Loss of heterozygosity of selected tumor suppressor genes in human testicular germ cell tumors.
Human testicular germ cell tumors (TGCTs) are histologically heterogenous neoplasms with a variable malignant potential. Two main groups of germ cell tumors occur in men: seminomas and nonseminomas. In the present study, a set of four tumor suppressor genes was investigated in testicular cancers. CDH1, APC, p53, and nm23-H1 genes were tested for loss of heterozygosity (LOH). Thirty-eight testicular germ cell tumors (17 seminomas and 21 nonseminomas) were analyzed by PCR using restriction fragment length polymorphism or the dinucleotide/tetranucleotide repeat polymorphism method. An allelic loss of p53 at exon 4 was detected in five nonseminomas, whereas LOH of p53 at intron 6 occurred in one of the seminoma and two of the nonseminoma samples. Allelic losses of the APC gene were present in three seminomas and one nonseminoma, whereas one seminoma and three nonseminomas showed LOH of CDH1. The analysis of allelic losses showed no common structural genetic alterations in tumor tissues, although a different pattern of LOH was observed between the two main histological groups of TGCTs.
Vladusić
Tomislav
T
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia. tvladusic@pbf.hr
Hrasćan
Reno
R
Vrhovac
Ivana
I
Kruslin
Bozo
B
Gamulin
Marija
M
Grgić
Mislav
M
Pećina-Slaus
Nives
N
Franekić Colić
Jasna
J
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics; D017491:Genes, APC; D016147:Genes, Tumor Suppressor; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013736:Testicular Neoplasms / Q000235:genetics*; D055815:Young Adult
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D054778:NM23 Nucleoside Diphosphate Kinases