title: Ultrasensitive, multiplexed detection of cancer biomarkers directly in serum by using a quantum dot-based microfluidic protein chip.
abstract: Sensitive and selective detection for cancer biomarkers are critical in cancer clinical diagnostics. Here we developed a microfluidic protein chip for an ultrasensitive and multiplexed assay of cancer biomarkers. Aqueous-phase-synthesized CdTe/CdS quantum dots (aqQDs) were employed as fluorescent signal amplifiers to improve the detection sensitivity. Secondary antibodies (goat anti-mouse IgG) were conjugated to luminescent CdTe/CdS QDs to realize a versatile fluorescent probe that could be used for multiplexed detection in both sandwich and reverse phase immunoassays. We found that our microfluidic protein chip not only possessed ultrahigh femtomolar sensitivity for cancer biomarkers, but was selective enough to be directly used in serum. This protein chip thus combines the high-throughput capabilities of a microfluidic network with the high sensitivity and multicolor imaging ability offered by highly fluorescent QDs, which can become a promising diagnostic tool in clinical applications.
authors.affiliation: Laboratory of Advanced Materials, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Hu
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Haoting
authors.initials: H
authors.lastname: Lu
authors.forename: Lixing
authors.initials: L
authors.lastname: Weng
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D002272:Carcinoembryonic Antigen / Q000097:blood / Q000276:immunology; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D046210:Microfluidic Analytical Techniques; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D045663:Quantum Dots; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; D000509:alpha-Fetoproteins