Down-regulation of Stat3 decreases invasion activity and induces apoptosis of human glioma cells.
Gliomas are the most common type of primary brain tumors. Despite the improvement in current treatments for gliomas, including surgical resection, radiation, and chemotherapy, there has been very little progress in curing this kind of disease. Stat3 is a member of signal transducer and activator of transcription family. It plays an important role in regulating cell survival, invasion, and apoptosis. This study investigated the influence of low-level expression of Stat3 on invasion and apoptosis in U251 cells. Our data showed that Stat3 is constitutively expressed in human gliomas cell line U251. The invasion activity in U251 cells was weakened and the apoptosis in U251 cells was induced after down-regulation of Stat3. In addition, down-regulation of Stat3 can suppress the expression of MMP-2, Bcl-xL and survivin but not 67LR. These results further indicate that Stat3 plays a key role in the invasion and apoptosis of human glioma cell line U251.
Chen
Fuxue
F
Laboratory of Molecular Biology, School of Life Sciences, Shanghai University, Shanghai, 200436, People's Republic of China. chenfuxue@staff.shu.edu.cn
Xu
Yaming
Y
Luo
Youqun
Y
Zheng
Da
D
Song
Yichao
Y
Yu
Kangkang
K
Li
Hong
H
Zhang
Li
L
Zhong
Weiwei
W
Ji
Yonghua
Y
D017209:Apoptosis / Q000502:physiology*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D000077022:Survivin; D051020:bcl-X Protein / Q000378:metabolism
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; D000077022:Survivin; D051020:bcl-X Protein; D020778:Matrix Metalloproteinase 2