title: Effect of Tisseel on bone healing with particulate dentin and plaster of Paris mixture.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of Tisseel on the early healing of bone defects in the skulls of rats.
  
  
  METHODS
  Forty-eight rats were randomly assigned to 4 groups, and each group was further divided into 3 subgroups which were examined at 4 and 8 weeks after the defects were filled. The 4 different groups were animals containing: no graft (group 1); particulate dentin and plaster of Paris mixture graft (group 2); Tisseel and particulate dentin and plaster of Paris mixture graft (group 3); and Tisseel graft (group 4). After killing the animals at 4 and 8 weeks after surgery, all implant blocks were prepared for histologic sections and histomorphometric analysis.
  
  
  RESULTS
  The overall new bone formation was significantly different between the 4-week samples and the 8-week samples. Significant differences between groups 1 and 2, groups 1 and 3, groups 1 and 4, groups 2 and 4, and groups 3 and 4 were observed at 4 weeks after surgery. At 8 weeks after surgery, significant differences between groups 1 and 2, groups 1 and 3, groups 1 and 4, and groups 2 and 4 were observed. As expected, effective bone formation was observed when the defects were filled with either particulate dentin, Tisseel, or particulate dentin-Tisseel combination. It was also observed from this study that particulate dentin is especially effective, followed by the particulate dentin-Tisseel combination and Tisseel.
  
  
  CONCLUSIONS
  It was concluded that the use of Tisseel may be an alternative therapy for regenerating bone in defects when used in combination with particulate dentin.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Gwangju, Korea.
authors.forename: Wan-Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Lim
authors.forename: Young-Kyun
authors.initials: YK
authors.lastname: Kim
authors.forename: Si-Nae
authors.initials: SN
authors.lastname: Park
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology; D002133:Calcium Sulfate / Q000494:pharmacology; D003804:Dentin / Q000637:transplantation*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D020007:Implants, Experimental; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000601:surgery*; D014014:Tissue Adhesives / Q000494:pharmacology
chemical_list: D018786:Bone Substitutes; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D002133:Calcium Sulfate