title: Collagen extracts derived from young and aged mice demonstrate different structural properties and cellular effects in three-dimensional gels.
abstract: Three-dimensional (3D) type I collagen gels are increasingly utilized to simulate extracellular matrix (ECM) in vivo, but little is known about the effects of age on this model. Collagen was extracted from young (4-6 months) and aged (20-24 months) mice tails and compared. The collagens appeared similar by electrophoresis. However, relative to young, aged collagen formed fibrils slower and generated 3D gels with smaller diameter, less dense fibrils (75 vs 34 nm diameter and 8 vs 3.5% area, for young and aged respectively, p < 0.02). Correspondingly, aged collagen gels were more malleable and contractible (5% vs 19% compression, p < .02, and 73% vs 15.5% area, p < .01, for young and aged, respectively). Fibroblasts cultured within young and aged collagen gels had differential expression of a limited number of genes and proteins corresponding to specific integrins and matrix components. In summary, collagen extracted from young and aged mice is an effective means to examine the influence of aging on functional properties of ECM that are relevant in vivo.
authors.affiliation: Department of Medicine, Harborview Medical Center, University of Washington, Box 359625, 325 Ninth Avenue, Seattle, WA 98104, USA.
authors.forename: Mamatha
authors.initials: M
authors.lastname: Damodarasamy
authors.forename: Robert B
authors.initials: RB
authors.lastname: Vernon
authors.forename: Nathan
authors.initials: N
authors.lastname: Karres
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Chang
authors.forename: Daniella
authors.initials: D
authors.lastname: Bianchi-Frias
authors.forename: Peter S
authors.initials: PS
authors.lastname: Nelson
authors.forename: May J
authors.initials: MJ
authors.lastname: Reed
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D024042:Collagen Type I / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D015394:Molecular Structure
chemical_list: D024042:Collagen Type I