title: Platelet-rich plasma and 1,25(OH)2 vitamin D3 synergistically stimulate osteogenic differentiation of adult human mesenchymal stem cells.
abstract: Mesenchymal stem cell proliferation and differentiation are regulated by growth factors and systemic hormones in a concerted manner. We have examined the effects of platelet-rich plasma (PRP) and its interaction with 1,25(OH)(2) vitamin D(3) [1,25(OH)(2)D(3)] on the differentiation and proliferation of human mesenchymal stem cells (hMSCs). PRP stimulated cell proliferation in a manner inversely proportional to the dose. Exposure to PRP inhibited hMSC osteogenic differentiation. Co-treatment with PRP and 1,25(OH)(2)D(3), however, synergistically stimulated osteogenic differentiation. Serial co-treatment with PRP and 1,25(OH)(2)D(3) showed that this synergistic effect was due to the modulation of a 1,25(OH)(2)D(3)-induced differentiation by PRP rather than vice versa.
authors.affiliation: Department of Orthopaedics, The Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Weitao
authors.initials: W
authors.lastname: Jia
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000328:Adult; D002117:Calcitriol / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D002117:Calcitriol