Protective effect of resveratrol on formation of membrane protein carbonyls and lipid peroxidation in erythrocytes subjected to oxidative stress.
Many of the biological actions of resveratrol have been attributed to its antioxidant properties. In this work, we subjected human erythrocytes to in vitro oxidative stress by incubating them with tert-butylhydroperoxide (t-BHP). This caused a significant increase in the malondialdehyde (MDA) level and the protein carbonyl group content above the basal values. The presence of trans-resveratrol at micromolar concentrations in the incubation medium protected the erythrocytes from t-BHP-induced oxidative stress, as evidenced by the decrease in the MDA level and the protein carbonyl group content. The effect of resveratrol was concentration and time-dependent. Our findings help to explain some of the beneficial effects of resveratrol.
Pandey
Kanti Bhooshan
KB
Department of Biochemistry, University of Allahabad, Allahabad, 211002 India.
Rizvi
Syed Ibrahim
SI
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors; D055815:Young Adult; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000975:Antioxidants; D015415:Biomarkers; D008565:Membrane Proteins; D013267:Stilbenes; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D000077185:Resveratrol