title: The prevalence of antibodies to adenovirus serotype 5 in an adult Indian population and implications for adenovirus vector vaccines.
abstract: In vivo gene delivery using human adenovirus serotype 5 (AdV5) vectors is being explored for vaccination purposes. The presence of anti-AdV5 antibodies in human serum arising from natural exposure to AdV5 can interfere potentially with and compromise the efficacy of rAdV5-based vaccine vectors. In this report, a collection of 114 sera from healthy adult Indian blood donors was analyzed for the presence of anti-AdV5 antibodies, using an AdV5 vector encoding the green fluorescent protein (GFP) to monitor the presence of anti-AdV5 neutralizing antibodies in human sera based on their ability to block virus entry into HeLa cells which express the Coxsackievirus-and-Adenovirus Receptor (CAR). In this assay all samples tested were positive for anti-AdV5 antibodies, with titers varying over a very wide range. It was also observed that these antibodies facilitated the uptake of the reporter AdV5 vector into the monocytic cell line U937 which does not express CAR, but expresses Fc receptors (FcRs) instead. These observations have implications for rAdV5-based vaccine development. J. Med. Virol. 82:407-414, 2010. (c) 2010 Wiley-Liss, Inc.
authors.affiliation: International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Pilankatta
authors.forename: Tanu
authors.initials: T
authors.lastname: Chawla
authors.forename: Navin
authors.initials: N
authors.lastname: Khanna
authors.forename: Sathyamangalam
authors.initials: S
authors.lastname: Swaminathan
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000276:immunology; D000260:Adenoviruses, Human / Q000276:immunology*; D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D019067:Antibody-Dependent Enhancement; D001782:Blood Donors; D002460:Cell Line; D005260:Female; D005822:Genetic Vectors / Q000276:immunology*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests / Q000379:methods; D016036:Seroepidemiologic Studies; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral