Physiological fluorescence lifetime imaging microscopy improves Förster resonance energy transfer detection in living cells.
Accurate, unambiguous detection of molecular interactions in living cells via measurements of Forster (or fluorescence) resonance energy transfer (FRET) events is experimentally challenging. We develop and apply a physiological fluorescence lifetime imaging microscopy (physiological FLIM) system to significantly improve FRET detection in living cells. Multiple positive and negative cellular controls are implemented to validate the experimental method developed. FLIM measurement techniques were found to remove fluorescence intensity-based artifacts, resulting in a seven-fold improvement in fluorescence measurement precision. The addition of cellular environmental controls, including both temperature and CO(2) stabilization, for physiological FLIM eliminates nonspecific FRET in the live-cell system studied. Overall, only physiological FLIM results in statistically significant results that clearly indicated the presence of specific molecular interactions in the live-cell system. This approach can be applied generally to improve the accuracy and precision of FRET measurements in living cells.
Chang
Ching-Wei
CW
Wu
Mei
M
Merajver
Sofia D
SD
Mycek
Mary-Ann
MA
D000818:Animals; D002468:Cell Physiological Phenomena; D002478:Cells, Cultured; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature; D020741:rho GTP-Binding Proteins / Q000502:physiology
D020741:rho GTP-Binding Proteins