title: [Determination of LC 90 and LT 90 of IBCB66 Beauveria bassiana (Ascomycetes: Clavicipitaceae) isolate for Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) control].
abstract: The objective of this research was to evaluate the pathogenicity and the virulence of the IBCB66 isolate of Beauveria bassiana on infected larvae of Rhipicephalus (Boophilus) microplus. The IBCB66 fungus strain was used as standard isolates of B. bassiana against R. (B.) microplus larvae. The larval bioassay tests using the IBCB66 isolate were carried out to determine the (Lethal Concentration) LC50, LC90, (Lethal Time) LT50 and LT90. The IBCB66 fungus strain was tested at six different concentrations (5x10(6), 10(7), 5x10(7), 10(8), 5x10(8) and 10(9) conidia.mL(-1)) to determine the percentage of larval mortality. In addition, a Probit analysis was also performed. Total larval mortality was observed eighteen days after the beginning of the test in the group treated with 5x10(9) conidia.mL(1). The LC50 and LC90 were 3x10(7) and 5x10(8) respectively and the LT50 and LT90 were 10 and 16 days.
authors.affiliation: Centro de Pesquisa e Desenvolvimento de Sanidade Animal-CPDSA, Instituto Biológico, Av. Conselheiro Rodrigues Alves 1.252, CEP 04.014-002, São Paulo-SP, Brazil. barci@biologico.sp.gov.br
authors.forename: Leila A G
authors.initials: LA
authors.lastname: Barci
authors.forename: José Eduardo M
authors.initials: JE
authors.lastname: de Almeida
authors.forename: Adriana H
authors.initials: AH
authors.lastname: de Campos Nogueira
authors.forename: Angelo P
authors.initials: AP
authors.lastname: do Prado
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000302:isolation & purification / Q000472:pathogenicity*; D052982:Beauveria / Q000302:isolation & purification / Q000472:pathogenicity*; D026863:Ixodidae; D010572:Pest Control, Biological / Q000379:methods