title: Raman spectroscopy of Xenopus laevis oocytes.
abstract: This work reports on the application of Raman spectroscopy for the analysis of Xenopus laevis oocytes (stage-I). A two-color home-made microscope has been used for this investigation. In particular, a 785nm Raman probe has been used to acquire the spontaneous Raman scattering from the oocyte cytoplasm, while a 532nm probe has been employed to detect carotenoids through Resonant Raman Scattering. Finally, the distribution of beta-carotene along a diameter of a single oocyte has been investigated.
authors.affiliation: University of Naples Federico II, Department of Physics, via Cinthia, Napoli, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Rusciano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pesce
authors.forename: Marinella
authors.initials: M
authors.lastname: Salemme
authors.forename: Lara
authors.initials: L
authors.lastname: Selvaggi
authors.forename: Carmen
authors.initials: C
authors.lastname: Vaccaro
authors.forename: Antonio
authors.initials: A
authors.lastname: Sasso
authors.forename: Rosa
authors.initials: R
authors.lastname: Carotenuto
mesh_terms: D000465:Algorithms; D000818:Animals; D002338:Carotenoids / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D004867:Equipment Design; D005260:Female; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods; D009865:Oocytes / Q000378:metabolism*; D010777:Photochemistry / Q000379:methods; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D002338:Carotenoids