title: Controlled dehydration of a biological sample using an alternative form of environmental SEM.
abstract: In this study a non-conductive biological sample is observed free of charging artefacts when placed on a cooled Peltier stage in the specimen chamber of an alternative form of the environmental scanning electron microscope, equipped with a specially designed hydration system. This system was used to create dynamically changing surrounding conditions leading to controlled dehydration of the sample enabling us to visualize the topographical structure of a rat tongue in the transition region between the liquid and the gas state of water in the microscope specimen chamber.
authors.affiliation: Institute of Scientific Instruments of ASCR, Královopolská 147, 61264, Czech Republic. vilem@isibrno.cz
authors.forename: V
authors.initials: V
authors.lastname: Nedĕla
mesh_terms: D000818:Animals; D003890:Desiccation / Q000295:instrumentation / Q000379:methods; D004777:Environment; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D044367:Phase Transition; D011312:Pressure; D051381:Rats; D013696:Temperature; D014059:Tongue / Q000737:chemistry / Q000648:ultrastructure*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water