Tissue disaggregation.
A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent.
Cunningham
Robert E
RE
Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005557:Formaldehyde; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D016707:Tissue Fixation
D005557:Formaldehyde; D010447:Peptide Hydrolases