Effects of scoparone on dopamine release in PC12 cells.
The effects of scoparone on dopamine release in PC12 cells were investigated. Scoparone at 50-200 microM increased dopamine release into the culture medium. However, the released levels of dopamine by scoparone were not altered in the absence of extracellular Ca(2+) and by adenylyl cyclase inhibitor MDL-12,330A. Scoparone increased phosphorylation of PKA, CaMK II and synapsin I. Scoparone also enhanced K(+)-induced levels of dopamine release by CaMK II phosphorylation. These results suggest that scoparone increases dopamine release by synapsin I phosphorylation via activation of PKA and CaMK II, which are mediated by cyclic AMP levels and Ca(2+) influx.
Yang
Yoo Jung
YJ
College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Lee
Hak Ju
HJ
Lee
Byung Koo
BK
Lim
Sung Cil
SC
Lee
Chong Kil
CK
Lee
Myung Koo
MK
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D007097:Imines / Q000494:pharmacology; D016716:PC12 Cells; D010766:Phosphorylation / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D016704:Synapsins / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology*
D000067956:Adenylyl Cyclase Inhibitors; D003374:Coumarins; D007097:Imines; D016704:Synapsins; D014665:Vasodilator Agents; C014925:RMI 12330A; D017868:Cyclic AMP-Dependent Protein Kinases; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; C018145:scoparone; D011188:Potassium; D004298:Dopamine