Broad spectrum drug screening using electron-ionization gas chromatography-mass spectrometry (EI-GCMS).
A liquid-liquid extraction (LLE) of drugs and internal standard (promazine) is performed by mixing urine at basic pH with 1-chlorobutane. There are no hydrolysis or derivatization steps. After centrifugation the organic (upper) layer is transferred to another tube and evaporated. The dried extract is reconstituted with ethyl acetate and 1 microL is injected onto the GCMS. Drugs are volatilized in the GC inlet and separated on a capillary column. In the EI source drugs become positively charged and fragment. Mass analysis of ionized fragments occurs with a single quadrupole. The resulting full scan mass spectra are automatically searched against three libraries.
Stone
Judy
J
Clinical Laboratories, San Francisco General Hospital, San Francisco, CA, USA.
D000085:Acetates / Q000737:chemistry; D000465:Algorithms; D002073:Butanes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000652:urine*; D011395:Promazine / Q000737:chemistry; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000085:Acetates; D002073:Butanes; D004364:Pharmaceutical Preparations; C007650:ethyl acetate; D011395:Promazine; C006854:butyl chloride