Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.
Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.
Kong
Weidong
W
Department of Agronomy, Purdue University, West Lafayette, IN 47907-2054, USA.
Nakatsu
Cindy H
CH
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D017753:Ecosystem; D004785:Environmental Pollutants / Q000378:metabolism; D018628:Gene Dosage; D015870:Gene Expression; D005798:Genes, Bacterial; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D004785:Environmental Pollutants; D006841:Hydrocarbons, Aromatic; D012329:RNA, Bacterial