Influence of the hepatitis C virus 3'-untranslated region on IRES-dependent and cap-dependent translation initiation.
Translation of hepatitis C virus (HCV) genomic RNA is directed by an internal ribosome entry site (IRES) in the 5'-untranslated region (5'-UTR), and the HCV 3'-UTR enhances IRES activity. Since the HCV 3'-UTR has a unique structure among 3'-UTRs, we checked possible communication between the 5'- and the 3'-UTR of HCV during translation using chimeric reporter RNAs. We show that translation directed by the HCV IRES and by the HCV-like IRES of porcine teschovirus (PTV) which belongs to a quite distinct family of viruses (picornaviruses) or by the EMCV IRES is also enhanced by the HCV 3'-UTR or by a poly(A)-tail in different cell types.
Bung
C
C
Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University Giessen, Germany.
Bochkaeva
Z
Z
Terenin
I
I
Zinovkin
R
R
Shatsky
I N
IN
Niepmann
M
M
D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D045744:Cell Line, Tumor; D004686:Encephalomyocarditis virus / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009154:Mutation; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000235:genetics*; D012315:RNA Caps / Q000235:genetics*; D020871:RNA Stability; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D013552:Swine; D052700:Teschovirus / Q000235:genetics; D014162:Transfection
D020413:3' Untranslated Regions; D012315:RNA Caps; D012367:RNA, Viral; D008156:Luciferases