A molecular map of chloroquine resistance in Mali.
Plasmodium falciparum chloroquine resistance (CQR) transporter point mutation (PfCRT 76T) is known to be the key determinant of CQR. Molecular detection of PfCRT 76T in field samples may be used for the surveillance of CQR in malaria-endemic countries. The genotype-resistance index (GRI), which is obtained as the ratio of the prevalence of PfCRT 76T to the incidence of CQR in a clinical trial, was proposed as a simple and practical molecular-based addition to the tools currently available for monitoring CQR in the field. In order to validate the GRI model across populations, time, and resistance patterns, we compiled data from the literature and generated new data from 12 sites across Mali. We found a mean PfCRT 76T mutation prevalence of 84.5% (range 60.9-95.1%) across all sites. CQR rates predicted from the GRI model were extrapolated onto a map of Mali to show the patterns of resistance throughout the participating regions. We present a comprehensive map of CQR in Mali, which strongly supports recent changes in drug policy away from chloroquine.
Djimde
Abdoulaye A
AA
Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, Faculty of Medicine, Pharmacy and Odonto-Stomatology, University of Bamako, Mali, West Africa. adjimde@icermali.org
Barger
Breanna
B
Kone
Aminatou
A
Beavogui
Abdoul H
AH
Tekete
Mamadou
M
Fofana
Bakary
B
Dara
Antoine
A
Maiga
Hamma
H
Dembele
Demba
D
Toure
Sekou
S
Dama
Souleymane
S
Ouologuem
Dinkorma
D
Sangare
Cheick Papa Oumar
CP
Dolo
Amagana
A
Sogoba
Nofomo
N
Nimaga
Karamoko
K
Kone
Yacouba
Y
Doumbo
Ogobara K
OK
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics*; D006801:Humans; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000453:epidemiology*; D008302:Mali / Q000453:epidemiology; D026901:Membrane Transport Proteins / Q000235:genetics*; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011159:Population Surveillance / Q000379:methods*; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics*
D000962:Antimalarials; D016054:DNA, Protozoan; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine