Comparison of two LED fluorescence microscopy build-on modules for acid-fast smear microscopy.
METHODS
National Reference Laboratory, Benin.


OBJECTIVE
To compare the performance of Fraen FluoLED and LW Lumin light-emitting diode (LED) fluorescence microscopy modules.


METHODS
Acid-fast bacilli (AFB) smears, routinely examined with a classical fluorescence microscope, were blindly re-read with both LED systems at 200x magnification. Smears with discordant results were rechecked on all systems at 200x, and 100 randomly chosen smears were read again at 400x. Confirmed presence of AFB with any system was accepted as a true positive.


RESULTS
A total of 1937 smears were examined by all systems. The Fraen and LW detected 895 (46.2%) and 817 (42.2%) positive and scanty positive smears. After rechecking 201 smears, 15 false-positive and 61 false-negative results were declared for Fraen, against 11 and 135 for LW. The systems had similar false-positive rates (1.7% for Fraen and 1.4% for LW), but differed significantly regarding detection of confirmed microscopy positives (93.5% and 85.6% respectively, P < 0.00001). A high correlation between both LED systems was found at 400x magnification.


CONCLUSIONS
The Fraen LED fluorescence microscopy module performed significantly better than the LW LED at the most efficient 200x magnification. It was also more appreciated by all users. The LW module may perform equally well at higher magnification.
Affolabi
D
D
Laboratoire de Référence des Mycobactéries, Cotonou, Benin.
Torrea
G
G
Odoun
M
M
Senou
N
N
Ali Ligali
M
M
Anagonou
S
S
Van Deun
A
A
D001431:Bacteriological Techniques / Q000379:methods; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D016037:Single-Blind Method; D013183:Sputum / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology