title: The performance of photooxidatively crosslinked acellular bovine jugular vein conduits in the reconstruction of connections between pulmonary arteries and right ventricles.
abstract: In this study, valved photooxidatively crosslinked acellular bovine jugular vein conduits (BJVCs) were implanted in young dogs to reconstruct the connections of pulmonary arteries and right ventricles, with acellular conduits used as controls. All acellular conduits had moderate to severe valvular dysfunction and were explanted at 1-month implantation (n = 5). Histological examination showed inflammatory cell infiltration and intimal hyperplasia in the walls, and severe inflammatory cell infiltration and thrombosis in the valves. The photooxidatively crosslinked acellular conduits were retrieved at 1-month (n = 5) and 6-month (n = 5) implantations respectively. These conduits had excellent valvular function at retrieval. Their walls and valves were still soft and smooth without calcification and hemangioma. Endothelialization in valves and luminal walls was unsatisfied at 1-month retrieval, and was improved at 6-month retrieval. Host cells infiltrated and migrated from outer layer to the middle layer, with tissue remolding and regeneration found in these recellular regions. Histological examination and tissue content assay demonstrated that degeneration and regeneration of collagens and glycosaminoglycans were comparable, but elastic fibers gradually degraded. Photooxidatively crosslinked acellular BJVCs resist calcification and thrombosis and have regeneration patterns, with excellent hemodynamic performance.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, PR China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Lü
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhong-Shi
authors.initials: ZS
authors.lastname: Wu
authors.forename: Tie-Hui
authors.initials: TH
authors.lastname: Hu
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Ye-Rong
authors.initials: YR
authors.lastname: Hu
mesh_terms: D000818:Animals; D019917:Blood Vessel Prosthesis Implantation; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004285:Dogs; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006351:Heart Valves / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000187:drug effects / Q000601:surgery*; D007150:Immunohistochemistry; D007601:Jugular Veins / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000637:transplantation*; D008027:Light; D008168:Lung / Q000187:drug effects / Q000473:pathology; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D011651:Pulmonary Artery / Q000000981:diagnostic imaging / Q000187:drug effects / Q000601:surgery*; D019651:Plastic Surgery Procedures / Q000379:methods*; D054457:Tissue Scaffolds; D014463:Ultrasonography
chemical_list: D003432:Cross-Linking Reagents