title: Microstructure and ultrastructure of high-amylose rice resistant starch granules modified by antisense RNA inhibition of starch branching enzyme.
abstract: A high-amylose transgenic rice line (TRS) modified by antisense RNA inhibition of starch branching enzymes revealed a resistant starch-rich quality. Compound starch granules in whole grains of the regular rice cultivar Teqing (TQ) were readily split during fracturing, whereas the starch granules in TRS were structurally intact and showed large voluminous, non-angular rounded bodies and elongated, filamentous structures tolerant of fracturing. In isolated preparation, TQ starch granules broke up into separate polygonal granules, whereas TRS starch granules kept their intactness. TRS starch granules consisted of packed smaller subgranules, some of which located at the periphery of starch granules were fused to each other with adjacent ones forming a thick band or wall encircling the entire circumference of the granules. TQ starch granules had a high concentration of amylose in the concentric hilum, whereas TRS starch granules showed a relatively even distribution of amylose with intense amylose in both hilum and band.
authors.affiliation: Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
authors.forename: Cunxu
authors.initials: C
authors.lastname: Wei
authors.forename: Fengling
authors.initials: F
authors.lastname: Qin
authors.forename: Lijia
authors.initials: L
authors.lastname: Zhu
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Yifang
authors.initials: Y
authors.lastname: Chen
authors.forename: Youping
authors.initials: Y
authors.lastname: Wang
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.forename: Qiaoquan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000235:genetics* / Q000378:metabolism; D000688:Amylose / Q000032:analysis*; D012275:Oryza / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D016372:RNA, Antisense / Q000235:genetics*; D013213:Starch / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D010940:Plant Proteins; D016372:RNA, Antisense; D013213:Starch; D000688:Amylose; D015061:1,4-alpha-Glucan Branching Enzyme