Immunomodulatory effects of zerumbone isolated from roots of Zingiber zerumbet.
In this study, the immunomodulatory effects of zerumbone isolated from Zingiber zerumbet were investigated by evaluating the effects of this compound towards the lymphocytes proliferation (mice thymocytes, mice splenocytes and human human peripheral blood mononuclear cells, PBMC), cell cycle progression and cytokine (interleukin 2 and 12) induction. Lymphocyte proliferation assay showed that zerumbone was able to activate mice thymocytes, splenocytes and PBMC at dosage dependent pattern where the best concentration was 7.5 microg/ml. Flow cytometry analysis showed the highest population of PBMC entered into G2/M phase after treatment for 72 h with 7.5 microg/ml zerumbone. The production of human interleukin-2 and human interleukin-12 cytokines in culture supernatant from zerumbone activated lymphocytes was prominently upregulated at 24 hour and decreased from 48 h to 72 h. The above results indicate that zerumbone can be used as immunomodulatory agent which can react toward the immune cell cytokine production in dosage dependent pattern.
Keong
Yeap Swee
YS
Dept. of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Alitheen
Noorjahan Banu
NB
Mustafa
Shuhaimi
S
Abdul Aziz
Suraini
S
Abdul Rahman
Mashitoh
M
Ali
Abdul Manaf
AM
D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018517:Plant Roots; D012717:Sesquiterpenes / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D019862:Zingiberales
D007155:Immunologic Factors; D007376:Interleukin-2; D012717:Sesquiterpenes; D018664:Interleukin-12; C403304:zerumbone