Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells.
Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Ialpha and Ibeta and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Ialpha and Ibeta led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-gamma, tumor necrosis factor-alpha, granulocyte-colony stimulating factor or transforming growth factor-beta1. The elevated IL-6 expression seen in REG Ialpha transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology.
Usami
Shuetsu
S
Department of Biochemistry, Akita University Graduate School of Medicine, Akita, Japan.
Motoyama
Satoru
S
Koyota
Souichi
S
Wang
Jingshu
J
Hayashi-Shibuya
Kaori
K
Maruyama
Kiyotomi
K
Takahashi
Naoko
N
Saito
Hajime
H
Minamiya
Yoshihiro
Y
Takasawa
Shin
S
Ogawa
Jun-Ichi
J
Sugiyama
Toshihiro
T
D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D051847:Lithostathine / Q000235:genetics / Q000378:metabolism*; D018307:Neoplasms, Squamous Cell / Q000378:metabolism*; D014162:Transfection
D015850:Interleukin-6; D051847:Lithostathine; C497617:REG1A protein, human; C497618:REG1B protein, human