title: Illuminating plant biology: using fluorescent proteins for high-throughput analysis of protein localization and function in plants.
abstract: First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724, USA.
authors.forename: Stacy L
authors.initials: SL
authors.lastname: DeBlasio
authors.forename: Anne W
authors.initials: AW
authors.lastname: Sylvester
authors.forename: David
authors.initials: D
authors.lastname: Jackson
mesh_terms: D004742:Enhancer Elements, Genetic / Q000235:genetics; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D008164:Luminescent Proteins