Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry.
Flow cytometry is an ever-advancing high-throughput multivariate analysis tool that natively provides size and morphological information. To obtain molecular information, however, typically requires the addition of fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. A complementary or alternative, label-free approach to molecular information is through multiplex coherent anti-Stokes Raman scattering (MCARS), which is a coherent, nonlinear optical method that provides a wealth of molecular information by probing the Raman energies within a molecule. In this work, we demonstrate the unique capability of our MCARS flow cytometer to distinguish flowing particles and discuss system performance capabilities and possibilities.
Camp
Charles H
CH
School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Dr., Atlanta, GA, 30332, USA. ccampjr@ece.gatech.edu
Yegnanarayanan
Siva
S
Eftekhar
Ali A
AA
Sridhar
Hamsa
H
Adibi
Ali
A
D001704:Biopolymers / Q000032:analysis*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013194:Staining and Labeling
D001704:Biopolymers