A new multiple-drug applicator with minimal drug cross-talk, leakage, and consumption.
The relative effects of multiple drugs give an important clue to dissect a neuronal mechanism and to seek for a candidate neurotherapeutical agent. Here we have devised a "flute" applicator which can deliver several drugs to a neural cell preparation. The applicator stands by, cleaning itself with bath perfusate and delivers drugs only during test applications. This minimizes drug cross-talk in and leakage from the applicator and drug consumption. Using the applicator, we successfully compared the relative effects of widely different doses of an agonist in single neurons. The flute applicator would be a useful tool for pharmacological analyses.
Fujita
Yosuke
Y
Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
Shimomura
Takeshi
T
Hosoguchi
Masafumi
M
Kano
Masanobu
M
Fukurotani
Kenkichi
K
Tabata
Toshihide
T
D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004801:Eosine Yellowish-(YS) / Q000008:administration & dosage; D004868:Equipment Failure; D005456:Fluorescent Dyes / Q000008:administration & dosage; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000008:administration & dosage; D051379:Mice; D018408:Patch-Clamp Techniques; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D010600:Pharmacology; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists; D012118:Resorcinols / Q000008:administration & dosage / Q000494:pharmacology
D005456:Fluorescent Dyes; D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D018094:Receptors, Metabotropic Glutamate; D012118:Resorcinols; C104077:metabotropic glutamate receptor type 1; C079215:3,5-dihydroxyphenylglycine; C017475:lucifer yellow; D004801:Eosine Yellowish-(YS); D005998:Glycine