[Inhibitory role of SirT7 in the growth of P19 cell line].
OBJECTIVE
To study the regulatory role of SirT7, one of class histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19.


METHODS
We used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper.


RESULTS
Compared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line.


CONCLUSIONS
SirT7 play a dominant role in the grow inhibition of the P19 cells.
Lu
Jian-yi
JY
National Laboratory of Medical Molecular Biology, Institute of National Laboratory of Medical Molecular Biology, Beijing 100005, China.
Zhang
Ye
Y
Shen
Yu-fei
YF
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids / Q000235:genetics; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
C539736:Sirt7 protein, mouse; D037761:Sirtuins