Crystallization and preliminary X-ray crystallographic analysis of DNA gyrase GyrB subunit from Xanthomonas oryzae pv. oryzae.
DNA gyrase is a type II topoisomerase that is essential for chromosome segregation and cell division owing to its ability to modify the topological forms of bacterial DNA. In this study, the N-terminal fragment of the GyrB subunit of DNA gyrase from Xanthomonas oryzae pv. oryzae was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.10 A resolution using a synchrotron-radiation source. The crystal belonged to space group I4(1), with unit-cell parameters a = b = 110.27, c = 70.75 A. The asymmetric unit contained one molecule, with a V(M) of 2.57 A(3) Da(-1) and a solvent content of 50.2%.
Jung
Ha Yun
HY
Department of Chemistry, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Lee
Ki Jeung
KJ
Kim
Kyung Ha
KH
Hyoung
Ji Hye
JH
Han
Mi Ra
MR
Kim
Hyun Kyoung
HK
Kang
Lin-Woo
LW
Ahn
Yeh-Jin
YJ
Heo
Yong-Seok
YS
D003460:Crystallization; D018360:Crystallography, X-Ray; D027081:DNA Gyrase / Q000737:chemistry*; D014974:Xanthomonas
D027081:DNA Gyrase