[Extracts of mucopolysaccharides from Asterias rollestoni and its immunoregulation effect in vitro].
OBJECTIVE
To study immunoregulation effects on normal pulpal cells of the mucopolysaccharides MP1 and MP2 extracted from Asterias rollestoni body wall and viscera, respectively.


METHODS
The orthogonal design was employed to obtain the best possible combination of the critical parameters for mucopolysaccharide MP2. MP1, MP2, glycosides, MP1 + glycosides and MP2 + glycosides were added respectively into pulpal cells culture media to test their effects on spleen cells proliferations by MTT assay.


RESULTS
MP1 and MP2 could certainly increase the proliferation of the normal splenocyte and inhibit glycosides cytotoxicity. Compared with controls, MP2 reversed the cytotoxicity of glycosides at concentration of 25 to 40 mg/mL, the greatest activity appearing at the dose of 40 mg/mL (P<0.01), while MP1 didn't reverse it.


CONCLUSIONS
MP1 and MP2 can enhance immunity of normal mice, and MP2 can significantly reverses the cytotoxicity of glycosides in vitro.
Xu
Fa-long
FL
Marine College of Shandong University in Weihai, Weihai 264209, China. xfl_19@yahoo.cn
Li
Yu-qiang
YQ
Li
Fei-fei
FF
Yan
Ke-qin
KQ
Zhang
Feng
F
Chen
Shen-xi
SX
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D047271:Asterias / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006025:Glycosaminoglycans / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000008:administration & dosage / Q000633:toxicity; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D000276:Adjuvants, Immunologic; D006025:Glycosaminoglycans; D012503:Saponins