title: [Expression and purification of GST-HPV16E7 fusion protein].
abstract: OBJECTIVE
  To construct a recombinant prokaryotic expression vector efficiently expressing HPV16E7 and purify GST-HPV16E7 fusion protein.
  
  
  METHODS
  The HPV16E7 gene obtained from a local cervical cancer patient was cloned into vector pGEX-4T-1, to generate the recombinant named as pGEX-4T-1-HPV16E7. The recombinant plasmid was transformed into E. coli BL21. After inducing with IPTG the HPV16E7 fusion protein was analyzed by SDS-PAGE and Western blot. B-PER GST Fusion Protein Purification Kit was appkied to purify GST-HPV16E7 fusion protein.
  
  
  RESULTS
  The recombinant plasmid pGEX-4T-1-HPV16E7 was successfully constructed. Highly expressed and purified GST-HPV16E7 fusion protein was obtained. The specificity of fusion protein was verified by SDS-PAGE and Western blot.
  
  
  CONCLUSIONS
  GST-HPV16E7 fusion protein was successfully expressed and purified.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Xi
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D018307:Neoplasms, Squamous Cell / Q000473:pathology / Q000821:virology; D050725:Papillomavirus E7 Proteins / Q000096:biosynthesis* / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology
chemical_list: D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D005982:Glutathione Transferase