Relationship between glucose catabolism and xanthan production in Xanthomonas oryzae pv. oryzae.
Two genes involved in central carbon metabolism were inactivated to modulate intracellular glucose 6-phosphate and to evaluate its effects on xanthan production in wild-type Xanthomonas oryzae pv. oryzae. Upon the inactivation of the phosphogluconate dehydratase gene (edd), intracellular glucose 6-phosphate increased from 0.05 to 1.17 mmol/g (dry cell wt). This was accompanied by increased xanthan production of up to 2.55 g/l (culture medium). In contrast, inactivation of 6-phosphogluconate dehydrogenase gene (gndA) did not influence intracellular glucose 6-phosphate nor xanthan production. The intracellular availability of glucose 6-phosphate is proposed as a rate-limiting factor in xanthan production, and it may be possible to increases production of xanthan by modulating the activities of enzymes in central carbon metabolism.
Kim
Sang-Yoon
SY
Division of Animal Science and Biotechnology, Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do 220-702, Korea.
Lee
Byoung-Moo
BM
Cho
Jae-Yong
JY
D002244:Carbon / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D009154:Mutation; D010733:Phosphoglucomutase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D015398:Signal Transduction; D014974:Xanthomonas / Q000235:genetics / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; D019298:Glucose-6-Phosphate; D002244:Carbon; D010734:Phosphogluconate Dehydrogenase; D010733:Phosphoglucomutase; D005947:Glucose; C002563:xanthan gum