Respiratory function and pulmonary lesions in pigs infected with porcine reproductive and respiratory syndrome virus.
Pulmonary dysfunction was evaluated in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV, isolate VR-2332) and compared to clinical and pathological findings. Infected pigs developed fever, reduced appetite, respiratory distress and dullness at 9 days post-inoculation (dpi). Non-invasive pulmonary function tests using impulse oscillometry and rebreathing of test gases (He, CO) revealed peripheral airway obstruction, reduced lung compliance and reduced lung CO-transfer factor. PRRSV-induced pulmonary dysfunction was most marked at 9-18 dpi and was accompanied by a significantly increased respiratory rate and decreased tidal volume. Expiration was affected more than inspiration. On histopathological examination, multifocal areas of interstitial pneumonia (more severe and extensive at 10 dpi than 21 dpi) were identified as a possible structural basis for reduced lung compliance and gas exchange disturbances.
Wagner
Judith
J
Institute of Molecular Pathogenesis in the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburger Str 96a, 07743 Jena, Germany.
Kneucker
Annette
A
Liebler-Tenorio
Elisabeth
E
Fachinger
Vicky
V
Glaser
Melanie
M
Pesch
Stefan
S
Murtaugh
Michael P
MP
Reinhold
Petra
P
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012129:Respiratory Function Tests / Q000662:veterinary; D013552:Swine