title: Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their anti-invasive activity on HT-29 human colon cancer cells.
abstract: We isolated anthocyanins from fruits of Vitis coignetiae Pulliat, characterized the anthocyanin profile, and investigated the anti-invasive effects of the anthocyanins on human colon cancer cells. The anthocyanins inhibited cell invasion in a dose-dependent manner, as measured by Matrigel invasion assays, by suppression of matrix metalloproteinase (MMP)-2 and MMP-9 expression. The anti-invasive activity of the anthocyanins was associated with modulation of constitutive nuclear factor kappaB (NF-kappaB) activation. The activation of NF-kappaB triggered by tumor necrosis factor-alpha was also inhibited by the anthocyanins through suppression IkappaBalpha phosphorylation. AIMs inhibited the expression of NF-kappaB-regulated proteins. In conclusion, this study suggested that the anthocyanins isolated from fruits of V. coignetiae Pulliat should have anti-invasive activities on human colon cancer cells and the activities should be related to the inhibition of NF-kappaB-regulated proteins such as MMP-2 and MMP-9 expression through the inhibition of NF-kappaB activation.
authors.affiliation: Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, South Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Yun
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jing Nan
authors.initials: JN
authors.lastname: Lu
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Kang
authors.forename: Hoon Gu
authors.initials: HG
authors.lastname: Kim
authors.forename: Dong Chul
authors.initials: DC
authors.lastname: Kim
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Choi
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Hae Gyeong
authors.initials: HG
authors.lastname: Kim
authors.forename: Yun-Kyoung
authors.initials: YK
authors.lastname: Lee
authors.forename: Chung Ho
authors.initials: CH
authors.lastname: Ryu
authors.forename: Gonsup
authors.initials: G
authors.lastname: Kim
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Ock Jin
authors.initials: OJ
authors.lastname: Park
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D015153:Blotting, Western; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D007150:Immunohistochemistry; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D009361:Neoplasm Invasiveness; D021241:Spectrometry, Mass, Electrospray Ionization; D014162:Transfection; D027843:Vitis / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D008156:Luciferases; D051550:I-kappa B Kinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9