Effective siRNAs inhibit the replication of novel influenza A (H1N1) virus.
In March and April 2009, an entirely novel influenza A (H1N1) virus (NIAV) emerged in Mexico and the USA. During the subsequent months, the virus rapidly spread all over the world by person-to-person transmission. In this report, RNA interference (RNAi) was used as an antiviral agent to inhibit NIAV replication in A549 cells. Ten small interfering RNAs (siRNAs) targeting extremely conserved regions among multiple NIAV genomes could effectively block the replication of NIAV strain A/Beijing/01/2009 (H1N1) in A549 cells. This study may be useful to confront the sudden emergence of NIAV infection.
Zhiqiang
Wu
W
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing 100176, PR China.
Yaowu
Yang
Y
Fan
Yang
Y
Jian
Yang
Y
Yongfeng
Hu
H
Lina
Zhao
Z
Jianwei
Wang
W
Qi
Jin
J
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D034741:RNA, Small Interfering