title: Serotyping of naturally Toxoplasma gondii infected meat-producing animals.
abstract: Serotyping was previously described as a promising method for typing strains of Toxoplasma gondii. The majority of precedent studies utilized serum samples collected from human patients with different T. gondii-associated pathologies. The aim of this work was to study the applicability of the same procedure for serotyping naturally infected meat-producing animals. An ELISA test based on GRA6 and GRA7 C-terminal polymorphic peptides was used. Peptide GRA6II has polymorphisms specific for the archetypal strains type II, GRA6I/III for strains type I and III, GRA7I for strains type I and GRA7III for strains type III. As reference material, and to validate this approach, serum samples from eleven free-range chickens and fifteen pigs used for Toxoplasma genotypes isolation were selected. These strains integrate the Biological Resource Centre (BRC) ToxoBS Bank. Three serum samples from chickens and two from pigs had serotyping results in agreement with genotyping. Thirty-five serum samples from chickens, twenty-nine from pigs and fifty from sheep, seropositive for T. gondii, from which no isolate was obtained, were also serotyped. Serotype III appeared significantly more frequent among sheep. Our results show that serotyping still need refinement, but may become a valuable tool for typing Toxoplasma strains from animal origin.
authors.affiliation: Centro de Imunologia e Biologia Parasitária (CIBP), CSPGF, INSA Porto, Portugal. sanrspt@yahoo.co.uk
authors.forename: Susana
authors.initials: S
authors.lastname: Sousa
authors.forename: Nuno
authors.initials: N
authors.lastname: Canada
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Correia da Costa
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Dardé
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016266:Food Parasitology; D005838:Genotype; D008460:Meat / Q000469:parasitology*; D011174:Portugal; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012703:Serotyping / Q000662:veterinary; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics; D014123:Toxoplasmosis / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C095047:GRA6 protein, Toxoplasma gondii; C112573:GRA7 protein, Toxoplasma gondii; D015800:Protozoan Proteins