[The genetic characterization of enteric cytopathic human orphanviruses type 13 isolated in Shandong province].
OBJECTIVE
To analysis the genetic characteristics of Echo 13 isolated from stool specimens of AFP patient in Shandong.


METHODS
The complete VP1 sequences of the 6 isolates were successfully amplified by RT-PCR and were compared with other Echovirus 13 isolates available from GenBank.


RESULTS
6 Shandong isolates were clearly identified as Echo 13 and were grouped into B cluster that agreed with neutralization result. The homogeneity analysis showed that the Shandong isolates share at least 80% similarity in nucleotide level and 94.4% in amino acid level. Phylogenetic tree base on complete VP1 sequence indicated there were many transmitting chains of Echovirus 13 prevailed in China.


CONCLUSIONS
All Echo 13 isolates were divided into three genomic clusters A, B and C. It is suggested that enterovirus surveillance should be strengthened to prevent imported of Echo 13.
Mao
Nai-ying
NY
World Health Organization Western Pacific Region Regional Reference Measles Laboratory, State Key Laboratory for Molecular & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wang
Hai-yan
HY
Zhu
Zhen
Z
D002460:Cell Line; D002681:China / Q000453:epidemiology; D003588:Cytopathogenic Effect, Viral; D004457:Echovirus Infections / Q000453:epidemiology / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny