Sodium-translocating NADH:quinone oxidoreductase as a redox-driven ion pump.
The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) is a component of the respiratory chain of various bacteria. This enzyme is an analogous but not homologous counterpart of mitochondrial Complex I. Na+-NQR drives the same chemistry and also uses released energy to translocate ions across the membrane, but it pumps Na+ instead of H+. Most likely the mechanism of sodium pumping is quite different from that of proton pumping (for example, it could not accommodate the Grotthuss mechanism of ion movement); this is why the enzyme structure, subunits and prosthetic groups are completely special. This review summarizes modern knowledge on the structural and catalytic properties of bacterial Na+-translocating NADH:quinone oxidoreductases. The sequence of electron transfer through the enzyme cofactors and thermodynamic properties of those cofactors is discussed. The resolution of the intermediates of the catalytic cycle and localization of sodium-dependent steps are combined in a possible molecular mechanism of sodium transfer by the enzyme.
Verkhovsky
Michael I
MI
Helsinki Bioenergetics Group, Institute of Biotechnology, P.O. Box 65, (Viikinkaari 1), 00014, University of Helsinki, Helsinki, Finland.
Bogachev
Alexander V
AV
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D004579:Electron Transport; D016623:Ion Pumps / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D021122:Protein Subunits; D011808:Quinone Reductases / Q000737:chemistry / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013816:Thermodynamics
D001426:Bacterial Proteins; D016623:Ion Pumps; D021122:Protein Subunits; D012964:Sodium; D011808:Quinone Reductases