title: Matrix metalloproteinase-9 reversibly affects the time course of NMDA-induced currents in cultured rat hippocampal neurons.
abstract: Matrix Metalloproteinase 9 (MMP-9) has been demonstrated to play a crucial role in maintenance of NMDA receptor-dependent LTP and in lateral mobility of these receptors. However, the effect of MMP-9 on NMDA receptor (NMDAR) functional properties is unknown. For this purpose we have investigated the impact of recombinant MMP-9 on the whole-cell NMDAR-mediated current responses in cultured hippocampal neurons. Treatment with MMP-9 induced a reversible acceleration of desensitization and deactivation kinetics but had no effect on current amplitude. Interestingly, phorbol ester, a PKC activator known to enhance NMDAR lateral mobility, induced kinetic changes of currents similar to those produced by MMP-9. In conclusion, our results show that MMP-9 reversibly modulates the NMDAR kinetics and raise a possibility that this modulation could be related to the lateral mobility of these receptors.
authors.affiliation: Department of Biophysics, Laboratory of Neuroscience, Wroclaw Medical University, ul.Chalubinskiego 3, 50-367 Wroclaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gorkiewicz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szczuraszek
authors.forename: Paulina
authors.initials: P
authors.lastname: Wyrembek
authors.forename: Piotr
authors.initials: P
authors.lastname: Michaluk
authors.forename: Leszek
authors.initials: L
authors.lastname: Kaczmarek
authors.forename: Jerzy W
authors.initials: JW
authors.lastname: Mozrzymas
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D020780:Matrix Metalloproteinase 9 / Q000494:pharmacology / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D020780:Matrix Metalloproteinase 9