title: emPAI Calc--for the estimation of protein abundance from large-scale identification data by liquid chromatography-tandem mass spectrometry.
abstract: emPAI Calc is an open-source web application for the estimation of protein abundance. It uses the correlation between the number of identified peptides and protein abundance in mass spectrometry-based proteomic experiments. The program is the first implementation of our previously reported emPAI algorithm; it calculates the emPAI from the protein identification results obtained by database search engines such as Mascot.
authors.affiliation: Human Metabolome Technologies, Inc., Tsuruoka, Yamagata, Japan.
authors.forename: Kosaku
authors.initials: K
authors.lastname: Shinoda
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
mesh_terms: D000465:Algorithms; D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins