Graphene oxide: a substrate for optimizing preparations of frozen-hydrated samples.
Graphene oxide is a hydrophilic derivative of graphene to which biological macromolecules readily attach, with properties superior to those of amorphous carbon films commonly used in electron microscopy. The single-layered crystalline lattice of carbon is highly electron transparent, and exhibits conductivity higher than amorphous carbon. Hence, graphene oxide is a particularly promising substrate for the examination of biological materials by electron microscopy. In this manuscript we compare graphene oxide films to commonly used amorphous carbon films, describing the use of graphene in optimizing the preparation of unstained, vitrified biological macromolecules.
Pantelic
Radosav S
RS
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Martinsried, Germany.
Meyer
Jannik C
JC
Kaiser
Ute
U
Baumeister
Wolfgang
W
Plitzko
Jürgen M
JM
D006108:Graphite / Q000737:chemistry*; D016591:Histocytological Preparation Techniques / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D010087:Oxides / Q000737:chemistry*
D010087:Oxides; D006108:Graphite