Mass spectrometric identification of aberrantly glycosylated human apolipoprotein C-III peptides in urine from Schistosoma mansoni-infected individuals.
Schistosomiasis is a parasitic infection caused by Schistosoma flatworms, prime examples of multicellular parasites that live in the mammalian host for many years. Glycoconjugates derived from the parasite have been shown to play an important role in many aspects of schistosomiasis, and some of them are present in the circulation of the host. The aim of this study was to identify novel glycoconjugates related to schistosomiasis in urine of Schistosoma mansoni-infected individuals using a combination of glycopeptide separation techniques and in-depth mass spectrometric analysis. Surprisingly, we characterized a heterogeneous population of novel aberrantly O-glycosylated peptides derived from the C terminus of human apolipoprotein C-III (apoC-III) in urine of S. mansoni-infected individuals that were not detected in urine of non-infected controls. The glycan composition of these glycopeptides is completely different from what has been described previously for apoC-III. Most importantly, they lack sialylation and display a high degree of fucosylation. This study exemplifies the potential of mass spectrometry for the identification and characterization of O-glycopeptides without prior knowledge of either the glycan or the peptide sequence. Furthermore, our results indicate for the first time that as a result of S. mansoni infection the glycosylation of a host protein is altered.
Balog
Crina I A
CI
Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. C.I.A.Balog@lumc.nl
Mayboroda
Oleg A
OA
Wuhrer
Manfred
M
Hokke
Cornelis H
CH
Deelder
André M
AM
Hensbergen
Paul J
PJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D053305:Apolipoprotein C-III / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000652:urine*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006031:Glycosylation; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D012550:Schistosoma mansoni / Q000378:metabolism / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000378:metabolism / Q000652:urine*; D055815:Young Adult
D053305:Apolipoprotein C-III; D010446:Peptide Fragments