Stable recombinants of bovine viral diarrhea virus containing a hepatitis C virus insert.
Here we report on a segment in the genomic 3' non-translated region (3'NTR) of bovine viral diarrhea virus (BVDV) that is accessible for the insertion of foreign sequence elements such as the 5'NTR of hepatitis C virus. Recombinant viruses exhibited replication kinetics similar to those of the parental strain, and characterization of RNA species after several passages revealed that foreign inserts had the same genetic stability as the BVDV 3'NTR. The generation of such BVDV recombinants is relevant for several applications.
Baroth
Martina
M
Department of Microbial Biotechnology, Institute of Biochemistry and Biotechnology, Faculty of Life Sciences, Martin-Luther-University Halle-Wittenberg, Germany.
Peters
Yvonne
Y
Schönbrunner
E Ralf
ER
Behrens
Sven-Erik
SE
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D016174:Hepacivirus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010576:Pestivirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral