title: [Recent developments in preparation of monolithic columns and their applications in proteomic analysis].
abstract: Monolithic column is a continuous unitary porous structure prepared by in-situ polymerization or consolidation inside the column tubing. Monolithic columns have been attracted great attention due to their simple preparation procedure, high permeability and high mass transfer rate. Big progress on the technology and methods for the preparation of monolithic columns and their applications to the highly efficient separation and pretreatment of biological samples have been made in last decade. This review summarizes the developments in the preparation of the monolithic columns and their applications to the proteomic analysis during the last five years.
authors.affiliation: National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. hanfazou@dicp.ac.cn
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Minghuo
authors.initials: M
authors.lastname: Wu
authors.forename: Fangjun
authors.initials: F
authors.lastname: Wang
authors.forename: Renan
authors.initials: R
authors.lastname: Wu
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
mesh_terms: D002845:Chromatography / Q000379:methods*; D040901:Proteomics