Detection of 2,4,6-trinitrotoluene-utilizing anaerobic bacteria by 15N and 13C incorporation.
2,4,6-Trinitrotoluene ((15)N or (13)C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [(15)N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [(13)C]TNT cultures and was related to Lysobacter taiwanensis.
Gallagher
Erin M
EM
Department of Environmental Sciences, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
Young
Lily Y
LY
McGuinness
Lora M
LM
Kerkhof
Lee J
LJ
D001421:Bacteria, Anaerobic / Q000302:isolation & purification* / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments; D053528:Lysobacter / Q000235:genetics; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014303:Trinitrotoluene / Q000378:metabolism*
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009587:Nitrogen Isotopes; D012336:RNA, Ribosomal, 16S; D014303:Trinitrotoluene