title: Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.).
abstract: The fatty acid profile of vegetable oils (VOs), together with the poor ability of marine fish to convert polyunsaturated fatty acids (PUFA) to highly unsaturated fatty acids (HUFA), lead to important changes in the nutritional value of farmed fish fed VO, which include increased fat and 18:2n-6 and reduced n-3 HUFA. Echium oil (EO) has a good n-3/n-6 balance as well as an interesting profile with its high content of unusual fatty acids (SDA, 18:4n-3 and GLA, 18:3n-6) that are of increasing pharmacological interest. The effects of substituting 50 % of dietary fish oil (FO) by EO on gilthead seabream (Sparus aurata L.) enterocyte and hepatocyte lipid metabolism were studied. After 4months of feeding, cell viability, total lipid contents and lipid class compositions were not affected by EO. The cells clearly reflected the fatty acid profile of the EO showing increased SDA, GLA and its elongation product 20:3n-6, and only minorly decreased n-3 HUFA compared to other VO. Metabolism of [1-(14)C]18:2n-6 and [1-(14)C]18:3n-3 was also unaffected by EO in terms of total uptake, incorporation, beta-oxidation and elongation-desaturation activities.
authors.affiliation: Departamento de Biología Animal, Universidad de La Laguna, Spain. mercidl81@hotmail.com
authors.forename: Mercedes
authors.initials: M
authors.lastname: Díaz-López
authors.forename: M José
authors.initials: MJ
authors.lastname: Pérez
authors.forename: Nieves Guadalupe
authors.initials: NG
authors.lastname: Acosta
authors.forename: Salvador
authors.initials: S
authors.lastname: Jerez
authors.forename: Roberto
authors.initials: R
authors.lastname: Dorta-Guerra
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Tocher
authors.forename: Antonio
authors.initials: A
authors.lastname: Lorenzo
authors.forename: Covadonga
authors.initials: C
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D036661:Echium / Q000737:chemistry*; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D050356:Lipid Metabolism; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D021541:Sea Bream / Q000378:metabolism*
chemical_list: D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D010938:Plant Oils