[Serum proteomics profiling for diagnosis of myasthenia gravis].
OBJECTIVE
To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis.


METHODS
The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The differentially expressed protein peaks were identified to establish a MG diagnostic model. And preliminary validation was performed.


RESULTS
Thirty-eight specific protein peaks with significant differences were found in the serum protein pattern of 56 MG patients and 16 healthy controls. Systemic optimization identified 2 protein peaks of M4168.94 and M1122.57. And they were used to build the MG diagnostic model of differentiating 56 cases from 16 controls.


CONCLUSIONS
The serum protein profiling can be a novel, effective and sensitive tool to screen for MG-related protein peaks and establish a diagnostic model.
Wu
Guo-Yong
GY
Department of Cardiothoracic Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. guoyongwu2003@yahoo.com
Pang
Jing-Zhuo
JZ
Cheng
Chao
C
Lu
Jian-Jun
JJ
Ma
Jun
J
Gu
Yong
Y
Zhong
Fu-Tian
FT
Luo
Hong-He
HH
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009157:Myasthenia Gravis / Q000097:blood / Q000175:diagnosis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult
D001798:Blood Proteins