[Effects of water stress on protein expression and physiological properties of different genotype wheat (Triticum aestivum L.) sprouts].
With drought-resistant wheat (Triticum aestivum) cultivar Changwu 134 and drought-sensitive cultivar Zhengyin 1 as test materials, and by using -1.2 MPa PEG 6000 to treat their seeds, this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions. SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39.5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts. Under normal water supply, the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth. This protein was preliminarily named as water-sensitive protein, due to its water-sensitivity and of newly discovered protein. The determinations of physiological properties showed that under water stress, the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content, but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.
Dai
Ming
M
College of Life Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China. daiming5014@163.com
Deng
Xi-ping
XP
Yang
Shu-shen
SS
Cao
Rang
R
Guo
Hong-bo
HB
Zhang
Fang
F
D005838:Genotype; D010940:Plant Proteins / Q000378:metabolism*; D036226:Seedlings / Q000737:chemistry / Q000502:physiology; D013312:Stress, Physiological; D014908:Triticum / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014867:Water / Q000378:metabolism
D010940:Plant Proteins; D014867:Water