[A prospective study of the relationship between serum hepatitis B virus DNA and the risk of primary liver cancer].
OBJECTIVE
To determine the relationship between the serum hepatitis B virus (HBV) DNA and the risk of primary liver cancer (PLC).


METHODS
Farmers aged 30 to 55 years in Long An county were recruited in this study Blood samples were collected and the sera were tested for HBsAg using Enzyme-Linked ImmunoSorbent Assay (ELISA), and the HBsAg-positive sera were further tested for viral DNA using nested polymerase chain reaction (nPCR). The study subjects were divided into three groups. The first group was positive for both HBsAg and HBV DNA. The second group was positive for HBsAg but negative for HBV DNA. Age-, sex-, residence-matched HBsAg negative controls for group 1 and group 2 were enrolled in the third group. The cohort was followed up for four years.


RESULTS
The positive rate of HBsAg in these farmers was 14.52% (3975/27,379), and the HBV DNA positive rate in HBsAg positive subjects was 40.35% (1604/3975). The total PLC incidence rate in Group 1 and 2 was 672.45 /100,000 person-years (PY), significantly higher than that in Group3 (17.19 /100,000 PY). The relative risk (RR) was 39.123, and the 95% confidence interval (CI) was 9.018-159.146. The PLC incidence rate of Group 1 (984.03/100,000 PY) was significantly higher than that of Group2 (324.38 /100,000 PY). The RR was 3.034, and the 95% CI was 1.795-5.125. Multivariate analyses of Group1 and 2 with Cox model showed that sex, age, serum HBV DNA, and family history of PLC were independent risk factors of PLC.


CONCLUSIONS
HBV DNA and HBsAg positive subjects have a higher chance to develop PLC than HBV DNA negative-, HBsAg positive subjects.
Chen
Qin-yan
QY
Division of Viral Disease Prevention and Control, Guangxi Zhuang Autonmous Region Center For Disease Prevention and Control, Nanning 530028, China.
Dong
Bai-qing
BQ
Yang
Jin-ye
JY
Wei
Shao-chao
SC
Fang
Kong-xiong
KX
Wang
Xue-yan
XY
Fang
Zhong-liao
ZL
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000821:virology*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006509:Hepatitis B / Q000150:complications*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000453:epidemiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012307:Risk Factors; D019562:Viral Load; D055815:Young Adult
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens