Effect of chondroitinase on dermatan sulfate-facilitated arginine amidase released from rabbit ear artery.
We examined the effects of chondroitinases on the release of dermatan sulfate (DS)-induced arginine amidase (AA) from rabbit ear artery. DS-induced AA release was significantly decreased by treatment with chondroitinase ABC (ABCase) in the rabbit ear artery. On the other hand, Chondroitinase ACII (ACIIase) enhanced spontaneous and DS-induced AA release. Heat-inactivated ABCase and ACIIase did not affect spontaneous and DS-induced AA release. Furthermore, ABCase, but not ACIIase and heat-inactivated chondroitinases, degraded DS. These results indicate that the facilitatory effect of DS-induced AA release from the rabbit ear artery is affected by the molecular size of DS.
Ishii-Nozawa
Reiko
R
Department of Clinical Pharmacology, Meiji Pharmaceutical University, Tokyo, Japan. reiko-in@my-pharm.ac.jp
Naito
Tatsuya
T
Mita
Mitsuo
M
Miyazaki
Kyosuke
K
Matsuda
Yoshifumi
Y
Takeuchi
Koichi
K
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001158:Arteries / Q000187:drug effects* / Q000378:metabolism; D019765:Chondroitin ABC Lyase / Q000494:pharmacology; D002808:Chondroitin Lyases / Q000494:pharmacology*; D003871:Dermatan Sulfate / Q000737:chemistry / Q000378:metabolism*; D004423:Ear; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism*; D013329:Structure-Activity Relationship
D000925:Anticoagulants; D003871:Dermatan Sulfate; D012697:Serine Endopeptidases; C402942:arginine amidase; D002808:Chondroitin Lyases; D019765:Chondroitin ABC Lyase