Selective detection of hydroxyl radical scavenging capacity based on electrogenerated chemiluminescence detection using tris(2,2'-bipyridine)ruthenium(III) by flow injection analysis.
We describe a new method for detecting hydroxyl radical scavenging capacity based on tris(2,2'-bipyridine)ruthenium(III) [Ru(bpy)(3)(3+)] chemiluminescence and flow injection analysis. Hydroxyl radicals were generated by the Fenton reaction. The scavenging capacity of the six antioxidants tested decreased in the following order: edaravone>L-tryptophan>gallic acid>Trolox>N-acetyl-L-cysteine>ascorbic acid. The proposed method allowed a sample throughput of about 80 samples/h. The six antioxidants were found to inhibit chemiluminescence intensity of Ru(bpy)(3)(2+). The proposed method is a rapid, selective, and accurate procedure for the study of hydroxyl radical scavenging capacity by Ru(bpy)(3)(3+) chemiluminescence.
Nobushi
Yasuhito
Y
College of Pharmacy, Nihon University, Chiba, Japan.
Uchikura
Kazuo
K
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
D016166:Free Radical Scavengers; D009942:Organometallic Compounds; C058901:tris(2,2'-bipyridine)ruthenium III; D017665:Hydroxyl Radical; D015082:2,2'-Dipyridyl