title: A Streptococcus pyogenes derived collagen-like protein as a non-cytotoxic and non-immunogenic cross-linkable biomaterial.
abstract: A range of bacteria have been shown to contain collagen-like sequences that form triple-helical structures. Some of these proteins have been shown to form triple-helical motifs that are stable around body temperature without the inclusion of hydroxyproline or other secondary modifications to the protein sequence. This makes these collagen-like proteins particularly suitable for recombinant production as only a single gene product and no additional enzyme needs to be expressed. In the present study, we have examined the cytotoxicity and immunogenicity of the collagen-like domain from Streptococcus pyogenes Scl2 protein. These data show that the purified, recombinant collagen-like protein is not cytotoxic to fibroblasts and does not elicit an immune response in SJL/J and Arc mice. The freeze dried protein can be stabilised by glutaraldehyde cross-linking giving a material that is stable at >37 degrees C and which supports cell attachment while not causing loss of viability. These data suggest that bacterial collagen-like proteins, which can be modified to include specific functional domains, could be a useful material for medical applications and as a scaffold for tissue engineering.
authors.affiliation: CSIRO Molecular and Health Technologies, Bayview Avenue, Clayton, Victoria 3169, Australia.
authors.forename: Yong Y
authors.initials: YY
authors.lastname: Peng
authors.forename: Ayumi
authors.initials: A
authors.lastname: Yoshizumi
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Danon
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.forename: Olga
authors.initials: O
authors.lastname: Prokopenko
authors.forename: Oleg
authors.initials: O
authors.lastname: Mirochnitchenko
authors.forename: Zhuoxin
authors.initials: Z
authors.lastname: Yu
authors.forename: Masayori
authors.initials: M
authors.lastname: Inouye
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister
authors.forename: Barbara
authors.initials: B
authors.lastname: Brodsky
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005976:Glutaral / Q000494:pharmacology; D007114:Immunization; D051379:Mice; D055550:Protein Stability / Q000187:drug effects; D017434:Protein Structure, Tertiary; D012995:Solubility / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; C423453:Scl2 protein, Streptococcus; D003094:Collagen; D005976:Glutaral