title: [Aporphine alkaloids from branches and leaves of Polyalthia nemoralis].
abstract: OBJECTIVE
  To investigate the aporphine alkaloids in the branches and leaves of Polyalthia nemoralis.
  
  
  METHODS
  The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.
  
  
  RESULTS
  Five aporphine alkaloids were isolated and identified as: bidebiline A (1), annobraine (2), lanuginosine (3), liriodenine (4), oxostephanosine (5), respectively.
  
  
  CONCLUSIONS
  For the first time, Compounds 2 and 5 were obtained from Polyalthia while 1, 3 and 4 isolated from this plant. The bioassays in vitro against five human tumor cell lines with MTT method showed moderate cytotoxic activities (IC50 1 mg x L(-1)) of compounds 3-5.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Lu
authors.forename: Qingjian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ruoyun
authors.initials: R
authors.lastname: Chen
authors.forename: Dequan
authors.initials: D
authors.lastname: Yu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001060:Aporphines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D031044:Polyalthia / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001060:Aporphines