MiR-150 promotes gastric cancer proliferation by negatively regulating the pro-apoptotic gene EGR2.
Accumulating evidence suggests small non-coding RNAs (microRNAs) play important roles in human cancer progression. In the present study, we found miR-150 was overexpressed in gastric cancer cell lines and tissues. Ectopic expression of miR-150 promoted tumorigenesis and proliferation of gastric cancer cells. Luciferase reporter assay demonstrated that EGR2 was a direct target of miR-150. Collectively, our study demonstrated that overexpression of miR-150 in gastric cancer could promote proliferation and growth of cancer cells at least partially through directly targeting the tumor-suppressor EGR2, suggesting a potential strategy for the development of miRNA-based treatment of gastric cancer.
Wu
Qiong
Q
Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Jin
Haifeng
H
Yang
Zhiping
Z
Luo
Guanhong
G
Lu
Yuanyuan
Y
Li
Kai
K
Ren
Gui
G
Su
Tao
T
Pan
Yan
Y
Feng
Bin
B
Xue
Zengfu
Z
Wang
Xin
X
Fan
Daiming
D
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051767:Early Growth Response Protein 2 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
D051767:Early Growth Response Protein 2; D035683:MicroRNAs; C497326:EGR2 protein, human; C533097:MIR150, human