Intra-ocular expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in a case of Eales' disease by immunohistochemical analysis: a case report.
We report a immunohistochemical study of expression of VEGF and PEDF in Eales' disease. An enucleated eye from a patient with Eales' disease and an age and sex-matched donor eyeball were subjected to immunohistochemical and histopathological analysis to study the expression of VEGF and PEDF in the retinal region. Strong positive anti-VEGF immunostaining was found around the vessel walls in the retina in the eyeball from the Eales' disease patient compared with the donor control, from which it was almost absent. PEDF staining was detected in the normal donor eyeball retinal layers surrounding the blood vessels, and the photoreceptor and ganglion cell layer, whereas in the eyeball from Eales' disease patient PEDF staining was restricted to the surrounding blood vessels. The strong expression of VEGF in eyes with Eales' disease as opposed to weak expression of PEDF indicates the propensity of the disease to show neovascularization and recurrent hemorrhages.
Verma
Aditya
A
Department of Ocular Pathology, Medical Research Foundation, Sankara Nethralaya, Chennai, 600-006, India. dradi27@gmail.com
Biswas
Jyotirmay
J
Radhakrishnan
Selvi
S
Narayanasamy
Angayarkanni
A
D015353:Eye Enucleation; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000378:metabolism*; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D012171:Retinal Vessels; D015843:Serpins / Q000378:metabolism*; D013194:Staining and Labeling; D014018:Tissue Distribution; D014019:Tissue Donors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014657:Vasculitis / Q000150:complications*; D014823:Vitreous Hemorrhage / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor