[Absorption characteristics of ginsenoside Rb2 in Caco-2 cell monolayer].
OBJECTIVE
To determine the concentration of ginsenoside Rb(2) and study the absorption characteristics of ginsenoside Rb(2) in Caco-2 cell monolayer.


METHODS
LC-MS-MS was used to determine the concentration of ginsenoside Rb(2), and the apparent permeability coefficient (P(app)) of ginsenoside Rb(2) was calculated.


RESULTS
P(app(AP-BL)) was 3.27 x 10(-7) cm.s(-1), P(app(BL-AP)) was 3.16 x 10(-6) cm.s(-1), and the efflux ratio (P(app(BL-AP))/P(app(AP-BL))) was 9.63.


CONCLUSIONS
The absorption characteristics of ginsenoside Rb(2) in Caco-2 cell model have been demonstrated.
Zhao
Jie
J
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. zhaojie_0412@163.com
Yang
Cai-hua
CH
Hu
Ming
M
Liu
Zhong-qiu
ZQ
D018938:Caco-2 Cells; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D036145:Ginsenosides / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption
D036145:Ginsenosides; C048026:ginsenoside Rb2