Proteomic analysis of Alternaria alternata (Fr.) Keissler responds to COS fumigation.
Carbonyl sulfide (COS) is a new fumigant which has been a potential alternative to methyl bromide and phosphine in many applications. In this study, we investigated the fungitoxicity of COS towards the pathogen of pear black spot disease Alternaria alternata (Fr.) Keissler (A. alternata). Moreover, proteomic analysis and RT-PCR was performed and our results showed that during the fumigation, the regulation of 21 proteins in protein expression and mRNA accumulation levels is involved, which respond to growth inhibition caused by COS. These results provide new clues for the mechanism of the fungitoxicity of COS.
Liu
Tao
T
Chinese Academy of Inspection and Quarantine, NO 241, Huixinxijie, Chaoyang district, Beijing 100029, PR China.
Li
Li
L
Qu
Haixia
H
Zhan
Guoping
G
Liu
Bo
B
Wang
Yuejin
Y
D000528:Alternaria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005651:Fumigation; D005656:Fungal Proteins / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013461:Sulfur Oxides / Q000633:toxicity*
D005656:Fungal Proteins; D005659:Fungicides, Industrial; D013461:Sulfur Oxides; C010063:carbonyl sulfide