[Expression of osteopontin and osteocalcin during distraction osteogenesis on rhesus with cleft palate].
OBJECTIVE
To study the mechanism of new bone formation and remodeling of distraction osteogenesis (DO) by analysis of the expression of osteopontin (OPN) and osteocalcin (OC).


METHODS
Rhesus were operated to reconstruct the animal model of cleft palate (CP). The CP was closed by DO in experimental group(n = 21). After consolidation of 1, 2, 4, 6, 8, 12, 24 weeks, every 3 animals were killed to collect the specimens, respectively. The OPN and OC and their mRNA were detected quantitatively by Real-time RT-PCR and ELISA, respectively. The animals in control group (n = 2) and sham group (n = 2) were used as control.


RESULTS
The mRNA expression of OPN increased since 2nd week of consolidation and reached the peak at 4th week (7.59 +/- 0.37). The mRNA expression of OC was up-regulated since 4th week, and reach the peak at 6th week (7.94 +/- 0.31). Then they decreased to about the level in sham group at 24th week (P > 0.05). The OPN and OC were highly expressed during 4 to 6 weeks of consolidation. During 8 to 12 weeks, they decreased like their mRNA expression.


CONCLUSIONS
The intramembraneous new bone formation after DO can reconstruct the bone defect of CP. The new formed bone can be remodeled to be quite normal bone tissue.
Liu
Yan-Shan
YS
Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
Chen
Gang
G
Liu
Yi
Y
Li
Rui
R
Wang
Zhi-Qi
ZQ
Shen
Dai
D
D000818:Animals; D002972:Cleft Palate / Q000378:metabolism* / Q000601:surgery*; D008253:Macaca mulatta; D015675:Osteocalcin / Q000378:metabolism*; D019857:Osteogenesis, Distraction; D053495:Osteopontin / Q000378:metabolism*
D015675:Osteocalcin; D053495:Osteopontin