Anti-tobacco mosaic virus (TMV) Quassinoids from Brucea javanica (L.) Merr.
Two new quassinoids, javanicolide E (1) and javanicolide F (2), along with fifteen known C-20 quassinoids were isolated from the seeds of Brucea javanica (L.) Merr. The antitobacco mosaic virus (TMV) activity of these quassinoids was screened by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the seventeen quassinoids showed potent anti-TMV activity. Among them, eight compounds, brusatol (3), bruceine B (4), bruceoside B (5), yadanzioside I (6), yadanzioside L (7), bruceine D (8), yadanziolide A (9), and aglycone of yadanziolide D (17), showed strong antiviral activities, with IC(50) values in the range of 3.42-5.66 microM, and were much more effective than the positive control, ningnanmycin (IC(50) = 117.3 microM). The antiviral structure-activity relationships of quassinoids against TMV were also discussed.
Yan
Xiao-Hui
XH
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
Chen
Jia
J
Di
Ying-Tong
YT
Fang
Xin
X
Dong
Jia-Hong
JH
Sang
Peng
P
Wang
Yue-Hu
YH
He
Hong-Ping
HP
Zhang
Zhong-Kai
ZK
Hao
Xiao-Jiang
XJ
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D032269:Brucea / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D036702:Quassins / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014027:Tobacco Mosaic Virus / Q000187:drug effects*
D000998:Antiviral Agents; D010936:Plant Extracts; D036702:Quassins