title: Use of information visualization methods eliminating cross talk in multiple sensing units investigated for a light-addressable potentiometric sensor.
abstract: The integration of nanostructured films containing biomolecules and silicon-based technologies is a promising direction for reaching miniaturized biosensors that exhibit high sensitivity and selectivity. A challenge, however, is to avoid cross talk among sensing units in an array with multiple sensors located on a small area. In this letter, we describe an array of 16 sensing units of a light-addressable potentiometric sensor (LAPS), which was made with layer-by-layer (LbL) films of a poly(amidomine) dendrimer (PAMAM) and single-walled carbon nanotubes (SWNTs), coated with a layer of the enzyme penicillinase. A visual inspection of the data from constant-current measurements with liquid samples containing distinct concentrations of penicillin, glucose, or a buffer indicated a possible cross talk between units that contained penicillinase and those that did not. With the use of multidimensional data projection techniques, normally employed in information visualization methods, we managed to distinguish the results from the modified LAPS, even in cases where the units were adjacent to each other. Furthermore, the plots generated with the interactive document map (IDMAP) projection technique enabled the distinction of the different concentrations of penicillin, from 5 mmol L(-1) down to 0.5 mmol L(-1). Data visualization also confirmed the enhanced performance of the sensing units containing carbon nanotubes, consistent with the analysis of results for LAPS sensors. The use of visual analytics, as with projection methods, may be essential to handle a large amount of data generated in multiple sensor arrays to achieve high performance in miniaturized systems.
authors.forename: José R
authors.initials: JR
authors.lastname: Siqueira
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Maki
authors.forename: Fernando V
authors.initials: FV
authors.lastname: Paulovich
authors.forename: Carl F
authors.initials: CF
authors.lastname: Werner
authors.forename: Arshak
authors.initials: A
authors.lastname: Poghossian
authors.forename: Maria C F
authors.initials: MC
authors.lastname: de Oliveira
authors.forename: Valtencir
authors.initials: V
authors.lastname: Zucolotto
authors.forename: Osvaldo N
authors.initials: ON
authors.lastname: Oliveira
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schöning
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D005947:Glucose / Q000737:chemistry; D008027:Light; D037742:Nanotubes, Carbon; D010400:Penicillin G / Q000737:chemistry; D011199:Potentiometry / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D037742:Nanotubes, Carbon; D005947:Glucose; D010400:Penicillin G