[Induced RAS association domain family gene 1A gene expression by arsenic trioxide in nasopharyngeal carcinoma cell].
OBJECTIVE
To investigate the effect of arsenic trioxide (As2O3) on expression of anti-oncogene RAS association domain family gene 1A(RASSF1A) in nasopharyngeal carcinoma cell line CNE-2Z.


METHODS
CNE-2Z cells were treated with various concentrations of As2O3 for different times. The IC(50) values were detected by trypan blue stain assay. Cell cycle redistribution was analyzed by flow cytometry. The final concentration 2 micromol/L, 1 micromol/L, 0.5 micromol/L of As2O3 was added to CNE-2Z cell for succedent experiments. The controls and no drugs of CNE-2Z cells were cultivated for 48 h. Methylation specific PCR was used to detect the change of methylation status of RASSF1A gene. The expression of RASSF1A gene was detected by reverse transcription PCR and Western blot at mRNA and protein level.


RESULTS
The suppression of cell proliferation by As2O3 was time and dose-dependent. After being treated with As2O3, the IC(50) values of As2O3 were (1.50 +/- 0.05), (1.09 +/- 0.13), (0.65 +/- 0.04) micromol/L at 24, 48, and 72 h, respectively. As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle. After the effect of As2O3, the methylation of RASSF1A gene became weaker by increasing the concentration of As2O3; and the expression of RASSF1A gene became stronger at mRNA and protein level. Between different concentration of As2O3 group and no drugs group, the differences had statistical significance (P < 0.05). Along with increasing the concentration of As2O3, the expression of RASSF1A gene became stronger at mRNA and protein level, the methylation of RASSF1A gene became weaker and weaker.


CONCLUSIONS
As2O3 can activate the expression of RASSF1A gene to inhibit the cell cycle progress of nasopharyngeal carcinoma cell line.
Hu
Jun-Li
JL
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of GuangDong Medical College, Zhanjiang 524023, China.
Zhang
Yue-Fei
YF
Qiu
Zhi-Dong
ZD
Liang
Hai-Hui
HH
D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000187:drug effects*; D015870:Gene Expression; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D010087:Oxides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D001152:Arsenicals; D010087:Oxides; C411762:RASSF1 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D000077237:Arsenic Trioxide