Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane.
In order to prevent biofilm formation by Candida albicans, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of C. albicans biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against C. albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent.
De Prijck
Kristof
K
Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
De Smet
Nele
N
Rymarczyk-Machal
Monika
M
Van Driessche
Gonzalez
G
Devreese
Bart
B
Coenye
Tom
T
Schacht
Etienne
E
Nelis
Hans J
HJ
D000935:Antifungal Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D003432:Cross-Linking Reagents; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D054808:Histatins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods; D010455:Peptides / Q000737:chemistry*
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D003432:Cross-Linking Reagents; C504850:Dhvar-5; D004129:Dimethylpolysiloxanes; D054808:Histatins; D010455:Peptides; C013830:baysilon