Increased expression of cellular retinol-binding protein 1 in laryngeal squamous cell carcinoma.
OBJECTIVE
To investigate the genomic alterations in larynx carcinomas (LaCa) tissues and its prognostics values in predicting survival.


METHODS
To analyse the aberrations in the genome of LaCa patients, we used array comparative genomic hybridization in 19 human laryngeal tumour samples. DNA samples were also subjected to detect human papillomavirus (HPV) sequences by polymerase chain reaction (PCR). Copy number gain was confirmed by real-time PCR. The cellular retinol-binding protein 1 (CRBP-1) gene expression was also confirmed by immunohistochemistry assay on LaCa tissues. To identify prognostic feature, CRBP-1 gene gain was correlated to patient survival.


RESULTS
The most common gains were detected for CRBP-1 and EGFR genes, while DNA lost in RAF-1 gene. Immunohistochemistry assay was revealed strong expression of CRBP1 protein in those cases with CRBP-1 gene gain. The CRBP-1 gene gain and its expression correlated significantly with survival (P = 0.003). Cox regression analysis indicated that CRBP-1 expression level was a factor of survival (P = 0.008). HPV sequences were detected in 42% of the samples, and did not show any relationship with specific gene alterations.


CONCLUSIONS
Our data shows that CRBP-1 gene gain can be determined by immunohistochemistry on routinely processed tissue specimens, and could support as a potential novel marker for long-term survival in laryngeal squamous cell carcinoma.
Peralta
Raúl
R
Oncology Research Unit, Hospital Oncologia, Health Research Coordination, IMSS, Mexico City, Mexico.
Baudis
Michael
M
Vazquez
Guelaguetza
G
Juárez
Sergio
S
Ortiz
Rocío
R
Decanini
Horacio
H
Hernandez
Dulce
D
Gallegos
Francisco
F
Valdivia
Alejandra
A
Piña
Patricia
P
Salcedo
Mauricio
M
D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality; D055028:Comparative Genomic Hybridization; D066246:ErbB Receptors / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000401:mortality; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D016019:Survival Analysis; D015854:Up-Regulation
D014408:Biomarkers, Tumor; C448939:CRB1 protein, human; D005136:Eye Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D066246:ErbB Receptors