mRNA expression of MDR1 and major metabolising enzymes in human fetal tissues.
Metabolising enzymes and transport proteins are largely expressed in human tissues. They have major impact on drug disposition and effects. We studied mRNA expression of phase I and II metabolising enzymes and transporters in fetal tissues at different development stages. Hepatic, duodenal, renal and neurological fetal tissues were studied at 15, 27 and 42 weeks gestation and mRNA expression of 84 enzymes and transporters was analysed by with the RT(2) Profiler PCR array system. There was wide variability in gene expression between different samples. Independently from age, the highest expression levels were observed in the liver and the lowest in the brain for the majority of genes tested. There was significant increase in gene expression with age in duodenal and hepatic tissues.
Fakhoury
May
M
Department of Paediatric Pharmacology and Pharmacogenetics, Robert Debré Hospital, 75935 Paris Cedex 19, France.
de Beaumais
Tiphaine
T
Guimiot
Fabien
F
Azougagh
Said
S
Elie
Valery
V
Medard
Yves
Y
Delezoide
Anne-Lise
AL
Jacqz-Aigrain
Evelyne
E
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000367:Age Factors; D005333:Fetus / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D004364:Pharmaceutical Preparations; D012333:RNA, Messenger