[Distribution study of two constituents in rat after oral administration of Smilax china extract].
OBJECTIVE
To investigate the distribution in rat after oral administration of Smilax china extract.


METHODS
Oxyresveratrol and resveratrol were quantitatively determined by HPLC/DAD method in rat tissues including heart, liver, spleen, lung and kidney after oral administration of S. china extract. The separation was performed on a Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water using linear gradient elution and UV detection at wavelength of 320 nm. The flow rate was 1.0 mL x min(-1) and the column was kept at 40 degrees C.


RESULTS
The HPLC/DAD method for the simultaneous determination of oxyresveratrol and resveratrol in rat tissues was developed.


CONCLUSIONS
The method has been successfully applied for the distribution study of two active constituents in rat through oral administration of S. china extract.
Huang
Huilian
H
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. huilianh@163.com
Chen
Guangtong
G
Lu
Zhiqiang
Z
Zhang
Jinqiang
J
Guo
Dean
D
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D000077185:Resveratrol