Applications of ultra-performance liquid chromatography to traditional Chinese medicines.
Ultra-performance liquid chromatography (UPLC), as a development of the chromatographic technique, was used in the quality control of Panax ginseng C.A. Mey. In the study, the UPLC methods were transferred from conventional high-performance liquid chromatography (HPLC) methods according to chromatographic equations. The results demonstrated that UPLC analysis methods could be transferred from HPLC without loss of efficiency. Compared with conventional HPLC, UPLC made a surprising 10-fold increase in speed and 20-fold decrease in solvent consumption. The study indicated UPLC as a suitable alternative to HPLC and can be reliably applied to quality control of traditional Chinese medicines.
Yang
Gui-Ling
GL
School of Life Science, Sun Yat-Sen University, Guangzhou, 510275, China.
Yang
Li-Wei
LW
Li
Yong-Xue
YX
Cao
Hui
H
Zhou
Wen-Liang
WL
Fang
Zhi-Jian
ZJ
Zhou
Hong-Bo
HB
Mo
Jie-Li
JL
Xiao
Shu-Xiong
SX
Lin
Hui-Rong
HR
D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides