title: Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline.
abstract: In the present study, we determined the antioxidant activity of cepharanthine and fangchinoline from Stephania rotunda by performing different in vitro antioxidant assays, including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, N,N- dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging, superoxide anion (O2*-) radical scavenging, hydrogen peroxide scavenging, total antioxidant activity, reducing power, and ferrous ion (Fe2+) chelating activities. Cepharanthine and fangchinoline showed 94.6 and 93.3% inhibition on lipid peroxidation of linoleic acid emulsion at 30 microg/mL concentration, respectively. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, and trolox indicated inhibitions of 83.3, 92.2, 72.4, and 81.3% on peroxidation of linoleic acid emulsion at the same concentration (30 microg/mL), respectively. According to the results, cepharanthine and fangchinoline have effective antioxidant and radical scavenging activity.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, Turkey. igulcin@atauni.edu.tr
authors.forename: Ilhami
authors.initials: I
authors.lastname: Gülçin
authors.forename: Riad
authors.initials: R
authors.lastname: Elias
authors.forename: Akçahan
authors.initials: A
authors.lastname: Gepdiremen
authors.forename: Aun
authors.initials: A
authors.lastname: Chea
authors.forename: Fevzi
authors.initials: F
authors.lastname: Topal
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D031607:Stephania / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000494:pharmacology; D052117:Benzodioxoles
chemical_list: D000975:Antioxidants; D044182:Benzylisoquinolines; D002083:Butylated Hydroxyanisole; D002084:Butylated Hydroxytoluene; D002839:Chromans; D024502:alpha-Tocopherol; C006947:cepharanthine; D052117:Benzodioxoles; C060802:fangchinoline; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid