[Sequence analysis of hemin-binding peptide derived from recombinant hemagglutinin-2 of Porphyromonas gingivalis].
OBJECTIVE
To determine the sequence of the active peptide derived from recombinant hemagglutinin (rHA-2) of Porphyromonas gingivalis (Pg).


METHODS
The HA-2 gene from PgATCC33277 was cloned, expressed in Escherichia coli (Ec) BL21 (DE3), and purified. The purified recombinant protein was evaluated for its ability to bind hemin-linked agarose. The active peptide was subjected to endoproteinase-mediated sequence analysis.


RESULTS
The protein expressed in Ec BL21 (DE3) was identified as PgHA-2 by plasmid sequence analysis, Western blotting, and mass spectrometry. The recombinant protein was confirmed functional by its ability to bind hemin. The sequence of the active peptide of rHA-2 was determined to be DHYAVMISKTGTNAG.


CONCLUSIONS
The availability of sequence of the active peptide of rHA-2 provides a foundation for the development of immunoprophylactic and therapeutic agents against this human pathogen.
Yang
Qiu-bo
QB
Beijing Institute for Dental Research, Capital Medical University School of Stomatology, Beijing 100050, China. qiuboyang2003@163.com
Yu
Fei-yan
FY
Yang
Sheng-hui
SH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000235:genetics; D020539:Sequence Analysis, Protein
D001426:Bacterial Proteins; D006388:Hemagglutinins