title: Counting single molecules in sub-nanolitre droplets.
abstract: We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.
authors.affiliation: Johns Hopkins University, Department of Biomedical Engineering, 3400 N. Charles St., Clark Hall, Baltimore, MD, USA. tushar@jhu.edu
authors.forename: T D
authors.initials: TD
authors.lastname: Rane
authors.forename: C M
authors.initials: CM
authors.lastname: Puleo
authors.forename: K J
authors.initials: KJ
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: A P
authors.initials: AP
authors.lastname: Lee
authors.forename: T H
authors.initials: TH
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000032:analysis* / Q000648:ultrastructure; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000032:analysis* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
chemical_list: D012996:Solutions; D004247:DNA