title: Steroids and triterpenes from the fruit bodies of Ganoderma lucidum and their anti-complement activity.
abstract: To determine the anti-complement activity of natural triterpenes, chromatographic separation of the EtOAc-soluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of three steroids and five triterpenoids. They were identified as ergosterol peroxide (1), ergosterol (2), genoderic acid Sz (3), stella sterol (4), ganoderic aic C1 (5), ganoderic acid A (6), methyl ganoderate A (7), and lucidenic acid A (8) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Compounds 2 and 3 showed potent anti-complement activity with IC50 values of 52.0 and 44.6 microM, respectively. Compound 1 exhibited significant inhibitory activity with an IC50 value of 126.8 microM, whereas compounds 4-8 were inactive. Our findings suggested that in addition to the ketone group at C-3, the delta7(8), delta9(11)-lanostadiene type triterpene also plays an important role in inhibiting the hemolytic activity of human serum against erythrocytes.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, Gyeongbuk 712-702, Korea.
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: MinKyun
authors.initials: M
authors.lastname: Na
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Jung
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Kim
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: IkSoo
authors.initials: I
authors.lastname: Lee
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000818:Animals; D003167:Complement Activation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D048690:Fruiting Bodies, Fungal; D006801:Humans; D020128:Inhibitory Concentration 50; D020940:Reishi / Q000737:chemistry*; D012756:Sheep; D013256:Steroids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D013256:Steroids; D014315:Triterpenes