title: Whole-genome scan identifies quantitative trait loci for chronic pastern dermatitis in German draft horses.
abstract: Chronic pastern dermatitis (CPD), also known as chronic progressive lymphedema (CPL), is a skin disease that affects draft horses. This disease causes painful lower-leg swelling, nodule formation, and skin ulceration, interfering with movement. The aim of this whole-genome scan was to identify quantitative trait loci (QTL) for CPD in German draft horses. We recorded clinical data for CPD in 917 German draft horses and collected blood samples from these horses. Of these 917 horses, 31 paternal half-sib families comprising 378 horses from the breeds Rhenish German, Schleswig, Saxon-Thuringian, and South German were chosen for genotyping. Each half-sib family was constituted by only one draft horse breed. Genotyping was done for 318 polymorphic microsatellites evenly distributed on all equine autosomes and the X chromosome with a mean distance of 7.5 Mb. An across-breed multipoint linkage analysis revealed chromosome-wide significant QTL on horse chromosomes (ECA) 1, 9, 16, and 17. Analyses by breed confirmed the QTL on ECA1 in South German and the QTL on ECA9, 16, and 17 in Saxon-Thuringian draft horses. For the Rhenish German and Schleswig draft horses, additional QTL on ECA4 and 10 and for the South German draft horses an additional QTL on ECA7 were found. This is the first whole-genome scan for CPD in draft horses and it is an important step toward the identification of candidate genes.
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559, Hannover, Germany.
authors.forename: E Henrike
authors.initials: EH
authors.lastname: Mittmann
authors.forename: Stefanie
authors.initials: S
authors.lastname: Mömke
authors.forename: Ottmar
authors.initials: O
authors.lastname: Distl
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D002908:Chronic Disease; D003872:Dermatitis / Q000235:genetics / Q000662:veterinary*; D005534:Foot Diseases / Q000235:genetics / Q000662:veterinary*; D055106:Genome-Wide Association Study; D005838:Genotype; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses / Q000235:genetics*; D008126:Lod Score; D008209:Lymphedema / Q000235:genetics / Q000662:veterinary*; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci