title: Effect of isoflavone-enriched milk on bone mass in ovariectomized rats.
abstract: This study examined the effect of isoflavone-enriched milk on bone loss in ovariectomized (OVX) rats. Thirty 6-week-old Sprague-Dawley female rats were divided into two groups: sham-operated and OVX. The OVX group was subdivided into three dietary groups (OVX, non-isoflavone-enriched milk; OVX+Iso, isoflavone-enriched milk; and OVX+Iso+Vit+Ca, isoflavone-, vitamins D and K-, and Ca-enriched mik). After 19 weeks of feeding, the food efficiency ratio and body weight gain in the sham-operated group were significantly lower compared with those in the other groups. The bone alkaline phosphatase and total alkaline phosphatase activities were significantly higher in isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca) when compared with the sham-operated group. Urinary excretions of deoxypyridinoline and hydroxyproline were significantly higher with ovariectomy, but mostly normalized in the OVX+Iso and OVX+Iso+Vit+Ca groups. The rats in the OVX+Iso and OVX+Iso+Vit+Ca groups showed higher femur and tibia weights. A significant increase was found in bone density of femur and trabecular bone area in the OVX+Iso+Vit+Ca group, which almost reached that of the sham-operated group, whereas no difference was found among the OVX and OVX+Iso groups. The histological areas of the proximal tibia sections showed highly filled trabecular bone in both isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca). The present study indicated that isoflavone-enriched milk may have a partial preventive effect on ovariectomy-induced bone loss; however, vitamins D and K and Ca enrichment with isoflavone may enhance effectiveness for increasing bone mass in OVX rats.
authors.affiliation: Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea.
authors.forename: Byung-Ju
authors.initials: BJ
authors.lastname: Jeon
authors.forename: Joungjwa
authors.initials: J
authors.lastname: Ahn
authors.forename: Hae-Soo
authors.initials: HS
authors.lastname: Kwak
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000652:urine; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D006909:Hydroxyproline / Q000652:urine; D007529:Isoflavones / Q000008:administration & dosage*; D010024:Osteoporosis / Q000178:diet therapy* / Q000188:drug therapy* / Q000503:physiopathology; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D041942:Soy Milk / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D007529:Isoflavones; C036020:deoxypyridinoline; D000469:Alkaline Phosphatase; D006909:Hydroxyproline