title: Mannose-binding lectin level and deficiency is not associated with inflammatory bowel diseases, disease phenotype, serology profile, and NOD2/CARD15 genotype in a large Hungarian cohort.
abstract: Mannose-binding lectin (MBL) is a major, soluble, pattern-recognition molecule and an important component of the innate host defense. The role of MBL in inflammatory bowel diseases (IBDs) is controversial. We determined the prevalence of MBL deficiency in a Hungarian IBD patients' cohort, and whether it is associated with the antimicrobial antibody formation or particular clinical manifestations. Nine hundred ninety IBD patients and 225 healthy subjects were investigated. Sera were assayed for MBL and a panel of antimicrobial antibodies (anti-OMP, anti-Saccharomyces cerevisiae antibodies, and antiglycans) by ELISA. TLR4 and NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism. Median MBL level was not significantly different between IBDs (Crohn's disease [CD]: 929; ulcerative colitis [UC]: 810 ng/ml) and the control group (1027 ng/ml), as well as the prevalence of absolute MBL deficiency (<100 ng/ml) (CD: 15.0%, UC: 18.4%, controls: 15.6%). The presence of a low MBL level (<500 ng/ml) was not associated with any of the examined serologic markers, or their combinations. In addition, there was no association with the clinical presentation, disease course, or response to treatment. TLR4 variant genotype was more common in CD patients without MBL deficiency (11% vs. 1.7%, OR: 7.29, 95% CI: 1.08-53.9, p = 0.02). We failed to confirm any association between MBL deficiency and serologic marker positivity. MBL deficiency was not predictive for clinical phenotype or disease activity in IBDs.
authors.affiliation: 2nd Department of Medicine, University of Debrecen, Debrecen, Hungary. drpappm@yahoo.com
authors.forename: Maria
authors.initials: M
authors.lastname: Papp
authors.forename: Peter Laszlo
authors.initials: PL
authors.lastname: Lakatos
authors.forename: Jolan
authors.initials: J
authors.lastname: Harsfalvi
authors.forename: Gyula
authors.initials: G
authors.lastname: Farkas
authors.forename: Karoly
authors.initials: K
authors.lastname: Palatka
authors.forename: Miklos
authors.initials: M
authors.lastname: Udvardy
authors.forename: Tamas
authors.initials: T
authors.lastname: Molnar
authors.forename: Klaudia
authors.initials: K
authors.lastname: Farkas
authors.forename: Ferenc
authors.initials: F
authors.lastname: Nagy
authors.forename: Gabor
authors.initials: G
authors.lastname: Veres
authors.forename: Laszlo
authors.initials: L
authors.lastname: Lakatos
authors.forename: Agota
authors.initials: A
authors.lastname: Kovacs
authors.forename: Tamas
authors.initials: T
authors.lastname: Dinya
authors.forename: Agnes Katalin
authors.initials: AK
authors.lastname: Kocsis
authors.forename: Janos
authors.initials: J
authors.lastname: Papp
authors.forename: Istvan
authors.initials: I
authors.lastname: Altorjay
mesh_terms: D000328:Adult; D000908:Antibodies, Fungal / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004252:DNA Mutational Analysis; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006814:Hungary; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D033681:Mitochondrial Membrane Transport Proteins / Q000276:immunology*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics; D011110:Polymorphism, Genetic; D029701:Saccharomyces cerevisiae Proteins / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D000908:Antibodies, Fungal; D015415:Biomarkers; D037601:Mannose-Binding Lectin; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D029701:Saccharomyces cerevisiae Proteins; C495345:TLR4 protein, human; C489039:TOM70 protein, S cerevisiae; D051197:Toll-Like Receptor 4