Different levels of Hsp72 in female rat myocardium in response to voluntary exercise and forced exercise.
BACKGROUND
Physical exercise induces hemodynamic stress.


OBJECTIVE
To evaluate if voluntary running and forced running induced different levels of stress protein (Hsp72) in the myocardium of female Wistar rats.


METHODS
Female rats were randomly assigned to the following groups: forced treadmill running group (FR; n= 6), voluntary running group (VR; n=6) and control group (C; n=6). VR group animals had free access to running wheels, and those from FR group underwent a running program on a treadmill (18 m/min, 60 min/day, 5 days/wk) for 8 weeks. Left ventricle (LV) and right ventricle (RV) fragments were collected at sacrifice, and the relative immunoblot contents of stress protein (Hsp72) were determined.


RESULTS
VR animals ran on average 4.87 km/wk, and FR rats ran 4.88 km/wk. Animals from VR and FR groups had less body weight gain (p<0.05) than those from C group (81.67 +/- 11.95g vs 81.17 +/- 10.18g vs 111.50 +/- 2.26g, respectively). Heart weight/body weight ratio was not significantly different (p>0.05) among VR, FR and C groups (4.54 +/- 0.79 mg/g vs 4.94 +/- 0.89 mg/g vs 4.34 +/- 0.87 mg/g, respectively). FR group animals had levels of Hsp72 (p<0.05) higher than those from VR, both in LV (287.45 +/- 35.86 % vs 135.59 +/- 5.10 %, respectively) and RV (241.31 +/- 25.83 % vs 137.91 +/- 45.20 %, respectively).


CONCLUSIONS
Voluntary running and forced running induced different levels of Hsp72 in the myocardium of female Wistar rats.
Melo
Stéphano Freitas Soares
SF
Universidade Federal de Viçosa, Viçosa, MG, Brasil.
Lunz
Wellington
W
Fontes
Elizabeth Pacheco Batista
EP
Dias
Cristina Maria Ganns Chaves
CM
Carneiro
Miguel Araujo
MA
Moura
Anselmo Gomes de
AG
Del Carlo
Ricardo Junqueira
RJ
Natali
Antonio Jose
AJ
D000818:Animals; D001835:Body Weight / Q000502:physiology; D004812:Epidemiologic Methods; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D006352:Heart Ventricles / Q000473:pathology; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012420:Running / Q000502:physiology*
D050884:HSP72 Heat-Shock Proteins