title: Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions.
abstract: Frankia is an actinobacterium that fixes nitrogen under both symbiotic and free-living conditions. We identified genes upregulated in free-living nitrogen-fixing cells by using suppression subtractive hybridization. They included genes with predicted functions related to nitrogen fixation, as well as with unknown function. Their upregulation was a novel finding in Frankia.
authors.affiliation: Graduate School of Science and Engineering, Kagoshima University, Korimoto 1-21-35, Kagoshima 890-0065, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaura
authors.forename: Toshiki
authors.initials: T
authors.lastname: Uchiumi
authors.forename: Shiro
authors.initials: S
authors.lastname: Higashi
authors.forename: Mikiko
authors.initials: M
authors.lastname: Abe
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kucho
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D040161:Frankia / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D005798:Genes, Bacterial; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015854:Up-Regulation
chemical_list: D004269:DNA, Bacterial