Identification of a 0.4 Kb deletion region in 10q26 associated with endometrial carcinoma.
We have identified an allelic deletion common region in the q26 region of chromosome 10 in endometrial carcinomas, which has been reported previously as a potential target of genetic alterations related to this neoplasia. An allelotyping analysis of 19 pairs of tumoral and non-tumoral samples was accomplished using seven microsatellite polymorphic markers mapping in the 10q26 chromosomal region. Loss of heterozygosity for one or more loci was detected in 29% of the endometrial carcinoma samples. The observed pattern of loss enabled the identification of a 3.5 Mb common deleted region located between the D10S587 and D10S186 markers. An additional result from an endometrial sample with evidence of a RER phenotype may suggest a more centromeric region of loss within the above-mentioned interval. This 401.84 Kb interval flanked by the D10S587 and D10S216 markers may be a plausible location for a putative suppressor gene involved in early stage endometrial carcinogenesis.
Alves
Margarida
M
Faculdade de Farmácia Universidade de Lisboa, Lisboa, Portugal.
Carreira
Isabel
I
Liberato
Paulo
P
Ramos
Sância
S
Mafra
Manuela
M
Inverno
Alexandra S
AS
Maia
Ana T
AT
Martins
Ana Paula
AP
Brito
Miguel
M
Monteiro
Carolino
C
D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D004261:DNA Replication / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D016555:Matched-Pair Analysis; D053842:Microsatellite Instability