title: In situ patrolling of regulatory T cells is essential for protecting autoimmune exocrinopathy.
abstract: BACKGROUND
  Migration of T cells, including regulatory T (Treg) cells, into the secondary lymph organs is critically controlled by chemokines and adhesion molecules. However, the mechanisms by which Treg cells regulate organ-specific autoimmunity via these molecules remain unclear. Although we previously reported autoimmune exocrinopathy resembling Sjögren's syndrome (SS) in the lacrimal and salivary glands from C-C chemokine receptor 7 (CCR7)-deficient mice, it is still unclear whether CCR7 signaling might specifically affect the dynamics and functions of Treg cells in vivo. We therefore investigated the cellular mechanism for suppressive function of Treg cells via CCR7 in autoimmunity using mouse models and human samples.
  
  
  RESULTS
  Patrolling Treg cells were detected in the exocrine organs such as lacrimal and salivary glands from normal mice that tend to be targets for autoimmunity while the Treg cells were almost undetectable in the exocrine glands of CCR7(-/-) mice. In addition, we found the significantly increased retention of CD4(+)CD25(+)Foxp3(+) Treg cells in the lymph nodes of CCR7(-/-) mice with aging. Although Treg cell egress requires sphingosine 1-phosphate (S1P), chemotactic function to S1P of CCR7-/- Treg cells was impaired compared with that of WT Treg cells. Moreover, the in vivo suppression activity was remarkably diminished in CCR7(-/-) Treg cells in the model where Treg cells were co-transferred with CCR7(-/-) CD25(-)CD4(+) T cells into Rag2(-/-) mice. Finally, confocal analysis showed that CCR7(+)Treg cells were detectable in normal salivary glands while the number of CCR7(+)Treg cells was extremely decreased in the tissues from patients with Sjögren's syndrome.
  
  
  CONCLUSIONS
  These results indicate that CCR7 essentially governs the patrolling functions of Treg cells by controlling the traffic to the exocrine organs for protecting autoimmunity. Characterization of this cellular mechanism could have clinical implications by supporting development of new diagnosis or treatments for the organ-specific autoimmune diseases such as Sjögren's syndrome and clarifying how the local immune system regulates autoimmunity.
authors.affiliation: Department of Oral Molecular Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramotocho, Tokushima, Japan.
authors.forename: Naozumi
authors.initials: N
authors.lastname: Ishimaru
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nitta
authors.forename: Rieko
authors.initials: R
authors.lastname: Arakaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Yamada
authors.forename: Martin
authors.initials: M
authors.lastname: Lipp
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takahama
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D002465:Cell Movement; D005088:Exocrine Glands / Q000276:immunology / Q000473:pathology*; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D054400:Receptors, CCR7 / Q000235:genetics / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7