[Metabonomic study on the effects of allicin on rats].
To investigate the effects of allicin on rats by NMR-based metabonomic method, the changes of endogenous metabolites in normal rat urine and the influences on metabolism were analyzed with bio-nuclear magnetic resonance (NMR) method and partial least-squares discriminant analysis (PLS-DA) after intraperitoneal administration of allicin solution. The identified biochemical effects associated with allicin dosing included elevated then gradually recovered urinary levels of Kreb's cycle intermediates, such as citrate, alpha-ketoglutarate and succinate and increased concentrations of ketones. Meanwhile, decreased urinary concentrations of glucose, lactate, alanine, hippurate and trimethylamine oxide were observed. The PLS-DA revealed that the metabonomic profiles of allicin treated groups were obviously different from those of the control group. Allicin may change metabolism significantly in normal rats. The study of the pharmacologic mechanism of allicin by metabonomic method is practicable and it could be explored further.
Wang
Li
L
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Song
Min
M
Hang
Tai-Jun
TJ
Zhang
Zheng-Xing
ZX
Shen
Wen-Bin
WB
Song
Zhe
Z
Chen
Jian
J
D000818:Animals; D004220:Disulfides; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013441:Sulfinic Acids / Q000378:metabolism* / Q000652:urine*
D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin