title: Molecular evolutionary analysis and mutational pattern of full-length genomes of hepatitis B virus isolated from Belgian patients with different clinical manifestations.
abstract: Molecular evolutionary patterns of 62 HBV full-length genomes obtained from Belgian patients were characterized. Phylogenetic analysis revealed diverse HBV subgenotypes including A2 and A6 (46.8%), D1-D4 (38.8%), E (9.7%), C1 (1.6%), and B2 (1.6%). The study population consisted of patients with different ethnic origin (Caucasian, Turkish, Asian, Arab, and African). One HBV D/C recombinant isolate was identified, which encoded subtype adw2. An HBV subgenotype D4 with an aberrant subtype ayw4 was detected. Although none of the genotypes was associated with a specific disease outcome, several nucleotide substitutions, deletions and insertions were observed within the HBV preS1/S and X genes, particularly among patients with active chronic hepatitis B infection and patients with cirrhosis. Within the immunological domain of the HBsAg gene, the most frequent substitutions were sT125M and sT118A. High rates of precore and basal core promoter mutations were detected in patients infected with genotype D of HBV. Almost half of the patients who received lamivudine therapy for at least 1 year had HBV variants associated with lamivudine drug resistance. In conclusion, the most common HBV genotypes in West Europe (A and D) also prevail in Belgium. The highest degree of genetic diversity was detected in HBV genotype D. In addition, this study reveals the circulation of exotic HBV genotypes B, C, and E in Belgium. J. Med. Virol. 82:379-389, 2010. (c) 2010 Wiley-Liss, Inc.
authors.affiliation: Laboratory of Clinical Virology, Rega Institute for Medical Research, Leuven, Belgium.
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Pourkarim
authors.forename: Samad
authors.initials: S
authors.lastname: Amini-Bavil-Olyaee
authors.forename: Jannick
authors.initials: J
authors.lastname: Verbeeck
authors.forename: Philippe
authors.initials: P
authors.lastname: Lemey
authors.forename: Mark
authors.initials: M
authors.lastname: Zeller
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
authors.forename: Piet
authors.initials: P
authors.lastname: Maes
authors.forename: Frederik
authors.initials: F
authors.lastname: Nevens
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D001530:Belgium; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D005060:Europe; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006509:Hepatitis B / Q000473:pathology* / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D017354:Point Mutation; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: D004279:DNA, Viral