Human RNA polymerase I-driven reverse genetics for influenza a virus in canine cells.
We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
Suphaphiphat
Pirada
P
Novartis Vaccines and Diagnostics, 45 Sidney Street, Cambridge, MA 02139, USA.
Keiner
Bjoern
B
Trusheim
Heidi
H
Crotta
Stefania
S
Tuccino
Annunziata Barbara
AB
Zhang
Pu
P
Dormitzer
Philip R
PR
Mason
Peter W
PW
Franti
Michael
M
D000818:Animals; D002460:Cell Line; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics / Q000502:physiology*
D007252:Influenza Vaccines; D012318:RNA Polymerase I