Detection of chronic lymphocytic leukemia cell surface markers using surface enhanced Raman scattering gold nanoparticles.
Selective targeting and detection of a hematologic malignancy, chronic lymphocytic leukemia, using surface enhanced Raman scattering (SERS) gold nanoparticles is reported. The functional nanoparticles were composed of a gold core onto which an optical reporter dye was adsorbed, protected from aggregation by grafted polyethyleneglycol, and targeted to CD19 antigen by antibodies. The signals were detected by dark-field microscopy and Raman spectrometry. The observation that the Raman signals are not disrupted by several traditional pathology stains indicates advantages over fluorescence methods.
Nguyen
Collin T
CT
Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.
Nguyen
James T
JT
Rutledge
Steven
S
Zhang
Janing
J
Wang
Chen
C
Walker
Gilbert C
GC
D018941:Antigens, CD19 / Q000032:analysis*; D006046:Gold; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis*; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles; D036103:Nanotechnology; D013059:Spectrum Analysis, Raman / Q000379:methods*
D018941:Antigens, CD19; D006046:Gold