Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food.
We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin.
Pafundo
Simona
S
Department of Environmental Sciences, Division of Genetics and Environmental Biotechnology, University of Parma, Viale G.P. Usberti 11/A, 43100, Parma, Italy.
Gullì
Mariolina
M
Marmiroli
Nelson
N
D000485:Allergens / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D005502:Food; D005504:Food Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D000485:Allergens; D005456:Fluorescent Dyes; D004247:DNA