title: Platelet-rich plasma impairs osteoclast generation from human precursors of peripheral blood.
abstract: Platelet-rich plasma is used to accelerate bone repair for the release of osteogenic growth factors from activated platelets. To date, the effects on osteoclasts have been only scarcely investigated, even though these cells are crucial for bone remodeling. The aim of this research was the evaluation of the effects of thrombin-activated platelets (PRP) on osteoclastogenesis from human blood precursors. We evaluated both the ability to influence osteoclast differentiation induced by the receptor activator of nuclear factor-kappaB ligand (RANKL), and the ability to induce osteoclast differentiation without RANKL. In both assays, the incubation with PRP supernatant at 10% did not significantly affect the formation of tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells that were able to form the F-actin ring. However, when PRP at 25 and 50% was added to the medium without RANKL, the generation of TRACP-positive multinucleated cells was inhibited. PRP, even at 10%, reduced the osteoclast-mediated bone collagen degradation, suggesting inhibition of osteoclast activation. Similarly, after incubation with PRP supernatant, calcitonin receptor mRNA was lower than the untreated samples. In conclusion, PRP at 10% interfered with the complete differentiation process of human osteoclast precursors. At higher concentration it impaired osteoclast formation also at an early stage of differentiation.
authors.affiliation: Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cenni
authors.forename: Sofia
authors.initials: S
authors.lastname: Avnet
authors.forename: Caterina
authors.initials: C
authors.lastname: Fotia
authors.forename: Manuela
authors.initials: M
authors.lastname: Salerno
authors.forename: Nicola
authors.initials: N
authors.lastname: Baldini
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000199:Actins / Q000032:analysis; D001792:Blood Platelets / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology*; D000071681:Tartrate-Resistant Acid Phosphatase; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D000199:Actins; D007527:Isoenzymes; D053245:RANK Ligand; C505688:TNFSF11 protein, human; D053773:Transforming Growth Factor beta1; D016173:Macrophage Colony-Stimulating Factor; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase