title: Anti-tobacco mosaic virus (TMV) Quassinoids from Brucea javanica (L.) Merr.
abstract: Two new quassinoids, javanicolide E (1) and javanicolide F (2), along with fifteen known C-20 quassinoids were isolated from the seeds of Brucea javanica (L.) Merr. The antitobacco mosaic virus (TMV) activity of these quassinoids was screened by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the seventeen quassinoids showed potent anti-TMV activity. Among them, eight compounds, brusatol (3), bruceine B (4), bruceoside B (5), yadanzioside I (6), yadanzioside L (7), bruceine D (8), yadanziolide A (9), and aglycone of yadanziolide D (17), showed strong antiviral activities, with IC(50) values in the range of 3.42-5.66 microM, and were much more effective than the positive control, ningnanmycin (IC(50) = 117.3 microM). The antiviral structure-activity relationships of quassinoids against TMV were also discussed.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Yan
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Ying-Tong
authors.initials: YT
authors.lastname: Di
authors.forename: Xin
authors.initials: X
authors.lastname: Fang
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Dong
authors.forename: Peng
authors.initials: P
authors.lastname: Sang
authors.forename: Yue-Hu
authors.initials: YH
authors.lastname: Wang
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: He
authors.forename: Zhong-Kai
authors.initials: ZK
authors.lastname: Zhang
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D032269:Brucea / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D036702:Quassins / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014027:Tobacco Mosaic Virus / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D036702:Quassins