title: Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica.
abstract: Suppressor of cytokine signaling (SOCS) proteins are key physiological regulators of both innate and adaptive immunity. These proteins belong to the three major classes of modulators of cytokines signaling. In the following article, we used porcine polarized intestinal cells to study early response to the protozoan, Entamoeba histolytica, and we identified by rapid amplification of cDNA ends (RACE) PCR porcine SOCS1, SOCS4, SOCS5 and SOCS6 encoding sequences. With more than 92% identity predicted porcine SOCS proteins are very similar to their human counterparts. Among SOCS transcripts, only SOCS2 mRNA was significantly induced in epithelial intestinal cells in response to the cytolytic activity of the parasite. The transcriptomic profile obtained after 3h of co-culture of polarized intestinal cells with E. histolytica was clearly oriented toward inflammation and the recruitment of neutrophils. These transcriptomic data have been normalized with accuracy by the utilisation of multiple validated reference genes. The analysis offers a first set of reference genes useful for future studies in porcine intestinal cells. Our data shed light on the understanding of the early response of polarized intestinal cells to E. histolytica and identified a potential involvement of SOCS2 in the parasite regulation of the host response.
authors.affiliation: Institut National de la Recherche Agronomique, UR1282, Infectiologie Animale et Santé Publique, Nouzilly, Tours, France.
authors.forename: Timothée
authors.initials: T
authors.lastname: Bruel
authors.forename: Roseline
authors.initials: R
authors.lastname: Guibon
authors.forename: Sandrine
authors.initials: S
authors.lastname: Melo
authors.forename: Nancy
authors.initials: N
authors.lastname: Guillén
authors.forename: Henri
authors.initials: H
authors.lastname: Salmon
authors.forename: Fabienne
authors.initials: F
authors.lastname: Girard-Misguich
authors.forename: François
authors.initials: F
authors.lastname: Meurens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D004748:Entamoeba histolytica / Q000276:immunology* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology; D006790:Host-Parasite Interactions; D006801:Humans; D007249:Inflammation; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017386:Sequence Homology, Amino Acid; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013552:Swine
chemical_list: D050826:Suppressor of Cytokine Signaling Proteins