Quantitation of nicotine, its metabolites, and other related alkaloids in urine, serum, and plasma using LC-MS-MS.
We describe a method for the quantitative analysis of nicotine, cotinine, trans-3'-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS.
Yue
Bingfang
B
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
Kushnir
Mark M
MM
Urry
Francis M
FM
Rockwood
Alan L
AL
D000470:Alkaloids / Q000097:blood* / Q000652:urine*; D000691:Anabasine / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D003367:Cotinine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D006801:Humans; D009538:Nicotine / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000470:Alkaloids; C001381:hydroxycotinine; D009538:Nicotine; C005311:nornicotine; D003367:Cotinine; D000691:Anabasine