title: Spatio-temporal PLC activation in parallel with intracellular Ca2+ wave propagation in mechanically stimulated single MDCK cells.
abstract: Intracellular Ca2+ transients are evoked either by the opening of Ca2+ channels on the plasma membrane or by phospholipase C (PLC) activation resulting in IP3 production. Ca2+ wave propagation is known to occur in mechanically stimulated cells; however, it remains uncertain whether and how PLC activation is involved in intracellular Ca2+ wave propagation in mechanically stimulated cells. To answer these questions, it is indispensable to clarify the spatio-temporal relations between intracellular Ca2+ wave propagation and PLC activation. Thus, we visualized both cytosolic Ca2+ and PLC activation using a real-time dual-imaging system in individual Mardin-Darby Canine Kidney (MDCK) cells. This system allowed us to simultaneously observe intracellular Ca2+ wave propagation and PLC activation in a spatio-temporal manner in a single mechanically stimulated MDCK cell. The results showed that PLC was activated not only in the mechanically stimulated region but also in other subcellular regions in parallel with intracellular Ca2+ wave propagation. These results support a model in which PLC is involved in Ca2+ signaling amplification in mechanically stimulated cells.
authors.affiliation: Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tsukamoto
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Hayashida
authors.forename: Katsuko S
authors.initials: KS
authors.lastname: Furukawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D004789:Enzyme Activation / Q000502:physiology; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007295:Inositol Phosphates / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D010812:Physical Stimulation; D015398:Signal Transduction / Q000502:physiology*; D013314:Stress, Mechanical; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D010738:Type C Phospholipases; D002118:Calcium