title: Automated micronucleus (MN) scoring for population triage in case of large scale radiation events.
abstract: OBJECTIVE
  In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals (> or =1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automated micronucleus (MN) scoring.
  
  
  METHODS
  Using the MN software module developed by MetaSystems specifically for the Metafer4 platform, about 60 blood samples can be scored in one day. Standard dose response curves were determined for manual and automated MN scoring.
  
  
  RESULTS
  The automated MN assay results were closely correlated with MN yields obtained with the manual procedure. A dose of 1 Gy can be estimated with an uncertainty of 0.2 Gy. Corrections for false positives and false negatives by visual inspection of the image gallery did not result in an improved accuracy or reproducibility. To test the automated MN assay in a multicenter setting, an inter-laboratory comparison was performed whereby irradiated blood samples were processed in Ghent University (Belgium) and BfS (Bundesamt fuer Strahlenschutz; Germany). Both laboratories obtained comparable results.
  
  
  CONCLUSIONS
  These results confirm the efficacy of the automated MN assay for fast population triage in a multicenter setting, in the case of large radiation accidents.
authors.affiliation: Department of Basic Medical Sciences, Ghent University, Ghent, Belgium.
authors.forename: Petra
authors.initials: P
authors.lastname: Willems
authors.forename: Liezel
authors.initials: L
authors.lastname: August
authors.forename: Jacobus
authors.initials: J
authors.lastname: Slabbert
authors.forename: Horst
authors.initials: H
authors.lastname: Romm
authors.forename: Ursula
authors.initials: U
authors.lastname: Oestreicher
authors.forename: Hubert
authors.initials: H
authors.lastname: Thierens
authors.forename: Anne
authors.initials: A
authors.lastname: Vral
mesh_terms: D000328:Adult; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007211:Indoles; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D018788:Radioactive Hazard Release; D014218:Triage
chemical_list: D007211:Indoles; C007293:DAPI