Enhancing the therapeutic effect against ovarian cancer through a combination of viral oncolysis and antigen-specific immunotherapy.
Cancer therapy using oncolytic viruses represents a promising new approach for controlling ovarian cancer. In this study, we have circumvented the limitation of repeated vaccination by employing different virus vectors, Semliki Forest Virus (SFV) and vaccinia virus (VV) for boosting the immune response. We found that infection of tumor-bearing mice with VV followed by infection with SFV or vice versa leads to enhanced antitumor effects against murine ovarian surface epithelial carcinoma (MOSEC) tumors. Furthermore, infection with VV-ovalbumin (OVA) followed by infection with SFV-OVA or vice versa was found to lead to enhanced OVA-specific CD8(+) T-cell immune responses. In addition, we found that infection with SFV-OVA followed by infection with VV-OVA leads to enhanced antitumor effects in vivo and enhanced tumor killing in vitro through a combination of viral oncolysis and antigen-specific immunity. The clinical implications of this study are discussed.
Zhang
Yu-Qian
YQ
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA.
Tsai
Ya-Chea
YC
Monie
Archana
A
Wu
T-C
TC
Hung
Chien-Fu
CF
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D007167:Immunotherapy; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D050130:Oncolytic Virotherapy; D010051:Ovarian Neoplasms / Q000276:immunology / Q000628:therapy*; D012672:Semliki forest virus / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology
D000951:Antigens, Neoplasm; D007371:Interferon-gamma