title: Paragonimus westermani: identification and characterization of the fasciclin I domain-containing protein.
abstract: Paragonimus westermani is a trematode parasite that causes inflammatory lung disease as well as systemic infections in carnivorous mammals. The interaction of the parasite with host cells and paired worms is initiated by adhesion and plays an important role in parasite proliferation and differentiation. In this study, we isolated a cDNA encoding a P. westermani fasciclin I domain-containing protein (Pwfas-I). The fasiclin-I domain is suggested to be involved in cell adhesion, migration, and differentiation. Immunohistochemical analysis of P. westermani adult worms with polyclonal anti-Pwfas-I serum revealed immunoreactivity in the egg shells and the cells lining the sub-tegumental layer of adult worm throughout the contact regions of the cyst wall and paired worms. Using cell adhesion and spreading assays, we showed that Pwfas-I supports cell adhesion and spreading. Furthermore, we determined that the alphanubeta5 integrin was a functional receptor for the Pwfas-I. Taken together, these results suggest that Pwfas-I may be functional for the modulation of cell adhesion via binding with alphanubeta5 integrin in the extracellular matrix of Paragonimus.
authors.affiliation: Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Song
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Shin
authors.forename: Jefferson V
authors.initials: JV
authors.lastname: de Guzman
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Hak-Sun
authors.initials: HS
authors.lastname: Yu
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Jha
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003400:Astacoidea; D001483:Base Sequence; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D048492:Paragonimus westermani / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D018076:DNA, Complementary; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins; C051641:fasciclin I