title: Protective effect of an herbal preparation (HemoHIM) on radiation-induced intestinal injury in mice.
abstract: The protective properties of an herbal preparation (HemoHIM) against intestinal damage were examined by evaluating its effects on jejunal crypt survival, morphological changes, and apoptosis in gamma-irradiated mice. The mice were whole-body irradiated with 12 Gy for the examination of jejunal crypt survival and any morphological changes and with 2 Gy for the detection of apoptosis and Ki-67 labeling. Irradiation was conducted using (60)Co gamma-rays. HemoHIM treatment was administered intraperitonially at a dosage of 50 mg/kg of body weight at 36 and 12 hours pre-irradiation and 30 minutes post-irradiation or orally at a dosage of 250 mg/kg of body weight/day for 7 or 11 days before necropsy. The HemoHIM-treated group displayed a significant increase in survival of jejunal crypts, when compared to the irradiation controls. HemoHIM treatment decreased intestinal morphological changes such as crypt depth, villus height, mucosal length, and basal lamina length of 10 enterocytes after irradiation. Furthermore, the administration of HemoHIM protected intestinal cells from irradiation-induced apoptosis. These results suggested that HemoHIM may be therapeutically useful to reduce intestinal injury following irradiation.
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Hae June
authors.initials: HJ
authors.lastname: Lee
authors.forename: Joong Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Jong Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Hae-Ran
authors.initials: HR
authors.lastname: Park
authors.forename: Uhee
authors.initials: U
authors.lastname: Jung
authors.forename: Jong Sik
authors.initials: JS
authors.lastname: Jang
authors.forename: Sung Kee
authors.initials: SK
authors.lastname: Jo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005720:Gamma Rays; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000293:injuries* / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D028321:Plant Preparations / Q000008:administration & dosage*; D011832:Radiation Injuries / Q000188:drug therapy* / Q000473:pathology; D011837:Radiation-Protective Agents / Q000008:administration & dosage*
chemical_list: D028321:Plant Preparations; D011837:Radiation-Protective Agents