title: Functional activation of macrophages, monocytes and splenic lymphocytes by polysaccharide fraction from Tricholoma matsutake.
abstract: Mushroom-derived polysaccharides (beta-glucans) are considered as a valuable biopharmaceutical principle without displaying side effects. Although Tricholoma matsutake is well-known mushroom in Korea, Japan and China, the immunoregulatory roles of T. matsutake-derived polysaccharides were not fully elucidated yet. In this study, we continued to evaluate the immunomodulatory effect of T. matsutake-derived polysaccharide fraction (TmC-2) using functional activation models of macrophages, monocytes and splenic lymphocytes. TmC-2 treatment strongly increased the production of NO and TNF-alpha. Phagocytic uptake and ROS generation was also up-regulated by TmC-2. Interestingly, TmC-2 stimulated CD29-mediated cell-cell or cell-finbronectin adhesions in monocytes, while CD43-mediated cell adhesion was down-regulated. Interestingly, the enhancement of proliferation and IFN-gamma production was striking observed in TmC-2-treated splenic lymphocytes. The activation seemed to be mediated by up-regulating intracellular signaling cascades such as PI3K/Akt and MAPK (ERK and p38) and by the involvement of surface receptors (dectin-1 and TLR-2). Therefore, our results suggest that this TmC-2 from T. matsutake can be developed as a promising immunostimulatory principle, applicable to people with lowered immunomodulatory potentials.
authors.affiliation: School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-707, Korea.
authors.forename: Se Eun
authors.initials: SE
authors.lastname: Byeon
authors.forename: Jaehwi
authors.initials: J
authors.lastname: Lee
authors.forename: Eunji
authors.initials: E
authors.lastname: Lee
authors.forename: Song Yi
authors.initials: SY
authors.lastname: Lee
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
authors.forename: Young Eon
authors.initials: YE
authors.lastname: Kim
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D008518:Medicine, East Asian Traditional; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000276:immunology / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D055439:Tricholoma / Q000737:chemistry*; D020298:U937 Cells; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D007371:Interferon-gamma