[The establishment of method for identifying China vaccine strains and wild strains of measles virus].
OBJECTIVE
To establish a simple and quick method for identifying China vaccine strains and wild strains of Measles Virus.


METHODS
To search the enzyme site in Hemagglutinin gene of measles virus for different domestic vaccine strains and wild strains of measles virus, and design the RT-PCR primer within the range covering the enzyme site, and then to confirm the specificity and sensibility of the RT-PCR method, and then identify the RT-PCR product by RFLP.


RESULTS
The one-step RT-PCR method is sensitive, the measles virus of 4.64 TCID50 can be detected at least. No positive bands can be found in the non-measles virus strains, it means that the RT-PCR method has good specificity, the PCR products of Chian measles vaccine strains of Shang-191 and Chang-47 were all cut into two fragments (287 bp and 151 bp) by Afi II, but two measles wild virus strains can't be cut by Afl II.


CONCLUSIONS
The RT-PCR-RFLP method which we established is a rapid and simple method for identifying China vaccine strain and wild strain.
Zhou
Jian-Hui
JH
Center for Disease Control and Prevention of Jilin Province, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Wang
Shuang
S
Chen
Chao
C
D001483:Base Sequence; D002681:China; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008457:Measles / Q000821:virology*; D008458:Measles Vaccine / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008459:Measles virus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment
D006389:Hemagglutinins, Viral; D008458:Measles Vaccine