Whole-body, real-time preclinical imaging of quantum dot fluorescence with time-gated detection.
We describe a wide-field preclinical imaging system optimized for time-gated detection of quantum dot fluorescence emission. As compared to continuous wave measurements, image contrast was substantially improved by suppression of short-lifetime background autofluorescence. Real-time (8 frames/s) biological imaging of subcutaneous quantum dot injections is demonstrated simultaneously in multiple living mice.
May
Andrzej
A
Bhaumik
Srabani
S
Gambhir
Sanjiv S
SS
Zhan
Chun
C
Yazdanfar
Siavash
S
D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D045663:Quantum Dots; D051598:Whole Body Imaging / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes