Inhibition of growth and motility of human A549 lung carcinoma cells by a recombined vascular basement membrane derived peptide.
We have previously constructed a recombined vascular basement membrane derived multifunctional peptide (rVBMDMP) which can inhibit tumor growth. The aim of this study is to explore the effects and mechanisms of rVBMDMP on growth and motility/invasion in human A549 lung carcinoma cells. The effect of rVBMDMP on A549 cell viability was determined by MTT assay while the motility/invasion was measured by scratch and transwell assays. Molecules that responded to rVBMDMP treatment of A549 cells were explored using the high-throughput Cancer Pathway Finder cDNA Microarray. We identified 16 genes that were up-regulated, including GZMA, ITG alphaV, MCAM and Kiss1 and 21 genes that were down-regulated, including uPA, uPAR, CDC25A, IGF1 and FGF2. Selective differentially expressed genes were further analyzed by real-time quantitative PCR and Western blot analysis. These findings contribute to the understanding of the molecular mechanisms mediating rVBMDMP action, and suggest that rVBMDMP is a promising novel agent for the treatment of human lung carcinoma.
Wang
Cheng-kun
CK
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan, China.
Cao
Jian-guo
JG
Peng
Bo
B
Gu
Yi-xue
YX
Zheng
Guo-pei
GP
He
Zhi-min
ZM
D001485:Basement Membrane / Q000737:chemistry*; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D010455:Peptides / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology
D010455:Peptides; D011994:Recombinant Proteins