[A rapid colorimetric assay for viability of osteosarcoma cells after chemotherapy].
OBJECTIVE
To introduce a rapid colorimetric method for assessing the viability of osteosarcoma cells after chemotherapy.


METHODS
Colorimetric assay and automatic microplate scanning spectrophotometer were used for assaying the viability of osteosarcoma cells.


RESULTS
Close correlation was found between the absorbance at 570 nm of the formazan products and the number of viable osteosarcoma cells.


CONCLUSIONS
An effective, sensitive and convenient colorimetric assay has been established to assess the survival of osteosarcoma cells following chemotherapy.
Chen
Qiang
Q
Department of Spinal and Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. cqnfyy@yahoo.com.cn
Zhang
Jin-hua
JH
Chen
Jian-ting
JT
Shi
Zhan-jun
ZJ
Deng
Yong-jian
YJ
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry / Q000379:methods; D005562:Formazans; D006801:Humans; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology*; D012680:Sensitivity and Specificity; D013778:Tetrazolium Salts
D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan