Multivalent DNA vaccine enhanced protection efficacy against infectious bronchitis virus in chickens.
For efficacious DNA vaccine development against infectious bronchitis virus (IBV), the immunogenicity of a multivalent DNA vaccine was evaluated. Three expression plasmids each targeting spike protein (S1), nucleocapsid protein (N), and membrane protein (M) of IBV were prepared. Chickens were immunized with either individual plasmids (monovalent) or with a combination of all plasmids (multivalent). Immunization with the multivalent DNA vaccine induced synergistic augmentation of humoral and cellular responses in comparison with the individual vaccines, and provided up to 85% immune protection. Thus the multivalent DNA vaccine represents an innovative approach for enhancing DNA vaccine potency, and has potential clinical application for vaccination against IBV.
Yang
Tai
T
Animal Disease Prevention and Food Safety Key Lab of Sichuan Province, School of Life Sciences, Sichuan University, Chengdu, 610064, People's Republic of China.
Wang
Hong-Ning
HN
Wang
Xue
X
Tang
Jun-Ni
JN
Gao
Rong
R
Li
Juan
J
Guo
Zi-Cheng
ZC
Li
Yu-Ling
YL
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002645:Chickens; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000517:prevention & control / Q000662:veterinary*; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D019444:Vaccines, DNA / Q000276:immunology*; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*
D000956:Antigens, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines