Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their anti-invasive activity on HT-29 human colon cancer cells.
We isolated anthocyanins from fruits of Vitis coignetiae Pulliat, characterized the anthocyanin profile, and investigated the anti-invasive effects of the anthocyanins on human colon cancer cells. The anthocyanins inhibited cell invasion in a dose-dependent manner, as measured by Matrigel invasion assays, by suppression of matrix metalloproteinase (MMP)-2 and MMP-9 expression. The anti-invasive activity of the anthocyanins was associated with modulation of constitutive nuclear factor kappaB (NF-kappaB) activation. The activation of NF-kappaB triggered by tumor necrosis factor-alpha was also inhibited by the anthocyanins through suppression IkappaBalpha phosphorylation. AIMs inhibited the expression of NF-kappaB-regulated proteins. In conclusion, this study suggested that the anthocyanins isolated from fruits of V. coignetiae Pulliat should have anti-invasive activities on human colon cancer cells and the activities should be related to the inhibition of NF-kappaB-regulated proteins such as MMP-2 and MMP-9 expression through the inhibition of NF-kappaB activation.
Yun
Jeong Won
JW
Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, South Korea.
Lee
Won Sup
WS
Kim
Min Jeong
MJ
Lu
Jing Nan
JN
Kang
Myung Hee
MH
Kim
Hoon Gu
HG
Kim
Dong Chul
DC
Choi
Eun Ju
EJ
Choi
Jin Young
JY
Kim
Hae Gyeong
HG
Lee
Yun-Kyoung
YK
Ryu
Chung Ho
CH
Kim
Gonsup
G
Choi
Yung Hyun
YH
Park
Ock Jin
OJ
Shin
Sung Chul
SC
D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D015153:Blotting, Western; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D007150:Immunohistochemistry; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D009361:Neoplasm Invasiveness; D021241:Spectrometry, Mass, Electrospray Ionization; D014162:Transfection; D027843:Vitis / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D008156:Luciferases; D051550:I-kappa B Kinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9