Simplified DGS procedure for large-scale genome structural study.
Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the original protocol by replacing the in vivo genomic DNA cloning with in vitro adaptor ligation, eliminating the ditag concatemerization steps, and replacing the 454 sequencer with Solexa or SOLiD sequencers for ditag sequence collection. This revised protocol further increases genome coverage and resolution and allows DGS to be used to analyze multiple genomes simultaneously.
Jung
Yong-Chul
YC
Center for Functional Genomics, ENH Research Institute, Northwestern University, 1001 University Place, Evanston, IL 60201, USA.
Xu
Jia
J
Chen
Jun
J
Kim
Yeong
Y
Winchester
David
D
Wang
San Ming
SM
D000465:Algorithms; D002874:Chromosome Mapping / Q000379:methods*; D020224:Expressed Sequence Tags; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*