title: An extract based on the medicinal mushroom Agaricus blazei Murill stimulates monocyte-derived dendritic cells to cytokine and chemokine production in vitro.
abstract: The edible mushroom Agaricus blazei Murill (AbM), which has been used in traditional medicine against a range of diseases and possess immunomodulating properties, probably due to its high content of beta-glucans. Others and we have demonstrated stimulatory effects of extracts of this mushroom on different immune cells. Dendritic cells are major directors of immune function. We wanted to examine the effect of AbM stimulation on signal substance release from monocyte-derived dendritic cells (MDDC). After 6d incubation with IL-4 and GM-CSF, the cells were true MDDC. Then the cells were further incubated with up to 10% of the AbM-based extract, AndoSan, LPS (0.5 microg/ml) or PBS control. We found that the AbM extract promoted dose-dependent increased levels of IL-8, G-CSF, TNFalpha, IL-1beta, IL-6 and MIP-1beta, in that order. The synthesis of IL-2, IL-8 and IFNgamma were similar for the AbM extract and LPS. However, AndoSan induced a 10- to 2-fold higher production than did LPS of G-CSF, TNFalpha and IL-1beta, respectively. AbM did not induce increased synthesis of Th2 or anti-inflammatory cytokines or the Th1 cytokine IL-12. We conclude that stimulation of MDDC with an AbM-based extract resulted in increased production of proinflammatory, chemotactic and some Th1-type cytokines in vitro.
authors.affiliation: Department of Gastroenterological Surgery, Oslo University Hospital, Ulleval, Norway.
authors.forename: D T
authors.initials: DT
authors.lastname: Førland
authors.forename: E
authors.initials: E
authors.lastname: Johnson
authors.forename: A M A
authors.initials: AM
authors.lastname: Tryggestad
authors.forename: T
authors.initials: T
authors.lastname: Lyberg
authors.forename: G
authors.initials: G
authors.lastname: Hetland
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008519:Medicine, Traditional; D009000:Monocytes / Q000166:cytology / Q000502:physiology
chemical_list: D002457:Cell Extracts; D018925:Chemokines; D016207:Cytokines