Proteasomal activity in skeletal muscle: a matter of assay design, muscle type, and age.
The ubiquitin-proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.
Strucksberg
Karl-Heinz
KH
Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
Tangavelou
Karthikeyan
K
Schröder
Rolf
R
Clemen
Christoph S
CS
D000367:Age Factors; D000818:Animals; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*
D046988:Proteasome Endopeptidase Complex