Calcium-permeable AMPA receptors mediate glutamatergic signaling in neural precursor cells of the postnatal olfactory bulb.
Neural precursor cells (NPCs) in the mammalian olfactory bulb give rise to local inhibitory neurons that integrate into existing circuitry throughout adult life. However, the functional properties of neurotransmitter receptors expressed by NPCs are not well understood. In this study, we use patch-clamp recording and calcium imaging to explore the properties of glutamate receptors expressed by NPCs in the olfactory bulb subependymal layer. We find that calcium-permeable AMPA receptors (AMPARs) are the major receptor type underlying glutamatergic signaling in olfactory bulb NPCs. We also show that when transmitter uptake is reduced, glutamate spillover from distant nerve terminals in the olfactory bulb can activate nonsynaptic NPC AMPARs and generate increases in intracellular calcium. Together, these results suggest that Ca(2+) influx via AMPARs may contribute to calcium-dependent processes that govern NPC differentiation and maturation.
Darcy
Daniel P
DP
Department of Neuroscience, University of California, San Diego School of Medicine, La Jolla, California 92093, USA.
Isaacson
Jeffry S
JS
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism* / Q000502:physiology*; D002465:Cell Movement; D004594:Electrophysiology; D018698:Glutamic Acid / Q000502:physiology*; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D013569:Synapses / Q000502:physiology
D018091:Receptors, AMPA; D018698:Glutamic Acid; C104722:glutamate receptor ionotropic, AMPA 2; D002118:Calcium