title: Effect of changes in number of doses and anatomic location for administration of an Escherichia coli bacterin on serum IgG1 and IgG2 concentrations in dairy cows.
abstract: OBJECTIVE
  To determine effects of injection site on antibody response to J5 Escherichia coli bacterin.
  
  
  METHODS
  28 adult Holstein cows.
  
  
  METHODS
  Cows were randomly assigned as control cattle (n = 4 cows), not administered J5 E coli bacterin; 3X (8), administered 3 doses of bacterin SC in the left side of the neck; 5XN (8), administered 5 doses of bacterin SC in the left side of the neck; or 5XSR (8), administered 5 doses of bacterin SC sequentially in the left side of the neck, right side of the neck, right side of the thorax, left side of the thorax, and left side of the neck. Blood samples were collected from the cows to determine anti-J5 E coli IgG1 and IgG2 concentrations.
  
  
  RESULTS
  Vaccinated cows had higher mean serum anti-J5 E coli IgG1 concentrations than did control cows. The 5XN and 5XSR cows had higher mean serum anti-J5 E coli IgG1 concentrations than did 3X cows. Additionally, 5XSR cows had higher mean serum anti-J5 E coli IgG1 concentrations than did 5XN cows. Vaccinated cows had higher mean serum anti-J5 E coli IgG2 concentrations than did control cows. The 5XN and 5XSR cows had higher mean serum anti-J5 E coli IgG2 concentrations than did 3X cows. The 5XSR cows had higher mean serum anti-J5 E coli IgG2 concentrations than did all other groups at 84 days after the fifth vaccination.
  
  
  CONCLUSIONS
  Sequential doses of core-antigen bacterins administered at different anatomic locations may improve antibody response in dairy cattle.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. Erskine@cvm.msu.edu
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Erskine
authors.forename: Anne R
authors.initials: AR
authors.lastname: Brockett
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Beeching
authors.forename: Robert W
authors.initials: RW
authors.lastname: Hull
authors.forename: Paul C
authors.initials: PC
authors.lastname: Bartlett
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D002417:Cattle; D004334:Drug Administration Schedule; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G