Differentiation factors that influence neuronal markers expression in vitro from human amniotic epithelial cells.
The differentiation of neural cells from embryonic stem cells is influenced by several growth factors. Amniotic epithelial cells (AECs) share many of the same characteristics as embryonic stem cells, and therefore those factors may similarly affect the derivation of neural cells from AECs. In this study, we examined the differentiation of neural cells in vitro from AECs following AECs treatment with retinoic acid (RA), basic fibroblast growth factor (bFGF) as well as investigation of bFGF withdrawal on neuronal differentiation. We also studied whether blocking bone morphogenetic protein (BMP) signaling using its antagonist, noggin, affects the derivation of neuronal cells from AECs. The effects of serum on the rate of neural markers expression were also examined. Analysis of AECs derived neurons was performed at some neural markers expression level by immunocytochemistry. All cultures treated with noggin showed the higher levels of neural markers expression than noggin free cultures. Combined treatment with bFGF and RA showed the highest level of neural markers in all treatment groups with or without noggin. bFGF withdrawal did not promote expression of neural markers, while its maintenance increased the expression of these markers. Serum-free condition decreased the viability of cells but increased the rate of neural markers expression. These results show the capability of AECs to express neural cell markers and this ability is affected by some factors including serum, noggin, bFGF and RA.
Niknejad
Hassan
H
Nanomedicine and Tissue Engineering Research Center, Shaheed Beheshti Medical University, Tehran, Iran. niknejad@sbmu.ac.ir
Peirovi
Habibollah
H
Ahmadiani
Abolhassan
A
Ghanavi
Jalal
J
Jorjani
Masoumeh
M
D000650:Amnion / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D044967:Serum; D014212:Tretinoin / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism; D000099176:Noggin Protein
D015415:Biomarkers; D002352:Carrier Proteins; D004815:Epidermal Growth Factor; D016222:Fibroblast Growth Factor 2; D014212:Tretinoin; D014404:Tubulin; D000099176:Noggin Protein