Glucocorticoid suppresses steroidogenesis in rat progenitor Leydig cells.
Glucocorticoid (GC) inhibits testosterone production in adult Leydig cells by the glucocorticoid receptor (GR). However, whether GC affects the development of Leydig cells is unclear. The goal of the present study is to investigate the effects of GC on steroidogenesis of rat progenitor Leydig cells (PLCs) in vitro. Dexamethasone (DEX) inhibited androsterone (AO) production in PLCs. The GR antagonist RU38486 reversed the DEX-induced inhibition of AO, whereas the mineralocorticoid receptor antagonist RU28318 did not. RU38486 also reversed DEX-induced reductions in steady-state mRNA levels of steroidogenic acute regulatory protein (Star) and 3β-hydroxysteroid dehydrogenase 1 (Hsd3b1). Steroidogenic acute regulatory protein (StAR) protein expression and 3β-hydroxysteroid dehydrogenase (3βHSD) enzyme activity were affected similarly. These results show that GCs inhibit steroidogenesis of PLCs by suppression of StAR and 3βHSD via a GR-mediated mechanism.
Xiao
Ye-Chen
YC
Population Council, New York, NY 10065, USA.
Huang
Ya-Dong
YD
Hardy
Dianne O
DO
Li
Xiao-Kun
XK
Ge
Ren-Shan
RS
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects*; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors*; D013148:Spironolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects; D000098682:Steroidogenic Acute Regulatory Protein
D000728:Androgens; D005938:Glucocorticoids; D000451:Mineralocorticoid Receptor Antagonists; D010750:Phosphoproteins; D011965:Receptors, Glucocorticoid; D000098682:Steroidogenic Acute Regulatory Protein; D013148:Spironolactone; D015735:Mifepristone; C041006:RU 28318; D003907:Dexamethasone; D015096:3-Hydroxysteroid Dehydrogenases