Association between GSTO2 polymorphism and the urinary arsenic profile in copper industry workers.
Two members of the recently identified Omega class glutathione S-transferase enzymes (GSTO1 and GSTO2) have been proposed to play a role in the response to arsenic exposure. Therefore, polymorphisms in these genes could be related with variations in the arsenic excretion profile and, consequently, with the individual response to chronic exposure. Exons and flanking regions of GSTO2 gene have been screened in two different ethnic groups (20 Europeans and 20 Chilean Indians), and the urinary arsenic patterns and the GSTO2 Asn142Asp polymorphism have been investigated in 207 copper mine workers occupationally exposed to arsenic. Three polymorphisms of GSTO2 already described were detected in Europeans and Chilean Indians, although with significant different allele frequencies. The genotyping for the Asn142Asp polymorphism revealed that almost no significant association exists between this change and the arsenic excretion profile. However, 142Asp change seems to be correlated with an increase in DMA excretion after age and total urinary arsenic adjustment (OR=3.61; P=0.05). Altogether, our findings indicate that ethnical differences should be taken into account for correlation studies between GST Omega polymorphisms and arsenic susceptibility, and that the 142Asp allozyme could modulate arsenic biotransformation and thereby arsenic toxicity.
Paiva
Leiliane
L
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Bellaterra, Spain.
Hernández
Alba
A
Martínez
Valeria
V
Creus
Amadeu
A
Quinteros
Domingo
D
Marcos
Ricardo
R
D000328:Adult; D001151:Arsenic / Q000652:urine*; D020261:Arsenic Poisoning / Q000208:ethnology / Q000235:genetics; D002274:Carcinogens, Environmental / Q000032:analysis*; D002677:Chile / Q000208:ethnology; D003300:Copper; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008906:Mining; D016273:Occupational Exposure / Q000032:analysis*; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics
D002274:Carcinogens, Environmental; D003300:Copper; C492569:GSTO2 protein, human; D005982:Glutathione Transferase; D001151:Arsenic