title: Effect of Wujijing Oral Liquid on menstrual disturbance of women.
abstract: OBJECTIVE
  Wujijing Oral Liquid (WJJ) contained principally the flesh essence of the black-boned chicken. As a kind of food and medicine in China, it was used to treat the menstrual disturbance traditionally, but the exact mechanism of the action was not yet clear. The clinical effects of the WJJ on the symptoms of the menstrual disturbance and the reproductive hormones were studied in this paper.
  
  
  METHODS
  The 53 women with the menstrual disturbance were selected as the study object, and then they were randomly divided to receive either WJJ 10mL twice daily (n=28) or the placebo (n=25) from the 1st day after menstrual flow for 2 menstrual cycles. On the 1st day after the discontinuation of the medication but before the treatment, the scores for the menstrual pattern and the related symptoms were obtained and the blood samples were collected to test the reproductive hormones. The serum levels of the follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and estradiol (E2) were examined by enzyme-linked immunosorbent assay (ELISA). The levels of progesterone (P) and testosterone (T) in serum were measured by the radioimmunoassay.
  
  
  RESULTS
  The score for the primary and related symptoms of the menstruation was increased significantly among patients treated with the WJJ. The differences on the FSH, PRL, and E2 levels of patients were significant before and after the treatment with WJJ. Comparing the WJJ group and the placebo group, the levels of P and T differed significantly after treatment. The oral liquid of WJJ was found to be safe, as it did not cause any change in the hepatic and renal functional parameters.
  
  
  CONCLUSIONS
  The oral liquid of Wujijing could improve the menstrual disturbance and were generally safe and well tolerated. The possible mechanism could be associated with its effects in reinforcing the kidney and regulating the hypothalamus-pituitary-ovary axis (HPOA).
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, 140 Han Zhong Road, Nanjing 210029, China.
authors.forename: Shan Shan
authors.initials: SS
authors.lastname: Geng
authors.forename: Hai Zhong
authors.initials: HZ
authors.lastname: Li
authors.forename: Xiao Ke
authors.initials: XK
authors.lastname: Wu
authors.forename: Jie Ming
authors.initials: JM
authors.lastname: Dang
authors.forename: Hua
authors.initials: H
authors.lastname: Tong
authors.forename: Cui Ying
authors.initials: CY
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yun Qing
authors.initials: YQ
authors.lastname: Cai
mesh_terms: D000328:Adult; D002681:China; D004304:Dosage Forms; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000494:pharmacology; D008516:Medicine, Chinese Traditional; D008597:Menstrual Cycle / Q000187:drug effects / Q000502:physiology; D008598:Menstruation / Q000097:blood; D008599:Menstruation Disturbances / Q000097:blood / Q000209:etiology*; D011374:Progesterone / Q000097:blood / Q000494:pharmacology; D011388:Prolactin / Q000097:blood / Q000494:pharmacology; D011863:Radioimmunoassay; D013739:Testosterone / Q000097:blood / Q000494:pharmacology
chemical_list: D004304:Dosage Forms; D006728:Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone