Splenic autotransplantation restores IL-17 production and antibody response to Streptococcus pneumoniae in splenectomized mice.
The high incidence of overwhelming postsplenectomy infection caused by Streptococcus pneumoniae can be reduced by splenic autotransplantation. In this study the effect of splenectomy and splenic autotransplantation on the immune response to S. pneumoniae infection was investigated. Balb/c mice were divided into three groups: splenectomized (SP), splenectomized and autotransplanted (AT), and sham operated control (CT). Five days post-infection the serum antibody levels were measured and the number of S. pneumoniae CFU, neutrophil accumulation and IL-17 production in the liver and lungs were investigated. SP mice showed greater number of bacteria in both organs and lower serum levels of S. pneumoniae-specific IgM, IgG1 and IgG2a antibodies. IL-17 production and neutrophil recruitment to the liver and lungs were lower in SP mice, in comparison with both the CT and the AT groups. Levels of S. pneumoniae-specific IgM, CFU counts, neutrophil accumulation and IL-17 production did not differ significantly between the CT and AT groups. These results suggest that splenic autotransplantation restores the capacity of splenectomized mice to fight S. pneumoniae infection.
Fernandes
B F
BF
Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Minas Gerais, Brazil.
Rezende
A B
AB
Alves
C C S
CC
Teixeira
F M
FM
Farias
R E
RE
Ferreira
A P
AP
Teixeira
H C
HC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002465:Cell Movement; D015169:Colony Count, Microbial; D007109:Immunity; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000473:pathology; D011008:Pneumococcal Infections / Q000097:blood / Q000276:immunology* / Q000601:surgery / Q000628:therapy; D013156:Splenectomy / Q000009:adverse effects; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D014182:Transplantation, Autologous; D014774:Virulence
D000907:Antibodies, Bacterial; D020381:Interleukin-17