title: In vitro detection and primary cultivation of bacteria producing materials inhibitory to ruminal methanogens.
abstract: A novel method for screening bacterial isolates for their potential to inhibit the growth of ruminal methanogenic Archaea was developed using a modification of the soft agar overlay technique, formally used for the isolation of lytic bacteriophages. This method may be used in the specific, hydrogen-rich conditions required for the growth of ruminal methanogenic Archaea.
authors.affiliation: Animal Research Institute, Queensland Primary Industries and Fisheries, Yeerongpilly, Queensland, 4105, Australia. ros.gilbert@deedi.qld.gov.au
authors.forename: Rosalind A
authors.initials: RA
authors.lastname: Gilbert
authors.forename: Diane
authors.initials: D
authors.lastname: Ouwerkerk
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Athol V
authors.initials: AV
authors.lastname: Klieve
mesh_terms: D000818:Animals; D000898:Antibiosis; D001105:Archaea / Q000254:growth & development* / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D008697:Methane / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
chemical_list: D008697:Methane