title: [Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells].
abstract: OBJECTIVE
  To study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.
  
  
  METHODS
  HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.
  
  
  RESULTS
  HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.
  
  
  CONCLUSIONS
  Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatology Hospital, The Affiliated Stomatology Hospital of Southern Medical University, Guangzhou 510280, China.
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Wu
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Zheng
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Zeng
authors.forename: Shao-Peng
authors.initials: SP
authors.lastname: Chen
authors.forename: Guo-Chu
authors.initials: GC
authors.lastname: Xue
authors.forename: Jin-Cai
authors.initials: JC
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D066255:EGF Family of Proteins; D042783:Endothelial Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007136:Immunoglobulins; D053634:TYK2 Kinase; D014471:Umbilical Veins
chemical_list: D066255:EGF Family of Proteins; D007136:Immunoglobulins; D053634:TYK2 Kinase