Peptide aptamers against titanium-based implants identified through phage display.
Commercially pure titanium (cp-Ti) is widely used in the field of long-term clinical oral implantology owing to its ability to allow close bone-implant apposition. The optimization of its function based on artificial proteins has become a key issue in the development of improved cp-Ti implants. Here, we set out to identify peptide aptamers with preferential adsorption towards titanium-based implants through the phage display methodology. Fifteen sequences were selected in the third round of biopanning. One sequence, ATWVSPY (named TBP1), had a 40% repetition rate and exhibited the strongest binding affinity to cp-Ti disks. Ten sequences were selected in the fourth round, among which the repetition rate is 80% for TBP1 and 20% for TBP2 (GVGLPHT). The peptide aptamers against cp-Ti disks can provide an alternative method of functional coating for biomaterial surfaces.
Liu
Yan
Y
Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Mao
Jing
J
Zhou
Bin
B
Wei
Wei
W
Gong
Shiqiang
S
D000327:Adsorption; D000595:Amino Acid Sequence; D052158:Aptamers, Peptide / Q000302:isolation & purification* / Q000378:metabolism* / Q000493:pharmacokinetics; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D008422:Materials Testing; D019151:Peptide Library; D019736:Prostheses and Implants; D011485:Protein Binding; D013379:Substrate Specificity; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
D052158:Aptamers, Peptide; D020099:Coated Materials, Biocompatible; D019151:Peptide Library; D014025:Titanium