Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent.
A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22(T), was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22(T) were slightly curved to straight rods (0.3-0.5×0.6-1.0μm) and motile by a single polar flagellum. Strain LM22(T) was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll a were absent. Growth occurred at 20-40°C (optimum 30-35°C) and pH6.0-10.0 (optimum pH7.0-7.5). Strain LM22(T) did not require NaCl for growth and tolerated up to 2.0 % NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10 % of the total) were C(18 : 1)ω7c (32.9 %), C(19 : 0) cyclo ω8c (18.7 %), C(16 : 0) (12.1 %) and C(16 : 0) 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that Inquilinus limosus AU0476(T) was the closest relative (90.4 % 16S rRNA gene sequence similarity). The DNA G+C content was 65.6&emsp14;mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22(T) was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain of Dongia mobilis is LM22(T) (=CGMCC 1.7660(T) =JCM 15798(T)).
Liu
Ying
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Jin
Jing-Hua
JH
Environmental Protection Research Institute of Light Industry, Beijing 100089, PR China.
Liu
Ying-Hao
YH
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhou
Yu-Guang
YG
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Liu
Zhi-Pei
ZP
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012394:Rosaniline Dyes; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012394:Rosaniline Dyes; D012722:Sewage; C005095:malachite green; D014451:Ubiquinone; C025204:Ubiquinone Q2