[Computer-aided-designing and eukaryotic expression of recombinant HPV 16 vcaccine].
OBJECTIVE
To develop a recombinant protein vaccine with epitopes on L1 protein for prevention of HPV16 infection.


METHODS
One B cell epitope and one T cell epitope from HPV16 L1 protein were chosen to construct genes encoding different recombinant proteins. Then all the 3D structures of the proteins were predicted by Geno3D in internet. An optimal protein named RH was chosen in which three B epitopes' location simulates that in the real HPV16 L1. Gene X (consisting of B+T+T) and gene Y (consisting of B+T) were constructed by multiple PCR. RH gene (consisting of X+X+X+X+Y+Y+Y) was constructed, sub-cloned into pFastbac1, and expressed in Sf9.


RESULTS
RH protein with epitopes on HPV16 L1 was designed and predicted by Geno3D. RH gene was constructed and sub cloned into pFastbac1. Finally, it was successfully expressed in baculovirus expression system.


CONCLUSIONS
A HPV16 recombinant vaccine is successfully constructed and expressed.
Ren
Ji-ling
JL
Department of Immunology, Norman Bethune Medical College, Jilin University, Changchun 130021, China. jiren05@163.com
Wang
Hua
H
Yang
Ming
M
Wang
Li
L
Wu
Xiu-li
XL
Wang
Li-ying
LY
Yu
Yong-li
YL
D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D017076:Computer-Aided Design; D015870:Gene Expression; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D053918:Papillomavirus Vaccines / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering; D017434:Protein Structure, Tertiary
D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; C063317:L1 protein, Human papillomavirus type 16