Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.
PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.
Cnops
Lieselotte
L
Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Antwerp, Belgium. lcnops@itg.be
Esbroeck
Marjan Van
MV
D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D004747:Entamoeba / Q000235:genetics / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D005615:Freezing; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
D016054:DNA, Protozoan