c-myb activates CXCL12 transcription in T47D and MCF7 breast cancer cells.
Chemokine C-X-C motif ligand 12 (CXCL12) is a potent chemotactic and angiogenic factor that has been proposed to play a role in organ-specific metastasis and angiogenic activity in several malignancies. In this study, we found that the overexpression of c-myb could elevate CXCL12 mRNA level and CXCL12 promoter activity in human T47D and MCF-7 breast cancer cells. Chromatin immunoprecipitation assay demonstrated that c-myb could bind to the CXCL12 promoter in the cells transfected with cmyb expression vector. c-myb siRNA attenuated CXCL12 promoter activity and the binding of c-myb to the CXCL12 promoter in T47D and MCF-7 cells. These results indicated that c-myb could activate CXCL12 promoter transcription.
Chen
Li
L
Department of Breast Surgery, Breast Cancer Institute, Cancer Hospital/Cancer Institute, Fudan University, Shanghai, China.
Xu
Siguang
S
Zeng
Xiaohua
X
Li
Junjie
J
Yin
Wenjin
W
Chen
Ying
Y
Shao
Zhimin
Z
Jin
Wei
W
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011401:Promoter Regions, Genetic / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb / Q000494:pharmacology* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects*
D054377:Chemokine CXCL12; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger