[Study of effect of thermolabile enterotoxin of Enterobacter cloacae on expression of cytokine genes in mice].
OBJECTIVE
To study the role of production of several cytokines by macrophages and neutrophils in experiment.


METHODS
Production of cytokines--IL-2, 4, 6, 10, 12, 15 as well as interferon gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), transforming growth factor beta (TGF-beta) by macrophages and neutrophils during experiment on mice inoculated with enterotoxigenic strain of Enterobacter cloacae was measured. Expression of cytokines was determined by reverse transcription PCR and dot-hybridization on the basis of specially synthesized nucleotide sequences.


RESULTS
In macrophages of experimental animals inoculated with enterotoxigenic strain of E. cloacae increased production of TGF-beta, IFN-gamma and decreased level of TNF-alpha were revealed. Transcription of mRNAs of IL-15 and IL-6 was detected in macrophages and neutrophils of experimental animals at 24 h after inoculation. Transcription of mRNAs of IL-2, IL-4 and IL-10 was detected neither in macrophages nor in neutrophils.


CONCLUSIONS
Thermolabile enterotoxin of E. cloacae renders negative effect on IFN-gamma, promotes enhanced expression of TGF-beta and suppresses production of IL-2, IL-4, IL- 10. Absence of expression of key cytokines suggests that effect of thermolabile enterotoxin of Enterobacter bacteria excludes it from cytokine regulation of immune response during infectious process.
Akhtarieva
A A
AA
Dolgushin
I I
II
Gabidullin
Z G
ZG
Gabidullin
Iu Z
IuZ
Kamalova
A A
AA
Akhmadeev
R M
RM
D000818:Animals; D016207:Cytokines / Q000235:genetics*; D016972:Enterobacter cloacae / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D005786:Gene Expression Regulation; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis
D016207:Cytokines; D004768:Enterotoxins; D012333:RNA, Messenger