Fate specification and tissue-specific cell cycle control of the Caenorhabditis elegans intestine.
Coordination between cell fate specification and cell cycle control in multicellular organisms is essential to regulate cell numbers in tissues and organs during development, and its failure may lead to oncogenesis. In mammalian cells, as part of a general cell cycle checkpoint mechanism, the F-box protein beta-transducin repeat-containing protein (beta-TrCP) and the Skp1/Cul1/F-box complex control the periodic cell cycle fluctuations in abundance of the CDC25A and B phosphatases. Here, we find that the Caenorhabditis elegans beta-TrCP orthologue LIN-23 regulates a progressive decline of CDC-25.1 abundance over several embryonic cell cycles and specifies cell number of one tissue, the embryonic intestine. The negative regulation of CDC-25.1 abundance by LIN-23 may be developmentally controlled because CDC-25.1 accumulates over time within the developing germline, where LIN-23 is also present. Concurrent with the destabilization of CDC-25.1, LIN-23 displays a spatially dynamic behavior in the embryo, periodically entering a nuclear compartment where CDC-25.1 is abundant.
Segref
Alexandra
A
Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
Cabello
Juan
J
Clucas
Caroline
C
Schnabel
Ralf
R
Johnstone
Iain L
IL
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D044783:F-Box Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry / Q000379:methods; D007422:Intestines / Q000166:cytology*; D018613:Microscopy, Confocal / Q000379:methods; D008954:Models, Biological; D008957:Models, Genetic; D010641:Phenotype; D034622:RNA Interference; D020687:cdc25 Phosphatases / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; C418507:lin-23 protein, C elegans; D020687:cdc25 Phosphatases