title: Proteomic analysis of porcine saliva.
abstract: Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS-PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.
authors.affiliation: Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Gutiérrez
authors.forename: I
authors.initials: I
authors.lastname: Miller
authors.forename: K
authors.initials: K
authors.lastname: Hummel
authors.forename: K
authors.initials: K
authors.lastname: Nöbauer
authors.forename: S
authors.initials: S
authors.lastname: Martínez-Subiela
authors.forename: E
authors.initials: E
authors.lastname: Razzazi-Fazeli
authors.forename: M
authors.initials: M
authors.lastname: Gemeiner
authors.forename: J J
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008297:Male; D013058:Mass Spectrometry / Q000662:veterinary; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D020543:Proteome; D012471:Salivary Proteins and Peptides