Molecular cloning and sequencing of the cDNAs encoding the bat interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12p40, and tumor necrosis factor-alpha.
This is the first report on the cDNA sequences of bat interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12 p40, and tumor necrosis factor (TNF)-alpha. The cDNAs of bat IL-2, IL-4, IL-6, IL-10, IL-12 p40, and TNF-alpha comprise 459, 405, 624, 537, 990, and 699 base pairs respectively. Moreover, each of the cDNAs of bat IL-2, IL-4, IL-6, IL-10, IL-12 p40, and TNF-alpha contain a single open reading frames encoding 152, 134, 207, 178, 329, and 232 amino acids, respectively. The comparison of bat cytokines with Perrissodactyla (horse), Carnivora (dog and cat), and Cetartiodactyla (cattle and pig) orthologs revealed a high degree of homology. Although the N-terminal amino acids and cysteine residues are highly conserved in each mature cytokine, the deduced N-linked glycosylation sites vary across species.
Iha
Koichiro
K
Department of Biomedical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. aa097127@mail.ecc.u-tokyo.ac.jp
Omatsu
Tsutomu
T
Watanabe
Shumpei
S
Ueda
Naoya
N
Taniguchi
Satoshi
S
Fujii
Hikaru
H
Ishii
Yoshiyuki
Y
Kyuwa
Shigeru
S
Akashi
Hiroomi
H
Yoshikawa
Yasuhiro
Y
D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics; D053762:Interleukin-12 Subunit p40 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4