Lack of heterogeneity of HPV16 E7 sequence compared with HPV31 and HPV73 may be related to its unique carcinogenic properties.
To assess the role of human papillomavirus virus (HPV) genetics in cervical lesions, we sequenced the E7 gene of HPV16, 31, or 73 from singly infected women who (1) cleared the infection quickly, (2) had type-specific persistent infection, or (3) progressed to CIN2 or worse lesions. Four of the 296 HPV16 E7 nucleotides were variable, compared with 7 of 296 for HPV31 E7 and 4 of 296 for HPV73 E7. While most of the polymorphisms in HPV31 and -73 resulted in non-synonymous amino acid changes, the polymorphisms in the HPV16 E7 resulted in synonymous changes. The lack of heterogeneity of HPV16 E7 suggests high evolutionary purifying selection that might be related to the unique carcinogenicity of HPV16.
Safaeian
Mahboobeh
M
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, USA. safaeianm@mail.nih.gov
van Doorslaer
Koenraad
K
Schiffman
Mark
M
Chen
Zigui
Z
Rodriguez
Ana Cecilia
AC
Herrero
Rolando
R
Hildesheim
Allan
A
Burk
Robert D
RD
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008875:Middle Aged; D020125:Mutation, Missense; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D050725:Papillomavirus E7 Proteins / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D017354:Point Mutation; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
C502189:E7 protein, Human papillomavirus type 31; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16