Gonadotrophin-releasing hormone neurons in a photoperiodic songbird express fos and egr-1 protein after a single long day.
Birds use a variety of environmental cues, such as day length, temperature and social interactions, to time reproductive efforts. For most seasonally breeding birds, day length is the most important cue and takes precedence over all others. Experimental manipulation of day length has shown that, in a number of galliformes and passeriformes, exposure to a single long day induces a rise in plasma luteinising hormone (LH). The mechanisms underlying this response are only beginning to be understood. In Japanese quail and Zonotrichia sparrows, one long day causes striking up-regulation of the protein products of immediate early genes (IEGs) in the mediobasal hypothalamus, near gonadotrophin-releasing hormone (GnRH) axons and terminals. Photoperiodic induction of the same proteins in the GnRH somata themselves, however, has not been described in these species. In the present study, we used immunohistochemistry to assay the induction of two IEGs, Fos and Egr-1, in the GnRH somata of male and female white-throated sparrows (Zonotrichia albicollis) exposed to a single long day. We found that immunoreactivity for both proteins increased in a subset of the GnRH neurones of the septo-preoptic area by the morning after the long day. Photo-induced expression of Egr-1 or Fos protein in GnRH neurones was limited to a population of cells in the medial preoptic area. Males showed significantly greater induction of both proteins in this population of GnRH neurones than did females, which is consistent with the hypothesis that males may be more sensitive to photic cues. Overall, the results obtained suggest that photostimulation stimulates new protein synthesis in GnRH neurones on a relatively rapid time scale. Further research is required to determine whether the GnRH somata are themselves integrating photic cues, or whether they are responding rapidly to an increased demand for GnRH synthesis.
Saab
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Department of Psychology, Emory University, Atlanta, GA 30322, USA. dmaney@emory.edu
Lange
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Maney
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D000818:Animals; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D004958:Estradiol / Q000097:blood; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009474:Neurons / Q000378:metabolism*; D017440:Photoperiod; D011301:Preoptic Area / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012737:Sex Factors; D020308:Songbirds / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D015854:Up-Regulation
D051766:Early Growth Response Protein 1; D016760:Proto-Oncogene Proteins c-fos; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone