title: Human papillomavirus is not associated with colorectal cancer in a large international study.
abstract: OBJECTIVE
  Recent publications have reported an association between colon cancer and human papillomaviruses (HPV), suggesting that HPV infection of the colonic mucosa may contribute to the development of colorectal cancer.
  
  
  METHODS
  The GP5+/GP6+ PCR reverse line blot method was used for detection of 37 types of human papillomavirus (HPV) in DNA from paraffin-embedded or frozen tissues from patients with colorectal cancer (n = 279) and normal adjacent tissue (n = 30) in three different study populations, including samples from the United States (n = 73), Israel (n = 106) and Spain (n = 100). Additionally, SPF10 PCR was run on all samples (n = 279) and the Innogenetics INNO-LiPA assay was performed on a subset of samples (n = 15).
  
  
  RESULTS
  All samples were negative for all types of HPV using both the GP5+/GP6+ PCR reverse line blot method and the SPF10 INNO-LiPA method.
  
  
  CONCLUSIONS
  We conclude that HPV types associated with malignant transformation do not meaningfully contribute to adenocarcinoma of the colon.
authors.affiliation: Department of Human Genetics, School of Medicine, University of Michigan, 109 Zina Pitcher, Ann Arbor, MI 48109-2200, USA.
authors.forename: Michele C
authors.initials: MC
authors.lastname: Gornick
authors.forename: Xavier
authors.initials: X
authors.lastname: Castellsague
authors.forename: Gloria
authors.initials: G
authors.lastname: Sanchez
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Giordano
authors.forename: Michelle
authors.initials: M
authors.lastname: Vinco
authors.forename: Joel K
authors.initials: JK
authors.lastname: Greenson
authors.forename: Gabriel
authors.initials: G
authors.lastname: Capella
authors.forename: Leon
authors.initials: L
authors.lastname: Raskin
authors.forename: Gad
authors.initials: G
authors.lastname: Rennert
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Gruber
authors.forename: Victor
authors.initials: V
authors.lastname: Moreno
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D016022:Case-Control Studies; D002472:Cell Transformation, Viral; D015179:Colorectal Neoplasms / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007557:Israel; D008297:Male; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D013030:Spain; D014481:United States
chemical_list: D004279:DNA, Viral