title: Two-color, two-photon uncaging of glutamate and GABA.
abstract: We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.
authors.affiliation: Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Kantevari
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuzaki
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kanemoto
authors.forename: Haruo
authors.initials: H
authors.lastname: Kasai
authors.forename: Graham C R
authors.initials: GC
authors.lastname: Ellis-Davies
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000502:physiology*; D008029:Lighting / Q000379:methods*; D008564:Membrane Potentials / Q000502:physiology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid