Identification and analysis of novel transcripts and promoters in the human killer cell immunoglobulin-like receptor (KIR) genes.
This chapter describes the techniques our lab has used to find the multiple promoters present in individual KIR genes. Our previous studies in the murine Ly49 gene family led us to expect the presence of distal promoters, antisense transcripts, and bi-directional promoters in the KIR gene cluster. We present here all of the techniques used to systematically determine if a gene possesses these types of control elements.
Li
Hongchuan
H
Cancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, MD, USA.
Wright
Paul W
PW
Anderson
Stephen K
SK
D000818:Animals; D002457:Cell Extracts / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D018274:Electroporation; D017930:Genes, Reporter / Q000235:genetics; D005821:Genetic Techniques; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016372:RNA, Antisense / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D054340:Receptors, KIR / Q000235:genetics*; D048348:Reverse Transcription; D012260:Ribonucleases / Q000378:metabolism; D013696:Temperature; D024363:Transcription Initiation Site; D014162:Transfection
D002457:Cell Extracts; D016372:RNA, Antisense; D012333:RNA, Messenger; D054340:Receptors, KIR; D008156:Luciferases; D012260:Ribonucleases