The application of fully automated on-line solid phase extraction in bioanalysis.
This paper describes the application of fully automated on-line solid phase extraction to the bioanalysis of three example compounds using the Symbiosis platform. The on-line assay performance is compared to off-line methodologies for the same compounds. The three example compounds possess a variety of physicochemical properties and different extraction modes were applied in off-line methods. These methods were developed through optimisation of solid phase or liquid-liquid extraction and chromatographic separation conditions for each of the analytes. Both on-line and off-line methods were evaluated for linearity, carryover, imprecision and inaccuracy. Experiments were also performed investigating modification of ionisation and selectivity against different batches of plasma. On-line and off-line methods were found to be comparable in performance. In conclusion, on-line methodology has distinct advantages for the analysis of large numbers of samples with a marked reduction in manual operation.
Mitchell
Richard J
RJ
Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent CT13 9NJ, UK. richard.j.mitchell@pfizer.com
Christian
Rachelle
R
Hughes
Heather
H
Miah
Asadh
A
Walker
Donald K
DK
D057205:Automation, Laboratory; D056148:Chromatography, Reverse-Phase; D015725:Fluconazole / Q000097:blood; D006801:Humans; D007189:Indans / Q000097:blood; D013058:Mass Spectrometry; D009862:Online Systems; D011422:Propionates / Q000097:blood; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D007189:Indans; D011422:Propionates; D015725:Fluconazole; C062766:candoxatril