title: Detection and quantification of tear phospholipids and cholesterol in contact lens deposits: the effect of contact lens material and lens care solution.
abstract: OBJECTIVE
  To examine the deposition of tear phospholipids and cholesterol onto worn contact lenses and the effect of lens material and lens care solution.
  
  
  METHODS
  Lipids were extracted from tears and worn contact lenses using 2:1 chloroform:methanol and the extract washed with aqueous ammonium acetate, before analysis by electrospray ionization tandem mass spectrometry (ESI-MS/MS).
  
  
  RESULTS
  Twenty-three molecular lipids from the sphingomyelin (SM) and phosphatidylcholine (PC) classes were detected in tears, with total concentrations of each class determined to be 5 +/- 1 pmol/microL ( approximately 3.8 microg/mL) and 6 +/- 1 pmol/microL ( approximately 4.6 microg/mL), respectively. The profile of individual phospholipids in both of these classes was shown to be similar in contact lens deposits. Deposition of representative polar and nonpolar lipids were shown to be significantly higher on senofilcon A contact lenses, with approximately 59 ng/lens SM, 195 ng/lens PC, and 9.9 microg/lens cholesterol detected, whereas balafilcon A lens extracts contained approximately 19 ng/lens SM, 19 ng/lens PC, and 3.9 microg/lens cholesterol. Extracts from lenses disinfected and cleaned with two lens care solutions showed no significant differences in total PC and SM concentrations; however, a greater proportion of PC than SM was observed, compared with that in tears.
  
  
  CONCLUSIONS
  Phospholipid deposits extracted from worn contact lenses show a molecular profile similar to that in tears. The concentration of representative polar and nonpolar lipids deposited onto contact lenses is significantly affected by lens composition. There is a differential efficacy in the removal of PC and SM with lens care solutions.
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, New South Wales, Australia.
authors.forename: Jennifer T
authors.initials: JT
authors.lastname: Saville
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Mark D P
authors.initials: MD
authors.lastname: Willcox
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Blanksby
authors.forename: Todd W
authors.initials: TW
authors.lastname: Mitchell
mesh_terms: D000328:Adult; D002784:Cholesterol / Q000032:analysis*; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D012828:Silicones / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013666:Tears / Q000737:chemistry*
chemical_list: D018639:Contact Lens Solutions; D020100:Hydrogels; D010743:Phospholipids; D012828:Silicones; C523195:balafilcon A; C523196:senofilcon A; D002784:Cholesterol