Vaccination with TCL plus MHSP65 induces anti-lung cancer immunity in mice.
To develop effective anti-lung cancer vaccines, we directly mixed mycobacterial heat shock protein 65 (MHSP65) and tumor cell lysate (TCL) from Lewis lung cancer cells in vitro and tested its efficacy on stimulating anti-tumor immunity. Our results showed that MHSP65-TCL immunization significantly inhibited the growth of lung cancer in mice and prolonged the survival of lung cancer bearing mice. In vivo and in vitro data suggest that MHSP65-TCL could induce specific CTL responses and non-specific immunity, both of which could contribute to the tumor inhibition. Thus, this report provides an easy approach to prepare an efficient TCL based tumor vaccine.
Dong
Bohan
B
Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China.
Sun
Luguo
L
Wu
Xiuli
X
Zhang
Peiyin
P
Wang
Li
L
Wei
Hongfei
H
Zhou
Lei
L
Hu
Xiaoping
X
Yu
Yongli
Y
Hua
Shucheng
S
Wang
Liying
L
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology; D019496:Cancer Vaccines; D018827:Carcinoma, Lewis Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002457:Cell Extracts / Q000008:administration & dosage / Q000276:immunology; D048708:Cell Growth Processes / Q000187:drug effects / Q000276:immunology; D018834:Chaperonin 60 / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D047368:Tumor Burden / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D019496:Cancer Vaccines; D002457:Cell Extracts; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium