title: [Single cell gel electrophoresis of a magnesium alloy coated with beta-tricalcium phosphate].
abstract: OBJECTIVE
  To evaluate the genotoxicity of a magnesium alloy coated with beta-tricalcium phosphate (beta-TCP).
  
  
  METHODS
  Four groups were designed. In the first group, AZ31B magnesium alloy surface was coated with beta-TCP using chemical bath deposition, and in the second group magnesium alloy was tested. The other two groups were negative control (pure titanium) and positive control groups (0.5 mg/L bleomycin). Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups, and 60 cells from each group were analysed. Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample. Student-Newman-Keuls (SNK) test was used to compare results from 4 groups.
  
  
  RESULTS
  There were no significant differences in tail moment and tail DNA percentage between magnesium alloy group [(0.52 +/- 0.12), (6.82 +/- 1.81)%] and magnesium alloy coated with beta-TCP group [(0.51 +/- 0.12), (6.89 +/- 1.93)%, P > 0.05]. Tail moment and tail DNA percentage in negative group were (0.47 +/- 0.14) and (6.29 +/- 1.64)%, and tail moment and tail DNA percentage in positive group were (5.17 +/- 1.23) and (22.09 +/- 4.51)%.
  
  
  CONCLUSIONS
  No significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with beta-TCP.
authors.affiliation: Department of Prosthodontics, China Medical University, Shenyang 110002, China.
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Hao
authors.forename: Li-li
authors.initials: LL
authors.lastname: Tan
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Yan
authors.forename: Xiu-lin
authors.initials: XL
authors.lastname: Yan
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Ai
mesh_terms: D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008274:Magnesium; D008422:Materials Testing
chemical_list: D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C485817:beta-tricalcium phosphate; D008274:Magnesium