A one-step modified method to reduce the burst initial release from PLGA microspheres.
To decrease the initial burst release of protein entrapped in PLGA microspheres, bovine serum albumin was entrapped into microspheres and a one-step modified method was evaluated. Low level of alginate was added into the internal aqueous phase with protein together; meanwhile calcium chloride and chitosan were put into the external, and all the other processes kept unvaried. After modification, the initial release was inhibited markedly, and the entrapment efficiency was increased slightly, while the particle size remained unaltered. An attracting result is that almost all the little pores at the surface of microspheres had been closed with the modification one under SEM.
Zheng
Caihong
C
Women's Hospital Affiliated College of Medical Sciences, Zhejiang University, Hangzhou 310006, PR China. chzheng@zju.edu.cn
Liang
Wenquan
W
D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000008:administration & dosage; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry; D017220:Dentin-Bonding Agents; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000379:methods*; D005079:Excipients / Q000008:administration & dosage; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008689:Methacrylates / Q000008:administration & dosage*; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D013499:Surface Properties
D000464:Alginates; D001672:Biocompatible Materials; C103506:Bis-GMA, BPDM, HEMA dental-bonding resin; D017220:Dentin-Bonding Agents; D004337:Drug Carriers; D005079:Excipients; D006603:Hexuronic Acids; D008689:Methacrylates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D020723:Glucuronic Acid; D048271:Chitosan