title: Growth in Acanthamoeba sp. and antibiotic susceptibility of Legionella micdadei isolated from hot spring water samples.
abstract: As part of an epidemiological study on legionellosis, we attempted to isolate Legionella spp. from hot spring water samples, and were able to isolate Legionella micdadei from 3 (5.5%) of 55 samples. All of these isolates were able to grow within Acanthamoeba sp., suggesting that the isolates will be pathogens. We also confirmed that the K-2 strain from hot spring water grew in guinea pig monocytes. Sensitivity tests using 10 drugs showed that the isolates were most sensitive to imipenem, with the MIC90 of 0.032 microg/ml, were least sensitive to minocycline, with the MIC90 of 4 microg/ml, and were not sensitive to low amounts of other drugs.
authors.affiliation: School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa 229-8501, Japan. furuhata@azabu-u.ac.jp
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furuhata
authors.forename: Kikumi
authors.initials: K
authors.lastname: Ogihara
authors.forename: Rumi
authors.initials: R
authors.lastname: Okuno
authors.forename: Kenji
authors.initials: K
authors.lastname: Oonaka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuyama
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects / Q000382:microbiology*; D000818:Animals; D006168:Guinea Pigs; D045482:Hot Springs / Q000382:microbiology*; D007875:Legionella / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D007876:Legionellosis / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D014871:Water Microbiology