[Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex].
The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.
Liao
Yi
Y
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Zhang
Wei-Lin
WL
Yuan
Yan-Hong
YH
Shi
Quan-Wei
QW
Li
Su-Ping
SP
Yan
Rong
R
Wang
Zhi-Cheng
ZC
Dai
Ke-Sheng
KS
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D009154:Mutation; D010957:Plasmids; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D019038:Platelet Glycoprotein GPIb-IX Complex; D011994:Recombinant Proteins