Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection.
A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 94 degrees C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity (R(2)>0.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps, which provides another view for quality control of Cordyceps sinensis.
Wang
Shuang
S
Institute of Chinese Medical Sciences, University of Macau, Macao SAR, PR China.
Yang
Feng-Qing
FQ
Feng
Kun
K
Li
De-Qiang
DQ
Zhao
Jing
J
Li
Shao-Ping
SP
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D008027:Light; D009705:Nucleosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D012542:Scattering, Radiation
D002241:Carbohydrates; D004365:Drugs, Chinese Herbal; D005229:Fatty Acids, Monounsaturated; D009705:Nucleosides; C001996:thermozymocidin