title: Enantiomeric separation of chiral polycyclic musks by capillary electrophoresis: Application to the analysis of cosmetic samples.
abstract: The enantiomeric separation of four chiral polycyclic musks (Galaxolide, Tonalide, Traseolide and Phantolide) using CE was achieved for the first time in this work. Two chiral methodologies were developed by CD-MEKC using SDS as surfactant in a CHES buffer (pH 9.0). One methodology enabled the fast enantiomeric separation of individual polycyclic musks with analysis times lower than 10min for Tonalide, 13min for Traseolide and Phantolide, and 17min for Galaxolide. Enantiomeric resolutions obtained were higher than 1.5 using different separation media for each compound. A second methodology was also developed enabling the simultaneous enantioseparation of the four musks. In this case, the use of a dual CD system containing two neutral CDs was necessary to achieve the separation of all enantiomers from three out of four musks in 45min. Although a coelution between Galaxolide and Phantolide was observed, the use of different UV absorption wavelengths allowed the simultaneous analysis of both musks. In addition, a sweeping strategy was performed in order to increase the sensitivity of the method. Appropriate analytical characteristics (linearity, LOD and LOQ, precision and absence of matrix interferences) were obtained for conventional and sweeping methodologies. Finally, the usefulness of the method was demonstrated in the determination of the enantiomers of the polycyclic musks in personal care products as perfumes.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Martínez-Girón
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Crego
authors.forename: Maria José
authors.initials: MJ
authors.lastname: González
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Marina
mesh_terms: D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D007189:Indans / Q000737:chemistry / Q000302:isolation & purification; D016014:Linear Models; D010476:Perfume / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013764:Tetrahydronaphthalenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: C110695:5-acetyl-1,1,2,6-tetramethyl-3-isopropylindane; D001578:Benzopyrans; D003505:Cyclodextrins; D007189:Indans; D010476:Perfume; D011083:Polycyclic Compounds; D013764:Tetrahydronaphthalenes; C033119:galaxolide; C033117:phantolid; C033116:acetyl methyl tetramethyl tetralin