In vivo endoscopic multi-beam optical coherence tomography.
A multichannel optical coherence tomography (multi-beam OCT) system and an in vivo endoscopic imaging probe were developed using a swept-source OCT system. The distal optics were micro-machined to produce a high numerical aperture, multi-focus fibre optic array. This combination resulted in a transverse design resolution of <10 microm full width half maximum (FWHM) throughout the entire imaging range, while also increasing the signal intensity within the focus of the individual channels. The system was used in a pre-clinical rabbit study to acquire in vivo structural images of the colon and ex vivo images of the oesophagus and trachea. A good correlation between the structural multi-beam OCT images and H&E histology was achieved, demonstrating the feasibility of this high-resolution system and its potential for in vivo human endoscopic imaging.
Standish
Beau A
BA
Deptartment of Medical Biophysics, University of Toronto, Toronto, Canada. standish@ee.ryerson.ca
Lee
Kenneth K C
KK
Mariampillai
Adrian
A
Munce
Nigel R
NR
Leung
Michael K K
MK
Yang
Victor X D
VX
Vitkin
I Alex
IA
D000818:Animals; D003106:Colon / Q000033:anatomy & histology; D020685:Colonoscopes; D019723:Endoscopes; D004867:Equipment Design; D004947:Esophagus / Q000033:anatomy & histology; D005240:Feasibility Studies; D005336:Fiber Optic Technology / Q000295:instrumentation; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009262:Nails / Q000033:anatomy & histology; D011817:Rabbits; D041623:Tomography, Optical Coherence / Q000295:instrumentation*; D014132:Trachea / Q000033:anatomy & histology