title: Multiplex PCR assay to facilitate identification of the recently described Staphylococcal cassette chromosome mec type VIII.
abstract: We recently described a novel staphylococcal cassette chromosome mec (SCCmec) element, designated type VIII. We report here a multiplex PCR assay, capable of identifying SCCmec type VIII. This assay will facilitate identification of this new SCCmec type and provides a useful tool for clinical and epidemiologic studies in this regard.
authors.affiliation: Centre for Antimicrobial Resistance, Alberta Health Services/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada.
authors.forename: Jo-Ann
authors.initials: JA
authors.lastname: McClure
authors.forename: John M
authors.initials: JM
authors.lastname: Conly
authors.forename: Sameer
authors.initials: S
authors.lastname: Elsayed
authors.forename: Kunyan
authors.initials: K
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001681:Biological Assay; D002876:Chromosomes, Bacterial / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*