title: Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells.
abstract: Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Ialpha and Ibeta and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Ialpha and Ibeta led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-gamma, tumor necrosis factor-alpha, granulocyte-colony stimulating factor or transforming growth factor-beta1. The elevated IL-6 expression seen in REG Ialpha transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology.
authors.affiliation: Department of Biochemistry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Usami
authors.forename: Satoru
authors.initials: S
authors.lastname: Motoyama
authors.forename: Souichi
authors.initials: S
authors.lastname: Koyota
authors.forename: Jingshu
authors.initials: J
authors.lastname: Wang
authors.forename: Kaori
authors.initials: K
authors.lastname: Hayashi-Shibuya
authors.forename: Kiyotomi
authors.initials: K
authors.lastname: Maruyama
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Hajime
authors.initials: H
authors.lastname: Saito
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Minamiya
authors.forename: Shin
authors.initials: S
authors.lastname: Takasawa
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Ogawa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D051847:Lithostathine / Q000235:genetics / Q000378:metabolism*; D018307:Neoplasms, Squamous Cell / Q000378:metabolism*; D014162:Transfection
chemical_list: D015850:Interleukin-6; D051847:Lithostathine; C497617:REG1A protein, human; C497618:REG1B protein, human