[Analysis of the aminoacyl-tRNA synthetase genes of silkworm (Bombyx mori)].
For further research on number, type, composition and origin of Bombyx mori aminoacyl-tRNA synthetase (BmaaRS) genes, in silico cloning was performed with Bombyx mori genomic and EST databases. There might be two different sets of aaRS nuclear gene in Bombxy nori genome, which encode mitochondrial BmaaRS and cytoplasmic BmaaRS, respectively. Among BmaaRS genes, there were 2 genes encoding mitochondrial BmSerRS, but no genes encoding cytoplasmic BmHisRS and mitochondrial BmGlnRS, BmLysRS, BmGlyRS, and BmThrRS. The functions of these absent genes could be directly replaced by other proteins with similar functions, or might undergo their distinct BmaaRS functions based on the alternative splice of one certain BmaaRS mRNA. Evidence of EST indicated that BmaaRS performed different alternative splicing patterns. The homology comparison and advanced structural analysis of BmaaRS demonstrated the existence of extended domains of BmaaRS. This is because some different BmaaRSs contained similar domain. Moreover, BmaaRSs with similar functions possessed the similar tertiary structure. Phylogenetic analysis revealed that BmaaRS encoded by two various sources of BmaaRS genes. Mitochondrial and cytoplasmic BmaaRS had different origin.
Cao
Guang-Li
GL
Medical College of Soochow University, Suzhou 215123, China. guanglicao@hotmail.com
Xue
Ren-Yu
RY
Zhu
Yue-Xiong
YX
Wei
Yu-Hong
YH
Gong
Cheng-Liang
CL
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny
D019476:Insect Proteins; D000604:Amino Acyl-tRNA Synthetases