DARPP-32 binds to tra2-beta1 and influences alternative splicing.
The majority of human genes undergo alternative splicing, which is frequently altered in response to physiological stimuli. DARPP-32 (dopamine and cAMP regulated phosphoprotein, 32kDa) is a component of PKA-dependent signaling pathways. Here we show that DARPP-32 binds directly to the splicing factor tra2-beta1 (transformer 2). DARPP-32 changes the usage of tra2-beta1 dependent alternative exons in a concentration-dependent manner, suggesting that the DARPP-32:tra2-beta1 interaction is a molecular link between signaling pathways and pre-mRNA processing.
Benderska
Natalya
N
University of Erlangen-Nuremberg, Institute for Biochemistry, Fahrstrasse 17, 91054 Erlangen, Germany.
Becker
Kristina
K
Girault
Jean-Antoine
JA
Becker
Cord-Michael
CM
Andreadis
Athena
A
Stamm
Stefan
S
D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005091:Exons; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding; D054645:Protein Phosphatase 1 / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D000068103:Serine-Arginine Splicing Factors; D015398:Signal Transduction
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; C497928:PPP1R1B protein, human; D012322:RNA Precursors; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C097083:TRA2B protein, human; D000068103:Serine-Arginine Splicing Factors; D054645:Protein Phosphatase 1