Dapdiamides, tripeptide antibiotics formed by unconventional amide ligases.
Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A-E (1-5). The structures were established through 2D-NMR experiments and mass spectrometry, as well as the synthesis of dapdiamide A (1). Transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. The dapdiamide family's promiscuous biosynthetic pathway contains two unconventional amide ligases that are predicted to couple its constituent monomers.
Dawlaty
Jessica
J
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Zhang
Xiaorong
X
Fischbach
Michael A
MA
Clardy
Jon
J
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D044084:Erwinia amylovora / Q000235:genetics* / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry; D020636:Pantoea / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009842:Oligopeptides; D008025:Ligases