A validated assay to measure soluble IL-7 receptor shows minimal impact of IL-7 treatment.
IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is important to develop a sensitive and reliable assay in order to investigate the potential role of this receptor in the regulation of IL-7 physiologic, physipathologic and therapeutic effects. We here report a fully validated method to measure sCD127 in human and simian plasma using a method based on ELISA MSD technology. We demonstrate that sCD127 is detectable at various levels in the plasma of healthy humans as well as in that of healthy Rhesus and Cynomolgus macaques (106.72, 205.26 and 366.95 ng/mL respectively). Moreover, as opposed to the sCD25/IL-2 tandem, we demonstrate that IL-7 treatment has no impact on sCD127 plasma concentration in patients infected by HIV.
Janot-Sardet
Caroline
C
Cytheris S.A., Technopolis, 175 rue J.J. Rousseau, 92130 Issy les Moulineaux, France.
Assouline
Brigitte
B
Cheynier
Rémi
R
Morre
Michel
M
Beq
Stéphanie
S
D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D015851:Interleukin-7 / Q000627:therapeutic use*; D053699:Interleukin-7 Receptor alpha Subunit / Q000097:blood*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D011994:Recombinant Proteins / Q000627:therapeutic use; D015203:Reproducibility of Results; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*
D000998:Antiviral Agents; C508605:IL7 protein, human; D015851:Interleukin-7; D053699:Interleukin-7 Receptor alpha Subunit; D011994:Recombinant Proteins