Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P).
Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generation of lipid mediators of inflammation. We describe here the generation of specific antibodies against PLB-P, the tissue localizations of PLB-P and the establishment of an accurate, specific, and reproducible radioimmunoassay (RIA). A survey of normal and malignant tissues showed strong immunostaining of PLB-P in neuronal and myeloid cells and in adrenal glands. Elevated levels were found in sera of patients with influenza A infection i.e. >1 microg/L and in gut fluids of patients with inflammatory bowel disease i.e. >20 microg/L. The levels correlated to markers of neutrophil activation, suggesting a neutrophil origin of PLB-P in these conditions. The antibodies and the assay will be useful in the future basic and clinical investigations of PLB-P.
Xu
Shengyuan
S
Dept. of Medical Sciences, Clinical Chemistry, Uppsala University, SE-751 85, Uppsala, Sweden. shengyuan.xu@medsci.uu.se
Cai
Linjun
L
Zhao
Linshu
L
Douhan-Håkansson
Lena
L
Kristjánsson
Gudjon
G
Pauksen
Karlis
K
Venge
Per
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000302:isolation & purification; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D004792:Enzyme Precursors / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000201:enzymology; D009980:Influenza A virus / Q000472:pathogenicity; D007251:Influenza, Human / Q000201:enzymology / Q000821:virology; D008245:Lysophospholipase / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D009369:Neoplasms / Q000201:enzymology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000201:enzymology*; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D055815:Young Adult
D000906:Antibodies; D015415:Biomarkers; D004792:Enzyme Precursors; D008245:Lysophospholipase