[Optimization of methods for DNA extraction from objects of forensic medical examination].
An optimized method for DNA extraction from materials available for forensic medical examination is proposed based on partial combination of two procedures, phenol-chloroform extraction and sorption. Such a combination provided a basis for the development of a protocol making use of the advantages of both procedures and free of drawbacks of either of them. This relatively simple and rapid method uses no reagents having adverse effect on human organism. Also, it excludes the use of substances interfering with polymerase chain reaction (PCR) and thereby reduces the risk of DNA loss. This makes it suitable for the work with objects having very low DNA content. The DNA specimens obtained with the help of the proposed technique are fit for prolonged storage.
Ivanov
P L
PL
Kaganova
N L
NL
D004247:DNA / Q000302:isolation & purification*; D053803:Forensic Genetics / Q000379:methods* / Q000592:standards; D006801:Humans; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards
D004247:DNA