Study on the effects of chloride depletion on photosystem II using different chloride depletion methods.
Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.
Jajoo
Anjana
A
School of Life Sciences, Devi Ahilya University, Indore, 452 001, M.P, India. anjanajajoo@hotmail.com
Mathur
Sonal
S
Mehta
Pooja
P
Yashioka
Miho
M
Yoshoika
Miho
M
Allakhverdiev
Suleyman I
SI
Yamamoto
Yasusi
Y
D002712:Chlorides / Q000302:isolation & purification / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism*; D018724:Spinacia oleracea / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
D002712:Chlorides; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D010100:Oxygen; D000077194:Chlorophyll A