Influence of the dark/light rhythm on the effects of UV radiation in the eyestalk of the crab Neohelice granulata.
Crustaceans are interesting models to study the effects of ultraviolet (UV) radiation, and many species may be used as biomarkers for aquatic contamination of UV radiation reaching the surface of the Earth. Here, we investigated cell damage in the visual system of crabs Neohelice granulata that were acclimated to either 12L:12D, constant light, or constant dark, and were exposed to UVA or UVB at 12:00h (noon). The production of reactive oxygen species (ROS), antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (LPO) damage, catalase activity, and pigment dispersion in the eye were evaluated. No significant differences from the three groups of controls (animals acclimated to 12L:12D, or in constant light, or not exposed to UV radiation) were observed in animals acclimated to 12L:12D, however, crabs acclimated to constant light and exposed to UV radiation for 30min showed a significant increase in ROS concentration, catalase activity, and LPO damage, but a decrease in ACAP compared with the controls. Crabs acclimated to constant darkness and exposed to UV for 30min showed a significantly increased ROS concentration and LPO damage, but the ACAP and catalase activity did not differ from the controls (animals kept in the dark while the experimental group was being exposed to UV radiation). Pigment dispersion in the pigment cells of eyes of animals acclimated to constant light was also observed. The results indicate that UVA and UVB alter specific oxidative parameters; however, the cell damage is more evident in animals deviated from the normal dark/light rhythm.
Vargas
Marcelo Alves
MA
Programa de Pós-Graduação em Ciências Morfológicas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro-UFRJ, 21941-590, Rio de Janeiro, RJ, Brazil.
Geish
Marcio Alberto
MA
Maciel
Fabio Everton
FE
Cruz
Bruno Pinto
BP
Filgueira
Daza de Moraes Vaz Batista
Dde M
Ferreira
Gabrielle de Jesus
Gde J
Nery
Luiz Eduardo Maia
LE
Allodi
Silvana
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000528:radiation effects; D003386:Brachyura / Q000502:physiology / Q000528:radiation effects*; D002374:Catalase / Q000378:metabolism / Q000528:radiation effects*; D002940:Circadian Rhythm; D004249:DNA Damage; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008297:Male; D017440:Photoperiod; D010860:Pigments, Biological / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D000975:Antioxidants; D010860:Pigments, Biological; D017382:Reactive Oxygen Species; D002374:Catalase