Comparison of the enteric mucosal immunomodulatory activity of combinations of Coptis chinensis Franch. Rhizomes and Evodia rutaecarpa (Juss.) Benth. Fruits in mice with dextran sulphate sodium-induced ulcerative colitis.
Combinations of crude rhizomes of Coptis chinensis Franch., Ranunculaceae, and fruits of Evodia rutaecarpa (Juss.) Benth., Rutaceae, at a ratio of 6 : 1 (formula A) and 1 : 6 (formula B) were extracted with boiling water, and their modulatory activity on enteric mucosal immune responses in mice with dextran sulphate sodium-induced ulcerative colitis was investigated. The results showed that both formulas could reduce the severity of inflammation in the colon. Formula A at a low dose can decrease myeloperoxidase (MPO) activity, and formula B was inactive. Both formulas did not affect the percentages of CD4 (+) and CD8 (+) T cells in the periphery, but they evoked an increase of CD8 (+) T cells among the enteric intraepithelial lymphocytes. Formula B at a low dose could increase both CD4 (+) and CD8 (+) cells, and formula A at a high dose could only increase CD8 (+) T cells among the Peyer's patch lymphocytes (p < 0.05). Both formulas did not affect the percentages of CD4 (+) and CD8 (+) T cells among the lamina propria lymphocytes, but decreased the serum concentration of IL-1 beta (p < 0.05 at a low dose of formula A) and enhanced the level of IL-10 in serum (p < 0.05 at a low dose formula B). We conclude that both formulas have a similar modulating effect on enteric mucosal immune responses, the major difference being that formula A could decrease the level of IL-1 beta, while formula B could increase the IL-10 dose in serum.
Luo
Yingying
Y
Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, PR China.
Zhao
Hongyan
H
Liu
Zhenli
Z
Ju
Dahong
D
He
Xiaojuan
X
Xiao
Cheng
C
Zhong
Guoyue
G
Chen
Shilin
S
Yang
Dajian
D
Chan
Albert Sun-Chi
AS
Lu
Aiping
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000276:immunology; D016264:Dextran Sulfate / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D018928:Immunity, Mucosal / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D029626:Ranunculaceae / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027821:Rutaceae / Q000737:chemistry*
D010936:Plant Extracts; D016264:Dextran Sulfate