Two unhealthy dietary habits featuring a high fat content and a sucrose-containing beverage intake, alone or in combination, on inducing metabolic syndrome in Wistar rats and C57BL/6J mice.
To establish animal models with diet-induced metabolic disorders similar to human metabolic syndrome, 2 unhealthy dietary habits featuring a high fat content and a sucrose-containing beverage intake, alone or in combination, were tested on Wistar rats and C57BL/6J mice. The 2 dietary habits were, respectively, simulated by feeding a high-fat diet (regimen A) or additionally providing 30% sucrose (wt/vol) in the drinking water (regimen B). Using a 2 x 2 factorial design, 4 groups of animals were fed chow diet plus plain water (group C), high-fat diet (30% [wt/wt] fat) plus plain water (group A), chow diet plus sucrose in drinking water (group B), and high-fat diet plus sucrose in drinking water (group AB) for 26 weeks. In Wistar rats, regimen B caused a significant increase in visceral fat; serum levels of lipids, glucose, insulin, and uric acid; insulin resistance; and blood pressure, whereas regimen A only caused a significant increase in visceral fat and serum insulin levels (P < .05). In contrast, regimen A induced a full array of metabolic syndrome in C57BL/6J mice; but regimen B only caused slight obesity and hyperlipidemia. In both Wistar rats and C57BL/6J mice, there were no additive effects of the 2 regimens, indicated by significant interactions between regimens A and B on the metabolic indexes measured. These results show that, in terms of inducing metabolic syndrome, Wistar rats are more responsive to sucrose water regimen, whereas C57BL/6J mice are more responsive to the high-fat diet regimen.
Chen
Gou-Chun
GC
Institute of Nutrition, China Medical University, Taichung 404, Taiwan.
Huang
Chun-Yin
CY
Chang
Mei-Yu
MY
Chen
Chi-Hua
CH
Chen
Shiow-Wen
SW
Huang
Ching-Jang
CJ
Chao
Pei-Min
PM
D000818:Animals; D001628:Beverages / Q000009:adverse effects*; D001786:Blood Glucose / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D019422:Dietary Sucrose / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D006949:Hyperlipidemias / Q000209:etiology; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000209:etiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D004041:Dietary Fats; D019422:Dietary Sucrose; D007328:Insulin; D008055:Lipids