Triazole-containing BODIPY dyes as novel fluorescent probes for soluble oligomers of amyloid Abeta1-42 peptide.
A straightforward functionalization of BODIPY dyes via incorporation of a triazole moiety produced fluorescent dyes that were capable of distinguishing between secondary structure conformations of soluble oligomeric species of Abeta1-42 peptide. Small concentrations of the dyes, relative to Abeta1-42, provided up to an 8-fold and 35-fold fluorescence increase in the presence of the unordered and ordered, beta-sheet-rich conformations of soluble Abeta1-42 oligomers, respectively. These triazole-containing dyes could prove to be useful probes for monitoring amyloid conformational transitions in vitro.
Smith
Nicholas W
NW
Department of Chemistry, Texas Christian University, Fort Worth, TX 76129, USA.
Alonso
Annabel
A
Brown
Christopher M
CM
Dzyuba
Sergei V
SV
D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D014230:Triazoles / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D016229:Amyloid beta-Peptides; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D014230:Triazoles; C075222:amyloid beta-protein (1-42)