[Effects of calcium way of Schwann cells on damage of peripheral nerve induced by acrylamide].
OBJECTIVE
To explore the mechanism of Schwann cells on damage of peripheral nerve injured by acrylamide.


METHODS
(1) Animal model: the neurobehavior and pathologic changes within exposure and recovery period were observed. The changes of Synapsin-I and S-100beta were measured by immunohistochemical method. (2) Cell mode: in this study, primary culture of Schwann cells and dorsal root ganglion (DRG) neurons and the co-culture of the two cells were established before the test. The toxicity of ACR on nerve cells was measured by MTT. Intracellular calcium concentration was examined for the protection of Schwann cells.


RESULTS
Toxic symptoms of female were occurred obviously at cumulative dose (300-400 mg/kg). The reparative changes were found significantly after recovering 4 to 5 weeks. The signals of S-100beta were weakened after exposure. But within the extended recovery period, its positive signals were gradually increased in consistent with the decrease of toxic symptoms. The changes of Synapsin-I were consistent with that of S-100beta. Compared with non co-cultured neurons, co-cultured neurons have significant lower intracellular calcium concentration injured by acrylamide.


CONCLUSIONS
The calcium channel of Schwann cells effects on the repair of peripheral nerve which is injured by acrylamide and Synapsin-I may be a key role site.
Xiao
Jingwei
J
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. xjw7811@126.com
Niu
Kailong
K
Meng
Huilin
H
Cui
Tao
T
Li
Zhongsheng
Z
Li
Bin
B
D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D059348:Peripheral Nerve Injuries; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D016704:Synapsins / Q000378:metabolism
D015220:Calcium Channels; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D016704:Synapsins; D020106:Acrylamide; D002118:Calcium