Investigation of doublecortin and calcium/calmodulin-dependent protein kinase-like-1-expressing cells in the mouse stomach.
OBJECTIVE
For lack of a definite stem cell marker, there is limited knowledge of the precise location and fate of stem cells after injury. Doublecortin and calcium/calmodulin-dependent protein kinase-like-1 (DCAMKL-1) is a putative intestinal and colon stem cell marker. Our aim was to identify DCAMKL-1-expressing cells in the gastric epithelium and to analyze the fate of DCAMKL-1-expressing cells during gastric mucosal injury and repair.


METHODS
Acidified ethanol was administered to wild-type mice. DCAMKL-1 expression were detected by immunohistochemistry and western blotting.


RESULTS
There were some DCAMKL-1-expressing cells in normal mouse stomachs. All the cells were located in the gastric isthmus region. All DCAMKL-1-expressing cells were double stained with Dolichos biflorus lectin-expressing parietal cells and Musashi-1-expressing cells. The DCAMKL-1 antigen expression decreased 12 h after injury and gradually increased to normal 4 d after injury.


CONCLUSIONS
Using DCAMKL-1 as a marker for stomach stem cells, we could describe the expression pattern of stomach stem cells during mucosal injury.
Zhang
Yan
Y
Department of Gastroenterology, Sichuan University, Sichuan, China. fengyixx@sohu.com
Huang
Xinlan
X
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D000089742:Doublecortin-Like Kinases; D005753:Gastric Mucosa / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D010295:Parietal Cells, Gastric / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism
D015415:Biomarkers; D000089742:Doublecortin-Like Kinases; C485525:Dclk1 protein, mouse; D017346:Protein Serine-Threonine Kinases