title: [Infiltration of CD(4)(+)CD(25)(+) T cells into the allergic asthmatic airways caused by house dust mite antigen administration.].
abstract: OBJECTIVE
  To investigate whether house dust mite (HDM) could induce CD(4)(+) CD(25)(+) T cells infiltration into asthmatic airways in patients vivo.
  
  
  METHODS
  Ten subjects with asthma underwent initial bronchoscopy during which normal saline and HDM were administered to two sublobar segments separately. The second bronchoscopy were carried out and bronchoal lavage fluid from HDM-challenged sites and saline-challenged sites were separately taken 24 h later. The differential cell counts were determined, and the absolute number of each type was calculated. At the same time, CD(3)(+), CD(4)(+) and CD(4)(+) CD(25)(+) T cells were determined by flow-cytometric analysis. We compared cellular counts in airways without and after topical instillation of HDM.
  
  
  RESULTS
  Eosinophile granulocyte cells of broncho-alveolar fluid in the HDM-challenged sites (1.4 +/- 0.1) x 10(6)/ml are more than it in control sites (0.3 +/- 0.1) x 10(6)/ml, P < 0.003. Lymphocyte cells of BALF in the HDM-challenged sites (2.2 +/- 0.3) x 10(6)/ml are more than it in control sites (0.3 +/- 0.1) x 10(6)/ml, P < 0.001; CD(4)(+) CD(25)(+)T cells of BALF in the HDM-challenged sites (784.0 +/- 281.3) cell/microl are more than it in control sites (7.7 +/- 3.6) cell/microl, P < 0.001.
  
  
  CONCLUSIONS
  Our findings suggest that HDM is capable of inducing CD(4)(+) CD(25)(+) T cells recruitment into non-acute mild allergic asthmatic airways.
authors.affiliation: Institute of Respiratory Medicine of Guangxi Medical University, Nanning, Guangxi 530021, China. Email: chenyq163@tom.com.
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Chen
authors.forename: Hong-Dong
authors.initials: HD
authors.lastname: Huang
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wen
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Zou
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Deng
authors.forename: Huan-Zhong
authors.initials: HZ
authors.lastname: Shi
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma; D001992:Bronchoalveolar Lavage Fluid; D004804:Eosinophils; D006801:Humans; D039981:Pyroglyphidae; D013601:T-Lymphocytes
chemical_list: D039741:Antigens, Dermatophagoides