title: Increased serum concentration of immune cell derived microparticles in polymyositis/dermatomyositis.
abstract: Microparticles are recently recognized players of intercellular communication. They are involved in signal transduction, cell activation and apoptosis. Their importance is also suggested in autoimmune diseases such as rheumatoid arthritis and systemic sclerosis. We investigated the role of microparticles in polymyositis/dermatomyositis, a group of rare autoimmune diseases, characterized by specific skin lesions and muscle weakness. The plasma concentration of monocyte and lymphocyte derived microparticles of 20 patients with polymyositis/dermatomyositis and 20 healthy controls were determined by flow cytometry. The structure of microparticles was visualized by electron microscopy. Significantly elevated numbers of monocyte (CD14 positive), T-lymphocyte (CD3 positive) and B-lymphocyte (CD19 positive) derived microparticles were found in the plasma samples of polymyositis/dermatomyositis patients, compared to healthy controls (p=0.001, 0.01 and 0.006, respectively). Furthermore, the plasma levels of monocyte and B-lymphocyte derived microparticles correlated with the manual muscle strength test (r=0.497, p=0.027; r=0.508, p=0.023; respectively). Patients with anti-Jo-1 antibody and lung involvement had significantly higher numbers of T- and B-lymphocyte and monocyte derived MPs (p=0.006, 0.012 and 0.007, respectively, for anti-Jo-1; p=0.013, 0.016 and 0.025, respectively, for lung involvement). After ultracentrifugation, CK activity could be detected only in traces in the resuspended pellet containing microparticles of healthy and diseased individuals. The electron microscopy revealed slightly different microparticles in the samples of patients with polymyositis/dermatomyositis. These results suggest that immune cell derived microparticles may contribute to the inflammatory process in polymyositis/dermatomyositis, however, CK-positive, possibly muscle derived microparticles do not seem to be present in the blood of patients with polymyositis/dermatomyositis.
authors.affiliation: Department of Genetics, Semmelweis University, Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Baka
authors.forename: Ladislav
authors.initials: L
authors.lastname: Senolt
authors.forename: Jiri
authors.initials: J
authors.lastname: Vencovsky
authors.forename: Herman
authors.initials: H
authors.lastname: Mann
authors.forename: Piroska Sebestyén
authors.initials: PS
authors.lastname: Simon
authors.forename: Agnes
authors.initials: A
authors.lastname: Kittel
authors.forename: Edit
authors.initials: E
authors.lastname: Buzás
authors.forename: György
authors.initials: G
authors.lastname: Nagy
mesh_terms: D000974:Antibodies, Antinuclear / Q000097:blood; D001402:B-Lymphocytes / Q000378:metabolism*; D055252:Cell-Derived Microparticles / Q000378:metabolism* / Q000648:ultrastructure; D003402:Creatine Kinase / Q000097:blood; D003882:Dermatomyositis / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D017285:Polymyositis / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000974:Antibodies, Antinuclear; C046750:Jo-1 antibody; D003402:Creatine Kinase