title: Isolation of a novel gene encoding a 3,5,6-trichloro-2-pyridinol degrading enzyme from a cow rumen metagenomic library.
abstract: 3,5,6-trichloro-2-pyridinol (TCP) is a major metabolite of the insecticide chlorpyrifos and is hazardous to human and animal health. A gene encoding a TCP degrading enzyme was cloned from a metagenomic library prepared from cow rumen. The gene (tcp3A) is 2.5 kb in length, encoding a protein (Tcp3A) of 599 amino acid residues. Tcp3A has a potential signal sequence, as well as a putative ATP/GTP binding site, and a likely amidation site. The molecular weight of the enzyme is 62 kDa by SDS-PAGE. Comparison of Tcp3A with the NCBI database using BLASTP revealed homology to amidohydrolase proteins. Recombinant Escherichia coli harboring the tcp3A gene could utilize TCP as the sole source of carbon. TLC and HPLC revealed that TCP was degraded by recombinant E. coli harboring tcp3A. This is the first report of a gene encoding a TCP degrading enzyme.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, Chinju, 660-701, Republic of Korea.
authors.forename: Renukaradhya K
authors.initials: RK
authors.lastname: Math
authors.forename: Shah Md
authors.initials: SM
authors.lastname: Asraful Islam
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.forename: Sun Joo
authors.initials: SJ
authors.lastname: Hong
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Myoung Geun
authors.initials: MG
authors.lastname: Yun
authors.forename: Ji Joong
authors.initials: JJ
authors.lastname: Cho
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Heo
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Han Dae
authors.initials: HD
authors.lastname: Yun
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002417:Cattle; D003001:Cloning, Molecular; D007306:Insecticides / Q000378:metabolism; D056186:Metagenomics; D008969:Molecular Sequence Data; D011728:Pyridones / Q000378:metabolism*; D012417:Rumen / Q000737:chemistry* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; D011728:Pyridones; C012587:3,5,6-trichloro-2-pyridinol; D000581:Amidohydrolases