title: What is the percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?
abstract: In this study, larvae (1,179 ex.) of mosquito genera Culex were examined for the presence of spirochaetes by Dark Field Microscopy (DFM) at the locality of Blansko (Czech Republic) in of 2004-2008. DFM spirochaete positive samples (25.4 %) were investigated by nested PCR; only 4 samples were positive for the presence of Borrelia burgdorferi sensu lato, which is 0.3 % of the total examined samples. We can conclude that only a low percentage of pathogenic borreliae are presented in mosquito larvae, while the spirochaete of undefined genera infect larvae in high amounts.
authors.affiliation: Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-61137 Brno, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Nejedla
authors.forename: Adam
authors.initials: A
authors.lastname: Norek
authors.forename: Karel
authors.initials: K
authors.lastname: Vostal
authors.forename: Alena
authors.initials: A
authors.lastname: Zakovska
mesh_terms: D000330:Aedes / Q000382:microbiology; D000818:Animals; D025065:Borrelia burgdorferi / Q000302:isolation & purification*; D003465:Culex / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D013997:Time Factors