Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni.
Lyophilization was used to concentrate bovine feces prior to DNA extraction and analysis using real-time PCR. Lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both Escherichia coli O157:H7 and Campylobacter jejuni bacteria present in feces at concentrations ranging between 2 log(10) and 6 log(10) CFU g(-)(1).
Rapp
Delphine
D
Climate, Land and Environment, AgResearch, East Street, Private Bag 3123, Hamilton 3240, New Zealand. Delphine.Rapp@agresearch.co.nz
Waller
John
J
Brightwell
Gale
G
Muirhead
Richard W
RW
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005612:Freeze Drying; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
D004269:DNA, Bacterial