title: Carbondioxide pneumoperitoneum prevents postoperative adhesion formation in a rat cecal abrasion model.
abstract: OBJECTIVE
  We aimed to investigate the role of the carbon-dioxide (CO(2)) pneumoperitoneum on the prevention of postoperative adhesion formation.
  
  
  METHODS
  Thirty adult male Sprague-Dawley rats (250-350 g) were used for this study. The rats were randomly divided into three groups; in the sham group (n = 10) only, laparotomy was made, in group 2 (n = 10), a pneumoperitoneum was applied for a half-hour duration, followed by laparatomy, and in group 3 (n = 10), pneumoperitoenum time was 2 hours and after a pneumoperitoneum laparotomy was made. A cecal abrasion model was studied for making an adhesion formation. On day 14, all rats were sacrificed and adhesions were scored. Tissue samples from adhesions and peritonea and the cecum wall were examined, both pathologically and biochemically, for tissue hydroxyproline content.
  
  
  RESULTS
  There was a statistically significant difference between the control and pneumoperitoneum groups, regarding adhesion numbers and grades (P < 0.001). Cecal tissue hydroxypyroline content level was lower in group 1, and statistically significant differences were observed between groups 1, 2, and 3 regarding hydroxyproline content (P < 0.001). There was a statistically significant difference between the control and pneumoperitoneum groups regarding inflammation (P = 0.002).
  
  
  CONCLUSIONS
  Pretreatment with the CO(2) pneumoperitoneum had a prophylactic effect on postoperative adhesion formation in this study.
authors.affiliation: Gastroenterologic Surgery, Yuksek Ihtisas Hospital, Ankara, Turkey. cankece@gmail.com
authors.forename: Can
authors.initials: C
authors.lastname: Kece
authors.forename: Murat
authors.initials: M
authors.lastname: Ulas
authors.forename: Ilter
authors.initials: I
authors.lastname: Ozer
authors.forename: Ummuhani
authors.initials: U
authors.lastname: Ozel
authors.forename: Ayse
authors.initials: A
authors.lastname: Bilgehan
authors.forename: Gulden
authors.initials: G
authors.lastname: Aydog
authors.forename: Tahsin
authors.initials: T
authors.lastname: Dalgic
authors.forename: Erkan
authors.initials: E
authors.lastname: Oymaci
authors.forename: Birol
authors.initials: B
authors.lastname: Bostanci
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage; D002429:Cecal Diseases / Q000517:prevention & control*; D008297:Male; D010532:Peritoneal Diseases / Q000517:prevention & control*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000517:prevention & control
chemical_list: D002245:Carbon Dioxide