Extensive screening system using suspension array technology to detect mitochondrial DNA point mutations.
We established an extensive and rapid system using suspension array to detect 61 representative mitochondrial DNA (mtDNA) heteroplasmic or homoplasmic point mutations (29 for Series A and 32 for Series B) in 22 genes: 1 each in MT-RNR1, -TV, -ND1, -TQ, -TW, -TC, and -TH genes; 2 each in MT-TN, -TG, -ND4, -TL2, -TE, and -CYB genes; 3 each in MT-ATP6, -ND3, and -ND5 genes; 4 each in MT-CO1 and -TK genes; 5 each in MT-TI, -TS1, and -ND6 genes; and 10 in the MT-TL1 gene. We carefully selected 5'-biotinylated primers and pooled primers for use in two sets of multiplex-PCR amplifications. To detect both mutant and wild-type mtDNA, even when polymorphisms were present near the target mutation sites, we designed specific oligonucleotide probes. By using the mtDNA point mutation detection system of Series A (29 mutations) and Series B (32 mutations), we screened a total of 3103 mutant sites in 107 DNA samples for Series A and 13,101 mutant sites in 397 DNA samples for Series B. We succeeded in determining 99.4% (Series A) and 99.6% (Series B) of the targeted mutant sites by use of the system. The 22 samples with the m.3243A>G heteroplasmic mutation revealed positive signals with both mutant- and wild-type-specific probes in this detection system with a detection limit of approximately 2%. This genetic screening platform is useful to reach a definitive diagnosis for mitochondrial diseases.
Nishigaki
Yutaka
Y
Department of Genomics for Longevity and Health, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan. yutakan@tmig.or.jp
Ueno
Hitomi
H
Coku
Jorida
J
Koga
Yasutoshi
Y
Fujii
Tatsuya
T
Sahashi
Ko
K
Nakano
Kazutoshi
K
Yoneda
Makoto
M
Nonaka
Michiko
M
Tang
Linya
L
Liou
Chia-Wei
CW
Paquis-Flucklinger
Veronique
V
Harigaya
Yasuo
Y
Ibi
Tohru
T
Goto
Yu-ichi
Y
Hosoya
Hiroko
H
DiMauro
Salvatore
S
Hirano
Michio
M
Tanaka
Masashi
M
D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial