The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.
A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation during meiosis.
Lorenz
Alexander
A
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
West
Stephen C
SC
Whitby
Matthew C
MC
D004265:DNA Helicases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005816:Genetic Complementation Test; D045565:Holliday Junction Resolvases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; C495865:MUS81 protein, S pombe; D009153:Mutagens; D029702:Schizosaccharomyces pombe Proteins; D004720:Endonucleases; C533748:GEN1 protein, human; D045565:Holliday Junction Resolvases; D004265:DNA Helicases; C106462:Rqh1 protein, S pombe