Effect of a negatively charged lipid on membrane-lacticin Q interaction and resulting pore formation.
Lacticin Q is an antimicrobial peptide that forms pores on membranes. We investigated effects of negatively charged lipids on the binding and pore formation of lacticin Q with liposomes by surface plasmon resonance analysis and fluorescence dye leakage experiments respectively. Negatively charged lipids accelerated the binding of lacticin Q on the membranes and the resulting pore formation. However, the acceleration was not an essential factor in the killing activity of lacticin Q, since pore-forming activities against electrically neutral and negatively charged liposomes occurred similarly.
Yoneyama
Fuminori
F
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
Shioya
Kouki
K
Zendo
Takeshi
T
Nakayama
Jiro
J
Sonomoto
Kenji
K
D001430:Bacteriocins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D008055:Lipids / Q000737:chemistry* / Q000494:pharmacology*; D008081:Liposomes / Q000378:metabolism; D016062:Porosity / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects
D001430:Bacteriocins; D005452:Fluoresceins; D008055:Lipids; D008081:Liposomes; C548356:lacticin Q, Lactococcus lactis; C007740:fluorexon