A CD36 synthetic peptide inhibits silica-induced lung fibrosis in the mice.
Silicosis is a kind of pneumoconiosis caused by inhalation of silica dust, which is characterized by lung fibrosis. The biologically active form of transforming growth factor-beta1 (TGF-beta1) plays a key role in the development of lung fibrosis. CD36 is involved in the transformation of latent TGF-beta1 (L-TGF-beta1) to active TGF-beta1. The antagonistic effect of the synthetic peptide was analyzed by the administration of CD36 (93-110) synthetic peptide to the silicosis model of mice. The hydroxyproline content of the silica + CD36 (93-110) synthetic peptide group was significantly lower than that of the other experimental groups [silica and silica + CD36 (208-225) synthetic peptide groups] (p < .05). Inflammation, fibrotic degree and distribution of collagen fibers in silicotic nodules of the silica + CD36 (93-110) synthetic peptide group were less than those of the other experimental groups. The expressions of collagen I and III of the silica + CD36 (93-110) synthetic peptide group were significantly lower than those of the other experimental groups (p < .05). CD36 (93-110) synthetic peptide reduced the tissue fibrotic pathologies and collagen accumulation in the silicosis model of mice, resulting in the decreased severity of silica-induced lung fibrosis.
Wang
Xin
X
Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR China.
Lv
Lina
L
Chen
Ying
Y
Chen
Jie
J
D000818:Animals; D018955:CD36 Antigens / Q000627:therapeutic use*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis; D006909:Hydroxyproline / Q000378:metabolism; D019570:Inhalation Exposure; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008813:Mice, Inbred ICR; D010446:Peptide Fragments / Q000627:therapeutic use*; D010455:Peptides / Q000627:therapeutic use*; D012829:Silicosis / Q000188:drug therapy* / Q000378:metabolism
D018955:CD36 Antigens; C548810:CD36 protein (93-110); D024042:Collagen Type I; D024061:Collagen Type III; D010446:Peptide Fragments; D010455:Peptides; D006909:Hydroxyproline