Development of microfluidic-based heterogeneous immunoassays.
Microfluidic-based heterogeneous immunoassays are reviewed in this article by first introducing the principle of immunoassay, followed by a discussion of microfluidic-based technology. Microfabrication, surface modification, solution dispensing and detection technology are discussed and their applications to biomolecular detection reviewed. In the future, improved manufacturing processes and integrated assay systems in an automatic fashion with a reduced assay time and reagent consumption will allow for the effective detection of biomolecules that are of interest in medical diagnostics, drug discovery and bioterrorism.
Lin
Frank Yung Harn
FY
Southlake Regional Health Centre, Newmarket, Ontario, Canada. franklin088@yahoo.ca
Gao
Yali
Y
Li
Dongqing
D
Sherman
Philip Martin
PM
D004563:Electrochemistry / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods