A transcriptome study of the QseEF two-component system and the QseG membrane protein in enterohaemorrhagic Escherichia coli O157 : H7.
QseE is a sensor kinase that responds to epinephrine, sulfate and phosphate. QseE constitutes a two-component signalling system together with the QseF sigma(54)-dependent response regulator. Encoded within the same operon as qseEF is the qseG gene, which encodes a membrane protein involved in the translocation of a type III secretion effector protein of enterohaemorrhagic Escherichia coli (EHEC) into epithelial cells. The qseEGF genes also form an operon with the glnB gene, which encodes the E. coli nitrogen sensor PII protein. Here we report a transcriptome analysis comparing qseE, qseF andqseG single mutants with the wild-type strain. This study revealed that the proteins encoded by these genes play a modest but significant role in iron uptake. Although QseEFG regulate genes involved in nitrogen utilization, these proteins do not play a notable role in nitrogen metabolism. In addition, QseEFG regulate transcription of the rcsBC and phoPQ two-component systems, linking several signal transduction pathways. The similarity of the microarray profiles of these mutants also indicates that these proteins work together. These data indicate that QseEFG are involved in the regulation of virulence and metabolism in EHEC.
Reading
Nicola C
NC
Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9048, USA.
Rasko
David
D
Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9048, USA.
Torres
Alfredo G
AG
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1070, USA.
Sperandio
Vanessa
V
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9048, USA.|Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9048, USA.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019453:Escherichia coli O157 / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000235:genetics* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C540351:QseE protein, E coli; C540352:QseF protein, E coli; D011941:Receptors, Adrenergic; C540353:YfhG protein, E coli; D007501:Iron; D009584:Nitrogen