title: Discrimination of animal species using polymorphisms of the nuclear gene zinc finger protein 238.
abstract: We screened 3750 single exonic genes listed in the intronless genes in the eukaryotes (SEGE) database and performed bioinformatic analyses to identify candidate genes for new species-specific markers. A set of PCR primers for the conserved regions of ZNF238 was developed and used to amplify the 823 bp DNA fragment. We compared nucleotide variations of the PCR products among 20 species plus two subspecies of animals, which led to the identification of interspecies nucleotide variations. To establish a simple method for the analysis of species-specific DNA polymorphisms using ZNF238, we developed a PCR-RFLP method using HhaI and HpyCH4IV restriction enzymes for 13 species. For the remaining species, the direct sequencing of PCR products provided additional SNPs, enabling precise species classification. As a result, we report here that a new nuclear DNA marker, ZNF238, can be used to increase the accuracy of species identification among euteleostomi (bony vertebrates).
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Won
authors.initials: W
authors.lastname: Kim
authors.forename: Sunmi
authors.initials: S
authors.lastname: Kim
authors.forename: Hojun
authors.initials: H
authors.lastname: Choi
authors.forename: Nguyen Dinh
authors.initials: ND
authors.lastname: Truong
authors.forename: Le Minh
authors.initials: le M
authors.lastname: Thong
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Keun-Kyu
authors.initials: KK
authors.lastname: Park
authors.forename: Kunho
authors.initials: K
authors.lastname: Seo
authors.forename: Hang
authors.initials: H
authors.lastname: Lee
authors.forename: Bo-Sook
authors.initials: BS
authors.lastname: Kim
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Yoo
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D000835:Animals, Wild / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D019295:Computational Biology; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005784:Gene Amplification; D008322:Mammals / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D004247:DNA