Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals.
Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.
Clark
Jeremy J
JJ
Department of Psychiatry and Behavioral Sciences and Department of Pharmacology, University of Washington, Seattle, Washington, USA.
Sandberg
Stefan G
SG
Wanat
Matthew J
MJ
Gan
Jerylin O
JO
Horne
Eric A
EA
Hart
Andrew S
AS
Akers
Christina A
CA
Parker
Jones G
JG
Willuhn
Ingo
I
Martinez
Vicente
V
Evans
Scott B
SB
Stella
Nephi
N
Phillips
Paul E M
PE
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D004567:Electrodes, Implanted; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008636:Mesencephalon / Q000502:physiology*; D051379:Mice; D008839:Microelectrodes; D008904:Miniaturization; D018670:Monitoring, Ambulatory / Q000295:instrumentation*; D018377:Neurotransmitter Agents / Q000032:analysis; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018377:Neurotransmitter Agents; D004298:Dopamine