title: Electrochemistry and electrocatalytic of hemoglobin immobilized on FDU-15-Pt mesoporous materials.
abstract: In this paper, the composite films of Nafion/Hb/FDU-Pt-15/PDDA were constructed by layer-by-layer assembly technique. The FDU-15-Pt mesoporous materials were synthesized and characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The resulting films were verified by electrochemical impedance spectroscopy (EIS), cyclic voltammetry and UV-vis spectroscopy. The direct electrochemical and electrocatalytic properties of hemoglobin (Hb) in the Nafion/Hb/FDU-15-Pt/PDDA films were also investigated. A pair of stable and well-defined reversible redox peaks was observed and the formal potential of Hb Fe(III)/Fe(II) redox couple was found to be -0.324 V (vs. SCE). Based on a series of optimized conditions, the films modified electrode displayed a good electrocatalytic activity to the reduction of hydrogen peroxide (H2O2), which had linear current response from 2.0 x 10(-6) M to 6.0 x 10(-2) M with the detection limit of 1.0 x 10(-6) M (S/N=3). The apparent Michaelis-Menten constant (K(m)(app)) was determined to be 0.15 mM in the range of lower concentration of H2O2. The apparent heterogeneous electron transfer rate constant (k(s)) was 1.05 +/- 0.03 s(-1). The good sensitivity, reproducibility, excellent stability and wide linear range make Nafion/Hb/FDU-15-Pt/PDDA a promising application of the preparation of third-generation biosensor.
authors.affiliation: Department of Chemistry and Shanghai Key Laboratory of Green Chemistry and Chemical Process, East China Normal University, 3663 Zhongshan Road(N), Shanghai 200062, PR China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Nie
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhou
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D055162:Biocatalysis; D004563:Electrochemistry; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D005465:Fluorocarbon Polymers / Q000737:chemistry / Q000378:metabolism; D005468:Fluorodeoxyuridylate / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D005465:Fluorocarbon Polymers; D006454:Hemoglobins; D055767:Immobilized Proteins; D009942:Organometallic Compounds; D005468:Fluorodeoxyuridylate; C040402:perfluorosulfonic acid; D010984:Platinum; D006861:Hydrogen Peroxide