Array-based sensing of normal, cancerous, and metastatic cells using conjugated fluorescent polymers.
A family of conjugated fluorescent polymers was used to create an array for cell sensing. Fluorescent conjugated polymers with pendant charged residues provided multivalent interactions with cell membranes, allowing the detection of subtle differences between different cell types on the basis of cell surface features. Highly reproducible characteristic patterns were obtained from different cell types as well as from isogenic cell lines, enabling the identification of the cell type as well differentiating between normal, cancerous, and metastatic isogenic cell types with high accuracy.
Bajaj
Avinash
A
Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.
Miranda
Oscar R
OR
Phillips
Ronnie
R
Kim
Ik-Bum
IB
Jerry
D Joseph
DJ
Bunz
Uwe H F
UH
Rotello
Vincent M
VM
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D016002:Discriminant Analysis; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity
D011108:Polymers