SERS aptasensor from nanorod-nanoparticle junction for protein detection.
A multicomponent nanostructure comprising of gold nanorod-nanoparticle (AuNR-AuNP) composites was fabricated to detect thrombin at subnanomolar concentrations in diluted human blood serum. Simulation and experiments revealed that the strong electromagnetic coupling resonance at the nanorod-nanoparticle junction of these probes can be used to construct highly sensitive SERS aptasensors.
Wang
Yuling
Y
Physiological Sensing Facility, Bindley Biosciences Center and Birck Nanotechnology Center, Purdue University 225 S. University Street, 215 ABE Building, West Lafayette, IN 47907, USA.
Lee
Kyuwan
K
Irudayaraj
Joseph
J
D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D011485:Protein Binding; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D012710:Serum Albumin, Bovine; D006046:Gold; D013917:Thrombin