A two-photon fluorescent probe for thiols in live cells and tissues.
We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90-180 microm depth without interference from other biologically relevant species by two-photon microscopy.
Lee
Jun Han
JH
Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
Lim
Chang Su
CS
Tian
Yu Shun
YS
Han
Ji Hee
JH
Cho
Bong Rae
BR
D000818:Animals; D001923:Brain Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008744:Methylamines / Q000737:chemistry; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry; D017785:Photons; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis*
C548238:2-methylamino-6-acetylnaphthalene; D005456:Fluorescent Dyes; D008744:Methylamines; D009281:Naphthalenes; D013438:Sulfhydryl Compounds