title: A potential role for Helicobacter pylori heat shock protein 60 in gastric tumorigenesis.
abstract: Helicobacter pylori has been found to promote the malignant process leading to gastric cancer. Heat shock protein 60 of H. pylori (HpHSP60) was previously been identified as a potent immunogene. This study investigates the role of HpHSP60 in gastric cancer carcinogenesis. The effect of HpHSP60 on cell proliferation, anti-death activity, angiogenesis and cell migration were explored. The results showed that HpHSP60 enhanced migration by gastric cancer cells and promoted tube formation by umbilical vein endothelial cells (HUVECs); however, HpHSP60 did not increase cell proliferation nor was this protein able to rescue gastric cancer cells from death. Moreover, the results also indicated HpHSP60 had different effects on AGS gastric cancer cells or THP-1 monocytic cells in terms of their expression of pro-inflammatory cytokines, which are known to be important to cancer development. We propose that HpHSP60 may trigger the initiation of carcinogenesis by inducing pro-inflammatory cytokine release and by promoting angiogenesis and metastasis. Thus, this extracellular pathogen-derived HSP60 is potentially a vigorous virulence factor that can act as a carcinogen during gastric tumorigenesis.
authors.affiliation: Department of Biological Science and Technology, National Chiao-Tung University, Hsin-Chu, Taiwan.
authors.forename: Chen-Si
authors.initials: CS
authors.lastname: Lin
authors.forename: Pei-Juin
authors.initials: PJ
authors.lastname: He
authors.forename: Nu-Man
authors.initials: NM
authors.lastname: Tsai
authors.forename: Chi-Han
authors.initials: CH
authors.lastname: Li
authors.forename: Shang-Chih
authors.initials: SC
authors.lastname: Yang
authors.forename: Wei-Tung
authors.initials: WT
authors.lastname: Hsu
authors.forename: Ming-Shiang
authors.initials: MS
authors.lastname: Wu
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
authors.forename: Tain-Lu
authors.initials: TL
authors.lastname: Cheng
authors.forename: Kuang-Wen
authors.initials: KW
authors.lastname: Liao
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D016207:Cytokines / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000139:chemically induced / Q000382:microbiology; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000382:microbiology*
chemical_list: C078096:60K protein, Helicobacter pylori; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines