title: Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells.
abstract: Adult mammalian neurogenesis occurs in the hippocampus and the olfactory bulb, whereas neocortical adult neurogenesis remains controversial. Several occurrences of neocortical adult neurogenesis in injured neocortex were recently reported, suggesting that neural stem cells (NSCs) or neuronal progenitor cells (NPCs) that can be activated by injury are maintained in the adult brain. However, it is not clear whether or where neocortical NSCs/NPCs exist in the brain. We found NPCs in the neocortical layer 1 of adult rats and observed that their proliferation was highly activated by global forebrain ischemia. Using retrovirus-mediated labeling of layer 1 proliferating cells with membrane-targeted green fluorescent protein, we found that the newly generated neurons were GABAergic and that the neurons were functionally integrated into the neuronal circuitry. Our results suggest that layer 1 NPCs are a source of adult neurogenesis under ischemic conditions.
authors.affiliation: Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan. ohira@fujita-hu.ac.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Ohira
authors.forename: Takahiro
authors.initials: T
authors.lastname: Furuta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hioki
authors.forename: Kouichi C
authors.initials: KC
authors.lastname: Nakamura
authors.forename: Eriko
authors.initials: E
authors.lastname: Kuramoto
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Nobuo
authors.initials: N
authors.lastname: Funatsu
authors.forename: Keiko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Takao
authors.initials: T
authors.lastname: Oishi
authors.forename: Motoharu
authors.initials: M
authors.lastname: Hayashi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Shun
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D049109:Cell Proliferation; D008297:Male; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016548:Prosencephalon / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000503:physiopathology*; D055153:Stem Cell Niche / Q000503:physiopathology; D013569:Synapses / Q000502:physiology; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005680:gamma-Aminobutyric Acid