Prevention of primary vascular graft infection with silver-coated polyester graft in a porcine model.
OBJECTIVE
To evaluate the efficacy of a silver-coated vascular polyester graft in the prevention of graft infection after inoculation with Staphylococcus aureus in a porcine model.


METHODS
Eighty-four pigs were randomly selected 1:1 to receive a silver-coated or non-silver-coated 8-mm-wide polyester graft implanted end-to-end in the infrarenal aorta. At the end of implantation, 10(6) colony forming units (CFUs) S. aureus in 0.3ml suspension were inoculated directly on the graft surface. Blood samples assayed for white blood corpuscles (WBCs) and C-reactive protein (CRP) were taken before implantation and on the postoperative days 2, 5, 7, 11 and 14. Two weeks after implantation, the perigraft swabs were analysed for S. aureus or contaminants. CFUs of S. aureus were quantified and logarithmised. Student's t-tests, repeated measurement analysis of variance (ANOVA) and chi-square test were employed to compare the two grafts.


RESULTS
All pigs developed graft infection. There were no statistically significant differences between the silver-coated and non-silver-coated grafts in the quantity of S. aureus, macroscopic signs of infection and postoperative changes in the temperature, WBC and CRP.


CONCLUSIONS
Silver-coated polyester grafts failed to prevent graft infections after inoculation with 10(6)S. aureus in 0.3ml suspension in a porcine model.
Gao
H
H
Vascular Research Unit, Department of Vascular Surgery, Regional Hospital Viborg, Viborg, Denmark. gao.hong@viborg.rm.dk
Sandermann
J
J
Prag
J
J
Lund
L
L
Lindholt
J S
JS
D000085:Acetates / Q000008:administration & dosage*; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001011:Aorta / Q000382:microbiology / Q000601:surgery; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D001831:Body Temperature; D002097:C-Reactive Protein / Q000378:metabolism; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D005260:Female; D007958:Leukocyte Count; D008422:Materials Testing; D011091:Polyesters; D016459:Prosthesis-Related Infections / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*; D018030:Silver Compounds / Q000008:administration & dosage*; D013203:Staphylococcal Infections / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000472:pathogenicity; D013552:Swine; D013997:Time Factors; D017211:Treatment Failure
D000085:Acetates; D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D018030:Silver Compounds; C103100:silver acetate; D002097:C-Reactive Protein