Nanoparticle formation of organic compounds with retained biological activity.
Many pharmaceuticals are formulated as powders to aid drug delivery. A major problem is how to produce powders having high purity, controlled morphology, and retained bioactivity. We demonstrate the use of supercritical carbon dioxide as an antisolvent for meeting this need for two model drug systems, quercetin, a sparingly soluble antioxidant, and short interfering RNA (siRNA), which can silence genes. In both cases we achieve retention of bioactivity as well as a narrow particle size distribution in which the particles are free of impurities.
Jacobson
Gunilla B
GB
Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, California 94305-5080, USA.
Shinde
Rajesh
R
McCullough
Rebecca L
RL
Cheng
Nicholas J
NJ
Creasman
Adam
A
Beyene
Amanuel
A
Hickerson
Robyn P
RP
Quan
Can
C
Turner
Charlotta
C
Kaspar
Roger L
RL
Contag
Christopher H
CH
Zare
Richard N
RN
D002245:Carbon Dioxide / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D053758:Nanoparticles; D009930:Organic Chemicals; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011208:Powders
D009930:Organic Chemicals; D004364:Pharmaceutical Preparations; D011208:Powders; D002245:Carbon Dioxide