title: [Synthesis and identification of artificial antigen to the common moiety of some organophosphorus pesticides].
abstract: OBJECTIVE
  To develop an immunoassay for multiple residues of organophosphorus pesticides.
  
  
  METHODS
  The generic hapten O, O-dimethyl-O-(4-carboxyphenyl)ester (HP) was synthesized by the reaction between O, O-Dimethyl phosphorochloridothionate and 4-hydroxybenzoic acid methyl ester, and then conjugated to bovine serum albumin (BSA) by active ester method for immunogens and ovalbumin (OVA) by mixed anhydride reaction for coating antigens.
  
  
  RESULTS
  The hapten was synthesized successfully, which was characterized by MS. The coupling ratios were 15.6:1 (HP-BSA) and 16.2:1(HP-OVA), respectively, calculated by UV spectrophotometry.
  
  
  CONCLUSIONS
  suggested that the artificial antigens could be used for production of broad specificity anti-organophosphorous pesticides antibody.
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. jiangpan_19841204@163.com
authors.forename: Panpan
authors.initials: P
authors.lastname: Jiang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.forename: Jiguo
authors.initials: J
authors.lastname: He
mesh_terms: D000941:Antigens / Q000737:chemistry*; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000276:immunology; D010047:Ovalbumin / Q000737:chemistry; D010575:Pesticides / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000941:Antigens; D006241:Haptens; D009943:Organophosphorus Compounds; D010575:Pesticides; D012710:Serum Albumin, Bovine; D010047:Ovalbumin