Tissue expression characterization of chicken adipocyte fatty acid-binding protein and its expression difference between fat and lean birds in abdominal fat tissue.
Fatty acid-binding proteins are considered to be the carriers for the transportation of intracellular fatty acids and play an important role in the development of fatness traits. Adipocyte fatty acid-binding protein (A-FABP) is one of the family members. The current study was designed to analyze the tissue expression characterization of chicken A-FABP and its expression difference between the fat and lean males in abdominal fat tissue to reveal the possible relationship between the expression of A-FABP and abdominal fat tissue development and growth in chicken. First, fusion protein glutathione S-transferase/A-FABP was induced and purified, and then the antiserum containing specific polyclonal antibodies was obtained by immunizing healthy female rabbits using the purified fusion protein. Second, tissue expression characterization of A-FABP was investigated by Western blot. Finally, A-FABP expression difference in abdominal fat tissue between the fat and lean males was investigated by real-time reverse transcription-PCR and Western blot methods. The results showed that A-FABP expressed specifically in abdominal fat tissue and the mRNA expression level of A-FABP in fat males was lower than that of lean males at 2, 3, 4, 6, 7, 9, and 10 wk of age (P<0.05), and the protein expression level of fat males was lower than that of lean males at 6 and 10 wk of age (P<0.05). These results suggested that chicken A-FABP might affect abdominal fat deposition through changing its expression level, and the possible mechanism may be that a high expression level of A-FABP induced the high lipolytic rate and led to the decreased abdominal fat mass accordingly.
Shi
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H
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, PR China.
Wang
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Q
Zhang
Q
Q
Leng
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L
Li
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H
D050153:Abdominal Fat / Q000378:metabolism*; D017667:Adipocytes; D000818:Animals; D001823:Body Composition; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger