The effect of interferon-gamma and lipopolysaccharide on the growth of Francisella tularensis LVS in murine macrophage-like cell line J774.
BACKGROUND
Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells.


OBJECTIVE
To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide.


RESULTS
Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection.


CONCLUSIONS
Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis.
Holická
Monika
M
Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Králové, Czech Republic.
Novosad
Jakub
J
Loudová
Martina
M
Kudlová
Manuela
M
Krejsek
Jan
J
D000818:Animals; D002460:Cell Line; D005604:Francisella tularensis / Q000254:growth & development*; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins
D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007371:Interferon-gamma