Detection of Notch signaling molecules in cemento-ossifying fibroma of the jaws.
BACKGROUND
The aim of this study was to evaluate the roles of Notch signaling in the oncogenesis and cytodifferentiation of cemento-ossifying fibroma, the expressions of Notch receptors and ligands were detected in COF and normal jaw bones.


METHODS
The expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively in 16 cases of normal bone tissues and 12 cases of COF of the jaws.


RESULTS
The mRNAs expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected in all specimens. The expression levels of mRNAs in COF were higher than those in normal bones. In COF, Notch proteins staining were showed extensively distribution in fibroblasts and osteoblasts. In normal bone tissue, Notch proteins were expressed in osteoblasts, whereas proteins staining were weaker than those in COF, but no detection in fibroblast-like bone marrow stroma cells. The expressions of Notch receptors and ligands were not detected in cementum-like products or bone matrices.


CONCLUSIONS
Our data suggest that Notch signaling may participate in controlling cell differentiation and proliferation in normal bone and COF of the jaws. Notch signaling disorder may be a molecular incident in COF occurrence and development.
Zhang
Tong-Han
TH
Department of Oral and Maxillofacial Surgery, Sun Yat-Sen University, Guangzhou, China.
Liu
Hai-Chao
HC
Liao
Gui-Qing
GQ
Liang
Yu-Jie
YJ
Chu
Mei
M
Wan
Chang-Qing
CQ
Liang
Li-Zhong
LZ
Zheng
Guang-Sen
GS
D001854:Bone Marrow Cells / Q000473:pathology; D001857:Bone Matrix / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D007568:Jaw / Q000473:pathology; D007573:Jaw Neoplasms / Q000473:pathology*; D008565:Membrane Proteins / Q000032:analysis*; D009808:Odontogenic Tumors / Q000473:pathology*; D010006:Osteoblasts / Q000473:pathology; D051881:Receptor, Notch1 / Q000032:analysis*; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000032:analysis*; D000072099:Serrate-Jagged Proteins; D017154:Stromal Cells / Q000473:pathology
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C490504:JAG2 protein, human; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; C499374:NOTCH3 protein, human; D051881:Receptor, Notch1; D000071656:Receptor, Notch3; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins