Metabonomics study of urine from Sprague-Dawley rats exposed to Huang-yao-zi using (1)H NMR spectroscopy.
Urinary metabolic perturbations associated with liver toxicity induced by Huang-yao-zi (root of Dioscorea bulifera L.) were studied using nuclear magnetic resonance spectroscopy ((1)H NMR) to determine the correlations between metabonomic profiling and histopathologic/biochemical observations and to discover biomarkers for liver toxicity. Huang-yao-zi with a maximal tolerance dose (MTD) was given to male Sprague-Dawley rats for 72h followed by metabonomic analysis of urine samples collected at 24 and 72h. The results revealed that the levels of taurine, creatine, betaine, dimethylglycine (DMG), acetate, glycine were elevated, whereas, the levels of succinate, 2-oxoglutarate, citrate, hippurate and urea were reduced. Partial least square (PLS)-discrimination analysis (DA) of NMR spectra revealed two apparent clusters between control groups and treatment groups, indicating metabolic changes observed in urine samples in response to Huang-yao-zi treatment. In addition, mechanism associated with oxidative injury of hepatic mitochondria was investigated. These results indicated that (1)H NMR-based metabonomics analysis in urine samples may be useful for predicting hepatotoxicity induced by Huang-yao-zi.
Liu
Yanru
Y
Institute of Radiation Medicine, Academy of Military Medical Sciences, No. 27, Taiping Rd., Haidian District, Beijing 100850, PR China.
Huang
Rongqing
R
Liu
Lijun
L
Peng
Jiangnan
J
Xiao
Bingkun
B
Yang
Jianyun
J
Miao
Zhenchun
Z
Huang
Honglin
H
D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000652:urine; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000473:pathology / Q000652:urine*; D016002:Discriminant Analysis; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism*; D016018:Least-Squares Analysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D020714:Maximum Tolerated Dose; D055432:Metabolomics / Q000379:methods*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D016482:Urinalysis
D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C539630:huangyaozi