[Inhibitory effect of dexamethasone on myeloid differentiation factor 88 and tumor necrosis factor-alpha expressions in mouse peritoneal macrophages].
OBJECTIVE
To study the inhibitory effect of dexamethasone (DEX) on myeloid differentiation factor 88 (MyD88) and tumor necrosis factor-alpha (TNF-alpha) expression in mouse peritoneal macrophages in innate immune response to Penicillium marneffei (PM).


METHODS
Mouse peritoneal macrophages were cultured in the presence of heat-inactivated yeast-phase PM with or without DEX, and the protein and mRNA expressions of MyD88 in the macrophages were detected using Western blotting and real-time PCR, respectively. TNF-alpha in the cell culture supernatant was measured with enzyme-linked immunosorbent assay.


RESULTS
DEX suppressed TNF-alpha production by the macrophages co-cultured with PM. The expressions of MyD88 were up-regulated by PM stimulation, whose effect was inhibited by the application of DEX.


CONCLUSIONS
The inhibitory effect of DEX on PM-induced proinflammatory responses of the macrophage is directly associated with the inhibition of MyD88 expression.
Zhao
Wen-jie
WJ
Department of Dermatology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. zhaowenjie1972@sohu.com
Xi
Li-yan
LY
Ma
Li
L
Zhang
Jun-min
JM
Li
Xi-qing
XQ
Lu
Chang-ming
CM
Li
Fei
F
D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053594:Myeloid Differentiation Factor 88 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010407:Penicillium / Q000254:growth & development; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D053594:Myeloid Differentiation Factor 88; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone