title: Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1.
abstract: In this study, we obtained evidence indicating that annexin 1 is a new target of the p38/MAPKAP kinase-2 pathway and that it regulates endothelial cell migration in response to vascular endothelial growth factor (VEGF). These conclusions are supported by a series of substantiating experiments. First, by two-dimensional gel electrophoresis and mass spectrometry, we identified annexin 1 as a protein whose phosphorylation is induced by VEGF and is impaired by inhibiting p38. Second, using in vitro kinase assays and in vivo phosphorylation assays, we found that VEGF-mediated activation of LIM kinase 1 downstream of the p38 pathway triggers the phosphorylation of annexin 1. Third, VEGF-induced cell migration and tube formation in Matrigel are inhibited following small interfering RNA-mediated knockdown of annexin 1. Fourth, both processes are rescued in cells expressing an annexin 1 construct insensitive to the small interfering RNA knockdown. Finally, the VEGF/annexin 1-mediated cell migration is impaired by inhibiting p38. We therefore conclude that phosphorylation of annexin 1 regulates the angiogenic effect that is associated with the activation of the p38/LIM kinase 1 axis by VEGF.
authors.affiliation: Cancer Research Center, Laval University, Québec G1R-2J6, Canada.
authors.forename: Maxime C
authors.initials: MC
authors.lastname: Côté
authors.forename: Jessie R
authors.initials: JR
authors.lastname: Lavoie
authors.forename: François
authors.initials: F
authors.lastname: Houle
authors.forename: Andrée
authors.initials: A
authors.lastname: Poirier
authors.forename: Simon
authors.initials: S
authors.lastname: Rousseau
authors.forename: Jacques
authors.initials: J
authors.lastname: Huot
mesh_terms: D017305:Annexin A1 / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007797:Laminin; D054461:Lim Kinases / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D013058:Mass Spectrometry; D018919:Neovascularization, Physiologic / Q000502:physiology; D010766:Phosphorylation / Q000502:physiology; D011509:Proteoglycans; D034741:RNA, Small Interfering; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D017305:Annexin A1; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D034741:RNA, Small Interfering; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C059485:matrigel; D003094:Collagen; C515892:LIMK1 protein, human; D054461:Lim Kinases; D048051:p38 Mitogen-Activated Protein Kinases