Identification of cows treated with recombinant bovine somatotropin.
A method for the specific detection and quantification of recombinant bovine somatotropin (rbST) in bovine blood has been validated according to criteria described in the EU Commission Decision 2002/657/EC. The method is based on a thorough purification procedure followed with the detection by LC-ESI-MS/MS of the tryptic N-terminal peptide specific of the rbST. The recombinant equine somatotropin (reST) is used as internal standard. Performance of the method was assessed based on specificity, linearity, trueness and repeatability. Decision limit (CCalpha) and detection capability (CCbeta) were found to be 2.5 ng mL(-1) and 6.8 ng mL(-1), respectively. This method was subsequently applied to the analysis of serum and plasma collected from two different animals treated with 500 mg of rbST. No significant variations were observed when analyzing either serum or plasma, but an important difference between animals was encountered. In all cases, recombinant bovine somatotropin was still detected two weeks after administration.
Le Breton
Marie-Héléne
MH
Nestle Research Center, Nestec Ltd PO Box 44, CH-1000 Lausanne 26, Switzerland. marie-helene.le-breton@rdls.nestle.com
Rochereau-Roulet
Sandrine
S
Chéreau
Sylvain
S
Pinel
Gaud
G
Delatour
Thierry
T
Le Bizec
Bruno
B
D000818:Animals; D002417:Cattle / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013006:Growth Hormone / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*; D007700:Kinetics; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013006:Growth Hormone