Simultaneous optical and electrical single channel recordings on a PEG glass.
Single molecule imaging of working ion-channels is much more difficult than that of water-soluble proteins because of the fragile nature of membranes and lateral diffusion of particles in the membranes, which does not allow fluorescent contamination for optical single channel recording. In this report, we reconstituted maxi-potassium channels from porcine uterine smooth muscle into artificial planar bilayers formed on poly(ethylene glycol) (PEG) modified glass and performed simultaneous optical and electrical recording of the single channels. The channels were immobilized in the membranes by anchoring to PEG molecules on the glass. The technique developed in this study should pave the way for single molecule pharmacology of ion-channels.
Ide
Toru
T
Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan. ide@phys1.med.osaka-u.ac.jp
Takeuchi
Yuko
Y
Noji
Hiroyuki
H
Tabata
Kazuhito V
KV
D000818:Animals; D002417:Cattle; D005260:Female; D005898:Glass; D007473:Ion Channels / Q000502:physiology*; D011092:Polyethylene Glycols; D013552:Swine
D007473:Ion Channels; D011092:Polyethylene Glycols