Polychlorinated biphenyls disrupt blood-brain barrier integrity and promote brain metastasis formation.
BACKGROUND
Polychlorinated biphenyls (PCBs) comprise a ubiquitous class of toxic substances associated with carcinogenic and tumor-promoting effects as well as neurotoxic properties in the brain. However, the effects of PCBs on the development of tumor metastases are not fully understood.


OBJECTIVE
We evaluated the hypothesis that exposure to individual PCB congeners can facilitate the development of brain metastases in immunocompetent mice via the disruption of the integrity of the blood-brain barrier (BBB).


METHODS
C57/Bl6 mice were exposed to individual PCBs by oral gavage, and 48 hr later they were injected with luciferase-labeled K1735 M2 melanoma cells into the internal carotid artery. The development of metastatic nodules was monitored by bioluminescent imaging. In addition, we evaluated the functional permeability of the BBB by measuring permeability of sodium fluorescein across the brain microvessels. Expression and colocalization of tight junction (TJ) proteins were studied by Western blotting and immunofluorescence microscopy.


RESULTS
Oral administration of coplanar PCB126, mono-ortho-substituted PCB118, and non-coplanar PCB153 (each at 150 micromol/kg body weight) differentially altered expression of the TJ proteins claudin-5, occludin, and zonula occludens-1 in brain capillaries. These alterations were associated with increased permeability of the BBB. Most importantly, exposure to individual PCB congeners enhanced the rate of formation and progression of brain metastases of luciferase-tagged melanoma cells.


CONCLUSIONS
Our results show for the first time that exposure to individual PCBs can facilitate the formation of bloodborne metastases via alterations of the integrity of the brain capillary endothelium.
Seelbach
Melissa
M
Molecular Neuroscience and Vascular Biology Laboratory, Department of Neurosurgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA.
Chen
Lei
L
Powell
Anita
A
Choi
Yean Jung
YJ
Zhang
Bei
B
Hennig
Bernhard
B
Toborek
Michal
M
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000139:chemically induced* / Q000473:pathology; D045744:Cell Line, Tumor; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D008545:Melanoma / Q000139:chemically induced* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D062793:Occludin; D010750:Phosphoproteins / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D062826:Zonula Occludens-1 Protein
D004785:Environmental Pollutants; D008565:Membrane Proteins; D062793:Occludin; C568986:Ocln protein, mouse; D010750:Phosphoproteins; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein; D011078:Polychlorinated Biphenyls