Microbacterium radiodurans sp. nov., a UV radiation-resistant bacterium isolated from soil.
Strain GIMN 1.002(T), a UV radiation-tolerant bacterium, was isolated from the upper sand layers of the Gobi desert, Xinjiang, China and characterized in order to determine its taxonomic position. Cells were Gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16S rRNA gene sequence analysis revealed that strain GIMN 1.002(T) belonged to the genus Microbacterium and was closely related to Microbacterium arborescens DSM 20754(T) (98.8 % 16S rRNA gene sequence similarity) and Microbacterium imperiale DSM 20530(T) (98.7 %). However, strain GIMN 1.002(T) had low DNA-DNA relatedness with M. arborescens DSM 20754(T) (17.1 %) and M. imperiale DSM 20530(T) (12.89 %). Strain GIMN 1.002(T) possessed chemotaxonomic markers that were consistent with its classification in the genus Microbacterium, i.e. MK-11, MK-12 and MK-10 as major menaquinones and anteiso-C(15 : 0) (38.67 %), iso-C(16 : 0) (18.16 %) and iso-C(15 : 0) (17.46 %) as predominant cellular fatty acids. The DNA G+C content was 67.74 mol%. The cell-wall sugar was rhamnose. On the basis of the data from this study, strain GIMN 1.002(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium radiodurans sp. nov. is proposed. The type strain is GIMN 1.002(T) (=CCTCC M208212(T) =NRRL B-24799(T)).
Zhang
Wei
W
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
Zhu
Hong-Hui
HH
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, PR China.
Yuan
Menglong
M
College of Life Science, Beijing Normal University, Beijing 100875, PR China.|Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
Yao
Qing
Q
South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Tang
Ran
R
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
Lin
Min
M
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
Yang
Song-Zhen
SZ
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, PR China.
Li
Zhi-Kun
ZK
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
Chen
Ming
M
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S