title: Interaction between Neuronal Depolarization and MK-801 in SH-SY5Y Cells and the Rat Cortex.
abstract: OBJECTIVE
  The interaction between MK-801, a model of psychosis and KCl-induced depolarization or electroconvulsive shock (ECS), a therapeutic model of electroconvulsive therapy (ECT), was investigated in SH-SY5Y cells and the rat frontal cortex.
  
  
  METHODS
  SH-SY5Y cells were pretreated with 1 microM MK-801 for 15 min, followed by cotreatment with 100 mM KCl for 5 min. MK-801 was reintroduced after the KCl was washed out, and the samples were incubated before harvesting. For the experiments in rats, male Sprague-Dawley rats were treated with MK-801 followed by ECS. Immunoblot analyses of glycogen synthase kinase 3beta (GSK3beta) (Ser9), AKT (Ser473) and extracellular legulated kinase (ERK)1/2 in SH-SY5Y cells and the rat frontal cortex were performed.
  
  
  RESULTS
  KCl-induced neuronal depolarization resulted in the transient dephosphorylation of AKT (Ser473) and GSK3beta (Ser9), followed by increased phosphorylation of the enzymes in SH-SY5Y cells. Cotreatment with MK-801 and KCl inhibited the initial dephosphorylation of AKT and GSK3beta produced by KCl-induced neuronal depolarization. Similarly, ECS resulted in the transient dephosphorylation of AKT (Ser473) and GSK3beta (Ser9), whereas cotreatment with MK-801 inhibited the initial dephosphorylation of AKT (Ser473) and GSK3beta (Ser9) produced by ECS in the rat frontal cortex. No significant interaction was observed between MK-801 and KCl in the dephosphorylation of ERK1/2.
  
  
  CONCLUSIONS
  These results suggest that an antagonistic interplay between MK-801 and neuronal depolarization by KCl or ECS is involved the regulation of AKT (Ser473) and GSK3beta (Ser9) phosphorylation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Yeni
authors.initials: Y
authors.lastname: Kim
authors.forename: Miran
authors.initials: M
authors.lastname: Seo
authors.forename: Yun-Il
authors.initials: YI
authors.lastname: Lee
authors.forename: So-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Cho
authors.forename: Se-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Ahn
authors.forename: Ung-Gu
authors.initials: UG
authors.lastname: Kang
authors.forename: Yong-Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Juhnn