Influence of pressure on the retention of sugars in hydrophilic interaction chromatography.
Pressure can influence the retention of analytes in hydrophilic interaction chromatography as well as in RP chromatography. We demonstrate that the retention of sugars in hydrophilic interaction chromatography decreases with pressure, and interpret the observation as a gain in solvation, or more specifically hydration, as the sugar molecules enter the water-rich stationary phase.
Neue
Uwe D
UD
Waters Corporation, Milford, MA 01721, USA. Uwe_Neue@Waters.com
Hudalla
Chris J
CJ
Iraneta
Pamela C
PC