Two-color, two-photon uncaging of glutamate and GABA.
We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.
Kantevari
Srinivas
S
Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
Matsuzaki
Masanori
M
Kanemoto
Yuya
Y
Kasai
Haruo
H
Ellis-Davies
Graham C R
GC
D000818:Animals; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000502:physiology*; D008029:Lighting / Q000379:methods*; D008564:Membrane Potentials / Q000502:physiology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid