Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus.
The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium. Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.
Bajwa
Paramjit Kaur
PK
School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Arora
Daljit Singh
DS
D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D046508:Culture Techniques; D042845:Laccase / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000096:biosynthesis*; D020075:Phanerochaete / Q000201:enzymology* / Q000254:growth & development; D055492:Polyporus / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase