title: pH effects on the hyaluronan hydrolysis catalysed by hyaluronidase in the presence of proteins: Part I. Dual aspect of the pH-dependence.
abstract: Hyaluronan (HA) hydrolysis catalysed by hyaluronidase (HAase) is strongly inhibited when performed at a low ratio of HAase to HA concentrations and at low ionic strength. This is because long HA chains can form non-active complexes with HAase. Bovine serum albumin (BSA) is able to compete with HAase to form electrostatic complexes with HA so freeing HAase which then recovers its catalytic activity. This BSA-dependence is characterised by two main domains separated by the optimal BSA concentration: below this concentration the HAase activity increases when the BSA concentration is increased, above this concentration the HAase activity decreases. This occurs provided that HA is negatively charged and BSA is positively charged, i.e. in a pH range from 3 to 5.25. The higher the pH value the higher the optimal BSA concentration. Other proteins can also modulate HAase activity. Lysozyme, which has a pI higher than that of BSA, is also able to compete with HAase to form electrostatic complexes with HA and liberate HAase. This occurs over a wider pH range that extends from 3 to 9. These results mean that HAase can form complexes with HA and recover its enzymatic activity at pH as high as 9, consistent with HAase having either a high pI value or positively charged patches on its surface at high pH. Finally, the pH-dependence of HAase activity, which results from the influence of pH on both the intrinsic HAase activity and the formation of complexes between HAase and HA, shows a maximum at pH 4 and a significant activity up to pH 9.
authors.affiliation: Laboratoire Polymères, Biopolymères, Surfaces, FRE 3101 CNRS - Université de Rouen, 76821 Mont-Saint-Aignan cedex, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Lenormand
authors.forename: Brigitte
authors.initials: B
authors.lastname: Deschrevel
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Vincent
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D009113:Muramidase / Q000378:metabolism; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: D011506:Proteins; D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid; D009113:Muramidase; D006821:Hyaluronoglucosaminidase