title: Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern.
abstract: A Gram-stain-variable, motile, moderately halophilic bacterial strain, CHL-21(T), was isolated from a marine solar saltern and its taxonomic position was investigated using a polyphasic approach. Optimal growth of strain CHL-21(T) occurred at 30-37 °C, at pH 7.0-7.5 and in the presence of 5-10 % (w/v) NaCl. In phylogenetic trees based on 16S rRNA gene sequences, strain CHL-21(T) fell within the cluster comprising members of the genera Oceanobacillus, Ornithinibacillus and Paucisalibacillus. Strain CHL-21(T) exhibited 97.1-97.2 % 16S rRNA gene sequence similarity to the type strains of the two subspecies of Oceanobacillus oncorhynchi and 92.0-94.7 % 16S rRNA gene sequence similarity to the type strains of other members of the genus Oceanobacillus and members of the genera Ornithinibacillus and Paucisalibacillus. Mean DNA-DNA reassociation values between strain CHL-21(T) and the type strains of the two subspecies of Oceanobacillus oncorhynchi were 19-21 %. The cell-wall peptidoglycan of strain CHL-21(T) was based on meso-diaminopimelic acid, MK-7 was the predominant menaquinone, and anteiso-C(15 : 0) and anteiso-C(17 : 0) were the major fatty acids. The DNA G+C content was 39.8 mol%. Differential phenotypic properties, including facultatively anaerobic growth and acid production from substrates, together with its phylogenetic and genetic distinctiveness, demonstrated that strain CHL-21(T) is distinguishable from recognized Oceanobacillus species. On the basis of data presented, strain CHL-21(T) represents a novel species within the genus Oceanobacillus, for which the name Oceanobacillus locisalsi sp. nov. is proposed; the type strain is CHL-21(T) (=KCTC 13253(T) =CCUG 56608(T)).
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
authors.affiliation: Department of Microbiology, College of Medicine, Chungang University, 221 Heukseok-dong, Seoul, Republic of Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7