title: Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques.
abstract: A combination of high-throughput material formulation and microarray techniques were synergistically applied for the efficient analysis of the biological functionality of 135 binary polymer blends. This allowed the identification of cell-compatible biopolymers permissive for human skeletal stem cell growth in both in vitro and in vivo applications. The blended polymeric materials were developed from commercially available, inexpensive and well characterised biodegradable polymers, which on their own lacked both the structural requirements of a scaffold material and, critically, the ability to facilitate cell growth. Blends identified here proved excellent templates for cell attachment, and in addition, a number of blends displayed remarkable bone-like architecture and facilitated bone regeneration by providing 3D biomimetic scaffolds for skeletal cell growth and osteogenic differentiation. This study demonstrates a unique strategy to generate and identify innovative materials with widespread application in cell biology as well as offering a new reparative platform strategy applicable to skeletal tissues.
authors.affiliation: School of Chemistry, The University of Edinburgh, Kings Buildings, West Mains Road, EH9 3JJ, UK.
authors.forename: Ferdous
authors.initials: F
authors.lastname: Khan
authors.forename: Rahul S
authors.initials: RS
authors.lastname: Tare
authors.forename: Janos M
authors.initials: JM
authors.lastname: Kanczler
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005269:Femur / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers