title: Overexpression of Sall1 in vivo leads to reduced body weight without affecting kidney development.
abstract: Human SALL1 is a homologue of the Drosophila region-specific homeotic gene sal, and is also known as a causative gene for Townes-Brocks syndrome, which is characterized by multi-organ malformations. We previously demonstrated that mouse Sall1 plays a crucial role in ureteric bud invasion during kidney development, and possibly in nephron progenitor cells in the metanephric mesenchyme. To gain insights into the Sall1 functions in the kidney and other tissues, we generated R26Sall1 mice, in which Rosa26 locus stop sequences flanked by two loxP sites were located upstream of the Sall1 cDNA. This allele allowed exogenous Sall1 expression in a Cre recombinase-dependent manner. R26Sall1 mice were first crossed with CAGCre mice, which expressed Cre recombinase ubiquitously during embryogenesis. Mice expressing Sall1 ubiquitously were smaller in size compared with mice of other genotypes. We then crossed R26Sall1 mice with Six2Cre mice expressing Cre recombinase in the metanephric mesenchyme during kidney development. However, no kidney defects were observed. Taken together, overexpression of Sall1 does not affect kidney development, but does lead to a reduced body weight, suggesting that the optimal dosage of Sall1 is required for normal mouse development.
authors.affiliation: Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Sayoko
authors.initials: S
authors.lastname: Fujimura
authors.forename: Chiyoko
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nishinakamura
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001006:Anus, Imperforate / Q000235:genetics / Q000378:metabolism; D001835:Body Weight; D004423:Ear / Q000002:abnormalities; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D017880:Limb Deformities, Congenital / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: C491254:Sall1 protein, mouse; D014157:Transcription Factors