title: Preparation of biomolecule microstructures and microarrays by thiol-ene photoimmobilization.
abstract: A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol-ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 microm in size covering an area of up to 75x25 mm were created. Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips.
authors.affiliation: Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Weinrich
authors.forename: Maja
authors.initials: M
authors.lastname: Köhn
authors.forename: Pascal
authors.initials: P
authors.lastname: Jonkheijm
authors.forename: Ulrika
authors.initials: U
authors.lastname: Westerlind
authors.forename: Leif
authors.initials: L
authors.lastname: Dehmelt
authors.forename: Hans
authors.initials: H
authors.lastname: Engelkamp
authors.forename: Peter C M
authors.initials: PC
authors.lastname: Christianen
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kuhlmann
authors.forename: Jan C
authors.initials: JC
authors.lastname: Maan
authors.forename: Dirk
authors.initials: D
authors.lastname: Nüsse
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schröder
authors.forename: Ron
authors.initials: R
authors.lastname: Wacker
authors.forename: Edgar
authors.initials: E
authors.lastname: Voges
authors.forename: Rolf
authors.initials: R
authors.lastname: Breinbauer
authors.forename: Horst
authors.initials: H
authors.lastname: Kunz
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D001710:Biotin / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008027:Light; D051379:Mice; D046228:Microarray Analysis; D018396:Mucin-1 / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry*; D055668:Photochemical Processes; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D006020:Glycopeptides; D055767:Immobilized Proteins; D018396:Mucin-1; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D001710:Biotin