title: Expression of osteonectin/secreted protein acidic and rich in cysteine and matrix metalloproteinases in ameloblastoma.
abstract: BACKGROUND
  Ameloblastoma is the most common clinically-significant epithelial odontogenic tumor, and is considered a benign but locally-aggressive tumor of the craniofacial region. Osteonectin/secreted protein acidic and rich in cysteine (SPARC) is induced in response to a number of biological processes such as tumor growth and metastasis, whereas matrix metalloproteinases (MMPs) degrade the extracellular matrix and participate in various biological processes including tumor invasion and metastasis. We hypothesize that SPARC acts with MMPs for the local invasiveness of ameloblastoma. The aim of this study was to examine the association of SPARC with MMP-1, MMP-2, and MMP-9 in ameloblastoma.
  
  
  METHODS
  Immunohistochemical expression of SPARC, MMP-1, MMP-2, and MMP-9 as well as co-expression of SPARC and MMP-9 were examined in a cohort of 23 cases of ameloblastoma.
  
  
  RESULTS
  SPARC, MMP-1, -2, and -9 were detected in the cytoplasm of the ameloblastic-like columnar cells and stellate-reticulum-like cells as well as in the stromal tissues of fibroblasts and endothelial cells of our cohort of ameloblastoma patients. Furthermore, co-expression of SPARC and MMP-9 were found in 23 cases of ameloblastoma. This may be the first study to demonstrate that the expression level of SPARC was statistically correlated with MMP-9 but not with MMP-1 or -2 in ameloblastoma.
  
  
  CONCLUSIONS
  Our results suggest a putative association between SPARC and MMPs (especially MMP-9) in ameloblastoma to regulate tumor invasion.
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ling-Chang
authors.initials: LC
authors.lastname: Shen
authors.forename: Yuk-Kwan
authors.initials: YK
authors.lastname: Chen
authors.forename: Shue-Sang
authors.initials: SS
authors.lastname: Hsue
authors.forename: Shyh-Yu
authors.initials: SY
authors.lastname: Shaw
mesh_terms: D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000201:enzymology / Q000473:pathology*; D015331:Cohort Studies; D042783:Endothelial Cells / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D053505:Matrix Metalloproteinases, Secreted / Q000032:analysis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D015676:Osteonectin / Q000032:analysis*; D055815:Young Adult
chemical_list: D015676:Osteonectin; D053505:Matrix Metalloproteinases, Secreted; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1