title: [Genetype characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008].
abstract: Genetic characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008 were analyzed. All samples were detected by RT-PCR using EV71-specific primers. The VP1s of EV71 strains were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with those of subgenotype A, B and C using DNASTAR, BioEdit and Mega 3.1 softwares. The VP1 nucleotide sequences of 17 strains of Shenzhen shared 95.3%-99.4% identities with cluster C4a, and one strain shared 93.0%-95.6% identities with cluster C4b. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was 81.8%-86.0%. Among 18 strains, the homogeneity of the VP1 nucleotide sequence was between 92.5%-100%. All EV71 strains circulating in Shenzhen were of subgenotype C4. The predominant strain of EV71 belonged to cluster C4a, also there was cluster C4b.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. heyaqing1019@126.com
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: He
authors.forename: Rui
authors.initials: R
authors.lastname: Qi
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Xian
authors.forename: Xiang-Jie
authors.initials: XJ
authors.lastname: Yao
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Zhang
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Yang
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Xu
mesh_terms: D002460:Cell Line; D002681:China; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins