title: CE determination of secaloindoline allelic forms in hexaploid triticale (x Triticosecale Wittmack).
abstract: Differences in kernel texture are mainly caused by specific secaloindoline (SIN) proteins occurring in friabilin fraction of hexaploid triticale (x Triticosecale Wittmack) grain. SINs were isolated using Triton X-114 partitioning from either kernels/flour or starch of five triticale cultivars with wide range of different hardness. Crude SIN fraction was obtained by size-exclusion HPLC. SINs were separated on an uncoated fused-silica capillary using the iminodiacetic (IDA) buffer in conjunction with lower-concentrated poly(ethylene oxide) and ACN. A low-concentrate mixture of hydrophilic polymers, PVP and hydroxypropylmethylcellulose in IDA buffer was employed for dynamic coating of capillary inner wall. In total, on the basis of CZE profiles, two SIN-a proteins and two SIN-b proteins were identified. Allelic forms SIN-a1 and SIN-b1 have both two soft and one medium hard genotypes, however other allelic forms, designed as SIN-a2 and SIN-b2, were identified in hard and other medium hard cultivars. The CZE profiles showed that the ratio of the peak areas of SIN-b proteins isolated from triticale starch can be preliminarily used to distinguish cultivars with soft and hard grain.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland. bsal@igr.poznan.pl
authors.forename: Bolesław P
authors.initials: BP
authors.lastname: Salmanowicz
mesh_terms: D000483:Alleles; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D056625:Endosperm / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics; D012434:Secale / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*
chemical_list: D010940:Plant Proteins; C082942:puroindoline protein, Triticum aestivum; C092997:RKIN1 protein, Secale cereale; D017346:Protein Serine-Threonine Kinases