Electrotaxis of Caenorhabditis elegans in a microfluidic environment.
The nematode (worm) Caenorhabditis elegans is one of the most widely studied organisms for biomedical research. Currently, C. elegans assays are performed either on petri dishes, 96-well plates or using pneumatically controlled microfluidic devices. In this work, we demonstrate that the electric field can be used as a powerful stimulus to control movement of worms in a microfluidic environment. We found that this response (termed electrotaxis) is directional, fully penetrant and highly sensitive. The characterization of electrotaxis revealed that it is mediated by neuronal activity that varies with the age and size of animals. Although the speed of swimming is unaffected by changes in the electric field strength and direction, our results show that each developmental stage responds to a specific range of electric field with a specific speed. Finally, we provide evidence that the exposure to the electric field has no discernible effect on the ability of animals to survive and reproduce. Our method has potential in precisely controlling, directing, and transporting worms in an efficient and automated manner. This opens up significant possibilities for high-throughput screening of C. elegans for drug discovery and other applications.
Rezai
Pouya
P
Department of Mechanical Engineering, McMaster University, JHE-212/B, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1.
Siddiqui
Asad
A
Selvaganapathy
Ponnambalam Ravi
PR
Gupta
Bhagwati P
BP
D000818:Animals; D002107:Caenorhabditis / Q000502:physiology* / Q000528:radiation effects*; D004574:Electromagnetic Fields; D004780:Environment, Controlled; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008124:Locomotion / Q000502:physiology* / Q000528:radiation effects*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*