Missense mutations in the MEFV gene are associated with fibromyalgia syndrome and correlate with elevated IL-1beta plasma levels.
BACKGROUND
Fibromyalgia syndrome (FMS), a common, chronic, widespread musculoskeletal pain disorder found in 2% of the general population and with a preponderance of 85% in females, has both genetic and environmental contributions. Patients and their parents have high plasma levels of the chemokines MCP-1 and eotaxin, providing evidence for both a genetic and an immunological/inflammatory origin for the syndrome (Zhang et al., 2008, Exp. Biol. Med. 233: 1171-1180).


RESULTS
In a search for a candidate gene affecting inflammatory pathways, among five screened in our patient samples (100 probands with FMS and their parents), we found 10 rare and one common alleles for MEFV, a gene in which various compound heterozygous mutations lead to Familial Mediterranean Fever (FMF). A total of 2.63 megabases of genomic sequence of the MEFV gene were scanned by direct sequencing. The collection of rare missense mutations (all heterozygotes and tested in the aggregate) had a significant elevated frequency of transmission to affecteds (p = 0.0085, one-sided, exact binomial test). Our data provide evidence that rare missense variants of the MEFV gene are, collectively, associated with risk of FMS and are present in a subset of 15% of FMS patients. This subset had, on average, high levels of plasma IL-1beta (p = 0.019) compared to FMS patients without rare variants, unaffected family members with or without rare variants, and unrelated controls of unknown genotype. IL-1beta is a cytokine associated with the function of the MEFV gene and thought to be responsible for its symptoms of fever and muscle aches.


CONCLUSIONS
Since misregulation of IL-1beta expression has been predicted for patients with mutations in the MEFV gene, we conclude that patients heterozygous for rare missense variants of this gene may be predisposed to FMS, possibly triggered by environmental factors.
Feng
Jinong
J
Division of Molecular Genetics, Beckman Research Institute, City of Hope, Duarte, California, United States of America.
Zhang
Zhifang
Z
Li
Wenyan
W
Shen
Xiaoming
X
Song
Wenjia
W
Yang
Chunmei
C
Chang
Frances
F
Longmate
Jeffrey
J
Marek
Claudia
C
St Amand
R Paul
RP
Krontiris
Theodore G
TG
Shively
John E
JE
Sommer
Steve S
SS
D000818:Animals; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005190:Family; D005260:Female; D005356:Fibromyalgia / Q000097:blood* / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D000071198:Pyrin; D035781:Siblings; D013577:Syndrome
D003598:Cytoskeletal Proteins; D053583:Interleukin-1beta; C000604192:MEFV protein, human; D000071198:Pyrin