title: Reduced Ca2+ transport across sarcolemma but enhanced spontaneous activity in cardiomyocytes isolated from left atrium-pulmonary veins tissue of myopathic hamster.
abstract: BACKGROUND
  Several lines of evidence point to a particularly important role of the left atrium (LA) in initiating and maintaining atrial fibrillation (AF). This role may be related to the location of pulmonary veins (PVs) in the LA. The aim of the present study was to investigate the action potential (AP) and ionic currents in LA-PV cardiomyocytes isolated from Bio14.6 myopathic Syrian hamsters (36-57 week-old) versus age-matched F1B healthy control hamsters.
  
  
  RESULTS
  Whole-cell patch-clamp techniques were used to record AP in current-clamp mode and ionic currents in voltage-clamp mode. The results obtained show that in both healthy and myopathic LA-PV tissue spontaneously discharging cardiomyocytes can be found, but they are more numerous in myopathic (9/29) than in healthy hamsters (4/42, p < 0.05 by chi2 analysis). Myopathic myocytes have shorter AP duration (APD) with smaller ICa,L and INCX than the healthy control. The currents ITO, IK, IK1 and ICa,T are not significantly different in myopathic versus healthy cells.
  
  
  CONCLUSIONS
  Our results indicate that in myopathic Syrian hamsters LA-PV cardiomyocytes are more prone to automatic rhythms. Also, they show altered electrophysiologic properties, which may be due to abnormal Ca2+ channels and may account for contractile dysfunction.
authors.affiliation: Institute of Physiology, National Defense Medical Center, Taipei, Taiwan, ROC. ryanoine@yahoo.com.tw
authors.forename: Yue-Xia
authors.initials: YX
authors.lastname: Loh
authors.forename: Kuo-Ho
authors.initials: KH
authors.lastname: Wu
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Chih-Hsiung
authors.initials: CH
authors.lastname: Hsu
authors.forename: Jeng
authors.initials: J
authors.lastname: Wei
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Lin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism; D006224:Cricetinae; D006325:Heart Atria / Q000166:cytology*; D006333:Heart Failure / Q000378:metabolism / Q000503:physiopathology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011667:Pulmonary Veins / Q000166:cytology*; D012508:Sarcolemma / Q000378:metabolism*
chemical_list: D020746:Calcium Channels, L-Type; D002118:Calcium