Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants.
Our previous studies showed that immunization with recombinant paramyosin from Trichinella spiralis (rTs-Pmy) formulated with Freund's adjuvant significantly reduced larval burden in mice after T. spiralis larval challenge. Since Freund's adjuvant is toxic and not a suitable adjuvant for clinical vaccine trials, we evaluated the ability of the adjuvants Montanide ISA206 and ISA720 to stimulate immune responses during rTs-Pmy immunization and to enhance protective immunity. The results revealed that immunization of BALB/c mice with rTs-Pmy formulated with either ISA206 or ISA720 triggered Th1 and Th2 immune responses similar to those produced by the conventional Freund's adjuvant formulation and also provided a similar level of protection against T. spiralis larval challenge. This indicates that the recombinant Ts-Pmy formulated with Montanide ISA206 or ISA720 may be an effective and safety vaccine strategy for trichinellosis.
Yang
Jing
J
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, 10 Xitoutiao, You An Men, Beijing 100069, PR China.
Gu
Yuan
Y
Yang
Yaping
Y
Wei
Junfei
J
Wang
Shaohua
S
Cui
Shijuan
S
Pan
Jin
J
Li
Qiang
Q
Zhu
Xinping
X
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009132:Muscles / Q000469:parasitology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014335:Tropomyosin / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014335:Tropomyosin