title: Growth and transcriptional effect of dietary 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio).
abstract: In this study, zebrafish (Danio rerio) were fed food dosed with pure PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) congener or a blank from 20 to 60 day post-hatch (dph). At 38 dph, half of the fish were sampled for body size measurements and gene expression analyzes (CYP1A1, VTG, TTR, D1, and TSH-beta). At 60 dph, body size was measured again for all fish remaining. Whole-fish histology was performed and the PBDE levels in fish were determined. PBDE treated fish was significantly smaller at 38 dph but not at 60 dph. No apparent histopathological effect was observed. In the PBDE treated fish, there was a weak induction of CYP1A1 mRNA transcription, but not of the other genes. The tissue levels of PBDE-47 were comparable to that found in other wild fish reported in the literature, indicating that our exposure level was ecologically relevant.
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung, Taiwan, ROC.
authors.forename: Te-Hao
authors.initials: TH
authors.lastname: Chen
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Cheng
authors.forename: Jing-O
authors.initials: JO
authors.lastname: Cheng
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Chou
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Hsiao
authors.forename: Fung-Chi
authors.initials: FC
authors.lastname: Ko
mesh_terms: D000821:Animal Feed; D000818:Animals; D049628:Body Size / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism / Q000633:toxicity*; D008018:Life Cycle Stages / Q000187:drug effects* / Q000502:physiology; D008136:Longevity / Q000187:drug effects; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D018675:Toxicity Tests; D014158:Transcription, Genetic / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D055768:Halogenated Diphenyl Ethers; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether; D019363:Cytochrome P-450 CYP1A1