title: Validation of the CDC biofilm reactor as a dynamic model for assessment of encrustation formation on urological device materials.
abstract: Contemporary medical science is reliant upon the rational selection and utilization of devices, and therefore, an increasing need has developed for in vitro systems aimed at replicating the conditions to which urological devices will be subjected to during their use in vivo. We report the development and validation of a novel continuous flow encrustation model based on the commercially available CDC biofilm reactor. Proteus mirabilis-induced encrustation formation on test biomaterial sections under varying experimental parameters was analyzed by X-ray diffraction, infrared- and Raman spectroscopy and by scanning electron microscopy. The model system produced encrusted deposits similar to those observed in archived clinical samples. Results obtained for the system are highly reproducible with encrustation being rapidly deposited on test biomaterial sections. This model will have utility in the rapid screening of encrustation behavior of biomaterials for use in urological applications.
authors.affiliation: School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK. b.gilmore@qub.ac.uk
authors.forename: Brendan F
authors.initials: BF
authors.lastname: Gilmore
authors.forename: Turlough M
authors.initials: TM
authors.lastname: Hamill
authors.forename: David S
authors.initials: DS
authors.lastname: Jones
authors.forename: Sean P
authors.initials: SP
authors.lastname: Gorman
mesh_terms: D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D018441:Biofilms; D019149:Bioreactors; D004866:Equipment Contamination; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D011513:Proteus mirabilis / Q000254:growth & development*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D014546:Urinary Catheterization; D014572:Urology / Q000295:instrumentation*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials