A storage protein-like Trypsin inhibitor from the Moth Bean (Phaseolus acutifolius) with antiproliferative activity toward lymphoma cells.
A 26-kDa trypsin inhibitor with an N-terminal sequence resembling storage proteins was purified from moth beans (Phaseolus acutifolius). It dose-dependently inhibited trypsin with an IC(50) value of about 0.38 microM. It inhibited [methyl-(3)H] thymidine incorporation by lymphoma MBL2 cells with an IC(50) value of 20 microM.
Ma
D Z
DZ
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
Sun
J
J
Zhang
G Q
GQ
Wang
H X
HX
Ng
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D020171:Botrytis / Q000187:drug effects / Q000254:growth & development; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D027805:Phaseolus / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D000935:Antifungal Agents; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D014361:Trypsin Inhibitors; D014316:Tritium; D054303:HIV Reverse Transcriptase; D014357:Trypsin; D013936:Thymidine