Identification of a novel splicing isoform of murine CGI-58.
The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the alpha/beta hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.
Yang
Xingyuan
X
Department of Pediatrics, University of Kentucky, Lexington, KY 40536-0509, USA.
Lu
Xin
X
Liu
Jun
J
D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D041721:3T3-L1 Cells; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008049:Lipase; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology; D051379:Mice; D018613:Microscopy, Confocal; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000378:metabolism; D000217:Acyltransferases
D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D002265:Carboxylic Ester Hydrolases; D008049:Lipase; D020033:Protein Isoforms; D014280:Triglycerides; C492986:Abhd5 protein, mouse; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases