CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells.
Recent studies have challenged the view that Langerhans cells (LCs) constitute the exclusive antigen-presenting cells of the skin and suggest that the dermal dendritic cell (DDC) network is exceedingly complex. Using knockin mice to track and ablate DCs expressing langerin (CD207), we discovered that the dermis contains five distinct DC subsets and identified their migratory counterparts in draining lymph nodes. Based on this refined classification, we demonstrated that the quantitatively minor CD207+ CD103+ DDC subset is endowed with the unique capability of cross-presenting antigens expressed by keratinocytes irrespective of the presence of LCs. We further showed that Y-Ae, an antibody that is widely used to monitor the formation of complexes involving I-Ab molecules and a peptide derived from the I-E alpha chain, recognizes mature skin DCs that express I-Ab molecules in the absence of I-E alpha. Knowledge of this extra reactivity is important because it could be, and already has been, mistakenly interpreted to support the view that antigen transfer can occur between LCs and DDCs. Collectively, these data revisit the transfer of antigen that occurs between keratinocytes and the five distinguishable skin DC subsets and stress the high degree of functional specialization that exists among them.
Henri
Sandrine
S
Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.
Poulin
Lionel Franz
LF
Tamoutounour
Samira
S
Ardouin
Laurence
L
Guilliams
Martin
M
de Bovis
Béatrice
B
Devilard
Elisabeth
E
Viret
Christophe
C
Azukizawa
Hiroaki
H
Kissenpfennig
Adrien
A
Malissen
Bernard
B
D000818:Animals; D017951:Antigen Presentation / Q000502:physiology*; D000941:Antigens / Q000235:genetics / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D020405:Dermis / Q000166:cytology / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology / Q000502:physiology; D055879:Gene Knock-In Techniques; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D039001:Integrin alpha Chains / Q000235:genetics / Q000276:immunology*; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology*; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology*; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity
D000941:Antigens; D015703:Antigens, CD; D000954:Antigens, Surface; C498497:Cd207 protein, mouse; D000949:Histocompatibility Antigens Class II; C474482:I-A(b) antigen, mouse; C024138:I-E-antigen; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; C091367:alpha E integrins