title: The gelsolin:calponin complex nucleates actin filaments with distinct morphologies.
abstract: Gelsolin and calponin are cytoskeletal and signalling proteins that form a tight 1:1 complex (GCC). We show that calponin within the GCC inhibits the rate of gelsolin mediated nucleation of actin polymerization. The actin-binding function of calponin is ablated within the GCC as the actin-binding site overlaps with one of the gelsolin binding sites. The structure of filaments that result from nucleation by GCC are different to those nucleated by gelsolin alone in that they are longer, loosely bundled and stain heterogeneously with phalloidin. GCC nucleated filaments appear contorted and wrap around each to form the loose bundles.
authors.affiliation: DIMNP, University of Montpellier 2 and 1, CNRS Montpellier, Place E. Bataillon CC107, 34095 Montpellier Cedex 5, France.
authors.forename: Imen
authors.initials: I
authors.lastname: Ferjani
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Fattoum
authors.forename: Nadir
authors.initials: N
authors.lastname: Bettache
authors.forename: Bastien
authors.initials: B
authors.lastname: Seantier
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Milhiet
authors.forename: Mohamed
authors.initials: M
authors.lastname: Manai
authors.forename: Yves
authors.initials: Y
authors.lastname: Benyamin
authors.forename: Claude
authors.initials: C
authors.lastname: Roustan
authors.forename: Sutherland K
authors.initials: SK
authors.lastname: Maciver
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018260:Gelsolin / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008854:Microscopy, Electron; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D000096985:Calponins