title: [Cultivation of pathogenic free-living amoebae].
abstract: The isolation and culture of pathogenic free-living amoebae are useful in the diagnosis and research. This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
authors.affiliation: Department of Etiologic Biology, The Second Military Medical University, Shanghai, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Peng
authors.forename: Huai-Min
authors.initials: HM
authors.lastname: Zhu
mesh_terms: D000048:Acanthamoeba / Q000254:growth & development* / Q000302:isolation & purification* / Q000472:pathogenicity; D000656:Amoeba / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D006801:Humans