title: Characterization of the human neutrophil alloantigen-3a.
abstract: Transfusion-related acute lung injury (TRALI) is a frequent cause of transfusion-associated morbidity and mortality. Severe TRALI is often due to antibodies in blood components directed against the human neutrophil alloantigen-3a (HNA-3a). We show here that the HNA-3a antigen arises from a nucleotide polymorphism in the choline transporter-like protein-2 gene (SLC44A2), with the resulting variation at amino acid position 154 determining the reactivity of the protein with HNA-3a-specific antibodies; the variant with an arginine at this position, rather than a glutamine, constitutes the HNA-3a antigen. The molecular identification of this antigen should facilitate the development of assays for blood donor screening to lower the risk of TRALI.
authors.affiliation: Institut für Immunologie und Transfusionsmedizin, Ernst Moritz-Arndt-Universität Greifswald, Greifswald, Germany. greinach@uni-greifswald.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Greinacher
authors.forename: Jan
authors.initials: J
authors.lastname: Wesche
authors.forename: Elke
authors.initials: E
authors.lastname: Hammer
authors.forename: Birgitt
authors.initials: B
authors.lastname: Fürll
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.forename: Angelika
authors.initials: A
authors.lastname: Reil
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bux
mesh_terms: D055371:Acute Lung Injury / Q000235:genetics* / Q000276:immunology; D000372:Agglutination Tests; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D065227:Transfusion Reaction
chemical_list: C518134:HNA-3a antigen, human; D007519:Isoantigens; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C484524:SLC44A2 protein, human