Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen.
A 16 kDa buckwheat protein (BWp16) is a major allergen responsible for immediate hypersensitivity reactions including anaphylaxis. A deletion mutant of BWp16 (rBWp16DeltaN) was overproduced and purified and was shown to be immunologically active. A three-wavelength MAD data set was collected from a crystal of selenomethionine-labelled rBWp16DeltaN. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 28.39, b = 31.54, c = 32.20 A, alpha = 111.92, beta = 108.91, gamma = 98.74 degrees . One monomer was expected to be present in the asymmetric unit based on the calculated Matthews coefficient of 1.76 A(3) Da(-1).
Kezuka
Yuichiro
Y
Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Iwate 028-3694, Japan.
Itagaki
Takashi
T
Satoh
Rie
R
Teshima
Reiko
R
Nonaka
Takamasa
T
D000485:Allergens / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D019612:Fagopyrum / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017384:Sequence Deletion
D000485:Allergens; D017931:DNA Primers; D010940:Plant Proteins; D011994:Recombinant Proteins