title: Fluorescent analysis of excess electron transfer through DNA.
abstract: The DNA base stack provides unique features for the efficient long-range charge transfer. For the purpose of investigating excess electron transfer process through DNA, we developed a new method for fluorescence analysis of excess electron transfer based on reductive cleavage of a disulfide bond and a thiol-specific fluorescent probe. Excess electron transfer was detected by monitoring the fluorescence of emissive pyrene monomer generated by the reaction of pyrene maleimides with the cleaved disulfide bond (thiols). Mechanism of reductive cleavage of disulfides through excess electron transfer and subsequent reaction with the fluorescent probes were discussed. This facile and sensitive detection by fluorescence method can be applied for mechanistic study of excess electron transfer.
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan. takada@eng.u-hyogo.ac.jp
authors.forename: Tadao
authors.initials: T
authors.lastname: Takada
authors.forename: Chie
authors.initials: C
authors.lastname: Tanaka
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yamana
mesh_terms: D004247:DNA / Q000032:analysis* / Q000502:physiology*; D004579:Electron Transport / Q000502:physiology*; D005456:Fluorescent Dyes / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D004247:DNA