title: Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC.
abstract: A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 x 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (mug/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets.
authors.affiliation: Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: D F
authors.initials: DF
authors.lastname: Tian
authors.forename: X L
authors.initials: XL
authors.lastname: Tian
authors.forename: T
authors.initials: T
authors.lastname: Tian
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: F K
authors.initials: FK
authors.lastname: Mo