title: Acute mucosal pathogenesis of feline immunodeficiency virus is independent of viral dose in vaginally infected cats.
abstract: BACKGROUND
  The mucosal pathogenesis of HIV has been shown to be an important feature of infection and disease progression. HIV-1 infection causes depletion of intestinal lamina propria CD4+ T cells (LPL), therefore, intestinal CD4+ T cell preservation may be a useful correlate of protection in evaluating vaccine candidates. Vaccine studies employing the cat/FIV and macaque/SIV models frequently use high doses of parenterally administered challenge virus to ensure high plasma viremia in control animals. However, it is unclear if loss of mucosal T cells would occur regardless of initial viral inoculum dose. The objective of this study was to determine the acute effect of viral dose on mucosal leukocytes and associated innate and adaptive immune responses.
  
  
  RESULTS
  Cats were vaginally inoculated with a high, middle or low dose of cell-associated and cell-free FIV. PBMC, serum and plasma were assessed every two weeks with tissues assessed eight weeks following infection. We found that irrespective of mucosally administered viral dose, FIV infection was induced in all cats. However, viremia was present in only half of the cats, and viral dose was unrelated to the development of viremia. Importantly, regardless of viral dose, all cats experienced significant losses of intestinal CD4+ LPL and CD8+ intraepithelial lymphocytes (IEL). Innate immune responses by CD56+CD3- NK cells correlated with aviremia and apparent occult infection but did not protect mucosal T cells. CD4+ and CD8+ T cells in viremic cats were more likely to produce cytokines in response to Gag stimulation, whereas aviremic cats T cells tended to produce cytokines in response to Env stimulation. However, while cell-mediated immune responses in aviremic cats may have helped reduce viral replication, they could not be correlated to the levels of viremia. Robust production of anti-FIV antibodies was positively correlated with the magnitude of viremia.
  
  
  CONCLUSIONS
  Our results indicate that mucosal immune pathogenesis could be used as a rapid indicator of vaccine success or failure when combined with a physiologically relevant low dose mucosal challenge. We also show that innate immune responses may play an important role in controlling viral replication following acute mucosal infection, which has not been previously identified.
authors.affiliation: Center for Comparative Medicine and Translational Research, Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA. KEHoward25@gmail.com
authors.forename: Kristina E
authors.initials: KE
authors.lastname: Howard
authors.forename: Stacie K
authors.initials: SK
authors.lastname: Reckling
authors.forename: Erin A
authors.initials: EA
authors.lastname: Egan
authors.forename: Gregg A
authors.initials: GA
authors.lastname: Dean
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002371:Cat Diseases / Q000276:immunology* / Q000821:virology; D002415:Cats; D016207:Cytokines / Q000378:metabolism; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000472:pathogenicity*; D007413:Intestinal Mucosa / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D016180:Lentivirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014621:Vagina / Q000821:virology*; D019562:Viral Load; D014766:Viremia
chemical_list: D016207:Cytokines