title: Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish.
abstract: Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835(T) and JF4413, produced non-diffusible green pigment following 4-5 days incubation on Luria-Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C(16 : 1)ω7c and/or C(15 : 0) iso 2-OH), C(16 : 0) and C(18 : 1)ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100 % sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84 %), P. chlororaphis subsp. aurantiaca (99.75 %) and P. chlororaphis subsp. aureofaciens (99.40 %). While DNA-DNA relatedness confirmed the placement of strains JF3835(T) and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835(T) and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835(T) (=NCIMB 14478(T)=DSM 21509(T)).
authors.affiliation: Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Burr
authors.affiliation: Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Gobeli
authors.affiliation: Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.affiliation: Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Elinor
authors.initials: E
authors.lastname: Goldschmidt-Clermont
authors.affiliation: Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005399:Fishes / Q000382:microbiology*; D005618:Fresh Water; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013557:Switzerland
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S