Aminodextran containing magnetite nanoparticles for molecular biology applications: preparation and evaluation.
Within the framework of medical diagnosis, the main objective is the development of hydrophilic magnetic particles for the generic capture of the nucleic acids in order to enhance the sensitivity. The strategy used in this work is based on the synthesis of cationic and hydrophilic magnetic nanoparticles bearing aminodextran. The synthesis was performed using two different processes: (i) coprecipitation of the ferrous and ferric salts in the presence of an aqueous solution of aminodextran and (ii) via adsorption of aminodextran on iron oxide nanoparticles. The obtained particles are characterized and evaluated in non-specific nucleic acids extraction and amplification.
Mouaziz
Hanna
H
Université de Lyon, F-69622, Lyon, France.
Veyret
Raphaël
R
Theretz
Alain
A
Ginot
Frédéric
F
Elaissari
Abdelhamid
A
D003460:Crystallization / Q000379:methods*; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008967:Molecular Biology / Q000379:methods*; D008968:Molecular Conformation; D015336:Molecular Probe Techniques; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D013499:Surface Properties
D003911:Dextrans; D004337:Drug Carriers; D046911:Macromolecular Substances; D052203:Ferrosoferric Oxide