Immunological reactivity of blood from healthy humans to the rAls3p-N vaccine protein.
We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.
Baquir
Beverlie
B
Divisions of Infectious Diseases, Los Angeles Biomedical Research Institute, Harbor-University of California at Los Angeles (UCLA) Medical Center, USA.
Lin
Lin
L
Ibrahim
Ashraf S
AS
Fu
Yue
Y
Avanesian
Valentina
V
Tu
Ang
A
Edwards
John
J
Spellberg
Brad
B
D000908:Antibodies, Fungal / Q000097:blood*; D002175:Candida / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000276:immunology*; D005657:Fungal Vaccines / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood
D000908:Antibodies, Fungal; D016207:Cytokines; D005656:Fungal Proteins; D005657:Fungal Vaccines; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E