[Chromosomal localization and development of SNP markers of a serine protease gene in Farrer's scallop (Chlamys farreri)].
Disease control is a main objective in the culture of Farrer's scallop (Chalmys farreri). Many investigations have focused on the innate immune system of this species. Serine proteases amplify signals in many immune-related pathways and thus play a crucial role in innate immune system. Recently, studies on serine protease in C. farreri were mainly on sequence analysis and expression profile, but no study on gene localization was reported. In this study, a BAC clone (CFB040H03) that contains a serine protease gene of C. farreri was located on the long arms of a pair of homologous chromosomes by BAC-FISH technique. Six SNPs were found inside the serine protease gene using direct sequencing of the PCR products. The chromosomal localization of serine protease gene would provide fundamental support for further research on this gene. The SNPs obtained can be mapped to the genetic linkage map and is helpful to the construction of the integration map of C. farreri.
Huan
Pin
P
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. huanpin@ms.qdio.ac.cn
Zhang
Xiao-Jun
XJ
Zhang
Xiao-Jun
XJ
Li
Fu-Hua
FH
Zhang
Yang
Y
Zhao
Cui
C
Liu
Bao-Zhong
BZ
Xiang
Jian-Hai
JH
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000201:enzymology* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D057057:Serine Proteases / Q000235:genetics* / Q000378:metabolism
D057057:Serine Proteases