title: Genotypes coding for low serum levels of mannose-binding lectin are underrepresented among individuals suffering from noninfectious systemic inflammatory response syndrome.
abstract: Gene polymorphisms, giving rise to low serum levels of mannose-binding lectin (MBL) or MBL-associated protease 2 (MASP2), have been associated with an increased risk of infections. The objective of this study was to assess the outcome of intensive care unit (ICU) patients with systemic inflammatory response syndrome (SIRS) regarding the existence of functionally relevant MBL2 and MASP2 gene polymorphisms. The study included 243 ICU patients with SIRS admitted to our hospital, as well as 104 healthy control subjects. MBL2 and MASP2 single nucleotide polymorphisms were genotyped using a sequence-based typing technique. No differences were observed regarding the frequencies of low-MBL genotypes (O/O and XA/O) and MASP2 polymorphisms between patients with SIRS and healthy controls. Interestingly, ICU patients with a noninfectious SIRS had a lower frequency for low-MBL genotypes and a higher frequency for high-MBL genotypes (A/A and A/XA) than either ICU patients with an infectious SIRS or healthy controls. The existence of low- or /high-MBL genotypes or a MASP2 polymorphism had no impact on the mortality rates of the included patients. The presence of high-MBL-producing genotypes in patients with a noninfectious insult is a risk factor for SIRS and ICU admission.
authors.affiliation: Emergency Department, Fundació Hospital de l'Esperit Sant, c/Avinguda Mossen Pons i Rabadà s/n, 08923 Santa Coloma Gramenet, Spain. asa30412@hotmail.com
authors.forename: Alex
authors.initials: A
authors.lastname: Smithson
authors.forename: Rafael
authors.initials: R
authors.lastname: Perello
authors.forename: Jesus
authors.initials: J
authors.lastname: Aibar
authors.forename: Gerard
authors.initials: G
authors.lastname: Espinosa
authors.forename: Dolors
authors.initials: D
authors.lastname: Tassies
authors.forename: Carolina
authors.initials: C
authors.lastname: Freire
authors.forename: Pedro
authors.initials: P
authors.lastname: Castro
authors.forename: Belen
authors.initials: B
authors.lastname: Suarez
authors.forename: Francisco
authors.initials: F
authors.lastname: Lozano
authors.forename: Josep-Maria
authors.initials: JM
authors.lastname: Nicolas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018746:Systemic Inflammatory Response Syndrome / Q000235:genetics*; D055815:Young Adult
chemical_list: D037601:Mannose-Binding Lectin; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases