[Characterization of Bacillus thuringiensis spoIII D gene mutation].
OBJECTIVE
Construction and characterization of a spoIII D gene deletion mutant of Bacillus thuringiensis.


METHODS
Scanning electron microscopy and spore formation analysis were used to detect the ability of sporulation and formation of crystal protein in both the mutant and the wild strain. SDS-PAGE analysis was used to detect the expression of crystal protein.


RESULTS
Scanning electron microscopy and spore formation analysis showed that spores were hardly produced and the crystal existed in the spoIII D deletion strain. SDS-PAGE results showed that the expression of cry gene in the mutant was decreased in Luria-Bertani medium, but not affected obviously in Schaeffer's sporulation medium (SSM).


CONCLUSIONS
This indicated that the spoIII D gene was one of the essential genes for the sporulation of Bacillus thuringiensis, and influenced the expression of crystal protein.
Zhang
Qianqian
Q
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. peregrine2018@gmail.com
Shu
Changlong
C
Zhang
Jie
J
Huang
Dafang
D
Song
Fuping
F
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C071977:SpoIIID protein, Bacillus; D014157:Transcription Factors