[Differential expression of microRNAs associated with estrogen receptor alpha and progesterone receptor in typeIand typeII endometrial adenocarcinomas.].
OBJECTIVE
To identify differentially expression of microRNAs associated with expression of estrogen receptor alpha (ERalpha) and progesterone receptor (PR) between type I and type II endometrial adenocarcinoma.


METHODS
Two kinds of endometrial adenocarcinoma cell lines, Ishikawa and KLE, was transplanted into nude mice and biopsied to identify the expression of ERalpha, PR and p53, and test their response to estrogen and progesterone. Cultured the two cell lines under the estrogen-free and progesterone-free circumstance, total RNA was isolated to identify the differentially expressed microRNAs by microarray for prediction the microRNAs which target ESR1 and PGR by software miRANDA and TargetScan, and then was validated by real-time PCR in two cell lines cultured both in vivo and in vitro and ten specimens from patients.


RESULTS
Ishikawa cell line was confirmed from type I endometrial adenocarcinoma, KLE cell line was confirmed from type II endometrial adenocarcinoma. One hundred and twenty-six differentially expressed microRNAs between the two cell lines were identified by microRNA microarray, among of which may target ESR1 included hsa-miR-100, 99a, and may target PGR included hsa-miR-378, 768-3p. The differential expression of hsa-miR-100, 99a, 378, 768-3p identified by microarray between Ishikawa and KLE in vivo and in vitro was equal to that by real-time PCR, while Hsa-miR-100 was significantly down expressed in type I group specimens compared to type II group (P < 0.01).


CONCLUSIONS
Hsa-miR-100 is significantly down-expressed in type I endometrial adenocarcinoma compared to type II, which may be a great potential to target ESR1.
Zhou
Jing
J
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Shen
Keng
K
Zeng
Jian-Fang
JF
Yang
Jia-Xin
JX
Cao
Dong-Yan
DY
Cui
Quan-Cai
QC
D000230:Adenocarcinoma / Q000235:genetics; D000818:Animals; D016889:Endometrial Neoplasms; D047628:Estrogen Receptor alpha / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics; D011980:Receptors, Progesterone / Q000378:metabolism
D047628:Estrogen Receptor alpha; D035683:MicroRNAs; D011980:Receptors, Progesterone