[Synthesis and identification of sulfonamides artificial antigen].
OBJECTIVE
To synthesise N-sulfanil-4-aminobutyric acid mother nucleus derivative of sulfonamides, and its artificial antigens in order to explore the methods of preparation were discussed.


METHODS
N-sulfanil-4-aminobutyric acid was synthesized and confirmed by 1HNMR, 13CNMR, IR, ESI-MS. N-sulfanil-4-aminobutyric acid artificial antigen was synthesized by the methods of active ester coupling, and was identified by UV scan. Single factor test and orthogonal test design methods L9(3(3)) were employed to study the influence of the factors such as the initial molar ratio of reagents, activate hapten time and the coupling time to the coupling rate, as to determine the optimal reaction conditions for synthesis. The obtained results showed that the initial molar ratio of hapten to BSA 50:1, activated hapten time 6 hours, coupling time 4 hours. The artificial antigen was prepared by the optimal reaction conditions. The artificial antigen was injected into Balb/c mice and the blood samples were obtained from tails of Balb/c mice after the 5th injection and detected by iELISA method.


RESULTS
The structure of N-sulfanil-4-aminobutyric acid was correct. The artificial antigen was prepared.


CONCLUSIONS
Antigens was acquired successfully.
Cui
Yanhua
Y
School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. yhcui@hit.edu.cn
Zhang
Hailing
H
Ma
Ying
Y
Cui
Jie
J
D000613:Aminobutyrates / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology; D013425:Sulfanilic Acids / Q000737:chemistry* / Q000276:immunology; D013449:Sulfonamides / Q000737:chemistry* / Q000276:immunology
D000613:Aminobutyrates; D000906:Antibodies; D000941:Antigens; D006241:Haptens; C554240:N-sulfanil-4-aminobutyric acid; D013425:Sulfanilic Acids; D013449:Sulfonamides; D012710:Serum Albumin, Bovine