Flavonoids from the buds of Rosa damascena inhibit the activity of 3-hydroxy-3-methylglutaryl-coenzyme a reductase and angiotensin I-converting enzyme.
Rosa damascena has been manufactured as various food products, including tea, in Korea. A new flavonoid glycoside, kaempferol-3-O-beta-D-glucopyranosyl(1-->4)-beta-D-xylopyranoside, named roxyloside A was isolated from the buds of this plant, along with four known compounds, isoquercitrin, afzelin, cyanidin-3-O-beta-glucoside, and quercetin gentiobioside. The chemical structures of these compounds were determined by spectroscopic analyses, including FAB-MS, UV, IR, (1)H and (13)C NMR, DEPT, and 2D NMR (COSY, HSQC, and HMBC). All the isolated compounds except cyanidin-3-O-beta-glucoside exhibited high levels of inhibitory activity against 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase with IC(50) values ranging from 47.1 to 80.6 microM. Cyanidin-3-O-beta-glucoside significantly suppressed angiotensin I-converting enzyme (ACE) activity, with an IC(50) value of 138.8 microM, while the other four compounds were ineffective. These results indicate that R. damascena and its flavonoids may be effective to improve the cardiovascular system.
Kwon
Eun-Kyung
EK
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, South Korea.
Lee
Dae-Young
DY
Lee
Hyungjae
H
Kim
Dae-Ok
DO
Baek
Nam-In
NI
Kim
Young-Eon
YE
Kim
Hae-Yeong
HY
D000214:Acyl Coenzyme A / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D007700:Kinetics; D008168:Lung / Q000737:chemistry / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*
D000214:Acyl Coenzyme A; D000806:Angiotensin-Converting Enzyme Inhibitors; D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D007703:Peptidyl-Dipeptidase A