Loss-of-function mutations of retromer large subunit genes suppress the phenotype of an Arabidopsis zig mutant that lacks Qb-SNARE VTI11.
Arabidopsis thaliana zigzag (zig) is a loss-of-function mutant of Qb-SNARE VTI11, which is involved in membrane trafficking between the trans-Golgi network and the vacuole. zig-1 exhibits abnormalities in shoot gravitropism and morphology. Here, we report that loss-of-function mutants of the retromer large subunit partially suppress the zig-1 phenotype. Moreover, we demonstrate that three paralogous VPS35 genes of Arabidopsis have partially overlapping but distinct genetic functions with respect to zig-1 suppression. Tissue-specific complementation experiments using an endodermis-specific SCR promoter show that expression of VPS35B or VPS35C cannot complement the function of VPS35A. The data suggest the existence of functionally specialized paralogous VPS35 genes that nevertheless share common functions.
Hashiguchi
Yasuko
Y
Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan.
Niihama
Mitsuru
M
Takahashi
Tetsuya
T
Saito
Chieko
C
Nakano
Akihiko
A
Tasaka
Masao
M
Morita
Miyo Terao
MT
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D018522:Gravitropism; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D050766:Qb-SNARE Proteins; C120751:vesicle transport v-SNARE 11 protein, Arabidopsis