Bisabolol-induced gastroprotection against acute gastric lesions: role of prostaglandins, nitric oxide, and KATP+ channels.
The effects of Matricaria recutita and alpha-bisabolol, a bioactive component from Chamomile species, were investigated against gastric damage induced by absolute ethanol (96%, 1 mL per animal) in rats. The effects of M. recutita extract and alpha-bisabolol on gastric mucosal damage were assessed by determination of changes in mean gastric lesion area. Mechanistic studies were carried out at with 100 mg=kg alpha-bisabolol. We further examined the possible participation of prostaglandins, nitric oxide, and KATP+ channels in its mechanism. M. recutita reduced gastric damage in all doses tested. Alpha-bisabolol at oral doses of 50 and 100 mg=kg markedly attenuated the gastric lesions induced by ethanol to the extent of 87% and 96%, respectively. Pretreatments with the nitric oxide antagonist N-nitro-l-arginine methyl ester (10 mg=kg, i.p.) or with indomethacin, an inhibitor of cyclooxygenase, failed to block effectively the gastroprotective effect of alpha-bisabolol. Furthermore, the alpha-bisabolol effect was significantly reduced in rats pretreated with glibenclamide, an inhibitor of KATP+ channel activation. Thus we provide evidence that alpha-bisabolol reduces the gastric damage induced by ethanol, at least in part, by the mechanism of activation of KATP+ channels.
Bezerra
S B
SB
Department of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Leal
L K A M
LK
Nogueira
N A P
NA
Pinto
N A N
NA
Campos
A R
AR
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D031168:Matricaria / Q000737:chemistry*; D000081222:Monocyclic Sesquiterpenes; D009569:Nitric Oxide / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage*; D011453:Prostaglandins / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D012717:Sesquiterpenes / Q000008:administration & dosage*; D013272:Stomach Diseases / Q000188:drug therapy* / Q000378:metabolism
D054086:KATP Channels; D000081222:Monocyclic Sesquiterpenes; D010936:Plant Extracts; D011453:Prostaglandins; D012717:Sesquiterpenes; C004497:bisabolol; D009569:Nitric Oxide