Raman spectroscopy of Xenopus laevis oocytes.
This work reports on the application of Raman spectroscopy for the analysis of Xenopus laevis oocytes (stage-I). A two-color home-made microscope has been used for this investigation. In particular, a 785nm Raman probe has been used to acquire the spontaneous Raman scattering from the oocyte cytoplasm, while a 532nm probe has been employed to detect carotenoids through Resonant Raman Scattering. Finally, the distribution of beta-carotene along a diameter of a single oocyte has been investigated.
Rusciano
Giulia
G
University of Naples Federico II, Department of Physics, via Cinthia, Napoli, Italy.
Pesce
Giuseppe
G
Salemme
Marinella
M
Selvaggi
Lara
L
Vaccaro
Carmen
C
Sasso
Antonio
A
Carotenuto
Rosa
R
D000465:Algorithms; D000818:Animals; D002338:Carotenoids / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D004867:Equipment Design; D005260:Female; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods; D009865:Oocytes / Q000378:metabolism*; D010777:Photochemistry / Q000379:methods; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014982:Xenopus laevis / Q000378:metabolism*
D002338:Carotenoids