title: Early removal of alternatively activated macrophages leads to Taenia crassiceps cysticercosis clearance in vivo.
abstract: To determine the role of alternatively activated macrophages in modulating the outcome of experimental cysticercosis caused by Taenia crassiceps, we investigated the effect of removal of alternatively activated macrophage by injecting clodronate-loaded liposomes into susceptible BALB/c mice. Following T. crassiceps infection, mice receiving PBS-loaded liposomes developed a dominant Th2-type response associated with the presence of alternatively activated macrophages together with antigen-specific hyporesponsiveness and high parasite burden. In contrast, similarly infected mice treated with clodronate-loaded liposomes mounted a mixed Th1/Th2-type response, reversed antigen-specific hyporesponsiveness and did not carry notable alternatively activated macrophage populations. These factors were associated with increased resistance to T. crassiceps cysticercosis. Interestingly, early AAM phi depletion was enough to limit parasite growth. However, if macrophages were depleted late in the infection, no effect on parasite burden was observed. These findings demonstrate that alternatively activated macrophages play a critical role in mediating susceptibility to experimental cysticercosis in which their early recruitment may favor parasite survival.
authors.affiliation: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Mexico.
authors.forename: José L
authors.initials: JL
authors.lastname: Reyes
authors.forename: César A
authors.initials: CA
authors.lastname: Terrazas
authors.forename: Javier
authors.initials: J
authors.lastname: Alonso-Trujillo
authors.forename: Nico
authors.initials: N
authors.lastname: van Rooijen
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Satoskar
authors.forename: Luis I
authors.initials: LI
authors.lastname: Terrazas
mesh_terms: D000818:Animals; D003551:Cysticercosis / Q000276:immunology* / Q000469:parasitology*; D005260:Female; D047589:Leukocyte Reduction Procedures; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013621:Taenia / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology