Flavones suppress type I IL-4 receptor signaling by down-regulating the expression of common gamma chain.
Immunoglobulin E (IgE) production is induced by interleukin (IL)-4 signaling mediated by type I IL-4 receptor (IL-4R) in B cells. We found that flavones inhibited IL-4-induced epsilon germline transcription which is essential for IgE class switching, and the phosphorylation of signal transducer and activator of transcription 6, janus kinase 3, and IL-4Ralpha, whereas IL-4 signaling mediated through type II IL-4R was unaffected by flavones. Furthermore, flavones reduced the expression of common gamma chain, a characteristic constituent subunit of type I IL-4R, suggesting that flavones suppress type I IL-4R signaling.
Yamashita
Shuya
S
Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamashita
Tatsunori
T
Yamada
Koji
K
Tachibana
Hirofumi
H
D047310:Apigenin / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D047309:Flavones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics / Q000378:metabolism; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics / Q000378:metabolism*; D015847:Interleukin-4 / Q000494:pharmacology; D053662:Interleukin-4 Receptor alpha Subunit / Q000378:metabolism; D053616:Janus Kinase 3 / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D034622:RNA Interference; D053658:Receptors, Interleukin-4, Type I / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050800:STAT6 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
D047309:Flavones; D005419:Flavonoids; D007139:Immunoglobulin epsilon-Chains; D053631:Interleukin Receptor Common gamma Subunit; D053662:Interleukin-4 Receptor alpha Subunit; D047495:PPAR gamma; D053658:Receptors, Interleukin-4, Type I; D050800:STAT6 Transcription Factor; D015847:Interleukin-4; C043561:chrysin; D047310:Apigenin; D053616:Janus Kinase 3