title: Inactivation and mechanisms of chlorine dioxide on Nosema bombycis.
abstract: Biological tests demonstrated that the inactivation of Nosema bombycis (N. bombycis) spores by chlorine dioxide (ClO(2)) occurs very fast and is highly sensitive. The lowest effective inactivation dosage and time was 15mg/mL for 30min. The inactivation of spores was additionally verified by using double color fluorescence stain and spore germination testing. A series of biological changes, including a large number of substrates that were leaked out from the spores included proteins, DNA, polysaccharide, K(+), and Ca(2+), occurred a short time after N. bombycis spores were treated with ClO(2). In addition, the lipid of spores was disrupted and ATPase activity was inhibited, which resulted in the destruction of the inner structure of the spores.
authors.affiliation: Department of Sericulture Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhengyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Fupin
authors.initials: F
authors.lastname: Liao
authors.forename: Jianrong
authors.initials: J
authors.lastname: Lin
authors.forename: Wenchu
authors.initials: W
authors.lastname: Li
authors.forename: Yangsheng
authors.initials: Y
authors.lastname: Zhong
authors.forename: Peichan
authors.initials: P
authors.lastname: Tan
authors.forename: Ziran
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D012831:Bombyx / Q000382:microbiology; D017606:Chlorine Compounds / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D016818:Nosema / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects*
chemical_list: D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide