Growth of Pseudomonas sp. TX1 on a wide range of octylphenol polyethoxylate concentrations and the formation of dicarboxylated metabolites.
Pseudomonas sp. TX1, is able to use octylphenol polyethoxylates (OPEO(n), or Triton X-100; average n = 9.5) as a sole carbon source. It can grow on 0.05-20% of OPEO(n) with a specific growth rate of 0.34-0.44 h(-1). High-performance liquid chromatography-mass spectrometer analysis of OPEO(n) degraded metabolites revealed that strain TX1 was able to shorten the ethoxylate chain and produce octylphenol (OP). Furthermore, formation of the short carboxylate metabolites, such as carboxyoctylphenol polyethoxylates (COPEO(n), n = 2, 3) and carboxyoctylphenol polyethoxycarboxylates (COPEC(n), n = 2, 3) began at the log stage, while octylphenol polyethoxycarboxylates (OPEC(n), n = 1-3) was formed at the stationary phase. All the short-ethoxylated metabolites, OPEO(n), OPEC(n), COPEO(n), and COPEC(n), accumulated when the cells were in the stationary phase. This study is the first to demonstrate the formation of COPEO(n) and COPEC(n) from OPEO(n) by an aerobic bacterium.
Lin
Yi-Wen
YW
Department of Life Sciences, National Central University, No. 300 Chung-da Rd., Chung-li, 32001, Taiwan, ROC.
Guo
Gia-Luen
GL
Hsieh
Hsiao-Cheng
HC
Huang
Shir-Ly
SL
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003998:Dicarboxylic Acids / Q000378:metabolism*; D013058:Mass Spectrometry; D017830:Octoxynol / Q000378:metabolism*; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D003998:Dicarboxylic Acids; D014874:Water Pollutants, Chemical; D017830:Octoxynol