title: High mass selectivity for top-down proteomics by application of SWIFT technology.
abstract: Stored waveform inverse Fourier transform (SWIFT) technology has been implemented on a commercial Fourier transform ICR mass spectrometer. Complex ejection/isolation waveforms can be generated on an arbitrary waveform generator (AWG) and applied to the instrument by use of a high speed analog switch. High mass selectivity and subsequent electron capture dissociation (ECD) of the SWIFT isolated ions has been demonstrated with analysis of intact Bovine histone H4. A mass selectivity about 0.1 m/z unit for isolation of the 18+ charge state ions was achieved. Adaptation of SWIFT on the commercial instrument provides an enhanced capability for characterizing intact proteins by top-down analysis.
authors.affiliation: Mass Spectrometry Facility and Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158-2517, USA. sguan@cgl.ucsf.edu
authors.forename: Shenheng
authors.initials: S
authors.lastname: Guan
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010449:Peptide Mapping / Q000295:instrumentation*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods