title: Systemic loxoscelism confirmation by bite-site skin surface: ELISA.
abstract: We report here a case of systemic loxoscelism, confirmed by bite-site skin surface swab. Features of systemic loxoscelism present in this case included debilitating symptoms, a classic local bite-site reaction, hemolysis causing loss of approximately 15% of the blood volume within 72 hours, and a symptomatic exanthem. A skin surface ELISA test was used to confirm the presence of venom. This test enables confirmation of cases of loxoscelism for which no spider is found.
authors.affiliation: Division of Dermatology, University of Missouri School of Medicine, MO, USA. wvs@mst.edu
authors.forename: William V
authors.initials: WV
authors.lastname: Stoecker
authors.forename: Gary S
authors.initials: GS
authors.lastname: Wasserman
authors.forename: David A
authors.initials: DA
authors.lastname: Calcara
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Green
authors.forename: Karen
authors.initials: K
authors.lastname: Larkin
mesh_terms: D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D054796:Drug Dosage Calculations; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D001098:Spider Bites / Q000175:diagnosis* / Q000188:drug therapy / Q000503:physiopathology*; D013111:Spider Venoms / Q000032:analysis*
chemical_list: D000305:Adrenal Cortex Hormones; D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases