Cationic amphiphile with shikimic acid headgroup shows more systemic promise than its mannosyl analogue as DNA vaccine carrier in dendritic cell based genetic immunization.
Mannosylated cationic vectors have been previously used for delivering DNA vaccines to antigen presenting cells (APCs) via mannose receptors expressed on the cell surface of APCs. Here we show that cationic amphiphiles containing mannose-mimicking quinic acid and shikimic acid headgroups deliver genes to APCs via mannose receptor. Cationic amphiphile with shikimic acid headgroup was more efficacious than its mannosyl counterpart in combating mouse tumor growth by dendritic cell (the most professional APC) based genetic immunization.
Srinivas
Ramishetti
R
Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad 500 007, India.
Karmali
Priya P
PP
Pramanik
Dipankar
D
Garu
Arup
A
Mahidhar
Yenugonda Venkata
YV
Majeti
Bharat K
BK
Ramakrishna
Sistla
S
Srinivas
Gunda
G
Chaudhuri
Arabinda
A
D000818:Animals; D000938:Antigen-Presenting Cells; D002412:Cations / Q000737:chemistry*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D037181:Lectins, C-Type / Q000378:metabolism; D008081:Liposomes; D008358:Mannose / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D013058:Mass Spectrometry; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D011956:Receptors, Cell Surface / Q000378:metabolism; D012765:Shikimic Acid / Q000737:chemistry*; D019444:Vaccines, DNA / Q000008:administration & dosage*
D002412:Cations; D037181:Lectins, C-Type; D008081:Liposomes; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D019444:Vaccines, DNA; D012765:Shikimic Acid; D008358:Mannose