Multiplex PCR assay to facilitate identification of the recently described Staphylococcal cassette chromosome mec type VIII.
We recently described a novel staphylococcal cassette chromosome mec (SCCmec) element, designated type VIII. We report here a multiplex PCR assay, capable of identifying SCCmec type VIII. This assay will facilitate identification of this new SCCmec type and provides a useful tool for clinical and epidemiologic studies in this regard.
McClure
Jo-Ann
JA
Centre for Antimicrobial Resistance, Alberta Health Services/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada.
Conly
John M
JM
Elsayed
Sameer
S
Zhang
Kunyan
K
D015373:Bacterial Typing Techniques; D001681:Biological Assay; D002876:Chromosomes, Bacterial / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*