Whole genome expression analysis reveals differential effects of TiO2 nanotubes on vascular cells.
The response of primary human endothelial (ECs) and vascular smooth muscle cells (VSMCs) to TiO2 nanotube arrays is studied through gene expression analysis. Microarrays revealed that nanotubes enhanced EC proliferation and motility, decreased VSMC proliferation, and decreased expression of molecules involved in inflammation and coagulation in both cell types. Networks generated from significantly affected genes suggest that cells may be sensing nanotopographical cues via pathways previously implicated in sensing shear stress.
Peng
Lily
L
Department of Bioengineering and Therapeutic Sciences, Department of Physiology, University of California-San Francisco, San Francisco, CA 94158-2330, USA.
Barczak
Andrea J
AJ
Barbeau
Rebecca A
RA
Xiao
Yuanyuan
Y
LaTempa
Thomas J
TJ
Grimes
Craig A
CA
Desai
Tejal A
TA
D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D016678:Genome; D006801:Humans; D007249:Inflammation; D053768:Metal Nanoparticles / Q000737:chemistry*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D043942:Nanotubes / Q000737:chemistry*; D010641:Phenotype; D013314:Stress, Mechanical; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D014025:Titanium