[Determination of trace free copper and zinc in serum samples by graphite furnace atomic absorption spectrometry after two-step precipitation of protein].
A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1 : 2. The mixture was subsequently denatured at 70 degrees C and centrifuged to precipitate proteins. The determination, limit of copper (3sigma) was 1.2 microg x L(-1) and the recovery was 92.3% - 104%, and the determination. Limit of zinc(3sigma) was 0.098 microg x L(-1) and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people.
Zhang
Yuan
Y
Central Laboratories, Shantou University Medical College, Shantou 515041, China. yzhang@stu.edu.cn
Lin
Zhe-Xuan
ZX
Li
Wei-Qiu
WQ
Zhang
Qiao-Xin
QX
Li
Hui
H
Luo
Hong-Jun
HJ
Luo
Wen-Hong
WH
D003300:Copper / Q000032:analysis*; D006108:Graphite; D006801:Humans; D009369:Neoplasms / Q000737:chemistry; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000032:analysis*
D006108:Graphite; D003300:Copper; D015032:Zinc