title: [Inhibitory effect of dexamethasone on myeloid differentiation factor 88 and tumor necrosis factor-alpha expressions in mouse peritoneal macrophages].
abstract: OBJECTIVE
  To study the inhibitory effect of dexamethasone (DEX) on myeloid differentiation factor 88 (MyD88) and tumor necrosis factor-alpha (TNF-alpha) expression in mouse peritoneal macrophages in innate immune response to Penicillium marneffei (PM).
  
  
  METHODS
  Mouse peritoneal macrophages were cultured in the presence of heat-inactivated yeast-phase PM with or without DEX, and the protein and mRNA expressions of MyD88 in the macrophages were detected using Western blotting and real-time PCR, respectively. TNF-alpha in the cell culture supernatant was measured with enzyme-linked immunosorbent assay.
  
  
  RESULTS
  DEX suppressed TNF-alpha production by the macrophages co-cultured with PM. The expressions of MyD88 were up-regulated by PM stimulation, whose effect was inhibited by the application of DEX.
  
  
  CONCLUSIONS
  The inhibitory effect of DEX on PM-induced proinflammatory responses of the macrophage is directly associated with the inhibition of MyD88 expression.
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. zhaowenjie1972@sohu.com
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Xi
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Jun-min
authors.initials: JM
authors.lastname: Zhang
authors.forename: Xi-qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Chang-ming
authors.initials: CM
authors.lastname: Lu
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053594:Myeloid Differentiation Factor 88 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010407:Penicillium / Q000254:growth & development; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D053594:Myeloid Differentiation Factor 88; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone