Coupling genetics and post-genomic approaches to decipher the cellular splicing code at a systems-wide level.
Nuclear RNA processing is a critical stage in eukaryotic gene expression, and is controlled in part by the expression and concentration of nuclear RNA-binding proteins. Different nuclear RNA-binding proteins are differentially expressed in different cells, helping the spliceosome to decode pre-mRNAs into alternatively spliced mRNAs. Recent post-genomic technology has exposed the complexity of nuclear RNA processing, and is starting to reveal the mechanisms and rules through which networks of RNA-binding proteins can regulate multiple parallel pathways. Identification of multiple parallel processing pathways regulated by nuclear RNA-binding proteins is leading to a systems-wide understanding of the rules and consequences of alternative nuclear RNA processing.
Liu
Yilei
Y
Institute of Human Genetics, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.
Elliott
David J
DJ
D016678:Genome; D023281:Genomics / Q000379:methods; D006801:Humans; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000378:metabolism*; D052156:SELEX Aptamer Technique
D012322:RNA Precursors; D016601:RNA-Binding Proteins