title: [The role of endoscopic ultrasound-guided transesophageal fine needle aspiration and immunocytochemistry assesment of the patients with suspected lung cancer and negative bronchoscopic biopsies].
abstract: BACKGROUND
  Endoscopic ultrasound-(EUS) guided fine needle aspiration (FNA) allows the assessment of the posterior mediastinum, as well as the diagnosis and staging of lung cancer patients. The purpose of this feasibility study was to assess the importance of EUS-FNA combined with cytology and immunocytochemistry for patients with suspected lung cancer and negative bronchoscopic biopsies.
  
  
  METHODS
  Our study included 20 consecutive patients assessed at the Research Center of Gastroenterology and Hepatology Craiova, University of Medicine and Pharmacy Craiova. The patients were initially examined by chest X-ray, computer tomography scans and bronchoscopy, without a tissue confirmation of malignancy.
  
  
  CONCLUSIONS
  Of the 20 patients included in our study without a tissue confirmation of malignancy, 16 patients had a positive EUS-FNA for malignancy. For 11 patients the samples were obtained from the mediastinal lymphnodes, and for 4 cases directly from the primary mediastinal tumor, some of the obtained samples being included in paraffin to obtain cell blocks. The cell blocks allowed us to accomplish imunocytochemistry for two purposes: to establish the epithelial and mesenchimal fenotype of the malignant cells, as well as the origin of the identified atypical cells.
  
  
  CONCLUSIONS
  EUS-FNA combined with cytology, is an excellent minimal invasive technique, highly accurate for the assessment of lung cancer, showing not only the tumoral and lymph node invasion, but also offering the ideal alternative for surgical staging. Association of immunocytochemistry determined an increase in the accuracy of the method, as well as the confirmation of a tissue diagnosis of malignancy.
authors.affiliation: Centrul de Cercetare în Gastroenterologie şi Hepatologie Craiova, Universitatea de Medicină şi Farmacie din Craiova, România.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Ioncică
authors.forename: Carmen
authors.initials: C
authors.lastname: Popescu
authors.forename: Anca
authors.initials: A
authors.lastname: Maloş
authors.forename: Lucian
authors.initials: L
authors.lastname: Ioncică
authors.forename: Monalisa
authors.initials: M
authors.lastname: Filip
authors.forename: Sevastiţa
authors.initials: S
authors.lastname: Iordache
authors.forename: Larisa
authors.initials: L
authors.lastname: Săndulescu
authors.forename: Dan Ionuţ
authors.initials: DI
authors.lastname: Gheonea
authors.forename: Tudorel
authors.initials: T
authors.lastname: Ciurea
authors.forename: Daniela
authors.initials: D
authors.lastname: Neagoe
authors.forename: Adrian
authors.initials: A
authors.lastname: Săftoiu
mesh_terms: D044963:Biopsy, Fine-Needle; D019160:Endosonography / Q000295:instrumentation* / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000473:pathology*; D008198:Lymph Nodes / Q000000981:diagnostic imaging / Q000473:pathology; D009367:Neoplasm Staging; D012680:Sensitivity and Specificity