title: The effect of dentine-bonding agents on substance P release in human dental pulp.
abstract: OBJECTIVE
  To quantify the effect of dentine-bonding agents on Substance P (SP) release in healthy human dental pulp tissue.
  
  
  METHODS
  Forty pulp samples were obtained from healthy pre-molars where extraction was indicated for orthodontic reasons. In thirty of these pre-molars, a standardized Class V cavity preparation was performed, and teeth were divided equally into three groups: (i) Unetched-cavity control group: Class V cavities only; (ii) Experimental Group I: 'One-step' self-etch bonding agent was placed in the cavity; and (iii) Experimental Group II: 'Two-step' total-etch bonding agent was placed in the cavity. The remaining ten healthy pre-molars where extracted without treatment and served as an intact-teeth control group. SP was measured by radioimmunoassay.
  
  
  RESULTS
  Greater SP release was found in the 'one-step' bonding agent group, followed by the 'two-step' bonding agent group and the unetched-cavity control group. The lower SP values were for the intact-teeth control group. anova showed statistically significant differences between groups (P = 0.0001). Tukey HSD post hoc tests showed statistically significant differences in SP release between the intact-teeth control group and the three other groups (P < 0.01) and between the unetched-cavity control group and the 'one-step' bonding agent group (P < 0.05). No significant difference was found between the 'two-step' bonding agent and the unetched-cavity control group.
  
  
  CONCLUSIONS
  Dentine-bonding agents placed over Class V cavity preparations increased SP release. One-step dentine-bonding agents increased SP release most.
authors.affiliation: Endodontic Department, School of Dentistry, Pontificia Universidad Javeriana, Bogota, Colombia. javiercaviedes@cable.net.co
authors.forename: J
authors.initials: J
authors.lastname: Caviedes-Bucheli
authors.forename: J A
authors.initials: JA
authors.lastname: Correa-Ortiz
authors.forename: A C
authors.initials: AC
authors.lastname: Ballestero
authors.forename: M
authors.initials: M
authors.lastname: Jimenez
authors.forename: A
authors.initials: A
authors.lastname: Munoz
authors.forename: C
authors.initials: C
authors.lastname: Rivero
authors.forename: N
authors.initials: N
authors.lastname: Lombana
authors.forename: H R
authors.initials: HR
authors.lastname: Munoz
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D003737:Dental Cavity Preparation / Q000379:methods*; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism; D003793:Dental Restoration, Permanent / Q000379:methods; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D006801:Humans; D019279:Resin Cements / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013373:Substance P / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017220:Dentin-Bonding Agents; C448319:ESPE bonding system; C451600:OptiBond Solo Plus; D019279:Resin Cements; D013373:Substance P