Use of liposomes to study vesicular transport.
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
Takei
Kohji
K
Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
Yamada
Hiroshi
H
Abe
Tadashi
T
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D002966:Clathrin / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis; D051381:Rats; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism*
D002966:Clathrin; D053835:Unilamellar Liposomes