title: Metabolomic screening applied to rice FOX Arabidopsis lines leads to the identification of a gene-changing nitrogen metabolism.
abstract: Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX Arabidopsis lines) using a gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candidate lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Arabidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.
authors.affiliation: RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan.
authors.forename: Doris
authors.initials: D
authors.lastname: Albinsky
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi
authors.forename: Naomi
authors.initials: N
authors.lastname: Hayashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukushima
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Takanari
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsui
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuroda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Horii
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsumoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hirochika
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002849:Chromatography, Gas; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins; D009584:Nitrogen