title: Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing.
abstract: OBJECTIVE
  To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility.
  
  
  METHODS
  The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA).
  
  
  RESULTS
  We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells.
  
  
  CONCLUSIONS
  The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.
authors.affiliation: Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
authors.forename: Tie-lian
authors.initials: TL
authors.lastname: Liu
authors.forename: Jing-cheng
authors.initials: JC
authors.lastname: Miao
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Sheng
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Xie
authors.forename: Quan
authors.initials: Q
authors.lastname: Huang
authors.forename: Yun-bo
authors.initials: YB
authors.lastname: Shan
authors.forename: Ji-cheng
authors.initials: JC
authors.lastname: Yang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D002448:Cell Adhesion; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005349:Fibroins / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D008954:Models, Biological; D047011:Silk / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D013778:Tetrazolium Salts; D013844:Thiazoles; D042461:Vascular Endothelial Growth Factor A; D005349:Fibroins; C022616:thiazolyl blue