title: Dip-pen nanolithography on SiOx and tissue-derived substrates: comparison with multiple biological inks.
abstract: There has been extensive interest in the micro and nanoscale manipulation of various substrates in the past few decades. One promising technique is dip-pen nanolithography which has shown the capability to pattern substrates of all forms including, tissue-derived substrates. Patterning of tissue-derived substrates is of particular interest, as it would facilitate studies into controlling cell morphology and cell-substrate interaction. To expand the field into this area both peptides and bioactive collagen-binding peptide-linked biomolecules were patterned to the inner collagenous zone of the Bruch's membrane (BM). Collagen-binding peptide, and extra cellular matrix (ECM) proteins laminin and fibronectin were patterned on the BM and SiO(x). The lithographic protocol was facilitated by Triton X-100 which was used to clean the tissue-derived construct after harvesting. This produced a collagen-exposed BM which was more hydrophilic (contact angle 67 degrees +/-8.49 degrees) surface compared with other cleaning methods but it maintained similar surface roughness (root-mean-square) 80+/-18 nm and collagen exposure. This type of surface can be readily patterned with the chosen inks under lower humidity conditions.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
authors.forename: Marcus A
authors.initials: MA
authors.lastname: Kramer
authors.forename: Heyjin C
authors.initials: HC
authors.lastname: Park
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D016570:Bruch Membrane / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D011485:Protein Binding; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D007797:Laminin; D003094:Collagen