[Genetic damage of mammalian cells induced by nickel-refining fumes].
OBJECTIVE
To study the effects of two kinds of nickel-refining fumes on DNA damage of NIH/3T3 cell and the difference.


METHODS
NIH/3T3 cells were treated by two kinds of nickel fumes collected from smelting furnace and refining workshop of a nickel-smeltery, and PBS taken the place of nickel-smelting fumes was used as negative control. Several hours later, the cytotoxicity of on NIH/3T3 cells was detected with MTT colorimetric assay, and the DNA damage was also measured by comet assay (single cell gel electrophoresis).


RESULTS
With the extension of exposure time and increasing of concentration, the living rate of NIH/3T3 cells was decreased; the tail rate, tail extent moment and tail DNA percent of NIH/3T3 cell induced by these two refining fumes were increased. After cells were treated with 100.00 microg/ml of nickel-smelting fume for 48 h, the living rate of NIH/3T3 cells was 24.5% and 26.5% respectively. The tail length of NIH/3T3 cell induced by these two refining fumes was not significant difference. Tail DNA percent of NIH/3T3 cell induced by smelting furnace fume was higher than negative control group (P < 0.05). The tail rate, and tail DNA percent (except 12.5 microg/ml and 50.0 microg/ml treated 2 h group) of NIH/3T3 cell induced by refining workshop fume was higher than negative control group (P < 0.05).


CONCLUSIONS
Nickel-smelting fume could depress the survival rate of NIH/3T3 cells, and induce different degree DNA damage of NIH/3T3 cell.
Jin
Yan-Tao
YT
School of Public Health, Harbin Medical University, Harbin 150081, China.
Wu
Yong-Hui
YH
Yu
Cui-Ping
CP
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D008669:Metallurgy; D051379:Mice; D041681:NIH 3T3 Cells; D009532:Nickel / Q000633:toxicity*
D009532:Nickel