title: Spectroscopic investigation of the interaction of p-benzoquinone with casein in the solid state.
abstract: The reaction between p-benzoquinone (PBQ) and casein (protein) in the solid state was studied. After reaction with protein, the FT-IR spectra showed a new band at 1215 cm(-1), with an intense sign attributed for casein/PBQ product. An optimum amount of PBQ for determination of the product when mixed with casein was 100mg and a reaction time of 30 min. The product was stable for a period of 24h after the reacting by heating. The interaction of PBQ with casein was investigated by FT-IR, reflectance, Raman and EPR spectroscopies. The reaction between PBQ and casein in the solid state yields a radical species (p-benzosemiquinone) that it is stabilized by the presence of p-biphenolate and p-benzoquinone species in the solid state.
authors.affiliation: Departamento de Química, Universidade Estadual de Londrina, Cx P 6001, 86051-990 Londrina, PR, Brazil.
authors.forename: Isadora S
authors.initials: IS
authors.lastname: Vieira
authors.forename: Dimas A M
authors.initials: DA
authors.lastname: Zaia
authors.forename: Solange P
authors.initials: SP
authors.lastname: Balena
authors.forename: Antonio S
authors.initials: AS
authors.lastname: Mangrich
authors.forename: Henrique
authors.initials: H
authors.lastname: de Santana
mesh_terms: D016227:Benzoquinones / Q000737:chemistry* / Q000378:metabolism*; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D016227:Benzoquinones; D002364:Caseins; C004532:quinone