[Tracking T lymphocytes proliferation with vital dye CFSE in experimental autoimmune uveitis].
OBJECTIVE
To track T lymphocytes proliferation with vital dye CFSE in experimental autoimmune uveitis (EAU).


METHODS
B10RIII mice were immunized with synthetic peptide of interphotoreceptor retinal-binding protein (IRBP161-180) to develop EAU. The proliferation of IRBP-specific T cell subsets was detected by CFSE staining, fluorescent antibody labeling, and flow cytometry.


RESULTS
The IRBP-specific T cells divided after 4 days of stimulation with IRBP161-180, halving the fluorescence intensity. The proliferations of CD4+ and CD8+ T cells were asynchronous, with CD8+ T cells dividing more vigorously and having more drop in percentage of parent cells (P < 0.01).


CONCLUSIONS
CFSE-Labeling can detect the proliferation of autoreactive T cells and their subsets in EAU effectively.
Lei
Song
S
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Mao
Yong-Qiu
YQ
D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000276:immunology*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005136:Eye Proteins; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012177:Retinol-Binding Proteins; D013388:Succinimides; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D014605:Uveitis / Q000139:chemically induced / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012177:Retinol-Binding Proteins; D013388:Succinimides; D000099214:Retinol-Binding Proteins, Interstitial; C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester