title: GAD67-GFP knock-in mice have normal sleep-wake patterns and sleep homeostasis.
abstract: Gamma-aminobutyric acid (GABA) ergic neurons are important for controlling sleep and wakefulness but are difficult to identify, limiting their study. Knock-in mice with GABAergic neurons labeled by expression of green fluorescent protein (GFP) under control of the glutamate decarboxylase 67 (GAD67) promoter are now extensively used in neuroscience. However, it is unknown whether these mice have a normal sleep phenotype. Compared with wild-type control mice, GAD67-GFP knock-in mice had the same amount of non-rapid eye movement (NREM) sleep and rapid-eye movement (REM) sleep, a similar diurnal distribution of sleep, no NREM or REM sleep differences in electroencephalogram power, and normal sleep rebound following 6-h sleep deprivation. Our results suggest GAD67-GFP knock-in mice are an excellent tool for study of GABAergic neurons involved in sleep-wake regulation.
authors.affiliation: Laboratory of Neuroscience, VA Boston Healthcare System and Department of Psychiatry, Harvard Medical School, Brockton, Massachusetts, USA.
authors.forename: Lichao
authors.initials: L
authors.lastname: Chen
authors.forename: James T
authors.initials: JT
authors.lastname: McKenna
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Leonard
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Robert W
authors.initials: RW
authors.lastname: McCarley
authors.forename: Ritchie E
authors.initials: RE
authors.lastname: Brown
mesh_terms: D000818:Animals; D004569:Electroencephalography; D005260:Female; D055879:Gene Knock-In Techniques; D005968:Glutamate Decarboxylase / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006706:Homeostasis / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D012890:Sleep / Q000235:genetics*; D014851:Wakefulness / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1