title: Maintenance of previously uncultured freshwater archaea from anoxic waters under laboratory conditions.
abstract: Culture conditions for the maintenance of previously uncultured members of the Archaea thriving in anoxic water layers of stratified freshwater lakes are described. The proposed enrichment conditions, based on the use of defined medium composition and the maintenance of anoxia, have been proven effective for the maintenance of the archaeal community with virtually no changes over time for periods up to 6 months as revealed by a PCR-DGGE analysis. Phylotypes belonging to groups poorly represented in culture collections such as the Deep-Sea Hydrothermal Vent Euryarchaeota (DHVE) and the Miscellaneous Crenarchaeotic Group (MCG) were maintained and selectively enriched when compared to the correspondent indigenous planktonic archaeal community.
authors.affiliation: Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Campus Montilivi, Girona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Plasencia
authors.forename: Lluís
authors.initials: L
authors.lastname: Bañeras
authors.forename: Marc
authors.initials: M
authors.lastname: Llirós
authors.forename: Emilio O
authors.initials: EO
authors.lastname: Casamayor
authors.forename: Carles
authors.initials: C
authors.lastname: Borrego
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D016172:DNA Fingerprinting; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005618:Fresh Water / Q000382:microbiology*; D000860:Hypoxia; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S