[Histone lysine methyltransferase Setd7 enhances Ngn 1 gene expression].
OBJECTIVE
To construct the eukaryotic expression plasmid of mouse histone lysine methyltransferase Setd7 and detect its effect on neuron development.


METHODS
The clone of mouse Setd7 was obtained and inserted into the eukaryotic expression vector pCMV-3tag-6-Flag. The plasmid was transfected into HEK 293T and identified by Western blot. Real-time PCR was used to detect the effect of Setd7 on the neuron differentiation marker gene Ngn 1 mRNA expression. Dual luciferase reporter system was used to detect the effect of Setd7 on Ngn 1 mRNA expression. Real-time PCR was used to detect the effect of Setd 7 siRNA plasmid on Ngn 1 mRNA expression.


RESULTS
An eukaryotic expression plasmid of Setd 7 was successfully constructed. Setd7 induced Ngn 1 mRNA expression and increased Ngn 1 promoter activity. Also, the knockdown of Setd 7 inhibited Ngn 1 mRNA expression.


CONCLUSIONS
Histone lysine methyltransferase Setd7 can enhance neuron differentiation marker gene Ngn 1 transcription.
Dai
Jin-Po
JP
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing 100005, China.
Zhang
Ye
Y
Shen
Yu-Fei
YF
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D057809:HEK293 Cells; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D011495:Histone-Lysine N-Methyltransferase; D009419:Nerve Tissue Proteins; D011496:Protein Methyltransferases; D012333:RNA, Messenger; C105389:NEUROG1 protein, human; C562044:Setd7 protein, mouse