[The effects of dendritic cells overexpressing Serrate1 on the differentiation of CD4(+)CD25(+) T cells in asthmatic mice].
OBJECTIVE
To observe the effects of dendritic cells (DCs) overexpressing Serrate1 on the differentiation of CD(4)(+)CD(25)(+) T cells and the production of inhibitory cytokines in asthmatic mice.


METHODS
Asthma mouse model was established by the routine method. After intravenous injection of the DCs transfected with Serrate1 into the naïve mice, the airway and lung inflammation was observed and the count of CD(4)(+)CD(25)(+) T cells delivered from the spleen and the percentage accounting for CD(4)(+)T cells were measured by flow cytometry. The expression of IL-10, transforming growth factor (TGF)beta1, and cytologic T-lymphocyte-associated antigen (CTLA)-4 mRNA of CD(4)(+) T cells were detected by RT-PCR.


RESULTS
Compared with the non-transfected group, the airway inflammation of asthmatic mice injected the DCs transfected with Serrate1 reduced significantly, the number of CD(4)(+)CD(25)(+) T cells delivered from the spleen and the percentage accounting for CD(4)(+) T cells and the expression of IL-10, TGFbeta1, and CTLA-4 mRNA of CD(4)(+) T increased.


CONCLUSIONS
DCs overexpressing Serrate1 could induce the differentiation of the CD(4)(+)CD(25)(+) T cells and increase the production of inhibitory cytokines in asthma in vivo, indicating that Notch1/Serrate1 signal pathway of DCs plays an important role in the induction of immune tolerance of T cells to allergens.
Sun
Kun
K
Institute of Respiratory Disease of PLA, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China.
Lin
Ke-xiong
KX
Wang
Chang-zheng
CZ
Wu
Kui
K
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D060908:CTLA-4 Antigen; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D000072099:Serrate-Jagged Proteins; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D015703:Antigens, CD; D060908:CTLA-4 Antigen; D002135:Calcium-Binding Proteins; C556707:Ctla4 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10