Ophiostoma tsotsi sp. nov., a wound-infesting fungus of hardwood trees in Africa.
Polymorphic sequence-characterised marker assays from a recent diversity study on the Ascomycete fungus Ophiostoma quercus reported that some isolates from Africa were genetically distinct from O. quercus. In the present study, these African isolates were compared with authentic O. quercus isolates by evaluating morphological characters, growth in culture, mating compatibility and DNA sequence data. The isolates from Africa were morphologically similar to O. quercus, presenting Pesotum and Sporothrix synanamorphs in culture. Phylogenetic analyses of the ribosomal internal transcribed spacer regions 1 and 2, beta-tubulin and translation elongation factor 1-alpha gene regions confirmed that the African group represents a distinct species within the hardwood lineage of the O. piceae complex, closely related to O. ulmi and O. himal-ulmi. Mating studies between O. quercus and the African isolates showed that isolates mated predominantly with those of their own group, although there were rare cases of fertile crosses between the groups. Isolates residing in the African lineage are described here as a new species, O. tsotsi sp. nov.
Grobbelaar
Joha W
JW
Department of Genetics, University of Pretoria, Pretoria 0002, South Africa.
de Beer
Z Wilhelm
ZW
Bloomer
Paulette
P
Wingfield
Michael J
MJ
Wingfield
Brenda D
BD
D000349:Africa; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D055156:Ophiostoma / Q000145:classification* / Q000166:cytology / Q000502:physiology; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000235:genetics; D014197:Trees / Q000382:microbiology*; D014404:Tubulin / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020648:Peptide Elongation Factor 1; D000091262:TEA Domain Transcription Factors; C057182:TEAD1 protein, human; D014157:Transcription Factors; D014404:Tubulin