Counting single molecules in sub-nanolitre droplets.
We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.
Rane
T D
TD
Johns Hopkins University, Department of Biomedical Engineering, 3400 N. Charles St., Clark Hall, Baltimore, MD, USA. tushar@jhu.edu
Puleo
C M
CM
Liu
K J
KJ
Zhang
Y
Y
Lee
A P
AP
Wang
T H
TH
D004247:DNA / Q000032:analysis* / Q000648:ultrastructure; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000032:analysis* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
D012996:Solutions; D004247:DNA