A beta-glucan-conjugate vaccine and anti-beta-glucan antibodies are effective against murine vaginal candidiasis as assessed by a novel in vivo imaging technique.
The protective capacity of a parenterally administered beta-glucan-conjugate vaccine formulated with the human-compatible MF59 adjuvant was assessed in a murine model of vaginal candidiasis. To monitor infection, an in vivo imaging technique exploiting genetically engineered, luminescent Candida albicans was adopted, and compared with measurements of colony forming units. The vaccine conferred significant protection, and this was associated with production of serum and vaginal anti-beta-glucan IgG antibodies. Vaginal IgG molecules were the likely mediators of protection as inferred by the efficacy of passive transfer of immune vaginal fluid and passive protection by an anti-beta-1,3-glucan mAb. Overall, the in vivo imaging technique was more reliable than vaginal CFU counts in assessing the extent and duration of the vaginal infection, and the consequent protection level.
Pietrella
Donatella
D
Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
Rachini
Anna
A
Torosantucci
Antonella
A
Chiani
Paola
P
Brown
Alistair J P
AJ
Bistoni
Francesco
F
Costantino
Paolo
P
Mosci
Paolo
P
d'Enfert
Christophe
C
Rappuoli
Rino
R
Cassone
Antonio
A
Vecchiarelli
Anna
A
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D002181:Candidiasis, Vulvovaginal / Q000276:immunology / Q000517:prevention & control*; D015169:Colony Count, Microbial; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D011136:Polysorbates / Q000008:administration & dosage; D013185:Squalene / Q000008:administration & dosage; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology; D047071:beta-Glucans / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D005657:Fungal Vaccines; C089950:MF59 oil emulsion; D011136:Polysorbates; D018074:Vaccines, Conjugate; C048844:anti-IgG; D047071:beta-Glucans; D013185:Squalene; C033363:beta-1,3-glucan