title: Inhibitory effect of Angelicae Tenuissimae Radix on expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells.
abstract: OBJECTIVE
  Angelicae Tenuissimae Radix has traditionally been used for treating headache, flu-like symptoms, limb-ache and disability, and even for treating toothache. We investigated the anti-inflammatory and analgesic effect of Angelicae Tenuissimae Radix on lipopolysaccharide-induced inflammation.
  
  
  METHODS
  For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2) immunoassay and nitric oxide detection in mouse BV2 microglial cells were performed.
  
  
  RESULTS
  Angelicae Tenuissimae Radix suppressed prostaglandin E(2) synthesis and nitric oxide production by inhibiting the lipopolysaccharide-induced expressions of cyclooxygenase-2 and inducible nitric oxide synthase messenger RNA in mouse BV2 microglial cells.
  
  
  CONCLUSIONS
  The present study indicates that Angelicae Tenuissimae Radix can be applied as a valuable treatment for brain inflammation and headache.
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Suh-Ha
authors.initials: SH
authors.lastname: Lee
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Choi
authors.forename: Hocheol
authors.initials: H
authors.lastname: Kim
authors.forename: Hyejung
authors.initials: H
authors.lastname: Lee
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Sung
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Chang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Mal-Soon
authors.initials: MS
authors.lastname: Shin
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D029969:Angelica; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007118:Immunoassay; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C022616:thiazolyl blue