Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells.
Adult mammalian neurogenesis occurs in the hippocampus and the olfactory bulb, whereas neocortical adult neurogenesis remains controversial. Several occurrences of neocortical adult neurogenesis in injured neocortex were recently reported, suggesting that neural stem cells (NSCs) or neuronal progenitor cells (NPCs) that can be activated by injury are maintained in the adult brain. However, it is not clear whether or where neocortical NSCs/NPCs exist in the brain. We found NPCs in the neocortical layer 1 of adult rats and observed that their proliferation was highly activated by global forebrain ischemia. Using retrovirus-mediated labeling of layer 1 proliferating cells with membrane-targeted green fluorescent protein, we found that the newly generated neurons were GABAergic and that the neurons were functionally integrated into the neuronal circuitry. Our results suggest that layer 1 NPCs are a source of adult neurogenesis under ischemic conditions.
Ohira
Koji
K
Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan. ohira@fujita-hu.ac.jp
Furuta
Takahiro
T
Hioki
Hiroyuki
H
Nakamura
Kouichi C
KC
Kuramoto
Eriko
E
Tanaka
Yasuyo
Y
Funatsu
Nobuo
N
Shimizu
Keiko
K
Oishi
Takao
T
Hayashi
Motoharu
M
Miyakawa
Tsuyoshi
T
Kaneko
Takeshi
T
Nakamura
Shun
S
D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D049109:Cell Proliferation; D008297:Male; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016548:Prosencephalon / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000503:physiopathology*; D055153:Stem Cell Niche / Q000503:physiopathology; D013569:Synapses / Q000502:physiology; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005680:gamma-Aminobutyric Acid