Effects of acrylamide on the activity and structure of human brain creatine kinase.
Acrylamide is widely used worldwide in industry and it can also be produced by the cooking and processing of foods. It is harmful to human beings, and human brain CK (HBCK) has been proposed to be one of the important targets of acrylamide. In this research, we studied the effects of acrylamide on HBCK activity, structure and the potential binding sites. Compared to CKs from rabbit, HBCK was fully inactivated at several-fold lower concentrations of acrylamide, and exhibited distinct properties upon acrylamide-induced inactivation and structural changes. The binding sites of acrylamide were located at the cleft between the N- and C-terminal domains of CK, and Glu232 was one of the key binding residues. The effects of acrylamide on CK were proposed to be isoenzyme- and species-specific, and the underlying molecular mechanisms were discussed.
Sheng
Qing
Q
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
Zou
He-Chang
HC
Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314050, China.|State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Lü
Zhi-Rong
ZR
Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Zou
Fei
F
Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Park
Yong-Doo
YD
Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314050, China.|Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Yan
Yong-Bin
YB
State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Yao
Shan-Jing
SJ
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
D020106:Acrylamide / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D052276:Creatine Kinase, BB Form / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D062105:Molecular Docking Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011817:Rabbits
D020106:Acrylamide; D052276:Creatine Kinase, BB Form