Histological evaluation of periodontal regeneration in autogenous tooth transplantation in the dog: a comparison between one and two-stage surgical techniques, a pilot study.
OBJECTIVE
To compare the periodontal regeneration, associated with autogenous tooth transplantation in dogs, using either one- or two-stage surgical techniques.


METHODS
The study group consisted of three Beagles, older than 5 months, in which six incisors and six premolars were transplanted to mechanically prepared recipient sockets. One group was transplanted using a one-stage method to recipient beds prepared immediately before transplantation. The second group of teeth were transplanted using a two-stage method in which the recipient beds were prepared and left to heal for 7 days before transplantation. Clinical examinations were done every week and the animals were euthanized 9 weeks later. Subsequently, decalcified sections were prepared for routine and immunohistochemical histological evaluation. Periodontal healing was evaluated by undertaking histomorphometric analysis and analyzed using the Mann-Whitney test (P = 0.05).


RESULTS
All the transplanted teeth in both groups survived. No statistically significant difference was found in the complete healing between the treatment groups (P = 0.053). There was no difference between the occurrence of inflammatory root resorption (P = 0.135) and replacement root resorption (P = 0.081).


CONCLUSIONS
This study demonstrated that there was no difference between the two surgical techniques in terms of preventing root resorption in transplanted teeth.
Marques
Ferreira Manuel
FM
Department of Dentistry, University School of Medicine, Coimbra, Portugal. m.mferreira@netcabo.pt
Filomena
Botelho Maria
BM
Lina
Carvalho
C
Barbara
Oliveiros
O
Palmeirão
Carrilho Eunice Virgínia
CE
D000818:Animals; D001641:Bicuspid / Q000637:transplantation*; D001861:Bone Regeneration; D004285:Dogs; D007180:Incisor / Q000637:transplantation*; D008297:Male; D019647:Oral Surgical Procedures / Q000009:adverse effects / Q000379:methods*; D010519:Periodontium / Q000502:physiology*; D010865:Pilot Projects; D012391:Root Resorption / Q000209:etiology / Q000517:prevention & control*; D020390:Tooth Socket / Q000601:surgery