[Study on beta-amyloid peptide 1-42 induced chemokine RANTES in U251 cell].
OBJECTIVE
To study the molecular mechanisms of beta-amyloid 1-42 (Abeta1.42) induced the inflammatory response in U251 cells.


METHODS
U251 cells were treated with different concentration of Abeta1-42 (0.2-4.0 micromol/L) for 24 h, and the cell survival rate was evaluated by MTT. After treated with 2.0 micromol/L Abeta1-42 for 12, 24, 36, 48 hours, the nitric oxide content of U251 cells were detected with nitrate reductase method. The RANTES expression was tested by dual-antibody sandwich ELISA method. The expression of NF-kappaB and STAT1 were detected by the immunocytochemical staining.


RESULTS
Abeta1-42 decreased the survival rate of U251 cells in dose-dependent manner. The NO generated in 2 micromol/L Abeta1-42 treated U251 cells reached its peak at 24 h and gradually decreased thereafter. The expression of RANTES increased 4-fold (P < 0.01). The NF-kappaB P65, the STAT1 moved from the cytoplasm to the nucleus and expressed significantly were also observed in the 2 micromol/L Abeta1-42 treated U251 cells.


CONCLUSIONS
Abeta1-42 decreases the survival rate of U251 cells and induce the releasing of NO and RANTES. This indicates that Abeta1-42 induced chemokine RANTES in U251 cell may be close related to the activation of NF-kappa B and STAT1.
Hao
Jie
J
Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wang
Zhao-Dan
ZD
Yang
Yun-Xia
YX
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000378:metabolism*; D005910:Glioma / Q000473:pathology; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D050794:STAT1 Transcription Factor / Q000378:metabolism
D016229:Amyloid beta-Peptides; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D016328:NF-kappa B; D010446:Peptide Fragments; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; C075222:amyloid beta-protein (1-42); D009569:Nitric Oxide