title: Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System.
abstract: This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.
authors.affiliation: Department of Bionano Technology, Kyungwon University, Sungnam-si, Kyungki-do 461-701, South Korea. seong_an@gmail.com
authors.forename: S S A
authors.initials: SS
authors.lastname: An
authors.forename: K T
authors.initials: KT
authors.lastname: Lim
authors.forename: H J
authors.initials: HJ
authors.lastname: Oh
authors.forename: B S
authors.initials: BS
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Zukic
authors.forename: Y R
authors.initials: YR
authors.lastname: Ju
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: E
authors.initials: E
authors.lastname: Welker
mesh_terms: D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018620:PrPSc Proteins / Q000097:blood* / Q000737:chemistry; D055503:Protein Multimerization; D012608:Scrapie / Q000097:blood*
chemical_list: D018620:PrPSc Proteins