title: [Autologous mesenchymal stem cells culture from adipose tissue for treatment of facial rhytids].
abstract: OBJECTIVE
  To test the effect of mesenchymal stem cells (MSC) from adipose tissue on the dermal filling for nasolabial rhytids correction.
  
  
  METHODS
  50 cc of infraumbilical fat and 20 ml of peripheral blood were harvested to isolate MSC and autologous plasma from 15 female volunteers, respectively. The volunteers were grouped in according to the following strategies of intra-dermal injection: Group (I) only hyaluronic acid; Group (II) only MSC; Group (III) MSC combined with hyaluronic acid. For this qualitative and prospective study photographic monitoring was done monthly.
  
  
  RESULTS
  In the group (I) we observed an immediate effect of filling; in the group (II) the effect of filling was reached after approximately two months. In the group (III) filling occurred more efficiently and progressively, probably due to the combination of the short and long-term effects generated by the hyaluronic acid and MSC, respectively.
  
  
  CONCLUSIONS
  MSC when combined with hyaluronic acid were able to fill in deep folds, with progressive improvement of skin tone and decreasing lines of expression.
authors.affiliation: Departamento de Cirurgia Geral, Serviço de Cirurgia Plástica, Hospital Universitário Clementino Fraga Filho, UFRJ, RJ, Brasil. cesar@claudiodasilva.com
authors.forename: César
authors.initials: C
authors.lastname: Claudio-da-Silva
authors.forename: Leandra Santos
authors.initials: LS
authors.lastname: Baptista
authors.forename: Rosana Bizon Vieira
authors.initials: RB
authors.lastname: Carias
authors.forename: Hélio da Cunha
authors.initials: Hda C
authors.lastname: Menezes Neto
authors.forename: Radovan
authors.initials: R
authors.lastname: Borojevic
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid; D045164:Mesenchymal Stem Cell Transplantation; D008875:Middle Aged; D015361:Rhytidoplasty / Q000379:methods*
chemical_list: D006820:Hyaluronic Acid