[Constituents from Alternanthera philoxeroides and their antitumor activity].
OBJECTIVE
To investigate antitumor constituents from n-butyl alcohol extract of Alternanthera philoxeroides.


METHODS
The constituents were isolated with silica gel, gel permeation chromatography, and purified by HPLC. Their structures were elucidated by spectroscopy and acid hydrolysis. The antitumor effects of extracts and isolated compounds were tested by MTH method in vitro.


RESULTS
Seven compounds were isolated and elucidated as followings: oleanolic acid 3-O-beta-D-glucuronopyranoside (1), oleanolic acid 28-O-beta-D-glucopyranoside (2), oleanolic acid 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (3), chikusetsusaponin IV a methyl ester (4), hederagenin 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (5), 4,5-dihydroblumenol (6), 6S,7E,9R-6,9-di-hydroxymegastigma-4,7-dien-3-one-9-O-beta-D-glucopyranoside (7). Compound 1 showed significant inhibitory effect against Hela and L929 with inhibitive ratios 91.3% and 92.9% at 30 mg x L(-1), respectively.


CONCLUSIONS
Compounds 4, 5 and 7 were isolated from this genus for the first time. Compound 1 showed significant inhibitory effect against Hela and L929 at 30 mg x L(-1).
Fang
Jinbo
J
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Chen
Jiachun
J
Liu
Yanwen
Y
Duan
Hongquan
H
D027703:Amaranthaceae / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal