title: Lagerstroemia speciosa extract inhibit TNF-induced activation of nuclear factor-kappaB in rat cardiomyocyte H9c2 cells.
abstract: OBJECTIVE
  Lagerstroemia speciosa has been used as a folk medicine among people with diabetes in the Philippines. It is known to exhibit antidiabetic, antiobesity, and glucose transport activities through mechanisms not well defined. Diabetes leads to cardiomyocyte hypertrophy in association with an upregulation of vasoactive factors and activation of nuclear factor (NF)-kappaB and activating protein-1. We therefore investigated the effect of Lagerstroemia speciosa on the activation of NF-kappaB as a key mediator of cardiomyocyte hypertrophy, in rat cardiomyocyte H9c2 cells.
  
  
  METHODS
  Water extract of Lagerstroemia speciosa (Lythraceae family) was prepared. H9c2 cells were used for treatment of Lagerstroemia speciosa extract with/without tumor necrosis factor (TNF). To examine NF-kappaB's activation, we performed an electrophoretic mobility shift assay (EMSA).
  
  
  RESULTS
  The activation of NF-kappaB by TNF was completely blocked by a Lagerstroemia speciosa extract in a dose- and time-dependent manner in H9c2 cells.
  
  
  CONCLUSIONS
  Overall, our results indicate that Lagerstroemia speciosa can inhibit DNA-binding of NF-kappaB. This may explain its possible inhibition of diabetes-induced cardiomyocyte hypertrophy.
authors.affiliation: International Research and Educational Institute for Integrated Medical Sciences, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. ichikawa@imcir.twmu.ac.jp
authors.forename: Haruyo
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Hisato
authors.initials: H
authors.lastname: Yagi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Jong-Chol
authors.initials: JC
authors.lastname: Cyong
authors.forename: Tomoh
authors.initials: T
authors.lastname: Masaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D016325:HIV Long Terminal Repeat; D006321:Heart / Q000187:drug effects*; D031563:Lagerstroemia / Q000737:chemistry*; D009206:Myocardium / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha