title: Use of ATR-FTIR microspectroscopy to monitor autolysis of Saccharomyces cerevisiae cells in a base wine.
abstract: In this study, we evaluated the potentialities of ATR-FTIR microspectroscopy coupled to PCA in monitoring the major biochemical changes that occur during the autolysis of yeasts used for sparkling wine production. For this purpose, mid-infrared measurements were made on cells of the model strain Saccharomyces cerevisiae EC1118 in the course of autolysis induced at 30 degrees C for five days in a model and in a base wine. By relating principal component loadings to the corresponding absorption bands, it was shown that they well describe compositional modifications induced by autolytic process on yeast cells, such as partial hydrolysis of proteins, increase of peptides, free nucleotides, lipids, mannans, and beta-1,3 glucans. The corresponding score-score plots allowed us to monitor the different kinetics and to distinguish among faster, intermediate, and slower processes. ATR-FTIR microspectroscopy coupled with PCA is proposed as a sensitive method that can provide useful information to select efficient yeast strains, capable of accelerated autolysis, to be used in the second fermentation and aging of sparkling wines.
authors.affiliation: Dipartimento di Scienze, Tecnologie e Mercati della Vite e del Vino, Universita degli Studi di Verona, Via della Pieve 70, 37029 San Floriano, Verona, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Cavagna
authors.forename: Rossana
authors.initials: R
authors.lastname: Dell'Anna
authors.forename: Francesca
authors.initials: F
authors.lastname: Monti
authors.forename: Franca
authors.initials: F
authors.lastname: Rossi
authors.forename: Sandra
authors.initials: S
authors.lastname: Torriani
mesh_terms: D005285:Fermentation; D007218:Industrial Microbiology; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014920:Wine / Q000382:microbiology*