Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization.
We have investigated surface-enhanced plasmon resonance detection of DNA hybridization. Surface enhancement was based on the excitation of localized surface plasmon using subwavelength nanogratings, at a 300 nm period, coated with 24-mer ssDNA oligonucleotide, while optical signatures of DNA were amplified at the same time by gold nanoparticles conjugated with complementary ssDNA strands. When using nanoparticles of different sizes, maximum sensitivity enhancement, of more than 18 times, was obtained with nanoparticles of 20 nm diameter. This enhancement is mainly due to nanoparticle-associated signal amplification. Additional surface enhancement boosted the detection sensitivity by 57%. We have also confirmed the sensitivity enhancement to be linearly related to nanoparticle volume.
Moon
Seyoung
S
Program for Nanomedical Science and Technology, Yonsei University, Seoul, Korea, 120-749.
Kim
Dong Jun
DJ
Kim
Kyujung
K
Kim
Donghyun
D
Lee
Hosub
H
Lee
Kangtaek
K
Haam
Seungjoo
S
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D053770:Nanowires / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D055620:Optical Phenomena; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000706:statistics & numerical data
D004247:DNA