Titanium-coated silica spheres prepared by self-assembly technique for use as HPLC packing.
Titania-coated silica spheres were prepared by a layer-by-layer self-assembly technique for use as a high-performance liquid chromatography packing. This packing has a high surface area of 202.1 m(2)/g, a large pore volume of 0.36 cm(3)/g, and a pore diameter of 7.0 nm. Furthermore, the packing particles exhibit narrow pore size distribution and good pore structure. The chromatographic behavior of the packing was studied under both normal and reversed-phase conditions. Low column pressures were observed. Aromatic isomeric compounds were well separated on the TiO(2)/SiO(2) column under normal phase conditions; pyridine and aniline derivatives were separated on the octadecyl-bonded TiO(2)/SiO(2) (ODT) column, respectively, under reversed-phase conditions, and highly symmetrical peaks were obtained for 15 basic compounds. The chemical stability of the stationary phase was tested with sodium phosphate solution (10 mmol/L, pH = 10) at 25 degrees C and potassium phosphate solution (50 mmol/L, pH = 10) at 50 degrees C.
Ge
Jin
J
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Zhao
Liang
L
Chen
Li-Ren
LR
Shi
Yan-Ping
YP
D000814:Aniline Compounds / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006841:Hydrocarbons, Aromatic / Q000302:isolation & purification*; D010316:Particle Size; D016062:Porosity; D011725:Pyridines / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D000814:Aniline Compounds; D006841:Hydrocarbons, Aromatic; D011725:Pyridines; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium; C023666:pyridine; C023650:aniline