Prostate stem cell antigen mRNA in peripheral blood as a potential predictor of biochemical recurrence in high-risk prostate cancer.
OBJECTIVE
To determine whether the presence of prostate stem cell antigen (PSCA) mRNA in peripheral blood can predict biochemical recurrence (BCR) after radical prostatectomy in patients with high-risk prostate cancer.


METHODS
High-risk disease was defined based on the presence of any one of the following three risk factors: prostate-specific antigen (PSA) > or = 20 ng/ml, a biopsy Gleason score (GS) > or = 8, or clinical stage > or = T2c. Nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect PSCA mRNA-bearing cells in peripheral blood. The relationship between PSCA detection and BCR after surgery was evaluated.


RESULTS
Of the 250 patients, 103 (36.8%) with high-risk disease were included in the analysis. PSCA-mRNA was detected in 17 (16.5%) of these patients. Patients with high GS (> or = 7) tended to be PSCA-mRNA positive status (P = 0.045). Over a median 23 months of follow-up (range 3-47 months), BCR developed in 27 patients (26.2%). Cox regression hazards model analysis revealed that a RT-PCR PSCA positivity (HR, 4.549; 95%CI, 1.685-12.279; P = 0.003) independently increased the risk of BCR.


CONCLUSIONS
The presence of PSCA mRNA in peripheral blood is a significant predictor of BCR after radical prostatectomy in high-risk prostate cancer.
Joung
Jae Young
JY
Urologic Oncology Clinic, Center for Specific Organs Cancer, National Cancer Center, Goyang, Republic of Korea.
Cho
Kang Su
KS
Kim
Jeong Eun
JE
Seo
Ho Kyung
HK
Chung
Jinsoo
J
Park
Weon Seo
WS
Choi
Moon Kyong
MK
Lee
Kang Hyun
KH
D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D009364:Neoplasm Recurrence, Local / Q000097:blood* / Q000175:diagnosis; D011237:Predictive Value of Tests; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D012333:RNA, Messenger; D012307:Risk Factors
D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C110843:PSCA protein, human; D012333:RNA, Messenger