[Diversity and bioactivities of cultured actinomycete isolated from animal feces and Xylocopa dissimilis].
OBJECTIVE
To study the diversity and bioactivities of cultured actinomycete from feces of Ailuropoda melanoleuca, Equus burchelli and Rhizomys sinensis, and Xylocopa dissimilis body.


METHODS
Fresh feces samples of animals' and Xylocopa dissimilis were collected. We used four media to isolate the actinomycete. Strains were identified by phylogenetic analysis of 16S rRNA gene sequences. Hydrolization and enzyme activities of isolates were examined.


RESULTS
Actinomycetes (121) from the samples were isolated on four media. Among them 47 strains belonged to 9 actinomycetes genera. Six known rare genera and one possible new genus of actinomyctes from Rhizomys sinensis were identified. Three possible new species from other three samples were identified. Some strains could hydrolyze chicken feather and cellulose.


CONCLUSIONS
It is considered that the coprophilous microorganisms not only have important function on the digestion and absorption of animal feed, but also are important resource for development of industrial products, including enzyme and bioactive metabolites.
Jiang
Yi
Y
Yunnan Institute of Microbiology, The National Engineering Center for Research of Microbial Pharmaceuticals, Yunnan University, Kunming 650091, China. jiangyikm@hotmail.com
Cao
Yanru
Y
Cai
Xiangfeng
X
Xu
Lihua
L
Jiang
Chenglin
C
D039903:Actinobacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D046508:Culture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S