Whole genomic expression analysis of octachlorostyrene-induced chronic toxicity in Caenorhabditis elegans.
In recent years, microarray technology has enabled the investigation of possible mechanisms the expression of genes related to toxic compounds. We used a C. elegans whole genome microarray to observe and evaluate the chronic toxicity of the free-living nematode Caenorhabditis elegans (C. elegans) after exposure to octachlorostyrene, (OCS), a by-product in the manufacture of many chlorinated hydrocarbons. In this study, we examined sublethal toxicity, egg hatching, and movement of octachlorostyrene over three generations using a nematode growth medium (NGM) agar plate. In the third generation, OCS affected the fecundity rate of C. elegans. Specifically, the number of worm and eggs decreased significantly to about 50% of control (p < 0.05). In microarray experiments, total RNA was isolated at 0, 2 and 3 generations following treatment of OCS, and hybridized to the microarray containing about 22,000 C. elegans genes. Dye swaps were performed. After data analysis, we identified a total of 1,294 genes that were differentially expressed through generations.
Kim
Seung Jun
SJ
Department of Biochemistry, Hanyang University & GenoCheck Co. Ltd., Ansan 426-791, Korea.
Choung
Se Young
SY
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D005298:Fertility / Q000187:drug effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D017238:Genes, Helminth / Q000187:drug effects; D049751:Genome, Helminth; D020411:Oligonucleotide Array Sequence Analysis; D018510:RNA, Helminth / Q000187:drug effects; D013343:Styrenes / Q000008:administration & dosage / Q000633:toxicity*
D004785:Environmental Pollutants; D018510:RNA, Helminth; D013343:Styrenes; C014138:octachlorostyrene