Metabolomic study of myocardial ischemia and intervention effects of Compound Danshen Tablets in rats using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
Myocardial ischemia (MI) is a worldwide epidemic. Compound Danshen Tablets (CDTs), an herbal compound preparation, are widely used to treat MI in China. In this study, we aimed to explore novel biomarkers to increase the understanding of MI and investigate therapeutic mechanisms of CDT by using a metabolomic approach. Plasma extracts from sham, MI model, CDT- and western medicines (isosorbide dinitrate, verapamil, propranolol, captopril, and trimethazine)-treated rats were analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The orthogonal partial least square (OPLS) model was built to find metabolites expressed in significantly different amounts between MI and sham rats. Meanwhile, partial least squares discriminant analysis (PLS-DA) was used to investigate CDT's protective effects. The results showed that CDT presented protective effects on MI by reversing potential biomarkers to sham levels, especially for the four metabolites in the pathway of purine metabolism (hypoxanthine, xanthine, inosine and allantoin).
Lv
Yonghai
Y
Department of Medicinal Chemistry of Nature Product, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
Liu
Xinru
X
Yan
Shikai
S
Liang
Xu
X
Yang
Yun
Y
Dai
Weixing
W
Zhang
Weidong
W
D000481:Allantoin / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002317:Cardiovascular Agents / Q000378:metabolism / Q000494:pharmacology*; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000494:pharmacology*; D019271:Hypoxanthine / Q000097:blood; D007288:Inosine / Q000097:blood; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics / Q000379:methods*; D017202:Myocardial Ischemia / Q000097:blood / Q000188:drug therapy*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza; D021241:Spectrometry, Mass, Electrospray Ionization; D013607:Tablets; D019820:Xanthine / Q000097:blood
D015415:Biomarkers; D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D013607:Tablets; D019820:Xanthine; D019271:Hypoxanthine; D000481:Allantoin; D007288:Inosine