Quantitation of opioids in blood and urine using gas chromatography-mass spectrometry (GC-MS).
The opioid and 6-acetylmorphine assays utilize gas chromatography-mass spectrometry (GC-MS) for the analysis of morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted by mixed-mode solid phase extraction. The morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine extracts are derivatized with N-methyl-bis(trifluoroacetamide) (MBTFA) producing trifluoroacetyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.
Goldberger
Bruce A
BA
Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Chronister
Chris W
CW
Merves
Michele L
ML
D000701:Analgesics, Opioid / Q000097:blood* / Q000652:urine*; D003061:Codeine / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006853:Hydrocodone / Q000097:blood / Q000652:urine; D004091:Hydromorphone / Q000097:blood / Q000652:urine; D009020:Morphine / Q000097:blood / Q000652:urine; D009022:Morphine Derivatives / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
D000701:Analgesics, Opioid; D009022:Morphine Derivatives; D006853:Hydrocodone; D009020:Morphine; C026979:6-O-monoacetylmorphine; D004091:Hydromorphone; D003061:Codeine