Analysis of loss of heterozygosity on chromosome 4q in hepatocellular carcinoma using high-throughput SNP array.
To identify tumour suppressor genes (TSGs) associated with hepatocellular carcinoma (HCC) on chromosome 4q using a high-throughput single nucleotide polymorphism (SNP) array, we first scanned for loss of heterozygosity (LOH) of 40 SNPs on chromosome 4q and discovered 2 hot regions: 4q24-26 and 4q34.3-35. We then further scanned for LOH of 338 SNPs in genes around 4q34.3-35 and discovered 3 genes with the most frequent LOH: nei endonuclease VIII-like 3 (NEIL3), interferon regulatory factor 2 (IRF2) and inhibitor of growth family member 2 (ING2). A review of the literature indicates only ING2 might be a TSG associated with HCC.
Zhang
Huakun
H
State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangdong, PR China.
Ma
Haiqing
H
Wang
Qijing
Q
Chen
Minshan
M
Weng
Desheng
D
Wang
Hui
H
Zhou
Jun
J
Li
Yongqiang
Y
Sun
Jiancong
J
Chen
Yibing
Y
Liang
Xiaoting
X
Zhao
Jingjing
J
Pan
Ke
K
Wang
Huiyun
H
Xia
Jianchuan
J
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002894:Chromosomes, Human, Pair 4; D015331:Cohort Studies; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D016147:Genes, Tumor Suppressor; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult