Alcohol biosensing by polyamidoamine (PAMAM)/cysteamine/alcohol oxidase-modified gold electrode.
A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4 degrees C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation.
Akin
Mehriban
M
Faculty of Science, Biochemistry Dept., Ege University, 35100 Bornova-Izmir, Turkiye.
Yuksel
Merve
M
Geyik
Caner
C
Odaci
Dilek
D
Bluma
Arne
A
Höpfner
Tim
T
Beutel
Sascha
S
Scheper
Thomas
T
Timur
Suna
S
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000434:Alcoholic Beverages / Q000032:analysis; D015374:Biosensing Techniques; D049109:Cell Proliferation; D003543:Cysteamine / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013499:Surface Properties
D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D000431:Ethanol; D003543:Cysteamine; D006046:Gold; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase