[Differential expression of proteins in peripheral blood mononuclear cells from patients with pulmonary tuberculosis.].
OBJECTIVE
To screen the differential expressed proteins of peripheral blood mononuclear cells (PBMC) in patients with pulmonary tuberculosis by comparative proteomics.


METHODS
PBMCs from 10 patients with tuberculosis and 10 healthy volunteers were collected. The total proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). The sites of differential expression were analyzed using ImageMaster 2D Elite 5.0 image analysis software, and the peptide mass finger-printing (PMF) identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The biological information of these proteins was searched in the database and confirmed by Western blot.


RESULTS
Well reproducible 2-DE maps of PBMC from the patients with tuberculosis and normal controls were established. Forteen differential protein spots were found. Twelve proteins were analyzed and identified. Pleckstrin and Ras suppressor protein1 were decreased in patients with tuberculosis while the other 10 proteins were increased. The decreased expression of Pleckstrin in tuberculosis was confirmed by Western blot.


CONCLUSIONS
Analysis of differential protein expression in PBMC from patients with pulmonary tuberculosis by comparative proteomics identified 12 proteins. The results suggest that Pleckstrin and Coronin-1A may have an important role in the pathogenesis of pulmonary tuberculosis through regulating the phagocytosis of mononuclear macrophages.
Chen
Xiao-Bo
XB
Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Liao
Dong-Jiang
DJ
Li
Shi-Yue
SY
He
Ying
Y
Chi
Feng
F
D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014397:Tuberculosis, Pulmonary / Q000378:metabolism
D020543:Proteome