title: Development of microfluidic-based heterogeneous immunoassays.
abstract: Microfluidic-based heterogeneous immunoassays are reviewed in this article by first introducing the principle of immunoassay, followed by a discussion of microfluidic-based technology. Microfabrication, surface modification, solution dispensing and detection technology are discussed and their applications to biomolecular detection reviewed. In the future, improved manufacturing processes and integrated assay systems in an automatic fashion with a reduced assay time and reagent consumption will allow for the effective detection of biomolecules that are of interest in medical diagnostics, drug discovery and bioterrorism.
authors.affiliation: Southlake Regional Health Centre, Newmarket, Ontario, Canada. franklin088@yahoo.ca
authors.forename: Frank Yung Harn
authors.initials: FY
authors.lastname: Lin
authors.forename: Yali
authors.initials: Y
authors.lastname: Gao
authors.forename: Dongqing
authors.initials: D
authors.lastname: Li
authors.forename: Philip Martin
authors.initials: PM
authors.lastname: Sherman
mesh_terms: D004563:Electrochemistry / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods