title: Effect of carbon dioxide on broth microdilution susceptibility testing of Brucella spp.
abstract: Since some strains of Brucella species may require carbon dioxide for growth, a multilaboratory study was conducted to compare broth microdilution susceptibility results using ambient air (AA) and 5% CO2 incubation conditions. Six antimicrobial agents were tested against 39 Brucella isolates. Aminoglycoside MICs tended to be 1 log2 dilution higher in CO2 than in AA; tetracycline-class MICs to be 1 log2 dilution lower in CO2.
authors.affiliation: Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. dul7@cdc.gov
authors.forename: David R
authors.initials: DR
authors.lastname: Lonsway
authors.forename: Laura A
authors.initials: LA
authors.lastname: Jevitt
authors.forename: James R
authors.initials: JR
authors.lastname: Uhl
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Cockerill
authors.forename: Mary E
authors.initials: ME
authors.lastname: Anderson
authors.forename: Maureen M
authors.initials: MM
authors.lastname: Sullivan
authors.forename: Barun K
authors.initials: BK
authors.lastname: De
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Edwards
authors.forename: Jean B
authors.initials: JB
authors.lastname: Patel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002002:Brucella / Q000187:drug effects* / Q000254:growth & development; D002245:Carbon Dioxide / Q000378:metabolism*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D002245:Carbon Dioxide