title: mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are altered in brains from subjects with autism.
abstract: We have shown altered expression of gamma-aminobutyric acid A (GABA(A)) and gamma-aminobutyric acid B (GABA(B)) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA(A) subunits previously associated with autism (GABRalpha4; GABRalpha5; GABRbeta1). Three GABA receptor subunits demonstrated mRNA and protein level concordance in superior frontal cortex (GABRalpha4, GABRalpha5, GABRbeta1) and one demonstrated concordance in cerebellum (GABBetaR1). These results provide further evidence of impairment of GABAergic signaling in autism.
authors.affiliation: Department of Psychiatry, Division of Neuroscience Research, University of Minnesota School of Medicine, 420 Delaware St SE MMC 392, Minneapolis, MN 55455, USA. fatem002@umn.edu
authors.forename: S Hossein
authors.initials: SH
authors.lastname: Fatemi
authors.forename: Teri J
authors.initials: TJ
authors.lastname: Reutiman
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Folsom
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Rooney
authors.forename: Diven H
authors.initials: DH
authors.lastname: Patel
authors.forename: Paul D
authors.initials: PD
authors.lastname: Thuras
mesh_terms: D000328:Adult; D001321:Autistic Disorder / Q000378:metabolism*; D015153:Blotting, Western; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D011963:Receptors, GABA-A / Q000032:analysis*; D018080:Receptors, GABA-B / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C546260:GABRA4 protein, human; C527986:GABRA5 protein, human; C546264:GABRB1 protein, human; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B