title: Structure of a putative beta-phosphoglucomutase (TM1254) from Thermotoga maritima.
abstract: The structure of TM1254, a putative beta-phosphoglucomutase from T. maritima, was determined to 1.74 A resolution in a high-throughput structural genomics programme. Diffraction data were obtained from crystals belonging to space group P22(1)2(1), with unit-cell parameters a = 48.16, b = 66.70, c = 83.80 A, and were refined to an R factor of 19.2%. The asymmetric unit contained one protein molecule which is comprised of two domains. Structural homologues were found from protein databases that confirmed a strong resemblance between TM1254 and members of the haloacid dehalogenase (HAD) hydrolase family.
authors.affiliation: Molecular Biophysics Group, School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, England. r.strange@liverpool.ac.uk
authors.forename: Richard W
authors.initials: RW
authors.lastname: Strange
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Antonyuk
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Ellis
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Bessho
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Akeo
authors.initials: A
authors.lastname: Shinkai
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: S Samar
authors.initials: SS
authors.lastname: Hasnain
mesh_terms: D020134:Catalytic Domain; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D005798:Genes, Bacterial; D008958:Models, Molecular; D010733:Phosphoglucomutase / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D040681:Structural Homology, Protein; D020124:Thermotoga maritima / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D010733:Phosphoglucomutase