title: [A rapid colorimetric assay for viability of osteosarcoma cells after chemotherapy].
abstract: OBJECTIVE
  To introduce a rapid colorimetric method for assessing the viability of osteosarcoma cells after chemotherapy.
  
  
  METHODS
  Colorimetric assay and automatic microplate scanning spectrophotometer were used for assaying the viability of osteosarcoma cells.
  
  
  RESULTS
  Close correlation was found between the absorbance at 570 nm of the formazan products and the number of viable osteosarcoma cells.
  
  
  CONCLUSIONS
  An effective, sensitive and convenient colorimetric assay has been established to assess the survival of osteosarcoma cells following chemotherapy.
authors.affiliation: Department of Spinal and Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. cqnfyy@yahoo.com.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Jian-ting
authors.initials: JT
authors.lastname: Chen
authors.forename: Zhan-jun
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Deng
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry / Q000379:methods; D005562:Formazans; D006801:Humans; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology*; D012680:Sensitivity and Specificity; D013778:Tetrazolium Salts
chemical_list: D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan