Understanding protein phosphorylation on a systems level.
Protein kinase phosphorylation is central to the regulation and control of protein and cellular function. Over the past decade, the development of many high-throughput approaches has revolutionized the understanding of protein phosphorylation and allowed rapid and unbiased surveys of phosphoproteins and phosphorylation events. In addition to this technological advancement, there have also been computational improvements; recent studies on network models of protein phosphorylation have provided many insights into the cellular processes and pathways regulated by phosphorylation. This article gives an overview of experimental and computational techniques for identifying and analyzing protein phosphorylation on a systems level.
Lin
Jimmy
J
Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Xie
Zhi
Z
Zhu
Heng
H
Qian
Jiang
J
D000818:Animals; D020134:Catalytic Domain; D032882:Comprehension; D019295:Computational Biology / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000502:physiology; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism* / Q000502:physiology; D040901:Proteomics / Q000379:methods
D010750:Phosphoproteins; D011494:Protein Kinases