title: [Advances in the study of affinity selection-ultrafiltration/HPLC-MS].
abstract: Affinity selection-ultrafiltration/HPLC-MS is the combination of the ultrafiltration and HPLC-MS, mainly used for screening small active molecular substances from combinatorial libraries and natural product extracts, which can bind to solution-phase targets. Besides, it can be used in metabolic screening and characterization of ligand-receptor binding. It is a complement to the traditional methods of screening and identifying drugs. This review describes its principle and application in drug study.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhao
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Xiao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding; D054852:Small Molecule Libraries; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D008024:Ligands; D004364:Pharmaceutical Preparations; D054852:Small Molecule Libraries