title: Paraffin tissue microarrays constructed with a cutting board and cutting board arrayer.
abstract: Paraffin tissue microarrays (PTMAs) are blocks of paraffin containing up to 1300 paraffin tissue core biopsies (PTCBs). Normally, these PTCBs are punched from routine paraffin tissue blocks, which contain tissues of differing thicknesses. Therefore, the PTCBs are of different lengths. In consequence, the sections of the deeper portions of the PTMA do not contain all of the desired PTCBs. To overcome this drawback, cutting boards were constructed from panels of plastic with a thickness of 4 mm. Holes were drilled into the plastic and filled completely with at least one PTCB per hole. After being trimmed to a uniform length of 4 mm, these PTCBs were pushed from the cutting board into corresponding holes in a recipient block by means of a plate with steel pins. Up to 1000 sections per PTMA were cut without any significant loss of PTCBs, thereby increasing the efficacy of the PTMA technique.
authors.affiliation: Institute of Pathology, University Hospital, Eberhard Karls University, Tuebingen, Germany. ulrich.vogel@med.uni-tuebingen.de
authors.forename: Ulrich Felix
authors.initials: UF
authors.lastname: Vogel
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D056656:Lab-On-A-Chip Devices; D008099:Liver / Q000473:pathology*; D016612:Paraffin Embedding; D010969:Plastics; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D010969:Plastics