title: Expression of homing receptors on IgA1 and IgA2 plasmablasts in blood reflects differential distribution of IgA1 and IgA2 in various body fluids.
abstract: Although secretory IgA is the most abundantly produced Ig isotype, the mechanisms underlying the differential distribution of IgA subclasses in various body fluids remain unclear. To explore these mechanisms, we examined the distribution of IgA subclasses, the influence of the nature and sites of encounters with antigens, and the correlation between IgA subclass distribution and homing potentials of circulating IgA plasmablasts. IgA1 predominated in serum, tears, nasal wash fluid, and saliva; the levels of IgA1 and IgA2 were comparable in vaginal wash fluid; and IgA2 predominated in intestinal lavage fluids. Seventy-one percent of circulating IgA plasmablasts secreted IgA1. The intestinal homing receptor (HR), alpha4beta7, was expressed more frequently on IgA2 than on IgA1 plasmablasts, with no differences in the expression of other HRs. IgA subclass distribution among circulating antigen-specific antibody-secreting cells (ASC) was dependent on the nature of the antigen: following vaccination with Salmonella enterica serovar Typhi, unconjugated pneumococcal polysaccharide, or Haemophilus influenzae polysaccharide-diphtheria toxoid conjugate, the proportions of specific IgA1 ASC were 74%, 47%, 56%, and 80%, respectively. HR expression depended on the route of administration: expression of HRs was different after oral than after parenteral vaccination, while no difference was seen between HR expression of antigen-specific IgA1 and IgA2 ASC induced via the same route. The key factors determining IgA subclass distribution in a given secretion are the nature of the antigens encountered at a particular site and the site-specific homing instructions given to lymphocytes at that site. These two factors are reflected as differences in the homing profiles of the total populations of circulating IgA1 and IgA2 plasmablasts.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Sari H
authors.initials: SH
authors.lastname: Pakkanen
authors.forename: Jussi M
authors.initials: JM
authors.lastname: Kantele
authors.forename: Zina
authors.initials: Z
authors.lastname: Moldoveanu
authors.forename: Spencer
authors.initials: S
authors.lastname: Hedges
authors.forename: Miikka
authors.initials: M
authors.lastname: Häkkinen
authors.forename: Jiri
authors.initials: J
authors.lastname: Mestecky
authors.forename: Anu
authors.initials: A
authors.lastname: Kantele
mesh_terms: D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology*; D001826:Body Fluids / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis / Q000276:immunology*; D055815:Young Adult
chemical_list: D007070:Immunoglobulin A; D016201:Receptors, Lymphocyte Homing