title: Micronucleus frequency in buccal mucosa cells of mobile phone users.
abstract: Mobile phones are being used extensively throughout the world, with more than four billion accounts existing in 2009. This technology applies electromagnetic radiation in the microwave range. Health effects of this radiation have been subject of debate for a long time, both within the scientific community and within the general public. This study investigated the effect of mobile phone use on genomic instability of the human oral cavity's mucosa cells. 131 Individuals donated buccal mucosa cells extracted by slightly scraping the oral cavity with a cotton swab. Every participant filled out a questionnaire about mobile phone use including duration of weekly use, overall period of exposure and headset usage. 13 Individuals did not use mobile phones at all, 85 reported using the mobile phone for three hours per week or less, and 33 reported use of more than three hours per week. Additionally, information on age, gender, body weight, smoking status, medication and nutrition was retrieved. For staining of the cells a procedure using alpha-tubulin-antibody and chromomycin A(3) was applied. Micronuclei and other markers were evaluated in 1000 cells per individual at the microscope. A second scorer counted another 1000 cells, resulting in 2000 analyzed cells per individual. Mobile phone use did not lead to a significantly increased frequency of micronuclei.
authors.affiliation: Department of Toxicology, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Hintzsche
authors.forename: Helga
authors.initials: H
authors.lastname: Stopper
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D040421:Cell Phone; D015331:Cohort Studies; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D011829:Radiation Dosage; D012727:Sex Characteristics; D055815:Young Adult
chemical_list: D004247:DNA