Homeostatic alterations after IL-1beta microinjection into the nucleus accumbens of the rat.
The present study investigates the effects of direct administration of interleukin-1beta (IL-1beta) into the nucleus accumbens (NAcc) on homeostatic regulation. Short- and long-term food intakes (FI), water intakes (WI) and body temperature (BT) were measured before and after bilateral microinjection of IL-1beta (with or without paracetamol pretreatment) into the NAcc of Wistar rats, and the effects were compared with those found in vehicle treated control animals. In addition, blood glucose levels, along with a glucose tolerance test (GTT), and plasma concentrations of metabolic parameters, such as total cholesterol, triglycerides, HDL, LDL and uric acid were determined in cytokine treated and control rats. Short-term FI and WI were suppressed after intraaccumbens application of IL-1beta. A significant increase of BT was also observed after the cytokine microinjection. Pretreatment with paracetamol failed to influence the anorexigenic, adipsogenic, and pyrogenic effects of IL-1beta. A definite glucose intolerance of the cytokine treated animals and their pathologically elevated blood glucose levels became obvious in the acute GTT. Following IL-1beta microinjection, plasma levels of triglycerides, total cholesterol and LDL were found increased. Our present findings show that the NAcc is an important site of action of IL-1beta mediated processes in central homeostatic regulation.
Takács
Gábor
G
Institute of Physiology and Neurophysiology Research Group of the Hungarian Academy of Sciences, Pécs University, Medical School, Szigeti str. 12, H-7624 Pécs, Hungary. gabor.takacs@aok.pte.hu
Papp
Szilárd
S
Lukáts
Balázs
B
Szalay
Csaba
C
Nagy
Bernadett
B
Fotakos
Dimitrios
D
Karádi
Zoltán
Z
D000082:Acetaminophen / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001831:Body Temperature / Q000187:drug effects* / Q000502:physiology; D004326:Drinking / Q000187:drug effects* / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D006706:Homeostasis / Q000187:drug effects* / Q000502:physiology; D053583:Interleukin-1beta / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D008845:Microinjections; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014527:Uric Acid / Q000097:blood
D001786:Blood Glucose; D053583:Interleukin-1beta; D008055:Lipids; D014527:Uric Acid; D000082:Acetaminophen