Conidial surface proteins of Metarhizium anisopliae: Source of activities related with toxic effects, host penetration and pathogenesis.
Conidial contact with an arthropod surface is the first step of the fungal penetration and infection process. However, conidia of Metarhizium anisopliae have associated components, like enzymes that could be involved in triggering the penetration process and toxic effects that have not yet been well characterized. Fungi produce many enzymes that also are toxic components found in bacteria and animal venoms and thus may be considered as potential virulence factors. In this work, we report several enzymatic activities from spore surface protein extracts. The major proteolytic activities observed in spore surface proteins (SSP) were Pr1 and Pr2 activities, in that order. According to the zymograms obtained, SSP contain different proteases. SSP contain trehalase, exo- and endo-chitinase activities, and seven different chitinase bands which have been observed in zymograms. Activities involved in protection against reactive oxygen species (ROS) were also detected. Two lipolytic enzymes were also detected in lipase zymograms. Phospholipase C activity, closely related to microbial pathogenesis, was detected for the first time in M. anisopliae conidia. These activities described could be an initial step towards understanding the mechanisms involved in the first stage of M. anisopliae infection process and its toxic effects against arthropod hosts.
Santi
Lucélia
L
Centro de Biotecnologia Universidade Federal do Rio Grande do Sul, P. O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Beys da Silva
Walter O
WO
Berger
Markus
M
Guimarães
Jorge A
JA
Schrank
Augusto
A
Vainstein
Marilene H
MH
D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology / Q000633:toxicity*; D054884:Host-Pathogen Interactions; D006868:Hydrolysis; D052981:Metarhizium / Q000737:chemistry*
D005656:Fungal Proteins