L-carnitine modulates blood platelet oxidative stress.
The oxidative stress induced by acute exertion may interfere with blood platelet activation. The beneficial effect of L-carnitine (gamma-trimethylamino-beta-hydroxybutyric acid) on oxidative stress in blood platelets has not been fully investigated; however, different studies indicate that this compound modulates platelet functions. The aim of our study was to assess the effects of L-carnitine on platelet activation and oxidative/nitrative protein damage (determined by the levels of protein carbonyl groups, thiol groups, and 3-nitrotyrosine residues) in resting blood platelets or platelets treated with peroxynitrite (ONOO(-), a strong physiological oxidant) in vitro. We also investigated the effects of L-carnitine on the level of platelet glutathione and on the formation of superoxide anion radicals O2(-*), lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) in blood platelets stimulated by thrombin (a strong physiological agonist), and platelet aggregation induced by adenosine diphosphate (a strong physiological stimulator). We have observed that carnitine decreases platelet activation (measured by platelet aggregation, the generation of O2(-*), and TBARS production). Moreover, our results in vitro demonstrate that carnitine may protect against oxidation of thiol groups induced by ONOO(-). Thus, carnitine may have some protectory effects against oxidative changes induced in blood platelets.
Saluk-Juszczak
Joanna
J
Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Lodz, Poland. juszczak@biol.uni.lodz.pl
Olas
Beata
B
Wachowicz
Barbara
B
Glowacki
Rafal
R
Bald
Edward
E
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002331:Carnitine / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D015538:Nitrosation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000097:blood; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D055815:Young Adult
D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D030421:Peroxynitrous Acid; D014443:Tyrosine; D000244:Adenosine Diphosphate; D005978:Glutathione; D002331:Carnitine