Chemically induced cardiomyogenesis of mouse embryonic stem cells.
A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of alpha-myosine heavy chain (alpha-MHC) promoter (palpha-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80 % increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets.
Berkessel
Albrecht
A
University of Cologne, Department of Chemistry, Greinstrasse 4, 50939 Cologne, Germany. berkessel@uni-koeln.de
Seelig
Bianca
B
Schwengberg
Silke
S
Hescheler
Jürgen
J
Sachinidis
Agapios
A
D000818:Animals; D002454:Cell Differentiation; D002930:Cinchona Alkaloids / Q000737:chemistry / Q000494:pharmacology; D015195:Drug Design; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D013890:Thiourea / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D002930:Cinchona Alkaloids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D013890:Thiourea