[Analysis of 2,4-dibromophenol and 2,6-dibromophenol in phenolic-smelling flatfish].
A simple analytical method for dibromophenols (DBPs) in flatfiish was developed. 2,4-DBP and 2,6-DBP were extracted from a sample with acetone and n-hexane, cleaned up by treatment with conc. sulfuric acid and concentrated under a stream of nitrogen gas. The resulting extract was subjected to GC/MS. The recoveries of 2,4-DBP and 2,6-DBP were 89.4-96.5% and 81.4-86.2%, respectively, indicating that this method is useful for analysis of 2,4-DBP and 2,6-DBP. In the phenolic-smelling flatfish, 2,6-DBP was detected at the levels of 0.10 microg/g and 0.01 microg/g in skin and muscle, respectively, and 2,4-DBP was detected at the level of 0.02 microg/g in skin.
Tanaka
Yasuo
Y
Yokohama City Institute of Health, Takigashira, Isogo-ku, Yokohama, Japan.
Takahashi
Kyoko
K
Hosoi
Shiro
S
Hidaka
Toshio
T
Nakazawa
Hiroyuki
H
D000096:Acetone; D000818:Animals; D005396:Fish Products / Q000032:analysis*; D005412:Flatfishes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006586:Hexanes; D009812:Odorants / Q000032:analysis*; D010636:Phenols / Q000302:isolation & purification*; D012997:Solvents
D006586:Hexanes; D010636:Phenols; D012997:Solvents; D000096:Acetone; C038964:2,6-dibromophenol; C026385:n-hexane; C013930:2,4-dibromophenol