Investigation of multiple forms of Tri a Bd 27K, a major wheat allergen, by immunoblotting analysis.
Tri a Bd 27K, a major wheat allergen, is a glycoprotein. Tri a Bd 27K was found to occur in multiple forms by two-dimensional polyacrylamide gel electrophoresis and immunoblotting with a monoclonal antibody against the allergen. Furthermore, it was found that only Tri a Bd 27K components, which have N-linked glycan moieties with fucose residues, bound to IgE antibodies in the sera of wheat-sensitive patients.
Hiemori
Miki
M
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Japan. hiemori@fhw.oka-pu.ac.jp
Yosida
Yukari
Y
Kimoto
Masumi
M
Yamashita
Hiromi
H
Takahashi
Kyoko
K
Takahashi
Kiyoshi
K
Komiyama
Nobuko
N
Tsuji
Hideaki
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008925:Mites / Q000276:immunology; D011134:Polysaccharides / Q000378:metabolism; D014908:Triticum / Q000276:immunology*; D021182:Wheat Hypersensitivity / Q000097:blood / Q000276:immunology
D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D011134:Polysaccharides; C539494:Tri a Bd 27K allergen, Triticum aestivum