title: Quantitation of fentanyl in blood and urine using gas chromatography-mass spectrometry (GC-MS).
abstract: Fentanyl is a potent, short-acting synthetic opioid analgesic. Fentanyl is measured in blood and urine following mixed-mode solid phase extraction. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. The final extracts are reconstituted in methanol and analyzed using selected ion monitoring gas chromatography-mass spectrometry.
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Goldberger
authors.forename: Chris W
authors.initials: CW
authors.lastname: Chronister
authors.forename: Michele L
authors.initials: ML
authors.lastname: Merves
mesh_terms: D005283:Fentanyl / Q000097:blood* / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D005283:Fentanyl