title: Acute infection of Chinese macaques by a CCR5-tropic SHIV carrying a primary HIV-1 subtype B' envelope.
abstract: The increasing prevalence of HIV-1 subtype B' in China and Southeast Asia calls for efforts to develop a relevant animal model to study viral transmission and pathogenesis. Because there are significant differences between subtype B' HIV-1 and other chimeric simian/human immunodeficiency viru (SHIVs) in the env gene, a novel SHIV, designated SHIV(B'WHU), was generated by replacing counterparts of SHIV(SF33) with tat/rev/vpu/env genes derived from a primary, CCR5-tropic, subtype B' HIV-1 strain of a Chinese patient. SHIV(B'WHU) was able to replicate in rhesus peripheral blood mononuclear cells and used CCR5 as its major coreceptor. Moreover, after serial passages in Chinese macaques, the in vivo infectivity of SHIV(B'WHU) was enhanced, yet no significant sequence changes were found in viral envelopes, and the virus did not change its CCR5-tropism. CD4(+) T-cell loss, however, was found in the intraepithelial lymphocytes of small intestines of infected macaques. Our findings have implications in understanding the early pathogenesis of SHIV(B'WHU) in Chinese macaques.
authors.affiliation: AIDS Center and Modern Virology Research Center, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Zhuang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Zhijiao
authors.initials: Z
authors.lastname: Tang
authors.forename: Juanjie
authors.initials: J
authors.lastname: Tang
authors.forename: Po
authors.initials: P
authors.lastname: Tien
authors.forename: Linqi
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001210:Asia, Southeastern; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002681:China; D004195:Disease Models, Animal; D005818:Genetic Engineering; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007421:Intestine, Small / Q000276:immunology; D008251:Macaca; D019713:Receptors, CCR5 / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology*; D011995:Recombination, Genetic; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity*; D056189:Viral Tropism; D014774:Virulence
chemical_list: D019713:Receptors, CCR5; D011991:Receptors, Virus