Rapid and reliable detection of 11 food-borne pathogens using thin-film biosensor chips.
Traditional methods for identifying food-borne pathogens are time-consuming and laborious, so it is necessary to develop innovative methods for the rapid identification of food-borne pathogens. Here, we report the development of silicon-based optical thin-film biosensor chips for sensitive detection of 11 food-borne pathogens. Briefly, aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface, and then, biotinylated polymerase chain reaction (PCR) amplicons were hybridized with the probes. After washing and brief incubation with an antibiotin immunoglobulin G-horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated chains bound to the probes were visualized as a color change on the chip surface (gold to blue/purple). Highly sensitive and accurate examination of PCR fragment targets can be completed within 30 min. This assay is extremely robust, sensitive, specific, and economical and can be adapted to different throughputs. Thus, a rapid, sensitive, and reliable technique for detecting 11 food-borne pathogens was successfully developed.
Bai
Sulan
S
Key Laboratory of Genetics and Biotechnology, College of Life Sciences, Capital Normal University, Beijing, People's Republic of China.
Zhao
Jinyi
J
Zhang
Yaochuan
Y
Huang
Wensheng
W
Xu
Shi
S
Chen
Haodong
H
Fan
Liu-Min
LM
Chen
Ying
Y
Deng
Xing Wang
XW
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity