title: Molecular characterisation of Vibrio cholerae O1 strains carrying an SXT/R391-like element from cholera outbreaks in Kenya: 1994-2007.
abstract: BACKGROUND
  Over the last decade, cholera outbreaks in parts of Kenya have become common. Although a number of recent studies describe the epidemiology of cholera in Kenya, there is paucity of information concerning the diversity and occurrence of mobile genetic elements in Vibrio cholerae strains implicated in these outbreaks. A total of 65 Vibrio cholerae O1 El Tor serotype Inaba isolated between 1994 and 2007 from various outbreaks in Kenya were investigated for mobile genetic elements including integrons, transposons, the integrating conjugative elements (ICEs), conjugative plasmids and for their genotypic relatedness.
  
  
  RESULTS
  All the strains were haemolytic on 5% sheep blood and positive for the Vibrio cholerae El Tor-specific haemolysin toxin gene (hylA) by PCR. They all contained strB, sulII, floR and the dfrA1 genes encoding resistance to streptomycin, sulfamethoxazole, chloramphenicol and trimethoprim respectively. These genes, together with an ICE belonging to the SXT/R391 family were transferable to the rifampicin-resistant E. coli C600 en bloc. All the strains were negative for integron class 1, 2 and 3 and for transposase gene of transposon Tn7 but were positive for integron class 4 and the trpM gene of transposon Tn21. No plasmids were isolated from any of the 65 strains. All the strains were also positive for all V. cholera El Tor pathogenic genes except the NAG- specific heat-stable toxin (st) gene. None of the strains were positive for virulence genes associated with the V. cholerae classical biotype. All the strains were positive for El Tor-specific CTXphi bacteriophage rstrR repressor gene (CTXETPhi) but negative for the Classical, Calcutta, and the Environmental repressor types. Pulse Field Gel Electrophoresis (PFGE) showed that regardless of the year of isolation, all the strains bearing the SXT element were clonally related.
  
  
  CONCLUSIONS
  This study demonstrates that the V. cholerae O1 strains carrying an SXT/R391-like element implicated in recent cholera outbreaks in Kenya has not changed significantly between 1994 and 2007 and are clonally related.
authors.affiliation: Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640, Nairobi, Kenya. jndemi@yahoo.com
authors.forename: John N
authors.initials: JN
authors.lastname: Kiiru
authors.forename: Suleiman M
authors.initials: SM
authors.lastname: Saidi
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Goddeeris
authors.forename: Njeri C
authors.initials: NC
authors.lastname: Wamae
authors.forename: Patrick
authors.initials: P
authors.lastname: Butaye
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Kariuki
mesh_terms: D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D044404:Genomic Islands; D005838:Genotype; D006801:Humans; D032023:Integrons; D007630:Kenya / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D041241:Vibrio cholerae O1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D037521:Virulence Factors