Effect of KIOM-79 against mitochondrial damage induced by streptozotocin in pancreatic beta-cells.
The present study examined the effects of KIOM-79 on streptozotocin (STZ)-induced mitochondrial oxidative stress in rat pancreatic beta-cells (RINm5F). KIOM-79 is a mixture of plant extracts from parched Puerariae radix, gingered Magnoliae cortex, Glycyrrhizae radix, and Euphorbiae radix. A marked increase in mitochondrial reactive oxygen species (ROS) was observed in STZ induced diabetic cells, which returned to control conditions after KIOM-79 treatment. Mitochondrial manganese superoxide dismutase (Mn SOD) activity and its protein expression were downregulated by STZ treatment but upregulated by KIOM-79 treatment. In addition, KIOM-79 treatment restored the loss of the mitochondrial membrane potential (Deltapsi) produced by STZ treatment. KIOM-79 induced an increase in Bcl-2 and a decrease in phospho Bcl-2 and Bax, which are related to permeability of the mitochondrial membrane. Further, KIOM-79 inhibited the translocation of cytochrome c from the mitochondria to the cytosol and elevated the ATP level, which was reduced by STZ treatment. These results suggest that KIOM-79 exhibits a protective effect through activation of antioxidant defense mechanisms and by attenuation of mitochondrial dysfunction in STZ-induced diabetic cells.
Kang
Kyoung Ah
KA
School of Medicine, Jeju National University, Jeju-si, Korea.
Kim
Jin Sook
JS
Zhang
Rui
R
Piao
Mei Jing
MJ
Ko
Dong Ok
DO
Wang
Zhi Hong
ZH
Heo
Young Jun
YJ
Park
Doek Bae
DB
Maeng
Young Hee
YH
Hyun
Jin Won
JW
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013311:Streptozocin / Q000633:toxicity*
C517978:KIOM 79; D010936:Plant Extracts; D013311:Streptozocin; D000255:Adenosine Triphosphate