Antioxidant activity of biotransformed sex hormones facilitated by Bacillus stearothermophilus.
Bacillus stearothermophilus, a thermophilic bacterium isolated from Kuwaiti desert, when incubated with exogenous progesterone for 10 days at 65 degrees C produced two monohydroxylated, two dihydroxy isomers of progesterone and a B-Seco compound. These metabolites were purified by TLC and HPLC followed by their identification through (1)H, (13)C NMR and other spectroscopic data. Microbial hydroxylation of 17beta-estradiol resulted in the production of estrone. The effect of some inducers resulted in the production of two metabolites from 17beta-estradiol one of which was identified as 3,6beta,17beta-trihydroxyestra-1,3,5,14(10)-tetrene and the other metabolite remained unidentified. The transformation products were identified through their spectral data and comparison with reference to compounds. Antioxidant activities of progesterone transformed mixture and purified metabolites of 17beta-estradiol were studied by linoleic acid/beta-carotene assay. An enhanced antioxidant activity for progesterone transformation products was observed, when compared to progesterone. A comparison of antioxidant activity of progesterone and 17beta-estradiol transformation products is reported.
Afzal
Mohammad
M
Department of Biological Sciences, Faculty of Science, Kuwait University, Safat, Kuwait.
Al-Awadi
Sameera
S
Oommen
Sosamma
S
D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011374:Progesterone / Q000378:metabolism*
D000975:Antioxidants; D012739:Gonadal Steroid Hormones; D011374:Progesterone; D004958:Estradiol