title: [Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene].
abstract: OBJECTIVE
  To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.
  
  
  METHODS
  A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.
  
  
  RESULTS
  Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.
  
  
  CONCLUSIONS
  BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
authors.affiliation: Center Laboratory, Fourth Medical Military University, Xi'an 710032, China. yinwen@fmmu.edu.cn
authors.forename: Wen
authors.initials: W
authors.lastname: Yin
authors.forename: Xin
authors.initials: X
authors.lastname: Lü
authors.forename: Ying-feng
authors.initials: YF
authors.lastname: Lei
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Meng-ning
authors.initials: MN
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Hai-yun
authors.initials: HY
authors.lastname: Lan
authors.forename: Ai-qin
authors.initials: AQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000097:blood; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C050806:presurface protein 1, hepatitis B surface antigen