Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1.
Mitochondrial fusion depends on the dynamin-like guanosine triphosphatase OPA1, whose activity is controlled by proteolytic cleavage. Dysfunction of mitochondria induces OPA1 processing and results in mitochondrial fragmentation, allowing the selective removal of damaged mitochondria. In this study, we demonstrate that two classes of metallopeptidases regulate OPA1 cleavage in the mitochondrial inner membrane: isoenzymes of the adenosine triphosphate (ATP)-dependent matrix AAA (ATPase associated with diverse cellular activities [m-AAA]) protease, variable assemblies of the conserved subunits paraplegin, AFG3L1 and -2, and the ATP-independent peptidase OMA1. Functionally redundant isoenzymes of the m-AAA protease ensure the balanced accumulation of long and short isoforms of OPA1 required for mitochondrial fusion. The loss of AFG3L2 in mouse tissues, down-regulation of AFG3L1 and -2 in mouse embryonic fibroblasts, or the expression of a dominant-negative AFG3L2 variant in human cells decreases the stability of long OPA1 isoforms and induces OPA1 processing by OMA1. Moreover, cleavage by OMA1 causes the accumulation of short OPA1 variants if mitochondrial DNA is depleted or mitochondrial activities are impaired. Our findings link distinct peptidases to constitutive and induced OPA1 processing and shed new light on the pathogenesis of neurodegenerative disorders associated with mutations in m-AAA protease subunits.
Ehses
Sarah
S
Institute for Genetics, University of Cologne, Germany.
Raschke
Ines
I
Mancuso
Giuseppe
G
Bernacchia
Andrea
A
Geimer
Stefan
S
Tondera
Daniel
D
Martinou
Jean-Claude
JC
Westermann
Benedikt
B
Rugarli
Elena I
EI
Langer
Thomas
T
D049069:ATP-Dependent Proteases; D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004795:Enzyme Stability; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference
D007527:Isoenzymes; D024101:Mitochondrial Proteins; D045726:Metalloproteases; C572960:OMA1 protein, mouse; D049069:ATP-Dependent Proteases; C542395:Afg3l2 protein, mouse; D008666:Metalloendopeptidases; C114583:m-AAA proteases; C477817:molecule metalloprotease-related protein-1, human; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; C498809:Opa1 protein, mouse; D000074183:ATPases Associated with Diverse Cellular Activities