Evaluation of various ions and compounds on nitrilase produced from Streptomyces sp.
The nitrilase produced from a new isolate is evaluated for its activity in presence of a number of different ions and compounds at optimal conditions. It was found that the activity of nitrilase increased up to 10-20% in presence of most of the divalent ions at a concentration of 5 mM relative to the control. Silver, mercury, tin, DTT, ascorbic acid and thiourea, respectively, were observed as potential inhibitors of the enzyme catalysis. The investigation on storage stability of whole cells in presence of a number of stabilizers showed that the enzyme is stable (relative activity 50%) for more than 120 days at various temperatures.
Khandelwal
Amit Kumar
AK
Birla Institute of Scientific Research, Jaipur, Rajasthan, India.
Nigam
Vinod Kumar
VK
Vidyarthi
Ambarish S
AS
Ghosh
Purnendu
P
D000619:Aminohydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002413:Cations, Divalent / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D007218:Industrial Microbiology; D008670:Metals / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D009570:Nitriles / Q000378:metabolism*; D055550:Protein Stability / Q000187:drug effects; D012498:Sanitary Engineering / Q000379:methods; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
D002413:Cations, Divalent; D008670:Metals; D009570:Nitriles; D000619:Aminohydrolases; C021867:nitrilase