Effect of carbon dioxide on broth microdilution susceptibility testing of Brucella spp.
Since some strains of Brucella species may require carbon dioxide for growth, a multilaboratory study was conducted to compare broth microdilution susceptibility results using ambient air (AA) and 5% CO2 incubation conditions. Six antimicrobial agents were tested against 39 Brucella isolates. Aminoglycoside MICs tended to be 1 log2 dilution higher in CO2 than in AA; tetracycline-class MICs to be 1 log2 dilution lower in CO2.
Lonsway
David R
DR
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. dul7@cdc.gov
Jevitt
Laura A
LA
Uhl
James R
JR
Cockerill
Franklin R
FR
Anderson
Mary E
ME
Sullivan
Maureen M
MM
De
Barun K
BK
Edwards
Jonathan R
JR
Patel
Jean B
JB
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002002:Brucella / Q000187:drug effects* / Q000254:growth & development; D002245:Carbon Dioxide / Q000378:metabolism*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods
D000900:Anti-Bacterial Agents; D002245:Carbon Dioxide