title: Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell harvest in healthy donors.
abstract: BACKGROUND
  The hematopoietic progenitor cell (HPC) count measured by the Sysmex hematology analyzer can determine the timing for leukapheresis in autologous peripheral blood stem cell (PBSC) harvest. We evaluated whether a HPC count could predict CD34+ cell yield in healthy, unrelated donors after granulocyte-colony-stimulating factor mobilization.
  
  
  METHODS
  A total of 117 healthy donors underwent 161 PBSC leukapheresis procedures in our institution. The HPCs and CD34+ cells were identified by an automated hematology analyzer and flow cytometry, respectively. Using Spearman's rank test, we evaluated the relationships between preharvest HPCs, CD34+ cell counts, and CD34+ cell yields in the apheresis product. A receiver operating characteristic (ROC) curve analysis was used to identify the cutoff value of HPC for adequate mobilization and harvest yield.
  
  
  RESULTS
  The HPC count had a moderate correlation with the preharvest CD34+ cell count (r = 0.502, p < 0.001), and an HPC count of more than 21.3 x 10(6)/L could exclude poor mobilization (<20 x 10(6) CD34+ cells/L) with sensitivity and specificity of 89.2 and 83.3%. However, the relationship between HPC count and CD34+ cell yield was not marked (r = 0.321, p < 0.001). The area under the curve for HPCs was significantly smaller than the preharvest CD34+ cell count on the ROC curve for predicting adequate harvest yield (>10 x 10(6) CD34+ cells/L of processed blood volume, 0.678 vs. 0.850, p = 0.001).
  
  
  CONCLUSIONS
  Although the preapheresis HPC count could predict mobilization in healthy donors before leukapheresis, it may not be a superior index for predicting CD34+ cell yield compared with the preharvest CD34+ cell count.
authors.affiliation: Department of Pediatrics, Hualien Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.
authors.forename: Shang-Hsien
authors.initials: SH
authors.lastname: Yang
authors.forename: Tso-Fu
authors.initials: TF
authors.lastname: Wang
authors.forename: His-Hsiu
authors.initials: HH
authors.lastname: Tsai
authors.forename: Teng-Yi
authors.initials: TY
authors.lastname: Lin
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Wen
authors.forename: Shu-Huey
authors.initials: SH
authors.lastname: Chen
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001782:Blood Donors; D002452:Cell Count; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D012372:ROC Curve
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor