title: Interaction of heparin with cationic molecular probes: probe charge is a major determinant of binding stoichiometry and affinity.
abstract: Fluorescent perylenediimide probes modified with 2, 4, 6, or 8 ammonium groups were synthesized and their binding to the antithrombotic drug heparin was studied by fluorescence spectroscopy in solution. The polyanionic polysaccharide strands of heparin bind more probe molecules per sugar unit when the charge of the latter is low, and stability of the probe-heparin complex increases with increasing probe charge.
authors.affiliation: University of Heidelberg, Inorganic Chemistry Institute, 69120 Heidelberg, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Szelke
authors.forename: Sarah
authors.initials: S
authors.lastname: Schübel
authors.forename: Job
authors.initials: J
authors.lastname: Harenberg
authors.forename: Roland
authors.initials: R
authors.lastname: Krämer
mesh_terms: D001665:Binding Sites; D002240:Carbohydrate Sequence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D007094:Imides / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007094:Imides; D015335:Molecular Probes; C521332:perylenediimide; D010569:Perylene; D006493:Heparin