[Analysis of the relationship between Glut-4 gene polymorphism and blood-fat traits in induced hyperglycemia mice].
This study is to investigate the effect of diet and gene on blood-fat trait of the individual mouse. One hundred and twenty mice were fed with high fat emulsion for 4 weeks. Then the genotypes of Glut-4 were analyzed by PCR-SSCP to investigate the effect on blood-fat traits and some organ performance of mice. After fed with high fat emulsion for 4 weeks, 98 mice suffered from hyperlipidemia, while 22 mice did not. And a T/A mutation was found in 188 targeted fragments we amplified at 8,521 site, and association analysis showed that the mice carried BB genotype had higher TC, TG and LW/BW level than those of the mice with AA genotype (P < 0.05). Different Glut-4 genotypes show different sensitivities to high fat emulsion on mice.
Chen
Qin
Q
College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Wang
Wen-Jun
WJ
Shang-Guan
Xin-Chen
XC
Xu
Ming-Sheng
MS
D000818:Animals; D051275:Glucose Transporter Type 4 / Q000235:genetics*; D006943:Hyperglycemia / Q000097:blood* / Q000235:genetics*; D008055:Lipids / Q000097:blood; D051379:Mice; D008820:Mice, Obese / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
D051275:Glucose Transporter Type 4; D008055:Lipids; C495693:Slc2a4 protein, mouse