Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications.
2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.
Huang
Ying
Y
Department of Chemistry, Texas A&M University, College Station, TX 77843, United States.
Wan
Wei
W
Russell
William K
WK
Pai
Pei-Jing
PJ
Wang
Zhiyong
Z
Russell
David H
DH
Liu
Wenshe
W
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007651:Keto Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*
D000596:Amino Acids; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D007651:Keto Acids