The genome-wide expression profile of electroacupuncture in DNP-KLH immunized mice.
Previously, we demonstrated that electoracupuncture (EA) suppressed allergic reactions in DNP-KLH immunized mice. In this study, the mechanisms by which EA induces immunomodulation in the immunized mice were evaluated by genome-wide microarray analysis. The anti-allergic effects of EA in DNP-KLH immunized mice were confirmed by analyzing antigen specific IgE using ELISA. Microarray analysis, followed by real time RT-PCR validation, revealed that Th1 and Th17 cytokine-, opioid peptide-, and anti-apoptosis-related genes were up-regulated upon treatment with EA. In addition, significant decreases in Th2 cytokine-, MAPK signaling pathway-, and apoptosis-related genes were observed following EA treatment.
Sohn
Sung-Hwa
SH
Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, 130-701, Republic of Korea.
Kim
Sun Kwang
SK
Ko
Eunjung
E
Lee
Youngseop
Y
Chung
Hwan-Suck
HS
Lee
Hyojung
H
Kim
Hyunseong
H
Hwang
Deok-Sang
DS
Nam
Sangsoo
S
Bae
Hyunsu
H
D019297:2,4-Dinitrophenol / Q000276:immunology; D000276:Adjuvants, Immunologic; D000818:Animals; D016207:Cytokines / Q000276:immunology; D015671:Electroacupuncture; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000628:therapy*; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D015203:Reproducibility of Results; D014475:Uncoupling Agents
D000276:Adjuvants, Immunologic; D016207:Cytokines; D014475:Uncoupling Agents; D007073:Immunoglobulin E; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D019297:2,4-Dinitrophenol