title: Nucleotide variability of Tahyna virus (Bunyaviridae, Orthobunyavirus) small (S) and medium (M) genomic segments in field strains differing in biological properties.
abstract: Tahyna virus (TAHV), a mosquito-borne bunyavirus (California group), is frequently associated with inapparent or influenza-like (Valtice fever) infections in humans, rarely leading to atypical pneumonia or meningitis. Field TAHV strains exhibit a high variability in their biological properties with respect to virulence for laboratory mouse, temperature-sensitivity or character of plaques in cell culture. In consideration of the variations in the antigenic properties TAHV and its potential genetic variability, we analyzed complete nucleotide sequences of the small (S) and medium (M) genomic segments of field TAHV strains with different combinations of phenotypic markers. S segment was highly conservative in all analyzed TAHV strains. Within the M segment, the highest variability was observed in the G(C) gene encoding viral envelope protein and to a less extent also in the NSm gene. However, 5' and 3' non-coding regions of M segment, as well as in G(N) gene exhibited highly conservative pattern, indicating its functional importance, but minor or no role in the determination of biological properties of TAHV field strains.
authors.affiliation: Faculty of Science, University of South Bohemia, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic.
authors.forename: Patrik
authors.initials: P
authors.lastname: Kilian
authors.forename: Daniel
authors.initials: D
authors.lastname: Růzek
authors.forename: Vlasta
authors.initials: V
authors.lastname: Danielová
authors.forename: Václav
authors.initials: V
authors.lastname: Hypsa
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D016000:Cluster Analysis; D017124:Conserved Sequence; D002141:Encephalitis Virus, California / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004670:Encephalitis, California / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014759:Viral Envelope Proteins / Q000235:genetics; D010948:Viral Plaque Assay; D014774:Virulence
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014759:Viral Envelope Proteins