Polymorphisms of TCF7L2 and HHEX genes in Chinese women with polycystic ovary syndrome.
OBJECTIVE
This study was to evaluate whether polymorphisms of TCF7L2 (rs7903146) and HHEX (rs1111875) genes responsible for insulin secretion are associated with the polycystic ovary syndrome (PCOS) in Chinese people.


METHODS
326 PCOS patients and 290 healthy individuals as controls were studied. Blood samples were obtained for DNA analyses and hormone measurements. Genotyping of the TCF7L2 (rs7903146) and HHEX (rs1111875) genes was carried out by the polymerase chain reaction-restriction fragment length polymorphism method.


RESULTS
We did not find statistically significant differences in the distribution of the TCF7L2 rs7903146 and HHEX rs1111875 polymorphisms between the Chinese women with PCOS and the controls. Levels of hormones such as insulin, FSH, LH, LH/FSH, P, T and E2 were also similar between the different genotypes of the genes TCF7L2 and HHEX, respectively, which was confirmed within either the PCOS subjects or controls.


CONCLUSIONS
There was no association of either of the two variants, rs7903146 of TCF7L2 and rs1111875 of HHEX, with the occurrence of PCOS in the Chinese population.
Xu
Pei
P
Jiangsu Key Laboratory of Molecular Medicine & The Center for Reproductive medicine, Drum Tower Hospital Affiliated to Medical School, Nanjing University, Nanjing, 210093, China.
Che
Yena
Y
Cao
Yunxia
Y
Wu
XiaoKe
X
Sun
Haixiang
H
Liang
Fengjing
F
Sun
Jing
J
Ke
Lu
L
Yi
Long
L
Wang
Yong
Y
D000328:Adult; D000704:Analysis of Variance; D044466:Asian People / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D042341:Gonadal Hormones / Q000097:blood; D006239:Haplotypes / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D010908:Pituitary Hormones, Anterior / Q000097:blood; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051820:TCF Transcription Factors / Q000235:genetics*; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors / Q000235:genetics*
D042341:Gonadal Hormones; C078492:HHEX protein, human; D018398:Homeodomain Proteins; D010908:Pituitary Hormones, Anterior; D051820:TCF Transcription Factors; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors