Time-dependent organic changes of intravenous thrombi in stasis-induced deep vein thrombosis model and its application to thrombus age determination.
Using histochemical and immunohistochemical techniques, we examined the intrathrombotic collagen contents and the appearance of hemosiderin-positive cells, neovessels, and myofibroblasts in a stasis-induced venous thrombosis model. The intrathrombotic collagen deposition area occupied about 20% at 5 days, and exceeded 80% at 21 days after ligation of the inferior vena cava (IVC). Hemosiderin-positive cells in the thrombus first appeared at 3 days in only one of the five samples, and positive cells were constantly detected in all thrombi at 5 days or later. CD31-positive neovessels in the thrombus first appeared at 5 days in one of five samples and were detected in all samples after 10 days. At 7 days, alphaSMA-positive myofibroblasts at the periphery of the thrombus first appeared in three of five samples, and were detected and enhanced time-dependently in all samples after 10 days. These observations demonstrated that these markers would be applicable for thrombus age determination.
Nosaka
Mizuho
M
Department of Forensic Medicine, Wakayama Medical University, Wakayama, Japan.
Ishida
Yuko
Y
Kimura
Akihiko
A
Kondo
Toshikazu
T
D000818:Animals; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000473:pathology; D049429:Forensic Pathology; D006485:Hemosiderin / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011180:Postmortem Changes; D013997:Time Factors; D014682:Vena Cava, Inferior / Q000473:pathology; D020246:Venous Thrombosis / Q000378:metabolism / Q000473:pathology*
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D003094:Collagen; D006485:Hemosiderin