title: Stakelama pacifica gen. nov., sp. nov., a new member of the family Sphingomonadaceae isolated from the Pacific Ocean.
abstract: A Gram reaction-negative, weakly motile, non-spore-forming, rod-shaped, aerobic bacterium designated strain JLT832(T) was isolated from surface water of the central Pacific Ocean and formed yellow colonies on rich organic (RO) medium. The strain was oxidase-negative and catalase-positive. Acid was produced from mannitol, glucose, sucrose, lactose, sorbitol, maltose, (+)-trehalose and d-fructose. No acid was produced from d-(+)-xylose. The major cellular fatty acids of strain JLT832(T) were C(18 : 1)ω7c, C(14 : 0) 2-OH and C(16 : 0). The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone-10 and spermidine were present as the major quinone and polyamine, respectively. The genomic DNA G+C content of strain JLT832(T) was 66.0±0.5mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate formed a tight branch within the family Sphingomonadaceae but was clearly separate from established genera in this family. The sequence similarities between the new isolate and type strains of established genera ranged from 90.5 to 94.9 %. Based on these data, strain JLT832(T) constitutes a novel genus and species, for which the name Stakelama pacifica gen. nov., sp. nov. is proposed. The type strain of Stakelama pacifica is JLT832(T) (=CGMCC 1.7294(T) =LMG 24686(T)).
authors.affiliation: State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.|State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Li-Kai
authors.initials: LK
authors.lastname: Hao
authors.affiliation: Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Xun
authors.initials: X
authors.lastname: Du
authors.affiliation: State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine