Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region.
We present a three-color multiplex coherent anti-Stokes Raman scattering (CARS) setup that facilitates a prompt recording of broadband CARS spectra along with a fast CARS imaging. With separate narrowband Stokes and probe beams being introduced in the near IR, we are able to incorporate a stable, wideband Ti:sapphire femtosecond laser as a pump beam that covers the full range of Raman shift for CHn stretching vibrational modes. Experimentally, high-resolution multiplex CARS signals are allowed to investigate molecular vibrations over the range of 2650 cm-1 - 3050 cm-1, which are spectrally integrated to construct lipid-sensitive images. It is demonstrated that the proposed implementation promises a particular benefit on CARS imaging of lipid-rich tissue structures by providing detailed information on CHn Raman-active vibrations at points of interest on the CARS images that can be obtained at high frame rates.
Lee
Jae Yong
JY
Division of Convergence Technology, Korea Research Institute of Standards and Science, 1 Doryong-dong, Yuseong-gu, Daejeon 305-340, Korea. eslee@kriss.re.kr
Kim
Se-Hwa
SH
Moon
Dae Won
DW
Lee
Eun Seong
ES
D002244:Carbon / Q000737:chemistry*; D003124:Colorimetry / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006859:Hydrogen / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D014732:Vibration
D002244:Carbon; D006859:Hydrogen