title: In vivo and in vitro polycyclic aromatic hydrocarbons degradation by Lentinus (Panus) tigrinus CBS 577.79.
abstract: The ability of stationary and shaken Lentinus tigrinus CBS 577.79 liquid cultures to degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) in N-rich (i.e., malt extract glucose, MEG) and in N-limited (low-N Kirk's medium, LNKM) media was investigated. Best results were obtained in shaken cultures where PAHs were degraded by 91% and 97% in MEG and LNKM, respectively; in stationary cultures, on the contrary, the degradation was never higher than 50%. Laccase activity was predominant on MEG while Mn-peroxidase (MnP) was preferentially produced in LNKM. The identification of degradation products showed the presence of several PAH derivatives, such as quinones, dicarboxylated and ring fission derivatives, presumably derived from the action of lignin-modifying enzymes. The presence of some degradation products (e.g., hydroxylated derivatives of anthrone and phenanthrene 9,10-dihydrodiol) suggested the possible involvement of cytochrome P-450-epoxide hydrolase system, the active form of which was found in 7-day-old cultures on MEG. In vitro experiments showed that the MnP from L. tigrinus had wider PAH substrate range and higher oxidation ability than the laccase produced by the same strain.
authors.affiliation: Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo De Lellis s.n.c., 01100 Viterbo, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Covino
authors.forename: Katerina
authors.initials: K
authors.lastname: Svobodová
authors.forename: Zdena
authors.initials: Z
authors.lastname: Kresinová
authors.forename: Maurizio
authors.initials: M
authors.lastname: Petruccioli
authors.forename: Federico
authors.initials: F
authors.lastname: Federici
authors.forename: Alessandro
authors.initials: A
authors.lastname: D'Annibale
authors.forename: Monika
authors.initials: M
authors.lastname: Cvancarová
authors.forename: Tomás
authors.initials: T
authors.lastname: Cajthaml
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004583:Electrons; D042845:Laccase / Q000378:metabolism; D020074:Lentinula / Q000201:enzymology / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D003577:Cytochrome P-450 Enzyme System; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase