[Application of immunomagnetic separation to E. coli O157 : H7 detection].
In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1 : 6.34, 1 : 12.69, 1 : 25.38 and 1 : 30.07) and three different proportions of E. coli O157 : H7 and Bacillus subtilis (0.3 mL : 0.1 mL, 0.2 mL : 0.2 mL and 0.1 mL : 0.3 mL) were compared, with the total volume and capacity of IMB remaining constant. It was revealed that the capture efficiency could reach 100% when the proportion of E. coli O157 : H7 and IMB was 1 : 30, meaning that almost all targets E. coli O157 : H7 could be captured at 1 : 30, and that the capture efficiency decreased and then increased with the number of Bacillus subtilis increasing. Moreover, the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods, R2 = 0.9882, and the detection limit was as low as 10(2) CFU x mL(-1).
Tang
Qian-Qian
QQ
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China. tangqq252@163.com
Wang
Jian-Ping
JP
Ye
Zun-Zhong
ZZ
Gai
Ling
L
Ying
Yi-Bin
YB
D019453:Escherichia coli O157 / Q000302:isolation & purification*; D018189:Immunomagnetic Separation / Q000379:methods*