Neuroprotective effects of postconditioning on lipid peroxidation and apoptosis after focal cerebral ischemia/reperfusion injury in rats.
OBJECTIVE
Apoptosis after cerebral ischemia/reperfusion (I/R) injury leads to the process of cell death. The deal therapeutic approach would target the apoptosis after I/R. Ischemic postconditioning is a recently discovered neuroprotective strategy that involves the application of brief mechanical reperfusion with a specific algorithm at the onset of reperfusion following an ischemic period.


METHODS
Transient MCAo was performed on male SD (275+/-25 g) rats with intraluminal thread insertion for 2 hrs. Rats (n:36) were treated with postconditioning after 60 minutes of occlusion. The postconditioning algorithm was 30 secs of brief reperfusion followed by 30 secs of MCAo and this cycle was repeated 3 times at the onset of reperfusion.


RESULTS
After I/R injury, % change of the malonyldialdehyde (MDA) levels in the cortex, which is an index of lipid peroxidation, was found significantly higher in the I/R group. On the other hand postconditioning upregulated Bcl-2 and Bax translocation to the mitochondria, and caspase-3 activity and also reduced oxidative stress levels.


CONCLUSIONS
These findings indicated this neuroprotective effect is most likely achieved by antiapoptotic mechanisms through caspase pathways.
Abas
Faruk
F
Uludag University, Faculty of Medicine, Department of Neurosurgery, Bursa, Turkey.
Alkan
Tulin
T
Goren
Bulent
B
Taskapilioglu
Ozgur
O
Sarandol
Emre
E
Tolunay
Sahsene
S
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D053148:Caspase 3 / Q000378:metabolism; D007839:Functional Laterality; D019194:Ischemic Preconditioning; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018696:Neuroprotective Agents; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D018696:Neuroprotective Agents; D051028:bcl-2-Associated X Protein; D053148:Caspase 3