Panel reactive HLA antibodies, soluble CD30 levels, and acute rejection six months following renal transplant.
BACKGROUND
Specific anti-human leukocyte antigen antibodies (HLA) in the post-transplant period may be present with acute rejection episodes (ARE), and high soluble CD30 (sCD30) serum levels may be a risk factor for ARE and graft loss.


METHODS
HLA cross-matching, panel reactive antibodies (PRA), and sCD30 levels were determined prior to transplantation in 72 patients. Soluble CD30 levels and PRA were re-assessed at day 7, 14, 21, and 28, and monthly up to the sixth.


RESULTS
Twenty-four subjects had a positive PRA and 17 experienced ARE. Nine of 17 ARE subjects demonstrated positive PRA and 16 had HLA mismatches. Positive PRA was more frequent in ARE subjects (p = 0.03). Eight subjects with ARE had donor-specific antibodies (DSA) in serum samples pre-transplantation, two subjects developed DSA. Three subjects without ARE had positive PRA only in post-transplantation samples. Soluble CD30 levels were higher in pre-transplant samples and ARE subjects than non-ARE subjects (p = 0.03). Post-transplant sCD30 levels were elevated in subjects who experienced rejection and were significantly higher at seven d (p = 0.0004) and six months (p = 0.03).


CONCLUSIONS
Higher sCD30 levels following transplant were associated with ARE. Elevated sCD30 levels may represent a risk factor for acute rejection.
Domingues
Elizabeth M F L
EM
Histocompatibility and Cryopreservation Laboratory, Rio de Janeiro State University Nephrology Service, Bonsucesso General Hospital, Rio de Janeiro, RJ, Brazil.
Matuck
Teresa
T
Graciano
Miguel L
ML
Souza
Edison
E
Rioja
Suzimar
S
Falci
Mônica C
MC
Monteiro de Carvalho
Deise B
DB
Porto
Luís Cristóvão
LC
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D017730:Ki-1 Antigen / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D012307:Risk Factors; D015996:Survival Rate
D001323:Autoantibodies; D006680:HLA Antigens; D007518:Isoantibodies; D017730:Ki-1 Antigen