title: Detection of Notch signaling molecules in cemento-ossifying fibroma of the jaws.
abstract: BACKGROUND
  The aim of this study was to evaluate the roles of Notch signaling in the oncogenesis and cytodifferentiation of cemento-ossifying fibroma, the expressions of Notch receptors and ligands were detected in COF and normal jaw bones.
  
  
  METHODS
  The expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively in 16 cases of normal bone tissues and 12 cases of COF of the jaws.
  
  
  RESULTS
  The mRNAs expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected in all specimens. The expression levels of mRNAs in COF were higher than those in normal bones. In COF, Notch proteins staining were showed extensively distribution in fibroblasts and osteoblasts. In normal bone tissue, Notch proteins were expressed in osteoblasts, whereas proteins staining were weaker than those in COF, but no detection in fibroblast-like bone marrow stroma cells. The expressions of Notch receptors and ligands were not detected in cementum-like products or bone matrices.
  
  
  CONCLUSIONS
  Our data suggest that Notch signaling may participate in controlling cell differentiation and proliferation in normal bone and COF of the jaws. Notch signaling disorder may be a molecular incident in COF occurrence and development.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Tong-Han
authors.initials: TH
authors.lastname: Zhang
authors.forename: Hai-Chao
authors.initials: HC
authors.lastname: Liu
authors.forename: Gui-Qing
authors.initials: GQ
authors.lastname: Liao
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liang
authors.forename: Mei
authors.initials: M
authors.lastname: Chu
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Wan
authors.forename: Li-Zhong
authors.initials: LZ
authors.lastname: Liang
authors.forename: Guang-Sen
authors.initials: GS
authors.lastname: Zheng
mesh_terms: D001854:Bone Marrow Cells / Q000473:pathology; D001857:Bone Matrix / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D007568:Jaw / Q000473:pathology; D007573:Jaw Neoplasms / Q000473:pathology*; D008565:Membrane Proteins / Q000032:analysis*; D009808:Odontogenic Tumors / Q000473:pathology*; D010006:Osteoblasts / Q000473:pathology; D051881:Receptor, Notch1 / Q000032:analysis*; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000032:analysis*; D000072099:Serrate-Jagged Proteins; D017154:Stromal Cells / Q000473:pathology
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C490504:JAG2 protein, human; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; C499374:NOTCH3 protein, human; D051881:Receptor, Notch1; D000071656:Receptor, Notch3; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins