title: Determination of para red, Sudan dyes, canthaxanthin, and astaxanthin in animal feeds using UPLC.
abstract: A simple high-performance liquid chromatography method was developed for quantitative determination of para red, Sudan I, Sudan II, Sudan III, Sudan IV, canthaxanthin, and astaxanthin in feedstuff. The sample was extracted using acetonitrile and cleaned up on a C(18) SPE column. The residues were analyzed using ultra-performance liquid chromatography coupled to a diode array detector at 500 nm. The mobile phase was acetonitrile-formic acid-water with a gradient elution condition. The external standard curves were calibrated. The mean recoveries of the seven colorants were 62.7-91.0% with relative standard deviation 2.6-10.4% (intra-day) and 4.0-13.2% (inter-day). The detection limits were in the range of 0.006-0.02 mg/kg.
authors.affiliation: Department of Animal Science, Beijing University of Agriculture, Beijing, PR China, 102206.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Hou
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.forename: Guojuan
authors.initials: G
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Miao
authors.initials: M
authors.lastname: Hong
authors.forename: Yongnin
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D001391:Azo Compounds / Q000032:analysis*; D016644:Canthaxanthin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005505:Food Coloring Agents / Q000032:analysis*; D057230:Limit of Detection; D009284:Naphthols / Q000032:analysis; D024341:Xanthophylls / Q000032:analysis
chemical_list: D001391:Azo Compounds; D005505:Food Coloring Agents; D009284:Naphthols; D024341:Xanthophylls; C024336:1-phenylazo-2-naphthol; D016644:Canthaxanthin; C033003:sudan red; C005948:astaxanthine; C009213:Scarlet Red; C033006:sudan III