Ezrin is a key molecule in the metastasis of MOLT4 cells induced by CCL25/CCR9.
Chemokines and their corresponding receptors participate in transmigration of leukemic cells. ERM (ezrin/radixin/moesin) protein family act as linkers between the plasma membrane and the cytoskeleton and are key component in tumor metastasis. In this study we used the CCR9-expressing acute T lymphocytic cell line MOLT4 as a model and assesssed their morphological and functional changes in response to CCL25. The pseudopodium formed and ERM translocated from the cytoplasm to the cell membrane following treatment with CCL25. Ezrin silencing by miRNA showed that ezrin plays important roles in the polarization and invasive behaviour of MOLT4 cells.
Zhou
Beibei
B
Department of Immunology, College of Basic Medical Science, Wuhan University, Wuhan, Hubei, China.
Leng
Jun
J
Hu
Meng
M
Zhang
Li
L
Wang
Zhan
Z
Liu
Dongying
D
Tong
Xiaoling
X
Yu
Beibei
B
Hu
Yi
Y
Deng
Chaohua
C
Liu
Yanping
Y
Zhang
Qiuping
Q
D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects / Q000648:ultrastructure; D016764:Cell Polarity / Q000187:drug effects / Q000235:genetics; D019742:Chemokines, CC / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008871:Microvilli / Q000235:genetics / Q000378:metabolism; D009362:Neoplasm Metastasis; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D034741:RNA, Small Interfering / Q000494:pharmacology; D054388:Receptors, CCR / Q000187:drug effects*; D019311:Staurosporine / Q000494:pharmacology; D000099149:Ezrin
D015820:Cadherins; D019742:Chemokines, CC; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; D054388:Receptors, CCR; D019311:Staurosporine; D000099149:Ezrin; C119309:CC chemokine receptor 9; C108074:CCL25 protein, human; C071058:moesin; C071776:radixin