title: Quantitation of benzodiazepines in blood and urine using gas chromatography-mass spectrometry (GC-MS).
abstract: The benzodiazepine assay utilizes gas chromatography-mass spectrometry (GC-MS) for the analysis of diazepam, nordiazepam, oxazepam, temazepam, lorazepam, alpha-hydroxyalprazolam, and alpha-hydroxytriazolam in blood and urine. A separate assay is employed for the analysis of alprazolam. Prior to solid phase extraction, urine specimens are subjected to enzyme hydrolysis. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted by mixed-mode solid phase extraction. The benzodiazepine extracts are derivatized with N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSFTA) producing tert-butyldimethyl silyl derivatives; the alprazolam extracts are reconstituted in methanol without derivatization. The final extracts are then analyzed using selected ion monitoring GC-MS.
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Goldberger
authors.forename: Chris W
authors.initials: CW
authors.lastname: Chronister
authors.forename: Michele L
authors.initials: ML
authors.lastname: Merves
mesh_terms: D000525:Alprazolam / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry / Q000652:urine; D001569:Benzodiazepines / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D003975:Diazepam / Q000097:blood / Q000737:chemistry / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008140:Lorazepam / Q000097:blood / Q000737:chemistry / Q000652:urine; D003708:Nordazepam / Q000097:blood / Q000737:chemistry / Q000652:urine; D010076:Oxazepam / Q000097:blood / Q000737:chemistry / Q000652:urine; D052616:Solid Phase Extraction; D014229:Triazolam / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry / Q000652:urine
chemical_list: D001569:Benzodiazepines; D014229:Triazolam; C032318:alpha-hydroxyalprazolam; C028638:alpha-hydroxytriazolam; D003708:Nordazepam; D010076:Oxazepam; D008140:Lorazepam; D003975:Diazepam; D000525:Alprazolam