title: Herpes simplex virus type-2 in Egyptian patients with bladder cancer or cystitis.
abstract: The present study was designed to investigate the prevalence of herpes simplex virus type-2 (HSV-2) in Egyptian patients with bladder cancer or cystitis and to evaluate the performance of different diagnostic HSV-2 assays. The study included 50 patients: 27 with bladder cancer (group I), 23 with cystitis (group II) and 20 subjects as controls (group III). HSV-2 DNA was detected using polymerase chain reaction (PCR) on bladder tissue and buffy coat cells (BCC). Electron microscopic studies (EMS) on BCC and ELISAs for IgM, IgG and specific glycoprotein G-2 (gG-2) IgG were performed. HSV-2 DNA was detected by PCR on bladder tissue biopsies in 29.6% and 21.7% of group I and II respectively and it was also detected by PCR on BCC in 22.2% and 21.7% of group I and II respectively. EMS revealed HSV like particles in 16.6% of cases. IgG, specific gG-2 IgG and IgM were detected in 30%, 16% and 6% of cases respectively. The different assays were evaluated in relation to PCR on bladder tissue biopsies. The gG-2-based ELISA and EMS on BCC were found to be highly specific (97.3% and 100% respectively), with similar low sensitivity of approximately 54%. PCR on BCC was the most sensitive assay. The association of HSV-2 with bladder cancer is suggested especially in schistosomal patients.
authors.affiliation: Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt.
authors.forename: Hala
authors.initials: H
authors.lastname: Badawi
authors.forename: Hanem
authors.initials: H
authors.lastname: Ahmed
authors.forename: Laila
authors.initials: L
authors.lastname: Aboul Fadl
authors.forename: Amira
authors.initials: A
authors.lastname: Helmi
authors.forename: Nevine
authors.initials: N
authors.lastname: Fam
authors.forename: Manal
authors.initials: M
authors.lastname: Diab
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ismail
authors.forename: Afkar
authors.initials: A
authors.lastname: Badawi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Saber
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001706:Biopsy; D003556:Cystitis / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D005260:Female; D006561:Herpes Simplex / Q000150:complications* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D001749:Urinary Bladder Neoplasms / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral