title: Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography.
abstract: Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.
authors.affiliation: Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ju
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Cao
authors.forename: Jihong
authors.initials: J
authors.lastname: Jiang
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito