title: In vivo development of vitrified rabbit embryos: effects on prenatal survival and placental development.
abstract: The aim of this work is to study the effect of the vitrification procedure on prenatal survival and on placental development at the end of gestation in rabbits (Oryctolagus cuniculus). One hundred eighty-one females were slaughtered at 72 h of gestation. Morphologically normal embryos recovered at 72 h of gestation were kept at room temperature until transfer or vitrification. Vitrified embryos (320 embryos) were transferred into a total of 24 does and fresh embryos (712 embryos) were transferred into a total of 43 does. Females were induced to ovulate 72 h before transfer when fresh embryos were transferred and 60 to 63 h before transfer when vitrified embryos were transferred. Each recipient doe received eight embryos into the left oviduct and eight embryos into the right oviduct. The number of implanted embryos was estimated by laparoscopy as number of implantation sites at Day 14 of gestation. Recipient females were slaughtered by stunning and exsanguination 25 d after the transfer, and fetuses were classified according to their status. Live fetuses and fetal and maternal placenta were weighed Pregnancy rate was defined as the total number of females having at least one live fetus at Day 28 of gestation divided by the total number of females. Prenatal survival was estimated as live fetuses at Day 28 of gestation divided by the number of transferred embryos. The pregnancy rate after transfer of vitrified embryos (92%) was similar to that achieved with fresh embryos (86%), but prenatal survival was lower for vitrified than for fresh embryos (53% vs. 34%). We did not find differences in embryo survival from 72 h to implantation. Transfer of vitrified embryos reduced fetal survival from implantation to Day 28 (57% vs. 82%). Differences in the number of live fetuses at Day 28 of gestation were mainly due to the higher fetal mortality observed soon after implantation in pregnancies resulting from the transfer of vitrified embryos. A higher percentage of decidual reactions and atrophic maternal placentas (27.5% vs. 8.3%) and also of atrophic fetal and maternal placentas (12.1% vs. 5.3%) were observed after transfer of vitrified embryos. Both treatments showed similar percentage of dead fetuses (3.3% vs. 4%). Maternal placenta of the fetuses from fresh embryos was 15% heavier than maternal placenta of fetuses from vitrified embryos.
authors.affiliation: Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, Valencia, Spain. mmoce@uch.ceu.es
authors.forename: M L
authors.initials: ML
authors.lastname: Mocé
authors.forename: A
authors.initials: A
authors.lastname: Blasco
authors.forename: M A
authors.initials: MA
authors.lastname: Santacreu
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology*; D004973:Estrus Synchronization; D005260:Female; D005328:Fetal Viability / Q000502:physiology*; D020567:Fetal Weight / Q000502:physiology; D008098:Litter Size; D008297:Male; D010929:Placentation; D011247:Pregnancy; D018873:Pregnancy Rate; D011817:Rabbits