title: Identification of yeast Art5 as a multicopy suppressor for the mitochondrial translocator maintenance protein Tam41.
abstract: Normal mitochondrial protein import requires multiple translocator complexes in the outer and inner mitochondrial membrane. Tam41 is a peripheral inner membrane protein that is involved in the structural maintenance of the inner membrane translocator the TIM23 complex. Here we identified an arrestin-related protein Art5 as a multicopy suppressor for the Tam41-deficient yeast mutant, which exhibited the deteriorated TIM23 complex and temperature-sensitive growth defects. Overexpression of Art5 suppressed growth defects of tam41Delta cells and partially restored the destabilized TIM23 complex structure in tam41Delta mitochondria, so that the defects in mitochondrial protein import via the TIM23 complex were partially recovered. Deletion of the ART5 gene in turn exhibited synthetic growth defects with the TAM41 deletion. Art5 as a functional partner for Tam41 will provide a starting point to reveal the precise function of Tam41 in the maintenance of the TIM23 complex.
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, and Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Harada
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013489:Suppression, Genetic
chemical_list: C547820:Art5 protein, S cerevisiae; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C514109:TAM41 protein, S cerevisiae; C083081:TIM23 protein, S cerevisiae