title: Volatile profiles of human skin cell cultures in different degrees of senescence.
abstract: It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells.
authors.affiliation: Universidad Técnica Federico Santa María, Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile. cristian.acevedo@usm.cl
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Acevedo
authors.forename: Elizabeth Y
authors.initials: EY
authors.lastname: Sanchez
authors.forename: Juan G
authors.initials: JG
authors.lastname: Reyes
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Young
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence; D002648:Child; D002675:Child, Preschool; D019295:Computational Biology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D016018:Least-Squares Analysis; D053858:Metabolic Networks and Pathways; D025341:Principal Component Analysis; D012867:Skin / Q000166:cytology* / Q000378:metabolism*; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis*; D014835:Volatilization
chemical_list: D055549:Volatile Organic Compounds