Crystallization and preliminary crystallographic analysis of the measles virus hemagglutinin in complex with the CD46 receptor.
The measles virus (MV) hemagglutinin (MV-H) mediates the attachment of MV particles to cell-surface receptors for entry into host cells. MV uses two receptors for attachment to host cells: the complement-control protein CD46 and the signalling lymphocyte activation molecule (SLAM). The MV-H glycoprotein from an Edmonston MV variant and the MV-binding fragment of the CD46 receptor were overproduced in mammalian cells and used to crystallize an MV-H-CD46 complex. Well diffracting crystals containing two complexes in the asymmetric unit were obtained and the structure of the complex was solved by the molecular-replacement method.
Santiago
César
C
Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma, 28049 Madrid, Spain.
Gutiérrez-Rodríguez
Angel
A
Tucker
Paul A
PA
Stehle
Thilo
T
Casasnovas
José M
JM
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006389:Hemagglutinins, Viral / Q000737:chemistry*; D006801:Humans; D008459:Measles virus; D051916:Membrane Cofactor Protein / Q000737:chemistry*; D011991:Receptors, Virus / Q000737:chemistry*
D006389:Hemagglutinins, Viral; D051916:Membrane Cofactor Protein; D011991:Receptors, Virus