Production of probiotic biomass (Lactobacillus casei) in goat milk whey: comparison of batch, continuous and fed-batch cultures.
This contribution examines the technical feasibility of producing high added value probiotic biomass from deproteinized and non-supplemented milk whey. The kinetics of growth of Lactobacillus casei in deproteinized goat milk whey was analyzed. Experiments in batch, continuous and fed-batch conditions were conducted in a 3 L fully instrumented bioreactor. Final substrate and biomass concentrations, yields and productivities are reported for different culture strategies. A kinetic analysis was conducted to characterize biomass production, product inhibition effects, and substrate consumption rates. Due to the strong product inhibition, fed-batch cultures at high biomass concentration rendered higher productivity (0.45 g L(-1) h(-1)) than batch and continuous cultures (0.11 g L(-1) h(-1)), complete lactose conversions (<1.0 g of lactose/L at the end of each fed-batch cycle), and a product with higher viable cell counts (2 x 10(10) cell/g of freeze-dried product). Based on our result, high-cell density fed-batch strategies are recommended for commercial production of probiotic L. casei biomass.
Aguirre-Ezkauriatza
E J
EJ
Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Ave. Eugenio Garza Sada 2501 sur. Monterrey, N.L., C.P. 64849, Mexico.
Aguilar-Yáñez
J M
JM
Ramírez-Medrano
A
A
Alvarez
M M
MM
D000818:Animals; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006041:Goats; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000254:growth & development*; D008892:Milk / Q000737:chemistry*; D019936:Probiotics