Concentrations of cadmium and zinc in seawater of Bohai Bay and their effects on biomarker responses in the bivalve Chlamys farreri.
Both in-field chemical investigation and in the laboratory toxic tests were carried out to systematically understand the pollution status of cadmium (Cd) and zinc (Zn) in Bohai Bay. Samples collected from surface seawater were determined to describe the distributions of Cd and Zn in Bohai Bay. The average values in our study of Cd and Zn were 0.15 microg/L and 19.68 microg/L, respectively. Both of them were lower than the first class limit of seawater quality standard in China. In the laboratory, antioxidant enzymes [SOD (Cu/Zn-SOD, Mn-SOD), CAT], lipid peroxidation (MDA), phase I and phase II enzymes (CYP4501A and GST) were investigated in the bivalves Chlamys farreri exposed to Cd and Zn at the concentration levels of Bohai Bay seawater, which were obtained from our in-field investigation. The reduced SOD, CAT, and EROD (7-ethoxyresorufin-O-deethylase) activities (with the inhibitory rate of 16.8%, 31.5%, and 51.6%, respectively) in Cd treatment were observed and resulted in obvious lipid peroxidation damage. However, treatment of Zn showed elevations in SOD and GST by 13.3% and 29.9%, respectively, and with no influence on lipid peroxidation. In summary, seawater quality in Bohai Bay seawater was ranked as good in general, but it seemed that Cd might possess a potential environmental risk by effecting pro-oxidant/antioxidant balance and phase I detoxification in C. farreri.
Zhang
Ying
Y
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, People's Republic of China. zhangying405@ms.qdio.ac.cn
Song
Jinming
J
Yuan
Huamao
H
Xu
Yayan
Y
He
Zhipeng
Z
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000032:analysis* / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D049897:Pectinidae / Q000187:drug effects* / Q000378:metabolism; D012623:Seawater / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000032:analysis* / Q000633:toxicity
D015415:Biomarkers; D002104:Cadmium; D002374:Catalase; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D015032:Zinc