[Recent advances and applications of capillary electrochromatography and pressurized capillary electrochromatography].
Capillary electrochromatography (CEC), in which electroosmotic flow (EOF) created from the electrical double layer is made to act as a pump to drive the mobile phase in a capillary column packed with micro-particulates or coated with stationary phase. Both neutral and charged species can be resolved by CEC. It has been demonstrated that the efficiency of a separation obtained by electroosmotic propulsion is superior to that obtained by pressure-driven flow (as is the case in HPLC). CEC combines the best features of CE and versatile selectivity and large sample capacity of HPLC, promising high efficiency, high resolution, high selectivity and high peak capacity. However, in practice, when CEC is used without pressure, often used on a commercial CE instrument, there are problems and difficulties associated with bubbles formation and column dry-out. These difficulties can be overcome by a pressurized CEC (pCEC) system, in which a supplementary pressure is applied to the column in addition to the EOF. In such a system, a pressure can be applied to the capillary column to suppress bubbles formation. Quantitative sample introduction in pCEC can be easily achieved through a rotary-type injector. Most importantly, it is amenable for a solvent gradient mode, similar to that in HPLC, by programming the composition of mobile phase. The article brings a comprehensive survey of recent development of CEC and pCEC, including the development of instrumentation, capillary columns and stationary phase as well as CEC and pCEC applications in life science, biotechnology, pharmaceutical analysis, food safety and environmental security. Prospects for CEC and pCEC development and application are also discussed.
Wu
Yi
Y
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhang
Xiaohui
X
Wei
Juan
J
Xue
Yunyun
Y
Bahatibieke
Marjan
M
Wang
Yan
Y
Yan
Chao
C
D001690:Biological Science Disciplines; D001709:Biotechnology; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D002626:Chemistry, Pharmaceutical; D004463:Ecology; D005524:Food Technology