title: On-line desorption of dried blood spots coupled to hydrophilic interaction/reversed-phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites.
abstract: An assay for the simultaneous analysis of pharmaceutical compounds and their metabolites from micro-whole blood samples (i.e. 5 microL) was developed using an on-line dried blood spot (on-line DBS) device coupled with hydrophilic interaction/reversed-phase (HILIC/RP) LC/MS/MS. Filter paper is directly integrated to the LC device using a homemade inox desorption cell. Without any sample pretreatment, analytes are desorbed from the paper towards an automated system of valves linking a zwitterionic-HILIC column to an RP C18 column. In the same run, the polar fraction is separated by the zwitterionic-HILIC column while the non-polar fraction is eluted on the RP C18. Both fractions are detected by IT-MS operating in full scan mode for the survey scan and in product ion mode for the dependant scan using an ESI source. The procedure was evaluated by the simultaneous qualitative analysis of four probes and their relative phase I and II metabolites spiked in whole blood. In addition, the method was successfully applied to the in vivo monitoring of buprenorphine metabolism after the administration of an intraperitoneal injection of 30 mg/kg on adult female Wistar rat.
authors.affiliation: Unit of Toxicology, University Center of Legal Medicine, Geneva, Switzerland. aurelien.thomas@hcuge.ch
authors.forename: Aurélien
authors.initials: A
authors.lastname: Thomas
authors.forename: Julien
authors.initials: J
authors.lastname: Déglon
authors.forename: Thierry
authors.initials: T
authors.lastname: Steimer
authors.forename: Patrice
authors.initials: P
authors.lastname: Mangin
authors.forename: Youssef
authors.initials: Y
authors.lastname: Daali
authors.forename: Christian
authors.initials: C
authors.lastname: Staub
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations