Investigation on interaction of prulifloxacin with pepsin: a spectroscopic analysis.
The interaction between prulifloxacin, a kind of new oral taking antibiotic and pepsin, a kind of enzyme in the stomach has been investigated in vitro under a simulated physiological condition by different spectroscopic methods. The intrinsic fluorescence of pepsin was strongly quenched by prulifloxacin. This effect was rationalized in terms of a static quenching procedure. The binding parameters have been evaluated by fluorescence quenching methods. The negative value of DeltaG(0) reveals that the binding process is a spontaneous process. The binding distance R between donor (pepsin) and acceptor (prulifloxacin) was obtained according to the Förster's resonance energy transfer theory and found to be 0.95 nm. The results obtained herein will be of biological significance in pharmacology and clinical medicine.
Huang
Yabei
Y
Department of Chemistry, Huaibei Coal Normal College, Huaibei, Anhui Province, China.
Yan
Jie
J
Liu
Benzhi
B
Yu
Zhang
Z
Gao
Xiaoyan
X
Tang
Yingcai
Y
Zi
Yanqin
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D004148:Dioxolanes / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer / Q000502:physiology; D024841:Fluoroquinolones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D010434:Pepsin A / Q000737:chemistry / Q000378:metabolism; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D004148:Dioxolanes; D024841:Fluoroquinolones; D010879:Piperazines; D010434:Pepsin A; C074191:prulifloxacin