title: [Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress].
abstract: OBJECTIVE
  To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.
  
  
  METHODS
  BALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.
  
  
  RESULTS
  In control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).
  
  
  CONCLUSIONS
  The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.
authors.affiliation: Deparment of Prosthodontics, Sun Yat-sen University, Guangzhou 510055, China.
authors.forename: Ya-juan
authors.initials: YJ
authors.lastname: Xu
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Chang-jing
authors.initials: CJ
authors.lastname: Wu
authors.forename: You-rui
authors.initials: YR
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012212:Rheology; D013314:Stress, Mechanical
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015638:Cytochalasin D