title: Isolation of amniotic epithelial stem cells.
abstract: Many of the cell types that can be isolated from placental tissues retain phenotypic plasticity that makes them an interesting source of cells for regenerative medicine. Several procedures for the isolation of stem cells from different parts of the placenta have been reported. This unit describes a detailed and simple protocol for the selective isolation of amniotic epithelial cells from human term placenta without disturbing the mesenchymal layer. We also introduce a simple density separation technique for the enrichment of the population for SSEA-4 positive cells.
authors.affiliation: University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Toshio
authors.initials: T
authors.lastname: Miki
authors.forename: Fabio
authors.initials: F
authors.lastname: Marongiu
authors.forename: Kenneth
authors.initials: K
authors.lastname: Dorko
authors.forename: Ewa C S
authors.initials: EC
authors.lastname: Ellis
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Strom
mesh_terms: D000650:Amnion / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers