[Dynamic changes of serum MCP-1 and MIP-2 chemokines after renal transplantation in rats].
OBJECTIVE
To investigate the changes in serum levels of chemokine MCP-1 and MIP-2 after renal transplantation in rats and the influence of Cyclosporin A (CsA) on their levels.


METHODS
Three groups of rats, namely untreated group, CsA group and isograft group underwent the renal transplantation, and the serum MCP-1 and MIP-2 levels of the recipients were tested using enzyme-linked immunosorbent assay (ELISA).


RESULTS
The serum MCP-1 level peaked 6 hours after the operation and also in critical stages of acute rejection. The first peak of MCP-1 was postponed by the application of CsA, which did not affect the peak level (P>0.05). The second peak of MCP-1 did not occur in CsA-treated group. MIP-2 concentration also peaked at 6 h after the operation in all the groups. The second peak of MIP-2, which was lower than the first one (P<0.05), occurred 9 days after the transplantation.


CONCLUSIONS
MCP-1 and MIP-2 in involved not only in the ischemia-reperfusion injury but also in severe acute rejection. CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats.
Yao
Hao
H
Department of Urology, School of Clinical Medicine, Yangzhou University, Yangzhou 225001, China. yjj_xz@sina.com
Yu
Jun-jie
JJ
D000818:Animals; D018932:Chemokine CCL2 / Q000097:blood*; D054426:Chemokine CXCL2 / Q000097:blood*; D016572:Cyclosporine / Q000494:pharmacology*; D006084:Graft Rejection / Q000097:blood / Q000188:drug therapy; D016030:Kidney Transplantation / Q000009:adverse effects; D008297:Male; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D054426:Chemokine CXCL2; C513296:Cxcl2 protein, rat; D016572:Cyclosporine