title: Decreased NKCC1 activity in erythrocytes from African Americans with hypertension and dyslipidemia.
abstract: BACKGROUND
  Recent studies demonstrated a key role of ubiquitous isoform of Na+,K+,2Cl- co-transport (NKCC1) in regulation of myogenic tone and peripheral resistance. We examined the impact of race, gender, and plasma lipid on NKCC1 activity in French Canadians and African Americans with hypertension and dyslipidemia.
  
  
  METHODS
  NKCC and passive erythrocyte membrane permeability to K+, measured as ouabain-resistant, bumetanide-sensitive, and (ouabain+bumetanide)-resistant 86Rb influx, respectively, were compared in 111 French-Canadian men, 107 French-Canadian women, 26 African-American men, and 45 African-American women with essential hypertension and dyslipidemia.
  
  
  RESULTS
  The African-American men and women were 7 years younger and presented twofold decreased plasma triglycerides compared to their French-Canadian counterparts (P < 0.01) whereas body mass index (BMI), total cholesterol, low-density lipoprotein, and high-density lipoprotein (HDL) were not different. NKCC was respectively 50 and 38% lower in the African-American men and women than in the French Canadians (P < 0.005) without any differences in passive erythrocyte membrane permeability for K+. We did not observe any impact of age on NKCC in all groups under investigation, whereas plasma triglycerides correlated positively with the activity of this carrier in the French-Canadian men only.
  
  
  CONCLUSIONS
  NKCC1 activity is lower in erythrocytes of African Americans with essential hypertension and dyslipidemia than in Caucasian counterparts. We suggest that decreased NKCC1 may contribute to the feature of the pathogenesis of salt-sensitive hypertension seen in African Americans.
authors.affiliation: Research Centre, Centre hospitalier de l'Université de Montréal (CRCHUM)-Technopôle Angus, Montreal, Quebec, Canada. sergei.n.orlov@umontreal.ca
authors.forename: Sergei N
authors.initials: SN
authors.lastname: Orlov
authors.forename: Francis
authors.initials: F
authors.lastname: Gossard
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Pausova
authors.forename: Olga A
authors.initials: OA
authors.lastname: Akimova
authors.forename: Johanne
authors.initials: J
authors.lastname: Tremblay
authors.forename: Clarence E
authors.initials: CE
authors.lastname: Grim
authors.forename: Jane M
authors.initials: JM
authors.lastname: Kotchen
authors.forename: Theodore A
authors.initials: TA
authors.lastname: Kotchen
authors.forename: Daniel
authors.initials: D
authors.lastname: Gaudet
authors.forename: Allen W
authors.initials: AW
authors.lastname: Cowley
authors.forename: Pavel
authors.initials: P
authors.lastname: Hamet
mesh_terms: D000328:Adult; D001741:Black or African American; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015992:Body Mass Index; D050171:Dyslipidemias / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D051381:Rats; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism*; D064506:Solute Carrier Family 12, Member 2; D044465:White People; D055815:Young Adult
chemical_list: D008055:Lipids; C578877:SLC12A2 protein, human; C578879:Slc12a2 protein, rat; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2