[Comparison between two methods for the measurement reticulated platelet and their clinical significance--flow cytometry (FCM) method and IPF method Using automated hematology analyzer (XE-2000)].
Reticulated platelet (RP) is thought to be a useful marker for differential diagnosis and analysis of platelet kinetics in patients with thrombocytopenia. In this study, we compared two methods for the measurement of RP: flow cytometric (FCM) method and immature platelet fraction (IPF) method using automated hematology analyzer (XE-2000). There was a relatively good correlation between RP% measured by FCM method and IPF% measured by IPF method in patients with idiopathic thrombocytopenic purpura (ITP) (Y = 0.806X-0.050, r = 0.634, p < 0.001). We then measured RP% and IPF% in 61 patients with ITP and 27 patients with aplastic anemia (AA). For the differential diagnosis for ITP, the sensitivity (82%) and specificity (93%) of FCM method were better than those of IPF method (sensitivity 67% and specificity 63%). Our data demonstrate the significant difference between two methods by analyzing clinical samples in parallel.
Hayashi
Satoru
S
Department of Blood Transfusion, Osaka University Hospital, Suita 565-0879, Japan. hayashi@hp-blood.med.osaka-u.ac.jp
Nishiyama
Miho
M
Suehisa
Etsuji
E
Kashiwagi
Hirokazu
H
Kurata
Yoshiyuki
Y
Tomiyama
Yoshiaki
Y
D000328:Adult; D001331:Automation; D001792:Blood Platelets / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count / Q000379:methods*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis; D012680:Sensitivity and Specificity; D013921:Thrombocytopenia / Q000175:diagnosis