The effects of mixed MPEG-PLA/Pluronic copolymer micelles on the bioavailability and multidrug resistance of docetaxel.
A mixed micelle that comprised of MPEG-PLA (MPP) and Pluronic copolymers was developed for enhanced bioavailability and to overcome multidrug resistance of docetaxel in cancer therapy. The mixed micelles that sufficiently solubilized docetaxel were evaluated for the effect of Pluronic copolymers weight ratio on the mixed micelles with respect to drug loading and drug release. In vitro, cell viability and cytotoxicity studies in KB and KBv cells revealed that the mixed micellar formulations were more potent than the commercial docetaxel formulation (Taxotere). In vivo pharmacokinetics study in rats showed that the mixed micelles significantly enhanced the bioavailability of docetaxel (3.6 fold) than Taxotere. Moreover, antitumor activity assessed in KBv cancer xenograft BALB/C nude mice models showed that the mixed micelles significantly reduced the tumor size than the control (Taxotere). Clear differences in the intracellular uptake of docetaxel between MPP and mixed micelles were observed using confocal laser scanning microscopy. This study presents not only a new micelle structure for a diblock-triblock copolymer system, but also a method for enhanced bioavailability of docetaxel and to overcome some of the limitations on its multidrug resistance in cancer therapy.
Mu
Chao-Feng
CF
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Balakrishnan
Prabagar
P
Cui
Fu-De
FD
Yin
Yong-Mei
YM
Lee
Yong-Bok
YB
Choi
Han-Gon
HG
Yong
Chul Soon
CS
Chung
Suk-Jae
SJ
Shim
Chang-Koo
CK
Kim
Dae-Duk
DD
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects*; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems; D018432:Drug Resistance, Multiple; D006801:Humans; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D009368:Neoplasm Transplantation; D020442:Poloxamer / Q000737:chemistry / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism; D043823:Taxoids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; D013501:Surface-Active Agents; D043823:Taxoids; C492425:methoxy poly(ethylene glycol)-poly(lactide); D020442:Poloxamer; D000077143:Docetaxel; D011092:Polyethylene Glycols