[Genetype characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008].
Genetic characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008 were analyzed. All samples were detected by RT-PCR using EV71-specific primers. The VP1s of EV71 strains were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with those of subgenotype A, B and C using DNASTAR, BioEdit and Mega 3.1 softwares. The VP1 nucleotide sequences of 17 strains of Shenzhen shared 95.3%-99.4% identities with cluster C4a, and one strain shared 93.0%-95.6% identities with cluster C4b. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was 81.8%-86.0%. Among 18 strains, the homogeneity of the VP1 nucleotide sequence was between 92.5%-100%. All EV71 strains circulating in Shenzhen were of subgenotype C4. The predominant strain of EV71 belonged to cluster C4a, also there was cluster C4b.
He
Ya-Qing
YQ
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. heyaqing1019@126.com
Qi
Rui
R
Yang
Hong
H
Xian
Hui-Xia
HX
Yao
Xiang-Jie
XJ
Yang
Fan
F
Zhang
Hai-Long
HL
Yang
Xiao-Ke
XK
Xu
Wen-Bo
WB
D002460:Cell Line; D002681:China; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins