Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography.
Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.
Ju
Xiuyun
X
Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China.
Sun
Yong
Y
Cao
Xiaoying
X
Jiang
Jihong
J
Zhang
Tianyou
T
Ito
Yoichiro
Y