Mesenchymal stem cells and bovine bone mineral in sinus lift procedures--an experimental study in sheep.
BACKGROUND
New reconstructive and less invasive methods have been searched to optimize bone formation and osseointegration of dental implants in maxillary sinus augmentation.


OBJECTIVE
The aim of the presented ovine split-mouth study was to compare bovine bone mineral (BBM) alone and in combination with mesenchymal stem cells (MSCs) regarding their potential in sinus augmentation.


METHODS
Bilateral sinus floor augmentations were performed in six adult sheep. BBM and MSCs were placed into the test side and only BBM in the contra-lateral control side of each sheep. Animals were sacrificed after 8 and 16 weeks. Augmentation sites were analyzed by computed tomography, histology, and histomorphometry.


RESULTS
The initial volumes of both sides were similar and did not change significantly with time. A tight connection between the particles of BBM and the new bone was observed histologically. Bone formation was significantly (p = 0.027) faster by 49% in the test sides.


CONCLUSIONS
The combination of BBM and MSCs accelerated new bone formation in this model of maxillary sinus augmentation. This could allow early placement of implants.
Sauerbier
Sebastian
S
Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, Freiburg, Germany. sebastian.sauerbier@uniklinik-freiburg.de
Stubbe
Kerstin
K
Maglione
Michele
M
Haberstroh
Jörg
J
Kuschnierz
Jens
J
Oshima
Toshiyuki
T
Xavier
Samuel Porfirio
SP
Brunnberg
Leo
L
Schmelzeisen
Rainer
R
Gutwald
Ralf
R
D000818:Animals; D001842:Bone and Bones; D002417:Cattle; D002448:Cell Adhesion; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008903:Minerals; D010256:Paranasal Sinuses / Q000601:surgery*; D012756:Sheep
D008903:Minerals