A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus.
A tumor necrosis factor (TNF) gene has been isolated and characterized in kuruma shrimp, Marsupenaeus japonicus, providing the first conclusive evidence for the existence of the TNF ligand in shrimp. The kuruma shrimp TNF (MjTNF) cDNA was composed of 1868 bp with a 262 bp 5'-untranslated region (UTR) and a 220 bp 3'-UTR, which was translated into a protein of 462 amino acid residues that included a predicted transmembrane domain of 23 amino acid residues (Trp20-Val42) and the TNF family signature (Pro321-Leu448). Homology analysis of MjTNF showed 30.7% and 26.7% identities with fruit fly (Drosophila melanogaster) Eiger and human (Homo sapiens) ectodysplasin A, respectively. The MjTNF gene was constitutively expressed in unstimulated organs of shrimp such as the muscle, stomach, brain and gill. In lymphoid organ cells, an enhanced expression of the MjTNF gene was observed following stimulation with peptidoglycan and polycytidylic acid. A high expression level of MjTNF was observed in vivo 2 h and 4 h after stimulation with lipopolysaccharide and Vibrio penaeicida, respectively. These observations suggest that MjTNF plays a role in the innate immune defense in kuruma shrimp. The discovery of shrimp TNF will allow a more complete and concrete understanding of shrimp inflammatory responses.
Mekata
Tohru
T
Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi, 889-2192 Miyazaki, Japan.
Sudhakaran
Raja
R
Okugawa
Shogo
S
Inada
Mari
M
Kono
Tomoya
T
Sakai
Masahiro
M
Itami
Toshiaki
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D010457:Peptidoglycan / Q000494:pharmacology; D010802:Phylogeny; D011066:Poly C / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology*; D014733:Vibrio / Q000276:immunology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D014409:Tumor Necrosis Factor-alpha; D011066:Poly C