Analysis of supercoiled DNA by agarose gel electrophoresis using low-conducting sodium threonine medium.
We describe a new low-ionic-strength sodium threonine (STh) medium with the advantage of avoiding relative DNA band migration changes following electrophoresis of supercoiled DNA in agarose gel when substituted for the standard conductive medium of TBE (Tris-boric acid-ethylenediaminetetraacetic acid [EDTA]) or TAE (Tris-acetic acid-EDTA) or the low-ionic-strength sodium boric acid medium. Low-ionic-strength STh medium provided better resolution, less heat generation, and prevention of relative migration order changes among linear, covalently closed circular-, and open circular-formed DNA in the range of 2-10 kilobase pairs in 1% agarose gel electrophoresis.
Ishido
Tomomi
T
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa 761-0395, Japan.
Ishikawa
Mitsuru
M
Hirano
Ken
K
D001881:Borates / Q000737:chemistry; D004270:DNA, Circular / Q000032:analysis; D004278:DNA, Superhelical / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D009994:Osmolar Concentration; D013912:Threonine / Q000737:chemistry*
D001881:Borates; D004270:DNA, Circular; D004278:DNA, Superhelical; D013912:Threonine; C010634:sodium borate