title: Fasting increases tobramycin oral absorption in mice.
abstract: The pharmacokinetics of the aminoglycoside tobramycin was evaluated after oral administration to fed or fasting (15 h) mice. As expected, under normal feeding conditions, oral absorption was negligible; however, fasting induced a dramatic increase in tobramycin bioavailability. The dual-sugar test with lactulose and l-rhamnose confirmed increased small bowel permeability via the paracellular route in fasting animals. When experiments aimed at increasing the oral bioavailability of hydrophilic compounds are performed, timing of fasting should be extremely accurate.
authors.affiliation: Department of Reproductive and Developmental Sciences, University of Trieste, and Institute of Child Health IRCCS Burlo Garofolo, Trieste, Italy.
authors.forename: Luigina
authors.initials: L
authors.lastname: De Leo
authors.forename: Nicola
authors.initials: N
authors.lastname: Di Toro
authors.forename: Giuliana
authors.initials: G
authors.lastname: Decorti
authors.forename: Noelia
authors.initials: N
authors.lastname: Malusà
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ventura
authors.forename: Tarcisio
authors.initials: T
authors.lastname: Not
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D001682:Biological Availability; D005215:Fasting / Q000378:metabolism*; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007408:Intestinal Absorption; D007792:Lactulose / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012210:Rhamnose / Q000008:administration & dosage; D014031:Tobramycin / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D000900:Anti-Bacterial Agents; D007792:Lactulose; D012210:Rhamnose; D014031:Tobramycin