A multiparallel bioreactor for the cultivation of mammalian cells in a 3D-ceramic matrix.
For adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface-to-volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set-up the system together with handling procedures for long-time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices' caverns.
Goralczyk
Vicky
V
TU Berlin, Chair of Measurement and Control, Berlin, Germany.
Driemel
Gregor
G
Bischof
Andreas
A
Peter
Andrea
A
Berthold
Almuth
A
Kroh
Lothar
L
Blessing
Lucienne
L
Schubert
Helmut
H
King
Rudibert
R
D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D019149:Bioreactors; D016466:CHO Cells; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design; D004996:Ethidium / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose; D007785:Lactose; D008855:Microscopy, Electron, Scanning; D016062:Porosity
D005452:Fluoresceins; D005456:Fluorescent Dyes; D004996:Ethidium; D005947:Glucose; D007785:Lactose; D000537:Aluminum Oxide; C018506:diacetylfluorescein