An immunocompetent murine model of metastatic mammary cancer accessible to bioluminescence imaging.
BACKGROUND
Many areas of research, including gene and pharmacological therapeutics, would benefit from longitudinal in vivo monitoring methodologies. To investigate the feasibility of one such methodology, we developed a murine mammary cancer model amenable to sequential bioluminescent imaging of tumor growth and metastasis in living animals.


METHODS
Metastatic mouse mammary carcinoma BJMC3879 cells were transfected to stably express firefly luciferase and inoculated into immunocompetent female BALB/c mice.


RESULTS
Sequential analysis using bioluminescent imaging showed increasing photon counts correlated to expanding mammary tumor volumes; in addition, strong signals from axillary, mandibular, femoral, thoracic and abdominal regions in mice were histopathologically determined to be due to metastases, the majority of which occurred in lymph nodes and lungs.


CONCLUSIONS
The bioluminescent mouse mammary cancer model we established provides a method for quantifiable longitudinal in vivo imaging that can be used in gene and pharmacological therapy applications.
Shibata
Masa-Aki
MA
Department of Anatomy and Cell Biology, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan. shibatam@art.osaka-med.ac.jp
Shibata
Eiko
E
Morimoto
Junji
J
Eid
Nabil A S
NA
Tanaka
Yoshihisa
Y
Watanabe
Masahito
M
Otsuki
Yoshinori
Y
D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D049409:Luciferases, Firefly / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D014162:Transfection
D049409:Luciferases, Firefly