Covalent bond between ligand and receptor required for efficient activation in rhodopsin.
Rhodopsin is an extensively studied member of the G protein-coupled receptors (GPCRs). Although rhodopsin shares many features with the other GPCRs, it exhibits unique features as a photoreceptor molecule. A hallmark in the molecular structure of rhodopsin is the covalently bound chromophore that regulates the activity of the receptor acting as an agonist or inverse agonist. Here we show the pivotal role of the covalent bond between the retinal chromophore and the lysine residue at position 296 in the activation pathway of bovine rhodopsin, by use of a rhodopsin mutant K296G reconstituted with retinylidene Schiff bases. Our results show that photoreceptive functions of rhodopsin, such as regiospecific photoisomerization of the ligand, and its quantum yield were not affected by the absence of the covalent bond, whereas the activation mechanism triggered by photoisomerization of the retinal was severely affected. Furthermore, our results show that an active state similar to the Meta-II intermediate of wild-type rhodopsin did not form in the bleaching process of this mutant, although it exhibited relatively weak G protein activity after light irradiation because of an increased basal activity of the receptor. We propose that the covalent bond is required for transmitting structural changes from the photoisomerized agonist to the receptor and that the covalent bond forcibly keeps the low affinity agonist in the receptor, resulting in a more efficient G protein activation.
Matsuyama
Take
T
Department of Biophysics, Graduate School of Science and CREST-JST, Kyoto University, Kyoto 606-8502, Japan.
Yamashita
Takahiro
T
Imai
Hiroo
H
Shichida
Yoshinori
Y
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007536:Isomerism; D007668:Kidney / Q000166:cytology; D008024:Ligands; D008239:Lysine / Q000378:metabolism; D016296:Mutagenesis; D010775:Photic Stimulation; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D012172:Retinaldehyde / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012545:Schiff Bases; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship; D013696:Temperature; D014785:Vision, Ocular / Q000502:physiology*
D008024:Ligands; D012545:Schiff Bases; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D008239:Lysine; D012172:Retinaldehyde