Minocycline attenuates experimental autoimmune encephalomyelitis in rats by reducing T cell infiltration into the spinal cord.
We investigated the anti-inflammatory effects of minocycline in EAE, an animal model of MS. Minocycline, administered for two weeks after the clinical onset, significantly decreased the cumulative and mean clinical scores of EAE. This was associated with the reduction of both CD4(+) and CD8(+) T cell numbers in the spinal cord and the downregulation of LFA-1 on T cells without affecting the cytokine production profile. The predominant cytokine produced by T cells in the spleen was IFN-gamma whereas in the CNS it was IL-17. Our results indicate that minocycline regulates T cell infiltration into the CNS without modifying the dominant cytokine production.
Nikodemova
Maria
M
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53705, USA.
Lee
Jangeun
J
Fabry
Zsuzsanna
Z
Duncan
Ian D
ID
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008911:Minocycline / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D015703:Antigens, CD; D016207:Cytokines; D016169:Lymphocyte Function-Associated Antigen-1; D003208:Concanavalin A; D008911:Minocycline