title: Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits.
abstract: The GTPase dynamin, a key player in endocytic membrane fission, interacts with numerous proteins that regulate actin dynamics and generate/sense membrane curvature. To determine the functional relationship between these proteins and dynamin, we have analyzed endocytic intermediates that accumulate in cells that lack dynamin (derived from dynamin 1 and 2 double conditional knockout mice). In these cells, actin-nucleating proteins, actin, and BAR domain proteins accumulate at the base of arrested endocytic clathrin-coated pits, where they support the growth of dynamic long tubular necks. These results, which we show reflect the sequence of events in wild-type cells, demonstrate a concerted action of these proteins prior to, and independent of, dynamin and emphasize similarities between clathrin-mediated endocytosis in yeast and higher eukaryotes. Our data also demonstrate that the relationship between dynamin and actin is intimately connected to dynamin's endocytic role and that dynamin terminates a powerful actin- and BAR protein-dependent tubulating activity.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Ferguson
authors.forename: Shawn
authors.initials: S
authors.lastname: Ferguson
authors.forename: Andrea
authors.initials: A
authors.lastname: Raimondi
authors.forename: Summer
authors.initials: S
authors.lastname: Paradise
authors.forename: Hongying
authors.initials: H
authors.lastname: Shen
authors.forename: Kumi
authors.initials: K
authors.lastname: Mesaki
authors.forename: Agnes
authors.initials: A
authors.lastname: Ferguson
authors.forename: Olivier
authors.initials: O
authors.lastname: Destaing
authors.forename: Genevieve
authors.initials: G
authors.lastname: Ko
authors.forename: Junko
authors.initials: J
authors.lastname: Takasaki
authors.forename: Ottavio
authors.initials: O
authors.lastname: Cremona
authors.forename: Eileen
authors.initials: E
authors.lastname: O' Toole
authors.forename: Pietro
authors.initials: P
authors.lastname: De Camilli
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D034284:Dynamin I / Q000235:genetics / Q000378:metabolism; D034285:Dynamin II / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis; D055786:Gene Knockout Techniques; D051379:Mice; D018345:Mice, Knockout
chemical_list: D000199:Actins; D002966:Clathrin; D034284:Dynamin I; D034285:Dynamin II