title: Silk fibroin nanoparticles for cellular uptake and control release.
abstract: Silk nanoparticles were prepared from silk fibroin solutions of domesticated Bombyx mori and tropical tasar silkworm Antheraea mylitta and investigated in respect to its particle size, surface charge, stability and morphology along with its cellular uptake and release of growth factors. The nanoparticles were stable, spherical, negatively charged, 150-170nm in average diameter and exhibited mostly Silk II (beta-sheet) structure and did not impose any overt toxicity. Cellular uptake studies showed the accumulation of fluorescence isothiocyanate conjugated silk nanoparticles in the cytosol of murine squamous cell carcinoma cells. In vitro VEGF release from the nanoparticles showed a significantly sustained release over 3 weeks, signifying the potential application as a growth factor delivery system.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India.
authors.forename: Joydip
authors.initials: J
authors.lastname: Kundu
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Chung
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Giyoong
authors.initials: G
authors.lastname: Tae
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000818:Animals; D012831:Bombyx; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004355:Drug Stability; D005349:Fibroins / Q000737:chemistry* / Q000633:toxicity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D051379:Mice; D009036:Moths; D053758:Nanoparticles; D010316:Particle Size; D047011:Silk / Q000737:chemistry / Q000633:toxicity; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*
chemical_list: D003692:Delayed-Action Preparations; D047011:Silk; D042461:Vascular Endothelial Growth Factor A; D005349:Fibroins; D016650:Fluorescein-5-isothiocyanate