Studying NK cell/dendritic cell interactions.
Although NK cells were originally identified as "naturally" active cells believed to follow a cell-autonomous activation program, it is now widely accepted that NK cells need to interact with dendritic cells for their full functional activation and for their homeostasis. In this chapter, we will provide an experimental guide to the analysis of NK cell/DC interactions in vitro and in vivo. We have put special emphasis on the recently developed mouse models allowing the inducible and specific ablation of various subsets of DCs and other myeloid cells.
Lucas
Mathias
M
Institute of Medical Microbiology & Hygiene, University of Freiburg Medical Center, Freiburg, Germany.
Vonarbourg
Cedric
C
Aichele
Peter
P
Diefenbach
Andreas
A
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002450:Cell Communication; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005818:Genetic Engineering; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008954:Models, Biological; D022423:Myeloid Cells / Q000378:metabolism; D013194:Staining and Labeling; D051193:Toll-Like Receptors / Q000378:metabolism
D015415:Biomarkers; D016207:Cytokines; C587883:HBEGF protein, human; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D051193:Toll-Like Receptors