Generation and identification of pluripotent stem cells from human embryonic fibroblast cells by 4 defined factors.
OBJECTIVE
To generate and identify the induced pluripotent stem cells (iPSCs) from human embryonic fibroblast cells (hEFs) by introducing 4 defined factors.


METHODS
We introduced 4 factors (Oct4, Sox2, Nanog,and Lin28) into hEFs by lentivirus infection. The iPSCs generated from this method were analyzed in many aspects, including surface antigens, gene expression, and telomerase activity, differentiation ability in vivo and in vitro and the patterns of short tandem repeat (STR).


RESULTS
The human iPSCs generated from this study were positive for alkaline phosphatase (AKP) staining, expressed hESCs-specific surface antigens, and exhibited high telomerase activity. They also possessed the ability to differentiate into 3-germ layers both in vivo and in vitro. STR analysis indicated that the human iPSCs were derived from the donor material.


CONCLUSIONS
The iPSCs lines can be obtained from human embryonic fibroblast by introducing 4 factors. Such human iPSCs should be useful in the study of epigenetic reprogramming and pluripotency maintenance.
Du
Lili
L
Institute of Reproductive & Stem Cell Engineering, Central South University, Changsha 410078, China.
Lin
Ge
G
Lu
Guangxiu
G
D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D018398:Homeodomain Proteins; C000715187:Lin28A protein, human; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016601:RNA-Binding Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors