title: Comparison of bone marrow stromal cells derived from stroke and normal rats for stroke treatment.
abstract: OBJECTIVE
  We compared the effect of treatment of stroke with bone marrow stromal cells from stroke rats (Isch-BMSC) and normal rats (Nor-BMSC) on functional outcome.
  
  
  METHODS
  Isch-BMSCs and Nor-BMSCs were intravenously injected into rats 24 hours after middle cerebral artery occlusion. To test the mechanism of Isch-BMSC-enhanced neurorestoration, Isch-BMSC and Nor-BMSC cultures were used.
  
  
  RESULTS
  Isch-BMSC significantly promoted functional outcome and enhanced angiogenesis, arterial density, and axonal regeneration compared with Nor-BMSC treatment animals. Isch-BMSCs exhibited increased Angiopoietin-1, Tie2, basic fibroblast growth factor, glial cell-derived neurotrophic factor, vascular endothelial growth factor, and Flk1 gene expression compared with Nor-BMSC. Using transwell coculture of BMSCs with brain-derived endothelial cells, Isch-BMSCs increased phosphorylated-Tie2 activity in brain-derived endothelial cells and enhanced brain-derived endothelial cells capillary tube formation compared with Nor-BMSCs. Inhibition of Tie2 gene expression in brain-derived endothelial cells using siRNA significantly attenuated BMSC-induced capillary tube formation.
  
  
  CONCLUSIONS
  These data suggest that Isch-BMSCs are superior to Nor-BMSCs for the neurorestorative treatment of stroke, which may be mediated by the enhanced trophic factor and angiogenic characteristics of Isch-BMSCs.
authors.affiliation: Department of Neurology, Henry Ford Health Sciences Center, Detroit, Mich 48202, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Zacharek
authors.forename: Amjad
authors.initials: A
authors.lastname: Shehadah
authors.forename: Jieli
authors.initials: J
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Cui
authors.forename: Cynthia
authors.initials: C
authors.lastname: Roberts
authors.forename: Mei
authors.initials: M
authors.lastname: Lu
authors.forename: Michael
authors.initials: M
authors.lastname: Chopp
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000473:pathology* / Q000601:surgery*; D017154:Stromal Cells / Q000637:transplantation; D016896:Treatment Outcome