title: Plant antimicrobial peptides: an overview of SuperSAGE transcriptional profile and a functional review.
abstract: Defensin, thionin and lipid transfer protein (LTP) gene families, which antimicrobial activity has an attractive use in protein engineering and transgenic production of agronomical important plants, have been here functionally reviewed. Also, a transcriptional overview of a set of plant SuperSAGE libraries and analysis looking for 26 bp tags possibly annotated for those families is presented. Tags differentially expressed (p = 0.05) or constitutively transcribed were identified from leaves or roots SuperSAGE libraries from important Brazilian plant species [cowpea (Vigna unguiculata (L.) Walp.), soybean (Glycine max (L.) Merr.) and modern sugarcane hybrids (Saccharum spp.)] submitted to abiotic [salt (100 mM NaCl) or drought] or biotic stresses [fungus inoculation (Phakopsora pachyrhizi; Asiatic Soyben Rust phytopathogen)]. The diverse transcriptional patterns observed, probably related to the variable range of targets and functions involved, could be the first step to unravel the antimicrobial peptide world and the plant stress response relationship. Moreover, SuperSAGE opens the opportunity to find some SNPs or even rare transcript that could be important on plant stress resistance mechanisms. Putative defensin or LTP identified by SuperSAGE following a specific plant treatment or physiological condition could be useful for future use in genetic improvement of plants.
authors.affiliation: Department of Genetics, Federal University of Pernambuco, Recife, PE, Brazil. ederson.kido@gmail.com
authors.forename: E A
authors.initials: EA
authors.lastname: Kido
authors.forename: V
authors.initials: V
authors.lastname: Pandolfi
authors.forename: L M
authors.initials: LM
authors.lastname: Houllou-Kido
authors.forename: P P
authors.initials: PP
authors.lastname: Andrade
authors.forename: F C
authors.initials: FC
authors.lastname: Marcelino
authors.forename: A L
authors.initials: AL
authors.lastname: Nepomuceno
authors.forename: R V
authors.initials: RV
authors.lastname: Abdelnoor
authors.forename: W L
authors.initials: WL
authors.lastname: Burnquist
authors.forename: A M
authors.initials: AM
authors.lastname: Benko-Iseppon
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001938:Brazil; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics* / Q000276:immunology
chemical_list: D023181:Antimicrobial Cationic Peptides