title: [Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody].
abstract: OBJECTIVE
  To evaluate and comparison of 2 commercial ELISA kits (Vrion/Serion kit and IBL kit) which ELISA kit will be used in sero-epidemiological survey in China in 2006 by Plaque Reduction Neutralization Test (PRNT).
  
  
  METHODS
  52 serum panel which contain different levels of measles antibody were used.
  
  
  RESULTS
  The results showed that the sensitivity, PPV and accurate of Virion/Serion kit achieved highest score when taking PRNT as "gold standard". Virion/Serion kit showed good relationship with PRNT titer: the correlation coefficients are 0.878 (P<0.01). IBL kit get lower correlation coefficients 0.850 (P<0.01). The test value and mean of unit of serum antibody increased along with neutralizing titers increased.
  
  
  CONCLUSIONS
  The Virion/Serion kit is good in sensitivity and specificity. It has quantitative for single well. The kit is suitable for sera epidemiology survey to detection of automatic workstation in China.
authors.affiliation: World Health Organization Western Pacific Region Regional Reference Measles Laboratory State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Nai-ying
authors.initials: NY
authors.lastname: Mao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Jiang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008457:Measles / Q000097:blood / Q000276:immunology* / Q000821:virology; D008459:Measles virus / Q000276:immunology; D009500:Neutralization Tests; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic