The mitogaligin protein is addressed to the nucleus via a non-classical localization signal.
Mitogaligin, a protein encoded by galig, an internal cytotoxic gene of the galectin-3 locus, is mostly a mitochondrial protein. Mitochondrial targeting is due to an already identified mitochondrial localization signal. Interaction of mitogaligin with mitochondria leads to cytochrome c cytosolic leakage and ultimately to cell death. We have previously pointed out that mitogaligin can also be directed to the nucleus when the mitochondrial addressing signal is inactivated, indicating a possible dual intracellular localization of the protein. When expressed in the nucleus, mitogaligin exhibits also apoptotic properties leading to cell death. In this report, we show that nuclear addressing of mitogaligin depends on a sequence differing from classical signals containing basic, lysine or proline-tyrosine rich residues. The signal consists of a long sequence of amino acids residues based on a series of a short repetitive degenerated sequence.
Robinet
Pauline
P
Centre de Biophysique Moléculaire (Affiliated with the University of Orléans), CNRS UPR4301, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
Mollet
Lucile
L
Gonzalez
Patrick
P
Normand
Thierry
T
Charpentier
Stéphane
S
Brulé
Fabienne
F
Dubois
Martine
M
Legrand
Alain
A
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D037161:Galectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion
D001798:Blood Proteins; D037161:Galectins; C428445:LGALS3 protein, human; D019913:Nuclear Localization Signals