FRT-seq: amplification-free, strand-specific transcriptome sequencing.
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
Mamanova
Lira
L
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
Andrews
Robert M
RM
James
Keith D
KD
Sheridan
Elizabeth M
EM
Ellis
Peter D
PD
Langford
Cordelia F
CF
Ost
Tobias W B
TW
Collins
John E
JE
Turner
Daniel J
DJ
D002874:Chromosome Mapping / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
D014157:Transcription Factors