High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors.
An acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 was found to be suitable for the production of high amounts of citric acid in long-term repeated-batch cultures. When 40% of fresh replaced medium was fed, this strain produced 154 g l(-1), on average, which corresponded to a 0.78 g g(-1) yield and a productivity of 1.05 g l(-1) h(-1). The activity of the culture remained stable for more than 1,650 h, i.e., 16 cycles of the repeated-batch bioreactors.
Rywińska
Anita
A
Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, Wroclaw, Poland. Anita.Rywinska@up.wroc.pl
Rymowicz
Waldemar
W
D000085:Acetates / Q000378:metabolism; D019149:Bioreactors; D019343:Citric Acid / Q000378:metabolism*; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism*; D007218:Industrial Microbiology; D009154:Mutation; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
D000085:Acetates; D003470:Culture Media; D019343:Citric Acid; D005990:Glycerol