In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.
The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34(+) hematopoietic precursor cells into mature KIR(+) human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.
Cichocki
Frank
F
University of Minnesota Cancer Center, Minneapolis, MN, USA.
Miller
Jeffrey S
JS
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D054340:Receptors, KIR / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology; D013997:Time Factors; D014161:Transduction, Genetic
D018952:Antigens, CD34; D054340:Receptors, KIR