title: Actobiotics as a novel method for cytokine delivery.
abstract: Interleukin-10 (IL-10) is central in immune downregulation, but so far its use in inflammatory diseases remains cumbersome. For treatment of inflammatory bowel disease, adequate amounts of IL-10 must reach the intestinal lining. Systemic injection of a pharmacologically active doses of recombinant human (rh) IL-10 results in very low mucosal levels of protein and severe toxicity and side effects. In animal models, topical and active delivery of IL-10 by ingestion of recombinant Lactococcus lactis (L. lactis) was shown to be a valuable alternative. Starting thereof we have developed a novel pharmaceutical platform. Our expertise and TopAct (topical and active) delivery technology allows use of recombinant L. lactis- ActoBiotics- in clinical practice. Here we discuss the development of recombinant L. lactis for intestinal delivery of rhIL-10 in humans.
authors.affiliation: ActoGeniX NV, Zwijnaarde, Belgium. lothar.steidler@actogenix.com
authors.forename: Lothar
authors.initials: L
authors.lastname: Steidler
authors.forename: Pieter
authors.initials: P
authors.lastname: Rottiers
authors.forename: Bernard
authors.initials: B
authors.lastname: Coulie
mesh_terms: D000818:Animals; D017321:Clinical Trials, Phase I as Topic; D017322:Clinical Trials, Phase II as Topic; D016503:Drug Delivery Systems; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism; D016753:Interleukin-10 / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D016753:Interleukin-10