Proteolipid protein 2 is associated with melanoma metastasis.
The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.
Sonoda
Yoshiko
Y
Faculty of Pharmacy, Keio University, Tokyo, Japan. sonoda-ys@pha.keio.ac.jp
Warita
Masako
M
Suzuki
Takaharu
T
Ozawa
Hiroki
H
Fukuda
Yusuke
Y
Funakoshi-Tago
Megumi
M
Kasahara
Tadashi
T
D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary; D062792:MARVEL Domain-Containing Proteins; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008546:Melanoma, Experimental / Q000235:genetics* / Q000473:pathology*; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009362:Neoplasm Metastasis; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011510:Proteolipids; D011817:Rabbits; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured
D062792:MARVEL Domain-Containing Proteins; D008565:Membrane Proteins; D018991:Myelin Proteolipid Protein; C435750:Plp2 protein, mouse; D011510:Proteolipids; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D020778:Matrix Metalloproteinase 2