title: Extraction of Teucrium manghuaense and evaluation of the bioactivity of its extract.
abstract: The ethanol extract of Teucrium manghuaense grown in Hainan province (China) was analysed by GC and GC/MS. Of the constituents 84-96% were identified on the basis of their GC retention times and their mass spectra in regard to authentic compounds. The results revealed that it contained 9-methyl-9-azabicyclo[4.2.1]nonane (7.43%), 2-methylpyrrolidine (19.42%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10.84%), and squalene (28.55%), as major components, constituting 66.24% of the extract. The chemical characterization of the ethanol extract by GC/MS also allowed identification of 1-octen-3-one (3.41%), 2-pentyl-piperidine (2.25%), 1-(2-methyl-1-propenyl)-piperidine (4.63%), 2,2'-diethoxy-5,5'-bi-1-pyrroline (0.41%), (Z,Z,Z)-9,12,15-octadecatrieniic acid, 2,3-dihydroxypropyl ester (1.56%), vitamin E (2.95%) and stigmasterol (1.39%). Finally, the antioxidant and anti-tumor activities of the ethanol extract have been evaluated. Results show that the extract of Teucrium manghuaense leaf possesses strong DPPH(-), hydroxyl radical scavenging activity and anti-tumor activity.
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, China. hn.yingh76@yahoo.com.cn <hn.yingh76@yahoo.com.cn>
authors.forename: Guihao
authors.initials: G
authors.lastname: Yin
authors.forename: Huicai
authors.initials: H
authors.lastname: Zeng
authors.forename: Mingjun
authors.initials: M
authors.lastname: He
authors.forename: Mingyue
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D031367:Teucrium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D000431:Ethanol