Nanoscale resolution, multicomponent biomolecular arrays generated by aligned printing with parylene peel-off.
We present "Print-and-Peel", a high-throughput method to generate multicomponent biomolecular arrays with sub-100 nm nanoscale feature width. An inkjet printer is first aligned to a parylene template containing nanoscale openings. After printing, the parylene is peeled off to reveal uniformly patterned nanoscale features, despite the imperfect morphologies of the original inkjet spots. We further patterned combinatorial nanoarrays by performing a second print-run superimposed over the first, thereby extending the multiplexing capability of the technique.
Tan
Christine P
CP
Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, USA.
Cipriany
Benjamin R
BR
Lin
David M
DM
Craighead
Harold G
HG
D000327:Adsorption; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D049329:Nanostructures; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D014992:Xylenes / Q000737:chemistry*
D005353:Fibronectins; D011108:Polymers; D014992:Xylenes; C011055:parylene