title: Human RECQL5beta stimulates flap endonuclease 1.
abstract: Human RECQL5 is a member of the RecQ helicase family which is implicated in genome maintenance. Five human members of the family have been identified; three of them, BLM, WRN and RECQL4 are associated with elevated cancer risk. RECQL1 and RECQL5 have not been linked to any human disorder yet; cells devoid of RECQL1 and RECQL5 display increased chromosomal instability. Here, we report the physical and functional interaction of the large isomer of RECQL5, RECQL5beta, with the human flap endonuclease 1, FEN1, which plays a critical role in DNA replication, recombination and repair. RECQL5beta dramatically stimulates the rate of FEN1 cleavage of flap DNA substrates. Moreover, we show that RECQL5beta and FEN1 interact physically and co-localize in the nucleus in response to DNA damage. Our findings, together with the previous literature on WRN, BLM and RECQL4's stimulation of FEN1, suggests that the ability of RecQ helicases to stimulate FEN1 may be a general feature of this class of enzymes. This could indicate a common role for the RecQ helicases in the processing of oxidative DNA damage.
authors.affiliation: National Institute on Aging, National Institutes of Health, 251 Bayview Blvd, Baltimore, MD 21224, USA.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Speina
authors.forename: Lale
authors.initials: L
authors.lastname: Dawut
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hedayati
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Wang
authors.forename: Alfred
authors.initials: A
authors.lastname: May
authors.forename: Sybille
authors.initials: S
authors.lastname: Schwendener
authors.forename: Pavel
authors.initials: P
authors.lastname: Janscak
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Croteau
authors.forename: Vilhelm A
authors.initials: VA
authors.lastname: Bohr
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053837:DNA Cleavage; D004277:DNA, Single-Stranded / Q000378:metabolism; D045585:Flap Endonucleases / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D053484:RecQ Helicases / Q000032:analysis / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; C506732:RECQL5 protein, human; D004247:DNA; D045585:Flap Endonucleases; D053484:RecQ Helicases