title: Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry.
abstract: Flow cytometry is an ever-advancing high-throughput multivariate analysis tool that natively provides size and morphological information. To obtain molecular information, however, typically requires the addition of fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. A complementary or alternative, label-free approach to molecular information is through multiplex coherent anti-Stokes Raman scattering (MCARS), which is a coherent, nonlinear optical method that provides a wealth of molecular information by probing the Raman energies within a molecule. In this work, we demonstrate the unique capability of our MCARS flow cytometer to distinguish flowing particles and discuss system performance capabilities and possibilities.
authors.affiliation: School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Dr., Atlanta, GA, 30332, USA. ccampjr@ece.gatech.edu
authors.forename: Charles H
authors.initials: CH
authors.lastname: Camp
authors.forename: Siva
authors.initials: S
authors.lastname: Yegnanarayanan
authors.forename: Ali A
authors.initials: AA
authors.lastname: Eftekhar
authors.forename: Hamsa
authors.initials: H
authors.lastname: Sridhar
authors.forename: Ali
authors.initials: A
authors.lastname: Adibi
mesh_terms: D001704:Biopolymers / Q000032:analysis*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013194:Staining and Labeling
chemical_list: D001704:Biopolymers