A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and function in 3T3-L1 cells.
Adipocyte dysfunction is strongly associated with the development of insulin resistance and diabetes, and regulation of adipogenesis is important in prevention of diabetes. We prepared a 100% methanol fraction of methanolic extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit methanol fraction). When applied to 3T3-L1 preadipocyte cells, KMF promoted adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) activity, and increased triglyceride (TG) content. KMF markedly increased mRNA expression of peroxisome proliferator-activated receptor gamma (PPARgamma)-the master adipogenic transcription factor-and its target genes. Moreover, KMF increased mRNA expression and protein secretion of adiponectin, whereas mRNA expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased the production of reactive oxygen species (ROS) and nuclear factor-kappaB (NFkappaB) activation. Glucose uptake was stimulated by KMF in differentiated 3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert beneficial effects against diabetes via its ability to regulate adipocyte differentiation and function.
Abe
Daigo
D
National Agricultural Research Center for Western Region, Zentsuji, Kagawa, Japan.
Saito
Takeshi
T
Kubo
Yasutaka
Y
Nakamura
Yoshimasa
Y
Sekiya
Keizo
K
D041721:3T3-L1 Cells; D029042:Actinidia / Q000737:chemistry*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D002839:Chromans / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism; D000077288:Troglitazone
D052242:Adiponectin; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D002839:Chromans; D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D045162:Thiazolidinediones; D014280:Triglycerides; D005993:Glycerolphosphate Dehydrogenase; D000077288:Troglitazone; D005947:Glucose