Analysis of phenanthrene biodegradation by using FTIR, UV and GC-MS.
The aim of this paper is to assess the biodegradation of phenanthrene by Flavobacteria FCN2 which was isolated from coke plant sludge via a classical shaken liquid medium enrichment method. The strain FCN2 can decompose phenanthrene (50 mg l(-1)) completely within 5 days. The values of pH decrease to 6.7 from 7.2 during degradation periods. And a detailed phenanthrene metabolism was assayed by using FTIR, UV and GC-MS. For FTIR, appearance of new broad absorption bands at 2858 cm(-1), 2927 cm(-1), 2955 cm(-1) and another new strong absorption band at 1734 cm(-1) in metabolites demonstrates that carboxyl group produced during phenanthrene degradation. Besides this, a very strong absorption band appears at 1260 cm(-1). It is ascribed to C-C stretching vibration band in carbonyl group of arone. Two weak adsorption at 334 nm and 349 nm in UV spectra were assigned to the n-pi* transition of CO of aldehyde. Two metabolites, phenanthrene-dihydrodiol and naphthalene-1-diol were identified in neutral fraction of phenanthrene degradation by using GC-MS. As a result carboxylic acids and arone were generated during biodegradation of phenanthrene by Flavobacteria FCN2.
Wu
M L
ML
Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, China. wumanli@xauat.edu.cn
Nie
M Q
MQ
Wang
X C
XC
Su
J M
JM
Cao
W
W
D001673:Biodegradation, Environmental; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism; D005417:Flavobacterium / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010616:Phenanthrenes / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D002264:Carboxylic Acids; D010616:Phenanthrenes; C031181:phenanthrene