title: Catestatin, an endogenous chromogranin A-derived peptide, inhibits in vitro growth of Plasmodium falciparum.
abstract: Catestatin, an endogenous peptide derived from bovine chromogranin A, and its active domain cateslytin display powerful antimicrobial activities. We have tested the activities of catestatin and other related peptides on the growth of Plasmodium falciparum in vitro. Catestatin inhibits growth of the chloroquine-sensitive strain of P. falciparum 3D7, exhibiting 88% inhibition at 20 microM. A similar partial inhibition of parasite growth was observed for the chloroquine-resistant strain, 7G8 (64%,) and the multidrug-resistant strain, W2 (62%). In the presence of parasite-specific lactate dehydrogenase, a specific protein-protein interaction between catestatin and plasmepsin II precursor was demonstrated. In addition, catestatin partially inhibited the parasite-specific proteases plasmepsin in vitro. A specific interaction between catestatin and plasmepsins II and IV from P. falciparum and plasmepsin IV from the three remaining species of Plasmodium known to infect man was observed, suggesting a catestatin-induced reduction in availability of nutrients for protein synthesis in the parasite.
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale, Université de Strasbourg, France.
authors.forename: Aziza
authors.initials: A
authors.lastname: Akaddar
authors.forename: Cécile
authors.initials: C
authors.lastname: Doderer-Lang
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Marzahn
authors.forename: François
authors.initials: F
authors.lastname: Delalande
authors.forename: Marc
authors.initials: M
authors.lastname: Mousli
authors.forename: Karen
authors.initials: K
authors.lastname: Helle
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Dominique
authors.initials: D
authors.lastname: Aunis
authors.forename: Ben M
authors.initials: BM
authors.lastname: Dunn
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Metz-Boutigue
authors.forename: Ermanno
authors.initials: E
authors.lastname: Candolfi
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors; D002417:Cattle; D053379:Chromogranin A / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development*; D013329:Structure-Activity Relationship
chemical_list: D053379:Chromogranin A; D010446:Peptide Fragments; C109084:chromogranin A (344-364); D007770:L-Lactate Dehydrogenase; D016282:Aspartic Acid Endopeptidases; C089643:plasmepsin