Efficient production of 2-oxobutyrate from 2-hydroxybutyrate by using whole cells of Pseudomonas stutzeri strain SDM.
2-Oxobutyrate is an important intermediate in the chemical, drug, and food industries. Whole cells of Pseudomonas stutzeri SDM, containing NAD-independent lactate dehydrogenases, effectively converted 2-hydroxybutyrate into 2-oxobutyrate. Under optimal conditions, the biocatalytic process produced 2-oxobutyrate at a high concentration (44.4 g liter(-1)) and a high yield (91.5%).
Gao
Chao
C
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Zhang
Wen
W
Lv
Chuanjuan
C
Li
Lixiang
L
Ma
Cuiqing
C
Hu
Chunhui
C
Xu
Ping
P
D001709:Biotechnology / Q000379:methods; D002087:Butyrates / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D044223:Pseudomonas stutzeri / Q000378:metabolism*
D002087:Butyrates; D006885:Hydroxybutyrates; C005087:alpha-ketobutyric acid; D007770:L-Lactate Dehydrogenase