title: Changes of CD4+CD25high regulatory T cells and FOXP3 expression in unexplained recurrent spontaneous abortion patients.
abstract: OBJECTIVE
  To investigate the proportions of CD4+CD25high T cells and forkhead box p3 (FOXP3) expression in peripheral blood and decidua in patients with unexplained recurrent spontaneous abortion (URSA).
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  125 URSA patients, 35 normal early pregnant women, and 28 normal nonpregnant women.
  
  
  METHODS
  Measurements of CD4+CD25high T cells and FOXP3 expression in peripheral blood and decidua.
  
  
  METHODS
  The proportions of CD4+CD25high T cells and FOXP3 expression.
  
  
  RESULTS
  In peripheral blood, statistically significantly higher proportions of CD4+CD25high T cells and FOXP3 expression were observed in normal early pregnant women compared with normal nonpregnant women and URSA patients; a statistically significantly lower proportion of CD4+CD25high T cells was observed in nonpregnant URSA patients compared with URSA patients who had early miscarriages and normal nonpregnant women. In the decidua, statistically significantly lower proportions of CD4+CD25high T cells and FOXP3 expression were found in URSA patients with early miscarriages compared with normal early pregnant women.
  
  
  CONCLUSIONS
  The CD4+CD25high T cells may play an important role in maintaining a normal pregnancy. The reduction in CD4+CD25high T cells may involve in the pathogenesis of URSA, and is correlated with lower FOXP3 expression.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Mei
authors.forename: Jianping
authors.initials: J
authors.lastname: Tan
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000026:Abortion, Habitual / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D003656:Decidua / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit