title: Sclerostin binds and regulates the activity of cysteine-rich protein 61.
abstract: Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61.
authors.affiliation: Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Theodore A
authors.initials: TA
authors.lastname: Craig
authors.forename: Resham
authors.initials: R
authors.lastname: Bhattacharya
authors.forename: Debabrata
authors.initials: D
authors.lastname: Mukhopadhyay
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
mesh_terms: D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D055515:Cysteine-Rich Protein 61 / Q000235:genetics / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; C501848:CCN1 protein, human; D055515:Cysteine-Rich Protein 61; D005819:Genetic Markers; C422903:SOST protein, human