title: Proteomic analysis of regenerating mouse liver following 50% partial hepatectomy.
abstract: BACKGROUND
  Although 70% (or 2/3) partial hepatectomy (PH) is the most studied model for liver regeneration, the hepatic protein expression profile associated with lower volume liver resection (such as 50% PH) has not yet been reported. Therefore, the aim of this study was to determine the global protein expression profile of the regenerating mouse liver following 50% PH by differential proteomics, and thereby gaining some insights into the hepatic regeneration mechanism(s) under this milder but clinically more relevant condition.
  
  
  RESULTS
  Proteins from sham-operated mouse livers and livers regenerating for 24 h after 50% PH were separated by SDS-PAGE and analyzed by nanoUPLC-Q-Tof mass spectrometry. Compared to sham-operated group, there were totally 87 differentially expressed proteins (with 50 up-regulated and 37 down-regulated ones) identified in the regenerating mouse livers, most of which have not been previously related to liver regeneration. Remarkably, over 25 differentially expressed proteins were located at mitochondria. Several of the mitochondria-resident proteins which play important roles in citric acid cycle, oxidative phosphorylation and ATP production were found to be down-regulated, consistent with the recently-proposed model in which the reduction of ATP content in the remnant liver gives rise to early stress signals that contribute to the onset of liver regeneration. Pathway analysis revealed a central role of c-Myc in the regulation of liver regeneration.
  
  
  CONCLUSIONS
  Our study provides novel evidence for mitochondria as a pivotal organelle that is connected to liver regeneration, and lays the foundation for further studies on key factors and pathways involved in liver regeneration following 50% PH, a condition frequently used for partial liver transplantation and conservative liver resection.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, 1st Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, PR China.
authors.forename: Hongcui
authors.initials: H
authors.lastname: Cao
authors.forename: Jiong
authors.initials: J
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Jia
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yang
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Pan
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Guoping
authors.initials: G
authors.lastname: Sheng
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Pan
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li