title: Role of C-terminal region of yeast ADP/ATP carrier 2 protein: dynamics of flexible C-terminal arm.
abstract: BACKGROUND
  The ADP/ATP carrier catalyzes the exchange of ADP and ATP across the inner mitochondrial membrane.
  
  
  METHODS
  The molecular dynamics of modeled yeast type 2 AAC (yAAC2) was analyzed and molecular parameters were determined.
  
  
  RESULTS
  The yAAC2 C-terminal moved flexibly and a negative electrostatic potential field (ESP) was located in the C-terminal region. The ESP field is always located in the C-terminal area during C-terminal truncation (d1-d9). Further C-terminal truncation occurred on field invagination into the core region (d11, d14, d16). The 2-6 C-terminal amino acid truncation did not affect the biological activity. The d7-d9 truncated mutants lost their biological function.
  
  
  CONCLUSIONS
  A critical point in yAAC2 function was shown between d6 and d7 C-terminal truncation. The C-terminal structure of yAAC2 is thought to be involved in biological function control.
authors.affiliation: Chiba Institute of Science, Choshi, Chiba, 288-0025, Japan. kohkura@sag.bekkoame.ne.jp
authors.forename: Kazuto
authors.initials: K
authors.lastname: Ohkura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hori
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000502:physiology*; D008956:Models, Chemical; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000502:physiology*; D055672:Static Electricity; D013329:Structure-Activity Relationship
chemical_list: C112225:PET9 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000226:Mitochondrial ADP, ATP Translocases