title: Highly sequence-dependent formation of fluorescent silver nanoclusters in hybridized DNA duplexes for single nucleotide mutation identification.
abstract: Fluorescent silver nanoclusters were successfully synthesized using hybridized DNA duplexes as capping scaffolds. The formation of these emitters was highly sequence-dependent and could specifically identify a single nucleotide mutation, the sickle cell anemia gene mutation. Furthermore, the identification of single-nucleotide differences using this strategy was extended to more general types of single-nucleotide mismatches.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, and Graduate School of the Chinese Academy of Sciences, Changchun 130022, Jilin, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.forename: Jipei
authors.initials: J
authors.lastname: Yuan
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Dong
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D000755:Anemia, Sickle Cell / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005453:Fluorescence; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D017354:Point Mutation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D004247:DNA