Effect of local hyperthermia on lymphangiogenic factors VEGF-C and -D in a nude mouse xenograft model of tongue squamous cell carcinoma.
It is widely accepted that tumor-induced lymphangiogenesis driven by vascular endothelial growth factor (VEGF)-C- and/or VEGF-D-induced activation of VEGF receptor (VEGFR)-3 could promote lymphatic metastasis. In this study, tumor growth and intratumoral expression level of VEGF-C and -D following administration of local hyperthermia was evaluated in nude mice model of tongue squamous cell carcinoma (SCC). The data demonstrated that the size of tumor in local hyperthermia was 26.5% of control group, and that local hyperthermia markedly suppressed the mRNA and protein expression of VEGF-C and -D as determined by quantitative real-time RT-PCR, Western blot and immunohistochemistry (P<0.05). These results suggest that, accompanying with tumor growth inhibition, local hyperthermia may act as an anti-lymphangiogenic role by suppressing the expression of tumor VEGF-C and -D, and thereby inhibiting cancer cell lymphatic metastasis in tongue SCC.
Liang
Xinhua
X
State Key Laboratory of Oral Diseases, Sichuan University, No. 14, Sec. 3, Renminnan Road, Chengdu, Sichuan 610041, People's Republic of China. lxh88866@scu.edu.cn
Zhou
Hao
H
Liu
Xian
X
He
Yongwen
Y
Tang
Yaling
Y
Zhu
Guiquan
G
Zheng
Min
M
Yang
Jing
J
D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006979:Hyperthermia, Induced / Q000379:methods; D042583:Lymphangiogenesis / Q000502:physiology*; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D014183:Transplantation, Heterologous; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*; D042643:Vascular Endothelial Growth Factor D / Q000378:metabolism*
D042582:Vascular Endothelial Growth Factor C; D042643:Vascular Endothelial Growth Factor D; D040262:Receptors, Vascular Endothelial Growth Factor