title: Metallic Nanostructures as Localized Plasmon Resonance Enhanced Scattering Probes for Multiplex Dark Field Targeted Imaging of Cancer Cells.
abstract: In this paper, we report the use of bioconjugated gold nanorods and silver nanoparticles as targeted localized surface plasmon resonance enhanced scattering probes for dark field multiplex and transmission electron microscopy (TEM) imaging of pancreatic cancer cells. We take advantage of the spectrally widely separated localized plasmon resonance of the gold nanorods and silver nanoparticles which produce wavelength selective plasmon resonance scattering to allow multiplex imaging with high contrast. By functionalizing the surfaces, aqueous dispersions of bioconjugated gold nanorods and silver nanoparticles are prepared. We demonstrate receptor-mediated delivery of bioconjugated gold nanorods and silver nanoparticles simultaneously into pancreatic cancer cells, using multiplexed dark field microscopy technique. We also show that the bioconjugated metallic nanostructures can be used for high contrast TEM imaging as well.
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, University at Buffalo, The State University of New York, Buffalo, New York 14260-4200, and Centre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
authors.forename: Indrajit
authors.initials: I
authors.lastname: Roy
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
authors.forename: Sailing
authors.initials: S
authors.lastname: He
authors.forename: Paras N
authors.initials: PN
authors.lastname: Prasad