Resistance to linezolid in a porcine Clostridium perfringens strain carrying a mutation in the rplD gene encoding the ribosomal protein L4.
Coresistance to human reserve antibiotics can be selected by antibiotics used in veterinary medicine. A Clostridium perfringens strain isolated from pig manure was resistant to the reserve drug linezolid and, simultaneously, resistant against florfenicol and erythromycin. We detected a new mutation in a highly conserved region of rplD, encoding protein L4 of the 50S ribosomal subunit. This is the first genetic substantiation of linezolid resistance in the genus Clostridium.
Hölzel
Christina S
CS
Chair of Animal Hygiene, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany. Christina.Hoelzel@wzw.tum.de
Harms
Katrin S
KS
Schwaiger
Karin
K
Bauer
Johann
J
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003016:Clostridium perfringens / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D000069349:Linezolid; D008372:Manure / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000235:genetics*; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology
D000081:Acetamides; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D008372:Manure; D023303:Oxazolidinones; D012269:Ribosomal Proteins; C056368:ribosomal protein L4; D004917:Erythromycin; C035534:florfenicol; D013839:Thiamphenicol; D000069349:Linezolid