title: Value of amniotic fluid interleukin-8 for the prediction of histological chorioamnionitis in preterm premature rupture of membranes.
abstract: OBJECTIVE
  To determine whether amniotic fluid levels of interleukin-8 (IL-8) are of value in the antenatal diagnosis of acute histological chorioamnionitis (HCA) in preterm premature rupture of membranes (PPROM).
  
  
  METHODS
  Department of Obstetrics and Gynaecology, Charles University, Medical School and University Hradec Kralove, Czech Republic.
  
  
  METHODS
  We compared amniotic fluid IL-8 levels in twenty-nine pregnant women with preterm premature rupture of membranes between 24th and 36th gestational weeks with presence and absence acute histological chorioamnionitis or/and microbial invasion in the amniotic cavity using nonparametric tests (Mann-Whitney test), given the non-normal distribution of analyte. Comparisons of proportions were performed with Shapiro-Wilk normality test.
  
  
  RESULTS
  Patients with HCA had a significantly higher median amniotic fluid IL-8 concentration than patients without the histological signs of chorioamnionitis (1867 pg/mL, 826-5577 versus 1045 pg/mL, 60-4133, p=0.013). Patients with MIAC had a significantly higher median amniotic fluid level than patients without invasion (1888 pg/mL, 519-5577 versus 1225 pg/mL, 60-2766, p= 0.017). Women with HCA and MIAC had a significantly higher median amniotic fluid IL-8 level than women without histological signs of chorioamnionitis and microbial invasion (3117 pg/mL, 826-5577 versus 1468 pg/mL, 394-2766, p=0.034).
  
  
  CONCLUSIONS
  HCA or/and MIAC are associated with a significant increase of amniotic fluid interleukin-8 levels. Amniotic fluid IL-8 seems to be a marker of intraamniotic inflammation.
authors.affiliation: Department of Obstetrics and Gynaecology, Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. kacermar@fnhk.cz
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
authors.forename: Marcela
authors.initials: M
authors.lastname: Drahosova
authors.forename: Helena
authors.initials: H
authors.lastname: Hornychova
authors.forename: Lenka
authors.initials: L
authors.lastname: Pliskova
authors.forename: Radka
authors.initials: R
authors.lastname: Bolehovska
authors.forename: Miroslav
authors.initials: M
authors.lastname: Forstl
authors.forename: Jindrich
authors.initials: J
authors.lastname: Tosner
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism* / Q000382:microbiology; D015415:Biomarkers / Q000378:metabolism; D002821:Chorioamnionitis / Q000175:diagnosis* / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000378:metabolism* / Q000382:microbiology; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016209:Interleukin-8 / Q000378:metabolism*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011446:Prospective Studies; D014509:Ureaplasma / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016209:Interleukin-8