N-Glycosylation profiling of turtle egg yolk: expression of galabiose structure.
To understand the roles of species-specific carbohydrates, systematic studies of interspecific glycan analyses are imperative. An extensive series of glycomics studies on approximately 180 kinds of bird eggs have demonstrated that 60-70% of the birds, which are closely related in phylogeny, express the alpha-Galp-(1-->4)-Galp structure on their egg glycoproteins. This prompted us to investigate the glycosylation profiles of eggs from an evolutionarily related organism, a sea turtle (reptilian). We performed N-glycosylation profiling of turtle egg yolk by using HPLC mapping in conjunction with mass spectrometric methods and thereby demonstrated that the alpha-Galp-(1-->4)-Galp groups are displayed on approximately 38% of total N-glycans. Our findings suggest that the ability to express the galabiose structure was acquired at an early stage of diversification in amniotes.
Yagi
Hirokazu
H
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
Yamamoto
Masahiro
M
Yu
Shin-Yi
SY
Takahashi
Noriko
N
Khoo
Kay-Hooi
KH
Lee
Yuan Chuan
YC
Kato
Koichi
K
D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis* / Q000096:biosynthesis; D004530:Egg Yolk / Q000737:chemistry*; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014426:Turtles / Q000378:metabolism*
D004187:Disaccharides; C032103:4-O-alpha-D-galactopyranosyl-D-galactose