Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticles.
PEGylated gold nanoparticles are decorated with various amounts of human transferrin (Tf) to give a series of Tf-targeted particles with near-constant size and electrokinetic potential. The effects of Tf content on nanoparticle tumor targeting were investigated in mice bearing s.c. Neuro2A tumors. Quantitative biodistributions of the nanoparticles 24 h after i.v. tail-vein injections show that the nanoparticle accumulations in the tumors and other organs are independent of Tf. However, the nanoparticle localizations within a particular organ are influenced by the Tf content. In tumor tissue, the content of targeting ligands significantly influences the number of nanoparticles localized within the cancer cells. In liver tissue, high Tf content leads to small amounts of the nanoparticles residing in hepatocytes, whereas most nanoparticles remain in nonparenchymal cells. These results suggest that targeted nanoparticles can provide greater intracellular delivery of therapeutic agents to the cancer cells within solid tumors than their nontargeted analogs.
Choi
Chung Hang J
CH
Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Alabi
Christopher A
CA
Webster
Paul
P
Davis
Mark E
ME
D000818:Animals; D001667:Binding, Competitive; D016503:Drug Delivery Systems; D005260:Female; D006046:Gold / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry; D053768:Metal Nanoparticles; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism; D010316:Particle Size; D014168:Transferrin / Q000008:administration & dosage* / Q000493:pharmacokinetics
D014168:Transferrin; D006046:Gold