title: The importance of protein-protein interactions on the pH-induced conformational changes of bovine serum albumin: a small-angle X-ray scattering study.
abstract: The combined effects of concentration and pH on the conformational states of bovine serum albumin (BSA) are investigated by small-angle x-ray scattering. Serum albumins, at physiological conditions, are found at concentrations of approximately 35-45 mg/mL (42 mg/mL in the case of humans). In this work, BSA at three different concentrations (10, 25, and 50 mg/mL) and pH values (2.0-9.0) have been studied. Data were analyzed by means of the Global Fitting procedure, with the protein form factor calculated from human serum albumin (HSA) crystallographic structure and the interference function described, considering repulsive and attractive interaction potentials within a random phase approximation. Small-angle x-ray scattering data show that BSA maintains its native state from pH 4.0 up to 9.0 at all investigated concentrations. A pH-dependence of the absolute net protein charge is shown and the charge number per BSA is quantified to 10(2), 8(1), 13(2), 20(2), and 26(2) for pH values 4.0, 5.4, 7.0, 8.0, and 9.0, respectively. The attractive potential diminishes as BSA concentration increases. The coexistence of monomers and dimers is observed at 50 mg/mL and pH 5.4, near the BSA isoelectric point. Samples at pH 2.0 show a different behavior, because BSA overall shape changes as a function of concentration. At 10 mg/mL, BSA is partially unfolded and a strong repulsive protein-protein interaction occurs due to the high amount of exposed charge. At 25 and 50 mg/mL, BSA undergoes some re-folding, which likely results in a molten-globule state. This work concludes by confirming that the protein concentration plays an important role on the pH-unfolded BSA state, due to a delicate compromise between interaction forces and crowding effects.
authors.affiliation: Instituto de Física da Universidade de São Paulo, São Paulo, Brazil. lbarbosa@if.usp.br
authors.forename: Leandro R S
authors.initials: LR
authors.lastname: Barbosa
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Ortore
authors.forename: Francesco
authors.initials: F
authors.lastname: Spinozzi
authors.forename: Paolo
authors.initials: P
authors.lastname: Mariani
authors.forename: Sigrid
authors.initials: S
authors.lastname: Bernstorff
authors.forename: Rosangela
authors.initials: R
authors.lastname: Itri
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods*; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure*; D014961:X-Ray Diffraction
chemical_list: D012710:Serum Albumin, Bovine