title: Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA.
abstract: Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis (TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine industry. Previously, we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA polymerase (RdRP) gene of TGEV with more than 95% of virus inhibition in vitro. In this study, inhibition of the TGEV replication by pEGFP-U6/P1 and pEGFP-U6/P2 was tested in mini-pigs. SPF mini-pigs at 25 days old were injected with the shRNA-expressing plasmids and then infected with TGEV. The results from the analyses of clinical signs, histopathology, indirect immunofluorescence (IIF) and RT-PCR show that the two shRNA-expressing plasmids could significantly decrease the quantity of TGEV in different organs and protect mini-pigs from TGEV infection. These findings illustrate the prospect for TGEV-specific shRNAs to be new anti-TGEV agents.
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Science, 300 Jungong Road, Shanghai 200090, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Zhou
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Yijia
authors.initials: Y
authors.lastname: Yan
authors.forename: Piren
authors.initials: P
authors.lastname: Chen
authors.forename: Dezhong
authors.initials: D
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Mou
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Daoliang
authors.initials: D
authors.lastname: Lan
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000825:Animal Structures / Q000473:pathology / Q000821:virology; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D001688:Biological Products / Q000235:genetics / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005761:Gastroenteritis, Transmissible, of Swine / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use*; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors / Q000235:genetics; D013552:Swine; D013556:Swine, Miniature; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000502:physiology*; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D001688:Biological Products; D034741:RNA, Small Interfering; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase