title: [Extracts of mucopolysaccharides from Asterias rollestoni and its immunoregulation effect in vitro].
abstract: OBJECTIVE
  To study immunoregulation effects on normal pulpal cells of the mucopolysaccharides MP1 and MP2 extracted from Asterias rollestoni body wall and viscera, respectively.
  
  
  METHODS
  The orthogonal design was employed to obtain the best possible combination of the critical parameters for mucopolysaccharide MP2. MP1, MP2, glycosides, MP1 + glycosides and MP2 + glycosides were added respectively into pulpal cells culture media to test their effects on spleen cells proliferations by MTT assay.
  
  
  RESULTS
  MP1 and MP2 could certainly increase the proliferation of the normal splenocyte and inhibit glycosides cytotoxicity. Compared with controls, MP2 reversed the cytotoxicity of glycosides at concentration of 25 to 40 mg/mL, the greatest activity appearing at the dose of 40 mg/mL (P<0.01), while MP1 didn't reverse it.
  
  
  CONCLUSIONS
  MP1 and MP2 can enhance immunity of normal mice, and MP2 can significantly reverses the cytotoxicity of glycosides in vitro.
authors.affiliation: Marine College of Shandong University in Weihai, Weihai 264209, China. xfl_19@yahoo.cn
authors.forename: Fa-long
authors.initials: FL
authors.lastname: Xu
authors.forename: Yu-qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Fei-fei
authors.initials: FF
authors.lastname: Li
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Shen-xi
authors.initials: SX
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D047271:Asterias / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006025:Glycosaminoglycans / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000008:administration & dosage / Q000633:toxicity; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D006025:Glycosaminoglycans; D012503:Saponins