title: Efficient production of 2-oxobutyrate from 2-hydroxybutyrate by using whole cells of Pseudomonas stutzeri strain SDM.
abstract: 2-Oxobutyrate is an important intermediate in the chemical, drug, and food industries. Whole cells of Pseudomonas stutzeri SDM, containing NAD-independent lactate dehydrogenases, effectively converted 2-hydroxybutyrate into 2-oxobutyrate. Under optimal conditions, the biocatalytic process produced 2-oxobutyrate at a high concentration (44.4 g liter(-1)) and a high yield (91.5%).
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Chuanjuan
authors.initials: C
authors.lastname: Lv
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Chunhui
authors.initials: C
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D001709:Biotechnology / Q000379:methods; D002087:Butyrates / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D044223:Pseudomonas stutzeri / Q000378:metabolism*
chemical_list: D002087:Butyrates; D006885:Hydroxybutyrates; C005087:alpha-ketobutyric acid; D007770:L-Lactate Dehydrogenase