title: A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles.
abstract: The effects of titanium dioxide (TiO(2)) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO(2) NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO(2) NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO(2) NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphorylation, which is negatively regulated by the protein kinase C epsilon (PKCepsilon), is demonstrated. It is inferred that PKCepsilon plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO(2) NPs exposure.
authors.affiliation: Laboratory of Molecular Neurobiology, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Xiufang
authors.initials: X
authors.lastname: Ren
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Deng
authors.forename: Yinan
authors.initials: Y
authors.lastname: Huo
authors.forename: Jiang
authors.initials: J
authors.lastname: Xie
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiao
authors.forename: Minghong
authors.initials: M
authors.lastname: Wu
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Tieqiao
authors.initials: T
authors.lastname: Wen
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D025941:Protein Interaction Mapping / Q000379:methods; D020543:Proteome / Q000032:analysis; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D018031:Connexin 43; D020543:Proteome; C009495:titanium dioxide; D014025:Titanium