title: The effects of Nigella sativa on bile duct ligation induced-liver injury in rats.
abstract: Nigella sativa (NS) has been shown to have antioxidant and antiinflammatory activities in different conditions. The goal of this study was to evaluate the effects of NS on cholestatic liver injury in rats. Thirty rats were recruited in the study as follows: Group 1, Bile duct ligation (BDL) (n = 10); Group 2, BDL plus NS (n = 10); and Group 3, Sham (n = 10). Bile duct ligated group received 0.2 mL kg(-1) dose of NS intraperitoneally daily throughout 14 days. Liver damage and cholestasis were determined by the biochemical and the pathologic examination. Data showed a decrease in gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activities of the NS treated rats when compared with BDL group (p < 0.001 for GGT and p < 0.05 for others). The NS treated rats' tissue levels of total oxidant status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO) were significantly lower than that of the BDL group (p < 0.01 for all). Increases in total antioxidant capacity (TAC) and catalase (CAT) levels were statistically significant in the NS treated rats compared to BDL group (p < 0.01 for both). On the other hand, administration of NS in the rats with biliary obstruction resulted in inhibition of necro-inflammation. These results indicate that NS exerts a therapeutic effect on cholestatic liver injury in bile duct ligated rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.
authors.affiliation: Department of General Surgery, Gaziantep University School of Medicine, Turkey. sacid.coban@mountsinai.org
authors.forename: Sacid
authors.initials: S
authors.lastname: Coban
authors.forename: Fahrettin
authors.initials: F
authors.lastname: Yildiz
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Terzi
authors.forename: Behcet
authors.initials: B
authors.lastname: Al
authors.forename: Nurten
authors.initials: N
authors.lastname: Aksoy
authors.forename: Muharrem
authors.initials: M
authors.lastname: Bitiren
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001652:Bile Ducts / Q000601:surgery; D002374:Catalase / Q000378:metabolism; D002779:Cholestasis / Q000150:complications* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008026:Ligation; D008107:Liver Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D031881:Nigella sativa / Q000737:chemistry*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase