Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli.
Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.
Jiang
Min
M
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xinmofan Road #5, Nanjing 210009, China. bioengine@njut.edu.cn
Liu
Shu-Wen
SW
Ma
Jiang-Feng
JF
Chen
Ke-Quan
KQ
Yu
Li
L
Yue
Fang-Fang
FF
Xu
Bing
B
Wei
Ping
P
D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*
D001426:Bacterial Proteins; D006038:Glyoxylates; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D019802:Succinic Acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose