title: Analyzing habituation responses to novelty in zebrafish (Danio rerio).
abstract: Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modulation. Although zebrafish (Danio rerio) are increasingly utilized in neurobehavioral research, their habituation responses have not been extensively investigated. Utilizing the novel tank test, we examine intra- and inter-session habituation and demonstrate robust habituation responses in adult zebrafish. Analyzing the intra-session habituation to novelty further, we also show that selected anxiogenic drugs (caffeine, pentylenetetrazole), as well as stress-inducing alarm pheromone, attenuated zebrafish habituation. Some acute anxiolytic agents, such as morphine and ethanol, while predictably reducing zebrafish anxiety, had no effects on habituation. Chronic ethanol and fluoxetine treatments improved intra-session habituation in zebrafish. In general, our study parallels literature on rodent habituation responses to novelty, and reconfirms zebrafish as a promising model for cognitive neurobehavioral research.
authors.affiliation: Department of Pharmacology, Tulane University Medical School, New Orleans, LA 70112, USA.
authors.forename: Keith
authors.initials: K
authors.lastname: Wong
authors.forename: Marco
authors.initials: M
authors.lastname: Elegante
authors.forename: Brett
authors.initials: B
authors.lastname: Bartels
authors.forename: Salem
authors.initials: S
authors.lastname: Elkhayat
authors.forename: David
authors.initials: D
authors.lastname: Tien
authors.forename: Sudipta
authors.initials: S
authors.lastname: Roy
authors.forename: Jason
authors.initials: J
authors.lastname: Goodspeed
authors.forename: Chris
authors.initials: C
authors.lastname: Suciu
authors.forename: Julia
authors.initials: J
authors.lastname: Tan
authors.forename: Chelsea
authors.initials: C
authors.lastname: Grimes
authors.forename: Amanda
authors.initials: A
authors.lastname: Chung
authors.forename: Michael
authors.initials: M
authors.lastname: Rosenberg
authors.forename: Siddharth
authors.initials: S
authors.lastname: Gaikwad
authors.forename: Ashley
authors.initials: A
authors.lastname: Denmark
authors.forename: Andrew
authors.initials: A
authors.lastname: Jackson
authors.forename: Ferdous
authors.initials: F
authors.lastname: Kadri
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Chung
authors.forename: Adam
authors.initials: A
authors.lastname: Stewart
authors.forename: Tom
authors.initials: T
authors.lastname: Gilder
authors.forename: Esther
authors.initials: E
authors.lastname: Beeson
authors.forename: Ivan
authors.initials: I
authors.lastname: Zapolsky
authors.forename: Nadine
authors.initials: N
authors.lastname: Wu
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cachat
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006185:Habituation, Psychophysiologic / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018709:Statistics, Nonparametric; D015027:Zebrafish / Q000502:physiology*
chemical_list: D014151:Anti-Anxiety Agents