[Apoptosis of multiple myeloma cells induced by gossypol acetic acid in vitro and its mechanism].
This study was aimed to investigate the apoptosis effect of gossypol acetic acid on classic human multiple myeloma RPMI8226 cell line in vitro and its mechanism. The inhibitory effect on proliferation of RPMI8226 cells was evaluated by means of MTT assay. Cytotoxic effect and apoptosis was identified and analyzed with the aid of transmission electron microscopy, mitochondria membrane potential (MMP) and DNA gel electrophoresis. Meanwhile, Western-blot assay was used to detect the changes of several key cell apoptosis regulatory proteins such as BAX, caspase-3 and caspase-8 in these cells before and after treatment. The results showed that low concentrations of gossypol acetic acid (> 16 micromol/L) could suppress the proliferation and induce the apoptosis in RPMI8226 cells effectively. At the same time, gossypol acetic acid could also down-regulate the mitochondrial membrane potential, up-regulate the expression of the apoptosis-related protein such as BAX and caspase-3. It is concluded that the gossypol acetic acid can selectively induce proliferation inhibition and apoptosis of multiple myeloma RPMI8226 cells with a smaller dose.
Wang
Wen-Qing
WQ
Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710033, Shaanxi Province, China.
Li
Rong
R
Bai
Qing-Xian
QX
Zhang
Wei-Ping
WP
Wang
Juan-Hong
JH
Wang
Zhe
Z
Chen
Xie-Qun
XQ
Huang
Gao-Sheng
GS
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D009101:Multiple Myeloma / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494826:BAX protein, human; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D006072:Gossypol; C028178:gossypol acetic acid