Evaluation of resveratrol oxidation in vitro and the crucial role of bicarbonate ions.
Polyphenols can oxidize in culture medium and produce artifacts in cell culture studies. However, the extent and mechanism of the oxidation of resveratrol, a polyphenol abundant in red wine, is unclear. We investigated the oxidation of resveratrol in vitro and the effects of various components of the culture medium on the degradation of resveratrol and the production of H(2)O(2). We found that 96% of resveratrol at a concentration of 200 microM was degraded in Base Modified Eagle Medium after 24 h of incubation at 37 degrees C, producing about 90 microM of H(2)O(2). Including sodium bicarbonate in the medium markedly stimulated resveratrol degradation and H(2)O(2) production. In sum, we found that bicarbonate ions played a crucial role in the oxidative degradation of resveratrol in vitro, and that the degradation of resveratrol can be avoided by withdrawing sodium bicarbonate from the medium. A mechanism for the oxidation of resveratrol is proposed.
Yang
Nae-Cherng
NC
Department of Nutrition and Health Science, Chungchou Institute of Technology, Changhua, Taiwan. naeboy@dragon.ccut.edu.tw
Lee
Cheng-Hao
CH
Song
Tuzz-Ying
TY
D001639:Bicarbonates / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D006531:HEPES / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010710:Phosphates / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D000077185:Resveratrol; D017693:Sodium Bicarbonate / Q000378:metabolism; D013267:Stilbenes / Q000378:metabolism*; D014325:Tromethamine / Q000378:metabolism; D014867:Water / Q000378:metabolism
D001639:Bicarbonates; D000440:Butanols; D003470:Culture Media; D010710:Phosphates; D013267:Stilbenes; D014325:Tromethamine; D014867:Water; D019289:Pyruvic Acid; D017693:Sodium Bicarbonate; D002392:Catechin; C045651:epigallocatechin gallate; D002374:Catalase; D009584:Nitrogen; D000077185:Resveratrol; D006531:HEPES; C018279:sodium phosphate