Activation of human NK cells by malaria-infected red blood cells.
This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.
Horowitz
Amir
A
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.
Riley
Eleanor M
EM
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D042541:Intracellular Space / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008280:Magnetics; D010641:Phenotype; D010963:Plasmodium falciparum / Q000276:immunology*; D013194:Staining and Labeling; D015854:Up-Regulation
D015703:Antigens, CD; D007371:Interferon-gamma