Ramipril enhances the endogenous hydrogen sulfide tissue concentration in mouse heart and brain.
BACKGROUND
Hydrogen sulfide (H2S) participates in regulation of different physiological and pathological processes of cardiovascular system and nervous system. The effect of ramipril - tissue specific angiotensin-converting enzyme inhibitor (ACEI) - exceeds renin-angiotensin aldosterone system (RAAS) blockade. The influence of ACEI on the metabolism of H2S remains obscure. The aim of the study is to assess the impact of ramipril administration on the tissue concentration of H2S in mouse heart and brain.


METHODS
Thirty mice (CBA) of both sexes were given intraperitoneal injections of ramipril solution - 0.125 mg (5 mg/kg - group D1) and 0.25 mg (10 mg/kg - group D2) for 5 consecutive days at the same time of the day (10:30 am). Control group received physiological saline in portions of the same volume - 0.2 ml. The measurements of the concentration of H2S were performed using spectrophotometric modified method of Siegel.


RESULTS
There has been a significance rise in the tissue concentration of H2S [microg/g] in the heart in group D1 (4.91 +/- 0.07 vs 5.54 +/- 0.12; p = 0.005) and group D2 (4.91 +/- 0.07 vs 5.94 +/- 0.08; p < 0.001) and in the brain in group D1 (0.61 +/- 0.03 vs 0.69 +/- 0.02; p = 0.02) and non-significant increase in the brain D2 group (0.61 +/- 0.03 vs 0.65 +/- 0.03; p = 0.16).


CONCLUSIONS
Ramipril enhances the concentration of endogenous H2S in mouse heart and brain.
Wiliński
Jerzy
J
I Klinika Kardiologii i Nadciśnienia Tetniczego, Collegium Medicum Uniwersytetu Jagiellońskiego, Kraków, Poland. putamen@interia.pl
Somogyi
Eugeniusz
E
Góralska
Marta
M
Wiliński
Bogdan
B
Czarnecka
Danuta
D
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism*; D017257:Ramipril / Q000008:administration & dosage* / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D017257:Ramipril; D006862:Hydrogen Sulfide