Identification of binding proteins of fusarielin A as actin and tubulin.
Fusarielin A (FSA), isolated from culture of Fusarium sp. K432, has anti-fungal/anti-angiogenic activity. Based on our previous structure-activity relationship studies, we prepared FSA-immobilized affinity gel. The results of affinity chromatography with this gel indicated that tubulin and actin are FSA-binding proteins. The binding affinities of tubulin and actin for FSA were estimated by the use of surface plasmon resonance spectroscopy.
Noguchi-Yachide
Tomomi
T
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Dodo
Kosuke
K
Aoyama
Hiroshi
H
Fujimoto
Haruka
H
Hori
Masatoshi
M
Hashimoto
Yuichi
Y
Kobayashi
Hisayoshi
H
D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000595:Amino Acid Sequence; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004852:Epoxy Compounds / Q000302:isolation & purification / Q000494:pharmacology; D005670:Fusarium / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000302:isolation & purification / Q000494:pharmacology; D011485:Protein Binding; D014404:Tubulin / Q000032:analysis / Q000378:metabolism*
D000199:Actins; D020533:Angiogenesis Inhibitors; D000935:Antifungal Agents; D004852:Epoxy Compounds; D009281:Naphthalenes; D014404:Tubulin; C092174:fusarielin A