Studies on plasmalogen-selective phospholipase A2 in brain.
Plasmalogen-selective phospholipase A(2) (PlsEtn-PLA(2)) has been purified from pig brain using multiple column chromatographic procedure. The purified enzyme migrates as a single band on polyacrylamide. It is stimulated by Triton X-100 and inhibited by sodium deoxycholate. Purified PlsEtn-PLA(2) is inhibited by iodoacetate, and this inhibition can be prevented by beta-meracaptoethanol. Treatment of neuronal cell cultures with kainic acid stimulates PlsEtn-PLA(2) activity in a dose-dependent manner, and this stimulation can be blocked by Ly294486, a selective kainic acid receptor antagonist. Activities of PlsEtn-PLA(2) are markedly increased in plasma membrane and synaptosomal plasma membrane fraction prepared from nucleus basalis and hippocampal region of brains from Alzheimer disease patients compared to age-matched controls. It is proposed that accumulation of ceramide and increased expression of cytokines may be responsible for the stimulation of PlsEtn-PLA(2) in Alzheimer disease.
Farooqui
Akhlaq A
AA
Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210, USA. farooqui.1@osu.edu
D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002478:Cells, Cultured; D003902:Detergents / Q000737:chemistry; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006801:Humans; D007608:Kainic Acid / Q000494:pharmacology; D008055:Lipids / Q000737:chemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017830:Octoxynol / Q000737:chemistry; D054467:Phospholipases A2 / Q000302:isolation & purification / Q000378:metabolism*; D010955:Plasmalogens / Q000378:metabolism
D003902:Detergents; D018690:Excitatory Amino Acid Agonists; D008055:Lipids; D010955:Plasmalogens; D017830:Octoxynol; D054467:Phospholipases A2; C117522:plasmalogen-selective phospholipase A2; D007608:Kainic Acid