Gramella marina sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.
The taxonomic position of a novel Gram-stain-negative, aerobic, heterotrophic, gliding, yellow-orange-pigmented bacterium, isolated from the sea urchin Strongylocentrotus intermedius and designated strain KMM 6048(T), was established. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the family Flavobacteriaceae affiliated with recognized species of the genus Gramella, forming a distinct lineage within the genus. 16S rRNA gene sequence similarities between strain KMM 6048(T) and the type strains of species of the genus Gramella were 97.4-98.4 %. In line with representative members of the genus Gramella, strain KMM 6048(T) was oxidase- and catalase-positive, hydrolysed gelatin and starch, utilized carbohydrates and possessed a DNA G+C content of 40.0mol%. However, differentiating phenotypic traits and phylogenetic distinctiveness clearly indicated that the strain represented a novel species within the genus Gramella, for which the name Gramella marina sp. nov. is proposed; the type strain is KMM 6048(T) (=KCTC 12366(T)=LMG 25418(T)).
Nedashkovskaya
Olga I
OI
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Kim
Seung Bum
SB
Department of Microbiology, College of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
Bae
Kyung Sook
KS
Biological Resources Center, Korea Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D047269:Strongylocentrotus / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6