pilF Polymorphism-based PCR to distinguish vibrio vulnificus strains potentially dangerous to public health.
Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.
Roig
Francisco J
FJ
Departamento de Microbiología, Facultad de Biología, Universidad de Valencia, Valencia 46100, Spain.
Sanjuán
Eva
E
Llorens
Amparo
A
Amaro
Carmen
C
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics*; D005399:Fishes; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000382:microbiology / Q000473:pathology; D041261:Vibrio vulnificus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors; D036781:Fimbriae Proteins