title: Interactions of diorganolead(IV) with 3-(2-thienyl)-2-sulfanylpropenoic acid and/or thiamine: chemical and in vitro and in vivo toxicological results.
abstract: The reactions of PbR(2)(OAc)(2) (R = Me, Ph) with 3-(2-thienyl)-2-sulfanylpropenoic acid (H(2)tspa) in methanol or ethanol afforded complexes [PbR(2)(tspa)] that electrospray ionization-mass spectrometry (ESI-MS) and IR data suggest are polymeric. X-ray studies showed that [PbPh(2)(tspa)(dmso)] x dmso, crystallized from a solution of [PbPh(2)(tspa)] in dmso, is dimeric, and that [HQ](2)[PbPh(2)(tspa)(2)] (Q = diisopropylamine), obtained after removal of [PbPh(2)(tspa)] from a reaction including Q, contains the monomeric anion [PbPh(2)(tspa)(2)](2-). In the solid state the lead atoms are O,S-chelated by the tspa(2-) ligands in all these products, and in the latter two have distorted octahedral coordination environments. NMR data suggest that tspa(2-) remains coordinated to PbR(2)(2+) in solution in dmso. Neither thiamine nor thiamine diphosphate reacted with PbMe(2)(NO(3))(2) in D(2)O. Prior addition of H(2)tspa protected LLC-PK1 renal proximal tubule cells against PbMe(2)(NO(3))(2); thiamine had no statistically significant effect by itself, but greatly potentiated the action of H(2)tspa. Administration of either H(2)tspa or thiamine to male albino Sprague-Dawley rats dosed 30 min previously with PbMe(2)(NO(3))(2) was associated with reduced inhibition of delta-ALAD by the organolead compound, and with lower lead levels in kidney and brain, but joint administration of both H(2)tspa and thiamine only lowered lead concentration in the kidney.
authors.affiliation: Departamento de Química Inorgánica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain. sergio.casas@usc.es
authors.forename: José S
authors.initials: JS
authors.lastname: Casas
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Castaño
authors.forename: Agustín
authors.initials: A
authors.lastname: Sánchez
authors.forename: José
authors.initials: J
authors.lastname: Sordo
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Torres
authors.forename: María D
authors.initials: MD
authors.lastname: Couce
authors.forename: Angeles
authors.initials: A
authors.lastname: Gato
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Lorenzo
authors.forename: M Félix
authors.initials: MF
authors.lastname: Camiña
authors.forename: Eduardo E
authors.initials: EE
authors.lastname: Castellano
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D011756:Diphosphates / Q000737:chemistry; D007854:Lead / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000097:blood / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000097:blood; D011437:Propylamines / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013831:Thiamine / Q000737:chemistry*
chemical_list: C549497:3-(2-thienyl)-2-sulfanylpropenoic acid; D000179:Acrylates; D011756:Diphosphates; D009942:Organometallic Compounds; D011437:Propylamines; D013438:Sulfhydryl Compounds; D007854:Lead; C007442:diisopropylamine; D000623:Porphobilinogen Synthase; D013831:Thiamine