An in vitro intact globe expansion method for evaluation of cross-linking treatments.
OBJECTIVE
To measure the tissue mechanical response to elevated intraocular pressure (IOP) using intact globe expansion of rabbit eyes. This method examined rabbit kit (2-3 weeks old) eyes as a model for weakened tissue and evaluated riboflavin/UVA and glyceraldehyde cross-linking treatments.


METHODS
The ocular shape of enucleated eyes was photographed during a 24-hour period while a controlled IOP was imposed (either low IOP = 22 mm Hg or high IOP = 85 mm Hg). Untreated controls consisted of kit eyes tested at both low- and high IOP and adult eyes tested at high IOP. Treated kit eyes (dextran controls, riboflavin/UVA treatment of the cornea, and glyceraldehyde treatment of the entire globe) were tested at high IOP.


RESULTS
Low IOP elicited negligible creep of the sclera and very gradual creep of the cornea. In contrast, high IOP induced up to an 8% strain in the sclera and a 15% strain in the cornea of rabbit kit eyes. The expansion of adult eyes was less than one third that of kit eyes at the same, high IOP. Riboflavin/UVA treatment of corneas reduced expansion compared with that in both dextran-treated and untreated control corneas. Glyceraldehyde treatment prevented expansion of the cornea and sclera.


CONCLUSIONS
The intact globe expansion method (GEM) imposes a loading geometry comparable to in vivo conditions and can quantify changes in mechanical stability as a function of testing conditions (e.g., IOP, tissue maturation, and therapeutic cross-linking) with small sample sizes and small variability. Rabbit kit eyes provide a model of weak tissue suitable for screening treatments that strengthen the cornea and sclera.
Mattson
Matthew S
MS
Department of Engineering and Applied Science, California Institute of Technology, Pasadena, California 91125, USA.
Huynh
Joyce
J
Wiseman
Meredith
M
Coassin
Marco
M
Kornfield
Julia A
JA
Schwartz
Daniel M
DM
D000818:Animals; D000831:Animals, Newborn; D001696:Biomechanical Phenomena; D003094:Collagen / Q000378:metabolism*; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism; D004547:Elastic Tissue / Q000502:physiology; D005260:Female; D005985:Glyceraldehyde / Q000627:therapeutic use; D007429:Intraocular Pressure; D008297:Male; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011817:Rabbits; D012256:Riboflavin / Q000494:pharmacology*; D012590:Sclera / Q000187:drug effects* / Q000378:metabolism; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; D005985:Glyceraldehyde; D003094:Collagen; D012256:Riboflavin