Use of the modified quick easy cheap effective rugged and safe sample preparation approach for the simultaneous analysis of type A- and B-trichothecenes in wheat flour.
A suitable extraction and purification method for the simultaneous liquid chromatography-mass spectrometry (LC-MS) determination of five mycotoxins, three type A, diacetoxyscirpenol (DAS), T-2 toxin (T-2) and HT-2 toxin (HT-2), and two type B-trichothecenes, deoxynivalenol (DON) and nivalenol (NIV), has been optimised using a modified "Quick Easy Cheap Effective Rugged and Safe" (QuEChERS) method. Different solvents were studied in the extraction procedure to obtain better recoveries, which ranged from 86 to 108%, using a 85/15 (v/v) mixture of methanol/acetonitrile. The values obtained for recovery, repeatability and reproducibility of the optimized method are in agreement with Commission Directive 2005/26/EC for methods of analysis of Fusarium toxins. Finally, this optimized procedure was applied in wheat flour samples commercialized in Spain.
Sospedra
I
I
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av.Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Blesa
J
J
Soriano
J M
JM
Mañes
J
J
D000097:Acetonitriles / Q000737:chemistry; D053000:Analytic Sample Preparation Methods / Q000191:economics*; D002853:Chromatography, Liquid; D005433:Flour / Q000032:analysis*; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D012997:Solvents; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
D000097:Acetonitriles; D012997:Solvents; D014255:Trichothecenes; D000432:Methanol; C032159:acetonitrile