title: The effect of phytohormones on safflower regeneration plant.
abstract: OBJECTIVE
  To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation.
  
  
  METHODS
  The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones.
  
  
  RESULTS
  1. Different hormones by single using induced calli, but didn't directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium: MS + 3% Sucrose + 0.7% Agar + 1.0 mg/L NAA; differentiation medium: MS + 3% Sucrose + 0.7% Agar + 0.2 mg/L NAA + 1.0 mg/L 6-BA; rooting medium: 1/4 MS + 2.0 mg/L NAA + 0.5 mg/L IAA.
  
  
  CONCLUSIONS
  We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.
authors.affiliation: Ministry of Education Bioreactor and Drug Development Research Center, Jilin Agricultural University, Changchun 130118, China. xiziyang512@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Li-dong
authors.initials: LD
authors.lastname: Xiong
authors.forename: Tian-hang
authors.initials: TH
authors.lastname: Li
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Qu
authors.forename: Hong-qi
authors.initials: HQ
authors.lastname: Fu
authors.forename: Xiu-ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Xiao-kun
authors.initials: XK
authors.lastname: Li
mesh_terms: D031187:Carthamus tinctorius / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators