title: Optical label-free nanoplasmonic biosensing using a vertical-cavity surface-emitting laser and charge-coupled device.
abstract: We present a compact platform for biochemosensing based on the combination of a vertical-cavity surface-emitting laser (VCSEL) light source, microelectromechanical systems (MEMS)-based microoptics, a specially designed nanoplasmonic sensing chip, and charge-coupled device (CCD) detector. The platform does not require any spectral analyzer for signal evaluation, showing good promise for facile integration, neither does it use any microscope setup for the signal collection or imaging. The analytical capabilities of the developed biochemosensing platform are demonstrated by evaluation of the protein-substrate (biotinylated bovine serum albumin-gold) and the protein-protein (biotin-NeutrAvidin) binding kinetics, which is further compared to detection based on conventional optical extinction spectroscopy. The instrument is able to detect low femtomoles of adsorbed proteins with the limit of detection comparable to the state-of-the-art research and commercial optical label-free biochemosensors.
authors.affiliation: BioNano Systems Laboratory, MC2, Chalmers University of Technology, 412 96 Göteborg, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Hedsten
authors.forename: Jordi
authors.initials: J
authors.lastname: Fonollosa
authors.forename: Peter
authors.initials: P
authors.lastname: Enoksson
authors.forename: Peter
authors.initials: P
authors.lastname: Modh
authors.forename: Jörgen
authors.initials: J
authors.lastname: Bengtsson
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dmitriev
mesh_terms: D000327:Adsorption; D000818:Animals; D001360:Avidin / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D002417:Cattle; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D007834:Lasers; D008027:Light; D036103:Nanotechnology; D055620:Optical Phenomena; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: C093483:neutravidin; D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin; D006046:Gold