Rapid, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of topically applied azithromycin in rabbit conjunctiva tissues.
A simple, rapid, sensitive and selective liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of azithromycin in rabbit conjunctiva tissues using roxithromycin as internal standard. Following a deproteinization procedure, the samples were eluted isocratically at a flow rate of 0.3mL/min utilizing a mobile phase containing of 10mM ammonium acetate (adjusted pH to 5.2 with 0.1% acetic acid)-methanol (18:82, v/v) and a SHISEIDO CAPCELL PAK C(18) (3.0mmx75mm, 3microm). Azithromycin and its internal standard were measured by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode with precursor-to-product qualifier transition m/z 375 [M+2H](2+)-->591 and m/z 837 [M+H](+)-->679 respectively. The method demonstrated that good linearity ranged from 10 to 2000ng/mL with r=0.9998. The lower limit of quantification for azithromycin in conjunctiva tissues was 10ng/mL with good accuracy and precision. The intra- and inter-day precision (RSD) values were below 15% and accuracy (%) ranged from 90% to 110% at all QC levels. The method was applicable to ocular pharmacokinetic studies of azithromycin.
Shen
Yan
Y
Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
Yin
Chun
C
Su
Mengxiang
M
Tu
Jiasheng
J
D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D017963:Azithromycin / Q000008:administration & dosage / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid / Q000592:standards; D003228:Conjunctiva / Q000737:chemistry*; D004355:Drug Stability; D019370:Observation; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
D000900:Anti-Bacterial Agents; D002021:Buffers; D017963:Azithromycin