Photoassisted synthesis of luminescent mannose-Au nanodots for the detection of thyroglobulin in serum.
We have employed mannose-modified gold nanodots (Man-Au NDs) as a luminescence sensor for the detection of the thyroid-cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man-Au NDs are prepared through the reaction of 2.9 nm-diameter gold nanoparticles (Au NPs) with 11-mercapto-3,6,9-trioxaundecyl-alpha-D-mannopyranoside (Man-RSH) under the irradiation of a light-emitting diode (LED). We have found that the irradiation enhances the quantum yield (approximately 11%), alters the emission wavelength and lifetimes, and shortens the preparation time. A luminescence assay has been developed for Tg based on the competition between Tg and Man-Au NDs for the interaction with the concanavalin A (Con A). Because luminescence quenching of the Man-Au NDs by Con A is inhibited by Tg selectivity, we have obtained a highly sensitive and selective assay for Tg.
Huang
Chih-Ching
CC
Institute of Bioscience and Biotechnology, Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, 2, Beining Road, Keelung, 20224, Taiwan. huanging@ntou.edu.tw
Hung
Yu-Lun
YL
Shiang
Yen-Chun
YC
Lin
Tai-Yuan
TY
Lin
Yu-Shen
YS
Chen
Chao-Tsen
CT
Chang
Huan-Tsung
HT
D015374:Biosensing Techniques; D003208:Concanavalin A / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D008358:Mannose / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010777:Photochemistry; D013954:Thyroglobulin / Q000097:blood*
D005456:Fluorescent Dyes; D003208:Concanavalin A; D006046:Gold; D013954:Thyroglobulin; D008358:Mannose