title: Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium.
abstract: BACKGROUND
  To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube.
  
  
  METHODS
  The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted using a total RNA isolation kit. Semi-quantitative RT-PCR was performed to examine mRNA relative expression.
  
  
  RESULTS
  Plexin-B1 expression in the endometrium was significantly higher on days 19 - 23 compared to days 12 - 14 (1.166 +/- 0.42 versus 0.523 +/- 0.299), P < 0.005. In the fallopian tube the level of plexin-B1 did not change significantly throughout the menstrual cycle. Glycodelin expression was significantly higher on days 19 - 23 compared with days 12-14, both in the endometrium (0.819 +/- 0.564 versus 0.072 +/- 0.343, P < 0.05) and the fallopian tube (0.796 +/- 0.196 versus 0.329 +/- 0.398, P < 0.05). Although the level of MMP7 secretion was the highest in the secretory phase the difference from the proliferative phase did not reach statistical significance, neither in the endometrium nor in the fallopian tube. This could result from a lack of power.
  
  
  CONCLUSIONS
  In the endometrium, both Glycodelin and Plexin-B1 are exhibiting a cyclic pattern suggesting a possible steroid regulation and a role in endometrial receptivity.
authors.affiliation: Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, 18101, Afula, Israel. nachshon4@gmail.com
authors.forename: Michal
authors.initials: M
authors.lastname: Amir
authors.forename: Shabtai
authors.initials: S
authors.lastname: Romano
authors.forename: Shlomit
authors.initials: S
authors.lastname: Goldman
authors.forename: Eliezer
authors.initials: E
authors.lastname: Shalev
mesh_terms: D000328:Adult; D017931:DNA Primers; D004717:Endometrium / Q000201:enzymology / Q000378:metabolism*; D005187:Fallopian Tubes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D000071816:Glycodelin; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis* / Q000235:genetics*; D008598:Menstruation / Q000502:physiology; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D000071816:Glycodelin; D006023:Glycoproteins; D009419:Nerve Tissue Proteins; C045246:PAEP protein, human; C492119:PLXNB1 protein, human; D011257:Pregnancy Proteins; D011956:Receptors, Cell Surface; D012313:RNA; D020783:Matrix Metalloproteinase 7