title: A dispensable role for P450(scc) in the overproduction of aldosterone in aldosterone-producing adenoma and idiopathic hyperaldosteronism in patients with primary aldosteronism.
abstract: Our previous study suggests that cytochrome P-450 carbon 17α-hydroxylase/17,20-lyase (P450(c17α)) correlated with the overproduction of aldosterone in aldosterone-producing adenoma (APA) and idiopathic hyperaldosteronism (IHA) in patients with primary aldosteronism. To further investigate if cytochrome P-450 cholesterol side-chain cleavage enzyme (P450(scc)) contributes to the overproduction of aldosterone in APA and IHA and if its mRNA expression differs in APA and IHA in patients with primary aldosteronism, we studied the expression of P450(scc) mRNA in APA and idiopathic hyperplastic nodules. Total RNA was extracted from APA of eight patients diagnosed as APA, idiopathic hyperplastic nodules of four patients diagnosed as IHA, seven normal adrenal glands and one normal muscle tissue. P450(scc) mRNA was examined by Northern blot analysis. No significant difference in P450(scc) mRNA was found among normal adrenal gland, APA or idiopathic hyperplastic nodules (P > 0.05). These results suggest that P450(scc) contributes little to the overproduction of aldosterone in APA and IHA and cannot be considered as a marker to differentiate between them in patients with primary aldosteronism.
authors.affiliation: Department of Internal Medicine, University of Missouri, Columbia, MO 65211, USA. fangy@health.missouri.edu
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Meifu
authors.initials: M
authors.lastname: Zang
authors.forename: Songsen
authors.initials: S
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Xu
authors.initials: X
authors.lastname: Di
authors.forename: Alicia
authors.initials: A
authors.lastname: Duren
mesh_terms: D018246:Adrenocortical Adenoma / Q000201:enzymology / Q000378:metabolism*; D000328:Adult; D000450:Aldosterone / Q000096:biosynthesis* / Q000378:metabolism; D015152:Blotting, Northern; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D000450:Aldosterone; D002786:Cholesterol Side-Chain Cleavage Enzyme