title: Candida albicans CaPTC6 is a functional homologue for Saccharomyces cerevisiae ScPTC6 and encodes a type 2C protein phosphatase.
abstract: Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ions. There are seven PP2C genes, ScPTC1, ScPTC2, ScPTC3, ScPTC4, ScPTC5, ScPTC6 and ScPTC7, in Saccharomyces cerevisiae. PTC6 is highly conserved in pathogenic and nonpathogenic yeasts. In the current study we have demonstrated that the Candida albicans CaPTC6 gene could complement the functions of ScPTC6 in the rapamycin and caffeine sensitivities of S. cerevisiae cells, indicating that they are functional homologues. We have also demonstrated that the CaPTC6-encoded protein is a typical PP2C enzyme and that CaPtc6p is localized in the mitochondrion of yeast-form and hyphal cells. However, deletion of CaPTC6 neither affects cell and hyphal growth nor renders Candida cells sensitive to rapamycin and caffeine. Therefore, possibly with a functional redundancy to other mitochondrial phosphatases, CaPtc6p is likely to be involved in the regulation of a mitochondrial physiology.
authors.affiliation: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Liquan
authors.initials: L
authors.lastname: Yu
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhao
authors.forename: Jinrong
authors.initials: J
authors.lastname: Feng
authors.forename: Jiaping
authors.initials: J
authors.lastname: Fang
authors.forename: Chen
authors.initials: C
authors.lastname: Feng
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Cao
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D002176:Candida albicans / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D025301:Hyphae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000071636:Protein Phosphatase 2C; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C518822:PTC6 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C