[Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen].
OBJECTIVE
To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).


METHODS
The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.


RESULTS
The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).


CONCLUSIONS
Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.
Qiu
Cheng-feng
CF
Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. qiuchengfeng0721@yahoo.com.cn
Lei
Lin-sheng
LS
Wu
Yu-ying
YY
Yu
Chuan-lin
CL
Zhu
Zheng-guang
ZG
Chen
Na-na
NN
Wu
Shu-guang
SG
D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000097:blood; D056724:Immunity, Humoral; D007114:Immunization; D008297:Male; D051379:Mice / Q000276:immunology*; D023421:Models, Animal; D011817:Rabbits
D006460:Hemolysin Proteins