title: Identification of binding proteins of fusarielin A as actin and tubulin.
abstract: Fusarielin A (FSA), isolated from culture of Fusarium sp. K432, has anti-fungal/anti-angiogenic activity. Based on our previous structure-activity relationship studies, we prepared FSA-immobilized affinity gel. The results of affinity chromatography with this gel indicated that tubulin and actin are FSA-binding proteins. The binding affinities of tubulin and actin for FSA were estimated by the use of surface plasmon resonance spectroscopy.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Noguchi-Yachide
authors.forename: Kosuke
authors.initials: K
authors.lastname: Dodo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Aoyama
authors.forename: Haruka
authors.initials: H
authors.lastname: Fujimoto
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hori
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000595:Amino Acid Sequence; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004852:Epoxy Compounds / Q000302:isolation & purification / Q000494:pharmacology; D005670:Fusarium / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000302:isolation & purification / Q000494:pharmacology; D011485:Protein Binding; D014404:Tubulin / Q000032:analysis / Q000378:metabolism*
chemical_list: D000199:Actins; D020533:Angiogenesis Inhibitors; D000935:Antifungal Agents; D004852:Epoxy Compounds; D009281:Naphthalenes; D014404:Tubulin; C092174:fusarielin A