A novel high molecular weight glutenin subunit from Australopyrum retrofractum.
We describe the sequence of a gene encoding a high molecular weight glutenin subunit (HMW-GS) expressed in the endosperm of the wheat relative Australopyrum retrofractum. Although the subunit has a similar primary structure to that HMW-GS genes present in other Triticeae species, its N-terminal domain is shorter, its central repetitive domain includes a unique dodecameric motif, and its C-terminal domain contain an extra cysteine residue. A phylogenetic analysis showed that the Glu-W1 gene is neither a true x- nor a true y-type subunit, although it is more closely related to the y-type genes present in the K and E genomes than to any other published HMW-GS gene. All these results indicated that this novel subunit may undergo a special evolutionary process different from other Triticeae species. A flour supplementation experiment showed that the Glu-W1 subunit has a negative effect on dough quality, which might be the result of interaction between the two closely placed cysteine residues in the C-terminal region.
Liu
Shuwei
S
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, 250100, Jinan, People's Republic of China.
Zhao
Feng
F
Gao
Xin
X
Chen
Fanguo
F
Xia
Guangmin
G
D000595:Amino Acid Sequence; D056625:Endosperm; D005983:Glutens / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D006109:Poaceae / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment
D021122:Protein Subunits; D005983:Glutens; C037302:glutenin