Optical monitoring of oxygen tension in cortical microvessels with confocal microscopy.
Evaluating cerebral oxygenation is of critical importance for the understanding of brain function and several neuropathologies. Although several techniques exist for measuring cerebral oxygenation in vivo, the most widely accepted techniques offer limited spatial resolution. We have developed a confocal imaging system for minimally invasive measurement of oxygen tension (pO(2)) in cerebral microvessels with high spatial and temporal resolution. The system relies on the phosphorescence quenching method using exogenous porphyrin-based dendritic oxygen probes. Here we present high-resolution phosphorescence images of cortical microvasculature and temporal pO(2) profiles from multiple locations in response to varied fraction of inspired oxygen and functional activation.
Yaseen
Mohammad A
MA
Photon Migration Imaging Laboratory, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA 02129, USA
Srinivasan
Vivek J
VJ
Sakadzić
Sava
S
Wu
Weicheng
W
Ruvinskaya
Svetlana
S
Vinogradov
Sergei A
SA
Boas
David A
DA
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000166:cytology / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D010092:Oximetry / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D010100:Oxygen