Effects of static magnetic field exposure on antioxidative enzymes activity and DNA in rat brain.
The present study was undertaken in order to investigate the effects of static magnetic field (SMF) exposure on the antioxidative enzymes activity, malondialdehyde (MDA) concentration and DNA oxidation in male rat brain. The exposure of rats to SMF (128 mT, 1 h/day during 30 consecutive days) decreased the glutathione peroxidase (GPx; -39%, p < 0.05), CuZn superoxide dismutase (CuZn-SOD; -35%, p < 0.05) and catalase (-59%, p < 0.05) activities in frontal cortex. The same treatment decreased the CuZn-SOD (-51%, p < 0.05) and Mn-SOD (-13%, p < 0.05) activities in hippocampus. However, the glutathione levels remained unchanged in the both brain structures. In the hippocampus, SMF exposure increased MDA concentration (+32%, p < 0.05). Interestingly, exposed-rats to SMF displayed a significant increase of metallothioneins level in frontal cortex (+100%, p < 0.05), while the 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) concentration remained unaffected, indicating the absence of DNA oxidation. Our results indicated that sub-chronic exposure to SMF induced oxidative stress in rat hippocampus and frontal cortex. Metallothionein induction protected probably DNA against oxidative damage.
Amara
Salem
S
Laboratoire de Physiologie Animale, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia. amara_salem_fsb@yahoo.fr
Douki
Thierry
T
Garel
Catherine
C
Favier
Alain
A
Sakly
Mohsen
M
Rhouma
Khémais B
KB
Abdelmelek
Hafedh
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000201:enzymology* / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004574:Electromagnetic Fields; D005625:Frontal Lobe / Q000201:enzymology / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D008668:Metallothionein; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine; D005978:Glutathione