title: Therapeutic and persistent efficacy of a long-acting (LA) formulation of ivermectin against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) and sera concentration through time in treated cattle.
abstract: The concentration-time profile, therapeutic, and persistent efficacy of a single subcutaneous injection of cattle with a long-acting (LA) formulation of ivermectin at a concentration of 630microg/kg of body weight were determined against Rhipicephalus (Boophilus) microplus. Ivermectin sera concentration in treated cattle increased to 13.0ppb within 1d after treatment, and peaked at 26.2ppb at 11d post-treatment. Ivermectin sera levels remained above the threshold level for control of feeding ticks (>or=8ppb) for 42.6d after treatment. Therapeutic efficacy of ticks on treated animals was >99.9%, and tick number, index of fecundity, engorgement weight, and egg mass weight of ticks from treated animals remained dramatically less than ticks from untreated animals. Tick number and reproductive capacity of ticks infested on treated animals at 14 and 28d post-treatment were less than for ticks on untreated animals, whereas engorgement weight and egg mass weight of treated ticks remained lower than that of untreated ticks 49d post-treatment. However, the level of control against ticks infested at 14d after treatment (99.9%) was the only post-treatment infestation interval that provided the required 99% control necessary for use in the U.S. tick eradication program. The 14d post-treatment infestation was also the only interval at which infested ticks were exposed to ivermectin levels above the threshold level of 8ppb for the entire parasitic development period. Cattle would have to be treated at intervals of no more than 31d apart to ensure that no viable ticks could reach repletion and detach from the host. Although this treatment interval is >2-fold longer than the present treatment requirement (14d), it is dramatically less than the label claim for the LA ivermectin formulation of 75d of prevention against re-infestation.
authors.affiliation: USDA, ARS, SPA, Cattle Fever Tick Research Lab., Moore Air Base, Bldg. 6419, Edinburg, TX 78541, USA. ronald.davey@ars.usda.gov
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Davey
authors.forename: J Mathews
authors.initials: JM
authors.lastname: Pound
authors.forename: J Allen
authors.initials: JA
authors.lastname: Miller
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Klavons
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy*; D004478:Ectoparasitic Infestations / Q000188:drug therapy / Q000662:veterinary*; D005260:Female; D007559:Ivermectin / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D048494:Rhipicephalus / Q000187:drug effects; D013997:Time Factors
chemical_list: D000977:Antiparasitic Agents; D007559:Ivermectin