Red-white-blue emission switching molecular beacons: ratiometric multicolour DNA hybridization probes.
Molecular beacons with two fluorophores derived from ethynyl pyrene and ethynyl nile red exhibit red emission in the hairpin that changes to blue through white upon binding to the target sequence with a dramatic shift of approximately 225 nm.
Varghese
Reji
R
Univeristy of Regensburg, Institute for Organic Chemistry, Universitätsstr. 31, D-93053 Regensburg, Germany.
Wagenknecht
Hans-Achim
HA
D001483:Base Sequence; D003116:Color; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D015335:Molecular Probes; D009838:Oligodeoxyribonucleotides; D010078:Oxazines; D011721:Pyrenes; D004247:DNA; C030984:pyrene; C044808:nile red