Sauchinone reduces oxygen-glucose deprivation-evoked neuronal cell death via suppression of intracellular radical production.
Sauchinone, a biologically active lignan isolated from Saururus chinensis, has been reported to show cytoprotective, anti-inflammatory and anti-apoptotic effects. However, little study has been done of the anti-ischemic/hypoxic effect of sauchinone. The present study investigates the anti-ischemic/hypoxic effect of sauchinone by using ischemia/hypoxia-sensitive neuronal cells. We found that sauchinone significantly prevented cortical neurons from oxygen-glucose deprivation (OGD) followed by re-oxygenation. Sauchinone did not inhibit both NMDA-induced cell membrane depolarization and intracellular calcium influx. We further found that sauchinone cannot directly scavenge reactive oxygen and nitrogen species such as H2O2 and peroxynitrite. Sauchinone, however, greatly reduced the formation of reactive oxygen and nitrogen species in neurons exposed to OGD/reoxygenation and inhibited the depolarization of mitochondrial transmembrane potential induced by OGD/reoxygenation. In accordance with diminishment of endogenous ROS production, sauchinone restored the decreased activities of antioxidant enzymes catalase and SOD evoked by OGD/reoxygenation. Specifically, sauchinone up-regulated the activity of catalase, indicating that sauchinone could be a useful cytoprotectant.
Choi
In Young
IY
Department of Neuroscience and Division of Brain Korea 21 Project for Biomedical Science, College of Medicine, Korea University, Seoul 136-705, Korea.
Yan
Hua
H
Park
Yong-Ki
YK
Kim
Won-Ki
WK
D000818:Animals; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004149:Dioxoles / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000503:physiopathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D032134:Saururaceae / Q000737:chemistry*
D001578:Benzopyrans; D004149:Dioxoles; D018696:Neuroprotective Agents; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C410958:sauchinone; D002374:Catalase; D005947:Glucose; D010100:Oxygen