F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
Arutyunov
Denis
D
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
Arenson
Barbara
B
Manchak
Jan
J
Frost
Laura S
LS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D003227:Conjugation, Genetic / Q000502:physiology*; D017124:Conserved Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D005144:F Factor; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009687:Nuclear Proteins; C500309:TraF protein, E coli; C065598:TraH protein, Bacteria