title: Effect of taxifolin glycoside on atopic dermatitis-like skin lesions in NC/Nga mice.
abstract: Increased levels of eosinphils, IgE, IL-4, 5, and 13 and pro-inflammatory factors (COX-2, iNOS) are observed in patients with atopic dermatitis (AD). Taxifolin 3-O-beta-D-glucopyranoside (TAX) from the roots of Rhododendron mucronulatum (RM) was examined to determine whether its immunomodulatory effect was applicable for treating atopic dermatitis.A total of 7 groups of NC/Nga mice with AD were treated by topical application or intraperitoneal injection of TAX for 4 weeks. Follow-up evaluations were done to assess the changes in clinical observations, eosinophil counts, and levels of IgE, cytokines, COX-2 and iNOS.In the clinical observation during the experimental period, TAX treatment significantly reduced the severity of AD-like lesions induced in NC/Nga mice. Eosinophil and IgE levels decreased after treatment of the animals with TAX. TAX may thus be associated with improvement of eosinophil-related allergic diseases. The expression of cytokines (IL-4, 5 and 13) was significantly inhibited in the TAX-treated group, suggesting that TAX might play an immunoregulatory role associated with AD. In RT-PCR, iNOS and COX-2 expression levels were reduced in the TAX-treated group. In western blotting, the expression levels of iNOS and COX-2 were also reduced in the TAX-treated group.These findings suggest that TAX is effective for the treatment of AD by preventing the production of inflammatory cytokines and by reducing skin inflammation.
authors.affiliation: College of Medicine, Chung-Ang University, Seoul 156-756, South Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Sun Eun
authors.initials: SE
authors.lastname: Choi
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Jeong
authors.forename: Kwan Hee
authors.initials: KH
authors.lastname: Park
authors.forename: Nam Ju
authors.initials: NJ
authors.lastname: Moon
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Joo
authors.forename: Chung Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Choi
authors.forename: Kapsok
authors.initials: K
authors.lastname: Li
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Lee
authors.forename: Min Won
authors.initials: MW
authors.lastname: Lee
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Seo
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000032:analysis; D003521:Cyclopropanes; D016207:Cytokines / Q000097:blood; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D029793:Rhododendron / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003521:Cyclopropanes; D016207:Cytokines; D006027:Glycosides; D010936:Plant Extracts; D007073:Immunoglobulin E; D011794:Quercetin; C003377:taxifolin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; C029402:diphenylcyclopropenone