Profiling gene expression of whole cytochrome P450 superfamily in human bronchial and peripheral lung tissues: Differential expression in non-small cell lung cancers.
Susceptibility to lung diseases, such as lung cancer and chronic obstructive pulmonary disease, is largely influenced by the metabolic capacity of lung tissues. This capacity is partly determined by the expression profile of the cytochromes P450 (CYPs), a superfamily of enzymes that have relevant catalytic properties toward exogenous and endogenous compounds. Using quantitative real-time RT-PCR, we conducted a comprehensive analysis of the expression profile of the 57 human CYP genes in non-tumoral (bronchial mucosa and pulmonary parenchyma) and tumoral lung tissues of 18 patients with non-small cell lung cancer. This study highlights (i) inter-individual variations in lung expression for some CYPs, (ii) different CYP expression patterns between bronchial mucosa and pulmonary parenchyma, that indicate distinctive susceptibility of these tissues toward the deleterious effects of inhaled chemical toxicants and carcinogens, (iii) high intertumoral variability, that could have major implications on lung tumor response to anti-cancer drugs.
Leclerc
Julie
J
Equipe d'accueil EA2679, Faculté de Médecine Pôle Recherche, Université Lille Nord de France, Place de Verdun, Lille, France. julie.leclerc-2@univ-lille2.fr
Tournel
Gilles
G
Courcot-Ngoubo Ngangue
Elisabeth
E
Pottier
Nicolas
N
Lafitte
Jean-Jacques
JJ
Jaillard
Sophie
S
Mensier
Eric
E
Lhermitte
Michel
M
Broly
Franck
F
Lo-Guidice
Jean-Marc
JM
D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000033:anatomy & histology / Q000201:enzymology* / Q000473:pathology / Q000502:physiology; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System