title: Cyclopamine and quercetin suppress the growth of leukemia and lymphoma cells.
abstract: BACKGROUND
  Hedgehog (Hh) and Wnt signaling pathways are involved in the stimulation of growth of leukemia and lymphoma cells. In the present study, whether or not the Hh inhibitor, cyclopamine, and the Wnt inhibitor, quercetin, suppress cell growth was investigated.
  
  
  METHODS
  The effects of cyclopamine and quercetin on the in vitro growth and protein expression of ten acute leukemia and B-cell lymphoma cell lines were examined.
  
  
  RESULTS
  Cyclopamine and quercetin suppressed cell growth and induced apoptosis in seven and eight cell lines respectively. Cyclopamine decreased the level of Gli1 protein, a target gene product of Hh signaling. Quercetin decreased the level of Notch1 protein and its active fragment in the DND-41 T-lymphoblastic leukemia cell line with constitutive Notch activation.
  
  
  CONCLUSIONS
  Cyclopamine and quercetin suppress the growth of a number of leukemia and lymphoma cells. This finding suggests the potential use of these compounds in molecularly-targeted therapy for leukemia and lymphoma.
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kawahara
authors.forename: Noriko
authors.initials: N
authors.lastname: Kawaguchi-Ihara
authors.forename: Yuki
authors.initials: Y
authors.lastname: Okuhashi
authors.forename: Mai
authors.initials: M
authors.lastname: Itoh
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nara
authors.forename: Shuji
authors.initials: S
authors.lastname: Tohda
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008223:Lymphoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014176:Protein Biosynthesis / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D051881:Receptor, Notch1 / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis; D014704:Veratrum Alkaloids / Q000494:pharmacology*; D000071676:Zinc Finger Protein GLI1
chemical_list: C400551:GLI1 protein, human; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D014157:Transcription Factors; D014704:Veratrum Alkaloids; D000071676:Zinc Finger Protein GLI1; D011794:Quercetin; C000541:cyclopamine