Expressions of angiogenesis associated matrix metalloproteinases and extracellular matrix proteins in cerebral vascular malformations.
This study aimed to compare cerebral arteriovenous malformations (cAVM) and cerebral cavernous malformations (CCM) with regard to the immunohistochemical expressions of matrix metalloproteinases (MMP) and selected extracellular matrix (ECM) proteins, which have a role in the regulation of angiogenesis. Fresh-frozen surgical specimens from patients with cAVM (n=14) and CCM (n=15) were immunohistochemically stained with antibodies for MMP-2, MMP-9, laminin, fibronectin and tenascin. To compare cAVM and CCM, expression of each protein was graded using a four-point scoring system for each histological layer of the lesion. MMP-2 and MMP-9 were more strongly expressed in the vascular walls of CCMs compared to cAVMs for all comparable layers: endothelium, subendothelium and the perivascular space. The stronger expression of MMP and other EMP associated with early angiogenesis in CCMs compared to AVMs may support the hypothesis that CCMs occur at earlier embryogenic stages than AVMs.
Bicer
Atilla
A
Laboratory of Molecular Neurosurgery, Institute of Neurological Sciences, Marmara University, Istanbul 34853, Turkey.
Guclu
Bulent
B
Ozkan
Abdulkadir
A
Kurtkaya
Ozlem
O
Koc
Demet Yalcinkaya
DY
Necmettin Pamir
M
M
Kilic
Turker
T
D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002536:Cerebral Arteries / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology; D002550:Cerebral Veins / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism*; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D002538:Intracranial Arteriovenous Malformations / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D019063:Tenascin / Q000032:analysis / Q000378:metabolism
D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019063:Tenascin; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9