Immunomodulatory activity of a plant extract containing human papillomavirus 16-E7 protein in human monocyte-derived dendritic cells.
This study reports the immunomodulatory activity on human monocyte derived dendritic cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions.
Di Bonito
P
P
Department of Infectious Parasitic and Immune-mediated Diseases, Superior Institute of Health, Rome, Italy. paola.dibonito@iss.it
Grasso
F
F
Mangino
G
G
Massa
S
S
Illiano
E
E
Franconi
R
R
Fanales-Belasio
E
E
Falchi
M
M
Affabris
E
E
Giorgi
C
C
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D017951:Antigen Presentation; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005822:Genetic Vectors; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010936:Plant Extracts / Q000276:immunology* / Q000302:isolation & purification; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D010936:Plant Extracts; D011994:Recombinant Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D010047:Ovalbumin