Agrocybe chaxingu polysaccharide prevent inflammation through the inhibition of COX-2 and NO production.
The inhibition of nitric oxide (NO) and cyclooxygenase-2 (COX-2) production is considered to be a promising approach to the treatment of various diseases, including inflammation and cancer. In this study, we examined the effects of the Agrocybe chaxingu beta-glucan (polysaccharide) on lipopolysaccaride (LPS)-induced nitric oxide (NO) and cyclooxygenase-2 (COX-2) expression in murine macrophage Raw 264.7 cells as well as 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema in mice. The polysaccharide significantly inhibited (P 0.01) LPS-induced iNOS and COX-2 expression levels in the cells. Furthermore, topical application of polysaccharide resulted in markedly inhibited (P 0.01) TPA-induced ear edema in mice. These results suggest that this polysaccharide may be used for NO- and COX-2-related disorders such as inflammation and cancer.
Lee
Byung Ryong
BR
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, Korea.
Kim
So Young
SY
Kim
Dae Won
DW
An
Jae Jin
JJ
Song
Ha Yong
HY
Yoo
Ki Yeon
KY
Kang
Tae Cheon
TC
Won
Moo Ho
MH
Lee
Kwang Jae
KJ
Kim
Kwang Hee
KH
Joo
Jin Ho
JH
Ham
Hun Ju
HJ
Hur
Jang Hyun
JH
Cho
Sung Woo
SW
Han
Kyu Hyung
KH
Lee
Kil Soo
KS
Park
Jinseu
J
Choi
Soo Young
SY
Eum
Won Sik
WS
D055362:Agrocybe / Q000737:chemistry*; D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000276:immunology; D007249:Inflammation / Q000517:prevention & control*; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*
D011134:Polysaccharides; D009569:Nitric Oxide; D051546:Cyclooxygenase 2