title: Comparison of Recombinant Proteins from Schistosoma japonicum for Schistosomiasis Diagnosis.
abstract: The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant proteins with the potential to be diagnostic antigens were compared to the native soluble egg antigen preparation by enzyme-linked immunosorbent assay (ELISA). We evaluated the potentials of the recombinant proteins for discriminating active from prior infections, as well as the therapeutic efficacy of the established ELISA technique.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, People's Republic of China. yameijin@shvri.ac.cn
authors.forename: Ya-mei
authors.initials: YM
authors.lastname: Jin
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Zhou
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Yao-Jun
authors.initials: YJ
authors.lastname: Shi
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins