title: Sex- and tissue-specific effects of waterborne estrogen on estrogen receptor subtypes and E2-mediated gene expression in the reproductive axis of goldfish.
abstract: This research examined the gene expression profile of three goldfish estrogen receptor (ER) subtypes in multiple tissues in relation to mRNA levels of aromatase B and vitellogenin (VTG) following waterborne estrogen exposures. The protocol consisted of: i) adult male goldfish in late gonadal recrudescence exposed to 1 nM 17beta-estradiol (E2); ii) adult male and female goldfish in early sexual regression exposed to 1 nM E2 for 3, 6, 12 and 24h; and, iii) sexually mature, adult male goldfish exposed to 0.3 nM 17alpha-ethynylestradiol (EE2) for 24h. Liver produced the most consistent response with up-regulation of ERalpha in sexually regressed, mature and recrudescing males and in sexually regressed females. The dose and length of exposure, reproductive state and sex affected the auto-regulation of ERbeta1 by E2. ERbeta2 was not affected in any experiments suggesting it may not be auto-regulated by E2. Aromatase B and VTG gene expression were affected by E2, but also by other experimental conditions. EE2 induced liver ERalpha and VTG mRNA levels indicating that high environmental EE2 levels induce E2-mediated gene expression in a model teleost. These studies reveal a more complicated action of estrogenic compounds that has important implications on estrogenic endocrine disruptors in teleosts.
authors.affiliation: Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada. marlatt@isamundo.org
authors.forename: Vicki L
authors.initials: VL
authors.lastname: Marlatt
authors.forename: Josh
authors.initials: J
authors.lastname: Lakoff
authors.forename: Kate
authors.initials: K
authors.lastname: Crump
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Martyniuk
authors.forename: Jennifer
authors.initials: J
authors.lastname: Watt
authors.forename: Linda
authors.initials: L
authors.lastname: Jewell
authors.forename: Susanna
authors.initials: S
authors.lastname: Atkinson
authors.forename: Jules M
authors.initials: JM
authors.lastname: Blais
authors.forename: Jim
authors.initials: J
authors.lastname: Sherry
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Moon
authors.forename: Vance L
authors.initials: VL
authors.lastname: Trudeau
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics; D004958:Estradiol / Q000378:metabolism; D045166:Estradiol Congeners / Q000633:toxicity*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D004997:Ethinyl Estradiol / Q000633:toxicity; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006054:Goldfish / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects / Q000235:genetics; D012727:Sex Characteristics; D014018:Tissue Distribution; D014819:Vitellogenins / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D045166:Estradiol Congeners; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; D004958:Estradiol; D001141:Aromatase