A high recovery microsampling device based on a microdialysis probe for peptide sampling.
A high recovery microsampling probe based on microdialysis was devised. The new probe showed a high recovery (100%) of peptides in vitro at different perfusion flow rates (0.1-1.0 microl/min). At a high flow rate, 1.0 microl/min, a 10-fold increased in recovery of peptides compared to the conventional microdialysis probe was achieved. A probe made of a low molecular weight cutoff membrane is suitable for filtering off proteins. The new probe can be a useful tool for high recovery of peptides from living tissues.
Roy
Michael C
MC
Okinawa Institute of Science and Technology (OIST), Suzaki 12-2, Uruma, Okinawa 904-2234, Japan. mcroy@oist.jp
Ikimura
Kazuko
K
Nishino
Hirohito
H
Naito
Takayuki
T
D000180:Acrylic Resins / Q000737:chemistry; D004743:Enkephalin, Leucine / Q000737:chemistry / Q000302:isolation & purification; D017551:Microdialysis / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D010477:Perfusion; D011108:Polymers / Q000737:chemistry; D013450:Sulfones / Q000737:chemistry; D014667:Vasopressins / Q000737:chemistry / Q000302:isolation & purification
D000180:Acrylic Resins; D010455:Peptides; D011108:Polymers; D013450:Sulfones; D014667:Vasopressins; C010504:polyacrylonitrile; C017662:polysulfone P 1700; D004743:Enkephalin, Leucine