title: Cytokeratin 8/18 is a useful immunohistochemical marker for hepatocellular proliferative lesions in mice.
abstract: In order to clarify whether cytokeratin (CK) 8/18 is a useful immunohistochemical marker for hepatocellular proliferative lesions in mice, partially hepatectomized male ICR mice were given 0.6% piperonyl butoxide (PBO) for 8 (Experiment I) or 25 weeks (Experiment II) after N-diethylnitrosamine (DEN) initiation treatment, and the livers were subjected to histological examinations on hematoxylin and eosin (HE) stained sections, CK8/18 immunohistochemistry and gamma-glutamyl transpeptidase (GGT) histochemistry. In Experiment I, the multiplicity of hepatocellular foci in paraffin-embedded sections which were observed in HE-stained sections and positive for CK8/18 was 10.17 and 18.50, respectively, while that of hepatocellular foci in frozen sections which were observed in HE-stained sections and positive/negative for GGT was 6.17 and 8.17, respectively. In Experiment II, the total multiplicity of hepatocellular foci in paraffin-embedded sections which were observed in HE-stained sections and positive/negative for CK8/18 was 4.47 and 23.17, respectively, while that of hepatocellular foci in frozen sections which were observed in HE-stained sections and positive/negative for GGT was 2.50 and 3.50, respectively. Most of the hepatocellular adenomas and carcinomas observed in HE-stained sections were positive for CK8/18, but some of the adenomas were negative for CK8/18. These findings indicate that more hepatocellular proliferative lesions can be detected in CK8/18 immunohistochemistry in addition to those observed in HE-stained sections, and suggest that CK8/18 may become a useful immunohistochemical marker for detecting hepatocellular proliferative lesions in mice.
authors.affiliation: Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Masaomi
authors.initials: M
authors.lastname: Kawai
authors.forename: Yukie
authors.initials: Y
authors.lastname: Saegusa
authors.forename: Sayaka
authors.initials: S
authors.lastname: Kemmochi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Harada
authors.forename: Keisuke
authors.initials: K
authors.lastname: Shimamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Shibutani
authors.forename: Kunitoshi
authors.initials: K
authors.lastname: Mitsumori
mesh_terms: D000236:Adenoma / Q000139:chemically induced / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002277:Carcinoma / Q000139:chemically induced / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000473:pathology*; D004052:Diethylnitrosamine; D006498:Hepatectomy; D007150:Immunohistochemistry; D053538:Keratin-18 / Q000032:analysis*; D053533:Keratin-8 / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009367:Neoplasm Staging; D010882:Piperonyl Butoxide; D051381:Rats
chemical_list: D015415:Biomarkers; D053538:Keratin-18; D053533:Keratin-8; D004052:Diethylnitrosamine; D010882:Piperonyl Butoxide