title: [Distribution study of two constituents in rat after oral administration of Smilax china extract].
abstract: OBJECTIVE
  To investigate the distribution in rat after oral administration of Smilax china extract.
  
  
  METHODS
  Oxyresveratrol and resveratrol were quantitatively determined by HPLC/DAD method in rat tissues including heart, liver, spleen, lung and kidney after oral administration of S. china extract. The separation was performed on a Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water using linear gradient elution and UV detection at wavelength of 320 nm. The flow rate was 1.0 mL x min(-1) and the column was kept at 40 degrees C.
  
  
  RESULTS
  The HPLC/DAD method for the simultaneous determination of oxyresveratrol and resveratrol in rat tissues was developed.
  
  
  CONCLUSIONS
  The method has been successfully applied for the distribution study of two active constituents in rat through oral administration of S. china extract.
authors.affiliation: Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. huilianh@163.com
authors.forename: Huilian
authors.initials: H
authors.lastname: Huang
authors.forename: Guangtong
authors.initials: G
authors.lastname: Chen
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Lu
authors.forename: Jinqiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D000077185:Resveratrol