Natural killer cell conjugate assay using two-color flow cytometry.
This flow cytometry-based method is a quick way to detect adhesion of NK cells to target cells. The two cell types are labeled with distinct fluorescent dyes and following co-incubation, the number of NK cells firmly adhered to target cells is quantified using two-color flow cytometry.
Burshtyn
Deborah N
DN
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada.
Davidson
Chelsea
C
D002448:Cell Adhesion; D002460:Cell Line; D003116:Color; D005434:Flow Cytometry / Q000379:methods*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling