A Streptococcus pyogenes derived collagen-like protein as a non-cytotoxic and non-immunogenic cross-linkable biomaterial.
A range of bacteria have been shown to contain collagen-like sequences that form triple-helical structures. Some of these proteins have been shown to form triple-helical motifs that are stable around body temperature without the inclusion of hydroxyproline or other secondary modifications to the protein sequence. This makes these collagen-like proteins particularly suitable for recombinant production as only a single gene product and no additional enzyme needs to be expressed. In the present study, we have examined the cytotoxicity and immunogenicity of the collagen-like domain from Streptococcus pyogenes Scl2 protein. These data show that the purified, recombinant collagen-like protein is not cytotoxic to fibroblasts and does not elicit an immune response in SJL/J and Arc mice. The freeze dried protein can be stabilised by glutaraldehyde cross-linking giving a material that is stable at >37 degrees C and which supports cell attachment while not causing loss of viability. These data suggest that bacterial collagen-like proteins, which can be modified to include specific functional domains, could be a useful material for medical applications and as a scaffold for tissue engineering.
Peng
Yong Y
YY
CSIRO Molecular and Health Technologies, Bayview Avenue, Clayton, Victoria 3169, Australia.
Yoshizumi
Ayumi
A
Danon
Stephen J
SJ
Glattauer
Veronica
V
Prokopenko
Olga
O
Mirochnitchenko
Oleg
O
Yu
Zhuoxin
Z
Inouye
Masayori
M
Werkmeister
Jerome A
JA
Brodsky
Barbara
B
Ramshaw
John A M
JA
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005976:Glutaral / Q000494:pharmacology; D007114:Immunization; D051379:Mice; D055550:Protein Stability / Q000187:drug effects; D017434:Protein Structure, Tertiary; D012995:Solubility / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000378:metabolism*
D001426:Bacterial Proteins; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; C423453:Scl2 protein, Streptococcus; D003094:Collagen; D005976:Glutaral