title: Bone marrow cells obtained from cirrhotic rats do not improve function or reduce fibrosis in a chronic liver disease model.
abstract: BACKGROUND
  The objective of this study was to evaluate the therapeutic potential of bone marrow cells (BMCs) obtained from cirrhotic donors in a model of chronic liver disease.
  
  
  METHODS
  Chronic liver injury was induced in female Wistar rats by the association of an alcoholic diet with intraperitoneal injections of carbon tetrachloride. BMCs obtained from cirrhotic donors or placebo were injected through the portal vein. Blood analysis of alanine aminotransferase (ALT) and albumin levels, ultrasound assessment including the measurement of the portal vein diameter (PVD) and liver echogenicity, histologic evaluation with hematoxylin and eosin and Sirius red staining, and quantification of collagen deposition were performed.
  
  
  RESULTS
  ALT and albumin blood levels showed no significant differences between the experimental groups two months after injection. Additionally, no significant variation in PVD and liver echogenicity was found. Histological analysis also showed no significant variation in collagen deposition two months after placebo or BMC injection.
  
  
  CONCLUSIONS
  This study suggests that, even though BMC therapy using cells from healthy donors has previously shown to be effective, this is not the case when BMCs are obtained from cirrhotic animals. This result has major clinical implications when considering the use of autologous BMCs from patients with chronic liver diseases.
authors.affiliation: Centro de Cirurgia Experimental e Programa de Pós-Graduação, Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Elida Gripp
authors.initials: EG
authors.lastname: Mannheimer
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Quintanilha
authors.forename: Adriana Bastos
authors.initials: AB
authors.lastname: Carvalho
authors.forename: Bruno Diaz
authors.initials: BD
authors.lastname: Paredes
authors.forename: Felipe
authors.initials: F
authors.lastname: Gonçalves de Carvalho
authors.forename: Cristina Maeda
authors.initials: CM
authors.lastname: Takyia
authors.forename: Célia Maria Coelho
authors.initials: CM
authors.lastname: Resende
authors.forename: Guilherme
authors.initials: G
authors.lastname: Ferreira da Motta Rezende
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Campos de Carvalho
authors.forename: Alberto
authors.initials: A
authors.lastname: Schanaider
authors.forename: Regina Coeli
authors.initials: RC
authors.lastname: dos Santos Goldenberg
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride / Q000633:toxicity; D004195:Disease Models, Animal; D058625:End Stage Liver Disease / Q000150:complications* / Q000628:therapy*; D005260:Female; D008103:Liver Cirrhosis / Q000209:etiology* / Q000473:pathology*; D008111:Liver Function Tests; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002251:Carbon Tetrachloride