title: Osteoblast-specific factor 2 expression in prostate cancer-associated stroma: identification through microarray technology.
abstract: OBJECTIVE
  To better understand the gene expression patterns in tumor-associated stroma, laser-capture-microdissections from clinical specimens were analyzed by genome-wide-expression microarray technology. The epithelial-stromal interaction plays a critical role in prostate development, reactive changes, and tumorigenesis. Diverse microarray technologies have been used to characterize the molecular changes in prostate cancer. Even though these gene expression studies are compromised by the heterogeneity of the tumor, as well as by the difficulty associated with collecting appropriate counterparts to represent normal prostate cells, the gene array data from tumors have shown promising results. Currently, little is known about the tumor-associated stromal gene expression profile in prostate cancer.
  
  
  METHODS
  Matching benign and malignant epithelial cell-related stroma cells were subjected to microarray platforms.
  
  
  RESULTS
  The prostatatic stroma expressed several osteogenic molecules. In particular, one of the genes, OSF2, was upregulated in tumor-associated stroma compared with benign epithelial cell associated stroma, which was further validated by immunohistochemical examination.
  
  
  CONCLUSIONS
  These data show that the combination of laser capture dissection with computational enhancement of epithelial and stromal microarray data is a useful tool to assess gene expression changes in prostate cancer stroma.
authors.affiliation: Center for Prostate Disease Research, Department of Surgery and United States Military Cancer Institute, Uniformed Service University of the Health Science, Bethesda, Maryland, USA. bfurusato@cpdr.org
authors.forename: Bungo
authors.initials: B
authors.lastname: Furusato
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Tsunoda
authors.forename: Syed
authors.initials: S
authors.lastname: Shaheduzzaman
authors.forename: Martin E
authors.initials: ME
authors.lastname: Nau
authors.forename: Maryanne
authors.initials: M
authors.lastname: Vahey
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Petrovics
authors.forename: David G
authors.initials: DG
authors.lastname: McLeod
authors.forename: Seiji
authors.initials: S
authors.lastname: Naito
authors.forename: Senji
authors.initials: S
authors.lastname: Shirasawa
authors.forename: Shiv
authors.initials: S
authors.lastname: Srivastava
authors.forename: Isabell A
authors.initials: IA
authors.lastname: Sesterhenn
mesh_terms: D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human