title: Non-apoptotic cell death induced by nutritional starvation in J774.1 mouse macrophage-like cell line.
abstract: The growth and amino acid utilization of a mouse macrophage-like cell line J774.1 was investigated in two different culture media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine, was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3 activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines, and DMEM is superior to RPMI1640 for long-term cell culture.
authors.affiliation: MPL, Division of Pharmacology, Meikai University School of Dentistry, Saitama 350-0283, Japan.
authors.forename: May Maw
authors.initials: MM
authors.lastname: Thet
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yokote
authors.forename: Qing
authors.initials: Q
authors.lastname: Chu
authors.forename: Qin-Tao
authors.initials: QT
authors.lastname: Wang
authors.forename: Kaori
authors.initials: K
authors.lastname: Kishino
authors.forename: Nozomi
authors.initials: N
authors.lastname: Tamura
authors.forename: Jun
authors.initials: J
authors.lastname: Shimada
authors.forename: Osamu
authors.initials: O
authors.lastname: Amano
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kanda
authors.forename: Shiro
authors.initials: S
authors.lastname: Kunii
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Takekawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Hatano
authors.forename: Chiyako
authors.initials: C
authors.lastname: Shimada
authors.forename: Hideo
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death; D002478:Cells, Cultured; D004789:Enzyme Activation; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D013217:Starvation
chemical_list: D000596:Amino Acids; D053148:Caspase 3