title: 16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures.
abstract: A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.
authors.affiliation: Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark. ujustesen@health.sdu.dk
authors.forename: Ulrik Stenz
authors.initials: US
authors.lastname: Justesen
authors.forename: Marianne Nielsine
authors.initials: MN
authors.lastname: Skov
authors.forename: Elisa
authors.initials: E
authors.lastname: Knudsen
authors.forename: Hanne Marie
authors.initials: HM
authors.lastname: Holt
authors.forename: Per
authors.initials: P
authors.lastname: Søgaard
authors.forename: Tage
authors.initials: T
authors.lastname: Justesen
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S