Late phase of L-LTP elicited in isolated CA1 dendrites cannot be transferred by synaptic capture.
In the CA1 region of mouse hippocampal slices, a strong tetanic stimulation triggers a long-lasting long-term potentiation (L-LTP), which requires transcription for the development of its late phase. Nevertheless, we were able to elicit such an L-LTP in CA1 dendrites separated from their somas provided that we restricted our investigations to isolated dendrites where a very robust early LTP was triggered. This particular type of L-LTP, which relied on translation of preexisting messenger RNAs - as it was blocked by anisomycin - could not be captured by another pathway activated only by a weak tetanic stimulation. This suggests that the plasticity-related proteins resulting from translation of messenger RNAs in dendrites cannot pass from the synaptic site where they were synthesized to another one.
Villers
Agnès
A
Laboratory of Neurosciences, University of Mons, B-7000 Mons, Belgium.
Godaux
Emile
E
Ris
Laurence
L
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology
D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D000841:Anisomycin