Initial pattern of angiogenesis and bone formation following lateral ridge augmentation using rhPDGF and guided bone regeneration: an immunohistochemical study in dogs.
OBJECTIVE
To evaluate (i) the effects of rhPDGF-BB on localized ridge augmentation using a natural bone mineral (NBM), and (ii) the influence of a collagen membrane (CM) on factor activity.


METHODS
Chronic-type alveolar ridge defects (n = 4 dogs) were randomly allocated in a split-mouth design as follows: upper jaw: NBM + rhPDGF-BB + CM (test) vs. NBM + rhPDGF-BB (control), and lower jaw: NBM + rhPDGF-BB + CM (test) vs. NBM + CM (control). After 3 weeks, dissected blocks were prepared for immunohistochemical (angiogenesis - TG) and histomorphometrical analysis [e.g. augmented area (AA), mineralized - (MT), non-mineralized tissue (NMT) (mm(2))].


RESULTS
Lower jaw: TG and mineralization of AA mainly originated from the defect borders. Test sites revealed a pronounced TG antigen reactivity and higher AA and MT values (mean and median). Upper jaw: control sites revealed a dislocation of AA in caudal direction, but also an improved vascularization in the peripheral wound area. While MT values (median) appeared to be comparable in both groups, AA, NMT, and NBM values (mean and median) tended to be higher at test sites.


CONCLUSIONS
It was concluded that (i) rhPDGF-BB soak-loaded on NBM might have the potential to support bone formation at chronic-type lateral ridge defects, and (ii) the application of CM did not seem to interfere with the factor activity, but ensured a stabilization of the graft particles.
Schwarz
Frank
F
Department of Oral Surgery, Westdeutsche Kieferklinik, Heinrich Heine University, D-40225 Düsseldorf, Germany. Frank.Schwarz@med.uni-duesseldorf.de
Ferrari
Daniel
D
Podolsky
Laura
L
Mihatovic
Ilja
I
Becker
Jürgen
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D000077214:Becaplermin; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D004285:Dogs; D048091:Guided Tissue Regeneration / Q000379:methods*; D007124:Immunoenzyme Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014081:Tooth Extraction
D018786:Bone Substitutes; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase