title: Different levels of Hsp72 in female rat myocardium in response to voluntary exercise and forced exercise.
abstract: BACKGROUND
  Physical exercise induces hemodynamic stress.
  
  
  OBJECTIVE
  To evaluate if voluntary running and forced running induced different levels of stress protein (Hsp72) in the myocardium of female Wistar rats.
  
  
  METHODS
  Female rats were randomly assigned to the following groups: forced treadmill running group (FR; n= 6), voluntary running group (VR; n=6) and control group (C; n=6). VR group animals had free access to running wheels, and those from FR group underwent a running program on a treadmill (18 m/min, 60 min/day, 5 days/wk) for 8 weeks. Left ventricle (LV) and right ventricle (RV) fragments were collected at sacrifice, and the relative immunoblot contents of stress protein (Hsp72) were determined.
  
  
  RESULTS
  VR animals ran on average 4.87 km/wk, and FR rats ran 4.88 km/wk. Animals from VR and FR groups had less body weight gain (p<0.05) than those from C group (81.67 +/- 11.95g vs 81.17 +/- 10.18g vs 111.50 +/- 2.26g, respectively). Heart weight/body weight ratio was not significantly different (p>0.05) among VR, FR and C groups (4.54 +/- 0.79 mg/g vs 4.94 +/- 0.89 mg/g vs 4.34 +/- 0.87 mg/g, respectively). FR group animals had levels of Hsp72 (p<0.05) higher than those from VR, both in LV (287.45 +/- 35.86 % vs 135.59 +/- 5.10 %, respectively) and RV (241.31 +/- 25.83 % vs 137.91 +/- 45.20 %, respectively).
  
  
  CONCLUSIONS
  Voluntary running and forced running induced different levels of Hsp72 in the myocardium of female Wistar rats.
authors.affiliation: Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Stéphano Freitas Soares
authors.initials: SF
authors.lastname: Melo
authors.forename: Wellington
authors.initials: W
authors.lastname: Lunz
authors.forename: Elizabeth Pacheco Batista
authors.initials: EP
authors.lastname: Fontes
authors.forename: Cristina Maria Ganns Chaves
authors.initials: CM
authors.lastname: Dias
authors.forename: Miguel Araujo
authors.initials: MA
authors.lastname: Carneiro
authors.forename: Anselmo Gomes de
authors.initials: AG
authors.lastname: Moura
authors.forename: Ricardo Junqueira
authors.initials: RJ
authors.lastname: Del Carlo
authors.forename: Antonio Jose
authors.initials: AJ
authors.lastname: Natali
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D004812:Epidemiologic Methods; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D006352:Heart Ventricles / Q000473:pathology; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012420:Running / Q000502:physiology*
chemical_list: D050884:HSP72 Heat-Shock Proteins