title: Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications.
abstract: 2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77843, United States.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Wan
authors.forename: William K
authors.initials: WK
authors.lastname: Russell
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Pai
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
authors.forename: Wenshe
authors.initials: W
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007651:Keto Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*
chemical_list: D000596:Amino Acids; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D007651:Keto Acids