title: Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA.
abstract: Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular proliferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C-terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-beta receptor promoter (TbetaR-II) DNA. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 42.66, b = 52, c = 99.78 A, and diffracted to a resolution of 2.2 A.
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-7696, USA.
authors.forename: Vinod B
authors.initials: VB
authors.lastname: Agarkar
authors.forename: Nigar D
authors.initials: ND
authors.lastname: Babayeva
authors.forename: Angie
authors.initials: A
authors.lastname: Rizzino
authors.forename: Tahir H
authors.initials: TH
authors.lastname: Tahirov
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C494436:Elf3 protein, mouse; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II