title: Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice.
abstract: Synaptotagmin II (syt II) is the specific protein receptor of botulinum neurotoxin B (BoNT/B), and the luminal domain of syt II contains toxin-binding sites that have a high affinity for BoNT/B. However, it is not yet clear whether the luminal domain of syt II (syt II-LD) inhibits the toxicity of BoNT/B by interfering with the toxin-receptor interaction. In this study, we characterized the binding of the purified recombinant syt II-LD to BoNT and revealed that the recombinant syt II-LD in vivo could provide protection against BoNT/B intoxication in mice models, and the neutralization effect could be improved by using gangliosides.
authors.affiliation: State Key Laboratory of Pathogens and Biosecurity, Institute of Microbiology and Epidemiology, Fengtai District, Beijing, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Hou
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.forename: Shizhong
authors.initials: S
authors.lastname: Bao
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Le
authors.initials: L
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Tu
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D001688:Biological Products / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D001905:Botulinum Toxins / Q000037:antagonists & inhibitors*; D019274:Botulinum Toxins, Type A; D001906:Botulism / Q000517:prevention & control*; D005732:Gangliosides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D050861:Synaptotagmin II / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000992:Antitoxins; D001688:Biological Products; D005732:Gangliosides; D011994:Recombinant Proteins; C494262:SYT2 protein, human; D050861:Synaptotagmin II; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A