Polymorphisms in gene encoding TRPV1-receptor involved in pain perception are unrelated to chronic pancreatitis.
BACKGROUND
The major clinical feature in chronic pancreatitis is pain, but the genetic basis of pancreatic pain in chronic pancreatitis is poorly understood. The transient receptor potential vanilloid receptor 1 (TRPV1) gene has been associated with pain perception, and genetic variations in TRPV1 may modify the presence and phenotype of chronic pancreatitis. The aim of our study was to investigate the genetic variation of TRPV1 in Dutch patients with chronic pancreatitis and healthy controls.


METHODS
We genotyped 4 SNPs (rs222749, rs222747, rs224534 and rs8065080) in 228 chronic pancreatitis-patients and 207 healthy controls by PCR, followed by restriction-fragment-length-polymorphism analysis and DNA sequencing. We generated 27 diplotypes and compared prevalence between patients and controls.


RESULTS
There was no significant difference in allele frequency of the 4 TRPV1 gene SNPs in patients with chronic pancreatitis and healthy controls. Distribution of diplotypes was not statistically significantly different between patients and controls.


CONCLUSIONS
TRPV1 diplotypes are not associated with chronic pancreatitis.
van Esch
Aura A J
AA
Department of Gastroenterology & Hepatology, Radboud University Nijmegen Medical Centre, The Netherlands. A.vanEsch@MDL.umcn.nl
Lamberts
Mark P
MP
te Morsche
René H M
RH
van Oijen
Martijn G H
MG
Jansen
Jan B M J
JB
Drenth
Joost P H
JP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009426:Netherlands; D010146:Pain / Q000235:genetics*; D050500:Pancreatitis, Chronic / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D050916:TRPV Cation Channels / Q000235:genetics*; D055815:Young Adult
D050916:TRPV Cation Channels; C494484:TRPV1 receptor