title: Effect of topical mitomycin-C on total collagen deposits on the submucosa of intact vocal folds in swine.
abstract: OBJECTIVE
  To compare the effects of topical mitomycin-C at different concentrations on submucosal collagen deposition on the vocal folds of swine.
  
  
  METHODS
  The animals were divided into three groups according to the composition of the topical solution to be applied to the vocal folds: 0.9% saline solution (control group); 4 mg/ml mitomycin-C (group 1) and 8 mg/ml mitomycin-C (group 2). Thirty days after the application, all animals were sacrificed, their vocal folds were collected and stained by the picrosirius red technique, and submucosal collagen deposition areas were estimated by the Image Pro Plus 4.5 software. Mann-Whitney test was used to compare differences between parameters of each group.
  
  
  RESULTS
  The means of the areas of submucosal collagen deposits on vocal folds were 3110.44 square micrometers (microm(2)), 3115.98 microm(2) and 3105.78 microm(2) for groups control, 1 and 2, respectively. There were no statistical differences across the three groups (p>0.05).
  
  
  CONCLUSIONS
  Mitomycin-C topically applied to intact vocal folds of swine did not alter submucosal collagen deposition.
authors.affiliation: Graduate Program in Clinics and Surgery, Universidade Federal do Paraná - UFPR.
authors.forename: Marcelo Charles
authors.initials: MC
authors.lastname: Pereira
authors.forename: Carlos Domingues
authors.initials: CD
authors.lastname: Repka
authors.forename: Paulo Antonio Monteiro
authors.initials: PA
authors.lastname: Camargo
authors.forename: Daniel Zeni
authors.initials: DZ
authors.lastname: Rispoli
authors.forename: Antônio Carlos Ligocki
authors.initials: AC
authors.lastname: Campos
authors.forename: Jorge Eduardo Fouto
authors.initials: JE
authors.lastname: Matias
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000187:drug effects* / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage* / Q000494:pharmacology; D009092:Mucous Membrane / Q000378:metabolism; D013552:Swine; D014827:Vocal Cords / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D016685:Mitomycin; D003094:Collagen