Myelogenous leukemia in adult inbred MHC-defined miniature swine: a model for human myeloid leukemias.
This manuscript reports on five cases of spontaneous myelogenous leukemia, similar to human disease, occurring within highly inbred, histocompatible sublines of Massachusetts General Hospital (MGH) MHC-defined miniature swine. In cases where a neoplasm was suspected based on clinical observations, samples were obtained for complete blood count, peripheral blood smear, and flow cytometric analysis. Animals confirmed to have neoplasms were euthanized and underwent necropsy. Histological samples were obtained from abnormal tissues and suspect lesions. The phenotype of the malignancies was assessed by flow cytometric analysis of processed peripheral blood mononuclear cells and affected tissues. Five cases of spontaneous myeloid leukemia were identified in adult animals older than 30 months of age. All animals presented with symptoms of weight loss, lethargy, and marked leukocytosis. At autopsy, all animals had systemic disease involvement and presented with severe hepatosplenomegaly. Three of the five myelogenous leukemias have successfully been expanded in vitro. The clustered incidence of disease in this closed herd suggests that genetic factors may be contributing to disease development. Myelogenous leukemia cell lines established from inbred sublines of MGH MHC-defined miniature swine have the potential to be utilized as a model to evaluate therapies of human leukemia.
Duran-Struuck
Raimon
R
Transplantation Biology Research Center, Massachusetts General Hospital, United States. raimon.duran-struuck@tbrc.mgh.harvard.edu
Cho
Patricia S
PS
Teague
Alexander G S
AG
Fishman
Brian
B
Fishman
Aaron S
AS
Hanekamp
John S
JS
Moran
Shannon G
SG
Wikiel
Krzysztof J
KJ
Ferguson
Kelly K
KK
Lo
Diana P
DP
Duggan
Michael
M
Arn
J Scott
JS
Billiter
Bob
B
Horner
Ben
B
Houser
Stuart
S
Yeap
Beow Yong
BY
Westmoreland
Susan V
SV
Spitzer
Thomas R
TR
McMorrow
Isabel M
IM
Sachs
David H
DH
Bronson
Roderick T
RT
Huang
Christene A
CA
D000740:Anemia / Q000662:veterinary; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007178:Inbreeding; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D007964:Leukocytosis / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013556:Swine, Miniature
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen; D007770:L-Lactate Dehydrogenase