title: Pulsed EPR investigations of the Mo(V) centers of the R55Q and R55M variants of sulfite dehydrogenase from Starkeya novella.
abstract: Continuous-wave and pulsed electron paramagnetic resonance (EPR) spectroscopy have been used to characterize two variants of bacterial sulfite dehydrogenase (SDH) from Starkeya novella in which the conserved active-site arginine residue (R55) is replaced by a neutral amino acid residue. Substitution by the hydrophobic methionine residue (SDH(R55M)) has essentially no effect on the pH dependence of the EPR properties of the Mo(V) center, even though the X-ray structure of this variant shows that the methionine residue is rotated away from the Mo center and a sulfate anion is present in the active-site pocket (Bailey et al. in J Biol Chem 284:2053-2063, 2009). For SDH(R55M) only the high-pH form is observed, and samples prepared in H(2)(17)O-enriched buffer show essentially the same (17)O hyperfine interaction and nuclear quadrupole interaction parameters as SDH(WT) enzyme. However, the pH dependence of the EPR spectra of SDH(R55Q), in which the positively charged arginine is replaced by the neutral hydrophilic glutamine, differs significantly from that of SDH(WT). For SDH(R55Q) the blocked form with bound sulfate is generated at low pH, as verified by (33)S couplings observed upon reduction with (33)S-labeled sulfite. This observation of bound sulfate for SDH(R55Q) supports our previous hypothesis that sulfite-oxidizing enzymes can exhibit multiple pathways for electron transfer and product release (Emesh et al. in Biochemistry 48:2156-2163, 2009). At pH > or = 8 the high-pH form dominates for SDH(R55Q).
authors.affiliation: Centre for Metals in Biology, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Trevor D
authors.initials: TD
authors.lastname: Rapson
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Astashkin
authors.forename: Kayunta
authors.initials: K
authors.lastname: Johnson-Winters
authors.forename: Paul V
authors.initials: PV
authors.lastname: Bernhardt
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kappler
authors.forename: Arnold M
authors.initials: AM
authors.lastname: Raitsimring
authors.forename: John H
authors.initials: JH
authors.lastname: Enemark
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology*; D019943:Amino Acid Substitution; D020134:Catalytic Domain; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008024:Ligands; D008982:Molybdenum; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D050902:Sulfite Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D008024:Ligands; D050505:Mutant Proteins; D008982:Molybdenum; D050902:Sulfite Dehydrogenase