Triterpene glycosides with in vitro anti-inflammatory activity from Cyclamen repandum tubers.
A new triterpene glycoside, repandoside (1) was isolated together with six known saponins (2-7) from the methanol extract of Cyclamen repandum tubers. The isolated saponins were characterized by high resolution mass spectrometry and both 1D and 2D NMR experiments. The in vitro effect of saponins on LPS-induced IL-8 and TNF-alpha mRNA level (by quantitative RT-PCR) and protein release (by ELISA) was evaluated in human THP-1 macrophages. We found that compounds 1 (repandoside), 2 (deglucocyclamin) and 4 (anagalloside B) at 100 microM inhibited the LPS-induced IL-8 and TNF-alpha expressions.
Dall'Acqua
Stefano
S
Department of Pharmaceutical Sciences, University of Padova, Italy. stefano.dallacqua@unipd.it
Castagliuolo
Ignazio
I
Brun
Paola
P
Ditadi
Federica
F
Palù
Giorgio
G
Innocenti
Gabbriella
G
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D031824:Cyclamen / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014315:Triterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D006027:Glycosides; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012333:RNA, Messenger; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; C550231:repandoside