title: Soluble, folded and active subtilisin in a protic ionic liquid.
abstract: The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.
authors.affiliation: CNAP, Department of Biology, University of York, York, United Kingdom YO10 5YW.
authors.forename: Francesco
authors.initials: F
authors.lastname: Falcioni
authors.forename: Hazel R
authors.initials: HR
authors.lastname: Housden
authors.forename: Zhenlian
authors.initials: Z
authors.lastname: Ling
authors.forename: Seishi
authors.initials: S
authors.lastname: Shimizu
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Walker
authors.forename: Neil C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D052578:Ionic Liquids / Q000737:chemistry*; D017510:Protein Folding; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012995:Solubility; D020860:Subtilisin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D052578:Ionic Liquids; D000644:Quaternary Ammonium Compounds; D002918:Chymotrypsin; D020860:Subtilisin