Preferential transfer of certain plasma membrane proteins onto T and B cells by trogocytosis.
T and B cells capture antigens via membrane fragments of antigen presenting cells (APC) in a process termed trogocytosis. Whether (and how) a preferential transfer of some APC components occurs during trogocytosis is still largely unknown. We analyzed the transfer onto murine T and B cells of a large panel of fluorescent proteins with different intra-cellular localizations in the APC or various types of anchors in the plasma membrane (PM). Only the latter were transferred by trogocytosis, albeit with different efficiencies. Unexpectedly, proteins anchored to the PM's cytoplasmic face, or recruited to it via interaction with phosphinositides, were more efficiently transferred than those facing the outside of the cell. For proteins spanning the PM's whole width, transfer efficiency was found to vary quite substantially, with tetraspanins, CD4 and FcRgamma found among the most efficiently transferred proteins. We exploited our findings to set immunodiagnostic assays based on the capture of preferentially transferred components onto T or B cells. The preferential transfer documented here should prove useful in deciphering the cellular structures involved in trogocytosis.
Daubeuf
Sandrine
S
CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
Aucher
Anne
A
Bordier
Christine
C
Salles
Audrey
A
Serre
Laurent
L
Gaibelet
Gérald
G
Faye
Jean-Charles
JC
Favre
Gilles
G
Joly
Etienne
E
Hudrisier
Denis
D
D017951:Antigen Presentation; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D021381:Protein Transport / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D008565:Membrane Proteins