Correspondence between retinal reflectometry and a flicker-based technique in the measurement of macular pigment spatial profiles.
A comparison of macular pigment optical density (MPOD) spatial profiles determined by an optical and a psychophysical technique is presented. We measured the right eyes of 19 healthy individuals, using fundus reflectometry at 0, 1, 2, 4, 6, and 8 deg eccentricity; and heterochromatic flicker photometry (HFP) at 0, 0.5, 1, 2, 3, 4, 5, 6, and 7 deg, and a reference point at 8 deg eccentricity. We found a strong correlation between the two techniques. However, the absolute estimates obtained by fundus reflectometry data were higher than by HFP. These differences could partly be explained by the fact that at 8 deg eccentricity the MPOD is not zero, as assumed in HFP. Furthermore, when performing HFP for eccentricities of <1 deg, we had to assume that subjects set flicker thresholds at 0.4 deg horizontal translation when using a 1-deg stimulus. MPOD profiles are very similar for both techniques if, on average, 0.05 DU is added to the HFP data at all eccentricities. An additional correction factor, dependent on the steepness of the MPOD spatial distribution, is required for 0 deg.
van der Veen
Rob L P
RL
University Eye Clinic Maastricht, P.O. Box 5800, NL-6202 AZ Maastricht, The Netherlands.
Berendschot
Tos T J M
TT
Makridaki
Maria
M
Hendrikse
Fred
F
Carden
David
D
Murray
Ian J
IJ
D000328:Adult; D005260:Female; D005654:Fundus Oculi; D006801:Humans; D008297:Male; D010783:Photometry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012160:Retina / Q000033:anatomy & histology*