title: Effect of date seed oil on p53 expression in normal human skin.
abstract: A critical step to escape from the carcinogenic potential of UV radiation is mediated by the protein p53. P53 activates growth arrest, allowing for DNA repair, which removes damaged cells. The concept of photoprotection involves blocking apoptosis and the prevention of oxidative damage to cellular DNA. Date seed oil (DSO) extract has been reported to be beneficial in the reduction of chemically induced oxidative stress in normal human skin. In this study, we investigated the DNA-protective qualities of DSO as measured by p53 expression in human skin biopsies, one day after exposure to ultraviolet B (UVB) radiation. P53 expression was analyzed using immunohistochemistry. The results showed an increase in p53 expression in the basal cell compartment of UVB-exposed skin as compared to the non-UVB-exposed skin. However, DSO has significant photoprotective effects by inhibition of damage caused by UVB irradiation: a significantly lower fraction of cells was p53 positive as compared to the non-DSO-treated skin. We conclude that p53 expression is a sensitive parameter for the detection of UVB-induced damage in the skin and suggest that DSO could provide an efficient complement to photoprotective measures and may contribute to reduce the DNA damage.
authors.affiliation: Unité de Recherche, Pathologies Humaines et Stress Oxydatif, Institut Supérieur de Biotechnologie de Sfax, Sfax, Tunisie. inesdammak@yahoo.fr
authors.forename: Ines
authors.initials: I
authors.lastname: Dammak
authors.forename: Sonia
authors.initials: S
authors.lastname: Boudaya
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Abdallah
authors.forename: Hamida
authors.initials: H
authors.lastname: Turki
authors.forename: Hamadi
authors.initials: H
authors.lastname: Attia
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001706:Biopsy; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D013471:Sunburn / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010938:Plant Oils; D016159:Tumor Suppressor Protein p53