Site-specific labeling of 'second generation' annexin V with 99mTc(CO)3 for improved imaging of apoptosis in vivo.
In this study 'second generation' AnxV was specifically labeled with (99m)Tc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged 'second generation' AnxV labeled with (99m)Tc(CO)(3) in comparison to (99m)Tc-HYNIC-cys-AnxV and (99m)Tc(CO)(3)-DTPA-cys-AnxV.
De Saint-Hubert
Marijke
M
Department of Nuclear Medicine, University Hospital Gasthuisberg Leuven, Herestraat 49, B-3000 Leuven, Belgium.
Mottaghy
Felix M
FM
Vunckx
Kathleen
K
Nuyts
Johan
J
Fonge
Humphrey
H
Prinsen
Kristof
K
Stroobants
Sigrid
S
Mortelmans
Luc
L
Deckers
Niko
N
Hofstra
Leo
L
Reutelingsperger
Chris P M
CP
Verbruggen
Alfons
A
Rattat
Dirk
D
D000818:Animals; D017304:Annexin A5 / Q000737:chemistry; D017209:Apoptosis; D022781:Hepatocytes / Q000166:cytology; D051379:Mice; D013667:Technetium / Q000737:chemistry; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
D017304:Annexin A5; D013667:Technetium