Long-term and short-term effects of simulated birth trauma, cesarean and vaginal delivery on sulfated glycosaminoglycans in the urethra of female rats.
OBJECTIVE
This study assesses the effects of simulated birth trauma, vaginal and cesarean delivery on glycosaminoglycans in the connective tissue of female rat urethra.


METHODS
One hundred and ten female rats were divided into six groups: A--control, B--vaginal trauma, C--cesarean delivery, D--cesarean delivery followed by vaginal trauma, E--vaginal delivery, and F--female rats on the 20th day of gestation. In each group, half of the animals were sacrificed 4 days after the procedures and half were sacrificed 12 weeks later. Glycosaminoglycans were extracted by proteolysis and analyzed using densitometry after agarose gel electrophoresis.


RESULTS
The examination at 4 days showed larger amounts of glycosaminoglycans in pregnant female rats compared to other groups. The examination at 12 weeks showed larger amounts of glycosaminoglycans in the cesarean delivery group followed by simulated birth trauma.


CONCLUSIONS
There is a change in sulfated glycosaminoglycans in female rat urethra during pregnancy and simulated birth trauma.
Takano
Claudia Cristina
CC
Section of Urogynecology and Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, Rua Capitão Rosendo, 100 apart 54b Vila Mariana, CEP 04120-060 São Paulo, Brazil. claudia.takano@uol.com.br
Bezerra
Leonardo Robson Pinheiro Sobreira
LR
Zucchi
Eliana Viana Monteiro
EV
Nader
Helena Bonciani
HB
Sartori
Marair Gracio Ferreira
MG
Girão
Manoel João Batista Castelo
MJ
D000818:Animals; D002585:Cesarean Section / Q000009:adverse effects*; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D036801:Parturition; D049590:Postpartum Period; D011247:Pregnancy; D051381:Rats; D013997:Time Factors; D014521:Urethra / Q000293:injuries* / Q000378:metabolism*; D014621:Vagina / Q000293:injuries
D006025:Glycosaminoglycans