Characterization of the human neutrophil alloantigen-3a.
Transfusion-related acute lung injury (TRALI) is a frequent cause of transfusion-associated morbidity and mortality. Severe TRALI is often due to antibodies in blood components directed against the human neutrophil alloantigen-3a (HNA-3a). We show here that the HNA-3a antigen arises from a nucleotide polymorphism in the choline transporter-like protein-2 gene (SLC44A2), with the resulting variation at amino acid position 154 determining the reactivity of the protein with HNA-3a-specific antibodies; the variant with an arginine at this position, rather than a glutamine, constitutes the HNA-3a antigen. The molecular identification of this antigen should facilitate the development of assays for blood donor screening to lower the risk of TRALI.
Greinacher
Andreas
A
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz-Arndt-Universität Greifswald, Greifswald, Germany. greinach@uni-greifswald.de
Wesche
Jan
J
Hammer
Elke
E
Fürll
Birgitt
B
Völker
Uwe
U
Reil
Angelika
A
Bux
Jürgen
J
D055371:Acute Lung Injury / Q000235:genetics* / Q000276:immunology; D000372:Agglutination Tests; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D065227:Transfusion Reaction
C518134:HNA-3a antigen, human; D007519:Isoantigens; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C484524:SLC44A2 protein, human