Analysis of cytokine pattern in exhaled breath condensate of lung  transplant recipients with bronchiolitis obliterans syndrome.
BACKGROUND
Exhaled breath condensate (EBC) analysis is a promising method for investigating airway pathology. In this pilot study we tested the cytokine pattern of EBC of lung transplant patients with and without clinical evidence of bronchiolitis obliterans syndrome (BOS).


METHODS
Breath condensates collected from eight BOS patients and eight stable BOS-free lung transplant recipients in three consecutive visits were pooled in order to increase protein concentration and were then used for antibody microarray analysis detecting 120 cytokines simultaneously.


RESULTS
Nine cytokines exhibited more than twofold increase and four exhibited more than twofold decrease in BOS patients as compared to stable subjects.


CONCLUSIONS
We conclude that inflammatory cytokines are present in EBC of lung transplant recipients, however the potential benefit of detecting the EBC proteome warrants further studies.
Antus
Balazs
B
Department of Pathophysiology, National Koranyi Institute for TB and Pulmonology, Budapest, Hungary. antusb@hotmail.com
Barta
Imre
I
Czebe
Krisztina
K
Horvath
Ildiko
I
Csiszer
Eszter
E
D051722:ADAM Proteins / Q000378:metabolism; D000328:Adult; D001944:Breath Tests; D001989:Bronchiolitis Obliterans / Q000378:metabolism* / Q000601:surgery*; D016207:Cytokines / Q000378:metabolism*; D045853:Exhalation; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D016040:Lung Transplantation; D008297:Male; D010865:Pilot Projects; D014180:Transplantation; D025521:Tumor Suppressor Proteins / Q000378:metabolism
D016207:Cytokines; D051100:Glial Cell Line-Derived Neurotrophic Factor; D018793:Interleukin-13; D025521:Tumor Suppressor Proteins; D016753:Interleukin-10; D051722:ADAM Proteins; C090222:ADAM11 protein, human