Isolation of mitochondria for biogenetical studies: An update.
The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.
Fernández-Vizarra
Erika
E
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
Ferrín
Gustavo
G
Pérez-Martos
Acisclo
A
Fernández-Silva
Patricio
P
Zeviani
Massimo
M
Enríquez
José Antonio
JA
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats