title: 1H NMR-based metabolic profiling of urine from children with nephrouropathies.
abstract: Pediatric nephrourological diseases are associated with functional alterations frequently related to inflammatory states. A feedback loop adjusts urinary system function while forcing adaptation to internal and external influences during disease development and as a result of treatment. We hypothesized that nephrourological dysfunction would alter the urine metabolite pattern in children in a defined manner. To characterize the metabolite patterns associated with nephrouropathies, a proton nuclear magnetic resonance (1H NMR)-based metabonomic analysis was performed on urine samples obtained from twenty-one children affected by nephrouropathies and 19 healthy controls. Urine samples were analyzed with a 400 MHz Varian spectrometer and multivariate statistical techniques were applied for data interpretation. Linear discriminant analysis-based classification of the spectral data demonstrated high accuracy (95 per cent) in the separation of the two groups of samples. The urine metabolite profiles were shown to correlate with nephrourological disorders in our model. In conclusion, 1H NMR-based metabonomic analysis of urine appears to be a promising, non-invasive approach to investigate and monitor pediatric nephrourological diseases.
authors.affiliation: Department of Toxicology, University of Cagliari, Italy. latzori@unica.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Atzori
authors.forename: Roberto
authors.initials: R
authors.lastname: Antonucci
authors.forename: Luigi
authors.initials: L
authors.lastname: Barberini
authors.forename: Emanuela
authors.initials: E
authors.lastname: Locci
authors.forename: Flaminia
authors.initials: F
authors.lastname: Cesare Marincola
authors.forename: Paola
authors.initials: P
authors.lastname: Scano
authors.forename: Patrizia
authors.initials: P
authors.lastname: Cortesi
authors.forename: Rino
authors.initials: R
authors.lastname: Agostiniani
authors.forename: Aalim
authors.initials: A
authors.lastname: Weljie
authors.forename: Adolfo
authors.initials: A
authors.lastname: Lai
authors.forename: Vassilios
authors.initials: V
authors.lastname: Fanos
mesh_terms: D015415:Biomarkers / Q000652:urine*; D002648:Child; D016002:Discriminant Analysis; D006801:Humans; D007558:Italy; D007668:Kidney / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D016482:Urinalysis / Q000379:methods*; D014570:Urologic Diseases / Q000652:urine*
chemical_list: D015415:Biomarkers