title: Understanding the molecular basis of plant growth promotional effect of Pseudomonas fluorescens on rice through protein profiling.
abstract: BACKGROUND
  Plant Growth Promoting Rhizobacteria (PGPR), Pseudomonas fluorescens strain KH-1 was found to exhibit plant growth promotional activity in rice under both in-vitro and in-vivo conditions. But the mechanism underlying such promotional activity of P. fluorescens is not yet understood clearly. In this study, efforts were made to elucidate the molecular responses of rice plants to P. fluorescens treatment through protein profiling. Two-dimensional polyacrylamide gel electrophoresis strategy was adopted to identify the PGPR responsive proteins and the differentially expressed proteins were analyzed by mass spectrometry.
  
  
  RESULTS
  Priming of P. fluorescens, 23 different proteins found to be differentially expressed in rice leaf sheaths and MS analysis revealed the differential expression of some important proteins namely putative p23 co-chaperone, Thioredoxin h- rice, Ribulose-bisphosphate carboxylase large chain precursor, Nucleotide diPhosphate kinase, Proteosome sub unit protein and putative glutathione S-transferase protein.
  
  
  CONCLUSIONS
  Functional analyses of the differential proteins were reported to be directly or indirectly involved in growth promotion in plants. Thus, this study confirms the primary role of PGPR strain KH-1 in rice plant growth promotion.
authors.affiliation: Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India.
authors.forename: Saveetha
authors.initials: S
authors.lastname: Kandasamy
authors.forename: Karthiba
authors.initials: K
authors.lastname: Loganathan
authors.forename: Raveendran
authors.initials: R
authors.lastname: Muthuraj
authors.forename: Saravanakumar
authors.initials: S
authors.lastname: Duraisamy
authors.forename: Suresh
authors.initials: S
authors.lastname: Seetharaman
authors.forename: Raguchander
authors.initials: R
authors.lastname: Thiruvengadam
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Ponnusamy
authors.forename: Samiyappan
authors.initials: S
authors.lastname: Ramasamy