title: Sequence polymorphism and PCR-restriction fragment length polymorphism analysis of the flagellin gene of Burkholderia pseudomallei.
abstract: Four flagellin allelic types (I to IV) of Burkholderia pseudomallei were identified based on their sequence variation and restriction fragment length polymorphism (RFLP) analysis of the amplified flagellin gene. Flagellin allelic type I was the most predominantly (75.0%) found among the 100 clinical isolates of B. pseudomallei investigated in this study.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. tayst@um.edu.my
authors.forename: Sun Tee
authors.initials: ST
authors.lastname: Tay
authors.forename: Pui Ching
authors.initials: PC
authors.lastname: Cheah
authors.forename: Savithiri D
authors.initials: SD
authors.lastname: Puthucheary
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D005408:Flagellin / Q000235:genetics*; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin