title: High prevalence of ST131 isolates producing CTX-M-15 and CTX-M-14 among extended-spectrum-beta-lactamase-producing Escherichia coli isolates from Canada.
abstract: Phenotypic and genotypic methods were used to characterize extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli isolated in 2007 from 11 different Canadian medical centers. Of the 209 ESBL-producing E. coli isolates tested, 148 (71%) produced CTX-M-15, 17 (8%) produced CTX-M-14, 5 (2%) produced CTX-M-3, and 1 produced CTX-M-27. Overall, 96 (46%) of the ESBL producers belonged to clonal complex ST131, with the highest prevalence in Brampton, Calgary, and Winnipeg. ST131 is an important cause of community onset urinary tract infections due to ESBL-producing E. coli across Canada.
authors.affiliation: Calgary Laboratory Services, Calgary, Alberta, Canada.
authors.forename: Gisele
authors.initials: G
authors.lastname: Peirano
authors.forename: David
authors.initials: D
authors.lastname: Richardson
authors.forename: Jana
authors.initials: J
authors.lastname: Nigrin
authors.forename: Allison
authors.initials: A
authors.lastname: McGeer
authors.forename: Vivian
authors.initials: V
authors.lastname: Loo
authors.forename: Baldwin
authors.initials: B
authors.lastname: Toye
authors.forename: Michelle
authors.initials: M
authors.lastname: Alfa
authors.forename: Colette
authors.initials: C
authors.lastname: Pienaar
authors.forename: Pamela
authors.initials: P
authors.lastname: Kibsey
authors.forename: Johann D D
authors.initials: JD
authors.lastname: Pitout
mesh_terms: D015373:Bacterial Typing Techniques; D002170:Canada / Q000453:epidemiology; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D014552:Urinary Tract Infections / Q000453:epidemiology* / Q000382:microbiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C456080:beta-lactamase CTX-M-14; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases