title: Gas chromatography-mass spectrometry method for the determination of methadone and 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP).
abstract: Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, and wean heroin addicts. Measurement of methadone and its major metabolite EDDP in urine is useful for assessing compliance with addiction rehabilitation and pain management programs. This method quantitatively measures methadone and its metabolite EDDP in urine. Methadone and EDDP are recovered from urine by solid phase extraction at pH 6.0. Analysis is performed by gas chromatography with mass spectrometry detection, using selective ion monitoring.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Toxicology and Drug Monitoring Laboratory, Rochester, MN, USA.
authors.forename: Christine L H
authors.initials: CL
authors.lastname: Snozek
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Bjergum
authors.forename: Loralie J
authors.initials: LJ
authors.lastname: Langman
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008691:Methadone / Q000652:urine*; D011759:Pyrrolidines / Q000652:urine*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D011759:Pyrrolidines; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine