title: Challenges for biomarker discovery in body fluids using SELDI-TOF-MS.
abstract: Protein profiling using SELDI-TOF-MS has gained over the past few years an increasing interest in the field of biomarker discovery. The technology presents great potential if some parameters, such as sample handling, SELDI settings, and data analysis, are strictly controlled. Practical considerations to set up a robust and sensitive strategy for biomarker discovery are presented. This paper also reviews biological fluids generally available including a description of their peculiar properties and the preanalytical challenges inherent to sample collection and storage. Finally, some new insights for biomarker identification and validation challenges are provided.
authors.affiliation: Laboratory of Clinical Chemistry, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium.
authors.forename: Muriel
authors.initials: M
authors.lastname: De Bock
authors.forename: Dominique
authors.initials: D
authors.lastname: de Seny
authors.forename: Marie-Alice
authors.initials: MA
authors.lastname: Meuwis
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Chapelle
authors.forename: Edouard
authors.initials: E
authors.lastname: Louis
authors.forename: Michel
authors.initials: M
authors.lastname: Malaise
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Merville
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D015415:Biomarkers