title: Crystallization and preliminary X-ray crystallographic analysis of DNA gyrase GyrB subunit from Xanthomonas oryzae pv. oryzae.
abstract: DNA gyrase is a type II topoisomerase that is essential for chromosome segregation and cell division owing to its ability to modify the topological forms of bacterial DNA. In this study, the N-terminal fragment of the GyrB subunit of DNA gyrase from Xanthomonas oryzae pv. oryzae was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.10 A resolution using a synchrotron-radiation source. The crystal belonged to space group I4(1), with unit-cell parameters a = b = 110.27, c = 70.75 A. The asymmetric unit contained one molecule, with a V(M) of 2.57 A(3) Da(-1) and a solvent content of 50.2%.
authors.affiliation: Department of Chemistry, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ha Yun
authors.initials: HY
authors.lastname: Jung
authors.forename: Ki Jeung
authors.initials: KJ
authors.lastname: Lee
authors.forename: Kyung Ha
authors.initials: KH
authors.lastname: Kim
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hyoung
authors.forename: Mi Ra
authors.initials: MR
authors.lastname: Han
authors.forename: Hyun Kyoung
authors.initials: HK
authors.lastname: Kim
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
authors.forename: Yeh-Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Heo
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D027081:DNA Gyrase / Q000737:chemistry*; D014974:Xanthomonas
chemical_list: D027081:DNA Gyrase