title: KIR genotyping by multiplex PCR-SSP.
abstract: Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.
authors.affiliation: Cancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, MD, USA.
authors.forename: Smita
authors.initials: S
authors.lastname: Kulkarni
authors.forename: Maureen P
authors.initials: MP
authors.lastname: Martin
authors.forename: Mary
authors.initials: M
authors.lastname: Carrington
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D017931:DNA Primers; D054340:Receptors, KIR