Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids.
A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75°C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H(2) and CO(2) using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3-94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1(T) (=DSM 19444(T)=JCM 14716(T)).
Steinsbu
Bjørn O
BO
Department of Earth Science, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.|Centre for Geobiology, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
Thorseth
Ingunn H
IH
Department of Earth Science, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.|Centre for Geobiology, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
Nakagawa
Satoshi
S
Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Inagaki
Fumio
F
Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Monobe B200, Nankoku, Kochi 783-8502, Japan.
Lever
Mark A
MA
Department of Marine Sciences, University of North Carolina at Chapel Hill, 12-7 Venable Hall CB #3300, Chapel Hill, North Carolina 27599, USA.
Engelen
Bert
B
Institut für Chemie und Biologie Des Meeres (ICBM), Carl von Ossietzky Universität Oldenburg, Carl von Ossietzky Strasse 9-11, D-26111 Oldenburg, Germany.
Øvreås
Lise
L
Department of Biology, University of Bergen, Jahnebakken 5, N-5007 Bergen, Norway.|Centre for Geobiology, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
Pedersen
Rolf B
RB
Department of Earth Science, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.|Centre for Geobiology, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
D019609:Archaeoglobus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism
D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D013431:Sulfates