Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase.
The role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the lead (Pb)-induced cellular response was investigated in the cultured endothelial cells. Pb caused progressive cellular death in endothelial cells, which occurred in a concentration- and time-dependent manner. However, Ref-1 overexpression with AdRef-1 significantly inhibited Pb-induced cell death in the endothelial cells. Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Pb exposure induced the downregulation of catalase, it was inhibited by the Ref-1 overexpression in the endothelial cells. Taken together, our data suggests that the overexpression of Ref-1 inhibited Pb-induced cell death via the upregulation of catalase in the cultured endothelial cells.
Lee
Kwon Ho
KH
Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Korea.
Lee
Sang Ki
SK
Kim
Hyo Shin
HS
Cho
Eun Jung
EJ
Joo
Hee Kyoung
HK
Lee
Eun Ji
EJ
Lee
Ji Young
JY
Park
Myoung Soo
MS
Chang
Seok Jong
SJ
Cho
Chung-Hyun
CH
Park
Jin Bong
JB
Jeon
Byeong Hwa
BH