title: Hippocampal neurovascular and hypothalamic-pituitary-adrenal axis alterations in spontaneously type 2 diabetic GK rats.
abstract: Metabolic and vascular consequences of diabetes mellitus induce several CNS complications. The dentate gyrus of the hippocampus, a well-recognized target for diabetic alterations, is a neurogenic area associated with memory and learning processes. Here, we explored the hippocampal neurogenesis and its microenvironment (astrocytes, vascularisation and glucocorticoid influence) in a spontaneous model of type 2 diabetes, the Goto-Kakizaki rat. The number of proliferative Ki67(+) cells and young doublecortin(+) neurons was 2-fold higher in the hippocampus from diabetic rats than in normoglycemic control Wistar at 4 months of age. However, there was no difference in cell survival, studied 3 weeks after bromodeoxyuridine administration. Labeling of endothelial cells against von Willebrand factor, demonstrated a 50% decrease in the granular cell layer fractional area covered by blood vessels and a diminished capillary branching in diabetic rats. Finally, Goto-Kakizaki rats exhibited decreased glucocorticoid receptor immunolabeling in CA1, associated with higher corticosteronemia. In conclusion, diabetic rats showed increased cell proliferation and neuronal differentiation without concomitant survival modification. A high proliferation rate, potentially reflecting a compensatory mechanism for neuronal suffering, also exists in various pathological situations. However, endothelial alteration induced by chronic hyperglycemia, hyperleptinemia and insulin resistance and associated with deleterious glucocorticoid effects might impair effective neurogenesis in diabetic Goto-Kakizaki rats.
authors.affiliation: Institute of Biology and Experimental Medicine, CONICET National Research Council, Buenos Aires, Argentina.
authors.forename: Juan
authors.initials: J
authors.lastname: Beauquis
authors.forename: Françoise
authors.initials: F
authors.lastname: Homo-Delarche
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Giroix
authors.forename: Jan
authors.initials: J
authors.lastname: Ehses
authors.forename: Josiane
authors.initials: J
authors.lastname: Coulaud
authors.forename: Paulina
authors.initials: P
authors.lastname: Roig
authors.forename: Bernard
authors.initials: B
authors.lastname: Portha
authors.forename: Alejandro F
authors.initials: AF
authors.lastname: De Nicola
authors.forename: Flavia
authors.initials: F
authors.lastname: Saravia
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D004195:Disease Models, Animal; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D042783:Endothelial Cells / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000503:physiopathology*; D007328:Insulin / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D001786:Blood Glucose; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005904:Glial Fibrillary Acidic Protein; D007328:Insulin; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D011965:Receptors, Glucocorticoid; D014841:von Willebrand Factor; D001973:Bromodeoxyuridine