Effects of beta-ionone on mammary carcinogenesis and antioxidant status in rats treated with DMBA.
Recent chemopreventive studies from our group showed that dietary beta -ionone inhibited 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinogenesis by the inhibition of cell proliferation and apoptosis initiation. In this study, we examined the chemopreventive effects of varied doses of dietary beta -ionone on the development and growth of DMBA-induced rat mammary tumors as well as plasma antioxidant status. beta -ionone treatment groups were given 9, 18, and 36 mmol/kg in the AIN76A diet starting 2 wk prior to DMBA administration and continuing for the 24 wk. Results showed that tumor incidence was dose dependently reduced by 35.4, 68.3, and 87.8%, respectively, compared to the positive control. Tumor sizes were dose dependently smaller, and tumor weight was less in each group, each rat, and each tumor compared to the positive control (P < 0.05). A significant decrease in lipid peroxidation was observed in the tumor-induced rats treated with dietary beta -ionone, whereas the plasma activities of antioxidant enzymes such as glutathione peroxidase, glutathione reductase, superoxide dismutase, and the nonenzymatic antioxidant glutathione were increased in the beta -ionone treated rats when compared to control. The levels of catalase and lactate dehydrogenase were remarkably decreased in the beta -ionone treated groups compared to the positive control group. These results suggest that dietary beta -ionone has biologically relevant antioxidant activity and plays a chemopreventive role against DMBA induced mammary gland tumors.
Liu
Jia-Ren
JR
Public Health College, Harbin Medical University, NanGang District, Harbin, P. R. China.
Dong
Hong-Wei
HW
Sun
Xiang-Rong
XR
Wang
Qi
Q
Sun
Wen-Guang
WG
Parry
John W
JW
Liu
Qian
Q
Han
Xiao-Hui
XH
Sun
Chang-Hao
CH
Chen
Bing-Qing
BQ
Yang
Bao-Feng
BF
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000032:analysis*; D002273:Carcinogens; D002374:Catalase / Q000097:blood; D004032:Diet; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation; D008325:Mammary Neoplasms, Experimental / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D045792:Norisoprenoids / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D002273:Carcinogens; D045792:Norisoprenoids; D015127:9,10-Dimethyl-1,2-benzanthracene; C008157:beta-ionone; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione