title: Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
abstract: During the postpartum period there is a high incidence of mastitis in dairy cows. The reason for this increased risk of mastitis still remains unclear. Since leukocytes in colostrum have an important role in preventing the onset of mastitis, we investigated the leukocyte populations, which express CD4, CD8, CD14, CD21 or WC1, in colostrum as well as in blood obtained from 14 Holstein cows. Eight cows developed mastitis within a week after calving and the other 6 remained healthy. The percentage of CD14+ cells in colostrum was significantly lower in mastitic cows than in healthy cows. There were no significant differences in other marker positive cells either in the colostrum or in the blood. The CD14+ cells in colostrum play an important role of defense against invading microorganisms in the mammary glands. Our results suggested that the lower percentage of CD14+ cells in colostrum might predict the incidence of mastitis in the following period.
authors.affiliation: National Institute of Animal Health, 3-1-5 Kan-nondai, Tsukuba Ibaraki, 305-0856, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ozawa
authors.forename: Shiro
authors.initials: S
authors.lastname: Kushibiki
authors.forename: Madoka
authors.initials: M
authors.lastname: Sudo
authors.forename: Kouhei
authors.initials: K
authors.lastname: Kitazaki
authors.forename: Noriaki
authors.initials: N
authors.lastname: Abe
authors.forename: Takahashi
authors.initials: T
authors.lastname: Hideyuki
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D001806:Blood Urea Nitrogen; D002417:Cattle; D002784:Cholesterol / Q000097:blood; D003126:Colostrum / Q000276:immunology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007657:Ketone Bodies / Q000097:blood; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology*; D008414:Mastitis, Bovine / Q000097:blood / Q000276:immunology*; D008892:Milk / Q000276:immunology; D049590:Postpartum Period / Q000097:blood / Q000276:immunology
chemical_list: D001798:Blood Proteins; D005230:Fatty Acids, Nonesterified; D007657:Ketone Bodies; D018950:Lipopolysaccharide Receptors; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D020155:3-Hydroxybutyric Acid