title: Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA into the infected cell.
abstract: Loss of up to four amino acids from the C terminus of the 1318 residue bacteriophage T7 gp16 allows plaque formation at normal efficiencies. Loss of five residues results in non-infective virions, and loss of twelve prevents assembly of stable particles. However, replacing the C-terminal seven with nineteen non-native residues allows assembly of non-infective virions. The latter adsorb and eject internal core proteins into the cell envelope but no phage DNA enters the cytoplasm. Extragenic suppressors of the defective gene 16 lie in gene 15; the mutant gp15 proteins not only re-establish infectivity, they fully restore the kinetics of genome internalization to those exhibited by wild-type phage. After ejection from the infecting particle, gp15 and gp16 thus function together in ratcheting the leading end of the T7 genome into the cytoplasm of the infected cell.
authors.affiliation: Section of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Chang
authors.forename: Priscilla
authors.initials: P
authors.lastname: Kemp
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Molineux
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002462:Cell Membrane / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000821:virology; D016153:Genes, Suppressor / Q000502:physiology; D005814:Genes, Viral / Q000235:genetics / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000502:physiology*; D014771:Virion / Q000235:genetics / Q000502:physiology
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C065037:Gp16 protein, Bacteriophage T4; D014764:Viral Proteins