title: Biotic elicitation of isoflavone metabolism with plant growth promoting rhizobacteria in early stages of development in Glycine max var. Osumi.
abstract: Nine plant growth-promoting rhizobacteria from different backgrounds were assayed on Glycine max var. Osumi to evaluate their potential as biotic elicitors to increase isoflavone (IF) levels. Strains were inoculated on 2 day old pregerminated seeds. Six days after inoculation, the seedlings were harvested. Biometric parameters were registered, and IFs were determined. Although only one strain (N21.4) increased total IF contents and only one (M84) caused significant decreases in total IF, five different behaviors were detected when the daidzein and genistein families were analyzed separately. All strains triggered IF metabolism so further studies have to be developed since the different beneficial effects of IF through the diet may be due to the different IF profiles. These are encouraging results from two points of view: (1) N21.4 increases IF in seedlings, and (2) all other beneficial strains trigger IF metabolism differentially; hence, both facts could be used to prepare food supplements or as enriched standardized foods after full development of the biotechnological procedure.
authors.affiliation: Department of Biology, School of Farmacy, San Pablo CEU University, PO Box 67, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ramos-Solano
authors.forename: Elena
authors.initials: E
authors.lastname: Algar
authors.forename: Ana
authors.initials: A
authors.lastname: García-Villaraco
authors.forename: Jorge
authors.initials: J
authors.lastname: García-Cristóbal
authors.forename: J Antonio
authors.initials: JA
authors.lastname: Lucas García
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Gutierrez-Mañero
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena; D007529:Isoflavones / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology; D013025:Glycine max / Q000254:growth & development* / Q000378:metabolism* / Q000382:microbiology; D013559:Symbiosis
chemical_list: D007529:Isoflavones