Plasma mediated collagen-I-coating of metal implant materials to improve biocompatibility.
This study describes the collagen-I coating of titanium and steel implants via cold low-pressure gas plasma treatment. To analyze the coatings in terms of biocompatibility osteoblast-like osteosarcoma cells and human leukocytes were cultivated on the metal surfaces. Two different implant materials were assessed (Ti6Al4V, X2CrNiMo18) and four different surface properties were evaluated: (a) plasma pretreated and collagen-I coated implant materials; (b) collagen-I dip-coated without plasma pretreatment; (c) plasma treated but not collagen-I coated; (d) standard implant materials served as control. The different coating characteristics were analyzed by scanning electron microscopy (SEM). For adhesion and viability tests calcein-AM staining of the cells and Alamar blue assays were performed. The quantitative analysis was conducted by computer assisted microfluorophotography and spectrometer measurements. SEM analysis revealed that stable collagen-I coatings could not be achieved on the dip-coated steel and titanium alloys. Only due to pretreatment with low-pressure gas plasma a robust deposition of collagen I on the surface could be achieved. The cell viability and cell attachment rate on the plasma pretreated, collagen coated surfaces was significantly (p < 0.017) increased compared to the non coated surfaces. Gas plasma treatment is a feasible method for the deposition of proteins on metal implant materials resulting in an improved biocompatibility in vitro. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
Hauser
Joerg
J
Department of Plastic Surgery, BG Trauma Center, Bergmannsheil University Hospital, Bochum, Germany. joerg.hauser@rub.de
Koeller
Manfred
M
Bensch
Sebastian
S
Halfmann
Helmut
H
Awakowicz
Peter
P
Steinau
Hans-Ulrich
HU
Esenwein
Stefan
S
D000497:Alloys; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007962:Leukocytes / Q000166:cytology; D008422:Materials Testing; D010078:Oxazines / Q000737:chemistry; D019736:Prostheses and Implants; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014966:Xanthenes / Q000737:chemistry
D000497:Alloys; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; D013193:Stainless Steel; C031462:titanium alloy (TiAl6V4); C085925:calcein AM; C005843:resazurin; D014025:Titanium