Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine.
Cell sheet technology (CST) is based on the use of thermoresponsive polymers, poly(N-isopropylacrylamide) (PIPAAm). The surface of PIPAAms is formulated in such a way as to make its typical thickness <100 nm. In this review, we first focus on how the methods of PIPAAm-grafted surface preparations and functionalization are important to be able to harvest a functional cell sheet, to be further transplanted. Then, we present aspects of tissue mimics and three-dimensional reconstruction of a tissue in vitro. Finally, we give an overview of clinical applications and clinically relevant animal experimentations of the technology, such as cardiomyopathy, visual acuity, periodonty, oesophageal ulcerations and type 1 diabetes.
Elloumi-Hannachi
I
I
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
Yamato
M
M
Okano
T
T
D000818:Animals; D001672:Biocompatible Materials; D009202:Cardiomyopathies / Q000628:therapy; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000628:therapy; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy; D004935:Esophageal Diseases / Q000628:therapy; D006801:Humans; D008567:Membranes, Artificial; D036103:Nanotechnology; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D008567:Membranes, Artificial