title: Gene expression profile of acupuncture treatment in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model.
abstract: OBJECTIVE
  To find new biomarkers by stimulating acupuncture point GB34 (Yangneungcheon) which has neuroprotective effect on the mouse model of Parkinson's disease, analysis of cDNA microarray on mRNAs of the substantia nigra was performed.
  
  
  METHODS
  Male C57BL/6 mice were divided into two groups: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice (MPTP group, n=3); 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and acupuncture (GB34)-treated mice (MPTP + ACU group, n=3). The mice received an intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (30 mg/kg) once daily for 3 consecutive days. Manual acupuncture was performed 2 hours after every injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The total RNA in the substantia nigra of each mouse was isolated on 3 days after the last 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection. Agilent mouse whole genome 44K chip was used for microarray analysis and the hybridization image was analysed by GenePix Pro 6.0. Data normalization and analysis were performed using GeneSpring GX 7.3.1 program.
  
  
  RESULTS
  The acupuncture stimulation revealed 799 genes (424 up- and 375 down-regulated) of which expression levels were changed more than two-folds in the MPTP + ACU group, compared to the MPTP group. The genes selected were classified into several categories based on their functions using DAVID Bioinformatics Resources 2008 (http://david.abcc.ncifcrf.gov/) and KEGG PATHWAY Database (http://www.genome.jp/kegg/pathway.html).
  
  
  CONCLUSIONS
  Biomarkers in response to acupuncture stimulation to GB34 were identified in a mouse model for Parkinson's disease. These biomarkers might provide a promising clue for understanding the neuroprotective effect of acupuncture in Parkinson's disease.
authors.affiliation: Kohwang Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Mee Sook
authors.initials: MS
authors.lastname: Hong
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Yang
authors.forename: Hi-Joon
authors.initials: HJ
authors.lastname: Park
authors.forename: Seung-Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Seung-Nam
authors.initials: SN
authors.lastname: Kim
authors.forename: Ji-Yeun
authors.initials: JY
authors.lastname: Park
authors.forename: Jeong Yoon
authors.initials: JY
authors.lastname: Song
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Dae Jean
authors.initials: DJ
authors.lastname: Jo
authors.forename: Sung Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Dong
authors.initials: D
authors.lastname: HwanYun
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Ban
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015670:Acupuncture Therapy; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D030541:Databases, Genetic; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D020407:Internet; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D012984:Software; D013378:Substantia Nigra / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine