title: Near-infrared squaraine dyes for fluorescence enhanced surface assay.
abstract: Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed insurface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labelled compounds exhibited a 15 to 20-fold enhancement of fluorescence on the silver coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changes drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.
authors.affiliation: Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76106, USA.
authors.forename: Evgenia G
authors.initials: EG
authors.lastname: Matveeva
authors.forename: Ewald A
authors.initials: EA
authors.lastname: Terpetschnig
authors.forename: Megan
authors.initials: M
authors.lastname: Stevens
authors.forename: Leonid
authors.initials: L
authors.lastname: Patsenker
authors.forename: Olga S
authors.initials: OS
authors.lastname: Kolosova
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski