title: Modulation of p53 gene expression in the intertidal copepod Tigriopus japonicus exposed to alkylphenols.
abstract: The copepod, Tigriopus japonicus has been recognized as an excellent marine model species for ecotoxicological studies. The present study cloned and characterized the p53 gene of this copepod and studied its expression pattern. We discovered that p53 expression patterns varied among different developmental stages of the copepod, having the highest expression in the adult. Such variation was possibly associated with the molting cycle. By using real-time RT-PCR, we further investigated the modulatory pattern of the p53 gene in the copepod after exposure to three alkylphenols (i.e. nonylphenol, octylphenol, and bisphenol A) which are known as endocrine disruption chemicals (EDCs). The results showed that the three alkylphenols significantly induced p53 gene expression in the copepod, indicating the involvement of p53 in such stress-responses. Thus, the copepod p53 gene provides one of the stress-response biomarkers for exposure to EDC-like compounds.
authors.affiliation: Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Dae-Sik
authors.initials: DS
authors.lastname: Hwang
authors.forename: Jin-Seon
authors.initials: JS
authors.lastname: Lee
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Han
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Gyung Soo
authors.initials: GS
authors.lastname: Park
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D015415:Biomarkers / Q000378:metabolism; D033342:Copepoda / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D016158:Genes, p53 / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015415:Biomarkers; D010636:Phenols; D016159:Tumor Suppressor Protein p53; D014874:Water Pollutants, Chemical; C474055:octylphenol; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds