title: Estrogen and inflammation modulate estrogen receptor alpha expression in specific tissues of the temporomandibular joint.
abstract: BACKGROUND
  Estrogen is known to play role in temporomandibular joint (TMJ) disorders and estrogen effects can be mediated by estrogen receptor (ER) alpha present in the TMJ. Cells expressing the estrogen receptor ERalpha are present in the temporomandibular joint (TMJ) but changes in expression due to estrogen and inflammation have not been characterized. In this study, ERalpha protein content and the number of cells expressing ERalpha was measured in 17 beta-estradiol-treated rats after inflammation was induced in the TMJ.
  
  
  METHODS
  Sixteen ovariectomized female rats were divided into two groups such that one group received 17 beta estradiol (E2) and the other was given vehicle (VEH). Groups were then subdivided further, one received injections of saline and the other received Complete Freund's adjuvant (CFA) within the superior joint space of the TMJ. Thus the four groups include no E2/saline, E2/saline, no E2/CFA and E2/CFA. After treatment, the rats were sacrificed, and the TMJ anterior, disc, retrodiscal and synovial tissues were analyzed by western blot and immunocytochemistry. Positive stained cells were counted using a Nikon epifluorescent microscope.
  
  
  RESULTS
  The western blot showed that ERalpha protein significantly decreased with inflammation. The number of ERalpha-positive cells in the TMJ was not affected by inflammation or 17 beta-estradiol with exception of the retrodiscal tissue. In the retrodiscal tissue 17 beta-estradiol significantly decreased the number of ERalpha-positive cells but only in a non-inflamed joint.
  
  
  CONCLUSIONS
  In conclusion, inflammation and 17 beta-estradiol can modulate ERalpha expression in the TMJ but the effects are tissue specific.
authors.affiliation: Department of Biomedical Sciences, Texas A&M Health Science Center, Baylor College of Dentistry, Dallas, Texas, USA. jpuri@bcd.tamhsc.edu
authors.forename: Jyoti
authors.initials: J
authors.lastname: Puri
authors.forename: Bob
authors.initials: B
authors.lastname: Hutchins
authors.forename: Larry L
authors.initials: LL
authors.lastname: Bellinger
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Kramer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000502:physiology*; D004967:Estrogens / Q000502:physiology*; D005260:Female; D005620:Freund's Adjuvant; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000473:pathology; D013704:Temporomandibular Joint / Q000378:metabolism* / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology
chemical_list: D047628:Estrogen Receptor alpha; D004967:Estrogens; D004958:Estradiol; D005620:Freund's Adjuvant