KIR genotyping by multiplex PCR-SSP.
Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.
Kulkarni
Smita
S
Cancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, MD, USA.
Martin
Maureen P
MP
Carrington
Mary
M
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D054340:Receptors, KIR / Q000235:genetics*
D017931:DNA Primers; D054340:Receptors, KIR