title: [Cytophotometric expression of the tumor markers CD-34 and factor VIII in colorectal cancer].
abstract: OBJECTIVE
  In colorectal cancer, to describe the cytophotometric expression of the CD-34 and Factor VIII; to evaluate the degree of correlation between them; and to compare the CD-34 and Factor VIII expressions in relationship to label index and optical density.
  
  
  METHODS
  Seventeen cases of colorectal adenocarcinoma recovered from paraffin-embedded archival tissue and confirmed by hematoxilin-eosin staining, were submitted to streptavidin-biotin-peroxidase immunohistochemical method. In this process was used the tumor markers CD-34 and Factor VIII. The obtained slides were analysed using the SAMBA 4000 system with Immuno software. The results were evaluated into two parameters: label index and optical density, and expressed by averages, medians, minimum values, maximum values, and standard deviation values. The normality condition of the quantitative variables was investigated by using the Shapiro-Wilks test. In order to evaluate the degree of association between the expressions of the markers, Pearson's Correlation Coefficient or Spearman's Correlation Coefficient were applied. To evaluate the comparison degree of the markers expression, Student's t test or Wilcoxon's no parametric test were used.
  
  
  RESULTS
  For the CD-34 there was no data normality for the label index and there was normality in the optical density. For the Factor VIII, there was data normality for the label index and for the optical density.
  
  
  CONCLUSIONS
  When the expressions of CD-34 and Factor VIII markers were correlated, there was no difference between them in relationship to the average label index and average optical density. When the expressions of the CD-34 and Factor VIII were compared, there was no correlation between the two variables.
authors.affiliation: Programa de Pós-graduação, Faculdade Evangélica do Paraná, PR, BR. ipem@evangelico.org.br
authors.forename: Cacilda Joyce Ferreira da Silva
authors.initials: CJ
authors.lastname: Garcia
authors.forename: Ronaldo M
authors.initials: RM
authors.lastname: Cuenca
authors.forename: Fabio Roberto
authors.initials: FR
authors.lastname: Bora
authors.forename: Jurandir Marcondes
authors.initials: JM
authors.lastname: Ribas-Filho
authors.forename: Nicolau Gregori
authors.initials: NG
authors.lastname: Czeczko
authors.forename: Carmen Austrália Paredes Marcondes
authors.initials: CA
authors.lastname: Ribas
authors.forename: Jorge
authors.initials: J
authors.lastname: Wagenführ
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism*; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000378:metabolism*; D003592:Cytophotometry; D005169:Factor VIII / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D005169:Factor VIII