Ligand trapping by cytochrome c oxidase: implications for gating at the catalytic center.
Cytochrome c oxidase is a member of the heme-copper family of oxygen reductases in which electron transfer is linked to the pumping of protons across the membrane. Neither the redox center(s) associated with proton pumping nor the pumping mechanism presumably common to all heme-copper oxidases has been established. A possible conformational coupling between the catalytic center (Fe(a3)(3+)-Cu(B)(2+)) and a protein site has been identified earlier from ligand binding studies, whereas a structural change initiated by azide binding to the protein has been proposed to facilitate the access of cyanide to the catalytic center of the oxidized bovine enzyme. Here we show that cytochrome oxidase pretreated with a low concentration of azide exhibits a significant increase in the apparent rate of cyanide binding relative to that of free enzyme. However, this increase in rate does not reflect a conformational change enhancing the rapid formation of a Fe(a3)(3+)-CN-Cu(B)(2+) complex. Instead the cyanide-induced transition of a preformed Fe(a3)(3+)-N(3)-Cu(B)(2+) to the ternary complex of Fe(a3)(3+)-N(3) Cu(B)(2+)-CN is the most likely reason for the observed acceleration. Significantly, the slow rate of azide release from the ternary complex indicates that cyanide ligated to Cu(B) blocks a channel between the catalytic site and the solvent. The results suggest that there is a pathway that originates at Cu(B) and that, during catalysis, ligands present at this copper center control access to the iron of heme a(3) from the bulk medium.
Parul
Dzmitry
D
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
Palmer
Graham
G
Fabian
Marian
M
D000818:Animals; D001386:Azides / Q000378:metabolism; D002384:Catalysis; D002417:Cattle; D003486:Cyanides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006418:Heme / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D010084:Oxidation-Reduction; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared
D001386:Azides; D003486:Cyanides; D008024:Ligands; D006418:Heme; D003576:Electron Transport Complex IV