Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology.
SOX2 is a gene located on chromosome 3q26.33 that encodes a transcription factor important to maintenance of embryonic neural crest stem cell pluripotency. We have identified rare SOX2-immunoreactive cells in normal human skin at or near the established stem cell niches. Three subsets of SOX2-positive cells were defined in these regions: those expressing only SOX2 and those that co-expressed SOX2 and either CK20 or microphthalmia-associated transcription factor, which are consistent with dichotomous differentiation of SOX2-expressing precursors along neuroendocrine (Merkel cell) or melanocytic lines, respectively. Examination of Merkel cell carcinomas confirmed nuclear SOX2 expression in this tumor type. In human patient melanoma, strong nuclear expression of SOX2 was noted in a subset of tumors, and the ability to detect SOX2 in lesional cells significantly correlated with primary tumor thickness in a survey cohort. To assess the potential role of SOX2 in melanoma growth, an in vivo tumorigenesis assay was used. Whereas SOX2 knockdown failed to influence proliferation of cultured melanoma cells in vitro, tumor xenografts generated with the SOX2-knockdown cell line showed significant decrease in mean tumor volume as compared with controls. In aggregate, these findings suggest that SOX2 is a novel biomarker for subpopulations of normal skin cells that reside in established stem cell niches and that might relate to Merkel cell and melanocyte ontogeny and tumorigenesis.
Laga
Alvaro C
AC
Department of Pathology, Program in Dermatopathology, Boston, MA 02115, USA. alaga@rics.bwh.harvard.edu
Lai
Chiou-Yan
CY
Zhan
Qian
Q
Huang
Susan J
SJ
Velazquez
Elsa F
EF
Yang
Qinghong
Q
Hsu
Mei-Yu
MY
Murphy
George F
GF
D000818:Animals; D015266:Carcinoma, Merkel Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008544:Melanocytes / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018862:Merkel Cells / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D051379:Mice; D016513:Mice, SCID; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D055153:Stem Cell Niche / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors