[Expression and mutations of PIK3CA gene in hepatocellular carcinomas].
OBJECTIVE
To investigate the expression and mutation of PIK3CA gene in hepatocellular carcinomas (HCC).


METHODS
HCC samples and the corresponding adjacent tissues were collected from the surgical patients with pathologically verified diagnosis. The exons 1, 9 and 20 of PIK3CA gene were detected by PCR-SSCP and DNA sequencing. Immnohistochemistry was employed to test the expression of PIK3CA gene in these samples.


RESULTS
No mutation was found in exons 1, 9 or 20 of PIK3CA gene in the HCC tissue and the adjacent tissues by PCR-SSCP and DNA sequencing, while abnormal superimposed peaks were found on the sequence map of exon 9 in 25 cases of HCC tissue. Immunohistochemistry showed that expression of PIK3CA was higher in the HCC tissue than in the corresponding adjacent tissue (50.81% vs 14.75%).


CONCLUSIONS
PIK3CA gene mutation may exist in HCC in Guangxi, which can be associated with the development of HCC, but the ratio of hotspot mutations is low.
Yang
Ding-hua
DH
Department of Hepatobilliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. dhyang5810 @yahoo.com
Fu
Zhao-yin
ZY
Huang
Yu
Y
Zeng
Qi-yan
QY
Zhou
Jie
J
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D058534:Class I Phosphatidylinositol 3-Kinases; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human