The effect of phytohormones on safflower regeneration plant.
OBJECTIVE
To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation.


METHODS
The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones.


RESULTS
1. Different hormones by single using induced calli, but didn't directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium: MS + 3% Sucrose + 0.7% Agar + 1.0 mg/L NAA; differentiation medium: MS + 3% Sucrose + 0.7% Agar + 0.2 mg/L NAA + 1.0 mg/L 6-BA; rooting medium: 1/4 MS + 2.0 mg/L NAA + 0.5 mg/L IAA.


CONCLUSIONS
We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.
Yang
Jing
J
Ministry of Education Bioreactor and Drug Development Research Center, Jilin Agricultural University, Changchun 130118, China. xiziyang512@163.com
Xiong
Li-dong
LD
Li
Tian-hang
TH
Li
Hai-yan
HY
Qu
Qing
Q
Fu
Hong-qi
HQ
Liu
Xiu-ming
XM
Li
Xiao-kun
XK
D031187:Carthamus tinctorius / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators