title: The effect of mosapride (5HT-4 receptor agonist) on insulin sensitivity and GLUT4 translocation.
abstract: OBJECTIVE
  We investigated the effect of mosapride, 5HT-4 (5-hydroxytryptamine) agonist, on blood glucose level and insulin sensitivity in subjects with impaired glucose tolerance (IGT) and conducted an in vitro study to evaluate the action mechanism.
  
  
  METHODS
  Thirty IGT patients were randomly assigned to receive either mosapride or placebo for 2 weeks. Biochemical profiles and insulin sensitivity index from euglycemic hyperinsulinemic clamp test were assessed before and after treatment. In cultured myotubes from human skeletal muscle cells, insulin- and mosapride-induced GLUT4 translocation and tyrosine phosphorylation of IRS-1 were determined.
  
  
  RESULTS
  After 2 weeks of treatment with mosapride, glucose disposal rates were significantly increased up to those of control (mosapride 5.47+/-1.72 vs 7.06+/-2.13, P=0.004, placebo 5.42+/-1.85 vs 5.23+/-1.53mgkg(-1)min(-1)). Fasting plasma glucose (FPG) and insulin levels were decreased. Mosapride increased the contents of GLUT4 in plasma membrane representing the increased recruitment of glucose transporters from intracellular pool. While insulin treatment on human skeletal muscle cell resulted in an increased tyrosine phosphorylation of IRS-1, mosapride did not have any effect.
  
  
  CONCLUSIONS
  Mosapride is effective in decreasing FPG without stimulating insulin secretion in IGT subjects, possibly by inducing GLUT4 translocation in skeletal muscles.
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Nam
authors.forename: J Y
authors.initials: JY
authors.lastname: Nam
authors.forename: J S
authors.initials: JS
authors.lastname: Yoo
authors.forename: M
authors.initials: M
authors.lastname: Cho
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: C W
authors.initials: CW
authors.lastname: Ahn
authors.forename: B S
authors.initials: BS
authors.lastname: Cha
authors.forename: E J
authors.initials: EJ
authors.lastname: Lee
authors.forename: S K
authors.initials: SK
authors.lastname: Lim
authors.forename: K R
authors.initials: KR
authors.lastname: Kim
authors.forename: H C
authors.initials: HC
authors.lastname: Lee
mesh_terms: D000328:Adult; D001549:Benzamides / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D015309:Glucose Clamp Technique; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009025:Morpholines / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D016037:Single-Blind Method
chemical_list: D001549:Benzamides; D001786:Blood Glucose; D051275:Glucose Transporter Type 4; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D008055:Lipids; D009025:Morpholines; C062720:mosapride