Enzyme-linked immunosorbent assay for total isoflavonoids in Pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies.
Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.05-6.25 microg/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.
Pongkitwitoon
Benyakan
B
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Sakamoto
Seiichi
S
Tanaka
Hiroyuki
H
Tsuchihashi
Ryota
R
Kinjo
Junei
J
Morimoto
Satoshi
S
Putalun
Waraporn
W
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005419:Flavonoids / Q000032:analysis*; D007529:Isoflavones / Q000276:immunology*; D029908:Pueraria / Q000737:chemistry*; D011817:Rabbits
D000906:Antibodies; D005419:Flavonoids; D007529:Isoflavones; C013908:daidzin; C033607:puerarin