Complete genome sequence of avian paramyxovirus-3 strain Wisconsin: evidence for the existence of subgroups within the serotype.
The complete consensus genome sequence was determined for avian paramyxovirus (APMV) serotype 3 strain Wisconsin. The genome is 16,182 nucleotides (nt) in length, consisting of six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5', with a 55-nt leader at its 3' end and a 681-nt trailer at its 5' end. Comparison of the APMV-3 strain Wisconsin nt and the aggregate predicted amino acid (aa) sequences with those of APMV-3 strain Netherlands revealed 67% and 78%, identity, respectively. The nt and aa sequence identities between the two APMV-3 strains were lower than between the two antigenic subgroups of human respiratory syncytial virus (81% and 88% identity, respectively) and the two subgroups of human metapeumovirus (80% and 90% identity, respectively). Reciprocal cross-hemagglutination inhibition and cross-neutralization assays using post-infection sera from chickens indicated that strains Wisconsin and Netherlands are highly related antigenically, with only a 2- to 4-fold difference in antibody reactivity between the homologous and heterologous strains. Taken together, our results indicate that the two APMV-3 strains represent a single serotype with two subgroups that differ substantially based on nt and aa sequences, but with only a modest antigenic difference.
Kumar
Sachin
S
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.
Nayak
Baibaswata
B
Samuel
Arthur S
AS
Xiao
Sa
S
Collins
Peter L
PL
Samal
Siba K
SK
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D018109:Avulavirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002642:Chick Embryo; D016000:Cluster Analysis; D003429:Cross Reactions; D005814:Genes, Viral; D016679:Genome, Viral; D005838:Genotype; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D012367:RNA, Viral