title: P-selectin expression in cold preserved kidneys in University of Wisconsin and histidine-tryptophan-ketoglutarate solutions.
abstract: The differences and efficacy of standard preservation solutions in kidney transplantation, University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK), remain a topic of debate in recent clinical studies. P-selectins represent glycoproteins expressed on endothelial cells and platelets responsible for the earliest events in ischemia/reperfusion injury in kidney transplantation. This study aimed to compare the levels of P-selectin expression between cold preserved kidney tissues in UW and HTK solutions. Thirty kidneys were procured from male Lewis rats and stored in cold (4°C) solutions for periods of 4, 12, 16, 20, and 24h. Group 1 (n=15) kidneys were stored in UW solutions, and group 2 (n=15) kidneys were submerged in HTK solutions. At the end of each time point, the kidneys underwent preparation and levels of P-selectin expression in the tissues were measured using Immunoblot analyses and adjusted volumetric quantification of Western blot signals. For all periods of cold preservation, P-selectin expression was significantly down-regulated in kidney tissues stored in UW compared with HTK solutions (P<0.001). In summary, UW demonstrated a significant benefit over HTK solution in down-regulating P-selectin expression in cold preserved kidney grafts.
authors.affiliation: Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA. dworku@tulane.edu
authors.forename: Dawit
authors.initials: D
authors.lastname: Worku
authors.forename: Sebastian
authors.initials: S
authors.lastname: Laluf
authors.forename: Jennifer
authors.initials: J
authors.lastname: McGee
authors.forename: Monica
authors.initials: M
authors.lastname: Goswami
authors.forename: Keith
authors.initials: K
authors.lastname: VanMeter
authors.forename: Douglas P
authors.initials: DP
authors.lastname: Slakey
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D015536:Down-Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D023421:Models, Animal; D009336:Necrosis / Q000378:metabolism / Q000473:pathology; D019188:Organ Preservation Solutions / Q000494:pharmacology; D019007:P-Selectin / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; D019007:P-Selectin; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose