Effect of hemolysis on insulin determination by the Beckman Coulter Unicell DXI 800 immunoassay analyzer.
OBJECTIVE
To evaluate the effect of hemolysis on the measurement of insulin using the Beckman Coulter Unicell DXI 800 immunoassay analyzer.


METHODS
Insulin concentrations were measured using the Beckman Coulter Unicell DXI 800. Interference studies were conducted by mixing plasma of known insulin concentrations with hemolysates of increasing hemoglobin concentration. Hemolysates were prepared by osmotic shock. A change of >10% from baseline results was taken as evidence of significant interference.


RESULTS
Hemolysates mediated a concentration-dependent reduction in insulin concentration.


CONCLUSIONS
A concentration-dependent negative bias in the measurement of insulin is associated with increasing degrees of hemolysis on the Beckman Coulter Unicell DXI 800.
Cook
Paul R
PR
Southampton University Hospitals NHS Trust, Clinical Pathology Care Group, Division of Diagnostics & Therapy, Clinical Biochemistry, Level D, South Block, Mailpoint 6, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom. paul.cook@suht.swest.nhs.uk
Glenn
Christine
C
Armston
Annie
A
D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D001793:Blood Preservation / Q000009:adverse effects; D001800:Blood Specimen Collection / Q000379:methods; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007328:Insulin / Q000032:analysis* / Q000097:blood; D009994:Osmolar Concentration
D006454:Hemoglobins; D007328:Insulin