Tips and tricks for preparing lampbrush chromosome spreads from Xenopus tropicalis oocytes.
Due to their large size and fine organization, lampbrush chromosomes (LBCs) of amphibian oocytes have been for decades one of the favorite tools of biologists for the analysis of transcriptional and post-transcriptional processes at the cytological level. The emergence of the diploid Xenopus tropicalis amphibian as a model organism for vertebrate developmental genetics and the accumulation of sequence data made available by its recent genomic sequencing, strongly revive the interest of LBCs as a powerful tool to study genes expressed during oogenesis. We describe here a detailed protocol for preparing LBCs from X. tropicalis oocyte and give practical advice to encourage a large number of researchers to become familiar with these chromosomes.
Penrad-Mobayed
May
M
Institut Jacques Monod, UMR7592, CNRS and University Paris Diderot-Paris 7, Department of Molecular and Cellular Pathology, Paris, France. penrad@ijm.univ-paris-diderot.fr
Kanhoush
Rasha
R
Perrin
Caroline
C
D000465:Algorithms; D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure; D020732:Cytogenetic Analysis / Q000295:instrumentation / Q000379:methods*; D003582:Cytogenetics / Q000379:methods*; D004171:Diploidy; D005260:Female; D008540:Meiosis; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008957:Models, Genetic; D009865:Oocytes / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014981:Xenopus / Q000378:metabolism*