title: Histological evaluation of periodontal regeneration in autogenous tooth transplantation in the dog: a comparison between one and two-stage surgical techniques, a pilot study.
abstract: OBJECTIVE
  To compare the periodontal regeneration, associated with autogenous tooth transplantation in dogs, using either one- or two-stage surgical techniques.
  
  
  METHODS
  The study group consisted of three Beagles, older than 5 months, in which six incisors and six premolars were transplanted to mechanically prepared recipient sockets. One group was transplanted using a one-stage method to recipient beds prepared immediately before transplantation. The second group of teeth were transplanted using a two-stage method in which the recipient beds were prepared and left to heal for 7 days before transplantation. Clinical examinations were done every week and the animals were euthanized 9 weeks later. Subsequently, decalcified sections were prepared for routine and immunohistochemical histological evaluation. Periodontal healing was evaluated by undertaking histomorphometric analysis and analyzed using the Mann-Whitney test (P = 0.05).
  
  
  RESULTS
  All the transplanted teeth in both groups survived. No statistically significant difference was found in the complete healing between the treatment groups (P = 0.053). There was no difference between the occurrence of inflammatory root resorption (P = 0.135) and replacement root resorption (P = 0.081).
  
  
  CONCLUSIONS
  This study demonstrated that there was no difference between the two surgical techniques in terms of preventing root resorption in transplanted teeth.
authors.affiliation: Department of Dentistry, University School of Medicine, Coimbra, Portugal. m.mferreira@netcabo.pt
authors.forename: Ferreira Manuel
authors.initials: FM
authors.lastname: Marques
authors.forename: Botelho Maria
authors.initials: BM
authors.lastname: Filomena
authors.forename: Carvalho
authors.initials: C
authors.lastname: Lina
authors.forename: Oliveiros
authors.initials: O
authors.lastname: Barbara
authors.forename: Carrilho Eunice Virgínia
authors.initials: CE
authors.lastname: Palmeirão
mesh_terms: D000818:Animals; D001641:Bicuspid / Q000637:transplantation*; D001861:Bone Regeneration; D004285:Dogs; D007180:Incisor / Q000637:transplantation*; D008297:Male; D019647:Oral Surgical Procedures / Q000009:adverse effects / Q000379:methods*; D010519:Periodontium / Q000502:physiology*; D010865:Pilot Projects; D012391:Root Resorption / Q000209:etiology / Q000517:prevention & control*; D020390:Tooth Socket / Q000601:surgery