Multiple effects of amprenavir against Candida albicans.
Secreted aspartyl peptidases (Saps) are virulence attributes produced by Candida albicans that participate in multiple aspects of the fungal biology and pathogenesis. In the present paper, we have shown that amprenavir, a peptidase inhibitor used in HIV chemotherapy, inhibited Sap2 and growth of C. albicans and also promoted ultrastructural alterations. Esterase activity, sterol content, biofilm formation and the expression of surface mannose- and sialic acid-rich glycoconjugates were also reduced by amprenavir.
Braga-Silva
Lys A
LA
Laboratório de Estudos Integrados em Bioquímica Microbiana, Departamento de Microbiologia Geral, Centro de Ciências da Saúde, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Mogami
Sheijy S V
SS
Valle
Roberta S
RS
Silva-Neto
Ignácio D
ID
Santos
André L S
AL
D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors*; D018441:Biofilms / Q000187:drug effects; D001769:Blood / Q000382:microbiology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002177:Candidiasis / Q000382:microbiology; D002219:Carbamates / Q000494:pharmacology*; D003470:Culture Media; D005656:Fungal Proteins / Q000037:antagonists & inhibitors*; D016469:Fungemia / Q000382:microbiology; D005663:Furans; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D013449:Sulfonamides / Q000494:pharmacology*
D002219:Carbamates; D003470:Culture Media; D005656:Fungal Proteins; D005663:Furans; D017320:HIV Protease Inhibitors; D013449:Sulfonamides; C095108:amprenavir; D016282:Aspartic Acid Endopeptidases; C090753:SAP2 protein, Candida