Soluble, folded and active subtilisin in a protic ionic liquid.
The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.
Falcioni
Francesco
F
CNAP, Department of Biology, University of York, York, United Kingdom YO10 5YW.
Housden
Hazel R
HR
Ling
Zhenlian
Z
Shimizu
Seishi
S
Walker
Adam J
AJ
Bruce
Neil C
NC
D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D052578:Ionic Liquids / Q000737:chemistry*; D017510:Protein Folding; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012995:Solubility; D020860:Subtilisin / Q000737:chemistry / Q000378:metabolism*
D052578:Ionic Liquids; D000644:Quaternary Ammonium Compounds; D002918:Chymotrypsin; D020860:Subtilisin