[Establishment of reference sequences of hepatitis B virus genotype B and C in China].
OBJECTIVE
To establish the reference sequences of genotype B and C of hepatitis B virus in China.


METHODS
Genome sequences of Hepatitis B virus isolated from different area in China were retrieved from GenBank. These genome sequences were alignmented and the most common sequences were regarded as reference sequences. The amino acid sequences were also alignmented.


RESULTS
The homology was 99.32% between Bc genome and Ba genome, and it was 95.52% between Bc genome and Bj genome. The S gene sequence homology was 99.71% between Bc and Ba, and it was 98.68% between Bc and Bj. The homology was 98.44% between Cc genome and C genome, and it was 93.97% between Cc genome and Caus genome. The S gene sequence homology was 99.27% between Cc and C, and it was 95.01% between Cc and Caus. There was significant difference at the sites of 1762, 1764, 1858 between genotype B and genotype C (P < 0.05). The amino acid sequences were also different between genotype B and genotype C.


CONCLUSIONS
Bc and Cc sequences of Hepatitis B virus can be regarded as the reference sequences of genotype B and C.
Zhang
Zhen-hua
ZH
Department of Infectious Diseases, the First Affiliated Hospital, Anhui Medical University, Hefei 230022, China. zzh1974cn@yahoo.com.cn
Zhang
Ling
L
Lu
Meng-ji
MJ
Yang
Dong-liang
DL
Li
Xu
X
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012015:Reference Standards; D016415:Sequence Alignment; D017385:Sequence Homology
D017931:DNA Primers