[The effect of PTC1 transfection on the growth and apoptosis of human pulmonary adenocarcinoma cell A549].
OBJECTIVE
To investigate the effects of the tumor suppressor gene PTC on the growth inhibition and down-regulation of epidermal growth factor receptors (EGFR) in pulmonary adenocarcinoma cell A549.


METHODS
Pulmonary adenocarcinoma cell A549 were divided into wild type group, mutant type group, blank group and control group. They were transfected with wild-type PTC1 plasmids, mutant-PTC1 plasmids and blank plasmids, respectively. After transfection, the cell growth curve was drown every day for a week. The expression of PTC1 and EGFR were detected by western blot. Flow cytometry was used to analyze apoptosis and cell cycle of the transfected cells.


RESULTS
After transfected with wild-type PTC1, the growth rates of A549 cells were slow down, but the other groups of cells had no change. Compared with the control group, the expression of EGFR were down-regulated. The apoptosis rates in wild type group was 24.5%, and the mutant type group was 8.3% (P < 0.01). But the apoptosis rate of blank group has no change.


CONCLUSIONS
Wild-type PTC1 could induce apoptosis and inhibitory effect on A549 cells.
Song
Qi-bin
QB
Cancer Center of Renmin Hospital, Wuhan University, Wuhan 430060, China.
Hu
Wei-guo
WG
Yao
Yi
Y
Wang
Wei-xing
WX
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D000072079:Patched Receptors; D010957:Plasmids / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D000072079:Patched Receptors; D011956:Receptors, Cell Surface; D066246:ErbB Receptors