title: Microspheres of mixed proteins.
abstract: This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co-workers who have developed a method by which high-intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water-insoluble liquids.1 By using high-intensity ultrasound, we have synthesized microspheres made of a few different proteins. The three proteins used in the current experiments are bovine serum albumin (BSA), green fluorescent protein (GFP), and cyan fluorescent protein-glucose binding protein-yellow fluorescent fused protein (CFP-GBP-YFP). The two synthesized microspheres made of mixed proteins are BSA-GFP and BSA-(CFP-GBP-YFP). This paper presents the characterization of the sonochemically produced microspheres of mixed proteins. It also provides an estimate of the efficiency of the sonochemical process in converting the native proteins to microspheres.
authors.affiliation: Department of Chemistry and Kanbar Laboratory for Nanomaterials, Bar-Ilan University Center for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel. gedanken@mail.biu.ac.il
authors.forename: Ulyana
authors.initials: U
authors.lastname: Angel Shimanovich
authors.forename: Devorah
authors.initials: D
authors.lastname: Matas
authors.forename: Shulamit
authors.initials: S
authors.lastname: Michaeli
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
authors.forename: Aharon
authors.initials: A
authors.lastname: Gedanken
mesh_terms: D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008823:Micelles; D008856:Microscopy, Fluorescence; D008863:Microspheres; D015394:Molecular Structure; D010316:Particle Size; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D029968:Escherichia coli Proteins; D008823:Micelles; D011506:Proteins; D012709:Serum Albumin; D049452:Green Fluorescent Proteins; D012710:Serum Albumin, Bovine