[Effect of Paenibacillus polymyxa lectin on cytokine status of animals].
OBJECTIVE
To study effect of lectin L II of Paenibacillus polymyxa 1460 on cytokine status of mice during modeling of infections caused by Staphylococcus, Escherichia and Yersinia.


METHODS
Lectin LII of P. polymyxa 1460, bacterial cultures Staphylococcus aureus 209-P, Escherichia coli O1, and Yersinia enterocolitica 12 were used. Mice were inoculated intraperitoneally with 0.2 ml of suspension of bacterial culture grown during 24 hours (5,000 m.c./ml). Lectin (0.4 mcg/ml) in dose 0.2 ml was administered 24 h before and 1 h after inoculation. Serum samplesfrom sacrificed animals were obtained 1, 6, and 24 hours after inoculation and concentrations of IL-1, IL-6, and TNF-alpha were measured in them using optical density values.


RESULTS
It was established that level of TNF-alpha in serum decreased during staphylococcal infection, whereas levels of IL-1 and IL-6 decreased during all modeled infections. Ability for correction of cytokine balance in organism of experimental animals by administration of lectin 24 h before and 1 h after inoculation with bacteria was demonstrated.


CONCLUSIONS
Presented studies testify to the effect of bacterial lectin on cytokine-production activity of macrophages during phagocytosis of bacteria and infectious process.
Gorel'nikova
E A
EA
Tikhomirova
E I
EI
Karpunina
L V
LV
D000818:Animals; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D037102:Lectins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D056507:Paenibacillus / Q000276:immunology*; D010587:Phagocytosis; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D015009:Yersinia Infections / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000276:immunology
D016207:Cytokines; D037102:Lectins