title: Entamoeba invadens, encystation process and enolase.
abstract: The reptilian parasite Entamoeba invadens is accepted as a model for the study of the Entamoeba encystation process. Here we describe the production and characterization of a mAb (B4F2), generated against a component of the E. invadens cyst wall. This mAb specifically recognizes a 48-kDa protein present in cytoplasmic vesicles of cells encysting for 24 h. In mature cysts (96 h), the antigen was detected on the cyst surface. By two-dimensional electrophoresis and mass spectrometry analysis, the B4F2 specific antigen was identified as enolase. Levels of enolase mRNA were increased in encysting cells and the B4F2 mAb was found to inhibit cyst formation. Therefore, these results strongly suggest a new role for enolase in E. invadens encystation, and the B4F2 mAb will be useful tool to study its role in the differentiation process.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508, Zacatenco, 07360 Mexico City, Mexico.
authors.forename: Norma Cristina
authors.initials: NC
authors.lastname: Segovia-Gamboa
authors.forename: Bibiana
authors.initials: B
authors.lastname: Chávez-Munguía
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Medina-Flores
authors.forename: Febe Elena
authors.initials: FE
authors.lastname: Cázares-Raga
authors.forename: Verónica Ivonne
authors.initials: VI
authors.lastname: Hernández-Ramírez
authors.forename: Adolfo
authors.initials: A
authors.lastname: Martínez-Palomo
authors.forename: Patricia
authors.initials: P
authors.lastname: Talamás-Rohana
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004747:Entamoeba / Q000201:enzymology / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053058:Trophozoites / Q000276:immunology / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D010751:Phosphopyruvate Hydratase