title: Crystallization and preliminary X-ray diffraction data analysis of stenodactylin, a highly toxic type 2 ribosome-inactivating protein from Adenia stenodactyla.
abstract: Ribosome-inactivating proteins (RIPs) inhibit protein synthesis and induce cell death by removing a single adenine from a specific rRNA loop. They can be divided into two main groups: type 1 and type 2 RIPs. Type 1 RIPs are single-chain enzymes with N-glycosidase activity. Type 2 RIPs contain two chains (A and B) linked by a disulfide bond. The A chain has RIP enzymatic activity, whereas the B chain shows lectin activity and is able to bind to glycosylated receptors on the cell surface. Stenodactylin is a type 2 RIP from the caudex of Adenia stenodactyla from the Passifloraceae family that has been recently purified and characterized. It shows a strong enzymatic activity towards several substrates and is more cytotoxic than other toxins of the same type. Here, the crystallization and preliminary X-ray diffraction data analysis of stenodactylin are reported. This RIP forms crystals that diffract to high resolution (up to 2.15 A). The best data set was obtained by merging data collected from two crystals. Stenodactylin crystals belonged to the centred monoclinic space group C2 and contained two molecules in the asymmetric unit.
authors.affiliation: Department of Chemistry G. Ciamician', Via Selmi 2, Alma Mater Studiorum University of Bologna, I-40126 Bologna, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Tosi
authors.forename: Simona
authors.initials: S
authors.lastname: Fermani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Falini
authors.forename: Letizia
authors.initials: L
authors.lastname: Polito
authors.forename: Massimo
authors.initials: M
authors.lastname: Bortolotti
authors.forename: Andrea
authors.initials: A
authors.lastname: Bolognesi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D037102:Lectins / Q000737:chemistry*; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D054790:Ribosome Inactivating Proteins, Type 2
chemical_list: D037102:Lectins; D054790:Ribosome Inactivating Proteins, Type 2; D009699:N-Glycosyl Hydrolases; C523578:stenodactylin protein, Adenia stenodactyla