Identification and quantitation of amphetamine, methamphetamine, MDMA, pseudoephedrine, and ephedrine in blood, plasma, and serum using gas chromatography-mass spectrometry (GC/MS).
Amphetamine, methamphetamine, MDMA, pseudoephedrine, and ephedrine are measured in blood, serum, and plasma using gas chromatography coupled to mass spectrometry (GC/MS). Following a simple liquid-liquid extraction, analytes are derivatized with heptafluorobutyric anhydride (HFBA) and 1 microL injected onto a HP-5MS 15-meter capillary column. Quantitation of each analyte is accomplished using a multi-point calibration curve and deuterated internal standards. The method provides a simple, robust, and reliable means to identify and measure these analytes.
Gunn
Josh
J
AIT Laboratories, Indianapolis, IN, USA.
Kriger
Scott
S
Terrell
Andrea R
AR
D000661:Amphetamine / Q000097:blood*; D004809:Ephedrine / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008694:Methamphetamine / Q000097:blood*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000097:blood*; D054199:Pseudoephedrine / Q000097:blood*; D015203:Reproducibility of Results
D008694:Methamphetamine; D054199:Pseudoephedrine; D000661:Amphetamine; D004809:Ephedrine; D018817:N-Methyl-3,4-methylenedioxyamphetamine