[The comparison of three cytogenetic methods for detecting multiple myeloma].
OBJECTIVE
To explore the value of conventional cytogenetic technique (CC), modified cell culture (long term culture and increasing the final concentration of colcemid) and fluorescence in situ hybridization (FISH) in detection of chromosomal and genomic aberrations of multiple myeloma(MM).


METHODS
RHG banding was used to evaluate the efficiency of modified culture method on abnormal karyotype detection in 21 MM patients. The probes 1q21, 13q14 (RB1), 14q32 (IGHC/IGHV gene) were used to perform FISH in the detection of chromosomal and genomic aberrations of MM.


RESULTS
Abnormal chromosomes were detected in 4 cases (19.1%) of the 21 MM patients with CC. After modified cell culture, abnormal karyotype was detected in 6 cases (28.6%). Abnormal chromosomes were detected in 12 of 18 cases (66.7%) using FISH. Panel FISH disclosed 1q21 amplification in 4 (22.2%), del(13q) abnormality in 5 (27.8%), 14q32 rearrangement in 8 (44.4%).


CONCLUSIONS
FISH can significantly improve the detection rate of chromosomal aberrations in MM.
Ren
Juan
J
Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
Yang
Lin-hua
LH
Li
Guo-xia
GX
Fan
Jian-ling
JL
Hou
Jian
J
D000368:Aged; D002869:Chromosome Aberrations; D003582:Cytogenetics / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D014407:Tumor Cells, Cultured