Determination of erythrocyte fragility as a marker of pesticide-induced membrane oxidative damage.
Erythrocytes are readily available cells and a good model system to study the health status of individuals with pathologic complications. It can also serve as a meaningful target to study toxicant/xenobiotic-induced damages. We have prepared different concentrations of a carbamate pesticide (carbofuran) and carried out experiments to determine its toxicity on erythrocytes in terms of mean erythrocyte fragility (MEF). We observed a significant alteration in the osmotic fragility upon treatment with carbofuran. In our earlier studies we have observed a good correlation between OF and OS in diabetic subjects. Study reveals OF as a potential biomarker of oxidative membrane damage in pathologic conditions as well as toxicant/xenobiotic/pesticide-induced oxidative membrane damage to erythrocytes.
Sharma
Bechan
B
Medicinal Research Lab, Drug Discovery & Development Division, Alternative Therapeutics Unit, Department of Chemistry, University of Allahabad, Allahabad, India. sharmabi@yahoo.com
Rai
Devendra K
DK
Rai
Prashant Kumar
PK
Rizvi
S I
SI
Watal
Geeta
G
D002219:Carbamates / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects*; D010084:Oxidation-Reduction / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*
D002219:Carbamates; D010575:Pesticides