title: Site-specific labeling of 'second generation' annexin V with 99mTc(CO)3 for improved imaging of apoptosis in vivo.
abstract: In this study 'second generation' AnxV was specifically labeled with (99m)Tc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged 'second generation' AnxV labeled with (99m)Tc(CO)(3) in comparison to (99m)Tc-HYNIC-cys-AnxV and (99m)Tc(CO)(3)-DTPA-cys-AnxV.
authors.affiliation: Department of Nuclear Medicine, University Hospital Gasthuisberg Leuven, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Marijke
authors.initials: M
authors.lastname: De Saint-Hubert
authors.forename: Felix M
authors.initials: FM
authors.lastname: Mottaghy
authors.forename: Kathleen
authors.initials: K
authors.lastname: Vunckx
authors.forename: Johan
authors.initials: J
authors.lastname: Nuyts
authors.forename: Humphrey
authors.initials: H
authors.lastname: Fonge
authors.forename: Kristof
authors.initials: K
authors.lastname: Prinsen
authors.forename: Sigrid
authors.initials: S
authors.lastname: Stroobants
authors.forename: Luc
authors.initials: L
authors.lastname: Mortelmans
authors.forename: Niko
authors.initials: N
authors.lastname: Deckers
authors.forename: Leo
authors.initials: L
authors.lastname: Hofstra
authors.forename: Chris P M
authors.initials: CP
authors.lastname: Reutelingsperger
authors.forename: Alfons
authors.initials: A
authors.lastname: Verbruggen
authors.forename: Dirk
authors.initials: D
authors.lastname: Rattat
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000737:chemistry; D017209:Apoptosis; D022781:Hepatocytes / Q000166:cytology; D051379:Mice; D013667:Technetium / Q000737:chemistry; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D017304:Annexin A5; D013667:Technetium