Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS).
Oxycodone is a semisynthetic opioid analgesic used in pain management. In the following method, gas chromatography-mass spectrometry (GC-MS) is used to determine the presence and concentration of the drug in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted using mixed-mode solid phase extraction and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) producing trimethylsilyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.
Goldberger
Bruce A
BA
Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Chronister
Chris W
CW
Merves
Michele L
ML
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010098:Oxycodone / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D014297:Trimethylsilyl Compounds / Q000737:chemistry
D014297:Trimethylsilyl Compounds; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; D010098:Oxycodone