[Evaluation of 5 kinds of China-made enzyme immunoassay kits for antibody to hepatitis B virus e antigen detection].
OBJECTIVE
The aim of this study is to select a better one from five EIA kits made in China for the serological detection of anti-HBe in a national hepatitis B survey.


METHODS
Top 5 kinds of kits on the best seller list of China-made anti-HBe EIA detection were selected and each was detected the anti-HBe panel (66 positive and 88 negative) 5 times. The co/s ratio of each test was recorded and transformed by log(co/s+1). ICC and CV, the reliability indices and AUC, pAUC and Se(PR=e), the validity indices were calculated and compared between every two kits.


RESULTS
The indices of ICC and CV of the 5 kinds of kits arranged in the same order of A, E, C, D and B, from the best to the worst. For ICC, there were significantly different between every two of them (Bootstrap method, P < 0.05), except for between kit C and E. The magnitude ofAUCs were from 0.969 to 0.996; the orders of pAUC and Se(FPR=e) indices was C, E, D, A, B, and C, D, E, B, A respectively; both the indices of kit C were significantly higher than others (Bootstrap method, P < 0.05).


CONCLUSIONS
All the 5 kinds of kits are excellent and kit C is the best.
Wang
Feng
F
State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Ma
Jing-Chen
JC
Yu
Tao
T
D002681:China; D006509:Hepatitis B / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood* / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000032:analysis / Q000276:immunology*; D006515:Hepatitis B virus / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000191:economics / Q000592:standards
D006510:Hepatitis B Antibodies; D006513:Hepatitis B e Antigens; D011933:Reagent Kits, Diagnostic