title: The challenges of the analysis of basic compounds by high performance liquid chromatography: some possible approaches for improved separations.
abstract: This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed. Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds.
authors.affiliation: Centre for Research in Biomedicine, University of the West of England, Frenchay, Bristol BS16 1QY, UK. david.mccalley@uwe.ac.uk
authors.forename: David V
authors.initials: DV
authors.lastname: McCalley
mesh_terms: D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature
chemical_list: D000838:Anions; D002412:Cations; D012822:Silicon Dioxide