Mycotoxin Ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana.
We evaluated the phytotoxicity of mycotoxin ochratoxin A (OTA) from Aspergillus and Penicillium strains on Arabidopsis thaliana. The results demonstrate that the growth of Arabidopsis thaliana on media containing OTA was inhibited significantly. Moreover, OTA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response lesions that are activated during plant-pathogen interactions and other abiotic stress factors. From our study, we can see that OTA exposure stimulated a biphasic oxidative burst in the leaves, resulting in the generation of hydrogen peroxide (H2O2) and superoxide anion radicals (O2(.-)) and in the concomitant down-regulation of antioxidant enzyme defense responses and up-regulation of lipid peroxidation. These results suggested that OTA damage might result from reactive oxygen species pathways. Our experiments provide a useful model plant system for research on OTA-induced plant cell death.
Peng
Xiao-Li
XL
Laboratory of Fruit Physiology and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, 100083 Beijing, China.
Xu
Wen-Tao
WT
Wang
Yan
Y
Huang
Kun-Lun
KL
Liang
Zhi-Hong
ZH
Zhao
Wei-Wei
WW
Luo
Yun-Bo
YB
D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation; D009793:Ochratoxins / Q000494:pharmacology*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*
D009793:Ochratoxins; D013481:Superoxides; C025589:ochratoxin A; D006861:Hydrogen Peroxide