title: Efficacy of an oral live vaccine for veterinary use against pseudotuberculosis.
abstract: Pseudotuberculosis, an infection caused by the ubiquitous enteropathogenic bacterium Yersinia pseudotuberculosis, is a recurrent veterinary problem in livestock and zoo animals. The only vaccine currently available in zoos is Pseudovac (a mixture of killed strains of various serotypes), but its efficacy is not well established. We show here that Pseudovac does not protect guinea pigs against a severe Y. pseudotuberculosis infection. We thus evaluated the possibility of using a live attenuated Y. pseudotuberculosis strain (IP32680) as an oral vaccine against animal pseudotuberculosis. We report that IP32680 is avirulent for guinea pigs and induces a strong IgG response against various serotypes of Y. pseudotuberculosis. One and two oral inoculations of IP32680 provided 50% and 83% protection, respectively against a severe infection with a highly pathogenic strain. The avirulent Y. pseudotuberculosis IP32680 is therefore much more protective than Pseudovac and may represent a valuable oral vaccine against pseudotuberculosis in zoo animals.
authors.affiliation: Muséum de Besançon, La Citadelle, 25000 Besançon, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Quintard
authors.forename: Thierry
authors.initials: T
authors.lastname: Petit
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ruvoen
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Carniel
authors.forename: Christian E
authors.initials: CE
authors.lastname: Demeure
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000097:blood*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000276:immunology* / Q000472:pathogenicity; D015012:Yersinia pseudotuberculosis Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated