title: Effects of acrylamide on the activity and structure of human brain creatine kinase.
abstract: Acrylamide is widely used worldwide in industry and it can also be produced by the cooking and processing of foods. It is harmful to human beings, and human brain CK (HBCK) has been proposed to be one of the important targets of acrylamide. In this research, we studied the effects of acrylamide on HBCK activity, structure and the potential binding sites. Compared to CKs from rabbit, HBCK was fully inactivated at several-fold lower concentrations of acrylamide, and exhibited distinct properties upon acrylamide-induced inactivation and structural changes. The binding sites of acrylamide were located at the cleft between the N- and C-terminal domains of CK, and Glu232 was one of the key binding residues. The effects of acrylamide on CK were proposed to be isoenzyme- and species-specific, and the underlying molecular mechanisms were discussed.
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sheng
authors.affiliation: Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314050, China.|State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: He-Chang
authors.initials: HC
authors.lastname: Zou
authors.affiliation: Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Lü
authors.affiliation: Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.affiliation: Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314050, China.|Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yong-Doo
authors.initials: YD
authors.lastname: Park
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Yan
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Shan-Jing
authors.initials: SJ
authors.lastname: Yao
mesh_terms: D020106:Acrylamide / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D052276:Creatine Kinase, BB Form / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D062105:Molecular Docking Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011817:Rabbits
chemical_list: D020106:Acrylamide; D052276:Creatine Kinase, BB Form