title: An aseptic meningitis outbreak caused by echovirus 6 in Anhui province, China.
abstract: An outbreak of aseptic meningitis (AM) occurred in Jinzhai County in Anhui province from April to July in 2005. Totally, 97 children aged 3-15 years were hospitalized. To identify the etiologic agent, 77 cerebrospinal fluid specimens (CSF) and 5 fecal specimens were collected from the patients and cultured by human rhabdomyosarcoma (RD) cell line. Thirty isolates of human echovirus 6 (E6) from 27 CSF and 3 fecal specimens were confirmed by neutralization assay and sequencing analysis of the VP1 gene. The homology of VP1 gene among Anhui isolates was 99.7-100.0% and it indicated that this AM outbreak probable caused by a single transmission link of E6. Phylogenetic analysis based on all the available complete VP1 sequences indicated that E6 could be divided into clusters A, B, and C with at least 15% diversity between clusters and the C cluster could be further divided into C1, C2, C3, and C4. The Anhui isolates most resembled a 2005 strain from Russia (25465 Tambov) and belong to C4. This is the first report that E6 was responsible for an outbreak of AM in China. J. Med. Virol. 82:441-445, 2010. (c) 2010 Wiley-Liss, Inc.
authors.affiliation: WHO WPRO Regional Reference Measles Lab and State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing, People's Republic of China.
authors.forename: Naiying
authors.initials: N
authors.lastname: Mao
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Shujie
authors.initials: S
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Aili
authors.initials: A
authors.lastname: Cui
authors.forename: Yixin
authors.initials: Y
authors.lastname: Ji
authors.forename: Songtao
authors.initials: S
authors.lastname: Xu
authors.forename: Wenbo
authors.initials: W
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D036022:Capsid Proteins / Q000235:genetics; D018929:Cell Culture Techniques; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004196:Disease Outbreaks; D004458:Echovirus 6, Human / Q000302:isolation & purification*; D004457:Echovirus Infections / Q000453:epidemiology*; D005243:Feces / Q000821:virology; D006760:Hospitalization; D006801:Humans; D008582:Meningitis, Aseptic / Q000453:epidemiology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins