title: Rift Valley fever virus subunit vaccines confer complete protection against a lethal virus challenge.
abstract: Rift Valley fever virus (RVFV) is an emerging mosquito-borne virus causing significant morbidity and mortality in livestock and humans. Rift Valley fever is endemic in Africa, but also outside this continent outbreaks have been reported. Here we report the evaluation of two vaccine candidates based on the viral Gn and Gc envelope glycoproteins, both produced in a Drosophila insect cell expression system. Virus-like particles (VLPs) were generated by merely expressing the Gn and Gc glycoproteins. In addition, a soluble form of the Gn ectodomain was expressed and affinity-purified from the insect cell culture supernatant. Both vaccine candidates fully protected mice from a lethal challenge with RVFV. Importantly, absence of the nucleocapsid protein in either vaccine candidate facilitates the differentiation between infected and vaccinated animals using a commercial recombinant nucleocapsid protein-based indirect ELISA.
authors.affiliation: Central Veterinary Institute of Wageningen University and Research Centre, Cluster of Mammalian Virology, Edelhertweg 15, 8219 PH, Lelystad, The Netherlands. matthijn.deboer@wur.nl
authors.forename: S M
authors.initials: SM
authors.lastname: de Boer
authors.forename: J
authors.initials: J
authors.lastname: Kortekaas
authors.forename: A F
authors.initials: AF
authors.lastname: Antonis
authors.forename: J
authors.initials: J
authors.lastname: Kant
authors.forename: J L
authors.initials: JL
authors.lastname: van Oploo
authors.forename: P J M
authors.initials: PJ
authors.lastname: Rottier
authors.forename: R J M
authors.initials: RJ
authors.lastname: Moormann
authors.forename: B J
authors.initials: BJ
authors.lastname: Bosch
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D004330:Drosophila; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012295:Rift Valley Fever / Q000276:immunology / Q000517:prevention & control*; D012296:Rift Valley fever virus / Q000276:immunology*; D016019:Survival Analysis; D022223:Vaccines, Subunit / Q000276:immunology; D022721:Vaccines, Virosome / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D022223:Vaccines, Subunit; D022721:Vaccines, Virosome; D014759:Viral Envelope Proteins; D014765:Viral Vaccines