title: Detection of Hepatitis E Virus (HEV) in Italian pigs displaying different pathological lesions.
abstract: In this study we investigated the HEV prevalence in Italian pigs displaying different pathological lesions, possible risk factors related to the infection, and the possible relations occurring between HEV and other concomitant pig pathogens. Genetic characterization of some of the identified strains was also performed. Detection of HEV RNA was accomplished using a nested reverse-transcription polymerase chain reaction on bile samples from 137 pigs of 2-4months of age submitted for diagnostic purposes. Forty-one of the 137 examined pigs (29.9%) tested positive for HEV RNA. Animals of 80-120days of age showed a higher prevalence of HEV infection (46.9% against 20% of younger animals). No statistically significant correlations between HEV positivity and the presence of other pathological conditions detected at necropsy, or concomitant coinfections with PCV2 and/or PRRSV were detected. All identified strains belonged to genotype 3, and were similar to other HEV subtypes 3e, 3f, 3c circulating in Europe.
authors.affiliation: Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Martelli
authors.forename: S
authors.initials: S
authors.lastname: Toma
authors.forename: I
authors.initials: I
authors.lastname: Di Bartolo
authors.forename: A
authors.initials: A
authors.lastname: Caprioli
authors.forename: F M
authors.initials: FM
authors.lastname: Ruggeri
authors.forename: D
authors.initials: D
authors.lastname: Lelli
authors.forename: M
authors.initials: M
authors.lastname: Bonci
authors.forename: F
authors.initials: F
authors.lastname: Ostanello
mesh_terms: D000375:Aging; D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D016751:Hepatitis E / Q000453:epidemiology* / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007558:Italy / Q000453:epidemiology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D012307:Risk Factors; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000235:genetics / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral