Carbondioxide pneumoperitoneum prevents postoperative adhesion formation in a rat cecal abrasion model.
OBJECTIVE
We aimed to investigate the role of the carbon-dioxide (CO(2)) pneumoperitoneum on the prevention of postoperative adhesion formation.


METHODS
Thirty adult male Sprague-Dawley rats (250-350 g) were used for this study. The rats were randomly divided into three groups; in the sham group (n = 10) only, laparotomy was made, in group 2 (n = 10), a pneumoperitoneum was applied for a half-hour duration, followed by laparatomy, and in group 3 (n = 10), pneumoperitoenum time was 2 hours and after a pneumoperitoneum laparotomy was made. A cecal abrasion model was studied for making an adhesion formation. On day 14, all rats were sacrificed and adhesions were scored. Tissue samples from adhesions and peritonea and the cecum wall were examined, both pathologically and biochemically, for tissue hydroxyproline content.


RESULTS
There was a statistically significant difference between the control and pneumoperitoneum groups, regarding adhesion numbers and grades (P < 0.001). Cecal tissue hydroxypyroline content level was lower in group 1, and statistically significant differences were observed between groups 1, 2, and 3 regarding hydroxyproline content (P < 0.001). There was a statistically significant difference between the control and pneumoperitoneum groups regarding inflammation (P = 0.002).


CONCLUSIONS
Pretreatment with the CO(2) pneumoperitoneum had a prophylactic effect on postoperative adhesion formation in this study.
Kece
Can
C
Gastroenterologic Surgery, Yuksek Ihtisas Hospital, Ankara, Turkey. cankece@gmail.com
Ulas
Murat
M
Ozer
Ilter
I
Ozel
Ummuhani
U
Bilgehan
Ayse
A
Aydog
Gulden
G
Dalgic
Tahsin
T
Oymaci
Erkan
E
Bostanci
Birol
B
D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage; D002429:Cecal Diseases / Q000517:prevention & control*; D008297:Male; D010532:Peritoneal Diseases / Q000517:prevention & control*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000517:prevention & control
D002245:Carbon Dioxide