Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions.
Frankia is an actinobacterium that fixes nitrogen under both symbiotic and free-living conditions. We identified genes upregulated in free-living nitrogen-fixing cells by using suppression subtractive hybridization. They included genes with predicted functions related to nitrogen fixation, as well as with unknown function. Their upregulation was a novel finding in Frankia.
Yamaura
Masatoshi
M
Graduate School of Science and Engineering, Kagoshima University, Korimoto 1-21-35, Kagoshima 890-0065, Japan.
Uchiumi
Toshiki
T
Higashi
Shiro
S
Abe
Mikiko
M
Kucho
Ken-Ichi
K
D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D040161:Frankia / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D005798:Genes, Bacterial; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015854:Up-Regulation
D004269:DNA, Bacterial