title: Platform for in situ real-time measurement of protein-induced conformational changes of DNA.
abstract: A platform for in situ and real-time measurement of protein-induced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia coli integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay.
authors.affiliation: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
authors.forename: Philipp S
authors.initials: PS
authors.lastname: Spuhler
authors.forename: Jelena
authors.initials: J
authors.lastname: Knezevic
authors.forename: Ayça
authors.initials: A
authors.lastname: Yalçin
authors.forename: Qiuye
authors.initials: Q
authors.lastname: Bao
authors.forename: Erika
authors.initials: E
authors.lastname: Pringsheim
authors.forename: Peter
authors.initials: P
authors.lastname: Dröge
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rant
authors.forename: M Selim
authors.initials: MS
authors.lastname: Unlü
mesh_terms: D015342:DNA Probes / Q000032:analysis* / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006046:Gold