Cell-seeding techniques in vascular tissue engineering.
Previous studies have demonstrated the benefits of cell seeding in the construction of tissue-engineered vascular grafts (TEVG). However, seeding methods are diverse and no method is clearly superior in either promoting seeding efficiency or improving long-term graft function. As we head into an era during which a variety of different TEVG are under investigation in clinical trials around the world, it is important to consider the regulatory issues surrounding the translation of these technologies. In this review, we summarize important advances in the field of vascular tissue engineering, with particular attention on cell-seeding techniques for TEVG development and special emphasis placed on regulatory issues concerning the clinical translation of these various methods.
Villalona
Gustavo A
GA
Department of Surgery, Yale University School of Medicine, Yale New Haven Hospital, New Haven, Connecticut 06520-8062, USA.
Udelsman
Brooks
B
Duncan
Daniel R
DR
McGillicuddy
Edward
E
Sawh-Martinez
Rajendra F
RF
Hibino
Narutoshi
N
Painter
Christopher
C
Mirensky
Tamar
T
Erickson
Benjamin
B
Shinoka
Toshiharu
T
Breuer
Christopher K
CK
D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D017690:Cell Transplantation / Q000379:methods*; D042783:Endothelial Cells / Q000637:transplantation; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds