Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor D.
The complement system is an essential component of innate immunity, participating in the pathogenesis of inflammatory diseases and in host defense. In the lectin complement pathway, mannose-binding lectin (MBL) and ficolins act as recognition molecules, and MBL-associated serine protease (MASP) is a key enzyme; MASP-2 is responsible for the lectin pathway activation. The function of other serine proteases (MASP-1 and MASP-3) is still obscure. In this study, we generated a MASP-1- and MASP-3-deficient mouse model (Masp1/3-/-) and found that no activation of the alternative pathway was observed in Masp1/3-/- serum. Mass spectrometric analysis revealed that circulating complement factor D (Df) in Masp1/3-/- mice is a zymogen (pro-Df) with the activation peptide QPRGR at its N terminus. These results suggested that Masp1/3-/- mice failed to convert pro-Df to its active form, whereas it was generally accepted that the activation peptide of pro-Df is removed during its secretion and factor D constitutively exists in an active form in the circulation. Furthermore, recombinant MASP-1 converted pro-Df to the active form in vitro, although the activation mechanism of pro-Df by MASP-1 is still unclear. Thus, it is clear that MASP-1 is an essential protease of both the lectin and alternative complement pathways.
Takahashi
Minoru
M
Department of Immunology, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. minolta@fmu.ac.jp
Ishida
Yumi
Y
Iwaki
Daisuke
D
Kanno
Kazuko
K
Suzuki
Toshiyuki
T
Endo
Yuichi
Y
Homma
Yoshimi
Y
Fujita
Teizo
T
D041721:3T3-L1 Cells; D000818:Animals; D011416:Complement Factor D / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003170:Complement Pathway, Alternative / Q000502:physiology*; D037582:Complement Pathway, Mannose-Binding Lectin / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology; D037102:Lectins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D000097604:Ficolins
D011416:Complement Factor D; D037102:Lectins; D037601:Mannose-Binding Lectin; D050606:Mannose-Binding Protein-Associated Serine Proteases; D000097604:Ficolins