Interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma.
The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line (Mz-ChA-1) were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.
Wang
Xin
X
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. newman3008@hotmail.com
Huang
Kai
K
Xu
Lining
L
D045744:Cell Line, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D005706:Gallbladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D056284:Histone Deacetylase 1 / Q000378:metabolism*; D006801:Humans; D054730:Protein Interaction Domains and Motifs; D016160:Retinoblastoma Protein / Q000235:genetics*; D015398:Signal Transduction
D019941:Cyclin-Dependent Kinase Inhibitor p16; D050687:E2F1 Transcription Factor; D016160:Retinoblastoma Protein; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1