[Comparative research of different Bupleurum chinense composition to influence of hepatotoxicity of rats and oxidative damage mechanism].
OBJECTIVE
To compare the influence of different Bupleurun chinense composition to the degree of hepatotoxicity damage to rats and oxidative damage mechanism.


METHODS
To successively lavage alcohol extracted and water extracted B. chinense composition to rats for 30 days, the general conditions were observed and the related index of liver function, the content of total-SH in serum, the content of MDA, the activity of SOD and the content and activity of GSH and GSH-Px in serum and liver tissue were detected.


RESULTS
Alcohol and water extracted B. chinense composition all could induce the increases of the activity of ALT and AST in serum, liver weight and the ratio of liver to body, and the content of MDA and induce the decreses of the content of total -SH in serum, the content of GSH, and the activity of SOD and GSH-Px in serum and liver tissue. The above-mentioned changes gradually aggravated with dose increasing, and there was significant difference compared with control group with distilled water.


CONCLUSIONS
The different B. chinense composition all can induce hepatotoxicity damage, and the channel of hepatic damage is related with the peroxidative damage mechanism. The degree of hepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition.
Lv
Lili
L
Shandong Research Academy of Traditional Chinese Medicine, Jinan 250014, China.
Huang
Wei
W
Yu
Xiao
X
Ren
Haiyong
H
Sun
Rong
R
D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D010936:Plant Extracts; C000820:semidehydroascorbic acid; D013482:Superoxide Dismutase; D003683:Dehydroascorbic Acid