Temperature dependence of the sodium pump is altered in the cerebral cortex of CCK2 receptor-deficient mice.
Previously we have shown that the temperature dependence of the sodium pump (Na(+),K(+)-ATPase) is altered under different neuropathological conditions. In this study we compared temperature dependence of the Na(+),K(+)-ATPase in the fronto-parietal cortex of CCK(2) receptor-deficient (homo- and heterozygous) and normal (wild-type) mice. The Arrhenius plot for Na(+),K(+)-ATPase from wild-type brain is non-linear with a breakpoint at 20.3 +/- 0.4 degrees C. In case of the brain cell membrane of CCK(2) receptor-deficient mice (homo- and heterozygous) the breakpoint on Arrhenius plot was detected at 26.0 +/- 1.1 degrees C and 25.4 +/- 0.4 degrees C, respectively. The shift of the breakpoint on the Arrhenius plot established in CCK(2) receptor-deficiency as well as in case of some other pathological conditions confirms that such kind of alteration in the Na(+),K(+)-ATPase temperature dependence is likely related to the homeostatic adjustment of altered function of the sodium pump.
Salum
T
T
Department of Biochemistry, Faculty of Medicine, Tartu University, Ravila 19, Tartu, Estonia. tiit.salum@kliinikum.ee
Kõks
S
S
Kairane
C
C
Mahlapuu
R
R
Zilmer
M
M
Vasar
E
E
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D006579:Heterozygote; D006720:Homozygote; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043887:Receptor, Cholecystokinin B / Q000172:deficiency*; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000502:physiology*; D013696:Temperature
D043887:Receptor, Cholecystokinin B; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase