An iron-based cytosolic catalase and superoxide dismutase mimic complex.
The development of metallodrugs with antioxidant activities is of importance as a way to protect organisms exposed to stressful conditions. Although iron chemistry in the presence of H(2)O(2) is usually associated with pro-oxidant activity, mainly via the Fenton reaction, we found that the mononuclear compound [Fe(HPClNOL)Cl(2)]NO(3) (1; C(15)H(18)Cl(3)FeN(4)O(4), a = 8.7751(3) A, b = 9.0778(4) A, c = 24.3869(10) A, beta = 93.370(2) degrees , monoclinic, P2(1)/c, Z = 4), containing the tripodal ligand 1-[bis(pyridin-2-ylmethyl)amino]-3-chloropropan-2-ol, decomposes hydrogen peroxide and superoxide anion in vitro as well as shows in vivo protection because it prevents the harmful effects promoted by H(2)O(2) on Saccharomyces cerevisiae cells, decreasing the level of lipid peroxidation. This protective effect was observed for wild-type cells, as well as for mutant cells, which do not present the antioxidant metalloenzymes catalase (Ctt1) or copper/zinc superoxide dismutase (Sod1).
Horn
Adolfo
A
Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, RJ, Brazil 28013-604. adolfo@uenf.br
Parrilha
Gabrieli L
GL
Melo
Karen V
KV
Fernandes
Christiane
C
Horner
Manfredo
M
Visentin
Lorenzo do C
Ldo C
Santos
Jullyane A S
JA
Santos
Monique S
MS
Eleutherio
Elis C A
EC
Pereira
Marcos D
MD
D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D015394:Molecular Structure; D018384:Oxidative Stress; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism
D000975:Antioxidants; D008024:Ligands; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase