title: Bacterial adhesion onto materials with specific surface chemistries under flow conditions.
abstract: Staphylococcus epidermidis adhesion onto materials with specific chemical functionalities, under flow, was investigated by using surfaces prepared by self-assembly of alkyl silane monolayers on glass. Terminal methyl (CH(3)) and amino (NH(2)) groups were formed by chemical vapor deposition of silanes, at elevated temperature. Carboxyl (COOH) terminated groups were prepared by further modification of NH(2) groups with succide anhydride and positively charged NH(2) groups by adsorption of poly-L: -lysine hydrobromide. Hydroxyl (OH) terminated glass was used as control. Surface modification was verified by contact angle measurements, atomic force microscopy and X-ray photoelectron spectroscopy. A parallel plate flow chamber was used to evaluate bacterial adhesion at various shear rates. Adhesion was found to be depended on the monolayer's terminal functionality. It was higher on the CH(3) followed by the positively charged NH(2), the non-charged NH(2) groups, the COOH and minimal on the OH-terminated glass. The increase in the material surface free energy significantly reduced the adhesion of a hydrophilic bacterial strain, and this is in accordance with the predictions of the thermodynamic theory. However, the increase in the shear rate restricted the predictability of the theory and revealed macromolecular interactions between bacteria and NH(2)- and COOH-terminated surfaces.
authors.affiliation: Laboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, Rion, 26504, Patras, Greece.
authors.forename: M G
authors.initials: MG
authors.lastname: Katsikogianni
authors.forename: Y F
authors.initials: YF
authors.lastname: Missirlis
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion; D001965:Bromides / Q000737:chemistry; D018929:Cell Culture Techniques / Q000295:instrumentation; D005898:Glass; D005990:Glycerol / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D011107:Polylysine / Q000737:chemistry; D012821:Silanes; D013212:Staphylococcus epidermidis / Q000378:metabolism; D013314:Stress, Mechanical; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D017155:Wettability
chemical_list: D001965:Bromides; D012821:Silanes; D014867:Water; D011107:Polylysine; D005990:Glycerol