title: Preferential transfer of certain plasma membrane proteins onto T and B cells by trogocytosis.
abstract: T and B cells capture antigens via membrane fragments of antigen presenting cells (APC) in a process termed trogocytosis. Whether (and how) a preferential transfer of some APC components occurs during trogocytosis is still largely unknown. We analyzed the transfer onto murine T and B cells of a large panel of fluorescent proteins with different intra-cellular localizations in the APC or various types of anchors in the plasma membrane (PM). Only the latter were transferred by trogocytosis, albeit with different efficiencies. Unexpectedly, proteins anchored to the PM's cytoplasmic face, or recruited to it via interaction with phosphinositides, were more efficiently transferred than those facing the outside of the cell. For proteins spanning the PM's whole width, transfer efficiency was found to vary quite substantially, with tetraspanins, CD4 and FcRgamma found among the most efficiently transferred proteins. We exploited our findings to set immunodiagnostic assays based on the capture of preferentially transferred components onto T or B cells. The preferential transfer documented here should prove useful in deciphering the cellular structures involved in trogocytosis.
authors.affiliation: CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Daubeuf
authors.forename: Anne
authors.initials: A
authors.lastname: Aucher
authors.forename: Christine
authors.initials: C
authors.lastname: Bordier
authors.forename: Audrey
authors.initials: A
authors.lastname: Salles
authors.forename: Laurent
authors.initials: L
authors.lastname: Serre
authors.forename: Gérald
authors.initials: G
authors.lastname: Gaibelet
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Faye
authors.forename: Gilles
authors.initials: G
authors.lastname: Favre
authors.forename: Etienne
authors.initials: E
authors.lastname: Joly
authors.forename: Denis
authors.initials: D
authors.lastname: Hudrisier
mesh_terms: D017951:Antigen Presentation; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D021381:Protein Transport / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D008565:Membrane Proteins