Direct shoot regeneration from intact leaves of Arnebia euchroma (Royle) Johnston using thidiazuron.
The present study highlights the importance of preculture time and concentration of TDZ (thidiazuron) for direct regeneration from in vitro leaves (attached to shoots) in Arnebia euchroma. Shoot buds proliferated to form multiple shoots on MS medium (Murashige and Skoog medium) with 5.0 microM Kn. Different additives viz. ascorbic acid, PVP (polyvinylpyrrolidone), PVPP (polyvinylpolypyrrolidone) or activated charcoal (50, 100 and 250 mg/l each) were used to check the phenolic exudations. Direct shoot regeneration was obtained when shoots were initially precultured for 40 days on medium with a higher concentration of TDZ (20.0 muM) and then transferred to a lower concentration (5.0 microM TDZ). The identity of shoot buds was confirmed by histological studies. Regenerated shoots were cultured for 30 days on medium containing Kn (5.0 microM) for proliferation and then transferred to IBA (0.25 microM)-containing medium for rooting. Rooted plantlets were transferred to greenhouse with 45-50% survival.
Malik
Sonia
S
Division of Biotechnology, Institute of Himalayan Bioresource Technology CSIR, Palampur, HP, India.
Sharma
Shveta
S
Sharma
Madhu
M
Ahuja
Paramvir Singh
PS
D028082:Boraginaceae / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques / Q000379:methods*; D010636:Phenols / Q000378:metabolism; D010671:Phenylurea Compounds / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D010946:Plants, Medicinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D013830:Thiadiazoles / Q000494:pharmacology*
D010636:Phenols; D010671:Phenylurea Compounds; D013830:Thiadiazoles; C016785:thidiazuron