The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair.
The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.
Takahashi
Naoki
N
Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Gent, Belgium.
Quimbaya
Mauricio
M
Schubert
Veit
V
Lammens
Tim
T
Vandepoele
Klaas
K
Schubert
Ingo
I
Matsui
Minami
M
Inzé
Dirk
D
Berx
Geert
G
De Veylder
Lieven
L
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004260:DNA Repair; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012854:Sister Chromatid Exchange
D048868:Adaptor Proteins, Signal Transducing; D029681:Arabidopsis Proteins; C547652:CTF18 protein, Arabidopsis; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C530189:ETG1 protein, Arabidopsis; C519407:MCMBP protein, human; D009687:Nuclear Proteins