title: Low-temperature preservation of isolated islets is superior to conventional islet culture before islet transplantation.
abstract: BACKGROUND
  Although culturing islets before transplantation provides flexibility for evaluation of isolated islets and pretreatment of patients, it is well-known that isolated islets deteriorate rapidly in culture. In this study, we evaluated optimal temperature for culture/preservation of isolated human islets before transplantation.
  
  
  METHODS
  Isolated islets were cultured or preserved for 48 hr in the following culture/preservation conditions: preservation at 4 degrees C in University of Wisconsin solution and culture at 22 degrees C or 37 degrees C in culture medium.
  
  
  RESULTS
  Islet morphology after 4 degrees C preservation was similar to that of fresh islets, whereas islet diameter after 37 degrees C or 22 degrees C culture was smaller than that of fresh islets. Islet yield significantly decreased at higher temperatures (24% loss in 37 degrees C culture and 19% loss in 22 degrees C culture, but <5% loss in 4 degrees C preservation). Cultured/preserved islets were transplanted into diabetic nude mice. The attainability of posttransplantation normoglycemia was significantly higher in the 4 degrees C preservation group than in 22 degrees C and 37 degrees C culture groups.
  
  
  CONCLUSIONS
  Preservation of isolated islets at 4 degrees C improves the outcome of islet transplantation more efficiently than preservation at 22 degrees C or 37 degrees C. Based on these data, we have performed short-time cold storage of isolated islets instead of culturing for current clinical islet transplantation.
authors.affiliation: Baylor All Saints Medical Center, Baylor Research Institute, Fort Worth, TX 76104, USA. hirofumn@baylorhealth.edu
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Bashoo
authors.initials: B
authors.lastname: Naziruddin
authors.forename: Andrew
authors.initials: A
authors.lastname: Jackson
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimoda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikemoto
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Fujita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Chujo
authors.forename: Morihito
authors.initials: M
authors.lastname: Takita
authors.forename: Naoya
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Nicholas
authors.initials: N
authors.lastname: Onaca
authors.forename: Marlon F
authors.initials: MF
authors.lastname: Levy
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001926:Brain Death; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D003470:Culture Media; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D016381:Islets of Langerhans Transplantation / Q000379:methods / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D003470:Culture Media; D000255:Adenosine Triphosphate