Fractionating soluble microbial products in the activated sludge process.
Soluble microbial products (SMP) are the pool of organic compounds originating from microbial growth and decay, and are usually the major component of the soluble organic matters in effluents from biological treatment processes. In this work, SMP in activated sludge were characterized, fractionized, and quantified using integrated chemical analysis and mathematical approach. The utilization-associated products (UAP) in SMP, produced in the substrate-utilization process, were found to be carbonaceous compounds with a molecular weight (MW) lower than 290 kDa which were quantified separately from biomass-associated products (BAP). The BAP were mainly cellular macromolecules with an MW in a range of 290-5000 kDa, and for the first time were further classified into the growth-associated BAP (GBAP) with an MW of 1000 kDa, which were produced in the microbial growth phase, and the endogeny-associated BAP (EBAP) with an MW of 4500 kDa, which were generated in the endogenous phase. Experimental and modeling results reveal that the UAP could be utilized by the activated sludge and that the BAP would accumulate in the system. The GBAP and EBAP had different formation rates from the hydrolysis of extracellular polymeric substances and distinct biodegradation kinetics. This study provides better understanding of SMP formation mechanisms and becomes useful for subsequent effluent treatment.
Ni
Bing-Jie
BJ
Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
Zeng
Raymond J
RJ
Fang
Fang
F
Xie
Wen-Ming
WM
Sheng
Guo-Ping
GP
Yu
Han-Qing
HQ
D018533:Biomass; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D008970:Molecular Weight; D009930:Organic Chemicals / Q000737:chemistry / Q000378:metabolism*; D010101:Oxygen Consumption; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D012995:Solubility; D014865:Waste Disposal, Fluid / Q000379:methods
D001704:Biopolymers; D009930:Organic Chemicals; D012722:Sewage