Ultrasound-induced membrane lipid peroxidation and cell damage of Escherichia coli in the presence of non-woven TiO2 fabrics.
A non-woven titanium dioxide (TiO(2)) fabric was applied to disinfection by ultrasound (US) irradiation, and the disinfection efficiency and lipid peroxidation of Escherichia coli (E. coli) cell membrane were evaluated to investigate the killing process. The addition of non-woven TiO(2) fabric enhanced hydroxyl (OH) radical generation and disinfection efficiency. Judging from the disinfection experiments using glutathione or t-butanol as a radical scavenger, the OH radical played a major role in cell killing in sonodynamic disinfection with non-woven TiO(2) fabric. Moreover, to understand the detailed killing process, damage to cell membrane was also evaluated using a diphenyl-1-pyrenylphosphine (DPPP) fluorescent probe, which detects the membrane's lipid peroxidation. The addition of non-woven TiO(2) fabric aggravated this peroxidation. This aggravation was caused by the OH radical according to an assay using a radical scavenger. From these results, it was concluded that non-woven TiO(2) fabric as a sonocatalyst promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced a major disorder in the E. coli cell membrane under US irradiation.
Rahman
Mohammad Mizanur
MM
Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Ninomiya
Kazuaki
K
Ogino
Chiaki
C
Shimizu
Nobuaki
N
D002384:Catalysis; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004203:Disinfection; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008563:Membrane Lipids / Q000378:metabolism*; D055668:Photochemical Processes; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D014465:Ultrasonics
D008563:Membrane Lipids; C009495:titanium dioxide; D017665:Hydroxyl Radical; D014025:Titanium