Mercuric mercaptide of penicillenic acid, a novel hapten for relevant immunoassay, synthesized from penicillin.
The synthesis of mercuric mercaptide of penicillenic acid (MMPA) has been the basis for detection of penicillin for nearly 40 years (J. Pharm. Pharmacol., 1972, 24, 790; Chinese Pharmacopoeia Ed. II, 1995). Herein, experiments were performed on: (1) synthesis of MMPA used as a novel mercuric hapten, (2) preparation of mercuric antigen of MMPA-BSA, (3) production of antibodies by rabbits immunized with the antigen, and (4) properties of the antibodies studied by ELISA. The results show that: (1) the antigen is safe for immunized animals; (2) high titer antibodies against MMPA are obtained implying good immunogenicity of the antigen; (3) antisera show slightly higher affinity to OVA-GHS-HgCl than OVA-GSH, indicating weak specific affinity of antisera against mercuric ion. Even the weak specific affinity, the hapten and its antigen have potential uses in immunoassays of mercuric ion in environment and food samples, because of easy chemical selective conversion from mercuric ion to MMPA and complete decomposition of un-reacted penicillin in acidic solution.
Xie
Peng
P
Laboratory of Analytical Biochemistry & Bio-separation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China.
Tao
Xi
X
Xu
Wu
W
Fan
Liu-Yin
LY
Zhang
Wei
W
Zhi
Yue-E
YE
Zhou
Pei
P
Cao
Cheng-Xi
CX
D000818:Animals; D000906:Antibodies / Q000097:blood; D000918:Antibody Specificity; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009941:Organomercury Compounds / Q000138:chemical synthesis / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010406:Penicillins / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D006241:Haptens; D009941:Organomercury Compounds; D010406:Penicillins; C547708:mercuric mercaptide of penicillenic acid; D012710:Serum Albumin, Bovine; D010047:Ovalbumin