title: Increased expression of prohibitin and its relationship with poor prognosis in esophageal squamous cell carcinoma.
abstract: Prohibitin, a potential tumor suppressor, has been shown to be an anti- proliferative protein, a regulator of cell-cycle progression and in apoptosis. Recently, it was found to be over-expressed in breast cancer and gastric cancer, and it has been suggested as a biomarker in those diseases. To clarify the role and the prognostic significance of prohibitin expression in esophageal squamous cell carcinoma (ESCC), we analyzed the expression in ESCC and their corresponding nonneoplastic epithelia tissues by immunohistochemistry(IHC), Western blotting and real-time quantitative reverse transcription polymerase chain reaction(QRT-PCR).The relationship between prohibitin expression and clinicopathological variables was examined by statistical analysis. The findings suggested the up-regulation of prohibitin play an important role in the carcinogenesis of ESCC. The over-expression of prihibitin was significantly correlated with the depth of tumor, lymph node metastasis, distant metastasis, lymphatic invasion and vascular invasion of ESCC. These results suggested that prohibitin(+), lymph node metastasis and distant metastasis could be the independent risk factors for worse prognosis in ESCC patients.
authors.affiliation: Department of Pathology, Xiangya Basic Medical College, Central South University, Changsha, China.
authors.forename: Hong-Zheng
authors.initials: HZ
authors.lastname: Ren
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Pan
authors.forename: Ji-Fang
authors.initials: JF
authors.lastname: Wen
authors.forename: Hua
authors.initials: H
authors.lastname: Fu
authors.forename: Xu-zheng
authors.initials: XZ
authors.lastname: Shan
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016009:Chi-Square Distribution; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D000091402:Prohibitins; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016019:Survival Analysis; D046888:Tissue Array Analysis
chemical_list: D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins