Modulation of p53 gene expression in the intertidal copepod Tigriopus japonicus exposed to alkylphenols.
The copepod, Tigriopus japonicus has been recognized as an excellent marine model species for ecotoxicological studies. The present study cloned and characterized the p53 gene of this copepod and studied its expression pattern. We discovered that p53 expression patterns varied among different developmental stages of the copepod, having the highest expression in the adult. Such variation was possibly associated with the molting cycle. By using real-time RT-PCR, we further investigated the modulatory pattern of the p53 gene in the copepod after exposure to three alkylphenols (i.e. nonylphenol, octylphenol, and bisphenol A) which are known as endocrine disruption chemicals (EDCs). The results showed that the three alkylphenols significantly induced p53 gene expression in the copepod, indicating the involvement of p53 in such stress-responses. Thus, the copepod p53 gene provides one of the stress-response biomarkers for exposure to EDC-like compounds.
Hwang
Dae-Sik
DS
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, South Korea.
Lee
Jin-Seon
JS
Rhee
Jae-Sung
JS
Han
Jeonghoon
J
Lee
Young-Mi
YM
Kim
Il-Chan
IC
Park
Gyung Soo
GS
Lee
Jehee
J
Lee
Jae-Seong
JS
D000818:Animals; D001559:Benzhydryl Compounds; D015415:Biomarkers / Q000378:metabolism; D033342:Copepoda / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D016158:Genes, p53 / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D015415:Biomarkers; D010636:Phenols; D016159:Tumor Suppressor Protein p53; D014874:Water Pollutants, Chemical; C474055:octylphenol; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds