Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses.
The 2009 pandemic influenza A (H1N1) virus exhibits hemagglutinin protein sequence homology with the 1918 pandemic influenza virus. We found that human monoclonal antibodies recognized the Sa antigenic site on the head domains of both 1918 and 2009 hemagglutinins, a site that is hypervariable due to immune selection. These antibodies exhibited high potency against the 2009 virus in vitro, and one exerted a marked therapeutic effect in vivo.
Krause
Jens C
JC
Departments of Pediatrics and of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.
Tumpey
Terrence M
TM
Huffman
Chelsey J
CJ
McGraw
Patricia A
PA
Pearce
Melissa B
MB
Tsibane
Tshidi
T
Hai
Rong
R
Basler
Christopher F
CF
Crowe
James E
JE
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004196:Disease Outbreaks; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000453:epidemiology / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000276:immunology; D016415:Sequence Alignment; D013552:Swine
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus