Effects of capture stress on free-ranging, reproductively active male Weddell seals.
Physiological stress responses to capture may be an indicator of welfare challenges induced by animal handling. Simultaneously, blood chemistry changes induced by stress responses may confound experimental design by interacting with the biological parameters being measured. Cortisol elevation is a common indicator of stress responses in mammals and reproductive condition can profoundly influence endocrine response. We measured changes in blood cortisol and testosterone induced by handling reproductively active male Weddell seals (Leptonychotes weddellii) early and late in the breeding season. Weddell seals have the highest resting cortisol levels of all mammals yet showed a clear, prolonged elevation in cortisol in response to capture. Responses were similar when first caught and when caught a second time, later in the breeding season. Baseline testosterone levels declined over the breeding season but were not altered by capture. Administering a light dose of diazepam significantly ameliorated the cortisol response of handled animals without affecting testosterone levels. This may be an effective way of reducing acute capture stress responses. Male breeding success in years males were handled was no different to the years they were not, despite the acute capture response, suggesting no long-term impact of handling on male reproductive output.
Harcourt
Robert Geoffrey
RG
Marine Mammal Research Group, Graduate School of the Environment, Macquarie University, Sydney, NSW, 2109, Australia. rharcour@gse.mq.edu.au
Turner
Emma
E
Hall
Ailsa
A
Waas
Joseph R
JR
Hindell
Mark
M
D000704:Analysis of Variance; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D012098:Reproduction / Q000187:drug effects / Q000502:physiology*; D012149:Restraint, Physical / Q000379:methods / Q000523:psychology; D046048:Seals, Earless / Q000502:physiology*; D012621:Seasons; D013315:Stress, Psychological / Q000097:blood / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood
D014151:Anti-Anxiety Agents; D013739:Testosterone; D003975:Diazepam; D006854:Hydrocortisone