title: [Preparation and preliminary evaluation of KGDS-targeted ultrasound contrast agent].
abstract: OBJECTIVE
  To prepare a thrombus-targeted ultrasonic contrast agent and to investigate its targeted ability to fresh blood clots.
  
  
  METHODS
  We first synthesized FITC-KGDS-Palm compound, and then prepared thrombus-targeted microbubbles using "ultrasound & high speed shearing method". Fluorescence labeling thrombus-specific peptides and KGDS, directed at the activated glycoprotein(GP)IIb/IIIa receptor of platelets were attached to the surface of lipid microbubbles. The concentration and size of TUCA were measured by Malvern Zeta Sizer Nano-ZS590 and Coulter counter. Immunofluorescence was applied to confirm the conjugation. The conjunct ratio was assessed by flow cytometer (FCM).
  
  
  RESULTS
  The KGDS-TUCA was straw yellow turbid liquor, and the concentration was 1.5 x 10(9)/mL, and the average size was 1.5 microm. The targeted microbubbles conjugated with the thrombus-specific peptides showed bright green rings by fluorescence microscope. FCM demonstrated that the wavelength of shell of KGDS-TUCA changed greatly, and the conjunct ratio was 90.04%. In vitro study showed KGDS-TUCA remained stable for 48 h at 4 degree C and target-attached to blood clots and showed good stability.
  
  
  CONCLUSIONS
  The ultrasound & high speed shearing method to prepare TUCA is easy and in favor of purification. KGDS-TUCA has high specific biological activity. The conjunct ratio and stability of KGDS-TUCA are excellent.
authors.affiliation: Institute of Cell Transplantation & Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Ding
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Sheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.forename: Zhuoqiong
authors.initials: Z
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000737:chemistry; D003287:Contrast Media / Q000138:chemical synthesis*; D006801:Humans; D045423:Microbubbles; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes; D010455:Peptides / Q000737:chemistry; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D012680:Sensitivity and Specificity; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014463:Ultrasonography
chemical_list: D000596:Amino Acids; D003287:Contrast Media; D015335:Molecular Probes; D010455:Peptides; D019039:Platelet Glycoprotein GPIIb-IIIa Complex