title: [Establishment of transgenic receptor system and aFGF transformation in astragalus].
abstract: OBJECTIVE
  To establish a high-frequency regeneration system of Astragalus and an aFGF transformation system.
  
  
  METHODS
  Cotyledon node of the Astragalus explants was used for organogenesis to establish a high-frequency regeneration system. GV3101 was used to transform cotyledon node, and aFGF gene was introduced into Astragalus, renewable strain was detected by PCR.
  
  
  RESULTS
  All cotyledon node was explants, adventitious buds were induced in the medium of MS +2.0 mg x L(-1) BA +0.5 mg x L(-1) IBA, the root was taken in the medium of 1/2MS +5.0 mg x L(-1) NAA to give a high frequency regeneration system. All cotyledon node was precultured in medium for 3 days and infected with Agrobacterium (A600 0.3) for 10 min and then cocultured for 2 days. The aFGF gene was confirmed to transform into genome of Astragalus.
  
  
  CONCLUSIONS
  A high-frequency regeneration system of Astragalus and an aFGF transformation system were established.
authors.affiliation: College of Life Science, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Dachao
authors.initials: D
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Shicui
authors.initials: S
authors.lastname: Jiang
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Han
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhang
mesh_terms: D027883:Astragalus Plant / Q000235:genetics* / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D016220:Fibroblast Growth Factor 1