title: Flavonoids from the buds of Rosa damascena inhibit the activity of 3-hydroxy-3-methylglutaryl-coenzyme a reductase and angiotensin I-converting enzyme.
abstract: Rosa damascena has been manufactured as various food products, including tea, in Korea. A new flavonoid glycoside, kaempferol-3-O-beta-D-glucopyranosyl(1-->4)-beta-D-xylopyranoside, named roxyloside A was isolated from the buds of this plant, along with four known compounds, isoquercitrin, afzelin, cyanidin-3-O-beta-glucoside, and quercetin gentiobioside. The chemical structures of these compounds were determined by spectroscopic analyses, including FAB-MS, UV, IR, (1)H and (13)C NMR, DEPT, and 2D NMR (COSY, HSQC, and HMBC). All the isolated compounds except cyanidin-3-O-beta-glucoside exhibited high levels of inhibitory activity against 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase with IC(50) values ranging from 47.1 to 80.6 microM. Cyanidin-3-O-beta-glucoside significantly suppressed angiotensin I-converting enzyme (ACE) activity, with an IC(50) value of 138.8 microM, while the other four compounds were ineffective. These results indicate that R. damascena and its flavonoids may be effective to improve the cardiovascular system.
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, South Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kwon
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Hyungjae
authors.initials: H
authors.lastname: Lee
authors.forename: Dae-Ok
authors.initials: DO
authors.lastname: Kim
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Young-Eon
authors.initials: YE
authors.lastname: Kim
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000214:Acyl Coenzyme A / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D007700:Kinetics; D008168:Lung / Q000737:chemistry / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*
chemical_list: D000214:Acyl Coenzyme A; D000806:Angiotensin-Converting Enzyme Inhibitors; D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D007703:Peptidyl-Dipeptidase A