title: Chemoenzymatic conversion of linoleic acid into conjugated linoleic acid.
abstract: An efficient chemoenzymatic method for preparing conjugated linoleic acid (CLA) using free linoleic acid (LA) as a substrate is described. In the first step, LA was transformed into 10-hydroxy-cis-12-octadecenoic acid (HA) by the whole cells of Lactobacillus plantarum after 48 h of incubation. The preincubation of whole cells with 0.03% LA resulted in a better yield of HA (480 mg/g) compared to cells grown without LA. In a second fast microwave step, HA was converted to cis-9,trans-11-octadecadienoic acid in the presence of iodine as a catalyst over a silica gel surface. The advantage of this method in preparing cis-9,trans-11 CLA is simple via the whole cell bioconversion of LA into HA via L. plantarum followed by the fast microwave-assisted synthesis of cis-9,trans-11 CLA in higher yields.
authors.affiliation: Department of Chemistry, Middle East Technical University, 06531Ankara, Turkey. asdemir@metu.edu.tr
authors.forename: Ayhan S
authors.initials: AS
authors.lastname: Demir
authors.forename: Farah N
authors.initials: FN
authors.lastname: Talpur
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000378:metabolism*; D019787:Linoleic Acid / Q000737:chemistry / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000378:metabolism*; D009829:Oleic Acids / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; C002446:12-octadecenoic acid; D019787:Linoleic Acid