[Genetic characteristic of wild-type measles viruses isolated in Ningbo from 2004 to 2008].
OBJECTIVE
To investigate the genetic characterizations and genotypes of measles viruses that was prevalent in Ningbo city.


METHODS
Measles viruses were isolated from throat swab specimens of suspected measles cases from 2004 to 2008 and 456 bp fragments of C terminus of nucleprotein (N) gene were amplified by RT-PCR. Sequence analysis was conducted of all 22 virus strains while compared with other measles virus strain which published in GenBank.


RESULTS
All of the 22 measles isolates belonged to genotype H1. The homogeneities of 456 bp fragments of C terminus of nucleprotein (N) gene were as high as 97.1%-98.5%,98.2%-99.6% and 98.5%-98.9% respectively. The nucleotide (amino acids) variability was 8.0%-9.5% (9.8%-14.5%) compared to S191.


CONCLUSIONS
Genotype H1 measles virus circulated in Ningbo, China from 2004 to 2008. H1a was the predominant epidemic strain, H1b strain was existed as well.
Fu
Yan
Y
Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, Zhejiang, China.
Ni
Hong-Xia
HX
Liu
Jian-Yi
JY
D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D005838:Genotype; D006801:Humans; D008457:Measles / Q000453:epidemiology / Q000821:virology*; D008459:Measles virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D014709:Vero Cells
D019590:Nucleocapsid Proteins