title: CD4(+)CD25(+/hi)CD127(lo) phenotype does not accurately identify regulatory T cells in all populations of HIV-infected persons.
abstract: The most commonly used regulatory T cell (Treg) phenotypes, CD4(+)CD25(hi)CD127(lo) and CD4(+)CD25(hi)FoxP3(+), were simultaneously used to determine the Treg frequency in 2 different groups of human immunodeficiency virus (HIV)-infected persons, one viremic and other aviremic. As expected, a strong correlation between both Treg phenotypes was observed in the aviremic group (r = 0.913; P < .001), but surprisingly, this correlation was completely absent in the viremic group (r = 0.143; P = .572). Data on T cell activation levels of both HIV groups suggest that the CD4(+)CD25(hi)CD127(lo) phenotype could just be mirroring the elevated numbers of activated non-regulatory T cells in the viremic HIV group.
authors.affiliation: Immunovirology Laboratory, Infectious Diseases Service, Institute of Biomedicine of Seville, Virgen del Rocío Universitary Hospital, Seville, Spain.
authors.forename: Maria Del Mar
authors.initials: Mdel M
authors.lastname: Del Pozo-Balado
authors.forename: Manuel
authors.initials: M
authors.lastname: Leal
authors.forename: Gema
authors.initials: G
authors.lastname: Méndez-Lagares
authors.forename: Yolanda María
authors.initials: YM
authors.lastname: Pacheco
mesh_terms: D000328:Adult; D015704:CD4 Antigens; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000378:metabolism*; D014766:Viremia
chemical_list: D015704:CD4 Antigens; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit