In vitro drug interactions of gallates with antibiotics in Staphylococcus Aureus.
The antimicrobial activities of tetracycline, mupirocin, and fusidic acid are tested in combination with Epicatechin Gallate (ECG), and Ethyl Gallate (EG) using 2 Methicillin resistant (MRSA) and 2 Methicillin sensitive (MSSA) strains of Staphylococcus aureus. Sub-inhibitory concentration of EG at 256 mg l-1 is found to be synergistic when used in combination with tetracycline, mupirocin, and fusidic acid; and a sub-inhibitory concentration of ECG at 32 mg l-1 is found to be synergistic with tetracycline in all the four Staphylococcus aureus strains tested. The synergistic combinations reduce the MICs of all the above three antibiotics by 4 fold. Combining ECG at 32 mg l-1 with mupirocin, reduces the MIC of mupirocin by four fold in MSSA C1 strain. 74 per cent of the combinations show consistent results in both time-kill assay and checkerboard method. The identified combinations may lead towards novel therapeutic interventions for treating MRSA infections.
Soe
Win Mar
WM
Advanced Design and Modeling Lab, Nanyang Technological University, Singapore.
Tzer Pin Lin
Raymond
R
Chu Sing Lim
Lim
L
Sakharkar
Kishore R
KR
Sakharkar
Meena K
MK
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004347:Drug Interactions / Q000502:physiology*; D005672:Fusidic Acid; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016712:Mupirocin; D013752:Tetracycline
D000900:Anti-Bacterial Agents; C048734:ethyl gallate; D005672:Fusidic Acid; D005707:Gallic Acid; D002392:Catechin; C062669:epicatechin gallate; D016712:Mupirocin; D013752:Tetracycline