[Allergic response to qingkailing injection in BN rats].
This study is to observe allergic response to Qingkailing injection in BN rats and to establish a suitable animal model to evaluate allergic response induced by traditional Chinese medicine. BN rats were sensitized by Qingkailing injection, and guinea pigs were similarly sensitized as the control. The symptoms of allergic response were observed, the levels of histamine in serum and tissues were determined by ELISA assay and pathological changes in lung and trachea were observed with HE staining under light microscope. The total incidence of allergic response in BN rats was 52.78%, which was higher than that in guinea pig groups (16.67%). The total degree of allergic response in BN rats was higher than that in guinea pigs. Compared with control groups, the level of histamine in serum, lung and trachea tissues of BN rats and guinea pigs increased significantly. The release rate of histamine in BN rats was higher than that in guinea pigs. The rate and degree of pathological changes in lung and trachea tissues of BN rats were higher than that in guinea pigs. Compared with guinea pig, BN rat is probably a suitable animal model in evaluating allergic response to injection of traditional Chinese medicine.
Guo
Shan-Shan
SS
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Yi-Zhong
YZ
Zhang
Yi
Y
Li
De-Feng
DF
Shi
Yu-Jing
YJ
Gao
Ying-Jie
YJ
Cui
Xiao-Lan
XL
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000009:adverse effects*; D006168:Guinea Pigs; D006632:Histamine / Q000097:blood; D006969:Hypersensitivity, Immediate / Q000139:chemically induced*; D007267:Injections; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN
D004365:Drugs, Chinese Herbal; C504928:Qingkailing; D006632:Histamine