title: Lunasin peptide purified from Solanum nigrum L. protects DNA from oxidative damage by suppressing the generation of hydroxyl radical via blocking fenton reaction.
abstract: Oxidative DNA damage is the most critical factor implicated in carcinogenesis and other disorders. However, the protective effects of lunasin against oxidative DNA damage have not yet reported. In this study, we report here the protective effect of lunasin purified from Solanum nigrum L. against oxidative DNA. Lunasin protected DNA from the oxidative damage induced by Fe(2+) ion and hydroxyl radical. To better understand the mechanism for the protective effect of lunasin against DNA damage, the abilities to chelate Fe(2+), scavenge the generated hydroxyl radical and block the generation of hydroxyl radical were evaluated. Although it did not scavenge generated hydroxyl radical, lunasin blocked the generation of hydroxyl radical by chelating Fe(2+) ion. We conclude that lunasin protects DNA from oxidation by blocking fenton reaction between Fe(2+) and H(2)O(2) by chelating Fe(2+) and that consumption of lunasin may play an important role in the chemoprevention for the oxidative carcinogenesis.
authors.affiliation: School of Bioresources Science, Andong National University, Andong, Republic of Korea.
authors.forename: Jin Boo
authors.initials: JB
authors.lastname: Jeong
authors.forename: Ben O
authors.initials: BO
authors.lastname: De Lumen
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000378:metabolism; D007501:Iron / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D032324:Solanum nigrum / Q000737:chemistry*
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; C045076:Fenton's reagent; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D033862:Iron-Binding Proteins; D010936:Plant Extracts; D010940:Plant Proteins; D017665:Hydroxyl Radical; D004247:DNA; D006861:Hydrogen Peroxide; D007501:Iron