Systematic domain deletion analysis of the major sporulation kinase in Bacillus subtilis.
To characterize the role of the three PAS domains in KinA, the major sporulation kinase in Bacillus subtilis, we constructed a series of systematic PAS domain deletion mutants and analyzed their activities using an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible artificial sporulation induction system, which we have developed recently. The results showed that any one of the three PAS domains is sufficient to maintain the kinase activity and trigger sporulation, if not fully then at least partially, when the protein levels increase beyond a certain level.
Eswaramoorthy
Prahathees
P
Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA.
Fujita
Masaya
M
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D009154:Mutation; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; C075521:kinA protein, Bacillus subtilis; D010770:Phosphotransferases; D011494:Protein Kinases