Molecular characterization of class 1 integrons in MDR Pseudomonas aeruginosa isolated from clinical settings in Iran, Tehran.
Seven hundred and fifty nonreplicated clinical isolates of Pseudomonas aeruginosa were collected from five hospitals during March 2007 to May 2009. Forty-one isolates (5.46%) were characterized as multidrug-resistant P. aeruginosa (MDRPA). PCR assays were used to detect class 1 integrons. Amplifications of internal variable regions (IVRs) of class 1 integrons revealed three different arrays (0.8, 1.3, and 1.7 kb) with different distributions in clinical isolates. The amplified IVRs were sequenced and three gene cassette arrays including aadB (0.8 kb), aadA6-orfD (1.3 kb), and bla(OXA10)-aacA4 (1.7 kb) were identified. In conclusion, we confirmed the high prevalence of class 1 integons with limited diversity of gene cassette arrays in MDRPA clinical isolates found from five hospitals. This is the first report showing gene cassette contents of class 1 integrons in P. aeruginosa isolates in Iran.
Shahcheraghi
Fereshteh
F
Department of Microbiology, Pasteur Institute, Tehran, Iran. shahcheraghifereshteh@yahoo.com <shahcheraghifereshteh@yahoo.com>
Badmasti
Farzad
F
Feizabadi
Mohammad M
MM
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006761:Hospitals / Q000706:statistics & numerical data; D006801:Humans; D032023:Integrons / Q000235:genetics*; D007492:Iran; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial