Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis.
In the present paper, we have used 2-DE coupled to MS analysis to examine the molecular variability of the Ole e 1 allergen in three olive cultivars (cvs). Our results confirmed that the predicted polymorphism of Ole e 1 at cDNA level is extended to the expressed protein. The profiles of both the Ole e 1 peptides and the N-glycan variants significantly changed among cvs. We observed that Picual and Arbequina cvs presented the highest and lowest degree of Ole e 1 polymorphism, respectively. Some of these peptides and N-glycans were distributed in a cv-specific manner. The putative implications of this molecular polymorphism in the development of the allergy symptoms are discussed.
Castro
Antonio Jesús
AJ
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain. antoniojesus.castro@eez.csic.es
Bednarczyk
Audrey
A
Schaeffer-Reiss
Christine
C
Rodríguez-García
María Isabel
MI
Van Dorsselaer
Alain
A
Alché
Juan de Dios
JD
D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D052179:Antigens, Plant; D002853:Chromatography, Liquid; D003720:Densitometry; D015180:Electrophoresis, Gel, Two-Dimensional; D006020:Glycopeptides / Q000032:analysis*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D031658:Olea / Q000235:genetics*; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D010936:Plant Extracts; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis; D016622:Silver Staining
D000485:Allergens; D000906:Antibodies; D052179:Antigens, Plant; D006020:Glycopeptides; C081946:Ole e I protein, Olea europaea; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D020543:Proteome