title: Use of a novel assay to evaluate enzymes that produce hydrogen sulfide in Fusobacterium nucleatum.
abstract: To evaluate enzymes that produce hydrogen sulfide (H(2)S) in species of Fusobacterium nucleatum, we developed an assay based on SDS-polyacrylamide gel electrophoresis with renaturation followed by active staining. This assay provided precise insight into the enzymes that produce H(2)S in terms of their number and molecular weights.
authors.affiliation: Department of Pathogenesis and Control of Oral Disease, Iwate Medical University School of Dentistry, Morioka, Iwate, Japan. yasuoy@iwate-med.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Ito
authors.forename: Haruki
authors.initials: H
authors.lastname: Tamura
authors.forename: Kazushi
authors.initials: K
authors.lastname: Kunimatsu
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005674:Fusobacterium Infections / Q000150:complications / Q000201:enzymology* / Q000382:microbiology*; D016967:Fusobacterium nucleatum / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008970:Molecular Weight; D010510:Periodontal Diseases / Q000201:enzymology / Q000209:etiology; D013045:Species Specificity; D013194:Staining and Labeling
chemical_list: D006862:Hydrogen Sulfide