title: [Simultaneous determination of multi-organotin compounds in seawater by liquid-liquid extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry].
abstract: The hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was applied to the simultaneous determination of five organotin compounds (trimethyltin, dibutyltin, tributyltin, diphenyltin and triphenyltin) in seawater samples. Agilent TC-C18 column was used for the separation, the mobile phase of HPLC was CH3CN : H2O : CH3COOH = 65 : 23 : 12 (phi3), 0.05% TEA, and pH value was adjusted to 3.0 by diluent ammonia. The flow rate was 0.6 mL x min(-1). Five mixed organotin compounds in a mix standard solution from 100 to 0.5 microg x L(-1) were applied for the method assessment. The experimental results indicate that the correlation coefficient of calibration curves (R2) for each organotin compound was over 0.998 and the detection limits of the five organotin compounds were lower than 3 ng x L(-1). Different mixed organic solvents including dichloromethane or toluene were used for extraction of organotin and the extraction condition of organotin from seawater was optimized. The 100 mL seawater acidized by hydrochloric acid was extracted by 10 mL carbon dichloride (CH2 Cl2) with 2% tropolone for 10 min twice. Extracted organic solvents were mixed and blown to one drop by nitrogen with the rate of 1.7 mL x min(-1), then 1 mL acetonitrile was added to the drop for redissolving the organotin compounds. Finally, the mixed redissolution was filtered by 0.22 microm organic filter membrane before analysis. It was found that the only organotin compound in seawater was triphenyltin (TPHT) and the content was 53.2 ng x L(-1). The recoveries test from the standard addition for diphenyltin (DPHT), dibutyltin (DBT), tributyltin (TBT) and triphenyltin (TPHT) were over 80%. However, the recovery for trimethyltin (TMT) was relatively low and the value was 50%. The reason might be attributed to the decomposition or adsorption of those compounds during the extraction procedure. Further study on this subject is in progress.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. yzhhgm607@163.com
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Yu
authors.forename: Miao
authors.initials: M
authors.lastname: Jing
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Wang
authors.forename: Deng-Yun
authors.initials: DY
authors.lastname: Chen
authors.forename: Yan-Liang
authors.initials: YL
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry; D009930:Organic Chemicals; D009947:Organotin Compounds / Q000032:analysis*; D012623:Seawater; D012997:Solvents
chemical_list: D009930:Organic Chemicals; D009947:Organotin Compounds; D012997:Solvents; C056189:di-n-butyltin; C063612:diphenyltin; C030665:triphenyltin