title: Rare human IFNG variants.
abstract: After the identification of the human interferon-gamma (IFNG) variant G54D (c.287G>A, ss105106770) in DNA samples from Ghana, West Africa, systematic mutation screening of IFNG by the LightCycler((R))-based procedure of high-resolution melting (HRM) revealed additional rare mutations. All variants occurred heterozygously only and were confirmed either by their detection in other individuals and/or by repeated DNA sequencing of independent PCR products.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Medicine, Hamburg, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Thye
authors.forename: Christa
authors.initials: C
authors.lastname: Ehmen
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Intemann
authors.forename: Margaret Amanua
authors.initials: MA
authors.lastname: Chinbuah
authors.forename: Andreas
authors.initials: A
authors.lastname: Ziegler
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Horstmann
authors.forename: Christian G
authors.initials: CG
authors.lastname: Meyer
mesh_terms: D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D009691:Nucleic Acid Denaturation; D012984:Software
chemical_list: D007371:Interferon-gamma