Characterization of chemical composition of Agaricus brasiliensis polysaccharides and its effect on myocardial SOD activity, MDA and caspase-3 level in ischemia-reperfusion rats.
Gas chromatography/mass spectrometry (GC-MS) were used to characterize the polysaccharides in Agaricus brasiliensis. GC-MS analysis showed that the A. brasiliensis polysaccharide was a typical heteropolysaccharide and mainly composed of glucose, arabinose and mannose in the molar percentages of 78.38%, 10.46% and 8.51%, respectively. The Fourier-transform infrared spectra (FT-IR) of A. brasiliensis polysaccharides revealed typical characteristics of polysaccharides. The samples had the characteristics of hydroxyl groups, C-H band and alpha-pyranoses. Ischemia-reperfusion treatment markedly decreased myocardial SOD activity and increased MDA level in rats treated with ischemia-reperfusion. Pharmacological experiment showed that administration of A. brasiliensis polysaccharide could significantly enhance myocardial SOD activity and reduce MDA level in rats treated with ischemia-reperfusion. At the same time, administration of A. brasiliensis polysaccharide could still significantly reduce (p<0.01) caspase-3 level in rats' brain. Results indicated that A. brasiliensis polysaccharide was beneficial in some cardiovascular diseases.
Zhang
Song
S
Department of Cardiovascular Diseases, Eastern District of Renji Hospital, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
He
Ben
B
Ge
Junbo
J
Zhai
Changlin
C
Liu
Xin
X
Liu
Pingang
P
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D053148:Caspase 3 / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism*
D011134:Polysaccharides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D053148:Caspase 3