The monitoring of progress in apoptosis of liver cells in bile duct-ligated rats.
OBJECTIVE
We aimed to determine the progress of lipid peroxidation and ultrastructural changes established in the rat liver after acute bile duct ligation.


METHODS
Groups A1, B1, C1 and D1 were the controls of groups A2, B2, C2 and D2, which represented the 1st, 3rd, 5th and 8th days after bile duct ligation. Serum bilirubin and malondialdehyde, liver malondialdehyde and reduced glutathione levels, and inducible nitric oxide synthase expression were determined. Liver tissue was examined with light and electron microscopy.


RESULTS
Serum bilirubin increased progressively. Serum and liver malondialdehyde and inducible nitric oxide synthase expression reached a peak level at day 3, reduced at the 5th day and continued at a constant rate. Reduced glutathione decreased progressively. Ductal proliferation increased progressively to a plateau at day 5. The marked electron microscopic changes were detected at day 3 (B2) and continued constantly.


CONCLUSIONS
The first five days after acute bile duct ligation are the most critical.
Dirlik
Musa
M
Department of General Surgery, Mersin University, School of Medicine, Mersin. musa.dirlik@yahoo.com
Canbaz
Hakan
H
Düşmez Apa
Duygu
D
Cağlikülekçi
Mehmet
M
Yaylak
Faik
F
Balli
Ebru
E
Tamer
Lülüfer
L
Kanik
Arzu
A
Aydin
Süha
S
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001652:Bile Ducts; D001663:Bilirubin / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D041781:Jaundice, Obstructive / Q000378:metabolism* / Q000473:pathology*; D008026:Ligation; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008854:Microscopy, Electron; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D005978:Glutathione; D001663:Bilirubin