Regulation of 11beta-hydroxysteroid dehydrogenase 1 and 2 by IGF-1 in mice.
Two isoforms of 11beta-hydroxysteroid dehydrogenase (11beta-HSD1 and 11beta-HSD2) play an important role in regulation of glucocorticoid corticosterone (CORT, the active form in rodents) by the interconversion between CORT and 11-dehydrocorticosterone (11DHC, the biologically inert form). 11beta-HSD1 is an NADP+/NADPH-dependent oxidoreductase which is mainly expressed in liver and kidney, while 11beta-HSD2 is an NAD+-dependent oxidase which is predominantly expressed in kidney. The regulation of 11beta-HSD1 and 11beta-HSD2 mRNA (Hsd11b1 and Hsd11b2) levels and their activities by IGF-1 was performed in liver, kidney, and testis of IGF-1 knockout male mice. Real-time PCR showed that Hsd11b1 in liver was decreased while Hsd11b2 mRNA level was decreased in kidney of IGF-1 null mice. 11beta-HSD1 and 11beta-HSD2 activities fluctuated with the changes of their respective Hsd11b1 or Hsd11b2 mRNA levels. In conclusion, IGF-I tissue-specifically regulates Hsd11b1 and Hsd11b2 expression.
Huang
Yadong
Y
Institutes of Life and Health Engineering, Jinan University, Guangzhou, Guangdong 510632, PR China.
Li
Xingwang
X
Lin
Han
H
Chu
Yanhui
Y
Chen
Bingbing
B
Lian
Qingquan
Q
Ge
Ren-Shan
RS
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000096:biosynthesis* / Q000235:genetics; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013737:Testis / Q000201:enzymology*
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2