The copepod Tigriopus japonicus genomic DNA information (574Mb) and molecular anatomy.
The intertidal copepod, Tigriopus japonicus, has been recognized as a promising model species for marine environmental genomics. To obtain extensive genomic DNA sequences from this species, we sequenced genomic DNA from adult copepods using genomic sequencers GS-FLX and GS-FLX-Titanium and attained 1,914,995 reads (average read length 299.8 bp) including 574.2 Mb of genomic DNA information. After subjecting them to assembly, we acquired 193,642 contigs (total contigs length 129.7 Mb), and finally were able to obtain 10,894 unigenes (E-value>0.1; length>200 bp) containing 33,081,455 bp after a nonredundant (NR) blast search. In this paper, we summarize the genomic DNA sequences of T. japonicus and discuss its potential use in environmental genomics and ecotoxicological studies for uncovering mechanisms of environmental stresses and chemical toxicities to marine crustaceans.
Lee
Jae-Seong
JS
Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea. jslee2@hanyang.ac.kr
Rhee
Jae-Sung
JS
Kim
Ryeo-Ok
RO
Hwang
Dae-Sik
DS
Han
Jeonghoon
J
Choi
Beom-Soon
BS
Park
Gyung Soo
GS
Kim
Il-Chan
IC
Park
Heum Gi
HG
Lee
Young-Mi
YM
D000818:Animals; D033342:Copepoda / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D054750:Ecotoxicology; D004784:Environmental Monitoring; D005796:Genes; D017422:Sequence Analysis, DNA
D004247:DNA