[Immune protection of recombinant membrane protein against Taenia multiceps larvae in sheep].
OBJECTIVE
To investigate the immune protection of the recombinant membrane protein(45M) against larvae of Taenia multiceps in sheep.


METHODS
Twelve sheep were randomly divided into immune group and control group, and each sheep was immunized four times (three weeks interval) respectively with 50 microg recombinant 45M or GST emulsified with adjuvant. Serum samples were collected at pre-immunization and certain time after immunization, and were analyzed for IgG and IgM by ELISA. At the 105th day after the final inoculation, each sheep was orally challenged by 5000 eggs of T. multiceps. Two weeks after infection, the sheep were sacrificed, serum samples were collected, and number of cysts in brain tissue was counted. Activated oncospheres were cultured with 10% sera of immune group or control group, and observed by light microscopy.


RESULTS
The mean number of cysts was 1.5, and the average diameter of the cysts was 2.2 mm. Compared with control group, the reduction rate of cysts in immunized sheep was 68.9%. At the 72th hour after culture with serum of immunized sheep, about 90% oncospheres were killed by antiserum, and the oncospheres began to shrink or expand, and internal structure became fuzzy. ELISA showed that after final immunization (the 9th week), the levels of IgG in sera of immune group (2.32+/-0.76) were significantly higher than those of the control (0.70+/-0.42) (t=4.47, P<0.01). At the 24th week the levels of IgG and IgM in sera of immune group (1.53+/- 0.81, 0.90+/-0.26) were significantly higher than those of the control (0.64+/-0.43, 0.43+/-0.15) (P<0.01).


CONCLUSIONS
The recombinant 45M can induce certain humoral immune response.
Zhang
Zhuang-Zhi
ZZ
Institute of Veterinary Research, Xinjiang Academy of Animal Science, Urumqi, China. zzhuangzhi@yahoo.com.cn
Shi
Bao-Xin
BX
You
Hong
H
Zhao
Li
L
Tuerhong
Yimiti
Y
Wang
Jin-Cheng
JC
Zhang
Wen-Bao
WB
Cui
De-Sheng
DS
Aierken
Guo
Jin-Liang
JL
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000917:Antibody Formation; D003551:Cysticercosis / Q000276:immunology / Q000662:veterinary*; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000517:prevention & control; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D008565:Membrane Proteins; D011994:Recombinant Proteins