In situ hybridization to identify gut stem cells.
In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.
Gregorieff
Alex
A
Hubrecht Institute, Utrecht, The Netherlands.
Clevers
Hans
H
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004076:Digoxigenin / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D015347:RNA Probes / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D015347:RNA Probes; D004076:Digoxigenin