title: Chronic kidney disease induced in mice by reversible unilateral ureteral obstruction is dependent on genetic background.
abstract: Chronic kidney disease (CKD) begins with renal injury; the progression thereafter depends upon a number of factors, including genetic background. Unilateral ureteral obstruction (UUO) is a well-described model of renal fibrosis and as such is considered a model of CKD. We used an improved reversible unilateral ureteral obstruction (rUUO) model in mice to study the strain dependence of development of CKD after obstruction-mediated injury. C57BL/6 mice developed CKD after reversal of three or more days of ureteral obstruction as assessed by blood urea nitrogen (BUN) measurements (>40 mg/dl). In contrast, BALB/c mice were resistant to CKD with up to 10 days ureteral obstruction. During rUUO, C57BL/6 mice exhibited pronounced inflammatory and intrinsic proliferative cellular responses, disruption of renal architecture, and ultimately fibrosis. By comparison, BALB/c mice had more controlled and measured extrinsic and intrinsic responses to injury with a return to normal within several weeks after release of ureteral obstruction. Our findings provide a model that allows investigation of the genetic basis of events during recovery from injury that contribute to the development of CKD.
authors.affiliation: Section of Nephrology, Department of Medicine, University of Chicago, 5841 South Maryland Ave., Chicago, IL 60637, USA. tpuri@medicine.bsd.uchicago.edu
authors.forename: Tipu S
authors.initials: TS
authors.lastname: Puri
authors.forename: Mohammed I
authors.initials: MI
authors.lastname: Shakaib
authors.forename: Anthony
authors.initials: A
authors.lastname: Chang
authors.forename: Liby
authors.initials: L
authors.lastname: Mathew
authors.forename: Oladunni
authors.initials: O
authors.lastname: Olayinka
authors.forename: Andrew W M
authors.initials: AW
authors.lastname: Minto
authors.forename: Menaka
authors.initials: M
authors.lastname: Sarav
authors.forename: Bradley K
authors.initials: BK
authors.lastname: Hack
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Quigg
mesh_terms: D000818:Animals; D020022:Genetic Predisposition to Disease; D007676:Kidney Failure, Chronic / Q000209:etiology* / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014517:Ureteral Obstruction / Q000150:complications*