[Primary study on mechanism of baicalin on the Th1/Th2 response in murine model of asthma].
OBJECTIVE
To study the mechanism of baicalin on the cytokines of Th1/Th2 in murine model of asthma.


METHODS
The murine model of asthma was induced by OVA. Different doses of baicalin were orally administered to the mice respectively. The spleen cells were cultured 3 days for the measurement of IFN-gamma, IL-4, IL-5 and IL-10 by ELISA. After 2 days of culture, the spleen cells were treated with Trizol for extraction of total RNA. The gene expressions of T-bet, GATA-3 and STAT-6 were analyzed by RT-PCR.


RESULTS
The treatment with baicalin obviously decreased the production of IL-4 and IL-5 and the gene expression of GATA-3, STAT-6, but increased the production of IL-10.


CONCLUSIONS
Baicalin may modulate the Th1/Th2 balance mainly by altering the gene expressions of GATA-3 and STAT-6 in vivo and increasing the production of IL-10.
Huang
Feng
F
College of Herbal Pharmacy, Jinan University, Guangzhou 510632, China. hftxyy@yahoo.com.cn
Tong
Xiao-yun
XY
Deng
Hua-ming
HM
Nie
Hong
H
Zhang
Rong-hua
RH
Cai
Yu
Y
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050800:STAT6 Transcription Factor / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D016207:Cytokines; D005419:Flavonoids; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; C038044:baicalin; D010047:Ovalbumin