Neonatal cerebral hypoxia-ischemia impairs plasticity in rat visual cortex.
Ocular dominance plasticity (ODP) following monocular deprivation (MD) is a model of activity-dependent neural plasticity that is restricted to an early critical period regulated by maturation of inhibition. Unique developmental plasticity mechanisms may improve outcomes following early brain injury. Our objective was to determine the effects of neonatal cerebral hypoxia-ischemia (HI) on ODP. The rationale extends from observations that neonatal HI results in death of subplate neurons, a transient population known to influence development of inhibition. In rodents subjected to neonatal HI and controls, maps of visual response were derived from optical imaging during the critical period for ODP and changes in the balance of eye-specific response following MD were measured. In controls, MD results in a shift of the ocular dominance index (ODI) from a baseline of 0.15 to -0.10 (p < 0.001). Neonatal HI with moderate cortical injury impairs this shift, ODI = 0.14 (p < 0.01). Plasticity was intact in animals with mild injury and in those exposed to hypoxia alone. Neonatal HI resulted in decreased parvalbumin expression in hemispheres receiving HI compared with hypoxia alone: 23.4 versus 35.0 cells/high-power field (p = 0.01), with no change in other markers of inhibitory or excitatory neurons. Despite abnormal inhibitory neuron phenotype, spontaneous activity of single units and development of orientation selective responses were intact following neonatal HI, while overall visual responses were reduced. Our data suggest that specific plasticity mechanisms are impaired following early brain injury and that the impairment is associated with altered inhibitory neuronal development and cortical activation.
Failor
Samuel
S
Department of Pediatrics, University of California San Francisco, San Francisco, California 94143, USA.
Nguyen
Vien
V
Darcy
Daniel P
DP
Cang
Jianhua
J
Wendland
Michael F
MF
Stryker
Michael P
MP
McQuillen
Patrick S
PS
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001931:Brain Mapping / Q000379:methods; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000503:physiopathology*; D009473:Neuronal Plasticity / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D020318:Rats, Long-Evans; D014793:Visual Cortex / Q000502:physiology*