Polyphosphate kinase affects oxidative stress response by modulating cAMP receptor protein and rpoS expression in Salmonella typhimurium.
Polyphosphate (polyP) plays diverse physiological functions in prokaryotes and eukaryotes, but most of their detailed mechanisms are still obscure. Here, we show that deletion of polyphosphate kinase (PPK), the principal enzyme responsible for synthesis of polyP, resulted in augmented expression of cAMP receptor protein (CRP) and rpoS and lowered H2O2 sensitivity in Salmonella Typhimurium ATCC14028. The binding of cAMP-CRP complex to rpoS promoter and further stimulation of its transcription were proved through electrophoretic mobility shift assay, lacZ fusion, and exogenous cAMP addition, respectively. The rpoS expression increased in cpdA (cAMP phosphodiesterase coding gene) mutant, further suggesting that cAMP-CRP upregulated rpoS expression. These results demonstrate that PPK affects oxidative stress response by modulating crp and rpoS expression in S. Typhimurium.
Cheng
Yuanyuan
Y
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Sun
Baolin
B
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000172:deficiency / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics; D002373:Cyclic AMP Receptor Protein / Q000096:biosynthesis / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000172:deficiency* / Q000235:genetics; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D015854:Up-Regulation
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D006861:Hydrogen Peroxide; C102664:hydroperoxidase II; D002374:Catalase; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D015105:3',5'-Cyclic-AMP Phosphodiesterases