Specific nucleic acid detection using photophysical properties of quantum dot probes.
In the present study, we report a novel separation-free method to detect and quantify avian influenza virus A (H5N1) nucleic acid without amplification, based on the alteration of photophysical parameters of quantum dot (QD) probes after hybridization with specific complementary target DNA. The target DNA was quantified in a custom-made portable device by simultaneously measuring lifetime and quenching of the QD probes. QD probes (25-mer) showed a 30% lifetime reduction and 40% fluorescence quenching when hybridized with complementary 25-mer target DNA. In comparison with a conventional QD-based assay, this assay provides a simple quantitation of nucleic acids with a single labeling step.
Lim
Sun Hee
SH
Diagnostics, Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam City, Gyeonggi-do, Korea, 443-270. sunhlim@ip-korea.org
Buchy
Philippe
P
Mardy
Sek
S
Kang
Moon Sik
MS
Yu
Alexey Dan Chin
AD
D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D007700:Kinetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D045663:Quantum Dots
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D004247:DNA