Characterization and expression of cry4Cb1 and cry30Ga1 from Bacillus thuringiensis strain HS18-1.
We characterized a novel Bacillus thuringiensis isolate native to China (HS18-1) that shows a spherical crystal harboring two major proteins of about 70 and 130kDa, and contains three novel cry genes (cry4Cb1, cry30Ga1, cry54-type). Furthermore, the cry4Cb1 and cry30Ga1 genes were expressed in Escherichia coli BL21 (DE3): pLysS. Insecticidal activity tests showed that the cry4Cb1 protein exhibited larvicidal activity against Aedes aegypti (Diptera) and the cry30Ga1 protein was toxic to both A. aegypti and P. xylostella (Lepidoptera).
Zhu
Jun
J
Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China.
Zheng
Aiping
A
Wang
Shiquang
S
Liu
Huainian
H
Li
Ping
P
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008855:Microscopy, Electron, Scanning; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins