title: In vitro reconstruction of tetronate RK-682 biosynthesis.
abstract: The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein-bound substrates.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, UK. ys293@mole.bio.cam.ac.uk
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Sun
authors.forename: Frank
authors.initials: F
authors.lastname: Hahn
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Demydchuk
authors.forename: James
authors.initials: J
authors.lastname: Chettle
authors.forename: Manuela
authors.initials: M
authors.lastname: Tosin
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry; D016930:Saccharopolyspora / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; C094097:RK 682; D010749:Phosphoprotein Phosphatases