title: Immunohistochemical investigation of wound healing in response to fractional photothermolysis.
abstract: Despite growing clinical evidence of ablative fractional photothermolysis (AFP), little is known about the spatiotemporal molecular changes within the targeted compartments. Six subjects received three different single AFP treatments using a scanned 250 mum CO(2)-laser beam. Spatiotemporal changes of skin regeneration were estimated by immunohistochemical investigation (HSP70, HSP72, HSP47, TGFbeta, procollagen III, CD3, CD20, and CD68) in skin samples 1 h, 3 days, and 14 days postintervention. The remodeling was uniformly started by regrowth of the epidermal compartment followed by partial to complete replacement of the microscopic ablation zones (MAZ) by newly synthesized condensed procollagen III. From day 3 to 14, the number of macrophages as well as giant cells surrounding the MAZ increased. TGFbeta expression was highest 1 h to 3 days following AFP. HSP70 and HSP72 expressions were highest 3-14 days postintervention in the spinocellular layer leading to an upregulation of HSP47. AFP performed by a scanned CO(2)-laser results in an early epidermal remodeling, which is followed by a dermal remodeling leading to a replacement of the MAZ with newly synthesized (pro)-collagen. During this, an inflammatory infiltrate with CD3(+) and CD20(+) cells surrounds the MAZ. The count of macrophages and giant cells involved in the replacement of the necrotic zones seems to be crucial for wound healing.
authors.affiliation: University of Leipzig, Department for Dermatology, Venerology, and Allergology, Philipp-Rosenthal-Strasse 23, Leipzig 04103 Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Helbig
authors.forename: Marc Oliver
authors.initials: MO
authors.lastname: Bodendorf
authors.forename: Sonja
authors.initials: S
authors.lastname: Grunewald
authors.forename: Michael
authors.initials: M
authors.lastname: Kendler
authors.forename: Jan C
authors.initials: JC
authors.lastname: Simon
authors.forename: Uwe
authors.initials: U
authors.lastname: Paasch
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D010782:Photolysis; D011347:Procollagen / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D006360:Heat-Shock Proteins; D011347:Procollagen; D016212:Transforming Growth Factor beta; C067980:CD68 antigen, human; D000097382:CD68 Molecule