[Progress of study on the detection technique of microRNA].
MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. MiRNAs are involved in critical biologic processes, including development, cell differentiation, proliferation and the pathogenesis of disease. This review focuses on recent researches on the detection techniques of miRNA including micorarray technique, Northern blot, real-time quantitative PCR, detection technique of miRNA function and so on.
Zhao
Hai-Feng
HF
National Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Peking Union Medical College & Chinese Academy of Medical Sciences, Tianjing 300020, China.
Yang
Ren-Chi
RC
D015152:Blotting, Northern; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification*; D046208:Microchip Analytical Procedures; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
D035683:MicroRNAs