Polymorphism (ALA16VAL) correlates with regional lymph node status in breast cancer.
We studied the possible association between Ala16Val manganese-dependent superoxide dismutase (MnSOD) gene genotypes and breast cancer lymph node status because previous investigations suggested an association between the AA genotype and breast cancer. We included 281 women (188 controls and 93 cases of invasive breast cancer with axillary lymph node metastasis (LN+) and without lymph node metastasis (LN-). DNA was extracted from paraffin-embedded tumor tissue or peripheral blood leukocytes, and MnSOD polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism techniques. In addition, the immunohistochemical profile (p53, Ki-67 and estrogen/progesterone receptors) was also compared between invasive breast cancer groups and different MnSOD genotypes. The frequency of the VV genotype was higher in the LN+ group than in the control and LN- groups (chi(2)=5.081, P=0.02). Subjects with LN+ breast cancer (LN+ group) showed a higher incidence of VV genotype carriers associated with positive Ki-67 marker. Subjects with LN+ breast cancer (LN+ group) showed a higher incidence of VV genotype carriers associated with negative p53 marker. Despite the fact that the AA genotype is well established as being associated with an increased risk of breast cancer, the VV genotype may be associated with a higher metastatic potential, suggesting that MnSOD imbalance is the condition associated with carcinogenesis.
Bica
Claudia Giuliano
CG
Programa de Pós-Graduação em Patologia, Universidade Federal de Ciências Médicas de Porto Alegre, Rua Sarmento Leite, 245, Porto Alegre 90050-170, Brazil.
da Silva
Leonardo Leiria de Moura
LL
Toscani
Nadima Vieira
NV
Zettler
Cláudio Galleano
CG
Gottlieb
Maria Gabriela do Valle
MG
Alexandre
Cláudio Osmar Pereira
CO
Graudenz
Márcia Silveira
MS
Mânica da Cruz
Ivana Beatrice
IB
D000409:Alanine / Q000235:genetics*; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008198:Lymph Nodes / Q000201:enzymology / Q000473:pathology*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics; D014633:Valine / Q000235:genetics*
D013482:Superoxide Dismutase; D014633:Valine; D000409:Alanine