[CD-34 and CASPASE-3 biomarkers expression in esophageal epidermoid carcinoma].
OBJECTIVE
To analyze by cytophotometry study the expression of CD-34 angiogenesis and caspase-3 apoptosis markers in the esophagus squamous cell carcinoma and to correlate markers themselves.


METHODS
Twenty-nine esophageal squamous cell carcinoma specimens were submitted to immunohystochemical analysis, based on label index of CD-34 and caspase-3 antibodies, using cytophotometry computer system. Variables considered in the correlation analysis were biomarkers expression quantification, patient age, lesion size, and TNM classification system.


RESULTS
Index label median value for CD-34 was 72,6% and for caspase-3 represented 96,5%. Statistical significant correlation was not demonstrated between the respective biomarkers and lesion size (p=0,96) or patient age (p=0,95). There was a slightly tendency to a positive correlation between CD-34 index label and TNM classification system (p=0,11). Although presenting a higher index label than CD-34 marker (p=0,007), caspase-3 did not show any relationship with the studied variables (p>0,19). The correlation between CD-34 and caspase-3 presented a small positive tendency (r=0,09 and p=0,65).


CONCLUSIONS
Both studied biomarkers showed good expression in esophageal squamous cell carcinoma, whereas CD-34 marker presented lower expression than caspase-3 in this specific tumor. The respective biomarkers do not demonstrate any correlation between themselves.
Ferreira
Valério Alves
VA
Programa de Pós-graduação, Faculdade Evangélica do Paraná, PR, BR. ipem@evangelico.org.br
Czeczko
Nicolau Gregori
NG
Ribas-Filho
Jurandir Marcondes
JM
Malafaia
Osvaldo
O
Ribas
Carmen Austrália Paredes Marcondes
CA
Gomes
Claudia Stein
CS
Cunha
Regina Maria da
RM
Cuenca
Ronaldo M
RM
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism*; D053148:Caspase 3 / Q000032:analysis / Q000096:biosynthesis*; D004938:Esophageal Neoplasms / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged
D018952:Antigens, CD34; D015415:Biomarkers; D053148:Caspase 3