High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry.
Natural polyisoprene is a biopolymer consisting of isoprene units (C(5)H(8)) that is used commercially in household, medical, and industrial materials. For the management of natural polyisoprene production, the selection of high-yield polyisoprene-producing trees, and an understanding of polyisoprene biosynthesis, a high-throughput and highly sensitive screening method for the quantification of polyisoprene is required. In this study, we examined pyrolysates from polyisoprenes, polyprenols, carotenoids, ubiquinone (CoQ-10), and sterols by pyrolysis gas chromatography/mass spectrometry (PyGC/MS) and determined that the amounts of isoprene and limonene released from polyprenols and polyisoprenes were dependent upon their molecular weights. Based on these results, we developed a relative quantification method for polyisoprene in leaves by direct analysis of 1 mg of leaves using PyGC/MS. This novel quantification method eliminated extraction steps and can be used in the measurement of polyisoprene contents in Eucommia ulmoides and Hevea brasiliensis.
Takeno
Shinya
S
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Bamba
Takeshi
T
Nakazawa
Yoshihisa
Y
Fukusaki
Eiichiro
E
Okazawa
Atsushi
A
Kobayashi
Akio
A
D016227:Benzoquinones / Q000737:chemistry; D002070:Butadienes / Q000032:analysis / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D053138:Cyclohexenes / Q000032:analysis / Q000737:chemistry; D031283:Eucommiaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D045782:Hemiterpenes / Q000032:analysis* / Q000737:chemistry*; D028482:Hevea / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D007840:Latex / Q000032:analysis* / Q000737:chemistry*; D000077222:Limonene; D008970:Molecular Weight; D010420:Pentanes / Q000032:analysis / Q000737:chemistry; D013261:Sterols / Q000737:chemistry; D013729:Terpenes / Q000032:analysis / Q000737:chemistry
D016227:Benzoquinones; D002070:Butadienes; D053138:Cyclohexenes; D045782:Hemiterpenes; D007840:Latex; D010420:Pentanes; D013261:Sterols; D013729:Terpenes; C005059:isoprene; D002734:Chlorophyll; D002338:Carotenoids; C004532:quinone; C455100:1,4-polyisoprene; D000077222:Limonene