title: In situ hybridization to identify gut stem cells.
abstract: In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.
authors.affiliation: Hubrecht Institute, Utrecht, The Netherlands.
authors.forename: Alex
authors.initials: A
authors.lastname: Gregorieff
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004076:Digoxigenin / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D015347:RNA Probes / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015347:RNA Probes; D004076:Digoxigenin