title: [Effects of water stress on protein expression and physiological properties of different genotype wheat (Triticum aestivum L.) sprouts].
abstract: With drought-resistant wheat (Triticum aestivum) cultivar Changwu 134 and drought-sensitive cultivar Zhengyin 1 as test materials, and by using -1.2 MPa PEG 6000 to treat their seeds, this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions. SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39.5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts. Under normal water supply, the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth. This protein was preliminarily named as water-sensitive protein, due to its water-sensitivity and of newly discovered protein. The determinations of physiological properties showed that under water stress, the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content, but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China. daiming5014@163.com
authors.forename: Ming
authors.initials: M
authors.lastname: Dai
authors.forename: Xi-ping
authors.initials: XP
authors.lastname: Deng
authors.forename: Shu-shen
authors.initials: SS
authors.lastname: Yang
authors.forename: Rang
authors.initials: R
authors.lastname: Cao
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Guo
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
mesh_terms: D005838:Genotype; D010940:Plant Proteins / Q000378:metabolism*; D036226:Seedlings / Q000737:chemistry / Q000502:physiology; D013312:Stress, Physiological; D014908:Triticum / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014867:Water / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014867:Water