Use of a novel assay to evaluate enzymes that produce hydrogen sulfide in Fusobacterium nucleatum.
To evaluate enzymes that produce hydrogen sulfide (H(2)S) in species of Fusobacterium nucleatum, we developed an assay based on SDS-polyacrylamide gel electrophoresis with renaturation followed by active staining. This assay provided precise insight into the enzymes that produce H(2)S in terms of their number and molecular weights.
Yoshida
Yasuo
Y
Department of Pathogenesis and Control of Oral Disease, Iwate Medical University School of Dentistry, Morioka, Iwate, Japan. yasuoy@iwate-med.ac.jp
Ito
Shuntaro
S
Tamura
Haruki
H
Kunimatsu
Kazushi
K
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005674:Fusobacterium Infections / Q000150:complications / Q000201:enzymology* / Q000382:microbiology*; D016967:Fusobacterium nucleatum / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008970:Molecular Weight; D010510:Periodontal Diseases / Q000201:enzymology / Q000209:etiology; D013045:Species Specificity; D013194:Staining and Labeling
D006862:Hydrogen Sulfide