Mechanisms regulating invasiveness and growth of endometriosis lesions in rat experimental model and in humans.
OBJECTIVE
To compare the expression of MMP-2, TIMP-2, and TGFbeta2 mRNA in experimental and human endometriotic lesions and to assess the possibility of its cytokine regulation.


METHODS
Experimental laboratory study.


METHODS
Medical center. ANIMALS AND PATIENT(S): Thirty female Wistar rats, 17 women with endometriosis, 11 healthy women.


METHODS
Uterine transplants were attached to rat peritoneum via the surgical autotransplantation technique. The collection of endometriotic implants at 7, 14, and 21 days postsurgery and laparoscopic collection of peritoneal fluid, ectopic, and matched eutopic endometrium from women with endometriosis were performed.


METHODS
MMP-2, TIMP-2, TGFbeta2 mRNA expression in endometrium was assessed by real-time reverse-transcription polymerase chain reaction.


RESULTS
In rats, the increase of MMP-2 and decrease of TIMP-2 mRNA expression was noted at the 7th day, and an increase of TGFbeta2 mRNA expression was seen at the 14th day postsurgery. In humans, elevation of TIMP-2 mRNA expression in eutopic endometrium and of MMP-2, TGFbeta2 mRNA expression in ectopic endometrium was observed. Autologous peritoneal fluid stimulated MMP-2 mRNA expression in eutopic endometrium of women with endometriosis. Cytokines derived from ectopic lesions mononuclear cells increased TGFbeta2 mRNA expression in endometrium of healthy women.


CONCLUSIONS
Supposedly MMP-TIMP balance is important in promoting endometriotic tissue invasion and TGFbeta2 in regulating ectopic endometrium growth.
Sotnikova
Natalia Yu
NY
Department of Clinical Immunology, Research State Institute of Maternity and Childhood, Ivanovo, Russia.
Antsiferova
Yulia S
YS
Posiseeva
Lyubov V
LV
Shishkov
Dmitrii N
DN
Posiseev
Denis V
DV
Filippova
Ekaterina S
ES
D000328:Adult; D000818:Animals; D001202:Ascitic Fluid / Q000502:physiology; D004195:Disease Models, Animal; D004715:Endometriosis / Q000209:etiology / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis* / Q000235:genetics; D053781:Transforming Growth Factor beta2 / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D053781:Transforming Growth Factor beta2; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2