Gongronella sp induces overproduction of laccase in Panus rudis.
Laccase is usually produced via chemical induction and is also synthesized by hosts in interaction with the typical bio-control genus Trichoderma. In this study, we found that a newly isolated non-laccase-producing fungus, Gongronella sp. W5, could induce overproduction of laccase in Panus rudis. The enzyme activity, 148,200 U l(-1), was 25 times higher than the activity obtained from a chemical induction using copper/o -toluidine as inducers. A new laccase isozyme from the interaction of P. rudis and G. W5 was purified and characterized. A further test showed that some pH resistant metabolites secreted by G. W5 acted as signals to induce P. rudis laccase. Laccase is also highly expressed by Trametes sp. AH28-2 in interaction with Trichoderma sp. ZH1. However, no laccase activity was observed from the cross-over interactions of P. rudis -Trichoderma sp. ZH1 or Trametes sp. AH28-2-G. W5.
Wei
Fen
F
School of Life Sciences and Biotechnology Center, Anhui University, Hefei, China.
Hong
Yuzhi
Y
Liu
Juanjuan
J
Yuan
Jing
J
Fang
Wei
W
Peng
Hui
H
Xiao
Yazhong
Y
D018920:Coculture Techniques; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D042845:Laccase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D009090:Mucorales / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D020072:Polyporales / Q000201:enzymology*; D055454:Trametes / Q000378:metabolism; D014242:Trichoderma / Q000378:metabolism
D003470:Culture Media; D005656:Fungal Proteins; D007527:Isoenzymes; D042845:Laccase