Inducing stroke in aged, hypertensive, diabetic rats.
Animal models of ischemic stroke often neglect comorbidities common in patients. This study shows the feasibility of inducing stroke by 2 h of thread occlusion of the middle cerebral artery in aged (56 week old) spontaneously hypertensive rats (SHRs) with both acute (2 weeks) and chronic (36 weeks) diabetes. After modifying the streptozotocin dosing regimen to ensure that old SHRs survived the induction of diabetes, few died after induction of stroke. Induction of stroke is feasible in rats with multiple comorbidities. Inclusion of such comorbid animals may improve translation from the research laboratory to the clinic.
Rewell
Sarah S J
SS
National Stroke Research Institute and University of Melbourne Department of Medicine, Austin Health, Heidelberg, Victoria, Australia.
Fernandez
John A
JA
Cox
Susan F
SF
Spratt
Neil J
NJ
Hogan
Lisa
L
Aleksoska
Elena
E
van Raay
Leena
L
Liberatore
Gabriel T
GT
Batchelor
Peter E
PE
Howells
David W
DW
D000818:Animals; D015897:Comorbidity; D003921:Diabetes Mellitus, Experimental / Q000453:epidemiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000503:physiopathology*; D051381:Rats; D011918:Rats, Inbred SHR; D020521:Stroke / Q000453:epidemiology / Q000401:mortality / Q000503:physiopathology*