[Transgenesis and humanization of murine antibodies].
The properties of monoclonal antibodies explain why they are such a successful class of therapeutic molecules. However, pionneered initial antibodies were of murine origin and triggered an immune response which limited the therapeutic potential of the antibody and generated deleterious effects. Consequently, tremendous efforts have been developped to engineer these murine Ig by introducing human sequences in vitro, or in vivo by humanization of murine antibodies, leading to chimeric immunoglobulins, and more recently generation of fully human antibodies in transgenic mice with a more or less diversified V repertoire. These approaches have led to the development of an increasing number of these chimeric or humanized monoclonal antibodies entering pharmaceutical pipelines.
Cogné
Michel
M
Université de Limoges, Laboratoire d'immunologie, Faculté de médecine, F-87025 Limoges, France. michel.cogne@unilim.fr
Duchez
Sophie
S
Pascal
Virginie
V
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000096:biosynthesis; D000916:Antibody Diversity; D005544:Forecasting; D005803:Genes, Immunoglobulin; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D013045:Species Specificity; D019076:Transgenes
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins