Role of C-terminal region of yeast ADP/ATP carrier 2 protein: dynamics of flexible C-terminal arm.
BACKGROUND
The ADP/ATP carrier catalyzes the exchange of ADP and ATP across the inner mitochondrial membrane.


METHODS
The molecular dynamics of modeled yeast type 2 AAC (yAAC2) was analyzed and molecular parameters were determined.


RESULTS
The yAAC2 C-terminal moved flexibly and a negative electrostatic potential field (ESP) was located in the C-terminal region. The ESP field is always located in the C-terminal area during C-terminal truncation (d1-d9). Further C-terminal truncation occurred on field invagination into the core region (d11, d14, d16). The 2-6 C-terminal amino acid truncation did not affect the biological activity. The d7-d9 truncated mutants lost their biological function.


CONCLUSIONS
A critical point in yAAC2 function was shown between d6 and d7 C-terminal truncation. The C-terminal structure of yAAC2 is thought to be involved in biological function control.
Ohkura
Kazuto
K
Chiba Institute of Science, Choshi, Chiba, 288-0025, Japan. kohkura@sag.bekkoame.ne.jp
Hori
Hitoshi
H
Shinohara
Yasuo
Y
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000502:physiology*; D008956:Models, Chemical; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000502:physiology*; D055672:Static Electricity; D013329:Structure-Activity Relationship
C112225:PET9 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000226:Mitochondrial ADP, ATP Translocases