New nucleotide analogues with enhanced signal properties.
We describe synthesis and testing of a novel type of dye-modified nucleotides which we call macromolecular nucleotides (m-Nucs). Macromolecular nucleotides comprise a nucleotide moiety, a macromolecular linear linker, and a large macromolecular ligand carrying multiple fluorescent dyes. With incorporation of the nucleotide moiety into the growing nucleic acid strand during enzymatic synthesis, the macromolecular ligand together with the coupled dyes is bound to the nucleic acid. By the use of this new class of modified nucleotides, signals from multiple dye molecules can be obtained after a single enzymatic incorporation event. The modified nucleotides are considered especially useful in the fields of nanobiotechnology, where signal stability and intensity is a limiting factor.
Cherkasov
Dmitry
D
Institute for Medical Microbiology, Philipps University of Marburg, Hans-Meerweinstrasse 2, 35043 Marburg, Germany. cherkasovd07@yandex.ru
Biet
Thorsten
T
Bäuml
Englbert
E
Traut
Walther
W
Lohoff
Michael
M
D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000378:metabolism; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D008024:Ligands; D036103:Nanotechnology / Q000379:methods*; D009711:Nucleotides / Q000032:analysis* / Q000737:chemistry*; D010799:Phycoerythrin / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000737:chemistry
D004798:Enzymes; D005456:Fluorescent Dyes; D008024:Ligands; D009711:Nucleotides; D010799:Phycoerythrin; D011092:Polyethylene Glycols; D019809:Streptavidin; D019793:Fluorescein