title: A comparison amongst three rapid urease tests to diagnose Helicobacter pylori infection in 375 consecutive dyspeptic.
abstract: Rapid diagnostic tools for Helicobacter pylori are important in endoscopy. To assess the accuracy of a new 5-min rapid urease test (UFT300, ABS Cernusco, sul Naviglio, Italy) compared with the 1-h Pyloritek (Serim Laboratories, Elkhart, IN) and the 24-h CLO test (Kimberly-Clark Ballard Medical Products, Roswell, GA), consecutive dyspeptic patients referred to our unit for endoscopy were prospectively studied. All patients underwent a 13C-urea-breath test, histology and the UFT300 (ABS; Cernusco, sul Naviglio, Italy). Two additional rapid urease tests were performed. Patients were deemed infected when both 13C-UBT and histology were positive. Rapid urease tests were read at 1, 5, and 60 min, respectively. Of the 375 enrolled patients, 45.3% were infected with H. pylori. The sensitivity of the new 5-min rapid urease test 300 was 90.3, 94.5, and 96.2% at 1, 5, and 60 min, respectively (specificity 100%). The Pyloritek and the new 5-min rapid urease test were comparable, but the CLO test was not reliable at 5 and 60 min. In conclusion, the new 5-min rapid urease test is comparable to the Pyloritek test, but the CLO test is significantly less sensitive at early time points. Reading the test results at 1 min may increase false-negative results with decreasing sensitivity.
authors.affiliation: Department of Internal Medicine and Gastroenterology, S. Orsola Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. berardino.vaira@unibo.it
authors.forename: Dino
authors.initials: D
authors.lastname: Vaira
authors.forename: Luigi
authors.initials: L
authors.lastname: Gatta
authors.forename: Chiara
authors.initials: C
authors.lastname: Ricci
authors.forename: Federico
authors.initials: F
authors.lastname: Perna
authors.forename: Ilaria
authors.initials: I
authors.lastname: Saracino
authors.forename: Giulia
authors.initials: G
authors.lastname: Fiorini
authors.forename: Valentina
authors.initials: V
authors.lastname: Castelli
authors.forename: John
authors.initials: J
authors.lastname: Holton
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests; D004415:Dyspepsia / Q000382:microbiology*; D005188:False Negative Reactions; D005260:Female; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014508:Urea / Q000032:analysis; D014510:Urease / Q000032:analysis*
chemical_list: D011933:Reagent Kits, Diagnostic; D014508:Urea; D014510:Urease