Effect of different microcystin profiles on toxin bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii.
In this study, a 12-day growth trial was conducted to compare the effect of the variation in microcystin (MC) composition in two Microcystis aeruginosa bloom samples on the growth performance and MC accumulation/transfer in the common carp (Cyprinus carpio L.) larvae. Fish were fed Artemia salina nauplii that had been preexposed to extracts from two M. aeruginosa natural blooms with different microcystins (MCs) profiles. Bloom A had MC-LR as major toxin (74.05%) while bloom B had a diversity of MC (MC-RR; MC-(H4)YR; MC-YR; MC-LR; MC-FR; MC-WR) with no dominance of MC-LR. Newly-hatched Artemia nauplii were exposed separately to the two M. aeruginosa extracts A and B (100 microg L(-1)EqMC-LR) for 2h. The MC concentration in the nauplii was 73.60+/-7.88ngEqMC-LRg(-1)FW (n=4, mean+/-SE) for bloom A and 87.04+/-10.31ngEqMC-LRg(-1)FW for bloom B. These contaminated nauplii were given at the same ration to different groups (A and B) of fish larvae. Larval weight and length from day 9 were significantly different between groups A and B, and in both cases lower than that of a control group fed non-exposed nauplii. MCs accumulation by larvae, inversely correlated with the growth performance, was also significantly different between groups A and B (37.43+/-2.61 and 54.55+/-3.01ngEqMC-LRg(-1) FW, respectively, at the end of the experimental period). These results indicate that MC profile of a bloom may have differential effects on toxin accumulation/transfer and toxicity.
El Ghazali
Issam
I
Department of Biology, Laboratory of Biology and Biotechnology of Microorganisms, Microbiology and Environmental Toxicology Unit, Faculty of Sciences Semlalia Marrakech, University Cadi Ayyad, PO Box 2390, Marrakech 40000, Morocco.
Saqrane
Sanaa
S
Carvalho
António Paulo
AP
Ouahid
Youness
Y
Del Campo
Francisca F
FF
Oudra
Brahim
B
Vasconcelos
Vitor
V
D000818:Animals; D001156:Artemia / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D020387:Food Chain; D007814:Larva / Q000378:metabolism; D052998:Microcystins / Q000378:metabolism / Q000633:toxicity*; D046931:Microcystis / Q000254:growth & development / Q000378:metabolism
D002273:Carcinogens; D052998:Microcystins; C078588:microcystin