title: Relationship between endothelin-1 and interleukin-1beta in inflamed periodontal tissues.
abstract: We recently demonstrated that endothelin-1 (ET-1) was strongly expressed in inflamed gingival tissues, but the biological role of ET-1 in gingival tissue remains unknown. This study focused on the relationship between ET-1 and interleukin-1beta (IL-1beta), an important cytokine during the periodontal inflammatory process. We determined the protein levels of ET-1 and IL-1beta in gingival tissues from patients and examined whether ET-1 could regulate the expression of the IL-1beta gene and protein in oral epithelial cells and fibroblasts in vitro. There was a significant correlation between the levels of ET-1 and IL-1beta in 26 gingival tissues, as determined by ELISA. Following the confirmation of two specific ET-1 receptors (ETA and ETB receptors) on the cultured cells, the effects of ET-1 stimulation on IL-1beta mRNA and protein expression were evaluated by RT-PCR and ELISA, respectively. The IL-1beta mRNA and protein levels were enhanced by ET-1 stimulation in a dose-dependent manner, and the enhancement of IL-1beta was inhibited by ETA or ETB receptor antagonists. These findings indicate that ET-1 is involved in the regulation of IL-1beta expression in gingival tissues and suggest that ET-1 signaling to the cells may be a therapeutic target for treating IL-1beta-dependent inflammatory responses.
authors.affiliation: Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Rikimaru
authors.forename: Shuji
authors.initials: S
authors.lastname: Awano
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Mineoka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshida
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ansai
authors.forename: Tadamichi
authors.initials: T
authors.lastname: Takehara
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D065130:Endothelin A Receptor Antagonists; D065131:Endothelin B Receptor Antagonists; D019332:Endothelin-1 / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D055815:Young Adult
chemical_list: D065130:Endothelin A Receptor Antagonists; D065131:Endothelin B Receptor Antagonists; D019332:Endothelin-1; D053583:Interleukin-1beta