title: Crystallization and preliminary X-ray analysis of neoagarobiose hydrolase from Saccharophagus degradans 2-40.
abstract: Many agarolytic bacteria degrade agar polysaccharide into the disaccharide unit neoagarobiose [O-3,6-anhydro-alpha-L-galactopyranosyl-(1-->3)-D-galactose] using various beta-agarases. Neoagarobiose hydrolase is an enzyme that acts on the alpha-1,3 linkage in neoagarobiose to yield D-galactose and 3,6-anhydro-L-galactose. This activity is essential in both the metabolism of agar by agarolytic bacteria and the production of fermentable sugars from agar biomass for bioenergy production. Neoagarobiose hydrolase from the marine bacterium Saccharophagus degradans 2-40 was overexpressed in Escherichia coli and crystallized in the monoclinic space group C2, with unit-cell parameters a = 129.83, b = 76.81, c = 90.11 A, beta = 101.86 degrees . The crystals diffracted to 1.98 A resolution and possibly contains two molecules in the asymmetric unit.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Saeyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Jonas Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Ha
authors.forename: Jina
authors.initials: J
authors.lastname: Jung
authors.forename: Dong Hae
authors.initials: DH
authors.lastname: Shin
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
authors.forename: In Geol
authors.initials: IG
authors.lastname: Choi
mesh_terms: D000362:Agar / Q000378:metabolism; D034241:Alteromonadaceae / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers / Q000235:genetics; D004186:Disaccharidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D000362:Agar; D004186:Disaccharidases; C039308:neoagarobiose hydrolase