title: Overexpression of the fragile histidine triad (FHIT) gene in inflammatory bowel disease.
abstract: OBJECTIVE
  FHIT gene encodes human diadenosine triphosphate hydrolase involved in the regulation of cell cycle and nucleotide metabolism and is a candidate tumor suppressor gene.
  
  
  OBJECTIVE
  To investigate expression of FHIT gene at the mRNA and protein levels in sporadic inflammatory bowel disease (IBD).
  
  
  METHODS
  FHIT mRNA was quantified by the validated real-time PCR (QPCR) and FHIT protein was detected by immunohistochemistry (IHC) in mucosal biopsies of 139 ulcerative colitis (UC), 19 Crohn's disease (CD) and 37 control patients.
  
  
  RESULTS
  Significant FHIT gene overexpression was found in 78% of active UC but not in CD. IHC showed comparable results to QPCR.
  
  
  CONCLUSIONS
  The local up-regulation of FHIT gene and protein expression in active UC may represent an adequate response against inflammatory challenge of epithelial cell homeostasis and protect against DNA damage and cell cycle disturbances.
authors.affiliation: Department of Histology, Medical University of Gdansk, Gdansk, Poland. pwierzb@amg.gda.pl
authors.forename: P M
authors.initials: PM
authors.lastname: Wierzbicki
authors.forename: K
authors.initials: K
authors.lastname: Adrych
authors.forename: D
authors.initials: D
authors.lastname: Kartanowicz
authors.forename: J
authors.initials: J
authors.lastname: Wypych
authors.forename: M
authors.initials: M
authors.lastname: Stanislawowski
authors.forename: M
authors.initials: M
authors.lastname: Zwolinska-Wcislo
authors.forename: K
authors.initials: K
authors.lastname: Celinski
authors.forename: D
authors.initials: D
authors.lastname: Skrodzka
authors.forename: J
authors.initials: J
authors.lastname: Godlewski
authors.forename: B
authors.initials: B
authors.lastname: Korybalski
authors.forename: M
authors.initials: M
authors.lastname: Smoczynski
authors.forename: Z
authors.initials: Z
authors.lastname: Kmiec
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017931:DNA Primers; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011044:Poland; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D017931:DNA Primers; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase