Activation of human and chicken toll-like receptors by Campylobacter spp.
Campylobacter infection in humans is accompanied by severe inflammation of the intestinal mucosa, in contrast to colonization of chicken. The basis for the differential host response is unknown. Toll-like receptors (TLRs) sense and respond to microbes in the body and participate in the induction of an inflammatory response. Thus far, the interaction of Campylobacter with chicken TLRs has not been studied. Here, we investigated the potential of four Campylobacter strains to activate human TLR1/2/6, TLR4, TLR5, and TLR9 and chicken TLR2t2/16, TLR4, TLR5, and TLR21. Live bacteria showed no or very limited potential to activate TLR2, TLR4, and TLR5 of both the human and chicken species, with minor but significant differences between Campylobacter strains. In contrast, lysed bacteria induced strong NF-kappaB activation through human TLR1/2/6 and TLR4 and chicken TLR2t2/16 and TLR4 but not via TLR5 of either species. Interestingly, C. jejuni induced TLR4-mediated beta interferon in human but not chicken cells. Furthermore, isolated chromosomal Campylobacter DNA was unable to activate human TLR9 in our system, whereas chicken TLR21 was activated by DNA from all of the campylobacters tested. Our data are the first comparison of TLR-induced immune responses in humans and chickens. The results suggest that differences in bacterial cell wall integrity and in TLR responses to Campylobacter LOS and/or DNA may contribute to the distinct clinical manifestation between the species.
de Zoete
Marcel R
MR
Department of Infectious Diseases and Immunology, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands.
Keestra
A Marijke
AM
Roszczenko
Paula
P
van Putten
Jos P M
JP
D000818:Animals; D001419:Bacteria; D002167:Campylobacter / Q000276:immunology*; D002169:Campylobacter Infections / Q000276:immunology* / Q000662:veterinary*; D016123:Campylobacter jejuni; D002353:Carrier State / Q000276:immunology* / Q000662:veterinary*; D002645:Chickens / Q000276:immunology*; D004269:DNA, Bacterial / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology*
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D051193:Toll-Like Receptors; C023023:lipid-linked oligosaccharides