Matrix metalloproteinase-9 reversibly affects the time course of NMDA-induced currents in cultured rat hippocampal neurons.
Matrix Metalloproteinase 9 (MMP-9) has been demonstrated to play a crucial role in maintenance of NMDA receptor-dependent LTP and in lateral mobility of these receptors. However, the effect of MMP-9 on NMDA receptor (NMDAR) functional properties is unknown. For this purpose we have investigated the impact of recombinant MMP-9 on the whole-cell NMDAR-mediated current responses in cultured hippocampal neurons. Treatment with MMP-9 induced a reversible acceleration of desensitization and deactivation kinetics but had no effect on current amplitude. Interestingly, phorbol ester, a PKC activator known to enhance NMDAR lateral mobility, induced kinetic changes of currents similar to those produced by MMP-9. In conclusion, our results show that MMP-9 reversibly modulates the NMDAR kinetics and raise a possibility that this modulation could be related to the lateral mobility of these receptors.
Gorkiewicz
Tomasz
T
Department of Biophysics, Laboratory of Neuroscience, Wroclaw Medical University, ul.Chalubinskiego 3, 50-367 Wroclaw, Poland.
Szczuraszek
Katarzyna
K
Wyrembek
Paulina
P
Michaluk
Piotr
P
Kaczmarek
Leszek
L
Mozrzymas
Jerzy W
JW
D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D020780:Matrix Metalloproteinase 9 / Q000494:pharmacology / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*
D016194:Receptors, N-Methyl-D-Aspartate; D020780:Matrix Metalloproteinase 9