Ganodermasides A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma lucidum on yeast via UTH1 gene.
Two novel ergosterol derivatives, ganodermasides A and B, hydroxylated at C-15 were isolated from the methanol extract of spores of a medicinal mushroom, Ganoderma lucidum, showed to extend the replicative life span of Saccharomyces cerevisiae, a yeast of K6001 strain. The stereostructures of ganodermasides A and B were determined based on the spectroscopic analysis and comparison of spectroscopic data. These new sterols have a 4, 6, 8(14), 22-tetraene-3-one unit with a unique hydroxylation at C-15. The anti-aging activity of these compounds on yeast is comparable to a well-known substance, resveratrol. Based on results of the investigation of the mechanism of biological activity, ganodermasides A and B regulated UTH1 expression in order to extend the replicative life span of yeast.
Weng
Yufang
Y
College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 388, Hangzhou 310058, China.
Xiang
Lan
L
Matsuura
Akira
A
Zhang
Yang
Y
Huang
Qianming
Q
Qi
Jianhua
J
D000375:Aging / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004875:Ergosterol / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D015394:Molecular Structure; D020940:Reishi / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C116477:UTH1 protein, S cerevisiae; D004875:Ergosterol