[A novel formulation of cisplatin (CDDP)].
The study aimed to assess our newly devised CDDP delivery system designed to provide targeting potential and a sustained release of the anticancer agent. Seventy percent deacetylated chitin was applied as a drug carrier. We prepared two different types of systems with two different procedures: namely, system A with direct method and system B with indirect method. The targeting property of the system was evaluated ex vivo with measuring adhesive force between each system and human colonic mucosa. The release behavior of the CDDP from the system was examined in vitro. The anticancer activities of the released CDDP were also examined in vitro using human gastric cancer cell line, MKN-45. Each system was a viscose elastic solution. The adhesive forces of the novel systems were stronger at 37 degrees C than that of 25 degrees C. Each system provided a sustained release of CDDP. The released CDDP demonstrated effective growth suppression activity against the MKN-45 cancer cells. The novel systems basically showed a favorable targeting function and a sustained release of CDDP, which effectively provided a growth inhibition potential against human cancer cell line. Our newly devised CDDP delivery systems are promising as a novel approach to cancer chemotherapy.
Sugitachi
Akio
A
Department of Surgery, Iwate Medical University.
Kimura
Yusuke
Y
Itabashi
Tetsuya
T
Noda
Hironobu
H
Ishida
Kazushige
K
Nishizuka
Satoshi
S
Otsuka
Koki
K
Kashiwaba
Masahiro
M
Nitta
Hiroyuki
H
Koeda
Keisuke
K
Mizuno
Masaru
M
Sasaki
Akira
A
Ikeda
Kenichiro
K
Takamori
Yoshimori
Y
Wakabayashi
Go
G
D000268:Adhesiveness; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002686:Chitin; D002945:Cisplatin / Q000008:administration & dosage* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D002686:Chitin; D002945:Cisplatin