title: Inulin-coated butyrate increases ileal MCT1 expression and affects mucosal morphology in the porcine ileum by reduced apoptosis.
abstract: Carbohydrates, which were not digested in the jejunum, will be fermented by micro-organisms to short chain fatty acids. These are transported by the monocarboxylate transporter 1 (MCT1) through the gut wall and serve as fuels for colonic cells. To deliver butyrate to the distal part of the intestine, inulin with a low precaecal digestibility was chosen as a coating material. Approximately 150 g of inulin-coated butyrate (containing 81 g butyrate) per day was fed to pigs (mean weight: 97 kg) over a period of 6 days after an adaptation period of 6 days with linear increasing amounts of butyrate. The following observations compared to controls were observed: (1) coating was digested microbially in the ileum; (2) MCT1-mRNA showed a higher expression in the ileum; (3) apoptosis was reduced in the ileum but mitosis was not changed; and (4) length of villi increased by approximately 25% in the ileum. Feeding inulin-coated butyrate resulted in an increased ileal surface. Delivery of butyrate to the colon requires a more resistant inulin-coating.
authors.affiliation: R-Biopharm, Darmstadt, Germany. m.lacorn@r-biopharm.de
authors.forename: M
authors.initials: M
authors.lastname: Lacorn
authors.forename: M
authors.initials: M
authors.lastname: Goerke
authors.forename: R
authors.initials: R
authors.lastname: Claus
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D020148:Butyric Acid / Q000737:chemistry / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D005786:Gene Expression Regulation / Q000187:drug effects*; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects; D007444:Inulin / Q000737:chemistry* / Q000378:metabolism*; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D020148:Butyric Acid; D007444:Inulin