Loading neurons with dextran-conjugated calcium indicators in intact nervous tissue.
Dextran-conjugated Ca(2+) indicators are retained well in neurons for many days following loading in intact or semi-intact brain tissue. Methods for loading neurons, as well as discussion of the unique properties of dextran-conjugated dyes which need to be considered for their use, are presented.
Delaney
Kerry R
KR
University of Victoria, Victoria, British Columbia, Canada.
D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D004396:Coloring Agents / Q000008:administration & dosage*; D003911:Dextrans / Q000008:administration & dosage*; D018274:Electroporation / Q000379:methods; D005453:Fluorescence; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*
D004396:Coloring Agents; D003911:Dextrans; D002118:Calcium