[The functions of GLI3 and EN1 genes in idiopathic congenital talipes equinovarus].
To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus (ICTEV), we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment. Meanwhile, the correlation between the rat En1 and ICTEV was evaluated by RT-PCR, immunohistochemistry, and Western blotting analyses. The result from P-Match software prediction showed that only one of the three possible En1 binding sites in Gli3 promoter region was interacted directly with En1 in vivo, which was confirmed by ChIP analysis. The results from RT-PCR, immunohistochemistry and Western blotting analyses suggested that En1 was down-regulated in ICTEV model rats compared to the controls. Our results indicated that En1 might be the negative regulatory element in the upstream of Gli3. The low expression level of EN1 in ICTEV could contribute to the up-regulation of GLI3, which led to the genesis of ICTEV.
Cao
Dong-Hua
DH
Department of Medical Genetics, China Medical University, Shenyang 110001, China. dhcao427@sina.com
Wang
Qian
Q
Lin
Chang-Kun
CK
Wang
Zheng-Dong
ZD
Zhang
Xuan
X
Jin
Chun-Lian
CL
D000818:Animals; D001483:Base Sequence; D003025:Clubfoot / Q000196:embryology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D000074290:Zinc Finger Protein Gli3
C546743:EN1 protein, rat; C546742:GLI3 protein, rat; D018398:Homeodomain Proteins; D051741:Kruppel-Like Transcription Factors; D000074290:Zinc Finger Protein Gli3