[Effect of PIH1D1 on the degradation of its binding protein SNF5].
OBJECTIVE
To explore the effects of PIH1D1 on its binding protein SNF5, a core subunit of the SWI/SNF chromatin remodeling complex.


METHODS
The degradation pathway of SNF5 was identified with protein synthesis inhibitor cycloheximide (CHX) and a potent proteasome inhibitor MG132, and then the PIH1D1 eukaryotic expression plasmid was transfected to explore its effect on the stability of SNF5.


RESULTS
HEK293T cells were effectively treated with CHX (optimal concentration: 400 microg/ml) and MG132 (optimal concentration: 20 mmol/L). The degradation of SNF5 was mediated by the proteasome pathway. PIH1D1 regulated the protein level of SNF5 by attenuating its proteasome degradation.


CONCLUSIONS
PIH1D1 may stabilize SNF5 by attenuating its proteasome degradation pathway.
Zhai
Niu
N
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing 100005, China.
Zhang
Ye
Y
Shen
Yu-fei
YF
D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D000071796:SMARCB1 Protein; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C555143:PIH1D1 protein, human; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D046988:Proteasome Endopeptidase Complex