title: Direct 16S rRNA gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms.
abstract: Two cases involving polymicrobial culture-negative samples were investigated by 16S rRNA gene sequencing, with analysis of mixed chromatograms. Fusobacterium necrophorum, Prevotella intermedia and Streptococcus constellatus were identified from pleural fluid in a patient with Lemierre's syndrome and Neisseria meningitidis and Escherichia coli were identified from a petechia in a patient with meningococcal disease.
authors.affiliation: Department of Clinical Microbiology, Odense University Hospital, Odense,Denmark.
authors.forename: Gitte N
authors.initials: GN
authors.lastname: Hartmeyer
authors.affiliation: Department of Clinical Microbiology, Odense University Hospital, Odense,Denmark.
authors.forename: Ulrik S
authors.initials: US
authors.lastname: Justesen
mesh_terms: D000293:Adolescent; D002845:Chromatography; D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000302:isolation & purification; D005675:Fusobacterium necrophorum / Q000302:isolation & purification; D020459:Genes, rRNA; D006801:Humans; D008297:Male; D008875:Middle Aged; D009345:Neisseria meningitidis / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S