title: Gongronella sp induces overproduction of laccase in Panus rudis.
abstract: Laccase is usually produced via chemical induction and is also synthesized by hosts in interaction with the typical bio-control genus Trichoderma. In this study, we found that a newly isolated non-laccase-producing fungus, Gongronella sp. W5, could induce overproduction of laccase in Panus rudis. The enzyme activity, 148,200 U l(-1), was 25 times higher than the activity obtained from a chemical induction using copper/o -toluidine as inducers. A new laccase isozyme from the interaction of P. rudis and G. W5 was purified and characterized. A further test showed that some pH resistant metabolites secreted by G. W5 acted as signals to induce P. rudis laccase. Laccase is also highly expressed by Trametes sp. AH28-2 in interaction with Trichoderma sp. ZH1. However, no laccase activity was observed from the cross-over interactions of P. rudis -Trichoderma sp. ZH1 or Trametes sp. AH28-2-G. W5.
authors.affiliation: School of Life Sciences and Biotechnology Center, Anhui University, Hefei, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wei
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Hong
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D018920:Coculture Techniques; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D042845:Laccase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D009090:Mucorales / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D020072:Polyporales / Q000201:enzymology*; D055454:Trametes / Q000378:metabolism; D014242:Trichoderma / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D007527:Isoenzymes; D042845:Laccase