title: Isolation, characterization and phylogenetic analysis of a bacterial strain capable of degrading acetamiprid.
abstract: An aerobic bacterium, capable of degrading the new chloronicotine pesticide acetamiprid, was isolated from the sludge of pesticide factory after successive enrichment cultures and named strain FH2 which is a Gram-negative, rod-shaped, obligate aerobic organism with ((0.5-0.7) x (1.5-3.0)) microm of cell size and with monotrichous flagellum. It was identified as a member of Pseudomonas sp. based on morphology, physio-biochemical properties, Biolog GN2, 16S rDNA sequence and phylogenetic characteristic analysis. The isolate could grow optimally at pH 7.0 and 30 degrees C in acetamiprid-mineral medium with 800 mg/L concentration. About 53.3% acetamiprid was degraded by strain FH2 after incubation for 14 d in acetamiprid-mineral medium and nearly 96.7% degraded when incubated in acetamiprid-yeast mineral medium at 30 degrees C for 14 d. This paper describes phylogenetic and degradation characterization of a pure bacterium being able to mineralize acetamiprid for the first time.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310029, China. xhyao2003@sohu.com
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Yao
authors.forename: Hang
authors.initials: H
authors.lastname: Min
mesh_terms: D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D000073943:Neonicotinoids; D010575:Pesticides / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal; D000073943:Neonicotinoids; D010575:Pesticides; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; C464485:acetamiprid