title: Simplified DGS procedure for large-scale genome structural study.
abstract: Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the original protocol by replacing the in vivo genomic DNA cloning with in vitro adaptor ligation, eliminating the ditag concatemerization steps, and replacing the 454 sequencer with Solexa or SOLiD sequencers for ditag sequence collection. This revised protocol further increases genome coverage and resolution and allows DGS to be used to analyze multiple genomes simultaneously.
authors.affiliation: Center for Functional Genomics, ENH Research Institute, Northwestern University, 1001 University Place, Evanston, IL 60201, USA.
authors.forename: Yong-Chul
authors.initials: YC
authors.lastname: Jung
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Yeong
authors.initials: Y
authors.lastname: Kim
authors.forename: David
authors.initials: D
authors.lastname: Winchester
authors.forename: San Ming
authors.initials: SM
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D002874:Chromosome Mapping / Q000379:methods*; D020224:Expressed Sequence Tags; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*