title: Effects of static magnetic field exposure on antioxidative enzymes activity and DNA in rat brain.
abstract: The present study was undertaken in order to investigate the effects of static magnetic field (SMF) exposure on the antioxidative enzymes activity, malondialdehyde (MDA) concentration and DNA oxidation in male rat brain. The exposure of rats to SMF (128 mT, 1 h/day during 30 consecutive days) decreased the glutathione peroxidase (GPx; -39%, p < 0.05), CuZn superoxide dismutase (CuZn-SOD; -35%, p < 0.05) and catalase (-59%, p < 0.05) activities in frontal cortex. The same treatment decreased the CuZn-SOD (-51%, p < 0.05) and Mn-SOD (-13%, p < 0.05) activities in hippocampus. However, the glutathione levels remained unchanged in the both brain structures. In the hippocampus, SMF exposure increased MDA concentration (+32%, p < 0.05). Interestingly, exposed-rats to SMF displayed a significant increase of metallothioneins level in frontal cortex (+100%, p < 0.05), while the 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) concentration remained unaffected, indicating the absence of DNA oxidation. Our results indicated that sub-chronic exposure to SMF induced oxidative stress in rat hippocampus and frontal cortex. Metallothionein induction protected probably DNA against oxidative damage.
authors.affiliation: Laboratoire de Physiologie Animale, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia. amara_salem_fsb@yahoo.fr
authors.forename: Salem
authors.initials: S
authors.lastname: Amara
authors.forename: Thierry
authors.initials: T
authors.lastname: Douki
authors.forename: Catherine
authors.initials: C
authors.lastname: Garel
authors.forename: Alain
authors.initials: A
authors.lastname: Favier
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Khémais B
authors.initials: KB
authors.lastname: Rhouma
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000201:enzymology* / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004574:Electromagnetic Fields; D005625:Frontal Lobe / Q000201:enzymology / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D008668:Metallothionein; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine; D005978:Glutathione