title: Heightened reactive oxygen species generation in the antimicrobial activity of a three component iodinated chitosan-silver nanoparticle composite.
abstract: Herein we report synergy in antimicrobial activity of a chitosan-silver nanoparticle (CS-Ag NP) composite in the presence of molecular iodine. Green fluorescent protein (GFP) expressing recombinant Escherichia coli bacteria have been used to test the efficacy and establish the mechanism of action. Experimental evidence indicate significantly high bactericidal activity of the nanocomposite in the presence of iodine than either due to the composite, chitosan, Ag NP or iodine only. Transmission electron microscopy measurements revealed attachment of bacteria to the composite. In addition, flow cytometry results supported definite occurrence of cell wall damage of the bacteria treated with the composite in the presence of iodine. Further, the nanocomposite and iodine combination was found to exert reactive oxygen species (ROS) generated oxidative stress in the cytoplasm of bacterial cells, leading to cell death. Elucidation of the mechanism of synergy due to three potential antibacterial components suggested that on the surface of Ag NPs molecular iodine possibly generated iodine atom thus contributing toward free radical induced oxidative stress, whereas chitosan and Ag NPs facilitated the process of cell killing and thus collectively enhanced the potency of antimicrobial effect at the lowest concentrations of individual components.
authors.affiliation: Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahati-39, Assam, India.
authors.forename: Madhuchanda
authors.initials: M
authors.lastname: Banerjee
authors.forename: Sadhucharan
authors.initials: S
authors.lastname: Mallick
authors.forename: Anumita
authors.initials: A
authors.lastname: Paul
authors.forename: Arun
authors.initials: A
authors.lastname: Chattopadhyay
authors.forename: Siddhartha Sankar
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007455:Iodine / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D017382:Reactive Oxygen Species; D012834:Silver / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D017382:Reactive Oxygen Species; D049452:Green Fluorescent Proteins; D012834:Silver; D048271:Chitosan; D007455:Iodine