title: Sequence analysis of plasmid pIR52-1 from Lactobacillus helveticus R0052 and investigation of its origin of replication.
abstract: Lactobacillus helveticus R0052 is a bacterium used in commercial probiotic preparations. R0052 contains a small, cryptic plasmid comprised of eight open reading frames, four of which encode proteins of unknown function. Based on the sequence of the replication initiation protein RepA, pIR52-1 is a member of the recently described RepA_N family of Gram-positive theta-replicating plasmids. The repA gene of pIR52-1 is the minimal origin of replication for L. helveticus and other Lactobacillus hosts. Additionally, pIR52-1 belongs to a subgroup of the RepA_N plasmid family which have RepA proteins of high amino acid identity and a conserved, non-coding element upstream of repA which, in pIR52-1, is responsible for the control of plasmid copy number and contributes to plasmid maintenance.
authors.affiliation: Department of Research and Development, Institut Rosell Inc., 6100 Avenue Royalmount, Montreal, Que., Canada. karen@operonscript.com <karen@operonscript.com>
authors.forename: Karen E
authors.initials: KE
authors.lastname: Hagen
authors.forename: Cody A
authors.initials: CA
authors.lastname: Tramp
authors.forename: Eric
authors.initials: E
authors.lastname: Altermann
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Welker
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Tompkins
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D018628:Gene Dosage; D005822:Genetic Vectors / Q000235:genetics; D052198:Lactobacillus helveticus / Q000235:genetics*; D048191:Lactiplantibacillus plantarum / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins