Analysis of protein physical stability in lipid based delivery systems--the challenges of lipid drug delivery systems.
Lipid based systems are a promising delivery system for many drug molecules, including labile compounds such as peptides and proteins. However, in the development of these systems the effect on the physical stability is rarely taken into account. Physical changes are often correlated with changes in biological activity but are generally much easier to assess. The present paper illustrates the use of a combination of techniques, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and intrinsic tryptophan fluorescence, to determine structural changes of proteins in lipid based systems such as solid lipid nanoparticles and emulsions.
Jorgensen
Lene
L
Department of Pharmaceutics and Analytical Chemistry, The Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100-Copenhagen, Denmark.
Martins
Susana
S
van de Weert
Marco
M
D003460:Crystallization / Q000379:methods*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D004795:Enzyme Stability; D008055:Lipids / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D009113:Muramidase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D013499:Surface Properties
D004337:Drug Carriers; D008055:Lipids; D046911:Macromolecular Substances; D009113:Muramidase