General functionalization route for cell adhesion on non-wetting surfaces.
We present a versatile route for promoting cell adhesion and viability on various non-wetting surfaces, inspired by mussel adhesion mechanism. The oxidative polymerization of dopamine, a small designer molecule of the DOPA-K motif found in mussels, results in the formation of a poly(dopamine) ad-layer on any material surface. We found that the poly(dopamine) coating can promote cell adhesion on any type of material surfaces including the well-known anti-adhesive substrate, poly(tetrafluoroethylene). According to our results, mammalian cells well adhered and underwent general cell adhesion processes (i.e., attachment to substrate, spreading, and cytoskeleton development) on poly(dopamine)-modified surfaces, while they barely adhered and spread on unmodified non-wetting surfaces. The mussel-inspired surface functionalization strategy is extremely useful because it does not require the time-consuming synthesis of complex linkers and the process is solvent-free and non-toxic. Therefore, it can be a powerful route for converting a variety of bioinert substrates into bioactive ones.
Ku
Sook Hee
SH
Department of Materials Science and Engineering, KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
Ryu
Jungki
J
Hong
Seon Ki
SK
Lee
Haeshin
H
Park
Chan Beum
CB
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape; D002470:Cell Survival; D004298:Dopamine / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D016716:PC12 Cells; D056951:Photoelectron Spectroscopy; D051381:Rats; D017155:Wettability / Q000187:drug effects
D004298:Dopamine