Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens.
A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-alpha and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 microg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.
Na
Ye Seul
YS
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea.
Kim
Woo Jung
WJ
Kim
Sung Min
SM
Park
Jae Kweon
JK
Lee
Sae Mi
SM
Kim
Sung Oog
SO
Synytsya
Andriy
A
Park
Yong Il
YI
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000460:Chlorophyta / Q000737:chemistry*; D004396:Coloring Agents; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015850:Interleukin-6 / Q000096:biosynthesis; D007723:Korea; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000276:Adjuvants, Immunologic; D004396:Coloring Agents; D016207:Cytokines; D015850:Interleukin-6; D009005:Monosaccharides; D011134:Polysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C022616:thiazolyl blue