Radiosynthesis and micro-SPECT imaging of 99mTc-dendrimer poly(amido)-amine folic acid conjugate.
Acetylated (Ac) dendrimer poly(amido)-amine (PAMAM) generation 5 (G5) reacted with folic acid (FA), followed by reacting with 2-(p-isothiocyanatobenzyl)-6-methyl-diethylenetria minepentaacetic acid (1B4M DTPA) to form the conjugate of Ac-G5-FA-1B4M DTPA which was further radiolabeled with (99m)Tc. The radiochemical yield is up to 98.9% with excellent in vitro/in vivo stability, rapid blood clearance and certain tumor accumulation which was further confirmed by micro-SPECT imaging study.
Zhang
Yuanqing
Y
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 BaoJia Road, Shanghai 201800, PR China.
Sun
Yanhong
Y
Xu
Xiaoping
X
Zhu
Hua
H
Huang
Liliang
L
Zhang
Xuezhu
X
Qi
Yujin
Y
Shen
Yu-Mei
YM
D000818:Animals; D050091:Dendrimers / Q000138:chemical synthesis*; D005492:Folic Acid / Q000138:chemical synthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging; D011073:Polyamines / Q000138:chemical synthesis*; D011868:Radioisotopes / Q000737:chemistry; D013667:Technetium / Q000737:chemistry*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D011868:Radioisotopes; D013667:Technetium; D005492:Folic Acid