title: Synthesis and gene delivery of poly(amido amine)s with different branched architecture.
abstract: A general strategy to improve the transfection efficiency as well as lower the cytotoxicity for polycationic vectors has been developed. Through the polycondensation addition of N,N'-methylene bisacrylamide and 1-(2-aminoethyl)piperazine in a water/N,N-dimethylformamide cosolvent, a series of cationic poly(amido amine)s with same repeating units but different branched architecture have been prepared. With the increase in branched architecture, the cationic polymers become more and more compact, accompanied by the enhancement of primary and tertiary amino groups. Therefore, the buffering capacities and DNA condensation capabilities of cationic poly(amido amine)s are strengthened greatly, whereas the correspondent cytotoxicity decreases. Correspondingly, the transfection efficiency is improved by more than three orders of magnitude. The results of this study indicate that the gene delivery can be readily regulated by only changing the branched architecture of polycations.
authors.affiliation: Shanghai Jiao Tong University, P. R. China.
authors.forename: Ruibin
authors.initials: R
authors.lastname: Wang
authors.forename: Linzhu
authors.initials: L
authors.lastname: Zhou
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guolin
authors.initials: G
authors.lastname: Li
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.forename: Xulin
authors.initials: X
authors.lastname: Jiang
authors.forename: Huiqin
authors.initials: H
authors.lastname: Li
authors.forename: Jieli
authors.initials: J
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Xinqiu
authors.initials: X
authors.lastname: Guo
authors.forename: Bangshang
authors.initials: B
authors.lastname: Zhu
authors.forename: Deyue
authors.initials: D
authors.lastname: Yan
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018014:Gene Transfer Techniques; D006801:Humans; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011073:Polyamines / Q000008:administration & dosage* / Q000138:chemical synthesis*
chemical_list: C531249:Poly(amidoamine); D011073:Polyamines