A simple fluorescence quenching method for berberine determination using water-soluble CdTe quantum dots as probes.
A novel method for the determination of berberine has been developed based on quenching of the fluorescence of thioglycolic acid-capped CdTe quantum dots (TGA-CdTe QDs) by berberine in aqueous solutions. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of berberine between 2.5x10(-8) and 8.0x10(-6)mol L(-1) with a detection limit of 6.0x10(-9)mol L(-1). The method has been applied to the determination of berberine in real samples, and satisfactory results were obtained. The mechanism of the proposed reaction was also discussed.
Cao
Ming
M
Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, PR China.
Liu
Meigui
M
Cao
Chun
C
Xia
Yunsheng
Y
Bao
Linjun
L
Jin
Yingqiong
Y
Yang
Song
S
Zhu
Changqing
C
D001599:Berberine / Q000032:analysis*; D002104:Cadmium / Q000737:chemistry*; D002138:Calibration; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D013864:Thioglycolates; D002104:Cadmium; D014867:Water; D001599:Berberine; D013691:Tellurium