title: [Role of leptin in hepatic ischemia/reperfusion-induced hepatic injury].
abstract: OBJECTIVE; To study the changes of leptin after hepatic ischemia/reperfusion (H-I/R) and its effects on H-I/R-induced hepatic injury.
  
  
  METHODS
  A 70% H-I/R model of rats was established. The rats were divided into groups with different reperfusion times and sham-operation group. Radioimmunoassay was applied to measure protein levels of leptin in serum and adipose tissues of the rats. Enzyme-colorimetry was used to detect serum alanine transaminase. Hematoxylin-eosin staining and immunohistochemistry were applied to investigate pathological variations and protein expressions of leptin in livers, respectively. RT-PCR was used to detect leptin mRNA expressions in adipose tissues and livers.
  
  
  RESULTS
  Compared with the sham-operation group, serum leptin increased significantly in the 60 min ischemia/360 min reperfusion (I60' R360') group; protein level of leptin in adipose tissues increased significantly in the I60'R60' group; serum alanine transaminase increased significantly in all of the four reperfusion groups; protein expressions of leptin in livers increased significantly in the I60'R60' and 160'R240' groups; leptin mRNA expression in adipose tissues decreased significantly in the I60'R150' group; leptin mRNA expression in livers increased significantly in the 160'R60' group; leptin mRNA expressions in livers decreased significantly in the I60'R150', I60'R240' and I60'R360' groups. Pathological investigation showed that hepatic impairments at the early phase of H-I/R were more serious. The impairments at the later phase lessened gradually.
  
  
  CONCLUSIONS
  The change of leptin expressions after H-I/R may be a protective factor to withstand H-I/ R-induced hepatic injury.
authors.affiliation: Research Laboratory of Biochemistry, General Hospital of PLA, Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Lin
authors.forename: Guang-Tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Hao
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Lu-Huan
authors.initials: LH
authors.lastname: Wang
mesh_terms: D000818:Animals; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020738:Leptin; D012333:RNA, Messenger