Transgenic labeling of hair cells in the zebrafish acousticolateralis system.
The zebrafish provides a useful experimental system for investigations of aural development. To permit the controlled expression of transgenes in developing hair cells, we isolated the genomic control regions of the parvalbumin 3a (pvalb3a) and parvalbumin 3b (pvalb3b) genes. Deletion analysis and somatic-cell transgenesis restricted the cis-acting control regions for hair cells to as little as 484base pairs for pvalb3a and 650base pairs for pvalb3b. Using both meganuclease-mediated and standard methods, we produced transgenic animals that transmit transgenes through their germ lines. These fish express GFP in hair cells in the inner ear and lateral line. Two stable transgenic lines express GFP prior to hair-bundle formation, so the associated promoter constructs are suitable for manipulating gene expression during bundle development. We additionally identified a transgenic line that offers variable labeling of supporting cells.
McDermott
Brian M
BM
Laboratory of Sensory Neuroscience and Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue New York, NY 10065, USA.
Asai
Yukako
Y
Baucom
Jessica M
JM
Jani
Shraddha D
SD
Castellanos
Yaneth
Y
Gomez
Gustavo
G
McClintock
James M
JM
Starr
Catherine J
CJ
Hudspeth
A J
AJ
D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D006198:Hair Cells, Auditory / Q000378:metabolism*; D010320:Parvalbumins / Q000235:genetics*; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics*
D010320:Parvalbumins; D029961:Zebrafish Proteins; D049452:Green Fluorescent Proteins