title: Variable DNA methylation of transposable elements: the case study of mouse Early Transposons.
abstract: Phenotypic variation stems from both genetic and epigenetic differences between individuals. In order to elucidate how phenotypes are determined, it is necessary to understand the forces that generate variation in genome sequence as well as its epigenetic state. In both contexts, transposable elements (TEs) may play an important role. It is well established that TE activity is a major generator of genetic variation, but recent research also suggests that TEs contribute to epigenetic variation. Stochastic epigenetic silencing of some TE insertions in mice has been shown to cause phenotypic variability between individuals. However, the prevalence of this phenomenon has never been evaluated. Here, we use 18 insertions of a mouse Endogenous Retrovirus (ERV) family, the Early Transposons (ETns), to detect insertion-dependent determinants of DNA methylation levels and variability between both cells and individuals. We show that the structure and age of insertions influence methylation levels and variability, resulting in a subgroup of loci that displays unexpectedly high variability in methylation and suggesting stochastic events during methylation establishment. Despite variation in methylation according to the age and structure of each locus, homologous CpG sites show similar tendencies in methylation levels across loci, emphasizing the role of the insertion's sequence in methylation determination. Our results show that differences in methylation of ETns between individuals is not a sporadic phenomenon and support the hypothesis that ERVs contribute to phenotypic variability through their stochastic silencing.
authors.affiliation: Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC, Canada.
authors.forename: Daphne
authors.initials: D
authors.lastname: Reiss
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Arefeh
authors.initials: A
authors.lastname: Rouhi
authors.forename: Max
authors.initials: M
authors.lastname: Reuter
authors.forename: Dixie L
authors.initials: DL
authors.lastname: Mager
mesh_terms: D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D004251:DNA Transposable Elements; D044127:Epigenesis, Genetic; D014644:Genetic Variation; D016678:Genome; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D010641:Phenotype; D012313:RNA / Q000235:genetics; D013269:Stochastic Processes; D014018:Tissue Distribution
chemical_list: D004251:DNA Transposable Elements; D012313:RNA