title: Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) in diverse clinical specimens by the BD GeneOhm MRSA assay and comparison with culture.
abstract: The efficacy of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay was assessed by analyzing nasal swabs and swabs from other body sites for the presence of MRSA in a low-prevalence area. From 681 patients with a high risk for MRSA carriage, 1,601 specimens were collected and transported in Amies agar. After discordant analysis, the sensitivity, specificity, positive predictive value, and negative predictive value of the BD GeneOhm MRSA assay were 84.3%, 99.2%, 88.4%, and 98.9%, respectively, compared to culture.
authors.affiliation: Department of Medical Microbiology, Center for Laboratory Medicine, Luzerner Kantonsspital Luzern, 6000 Lucerne 16, Switzerland. klucke@imm.uzh.ch
authors.forename: Katja
authors.initials: K
authors.lastname: Lucke
authors.forename: Michael
authors.initials: M
authors.lastname: Hombach
authors.forename: Melanie
authors.initials: M
authors.lastname: Hug
authors.forename: Gaby E
authors.initials: GE
authors.lastname: Pfyffer
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009297:Nasal Mucosa / Q000382:microbiology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology