Inhibitory effect of anticholinergics on the contraction of isolated caprine urinary bladder detrusor muscle.
This study investigated whether four anticholinergics which are not clinically used for relaxing the urinary bladder detrusor muscle inhibit the contraction of isolated caprine (goat) detrusor muscle: cyclopentolate (100 nm), homatropine (5 microm), ipratropium (500 nm) and valethamate (1 microm). The effects of these anticholinergics were compared with tolterodine (3 microm), an anticholinergic clinically used for relaxing the detrusor muscle. The inhibitory effect of each of these five anticholinergics was studied on six strips of caprine detrusor muscle made to contract with 100 mum acetylcholine (ACh) by determining the percent inhibition of height of contraction and the area under the contractile curve (AUC). It was found that all five anticholinergics inhibited the ACh-induced contraction of the caprine detrusor and that this inhibition was reversed by raising the concentration of ACh. Hence, these four anticholinergics, like tolterodine, may be useful in managing clinical conditions that require relaxation of the detrusor muscle.
George
N
N
Department of Pharmacology, Christian Medical College, Vellore 632002, India.
Shiny
P J
PJ
Miriam
J
J
Nancy
C A
CA
Dhanasekar
K R
KR
Peedicayil
J
J
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D019540:Area Under Curve; D001559:Benzhydryl Compounds / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D003408:Cresols / Q000494:pharmacology; D003519:Cyclopentolate / Q000494:pharmacology; D006041:Goats; D066298:In Vitro Techniques; D009241:Ipratropium / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology*; D010665:Phenylpropanolamine / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D000068737:Tolterodine Tartrate; D014326:Tropanes / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects*
D001559:Benzhydryl Compounds; D018680:Cholinergic Antagonists; D003408:Cresols; D000644:Quaternary Ammonium Compounds; D014326:Tropanes; D010665:Phenylpropanolamine; D000068737:Tolterodine Tartrate; C100236:homatropine; C004904:valethamate; D009241:Ipratropium; D003519:Cyclopentolate; D000109:Acetylcholine