tRNA mitochondrial import in yeast: Mapping of the import determinants in the carrier protein, the precursor of mitochondrial lysyl-tRNA synthetase.
Mitochondria of many species import of nuclear DNA-encoded tRNAs. This widely spread but poorly studied phenomenon proved to be a promising tool for mitochondrial transfection. In yeast Saccharomyces cerevisiae, one cytosolic tRNAs(Lys) is partially targeted into mitochondria. Previous studies have shown that binding of this tRNA to its putative protein carrier, the precursor of mitochondrial lysyl-tRNA synthetase (preMsk1p), IIb class aminoacyl-tRNA synthetase, was a pre-requisite of import. In this work, we identify the hinge region with two adjacent helices H5 and H7 to be responsible for mitochondrial targeting of the tRNA and characterize preMsk1p versions with altered tRK1 import capacities.
Kamenski
Piotr
P
UMR No. 7156, CNRS - Université de Strasbourg, Department Molecular and Cellular Genetics, 21 rue René Descartes, 67084 Strasbourg, France.
Smirnova
Ekaterina
E
Kolesnikova
Olga
O
Krasheninnikov
Igor A
IA
Martin
Robert P
RP
Entelis
Nina
N
Tarassov
Ivan
I
D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012357:RNA, Transfer, Lys / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017384:Sequence Deletion
D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D050505:Mutant Proteins; D011498:Protein Precursors; D012357:RNA, Transfer, Lys; D029701:Saccharomyces cerevisiae Proteins; D008250:Lysine-tRNA Ligase