miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARgamma expression.
microRNAs (miRNAs) are non-coding small RNAs regulating gene expression, cell growth, and differentiation. Although several miRNAs have been implicated in cell growth and differentiation, it is barely understood their roles in adipocyte differentiation. In the present study, we reveal that miR-27a is involved in adipocyte differentiation by binding to the PPARgamma 3'-UTR whose sequence motifs are highly conserved in mammals. During adipogenesis, the expression level of miR-27a was inversely correlated with that of adipogenic marker genes such as PPARgamma and adiponectin. In white adipose tissue, miR-27a was more abundantly expressed in stromal vascular cell fraction than in mature adipocyte fraction. Ectopic expression of miR-27a in 3T3-L1 pre-adipocytes repressed adipocyte differentiation by reducing PPARgamma expression. Interestingly, the level of miR-27a in mature adipocyte fraction of obese mice was down-regulated than that of lean mice. Together, these results suggest that miR-27a would suppress adipocyte differentiation through targeting PPARgamma and thereby down-regulation of miR-27a might be associated with adipose tissue dysregulation in obesity.
Kim
Sang Yun
SY
Institute of Molecular Biology & Genetics, Seoul National University, Kwanak-Gu, Seoul, Republic of Korea.
Kim
A Young
AY
Lee
Hyun Woo
HW
Son
You Hwa
YH
Lee
Gha Young
GY
Lee
Joo-Won
JW
Lee
Yun Sok
YS
Kim
Jae Bum
JB
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics*
D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D047495:PPAR gamma