title: The Toll immune signaling pathway control conserved anti-dengue defenses across diverse Ae. aegypti strains and against multiple dengue virus serotypes.
abstract: Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We have previously demonstrated the involvement of the Toll pathway in the anti-dengue defense at 7 days after infection. In the present study, we have investigated the activity of this immune signaling pathway against different dengue virus serotypes at the early stages of infection in laboratory and field-derived mosquito strains. Our studies corroborate the importance of the Toll pathway in the anti-dengue defense repertoire at 3 days after an infectious blood meal, when new virions are released from the midgut for dissemination and infection of other mosquito tissues. These immune defenses are furthermore conserved among different Ae. aegypti strains and can act against a broad range of dengue virus serotypes.
authors.affiliation: W. Harry Feinstone Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205-2179, USA.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Ramirez
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
mesh_terms: D050153:Abdominal Fat / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D000330:Aedes / Q000276:immunology* / Q000821:virology; D000818:Animals; D002460:Cell Line; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation; D014771:Virion / Q000378:metabolism; D014774:Virulence
chemical_list: C501431:REL1 protein, Aedes aegypti; D034741:RNA, Small Interfering; D051193:Toll-Like Receptors; D014157:Transcription Factors