Electrochemical nanobiosensor for express diagnosis of acute myocardial infarction in undiluted plasma.
The myocardial infarction biomarker myoglobin was quantified at the biological level in undiluted plasma using developed electrochemical nanosensors with immobilized anti-myoglobin. Method for cardiac myoglobin detection is based on direct electron transfer between Fe(III)-heme and electrode surface modified with gold nanoparticles/didodecyldimethylammonium bromide (DDAB/Au) and antibodies. The procedure of myoglobin detection was optimized (pH, incubation times and characteristics of electrodes) to express determination of the marker in serum or plasma. Plasma of healthy donors and patients with acute myocardial infarction (AMI) was analyzed using electrochemical immunosensors and RAMP immunoassay. Square wave voltammetry cathodic peak of cardiac myoglobin reduction was found to be proportional to myoglobin quantity in plasma as determined by RAMP. The method proposed does not require signal enhancement or amplification; nor does it require labeled secondary antibodies. Immunosensor has a detection limit of 10 ng/ml (0.56 nM) and a broad range of working concentrations (10-1780 ng/ml; 0.56-100 nM). The whole procedure takes 30 min and can be used for express diagnosis of acute myocardial infarction.
Suprun
Elena
E
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
Bulko
Tatiana
T
Lisitsa
Alexander
A
Gnedenko
Oksana
O
Ivanov
Alexis
A
Shumyantseva
Victoria
V
Archakov
Alexander
A
D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D009211:Myoglobin / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D009211:Myoglobin