title: Possible mechanism for the foodweb transfer of covalently bound microcystins.
abstract: Microcystins (MCs) are cyanobacterial toxins that inhibit protein phosphatases 1 and 2A (PP1, PP2A) within an animal through both reversible and covalent interactions. Only MCs that have accumulated in animal tissue in reversible interactions are currently considered when estimating risk to higher trophic levels and humans through food web exposure. However, the majority of MCs is likely covalently bound to target proteins in tissues and these MCs are not quantified or included in these assessments. These covalently bound MCs may be made bioavailable in the digestive system of a consumer through the digestion of their attached protein phosphatase. Three common digestive enzymes, pepsin, chymotrypsin, and trypsin, did not digest cyclic MC-LR and MC-LY, but were very active against a control peptide with typical linkages and standard amino acids in "L" conformation, supporting the possibility for MC-peptide formation during gut passage. To test if digestion products could be biologically active in the consumer, four predicted MC-peptides were synthesized and assayed for activity against PP1 by the protein phosphatase inhibition assay (PPIA). All four MC-peptides were active against PP1 and comparably half (58%) as inhibitory as the parent toxin. This in vitro study demonstrated that MCs covalently bound to proteins may represent a reservoir of potential toxicity for consumers.
authors.affiliation: Department of Environmental and Forest Biology, State University of New York, Syracuse, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Juliette L
authors.initials: JL
authors.lastname: Smith
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Schulz
authors.forename: Paul V
authors.initials: PV
authors.lastname: Zimba
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Boyer
mesh_terms: D020387:Food Chain; D008387:Marine Toxins / Q000737:chemistry* / Q000633:toxicity; D052998:Microcystins / Q000737:chemistry* / Q000633:toxicity; D008956:Models, Chemical; D010447:Peptide Hydrolases / Q000737:chemistry; D018570:Risk Assessment
chemical_list: D008387:Marine Toxins; D052998:Microcystins; D010447:Peptide Hydrolases