Comprehensive modeling and functional analysis of Toll-like receptor ligand-recognition domains.
Toll-like receptors (TLRs) are innate immune pattern-recognition receptors endowed with the capacity to detect microbial pathogens based on pathogen-associated molecular patterns. The understanding of the molecular principles of ligand recognition by TLRs has been greatly accelerated by recent structural information, in particular the crystal structures of leucine-rich repeat-containing ectodomains of TLR2, 3, and 4 in complex with their cognate ligands. Unfortunately, for other family members such as TLR7, 8, and 9, no experimental structural information is currently available. Methods such as X-ray crystallography or nuclear magnetic resonance are not applicable to all proteins. Homology modeling in combination with molecular dynamics may provide a straightforward yet powerful alternative to obtain structural information in the absence of experimental (structural) data, provided that the generated three-dimensional models adequately approximate what is found in nature. Here, we report the development of modeling procedures tailored to the structural analysis of the extracellular domains of TLRs. We comprehensively compared secondary structure, torsion angles, accessibility for glycosylation, surface charge, and solvent accessibility between published crystal structures and independently built TLR2, 3, and 4 homology models. Finding that models and crystal structures were in good agreement, we extended our modeling approach to the remaining members of the TLR family from human and mouse, including TLR7, 8, and 9.
Kubarenko
Andriy V
AV
Toll-Like Receptors and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany. a.kubarenko@dkfz.de
Ranjan
Satish
S
Colak
Elif
E
George
Julie
J
Frank
Martin
M
Weber
Alexander N R
AN
D000818:Animals; D018360:Crystallography, X-Ray; D006801:Humans; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D016296:Mutagenesis; D017434:Protein Structure, Tertiary; D051193:Toll-Like Receptors / Q000737:chemistry* / Q000235:genetics
D008024:Ligands; D051193:Toll-Like Receptors