title: Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma.
abstract: A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm x 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ultraviolet detection at 236 nm. Acetonitrile was used to simultaneously deproteinize rat plasma and extract metformin. The assay was linear in the concentration range of 0.33 mug-16.6 mug/ml with co-efficient of correlation 0.994. The retention time was 4.7 min. The method was found to be precise (% CV < 15%), accurate and suitable for pharmacokinetic study of orally administered metformin in rats.
authors.affiliation: Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440 033, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Wanjari
authors.forename: A W
authors.initials: AW
authors.lastname: There
authors.forename: M R
authors.initials: MR
authors.lastname: Tajne
authors.forename: C T
authors.initials: CT
authors.lastname: Chopde
authors.forename: S N
authors.initials: SN
authors.lastname: Umathe