Antibacterial activity of N-pentylpantothenamide is due to inhibition of coenzyme a synthesis.
Growth inhibition by the pantothenate analog N-pentylpantothenamide (N5-Pan) has been attributed to the accumulation of acyl carrier protein carrying a prosthetic group modified by incorporation of N5-Pan. This was attributed to an inability of the AcpH acyl carrier protein phosphodiesterase to cleave the N5-Pan-modified prosthetic group from the protein moiety. We report that AcpH readily removes the N5-Pan-modified prosthetic group both in vivo and in vitro and show that N5-Pan blocks coenzyme A synthesis.
Thomas
Jacob
J
Departments of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Cronan
John E
JE
D000213:Acyl Carrier Protein / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003065:Coenzyme A / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism / Q000494:pharmacology
D000213:Acyl Carrier Protein; D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C470569:N-pentylpantothenamide; D010205:Pantothenic Acid; D010727:Phosphoric Diester Hydrolases; C018754:(acyl-carrier-protein) phosphodiesterase; D003065:Coenzyme A