Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques.
Here we describe a novel vaccine vector for expressing human immunodeficiency virus (HIV) antigens. We show that recombinant attenuated yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 Gag sequences can be used as a vector to generate SIV-specific CD8(+) T-cell responses in the rhesus macaque. Priming with recombinant BCG expressing SIV antigens increased the frequency of these SIV-specific CD8(+) T-cell responses after recombinant YF17D boosting. These recombinant YF17D-induced SIV-specific CD8(+) T cells secreted several cytokines, were largely effector memory T cells, and suppressed viral replication in CD4(+) T cells.
Bonaldo
Myrna C
MC
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Dr., Madison, WI 53711, USA.
Martins
Mauricio A
MA
Rudersdorf
Richard
R
Mudd
Philip A
PA
Sacha
Jonah B
JB
Piaskowski
Shari M
SM
Costa Neves
Patrícia C
PC
Veloso de Santana
Marlon G
MG
Vojnov
Lara
L
Capuano
Saverio
S
Rakasz
Eva G
EG
Wilson
Nancy A
NA
Fulkerson
John
J
Sadoff
Jerald C
JC
Watkins
David I
DI
Galler
Ricardo
R
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D008253:Macaca mulatta; D010446:Peptide Fragments / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D022341:Yellow Fever Vaccine / Q000276:immunology; D015005:Yellow fever virus / Q000235:genetics*
D015683:Gene Products, gag; D010446:Peptide Fragments; D018449:SAIDS Vaccines; D014614:Vaccines, Synthetic; D022341:Yellow Fever Vaccine