Identification and quantitation of zolpidem in biological matrices using gas chromatography-mass spectrometry (GC-MS).
Zolpidem is a short-acting nonbenzodiazepine hypnotic used for the treatment of insomnia. It works quickly and has a short half-life of 2-3 h. Significant side effects are reported including dizziness, amnesia, and even hallucinations. In this method, zolpidem is extracted from blood or other biological fluids/tissues using solid phase extraction technology for analysis. The extract is then assayed using selected ion monitoring gas chromatography/mass spectrometry for absolute confirmation of the compound. The quantitation of this drug is made from standard curves constructed using selected ion monitoring and peak-area ratios of analyte vs. internal standard of quantifying ions.
Rohrig
Timothy P
TP
Regional Forensic Science Center, Wichita, KS, USA.
Harryman
Lydia A
LA
Norton
Melissa C
MC
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011725:Pyridines / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D000077334:Zolpidem
D011725:Pyridines; D000077334:Zolpidem