Colorimetric sensing of alpha-amino acids and its application for the "label-free" detection of protease.
A new indirect approach to explore sensitive colorimetric sensors toward alpha-amino acids is proposed: the pink solution of 1 and copper ions changed to colorless immediately upon the addition of alpha-amino acids. As the hydrolysis of bovine serum albumin (BSA) with the aid of trypsin produces alpha-amino acids, the complex of 1/Cu(2+)/BSA could act as a label-free, sensitive, selective sensor toward trypsin. The detection process could be visually observed by naked eyes.
Lou
Xiaoding
X
Department of Chemistry, Hubei Key Lab on Organic and Polymeric Opto-Electronic Materials, Wuhan University, Wuhan 430072, China.
Zhang
Liyao
L
Qin
Jingui
J
Li
Zhen
Z
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002151:Calorimetry; D002417:Cattle; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D012235:Rhodamines; D012710:Serum Albumin, Bovine; D003300:Copper; D010447:Peptide Hydrolases; C029773:rhodamine B