Chemical rescue of protein tyrosine phosphatase activity.
We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.
Chen
Vincent L
VL
Amherst College, Department of Chemistry, Amherst, MA, USA.
Bishop
Anthony C
AC
D020134:Catalytic Domain; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism*; D015870:Gene Expression; D009154:Mutation; D011485:Protein Binding; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D005452:Fluoresceins; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12