[Peculiarities of growth and development of cultured mucosal cells from the upper respiratory tract stimulated by growth factors].
Specific features of growth and development of cultured mucosal cells from the upper respiratory tract were studied during their in vitro stimulation by keratinocyte growth factor (KGF) and epidermal growth factor (EGF). Phenotypic composition and quantitative characteristics of cultured epithelial cells was investigated with the use of monoclonal CD49F antibodies and flow cytofluorometry. The culture technique makes it possible to obtain a large amount of cells for the evaluation of their pathological changes. Moreover, cell cultures can be used to restore lesioned mucosa of the upper respiratory tract both in experiment and under clinical conditions.
Chekan
V L
VL
Kvacheva
Z B
ZB
Petrova
L G
LG
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008856:Microscopy, Fluorescence; D010641:Phenotype; D011817:Rabbits; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
D051523:Fibroblast Growth Factor 7; D004815:Epidermal Growth Factor