Cu2+-labeled, SPION loaded porous silica nanoparticles for cell labeling and multifunctional imaging probes.
We have developed an ion-sensing nanoparticle that is comprised of a superparamagnetic iron oxide (SPIO) core encapsulated with a porous silica shell. The latter can be readily anchored with ligands capable of coordinating with positron-emitting metal. Evidently, this nanoparticle has a great potential for use in cell tracking with magnetic resonance (MR) imaging and positron emission tomography (PET). Herein we report the synthesis, surface functionalization and characterization of the magnetic nanoparticle-based probes and evaluate their cell-labeling efficacy, cytotoxicity and relaxivity in comparison to one of the most commonly utilized MRI contrast agents, Feridex.
Patel
Daksha
D
Steacie Institute for Molecular Science, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R6, Canada. daksha.patel@nrc-cnrc.gc.ca
Kell
Arnold
A
Simard
Benoit
B
Deng
Jixian
J
Xiang
Bo
B
Lin
Hung-Yu
HY
Gruwel
Marco
M
Tian
Ganghong
G
D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D003300:Copper / Q000378:metabolism*; D018360:Crystallography, X-Ray; D003911:Dextrans; D052203:Ferrosoferric Oxide / Q000737:chemistry / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D058185:Magnetite Nanoparticles; D008297:Male; D008855:Microscopy, Electron, Scanning; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D019047:Phantoms, Imaging; D051381:Rats; D011917:Rats, Inbred Lew; D012822:Silicon Dioxide / Q000737:chemistry*; D013194:Staining and Labeling
D003911:Dextrans; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D012822:Silicon Dioxide; D003300:Copper; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide