Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice.
Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture-based production systems and virus-like particle (VLP)-based technologies have been established. We developed swine-origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co-expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI-TN5B1-4 (High Five). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses.
Krammer
Florian
F
Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Nakowitsch
Sabine
S
Messner
Paul
P
Palmberger
Dieter
D
Ferko
Boris
B
Grabherr
Reingard
R
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D002460:Cell Line; D006384:Hemagglutination / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism*; D007251:Influenza, Human / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D018411:Spodoptera / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000276:immunology*
D001323:Autoantibodies; D007252:Influenza Vaccines; D011994:Recombinant Proteins