Examining the contents of isolated Xenopus germinal vesicles.
One can manually isolate the giant oocyte nucleus or germinal vesicle (GV) of Xenopus from a living oocyte with nothing more complicated than jewelers' forceps and a dissecting microscope. Similarly, one can remove the nuclear envelope by hand and allow the lampbrush chromosomes and other nuclear organelles to spread on a microscope slide. After centrifugation, the nuclear contents adhere tightly to the slide, where they can be subjected to immunostaining or fluorescent in situ hybridization for visualization by conventional or confocal microscopy. Preparations of isolated GV contents reveal details of nuclear structure that are almost impossible to attain by more conventional techniques.
Gall
Joseph G
JG
Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA. gall@ciwemb.edu
Wu
Zheng'an
Z
D000465:Algorithms; D000818:Animals; D054239:Blastodisc / Q000166:cytology*; D002448:Cell Adhesion; D002466:Cell Nucleolus / Q000378:metabolism; D002875:Chromosomes / Q000648:ultrastructure*; D003584:Cytological Techniques; D005898:Glass; D006863:Hydrogen-Ion Concentration; D017403:In Situ Hybridization; D008853:Microscopy / Q000379:methods; D009685:Nuclear Envelope / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D014562:Caudata / Q000378:metabolism; D014981:Xenopus / Q000378:metabolism*