Gene expression profile of acupuncture treatment in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model.
OBJECTIVE
To find new biomarkers by stimulating acupuncture point GB34 (Yangneungcheon) which has neuroprotective effect on the mouse model of Parkinson's disease, analysis of cDNA microarray on mRNAs of the substantia nigra was performed.


METHODS
Male C57BL/6 mice were divided into two groups: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice (MPTP group, n=3); 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and acupuncture (GB34)-treated mice (MPTP + ACU group, n=3). The mice received an intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (30 mg/kg) once daily for 3 consecutive days. Manual acupuncture was performed 2 hours after every injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The total RNA in the substantia nigra of each mouse was isolated on 3 days after the last 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection. Agilent mouse whole genome 44K chip was used for microarray analysis and the hybridization image was analysed by GenePix Pro 6.0. Data normalization and analysis were performed using GeneSpring GX 7.3.1 program.


RESULTS
The acupuncture stimulation revealed 799 genes (424 up- and 375 down-regulated) of which expression levels were changed more than two-folds in the MPTP + ACU group, compared to the MPTP group. The genes selected were classified into several categories based on their functions using DAVID Bioinformatics Resources 2008 (http://david.abcc.ncifcrf.gov/) and KEGG PATHWAY Database (http://www.genome.jp/kegg/pathway.html).


CONCLUSIONS
Biomarkers in response to acupuncture stimulation to GB34 were identified in a mouse model for Parkinson's disease. These biomarkers might provide a promising clue for understanding the neuroprotective effect of acupuncture in Parkinson's disease.
Hong
Mee Sook
MS
Kohwang Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea.
Park
Hun-Kuk
HK
Yang
Jong-Soo
JS
Park
Hi-Joon
HJ
Kim
Seung-Tae
ST
Kim
Seung-Nam
SN
Park
Ji-Yeun
JY
Song
Jeong Yoon
JY
Park
Hyun-Kyung
HK
Jo
Dae Jean
DJ
Park
Sung Wook
SW
HwanYun
Dong
D
Ban
Ju Yeon
JY
Chung
Joo-Ho
JH
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015670:Acupuncture Therapy; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D030541:Databases, Genetic; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D020407:Internet; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D012984:Software; D013378:Substantia Nigra / Q000378:metabolism*; D013997:Time Factors
D015415:Biomarkers; D012333:RNA, Messenger; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine