Recombinant canine coronaviruses in dogs, Europe.
Coronaviruses of potential recombinant origin with porcine transmissible gastroenteritis virus (TGEV), referred to as a new subtype (IIb) of canine coronavirus (CCoV), were recently identified in dogs in Europe. To assess the distribution of the TGEV-like CCoV subtype, during 2001-2008 we tested fecal samples from dogs with gastroenteritis. Of 1,172 samples, 493 (42.06%) were positive for CCoV. CCoV-II was found in 218 samples, and CCoV-I and CCoV-II genotypes were found in 182. Approximately 20% of the samples with CCoV-II had the TGEV-like subtype; detection rates varied according to geographic origin. The highest and lowest rates of prevalence for CCoV-II infection were found in samples from Hungary and Greece (96.87% and 3.45%, respectively). Sequence and phylogenetic analyses showed that the CCoV-IIb strains were related to prototype TGEV-like strains in the 5' and the 3' ends of the spike protein gene.
Decaro
Nicola
N
Department of Veterinary Public Health, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
Mari
Viviana
V
Elia
Gabriella
G
Addie
Diane D
DD
Camero
Michele
M
Lucente
Maria Stella
MS
Martella
Vito
V
Buonavoglia
Canio
C
D000818:Animals; D001483:Base Sequence; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000821:virology; D017939:Coronavirus, Canine / Q000235:genetics*; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology; D004285:Dogs / Q000821:virology; D005060:Europe / Q000453:epidemiology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D005838:Genotype; D006115:Greece / Q000453:epidemiology; D006814:Hungary / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D005760:Transmissible gastroenteritis virus / Q000235:genetics