title: Electrogenerated chemiluminescence of blue emitting ZnSe quantum dots and its biosensing for hydrogen peroxide.
abstract: A novel method for the detection of hydrogen peroxide (H(2)O(2)) has been developed, based on electrogenerated chemiluminescence (ECL) of blue emitting ZnSe quantum dots (QDs) in aqueous solution. The weak ECL emission of ZnSe QDs was generated when the electrode potential was scanned from 0.0 to -1.4V (versus Ag/AgCl), and the ECL intensity was greatly enhanced in the presence of H(2)O(2) or dissolved oxygen. Under the optimal conditions, the dynamic range is from 6.10 x 10(-7) to 3.10 x 10(-4)M H(2)O(2), and the detection limit (at S/N=3) is 2.00 x 10(-7)M. The relative standard deviation (at 5.00 x 10(-5)M of H(2)O(2)) is 2.70% (n=11). A possible reaction mechanism of ECL of ZnSe QDs was also investigated. As a novel biosensor, it was used to detect H(2)O(2) in the pig kidney (pk-15) cell, vero cell and mineral water, respectively. Moreover, the proposed biosensor showed a good reproducibility and potential application in real samples.
authors.affiliation: College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Hu
authors.forename: Heyou
authors.initials: H
authors.lastname: Han
authors.forename: Lijuan
authors.initials: L
authors.lastname: Hua
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Sheng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003116:Color; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods; D004581:Electronics / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D018036:Selenium Compounds; D017967:Zinc Compounds; D006861:Hydrogen Peroxide; C044696:zinc selenide