Changes in cell proliferation in the subventricular zone of the brain in adult rats given beta-amyloid peptide (25-35).
The effects of intracerebroventricular administration of fragment (25-35) of beta-amyloid peptide [Abeta(25-35)] on cell proliferation in the subventricular zone of the dentate gyrus of the hippocampus in adult rats were analyzed. Animals received doses of 15 nmol of pre-aggregated Abeta(25-35) or the Abeta(35-25) control peptide, or solvent (sterile water) into the lateral ventricles. On post-injection days 1-5, rats received intraperitoneal injections of the thymidine analog 5-bromo-2'-deoxyuridine (BrdU). BrdU incorporated into DNA was detected immunohistochemically on frontal brain sections six and 12 days after peptide administration. At six days, the numbers of BrdU-containing cells in the subventricular zone showed no differences between the study groups. At 12 days, the total number of BrdU-positive cells decreased significantly in all study groups. At the same time, the number of labeled cells in rats given Abeta(25-35) was significantly greater in this brain zone than in animals given water or the control peptide. Thus, Abeta(25-35) significantly increased cell proliferation in the subventricular zone after intracerebroventricular administration.
Stepanichev
M Yu
MY
Laboratory for the Functional Biochemistry of the Nervous System, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia.
Moiseeva
Yu V
YV
Lazareva
N A
NA
Onufriev
M V
MV
Gulyaeva
N V
NV
D000375:Aging; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D055503:Protein Multimerization; D051381:Rats; D017208:Rats, Wistar; D055153:Stem Cell Niche / Q000502:physiology*; D013997:Time Factors
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D001973:Bromodeoxyuridine