[The effect of recombinant HSP47siRNA on the pathological scar in nude mice: in vivo study].
OBJECTIVE
To study the significance of HSP47 gene in the development of pathological scar.


METHODS
The nude mice were used to reconstruct animal model of pathological scar. 16 days later, the mixture of recombinant HSP47siRNA and liposome was injected into the pathological scar in experimental group. In the control group, 0.25 ml PBS was injected intraperitoneally. 7 days after injection, the specimens were collected for detection of mRNA of HSP47, the collagen and for immunohistochemical study.


RESULTS
In the control and experimental group, the collagen content was (91.71 +/- 1.24)% and (82.12 +/- 4.79)%, respectively; the expression of HSP47mRNA was 1 042 862.01 +/- 604 194.36 and 306 123.68 +/- 105 857.08, respectively; the expression of collagen I mRNA was 10 228 614.70 +/- 2 532 879.04 and 6 011 841.97 +/- 2 886 897.17, respectively;the scar volume was (255.60 +/- 21.34) mm3 and (132.99 +/- 24.06) mm3, respectively. All the above results showed significant difference between the two groups (P < 0.05).


CONCLUSIONS
The collagen production can be reduced through suppression of the expression of HSP47 gene. It indicates that HSP47 gene enhance the development of keloid and could be used as the target of treatment.
Jin
Pei-Sheng
PS
The Affilliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Chen
Jun-Jie
JJ
Cen
Ying
Y
Zhang
Ai-Jun
AJ
Tao
Chang-Bo
CB
Li
Xue-Yang
XY
D000818:Animals; D002921:Cicatrix / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D003094:Collagen / Q000096:biosynthesis; D015316:Genetic Therapy; D005822:Genetic Vectors; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics / Q000627:therapeutic use*; D008081:Liposomes / Q000627:therapeutic use; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000627:therapeutic use
D050958:HSP47 Heat-Shock Proteins; D008081:Liposomes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D003094:Collagen