title: Characterization of hydrogen peroxide production by Duox in bronchial epithelial cells exposed to Pseudomonas aeruginosa.
abstract: Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H2O2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa.
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda 20852, USA.
authors.forename: Balázs
authors.initials: B
authors.lastname: Rada
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Leto
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D016956:Burkholderia cepacia / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D000074623:Dual Oxidases; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D000074623:Dual Oxidases; D019255:NADPH Oxidases; C431513:DUOX1 protein, human; D002118:Calcium