title: Crystallization and preliminary X-ray characterization of the Skp1-Fbg3 complex.
abstract: F-box proteins are the substrate-recognition components of Skp1-Cullin1-F-box protein-Rbx1 (SCF) ubiquitin ligase complexes. Fbs1, an F-box protein, binds specifically to proteins modified with high-mannose oligosaccharides. Fbg3, another F-box protein, has 51% sequence identity to Fbs1. Although the residues that are necessary for binding to oligosaccharides are conserved between Fbs1 and Fbg3, Fbg3 does not bind glycoproteins. Skp1 and Fbg3 were co-expressed in Escherichia coli and their complex was purified to homogeneity and crystallized. Microseeding combined with the sandwiched hanging-drop technique improved the quality of the resulting crystals. The plate-shaped crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.1, b = 76.6, c = 193.9 A and one molecule per asymmetric unit.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Kumanomidou
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mizushima
authors.forename: Atsuo
authors.initials: A
authors.lastname: Suzuki
authors.forename: Fuminori
authors.initials: F
authors.lastname: Tokunaga
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iwai
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Keiji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamane
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D044783:F-Box Proteins / Q000737:chemistry*; D006801:Humans; D044843:SKP Cullin F-Box Protein Ligases / Q000737:chemistry*
chemical_list: D044783:F-Box Proteins; D044843:SKP Cullin F-Box Protein Ligases