The protective effect of sodium hyaluronate on the cartilage of rabbit osteoarthritis by inhibiting peroxisome proliferator-activated receptor-gamma messenger RNA expression.
OBJECTIVE
The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-gamma) in cartilage of rabbit osteoarthritis (OA) model.


METHODS
Forty eight white rabbits were randomly divided into A, B, and C groups. Group A was normal control group, B and C groups underwent unilateral anterior cruciate ligament transection (ACLT). The rabbits in group B were injected normal saline after ACLT; and Group C received intra-articular 1% sodium hyaluronate (HA) injection 5 weeks after surgery, 0.3 mL once a week. At 11th week after surgery, all the rabbits were sacrificed. The cartilage changes on the medial femoral condyles were graded separately. Cartilage sections were stained with safranin-O and HE, and messenger RNA (mRNA) expression of PPAR-gamma was detected by using real time polymerase chain reaction (Real Time-PCR).


RESULTS
Cartilage degeneration in group B was significantly more severe than in A and C injection group. The grey value of Safranin-O of B group was higher than A and C groups. Expression of PPAR-gamma mRNA in group B was higher than group A and C.


CONCLUSIONS
This study shows that Na-HA has a protective effect on articular cartilage degeneration, and the inhibitory effect on the PPAR-gamma mRNA expression may be one of therapeutic mechanism of Na-HA.
Zhou
Jian-lin
JL
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. zhoujianlin1688@yahoo.com.cn
Liu
Shi-qing
SQ
Qiu
Bo
B
Hu
Qiong-jie
QJ
Ming
Jiang-hua
JH
Peng
Hao
H
D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006820:Hyaluronic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D008853:Microscopy; D010003:Osteoarthritis / Q000188:drug therapy* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055675:Viscosupplements / Q000494:pharmacology* / Q000627:therapeutic use
D047495:PPAR gamma; D012333:RNA, Messenger; D055675:Viscosupplements; D006820:Hyaluronic Acid