Cellular evaluation of synthesized insulin/transferrin bioconjugates for oral insulin delivery using intelligent complexation hydrogels.
Insulin transport across the epithelial cell layer in the small intestine was studied using insulin/transferrin conjugates with and without the presence of P(MAA-g-EG) microparticles in contact with a co-culture of Caco-2/HT29-MTX cells. The insulin/transferrin conjugate was shown to increase transport relative to pure insulin by a factor of 7, achieving an apparent permeability of 37 x 10(9) cm . s(-1). The presence of P(MAA-g-EG) microparticles increased conjugate transport by a factor of 14 times relative to insulin, achieving an apparent permeability of 72.8 x 10(9) cm . s(-1). The presence of the microparticles in solution was found to improve conjugate transport by nearly 100% with little to no change in cell monolayer integrity.
Shofner
Justin P
JP
Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Phillips
Margaret A
MA
Peppas
Nicholas A
NA
D000284:Administration, Oral; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D055252:Cell-Derived Microparticles / Q000378:metabolism; D018920:Coculture Techniques; D016503:Drug Delivery Systems; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D021381:Protein Transport; D012996:Solutions; D014168:Transferrin / Q000378:metabolism*
D020100:Hydrogels; D007328:Insulin; D011109:Polymethacrylic Acids; D012996:Solutions; D014168:Transferrin; C030613:polymethacrylic acid; D011092:Polyethylene Glycols