Peroxidase activity of DNA aptamer-pt complexes prepared with cisplatin.
DNA aptamers carrying Pt nanoparticles prepared with cisplatin showed peroxidase enzymatic activity while retaining the specific binding ability of the aptamers. Optimal preparation conditions of DNA-Pt complex prepared with cisplatin were investigated on the synthesis at pH 7-11, a reaction time of 1-18 h and 90 degrees C. The enzymatic reaction of DNA-Pt complex obeyed Michaelis-Menten kinetics. K(M) for the DNA-Pt complex was found to be of the same order as K(M) for hemin and hemin-DNA complex, but one order of magnitude higher than that of horseradish peroxidase. A sandwich type of DNA enzyme-linked aptamer assay (DLAA) using DNA-Pt complex successively detected target protein of thrombin. DLAA using DNA-Pt complex fractioned by ultrafiltration membranes having a molecular weight cut-off of 30 000 and 300 000 showed 1.9-times higher sensitivity than DLAA using DNA-Pt complex without fraction. The DNA-Pt complex having specific size was effective for the sensitive detection of thrombin in DLAA.
Higuchi
Akon
A
Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan. higuchi@ncu.edu.tw
Yang
Siou-Ting
ST
Siao
Yi-Di
YD
Hsieh
Pei-Vin
PV
Fukushima
Hisashi
H
Chang
Yung
Y
Chen
Wen-Yih
WY
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002945:Cisplatin / Q000737:chemistry; D006801:Humans; D009195:Peroxidase / Q000378:metabolism; D017671:Platinum Compounds / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D017671:Platinum Compounds; D009195:Peroxidase; D013917:Thrombin; D002945:Cisplatin