title: Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release.
abstract: Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Ave Park, 4806-909 Taipas, Guimarães, Portugal. paulo.bessa@dep.uminho.pt
authors.forename: P C
authors.initials: PC
authors.lastname: Bessa
authors.forename: E R
authors.initials: ER
authors.lastname: Balmayor
authors.forename: H S
authors.initials: HS
authors.lastname: Azevedo
authors.forename: S
authors.initials: S
authors.lastname: Nürnberger
authors.forename: M
authors.initials: M
authors.lastname: Casal
authors.forename: M
authors.initials: M
authors.lastname: van Griensven
authors.forename: R L
authors.initials: RL
authors.lastname: Reis
authors.forename: H
authors.initials: H
authors.lastname: Redl
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000494:pharmacology; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D011994:Recombinant Proteins / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D055767:Immobilized Proteins; D011994:Recombinant Proteins; D014867:Water; D005349:Fibroins