title: Enzymatic reactions in near critical CO₂: The effect of pressure on phenol removal by tyrosinase.
abstract: The use of enzymes in supercritical CO(2) (SCCO(2)) has received extensive attention in recent years. Biocatalysts have the advantage of substrate specificity and SCCO(2) offers several advantages over liquid solvents. This work deals with the utilization of SCCO(2) as a medium for the enzymatic removal of phenol from aqueous solutions using tyrosinase. Since the presence of oxygen is crucial for the enzyme-catalyzed oxidation, the substantial solvating power of SCCO(2) makes it a promising medium for such reactions. The conversion of phenol was higher at 10 MPa. Under near critical conditions (7 MPa, 35 degrees C), the addition of air at 5 x 10(5) Pa of pressure improved phenol removal.
authors.affiliation: School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Amaral
authors.affiliation: School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Garcia
authors.affiliation: Chemical and Biological Engineering Department, Instituto Superior Técnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Cardoso
authors.affiliation: Chemical Engineering Department, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, 23890, Seropédica, RJ, Brazil.
authors.forename: Marisa
authors.initials: M
authors.lastname: Mendes
authors.affiliation: School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: Coelho
authors.affiliation: School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pessoa
mesh_terms: D000364:Agaricus / Q000201:enzymology; D002245:Carbon Dioxide / Q000737:chemistry*; D002384:Catalysis; D005656:Fungal Proteins / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D011312:Pressure
chemical_list: D005656:Fungal Proteins; D002245:Carbon Dioxide; D019800:Phenol; D014442:Monophenol Monooxygenase