title: Crystallization and preliminary crystallographic analysis of the measles virus hemagglutinin in complex with the CD46 receptor.
abstract: The measles virus (MV) hemagglutinin (MV-H) mediates the attachment of MV particles to cell-surface receptors for entry into host cells. MV uses two receptors for attachment to host cells: the complement-control protein CD46 and the signalling lymphocyte activation molecule (SLAM). The MV-H glycoprotein from an Edmonston MV variant and the MV-binding fragment of the CD46 receptor were overproduced in mammalian cells and used to crystallize an MV-H-CD46 complex. Well diffracting crystals containing two complexes in the asymmetric unit were obtained and the structure of the complex was solved by the molecular-replacement method.
authors.affiliation: Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma, 28049 Madrid, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Santiago
authors.forename: Angel
authors.initials: A
authors.lastname: Gutiérrez-Rodríguez
authors.forename: Paul A
authors.initials: PA
authors.lastname: Tucker
authors.forename: Thilo
authors.initials: T
authors.lastname: Stehle
authors.forename: José M
authors.initials: JM
authors.lastname: Casasnovas
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006389:Hemagglutinins, Viral / Q000737:chemistry*; D006801:Humans; D008459:Measles virus; D051916:Membrane Cofactor Protein / Q000737:chemistry*; D011991:Receptors, Virus / Q000737:chemistry*
chemical_list: D006389:Hemagglutinins, Viral; D051916:Membrane Cofactor Protein; D011991:Receptors, Virus