title: Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma.
abstract: BACKGROUND
  Oxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients.
  
  
  METHODS
  Endometrial tissues of gynecological patients with different diagnoses were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides.
  
  
  RESULTS
  Superoxide dismutase activity was significantly decreased (50% in average) in hyperplastic and adenocarcinoma patients. Activities of both glutathione peroxidase and glutathione reductase were increased 60% and 100% on average, in hyperplastic patients, while in adenocarcinoma patients only glutathione reductase activity was elevated 100%. Catalase activity was significantly decreased in adenocarcinoma patients (47%). Lipid hydroperoxides level was negatively correlated to superoxide dismutase and catalase activities, and positively correlated to glutathione peroxidase and glutathione reductase activities.
  
  
  CONCLUSIONS
  This study provided the first comparison of antioxidant status and lipid peroxidation in endometrial tissues of patients with polyps, myoma, hyperplasia and adenocarcinoma. The results showed that patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, although the extent of disturbance varied with the diagnosis. Further investigation is needed to clarify the mechanisms responsible for the observed alterations and whether lipid hydroperoxide levels and antioxidant enzyme activities in uterus of gynecological patients might be used as additional parameter in clinical evaluation of gynecological disorders.
authors.affiliation: Laboratory of Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, PO Box 522, 11001 Belgrade, Serbia. snezana@vinca.rs
authors.forename: Snezana
authors.initials: S
authors.lastname: Pejić
authors.forename: Ana
authors.initials: A
authors.lastname: Todorović
authors.forename: Vesna
authors.initials: V
authors.lastname: Stojiljković
authors.forename: Jelena
authors.initials: J
authors.lastname: Kasapović
authors.forename: Snezana B
authors.initials: SB
authors.lastname: Pajović
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism*; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000378:metabolism*; D004717:Endometrium / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D006965:Hyperplasia / Q000201:enzymology / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000502:physiology*; D008054:Lipid Peroxides / Q000378:metabolism; D008875:Middle Aged; D009214:Myoma / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D011127:Polyps / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase