TAT-peptide modified liposomes: preparation, characterization, and cellular interaction.
In general, cellular internalization of macromolecular drugs encapsulated in liposomes proceeds via endocytosis. This potentially leads to degradation of the liposome-encapsulated macromolecular content within the endosomal/lysosomal compartment. Therefore, bypassing the endocytic route by conferring a direct plasma membrane translocation property to the liposomes would be very beneficial. Cell penetrating peptides, e.g. TAT-peptide, are exploited in the drug delivery field for their capacity of plasma membrane translocation. Here, we describe the preparation of TAT-peptide modified liposomes and their cellular interaction using live cell flow cytometry and imaging techniques.
Fretz
Marjan M
MM
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Storm
Gert
G
D045744:Cell Line, Tumor; D004705:Endocytosis; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000493:pharmacokinetics*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000493:pharmacokinetics*
D008081:Liposomes; D054322:tat Gene Products, Human Immunodeficiency Virus