title: Characterization and expression of cry4Cb1 and cry30Ga1 from Bacillus thuringiensis strain HS18-1.
abstract: We characterized a novel Bacillus thuringiensis isolate native to China (HS18-1) that shows a spherical crystal harboring two major proteins of about 70 and 130kDa, and contains three novel cry genes (cry4Cb1, cry30Ga1, cry54-type). Furthermore, the cry4Cb1 and cry30Ga1 genes were expressed in Escherichia coli BL21 (DE3): pLysS. Insecticidal activity tests showed that the cry4Cb1 protein exhibited larvicidal activity against Aedes aegypti (Diptera) and the cry30Ga1 protein was toxic to both A. aegypti and P. xylostella (Lepidoptera).
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Aiping
authors.initials: A
authors.lastname: Zheng
authors.forename: Shiquang
authors.initials: S
authors.lastname: Wang
authors.forename: Huainian
authors.initials: H
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008855:Microscopy, Electron, Scanning; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins