title: Chitosan-based hydrogels do not induce angiogenesis.
abstract: The aim of this study was to assess the angiogenic potential of chitosan-glycerol phosphate (GP)-hydroxyethyl cellulose (HEC) binder for injectable bone tissue engineering applications. The angiogenic response of chitosan-GP-HEC combined with and without human bone marrow-derived mesenchymal stem cells (hMSCs) was examined using the chick chorioallantoic membrane (CAM) assay. Chitosan-GP-HEC gel did not show any angiogenic potential, whereas the presence of hMSCs gave rise to an enhanced angiogenic response when placed on the CAM for 3 days. Quantitatively, significantly more blood vessel formation was observed for the stem cell-containing group as compared to all other groups (p < 0.05), except for the b-FGF-positive control. The results indicate that the chitosan-GP-HEC binder does not contribute to enhanced angiogenesis and that the presence of hMSCs enhances angiogenesis.
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Raheleh
authors.initials: R
authors.lastname: Ahmadi
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Burns
authors.forename: Joost D
authors.initials: JD
authors.lastname: de Bruijn
mesh_terms: D000328:Adult; D000818:Animals; D001681:Biological Assay; D001808:Blood Vessels / Q000187:drug effects / Q000254:growth & development; D002130:Calcium Phosphates / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D055252:Cell-Derived Microparticles / Q000378:metabolism; D002642:Chick Embryo; D048271:Chitosan / Q000494:pharmacology*; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects*
chemical_list: D002130:Calcium Phosphates; D020100:Hydrogels; D048271:Chitosan; C020243:calcium phosphate