[Synthesis and identification of artificial antigen to the common moiety of some organophosphorus pesticides].
OBJECTIVE
To develop an immunoassay for multiple residues of organophosphorus pesticides.


METHODS
The generic hapten O, O-dimethyl-O-(4-carboxyphenyl)ester (HP) was synthesized by the reaction between O, O-Dimethyl phosphorochloridothionate and 4-hydroxybenzoic acid methyl ester, and then conjugated to bovine serum albumin (BSA) by active ester method for immunogens and ovalbumin (OVA) by mixed anhydride reaction for coating antigens.


RESULTS
The hapten was synthesized successfully, which was characterized by MS. The coupling ratios were 15.6:1 (HP-BSA) and 16.2:1(HP-OVA), respectively, calculated by UV spectrophotometry.


CONCLUSIONS
suggested that the artificial antigens could be used for production of broad specificity anti-organophosphorous pesticides antibody.
Jiang
Panpan
P
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. jiangpan_19841204@163.com
Chen
Chen
C
Li
Zhaohui
Z
He
Jiguo
J
D000941:Antigens / Q000737:chemistry*; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000276:immunology; D010047:Ovalbumin / Q000737:chemistry; D010575:Pesticides / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000941:Antigens; D006241:Haptens; D009943:Organophosphorus Compounds; D010575:Pesticides; D012710:Serum Albumin, Bovine; D010047:Ovalbumin