Role of IL-4 in aversion induced by food allergy in mice.
To ascertain the role of IL-4 in aversion to antigen induced by food allergy, wild type and IL-4 deficient BALB/c mice were sensitized with ovalbumin and challenged orally with egg white. Sensitized wild type mice had increased production of IL-4 by spleen and mesenteric lymph node cells in vitro, higher levels of serum anti-ovalbumin IgE and IgG1, aversion to ingestion of the antigen and loss of body weight after continuous oral challenge. Intestinal changes in wild type sensitized mice included eosinophil infiltration and increased mucus production. The IL-4 deficiency impaired the development of food allergy and the aversion to antigen, suggesting the involvement of the antigen specific antibodies. When IL-4 deficient mice received serum from sensitized wild type donors, the aversion was restored. These results indicate that production of IL-4 and specific IgE/IgG1 antibodies correlate with aversion to antigen induced by food allergy in mice.
Dourado
Luana Pereira Antunes
LP
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Saldanha
Janaína Cláudia da Silva
JC
Gargiulo
Daniela Longo
DL
Noviello
Maria de Lourdes Meirelles
Mde L
Brant
Cláudia Caldeira
CC
Reis
Maria Letícia Costa
ML
Souza
Raphaela Mendes Fernandes de
RM
Faria
Ana Maria Caetano
AM
Souza
Danielle da Glória de
Dda G
Cara
Denise Carmona
DC
D000818:Animals; D001835:Body Weight; D002645:Chickens; D004063:Digestion; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000172:deficiency / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism
D007074:Immunoglobulin G; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin