title: Dynamic movements of Ro52 cytoplasmic bodies along microtubules.
abstract: The RING-finger protein Ro52/TRIM21 is known as an autoantigen and is recognized by anti-Ro/SSA antibodies, which are commonly found in patients with Sjögren's syndrome and systemic lupus erythematosus. Recently, Ro52 has been shown to localize to distinct structures called cytoplasmic bodies and function as an E3 ubiquitin ligase. However, the Ro52 cytoplasmic bodies have not been well characterized. In this study, we investigated the Ro52 cytoplasmic bodies using fluorescence microscopy. This analysis revealed that the Ro52 cytoplasmic bodies are diffusely located in the cytoplasm and exist independently of TRIM5alpha cytoplasmic bodies. Our results further showed that the Ro52 cytoplasmic bodies are not stained with MitoTracker dye and are not colocalized with the proteasome subunit Rpt5, the caveolae component caveolin-1, the endosome markers (EEA1, Rab5, and Rab7), and the lysosome marker LAMP2. These results indicate that the Ro52 cytoplasmic bodies are not mitochondria, proteasome-enriched structures, caveolae, endosomes, or lysosomes. Importantly, the Ro52 cytoplasmic bodies are highly motile and are located along the microtubule network. These results suggest that the Ro52 cytoplasmic bodies are unidentified structures that are transported along the microtubule network.
authors.affiliation: Center for Molecular Chaperone/Radiobiology and Cancer Virology, Department of Medicine, Medical College of Georgia, 1410 Laney Walker Blvd., CN-3141, Augusta, GA 30912, USA.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tanaka
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tanji
authors.forename: Motoko
authors.initials: M
authors.lastname: Niida
authors.forename: Tetsu
authors.initials: T
authors.lastname: Kamitani
mesh_terms: D002478:Cells, Cultured; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D009068:Movement; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000099207:SS-A Antigen