title: Serodiagnostic efficacy of Mycobacterium tuberculosis 30/32-kDa mycolyl transferase complex, ESAT-6, and CFP-10 in patients with active tuberculosis.
abstract: Elimination of tuberculosis (TB) largely depends upon definitive rapid diagnosis and treatment. Widely used diagnostic tests do not qualify for use in a developing country due to lack of either desired accuracy or their cost. In the present study an enzyme-linked immunosorbent assay was used to evaluate the diagnostic potential of an immuno-dominant 30/32-kDa mycolyl transferase complex (Ag85 complex) and Mycobacterium tuberculosis-specific proteins (ESAT-6 and CFP-10) of the RD1 region. Higher sensitivity (84.1%) with Ag85 complex was observed compared with ESAT-6 (64.9%) and CFP-10 (66%), with almost similar specificity (Ag85: 85.2%, ESAT-6: 88.9%, CFP-10: 85.2%), whereas the individual components of Ag85 complex, i.e. Ag85A, Ag85B, and Ag85C, showed sensitivities of 44.6, 34, and 80.9% and specificities of 55.6, 74.1, and 40.7% respectively. A cocktail of Ag85 complex, ESAT-6, CFP-10, Ag85A, Ag85B, and Ag85C antigens also could not help in increasing either sensitivity (51.1%) or specificity (85.2%). Furthermore, immunoblot analysis using clinical isolates as well as a standard strain (H37Rv) of M. tuberculosis also showed strong reactivity of sera from TB patients to Ag85 complex and, to a lesser extent, also to ESAT-6. To conclude, use of Ag85 complex along with ESAT-6 and CFP-10 seems to be promising in minimizing the heterogeneous sero-responses of adult TB cases.
authors.affiliation: National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Taj Ganj, Agra, India.
authors.forename: Gavish
authors.initials: G
authors.lastname: Kumar
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Dagur
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: Hari
authors.initials: H
authors.lastname: Shankar
authors.forename: Virendra S
authors.initials: VS
authors.lastname: Yadav
authors.forename: Vishwa M
authors.initials: VM
authors.lastname: Katoch
authors.forename: Bharat
authors.initials: B
authors.lastname: Bajaj
authors.forename: Rajesh
authors.initials: R
authors.lastname: Gupta
authors.forename: Utpal
authors.initials: U
authors.lastname: Sengupta
authors.forename: Beenu
authors.initials: B
authors.lastname: Joshi
mesh_terms: D000217:Acyltransferases / Q000032:analysis* / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis