title: CASPASE-3 and CD-34 expression in prostate adenocarcinoma.
abstract: OBJECTIVE
  1. To evaluate the percentage of caspase-3 and CD-34 expression on adenocarcinoma; 2. to quantify caspase-3 and CD-34 in tumor cells; 3. to verify the relationship between biomarkers and its malignancy; 4. to correlate biomarkers themselves.
  
  
  METHODS
  Thirty-eight human malignant prostate specimens, Gleason's score, were immunohistochemically stained for caspase-3 and CD-34 protein. Quantification was done under Samba 4000 Immuno System reading, yielding two variables: label index and optical density. Statistical analyses were based on cross-methods involving univariate and bivariate as well as correlation factors among independent variables.
  
  
  RESULTS
  Immunostaining was revealed in 25 plates for caspase-3 and 34 for CD-34. Caspase-3 expression for label index was over 50 in 76%, while for optical density was below 50 in 96%. CD-34 expression demonstrated label index over 50 in 59% and optical density below 50 in 56%. Correlation among expression and severity did not demonstrate to be statistically significant. There was no correlation between protein expression and Gleason's score.
  
  
  CONCLUSIONS
  1. Caspase-3 and CD-34 were present respectively in 73.5% and 100% of samples; 2. caspase-3 and CD-34 showed high expression regarding label index and low expression in optical density; 3. there was no statistical significance among expressions and tumor severity according to Gleason's score; 4. no significant correlation could be set between the biomarkers themselves.
authors.affiliation: Principles of Surgery Post-Graduate Program, Evangelical Medical School, Curitiba, Paraná, Brazil.
authors.forename: Vicente Paulo
authors.initials: VP
authors.lastname: da Motta
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.forename: Jurandir Marcondes
authors.initials: JM
authors.lastname: Ribas-Filho
authors.forename: Nicolau Gregori
authors.initials: NG
authors.lastname: Czeczko
authors.forename: Carmen Austrália Paredes Marcondes
authors.initials: CA
authors.lastname: Ribas
authors.forename: Ronaldo Máfia
authors.initials: RM
authors.lastname: Cuenca
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism*; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis*; D053148:Caspase 3 / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D053148:Caspase 3