Antibody microarray typing, a novel technique for Streptococcus pneumoniae serotyping.
The use of antibody-based miniaturized devices for microbiological applications is a field poorly investigated in the era of more developed molecular amplification techniques. A novel antibody microarray for Streptococcus pneumoniae serotyping was developed, by printing nanolitre volumes of pneumococcal serotype-specific antibodies on multi-well slides. This microarray, which showed high specificity when tested against reference and clinical S. pneumoniae isolates, can be applicable as a faster, cost-effective and accurate serotyping technique for pneumococcal epidemiological studies.
Marimon
J M
JM
Servicio de Microbiología, Hospital Donostia, San Sebastián, Spain. JOSEMARIA.MARIMONORTIZDEZ@osakidetza.net
Monasterio
A
A
Ercibengoa
M
M
Pascual
J
J
Prieto
I
I
Simón
L
L
Perez-Trallero
E
E
D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D002648:Child; D006801:Humans; D011008:Pneumococcal Infections / Q000382:microbiology*; D040081:Protein Array Analysis / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000191:economics / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors
D000907:Antibodies, Bacterial