title: Specific IgG responses to recombinant antigen B and em18 in cystic and alveolar echinococcosis in china.
abstract: An understanding of the correlation of the specific antibody responses and the disease phase is essential in evaluating diagnostic values of immunological tests in human echinococcosis. In this study, 422 echinococcosis patients diagnosed by ultrasonography, including 246 with cystic echinococcosis (CE), 173 with alveolar echinococcosis (AE), and 3 with dual infection, were tested for specific IgG in sera against recombinant AgB (rAgB) and recombinant Em18 (rEm18) in an enzyme-linked immunosorbent assay. As a result, rAgB-specific antibody was detected in 77.6% of CE and 86.1% of AE patients, while rEm18-specific antibody was present in 28.9% of CE and 87.3% of AE patients. Additionally, all three patients with dual infection exhibited specific antibodies responding to rAgB and rEm18. Further analysis revealed that rAgB-specific antibody was elevated in a significantly greater proportion (87.3%) of CE patients with cysts at active or transitional stages (CE1, CE2, or CE3), compared to 54.8% of other patients with cysts at an early or an inactive stage (CL or CE4 or CE5). Furthermore, rAgB-specific antibody was detected in 95.6% of CE2 cases, which was statistically greater than that (73.7%) in CE1 patients. Although rEm18-specific antibody was elevated in 28.9% of CE patients, the positive reaction was much weaker in CE than in AE cases. Serum levels and concentrations of rEm18-specific antibody were further indicated to be strongly disease phase correlated in AE patients, with positive rates of 97.4% in cases with alveolar lesions containing central necrosis and 66.7% in patients with early alveolar lesions that measured </=5 cm.
authors.affiliation: Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Chengdu 610041, Sichuan Province, China. litiaoying@sina.com
authors.forename: Tiaoying
authors.initials: T
authors.lastname: Li
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Xingwang
authors.initials: X
authors.lastname: Chen
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Jiamin
authors.initials: J
authors.lastname: Qiu
authors.forename: Ning
authors.initials: N
authors.lastname: Xiao
authors.forename: Dongchuan
authors.initials: D
authors.lastname: Qiu
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yanagida
authors.forename: Philip S
authors.initials: PS
authors.lastname: Craig
mesh_terms: D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D002681:China; D004443:Echinococcosis / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D015801:Helminth Proteins; D007074:Immunoglobulin G; D008074:Lipoproteins; D011994:Recombinant Proteins; C089273:antigen B, Echinococcus