title: Immunological reactivity of blood from healthy humans to the rAls3p-N vaccine protein.
abstract: We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.
authors.affiliation: Divisions of Infectious Diseases, Los Angeles Biomedical Research Institute, Harbor-University of California at Los Angeles (UCLA) Medical Center, USA.
authors.forename: Beverlie
authors.initials: B
authors.lastname: Baquir
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Ashraf S
authors.initials: AS
authors.lastname: Ibrahim
authors.forename: Yue
authors.initials: Y
authors.lastname: Fu
authors.forename: Valentina
authors.initials: V
authors.lastname: Avanesian
authors.forename: Ang
authors.initials: A
authors.lastname: Tu
authors.forename: John
authors.initials: J
authors.lastname: Edwards
authors.forename: Brad
authors.initials: B
authors.lastname: Spellberg
mesh_terms: D000908:Antibodies, Fungal / Q000097:blood*; D002175:Candida / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000276:immunology*; D005657:Fungal Vaccines / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood
chemical_list: D000908:Antibodies, Fungal; D016207:Cytokines; D005656:Fungal Proteins; D005657:Fungal Vaccines; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E