ESR micro-imaging of LiNc-BuO crystals in PDMS: spatial and spectral grain distribution.
Microcrystals of lithium octa-n-butoxynaphthalocyanine (LiNc-BuO) in a bio-compatible and oxygen-permeable polymer matrix of poly-dimethyl-siloxane (PDMS) can be used for repetitive non-invasive imaging of oxygen in live specimens by means of mm-scale electron spin resonance (ESR) imaging. This probe denoted as "oxychip" was characterized by high-resolution mum-scale ESR microcopy to reveal the fine details of its spatial and spectral properties. The ESR micro-images of a typical oxychip device showed that while the spatial distribution of the microcrystals in the polymer is fairly homogenous (as revealed by optical microscopy), the ESR signal originates only from a very few dominant crystals. Furthermore, spectral-spatial analysis in a microcrystal and a sub-microcrystal spatial resolution reveals that each crystal has a slightly different g-factor and also exhibits variations in linewidth, possibly due to the slightly different individual crystallization process.
Blank
Aharon
A
Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa 32000, Israel. ab359@tx.technion.ac.il
Halevy
Revital
R
Shklyar
Michael
M
Shtirberg
Lazar
L
Kuppusamy
Periannan
P
D000465:Algorithms; D003461:Crystallography / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008665:Metalloporphyrins / Q000737:chemistry*; D008838:Microcomputers; D012984:Software
D004129:Dimethylpolysiloxanes; D008665:Metalloporphyrins; C481390:lithium 5,9,14,18,23,27,32,36-octa-n-butoxy-2,3-naphthocyanine; C013830:baysilon