title: Novel fructopyranose oligosaccharides isolated from fermented beverage of plant extract.
abstract: Four oligosaccharides containing a fructopyranosyl residue have been found from fermented beverage of plant extract and isolated from the beverage using carbon-Celite column chromatography and preparative high performance liquid chromatography. Structure confirmation of the saccharides was provided by methylation analysis, MALDI-TOF-MS and NMR measurements. These saccharides were identified as oligosaccharides of fructopyranoside series; beta-D-fructopyranosyl-(2-->6)-D-fructofuranose (1), beta-D-fructopyranosyl-(2-->1)-D-fructopyranose (2), beta-D-fructopyranosyl-(2-->1)-beta-D-fructofuranosyl-(2<-->1)-alpha-D-glucopyranoside (3), and beta-D-fructopyranosyl-(2-->6)-alpha-D-glucopyranosyl-(1<-->2)-beta-D-fructofuranoside (4). Saccharides 3 and 4 among novel saccharides 1, 3, and 4 were named 'pyrano-1-kestose (pyrano-isokestose)' and 'pyrano-neokestose', respectively.
authors.affiliation: General Institute of Ohtakakohso, Co., Ltd, Otaru 047-0193, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Okada
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamori
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.forename: Shuichi
authors.initials: S
authors.lastname: Onodera
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
authors.forename: Norio
authors.initials: N
authors.lastname: Shiomi
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts