title: Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells.
abstract: Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.
authors.affiliation: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Konishi
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Seiyei
authors.initials: S
authors.lastname: Yamakawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Iwai
authors.forename: Shinobu
authors.initials: S
authors.lastname: Satoh
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishii
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D019751:Intramolecular Transferases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D012275:Oryza / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology / Q000235:genetics*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014539:Uridine Diphosphate Sugars; C040817:uridine diphosphate arabinose; D019751:Intramolecular Transferases