title: Comparison of fluorescence in situ hybridisation using peptide nucleic acid probes, Gram stain/acridine orange leukocyte cytospin and differential time to positivity methods for detection of catheter-related bloodstream infection in patients after haematopoietic stem cell transplantation.
abstract: In 46 febrile neutropenic patients who had undergone haematopoietic stem cell transplantation, the fluorescence in situ hybridisation using peptide nucleic acid probes (PNA FISH), Gram stain/acridine orange leukocyte cytospin (Gram/AOLC), and differential time to positivity (DTP) methods were performed for detection of catheter-related bloodstream infections (CRBSIs). As compared with the DTP method (which detected 11 patients with CRBSI), the PNA FISH and the Gram/AOLC methods detected ten of 11 CRBSI patients, resulting in a sensitivity, specificity, negative predictive value and positive predictive value of 91%, 100%, 97% and 100%, respectively, for the PNA FISH method as well as for the Gram/AOLC method.
authors.affiliation: Department of Internal Medicine, Division of Pulmonology, Medical University of Graz, Graz, Austria. robert.krause@medunigraz.at
authors.forename: R
authors.initials: R
authors.lastname: Krause
authors.forename: H F
authors.initials: HF
authors.lastname: Salzer
authors.forename: M
authors.initials: M
authors.lastname: Hönigl
authors.forename: T
authors.initials: T
authors.lastname: Valentin
authors.forename: H W
authors.initials: HW
authors.lastname: Auner
authors.forename: I
authors.initials: I
authors.lastname: Zollner-Schwetz
mesh_terms: D000328:Adult; D000368:Aged; D055499:Catheter-Related Infections / Q000175:diagnosis*; D005260:Female; D005335:Fever of Unknown Origin / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects*