title: Biosynthesis of acteoside in cultured cells of Olea europaea.
abstract: Five phenylethanoid glycosides (acteoside, isoacteoside, beta-oxoacteoside, beta-hydroxyacteoside, and salidroside) were isolated from a cell suspension culture of Olea europaea. We examined the biosynthesis of acteoside in olive cell cultures by using feeding experiments with stable isotope labeled precursors. The hydroxytyrosol moiety of acteoside is biosynthesized from tyrosine through dopamine, whereas the caffeoyl moiety of acteoside is biosynthesized from phenylalanine via a cinnamate pathway. Dopamine is incorporated into acteoside through oxidation to the corresponding aldehyde, reduction to the alcohol, and then beta-glycosylation.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saimaru
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Orihara
mesh_terms: D002478:Cells, Cultured; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry*; D031658:Olea; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols