Inflammatory and haematotoxic potential of indoor Stachybotrys chartarum (Ehrenb.) Hughes metabolites.
Mould Stachybotrys chartarum (Ehrenb.) Hughes is known to pose a health risk in indoor environments. Most of its strains can produce several intra- and extracellular trichothecene mycotoxins. Complex secondary metabolites of stachybotrys isolates from mouldy dwellings/public buildings in Slovakia were intratracheally instilled in Wistar male rats (4 microg in 0.2 mL of 0.2 % dimethylsulphoxide; diacetoxyscirpenol as the positive control). After three days, haematological parameters were measured in peripheral blood and inflammatory response biomarkers in bronchoalveolar lavage fluid (BALF), and the results were statistically analysed. Exometabolites proved to suppress red blood cell (RBC), decreasing the total RBC count, haemoglobin, and haematocrit. The exposed rats showed significantly higher total BALF cell count, indicating inflammation, lower alveolar macrophage counts, and increased granulocyte count related to the BALF cells. Due to haematotoxic and inflammation-inducing properties, metabolites of S. chartarum can cause damage to the airways and haematological disorders in occupants of mouldy buildings.
Piecková
Elena
E
Slovak Medical University, Bratislava, Slovakia. elena.pieckova@szu.sk
Hurbánková
Marta
M
Cerná
Silvia
S
Lisková
Aurélia
A
Kováciková
Zuzana
Z
Kolláriková
Zuzana
Z
Wimmerová
Sona
S
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004906:Erythrocyte Count; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis*; D007249:Inflammation; D008168:Lung / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000473:pathology; D008297:Male; D009183:Mycotoxins / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013191:Stachybotrys / Q000378:metabolism*
D006454:Hemoglobins; D009183:Mycotoxins