Lymphatic channel density in colorectal adenocarcinoma.
OBJECTIVE
The purpose of this study was to count the number of lymphatic channels present in colorectal adenocarcinoma and correlate it with site, size, and stage of tumor, lymph node metastasis.


METHODS
A total of 29 cases of colorectal carcinomas were retrieved from the archives of the pathology department, School of Medical Sciences. One paraffin block containing tumor was selected from each case. Sections of three to five micron thickness were cut from this paraffin block and stained using the monoclonal antibody D2-40[DAKO] specifically to stain lymphatic channel endothelium in normal and neoplastic tissue. The highest number of lymphatic channels in an area of 0.196 mm2 [high power field] was counted in each tumor using NIKON microscope. These findings were correlated with the clinical parameters and also with lymph node metastasis. Statistical software used: SPSS version 11.


RESULTS
The highest density of lymphatic channels in colorectal carcinoma was counted after identifying the appropriate "hot spot". The lymphatic channel density was in the range of 15 - 50/ 0.196 mm 2 [high power field]. There was poor association of this lymphatic channel density with site, size, and stage of tumor and also with lymph node metastasis. This result is in concordance with results of studies done elsewhere.


CONCLUSIONS
In this study no significant association was seen between lymphatic channel density and site, size, stage and lymph node metastasis in colorectal carcinoma. This indicates that lymphatic channel proliferation does not influence tumor aggressiveness. Further studies are needed to validate our findings.
Naik
Venkatesh R
VR
Department of Pathology, School of Medical Sciences, Health Campus, Universiti sains Malaysia, Kubang kerian, 16150 Kelantan, Malaysia.
Jaafar
Hasnan
H
Seng
Ch'ng Ewe
CE
D000230:Adenocarcinoma / Q000473:pathology*; D001699:Biometry / Q000379:methods; D015179:Colorectal Neoplasms / Q000473:pathology*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D042601:Lymphatic Vessels / Q000473:pathology*; D008853:Microscopy / Q000379:methods