Amperometric catechol biosensor based on polyaniline-polyphenol oxidase.
A novel catechol biosensor was described based on the immobilization of polyphenol oxidase (PPO) into polyaniline (PANI), which was easily constructed by direct electropolymerization of aniline in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate (EMIES). The developed biosensor for the detection of catechol has a linear range of 1.25-150 micromol dm(-3). The maximum response current (I(max)) and the Michaelis-Menten constant (k'(m)) are 0.62 microA and 146 micromol dm(-3), respectively. The activation energy (E(a)) of the PPO catalytic reaction is 31.1 kJ mol(-1) in the B-R buffer. The biosensor shows good reproducibility (a relative standard deviation of 3.1% was obtained) and remarkable long-term stability (it retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described.
Tan
Yongyan
Y
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Guo
Xiaoxia
X
Zhang
Jinghui
J
Kan
Jinqing
J
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004156:Catechol Oxidase / Q000737:chemistry*; D002396:Catechols / Q000032:analysis* / Q000737:chemistry; D017076:Computer-Aided Design; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002396:Catechols; D004800:Enzymes, Immobilized; D010455:Peptides; C019529:polyalanine; D004156:Catechol Oxidase; C034221:catechol