title: Detection of Cry1Ab toxin in the leaves of MON 810 transgenic maize.
abstract: The distribution of Cry1Ab toxin was detected in the leaves of genetically modified maize of genetic event MON 810 by enzyme-linked immunosorbent assay. Cry1Ab toxin contents in the leaves at reproductive (milk, R3) phenological stage were measured to be between 3,878 and 11,148 ng Cry1Ab toxin/g fresh weight. Toxin content was significantly lesser (significant difference (SD) = 1,823 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) in leaves at the lowest leaf level, than at higher leaf levels, probably due to partial leaf necrotisation. A substantial (up to 22%) plant-to-plant variation in Cry1Ab contents in leaves was observed. When studying toxin distribution within the cross and longitudinal sections of single leaves, lesser variability was detected diagonally, with approximately 20% higher toxin concentrations at or near the leaf vein. More significant variability (SD = 2,220 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) was seen lengthwise along the leaf, starting at 1,892 ng Cry1Ab toxin/g fresh weight at the sheath and rising to maximum concentration at the middle of the lamella. Cry1Ab toxin content may suffer significant (SD = 2,230 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) decreases in the leaf due to necrotisation. The results indicate that the longitudinal dimension of the leaf has more significance for sampling purposes than the diagonal position.
authors.affiliation: Department of Ecotoxicology and Environmental Analysis, Plant Protection Institute, Hungarian Academy of Sciences, Herman Ottó u. 15, 1022 Budapest, Hungary. aszek@nki.hu
authors.forename: András
authors.initials: A
authors.lastname: Székács
authors.forename: Eva
authors.initials: E
authors.lastname: Lauber
authors.forename: Eszter
authors.initials: E
authors.lastname: Takács
authors.forename: Béla
authors.initials: B
authors.lastname: Darvas
mesh_terms: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004731:Endotoxins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis