Comparative analysis of expressed sequence tags from the white-rot fungi (Phanerochaete chrysosporium).
Comprehensive analysis of the transcriptome of the P. chrysosporium is a useful approach to improve our understanding of its special and unique enzyme system and fungal evolution in molecular and industrial aspects. In order to unveil the functional diversity of this white-rot fungus in gene level and the expression patterns of its genes, in this study we carried out sequencing and annotation of 4,917 P. chrysosporium expressed sequence tags (ESTs). Through our bioinformatic ESTs analysis, we elucidated that 1,751 genes were derived from the present dataset of 4,917 ESTs, based on clustering and comparative genomic analyses of the ESTs. Of the 1,751 unique ESTs, 1,006 (57.5%) had homologues and orthologues in similarity searches. Our P. chrysosporium ESTs showed many genes for encoding 23 secreted proteins, many proteins for the degradation of cellulose and hemicelluloses, and heat shock proteins for stress resistance, which explain the reason why P. chrysosporium is very important and unique white-rot fungus in dealing with contaminated resources and in degrading lignin and in applying this organism to several industrial aspects.In addition, comparative analysis has shed the fresh light on the mystery about how its unique enzyme system and stress resistance have been evolved differently from its closest relatives.
Kim
Dae-Won
DW
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Kim
Aeri
A
Kim
Ryong Nam
RN
Nam
Seong-Hyeuk
SH
Kang
Aram
A
Chung
Wan-Tae
WT
Choi
Sang-Haeng
SH
Park
Hong-Seog
HS
D002874:Chromosome Mapping; D019295:Computational Biology; D030541:Databases, Genetic; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008031:Lignin / Q000378:metabolism; D009492:Neurospora crassa / Q000235:genetics; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics
D005656:Fungal Proteins; D012333:RNA, Messenger; D008031:Lignin