Genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils.
Three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. The three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. Analysis of 16S rRNA gene sequence indicated that the isolates were related to members of the genera, Burkholderia, Arthrobacter, Pseudomonas, Variovorax, and Ensifer. The chromosomal DNA patterns of the isolates obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences were distinct from one another. Ten of the isolates had plasmids. All of the isolates and syntrophic pairs were able to degrade parathion-related compounds such as EPN, p-nitrophenol, fenitrothion, and methyl-parathion. When analyzed with PCR amplification and dot-blotting hybridization using various primers targeted for the organophosphorus pesticide hydrolase genes of previously-reported isolates, most of the isolates did not show positive signals, suggesting that their parathion hydrolase genes had no significant sequence homology with those of the previously-reported organophosphate pesticide-degrading isolates.
Choi
Min-Kyeong
MK
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
Kim
Kyung-Duk
KD
Ahn
Kyong-Mok
KM
Shin
Dong-Hyun
DH
Hwang
Jae-Hong
JH
Seong
Chi Nam
CN
Ka
Jong-Ok
JO
D001173:Arthrobacter / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D014644:Genetic Variation; D016946:Gram-Negative Aerobic Rods and Cocci / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006867:Hydrolases / Q000032:analysis / Q000235:genetics; D007306:Insecticides / Q000378:metabolism*; D012275:Oryza; D010278:Parathion / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D020579:Sinorhizobium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D010278:Parathion; D006867:Hydrolases