title: Effects of dietary folic acid on the expression of myelin basic protein in the brain and spinal cord of pregnant and lactating rats.
abstract: OBJECTIVE
  This study investigated the effects of dietary folic acid on the expression of myelin basic protein (MBP) in the maternal brain and spinal cord during pregnancy and lactation.
  
  
  METHODS
  Female Sprague-Dawley rats were fed either a folic-acid-supplemented diet (FS, 8 mg/kg diet) or a folic-acid-deficient diet (FD, 0 mg/kg diet) from 2 weeks prior to mating until the end of lactation. The expressions of MBP were analyzed using Western blot analysis and immunohistochemistry, and myelin oligodendrocyte glycoprotein (MOG), and neuronal nuclear antigen by immunohistochemistry.
  
  
  RESULTS
  The cerebrocortical expression of MBP was 87% higher at day 20 of pregnancy than before pregnancy in FS animals (p < 0.05) but did not change significantly in FD animals. No significant change was observed in the hippocampus or spinal cord in either dietary treatment group. The cerebrocortical MOG and NeuN expressions were significantly lower in FD animals than in FS animals before pregnancy and increased at day 20 of pregnancy but did not differ with the dietary folic acid level.
  
  
  CONCLUSIONS
  Folic acid deficiency did not increase the expression level of MBP in the cerebral cortex during pregnancy, suggesting that folate intake during pregnancy plays an important role in the maintenance of myelin.
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Yo A
authors.initials: YA
authors.lastname: Lee
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Oh
authors.forename: Namsoo
authors.initials: N
authors.lastname: Chang
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004032:Diet / Q000379:methods; D019587:Dietary Supplements; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D005494:Folic Acid Deficiency / Q000378:metabolism; D007774:Lactation / Q000187:drug effects / Q000378:metabolism*; D023421:Models, Animal; D004676:Myelin Basic Protein / Q000187:drug effects / Q000378:metabolism*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000187:drug effects / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein; D011247:Pregnancy / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: D034961:Antigens, Nuclear; C571328:Mog protein, rat; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D014803:Vitamin B Complex; D005492:Folic Acid