HPLC purification of adenoviral vectors.
Adenoviruses are attractive vectors for gene therapy where short-term transgene expression is required. In order to meet the clinical requirements of adenovirus for use beyond the laboratory, advanced methods are required for the purification and quantitation of recombinant adenoviral vectors (rAd). Chromatographic systems offer the advantages of linear scalability and reproducibility, and this method describes a laboratory-scale process based on liquid chromatography, which can be technically transferred and readily scaled-up according to the demands of the laboratory or clinic in which it will be used.
Eglon
Marc
M
Regenerative Medicine Institute, National University of Ireland, Galway, Ireland.
McGrath
Barry
B
O'Brien
Timothy
T
D000256:Adenoviridae / Q000302:isolation & purification*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005822:Genetic Vectors / Q000302:isolation & purification*; D006801:Humans