Standardized MRD quantification in European ALL trials: proceedings of the Second International Symposium on MRD assessment in Kiel, Germany, 18-20 September 2008.
Assessment of minimal residual disease (MRD) has acquired a prominent position in European treatment protocols for patients with acute lymphoblastic leukemia (ALL), on the basis of its high prognostic value for predicting outcome and the possibilities for implementation of MRD diagnostics in treatment stratification. Therefore, there is an increasing need for standardization of methodologies and harmonization of terminology. For this purpose, a panel of representatives of all major European study groups on childhood and adult ALL and of international experts on PCR- and flow cytometry-based MRD assessment was built in the context of the Second International Symposium on MRD assessment in Kiel, Germany, 18-20 September 2008. The panel summarized the current state of MRD diagnostics in ALL and developed recommendations on the minimal technical requirements that should be fulfilled before implementation of MRD diagnostics into clinical trials. Finally, a common terminology for a standard description of MRD response and monitoring was established defining the terms 'complete MRD response', 'MRD persistence' and 'MRD reappearance'. The proposed MRD terminology may allow a refined and standardized assessment of response to treatment in adult and childhood ALL, and provides a sound basis for the comparison of MRD results between different treatment protocols.
Brüggemann
M
M
Department of Hematology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany. m.brueggemann@med2.uni-kiel.de
Schrauder
A
A
Raff
T
T
Pfeifer
H
H
Dworzak
M
M
Ottmann
O G
OG
Asnafi
V
V
Baruchel
A
A
Bassan
R
R
Benoit
Y
Y
Biondi
A
A
Cavé
H
H
Dombret
H
H
Fielding
A K
AK
Foà
R
R
Gökbuget
N
N
Goldstone
A H
AH
Goulden
N
N
Henze
G
G
Hoelzer
D
D
Janka-Schaub
G E
GE
Macintyre
E A
EA
Pieters
R
R
Rambaldi
A
A
Ribera
J-M
JM
Schmiegelow
K
K
Spinelli
O
O
Stary
J
J
von Stackelberg
A
A
Kneba
M
M
Schrappe
M
M
van Dongen
J J M
JJ
D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D018365:Neoplasm, Residual / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000628:therapy
D016044:Fusion Proteins, bcr-abl