Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1.
The dengue virus (DENV) nonstructural protein 1 (NS1) is an immunogenic protein that holds potential for the development of vaccines and diagnostic reagents; however, the epitopes of NS1 have not been comprehensively mapped. We mapped B-cell linear epitopes on NS1 using 149 monoclonal antibodies with DENV serotype specificity and cross-reactivity as well as antisera from 27 mice immunized with the four DENV serotypes. Epitope recognition analysis was performed using a set of 15-mer sequential overlapping peptides that spanned the entire NS1 protein from DENV-1. This strategy identified three regions of NS1 that are DENV-1 serotype-specific epitopes, namely amino acid residues 1-15, 71-85, and 338-352. We also identified five group-specific B-cell epitopes that were highly conserved among isolates of the four DENV serotypes. These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays.
Chen
Yue
Y
Center for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, No. 253 Gong ye da dao zhong, Guangzhou 510282, People's Republic of China.
Pan
Yuxian
Y
Guo
Yonghui
Y
Qiu
Liwen
L
Ding
Xixia
X
Che
Xiaoyan
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D003429:Cross Reactions / Q000235:genetics / Q000276:immunology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D018985:Epitopes, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D016056:Immunodominant Epitopes / Q000235:genetics* / Q000276:immunology; D051379:Mice; D016415:Sequence Alignment; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes; D014764:Viral Proteins