title: [Inhibitory role of SirT7 in the growth of P19 cell line].
abstract: OBJECTIVE
  To study the regulatory role of SirT7, one of class histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19.
  
  
  METHODS
  We used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper.
  
  
  RESULTS
  Compared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line.
  
  
  CONCLUSIONS
  SirT7 play a dominant role in the grow inhibition of the P19 cells.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of National Laboratory of Medical Molecular Biology, Beijing 100005, China.
authors.forename: Jian-yi
authors.initials: JY
authors.lastname: Lu
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Shen
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids / Q000235:genetics; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: C539736:Sirt7 protein, mouse; D037761:Sirtuins