title: [Real-time PCR in rapid diagnosis of Aeromonas hydrophila necrotizing soft tissue infections].
abstract: We report a case of rapidly progressive necrotizing soft tissue infection and sepsis followed by a patient's death. We suspected Vibrio vulnificus infection because the patient's underlying disease was cirrhosis and the course extremely rapid. No microbe had been detected at death. We extracted DNA from a blood culture bottle. SYBR green I real-time PCR was conducted but could not detect V. vulnificus vvh in the DNA sample. Aeromonas hydrophila was cultured and identified in blood and necrotized tissue samples. Real-time PCR was conducted to detect A. hydrophila ahh1, AHCYTOEN and aerA in the DNA sample extracted from the blood culture bottle and an isolated necrotized tissue strain, but only ahh1 was positive. High-mortality in necrotizing soft tissue infections makes it is crucial to quickly detect V. vulnificus and A. hydrophila. We found real-time PCR for vvh, ahh1, AHCYTOEN, and aerA useful in detecting V. vulnificus and A. hydrophila in necrotizing soft tissue infections.
authors.affiliation: Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kohayagawa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Izumi
authors.forename: Misuzu
authors.initials: M
authors.lastname: Ushita
authors.forename: Norio
authors.initials: N
authors.lastname: Niinou
authors.forename: Masayuki
authors.initials: M
authors.lastname: Koshizaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamori
authors.forename: Sakae
authors.initials: S
authors.lastname: Kaneko
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fukushima
mesh_terms: D016980:Aeromonas hydrophila; D000368:Aged; D003199:Computer Systems; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009336:Necrosis; D016133:Polymerase Chain Reaction; D018461:Soft Tissue Infections / Q000175:diagnosis*