title: Proteomic analysis of embryonic Fasciola hepatica: characterization and antigenic potential of a developmentally regulated heat shock protein.
abstract: Fasciola hepatica is responsible for human disease and economic livestock loss on a global scale. We report the first post-genomic investigation of cellular proteins expressed by embryonic F. hepatica via two-dimensional electrophoresis, image analysis and tandem mass spectrometry. Antioxidant proteins and protein chaperones are prominently expressed by embryonic F. hepatica. Molecular differences between the egg and other characterized F. hepatica lifecycle stages were noted. Furthermore, proteins expressed within liver fluke eggs differ to those isolated from the well-characterized eggs of the human blood flatworm Schistosoma mansoni were revealed. Plasticity in expression of major proteins, particularly a prominently expressed 65kDa protein cluster was seen between natural populations of embryonating F. hepatica eggs suggesting that liver fluke embryogenisis is a plastic process. Immunoblotting revealed that the abundant 65kDa protein cluster is recognised by infection sera from three F. hepatica challenged host species. Mass spectrometry and BLAST analyses demonstrated that the 65kDa antigen shows homology to egg antigens of other flatworm parasites, and is represented in a F. hepatica EST database constructed from adult fluke transcripts. EST clones encoding the egg antigen were re-sequenced, predicting two forms of the protein. Four clones predict a 312 aa polypeptide, three clones encode a putative 110 amino acid extension at the N-terminus which may be involved in protein secretion, although this extension was not expressed by natively extracted proteins. Consistent expression of alpha crystallin domains confirmed the protein to be a member of the alpha crystallin containing small heat shock protein (AC/sHSP) superfamily. AC/sHSPs are ubiquitous in nature, however, this is the first time a member of this protein superfamily has been described from F. hepatica. The antigenic AC/sHSP was named Fh-HSP35alpha based on predictions of molecular weight. Production of recombinant Fh-HSP35alpha reveals considerable mass discrepancy between native and recombinant proteins, although descriptions of other characterized flatworm AC/sHSPs, suggest that the native form is a dimer. Immunoblot analyses confirm that the recombinant protein is recognised by F. hepatica challenged hosts, but does not react with sera from non-infected animals. We discuss the potential of recombinant Fh-HSP35alpha as an egg-based diagnostic marker for liver fluke infection.
authors.affiliation: Institute of Biological, Environmental and Rural Sciences (IBERS), Edward Llwyd Building, Aberystwyth University, UK. joseph.moxon@jcu.edu.au
authors.forename: Joseph V
authors.initials: JV
authors.lastname: Moxon
authors.forename: E James
authors.initials: EJ
authors.lastname: LaCourse
authors.forename: Hazel A
authors.initials: HA
authors.lastname: Wright
authors.forename: Samirah
authors.initials: S
authors.lastname: Perally
authors.forename: Mark C
authors.initials: MC
authors.lastname: Prescott
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Gillard
authors.forename: John
authors.initials: J
authors.lastname: Barrett
authors.forename: Joanne V
authors.initials: JV
authors.lastname: Hamilton
authors.forename: Peter M
authors.initials: PM
authors.lastname: Brophy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000378:metabolism*; D005210:Fasciola hepatica / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D040901:Proteomics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D015053:Zygote / Q000254:growth & development
chemical_list: D000947:Antigens, Helminth; D006360:Heat-Shock Proteins