[Inhibitory effect of ginseng saponin IH901 on proliferation and metastasis of ECV304 cell line and its molecular mechanism].
This study aims to investigate the inhibitory effect on proliferation and metastasis of 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol (IH901) on ECV304 cell line. MTT assay was used to examine the effect of cell proliferation inhibition and the adhesive ability of ECV304 cells to artificial basement membrane. Morphology of cell apoptosis was observed with phase contrast microscope. Apoptosis rate and cell cycle were detected by flow cytometry (FCM). Cell migration was measured by wound healing assay. ELISA kit was used to detect VEGF and bFGF. Caspases were detected by Western blotting. Results indicated that ginseng saponin IH901 can downregulate the expression of growth promoting protein VEGF and bFGF, and upregulate pro apoptosis protein cleaved caspase-9 and cleaved caspase-3. The increase in the apoptotic sub-G1 fraction is in a dose-dependent manner, and cell cycle arrests in the G0/G1 phase was detected by FCM. Morphological examination of IH901-treated samples showed cells with chromatin condensation, cell shrinkage, and all typical characteristics of apoptotic cells. Therefore, IH901 dramatically suppresses cell proliferation and adhesion and migration of ECV304 cell line.
Ming
Yan-Lin
YL
The Research and Development Center for Medicine Plant and Plant Drugs, Xiamen Oversea Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China. xmyanlin@yahoo.com.cn
Chen
Zhong-Yan
ZY
Chen
Liang-Hua
LH
Tong
Qing-Xuan
QX
Zheng
Zhi-Zhong
ZZ
Zheng
Guo-Hua
GH
Qi
Xiao-Hui
XH
D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D005894:Panax; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology
C482402:20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol; D012502:Sapogenins; D012503:Saponins