Granulomatous transformation of capillary lesions in pulmonary-renal syndrome autologously induced anti-glomerular basement membrane disease in Wistar-Kyoto rats.
BACKGROUND
Pulmonary-renal syndrome is characterized by pulmonary hemorrhage and rapidly progressive glomerulonephritis in various immunological states. Histopathological analysis of pulmonary-renal syndrome is not yet complete.


METHODS
Wistar-Kyoto (WKY) rats were sensitized using the noncollagenous (NC1) domain of type IV collagen from bovine kidney as an antigen. Histopathology of the kidneys and lungs was investigated with light microscopy, immunohistochemistry and electromicroscopy. Expression levels of cytokine mRNA were determined by real-time RT-PCR using renal tissue of rats.


RESULTS
Macrophage-rich granulomatous glomerulonephritis and alveolar capillaritis accompanied with pulmonary hemorrhage were induced by the sensitization. The humoral antibody against NC1 was detected on the glomerular and alveolar capillary walls. Th2 cytokine IL-10 was dominant over Th1 cytokine IFN-gamma in renal tissues of WKY rats.


CONCLUSIONS
The granulomatous transformation seemed to be induced by macrophage conspicuous capillaritis under dominant cellular immune reactions in WKY rats. In addition to Th1 cytokines, Th2 cytokines may also participate in the formation of granulomatous lesions.
Aoyagi
Daiju
D
Department of Pathology, Shinshu University School of Medicine, Matsumoto 390-8621, Japan.
Nakazawa
Koh
K
Kaneyama
Tomoki
T
Masumoto
Junya
J
Otani
Masako
M
Shigematsu
Hidekazu
H
D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000473:pathology*; D005921:Glomerulonephritis / Q000473:pathology*; D006099:Granuloma / Q000473:pathology*; D006470:Hemorrhage / Q000473:pathology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000473:pathology*; D007678:Kidney Glomerulus / Q000473:pathology; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000473:pathology*; D051381:Rats; D011921:Rats, Inbred WKY; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma