title: Chemically induced cardiomyogenesis of mouse embryonic stem cells.
abstract: A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of alpha-myosine heavy chain (alpha-MHC) promoter (palpha-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80 % increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets.
authors.affiliation: University of Cologne, Department of Chemistry, Greinstrasse 4, 50939 Cologne, Germany. berkessel@uni-koeln.de
authors.forename: Albrecht
authors.initials: A
authors.lastname: Berkessel
authors.forename: Bianca
authors.initials: B
authors.lastname: Seelig
authors.forename: Silke
authors.initials: S
authors.lastname: Schwengberg
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.forename: Agapios
authors.initials: A
authors.lastname: Sachinidis
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002930:Cinchona Alkaloids / Q000737:chemistry / Q000494:pharmacology; D015195:Drug Design; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D013890:Thiourea / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002930:Cinchona Alkaloids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D013890:Thiourea