title: [A novel formulation of cisplatin (CDDP)].
abstract: The study aimed to assess our newly devised CDDP delivery system designed to provide targeting potential and a sustained release of the anticancer agent. Seventy percent deacetylated chitin was applied as a drug carrier. We prepared two different types of systems with two different procedures: namely, system A with direct method and system B with indirect method. The targeting property of the system was evaluated ex vivo with measuring adhesive force between each system and human colonic mucosa. The release behavior of the CDDP from the system was examined in vitro. The anticancer activities of the released CDDP were also examined in vitro using human gastric cancer cell line, MKN-45. Each system was a viscose elastic solution. The adhesive forces of the novel systems were stronger at 37 degrees C than that of 25 degrees C. Each system provided a sustained release of CDDP. The released CDDP demonstrated effective growth suppression activity against the MKN-45 cancer cells. The novel systems basically showed a favorable targeting function and a sustained release of CDDP, which effectively provided a growth inhibition potential against human cancer cell line. Our newly devised CDDP delivery systems are promising as a novel approach to cancer chemotherapy.
authors.affiliation: Department of Surgery, Iwate Medical University.
authors.forename: Akio
authors.initials: A
authors.lastname: Sugitachi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kimura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Itabashi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Noda
authors.forename: Kazushige
authors.initials: K
authors.lastname: Ishida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishizuka
authors.forename: Koki
authors.initials: K
authors.lastname: Otsuka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kashiwaba
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nitta
authors.forename: Keisuke
authors.initials: K
authors.lastname: Koeda
authors.forename: Masaru
authors.initials: M
authors.lastname: Mizuno
authors.forename: Akira
authors.initials: A
authors.lastname: Sasaki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ikeda
authors.forename: Yoshimori
authors.initials: Y
authors.lastname: Takamori
authors.forename: Go
authors.initials: G
authors.lastname: Wakabayashi
mesh_terms: D000268:Adhesiveness; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002686:Chitin; D002945:Cisplatin / Q000008:administration & dosage* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D002686:Chitin; D002945:Cisplatin