Circulating vascular progenitor cells in patients with type 1 diabetes and microalbuminuria.
OBJECTIVE
Patients with type 1 diabetes and microalbuminuria are at increased risk of cardiovascular disease (CVD). Abnormalities in vascular progenitor cells, which participate in vascular repair, may be implicated in this susceptibility.


METHODS
We studied the number and function of vascular progenitor cells in 22 type 1 diabetic patients with history of microalbuminuria (MA(+)) and 22 type 1 diabetic patients without history of microalbuminuria (MA(-)), of similar age, diabetes duration, glycemic control, renal function, and no history of CVD.


RESULTS
MA(+) patients had lower circulating CD34(+) and CD34(+)/CD133(+) cell numbers compared with MA(-) patients (P < 0.006). In in vitro functional assays, MA(+) patients had a significantly lower number of colony-forming units and impaired vascular endothelial growth factor (VEGF)-A-mediated tube formation, when compared with MA(-) patients (P < 0.01).


CONCLUSIONS
In type 1 diabetic patients with microalbuminuria, a marker of microvascular injury and a risk factor for CVD, circulating vascular progenitor cell number is reduced and function is impaired.
Dessapt
Cecile
C
Cardiovascular Division, King's College, London, UK.
Karalliedde
Janaka
J
Hernandez-Fuentes
Maria
M
Prieto Martin
Paz
P
Maltese
Giuseppe
G
Dattani
Nikesh
N
Atkar
Ravinder
R
Viberti
Giancarlo
G
Gnudi
Luigi
L
D000071916:AC133 Antigen; D000419:Albuminuria / Q000473:pathology* / Q000503:physiopathology; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology*; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides