Improved reproducibility in preparing precision-cut liver tissue slices.
Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great variability in the results, we sought to compare the reproducibility of tissue slices generated using the newly developed Leica VT1200 S vibrating blade microtome with Vibrocheck (LV) and the Krumdieck tissue slicer (KD) which has been the standard apparatus for this application so far. Liver samples from five different species (human, pig, cattle, rat, mouse) were cut and the reproducibility of slice thickness was analyzed by cross sectioning the PCLS. The quality of the sliced tissue was determined via measurement of the ATP content. As a result, we found an improved accuracy and reproducibility of rat, mouse and human tissue slices using the new Leica vibrating blade microtome.
Zimmermann
Martina
M
Department of Gastroenterology and Hepatology, University Hospital Tuebingen, Otfried-Mueller-Str. 10, 72076, Tuebingen, Germany.
Lampe
Johanna
J
Lange
Sebastian
S
Smirnow
Irina
I
Königsrainer
Alfred
A
Hann-von-Weyhern
Claus
C
Fend
Falko
F
Gregor
Michael
M
Bitzer
Michael
M
Lauer
Ulrich M
UM