Polyhydroxyalkanoate (PHA) synthesis by Spirulina subsalsa from Gujarat coast of India.
Cyanobacteria have many unexploited potential for natural products with a huge variability in structure and biological activity. Their products are species specific and substrate+growth condition specific. Under stress conditions they are reported to produce biopolymers like EPS and PHA, which can be produced extracellularly and intracellularly, respectively. Polyhydroxyalkanoates are polymers of biological origin, they are also capable of being completely broken down to water and carbon dioxide by microorganisms found in a wide range of environments, such as soil, water, and sewage. We have studied marine cyanobacteria Spirulina subsalsa from Veraval coast, Gujarat, India, producing PHA under increased sodium chloride (NaCl) concentration (5% enhancement to the ASNIII medium), The biopolymer was chemically characterized through FTIR, NMR, TGA, and DSC. The present study shows increased PHA accumulation in S. subsalsa by twofold increased NaCl concentration in the growth media.
Shrivastav
Anupama
A
Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research, G.B. Marg, Bhavnagar 364021, India.
Mishra
Sanjiv K
SK
Mishra
Sandhya
S
D002152:Calorimetry, Differential Scanning; D007194:India; D009682:Magnetic Resonance Spectroscopy; D010078:Oxazines; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D053545:Spirulina / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling; D013818:Thermogravimetry
D010078:Oxazines; D054813:Polyhydroxyalkanoates; D012965:Sodium Chloride; C044808:nile red