title: Acquired immune response to defined Plasmodium vivax antigens in individuals residing in northern India.
abstract: In this investigation, we evaluated the naturally acquired immune response to Plasmodium vivax stage-specific antigens in individuals of different age groups belonging to malaria endemic areas of northern India. Four synthetic peptides containing both B- and T-cell epitopes from P. vivax circumsporozoite protein, merozoite surface protein-1, apical membrane antigen-1 and gametocyte surface antigen-1 were used to determine both humoral and cellular immune responses. Immunity, in terms of antibody response and T-cell proliferation against these stage-specific peptides, has been observed in the study subjects. The results demonstrated age-dependent antibody response in this population. Forty two patients were diagnosed with P. vivax. There was a significant association (P=0.013) between number of antibody responders and recognition of stage-specific epitopes by antibodies. The antibody response to B-epitopes of P. vivax CSP, MSP1, AMA1 and GAM1 was associated with age; adults responded more frequently to these antigens than did younger children. In this population, 66% (201/304) cases showed seropositivity to all peptides and 13% (41/304) showed negative response. Peripheral blood mononuclear cells of more than 75% of individuals proliferated in response to stimulation by all four epitopes. In conclusion, the results demonstrated immunogenicity of the epitopes to P. vivax in population of this endemic zone.
authors.affiliation: National Institute of Malaria Research (Indian Council of Medical Research), Sector 8, Dwarka, New Delhi 110077, India.
authors.forename: Ratanesh K
authors.initials: RK
authors.lastname: Seth
authors.forename: Ajaz A
authors.initials: AA
authors.lastname: Bhat
authors.forename: Donthamsetty N
authors.initials: DN
authors.lastname: Rao
authors.forename: Sukla
authors.initials: S
authors.lastname: Biswas
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D019353:Endemic Diseases; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D016780:Malaria, Vivax / Q000453:epidemiology / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D015800:Protozoan Proteins