title: Immunoglobulin gene locus events in epithelial cells of lactating mouse mammary glands.
abstract: It has been believed that the immunoglobulin (Ig) found abundantly in the colostrum of lactating mammalian is derived from serum or secreted by plasma cells present in the mammary gland. The recent finding of Ig gene rearrangements in breast cancer cells and benign hyperplastic breast epithelial cells suggests that it is likely that hyperplastic mammary gland epithelial cells during lactation can also produce Ig. In this study, we have demonstrated the presence of abundant amounts of Ig heavy and light chain transcripts in sorted cytokeratin 18-positive mammary gland epithelial cells of lactating mice. Interestingly, we found two specific Igkappa variable region sequences (V(CW9)J(kappa1) and V(BV9)J(kappa1)) that were dominantly expressed in different strains of mice. Our data demonstrate that IgG is expressed by mammary gland epithelial cells of lactating mice, and suggest that the IgG found in murine colostrum is at least partially produced by the mammary gland epithelial cells.
authors.affiliation: Center for Human Disease Genomics, Peking University, Haidian District, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Mao
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Lemeng
authors.initials: L
authors.lastname: Wu
authors.forename: C Cameron
authors.initials: CC
authors.lastname: Yin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains