Expression of the Parkinson's disease-related protein DJ-1 during neural stem cell proliferation.
Neural stem cells (NSCs) arise through neurogenesis, and comprise all newly-formed neurons in the central and peripheral nervous systems. DJ-1 is associated with autosomal recessive familial Parkinson's disease (PD). The relationship between DJ-1 and NSC proliferation may shed light on the underlying pathogenesis of, and potential treatments for, PD. To investigate the relationship between DJ-1 and NSCs, embryonic cortical NSCs were isolated and cultured from E14 fetal rats. Immunocytochemistry, flow cytometry, and immunohistochemistry were applied to evaluate DJ-1 expression in proliferating NSCs. We found that DJ-1 was co-expressed with nestin, a marker of progenitors, during NSC proliferation from days 1-7. The present results suggest that DJ-1 is co-expressed with nestin in NSCs during proliferation.
Yan
Hui
H
State Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, China.
Pu
Xiao-Ping
XP
D000818:Animals; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D039902:Multipotent Stem Cells / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D000071617:Protein Deglycase DJ-1; D051381:Rats; D017207:Rats, Sprague-Dawley
D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1