title: Effectiveness of inoculation with isolated Geobacillus strains in the thermophilic stage of vegetable waste composting.
abstract: An inoculum containing two amylolytic and three cellulolytic thermophilic bacteria, isolated from a preceding compost pile and identified as Geobacillus species by 16S rRNA gene sequencing, was applied to a mixture of market waste, rice straw and cow dung (5:1:0.2) so that the initial cell density was 2 x 10(8) colony forming unit (CFU) per gram dry weight at 55 degrees Celsius. The inoculation increased the total cell count particularly in the thermophilic stage as determined by flow cytometry. Concomitantly, there was a significant rise in microbial metabolism in the compost pile as reflected by the dehydrogenase activity. As a result, the C/N ratio dropped more rapidly in the inoculated mixture than that in the control without inoculum. The study, therefore, suggested that inoculation by thermophilic bacteria would be effective in the composting process at least in the thermophilic stage.
authors.affiliation: Agricultural and Ecological Research Unit, Indian Statistical Institute, 203 B.T. Road, Kolkata 700 108, India.
authors.forename: Sutripta
authors.initials: S
authors.lastname: Sarkar
authors.forename: Rajdeep
authors.initials: R
authors.lastname: Banerjee
authors.forename: Sunanda
authors.initials: S
authors.lastname: Chanda
authors.forename: Pradeep
authors.initials: P
authors.lastname: Das
authors.forename: Sandipan
authors.initials: S
authors.lastname: Ganguly
authors.forename: Subrata
authors.initials: S
authors.lastname: Pal
mesh_terms: D000818:Animals; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D002417:Cattle; D005434:Flow Cytometry; D056506:Geobacillus / Q000235:genetics*; D008372:Manure / Q000382:microbiology; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D012275:Oryza; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods*; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D014675:Vegetables
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002244:Carbon; D009584:Nitrogen