Determination of methylene blue residues in aquatic products by liquid chromatography-tandem mass spectrometry.
A method for the determination and confirmation of methylene blue (MB) in aquatic products was developed. Residues of MB were extracted from homogenized tissues with acetonitrile/sodium acetate buffer solution, and simply cleaned up with dichloromethane liquid/liquid extraction. After concentration and dissolution, the sample solutions were cleaned up by the neutral alumina and weak cation-exchange solid phase extraction (SPE) cartridge, prior to LC-MS/MS analysis. MB was determined at 1.0-20 microg/kg in eel, toasted eel and shrimp, with a limit of quantification of 0.5 microg/kg. Recovery for MB was between 73.0% and 108.3%. This method is fast, exact and sensitive. It can be applied to determine MB in aquatic products.
Xu
Jin-Zhong
JZ
Food Laboratory, Animal, Plant and Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, PR China. xujz@jsciq.gov.cn
Dai
Lin
L
Wu
Bin
B
Ding
Tao
T
Zhu
Jun-Jie
JJ
Lin
Hong
H
Chen
Hui-Lan
HL
Shen
Chong-Yu
CY
Jiang
Yuan
Y
D000818:Animals; D000935:Antifungal Agents / Q000032:analysis; D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D004524:Eels; D008452:Maximum Allowable Concentration; D008751:Methylene Blue / Q000032:analysis*; D033561:Penaeidae; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000935:Antifungal Agents; D014874:Water Pollutants, Chemical; D008751:Methylene Blue