A method for evaluation of antioxidant activity based on inhibition of free radical-induced erythrocyte hemolysis.
There are many in vitro methods for evaluating antioxidant activity. In this chapter, we describe an operationally simple cell-based assay, oxidative hemolysis inhibition assay (OxHLIA). OxHLIA is based on inhibition of free radical-induced membrane damage in erythrocytes by antioxidants. The advantage of this method is that it uses peroxyl radicals as pro-oxidants and erythrocytes as oxidizable targets so that the results obtained reflect biologically relevant radical-scavenging activity and microlocalization of antioxidants. We also present here a comparison of OxHLIA with other common methods (DPPH, ABTS(*+), and ORAC assays).
Takebayashi
Jun
J
Food Function and Labeling Program, Incorporated Administrative Agency, National Institute of Health and Nutrition, Tokyo, Japan. jtake@nih.go.jp
Chen
Jianbin
J
Tai
Akihiro
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D005609:Free Radicals / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*
D000975:Antioxidants; D005609:Free Radicals