title: Effect of amphotericin B on the infection success of Schistosoma mansoni in Biomphalaria glabrata.
abstract: In the present study, we examined the effect of amphotericin B on larval stages (miracidia and primary sporocyst) of the helminth Schistosoma mansoni, the causative agent of human schistosomiasis. Amphotericin B (AmB) is a polyene macrolide that disturbs the function of the cell membrane; it is widely used as prophylactic antimycotic agent in in vitro culture. We show for the first time that S. mansoni miracidia infectivity is considerably reduced after AmB treatment. Moreover we demonstrate that AmB does not affect the development, growth, viability, and behavior of miracidia and primary sporocysts. Our data indicate that AmB effects on S. mansoni sporocyst prevalence are linked to the oxidative properties of AmB. These may alter the capacity of sporocysts to respond to the oxidative stress generated by the snail immune defence system.
authors.affiliation: Parasitologie Fonctionnelle et Evolutive, UMR 5244, CNRS, EPHE, UPVD, Biologie & Ecologie Tropicale et Méditerranéenne, Université de Perpignan, 52 Ave. Paul Alduy, 66860 Perpignan Cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Moné
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mitta
authors.forename: David
authors.initials: D
authors.lastname: Duval
authors.forename: Benjamin E F
authors.initials: BE
authors.lastname: Gourbal
mesh_terms: D000563:Amebicides / Q000494:pharmacology*; D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D001700:Biomphalaria / Q000469:parasitology*; D006224:Cricetinae; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism; D009068:Movement / Q000187:drug effects; D033661:Oocysts / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress; D010400:Penicillin G / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000563:Amebicides; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D000666:Amphotericin B; D010400:Penicillin G; D013307:Streptomycin