title: [Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex].
abstract: The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liao
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Zhang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Yuan
authors.forename: Quan-Wei
authors.initials: QW
authors.lastname: Shi
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Yan
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Wang
authors.forename: Ke-Sheng
authors.initials: KS
authors.lastname: Dai
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D009154:Mutation; D010957:Plasmids; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex; D011994:Recombinant Proteins