title: Enantioseparation of alpha-amino acids on an 18-crown-6-tetracarboxylic acid-bonded silica by capillary electrochromatography.
abstract: (-)-(18-Crown-6)-2,3,11,12-tetracarboxylic acid-bonded silica was used as the chiral stationary phase in capillary electrochromatography (CEC) for enantioseparation of some alpha-amino acids. Separation data in CEC were measured in mobile phases of varying pH, and composition of methanol and buffer, and compared with those in capillary liquid chromatography (CLC). In CEC better enantioseparation was generally obtained in the eluent of lower pH, higher buffer concentration and intermediate MeOH content, usually at the expense of analysis time. CEC showed generally better enantioselectivity and resolutions than CLC for the amino acids investigated.
authors.affiliation: Department of Chemistry, Yeungnam University, 214-1 Daedong, Gyeongsan 712-749, South Korea.
authors.forename: Taeho
authors.initials: T
authors.lastname: Lee
authors.forename: Wonjae
authors.initials: W
authors.lastname: Lee
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Hyun
authors.forename: Jung Hag
authors.initials: JH
authors.lastname: Park
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000191:economics / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D043844:Crown Ethers; C090732:18-crown-6 2,3,11,12-tetracarboxylic acid; D012822:Silicon Dioxide; D000432:Methanol