In vitro determination of tetanus toxicity by an endopeptidase assay linked to a ganglioside-binding step.
Assays for the detection of tetanus neurotoxin (TeNT) are relevant for research applications as well as for the safety testing of tetanus vaccines. So far, these assays are usually performed as toxicity tests in guinea pigs or mice. The alternative methods described to date were mostly based on the detection of the toxin's proteolytic activity. However, these endopeptidase assays turned out to be unreliable because they only measure the enzymatic activity as sole determinant of tetanus toxicity, while not taking into account other parameters like the toxin's capacity to bind to target cells. In order to better reflect the in vivo situation of a tetanus infection, we have linked an endopeptidase assay to a ganglioside-binding step. The resulting method, which offers a unique combination of two functionally linked assays, detects those TeNT molecules only which possess both a functional binding domain as well as an active enzymatic domain. Our results demonstrate that this assay is able to reliably detect TeNT, and therefore might provide a basis for the replacement of the animal tests for detection of tetanus toxicity. Moreover, the assay concept could also be useful for in vitro toxicity measurements of other toxins with similar subunit structures.
Behrensdorf-Nicol
Heike A
HA
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany. behhe@pei.de
Bonifas
Ursula
U
Kegel
Birgit
B
Silberbach
Katja
K
Krämer
Beate
B
Weisser
Karin
K
D000818:Animals; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D007202:Indicators and Reagents; D051379:Mice; D050683:R-SNARE Proteins / Q000378:metabolism; D013744:Tetanus Toxin / Q000633:toxicity*; D013745:Tetanus Toxoid / Q000633:toxicity
D005732:Gangliosides; D007202:Indicators and Reagents; D050683:R-SNARE Proteins; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; C016481:trisialoganglioside GT1; D010450:Endopeptidases