Mobilization of CD133(+)CD34(-) cells in healthy individuals following whole-body acupuncture for spinal cord injuries.
Acupuncture can alleviate symptoms of spinal cord injuries (SCI). The underlying mechanism, however, is unknown. We hypothesized that stem cells could be mobilized by acupuncture. Therefore, we enrolled 14 healthy study participants using acupuncture points for the treatment of SCI. The frequency of CD133 and CD34 cells in peripheral blood and the serum concentrations of matrix metalloproteinase (MMP)-9, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and interleukin-6 were determined before and after acupuncture (<1 hr, 24 hr, and 48 hr). CD133(+)34(-) cells were doubled 48 hr after acupuncture, with concomitant decreases in BDNF and MMP-9 levels. Interleukin-6 remained below detectable levels, eliminating a stress-induced cell release. Individuals acupunctured on control counterpoints showed no changes in CD133(+) cells. Our results indicate that acupuncture for SCI can mobilize human CD133(+)34(-) cells. (c) 2009 Wiley-Liss, Inc.
Moldenhauer
Sonja
S
Clinic for Acupuncture, Erlangen, Germany.
Burgauner
Miriam
M
Hellweg
Rainer
R
Lun
Andreas
A
Hohenböken
Marina
M
Dietz
Ekkehart
E
Kiesewetter
Holger
H
Salama
Abdulgabar
A
Moldenhauer
Anja
A
D000071916:AC133 Antigen; D026881:Acupuncture; D015669:Acupuncture Points; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D001773:Blood Cells / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000097:blood; D010455:Peptides / Q000378:metabolism*; D013119:Spinal Cord Injuries; D055815:Young Adult
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D019208:Brain-Derived Neurotrophic Factor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D020932:Nerve Growth Factor; D020780:Matrix Metalloproteinase 9