[Isolation identification and serological analysis on varicella-zoster virus from an outbreak in Shanghai in 2007].
OBJECTIVE
To make serological analysis and identify the etiology of an outbreak of chickenpox in Zhabei District of Shanghai in 2007.


METHODS
IgM and IgG of the paired serums were detected by ELISA. The collected herpes were inoculated into MRC-5 cells to get VZV isolations and identify them by VZV standard serums. A 268bp fragment was amplified by specific PCR primers.


RESULTS
Among the five paired serums, two of the acute phase serums were VZV IgM positive, one was VZV IgG positive, and two were equivocal for anti-VZV IgG. All of the convalescent serums were VZV IgG positive, and four of them were 4 times higher in antibody titer than those of acute phase serums. Viruses were isolated from all of the 5 herpes, and then identified by VZV standard serum. The electrophoresis result of PCR products showed the single specific strap.


CONCLUSIONS
This outbreak was caused by VZV virus. 47.4% of the patients were inoculated in postnatal or domestic VZV vaccines in China 3 years after delivery. The effectiveness of VZV vaccine has been certified. It is necessary to consider the immunization strategy of VZV vaccine.
Lu
Jing
J
Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China.
Li
Chong-Shan
CS
Hu
Jia-Yu
JY
D000914:Antibodies, Viral / Q000097:blood; D002644:Chickenpox / Q000453:epidemiology / Q000276:immunology* / Q000821:virology; D002648:Child; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M