[Screening, identification and bioinformatic analysis of Taenia solium cysticercus].
OBJECTIVE
To screen and identify specific antigens of Taenia solium cysticercus, and predict the function of target proteins using bioinformatics method.


METHODS
Patients infected with Taenia solium were dewormed by decoction arecae and pumpkin seeds to collect worms, and eggs were then prepared. Six three-way crossed hybrid pigs were randomly divided into experimental group and control group, and each experimental pig was infected with 80,000 T. solium eggs. Serum samples were collected at 40 days after infection. The total protein of T. solium cysticercus was separated by two-dimensional electrophoresis, and Western blotting was performed to find out distinct antigens. Proteins from the two groups were identified by ESI-Trap MS. Query in NCBI database was made to confirm function of the proteins.


RESULTS
207+/-9 spots were detected through Coomassie brilliant blue-stained gels with Mr 14,400-94,000 and pI 3.0-10.0. Western blotting showed 7 specific antigen spots with pool sera of infected pigs. Four of the 7 antigens with known functions were respectively ascribed to cytoskeletal actin-2 (adult-specific), tropomyosin (cysticercus-specific), AF239799-1 annexin (cysticercus-specific) and actin-1 (cysticercus-specific).


CONCLUSIONS
Three specific antigens of Taenia solium cysticercus have been identified.
Fang
Wen
W
Department of Clinical Laboratory Sciences, Guiyang Medical College, Guiyang, China.
Bao
Huai-En
HE
Xiao
Liang-Liang
LL
Mu
Rong
R
D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D019295:Computational Biology; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology; D003552:Cysticercus / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology; D041201:Taenia solium / Q000276:immunology*
D000947:Antigens, Helminth