title: Fuel toxicity on Isochrysis galbana and a coastal phytoplankton assemblage: growth rate vs. variable fluorescence.
abstract: Laboratory cultures of the flagellate Isochrysis galbana and a coastal phytoplankton assemblage were used to assess the feasibility of variable fluorescence (F(v)), measured by means of fast repetition rate fluorometry, as endpoint to monitor the effect of fuel on microalgae as compared to phytoplankton growth rate. Parallel changes in cell size of I. galbana and taxonomic composition of the natural assemblage were recorded. The results presented in this investigation indicate that F(v) is a suitable endpoint to this aim, due to the ease and rapidity of the measurement, ecological relevance and sensitivity. The derived effective concentrations provided toxicity thresholds similar to, or even lower than concentrations derived from embryo-larval bioassays with marine organisms. The lowest calculated EC(10) corresponded to that of the natural assemblage, 2.5 microg eq chrysene L(-1), concentration easily exceeded in high maritime traffic areas subjected to frequent spillages.
authors.affiliation: Laboratorio de Ecoloxía Mariña, Departamento de Ecoloxía e Bioloxía Animal, Facultade de Ciencias do Mar, Universidade de Vigo, Estda. Colexio universitario s/n, 36310 Vigo (Galicia), Spain. patperez@uvigo.es
authors.forename: P
authors.initials: P
authors.lastname: Pérez
authors.forename: E
authors.initials: E
authors.lastname: Fernández
authors.forename: R
authors.initials: R
authors.lastname: Beiras
mesh_terms: D001681:Biological Assay; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development; D002965:Classification; D023381:Endpoint Determination; D004784:Environmental Monitoring / Q000379:methods; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D005648:Fuel Oils / Q000633:toxicity*; D010839:Phytoplankton / Q000187:drug effects* / Q000254:growth & development; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D005648:Fuel Oils; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D000077194:Chlorophyll A