title: Methylation-sensitive single-molecule analysis of chromatin structure.
abstract: Methylation-sensitive single-molecule analysis of chromatin structure is a high-resolution method for studying nucleosome positioning. As described in this unit, this method allows for the analysis of the chromatin structure of unmethylated CpG islands or in vitro-remodeled nucleosomes by treatment with the CpG-specific DNA methyltransferase SssI (M.SssI), followed by bisulfite sequencing of individual progeny DNA molecules. Unlike nuclease-based approaches, this method allows each molecule to be viewed as an individual entity instead of an average population.
authors.affiliation: Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, USA.
authors.forename: Tina B
authors.initials: TB
authors.lastname: Miranda
authors.forename: Theresa K
authors.initials: TK
authors.lastname: Kelly
authors.forename: Karim
authors.initials: K
authors.lastname: Bouazoune
authors.forename: Peter A
authors.initials: PA
authors.lastname: Jones
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002843:Chromatin / Q000737:chemistry*; D018899:CpG Islands; D019175:DNA Methylation; D005821:Genetic Techniques; D006801:Humans
chemical_list: D002843:Chromatin