Butin reduces oxidative stress-induced mitochondrial dysfunction via scavenging of reactive oxygen species.
This study investigated the cytoprotective effect of butin, a flavonoid, on hydrogen peroxide (H(2)O(2))-induced mitochondrial dysfunction. Electron spin resonance (ESR) spectrometry revealed butin's significant scavenging effects on superoxide radicals and hydroxyl radicals. When H(2)O(2) was used to induce an increase in mitochondrial reactive oxygen species (ROS) in Chinese hamster lung fibroblast (V79-4) cells, butin treatment decreased high level of ROS. Butin also attenuated intracellular Ca(2+) levels that have been induced by H(2)O(2). Furthermore, butin recovered ATP levels and succinate dehydrogenase activity that had been decreased by H(2)O(2) treatment. We conclude these results suggest butin decreased mitochondrial ROS accumulation, balanced intracellular Ca(2+) levels, and improved mitochondrial energy production, thus recovering mitochondrial function.
Zhang
Rui
R
School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, Republic of Korea.
Kang
Kyoung Ah
KA
Piao
Mei Jing
MJ
Chang
Weon Young
WY
Maeng
Young Hee
YH
Chae
Sungwook
S
Lee
In Kyung
IK
Kim
Bum Joon
BJ
Hyun
Jin Won
JW
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D003281:Contraceptives, Postcoital / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D001578:Benzopyrans; D003281:Contraceptives, Postcoital; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; C051437:butin; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase; D002118:Calcium