Vesicles with charged domains.
This work summarizes results obtained on membranes composed of the ternary mixture dioleoylphosphatidylglycerol (DOPG), egg sphingomyelin (eSM) and cholesterol (Chol). The membrane phase state as a function of composition is characterized from data collected with fluorescence microscopy on giant unilamellar vesicles. The results suggest that the presence of the charged DOPG significantly decreases the composition region of coexistence of liquid ordered and liquid disordered phases as compared to that in the ternary mixture of dioleoylphosphatidycholine, sphingomyelin and cholesterol. The addition of calcium chloride to DOPG:eSM:Chol vesicles, and to a lesser extent the addition of sodium chloride, leads to the stabilization of the two-phase coexistence region, which is expressed in an increase in the miscibility temperature. On the other hand, addition of the chelating agent EDTA has the opposite effect, suggesting that impurities of divalent cations in preparations of giant vesicles contribute to the stabilization of charged domains. We also explore the behavior of these membranes in the presence of extruded unilamellar vesicles made of the positively charged lipid dioleoyltrimethylammoniumpropane (DOTAP). The latter can induce domain formation in DOPG:eSM:Chol vesicles with initial composition in the one-phase region.
Vequi-Suplicy
Cíntia C
CC
Instituto de Física, Universidade de São Paulo, CP 66318 CEP 05315-970, São Paulo, Brazil.
Riske
Karin A
KA
Knorr
Roland L
RL
Dimova
Rumiana
R
D002122:Calcium Chloride / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry*; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D044367:Phase Transition
D008563:Membrane Lipids; D008567:Membranes, Artificial; D004492:Edetic Acid; D002122:Calcium Chloride