Identification of NK cell receptor ligands using a signaling reporter system.
NK cell responses are regulated by a balance of inhibitory and activating signals, reflecting the net effect of interactions between receptors and ligands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in combination with a virus rescue system.
Iizuka
Yoshie-Matsubayashi
YM
Department of Medicine and Center for Immunology, Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
Somia
Nikunj V
NV
Iizuka
Koho
K
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D008024:Ligands; D051379:Mice; D011485:Protein Binding; D055607:Receptors, Natural Killer Cell / Q000235:genetics / Q000378:metabolism*
D008024:Ligands; D055607:Receptors, Natural Killer Cell