title: Nanoparticle formation of organic compounds with retained biological activity.
abstract: Many pharmaceuticals are formulated as powders to aid drug delivery. A major problem is how to produce powders having high purity, controlled morphology, and retained bioactivity. We demonstrate the use of supercritical carbon dioxide as an antisolvent for meeting this need for two model drug systems, quercetin, a sparingly soluble antioxidant, and short interfering RNA (siRNA), which can silence genes. In both cases we achieve retention of bioactivity as well as a narrow particle size distribution in which the particles are free of impurities.
authors.affiliation: Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, California 94305-5080, USA.
authors.forename: Gunilla B
authors.initials: GB
authors.lastname: Jacobson
authors.forename: Rajesh
authors.initials: R
authors.lastname: Shinde
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: McCullough
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Cheng
authors.forename: Adam
authors.initials: A
authors.lastname: Creasman
authors.forename: Amanuel
authors.initials: A
authors.lastname: Beyene
authors.forename: Robyn P
authors.initials: RP
authors.lastname: Hickerson
authors.forename: Can
authors.initials: C
authors.lastname: Quan
authors.forename: Charlotta
authors.initials: C
authors.lastname: Turner
authors.forename: Roger L
authors.initials: RL
authors.lastname: Kaspar
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Contag
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D053758:Nanoparticles; D009930:Organic Chemicals; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011208:Powders
chemical_list: D009930:Organic Chemicals; D004364:Pharmaceutical Preparations; D011208:Powders; D002245:Carbon Dioxide