title: [Characterization of Bacillus thuringiensis spoIII D gene mutation].
abstract: OBJECTIVE
  Construction and characterization of a spoIII D gene deletion mutant of Bacillus thuringiensis.
  
  
  METHODS
  Scanning electron microscopy and spore formation analysis were used to detect the ability of sporulation and formation of crystal protein in both the mutant and the wild strain. SDS-PAGE analysis was used to detect the expression of crystal protein.
  
  
  RESULTS
  Scanning electron microscopy and spore formation analysis showed that spores were hardly produced and the crystal existed in the spoIII D deletion strain. SDS-PAGE results showed that the expression of cry gene in the mutant was decreased in Luria-Bertani medium, but not affected obviously in Schaeffer's sporulation medium (SSM).
  
  
  CONCLUSIONS
  This indicated that the spoIII D gene was one of the essential genes for the sporulation of Bacillus thuringiensis, and influenced the expression of crystal protein.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. peregrine2018@gmail.com
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Changlong
authors.initials: C
authors.lastname: Shu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Dafang
authors.initials: D
authors.lastname: Huang
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C071977:SpoIIID protein, Bacillus; D014157:Transcription Factors