Enzymatic reactions in near critical CO₂: The effect of pressure on phenol removal by tyrosinase.
The use of enzymes in supercritical CO(2) (SCCO(2)) has received extensive attention in recent years. Biocatalysts have the advantage of substrate specificity and SCCO(2) offers several advantages over liquid solvents. This work deals with the utilization of SCCO(2) as a medium for the enzymatic removal of phenol from aqueous solutions using tyrosinase. Since the presence of oxygen is crucial for the enzyme-catalyzed oxidation, the substantial solvating power of SCCO(2) makes it a promising medium for such reactions. The conversion of phenol was higher at 10 MPa. Under near critical conditions (7 MPa, 35 degrees C), the addition of air at 5 x 10(5) Pa of pressure improved phenol removal.
Amaral
Priscilla
P
School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
Garcia
Daniela
D
School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
Cardoso
Miguel
M
Chemical and Biological Engineering Department, Instituto Superior Técnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
Mendes
Marisa
M
Chemical Engineering Department, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, 23890, Seropédica, RJ, Brazil.
Coelho
Maria Alice
MA
School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
Pessoa
Fernando
F
School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
D000364:Agaricus / Q000201:enzymology; D002245:Carbon Dioxide / Q000737:chemistry*; D002384:Catalysis; D005656:Fungal Proteins / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D011312:Pressure
D005656:Fungal Proteins; D002245:Carbon Dioxide; D019800:Phenol; D014442:Monophenol Monooxygenase