Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis.
Phagocytosis of invading microbes requires dynamic rearrangement of the plasma membrane and its associated cytoskeletal actin network. The polarization of Cdc42 and Rac1 Rho GTPases to the site of plasma membrane protrusion is responsible for the remodeling of actin structures. However, the mechanism of Rho GTPase recruitment to these sites and the identities of accessory molecules involved in this process are not well understood. In this study, we uncovered several new components involved in innate immunity in Drosophila melanogaster. Our data demonstrate that Rab35 is a regulator of vesicle transport required specifically for phagocytosis. Moreover, recruitment of Cdc42 and Rac1 to the sites of filopodium and lamellipodium formation is Rab35 dependent and occurs by way of microtubule tracks. These results implicate Rab35 as the immune cell-specific regulator of vesicle transport within the actin-remodeling complex.
Shim
Jaewon
J
Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul 120-749, South Korea.
Lee
Sun-Min
SM
Lee
Myeong Sup
MS
Yoon
Joonsun
J
Kweon
Hee-Seok
HS
Kim
Young-Joon
YJ
D000199:Actins / Q000378:metabolism; D000818:Animals; D001424:Bacterial Infections / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004198:Disease Susceptibility; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000201:enzymology* / Q000382:microbiology / Q000648:ultrastructure; D006434:Hemocytes / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D016973:Pectobacterium carotovorum / Q000502:physiology; D010587:Phagocytosis; D021381:Protein Transport; D011554:Pseudopodia / Q000378:metabolism; D034622:RNA Interference; D022161:Transport Vesicles / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D000199:Actins; D029721:Drosophila Proteins; C543586:Rab35 protein, Drosophila; D020764:cdc42 GTP-Binding Protein; D020691:rab GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein; D020741:rho GTP-Binding Proteins