Guidelines for the use of band-selective radiofrequency pulses in hetero-nuclear NMR: example of longitudinal-relaxation-enhanced BEST-type 1H-15N correlation experiments.
Band-selective radiofrequency (rf) pulses provide powerful spectroscopic tools for many biomolecular NMR applications. Band-selectivity is commonly achieved by pulse shaping where the rf amplitude and phase are modulated according to a numerically optimized function. This results in complex spin evolution trajectories during the pulse duration. Here we introduce simplified representations of a number of shaped pulses, commonly used in biomolecular NMR spectroscopy. These simple schemes, consisting in a suite of free evolution delays and ideal rf pulses, reproduce astonishingly well the effect on a scalar-coupled hetero-nuclear two-spin system. As a consequence, optimal use of such pulse shapes in complex multi-pulse sequences becomes straightforward, as demonstrated here for the example of longitudinal-relaxation-enhanced BEST-HSQC and BEST-TROSY experiments. Applications of these optimized pulse sequences to several proteins in the size range of 8-21 kDa are shown.
Lescop
Ewen
E
Centre de Recherche CNRS de Gif-sur-Yvette, Institut de Chimie des Substances Naturelles, Laboratoire de Chimie et Biologie Structurales, 1, avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Kern
Thomas
T
Brutscher
Bernhard
B
D000465:Algorithms; D004574:Electromagnetic Fields; D017408:Guidelines as Topic; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D012984:Software
D009587:Nitrogen Isotopes; D011522:Protons