Expression of extracellular matrix proteins in adenomatoid odontogenic tumor.
Altered expression of extracellular matrix (ECM) components has been reported in several pathologies; however, few ECM proteins have been evaluated in adenomatoid odontogenic tumor (AOT). The aim of this study was to analyze the expression and distribution of the ECM proteoglycans: biglycan and decorin; and glycoproteins: osteonectin, osteopontin, bone sialoprotein and osteocalcin in the AOT. Three-micrometer sections from paraffin-embedded specimens were evaluated employing a streptavidin-biotin immunohistochemical method with the antibodies against the proteins previously cited. Only the osteonectin was expressed in the epithelial cells. The eosinophilic amorphous material and the connective tissue showed expression of all components studied. The calcification foci expressed only osteopontin. In conclusion, the low expression of the components studied in neoplastic epithelial cells suggests that the epithelial cells act probably as stimulators of the expression by the stroma, which in turn can act as agonist or antagonist of the tumor growth. These results suggest that the components studied probably have a key role in the biological behavior of the AOT.
Modolo
Filipe
F
Pathology Department, Health Sciences Center, Federal University of Santa Catarina, Florianopolis. modolo@ccs.ufsc.br
Biz
Michelle Tillmann
MT
Martins
Marília Trierveiller
MT
Machado de Sousa
Suzana Orsini
SO
de Araújo
Ney Soares
NS
D058578:Biglycan; D002114:Calcinosis / Q000473:pathology; D003238:Connective Tissue / Q000473:pathology; D003593:Cytoplasm / Q000648:ultrastructure; D058575:Decorin; D004847:Epithelial Cells / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D009808:Odontogenic Tumors / Q000473:pathology*; D015675:Osteocalcin / Q000032:analysis; D015676:Osteonectin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D011509:Proteoglycans; D012795:Sialoglycoproteins; D015675:Osteocalcin; D053495:Osteopontin