Peritoneal infusion with cold saline decreased postoperative intra-abdominal adhesion formation.
BACKGROUND
Postoperative intra-abdominal adhesion is a common morbidity after laparotomy. We try to determine whether peritoneal infusion with cold saline may decrease postoperative intra-abdominal adhesion formation.


METHODS
Ninety-six male BALB/c mice weighing 25-30 g were randomized into four groups: (I) adhesion model with infusion of 4 degrees C cold saline, (II) adhesion model with infusion of room temperature saline, (III) adhesion model without infusion of saline, and (IV) sham operation without infusion of saline. Adhesion scores, incidence of adhesion, and serum cytokines were measured at postoperative days 1, 3, 7, and 14.


RESULTS
Group I had lower adhesion scores than groups II and III (P < 0.0001). IL-6, IL-10, and TNF-alpha were significantly increased in the groups I, II, and III compared to group IV (P < 0.0001). IL-6 in group I was significantly decreased compared to that in group III (P < 0.0004). IL-10 in group I was significantly increased compared to that in groups II (P < 0.0001) and III (P < 0.05). TNF-alpha in group I was significantly decreased compared to that in groups II (P < 0.0004), and III (P < 0.05).


CONCLUSIONS
Peritoneal infusion with cold saline may decrease the degree of postoperative intra-abdominal adhesion formation.
Fang
Cheng-Chung
CC
Department of Emergency Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, No. 7, Chung-Shan South Road, Taipei, 10016, Taiwan.
Chou
Tzung-Hsin
TH
Lin
Geng-Shiau
GS
Yen
Zui-Shen
ZS
Lee
Chien-Chang
CC
Chen
Shyr-Chyr
SC
D000704:Analysis of Variance; D000818:Animals; D003080:Cold Temperature; D016207:Cytokines / Q000097:blood; D007263:Infusions, Parenteral; D007813:Laparotomy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000601:surgery*; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D012965:Sodium Chloride / Q000008:administration & dosage*; D000267:Tissue Adhesions / Q000517:prevention & control*
D016207:Cytokines; D012965:Sodium Chloride