Effect of topical mitomycin-C on total collagen deposits on the submucosa of intact vocal folds in swine.
OBJECTIVE
To compare the effects of topical mitomycin-C at different concentrations on submucosal collagen deposition on the vocal folds of swine.


METHODS
The animals were divided into three groups according to the composition of the topical solution to be applied to the vocal folds: 0.9% saline solution (control group); 4 mg/ml mitomycin-C (group 1) and 8 mg/ml mitomycin-C (group 2). Thirty days after the application, all animals were sacrificed, their vocal folds were collected and stained by the picrosirius red technique, and submucosal collagen deposition areas were estimated by the Image Pro Plus 4.5 software. Mann-Whitney test was used to compare differences between parameters of each group.


RESULTS
The means of the areas of submucosal collagen deposits on vocal folds were 3110.44 square micrometers (microm(2)), 3115.98 microm(2) and 3105.78 microm(2) for groups control, 1 and 2, respectively. There were no statistical differences across the three groups (p>0.05).


CONCLUSIONS
Mitomycin-C topically applied to intact vocal folds of swine did not alter submucosal collagen deposition.
Pereira
Marcelo Charles
MC
Graduate Program in Clinics and Surgery, Universidade Federal do Paraná - UFPR.
Repka
Carlos Domingues
CD
Camargo
Paulo Antonio Monteiro
PA
Rispoli
Daniel Zeni
DZ
Campos
Antônio Carlos Ligocki
AC
Matias
Jorge Eduardo Fouto
JE
D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000187:drug effects* / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage* / Q000494:pharmacology; D009092:Mucous Membrane / Q000378:metabolism; D013552:Swine; D014827:Vocal Cords / Q000187:drug effects* / Q000378:metabolism*
D003432:Cross-Linking Reagents; D016685:Mitomycin; D003094:Collagen