Cloning and characterization of three novel WSSV recognizing lectins from shrimp Marsupenaeus japonicus.
C-type lectins (CTLs) acting as pattern recognition receptors play essential roles in shrimp innate immune responses. Using WSSV envelope proteins (VP26, VP28, and VP281) to screen a phage display library of Marsupenaeus japonicus, three lectins (termed as MjLecA, MjLecB, and MjLecC) were found to interact with WSSV. Sequence analysis revealed that these MjLecs shared low similarities with each other. Phylogenetic analysis indicated MjLecA and MjLecB are likely to belong to the same lectin sub-family, while MjLecC belongs to another sub-family. These MjLecs showed broad, unique carbohydrate binding spectra. Also, the three MjLecs could interact with several envelope proteins of WSSV and could recognize a wide range of microorganisms. Moreover, binding of MjLecA or MjLecB to WSSV reduced the viral infection rate in vitro. These results suggest that various kinds of CTLs with structural and functional diversities may constitute a recognizing network against invading pathogens such as bacteria and virus, and play essential roles in the defence system of shrimp.
Song
Kang-Kang
KK
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 184# DaXue Road, Xiamen 361005, PR China.
Li
Deng-Feng
DF
Zhang
Ming-Chang
MC
Yang
Hai-Jie
HJ
Ruan
Ling-Wei
LW
Xu
Xun
X
D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D017123:Bacteriophage T7 / Q000235:genetics; D050260:Carbohydrate Metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism / Q000821:virology; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D010802:Phylogeny; D010843:Pichia / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000378:metabolism*
D037102:Lectins; D011994:Recombinant Proteins