title: Molecular modelling insights into DFNA15 mediated enhancement of POU4F3 stability.
abstract: The POU4F3 transcription factor is expressed in the cochlear and vestibular hair cells of the inner ear and its targeted deletion results in a loss of inner ear hair cells. The DFNA15 truncation mutation has been demonstrated to result in a loss of transcriptional activity, but an increase in the stability of the protein. Molecular modelling is utilised to propose a mechanism of stability enhancement, via an interaction between the truncated POU(HD) domain and the POU(S) domain of the transcription factor.
authors.affiliation: Department of Otolaryngology, University of Liege, CHU de Liege, 4000 Liege, Belgium. cfrenz@gmail.com
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Frenz
authors.forename: Philippe P
authors.initials: PP
authors.lastname: Lefebvre
mesh_terms: D019295:Computational Biology; D003198:Computer Simulation; D030562:Databases, Protein; D006207:Half-Life; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008958:Models, Molecular; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability; D017384:Sequence Deletion; D055672:Static Electricity; D040681:Structural Homology, Protein; D013816:Thermodynamics; D050821:Transcription Factor Brn-3C / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; C111223:POU4F3 protein, human; D050821:Transcription Factor Brn-3C