title: Butin reduces oxidative stress-induced mitochondrial dysfunction via scavenging of reactive oxygen species.
abstract: This study investigated the cytoprotective effect of butin, a flavonoid, on hydrogen peroxide (H(2)O(2))-induced mitochondrial dysfunction. Electron spin resonance (ESR) spectrometry revealed butin's significant scavenging effects on superoxide radicals and hydroxyl radicals. When H(2)O(2) was used to induce an increase in mitochondrial reactive oxygen species (ROS) in Chinese hamster lung fibroblast (V79-4) cells, butin treatment decreased high level of ROS. Butin also attenuated intracellular Ca(2+) levels that have been induced by H(2)O(2). Furthermore, butin recovered ATP levels and succinate dehydrogenase activity that had been decreased by H(2)O(2) treatment. We conclude these results suggest butin decreased mitochondrial ROS accumulation, balanced intracellular Ca(2+) levels, and improved mitochondrial energy production, thus recovering mitochondrial function.
authors.affiliation: School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, Republic of Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Weon Young
authors.initials: WY
authors.lastname: Chang
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Maeng
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.forename: In Kyung
authors.initials: IK
authors.lastname: Lee
authors.forename: Bum Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D003281:Contraceptives, Postcoital / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D003281:Contraceptives, Postcoital; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; C051437:butin; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase; D002118:Calcium