title: CD34(+) cell selection using small-volume marrow aspirates: a platform for novel cell therapies and regenerative medicine.
abstract: OBJECTIVE
  This study was initiated to determine whether CD34(+) cell selection of small-volume bone marrow (BM) samples could be performed effectively on the Isolex(R) 300i Magnetic Cell Selection System device and whether the results obtained from these samples were comparable with results from large standard-volume samples. The impact on CD34(+) recovery using a full versus half vial of Isolex(R) CD34 reagent and the effects of shipping a post-selection product were evaluated.
  
  
  METHODS
  A protocol to evaluate CD34(+) cell selection with two ranges of smaller volume BM samples (c. 50 mL and c. 100 mL) was developed and instituted at three Production Assistance for Cellular Therapies (PACT) facilities. The study was performed in two phases.
  
  
  RESULTS
  In phase I, the mean post-selection CD34(+) recoveries from the two sizes of samples were 104.1% and 103.3% (smallest and largest volumes, respectively), and mean CD34(+) recoveries were 115.6% and 88.7%, with full and half vials of reagent, respectively. Mean CD34(+) recoveries for post-shipment smaller volume samples were 106.8% and for larger volume samples 116.4%; mean CD34(+) recoveries were 99.9% and 127.4% for post-shipment samples processed with full and half vials of reagent, respectively. In phase II, mean CD34(+) recovery was 76.8% for post-selection samples and 74.0% for post-shipment samples.
  
  
  CONCLUSIONS
  The results suggest that smaller volume BM sample processing on the Isolex(R) system is as efficient or more efficient compared with standard-volume sample processing. Post-processing mean CD34(+) recovery results obtained using a full or half vial of CD34 reagent were not significantly different.
authors.affiliation: University of Minnesota, Minneapolis, Minnesota 55108, USA. mcken020@umn.edu
authors.forename: David H
authors.initials: DH
authors.lastname: McKenna
authors.forename: Sheryl
authors.initials: S
authors.lastname: Adams
authors.forename: Darin
authors.initials: D
authors.lastname: Sumstad
authors.forename: Therese
authors.initials: T
authors.lastname: Sumstad
authors.forename: Diane
authors.initials: D
authors.lastname: Kadidlo
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Gee
authors.forename: April
authors.initials: A
authors.lastname: Durett
authors.forename: Debe
authors.initials: D
authors.lastname: Griffin
authors.forename: Albert
authors.initials: A
authors.lastname: Donnenberg
authors.forename: David
authors.initials: D
authors.lastname: Amrani
authors.forename: Deborah
authors.initials: D
authors.lastname: Livingston
authors.forename: Robert
authors.initials: R
authors.lastname: Lindblad
authors.forename: Deborah
authors.initials: D
authors.lastname: Wood
authors.forename: David
authors.initials: D
authors.lastname: Styers
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D006801:Humans; D011786:Quality Control; D044968:Regenerative Medicine / Q000379:methods*; D018401:Sample Size
chemical_list: D018952:Antigens, CD34