Mechanisms of ATP-dependent nucleosome sliding.
Chromatin remodelers are multifunctional protein machines that use a conserved ATPase motor to slide nucleosomes along DNA. Nucleosome sliding has been proposed to occur through two mechanisms: twist diffusion and loop/bulge propagation. A central idea for both of these models is that a DNA distortion propagates over the surface of the nucleosome. Recent data from biochemical and single-molecule experiments have expanded our understanding of histone-DNA and remodeler-nucleosome interactions, and called into question some of the basic assumptions on which these models were originally based. Advantages and challenges of several nucleosome sliding models are discussed.
Bowman
Gregory D
GD
T C Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218-2685, USA. gdbowman@jhu.edu
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism*
D006657:Histones; D009707:Nucleosomes; D000255:Adenosine Triphosphate; D004247:DNA; D000251:Adenosine Triphosphatases