Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells.
Sixteen heat-killed bifidobacteria isolated from human intestine and a probiotic strain Lactobacillus GG were tested for their ability to influence IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells in vitro. The bifidobacteria suppressed IgE-mediated degranulation of RBL-2H3 cells by 1.6-56.4% in a strain-dependent manner. Bifidobacteria from healthy infants expressed high inhibitory effects on IgE-mediated degranulation (41-55%), while those from allergic infants varied greatly in their effects against degranulation. Bifidobacteria taxonomically identified as Bifidobacterium bifidum exhibited much stronger inhibitory effects against IgE-mediated degranulation than those taxonomically identified as B. adolescentis (P < 0.05).These results indicate that the intestinal bifidobacteria might be one of factors influencing IgE-mediated allergic responses.
Harata
Gaku
G
Technical Research Laboratory, Takanashi Milk Products Co. Ltd., 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023, Japan.
He
Fang
F
Takahashi
Kyoko
K
Hosono
Akira
A
Kawase
Manabu
M
Kubota
Akira
A
Hiramatsu
Masaru
M
Kaminogawa
Shuichi
S
D000818:Animals; D015549:Basophil Degranulation Test; D001491:Basophils / Q000276:immunology*; D001644:Bifidobacterium / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007422:Intestines / Q000382:microbiology; D007778:Lactobacillus / Q000502:physiology; D015471:Leukemia, Basophilic, Acute / Q000276:immunology* / Q000473:pathology; D051381:Rats; D015398:Signal Transduction
D007073:Immunoglobulin E