title: A porous scaffold for bone tissue engineering/45S5 Bioglass derived porous scaffolds for co-culturing osteoblasts and endothelial cells.
abstract: One of the major factors in the therapeutic success of bone tissue engineered scaffolds is the ability of the construct to vascularise post implantation. One of the approaches for improving vascularisation within scaffolds has been to co-culture human umbilical vein endothelial cells (HUVECS) with human osteoblasts (HOBS), which may then promote vascularisation and facilitate tissue regeneration. However, in order to mimic a natural physiological niche it is vital that the scaffold is able to support and promote the proliferation of both cell types and thus become a viable tissue engineered construct. In this study we report the development of a porous bioactive glass-ceramic construct and examine the interaction with human umbilical vein endothelial cells (HUVEC's) and human osteoblast-like cell both in mono and co-culture. The study clearly demonstrated that the scaffolds were able to support both endothelial and human osteoblast cell proliferation both in mono and co-culture. A comparison of the proliferation response of HUVEC and HOB in mono-culture on the test scaffolds and the commercial porous hydroxyapatite was assessed over a 28 day period (4, 7, 14, 21 and 28 days), using alamar Blue assay. Proliferation of HOB cells seeded in the scaffolds was consistently shown to be above those observed on commercial HA scaffolds.
authors.affiliation: Department of Biomaterials, Biomimetics and Biophotonics, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Hospital, London Bridge, SE1 9RT, London, UK. sanjukta.deb@kcl.ac.uk
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Deb
authors.forename: Ramin
authors.initials: R
authors.lastname: Mandegaran
authors.forename: Lucy
authors.initials: L
authors.lastname: Di Silvio
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D018920:Coculture Techniques / Q000379:methods*; D004396:Coloring Agents / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010006:Osteoblasts / Q000166:cytology*; D010078:Oxazines / Q000494:pharmacology; D016062:Porosity; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D004396:Coloring Agents; D010078:Oxazines; D013778:Tetrazolium Salts; D013844:Thiazoles; D014966:Xanthenes; C425330:bioactive glass 45S5; C005843:resazurin; C022616:thiazolyl blue