Alterations in the ankyrin domain of TRPV4 cause congenital distal SMA, scapuloperoneal SMA and HMSN2C.
Spinal muscular atrophies (SMA, also known as hereditary motor neuropathies) and hereditary motor and sensory neuropathies (HMSN) are clinically and genetically heterogeneous disorders of the peripheral nervous system. Here we report that mutations in the TRPV4 gene cause congenital distal SMA, scapuloperoneal SMA, HMSN 2C. We identified three missense substitutions (R269H, R315W and R316C) affecting the intracellular N-terminal ankyrin domain of the TRPV4 ion channel in five families. Expression of mutant TRPV4 constructs in cells from the HeLa line revealed diminished surface localization of mutant proteins. In addition, TRPV4-regulated Ca(2+) influx was substantially reduced even after stimulation with 4alphaPDD, a TRPV4 channel-specific agonist, and with hypo-osmotic solution. In summary, we describe a new hereditary channelopathy caused by mutations in TRPV4 and present evidence that the resulting substitutions in the N-terminal ankyrin domain affect channel maturation, leading to reduced surface expression of functional TRPV4 channels.
Auer-Grumbach
Michaela
M
Institute of Human Genetics, Medical University of Graz, Austria. michaela.auergrumbach@medunigraz.at
Olschewski
Andrea
A
Papić
Lea
L
Kremer
Hannie
H
McEntagart
Meriel E
ME
Uhrig
Sabine
S
Fischer
Carina
C
Fröhlich
Eleonore
E
Bálint
Zoltán
Z
Tang
Bi
B
Strohmaier
Heimo
H
Lochmüller
Hanns
H
Schlotter-Weigel
Beate
B
Senderek
Jan
J
Krebs
Angelika
A
Dick
Katherine J
KJ
Petty
Richard
R
Longman
Cheryl
C
Anderson
Neil E
NE
Padberg
George W
GW
Schelhaas
Helenius J
HJ
van Ravenswaaij-Arts
Conny M A
CM
Pieber
Thomas R
TR
Crosby
Andrew H
AH
Guelly
Christian
C
D019943:Amino Acid Substitution / Q000235:genetics; D017089:Ankyrin Repeat; D002118:Calcium / Q000378:metabolism; D006367:HeLa Cells; D015417:Hereditary Sensory and Motor Neuropathy / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D042541:Intracellular Space / Q000378:metabolism; D015640:Ion Channel Gating; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009134:Muscular Atrophy, Spinal / Q000150:complications / Q000151:congenital* / Q000235:genetics* / Q000503:physiopathology; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D009995:Osmosis; D050916:TRPV Cation Channels / Q000737:chemistry* / Q000235:genetics*; D014162:Transfection
D050505:Mutant Proteins; D050916:TRPV Cation Channels; C416986:TRPV4 protein, human; D002118:Calcium