title: Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii.
abstract: In bacteria and plants, dihydrodipicolinate synthase (DHDPS) plays a key role in the (S)-lysine biosynthesis pathway. DHDPS catalyzes the first step of the condensation of (S)-aspartate-beta-semialdehyde and pyruvate to form an unstable compound, (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid. The activity of DHDPS is allosterically regulated by (S)-lysine, a feedback inhibitor. The crystal structure of DHDPS from Methanocaldococcus jannaschii (MjDHDPS) was solved by the molecular-replacement method and was refined to 2.2 A resolution. The structure revealed that MjDHDPS forms a functional homotetramer, as also observed in Escherichia coli DHDPS, Thermotoga maritima DHDPS and Bacillus anthracis DHDPS. The binding-site region of MjDHDPS is essentially similar to those found in other known DHDPS structures.
authors.affiliation: Systems and Structural Biology Center, Yokohama Institute, RIKEN, 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan. bpadmanabhan@hotmail.com
authors.forename: Balasundaram
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: Richard W
authors.initials: RW
authors.lastname: Strange
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Antonyuk
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Ellis
authors.forename: S Samar
authors.initials: SS
authors.lastname: Hasnain
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iino
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Agari
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Bessho
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000201:enzymology / Q000235:genetics; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D019766:Genes, Archaeal; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics; D017015:Methanococcales / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein
chemical_list: D011994:Recombinant Proteins; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase