title: Endothelial nitric oxide synthase haplotypes associated with hypertension do not predispose to cardiac hypertrophy.
abstract: Left ventricular hypertrophy (LVH) is a complication that may result from chronic hypertension. While nitric oxide (NO) deficiency has been associated with LVH, inconsistent results have been reported with regards to the association of endothelial NO synthase (eNOS) polymorphisms and LVH in hypertensive patients. This study aims to assess whether eNOS haplotypes are associated with LVH in hypertensive patients. This study included 101 healthy controls and 173 hypertensive patients submitted to echocardiography examination. Genotypes for three eNOS polymorphisms were determined: a single-nucleotide polymorphism in the promoter region (T-786C) and in exon 7 (Glu298Asp), and variable number of tandem repeats in intron 4. We found no significant association between eNOS genotypes and hypertension or with LVH (all p > 0.05). However, while we found two eNOS haplotypes associated with variable risk of hypertension (all p < 0.05), we found no significant associations between eNOS haplotypes and LVH (all p > 0.05), even after adjustment in multiple linear regression analysis. These findings suggest that eNOS haplotypes that have been associated with variable susceptibility to hypertension were not associated with LVH in hypertensive patients. Further studies are necessary to examine whether other genes downstream may interact with eNOS polymorphisms and predispose to LVH in hypertensive patients.
authors.affiliation: Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, USP, Ribeirao Preto, Brazil.
authors.forename: Vivian
authors.initials: V
authors.lastname: Vasconcellos
authors.forename: Riccardo
authors.initials: R
authors.lastname: Lacchini
authors.forename: Anna L B
authors.initials: AL
authors.lastname: Jacob-Ferreira
authors.forename: Maria L
authors.initials: ML
authors.lastname: Sales
authors.forename: Maria C
authors.initials: MC
authors.lastname: Ferreira-Sae
authors.forename: Roberto
authors.initials: R
authors.lastname: Schreiber
authors.forename: Wilson
authors.initials: W
authors.lastname: Nadruz
authors.forename: Jose E
authors.initials: JE
authors.lastname: Tanus-Santos
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology; D001794:Blood Pressure / Q000502:physiology; D015992:Body Mass Index; D006332:Cardiomegaly / Q000235:genetics* / Q000473:pathology; D004452:Echocardiography; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006352:Heart Ventricles / Q000473:pathology; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012727:Sex Characteristics
chemical_list: D008055:Lipids; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III