Visinin-like protein 1 regulates natriuretic peptide receptor B in the heart.
Accumulating evidence indicates that Visinin-like protein-1 (VILIP-1), a member of the family of neuronal calcium sensor proteins (NCS), modulates a variety of processes in extra-neuronal tissues. In this study, we describe VILIP-1 expression in the human heart, rat cardiomyocytes, and H9c2 cells, and demonstrate that VILIP-1 regulates the cell surface localization of natriuretic peptide receptor B (NPR-B). In preparations from failing hearts, we observed VILIP-1 downregulation and reduced NPR-B signalling. In conclusion, VILIP-1 deficiency may be responsible for the reduced efficiency of the natriuretic peptide system in cardiac hypertrophy and heart failure and may therefore serve as pharmacological target.
Buttgereit
Jens
J
Max-Delbrück-Center for Molecular Medicine (MDC), Campus Berlin-Buch, Berlin, Germany. jens.buttgereit@charite.de
Qadri
Fatimunnisa
F
Monti
Jan
J
Langenickel
Thomas H
TH
Dietz
Rainer
R
Braunewell
Karl-Heinz
KH
Bader
Michael
M
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006439:Hemodynamics; D006801:Humans; D007150:Immunohistochemistry; D009203:Myocardial Infarction / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051596:Neurocalcin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D000099242:Atrial Natriuretic Factor Receptor B
D051596:Neurocalcin; D034741:RNA, Small Interfering; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B