Application of immunoassay for detection of Helicobacter pylori antigens in the dental plaque.
OBJECTIVE
The aim of this study was to determine the viability of the commercial test currently used for detection of H. pylori antigens in the stool for detection of H. pylori antigens in dental plaque.


METHODS
A total of 164 dyspeptic patients entered the study; 95 H. pylori infected (positive result of at least 4 of 5 diagnostic tests: Campylobacter-like organisms test (CLO test), histology, culture, stool antigens, serology) and 69 noninfected (negative results of 4 diagnostic tests: CLO test, histology, culture, stool antigens). Dental plaque was collected from natural teeth of the patients and incubated in microaerophilic conditions for 72 hours before immunoassay.


RESULTS
Experimental findings included that optimal dental plaque weight to perform the examination was over 2 mg and that preliminary incubation increased significantly the number of positive results (p<0.002). It was also found that H. pylori antigens in the dental plaque were positive in 81.2% of infected and only 17.7% of non-infected subjects (p<0.001), while the reproducibility of results was 95%.


CONCLUSIONS
The immunoassay for detection of H. pylori antigens in the stool may be used, after minor adaptations (specifically pre-incubation in microaerophilic conditions) for H. pylori antigen detection in dental plaque.
Leszczyńska
K
K
Department of Diagnostic Microbiology, Medical University of Białystok, Białystok, Poland.
Namiot
D B
DB
Namiot
Z
Z
Leszczyńska
J K
JK
Jakoniuk
P
P
Kemona
A
A
D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis* / Q000097:blood; D001431:Bacteriological Techniques; D001706:Biopsy; D002167:Campylobacter / Q000276:immunology; D003773:Dental Plaque / Q000276:immunology* / Q000382:microbiology; D004415:Dyspepsia / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D055815:Young Adult
D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G