Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids.
In this study, various fatty acids of 18 and 22 carbons were used as substrates to produce sophorolipids in Candida bombicola ATCC 22214. Methyl esterification of erucic acid and C22-enriched rapeseed oil generated mainly acidic sophorolipids with different degrees of saturation in the fatty acid chain, which was demonstrated by structural analysis using HPLC, LC-MS and NMR spectroscopy. Regardless of methyl esterification, the rapeseed oil served as the best substrate for high production of sophorolipids among the tested. Methyl esterification also had no noticeable effect on the interfacial properties of sophorolipids. However, 22 carbons introduced in the fatty acid chain increased the hydrophobicity of sophorolipids, and therefore improved surface-active properties and biodegradability.
Shin
Jae Dong
JD
National Research Lab of Bioactive Materials Lab, Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea.
Lee
Jeongmi
J
Kim
Yong Bum
YB
Han
In-Sun
IS
Kim
Eun-Ki
EK
D017738:Alkanesulfonic Acids / Q000378:metabolism; D001673:Biodegradation, Environmental / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003902:Detergents / Q000494:pharmacology; D004952:Esters / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D006017:Glycolipids / Q000096:biosynthesis*; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D010938:Plant Oils / Q000737:chemistry; D000074262:Rapeseed Oil; D012996:Solutions; D013501:Surface-Active Agents / Q000494:pharmacology
D017738:Alkanesulfonic Acids; D003902:Detergents; D004952:Esters; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D006017:Glycolipids; D008823:Micelles; D010938:Plant Oils; D000074262:Rapeseed Oil; D012996:Solutions; D013501:Surface-Active Agents; C030641:alkylbenzyl sulfonic acid; D002244:Carbon; D002266:Carboxymethylcellulose Sodium