title: A new multiple-drug applicator with minimal drug cross-talk, leakage, and consumption.
abstract: The relative effects of multiple drugs give an important clue to dissect a neuronal mechanism and to seek for a candidate neurotherapeutical agent. Here we have devised a "flute" applicator which can deliver several drugs to a neural cell preparation. The applicator stands by, cleaning itself with bath perfusate and delivers drugs only during test applications. This minimizes drug cross-talk in and leakage from the applicator and drug consumption. Using the applicator, we successfully compared the relative effects of widely different doses of an agonist in single neurons. The flute applicator would be a useful tool for pharmacological analyses.
authors.affiliation: Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Fujita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimomura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Hosoguchi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kano
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Fukurotani
authors.forename: Toshihide
authors.initials: T
authors.lastname: Tabata
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004801:Eosine Yellowish-(YS) / Q000008:administration & dosage; D004868:Equipment Failure; D005456:Fluorescent Dyes / Q000008:administration & dosage; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000008:administration & dosage; D051379:Mice; D018408:Patch-Clamp Techniques; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D010600:Pharmacology; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists; D012118:Resorcinols / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D018094:Receptors, Metabotropic Glutamate; D012118:Resorcinols; C104077:metabotropic glutamate receptor type 1; C079215:3,5-dihydroxyphenylglycine; C017475:lucifer yellow; D004801:Eosine Yellowish-(YS); D005998:Glycine