[Effects of ShenSongYangXin on action potential and some current channels in isolated rabbit pulmonary vein cardiomyocytes].
OBJECTIVE
To investigate the effects of ShenSongYangXin on action potential and some current channels in isolated rabbit pulmonary vein cardiomyocytes and to elucidate its possible mechanism of curing atrial fibrillation.


METHODS
Action potentials as well as L-type calcium channel current (I(Ca-L)), transient outward potassium current (I(K1)) and inward rectifier potassium current (I(To)) were recorded using whole cell patch-clamp technique.


RESULTS
ShenSongYangXin prolonged the action potential duration (APD) significantly, APD90 was enlarged from (187 +/- 49) ms to (286 +/- 76) ms at the concentration of 5 mg/ml and to (312 +/- 82) ms at the concentration of 10 mg/dl respectively (P < 0.05). It also depressed the current of I(Ca-L), I(K1) and Iro in a concentration-dependent manner.


CONCLUSIONS
ShenSongYangXin can be used to treat atrial fibrillation through its actions on multiple current channels.
Shi
Liang
L
Heart Center, Beijing Chao-Yang Hospital, Capital University of Medical Sciences, Beijing 100020, China.
Yang
Xin-Chun
XC
Liu
Xiu-Lan
XL
Zong
Min
M
Wu
Yi-Ling
YL
D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D001281:Atrial Fibrillation; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D011667:Pulmonary Veins / Q000166:cytology; D011817:Rabbits
D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal; D024661:Potassium Channels, Inwardly Rectifying; C530959:shensongyangxin