Comparison of Recombinant Proteins from Schistosoma japonicum for Schistosomiasis Diagnosis.
The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant proteins with the potential to be diagnostic antigens were compared to the native soluble egg antigen preparation by enzyme-linked immunosorbent assay (ELISA). We evaluated the potentials of the recombinant proteins for discriminating active from prior infections, as well as the therapeutic efficacy of the established ELISA technique.
Jin
Ya-mei
YM
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, People's Republic of China. yameijin@shvri.ac.cn
Lu
Ke
K
Zhou
Wei-Fang
WF
Fu
Zhi-Qiang
ZQ
Liu
Jin-Ming
JM
Shi
Yao-Jun
YJ
Li
Hao
H
Lin
Jiao-Jiao
JJ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000276:immunology*; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins