title: Study of enzymatic digestion of cellulose by small angle neutron scattering.
abstract: Small angle neutron scattering (SANS) was used to study the structure of Avicel (FD100) microcrystalline cellulose during enzymatic digestion. Digestions were performed in either of two modes: a static, quiescent mode or a dynamic mode using a stirred suspension recycled through a flow cell. The scattering pattern for as-received Avicel in D(2)O buffer is comprised of a low Q power law region resulting from the surface fractal character of the microcrystalline fibers and a high Q roll-off due to scattering from water-filled nanopores with radii approximately 20 A. For digestions in the dynamic mode the high Q roll-off decreased in magnitude within approximately 1 h after addition of enzymes, whereas in the static digestions no change was observed in the high Q roll-off, even after 60 h. These results indicate that only with significant agitation does enzyme digestion affect the structure of the nanopores.
authors.affiliation: Sandia National Laboratories, Albuquerque, New Mexico 87123, USA. mskent@sandia.gov
authors.forename: M S
authors.initials: MS
authors.lastname: Kent
authors.forename: G
authors.initials: G
authors.lastname: Cheng
authors.forename: J K
authors.initials: JK
authors.lastname: Murton
authors.forename: E L
authors.initials: EL
authors.lastname: Carles
authors.forename: D C
authors.initials: DC
authors.lastname: Dibble
authors.forename: F
authors.initials: F
authors.lastname: Zendejas
authors.forename: M A
authors.initials: MA
authors.lastname: Rodriquez
authors.forename: H
authors.initials: H
authors.lastname: Tran
authors.forename: B
authors.initials: B
authors.lastname: Holmes
authors.forename: B A
authors.initials: BA
authors.lastname: Simmons
authors.forename: B
authors.initials: B
authors.lastname: Knierim
authors.forename: M
authors.initials: M
authors.lastname: Auer
authors.forename: J L
authors.initials: JL
authors.lastname: Banuelos
authors.forename: J
authors.initials: J
authors.lastname: Urquidi
authors.forename: R P
authors.initials: RP
authors.lastname: Hjelm
mesh_terms: D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D033363:Neutron Diffraction / Q000379:methods*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D002482:Cellulose