Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains.
Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of S. cerevisiae.
Coulon
Joël
J
LCPME, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Nancy-University, CNRS, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, France. joel.coulon@pharma.uhp-nancy.fr
Thouvenin
Ilan
I
Aldeek
Fadi
F
Balan
Lavinia
L
Schneider
Raphaël
R
D019187:Cadmium Compounds / Q000737:chemistry; D005453:Fluorescence; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006031:Glycosylation; D007716:Kluyveromyces / Q000737:chemistry / Q000302:isolation & purification*; D037102:Lectins / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009172:Mycology / Q000379:methods*; D045663:Quantum Dots; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000302:isolation & purification*; D013691:Tellurium / Q000737:chemistry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D019187:Cadmium Compounds; D037102:Lectins; D014867:Water; D005947:Glucose; D013691:Tellurium; D008358:Mannose; C028337:cadmium telluride; D005690:Galactose