title: The genome-wide expression profile of electroacupuncture in DNP-KLH immunized mice.
abstract: Previously, we demonstrated that electoracupuncture (EA) suppressed allergic reactions in DNP-KLH immunized mice. In this study, the mechanisms by which EA induces immunomodulation in the immunized mice were evaluated by genome-wide microarray analysis. The anti-allergic effects of EA in DNP-KLH immunized mice were confirmed by analyzing antigen specific IgE using ELISA. Microarray analysis, followed by real time RT-PCR validation, revealed that Th1 and Th17 cytokine-, opioid peptide-, and anti-apoptosis-related genes were up-regulated upon treatment with EA. In addition, significant decreases in Th2 cytokine-, MAPK signaling pathway-, and apoptosis-related genes were observed following EA treatment.
authors.affiliation: Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, 130-701, Republic of Korea.
authors.forename: Sung-Hwa
authors.initials: SH
authors.lastname: Sohn
authors.forename: Sun Kwang
authors.initials: SK
authors.lastname: Kim
authors.forename: Eunjung
authors.initials: E
authors.lastname: Ko
authors.forename: Youngseop
authors.initials: Y
authors.lastname: Lee
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Hyojung
authors.initials: H
authors.lastname: Lee
authors.forename: Hyunseong
authors.initials: H
authors.lastname: Kim
authors.forename: Deok-Sang
authors.initials: DS
authors.lastname: Hwang
authors.forename: Sangsoo
authors.initials: S
authors.lastname: Nam
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D019297:2,4-Dinitrophenol / Q000276:immunology; D000276:Adjuvants, Immunologic; D000818:Animals; D016207:Cytokines / Q000276:immunology; D015671:Electroacupuncture; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000628:therapy*; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D015203:Reproducibility of Results; D014475:Uncoupling Agents
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D014475:Uncoupling Agents; D007073:Immunoglobulin E; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D019297:2,4-Dinitrophenol