Isolation of beta-mannanase from Cocos nucifera Linn haustorium and its application in the depolymerization of beta-(1,4)-linked D-mannans.
Beta-mannanase was extracted from coconut (Cocos nucifera Linn) haustorium and purified through ammonium sulfate precipitation and sepharose 6B-lectin affinity chromatography. Coconut beta-mannanase is an acidic protein with a pI of 3.75. The molecular mass of coconut beta-mannanase (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was found to be 44 kDa and was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The optimum temperature and pH for enzyme activity was 70 degrees C and 5.2. The enzyme was used for the preparation of neutraceutical dietary supplement from galactomannans of guar gum and tender coconut kernel having a beta-(1,4)-linked D-mannose backbone. Depolymerized guar gum has 92% of oligosaccharides with a degree of polymerization of 3 and 7. Tender coconut kernel has a degree of polymerization of 9-39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize beta-(1,4)-linked D-mannose polysaccharides from most plant sources to produce prebiotics in a cost-effective technique.
Soumya
Rema S
RS
Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, India.
Abraham
Emilia T
ET
D003059:Cocos / Q000737:chemistry / Q000201:enzymology*; D019587:Dietary Supplements; D005685:Galactans / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D018514:Plant Structures; D011108:Polymers; D056692:Prebiotics; D012639:Seeds; D044902:beta-Mannosidase / Q000737:chemistry / Q000302:isolation & purification*
D005685:Galactans; D008351:Mannans; D010936:Plant Extracts; D053149:Plant Gums; D011108:Polymers; D056692:Prebiotics; C007894:guar gum; D044902:beta-Mannosidase