[Simultaneous determination of nine phenolic acids in Salvia miltiorrhiza by RRLC-UV].
OBJECTIVE
To establish a RRLC-UV method for simultaneous determination of nine phenolic acids in Salvia miltiorrhiza.


METHODS
3,4-Dihydroxyphenylacetic acid was used as internal standard, and analysis was carried out on an Agilent Zorbax Eclipse Plus C18 (2.1 mm x 50 mm, 1.8 microm) column with acetonitrile-0.1% formic acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.3 mL x min(-1), and the UV detector was monitored at 286 nm.


RESULTS
All calibration curves showed good linear regression within test ranges (r > or = 0.999 5); and the overall recoveries were in the range of 98.2%-102.6%, with RSD less than 3.1% (n = 3). The overall RSD of precision test were less than 2.4%.


CONCLUSIONS
The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of S. miltiorrhiza.
Wang
Li
L
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhao
Mingbo
M
He
Wenshun
W
Zan
Ke
K
Bi
Kaishun
K
Tu
Pengfei
P
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C017616:phenolic acid