[Migration of intravenously injected adipose tissue-derived stem cells in SD rats with soft tissue wound].
OBJECTIVE
To explore the migration of intravenously injected adipose tissue-derived stem cells (ADSCs) in SD rats with soft tissue wound.


METHODS
1.8 cm x 1.8 cm full thickness skin defect and 0.5 cm in depth soft tissue defects were made on the back of 6 SD rats. ADSCs were isolated and cultured in vitro for 3 passages. 2.4 x 10(6) ADSCs were labeled with DiI and transplanted into the SD rats through tail vein. Normal skin and wound tissue samples were collected for fluorescent distribution observation 24, 48 days after injection, respectively.


RESULTS
Compared to normal skin, more fluorescent positive cells were detected in the margin and deep layer of the wound 24 days after operation. But it is accumulated within dermis and adenoid 48 days after operation.


CONCLUSIONS
Wound can probably induce the migration and accumulation of intravenously transplanted ADSCs.
Nan
Hua
H
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Gao
Jian-Hua
JH
Lu
Feng
F
Liao
Yun-Jun
YJ
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002465:Cell Movement; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014945:Wound Healing; D014947:Wounds and Injuries