A case of personal identification due to detection of rare DNA types from seminal stain.
Following a rape incident in an apartment in Japan, we were requested to perform a DNA analysis on a body fluid stain left on a bath towel to determine whether it could be attributed to the suspect. The acid phosphatase and prostatic-specific antigen tests confirmed it to be a seminal stain. Based on the DNA analysis by autosomal and Y-chromosome short tandem repeat (STR) systems, no inconsistency was found with the profile of the suspect with African ancestry. In this case, allele 21 of DYS390 at the Y-STR locus was examined, as it is reported to have a distinctly lower frequency in the Japanese population. Furthermore, the haplotype combinations of Y-STR at the DYS389I, DYS389II and DYS390 loci are powerful for personal identification, as these have not yet been found in the Japanese population.
Tsutsumi
Hirofumi
H
Department of Legal Medicine, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan. tsutsumi@dent.nihon-u.ac.jp
Komuro
Toshinobu
T
Mukoyama
Rei
R
Izawa
Hikaru
H
Tie
Jian
J
Uchigasaki
Seisaku
S
D044383:Black People / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D018732:Forensic Anthropology / Q000379:methods*; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats; D011902:Rape; D012661:Semen / Q000737:chemistry*; D017422:Sequence Analysis, DNA