Mechanism of inactivation of murine norovirus-1 by high pressure processing.
Murine norovirus-1 (MNV) is currently the most suitable surrogate for human norovirus. The mechanism of MNV-1 inactivation by high pressure processing (HPP) was investigated. HPP-treated MNV could not bind to its target receptor and therefore could not initiate infection of mouse RAW cells. The integrity of the capsid was not affect by HPP. Partial motif changes of the viral capsid caused by HPP were accessed by induced sensitivity to proteinase K.
Tang
Qingjuan
Q
College of Food Science and Engineering, Ocean University of China, No 5, Yushan Road, Qingdao, Shandong Province 266003, PR China.
Li
Dan
D
Xu
Jie
J
Wang
Jingfeng
J
Zhao
Yuran
Y
Li
Zhaojie
Z
Xue
Changhu
C
D000818:Animals; D002460:Cell Line; D019286:Endopeptidase K / Q000378:metabolism; D006874:Hydrostatic Pressure; D008264:Macrophages / Q000821:virology; D051379:Mice; D050296:Microbial Viability; D029322:Norovirus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism; D038301:Virus Inactivation
D014764:Viral Proteins; D019286:Endopeptidase K