title: The copepod Tigriopus japonicus genomic DNA information (574Mb) and molecular anatomy.
abstract: The intertidal copepod, Tigriopus japonicus, has been recognized as a promising model species for marine environmental genomics. To obtain extensive genomic DNA sequences from this species, we sequenced genomic DNA from adult copepods using genomic sequencers GS-FLX and GS-FLX-Titanium and attained 1,914,995 reads (average read length 299.8 bp) including 574.2 Mb of genomic DNA information. After subjecting them to assembly, we acquired 193,642 contigs (total contigs length 129.7 Mb), and finally were able to obtain 10,894 unigenes (E-value>0.1; length>200 bp) containing 33,081,455 bp after a nonredundant (NR) blast search. In this paper, we summarize the genomic DNA sequences of T. japonicus and discuss its potential use in environmental genomics and ecotoxicological studies for uncovering mechanisms of environmental stresses and chemical toxicities to marine crustaceans.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea. jslee2@hanyang.ac.kr
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Ryeo-Ok
authors.initials: RO
authors.lastname: Kim
authors.forename: Dae-Sik
authors.initials: DS
authors.lastname: Hwang
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Han
authors.forename: Beom-Soon
authors.initials: BS
authors.lastname: Choi
authors.forename: Gyung Soo
authors.initials: GS
authors.lastname: Park
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Heum Gi
authors.initials: HG
authors.lastname: Park
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
mesh_terms: D000818:Animals; D033342:Copepoda / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D054750:Ecotoxicology; D004784:Environmental Monitoring; D005796:Genes; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA