Effects of hydroxyapatite and Biostite on osteogenic induction of hMSC.
When isolated from the iliac crest human mesenchymal stem cells (hMSC) differentiate into osteoblast-like cells with appropriate stimulation in culture. This in vitro study tested the hypothesis that Biostite and hydroxyapatite (HA) affect proliferation and differentiation of hMSC into osteoblastic cells. Cell proliferation was determined by measuring 3H-thymidine incorporation into DNA and typical markers of osteoblastic phenotype were determined by RT-PCR assay. No differences emerged in cell proliferation cultures with Biostite or hydroxyapatite (HA), but gene expression analysis revealed higher expression of collagen,alkaline phosphatase (ALP), osteopontin and bone sialoprotein (BSP) in the presence of Biostite. TGFb2 production, as assessed by an Elisa kit, and Runx2 expression by RT-PCR, were greater in Biostite cultures, suggesting Biostite provides a better environment for hMSC differentiation into osteoblasts and is, potentially, a more promising bone-filling material than HA.
Marinucci
Lorella
L
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy. marin.l@unipg.it
Balloni
Stefania
S
Becchetti
Ennio
E
Bistoni
Giovanni
G
Calvi
Edoardo Maria
EM
Lumare
Eleonora
E
Ederli
Filippo
F
Locci
Paola
P
D018786:Bone Substitutes / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage*; D017886:Durapatite / Q000008:administration & dosage*; D006025:Glycosaminoglycans / Q000008:administration & dosage*; D006801:Humans; D006882:Hydroxyapatites / Q000008:administration & dosage*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*
C080295:Biostite; D018786:Bone Substitutes; D006025:Glycosaminoglycans; D006882:Hydroxyapatites; D003094:Collagen; D017886:Durapatite