title: Verticilide, a new ryanodine-binding inhibitor, produced by Verticillium sp. FKI-1033.
abstract: A new ryanodine-binding inhibitor, verticilide, was isolated from the cultured broth of a fungus, Verticillium sp. FKI-1033. It is a 24-membered ring cyclic depsipeptide, its structure being elucidated as cyclo[(2R)-2-hydroxyheptanoyl-N-methyl- L-alanyl](4). Verticilide inhibited ryanodine binding to ryanodine receptors in the cockroach at an IC(50) value of 4.2 microM, whereas inhibition against mouse ryanodine receptors was weak (IC(50)=53.9 microM).
authors.affiliation: Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan. shiomi@lisci.kitasato-u.ac.jp
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Matsui
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kakei
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Rokuro
authors.initials: R
authors.lastname: Masuma
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hatano
authors.forename: Noriko
authors.initials: N
authors.lastname: Arai
authors.forename: Miki
authors.initials: M
authors.lastname: Isozaki
authors.forename: Haruo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Susumu
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Andreas
authors.initials: A
authors.lastname: Turberg
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003058:Cockroaches; D047630:Depsipeptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D008967:Molecular Biology; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D011485:Protein Binding; D012433:Ryanodine / Q000037:antagonists & inhibitors*; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D013329:Structure-Activity Relationship; D020172:Verticillium / Q000378:metabolism*
chemical_list: D047630:Depsipeptides; D019837:Ryanodine Receptor Calcium Release Channel; C522755:verticilide; D012433:Ryanodine