title: Prostate stem cell antigen mRNA in peripheral blood as a potential predictor of biochemical recurrence in high-risk prostate cancer.
abstract: OBJECTIVE
  To determine whether the presence of prostate stem cell antigen (PSCA) mRNA in peripheral blood can predict biochemical recurrence (BCR) after radical prostatectomy in patients with high-risk prostate cancer.
  
  
  METHODS
  High-risk disease was defined based on the presence of any one of the following three risk factors: prostate-specific antigen (PSA) > or = 20 ng/ml, a biopsy Gleason score (GS) > or = 8, or clinical stage > or = T2c. Nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect PSCA mRNA-bearing cells in peripheral blood. The relationship between PSCA detection and BCR after surgery was evaluated.
  
  
  RESULTS
  Of the 250 patients, 103 (36.8%) with high-risk disease were included in the analysis. PSCA-mRNA was detected in 17 (16.5%) of these patients. Patients with high GS (> or = 7) tended to be PSCA-mRNA positive status (P = 0.045). Over a median 23 months of follow-up (range 3-47 months), BCR developed in 27 patients (26.2%). Cox regression hazards model analysis revealed that a RT-PCR PSCA positivity (HR, 4.549; 95%CI, 1.685-12.279; P = 0.003) independently increased the risk of BCR.
  
  
  CONCLUSIONS
  The presence of PSCA mRNA in peripheral blood is a significant predictor of BCR after radical prostatectomy in high-risk prostate cancer.
authors.affiliation: Urologic Oncology Clinic, Center for Specific Organs Cancer, National Cancer Center, Goyang, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Joung
authors.forename: Kang Su
authors.initials: KS
authors.lastname: Cho
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Ho Kyung
authors.initials: HK
authors.lastname: Seo
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Chung
authors.forename: Weon Seo
authors.initials: WS
authors.lastname: Park
authors.forename: Moon Kyong
authors.initials: MK
authors.lastname: Choi
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D009364:Neoplasm Recurrence, Local / Q000097:blood* / Q000175:diagnosis; D011237:Predictive Value of Tests; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D012333:RNA, Messenger; D012307:Risk Factors
chemical_list: D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C110843:PSCA protein, human; D012333:RNA, Messenger