title: Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli.
abstract: Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xinmofan Road #5, Nanjing 210009, China. bioengine@njut.edu.cn
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Liu
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Ke-Quan
authors.initials: KQ
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Yue
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006038:Glyoxylates; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D019802:Succinic Acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose