title: Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.
  
  
  METHODS
  ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment.
  
  
  RESULTS
  Inhibitory effect of EGCG (200 micromol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At > or = 50 micromol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05).
  
  
  CONCLUSIONS
  EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.
authors.affiliation: Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN 37208, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Mohamed
authors.initials: M
authors.lastname: Al-Hendy
authors.forename: Gloria
authors.initials: G
authors.lastname: Richard-Davis
authors.forename: Valerie
authors.initials: V
authors.lastname: Montgomery-Rice
authors.forename: Chakradhari
authors.initials: C
authors.lastname: Sharan
authors.forename: Veera
authors.initials: V
authors.lastname: Rajaratnam
authors.forename: Anjali
authors.initials: A
authors.lastname: Khurana
authors.forename: Ayman
authors.initials: A
authors.lastname: Al-Hendy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D005260:Female; D007889:Leiomyoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D000975:Antioxidants; D018809:Proliferating Cell Nuclear Antigen; D002392:Catechin; C045651:epigallocatechin gallate; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4