Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System.
This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.
An
S S A
SS
Department of Bionano Technology, Kyungwon University, Sungnam-si, Kyungki-do 461-701, South Korea. seong_an@gmail.com
Lim
K T
KT
Oh
H J
HJ
Lee
B S
BS
Zukic
E
E
Ju
Y R
YR
Yokoyama
T
T
Kim
S Y
SY
Welker
E
E
D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018620:PrPSc Proteins / Q000097:blood* / Q000737:chemistry; D055503:Protein Multimerization; D012608:Scrapie / Q000097:blood*
D018620:PrPSc Proteins