title: Isolation, structure, and bioactivities of abiesadines A-Y, 25 new diterpenes from Abies georgei Orr.
abstract: Twenty-five new (abiesadines A-Y, 1-25) and 29 known (26-54) diterpenes were isolated from the aerial parts of Abies georgei. Abiesadine A (1) is a novel 8,14-seco-abietane, while abiesadine B (2) is a novel 9,10-seco-abietane. The structures of the new compounds were established on the basis of spectroscopic data analysis. Manool (52) showed the strongest effect against LPS-induced NO production in RAW264.7 macrophages with the IC(50) value of 11.0microg/mL. In another anti-inflammatory assay against TNFalpha-triggered NF-kappaB activity, (12R,13R)-8,12-epoxy-14-labden-13-ol (54) exhibited the strongest effect (IC(50)=8.7microg/mL). For antitumor assays, pomiferin A (26) and 8,11,13-abietatriene-7alpha,18-diol (29) both showed the most significant activity against LOVO cells (IC(50)=9.2microg/mL). While 7-oxocallitrisic acid (46) exhibited significant cytotoxicity against QGY-7703 tumor cells (IC(50)=10.2microg/mL).
authors.affiliation: Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China; Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Rd., Guangzhou 510301, PR China.
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Su-Mei
authors.initials: SM
authors.lastname: Li
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Xin-Ru
authors.initials: XR
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D028202:Abies / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D004224:Diterpenes