title: In vitro evaluation of cell proliferation and collagen synthesis on titanium following plasma electrolytic oxidation.
abstract: Titania-based coatings produced by plasma electrolytic oxidation are being investigated as bioactive surfaces for titanium implants. In this study, plasma electrolytic oxidation was performed in calcium- and phosphorus-based electrolytes under DC conditions, resulting in coatings of thickness of approximately 8-15 mum. Coating morphologies, microstructures, and compositions were examined by scanning electron microscopy with energy-dispersive X-ray analysis, X-ray diffraction, and electron probe microanalysis. The coatings revealed a cratered morphology, with incorporated calcium and phosphorus species. Proliferation rates of primary human osteoblasts cells on the coatings were up to approximately 37% faster than those for uncoated titanium and 316L stainless steel reference materials. Further, the coatings assisted cell adhesion and generation and anchorage of collagen. The amount of collagen was upto approximately 2.4 times greater than for the reference substrates. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
authors.affiliation: Corrosion and Protection Centre, School of Materials, The University of Manchester, Manchester M60 1QD, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Whiteside
authors.forename: Endzhe
authors.initials: E
authors.lastname: Matykina
authors.forename: Julie E
authors.initials: JE
authors.lastname: Gough
authors.forename: Peter
authors.initials: P
authors.lastname: Skeldon
authors.forename: George E
authors.initials: GE
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000096:biosynthesis*; D004572:Electrolysis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D010758:Phosphorus; D003094:Collagen; D014025:Titanium; D002118:Calcium