Repeated cocaine administration increases cleaved poly(ADP-ribose) polymerase-1 expression in the rat dorsal striatum.
Poly(ADP-ribose) polymerase-1 (PARP-1) expression is associated with the endoplasmic reticulum (ER) stress response in neurons. In this study, the regulation of PARP-1 activation by repeated cocaine administration in the rat dorsal striatum was investigated in vivo. Our results demonstrated that repeated systemic injections of cocaine (20 mg/kg) once a day for 7 days increased cleaved PARP-1 expression. This increase was reduced by blocking dopamine D1, but not dopamine D2, receptors. The blocking of group I metabotropic glutamate receptors (mGluRs), mGluR1 subtype, and N-methyl-D-aspartate receptors also reduced cocaine-stimulated cleaved PARP-1 expression. Taken together, these findings suggest that PARP-1 activation is upregulated by repeated cocaine administration, and that interactions between dopamine D1 and glutamate receptors may be involved in this upregulation.
Yang
Ju Hwan
JH
Department of Biological Sciences, Pusan National University, 30 Jangjeon-dong, Kumjeong-gu, Biology Bldg., Room 311, Pusan 609-735, Republic of Korea.
Choe
Eun Sang
ES
D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000201:enzymology; D004789:Enzyme Activation; D008297:Male; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology
D000697:Central Nervous System Stimulants; D017447:Receptors, Dopamine D1; D018094:Receptors, Metabotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; C104077:metabotropic glutamate receptor type 1; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D003042:Cocaine