[Studies on extraction process of the main saponin constituents from the stem bark of Kalopanax septemlobus in Guangxi].
Using orthogonal experiment design, the total saponin constituents were obtained by refluxing extraction with alcohol and separated by macroporous adsorption resin and n-Butyl alcohol from the stem bark of Kalopanax septemlobus. According to the purity analysis and the yield, the extraction process was optimized. The results showed that the main saponin constituents were gained with a yield of 1.32% by using macroporous adsorption resin but 1.05% by using n-Butyl alcohol. The former was more efficient than the latter on both yield and color. The optimal process with isolation by macroporous adsorption resin is cheap, simple and practical.
Yang
Yue
Y
Department of Natural Medicinal, Guilin Medical College, Guilin 541004, China. yangyue1951@glme.edu.cn
Yang
Xin-ping
XP
Liu
Xiao-fu
XF
Jiang
Xiao-jun
XJ
D020001:1-Butanol / Q000737:chemistry; D000327:Adsorption; D002855:Chromatography, Thin Layer; D000431:Ethanol / Q000737:chemistry; D036882:Kalopanax / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*
D012117:Resins, Synthetic; D012503:Saponins; D000431:Ethanol; D020001:1-Butanol