Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli.
The effects of nine probiotic strains of Lactobacillus, Bifidobacterium, and Enterococcus on the growth, adhesion activity, and biofilm formation of enteroaggregative Escherichia coli (EAggEC) were examined. The culture supernatant of the E. faecium strain, with or without pH adjustment to a neutral pH, had a strong bactericidal effect on EAggEC, including induction of membrane damage and cell lysis. Supernatants of the L. casei ss. casei and L. casei ss. rhamnosus strains also had a bactericidal effect on EAggEC, but this activity was abolished by pH adjustment to a neutral pH. No inhibitory effect of the culture supernatants of Bifidobacterium or E. faecalis strains was detected. Adhesion of EAggEC to intestinal epithelial cells was not inhibited by the bacterial strains tested. Two strains of L. casei enhanced EAggEC biofilm formation, which was characterized by increased bacterial proliferation. These results suggest that the three different bacterial species; Lactobacillus, Bifidobacterium, and Enterococcus, have different effects on EAggEC, and that further analysis is required for the practical use of these bacteria as probiotics against EAggEC infection.
Miyazaki
Yoshibumi
Y
Department of Emergency and Critical Care Medicine, Nippon Medical School, Tokyo, Japan.
Kamiya
Shigeru
S
Hanawa
Tomoko
T
Fukuda
Minoru
M
Kawakami
Hayato
H
Takahashi
Hidemi
H
Yokota
Hiroyuki
H
D000898:Antibiosis; D001422:Bacterial Adhesion / Q000502:physiology; D001644:Bifidobacterium / Q000145:classification / Q000254:growth & development*; D018441:Biofilms / Q000254:growth & development; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D016983:Enterococcus / Q000145:classification / Q000254:growth & development*; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development* / Q000502:physiology*; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology; D007778:Lactobacillus / Q000145:classification / Q000254:growth & development*; D019936:Probiotics / Q000494:pharmacology*; D013045:Species Specificity