title: Temperature dependence of the sodium pump is altered in the cerebral cortex of CCK2 receptor-deficient mice.
abstract: Previously we have shown that the temperature dependence of the sodium pump (Na(+),K(+)-ATPase) is altered under different neuropathological conditions. In this study we compared temperature dependence of the Na(+),K(+)-ATPase in the fronto-parietal cortex of CCK(2) receptor-deficient (homo- and heterozygous) and normal (wild-type) mice. The Arrhenius plot for Na(+),K(+)-ATPase from wild-type brain is non-linear with a breakpoint at 20.3 +/- 0.4 degrees C. In case of the brain cell membrane of CCK(2) receptor-deficient mice (homo- and heterozygous) the breakpoint on Arrhenius plot was detected at 26.0 +/- 1.1 degrees C and 25.4 +/- 0.4 degrees C, respectively. The shift of the breakpoint on the Arrhenius plot established in CCK(2) receptor-deficiency as well as in case of some other pathological conditions confirms that such kind of alteration in the Na(+),K(+)-ATPase temperature dependence is likely related to the homeostatic adjustment of altered function of the sodium pump.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tartu University, Ravila 19, Tartu, Estonia. tiit.salum@kliinikum.ee
authors.forename: T
authors.initials: T
authors.lastname: Salum
authors.forename: S
authors.initials: S
authors.lastname: Kõks
authors.forename: C
authors.initials: C
authors.lastname: Kairane
authors.forename: R
authors.initials: R
authors.lastname: Mahlapuu
authors.forename: M
authors.initials: M
authors.lastname: Zilmer
authors.forename: E
authors.initials: E
authors.lastname: Vasar
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D006579:Heterozygote; D006720:Homozygote; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043887:Receptor, Cholecystokinin B / Q000172:deficiency*; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000502:physiology*; D013696:Temperature
chemical_list: D043887:Receptor, Cholecystokinin B; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase