title: Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.
abstract: We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Lavine
authors.forename: Philipp W
authors.initials: PW
authors.lastname: Raess
authors.forename: Dawn B
authors.initials: DB
authors.lastname: Davis
authors.forename: Mary E
authors.initials: ME
authors.lastname: Rabaglia
authors.forename: Brent K
authors.initials: BK
authors.lastname: Presley
authors.forename: Mark P
authors.initials: MP
authors.lastname: Keller
authors.forename: Margery C
authors.initials: MC
authors.lastname: Beinfeld
authors.forename: Alan S
authors.initials: AS
authors.lastname: Kopin
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Newgard
authors.forename: Alan D
authors.initials: AD
authors.lastname: Attie
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017500:Adenovirus E1A Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013045:Species Specificity; D014162:Transfection
chemical_list: D017500:Adenovirus E1A Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D002766:Cholecystokinin