title: Ca2+ signaling induced by sphingosine 1-phosphate and lysophosphatidic acid in mouse B cells.
abstract: Lysophospholipids (LPLs) such as lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are chemotactic for lymphocytes, and increases of in cytosolic [Ca(2+)] signal the regulation of lymphocyte activation and migration. Here, the authors investigated the effects of LPA and S1P on [Ca(2+)](c) in mouse B cell lines (WEHI-231 and Bal-17) and primary B cells isolated from mouse spleen and bone marrow, and focused on the modulation of store-operated Ca(2+) entry (SOCE) by LPLs. In Bal-17 (a mature B cell line) both LPA and S1P induced a transient [Ca(2+)](c) increase via a phospholipase C pathway. In addition, pretreatment with LPLs was found to augment thapsigargin-induced SOCE in Bal-17 cells. However, in WEHI-231 (an immature B cell line) LPLs had no significant effect on [Ca(2+)](c) or SOCE. Furthermore, in freshly isolated splenic B cells (SBCs) and bone marrow B cells (BMBCs), LPLs induced only a small increase in [Ca(2+)](c). Interestingly, however, pretreatment with LPLs markedly increased SOCE in primary B cells, and this augmentation was more prominent in BMBCs than SBCs. The unidirectional influx of Ca(2+) was measured using Ba(2+) as a surrogate ion. Similarly, Ba(2+) influx was also found to be markedly increased by LPLs in SBCs and BMBCs. Summarizing, LPLs were found to strongly augment SOCE-mediated Ca(2+)-signaling in mouse B cells. However, unlike the mature Bal-17 cell line, PLC-dependent Ca(2+) release was insignificant in primary B cells and inWEHI-231.
authors.affiliation: Department of Physiology, Seoul National University College of Medicine, Seoul, 110-799, Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Nam
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Shin
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Min
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Ye
authors.forename: Ju-Hong
authors.initials: JH
authors.lastname: Jeon
authors.forename: Sung Joon
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D020013:Calcium Signaling / Q000187:drug effects / Q000276:immunology; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018929:Cell Culture Techniques; D002460:Cell Line; D050356:Lipid Metabolism / Q000276:immunology; D008213:Lymphocyte Activation; D038041:Lymphopoiesis; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D054448:Precursor Cells, B-Lymphoid / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013154:Spleen / Q000473:pathology; D019284:Thapsigargin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D019284:Thapsigargin; D010738:Type C Phospholipases; D000252:Calcium-Transporting ATPases; D013110:Sphingosine; C032881:lysophosphatidic acid