Study of the action of Se and Cu on the growth metabolism of Escherichia coli by microcalorimetry.
The biological effect of Se and Cu²(+) on Escherichia coli (E. coli) growth was studied by using a 3114/3236 TAM Air Isothermal Calorimeter, ampoule method, at 37°C. From the thermogenesis curves, the thermokinetic equations were established under different conditions. The kinetics showed that a low concentration of Se (1-10 μg/mL) promoted the growth of E. coli, and a high concentration of Se (>10 μg/mL) inhibited the growth, but the Cu²(+) was always inhibiting the growth of E. coli. Moreover, there was an antagonistic or positive synergistic effect of Se and Cu²(+) on E. coli in the different culture medium when Se was 1-10 μg/ml and Cu²(+) was 1-20 μg/ml. There was a negative synergistic effect of Se and Cu²(+) on E. coli when Se was higher than 10 μg/ml and Cu²(+) was higher than 20 μg/ml. The antagonistic or synergistic effect between Se and Cu²(+) on E. coli was related to the formation of Cu-Se complexes under the different experimental conditions chosen.
Ding
Ling
L
Wuhan University of Science and Technology, Hubei, People's Republic of China. dingling@wust.edu.cn
Li
Xi
X
Liu
Peng
P
Li
Shiqian
S
Lv
Jiliang
J
D002151:Calorimetry; D003300:Copper / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004357:Drug Synergism; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D007700:Kinetics; D012643:Selenium / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*
D003300:Copper; D012643:Selenium