[Screening of proteins binding to FXR1P using yeast two-hybrid technique].
OBJECTIVE
To screen the proteins interacting with FXR1P for functional investigation of FXR1P.


METHODS
The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.


RESULTS
The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.


CONCLUSIONS
These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.
Su
Jiao
J
Department of Biochemistry, Changzhi Medical College, Changzhi 046000, China. sujiao1028@126.com
He
Shu-ya
SY
Li
Bin-yuan
BY
Ma
Yun
Y
Yu
Chang-shun
CS
D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000235:genetics / Q000378:metabolism; D005293:Ferritins / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D001324:Autoantigens; C096916:FXR1 protein, human; C098772:GOLGA4 protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D005293:Ferritins; C454461:FTH1 protein, human; D010088:Oxidoreductases; D004960:Estradiol Dehydrogenases; C513331:HSD17B1 protein, human