Ultrathroughput multiple reaction monitoring mass spectrometry.
A novel transformation of the multiplexing potential to the throughput potential of multiple reaction monitoring mass spectrometry is presented for targeted quantitation of proteins. Herein, this method is termed as ultrathroughput multiple reaction monitoring (UMRM) mass spectrometry. It integrates the use of stable isotope dilution-multiple reaction monitoring mass spectrometry and peptide derivatization with inexpensive and commercial chemicals. One-experiment quantitation of a common signature peptide in 25 different samples demonstrates proof-of-concept for the unprecedented throughput potential of the UMRM technology.
Yao
Xudong
X
Bajrami
Bekim
B
Shi
Yu
Y
D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D010455:Peptides; D011506:Proteins