title: Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region.
abstract: We present a three-color multiplex coherent anti-Stokes Raman scattering (CARS) setup that facilitates a prompt recording of broadband CARS spectra along with a fast CARS imaging. With separate narrowband Stokes and probe beams being introduced in the near IR, we are able to incorporate a stable, wideband Ti:sapphire femtosecond laser as a pump beam that covers the full range of Raman shift for CHn stretching vibrational modes. Experimentally, high-resolution multiplex CARS signals are allowed to investigate molecular vibrations over the range of 2650 cm-1 - 3050 cm-1, which are spectrally integrated to construct lipid-sensitive images. It is demonstrated that the proposed implementation promises a particular benefit on CARS imaging of lipid-rich tissue structures by providing detailed information on CHn Raman-active vibrations at points of interest on the CARS images that can be obtained at high frame rates.
authors.affiliation: Division of Convergence Technology, Korea Research Institute of Standards and Science, 1 Doryong-dong, Yuseong-gu, Daejeon 305-340, Korea. eslee@kriss.re.kr
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Se-Hwa
authors.initials: SH
authors.lastname: Kim
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Moon
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D002244:Carbon / Q000737:chemistry*; D003124:Colorimetry / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006859:Hydrogen / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D014732:Vibration
chemical_list: D002244:Carbon; D006859:Hydrogen