title: Platelet, not endothelial, P-selectin expression contributes to generation of immunity in cutaneous contact hypersensitivity.
abstract: Leukocyte extravasation is a prerequisite for host defense and autoimmunity alike. Detailed understanding of the tightly controlled and overlapping sequences of leukocyte extravasation might aid development of novel therapeutic strategies. Leukocyte extravasation is initiated by interaction of selectins with appropriate carbohydrate ligands. Lack of P-selectin expression leads to decreased contact hypersensitivity responses. Yet, it remains unclear if this is due to inhibition of leukocyte extravasation to the skin or due to interference with initial immune activation in lymph nodes. In line with previous data, we here report a decreased contact hypersensitivity response, induced by 2,4,-dinitrofluorobenzene (DNFB), in P-selectin-deficient mice. Eliciting an immune reaction towards DNFB in wild-type mice, followed by adoptive transfer to P-selectin-deficient mice, had no impact on inflammatory response in recipients. This was significantly reduced in wild-type recipient mice adoptively transferred with DNFB immunity generated in P-selectin-deficient mice. To investigate if platelet or endothelial P-selectin was involved, mice solely lacking platelet P-selectin expression generated by bone marrow transplantation were used. Adoptive transfer of immunity from wild-type mice reconstituted with P-selectin-deficient bone marrow led to a decrease of inflammatory response. Comparing this decrease to the one observed using P-selectin-deficient mice, no differences were observed. Our observations indicate that platelet, not endothelial, P-selectin contributes to generation of immunity in DNFB-induced contact hypersensitivity.
authors.affiliation: Department of Dermatology, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany. ralf.ludwig@uk-sh.de
authors.forename: Ralf J
authors.initials: RJ
authors.lastname: Ludwig
authors.forename: Peri
authors.initials: P
authors.lastname: Bergmann
authors.forename: Jurate
authors.initials: J
authors.lastname: Garbaraviciene
authors.forename: Esther
authors.initials: E
authors.lastname: von Stebut
authors.forename: Heinfried H
authors.initials: HH
authors.lastname: Radeke
authors.forename: Jens
authors.initials: J
authors.lastname: Gille
authors.forename: Sandra
authors.initials: S
authors.lastname: Diehl
authors.forename: Katja
authors.initials: K
authors.lastname: Hardt
authors.forename: Reinhard
authors.initials: R
authors.lastname: Henschler
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Josef M
authors.initials: JM
authors.lastname: Pfeilschifter
authors.forename: Wolf-Henning
authors.initials: WH
authors.lastname: Boehncke
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D048430:Cell Shape; D003877:Dermatitis, Contact / Q000150:complications / Q000276:immunology* / Q000473:pathology*; D004139:Dinitrofluorobenzene; D042783:Endothelial Cells / Q000378:metabolism*; D007109:Immunity / Q000276:immunology*; D007249:Inflammation / Q000150:complications / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000378:metabolism*; D012867:Skin / Q000276:immunology / Q000473:pathology*
chemical_list: D019007:P-Selectin; D004139:Dinitrofluorobenzene