title: [Determination of trace free copper and zinc in serum samples by graphite furnace atomic absorption spectrometry after two-step precipitation of protein].
abstract: A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1 : 2. The mixture was subsequently denatured at 70 degrees C and centrifuged to precipitate proteins. The determination, limit of copper (3sigma) was 1.2 microg x L(-1) and the recovery was 92.3% - 104%, and the determination. Limit of zinc(3sigma) was 0.098 microg x L(-1) and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people.
authors.affiliation: Central Laboratories, Shantou University Medical College, Shantou 515041, China. yzhang@stu.edu.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhe-Xuan
authors.initials: ZX
authors.lastname: Lin
authors.forename: Wei-Qiu
authors.initials: WQ
authors.lastname: Li
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Luo
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Luo
mesh_terms: D003300:Copper / Q000032:analysis*; D006108:Graphite; D006801:Humans; D009369:Neoplasms / Q000737:chemistry; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000032:analysis*
chemical_list: D006108:Graphite; D003300:Copper; D015032:Zinc