[D9 measles virus was first isolated from an imported measles case in Sichuan Province of China].
OBJECTIVE
To isolate and identify the genotype and molecular characteristic of an imported D9 measles virus.


METHODS
To isolate the virus with Vero/SLAM cell line. And RT-PCR (reverse transcription-polymerase chain reaction) were performed to amplify the 450 nucleotide acids of carboxyl terminal of N (nucleoprotein, N) protein. The phylogenetic tree was constructed and the homology similarity was analyzed.


RESULTS
Sichuan isolate MVi/Sichuan.CHN/07.09/1 was clustered within the same genotype group with WHO D9 genotype reference strain, the homology of nucleotide acid between Sichuan isolate and WHO D9 genotype reference strain was 96.9%. The homology of nucleotide acid and amino acid between Sichuan isolate and 2008 D9 genotype representative strain were 99.8%-100% and 99.3%-100% respectively. Compared with the Chinese measles vaccine strain, the homology of nucleotide acid and amino acid of Shanghai-191 were 92.3% and 90.7% respectively. Compared with the endemic H1 genotype representative measles strain, the homology of nucleotide acid and amino acid were 90.8% and 92.1% respectively.


CONCLUSIONS
The imported virus was D9 genotype measles virus.
Zhang
Yan
Y
Institute for Piral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
He
Ji-Lan
JL
Sun
Li
L
D000818:Animals; D002681:China; D002522:Chlorocebus aethiops; D005260:Female; D005838:Genotype; D006801:Humans; D008457:Measles / Q000821:virology*; D008459:Measles virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014195:Travel; D014709:Vero Cells