title: Suppressive effects of a cyanine dye against herpes simplex virus (HSV)-1 infection.
abstract: In this study, we demonstrate that a cyanine dye, lumin, significantly suppressed cytopathic effect by herpes simplex virus (HSV)-1 toward human amnionic FL cell and also it reduced replication of HSV-1 in a dose-dependent manner. In addition, lumin additively augmented the antiviral effect of interferon (IFN)-alpha. Furthermore, fluorescence microscopic study showed that lumin (not IFN-alpha) itself remarkably induced alkalinization of intracellular organelle, suggesting the inhibition of virus invasion into the cells. These results suggest that lumin exerts an antiviral action against HSV-1 with the independent pathways of IFN-alpha and also it would become a therapeutically effective drug in clinical practice.
authors.affiliation: Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. fujisaki@hayashibara.co.jp
authors.forename: Chie
authors.initials: C
authors.lastname: Ushio
authors.forename: Harumi
authors.initials: H
authors.lastname: Ariyasu
authors.forename: Toshio
authors.initials: T
authors.lastname: Ariyasu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Arai
authors.forename: Tsunetaka
authors.initials: T
authors.lastname: Ohta
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology; D002232:Carbocyanines / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D011992:Endosomes / Q000737:chemistry / Q000187:drug effects; D006561:Herpes Simplex / Q000188:drug therapy*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016898:Interferon-alpha / Q000494:pharmacology / Q000627:therapeutic use; D011806:Quinolinium Compounds / Q000494:pharmacology / Q000627:therapeutic use; D014779:Virus Replication / Q000187:drug effects
chemical_list: D002232:Carbocyanines; D016898:Interferon-alpha; D011806:Quinolinium Compounds; C052194:lumin