Plasmid-borne florfenicol and ceftiofur resistance encoded by the floR and blaCMY-2 genes in Escherichia coli isolates from diseased cattle in France.
This study was designed to determine the genetic basis of florfenicol and ceftiofur resistance in Escherichia coli isolates recovered from French cattle. In these isolates, ceftiofur resistance was conferred by bla(CMY-2) located on three distinct conjugative plasmids on a specific DNA fragment, ISEcp1-bla(CMY-2)-blc- sugE. Two of the plasmids also carried the floR gene conferring resistance to florfenicol. The floR gene was shown to be associated with the insertion sequence ISCR2. Mobile elements appear to contribute to the mobilization of floR and bla(CMY-2) genes in E. coli. The presence of bla(CMY-2) and floR on the same plasmid highlights the potential risk for a co-selection of the bla(CMY-2) gene through the use of florfenicol in food animal production.
Meunier
Danièle
D
Agence Française de SécuritéSanitaire des Aliments, Unité Antibiorésistance et VirulenceBactériennes, 31 avenue Tony Garnier, 69364 Lyon, France.
Jouy
Eric
E
Agence Française de SécuritéSanitaire des Aliments, Unité Mycoplasmologie - Bactériologie,BP 53, 22440 Ploufragan, France.
Lazizzera
Corinne
C
Agence Française de SécuritéSanitaire des Aliments, Unité Antibiorésistance et VirulenceBactériennes, 31 avenue Tony Garnier, 69364 Lyon, France.
Doublet
Benoît
B
INRA, UR1282 Infectiologie Animale Santé Publique,37380 Nouzilly, France.
Kobisch
Marylène
M
Agence Française de SécuritéSanitaire des Aliments, Unité Mycoplasmologie - Bactériologie,BP 53, 22440 Ploufragan, France.
Cloeckaert
Axel
A
INRA, UR1282 Infectiologie Animale Santé Publique,37380 Nouzilly, France.
Madec
Jean-Yves
JY
Agence Française de SécuritéSanitaire des Aliments, Unité Antibiorésistance et VirulenceBactériennes, 31 avenue Tony Garnier, 69364 Lyon, France.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002511:Cephalosporins / Q000494:pharmacology*; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics*
D002511:Cephalosporins; D029968:Escherichia coli Proteins; C501927:FloR protein, E coli; C053503:ceftiofur; C035534:florfenicol; C103022:beta-lactamase CMY-2; D001618:beta-Lactamases; D013839:Thiamphenicol