Properties and antityrosinase activity of sericin from various extraction methods.
The present study investigated the chemical properties and antityrosinase activities of SS (silk sericin) extracted from different Thai silk strains via various extraction methods. Different silk strains contain distinct SS with various amino acid compositions, which are significantly influenced by the extraction method used. Urea extraction of SS was the only method that provided clearly distinguishable bands and had the most significant impact on SS conformation as illustrated by FTIR (Fourier-transform infrared) spectra. The use of urea or either acidic or alkaline chemicals in the extraction process also influenced SS thermal behaviour. With regard to biological activity, SS extracted using urea exhibited the highest antityrosinase activity, whereas alkali-degraded SS showed no inhibition of mushroom tyrosinase. Pigments, primarily flavonoids and carotenoids from silk cocoons, were also found to enhance tyrosinase inhibition of SS.
Aramwit
Pornanong
P
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. aramwit@gmail.com
Damrongsakkul
Siriporn
S
Kanokpanont
Sorada
S
Srichana
Teerapol
T
D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry / Q000302:isolation & purification; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000737:chemistry; D047030:Sericins / Q000737:chemistry / Q000302:isolation & purification; D014508:Urea / Q000737:chemistry
D005656:Fungal Proteins; D019476:Insect Proteins; D047030:Sericins; D014508:Urea; D014442:Monophenol Monooxygenase