title: Whole-body, real-time preclinical imaging of quantum dot fluorescence with time-gated detection.
abstract: We describe a wide-field preclinical imaging system optimized for time-gated detection of quantum dot fluorescence emission. As compared to continuous wave measurements, image contrast was substantially improved by suppression of short-lifetime background autofluorescence. Real-time (8 frames/s) biological imaging of subcutaneous quantum dot injections is demonstrated simultaneously in multiple living mice.
authors.forename: Andrzej
authors.initials: A
authors.lastname: May
authors.forename: Srabani
authors.initials: S
authors.lastname: Bhaumik
authors.forename: Sanjiv S
authors.initials: SS
authors.lastname: Gambhir
authors.forename: Chun
authors.initials: C
authors.lastname: Zhan
authors.forename: Siavash
authors.initials: S
authors.lastname: Yazdanfar
mesh_terms: D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D045663:Quantum Dots; D051598:Whole Body Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes