Immune recovery effects of immunopotentiator from Pantoea agglomerans 1 (IP-PA1) on low antibody productions in response to Salmonella enteritidis vaccine and sheep red blood cells in dexamethasone-treated stressed chicken models.
Considering the usefulness of the immunopotentiator from Pantoea agglomerans 1 (IP-PA1), which is a purified lipopolysaccharide (LPS) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. Three-week-old chickens daily administered 10 microg/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relative thymic, bursal, and splenic weight losses; and decrease in the number of peripheral blood lymphocytes, as compared with the control chickens on day 35; the IP-PA1-pretreated, dexamethasone-treated chickens showed reduced weight losses. Five- to eight-week-old chickens administered 5 mg/kg of dexamethasone showed excessive apoptosis of thymic and bursal lymphocytes 24 hr after a single dexamethasone treatment; apoptosis was inhibited in the IP-PA1-pretreated, dexamethasone-treated chickens. Chickens daily administered 10 microg/kg of dexamethasone for 35 days and injected with commercial Salmonella Enteritidis (SE) vaccine or sheep red blood cells (SRBC) on days 7 and 21 showed about 8- or 2-fold lower antibody production in response to SE or SRBC, respectively, as compared with the control chickens on day 35; the antibody production in response to SE or SRBC was increased in the IP-PA1-pretreated, dexamethasone-treated chickens. These results indicate that IP-PA1 exerts inhibitory effects on dexamethasone-induced immunosuppression and that it may be useful in controlling immunosuppression in poultry husbandry.
Hebishima
Takehisa
T
Laboratory of Veterinary Physiology Division of Animal Life Science, Institute of Symbiotic Science and Technology, Graduate School, Tokyo University of Agriculture and Technology.
Matsumoto
Yasunobu
Y
Soma
Gen-Ichiro
G
Kohchi
Chie
C
Watanabe
Gen
G
Taya
Kazuyoshi
K
Hayashi
Yoshihiro
Y
Hirota
Yoshikazu
Y
D000818:Animals; D000917:Antibody Formation; D017209:Apoptosis; D002645:Chickens; D003907:Dexamethasone / Q000627:therapeutic use; D004756:Enterobacteriaceae Infections / Q000276:immunology* / Q000662:veterinary*; D017707:Erythrocyte Transfusion / Q000662:veterinary*; D007165:Immunosuppression Therapy / Q000379:methods / Q000662:veterinary; D008214:Lymphocytes / Q000187:drug effects; D020636:Pantoea; D011201:Poultry Diseases / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000276:immunology*; D012756:Sheep; D013950:Thymus Gland / Q000187:drug effects; D015431:Weight Loss
D003907:Dexamethasone