title: Free radical scavenging activity and antiproliferative potential of Polygonum cuspidatum root extracts.
abstract: Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, ethanol and ethyl acetate extracts of P. cuspidatum root were assayed for their 1,1-diphenyl-2-hydrazyl (DPPH) and hydroxyl free radical scavenging activities, total phenolics content, protective effect against DNA damage, and antiproliferative activity on human lung cancer cells. The ethanol and ethyl acetate (lipophilic phase) extracts of P. cuspidatum had significant scavenging effects on DPPH and hydroxyl radicals. Total phenolics content of ethanol and ethyl acetate (lipophilic phase) extracts of P. cuspidatum were 276.78 +/- 39.31 and 231.73 +/- 5.04 mg/ml, respectively; both extracts protected against hydroxyl radical-induced DNA strand scission. Furthermore, the extracts of P. cuspidatum induced apoptosis and inhibited cell growth in A549 and H1650 cell lines, suggesting that P. cuspidatum root extracts exhibit an antiproliferative effect on human lung cancer cells.
authors.affiliation: Department of Biochemical Science and Technology, National Chiayi University, 300 University Road, Chiayi, Taiwan, ROC.
authors.forename: Yun-Wei
authors.initials: YW
authors.lastname: Lin
authors.forename: Fu-Jung
authors.initials: FJ
authors.lastname: Yang
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Chen
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Lee
authors.forename: Ruey-Shyang
authors.initials: RS
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D045468:Fallopia japonica; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D014407:Tumor Cells, Cultured
chemical_list: D016166:Free Radical Scavengers; D006131:Growth Inhibitors; D010936:Plant Extracts