Lentiviral gene transduction in human and mouse NK cell lines.
Natural killer (NK) cells play a vital role in the control of cancer and microbial infections. A major hinderance in studying NK cells is the resistance of these cells to gene transfer. Considering over-expression and gene knockdown studies are crucial tools to study the biology of cells, technologies suitable for transferring genes into NK cells are invaluable. Among various technologies available for gene transfer, lentiviral-mediated transduction has been successful in introducing genes into NK cells. We have standardized methods of lentiviral infection in human and mouse NK cell lines. We obtain transduction efficiencies of 15% in the NK-92 cell line and 30-40% in LNK, YT, and DERL7 cell lines. This method allows efficient and stable introduction of genes and shRNAs into NK cell lines.
Savan
Ram
R
Laboratory of Experimental Immunology, Cancer and Inflammation Program, National Institutes of Health-Frederick, Frederick, MD, USA.
Chan
Tim
T
Young
Howard A
HA
D000818:Animals; D002460:Cell Line; D006801:Humans; D055029:Inverted Repeat Sequences; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000821:virology; D016086:Lentivirus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*; D019562:Viral Load
D012313:RNA