title: Immunosuppressive ultraviolet-A radiation inhibits the development of skin memory CD8 T cells.
abstract: Ultraviolet A (UVA) radiation can have dual affects on the immune system depending on dose. At doses of approximately 1.8 J cm(-2), UVA acts in an immunosuppressive manner, whilst at higher doses UVA can promote recovery and protection against UVB-induced immunosuppression in mice. We utilised a model of contact hypersensitivity (CHS) to investigate how different doses of UVA modulates CD8 T cell immunity against a hapten in vivo. Only 1.8 J cm(-2) UVA decreased the CHS response compared to unirradiated mice, but this did not correlate with an inhibition of primary effector CD8 T cells. A similar expansion of effector CD8 T cells in skin-draining lymph nodes and accumulation of IFN-gamma-producing CD8 T cells in the ear skin was observed between unirradiated and UVA-irradiated mice. However, dermal memory CD8 T cells examined 9 weeks post challenge showed decreased numbers in mice irradiated with 1.8 J cm(-2) UVA compared with unirradiated, 1.3 J cm(-2) and 3.4 J cm(-2) UVA-irradiated mice. Therefore, UVA does not inhibit the expansion, migration or IFN-gamma secretion of CD8 T cells during a primary immune response. However, exposure to immunosuppressive UVA causes a defect in CD8 T cell development that impairs the ability of cells to become long-term memory cells.
authors.affiliation: Discipline of Dermatology, Bosch Institute, Sydney Cancer Centre, The University of Sydney, NSW 2006, Australia.
authors.forename: Sabita
authors.initials: S
authors.lastname: Rana
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Rogers
authors.forename: Gary M
authors.initials: GM
authors.lastname: Halliday
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects*; D003877:Dermatitis, Contact / Q000276:immunology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007108:Immune Tolerance / Q000528:radiation effects*; D051379:Mice; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays