Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis.
BACKGROUND
Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots.


OBJECTIVE
To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana.


METHODS
The chromatographic separation was performed on a Shiseido CapCell PAK C18 analytical column efficiently using gradient elution with acetonitrile and water.


RESULTS
All calibration curves showed excellent linear regressions (R(2) > 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples.


CONCLUSIONS
The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.
Lee
Ki Yong
KY
College of Pharmacy and Research institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Korea.
Cho
Young Woong
YW
Park
Junghyun
J
Lee
Dong Young
DY
Kim
Seung Hyun
SH
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025341:Principal Component Analysis; D031946:Pulsatilla / Q000737:chemistry*; D011786:Quality Control; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis*
D012503:Saponins; D014315:Triterpenes