Proteomic analysis reveals the dynamic association of proteins with translated mRNAs in Trypanosoma cruzi.
Gene regulation is mainly post-transcriptional in trypanosomatids. The stability of mRNA and access to polysomes are thought to be tightly regulated, allowing Trypanosoma cruzi to adapt to the different environmental conditions during its life cycle. Post-transcriptional regulation requires the association between mRNAs and certain proteins to form mRNP complexes. We investigated the dynamic association between proteins and mRNAs, using poly(T) beads to isolate and characterize proteins and protein complexes bound to poly-A+ mRNAs. The protein content of these fractions was analyzed by mass spectrometry (LC-MS/MS). We identified 542 protein component of the mRNP complexes associated with mRNAs. Twenty-four of the proteins obtained were present in all fractions, whereas some other proteins were exclusive to a particular fraction: epimastigote polysomal (0.37%) and post-polysomal (2.95%) fractions; stress polysomal (13.8%) and post-polysomal (40.78%) fractions. Several proteins known to be involved in mRNA metabolism were identified, and this was considered important as it made it possible to confirm the reliability of our mRNP isolation approach. This procedure allowed us to have a first insight into the composition and dynamics of mRNPs in T. cruzi.
Alves
Lysangela R
LR
Instituto Carlos Chagas, Laboratório de Regulação da Expressão Gênica, Curitiba, Paraná, Brazil.
Avila
Andréa R
AR
Correa
Alejandro
A
Holetz
Fabíola B
FB
Mansur
Fernanda C B
FC
Manque
Patrício A
PA
de Menezes
Juliana P B
JP
Buck
Gregory A
GA
Krieger
Marco A
MA
Goldenberg
Samuel
S
D000818:Animals; D011132:Polyribosomes / Q000737:chemistry; D011485:Protein Binding; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D020543:Proteome; D015800:Protozoan Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein