Hydrogen bonding progressively strengthens upon transfer of the protein urea-denatured state to water and protecting osmolytes.
Using osmolyte cosolvents, we show that hydrogen-bonding contributions can be separated from hydrophobic interactions in the denatured state ensemble (DSE). Specifically, the effects of urea and the protecting osmolytes sarcosine and TMAO are reported on the thermally unfolded DSE of Nank4-7*, a truncated notch ankyrin protein. The high thermal energy of this state in the presence and absence of 6 M urea or 1 M sarcosine solution is sufficient to allow large changes in the hydrodynamic radius (R(h)) and secondary structure accretion without populating the native state. The CD change at 228 nm is proportional to the inverse of the volume of the DSE, giving a compact species equivalent to a premolten globule in 1 M sarcosine. The same general effects portraying hierarchical folding observed in the DSE at 55 degrees C are also often seen at room temperature. Analysis of Nank4-7* DSE structural energetics at room temperature as a function of solvent provides rationale for understanding the structural and dimensional effects in terms of how modulation of the solvent alters solvent quality for the peptide backbone. Results show that while the strength of hydrophobic interactions changes little on transferring the DSE from 6 M urea to water and then to 1 M TMAO, backbone-backbone (hydrogen-bonding) interactions are greatly enhanced due to progressively poorer solvent quality for the peptide backbone. Thus, increased intrachain hydrogen bonding guides secondary structure accretion and DSE contraction as solvent quality is decreased. This process is accompanied by increasing hydrophobic contacts as chain contraction gathers hydrophobes into proximity and the declining urea-backbone free energy gradient reaches urea concentrations that are energetically insufficient to keep hydrophobes apart in the DSE.
Holthauzen
Luis Marcelo F
LM
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-1052, USA.
Rösgen
Jörg
J
Bolen
D Wayne
DW
D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008744:Methylamines / Q000737:chemistry; D009995:Osmosis; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011489:Protein Denaturation / Q000235:genetics; D055550:Protein Stability; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D051880:Receptors, Notch / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012521:Sarcosine / Q000737:chemistry; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry*; D014867:Water / Q000737:chemistry* / Q000378:metabolism
D029721:Drosophila Proteins; D008744:Methylamines; C497759:N protein, Drosophila; D010446:Peptide Fragments; D051880:Receptors, Notch; D014867:Water; D014508:Urea; C005855:trimethyloxamine; D012521:Sarcosine