Metal free, "click and click-click" conjugation of ribonucleosides and 2'-OMe oligoribonucleotides on the solid phase.
A fast and practical metal free conjugation of ribonucleosides and 2'-OMe 4-mer oligoribonucleotides has been accomplished by a nitrile oxide alkyne click cycloaddition reaction on the solid-phase, the methodology is suited to modification at either, or both, the 3'- or the 5'-terminus of the oligoribonucleotide substrate.
Singh
Ishwar
I
Department of Chemistry, NUI Maynooth, Maynooth, Co. Kildare, Republic of Ireland.
Heaney
Frances
F
D002851:Chromatography, High Pressure Liquid; D008670:Metals / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D012263:Ribonucleosides / Q000737:chemistry*
D008670:Metals; D009843:Oligoribonucleotides; D012263:Ribonucleosides; D012313:RNA