Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells.
Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.
Konishi
Tomoyuki
T
Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Miyazaki
Yasumasa
Y
Yamakawa
Seiyei
S
Iwai
Hiroaki
H
Satoh
Shinobu
S
Ishii
Tadashi
T
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D019751:Intramolecular Transferases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D012275:Oryza / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology / Q000235:genetics*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D011994:Recombinant Proteins; D014539:Uridine Diphosphate Sugars; C040817:uridine diphosphate arabinose; D019751:Intramolecular Transferases