[Comparative study on the immunogenicity of human homologous cardiac valve and great artery homograft cryopreserved with liquid nitrogen].
OBJECTIVE
To study the differences on the immunogenicity of the leaflets, arterial wall and myocardium of conduit valved homograft (CVH) cryopreserved with liquid nitrogen.


METHODS
Mono-cell suspension of leaflets cells and arterial cells of CVH were respectively co-cultured with human lymphatic cells whose blood groups were the same with that of CVH donors. Expressive levels of CD25 and HLA-DR of these lymphatic cells were detected by flow cytometry in the different cultural duration and compared with that of lymph cells alone cultured (comparative group).


RESULTS
The immunogenicity of CVH artery walls was more severe than that of CVH leaflets, and expressive level of whose CD25 and HLA-DR was higher. The immunogenicity of CVH myocardium was not studied because the myocardial cell suspension were not be acquired in this study.


CONCLUSIONS
It is proved that in vitro experimental study that the immunogenicity of arterial walls of cryopreserved CVH is more severe than that of leaflets.
Yu
Jian-Hua
JH
Department of Cardiovascular Surgery, Shandong University Qilu Hospitial, Jinan 250012, China. Yujiahua1965@126.com
Guo
Hong-Wei
HW
Liu
Mei-Ming
MM
Zhang
Gong
G
Wu
Shu-Ming
SM
D001158:Arteries / Q000276:immunology*; D015925:Cryopreservation; D006684:HLA-DR Antigens / Q000378:metabolism; D006351:Heart Valves / Q000276:immunology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D009584:Nitrogen; D014184:Transplantation, Homologous
D006684:HLA-DR Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D009584:Nitrogen