[Effect of Dahuangzhechong pill on the gene expression spectra of preventing arterial thrombosis].
OBJECTIVE
To investigate the effect of Dahuangzhechong pill on the gene expression spectra of preventing arterial thrombosis, and reveal its mechanism on molecule level.


METHODS
Mononuclear cell and blood platelet of the arterial thrombosis patients were separated before and after treatment by Dahuangzhechong pill. Their RNA was extracted respectively and the genes expressions were detected using gene array containing 14,000 gene.


RESULTS
44 genes up-expressed and 299 genes down-expressed in blood platelet, 252 genes expression increased and 299 genes expression decreased in mononuclear cell genes after treated with Dahuangzhechong pill. The cluster analysis showed that the genes contained ion channel and transport protein, apoptosis related protein, DNA synthesis, repair and transcription factor, cell receptor, cell signal and transducin, and protein translation and synthesis, etc.


CONCLUSIONS
Dahuangzhechong pill may prevent arterial thrombosis through genes containing ion channel and transport protein, apoptosis related protein, DNA synthesis, repair and transcription factor, cell receptor, cell signal and transducin, and protein translation and synthesis, etc.
Wang
Dong-sheng
DS
Department of Integrated Chinese and Western Medicine, Xiangya Hospital/The Technology and Engineering Center of Traditional Chinese Medicine Selection/Postdoctor Workshop of Material and Engineering, Central South University, Changsha 410008, China. wdsh66@yahoo.com.cn
Chen
Fang-ping
FP
He
Shi-lin
SL
Tang
Fa-qing
FQ
D001158:Arteries; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D008419:Materia Medica / Q000494:pharmacology* / Q000627:therapeutic use; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013927:Thrombosis / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*
D017931:DNA Primers; D018076:DNA, Complementary; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica