title: Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques.
abstract: Here we describe a novel vaccine vector for expressing human immunodeficiency virus (HIV) antigens. We show that recombinant attenuated yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 Gag sequences can be used as a vector to generate SIV-specific CD8(+) T-cell responses in the rhesus macaque. Priming with recombinant BCG expressing SIV antigens increased the frequency of these SIV-specific CD8(+) T-cell responses after recombinant YF17D boosting. These recombinant YF17D-induced SIV-specific CD8(+) T cells secreted several cytokines, were largely effector memory T cells, and suppressed viral replication in CD4(+) T cells.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Dr., Madison, WI 53711, USA.
authors.forename: Myrna C
authors.initials: MC
authors.lastname: Bonaldo
authors.forename: Mauricio A
authors.initials: MA
authors.lastname: Martins
authors.forename: Richard
authors.initials: R
authors.lastname: Rudersdorf
authors.forename: Philip A
authors.initials: PA
authors.lastname: Mudd
authors.forename: Jonah B
authors.initials: JB
authors.lastname: Sacha
authors.forename: Shari M
authors.initials: SM
authors.lastname: Piaskowski
authors.forename: Patrícia C
authors.initials: PC
authors.lastname: Costa Neves
authors.forename: Marlon G
authors.initials: MG
authors.lastname: Veloso de Santana
authors.forename: Lara
authors.initials: L
authors.lastname: Vojnov
authors.forename: Saverio
authors.initials: S
authors.lastname: Capuano
authors.forename: Eva G
authors.initials: EG
authors.lastname: Rakasz
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Wilson
authors.forename: John
authors.initials: J
authors.lastname: Fulkerson
authors.forename: Jerald C
authors.initials: JC
authors.lastname: Sadoff
authors.forename: David I
authors.initials: DI
authors.lastname: Watkins
authors.forename: Ricardo
authors.initials: R
authors.lastname: Galler
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D008253:Macaca mulatta; D010446:Peptide Fragments / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D022341:Yellow Fever Vaccine / Q000276:immunology; D015005:Yellow fever virus / Q000235:genetics*
chemical_list: D015683:Gene Products, gag; D010446:Peptide Fragments; D018449:SAIDS Vaccines; D014614:Vaccines, Synthetic; D022341:Yellow Fever Vaccine