title: Tricarboxylic acid cycle-dependent synthesis of Staphylococcus aureus Type 5 and 8 capsular polysaccharides.
abstract: Staphylococcus aureus capsule synthesis requires the precursor N-acetyl-glucosamine; however, capsule is synthesized during post-exponential growth when the availability of N-acetyl-glucosamine is limited. Capsule biosynthesis also requires aerobic respiration, leading us to hypothesize that capsule synthesis requires tricarboxylic acid cycle intermediates. Consistent with this hypothesis, S. aureus tricarboxylic acid cycle mutants fail to make capsule.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska, 155 VBS, Fair St. and East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Marat R
authors.initials: MR
authors.lastname: Sadykov
authors.forename: Theodoric A
authors.initials: TA
authors.lastname: Mattes
authors.forename: Thanh T
authors.initials: TT
authors.lastname: Luong
authors.forename: Yefei
authors.initials: Y
authors.lastname: Zhu
authors.forename: Shandra R
authors.initials: SR
authors.lastname: Day
authors.forename: Costi D
authors.initials: CD
authors.lastname: Sifri
authors.forename: Chia Y
authors.initials: CY
authors.lastname: Lee
authors.forename: Greg A
authors.initials: GA
authors.lastname: Somerville
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D002952:Citric Acid Cycle; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D000117:Acetylglucosamine