Effects of sevoflurane on collagen production and growth factor expression in rats with an excision wound.
BACKGROUND
Sevoflurane is a widely used inhalation anesthetic, but there are no studies on its effect on the wound-healing process. This study was undertaken to evaluate the effect of exposure time to sevoflurane on wound healing.


METHODS
Male Sprague-Dawley rats were used. Two circular full-thickness skin defects 8 mm in diameter were made on the dorsum of the rats. The animals were divided into six groups according to exposed gas type and time: S1 (sevoflurane, 1 h), S4 (sevoflurane, 4 h), S8 (sevoflurane, 8 h), O1 (oxygen, 1 h), O4 (oxygen, 4 h), and O8 (oxygen, 8 h). The surface area of the wounds was measured 0, 1, 3, and 7 days after surgery. Separately, the mean blood pressures (MBP) and arterial oxygen pressures (PaO(2)) were monitored during the sevoflurane exposure. Collagen type I production and transforming growth factor-beta1 (TGF-beta1) and basic fibroblast growth factor (bFGF) expression on the wound surface were analyzed. Routine histological analysis was also performed.


RESULTS
Exposure duration to sevoflurane had no influence on MBP and PaO(2). The reduction in wound size and collagen type I production was delayed in S8. The expression of TGF-beta1 and bFGF on the wound surface in S8 was significantly attenuated in S8. The histology of the S8 demonstrated a delayed healing status.


CONCLUSIONS
Prolonged exposure to sevoflurane might alter the inflammatory phase of the wound-healing process by attenuation of growth factor expression such as TGF-beta1 and bFGF and subsequently by reduced collagen production.
Lee
H-J
HJ
Department of Anesthesia and Pain Medicine, Pusan National University, Busan, Korea.
Kwon
J-Y
JY
Shin
S-W
SW
Baek
S-H
SH
Choi
K-U
KU
Jeon
Y-H
YH
Kim
W-S
WS
Bae
J-H
JH
Choi
H-J
HJ
Kim
H-K
HK
Baik
S-W
SW
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D015153:Blotting, Western; D003094:Collagen / Q000096:biosynthesis*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D010100:Oxygen / Q000097:blood; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000378:metabolism* / Q000473:pathology
D018685:Anesthetics, Inhalation; D036341:Intercellular Signaling Peptides and Proteins; D008738:Methyl Ethers; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2; D000077149:Sevoflurane; D012313:RNA; D003094:Collagen; D010100:Oxygen