title: [Anthelmintic activity of aqueous and ethanolic extracts of Morinda citrifolia fruit on Ascaridia galli].
abstract: The anthelmintic activity of aqueous and ethanolic extracts of Morinda citrifolia fruit (noni) was evaluated in chicken naturally infected by Ascaridia galli. The anthelmintic activity in vitro was determined in adult parasites. The aqueous and ethanolic extracts were used in the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL(-1) and 4.17; 8.34; 16.68; 33.36 and 66.72 mg.mL(-1), respectively. The anthelmintic activity in vivo was determined by the administration of 10 mL.kg(-1) of the aqueous (50.1 mg.mL(-1)) and ethanolic (24.6 mg.mL(-1)) extracts during three consecutive days. Later the chickens were euthanized and necropsy was performed in order to count the remaining helminths. The data were analyzed by the Student-Newman-Keuls test. In the concentrations of 13.48 and 26.96 mg.mL(-1), the aqueous extract demonstrated mortality of 46.67 and 50%, respectively, there was a significative difference from the negative control (P < 0.05). The ethanolic extract presented statistical difference from the negative control (diluent) (P < 0.05) for the concentrations of 33.36 and 66.72 mg.mL(-1), expressed by a mortality rate of 66.67 and 76.67%, respectively. In the in vivo test, the aqueous extract of noni fruit showed 27.08% of elimination, deferring statistically from the control group. There was no statistical difference between the ethanolic extract treatments and the control (P > 0.05). It follows that the anthelmintic activity of noni fruit test showed satisfactory results in vitro, there is a need for studies in higher concentrations in the in vivo test.
authors.affiliation: Departamento de Morfofisiologia Veterinária, Universidade Federal do Piauí, Teresina, Brazil.
authors.forename: Danilo R Barros
authors.initials: DR
authors.lastname: Brito
authors.forename: Rozeverter Moreno
authors.initials: RM
authors.lastname: Fernandes
authors.forename: Maria Zenaide de Lima C M
authors.initials: MZ
authors.lastname: Fernandes
authors.forename: Marcos Daniel de S
authors.initials: MD
authors.lastname: Ferreira
authors.forename: Fernanda R L
authors.initials: FR
authors.lastname: Rolim
authors.forename: Manoel L
authors.initials: ML
authors.lastname: da Silva Filho
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001197:Ascaridia / Q000187:drug effects*; D000431:Ethanol; D005638:Fruit; D032066:Morinda; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D000431:Ethanol