Overexpression of stathmin1 in the diffuse type of gastric cancer and its roles in proliferation and migration of gastric cancer cells.
BACKGROUND
Stathmin1 is a microtubule-regulating protein that has an important role in the assembly and disassembly of the mitotic spindle. The roles of stathmin1 in carcinogenesis of various cancers, including prostate and breast cancer, have been explored. However, its expression and roles in gastric cancer have not yet been described.


METHODS
Stathmin1 expression in paraffin-embedded tissue sections from 226 patients was analysed by immunohistochemistry. Roles of stathmin1 were studied using a specific small interfering RNA (siRNA).


RESULTS
The expression of stathmin1 was positively correlated with lymph node metastasis, TNM stages and vascular invasion, and negatively with recurrence-free survival, in the diffuse type of gastric cancer. The median recurrence-free survival in patients with a negative and positive expression of stathmin1 was 17.0 and 7.0 months, respectively (P=0.009). When the expression of stathmin1 was knocked down using siRNA, the proliferation, migration and invasion of poorly differentiated gastric cancer cells in vitro were significantly inhibited. Moreover, stathmin1 siRNA transfection significantly slowed the growth of xenografts in nude mice.


CONCLUSIONS
These results suggest that stathmin1 can be a good prognostic factor for recurrence-free survival rate and is a therapeutic target in diffuse-type gastric cancer.
Jeon
T-Y
TY
Department of Surgery, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, South Korea.
Han
M-E
ME
Lee
Y-W
YW
Lee
Y-S
YS
Kim
G-H
GH
Song
G-A
GA
Hur
G-Y
GY
Kim
J-Y
JY
Kim
H-J
HJ
Yoon
S
S
Baek
S-Y
SY
Kim
B-S
BS
Kim
J-B
JB
Oh
S-O
SO
D000368:Aged; D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000494:pharmacology; D050777:Stathmin / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
D034741:RNA, Small Interfering; C493995:STMN1 protein, human; D050777:Stathmin