title: Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid.
abstract: The objective was to investigate the effects of oxygen tension and follicle cells (FCs) during in vitro maturation of porcine oocytes in only porcine (Sus scrofa domesticus) follicular fluid (pFF), using static and non-static (rotating) culture systems, on the nuclear maturation and subsequent in vitro fertilization of the oocytes. In the first experiment, cumulus-oocyte complexes (COCs) were matured for 48 h in pFF supplemented with (+) or without (-) FCs (5.2x10(6) cells/mL), using the static (S) and rotating (R) culture systems (+FC/S, -FC/S, +FC/R, and -FC/R) under 5% or 20% O(2). Co-culture with FCs in the static culture system (+FC/S) had a detrimental effect on the meiotic competence of oocytes, whereas co-culture with FCs in the rotating culture system (+FC/R) increased maturation rates. In both culture systems, oxygen tension had no apparent effects on meiotic competence of oocytes, irrespective of culture system and FC addition. In the second experiment, COCs were matured under 5% or 20% O(2) using the -FC/S or +FC/R culture systems and then fertilized. Oxygen tension had no significant effects on fertilization parameters, irrespective of the culture system. The rotating culture system increased rates of sperm penetration and male pronuclear formation and decreased polyspermic fertilization compared with the static culture system (P < 0.05). In conclusion, both -FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the -FC/S culture system for promoting fertilization.
authors.affiliation: National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Agung
authors.forename: Y
authors.initials: Y
authors.lastname: Piao
authors.forename: D
authors.initials: D
authors.lastname: Fuchimoto
authors.forename: S
authors.initials: S
authors.lastname: Senbon
authors.forename: A
authors.initials: A
authors.lastname: Onishi
authors.forename: T
authors.initials: T
authors.lastname: Otoi
authors.forename: T
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000662:veterinary; D003470:Culture Media; D054885:Cumulus Cells / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D015571:Follicular Fluid; D008540:Meiosis; D009865:Oocytes / Q000254:growth & development / Q000502:physiology*; D010100:Oxygen / Q000008:administration & dosage*; D013552:Swine
chemical_list: D003470:Culture Media; D010100:Oxygen