title: Dokhwaljihwang-tang inhibits LPS-induced inflammatory cytokine production in peripheral blood mononuclear cells.
abstract: OBJECTIVE
  Dokhwaljihwang-tang, one of the prescriptions in Four Constitution medicine, has been used to treat neurological disorders. This work was aimed to examine the effect of Dokhwaljihwang-tang on lipopolysaccharide-induced inflammatory cytokine production in peripheral blood mononuclear cells from the lesser yang subjects.
  
  
  METHODS
  The inflammatory cytokines, interleukin 1 beta, interleukin 4 and tumor necrosis factor were measured using the ELISA. The interleukin 1 beta mRNA expression was evaluated with reverse transcriptase-polymerase chain reaction. The expression level of nuclear factor kappa B was examined by western blotting.
  
  
  RESULTS
  Dokhwaljihwang-tang significantly inhibited interleukin 1 beta and interleukin 4 production (p<0.05). When Dokhwaljihwang-tang was pre-treated at the dose of 1 mg/ml, it not only decreased the interleukin 1 beta mRNA expression, but also inhibited the activation of nuclear factor kappa B.
  
  
  CONCLUSIONS
  These results suggest the potential of Dokhwaljihwang-tang as a source of pharmaceutical acupuncture for neuronal inflammatory diseases.
authors.affiliation: Oriental Medical Science Center, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Seong-Gum
authors.initials: SG
authors.lastname: Ko
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Lee
authors.forename: Na-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: An
authors.forename: Noh-Yil
authors.initials: NY
authors.lastname: Myung
authors.forename: Hyejung
authors.initials: H
authors.lastname: Lee
authors.forename: Myong-Jo
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ju-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D055670:Medicine, Korean Traditional; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C548960:dokhwaljihwang-tang; D015847:Interleukin-4