title: [Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen].
abstract: OBJECTIVE
  To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).
  
  
  METHODS
  The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.
  
  
  RESULTS
  The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).
  
  
  CONCLUSIONS
  Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.
authors.affiliation: Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. qiuchengfeng0721@yahoo.com.cn
authors.forename: Cheng-feng
authors.initials: CF
authors.lastname: Qiu
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: Lei
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Wu
authors.forename: Chuan-lin
authors.initials: CL
authors.lastname: Yu
authors.forename: Zheng-guang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Na-na
authors.initials: NN
authors.lastname: Chen
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000097:blood; D056724:Immunity, Humoral; D007114:Immunization; D008297:Male; D051379:Mice / Q000276:immunology*; D023421:Models, Animal; D011817:Rabbits
chemical_list: D006460:Hemolysin Proteins