title: High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia.
abstract: Self-renewal is a feature of cancer and can be assessed by cell transplantation into immune-compromised or immune-matched animals. However, studies in zebrafish have been severely limited by lack of these reagents. Here, Myc-induced T-cell acute lymphoblastic leukemias (T-ALLs) have been made in syngeneic, clonal zebrafish and can be transplanted into sibling animals without the need for immune suppression. These studies show that self-renewing cells are abundant in T-ALL and comprise 0.1% to 15.9% of the T-ALL mass. Large-scale single-cell transplantation experiments established that T-ALLs can be initiated from a single cell and that leukemias exhibit wide differences in tumor-initiating potential. T-ALLs also can be introduced into clonal-outcrossed animals, and T-ALLs arising in mixed genetic backgrounds can be transplanted into clonal recipients without the need for major histocompatibility complex matching. Finally, high-throughput imaging methods are described that allow large numbers of fluorescent transgenic animals to be imaged simultaneously, facilitating the rapid screening of engrafted animals. Our experiments highlight the large numbers of zebrafish that can be experimentally assessed by cell transplantation and establish new high-throughput methods to functionally interrogate gene pathways involved in cancer self-renewal.
authors.affiliation: Huntsman Cancer Institute, Salt Lake City, UT, USA.
authors.forename: Alexandra C H
authors.initials: AC
authors.lastname: Smith
authors.forename: Aubrey R
authors.initials: AR
authors.lastname: Raimondi
authors.forename: Chris D
authors.initials: CD
authors.lastname: Salthouse
authors.forename: Myron S
authors.initials: MS
authors.lastname: Ignatius
authors.forename: Jessica S
authors.initials: JS
authors.lastname: Blackburn
authors.forename: Igor V
authors.initials: IV
authors.lastname: Mizgirev
authors.forename: Narie Y
authors.initials: NY
authors.lastname: Storer
authors.forename: Jill L O
authors.initials: JL
authors.lastname: de Jong
authors.forename: Aye T
authors.initials: AT
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Sergei
authors.initials: S
authors.lastname: Revskoy
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: David M
authors.initials: DM
authors.lastname: Langenau
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002469:Cell Separation; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation / Q000379:methods*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology*; D015027:Zebrafish / Q000235:genetics*