title: Molecular and experimental evidence refuse the life cycle  of Proctoeces lintoni (Fellodistomidae) in Chile.
abstract: The mussel Perumitylus purpuratus has been described as the first intermediate host for the digenea Proctoeces lintoni (Fellodistomidae) in the Chilean coast. The study of more than 3000 specimens of P. purpuratus, taken off northern Chile revealed the absence of sporocysts. Experimental infection of mussels with eggs obtained from the known host for P. lintoni was unsuccessful. We analyze the V4 region of the SSU rRNA of living sporocysts and cercariae obtained from P. purpuratus from central Chile in order to confirm the proposed life cycle for this digenea. Our results demonstrated that sporocysts and cercariae obtained from P. purpuratus do not belong to P. lintoni but to an undescribed digenea from the Chilean coast.
authors.affiliation: Facultad de Recursos del Mar, Instituto de Investigaciones Oceanológicas, Universidad Antofagasta, P.O. Box 170, Antofagasta, Chile. meoliva@uantof.cl
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Oliva
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Valdivia
authors.forename: Leyla
authors.initials: L
authors.lastname: Cárdenas
authors.forename: Mario
authors.initials: M
authors.lastname: George-Nascimento
authors.forename: Karen
authors.initials: K
authors.lastname: Gonzalez
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Guiñez
authors.forename: Diego
authors.initials: D
authors.lastname: Cuello
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology*; D002677:Chile; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S