title: A modified excised leaf disc method to estimate the toxicity of slow- and fast-acting reduced-risk acaricides to mites.
abstract: The "modified excised leaf disc method" is based on leaf discs that fit tightly the bottom halves of 50-mm petri dishes. The bottom half of each petri dish is covered with wet cotton wool to prolong leaf freshness. The side wall of each bottom half has a small hole to allow the petiole of the leaf disc to protrude outside the petri dish. The top half of each petri dish has a 28-mm (diameter) window. For phytophagous mites the window is covered with a 40-microm mesh Pecap polyester screen. Using this method it was possible to estimate the LC50 value of bifenazate to be 0.00413 g (AI)/liter and the LC50 value of spirodiclofen to be 0.40050 g (AI)/liter to the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). The method reduces losses due to escapees and allows observations to be made as long as 9 d after treatment.
authors.affiliation: Horticultural Research and Development Centre, Agriculture and Agri-Food Canada, St. Jean-sur-Richelieu, Quebec, Canada. noubar.bostanian@agr.gc.ca
authors.forename: Noubar J
authors.initials: NJ
authors.lastname: Bostanian
authors.forename: Serge
authors.initials: S
authors.lastname: Beudjekian
authors.forename: Erin
authors.initials: E
authors.lastname: McGregor
authors.forename: Gaétan
authors.initials: G
authors.lastname: Racette
mesh_terms: D056810:Acaricides; D000818:Animals; D007928:Lethal Dose 50; D008925:Mites; D018515:Plant Leaves; D018675:Toxicity Tests / Q000379:methods
chemical_list: D056810:Acaricides