Milk versus caseinophosphopeptides added to fruit beverage: resistance and release from simulated gastrointestinal digestion.
The influence of simulated gastrointestinal digestion on caseinophosphopeptides (CPPs) formation in milk-based fruit beverage was evaluated, together with resistance of a pool of CPPs added to fruit beverage. In milk-based fruit beverage, four CPPs were identified that can be justified by their presence in raw milk or due to processing. When it was subjected to simulated gastrointestinal digestion, 10 CPPs were identified, and only 1 presented the cluster (SpSpSpEE) (3 phosphoseryl group followed by 2 glutamic acid residues), which corresponded to alpha(s2)-CN(1-19)4P. CPPs added to fruit beverage are resistant to simulated gastrointestinal digestion, and 16 CPPs were identified originating from the fragmentation of added CPPs, and with a greater presence of the cluster compared with CPPs originating from milk-based fruit beverage. This could justify the use of CPPs as functional ingredients, and offer a good alternative to milk-based fruit beverage for improving mineral bioavailability.
García-Nebot
María José
MJ
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Avda Vicente Andrés Estellés, 46100 Burjassot, Valencia, Spain.
Alegría
Amparo
A
Barberá
Reyes
R
Contreras
María del Mar
Mdel M
Recio
Isidra
I
D000595:Amino Acid Sequence; D000818:Animals; D001628:Beverages; D002364:Caseins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D005638:Fruit; D041981:Gastrointestinal Tract / Q000502:physiology; D006801:Humans; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010748:Phosphopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides