[Pharmacological assessment of ARTCEREB irrigation and perfusion solution for cerebrospinal surgery based on glucose incorporation activity in primary cultures of rat brain cells].
ARTCEREB irrigation and perfusion solution (Artcereb) is typically used as an artificial fluid for applications inside the skull and spinal cavity. This in vitro study was conducted to assess the effects of Artcereb in cultures of rat fetal brain cells. Cell function following exposure to Artcereb was evaluated by measuring (3)H-deoxy-D-glucose incorporation activity. Cell function was significantly reduced in primary cultures of rat fetal brain cells at 0 h and 24 h after 1-h or 3-h exposure to normal saline as compared with Artcereb. Cell function was also significantly reduced at 24 h after 3-h exposure to lactated Ringer's solution as compared with Artcereb. Furthermore, cell function was significantly reduced at 24 h after 3-h exposure to a modified Artcereb formulation lacking either HCO(3)(-) or Mg(2+) as compared with Artcereb, while cell function was unaffected at 24 h after exposure to lactated Ringer's solution with HCO(3)(-) or normal saline with HCO(3)(-) as compared with Artcereb. These findings suggest the importance of the presence of HCO(3)(-) and Mg(2+) in the formulation of Artcereb.
Nishimura
Masuhiro
M
Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan. nisimums@otsukakj.co.jp
Doi
Kazuhisa
K
Naito
Shinsaku
S
D000818:Animals; D001639:Bicarbonates; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D005947:Glucose / Q000378:metabolism*; D008274:Magnesium; D019635:Neurosurgical Procedures; D010477:Perfusion; D019999:Pharmaceutical Solutions / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions; D007507:Therapeutic Irrigation; D013997:Time Factors
D001639:Bicarbonates; D019999:Pharmaceutical Solutions; D012996:Solutions; D008274:Magnesium; D005947:Glucose