Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis.
Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.
Xiao
Yan
Y
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy, West China Hospital, West China School of Medicine, Sichuan University, 610041 Chengdu, People's Republic of China.
Lv
Xiaoyan
X
Cao
Ge
G
Bian
Guohui
G
Duan
Jingjing
J
Ai
Jianzhong
J
Sun
Huan
H
Li
Qingwei
Q
Yang
Qiutan
Q
Chen
Tielin
T
Zhao
Danhua
D
Tan
Ruizhi
R
Liu
Yuhang
Y
Wang
Yidong
Y
Zhang
Zheng
Z
Yang
Yang
Y
Wei
Yuquan
Y
Zhou
Qin
Q
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D049109:Cell Proliferation; D004285:Dogs; D004594:Electrophysiology; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050396:TRPP Cation Channels / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D050396:TRPP Cation Channels; D002118:Calcium