Overcoming merohedral twinning in crystals of bacteriorhodopsin grown in lipidic mesophase.
Twinning is one of the most common crystal-growth defects in protein crystallography. There are neither efficient rational approaches for the growth of nontwinned protein crystals nor are there examples of systematic studies of the dependence of the twinning-ratio distribution on crystallization conditions. The description of the twinning phenomenon has been covered even less for membrane-protein crystals and is non-existent for crystals grown using lipidic phases (in meso). In the present work, possibilities for overcoming merohedral twinning are investigated for crystals of the membrane protein bacteriorhodopsin (bR) grown in meso. It is shown that traditional crystallization additives are not effective in the case of the in meso crystallization of bR. The twinning ratio was determined for 310 crystals grown under different crystallization conditions. A correlation of the twinning ratio with the growth rate of the crystals was observed. Slow growth indicated that crystals had a noticeable chance of avoiding twinning. Model calculations were performed in order to rationalize this observation. The calculations confirmed the experimental observation that most crystals consist of two twin domains and showed that under this condition small changes in the probability of twin-domain formation lead to dramatic changes in the number of nontwinned crystals, which explains why slow crystal growth results in a considerable number of nontwinned crystals.
Borshchevskiy
Valentin
V
Laboratoire des Protéines Membranaires, Institute de Biologie Structurale J.-P. Ebel, UMR5075 CEA-CNRS-UJF, Grenoble 38027, France.
Efremov
Rouslan
R
Moiseeva
Ekaterina
E
Büldt
Georg
G
Gordeliy
Valentin
V
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D019617:Halobacterium salinarum; D008055:Lipids / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D018717:Purple Membrane / Q000378:metabolism
D008055:Lipids; D001436:Bacteriorhodopsins