Sensitive and rapid high-performance liquid chromatography tandem mass spectrometry method for estimation of fulvestrant in rabbit plasma.
A rapid and sensitive high-performance liquid chromatography and electrospray tandem mass spectrometry method was developed and validated for estimation of fulvestrant in rabbit plasma using liquid-liquid extraction. The separation and quantification of fulvestrant were achieved by reverse-phase chromatography on a Sunfire C18 column (50 x 2.1. i.d., 3.5 microm) with isocratic elution at a flow rate of 300 microL/min using norethistrone as an internal standard from 500 microL plasma sample. The method was validated over the concentration range from 0.092 to 16.937 ng/mL with a lower limit of detection of 0.023 ng/mL. The intra-day and inter-day accuracy and precision were within 10%. The recovery was 85 and 90% for fulvestrant and norethistrone respectively. The chromatographic run time was only 2.5 min.
Balaram
Varanasi Murali
VM
Department of Pharmaceutical Analysis, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad-34, India.
Parmar
Dharmesh
D
Teja
Bulusu B
BB
Rathnam
Shivprakash
S
Rao
Jangala Venkateswara
JV
Dasandi
Bhavesh
B
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D000077267:Fulvestrant; D011817:Rabbits; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000077267:Fulvestrant; D004958:Estradiol