title: Serum profile in preeclampsia and intra-uterine growth restriction revealed by iTRAQ technology.
abstract: In order to identify new protein markers modified in placental diseases, high-throughput analysis of proteins in the plasma of pregnant women was carried out for normal and pathological pregnancies (Preeclampsia and/or Intra-Uterine Growth Restriction) using iTRAQ technology. We could identify 166 proteins that were modified (p<0.05) and the technique used allowed the detection of previously undetected factors, such as various members of the SERPINA clade. The modifications of two proteins (C reactive protein and antichymotrypsin, SERPINA3) were validated on individual samples. Complement and coagulation cascades proteins were significantly enriched among modified protein clusters in the case of intra-uterine growth restriction (p<2.6.10(-11)). Several proteins were specifically enriched in isolated preeclampsia and depleted when preeclampsia was complicated by intra-uterine growth restriction. These findings suggest that the growth restricted foeto-placental unit is able to moderate some changes in maternal plasma composition. Overall, the use of iTRAQ technology, for the first time on this subject, enabled us to provide a new list of proteins modified in placental diseases, among which proteins expressed at a low level that were not accessible by other methods.
authors.affiliation: INSERM, U567, Institut Cochin, Team 21 Genomics and Epigenetics of Infertility and Placental Diseases, Paris, France.
authors.forename: Jana
authors.initials: J
authors.lastname: Auer
authors.forename: Luc
authors.initials: L
authors.lastname: Camoin
authors.forename: François
authors.initials: F
authors.lastname: Guillonneau
authors.forename: Virginie
authors.initials: V
authors.lastname: Rigourd
authors.forename: Sonia T
authors.initials: ST
authors.lastname: Chelbi
authors.forename: Marjorie
authors.initials: M
authors.lastname: Leduc
authors.forename: Jérôme
authors.initials: J
authors.lastname: Laparre
authors.forename: Thérèse-Marie
authors.initials: TM
authors.lastname: Mignot
authors.forename: Daniel
authors.initials: D
authors.lastname: Vaiman
mesh_terms: D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis; D011247:Pregnancy; D015843:Serpins / Q000097:blood*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; C516148:SERPINA3 protein, human; D015843:Serpins; D002097:C-Reactive Protein