title: The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus.
abstract: The Epstein-Barr virus efficiently infects human B cells. The EBV genome is maintained extrachromosomally and replicates synchronously with the host's chromosomes. The latent origin of replication (oriP) guarantees plasmid stability by mediating two basic functions: replication and segregation of the viral genome. While the segregation process of EBV genomes is well understood, little is known about its chromatin association and nuclear distribution during interphase. Here, we analyzed the nuclear localization of EBV genomes and the role of functional oriP domains FR and DS for basic functions such as the transformation of primary cells, their role in targeting EBV genomes to distinct nuclear regions, and their association with epigenetic domains. Fluorescence in situ hybridization visualized the localization of extrachromosomal EBV genomes in the regions adjacent to chromatin-dense territories called the perichromatin. Further, immunofluorescence experiments demonstrated a preference of the viral genome for histone 3 lysine 4-trimethylated (H3K4me3) and histone 3 lysine 9-acetylated (H3K9ac) nuclear regions. To determine the role of FR and DS for establishment and subnuclear localization of EBV genomes, we transformed primary human B lymphocytes with recombinant mini-EBV genomes containing different oriP mutants. The loss of DS results in a slightly increased association in H3K27me3 domains. This study demonstrates that EBV genomes or oriP-based extrachromosomal vector systems are integrated into the higher order nuclear organization. We found that viral genomes are not randomly distributed in the nucleus. FR but not DS is crucial for the localization of EBV in perichromatic regions that are enriched for H3K4me3 and H3K9ac, which are hallmarks of transcriptionally active regions.
authors.affiliation: Department of Gene Vectors, HelmholtzZentrum Muenchen, Munich, Germany.
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Deutsch
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ott
authors.forename: Peer
authors.initials: P
authors.lastname: Papior
authors.forename: Aloys
authors.initials: A
authors.lastname: Schepers
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D018741:Replication Origin; D014779:Virus Replication / Q000235:genetics*
chemical_list: D002843:Chromatin; D019309:Epstein-Barr Virus Nuclear Antigens; D006657:Histones; C417029:EBV-encoded nuclear antigen 1