title: [RAPD analysis and construction of specific DNA probes between Pinellia ternata and Typhonium flagelliforme].
abstract: OBJECTIVE
  To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme.
  
  
  METHODS
  Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes and then conducted Southern blot.
  
  
  RESULTS
  A great deal of special bands was found between Pinellia ternata and Typhonium flagelliforme. A Pinellia ternata specific molecule was screened.
  
  
  CONCLUSIONS
  RAPD analysis and specific DNA probes show potential value in the identification of Pinellia ternata and Typhonium flagelliforme.
authors.affiliation: Institute of Immunology & Molecular Biology, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.forename: Quan
authors.initials: Q
authors.lastname: Bai
authors.forename: Chao-li
authors.initials: CL
authors.lastname: Wang
authors.forename: Bi-xuan
authors.initials: BX
authors.lastname: Ren
authors.forename: En-jie
authors.initials: EJ
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Bao-qian
authors.initials: BQ
authors.lastname: Jing
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Hu
mesh_terms: D002681:China; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D030021:Pinellia / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D018744:DNA, Plant