Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing.
OBJECTIVE
To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility.


METHODS
The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA).


RESULTS
We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells.


CONCLUSIONS
The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.
Liu
Tie-lian
TL
Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
Miao
Jing-cheng
JC
Sheng
Wei-hua
WH
Xie
Yu-feng
YF
Huang
Quan
Q
Shan
Yun-bo
YB
Yang
Ji-cheng
JC
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D002448:Cell Adhesion; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005349:Fibroins / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D008954:Models, Biological; D047011:Silk / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
D001672:Biocompatible Materials; D047011:Silk; D013778:Tetrazolium Salts; D013844:Thiazoles; D042461:Vascular Endothelial Growth Factor A; D005349:Fibroins; C022616:thiazolyl blue