title: [Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction].
abstract: A kit of specific oligonucleotide primers and hybridization probes has been proposed to detect orthopoxviruses (OPV) and to discriminate human pathogenic viruses, such as variola virus and monkey virus by real-time polymerase chain reaction (PCR). For real-time PCR, the following pairs of fluorophore and a fluorescence quencher were used: TAMRA-BHQ2 for genus-specific probes and FAM-BHQ1 for species-specific ones (variola virus, monkeypox virus, ectomelia virus). The specificity of this assay was tested on 38 strains of 6 OPV species and it was 100%.
authors.forename: E V
authors.initials: EV
authors.lastname: Kostina
authors.forename: E V
authors.initials: EV
authors.lastname: Gavrilova
authors.forename: V A
authors.initials: VA
authors.lastname: Riabinin
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D045908:Mpox, Monkeypox / Q000175:diagnosis* / Q000235:genetics; D008993:Monkeypox virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012899:Smallpox / Q000175:diagnosis* / Q000235:genetics; D012901:Variola virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral