High expression stability of microtubule affinity regulating kinase 3 (MARK3) makes it a reliable reference gene.
Difference in gene expressions is characteristic of the function of different cell types and those genes with low expression variance can be used as standards for quantitative gene expression studies. Microarray technology is used to study global gene expression within a cell; hence, represents a suitable source of data to mine for genes with low expression variance. The coefficient of variation (COV) of each gene was determined and a threshold of less than 0.1 COV was used to select stably expressed genes in each data set. Our results showed that microtubule affinity-regulating kinase 3 (MARK3) has the lowest COV in eight microarray datasets. In addition, the gene expression of housekeeping genes, which is very likely to be stably expressed, tends to fluctuate highly under different conditions, marking them as being less reliable for use as reference genes.
Chia
Ching-Yee
CY
Nanyang Girls' High School, Ministry of Education, Singapore.
Lim
Claudia W X
CW
Leong
Wan-Ting
WT
Ling
Maurice H T
MH
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000096:biosynthesis; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012269:Ribosomal Proteins / Q000096:biosynthesis
D012269:Ribosomal Proteins; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); C540215:Mark3 protein, mouse; D017346:Protein Serine-Threonine Kinases