title: The role of LTA4H and ALOX5AP genes in the risk for asthma in Latinos.
abstract: BACKGROUND
  Leukotrienes play an important role in allergic and inflammatory diseases, but reports on the involvement of arachidonate 5-lipoxygenase-activating protein (ALOX5AP) and leukotriene A(4) hydrolase (LTA4H) in asthma have been inconclusive.
  
  
  OBJECTIVE
  To determine whether polymorphisms in ALOX5AP and LTA4H genes are risk factors for asthma in two different Latino groups: Mexicans and Puerto Ricans.
  
  
  METHODS
  The LTA4H gene was sequenced in individuals from both groups to identify novel polymorphisms. Single-nucleotide polymorphisms (SNPs) in the ALOX5AP and LTA4H genes were analysed for associations with asthma and asthma-related phenotypes in 687 parent-child trios of Mexican and Puerto Rican origin.
  
  
  RESULTS
  In LTA4H, five previously unknown polymorphisms were identified. Two SNPs within LTA4H (rs17525488 and rs2540493) were protective for asthma in Latinos (P=0.007 and 0.05, respectively). Among the Mexican patients, LTA4H polymorphisms were associated with baseline lung function and IgE levels. For ALOX5AP, the minor allele at SNP rs10507391 was associated with protection from asthma (odds ratio=0.78, P=0.02) and baseline lung function (P=0.018) in Puerto Ricans. A gene-gene interaction was identified between LTA4H (rs17525488) and ALOX5AP (rs10507391), (P=0.003, in the combined sample).
  
  
  CONCLUSIONS
  Our results support the role of LTA4H and ALOX5AP variants as risk factors for asthma in Latino populations.
authors.affiliation: Lung Biology Center, Department of Medicine, Case Western Reserve University, Cleveland, OH, USA. marc.viagarcia@ucsf.edu <marc.viagarcia@ucsf.edu>
authors.forename: M
authors.initials: M
authors.lastname: Via
authors.forename: A
authors.initials: A
authors.lastname: De Giacomo
authors.forename: H
authors.initials: H
authors.lastname: Corvol
authors.forename: C
authors.initials: C
authors.lastname: Eng
authors.forename: M A
authors.initials: MA
authors.lastname: Seibold
authors.forename: C
authors.initials: C
authors.lastname: Gillett
authors.forename: J
authors.initials: J
authors.lastname: Galanter
authors.forename: S
authors.initials: S
authors.lastname: Sen
authors.forename: H
authors.initials: H
authors.lastname: Tcheurekdjian
authors.forename: R
authors.initials: R
authors.lastname: Chapela
authors.forename: J R
authors.initials: JR
authors.lastname: Rodríguez-Santana
authors.forename: W
authors.initials: W
authors.lastname: Rodríguez-Cintrón
authors.forename: S
authors.initials: S
authors.lastname: Thyne
authors.forename: P C
authors.initials: PC
authors.lastname: Avila
authors.forename: S
authors.initials: S
authors.lastname: Choudhry
authors.forename: E
authors.initials: E
authors.lastname: González Burchard
mesh_terms: D058927:5-Lipoxygenase-Activating Proteins; D000293:Adolescent; D000483:Alleles; D001249:Asthma / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002648:Child; D004851:Epoxide Hydrolases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006630:Hispanic or Latino / Q000235:genetics*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D017058:Mexican Americans; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D002352:Carrier Proteins; D008565:Membrane Proteins; D004851:Epoxide Hydrolases; C043134:leukotriene A4 hydrolase