Single-step bioconversion for the preparation of L-gulose and L-galactose.
Both carbohydrate monomers L-gulose and L-galactose are rarely found in nature, but are of great importance in pharmacy R&D and manufacturing. A method for the production of L-gulose and L-galactose is described that utilizes recombinant Escherichia coli harboring a unique mannitol dehydrogenase. The recombinant E. coli system was optimized by genetic manipulation and directed evolution of the recombinant protein to improve conversion. The resulting production process requires a single step, represents the first readily scalable system for the production of these sugars, is environmentally friendly, and utilizes inexpensive reagents, while producing L-galactose at 4.6 g L(-1)d(-1) and L-gulose at 0.90 g L(-1)d(-1).
Woodyer
Ryan D
RD
zuChem, Inc., 2225 W. Harrison., Suite F, Chicago, IL 60612, USA. rwoodyer@zuchem.com
Christ
Trevor N
TN
Deweese
Kara A
KA
D000077102:Apium / Q000201:enzymology; D057005:Bioengineering / Q000191:economics / Q000379:methods; D004795:Enzyme Stability; D004926:Escherichia coli / Q000378:metabolism; D004376:Galactitol / Q000378:metabolism; D005690:Galactose / Q000096:biosynthesis*; D006601:Hexoses / Q000096:biosynthesis*; D007700:Kinetics; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism; D013237:Stereoisomerism
D006601:Hexoses; D011994:Recombinant Proteins; D004376:Galactitol; C057406:gulose; D013012:Sorbitol; D008354:Mannitol Dehydrogenases; D005690:Galactose