title: Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1.
abstract: The Human Proteome Organisation Human Disease Glycomics/Proteome Initiative recently coordinated a multi-institutional study that evaluated methodologies that are widely used for defining the N-glycan content in glycoproteins. The study convincingly endorsed mass spectrometry as the technique of choice for glycomic profiling in the discovery phase of diagnostic research. The present study reports the extension of the Human Disease Glycomics/Proteome Initiative's activities to an assessment of the methodologies currently used for O-glycan analysis. Three samples of IgA1 isolated from the serum of patients with multiple myeloma were distributed to 15 laboratories worldwide for O-glycomics analysis. A variety of mass spectrometric and chromatographic procedures representative of current methodologies were used. Similar to the previous N-glycan study, the results convincingly confirmed the pre-eminent performance of MS for O-glycan profiling. Two general strategies were found to give the most reliable data, namely direct MS analysis of mixtures of permethylated reduced glycans in the positive ion mode and analysis of native reduced glycans in the negative ion mode using LC-MS approaches. In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful.
authors.affiliation: Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan. waday@mch.pref.osaka.jp
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Wada
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
authors.forename: Bérangère
authors.initials: B
authors.lastname: Tissot
authors.forename: Kévin
authors.initials: K
authors.lastname: Canis
authors.forename: Parastoo
authors.initials: P
authors.lastname: Azadi
authors.forename: Malin
authors.initials: M
authors.lastname: Bäckström
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
authors.forename: Gunnar C
authors.initials: GC
authors.lastname: Hansson
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hiki
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishihara
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ito
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
authors.forename: Niclas
authors.initials: N
authors.lastname: Karlsson
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Hayes
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Nana
authors.initials: N
authors.lastname: Kawasaki
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Daniel
authors.initials: D
authors.lastname: Kolarich
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kondo
authors.forename: Carlito
authors.initials: C
authors.lastname: Lebrilla
authors.forename: Miyako
authors.initials: M
authors.lastname: Nakano
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Jan
authors.initials: J
authors.lastname: Novak
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: Erina
authors.initials: E
authors.lastname: Ohno
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Palaima
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Renfrow
authors.forename: Michiko
authors.initials: M
authors.lastname: Tajiri
authors.forename: Kristina A
authors.initials: KA
authors.lastname: Thomsson
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yagi
authors.forename: Shin-Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000465:Algorithms; D002240:Carbohydrate Sequence; D004194:Disease / Q000209:etiology; D054794:Glycomics / Q000379:methods* / Q000458:organization & administration / Q000592:standards; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D055442:Metabolome; D008954:Models, Biological; D011134:Polysaccharides / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods* / Q000458:organization & administration* / Q000592:standards; D012958:Societies, Scientific / Q000458:organization & administration
chemical_list: D006023:Glycoproteins; D007070:Immunoglobulin A; D011134:Polysaccharides; D020543:Proteome