Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis.
Arabinomannan-containing glycolipids, relevant to the mycobacterial cell-wall component lipoarabinomannan, were synthesized by chemical methods. The glycolipids were presented with tri- and tetrasaccharide arabinomannans as the sugar portion and a double alkyl chain as the lyophilic portion. Following synthesis, systematic biological and biophysical studies were undertaken in order to identify the effects of the glycolipids during mycobacterium growth. The studies included mycobacterial growth, biofilm formation and motility assays. From the studies, it was observed that the synthetic glycolipid with higher arabinan residues inhibited the mycobacterial growth, lessened the biofilm formation and impaired the motility of mycobacteria. A surface plasmon resonance study involving the immobilized glycan surface and the mycobacterial crude lysates as analytes showed specificities of the interactions. Further, it was found that cell lysates from motile bacteria bound oligosaccharide with higher affinity than non-motile bacteria.
Naresh
Kottari
K
Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India.
Bharati
Binod Kumar
BK
Avaji
Prakash Gouda
PG
Jayaraman
Narayanaswamy
N
Chatterji
Dipankar
D
D018441:Biofilms / Q000187:drug effects; D006017:Glycolipids / Q000134:cerebrospinal fluid / Q000737:chemistry* / Q000494:pharmacology*; D008351:Mannans / Q000737:chemistry*; D009068:Movement / Q000187:drug effects*; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D020349:Surface Plasmon Resonance
D006017:Glycolipids; D008351:Mannans; C012189:arabinomannan