title: Molecular identification of the parasites causing cutaneous  leishmaniasis on the Caribbean coast of Colombia.
abstract: All clinical manifestations of leishmaniasis exist in Colombia, the cutaneous form being the most frequent in the department of Sucre, where the Leishmania species associated with cutaneous leishmaniasis (CL) is unknown. This study was carried out to determine which Leishmania species was responsible for CL in Sucre, based on amplification and sequencing of the Cyt b gene. Isolates of Leishmania were obtained after CL diagnosis of eight patients who received attention in several health care centers of the study area. The nucleotide sequences obtained from patients were compared to Leishmania reference strains and six of the isolates identified as Leishmania (Viannia) braziliensis, the remaining two being identified as Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis. This represents the first report of the presence of L. (V.) guyanensis on the Caribbean coast of Colombia.
authors.affiliation: Grupo de Investigaciones Biomédicas, Universidad de Sucre, Cra. 14 No. 16B-32, A.A. 406, Sincelejo, Colombia.
authors.forename: Lily Paola
authors.initials: LP
authors.lastname: Martínez
authors.forename: Juan Alberto
authors.initials: JA
authors.lastname: Rebollo
authors.forename: Arturo Luis
authors.initials: AL
authors.lastname: Luna
authors.forename: Suljey
authors.initials: S
authors.lastname: Cochero
authors.forename: Eduar Elías
authors.initials: EE
authors.lastname: Bejarano
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D003105:Colombia; D045303:Cytochromes b / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007892:Leishmania braziliensis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018334:Leishmania guyanensis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D045303:Cytochromes b