title: Templating membrane assembly, structure, and dynamics using engineered interfaces.
abstract: The physical and chemical properties of biological membranes are intimately linked to their bounding aqueous interfaces. Supported phospholipid bilayers, obtained by surface-assisted rupture, fusion, and spreading of vesicular microphases, offer a unique opportunity, because engineering the substrate allows manipulation of one of the two bilayer interfaces as well. Here, we review a collection of recent efforts, which illustrates deliberate substrate-membrane coupling using structured surfaces exhibiting chemical and topographic patterns. Vesicle fusion on chemically patterned substrates results in co-existing lipid phases, which reflect the underlying pattern of surface energy and wettability. These co-existing bilayer/monolayer morphologies are useful both for fundamental biophysical studies (e.g., studies of membrane asymmetry) as well as for applied work, such as synthesizing large-scale arrays of bilayers or living cells. The use of patterned, static surfaces provides new models to design complex membrane topographies and curvatures. Dynamic switchable-topography surfaces and sacrificial trehalose based-substrates reveal abilities to dynamically introduce membrane curvature and change the nature of the membrane-substrate interface. Taken together, these studies illustrate the importance of controlling interfaces in devising model membrane platforms for fundamental biophysical studies and bioanalytical devices.
authors.affiliation: Department of Applied Science, College of Engineering, University of California, Davis, CA 95616, USA.
authors.forename: Ann E
authors.initials: AE
authors.lastname: Oliver
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008954:Models, Biological; D008956:Models, Chemical; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids