[Immune regulation effect of rat dendritic cells phagocytosing photochemotherapy-treated allogeneic cells on syngeneic T cells].
The aim of this study was to investigate the immune regulatory effect of dendritic cells phagocytosing photochemotherapy-treated allogeneic spleen lymphocytes on syngeneic T cells. DA rat spleen lymphocytes were treated with 8-methoxypsoralen plus UVA irradiation (PUVA). LEW rat bone marrow-derived DCs were co-cultured with PUVA-treated DA spleen lymphocytes (PUVA-SP), and the surface markers (MHC-II, CD86 and CD40) of treated DC were detected by flow cytometry. CFSE-labeled PUVA SP were incubated with LEW DCs and the phagocytosis of DCs on PUVA-SP was observed by using fluorescent microscope. The ability of DC phagocytosing allogeneic PUVA-SP (PUVA-SP DC) to stimulate the proliferation of LEW T cells was analyzed by mixed leukocyte reactions (MLR). The production of IL-4, IL-10, IL-2, IFN-gamma in MLR culture supernatant was determined by luminex method. The results indicated that the PUVA treatment effectively induced early apoptosis of DA rat spleen lymphocytes. After co-culture, DC efficiently phagocytosed allogeneic PUVA-SP and still maintained an immature phenotype with low levels of MHC II, CD40 and CD86. PUVA-SP DC induced LEW T cell hyporesponsiveness to DA rat antigen, and led to skewing of T cell cytokine expression toward Th2 (IL-10 and IL-4). It is concluded that the PUVA-SP DC effectively down-regulate T cell response to alloantigen and induce Th2 immune deviation in vitro.
Zheng
De-Hua
DH
Chinese PLA Transplant Center, The Second Affiliated Hospital, Chinese PLA General Hospital, Beijing 100091, China.
Wei
Yu-Xiang
YX
Shi
Bing-Yi
BY
Zou
Yi-Ping
YP
DU
Guo-Sheng
GS
Zhu
Zhi-Dong
ZD
Song
Ji-Yong
JY
Shi
Ying-Chang
YC
Li
Li
L
D000818:Animals; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology*; D005434:Flow Cytometry; D007519:Isoantigens; D010587:Phagocytosis / Q000276:immunology*; D010777:Photochemistry; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*
D007519:Isoantigens