Ngaingan virus, a macropod-associated rhabdovirus, contains a second glycoprotein gene and seven novel open reading frames.
Ngaingan virus (NGAV) was isolated from a pool of biting midges that were collected in the tropics of northern Australia. Reported here is the full-length sequence of the NGAV genome, which, at over 15.7 kb, is the largest in any rhabdovirus described to date and contains 13 genes, the highest number of genes observed in any (-) ssRNA virus. Seven of these putative genes show no significant homology to known proteins. Like viruses in the genus Ephemerovirus, NGAV possesses a second glycoprotein gene (G(NS)). Phylogenetic analyses, however, place NGAV within the yet to be classified "Hart Park" group containing Wongabel and Flanders viruses, which do not contain a second glycoprotein gene. Screening of various animal sera from northern Australia has indicated that NGAV is currently circulating in macropods (wallabies, wallaroos and kangaroos), highlighting the need for further studies to determine its potential to cause disease in these species.
Gubala
Aneta
A
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; School of Chemistry and Molecular Sciences, University of Queensland, St. Lucia, Queensland, Australia; Human Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia. Electronic address: ania.gubala@dsto.defence.gov.au.
Davis
Steven
S
Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia.
Weir
Richard
R
Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
Melville
Lorna
L
Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
Cowled
Chris
C
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
Walker
Peter
P
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
Boyle
David
D
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002519:Ceratopogonidae / Q000821:virology; D002874:Chromosome Mapping; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D010802:Phylogeny; D012209:Rhabdoviridae / Q000235:genetics*; D020539:Sequence Analysis, Protein; D014759:Viral Envelope Proteins / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D006023:Glycoproteins; D019590:Nucleocapsid Proteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins