Ultrafast pulse-pair control in multiphoton fluorescence laser-scanning microscopy.
In multiphoton fluorescence laser-scanning microscopy, ultrafast laser pulses [i.e., light pulses having pulse width <or=1 ps (1 ps=10(-12) s)] are commonly employed to circumvent the low-multiphoton absorption cross-sections of common fluorophores. Because of the broad overlapping two-photon absorption spectra of fluorophores and the large spectral bandwidth of a short pulse, simultaneous excitation of many fluorophores is common, which justifies a persistent demand for selective excitation of individual fluorophores. We describe the use of pulse-pair excitation with possibilities of controlling molecular fluorescence in laser-scanning microscopy and compare it with coherent control using pulse sequence [De and Goswami, "Coherent control in multiphoton fluorescence imaging," Proc. SPIE 7183, 71832B (2009)].
De
Arijit Kumar
AK
Indian Institute of Technology Kanpur, Department of Chemistry, Kanpur, 208016, India.
Goswami
Debabrata
D
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*
D005456:Fluorescent Dyes