[Gene identification of mumps wild virus isolated firstly in Jilin province].
OBJECTIVE
To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.


METHODS
Throat swabs were collected and to isolated mump virus with Vero/slam cells.Cytopathic effect was observed and determined mump virus type with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).


RESULTS
Of the total 15 throat swabs, different CPE were observed in 3 throat swabs but genotypes are same, they are F type, 3 throat swabs were not observed cytopathic effect but genotypes were F type.


CONCLUSIONS
There were different kinds of cytopathic effect in one mump outbreak although genotypes were same.
Wang
Shuang
S
Jilin Provincial Center for Disease Control and Prevention,Key Laboratory of Health Bureau ofJilin Province, Changchun 130062, Jilin, China.
Chang
Xin
X
Zhou
Jian-Hui
JH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000821:virology*; D009109:Mumps virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010614:Pharynx / Q000821:virology; D014709:Vero Cells; D055815:Young Adult