[Novel diagnostic kit on the basis of stained polymeric carriers for epidemiologic surveillance in natural foci of Crimean-Congo hemorrhagic fever].
OBJECTIVE
To study diagnostic value of developed antigenic polymeric diagnostic kit for epidemiologic surveillance in natural foci of Crimean-Congo hemorrhagic fever (CCHF) in South federal district.


METHODS
Novel antigenic diagnostic kit on the basis of polymeric microspheres for reaction of volume agglomeration was developed. The kit is designed for detection of virus-specific antibodies in human serum and in serum of agricultural animals.


RESULTS
Laboratory and field trials of the kit showed its high diagnostic potency, it was included in methodical recommendations "Organization and accomplishment of measures against Crimean-Congo hemorrhagic fever on the territory of its natural foci in Russia". Use of antigenic polymeric kit for epidemiological surveillance allows for more complete and systemic understanding of CCHF epidemic process.


CONCLUSIONS
At present, the diagnostic kit is successfully used, alongside with ELISA and PCR, on different levels of epidemiologic surveillance for CCHF in Rostov region.
Verkina
L M
LM
Pichurina
N L
NL
Lomov
Iu M
IuM
Narkevich
A N
AN
Terent'eva
A N
AN
Karbyshev
G L
GL
Vodianitskaia
S Iu
SIu
Moskvitina
E A
EA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D008863:Microspheres; D011108:Polymers / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D012426:Russia / Q000453:epidemiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011108:Polymers; D011933:Reagent Kits, Diagnostic