoxal than the mature one.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Betina
authors.initials: B
authors.lastname: Schmidt
authors.forename: Adriano Martimbianco
authors.initials: AM
authors.lastname: de Assis
authors.forename: Cíntia Eickhoff
authors.initials: CE
authors.lastname: Battu
authors.forename: Débora Kurle
authors.initials: DK
authors.lastname: Rieger
authors.forename: Fernanda
authors.initials: F
authors.lastname: Hansen
authors.forename: Fernanda
authors.initials: F
authors.lastname: Sordi
authors.forename: Aline
authors.initials: A
authors.lastname: Longoni
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Hoefel
authors.forename: Marcelo
authors.initials: M
authors.lastname: Farina
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Diogo Onofre
authors.initials: DO
authors.lastname: Souza
authors.forename: Marcos Luiz Santos
authors.initials: ML
authors.lastname: Perry
mesh_terms: D000085:Acetates / Q000378:metabolism; D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006037:Glyoxal / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects; D011765:Pyruvaldehyde / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000085:Acetates; D000596:Amino Acids; D002491:Central Nervous System Agents; D005973:Glutamine; D002245:Carbon Dioxide; D019344:Lactic Acid; D018698:Glutamic Acid; D006037:Glyoxal; D011765:Pyruvaldehyde; D005947:Glucose; D005998:Glycine