title: Triazole-containing BODIPY dyes as novel fluorescent probes for soluble oligomers of amyloid Abeta1-42 peptide.
abstract: A straightforward functionalization of BODIPY dyes via incorporation of a triazole moiety produced fluorescent dyes that were capable of distinguishing between secondary structure conformations of soluble oligomeric species of Abeta1-42 peptide. Small concentrations of the dyes, relative to Abeta1-42, provided up to an 8-fold and 35-fold fluorescence increase in the presence of the unordered and ordered, beta-sheet-rich conformations of soluble Abeta1-42 oligomers, respectively. These triazole-containing dyes could prove to be useful probes for monitoring amyloid conformational transitions in vitro.
authors.affiliation: Department of Chemistry, Texas Christian University, Fort Worth, TX 76129, USA.
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Smith
authors.forename: Annabel
authors.initials: A
authors.lastname: Alonso
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Brown
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Dzyuba
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D014230:Triazoles / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D016229:Amyloid beta-Peptides; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D014230:Triazoles; C075222:amyloid beta-protein (1-42)