Synthesis and screening of a CaaL peptide library versus FTase reveals a surprising number of substrates.
Proteins bearing a CaaL sequence are typically geranylgeranylated to enable their proper localization and function. We found that many of the dansyl-GCaaL peptides representing mammalian CaaL proteins can be farnesylated by FTase. This result may have important implications for prenylated protein biology.
Krzysiak
Amanda J
AJ
Department of Medicinal Chemistry and Molecular Pharmacology and Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
Aditya
Animesh V
AV
Hougland
James L
JL
Fierke
Carol A
CA
Gibbs
Richard A
RA
D019883:Alkyl and Aryl Transferases / Q000378:metabolism; D000595:Amino Acid Sequence; D051231:Farnesyltranstransferase / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D054876:Prenylation; D013379:Substrate Specificity; D018631:ras Proteins / Q000378:metabolism
D019151:Peptide Library; D010455:Peptides; D019883:Alkyl and Aryl Transferases; C083291:geranylgeranyltransferase type-I; D051231:Farnesyltranstransferase; D018631:ras Proteins