title: Determination of acid dissociation constants of compounds active at neuronal nicotinic acetylcholine receptors by means of electrophoretic and potentiometric techniques.
abstract: The dissociation constants of epiboxidine 2 and a series of bases active at neuronal nicotinic acetylcholine receptors were determined by means of potentiometric and electrophoretic methods, which gave values in good agreement. Although showing different features, the two techniques are complementary for dissociation constant determinations. The choice of the most suitable method is guided by the available amount of sample, its purity, and the time needed for the analysis. The experimental values were compared with the predictions obtained with ACD/pK(a) DB software.
authors.affiliation: Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy. gabriella.roda@unimi.it
authors.forename: Gabriella
authors.initials: G
authors.lastname: Roda
authors.forename: Clelia
authors.initials: C
authors.lastname: Dallanoce
authors.forename: Giovanni
authors.initials: G
authors.lastname: Grazioso
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Liberti
authors.forename: Marco
authors.initials: M
authors.lastname: De Amici
mesh_terms: D002021:Buffers; D004345:Drug Industry; D019075:Electrophoresis, Capillary; D007555:Isoxazoles / Q000737:chemistry; D007700:Kinetics; D018722:Nicotinic Agonists / Q000737:chemistry*; D011199:Potentiometry; D011978:Receptors, Nicotinic / Q000187:drug effects*; D012995:Solubility; D012997:Solvents
chemical_list: D002021:Buffers; D007555:Isoxazoles; D018722:Nicotinic Agonists; D011978:Receptors, Nicotinic; D012997:Solvents; C106388:epiboxidine