title: WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana.
abstract: Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild-type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6-3 and the wild-type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. Transcriptome analysis around the root tip identified WRKY6-regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency.
authors.affiliation: Biotechnology Research Center, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. kasajima2008@live.jp
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kasajima
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ide
authors.forename: Masami
authors.initials: M
authors.lastname: Yokota Hirai
authors.forename: Toru
authors.initials: T
authors.lastname: Fujiwara
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D001895:Boron / Q000172:deficiency*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors; C446285:WRKY6 protein, Arabidopsis; D001895:Boron