Reliable detection of dead microbial cells by using fluorescent hydrazides.
We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.
Saint-Ruf
Claude
C
INSERM U571, Faculté de Médecine Paris Descartes, Université René Descartes, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.
Cordier
Corinne
C
Mégret
Jérome
J
Matic
Ivan
I
D001419:Bacteria / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D050296:Microbial Viability; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes