Characterization of cytoplasmic motifs important in rhesus rhadinovirus gB processing and trafficking.
Rhesus monkey rhadinovirus (RRV) is highly related to Kaposi's sarcoma-associated herpesvirus (KSHV), a human gamma-herpesvirus etiologically-linked with several cancers. Glycoprotein B (gB) homologues are encoded by all herpesviruses and play a role in virus attachment, entry, and in egress. We have found that RRV gB, like KSHV gB, is cleaved at a consensus furin cleavage site and is modified by both N-linked and O-linked glycosylation. Mutagenesis of three tyrosine- based trafficking motifs, a diacidic tyrosine motif, and a di-lucine motif in the cytoplasmic region revealed a role for these sequences in both ER export and endocytosis from the plasma membrane. These experiments provide a basis for further experiments looking at gB incorporation and role in gamma-herpesvirus assembly and egress.
Lang
Sabine M
SM
Department Of Pathology, Yale University School of Medicine, P.O. Box 208023, New Haven, CT 06520, USA.
Means
Robert E
RE
D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004705:Endocytosis; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology; D006031:Glycosylation; D006566:Herpesviridae Infections / Q000821:virology; D008253:Macaca mulatta / Q000821:virology; D016297:Mutagenesis, Site-Directed; D011499:Protein Processing, Post-Translational; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D018144:Rhadinovirus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology; D053585:Virus Attachment
D006023:Glycoproteins; D014759:Viral Envelope Proteins