[Physico-chemical investigation of blood components using special fluorescent probes].
The objective of this paper was to overview results of the works aimed at the development and practical use of fluorescent dies (molecular flourescent probes) for the study of blood components carried out at the Institute of Physico-Chemical Medicine under the guidance of prof. Yu.M. Lopukhin. The new probes were used to directly detect and measure lipoproteins in unfractionated plasma and serum for the identification of risk factors of cardiovascular diseases. The study revealed changes in physico-chemical characteristics of albumin molecules in many pathological processes closely related to clinical features and severity of the disease. These data were used to develop a simple albumin test for clinical application. Probes for measuring electric field potential in leukocytes make it possible to diagnose changes of this parameter in patients with immune disorders. It is concluded that they carry diagnostically valuable information and may be helpful for recording pathological changes at the cellular and molecular levels that can not be observed by traditional laboratory methods.
Dobretsov
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D001774:Blood Chemical Analysis / Q000379:methods*; D002318:Cardiovascular Diseases / Q000097:blood* / Q000175:diagnosis; D005456:Fluorescent Dyes; D006801:Humans; D008055:Lipids / Q000097:blood*
D005456:Fluorescent Dyes; D008055:Lipids