Characterization of endocytic compartments after holo-high density lipoprotein particle uptake in HepG2 cells.
Holo-high density lipoprotein (HDL) particle uptake, besides selective lipid uptake, constitutes an alternative pathway to regulate cellular cholesterol homeostasis. In the current study, the cellular path of holo-HDL particles was investigated in human liver carcinoma cells (HepG2) using combined light and electron microscopical methods. The apolipoprotein moiety of HDL was visualized with different markers: horseradish peroxidase, colloidal gold and the fluorochrome Alexa(568), used in fluorescence microscopy and after photooxidation correlatively at the ultrastructural level. Time course experiments showed a rapid uptake of holo-HDL particles, an accumulation in endosomal compartments, with a plateau after 1-2 h of continuous uptake, and a clearance 1-2 h upon replacement by unlabeled HDL. Correlative microscopy, using HDL-Alexa(568)-driven diaminobenzidine (DAB) photooxidation, identified the fluorescent organelles as DAB-positive multivesicular bodies (MVBs) in the electron microscope; their luminal contents but not the internal vesicles were stained. Labeled MVBs increased in numbers and changed shapes along with the duration of uptake, from polymorphic organelles with multiple surface domains and differently shaped protrusions dominating at early times of uptake to compact bodies with mainly tubular appendices and densely packed vesicles after later times. Differently shaped and labeled surface domains and appendices, as revealed by three dimensional reconstructions, as well as images of homotypic fusions indicate the dynamics of the HDL-positive MVBs. Double staining visualized by confocal microscopy, along with the electron microscopic data, shows that holo-HDL particles after temporal storage in MVBs are only to a minor degree transported to lysosomes, which suggests that different mechanisms are involved in cellular HDL clearance, including resecretion.
Röhrl
Clemens
C
Center for Physiology and Pathophysiology, Institute of Medical Chemistry, Medical University of Vienna, Vienna, Austria.
Pagler
Tamara A
TA
Strobl
Witta
W
Ellinger
Adolf
A
Neumüller
Josef
J
Pavelka
Margit
M
Stangl
Herbert
H
Meisslitzer-Ruppitsch
Claudia
C
D004705:Endocytosis; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D056945:Hep G2 Cells; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size
D008075:Lipoproteins, HDL; D006046:Gold; D006735:Horseradish Peroxidase