Sequence polymorphism and PCR-restriction fragment length polymorphism analysis of the flagellin gene of Burkholderia pseudomallei.
Four flagellin allelic types (I to IV) of Burkholderia pseudomallei were identified based on their sequence variation and restriction fragment length polymorphism (RFLP) analysis of the amplified flagellin gene. Flagellin allelic type I was the most predominantly (75.0%) found among the 100 clinical isolates of B. pseudomallei investigated in this study.
Tay
Sun Tee
ST
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. tayst@um.edu.my
Cheah
Pui Ching
PC
Puthucheary
Savithiri D
SD
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D005408:Flagellin / Q000235:genetics*; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D005408:Flagellin