title: Chronic morphine administration delays wound healing by inhibiting immune cell recruitment to the wound site.
abstract: Patients prescribed morphine for the management of chronic pain, and chronic heroin abusers, often present with complications such as increased susceptibility to opportunistic infections and inadequate healing of wounds. We investigated the effect of morphine on wound-healing events in the presence of an infection in an in vivo murine model that mimics the clinical manifestations seen in opioid user and abuser populations. We show for the first time that in the presence of an inflammatory inducer, lipopolysaccharide, chronic morphine treatment results in a marked decrease in wound closure, compromised wound integrity, and increased bacterial sepsis. Morphine treatment resulted in a significant delay and reduction in both neutrophil and macrophage recruitment to the wound site. The delay and reduction in neutrophil reduction was attributed to altered early expression of keratinocyte derived cytokine and was independent of macrophage inflammatory protein 2 expression, whereas suppression of macrophage infiltration was attributed to suppressed levels of the potent macrophage chemoattractant monocyte chemotactic protein-1. When the effects of chronic morphine on later wound healing events were investigated, a significant suppression in angiogenesis and myofibroblast recruitment were observed in animals that received chronic morphine administration. Taken together, our findings indicate that morphine treatment results in a delay in the recruitment of cellular events following wounding, resulting in a lack of bacterial clearance and delayed wound closure.
authors.affiliation: Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Josephine L
authors.initials: JL
authors.lastname: Martin
authors.forename: Lisa
authors.initials: L
authors.lastname: Koodie
authors.forename: Anitha G
authors.initials: AG
authors.lastname: Krishnan
authors.forename: Richard
authors.initials: R
authors.lastname: Charboneau
authors.forename: Roderick A
authors.initials: RA
authors.lastname: Barke
authors.forename: Sabita
authors.initials: S
authors.lastname: Roy
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D002464:Cell Migration Inhibition / Q000187:drug effects; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology; D004334:Drug Administration Schedule; D004353:Drug Evaluation, Preclinical; D007111:Immunity, Cellular / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000276:immunology* / Q000473:pathology
chemical_list: D000701:Analgesics, Opioid; D009020:Morphine