Protective effect of an herbal preparation (HemoHIM) on radiation-induced intestinal injury in mice.
The protective properties of an herbal preparation (HemoHIM) against intestinal damage were examined by evaluating its effects on jejunal crypt survival, morphological changes, and apoptosis in gamma-irradiated mice. The mice were whole-body irradiated with 12 Gy for the examination of jejunal crypt survival and any morphological changes and with 2 Gy for the detection of apoptosis and Ki-67 labeling. Irradiation was conducted using (60)Co gamma-rays. HemoHIM treatment was administered intraperitonially at a dosage of 50 mg/kg of body weight at 36 and 12 hours pre-irradiation and 30 minutes post-irradiation or orally at a dosage of 250 mg/kg of body weight/day for 7 or 11 days before necropsy. The HemoHIM-treated group displayed a significant increase in survival of jejunal crypts, when compared to the irradiation controls. HemoHIM treatment decreased intestinal morphological changes such as crypt depth, villus height, mucosal length, and basal lamina length of 10 enterocytes after irradiation. Furthermore, the administration of HemoHIM protected intestinal cells from irradiation-induced apoptosis. These results suggested that HemoHIM may be therapeutically useful to reduce intestinal injury following irradiation.
Kim
Sung Ho
SH
College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Lee
Hae June
HJ
Kim
Joong Sun
JS
Moon
Changjong
C
Kim
Jong Choon
JC
Park
Hae-Ran
HR
Jung
Uhee
U
Jang
Jong Sik
JS
Jo
Sung Kee
SK
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005720:Gamma Rays; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000293:injuries* / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D028321:Plant Preparations / Q000008:administration & dosage*; D011832:Radiation Injuries / Q000188:drug therapy* / Q000473:pathology; D011837:Radiation-Protective Agents / Q000008:administration & dosage*
D028321:Plant Preparations; D011837:Radiation-Protective Agents