title: Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants.
abstract: Our previous studies showed that immunization with recombinant paramyosin from Trichinella spiralis (rTs-Pmy) formulated with Freund's adjuvant significantly reduced larval burden in mice after T. spiralis larval challenge. Since Freund's adjuvant is toxic and not a suitable adjuvant for clinical vaccine trials, we evaluated the ability of the adjuvants Montanide ISA206 and ISA720 to stimulate immune responses during rTs-Pmy immunization and to enhance protective immunity. The results revealed that immunization of BALB/c mice with rTs-Pmy formulated with either ISA206 or ISA720 triggered Th1 and Th2 immune responses similar to those produced by the conventional Freund's adjuvant formulation and also provided a similar level of protection against T. spiralis larval challenge. This indicates that the recombinant Ts-Pmy formulated with Montanide ISA206 or ISA720 may be an effective and safety vaccine strategy for trichinellosis.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, 10 Xitoutiao, You An Men, Beijing 100069, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yang
authors.forename: Junfei
authors.initials: J
authors.lastname: Wei
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.forename: Shijuan
authors.initials: S
authors.lastname: Cui
authors.forename: Jin
authors.initials: J
authors.lastname: Pan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xinping
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009132:Muscles / Q000469:parasitology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014335:Tropomyosin / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014335:Tropomyosin