title: Vaccination with TCL plus MHSP65 induces anti-lung cancer immunity in mice.
abstract: To develop effective anti-lung cancer vaccines, we directly mixed mycobacterial heat shock protein 65 (MHSP65) and tumor cell lysate (TCL) from Lewis lung cancer cells in vitro and tested its efficacy on stimulating anti-tumor immunity. Our results showed that MHSP65-TCL immunization significantly inhibited the growth of lung cancer in mice and prolonged the survival of lung cancer bearing mice. In vivo and in vitro data suggest that MHSP65-TCL could induce specific CTL responses and non-specific immunity, both of which could contribute to the tumor inhibition. Thus, this report provides an easy approach to prepare an efficient TCL based tumor vaccine.
authors.affiliation: Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Dong
authors.forename: Luguo
authors.initials: L
authors.lastname: Sun
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.forename: Peiyin
authors.initials: P
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Hu
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.forename: Shucheng
authors.initials: S
authors.lastname: Hua
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology; D019496:Cancer Vaccines; D018827:Carcinoma, Lewis Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002457:Cell Extracts / Q000008:administration & dosage / Q000276:immunology; D048708:Cell Growth Processes / Q000187:drug effects / Q000276:immunology; D018834:Chaperonin 60 / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D047368:Tumor Burden / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D019496:Cancer Vaccines; D002457:Cell Extracts; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium