mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are altered in brains from subjects with autism.
We have shown altered expression of gamma-aminobutyric acid A (GABA(A)) and gamma-aminobutyric acid B (GABA(B)) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA(A) subunits previously associated with autism (GABRalpha4; GABRalpha5; GABRbeta1). Three GABA receptor subunits demonstrated mRNA and protein level concordance in superior frontal cortex (GABRalpha4, GABRalpha5, GABRbeta1) and one demonstrated concordance in cerebellum (GABBetaR1). These results provide further evidence of impairment of GABAergic signaling in autism.
Fatemi
S Hossein
SH
Department of Psychiatry, Division of Neuroscience Research, University of Minnesota School of Medicine, 420 Delaware St SE MMC 392, Minneapolis, MN 55455, USA. fatem002@umn.edu
Reutiman
Teri J
TJ
Folsom
Timothy D
TD
Rooney
Robert J
RJ
Patel
Diven H
DH
Thuras
Paul D
PD
D000328:Adult; D001321:Autistic Disorder / Q000378:metabolism*; D015153:Blotting, Western; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D011963:Receptors, GABA-A / Q000032:analysis*; D018080:Receptors, GABA-B / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
C546260:GABRA4 protein, human; C527986:GABRA5 protein, human; C546264:GABRB1 protein, human; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B