Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths.
Mate-finding communication in many moths is mediated by sex pheromones produced by females. Since the differentiation of sex pheromones is often associated with speciation, it is intriguing to elucidate how the changes in sex pheromones are tracked by the pheromone recognition system of the males. Moths of the genus Ostrinia, which show distinct differentiation in female sex pheromones, are good models to study this. The present study was initiated with the aim of identifying ORs from Ostrinia scapulalis that respond to its own pheromone components, (E)-11- and (Z)-11-tetradecenyl acetates. We isolated six OR gene candidates (OscaOR3-8) from O. scapulalis. The same set of genes homologous to OscaOR3-8 were conserved in all (eight) Ostrinia species examined in addition to the previously reported OscaOR1 (tuned to (E)-11-tetradecenol) and the Or83b homologue OscaOR2. OscaOR3 not only responded to (E)-11- and (Z)-11-tetradecenyl acetates, but also to the pheromone components of the congeners, (Z)-9-, (E)-12-, and (Z)-12-tetradecenyl acetates. OscaOR4 responded with a relatively high specificity to (E)-11-tetradecenyl acetate. While OscaOR5 responded only marginally to a few pheromone components, OscaOR6-8 did not respond to any of the compounds tested. A few conserved ORs, including a unique one with very broad responsiveness, appear to be involved in the sex pheromone reception in O. scapulalis. The findings of the present study are discussed with reference to knowledge on electrophysiological response profiles of olfactory receptor neurons in Ostrinia moths.
Miura
Nami
N
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
Nakagawa
Tatsuro
T
Touhara
Kazushige
K
Ishikawa
Yukio
Y
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012724:Sex Attractants / Q000378:metabolism*; D013045:Species Specificity
D019476:Insect Proteins; D018035:Receptors, Odorant; D012724:Sex Attractants