Metal accumulation and metallothionein induction in the spotted dogfish Scyliorhinus canicula.
Recent studies indicate that elasmobranch fish respond differently to metal exposure than marine teleosts. Accumulation rates can be high, which despite the fact that normal background levels for metals in the marine environment are low, is worrying due to the long life span and late fecundity of most shark. The goals of the present study were to examine differences in accumulation rates and toxicity of a range of metals at equimolar concentrations (10microM) in the Mediterranean or spotted dogfish, Scyliorhinus canicula. For this purpose, we exposed the dogfish to Ni (587microg/L), Cd (1124microg/L), Pb (2072microg/L), Cu (635microg/L), and Ag (1079microg/L and two additional exposures at 10microg/L and 1microg/L) for one week and measured total metal accumulation, metallothionein induction, and parameters related to osmoregulation. Our study confirms the high toxicity and accumulation rates of Ag for elasmobranch fish, even at levels 100 to 1000 times lower than exposure levels of other metals. Also Pb accumulated readily in all organs, but did not cause any osmoregulatory disturbance at the exposure levels used. Ni and Cd seem to accumulate primarily in the kidney while Cu mainly accumulated in liver. In contrast to Ni and Cd, the three other metals Ag, Cu and Pb accumulated in the rectal gland, an important organ for osmoregulation and possible target organ for metal toxicity. Only Cu succeeded in initiating a protective response by inducing MT synthesis in liver and gills.
De Boeck
G
G
University of Antwerp, Department of Biology, Laboratory of Ecophysiology, Biochemistry, and Toxicology, Groenenborgerlaan 171, 2020 Antwerp, Belgium. gudrun.deboeck@ua.ac.be <gudrun.deboeck@ua.ac.be>
Eyckmans
M
M
Lardon
I
I
Bobbaers
R
R
Sinha
A K
AK
Blust
R
R
D000818:Animals; D001822:Body Burden; D019187:Cadmium Compounds / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004284:Dogfish / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D007854:Lead / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D008670:Metals / Q000378:metabolism* / Q000633:toxicity; D009532:Nickel / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D012835:Silver Nitrate / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity; D014882:Water-Electrolyte Balance / Q000187:drug effects
D019187:Cadmium Compounds; D029941:Fish Proteins; D008670:Metals; D009566:Nitrates; D014874:Water Pollutants, Chemical; D007854:Lead; C022838:nickel chloride; C017461:lead nitrate; D003300:Copper; D009532:Nickel; D008668:Metallothionein; D012835:Silver Nitrate; C516433:copper(II) nitrate; C035196:cadmium nitrate