Anti-inflammatory principles from the stem and root barks of Citrus medica.
Bioassay-guided investigation of the anti-inflammatory principles from the stem and root barks of Citrus medica L. var. sarcodactylis SWINGLE has led to the isolation of a new coumarin, namely citrumedin-B (1) and thirty known compounds. The anti-inflammatory components were xanthyletin (2), nordentatin (3), atalantoflavon (4) and lonchocarpol A (5) which displayed potent nitric oxide (NO)-reducing activity in microglial cells. The structure of this new compound was completely elucidated using a combination of 2D NMR techniques (correlation spectroscopy (COSY), nuclear Overhauser effect spectroscopy (NOESY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC)) and HR-electrospray ionization (ESI)-MS analyses. The known compounds were identified by comparison of their spectroscopic and physical data with those reported in the literature. These results can be inferred from the treatment of allergic response and inflammatory properties of Citrus medica L. var. sarcodactylis SWINGLE in traditional Chinese medicine.
Chan
Yu-Yi
YY
Department of Biotechnology, Southern Taiwan University of Technology, Taiwan, Republic of China.
Li
Chan-Hao
CH
Shen
Yuh-Chiang
YC
Wu
Tian-Shung
TS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002957:Citrus / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017628:Microglia / Q000166:cytology; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D003374:Coumarins; D009569:Nitric Oxide