Functional analysis of human NK cells by flow cytometry.
Natural killer (NK) cells are a subset of lymphocytes that contribute to innate immunity through cytokine secretion and target cell lysis. NK cell function is regulated by a multiplicity of activating and inhibitory receptors. The advance in instrumentation for multi-color flow cytometry and the generation of specific mAbs for different epitopes related to phenotypic and functional parameters have facilitated our understanding of NK cell responses. Here, we provide protocols for flow cytometric evaluation of degranulation and cytokine production by human NK cells from peripheral blood at the single-cell level. In addition to offering insight into the regulation of human NK cell responses, these techniques are applicable to the assessment of various clinical conditions, including the diagnosis of immunodeficiency syndromes.
Bryceson
Yenan T
YT
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Fauriat
Cyril
C
Nunes
João M
JM
Wood
Stephanie M
SM
Björkström
Niklas K
NK
Long
Eric O
EO
Ljunggren
Hans-Gustaf
HG
D000818:Animals; D015550:Cell Degranulation; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D016207:Cytokines