Enhanced multicolor resonant-optical-assay with direct text output.
The effectiveness of a novel multicolor biochip boosted by reducing cluster repulsion and in-situ silver enhancement has been demonstrated by using anti-serum albumine antibodies conjugated to gold nanoparticles on a vacuum metalized plastic film coated with nano-resonance driving ceramic multilayers. A dense and smooth vacuum deposited SiO approximately 1.6 top layer (50 to 300 nm thick) on a flexible thermoplastic polymer poly-ethylene-terephthalate-chip functionalized via poly-ethylenimine monolayer coating and chemical cross-linking was employed to immobilize capture antibodies. Following capturing of the human serum albumin antigen from analyte solution, the multicolor chip reacts with anti HSA-gold nanoparticles binding them in a nanometric distance to the resonant mirror of the device-visible to the eye as faint coloring of the chip surface. Following silver enhancement, a strong metallic angle-dependent color via Resonance Enhanced Absorption is observed. In this study, silver staining has been used for the first time to boost and shift the color of nano-resonance enhanced optical bioassays. The use of silver staining increases and significantly modifies the intensity of the resonance color and was done in less than 5 minutes directly on the chip. This novel methodology will find broad application in Point-of-Care diagnostic devices via a color signal output designed as a written text with high contrast to replace standard lateral flow devices just showing lines or dots.
Assadollahi
Saied
S
Attophotonics Biosciences GmbH, Austria.
Palkovits
Roland
R
Haglmüller
Jakob
J
Pointl
Peter
P
Schalkhammer
Thomas
T
D000419:Albuminuria / Q000175:diagnosis* / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation*; D055096:Optical Devices; D011933:Reagent Kits, Diagnostic; D012709:Serum Albumin; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D016482:Urinalysis / Q000295:instrumentation*
D011933:Reagent Kits, Diagnostic; D012709:Serum Albumin