2-Butoxyethanol model of haemolysis and disseminated thrombosis in female rats: a preliminary study of the vascular mechanism of osteoarthritis in the temporomandibular joint.
Female rats develop haemolytic anaemia and disseminated thrombosis and infarction in multiple organs, including bone, when exposed to 2-butoxyethanol (BE). There is growing evidence that vascular occlusion of the subchondral bone may play a part in some cases of osteoarthritis. The subchondral bone is the main weight bearer as well as the source of the blood supply to the mandibular articular cartilage. Vascular occlusion is thought to be linked to sclerosis of the subchondral bone associated with disintegration of the articular cartilage. The aim of this study was to find out whether this model of haemolysis and disseminated thrombosis supports the vascular hypothesis of osteoarthritis. Six female rats were given BE orally for 4 consecutive days and the two control rats were given tap water alone. The rats were killed 26 days after the final dose. The mandibular condyles showed histological and radiological features consistent with osteoarthritis in three of the four experimental rats and in neither of the control rats. These results may support the need to explore the vascular mechanism of osteoarthritis further.
Amir
G
G
Department of Pathology, Hadassah Medical Center, The Hebrew University Hadassah School of Medicine, Kiryat Hadassah, Jerusalem il-91120, Israel. gails@cc.huji.ac.il
Goldfarb
A W
AW
Nyska
M
M
Redlich
M
M
Nyska
A
A
Nitzan
D W
DW
D000743:Anemia, Hemolytic / Q000150:complications*; D000818:Animals; D001842:Bone and Bones / Q000098:blood supply*; D002358:Cartilage, Articular / Q000098:blood supply / Q000000981:diagnostic imaging; D019902:Chondrocytes / Q000473:pathology; D004195:Disease Models, Animal; D004211:Disseminated Intravascular Coagulation / Q000150:complications*; D004987:Ethers / Q000009:adverse effects*; D005026:Ethylene Glycols / Q000009:adverse effects*; D005260:Female; D006132:Growth Plate / Q000473:pathology; D007238:Infarction / Q000150:complications*; D008335:Mandibular Condyle / Q000098:blood supply / Q000000981:diagnostic imaging; D010003:Osteoarthritis / Q000209:etiology*; D054850:Osteophyte / Q000473:pathology; D010026:Osteosclerosis / Q000209:etiology; D011859:Radiography; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012997:Solvents / Q000009:adverse effects*; D013705:Temporomandibular Joint Disorders / Q000209:etiology*; D051598:Whole Body Imaging
D004987:Ethers; D005026:Ethylene Glycols; D012997:Solvents; C017096:n-butoxyethanol