title: Reliable detection of dead microbial cells by using fluorescent hydrazides.
abstract: We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.
authors.affiliation: INSERM U571, Faculté de Médecine Paris Descartes, Université René Descartes, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Saint-Ruf
authors.forename: Corinne
authors.initials: C
authors.lastname: Cordier
authors.forename: Jérome
authors.initials: J
authors.lastname: Mégret
authors.forename: Ivan
authors.initials: I
authors.lastname: Matic
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D050296:Microbial Viability; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes