title: Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B.
abstract: Regulation of the E2F family of transcription factors is important in control of cellular proliferation; dysregulation of the E2Fs is a hallmark of many cancers. One member of the E2F family, E2F1, also has the paradoxical ability to induce apoptosis; however, the mechanisms underlying this selectivity are not fully understood. We now identify a nucleolar protein, RRP1B, as an E2F1-specific transcriptional target. We characterize the RRP1B promoter and demonstrate its selective response to E2F1. Consistent with the activation of E2F1 activity upon DNA damage, RRP1B is induced by several DNA-damaging agents. Importantly, RRP1B is required for the expression of certain E2F1 proapoptotic target genes and the induction of apoptosis by DNA-damaging agents. This activity is mediated in part by complex formation between RRP1B and E2F1 on selective E2F1 target gene promoters. Interaction between RRP1B and E2F1 can be found inside the nucleolus and diffuse nucleoplasmic punctates. Thus, E2F1 makes use of its transcriptional target RRP1B to activate other genes directly involved in apoptosis. Our data also suggest an underappreciated role for nucleolar proteins in transcriptional regulation.
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Paik
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.forename: Jerry K
authors.initials: JK
authors.lastname: Lue
authors.forename: Weei-Chin
authors.initials: WC
authors.lastname: Lin
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004249:DNA Damage; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D050687:E2F1 Transcription Factor; D009687:Nuclear Proteins; C544939:RRP1B protein, mouse