[Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene].
OBJECTIVE
To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.


METHODS
A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.


RESULTS
Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.


CONCLUSIONS
BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
Yin
Wen
W
Center Laboratory, Fourth Medical Military University, Xi'an 710032, China. yinwen@fmmu.edu.cn
Lü
Xin
X
Lei
Ying-feng
YF
Yang
Jing
J
Sun
Meng-ning
MN
Ma
Yu
Y
Lan
Hai-yun
HY
Wang
Ai-qin
AQ
D000818:Animals; D001500:BCG Vaccine / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000097:blood; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
D001500:BCG Vaccine; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C050806:presurface protein 1, hepatitis B surface antigen