title: Clinical relevance of the TLR4 11367 polymorphism in patients with major trauma.
abstract: OBJECTIVE
  To investigate the clinical relevance of the TLR4 11367 polymorphism in patients with major trauma.
  
  
  METHODS
  Genetic functional and association study.
  
  
  METHODS
  Daping Hospital and Chongqing Emergency Medical Center, Chongqing, China.
  
  
  METHODS
  A total of 132 patients with major trauma were prospectively recruited.
  
  
  METHODS
  The TLR4 11367 polymorphism was genotyped using single-tube, bidirectional, allele-specific amplification method. Whole peripheral blood samples obtained within 24 hours after admission were stimulated with lipopolysaccharide and then tested for production of tumor necrosis factor alpha and interleukin 6. Sepsis morbidity rate and multiple organ dysfunction scores were assessed.
  
  
  RESULTS
  The 11367 polymorphism was shown to be strongly associated with less capacity of peripheral leukocytes to produce tumor necrosis factor alpha and interleukin 6 in response to ex vivo lipopolysaccharide stimulation in patients with trauma at admission. Results from association study indicated that patients with trauma who carry the 11367C allele were less likely to have sepsis and multiple organ dysfunction.
  
  
  CONCLUSIONS
  Combined with our previous in vitro functional study, the results suggest that the TLR4 11367 polymorphism might be a good predictor of who is more likely to develop complications such as sepsis or multiple organ dysfunction syndrome, depending on genotype.
authors.affiliation: State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Zhao-xia
authors.initials: ZX
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Lian-yang
authors.initials: LY
authors.lastname: Zhang
authors.forename: Ding-yuan
authors.initials: DY
authors.lastname: Du
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Zheng-guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Hao
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D002681:China; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D018805:Sepsis / Q000453:epidemiology / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014947:Wounds and Injuries / Q000150:complications* / Q000235:genetics*; D055815:Young Adult
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4