[Methylation status of CpG islands in secreted frizzled-related protein gene promoter region of malignant hematopoietic cell lines].
To investigate the methylation status of CpG islands of the secreted frizzled-related protein (SFRP) gene promoter region in malignant hematopoietic cell lines, and to explore the possible relationship of CpG abnormal methylation status with pathogenic mechanism of hematologic malignancies. Methylation-specific PCR was used to detect the status of SFRP gene promoter region in nine malignant hematopoietic cell lines and peripheral blood mononuclear cells from healthy people. The results indicated that hypermethylation of 2 genes coding for SFRP1 and 2 were present in nine malignant hematopoietic cell lines, however, methylation and unmethylation of SFRP4 were both detected in CA46, HL60 and U937 cell lines, and SFRP5 in U266 as well. None of the normal mononuclear cells showed methylation of SFRP 1-5 genes. It is concluded that the hypermethylation of SFRP genes is related to the evolution of malignant hematopoiesis. Methylation of SFRP genes may serve as potential independent biomarkers for early detection of hematologic malignancies.
Xu
Cheng-Bo
CB
Department of Hematology, Union Hospital, Fujian Medical University; Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.
Shen
Jian-Zhen
JZ
Shen
Song-Fei
SF
Fu
Hai-Ying
HY
Wu
Xue-Mei
XM
Wu
Dan-Sen
DS
Zhu
Yi-Fang
YF
Chen
Lu
L
D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D000094742:Secreted Frizzled-Related Proteins
D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C117636:SFRP1 protein, human; D000094742:Secreted Frizzled-Related Proteins; C109618:SFRP2 protein, human; C118723:SFRP4 protein, human