Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris.
The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 microM<MIC<2.5 microM), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram (-) bacteria, Vibrio sp. (40 microM<MIC<80 microM). However, rLs-Stylicin1 is able to agglutinate Vibrio penaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (K(d)) of 9.6x10(-8)M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins.
Rolland
J L
JL
IFREMER, Université Montpellier 2, Centre National de la Recherche Scientifique, IRD, UMR 5119 Ecosystèmes Lagunaires, Montpellier, France. jean.luc.rolland@ifremer.fr
Abdelouahab
M
M
Dupont
J
J
Lefevre
F
F
Bachère
E
E
Romestand
B
B
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D001483:Base Sequence; D001681:Biological Assay; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D033561:Penaeidae / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000187:drug effects / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D011994:Recombinant Proteins