Production and purification of low calcium response protein H of Chlamydophila pneumoniae.
Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein.
Faludi
Ildikó
I
Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary. faludi.ildiko@gmail.com
Csanádi
Agnes
A
Szabó
Agnes Míra
AM
Burián
Katalin
K
Endrész
Valéria
V
Miczák
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D023521:Chlamydophila Infections / Q000276:immunology / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins