Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical-cord-blood-derived CD34 cells.
Previous work has shown that optimal ex vivo expansion and differentiation of CD34(+) progenitor cells into neutrophils is by addition of Flt3-L, SCF and G-CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34(+) cells, however statistically significant changes in gene expression are not always consistent across UCB samples.
Gallagher
R C J
RC
Scottish National Blood Transfusion Service, Cell Therapy Group, MRC Centre for Regenerative Medicine, 49 Little France Crescent, Edinburgh, UK. ronnie.gallagher@ed.ac.uk
Tura-Ceide
O
O
Turner
M
M
Barclay
R
R
D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology*; D051157:Frizzled Receptors / Q000235:genetics*; D020869:Gene Expression Profiling; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D009504:Neutrophils / Q000166:cytology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000235:genetics*
D018952:Antigens, CD34; D018076:DNA, Complementary; D051157:Frizzled Receptors; D016298:Hematopoietic Cell Growth Factors; D012333:RNA, Messenger; D051153:Wnt Proteins