Quantitative expression analysis of meristem identity genes in Eucalyptus occidentalis: AP1 is an expression marker for flowering.
A number of Eucalyptus species exhibit precocious flowering, flowering within a year of germination and often while still exhibiting juvenile foliage. To understand the nature of precocious flowering in Eucalyptus occidentalis, partial homologues of the inflorescence meristem identity gene TERMINAL FLOWER1 and of the floral meristem identity genes LEAFY and APETALA1 (EOTFL1, EOLFY and EOAP1, respectively) were isolated and characterized. The expression patterns of these meristem identity genes during the development of branched and single-stem plants were analysed by quantitative reverse transcriptase PCR. All E. occidentalis plants commenced flowering within 40 weeks of germination. However, the branched plants reached maximum flowering some 5-6 weeks earlier than did single-stem plants. Levels of EOTFL1 and EOLFY expression varied little during the study period irrespective of architecture treatment, whereas expression of EOAP1 reached a peak coincident with peak flowering in both branched and single-stem plants. AP1 is clearly an expression marker for flowering in this species.
Jaya
Elizabeth S K D
ES
Institute of Molecular Biosciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Clemens
John
J
Song
Jiancheng
J
Zhang
Huaibi
H
Jameson
Paula E
PE
D015139:Blotting, Southern; D005052:Eucalyptus / Q000235:genetics* / Q000502:physiology; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018519:Meristem / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D010940:Plant Proteins; D018749:RNA, Plant