[HIV-1 Nef regulates ICAM-1 expression on endothelial cells via Erk /Mapk signaling pathway].
OBJECTIVE
To explore the effect of HIV-1 Nef on the ICAM-1 expression of endothelial cells ECV-304 and its signaling pathways.


METHODS
ICAM-1 expression on the endothelial cells ECV-304 was detected by Western blot and FCM assay. Kinase inhibitor PD98059 was used in the cells to analyze the ERK signaling pathway.


RESULTS
Western blot showed that ICAM-1 and p-ERK protein expression increased in Nef expressed ECV304 cells (ECV304-Nef) and FCM results showed that the percentage of ICAM-1 positive cells in ECV304-Nef and control cells was (35.3+/-2.2)% and (12.5+/-0.8)% respectively (P>0.01). p-ERK inhibitor PD98059 almost completely blocked the Nef up-regulation of the p-ERK and ICAM-1. When p-ERK inhibitor was added, the percentage of ICAM-1 positive cells in ECV304-Nef (11.4+/-1.1)% was reduced to the level of the control cells (10.4+/-1.5)% (P>0.05).


CONCLUSIONS
Erk/Mapk signaling pathway may contribute to the over-expression of adhesion molecules ICAM-1 gene in HIV-1 Nef positive cells. These findings may provide the basis for further research on the mechanism and treatment of HIV-1 infection.
Yang
Fan
F
Department of Internal Medicine, Second People's Hospital of Yichang, Yichang 443000, China. yangf823@162.com
Liu
Chao-qi
CQ
Qin
Xiao-lin
XL
Shi
Ji-jing
JJ
D042783:Endothelial Cells / Q000201:enzymology / Q000378:metabolism* / Q000821:virology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1; D018799:Intercellular Adhesion Molecule-1; D048049:Extracellular Signal-Regulated MAP Kinases