[Role of Ezh2 in the all-trans retinoic acid induced P19 neural differentiation].
OBJECTIVE
To study the role of Ezh2 in the all-trans retinoic acid RA induced P19 neural differentiation.


METHODS
The expression of Ngn1 in the RA induced P19 cells was detected at the mRNA and protein levels using real time RT-PCR and Western blot assays. The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.


RESULTS
In the RA induced P19 cells, the recruitment of Ezh2 and its methylated substrate H3K27me3 on the promoter of Ngn 1 gene elevated in the first 2 days, and then declined rapidly, followed by the initiation of neuronal differentiation.


CONCLUSIONS
Ezh2 produces a repressive histone mark H3K27me3 in the early stage of RA induced P12 cells. By avoiding the premature expression of Ngn1 gene, Ezh2 can ensure the normal differentiation of P19 cells.
Ge
Cui-ting
CT
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing 100005, China.
Zhang
Ye
Y
Shen
Yu-fei
YF
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D000071221:Enhancer of Zeste Homolog 2 Protein; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D063151:Polycomb Repressive Complex 2; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D051792:Basic Helix-Loop-Helix Proteins; D000071221:Enhancer of Zeste Homolog 2 Protein; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones; D009419:Nerve Tissue Proteins; D063151:Polycomb Repressive Complex 2; D014212:Tretinoin; C486336:Neurog1 protein, mouse; C498410:Ezh2 protein, mouse