Non-apoptotic cell death induced by nutritional starvation in J774.1 mouse macrophage-like cell line.
The growth and amino acid utilization of a mouse macrophage-like cell line J774.1 was investigated in two different culture media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine, was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3 activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines, and DMEM is superior to RPMI1640 for long-term cell culture.
Thet
May Maw
MM
MPL, Division of Pharmacology, Meikai University School of Dentistry, Saitama 350-0283, Japan.
Yokote
Yoshiko
Y
Chu
Qing
Q
Wang
Qin-Tao
QT
Kishino
Kaori
K
Tamura
Nozomi
N
Shimada
Jun
J
Amano
Osamu
O
Kanda
Yumiko
Y
Kunii
Shiro
S
Takekawa
Fumihiro
F
Hatano
Hajime
H
Shimada
Chiyako
C
Hasegawa
Hideo
H
Sakagami
Hiroshi
H
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death; D002478:Cells, Cultured; D004789:Enzyme Activation; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D013217:Starvation
D000596:Amino Acids; D053148:Caspase 3