title: Mercuric mercaptide of penicillenic acid, a novel hapten for relevant immunoassay, synthesized from penicillin.
abstract: The synthesis of mercuric mercaptide of penicillenic acid (MMPA) has been the basis for detection of penicillin for nearly 40 years (J. Pharm. Pharmacol., 1972, 24, 790; Chinese Pharmacopoeia Ed. II, 1995). Herein, experiments were performed on: (1) synthesis of MMPA used as a novel mercuric hapten, (2) preparation of mercuric antigen of MMPA-BSA, (3) production of antibodies by rabbits immunized with the antigen, and (4) properties of the antibodies studied by ELISA. The results show that: (1) the antigen is safe for immunized animals; (2) high titer antibodies against MMPA are obtained implying good immunogenicity of the antigen; (3) antisera show slightly higher affinity to OVA-GHS-HgCl than OVA-GSH, indicating weak specific affinity of antisera against mercuric ion. Even the weak specific affinity, the hapten and its antigen have potential uses in immunoassays of mercuric ion in environment and food samples, because of easy chemical selective conversion from mercuric ion to MMPA and complete decomposition of un-reacted penicillin in acidic solution.
authors.affiliation: Laboratory of Analytical Biochemistry & Bio-separation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.forename: Xi
authors.initials: X
authors.lastname: Tao
authors.forename: Wu
authors.initials: W
authors.lastname: Xu
authors.forename: Liu-Yin
authors.initials: LY
authors.lastname: Fan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yue-E
authors.initials: YE
authors.lastname: Zhi
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
authors.forename: Cheng-Xi
authors.initials: CX
authors.lastname: Cao
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000918:Antibody Specificity; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009941:Organomercury Compounds / Q000138:chemical synthesis / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010406:Penicillins / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000906:Antibodies; D006241:Haptens; D009941:Organomercury Compounds; D010406:Penicillins; C547708:mercuric mercaptide of penicillenic acid; D012710:Serum Albumin, Bovine; D010047:Ovalbumin