[Effect of 1,4-benzoquinone on growth of hematopoietic myeloid progenitor cells with IFN-gamma different genotypes].
This study was aimed to investigate the effect of 1,4-benzoquinone (1,4-BQ) on growth of myeloid progenitor cells with IFN-gamma different genotypes and to compare its differences. Polymerase chain reaction (PCR) was used to amplify the polymorphism gene segment of IFN-gamma +874 A/T in 36 cord blood (CB) specimens. The specimens were divided into three groups (AA, AT and TT group). MNCs were planted on complete methylcellulose medium containing different concentrations of 1,4-BQ. The colony-forming units (CFU) were assayed, the differences of colony growth in specimens with different genotypes (AA, AT and TT) under 1,4-BQ exposure were analyzed. The results showed that frequencies of AA, AT and TT genotypes were 5.56%, 88.89% and 5.56% in the 36 CB samples respectively. Comparing colony numbers of IFN-gamma +874 AA, AT and TT genotype indicated that there was significant difference (p(AA) = 0.033, p(AT) = 0.009, p(TT) = 0.001, < 0.05). Significant cytotoxicity was observed after exposure to concentrations of 1,4-BQ > or = 5 micromol/L. Cytotoxic response of 1,4-BQ was dose-dependent. Under the same concentration of 1,4-BQ, there were no significant differences in capacity of cell colony growth between 3 groups (AA, AT and TT). Colony numbers of specimen No 3 in AT group and specimen No 2 in TT group were less than those of other specimens significantly. It is concluded that the hematopoietic myeloid progenitor cells cultured in the presence of 1,4-BQ show a dose-dependent cytotoxic response, but there are no significant differences in colony growth of IFN-gamma different genotypes (AA, AT and TT) under the same concentration of 1,4-BQ.
Wang
Cai-Xia
CX
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Meng
Wen-Tong
WT
Chang
Hong
H
D016227:Benzoquinones / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D013234:Stem Cells
D016227:Benzoquinones; C004532:quinone; D007371:Interferon-gamma