[Anti-tumor compounds from submerger fermentation products of a Paecilomyces militaris strain].
OBJECTIVE
The aim was to identify the antitumor compounds of the fermentation broth of Paecilomyces militaris (strain RCEF0927).


METHODS
Anti-tumor activity was tested with a resazurin cytotoxicity assay model with Chinese hamster ovary (CHO) cells. Bioactive compounds were analysed with combined method of High Performance Liquid Chromatography (HPLC), High Resolution Mass Spectrometer (HRMS) and antitumor assay.


RESULTS
Antitumor test showed that the fermentation broth had strong cytotoxicity against CHO cells. Extraction tests showed that ethylacetate was the best solvent for the bioactive constituents extracting. HPLC-DAD- HRMS-Cytotoxicity assay revealed that the molecular formula of the active compounds in the extract were possibly C10 H13 N5 O3, C22 H22 O9, C23 H24 O10, C22 H22 O10, C15 H10 O4, C15 H10O, C36 H70 O11, C36 H68 O12, C38 H74 O11, C40 H76 O13 and C44 H82 O14.


CONCLUSIONS
The possible structures of the bioactive compounds were deduced with bioassay, HRMS, Uv and database inquiry as cordycepin, daizein, genistein 7,4'-dihydroxyisoflavone-7-O-(4"-O-methyl)-b-D-glucopyranoside, 7, 4'-dihydroxy-6- methoxyisoflavone-7-O-( 4"-O-methyl)-b-D-glucopyranoside, 5, 7, 4'-trihydroxy-isoflavone-7-O-(4"-O-methyl)-b-D-glucopyranoside, polyoxyethylene ether oleate, dehydro products of polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxypropylene or polyoxybutene contained derivants of polyoxyethylene sorbitan fatty acid ester. Cytotoxicity of the compounds was revealed for the first time.
Lu
Ruili
R
Applied Chemistry Department of Science College, Anhui Agricultural University, Hefei 20036, China.
Hu
Fenglin
F
Huang
Bo
B
Li
Chunru
C
Li
Zengzhi
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D046508:Culture Techniques; D005285:Fermentation; D010143:Paecilomyces / Q000737:chemistry / Q000378:metabolism*
D000970:Antineoplastic Agents