Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures.
To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.
Lin
Hsiu-Li
HL
Department of Neurology, General Cathay Hospital, Sijhih, Taiwan.
Lin
Chien-Chung
CC
Lin
Yi-Jen
YJ
Lin
Hsiu-Chen
HC
Shih
Chwen-Ming
CM
Chen
Chi-Rong
CR
Huang
Rong-Nan
RN
Kuo
Tai-Chih
TC
D017033:Anabaena / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development*; D009391:Nephelometry and Turbidimetry / Q000379:methods* / Q000592:standards*; D013997:Time Factors