High-concentration sugars production from corn stover based on combined pretreatments and fed-batch process.
In this paper, high-concentration sugars were produced from pretreated corn stover. The raw corn stover was pretreated in a process combining steam explosion and alkaline hydrogen-peroxide. The hemicellulose and lignin were removed greatly. The cellulose content increased to 73.2%. Fed-batch enzymatic hydrolysis was initiated with 12% (w/v) solids loading and 20 FPU/g solids. Then, 6% solids were fed consecutively at 12, 36 and 60 h. After 144 h, the final concentrations of reducing sugar, glucose, cellobiose and xylose reached 220, 175, 22 and 20 g/L, respectively. The final total biomass conversion was 60% in fed-batch process.
Yang
Maohua
M
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Li
Wangliang
W
Liu
Binbin
B
Li
Qiang
Q
Xing
Jianmin
J
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry*; D002475:Cellobiose / Q000737:chemistry; D004736:Energy-Generating Resources; D004798:Enzymes / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013997:Time Factors; D003313:Zea mays
D002241:Carbohydrates; D004798:Enzymes; D011134:Polysaccharides; D002475:Cellobiose; C007916:hemicellulose; D008031:Lignin; D006861:Hydrogen Peroxide; D005947:Glucose