AQP1 mediates water transport in the carotid body.
In this study, we explored the presence of aquaporins (AQPs), a family of membrane water channel proteins, in carotid body (CB) type I chemoreceptor cells. The CB is a polymodal chemoreceptor whose major function is to detect changes in arterial O2 tension to elicit hyperventilation during hypoxia. The CB has also been proposed to function as a systemic osmoreceptor, thus we hypothesized that the presence of AQPs in type I cell membrane may confer higher sensitivity to osmolarity changes and hence accelerate the activation of chemoreceptor cells. We detected the expression of AQP1, AQP7, and AQP8 in the CB and confirmed the location of AQP1 in type I cells. We have also shown that inhibition of AQP1 expression clearly reduced type I cell swelling after a hyposmotic shock, demonstrating that AQP1 has a major contribution in transmembrane water movement in these chemoreceptor cells. Interestingly, CB AQP1 expression levels change during postnatal development, increasing during the first postnatal weeks as the organ matures. In conclusion, in this study, we report the novel observation that AQPs are expressed in the CB. We also show that AQP1 mediates water transport across the cell membrane of type I cells, supporting the contribution of this protein to the osmoreception function of the CB.
Muñoz-Cabello
Ana M
AM
Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville 41013, Spain. ana.munoz@cabimer.es
Villadiego
Javier
J
Toledo-Aral
Juan J
JJ
López-Barneo
José
J
Echevarría
Miriam
M
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology*; D002344:Carotid Body / Q000502:physiology*; D005786:Gene Expression Regulation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014867:Water / Q000378:metabolism*
C496022:Aqp1 protein, rat; D012333:RNA, Messenger; D014867:Water; D051398:Aquaporin 1