Magnetic screening of the potential targeted protein of salvianolic acid B using T7 phage display library.
Salvianolic acid B is one of the effective components from the Chinese traditional drug Salvia miltiorrhiza (Danshen), which is widely used as a usual clinic drug for atherosclerosis-related disorder patients in China. But the targeting protein of salvianolic acid B is still not known. The possible targeting proteins of salvianolic acid B were explored by high throughput screening in this paper. Attached to the magnetic nanoparticles, salvianolic acid B was used for screening the high-affinity protein from the displaying cDNA peptide library phage. After biopanning, the selected protein or peptide sequences were used to explore the whole proteins containing the selected sequences in the National Center for Biotechnology Information website using blast. One of the selected phages was carried out by affinity analysis with salvianolic acid B using capillary electrophoresis (CE). The CE results indicated that the protein or peptide on the surface of the selected phages could bind the drug salvianolic acid B. The results are helpful to preliminarily explain the pharmacology of salvianolic acid B.
Liu
Shupeng
S
Institute of Biomedical Engineering, Shanghai University, Shanghai 200072, People's Republic of China. liusp@shu.edu.cn
Sun
Ye
Y
Chen
Heng
H
Song
Suxian
S
Xu
Yuhong
Y
D017123:Bacteriophage T7 / Q000235:genetics / Q000378:metabolism; D001572:Benzofurans / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D019151:Peptide Library; D010618:Phenanthrolines / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D019151:Peptide Library; D010618:Phenanthrolines; D011506:Proteins; C028972:dan-shen root extract; C076944:salvianolic acid B; D053630:Depsides