title: In vitro evaluation of antioxidants of fruit extract of Momordica charantia L. on fibroblasts and keratinocytes.
abstract: The antioxidant activity of the total aqueous extract (TAE) and total phenolic extract (TPE) of Momordica charantia fruits was assayed by radical-scavenging methods and cytoprotective effects on hydrogen peroxide (H(2)O(2))- and hypoxanthin-xanthin oxidase (HX-XO)-induced damage to rat cardiac fibroblasts (RCFs), NIH 3T3, and keratinocyte (A431). Cell viability was monitored by a 3-[4,5-dimethyltriazol-2-yl]-2,5-diphenyltretrazolium (MTT) assay. For fibroblasts, TPE at 200 and 300 microg/mL showed maximum and consistent cytoprotection against oxidants. The extract at 50 microg/mL also had significant and slightly protective effects on fibroblasts against H(2)O(2)- and HX-XO-induced damage, respectively. RCF was more tolerant toward the damage. For keratinocytes, a dose-dependent relationship of oxidant toxicity was only seen with H(2)O(2) but the protective action of the extract correlated with oxidant dosage. At 200 and 300 microg/mL TPE, cytoprotection was dose-dependent against oxidants. Extracts had no effect on HX-XO toxicity at 50 microg/mL. Pretreatment with both the extracts did not show any cytoprotection.
authors.affiliation: Bio-Products Laboratory, Biomaterial Division, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600 020, Tamil Nadu, India.
authors.forename: Ramadhar
authors.initials: R
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Balaji
authors.forename: R
authors.initials: R
authors.lastname: Sripriya
authors.forename: N
authors.initials: N
authors.lastname: Nithya
authors.forename: T S
authors.initials: TS
authors.lastname: Uma
authors.forename: P K
authors.initials: PK
authors.lastname: Sehgal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D005638:Fruit / Q000737:chemistry; D015603:Keratinocytes / Q000187:drug effects*; D029777:Momordica charantia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D000975:Antioxidants; D010936:Plant Extracts