Epidemiological study of hantavirus infection in the Samara Region of European Russia.
European Russia is a highly endemic area of hemorrhagic fever with renal syndrome (HFRS), a rodent-borne zoonotic disease, caused by hantaviruses. In total, 145 small mammals of four species (Myodes glareolus, Apodemus flavicollis, A. agrarius, and A. uralensis) were trapped in the Samara region of European Russia in August 2005 and examined for the presence of hantavirus (HV). Anti-HV antibodies were found in six of 68 (8.8%) M. glareolus and in one of 19 (5.3%) A. flavicollis by indirect immunofluorescent antibody assay (IFA). The Puumala virus (PUUV), which is one of the hantavirus species, was detected in the lungs of seven M. glareolus by RT-PCR. The virus S-segment was extremely similar (96.2% to 99.3%) to the sequence found in a fatal case of HFRS in the Samara region. Phylogenetic analyses of S and M segments showed that the Samara PUUVs form a cluster within the Russian Volga lineage and apparently differ from other European PUUVs. Anti-PUUV antibodies were found in blood sera from seven HFRS patients and from one undiagnosed patient from the Samara region, using IFA and an enzyme-linked immunosorbent assay (ELISA). These data suggest that the bank vole M. glareolus is a primary natural reservoir and vector for PUUV, which is the main causative agent of HFRS in humans in the Samara region.
Kariwa
Hiroaki
H
Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. kariwa@vetemed.hokudai.ac.jp
Tkachenko
Evgeniy A
EA
Morozov
Vyacheslav G
VG
Seto
Takahiro
T
Tanikawa
Yoichi
Y
Kolominov
Sergey I
SI
Belov
Sergey N
SN
Nakamura
Ichiro
I
Hashimoto
Nobuo
N
Balakiev
Alexander E
AE
Dzagurnova
Tamara K
TK
Daud
Nur Hardy Bin Abu
NH
Miyashita
Daisuke
D
Medvedkina
Olga A
OA
Nakauchi
Mina
M
Ishizuka
Mariko
M
Yoshii
Kentaro
K
Yoshimatsu
Kumiko
K
Arikawa
Jiro
J
Takashima
Ikuo
I
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003411:Arvicolinae / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D006476:Orthohantavirus / Q000235:genetics / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D029262:Puumala virus / Q000302:isolation & purification*; D012426:Russia / Q000453:epidemiology
D000914:Antibodies, Viral