Transcriptional target-based expression cloning of immunoregulatory molecules.
Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.
Lamason
Rebecca L
RL
Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in Immunology, The Johns Hopkins University School of Medicine, BRB 607, Baltimore, MD 21205, USA.
Lew
Stefanie M
SM
Pomerantz
Joel L
JL
D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D017930:Genes, Reporter; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D016207:Cytokines; D014157:Transcription Factors