title: Cellular activity of bioactive nanograined/ultrafine-grained materials.
abstract: Our recent electron microscopy study on biomimetic nanostructured coatings on nanograined/ultrafine-grained (NG/UFG) substrates [Mater Sci Eng C 2009;29:2417-27] indicated that electrocrystallized nanohydroxyapatite (nHA) on phase-reversion-induced NG/UFG substrates exhibited a vein-type interconnected and fibrillar structure that closely mimicked the hierarchical structure of bone. The fibrillar structure on NG/UFG substrate is expected to be more favorable for cellular response than a planar surface. In contrast, hydroxyapatite (HA) coating on coarse-grained (CG) substrate more closely resembled a film rather than a fibrillar structure. Inspired by the differences in the structure of HA coating, we describe here the cell-substrate interactions of pre-osteoblasts (MC 3T3-E1) on bioactive NG/UFG and CG austenitic stainless steel substrates. NG/UFG austenitic stainless steel was obtained by a novel controlled phase-reversion annealing of cold-deformed austenite. This example provides an illustration of how a combination of cellular and molecular biology, materials science and engineering can advance our understanding of cell-substrate interactions. Interestingly, the cellular response of nanohydroxyapatite (nHA)-coated NG/UFG substrate demonstrated superior cytocompatibility, improved initial cell attachment, higher viability and proliferation, and well-spread morphology in relation to HA-coated CG substrate and their respective uncoated (bare) counterparts as implied by fluorescence and electron microscopy and MTT assay. Similar conclusions were derived from an immunofluorescence study that involved examination of the expression levels of vinculin focal adhesion contacts associated with dense actin stress fibers and fibronectin, protein analysis through protein bands in SDS-PAGE, and quantitative total protein assay. The enhancement of cellular response followed the sequence: nHA-coated NG/UFG>nHA-coated CG>NG/UFG>CG substrates. The outcomes of the study are expected to counter the challenges associated with the engineering of nanostructured surfaces with specific physical and surface properties for medical devices with significantly improved cellular response.
authors.affiliation: Center for Structural and Functional Materials, University of Louisiana at Lafayette, Lafayette, LA 70504, USA. dmisra@louisiana.edu
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
authors.forename: W W
authors.initials: WW
authors.lastname: Thein-Han
authors.forename: S A
authors.initials: SA
authors.lastname: Mali
authors.forename: M C
authors.initials: MC
authors.lastname: Somani
authors.forename: L P
authors.initials: LP
authors.lastname: Karjalainen
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008856:Microscopy, Fluorescence; D049329:Nanostructures
chemical_list: D017886:Durapatite