Novel pH-sensitive microgels prepared using salt bridge.
pH-sensitive microgels were prepared by crosslinking carboxymethylcellulose (CMC) and polymeric beta-cyclodextrin (PbetaCD) using (2-hydroxyethyl)trimethylammonium chloride benzoate (TMACB) as a crosslinker. PbetaCD was prepared by reacting epichlorohydrin and beta-CD in an aqueous phase (NaOH solution, 30% (w/w)). TMACB will interact with CMC by an electrostatic interaction and it will also interact with PbetaCD by a hydrophobic interaction. The size of microgel was tens of nanometers to several micrometers. The degree of calcein release in 24h from the microgels was as low as 23% at pH 8.0. The degree of release at pH 3.0 was almost 100%. The carboxyl groups of CMC will lose their charge in an acidic condition and they would lose their ability to form salt bridges with TMACB, leading to the disintegration of microgels. The degree of release at pH 11, about 47%, was less than the value at pH 3.0 but it was greater than the value at pH 8.0. The CMC will be strongly electrostatically charged in the alkali condition, so the microgels would swell due to the electrostatic repulsion among CMC molecules, which could promote the release of their contents.
Yang
Xia
X
School of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1 Hyoja 2 dong, Chunchon, Kangwon-do 200-701, Republic of Korea.
Kim
Jin-Chul
JC
D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005452:Fluoresceins / Q000008:administration & dosage*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010316:Particle Size; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D055672:Static Electricity; D047392:beta-Cyclodextrins / Q000737:chemistry
C528098:(2-hydroxyethyl) trimethylammonium hydroxide; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D005452:Fluoresceins; D005782:Gels; D000644:Quaternary Ammonium Compounds; D012492:Salts; D047392:beta-Cyclodextrins; C031215:betadex; D002266:Carboxymethylcellulose Sodium; C007740:fluorexon