title: Effects of cycloartane saponins from hairy roots of Astragalus membranaceus Bge., on human tumor cell targets.
abstract: For the first time three different natural compounds, isolated from hairy roots of Astragalus membranaceus, cultivated in airlift bioreactor were tested for their cytotoxic potential and apoptosis induction in a panel of human tumor cell lines. Root cultures, cultivated in bioreactor gave 18.5 g l(-1) dry wt roots with the highest astragaloside production in vitro up to now - 1.64% (astragaloside I), 1.12% (astragaloside II) and 1.08% (astragaloside III). In this manner the production in airlift bioreactor can be used as means of reliable supply of cycloartane saponins to extend the research to human clinical studies.
authors.affiliation: Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria. ionkova@pharmfac.acad.bg
authors.forename: I
authors.initials: I
authors.lastname: Ionkova
authors.forename: G
authors.initials: G
authors.lastname: Momekov
authors.forename: P
authors.initials: P
authors.lastname: Proksch
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D027885:Astragalus propinquus / Q000737:chemistry*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A