Evaluation of Iranian microbiology laboratories for identification of etiologic agents of bacterial meningitidis. Survey results of an external quality assessment scheme (EQAS) programme.
OBJECTIVE
To determine the ability of Iranian microbiology laboratories for identification and susceptibility testing of Streptococcus pneumoniae and Haemophilus influenzae as causative agents of bacterial meningitides.


METHODS
Two strains of bacteria including Haemophilus influenzae and Streptococcus pneumoniae as a common causative agents of meningitides were chosen and coded as strain number 1 and number 2. The strains were distributed among 679 microbiology laboratories. All laboratories were requested for identification of each unknown microorganism and susceptibility testing of S. pneumoniae against five commonly used antibiotics.


RESULTS
Of 679 microbiology laboratories 310 (46%) laboratories participated in the survey and among these, 258 laboratories completely identified S. pneumoniae. About 85% laboratories produced correct susceptibility testing against oxacillin, erythromycin, tetracycline, and vancomycin. Of 310 received responses only 50 laboratories identified H. influenza correctly. The majority of the laboratories did not have the capacity to identification H. influenza.


CONCLUSIONS
Microbiology laboratories in our country are qualified for identification and susceptibility testing of S. pneumoniae. However, majority of laboratories are not qualified for identification of H. influenzae.
Marandi
Farinaz Rashed
FR
Department of Microbiology, Iranian Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Rahbar
Mohammad
M
Sabourian
Roghieh
R
Saremi
Mahnaz
M
D019411:Clinical Laboratory Techniques / Q000592:standards*; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D007492:Iran; D007753:Laboratories / Q000592:standards*; D016920:Meningitis, Bacterial / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000592:standards; D011785:Quality Assurance, Health Care; D013296:Streptococcus pneumoniae / Q000302:isolation & purification*