title: Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains.
abstract: Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of S. cerevisiae.
authors.affiliation: LCPME, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Nancy-University, CNRS, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, France. joel.coulon@pharma.uhp-nancy.fr
authors.forename: Joël
authors.initials: J
authors.lastname: Coulon
authors.forename: Ilan
authors.initials: I
authors.lastname: Thouvenin
authors.forename: Fadi
authors.initials: F
authors.lastname: Aldeek
authors.forename: Lavinia
authors.initials: L
authors.lastname: Balan
authors.forename: Raphaël
authors.initials: R
authors.lastname: Schneider
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D005453:Fluorescence; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006031:Glycosylation; D007716:Kluyveromyces / Q000737:chemistry / Q000302:isolation & purification*; D037102:Lectins / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009172:Mycology / Q000379:methods*; D045663:Quantum Dots; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000302:isolation & purification*; D013691:Tellurium / Q000737:chemistry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D019187:Cadmium Compounds; D037102:Lectins; D014867:Water; D005947:Glucose; D013691:Tellurium; D008358:Mannose; C028337:cadmium telluride; D005690:Galactose