Switchgrass leaching requirements for solid-state fermentation by Acidothermus cellulolyticus.
Growth of Acidothermus cellulolyticus in solid-state fermentation and its required growth conditions were investigated in this study. Extraction of switchgrass was required for growth. Under the experimental conditions, extraction ratio had the most significant effect on the growth of A. cellulolyticus. Heat treatment (in the form of autoclaving) of switchgrass did not have a significant effect on the growth rate; however, longer heat treatment times had a negative effect on the total growth. Moisture content adjustment had no effect on the release of inhibitors into extracts. Our results showed that leaching at a minimum 40:1 (gram water: gram dry biomass) removed inhibitory compound(s) from switchgrass. Upon extraction A. cellulolyticus colonized switchgrass in solid fermentation without exogenous addition of carbon and nitrogen sources. It is the first demonstration of growth of A. cellulolyticus in solid fermentation.
VanderGheynst
Jean S
JS
Dept. of Biological and Agricultural Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. jsvander@ucdavis.edu
Rezaei
Farzaneh
F
Dooley
Todd M
TM
Berry
Alison M
AM
D000192:Actinomycetales / Q000254:growth & development* / Q000378:metabolism*; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D016018:Least-Squares Analysis; D008897:Panicum / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D013227:Steam
D003470:Culture Media; D010936:Plant Extracts; D013227:Steam; D002245:Carbon Dioxide; D002482:Cellulose