Novel folate binding protein-1 interactions in embryonic orofacial tissue.
OBJECTIVE
To identify proteins with which FolBp1 may interact within lipid rafts in tissue derived from embryonic orofacial tissue.


METHODS
A yeast two-hybrid screen of a cDNA library, derived from orofacial tissue from gestational day 11 to 13 mouse embryos, was conducted.


RESULTS
Using the full-length FolBp1 protein as bait, two proteins that bind FolBp1 were identified, Bat2d, and a fibronectin type III-containing domain protein. Results were confirmed by glutathione S-transferase pull-down assays.


CONCLUSIONS
As a component of membrane lipid raft protein complexes, these binding proteins may represent "helper" or chaperone proteins that associate with FolBp1 in order to facilitate the transport of folate across the plasma membrane. The protein-protein interactions detected, while limited in number, may be critical in mediating the role of FolBp1 in folate transport, particularly in the developing embryo.
Pisano
M Michele
MM
University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, ULSD, Louisville, KY 40292, United States.
Bhattacherjee
Vasker
V
Wong
Leeyean
L
Finnell
Richard H
RH
Greene
Robert M
RM
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000235:genetics / Q000378:metabolism*; D021962:Membrane Microdomains / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D005492:Folic Acid