title: [Primary study on mechanism of baicalin on the Th1/Th2 response in murine model of asthma].
abstract: OBJECTIVE
  To study the mechanism of baicalin on the cytokines of Th1/Th2 in murine model of asthma.
  
  
  METHODS
  The murine model of asthma was induced by OVA. Different doses of baicalin were orally administered to the mice respectively. The spleen cells were cultured 3 days for the measurement of IFN-gamma, IL-4, IL-5 and IL-10 by ELISA. After 2 days of culture, the spleen cells were treated with Trizol for extraction of total RNA. The gene expressions of T-bet, GATA-3 and STAT-6 were analyzed by RT-PCR.
  
  
  RESULTS
  The treatment with baicalin obviously decreased the production of IL-4 and IL-5 and the gene expression of GATA-3, STAT-6, but increased the production of IL-10.
  
  
  CONCLUSIONS
  Baicalin may modulate the Th1/Th2 balance mainly by altering the gene expressions of GATA-3 and STAT-6 in vivo and increasing the production of IL-10.
authors.affiliation: College of Herbal Pharmacy, Jinan University, Guangzhou 510632, China. hftxyy@yahoo.com.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Tong
authors.forename: Hua-ming
authors.initials: HM
authors.lastname: Deng
authors.forename: Hong
authors.initials: H
authors.lastname: Nie
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050800:STAT6 Transcription Factor / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D005419:Flavonoids; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; C038044:baicalin; D010047:Ovalbumin