[Effects of cycloartocarpin A and artocarpin extracted from Fructus Artocarpin Heterophylli on apoptosis of SMMC-7721 and SGC-7901 cells].
OBJECTIVE
To investigate the effects of cycloartocarpin A (ACR-2) and artocarpin (ACR-3), monomeric compounds isolated from Fructus Artocarpi Heterophylli, on apoptosis of SMMC-7721 and SGC-7901 cell lines.


METHODS
SMMC-7721 and SGC-7901 cells were routinely cultured, and divided into experiment group and control group. The SMMC-7721 cells were treated with different concentrations of ACR-2 (3.46 x 10(-3), 13.82 x (-3), 55.30 x 10(-3) mmol/L) and ACR-3 (6.88 x 10(-3), 27.52 x 10(-3), 110.09 x 10(-3) mmol/L), and the SGC-7901 cells were also treated with different concentrations of ACR-2 (8.06 x 10(-3), 32.26 x 10(-3), 129.03 x 10(-3) mmol/L) and ACR-3 (2.87 x 10(-3), 11.47 x 10(-3), 45.87 x 10(-3) mmol/L), with PBS (DMSO<0.1%) as control treatment. Cell apoptosis was measured by double labeled staining with Hoechst33342/propidium iodide (PI) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry.


RESULTS
ACR-2 and ACR-3 could induce apoptosis of SMMC-7721 and SGC-7901 cells. Some of SMMC-7721 and SGC-7901 cells demonstrated typical apoptosis after being treated with ACR-2 and ACR-3. Hoechst33342/PI staining showed that cells were fraught with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared light blue. TUNEL showed that cells permeated with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared brown. Less apoptotic cells were observed in negative control group, and the nuclear appeared light blue. The apoptosis rates of SMMC-7721 and SGC-7901 cells in the ACR-2 and ACR-3 treated groups were significant higher than those in the control group (P<0.05, P<0.01).


CONCLUSIONS
ACR-2 and ACR-3 can induce apoptosis of SMMC-7721 and SGC-7901 cells.
Yang
Yan-long
YL
Department of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Hou
Ai-jun
AJ
Zhang
Hui-qing
HQ
Shen
Hong
H
Li
Qiu-shuang
QS
Zhang
Cong-cong
CC
Zhu
Guo-fu
GF
D017209:Apoptosis / Q000187:drug effects*; D031622:Artocarpus / Q000737:chemistry; D045744:Cell Line, Tumor / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D037241:Mannose-Binding Lectins / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D037121:Plant Lectins / Q000494:pharmacology*
D037241:Mannose-Binding Lectins; D010936:Plant Extracts; D037121:Plant Lectins; C090683:artocarpin lectin