title: Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway.
abstract: Geminiviruses include a large number of single-stranded DNA viruses that are emerging as useful tools to dissect many fundamental processes in plant hosts. However, there have been no reports yet regarding the genetic dissection of the geminivirus-plant interaction. Here, a high-throughput approach was developed to screen Arabidopsis activation-tagged mutants which are resistant to geminivirus Beet severe curly top virus (BSCTV) infection. A mutant, lsb1 (less susceptible to BSCTV 1), was identified, in which BSCTV replication was impaired and BSCTV infectivity was reduced. We found that the three genes closest to the T-DNA were up-regulated in lsb1, and the phenotypes of lsb1 could only be recapitulated by the overexpression of GDU3 (GLUTAMINE DUMPER 3), a gene implicated in amino acid transport. We further demonstrated that activation of LSB1/GDU3 increased the expression of components in the salicylic acid (SA) pathway, which is known to counter geminivirus infection, including the upstream regulator ACD6. These data indicate that up-regulation of LSB1/GDU3 affects BSCTV infection by activating the SA pathway. This study thus provides a new approach to study of the geminivirus-host interaction.
authors.affiliation: Stake Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 West Xin-Gang Road, Guangzhou 510275, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kunling
authors.initials: K
authors.lastname: Teng
authors.forename: Jianbin
authors.initials: J
authors.lastname: Lai
authors.forename: Yiyue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yiliang
authors.initials: Y
authors.lastname: Huang
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Liming
authors.initials: L
authors.lastname: Liang
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Wang
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
authors.forename: Huishan
authors.initials: H
authors.lastname: Guo
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial; D017791:Geminiviridae / Q000472:pathogenicity* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D016254:Mutagenesis, Insertional; D009154:Mutation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000821:virology; D020156:Salicylic Acid / Q000378:metabolism*; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C036483:T-DNA; D020156:Salicylic Acid