title: [Differential expression of microRNAs associated with estrogen receptor alpha and progesterone receptor in typeIand typeII endometrial adenocarcinomas.].
abstract: OBJECTIVE
  To identify differentially expression of microRNAs associated with expression of estrogen receptor alpha (ERalpha) and progesterone receptor (PR) between type I and type II endometrial adenocarcinoma.
  
  
  METHODS
  Two kinds of endometrial adenocarcinoma cell lines, Ishikawa and KLE, was transplanted into nude mice and biopsied to identify the expression of ERalpha, PR and p53, and test their response to estrogen and progesterone. Cultured the two cell lines under the estrogen-free and progesterone-free circumstance, total RNA was isolated to identify the differentially expressed microRNAs by microarray for prediction the microRNAs which target ESR1 and PGR by software miRANDA and TargetScan, and then was validated by real-time PCR in two cell lines cultured both in vivo and in vitro and ten specimens from patients.
  
  
  RESULTS
  Ishikawa cell line was confirmed from type I endometrial adenocarcinoma, KLE cell line was confirmed from type II endometrial adenocarcinoma. One hundred and twenty-six differentially expressed microRNAs between the two cell lines were identified by microRNA microarray, among of which may target ESR1 included hsa-miR-100, 99a, and may target PGR included hsa-miR-378, 768-3p. The differential expression of hsa-miR-100, 99a, 378, 768-3p identified by microarray between Ishikawa and KLE in vivo and in vitro was equal to that by real-time PCR, while Hsa-miR-100 was significantly down expressed in type I group specimens compared to type II group (P < 0.01).
  
  
  CONCLUSIONS
  Hsa-miR-100 is significantly down-expressed in type I endometrial adenocarcinoma compared to type II, which may be a great potential to target ESR1.
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Keng
authors.initials: K
authors.lastname: Shen
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Zeng
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Yang
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Cao
authors.forename: Quan-Cai
authors.initials: QC
authors.lastname: Cui
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000818:Animals; D016889:Endometrial Neoplasms; D047628:Estrogen Receptor alpha / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D035683:MicroRNAs; D011980:Receptors, Progesterone