A proteome map of the pituitary melanotrope cell activated by black-background adaptation of Xenopus laevis.
Upon transfer of Xenopus laevis from a white to a black background, the melanotrope cells in the pituitary pars intermedia secrete alpha-melanocyte-stimulating hormone, which stimulates dispersion of melanin pigment in skin melanophores. This adaptive behavior is under the control of neurotransmitters and neuropeptides of hypothalamic origin. The alpha-melanocyte-stimulating hormone-producing cells and their hypothalamic control system provide an interesting model to study proteins required for biosynthetic and secretory processes involved in peptide hormone production and for brain-pituitary signaling. We present a 2-D PAGE-based proteome map of melanotrope cells from black-adapted animals, identifying 204 different proteins by MS analysis.
Devreese
Bart
B
Laboratory for Protein Biochemistry and Biomolecular Engineering, Ghent University, Gent, Belgium. Bart.devreese@ugent.be
Sergeant
Kjell
K
Van Bakel
Nick H M
NH
Debyser
Griet
G
Van Beeumen
Jozef
J
Martens
Gerard J M
GJ
Van Herp
François
F
D000064:Acclimatization; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D052717:Melanotrophs / Q000378:metabolism*; D010449:Peptide Mapping; D010902:Pituitary Gland / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
D020543:Proteome