title: Apolipophorin-III expression and low density lipophorin formation during embryonic development of the silkworm, Bombyx mori.
abstract: We examined the expression of apolipophorin-III (apoLp-III) during embryonic development of the silkworm Bombyx mori. ApoLp-III mRNA was first expressed 24h after oviposition, which corresponds to the time of germ band formation. The amount of apoLp-III in the eggs increased from day 2, peaked on day 4, and then gradually decreased until hatching (on day 9.5). ApoLp-III was apparently synthesized during early embryogenesis, as radioactive amino acids were incorporated into newly synthesized apoLp-III in three-day-old eggs. Moreover, radioactive apoLp-III was found only in the embryo and not in the extraembryonic tissue. KBr density gradient ultracentrifugation of egg homogenates showed that apoLp-III was associated with low-density lipophorin (LDLp). These results suggest that LDLp is required for the delivery of lipids for organogenesis during embryogenesis.
authors.affiliation: National Institute of Infectious Diseases, Tokyo, Japan. kozo@nih.go.jp
authors.forename: Kozo
authors.initials: K
authors.lastname: Tsuchida
authors.forename: Takeru
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakudoh
authors.forename: Chihiro
authors.initials: C
authors.lastname: Katagiri
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Tsurumaru
authors.forename: Naoko
authors.initials: N
authors.lastname: Takada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Fujimoto
authors.forename: Rolf
authors.initials: R
authors.lastname: Ziegler
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Iwano
authors.forename: Kunikatsu
authors.initials: K
authors.lastname: Hamano
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Yaginuma
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000378:metabolism*; D012831:Bombyx / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D019476:Insect Proteins; D008074:Lipoproteins; C042470:apolipophorin III; C032830:lipophorin