title: Serum albumin induces osmotic swelling of rat retinal glial cells.
abstract: Edema in the ischemic neural tissue develops by increased vascular permeability associated with extravasation of albumin, and by glial swelling. Here, we show that bovine serum albumin acutely administered to slices of the rat retina causes swelling of glial somata under hypoosmotic conditions. The effect of albumin was dose-dependent, with half-maximal and maximal effects at 10 nM and 1 microM, respectively, and was mediated by activation of transforming growth factor-beta receptor type II, oxidative stress, and the production of arachidonic acid and prostaglandins. Albumin-induced glial swelling was prevented by glutamate and purinergic receptor agonists. The data suggest that serum albumin may induce glial swelling in the presence of osmotic gradients.
authors.affiliation: Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Silvana
authors.initials: S
authors.lastname: Löffler
authors.forename: Antje
authors.initials: A
authors.lastname: Wurm
authors.forename: Franziska
authors.initials: F
authors.lastname: Kutzera
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.forename: Katja
authors.initials: K
authors.lastname: Krügel
authors.forename: Regina
authors.initials: R
authors.lastname: Linnertz
authors.forename: Peter
authors.initials: P
authors.lastname: Wiedemann
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Andreas
authors.initials: A
authors.lastname: Bringmann
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002417:Cattle; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D066298:In Vitro Techniques; D042541:Intracellular Space / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009995:Osmosis / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011453:Prostaglandins / Q000378:metabolism; D058913:Purinergic Agonists; D051381:Rats; D020318:Rats, Long-Evans; D017470:Receptors, Glutamate / Q000378:metabolism; D011983:Receptors, Purinergic / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D012160:Retina / Q000187:drug effects / Q000503:physiopathology*; D012710:Serum Albumin, Bovine / Q000378:metabolism*
chemical_list: D018690:Excitatory Amino Acid Agonists; D018377:Neurotransmitter Agents; D011453:Prostaglandins; D058913:Purinergic Agonists; D017470:Receptors, Glutamate; D011983:Receptors, Purinergic; D018125:Receptors, Transforming Growth Factor beta; D012710:Serum Albumin, Bovine; D016718:Arachidonic Acid