High-throughput caveolar proteomic signature profile for maternal binge alcohol consumption.
Currently, no single marker is sensitive and specific enough to be considered a reliable biomarker for prenatal alcohol exposure. To identify a proteomic signature profile for maternal alcohol consumption, we carried out high-throughput proteomics on maternal endothelial caveolae exposed to moderate binge-like alcohol conditions. In these specialized lipid-ordered microdomains that contain a rich assembly of proteins, we demonstrate that moderate binge-like alcohol resulted in a distinctive maternal caveolar proteomic signature with important proteins being dramatically decreased/knocked out in the alcoholic profile. These proteins span from histones and basic structural proteins like α tubulin to proteins involved in trafficking, deubiquitination, cell signaling, and cell-cell adhesion. The profile also suggests an important role for the mother and the uteroplacental compartment in the pathogenesis of fetal alcohol spectrum disorders (FASD). These data demonstrate that the caveolar proteomic signature created by alcohol shows a promising direction for early detection of FASD.
Ramadoss
Jayanth
J
Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin, Atrium-B Meriter Hospital, 202 South Park Street Madison, WI 53715, USA. ramadoss@wisc.edu
Liao
Wu-xiang
WX
Chen
Dong-bao
DB
Magness
Ronald R
RR
D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D021941:Caveolae / Q000737:chemistry*; D051242:Caveolin 1 / Q000032:analysis; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000737:chemistry / Q000648:ultrastructure*; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000175:diagnosis; D052250:Nitric Oxide Synthase Type III / Q000032:analysis; D011247:Pregnancy; D040901:Proteomics; D012756:Sheep; D015398:Signal Transduction / Q000235:genetics; D014404:Tubulin / Q000032:analysis
D002352:Carrier Proteins; D051242:Caveolin 1; D014404:Tubulin; D000431:Ethanol; D052250:Nitric Oxide Synthase Type III