Development and validation of a rapid method for the detection of latrunculol A in plasma.
Latrunculol A is a recently discovered 6,7-dihydroxy analog of the potent actin inhibitor latrunculin A. Latrunculol A has exhibited greater cytotoxicity than latrunculin A against both murine and human colon tumor cell lines in vitro. Currently, there are no reports regarding the bioavailability of latrunculol A in vivo. This study was undertaken as a prelude to pharmacokinetic assessments and it is the first work where bioavailability of latrunculol A was studied. In the present work, a simple plasma preparation and a rapid HPLC method have been developed. Mouse plasma containing latrunculol A was first treated by acetonitrile and then centrifuged at 14,000 rpm at 4 °C for 25 min. The supernatant was injected in an HPLC system comprising a Waters Symmetry NH(2) column, a mobile phase of acetonitrile/water (95/5, v/v), a flow rate of 1.0 mL/min, at 220 nm. The method was validated by parameters including a good linear correlation, a limit of quantification of 9 ng/mL, and a good precision with a coefficient variation of 1.65, 1.86, and 1.26% for 20, 400, and 800 ng/mL, respectively. With this simple method, excellent separation and sensitivity of latrunculol A are achieved, thus allowing a rapid analysis of the plasma samples for absorption, distribution, and metabolism studies.
Shaw
Jiajiu
J
Henry Ford Health System, Detroit, MI 48202, USA. jiajiushaw@gmail.com
Valeriote
Frederick A
FA
Media
Joseph
J
Johnson
Tyler A
TA
Amagata
Taro
T
Tenney
Karen
K
Crews
Phillip
P
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D018942:Macrolides / Q000097:blood* / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D053778:Thiazolidines / Q000097:blood* / Q000737:chemistry; D013997:Time Factors
D018942:Macrolides; D053778:Thiazolidines; C555732:latrunculol A