title: Chromosome analysis of the differential radiosensitivity of an Epstein-Barr virus (EBV)-transformed B cell line and B and T lymphocytes from the same blood donor.
abstract: OBJECTIVE
  To date, simultaneously performed investigations on the differential radiosensitivity of an Epstein-Barr virus (EBV)-transformed B cell line as well as B and T lymphocytes of human peripheral blood are not available. Thus the aim of the present study was to fill this gap by directly comparing the corresponding dose-response relationships of dicentrics obtained in blood samples from the same donor.
  
  
  METHODS
  Cell samples of whole blood or low passage cells of an EBV-transformed B cell line were irradiated by 120 kV X-rays in chambers tightly embedded in a polymethylmethacrylate phantom. Chromosome analysis was performed in phytohemagglutinin-stimulated T lymphocytes, in pokeweed mitogen-stimulated B lymphocytes and in the EBV-transformed B cell line.
  
  
  RESULTS
  Based on dose-response relationships of dicentrics, different radiosensitivity values relative to T lymphocytes were found from 1.53-1.46 for the EBV-transformed cell line, from 0.76-0.80 for resting B lymphocytes and from 2.36-2.20 for cycling B lymphocytes within the dose range from 0.25-4 Gy.
  
  
  CONCLUSIONS
  Owing to these different radiosensitivity values, care has to be taken when dose-response relationships of dicentrics determined in B cell lines are used in biological dosimetry to estimate any dose levels for radiation protection purposes.
authors.affiliation: Institute for Cell Biology, Center for Integrated Protein Science Munich, University of Munich, Germany. ernst.schmid@lrz.uni-muenchen.de
authors.forename: Ernst
authors.initials: E
authors.lastname: Schmid
authors.forename: Hartmut
authors.initials: H
authors.lastname: Roos
authors.forename: Wiebke
authors.initials: W
authors.lastname: Sauter
authors.forename: Astrid
authors.initials: A
authors.lastname: Rickinger
authors.forename: Irene
authors.initials: I
authors.lastname: Jaehnert
authors.forename: Friederike
authors.initials: F
authors.lastname: Eckardt-Schupp
mesh_terms: D001402:B-Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D001782:Blood Donors; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D004854:Herpesvirus 4, Human; D006801:Humans; D008297:Male; D011836:Radiation Tolerance; D013601:T-Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure