Toward engineering synthetic microbial metabolism.
The generation of well-characterized parts and the formulation of biological design principles in synthetic biology are laying the foundation for more complex and advanced microbial metabolic engineering. Improvements in de novo DNA synthesis and codon-optimization alone are already contributing to the manufacturing of pathway enzymes with improved or novel function. Further development of analytical and computer-aided design tools should accelerate the forward engineering of precisely regulated synthetic pathways by providing a standard framework for the predictable design of biological systems from well-characterized parts. In this review we discuss the current state of synthetic biology within a four-stage framework (design, modeling, synthesis, analysis) and highlight areas requiring further advancement to facilitate true engineering of synthetic microbial metabolism.
McArthur
George H
GH
Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284-3028, USA.
Fong
Stephen S
SS
D018407:Bacterial Physiological Phenomena; D032701:Biomimetics / Q000379:methods*; D003198:Computer Simulation; D005658:Fungi / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D008954:Models, Biological; D015398:Signal Transduction / Q000502:physiology*