title: Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria.
abstract: BACKGROUND
  We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.
  
  
  METHODS
  Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.
  
  
  RESULTS
  By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.
  
  
  CONCLUSIONS
  This is the first study demonstrated the occurrence of SHV-12 in Nigeria.
authors.affiliation: Tibbi Biyoloji AD, KOU Tip Fakultesi, Izmit, Turkey. mkasap@vt.edu
authors.forename: Murat
authors.initials: M
authors.lastname: Kasap
authors.forename: Kayode
authors.initials: K
authors.lastname: Fashae
authors.forename: Sinem
authors.initials: S
authors.lastname: Torol
authors.forename: Fetiye
authors.initials: F
authors.lastname: Kolayli
authors.forename: Fatma
authors.initials: F
authors.lastname: Budak
authors.forename: Haluk
authors.initials: H
authors.lastname: Vahaboglu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial / Q000235:genetics; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009549:Nigeria; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases