Sulfate and glutathione enhanced arsenic accumulation by arsenic hyperaccumulator Pteris vittata L.
This experiment examined the effects of sulfate (S) and reduced glutathione (GSH) on arsenic uptake by arsenic hyperaccumulator Pteris vittata after exposing to arsenate (0, 15 or 30 mg As L(-1)) with sulfate (6.4, 12.8 or 25.6 mg S L(-1)) or GSH (0, 0.4 or 0.8 mM) for 2-wk. Total arsenic, S and GSH concentrations in plant biomass and arsenic speciation in the growth media and plant biomass were determined. While both S (18-85%) and GSH (77-89%) significantly increased arsenic uptake in P. vittata, GSH also increased arsenic translocation by 61-85% at 0.4 mM (p < 0.05). Sulfate and GSH did not impact plant biomass or arsenic speciation in the media and biomass. The S-induced arsenic accumulation by P. vittata was partially attributed to increased plant GSH (21-31%), an important non-enzymatic antioxidant countering oxidative stress. This experiment demonstrated that S and GSH can effectively enhance arsenic uptake and translocation by P. vittata.
Wei
Shuhe
S
Key Laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Shenyang 110116, PR China.
Ma
Lena Q
LQ
Saha
Uttam
U
Mathews
Shiny
S
Sundaram
Sabarinath
S
Rathinasabapathi
Bala
B
Zhou
Qixing
Q
D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D032495:Pteris / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism; D013431:Sulfates / Q000032:analysis / Q000378:metabolism*
D012989:Soil Pollutants; D013431:Sulfates; D005978:Glutathione; D001151:Arsenic