Multiple therapeutic effects of adjunctive baicalin therapy in experimental bacterial meningitis.
This study aimed to examine effects of adjunctive baicalin therapy to ampicillin for experimental bacterial meningitis in rabbits. After Escherichia Coli inoculation, mean leukocyte counts, concentrations of protein, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1) and lactate in cerebrospinal fluid (CSF), brain water content and mean arterial and intracranial pressures substantially increased in the meningitis group. Ampicillin alone for 5 h markedly exacerbated the enhanced leukocyte counts and protein concentration, and showed no significant effect on the elevated CSF TNF-alpha, IL-1 and lactate concentration, mean arterial and intracranial pressures, and brain water content. Baicalin (7-D-glucuronic acid-5,6-dihydroxyflavone, C(21)H(18)O(11)) completely counteracted ampicillin-induced exacerbation, and further alleviated the enhanced mean leukocyte counts and protein concentration when combined with ampicillin. Adjunctive baicalin also significantly ameliorated the elevated CSF TNF-alpha, IL-1 and lactate concentration, mean arterial and intracranial pressures, and brain water content. Baicillin, as an adjunctive treatment exerted multiple therapeutic effects in experimental bacterial meningitis.
Tang
Yong-Jun
YJ
Department of Pediatrics, Xiangya Hospital, Central South University, Hunan, China.
Zhou
Fu-Wen
FW
Luo
Zi-Qiang
ZQ
Li
Xin-Zhong
XZ
Yan
Hui-Ming
HM
Wang
Ming-Jie
MJ
Huang
Fu-Rong
FR
Yue
Shao-Jie
SJ
D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D001929:Brain Edema / Q000134:cerebrospinal fluid / Q000188:drug therapy; D002556:Cerebrospinal Fluid Proteins / Q000134:cerebrospinal fluid; D004195:Disease Models, Animal; D004234:Diuretics, Osmotic / Q000494:pharmacology; D004359:Drug Therapy, Combination; D004927:Escherichia coli Infections / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D005419:Flavonoids / Q000494:pharmacology*; D007375:Interleukin-1 / Q000134:cerebrospinal fluid; D007427:Intracranial Pressure / Q000187:drug effects; D019344:Lactic Acid / Q000134:cerebrospinal fluid; D007958:Leukocyte Count; D008353:Mannitol / Q000494:pharmacology; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid; D014867:Water / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D002556:Cerebrospinal Fluid Proteins; D004234:Diuretics, Osmotic; D005419:Flavonoids; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D019344:Lactic Acid; C038044:baicalin; D008353:Mannitol; D000667:Ampicillin