title: Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.
abstract: How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Zhenggen
authors.initials: Z
authors.lastname: Huang
authors.forename: Junjie
authors.initials: J
authors.lastname: Yang
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.forename: Chengjun
authors.initials: C
authors.lastname: Gan
authors.forename: Wenguang
authors.initials: W
authors.lastname: Cheng
authors.forename: Shunzong
authors.initials: S
authors.lastname: Yuan
authors.forename: Xu
authors.initials: X
authors.lastname: Peng
authors.forename: Jianglin
authors.initials: J
authors.lastname: Tan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Shiwei
authors.initials: S
authors.lastname: Yang
authors.forename: Yair
authors.initials: Y
authors.lastname: Reisner
authors.forename: Liangpeng
authors.initials: L
authors.lastname: Ge
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D012867:Skin / Q000166:cytology / Q000196:embryology*; D013552:Swine; D013724:Teratoma / Q000473:pathology*