Monitoring Leptospira strain collections: the need for quality control.
The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections.
Cerqueira
Gustavo M
GM
Biotechnology Centre, Federal University of Pelotas, Pelotas, RS, Brazil. cerqueira@butantan.gov.br
McBride
Alan J A
AJ
Queiroz
Adriano
A
Pinto
Luciano S
LS
Silva
Everton F
EF
Hartskeerl
Rudy A
RA
Reis
Mitermayer G
MG
Ko
Albert I
AI
Dellagostin
Odir A
OA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S