Antioxidant, free radical scavenging activities of Salvia brachyantha and its protective effect against oxidative cardiac cell injury.
In this study, antioxidant and free radical scavenging activities of an endemic Salvia species (Salvia brachyantha (Bordz) probed. was assessed in vitro using 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, beta-carotene linoleic acid, superoxide anion radical, hydroxyl radical, and reducing power assays. Regarding our data, the plant extract exhibited antioxidant and radical scavenging activities at different magnitudes of potency. In addition, this study was undertaken to assess whether methanol extract of S. brachyantha could increase the endogenous antioxidant enzymes in cells, and where such increased cellular defences could provide protection against oxidative cell injury. Pre treatment of rat heart cell lines with 100 microg/ml of plant extract for 24h significantly prevented cell damage and enhanced activity of antioxidant enzymes induced by a treatment with xanthine/xanthine oxidase. Increased reactive oxygen species and cell apoptosis induced by xanthine/xanthine oxidase was dose-dependently prevented when cells were pre treated for 24h with plant extract. These results indicated that S. brachyantha could protect against cell injury via induction of the antioxidant enzyme defences. The extract of this plant might be valuable antioxidant natural sources and seemed to be applicable in both healthy medicine and food industry.
Esmaeili
Mohammad Ali
MA
Department of Biology, Medicinal Plants and Drug Research Institute, Shahid Beheshti University GC, Tehran, Iran. m_esmaeili@sbu.ac.ir
Sonboli
Ali
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007492:Iran; D019787:Linoleic Acid / Q000737:chemistry; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D016877:Oxidants / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D019800:Phenol / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates; D020011:Protective Agents; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D019163:Reducing Agents; D027544:Salvia / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; D020011:Protective Agents; D017382:Reactive Oxygen Species; D019163:Reducing Agents; D013481:Superoxides; D019800:Phenol; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase