[Effect of wuhu decoction on surface co-stimulation molecule expression of peripheral dendritic cells in infants with asthma].
OBJECTIVE
To study the effect of Wuhu Decoction (WHD) on the expression of co-stimulation molecule of peripheral dendritic cells (DC), CD80, CD83 and CD86, in infants with asthma, in order to provide practical basis for further elucidate the action mechanism of WHD in preventing and treating infantile asthma.


METHODS
Sixty infants with asthma of Fei phlegm-heat accumulation syndrome type were randomized into the treatment group treated with WHD and the control group treated with Western medicine (fluticasone propionate oral taking or inhalation). And 10 healthy infants were set as normal control. With Thomas method adopted, the DC were isolated from peripheral blood of all infants subjected. The expressions of surface co-stimulation molecules of DC, CD80, CD83 and CD86, were detected by flow cytometry. Their changes before and after treatment in different groups were analyzed and compared.


RESULTS
Expressions of CD80 and CD86 of peripheral blood DC in asthmatic infants were remarkably higher than those in the normal control (P<0.01). In the treated group, CD80 expression lowered from 18.06 +/- 4.53 before treatment to 13.18 +/- 3.02 after treatment and CD86 expression lowered from 38.61 +/- 10.54 to 29.65 +/- 8.55; while in the control group, the two expressions were lowered from 18.40 +/- 3.86 to 15.34 +/- 3.90, and from 38.29 +/- 11.67 to 35.88 +/- 13.85 respectively, the lowering in both groups were statistically significant (P<0.01 and P<0.05), but it was more significant in the treated group (P<0.05). As for CD83, no significant difference existed between groups and no change was found in either group after treatment (P>0.05).


CONCLUSIONS
WHD can regulate the co-stimulation molecules of dendritic cells in asthma infants to reduce the expressions of CD80 and CD86.
Huang
Ting
T
First Affiliated Hospital, Hunan Traditional Chinese Medical University, Changsha, China.
Wang
Meng-Qing
MQ
Luo
Yin-He
YH
D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000097:blood* / Q000188:drug therapy*; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002675:Child, Preschool; D003713:Dendritic Cells / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007223:Infant; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008517:Phytotherapy; D000097802:CD83 Antigen
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D004365:Drugs, Chinese Herbal; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen