title: Osteogenic differentiation capacity of porcine dental follicle progenitor cells.
abstract: This study examined the effect of extracellular matrix (ECM) on the osteogenic differentiation capacity and osteogenesis of dental follicle cells. Single cell-derived porcine dental follicle cells (DFC-I) obtained at the early stage of crown formation in tooth were subcultured and characterized using periodontal ligament cells (PDLC) and bone marrow-derived mesenchymal stem cells (BMSC) as comparison cell populations. The effect of ECM constituents including collagen type I, fibronectin, laminin, and collagen type IV on the differentiation of DFC-1 into osteogenic-lineage cells was evaluated in vitro. In addition, the DFC-1, PDLC, and BMSC populations were compared for osteogenic capacity in vitro by Alizarin red staining and in vivo by transplantation. DFC-I showed different features from PDLC and BMSC. Different components of ECM had different effects on the differentiation of DFC-1 into osteogenic-lineage cells in vitro. Alkaline phosphatase activity and matrix mineralization as early- and late-stage markers of osteogenesis, respectively, supported the differentiation of DFC-1 into osteogenic-related cells in vitro. All three cell types showed equivalent osteogenic capacity in vivo at 4 weeks postoperatively. There were no statistically significant differences among the cell populations with respect to capacity for bone formation. These results suggest a potential application for dental follicle cells in bone-tissue engineering.
authors.affiliation: Department of Anatomy, Nihon University School of Dentistry, Division of Stem Cell Engineering, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohshima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yamakoshi
authors.forename: James P
authors.initials: JP
authors.lastname: Simmer
authors.forename: Masaki J
authors.initials: MJ
authors.lastname: Honda
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D003795:Dental Sac / Q000166:cytology* / Q000187:drug effects; D005109:Extracellular Matrix / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology; D013234:Stem Cells; D013552:Swine; D023822:Tissue Engineering
chemical_list: D016326:Extracellular Matrix Proteins