Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice.
This study evaluates Cryptosporidium parvum oocyst production in dexamethasone suppressed CF-1 and C57BL/6 mice. Both models can yield 1 x 10(9) total oocysts over a 20-day production period; however, only 20 CF-1 mice are required to reliably achieve this goal compared to 40 C57BL/6 mice. Although oocyst yields per mouse are similar for both mouse strains, the survival rate for CF-1 mice is higher, resulting in reduced lost production time per study when compared to the C57BL/6 mice. This study presents a more efficient and cost effective dexamethasone suppressed murine model of propagating high concentrations of C. parvum oocysts.
Ware
Michael W
MW
U.S. Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, OH 45268-1320, USA. Ware.michael@epa.gov
Villegas
Eric N
EN
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D016785:Cryptosporidium parvum / Q000254:growth & development*; D003907:Dexamethasone / Q000008:administration & dosage*; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D033661:Oocysts / Q000254:growth & development; D010274:Parasitology / Q000191:economics / Q000379:methods*
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone