[Surgical model of chronic renal failure: study in rabbits].
OBJECTIVE
To establish a model of chronic renal failure in rabbits, with perspectives of its use for therapeutic and repairing actions.


METHODS
Nineteen males, adults rabbits (New Zealand) randomly distributed into three groups were used: Group 1 - Control (n =5); Group 2-Sham (n =7); and Group 3 - Experimental (n =7). They were anaesthetized by using intramuscular Cetamine, Diazepam and Fentanyl followed by Sevorane with vaporizer device. In Group 3, a bipolar left nephrectomy was carried out and after four weeks, it was also done a right nephrectomy. All the samples of the renal tissue were weighed. The Group 2 was only submitted to both abdominal laparotomies, without nephrectomy. Biochemical evaluations, with urea, creatinina, sodium and potassium measurement; abdominal ultrasound scan; scintigraphy and histological analysis were performed in all animals.


RESULTS
In group 3 there was a progressive increase of urea (p=0.0001), creatinine (p=0.0001), sodium (p = 0,0002) and potassium (p=0,0003). The comparison of these results with those one of the Groups 1 and 2, in all intervals, revealed blood rising with statistical significant level (p < 0,05). In Group 3, the ultrasound scan identified an increasing of the left kidney size, after 16 weeks and at the 4th week the scintigraphy confirmed the loss of 75% of the left renal mass. In the same group, the histological evaluation showed subcapsular and intersticial fibrosis and also tubular regeneration.


CONCLUSIONS
The experimental model of IRC is feasible, with animal's survival in middle term which allows the use of this interval like a therapeutic window for testing different approaches in order to repair the kidney damages.
Costa
Andrei Ferreira Nicolau da
AF
Departamento de Cirurgia, Programa de Pós-Graduação, FM, UFRJ, RJ, BR. vetcosta@gmail.com
Pereira
Lara de Paula Miranda
Lde P
Ferreira
Manoel Luiz
ML
Silva
Paulo Cesar
PC
Chagar
Vera Lucia Antunes
VL
Schanaider
Alberto
A
D000818:Animals; D004195:Disease Models, Animal; D007676:Kidney Failure, Chronic / Q000601:surgery*; D008297:Male; D011817:Rabbits