Discrepancy in dengue virus neutralizing antibody titers between plaque reduction neutralizing tests with Fcgamma receptor (FcgammaR)-negative and FcgammaR-expressing BHK-21 cells.
Protective immunity against dengue virus (DENV) is best reflected by the presence of neutralizing antibodies. The conventional plaque reduction neutralizing test (PRNT) is performed using Fcgamma receptor (FcgammaR)-negative cells. Because FcgammaR plays a key role in antibody-dependent enhancement, we examined neutralizing antibody titers of mouse monoclonal antibodies and human serum samples in PRNTs using FcgammaRIIA-negative and FcgammaRIIA-expressing BHK cells. There was a discrepancy in dengue virus neutralizing antibody titers between PRNTs using FcgammaRIIA-negative versus FcgammaRIIA-expressing BHK cells. Neutralizing antibody titers to DENV-1 and DENV-2 tested with monoclonal antibodies, and with most of the human serum samples, were higher in assays using BHK cells than those using FcgammaRIIA-expressing BHK cells. The results suggest that neutralizing antibody titers determined using FcgammaRIIA-expressing cells may better reflect the protective capacity of anti-DENV antibodies, as the major target cells of DENV infection are FcgammaR-positive cells.
Moi
Meng Ling
ML
Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. kurane@nih.go.jp
Lim
Chang-Kweng
CK
Kotaki
Akira
A
Takasaki
Tomohiko
T
Kurane
Ichiro
I
D000818:Animals; D057134:Antibodies, Neutralizing / Q000032:analysis / Q000097:blood*; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood*; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D017452:Receptors, IgG / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C080568:Fc gamma receptor IIA; D017452:Receptors, IgG