title: Effect of hemolysis on insulin determination by the Beckman Coulter Unicell DXI 800 immunoassay analyzer.
abstract: OBJECTIVE
  To evaluate the effect of hemolysis on the measurement of insulin using the Beckman Coulter Unicell DXI 800 immunoassay analyzer.
  
  
  METHODS
  Insulin concentrations were measured using the Beckman Coulter Unicell DXI 800. Interference studies were conducted by mixing plasma of known insulin concentrations with hemolysates of increasing hemoglobin concentration. Hemolysates were prepared by osmotic shock. A change of >10% from baseline results was taken as evidence of significant interference.
  
  
  RESULTS
  Hemolysates mediated a concentration-dependent reduction in insulin concentration.
  
  
  CONCLUSIONS
  A concentration-dependent negative bias in the measurement of insulin is associated with increasing degrees of hemolysis on the Beckman Coulter Unicell DXI 800.
authors.affiliation: Southampton University Hospitals NHS Trust, Clinical Pathology Care Group, Division of Diagnostics & Therapy, Clinical Biochemistry, Level D, South Block, Mailpoint 6, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom. paul.cook@suht.swest.nhs.uk
authors.forename: Paul R
authors.initials: PR
authors.lastname: Cook
authors.forename: Christine
authors.initials: C
authors.lastname: Glenn
authors.forename: Annie
authors.initials: A
authors.lastname: Armston
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D001793:Blood Preservation / Q000009:adverse effects; D001800:Blood Specimen Collection / Q000379:methods; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007328:Insulin / Q000032:analysis* / Q000097:blood; D009994:Osmolar Concentration
chemical_list: D006454:Hemoglobins; D007328:Insulin