Proliferation and apoptotic rates and increased frequency of p63-positive cells in the prostate acinar epithelium of alloxan-induced diabetic rats.
The effects of experimental type 1 diabetes were investigated in the acinar epithelium of rat ventral prostate, focusing on the rates of cell proliferation and the frequency of apoptosis and p63-positive cells. Type 1 diabetes was induced in adult male Wistar rats by a single alloxan administration (42 mg/kg b.w.) and its effects were analysed for 1 week and 3 months after the establishment of the disease. A group of diabetic rats was treated daily with 5 IU of insulin during 1 week after diabetes had been diagnosed. Immunocytochemical methods for the localization of cell proliferation antigen (PCNA), androgen receptor (AR) and p63 protein were carried out, and apoptotic cells were identified by TUNEL essay. In diabetic rats, testosterone levels reduced drastically after 1 week and in a lower degree after 3 months. In short-term diabetic rats, cell proliferation decreased, and in medium-term, epithelial apoptotic rates increased. In both periods after the onset of diabetes, the frequency of p63-positive cells doubled. Insulin treatment was effective in preventing testosterone decrease, p63-positive cell increase and apoptotic rates, but did not interfere in cell proliferation. This investigation shows that, soon after diabetes onset, there are important modifications in cell proliferation within the acinar prostatic epithelium, and in longer term, there is a marked impact on kinetics of differentiation and cell death, which may initially be attributable to an androgenic fall, but is probably also because of other factors related to diabetes, as changes are considerably different from those resulting from castration.
Arcolino
Fanny Oliveira
FO
Department of Biology, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
Ribeiro
Daniele Lisboa
DL
Gobbo
Marina Guimarães
MG
Taboga
Sebastião Roberto
SR
Góes
Rejane Maira
RM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001786:Blood Glucose / Q000096:biosynthesis; D001835:Body Weight; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D012739:Gonadal Steroid Hormones / Q000097:blood; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D001786:Blood Glucose; D012739:Gonadal Steroid Hormones; D007328:Insulin; D018809:Proliferating Cell Nuclear Antigen; D011944:Receptors, Androgen; C108581:Tp63 protein, rat; D016159:Tumor Suppressor Protein p53