[Metabonomic profiling of plasma metabolites in Wistar rats to study the effect of aging by means of GC/TOFMS-based techniques].
The global metabolite profiles of endogenous compounds of Wistar rats from 12 to 20 weeks old were investigated to take deep insight into and get better understanding of the pathogenesis of development and aging. Plasma from Wistar rats at 12, 14, 16, 18, and 20 weeks old were analyzed using GC/TOFMS. Multivariate data analysis was then used to process the metabonomic data which indicated excellent separation between different weeks and showed that the metabolic profiles of the samples changed with age, enabling age-related metabolic trajectories to be visualized. Decreased concentrations of citric acid, cis-aconitic acid, 9-(z)-hexadecenoic acid along with increased levels of hexanedioic acid, alpha-tocopherol, 3-indole propionic acid, etc contributed to the separation. Several major metabolic pathways were identified to be involved in metabolic regulation. This suggests that GC/TOFMS-based metabonomics is a powerful alternative approach to identifying potential biomarkers and investigating the physiological developments of aging and it is important to employ suitable age-match control group in metabonomic study of physiological monitoring, drug safety assessment, and disease diagnosis, etc.
Huang
Qing
Q
Jiangsu Institute for Food and Drug Control, Nanjing 210008, China.
Lu
Yi-Hong
YH
Wang
Guang-Ji
GJ
Wang
Xin-Wen
XW
Meng
Nan
N
Gao
Jie
J
Lu
Di
D
Yan
Xin
X
Zhang
Ying
Y
Liu
Lin-Sheng
LS
Hao
Gang
G
Yan
Bei
B
Gu
Sheng-Hua
SH
A
Ji-Ye
JY
D000156:Aconitic Acid / Q000097:blood; D000272:Adipates / Q000097:blood; D000375:Aging / Q000097:blood* / Q000502:physiology; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D019343:Citric Acid / Q000097:blood; D007211:Indoles / Q000097:blood; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D015999:Multivariate Analysis; D010169:Palmitic Acids / Q000097:blood; D011422:Propionates / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D024502:alpha-Tocopherol / Q000097:blood
D000272:Adipates; D015415:Biomarkers; D007211:Indoles; D010169:Palmitic Acids; D011422:Propionates; D019343:Citric Acid; C093821:hexadecenoic acid; C029900:adipic acid; D000156:Aconitic Acid; D024502:alpha-Tocopherol; C095446:3-(indol-3-yl)propionic acid