title: Bing De Ling, a Chinese herbal formula, inhibits cancer cells growth via p53.
abstract: Bing De Ling is a Chinese herbal formula that has been used to treat cancer patients for more than a decade. However, the molecular mechanisms behind its anti-tumor efficacy are still elusive. Here, we show that Bing De Ling inhibits cell proliferation in ovarian cancer epithelial cell lines, OV2008 and C13. It induces G1/S arrest in a p53-dependent manner in that this effect is attenuated in OV2008 cells transfected with dominant-negative p53 plasmid. Moreover, we show that Bing De Ling up-regulates p53 transcriptional activities as well as its downstream target genes, such as p21Cip1, MDM2, and MDMX. In addition, Bing De Ling inhibits MDMX-p53 interaction which may result in stabilization and activation of p53. Collectively, our results suggest that the anti-tumor activity of Bing De Ling may be in part due to activation of p53.
authors.affiliation: Department of Pathology and Cell Biology, USF College of Medicine, Tampa, Florida 33612-4799, USA.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huiqin
authors.initials: H
authors.lastname: Dong
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.forename: Shengyan
authors.initials: S
authors.lastname: Xiang
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Mu
authors.initials: M
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Wenlong
authors.initials: W
authors.lastname: Bai
authors.forename: Santo V
authors.initials: SV
authors.lastname: Nicosia
authors.forename: Jiandong
authors.initials: J
authors.lastname: Chen
authors.forename: Ruan-Jin
authors.initials: RJ
authors.lastname: Zhao
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting; D008156:Luciferases; D009687:Nuclear Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; D004365:Drugs, Chinese Herbal; C482775:MDM4 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D008156:Luciferases; C022616:thiazolyl blue