Characterization of genomic structures and expression profiles of three tandem repeats of a mouse double homeobox gene: Duxbl.
We identified and cloned a mouse double homeobox gene (Duxbl), which encodes two homeodomains. Duxbl gene, a tandem triplicate produces two major transcripts, Duxbl and Duxbl-s. The amino acid sequences of Duxbl homeodomains are most similar to those of human DUX4 protein, associated with facioscapulohumeral muscular dystrophy. In adult tissues, Duxbl is predominantly expressed in female reproductive organs and eyes, and slightly expressed in brain and testes. During gonad development, Duxbl is expressed from embryonic to adult stages and specifically expressed in oocytes and spermatogonia. During embryonic development, Duxbl is transcribed in limbs and tail. However, Duxbl proteins were only detected in trunk and limb muscles and in elongated myocytes and myotubes. In C2C12 muscle cell line, Duxbl expression pattern is similar to differentiated marker gene, Myogenin, increased in expression from 2 days onward in differentiating medium. We suggest that Duxbl proteins play regulatory roles during myogenesis and reproductive developments.
Wu
Shey-Lin
SL
Department of Neurology, Chang-Hua Christian Hospital, and Department of Bioindustry Technology, Da-Yeh University, Dacun, Changhua, Taiwan.
Tsai
Ming-Shiun
MS
Wong
Swee-Hee
SH
Hsieh-Li
Hsiu-Mei
HM
Tsai
Tz-Shiu
TS
Chang
Wei-Tang
WT
Huang
Shin-Ling
SL
Chiu
Chun-Ching
CC
Wang
Sue-Hong
SH
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology; D002454:Cell Differentiation; D015509:Developmental Biology / Q000379:methods; D005121:Extremities / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009136:Muscular Dystrophies / Q000235:genetics; D009865:Oocytes / Q000166:cytology; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics*
C553465:Duxbl protein, mouse; D018398:Homeodomain Proteins; D014157:Transcription Factors