Novel fructopyranose oligosaccharides isolated from fermented beverage of plant extract.
Four oligosaccharides containing a fructopyranosyl residue have been found from fermented beverage of plant extract and isolated from the beverage using carbon-Celite column chromatography and preparative high performance liquid chromatography. Structure confirmation of the saccharides was provided by methylation analysis, MALDI-TOF-MS and NMR measurements. These saccharides were identified as oligosaccharides of fructopyranoside series; beta-D-fructopyranosyl-(2-->6)-D-fructofuranose (1), beta-D-fructopyranosyl-(2-->1)-D-fructopyranose (2), beta-D-fructopyranosyl-(2-->1)-beta-D-fructofuranosyl-(2<-->1)-alpha-D-glucopyranoside (3), and beta-D-fructopyranosyl-(2-->6)-alpha-D-glucopyranosyl-(1<-->2)-beta-D-fructofuranoside (4). Saccharides 3 and 4 among novel saccharides 1, 3, and 4 were named 'pyrano-1-kestose (pyrano-isokestose)' and 'pyrano-neokestose', respectively.
Okada
Hideki
H
General Institute of Ohtakakohso, Co., Ltd, Otaru 047-0193, Japan.
Fukushi
Eri
E
Yamamori
Akira
A
Kawazoe
Naoki
N
Onodera
Shuichi
S
Kawabata
Jun
J
Shiomi
Norio
N
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; D010936:Plant Extracts