Electrochemical detection in a paper-based separation device.
Prototypes of microfluidic paper-based separation devices with amperometric detection were developed and evaluated. Photolithography was used to make a gold electrochemical microcell on polyester and that microcell was coupled to a strip of paper where a chromatographic separation occurs. The device performance was demonstrated with the separation and quantification of uric and ascorbic acid in mixtures. The method provides an analytical alternative for the determination of compounds where low cost and simplicity are essential.
Carvalhal
Rafaela Fernanda
RF
Analytical Chemistry Department, Institute of Chemistry State University of Campinas-UNICAMP, SP, P.O. Box 6154, Campinas, Brazil.
Kfouri
Marta Simão
MS
Piazetta
Maria Helena de Oliveira
MH
Gobbi
Angelo Luiz
AL
Kubota
Lauro Tatsuo
LT
D001205:Ascorbic Acid / Q000032:analysis* / Q000302:isolation & purification; D002854:Chromatography, Paper / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008823:Micelles; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D014527:Uric Acid / Q000032:analysis* / Q000302:isolation & purification
D008823:Micelles; D014527:Uric Acid; D006046:Gold; D001205:Ascorbic Acid