[RAPD analysis and construction of specific DNA probes between Pinellia ternata and Typhonium flagelliforme].
OBJECTIVE
To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme.


METHODS
Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes and then conducted Southern blot.


RESULTS
A great deal of special bands was found between Pinellia ternata and Typhonium flagelliforme. A Pinellia ternata specific molecule was screened.


CONCLUSIONS
RAPD analysis and specific DNA probes show potential value in the identification of Pinellia ternata and Typhonium flagelliforme.
Chen
Guo
G
Institute of Immunology & Molecular Biology, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
Bai
Quan
Q
Wang
Chao-li
CL
Ren
Bi-xuan
BX
Tang
En-jie
EJ
Feng
Li
L
Jing
Bao-qian
BQ
Hu
Wei-min
WM
D002681:China; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D030021:Pinellia / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D015342:DNA Probes; D018744:DNA, Plant