title: A validated assay for the quantitative analysis of tranexamic acid in human serum by liquid chromatography coupled with electrospray ionization mass spectrometry.
abstract: BACKGROUND
  Tranexamic acid is a synthetic lysine analog used for management of bleeding disorders. The objective of this study was first to develop a method for measurement of tranexamic acid in human serum using liquid chromatography coupled to ion-trap mass spectrometry (LC-MS/MS), and then to validate it throughout a wide range of concentrations allowing quantification in patients receiving tranexamic acid infusion during surgery.
  
  
  METHODS
  Serum samples (100 microL) were subjected to protein precipitation with perchloric acid, and after pH adjustment, tranexamic acid and internal standard were separated on a C(18) column and isocratically eluted using a mobile phase constituted of formate buffer/acetonitrile (95:5, v/v). Tranexamic acid was ionized by electrospray in positive mode. Parent [M+H](+) ions were m/z 158.0 for tranexamic acid and m/z 144.0 for IS. The most intense product ion of tranexamic acid (m/z 122.7) and IS (m/z 126.0) were used for quantification.
  
  
  RESULTS
  The assay was accurate and precise over the range of 1.0 (lower limit of quantification) to 200.0 microg/mL (upper limit of quantification), and has been successfully applied to study the clinical pharmacokinetics in two volunteers undergoing cardiac surgery.
  
  
  CONCLUSIONS
  A reliable method for quantification of tranexamic acid for analysis in clinical studies was obtained.
authors.affiliation: AP-HP, Hôpital Raymond Poincaré, Laboratory of Pharmacology-Toxicology, Garches, France. s.grassindelyle@gmail.com
authors.forename: Stanislas
authors.initials: S
authors.lastname: Grassin Delyle
authors.forename: Emuri
authors.initials: E
authors.lastname: Abe
authors.forename: Anne
authors.initials: A
authors.lastname: Batisse
authors.forename: Benjamin
authors.initials: B
authors.lastname: Tremey
authors.forename: Marc
authors.initials: M
authors.lastname: Fischler
authors.forename: Philippe
authors.initials: P
authors.lastname: Devillier
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Alvarez
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014148:Tranexamic Acid / Q000097:blood* / Q000737:chemistry
chemical_list: D014148:Tranexamic Acid