Ricin activity assay by direct analysis in real time mass spectrometry detection of adenine release.
Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, multistep analysis, or a combination thereof. We report a single-step, real-time ricin activity assay that requires little or no sample preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by ricin was determined to be 53 +/- 2 pmol adenine per picomole of ricin per hour. This procedure can be readily adapted to any enzyme for which a reactant or product of low molecular weight (up to approximately 600) can be identified.
Bevilacqua
Vicky L H
VL
U.S. Army Edgewood Chemical Biological Center, 5183 Black Hawk Road, Aberdeen Proving Ground, Maryland 21010-5424, USA. Vicky.bevilacqua@us.army.mil
Nilles
J Michael
JM
Rice
Jeffrey S
JS
Connell
Theresa R
TR
Schenning
Amanda M
AM
Reilly
Lisa M
LM
Durst
H Dupont
HD
D000225:Adenine / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012276:Ricin / Q000378:metabolism*
D004247:DNA; D012276:Ricin; D000225:Adenine