FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium.
During the development of the vertebrate nervous system, mitosis of neural progenitor cells takes place near the lumen, the apical side of the neural tube, through a characteristic movement of nuclei known as interkinetic nuclear migration (INM). Furthermore, during the proliferative period, neural progenitor cells exhibit planar cell divisions to produce equivalent daughter cells. Here, we examine the potential role of extracellular signals in INM and planar divisions using the medaka mutant tacobo (tab). This tab mutant shows pleiotropic phenotypes, including neurogenesis, and positional cloning identified tab as laminin gamma1 (lamc1), providing a unique framework to study the role of extracellular signals in neurogenesis. In tab mutant neural tubes, a number of nuclei exhibit abnormal patterns of migration leading to basally mislocalized mitosis. Furthermore, the orientation of cell division near the apical surface is randomized. Probably because of these defects, neurogenesis is accelerated in the tab neural tube. Detailed analyses demonstrate that extracellular signals mediated by the FAK pathway regulate INM and planar divisions in the neuroepithelium, possibly through interaction with the intracellular dynein-motor system.
Tsuda
Sachiko
S
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Kitagawa
Tadao
T
Takashima
Shigeo
S
Asakawa
Shuichi
S
Shimizu
Nobuyoshi
N
Mitani
Hiroshi
H
Shima
Akihiro
A
Tsutsumi
Makiko
M
Hori
Hiroshi
H
Naruse
Kiyoshi
K
Ishikawa
Yuji
Y
Takeda
Hiroyuki
H
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D046569:Neuroepithelial Cells / Q000166:cytology / Q000378:metabolism*; D009990:Oryzias; D015398:Signal Transduction / Q000502:physiology*
D029941:Fish Proteins