Inhibitory effect of Angelicae Tenuissimae Radix on expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells.
OBJECTIVE
Angelicae Tenuissimae Radix has traditionally been used for treating headache, flu-like symptoms, limb-ache and disability, and even for treating toothache. We investigated the anti-inflammatory and analgesic effect of Angelicae Tenuissimae Radix on lipopolysaccharide-induced inflammation.


METHODS
For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2) immunoassay and nitric oxide detection in mouse BV2 microglial cells were performed.


RESULTS
Angelicae Tenuissimae Radix suppressed prostaglandin E(2) synthesis and nitric oxide production by inhibiting the lipopolysaccharide-induced expressions of cyclooxygenase-2 and inducible nitric oxide synthase messenger RNA in mouse BV2 microglial cells.


CONCLUSIONS
The present study indicates that Angelicae Tenuissimae Radix can be applied as a valuable treatment for brain inflammation and headache.
Lee
Suh-Ha
SH
Department of Herbology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea.
Choi
Hoyoung
H
Kim
Hocheol
H
Lee
Hyejung
H
Sung
Yun-Hee
YH
Kim
Sung-Eun
SE
Chang
Hyun-Kyung
HK
Shin
Min-Chul
MC
Shin
Mal-Soon
MS
Kim
Chang-Ju
CJ
D029969:Angelica; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007118:Immunoassay; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C022616:thiazolyl blue