title: Potential mechanisms underlying the acute lung dysfunction and bacterial extrapulmonary dissemination during Burkholderia cenocepacia respiratory infection.
abstract: BACKGROUND
  Burkholderia cenocepacia, an opportunistic pathogen that causes lung infections in cystic fibrosis (CF) patients, is associated with rapid and usually fatal lung deterioration due to necrotizing pneumonia and sepsis, a condition known as cepacia syndrome. The key bacterial determinants associated with this poor clinical outcome in CF patients are not clear. In this study, the cytotoxicity and procoagulant activity of B. cenocepacia from the ET-12 lineage, that has been linked to the cepacia syndrome, and four clinical isolates recovered from CF patients with mild clinical courses were analysed in both in vitro and in vivo assays.
  
  
  METHODS
  B. cenocepacia-infected BEAS-2B epithelial respiratory cells were used to investigate the bacterial cytotoxicity assessed by the flow cytometric detection of cell staining with propidium iodide. Bacteria-induced procoagulant activity in cell cultures was assessed by a colorimetric assay and by the flow cytometric detection of tissue factor (TF)-bearing microparticles in cell culture supernatants. Bronchoalveolar lavage fluids (BALF) from intratracheally infected mice were assessed for bacterial proinflammatory and procoagulant activities as well as for bacterial cytotoxicity, by the detection of released lactate dehydrogenase.
  
  
  RESULTS
  ET-12 was significantly more cytotoxic to cell cultures but clinical isolates Cl-2, Cl-3 and Cl-4 exhibited also a cytotoxic profile. ET-12 and CI-2 were similarly able to generate a TF-dependent procoagulant environment in cell culture supernatant and to enhance the release of TF-bearing microparticles from infected cells. In the in vivo assay, all bacterial isolates disseminated from the mice lungs, but Cl-2 and Cl-4 exhibited the highest rates of recovery from mice livers. Interestingly, Cl-2 and Cl-4, together with ET-12, exhibited the highest cytotoxicity. All bacteria were similarly capable of generating a procoagulant and inflammatory environment in animal lungs.
  
  
  CONCLUSIONS
  B. cenocepacia were shown to exhibit cytotoxic and procoagulant activities potentially implicated in bacterial dissemination into the circulation and acute pulmonary decline detected in susceptible CF patients. Improved understanding of the mechanisms accounting for B. cenocepacia-induced clinical decline has the potential to indicate novel therapeutic strategies to be included in the care B. cenocepacia-infected patients.
authors.affiliation: Departamento de Microbiologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Brazil.
authors.forename: Luiz G
authors.initials: LG
authors.lastname: Cunha
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Assis
authors.forename: Gloria-Beatriz
authors.initials: GB
authors.lastname: Machado
authors.forename: Ana P
authors.initials: AP
authors.lastname: Assef
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Marques
authors.forename: Robson S
authors.initials: RS
authors.lastname: Leão
authors.forename: Alessandra M
authors.initials: AM
authors.lastname: Saliba
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Plotkowski
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000382:microbiology* / Q000503:physiopathology*; D000818:Animals; D019121:Burkholderia Infections / Q000150:complications / Q000382:microbiology / Q000503:physiopathology; D016956:Burkholderia cepacia / Q000145:classification* / Q000502:physiology*; D005260:Female; D008168:Lung / Q000382:microbiology*; D051379:Mice; D012141:Respiratory Tract Infections / Q000382:microbiology* / Q000503:physiopathology*; D013045:Species Specificity