title: Dietary supplementation with arachidonic acid but not eicosapentaenoic or docosahexaenoic acids alter lipids metabolism in C57BL/6J mice.
abstract: In order to investigate the effects of dietary supplementation rich in omega 3 and omega 6 fatty acids, we set up an experiment of twenty four C57BL/6J male mice segregated into 3 groups: normal diet (ND), omega 3 polyunsaturated fatty acid (n-3 PUFA,) and omega 6 (n-6 PUFA). At the end of the experiment that lasted for 1 month, food consumption of ND and n-3 PUFA were similar while it decreased in n-6 PUFA group. Total cholesterol, triglycerides, free fatty acids, and phospholipids profiles were increased in n-6 PUFA. LDL decreased in n-3 PUFA while increased in n-6 PUFA fed mice comparing to control group. On the other hand, there was no difference between treatments in HDL and glucose levels. Expression of leptin (ob) gene transcripts in epididymal fat were significantly elevated in n-6 PUFA mice compared to ND and n-3 PUFA groups while hypothalamic ob receptor A (obRa) mRNA did not changed in response to diet regimes. Transmission and scanning electron microscopy showed different degrees in fatty changes in the liver of both PUFA groups including lipid droplet infiltration and Ito cells with over accumulated lipids. In conclusion, under PUFA dietary supplementation, the hyperlipidemic status and elevated ob expression of n-6 PUFA but not n-3 PUFA fed mice suggests altered lipid metabolism between PUFA groups and/or different endocrine involvement. Moreover, the coincidently structural changes observed in liver of this group direct us to call for further studies to investigate the anti-obesity effect and safety of these PUFA under high supplementation condition.
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Egypt. samehmagd@med.niigata-u.ac.jp
authors.forename: Sameh
authors.initials: S
authors.lastname: Magdeldin
authors.forename: Yaser
authors.initials: Y
authors.lastname: Elewa
authors.forename: Takako
authors.initials: T
authors.lastname: Ikeda
authors.forename: Junko
authors.initials: J
authors.lastname: Ikei
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nameta
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujinaka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Eishin
authors.initials: E
authors.lastname: Yaoita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage* / Q000378:metabolism; D001769:Blood / Q000378:metabolism; D001835:Body Weight; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000378:metabolism; D004435:Eating; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage* / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D054411:Receptors, Leptin / Q000378:metabolism
chemical_list: D004042:Dietary Fats, Unsaturated; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D020738:Leptin; D054411:Receptors, Leptin; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases