The soluble major histocompatibility complex class I-related chain A protein reduced NKG2D expression on natural killer and T cells from patients with prolactinoma and non-secreting pituitary adenoma.
This study analyses aspects of the immune system in prolactinoma and non-secreting pituitary adenoma. Serum soluble major histocompatibility complex class I-related chain A protein (sMICA) concentrations were measured by enzyme-linked immunosorbent assay. NKG2D-expressing natural killer and T cells were analyzed by flow cytometry. A correlation analysis was also performed to associate sMICA levels with NKG2D expression. The expression of MICA was examined in specific tissues by use of the reverse transcription-polymerase chain reaction. A significant amount of sMICA was detected in the serum of nearly all patients. We found decreased percentage and mean fluorescence intensity of NKG2D-expressing natural killer and T cells from patients with prolactinoma and non-secreting pituitary adenoma compared to those from healthy donors. Pearson analysis showed a negative correlation between sMICA and NKG2D-expressing cells. The immune-escape of pituitary adenoma is related to the down-regulation of NKG2D and the up-regulation of its ligand MICA.
Ma
Lixin
L
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.
Li
Gang
G
Su
Yuhang
Y
He
Qiaowei
Q
Zhang
Cai
C
Zhang
Jian
J
D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000097:blood* / Q000235:genetics; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000032:analysis / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000097:blood* / Q000235:genetics / Q000276:immunology; D015175:Prolactinoma / Q000097:blood* / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D019139:Tumor Escape / Q000502:physiology*; D015854:Up-Regulation; D055815:Young Adult
D015395:Histocompatibility Antigens Class I; C527085:KLRK1 protein, human; C099187:MHC class I-related chain A; D055655:NK Cell Lectin-Like Receptor Subfamily K