Purification, crystallization and initial X-ray diffraction study of human REV7 in complex with a REV3 fragment.
REV7 is involved in various cellular functions including DNA replication, signal transduction and cell-cycle regulation. In DNA replication, REV7 interacts with REV3 and forms DNA polymerase zeta, which plays a central role in error-prone DNA synthesis. REV3 is a catalytic subunit and its activity is stimulated by REV7. To clarify the structural basis of the interaction between REV7 and REV3, human REV7 was crystallized in complex with a REV3 fragment. Two crystal forms were obtained. Crystal forms I and II belonged to space groups P2(1), with unit-cell parameters a = 43.8, b = 50.0, c = 107.3 A, beta = 96.9 degrees , and P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 76.6, c = 118.4 A, respectively.
Hara
Kodai
K
Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
Shimizu
Toshiyuki
T
Unzai
Satoru
S
Akashi
Satoko
S
Sato
Mamoru
M
Hashimoto
Hiroshi
H
D019943:Amino Acid Substitution; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D064246:Mad2 Proteins; D046912:Multiprotein Complexes / Q000737:chemistry / Q000302:isolation & purification; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D004268:DNA-Binding Proteins; C120552:MAD2L2 protein, human; D064246:Mad2 Proteins; D046912:Multiprotein Complexes; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; D004259:DNA-Directed DNA Polymerase; C118285:REV3L protein, human