Whole-body three-dimensional optoacoustic tomography system for small animals.
We develop a system for three-dimensional whole-body optoacoustic tomography of small animals for applications in preclinical research. The tomographic images are obtained while the objects of study (phantoms or mice) are rotated within a sphere outlined by a concave arc-shaped array of 64 piezocomposite transducers. Two pulsed lasers operating in the near-IR spectral range (755 and 1064 nm) with an average pulsed energy of about 100 mJ, a repetition rate of 10 Hz, and a pulse duration of 15 to 75 ns are used as optical illumination sources. During the scan, the mouse is illuminated orthogonally to the array with two wide beams of light from a bifurcated fiber bundle. The system is capable of generating images of individual organs and blood vessels through the entire body of a mouse with spatial resolution of approximately 0.5 mm.
Brecht
Hans-Peter
HP
Fairway Medical Technologies, Inc., 9431 West Sam Houston Parkway South, Houston, Texas 77099, USA.
Su
Richard
R
Fronheiser
Matthew
M
Ermilov
Sergey A
SA
Conjusteau
Andre
A
Oraevsky
Alexander A
AA
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology; D006197:Hair; D006736:Horses; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D051379:Mice; D019047:Phantoms, Imaging; D012815:Signal Processing, Computer-Assisted; D041622:Tomography, Optical / Q000379:methods*; D051598:Whole Body Imaging / Q000379:methods*