Integrating carbon nanotubes and lipid bilayer for biosensing.
Membrane proteins, which are the target of most drugs, are implicated in many critical cellular functions such as signal transduction, bioelectricity, exocytosis and endocytosis. Therefore, developing techniques to investigate the functions of membrane proteins is obviously important. Here, we have developed a novel system by integrating artificial lipid bilayer (biomimetic membrane) with single-walled carbon nanotube networks (SWNT-net) based field-effect transistor (FET), and demonstrated that such hybrid nanoelectronic biosensors can specifically and electronically detect the presence and dynamic activities of ionophores (specifically, gramicidin and calcimycin) in their native lipid environment. This technique can potentially be used to examine other membrane proteins (e.g. ligand-gated ion channels, receptors, membrane insertion toxins, and antibacterial peptides) for the purposes of biosensing, fundamental studies, or high throughput drug screening.
Huang
Yinxi
Y
School of Chemical and Biomedical Engineering, Bioengineering Division, Nanyang Technological University, 70 Nanyang Drive, Block N1.3, Singapore 637457, Singapore.
Palkar
Preeti Vikas
PV
Li
Lain-Jong
LJ
Zhang
Hua
H
Chen
Peng
P
D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003217:Conductometry / Q000379:methods*; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D018511:Systems Integration
D008051:Lipid Bilayers; D008565:Membrane Proteins; D037742:Nanotubes, Carbon