Use of inbred mouse strains to map recognition receptors of MCMV infected cells in the NK cell gene locus.
Genetically distinct inbred strains of mice that differ in their susceptibility to mouse cytomegalovirus (MCMV) are invaluable for dissecting complex host-pathogen interactions. Their study has allowed the identification of host-resistance loci, including several activating NK cell receptors of major histocompatibility complex (MHC) class I. In this chapter, we provide a practical guide to the genetic mapping and functional characterization of NK cell receptors that control innate immunity against MCMV via specific recognition of infected cells.
Fodil-Cornu
Nassima
N
Department of Human Genetics, McGill University, Montreal, QC, Canada.
Pyzik
Michal
M
Vidal
Silvia M
SM
D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D056426:Genetic Loci / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D016678:Genome / Q000235:genetics; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018146:Muromegalovirus / Q000502:physiology*; D010641:Phenotype; D011247:Pregnancy; D055607:Receptors, Natural Killer Cell / Q000235:genetics*; D013379:Substrate Specificity
D018076:DNA, Complementary; D005819:Genetic Markers; D055607:Receptors, Natural Killer Cell