title: Triterpene saponins from Cyclamen trocopteranthum.
abstract: Two new triterpene saponins ( 1- 2) together with three known saponins, deglucocyclamin I ( 3), cyclamin ( 4), and mirabilin ( 5), were isolated from the tubers of Cyclamen trocopteranthum. They were elucidated as 3 beta- O-{4- O-[3-hydroxyl-3-methylglutaryl]- beta-D-xylopyranosyl-(1 --> 2)- beta-D-glucopyranosyl-(1 --> 4)-[ beta-D-glucopyranosyl-(1 --> 2)]- alpha-L-arabinopyranosyl}-16 alpha-hydroxy-13 beta,28-epoxy-oleanan-30-al ( 1) and 3 beta- O-{4- O-[3-hydroxyl-3-methylglutaryl]- beta-D-xylopyranosyl-(1 --> 2)-[ beta-D-glucopyranosyl-(1 --> 6)]- beta-D-glucopyranosyl-(1 --> 4)-[ beta-D-glucopyranosyl-(1 --> 2)]- alpha-L-arabinopyranosyl}-16 alpha-hydroxy-20,30-lactone-olean-12-ene ( 2). Their structures were characterized mainly by a combination of 1D- and 2D-NMR techniques ( (1)H- (1)H COSY, TOCSY, NOESY, HSQC, and HMBC) and mass spectroscopy. Saponins 1, 3, and 4 showed a weak cytotoxic activity when tested against HT-29 and HCT 116 tumor colon cancer cells.
authors.affiliation: Laboratoire de Pharmacognosie, Unité de Molécules d'Intérêt Biologique (UMIB UPRES EA 3660), Faculté de Pharmacie, Université de Bourgogne, Dijon, France.
authors.forename: Ghezala
authors.initials: G
authors.lastname: Mihci-Gaidi
authors.forename: Suheyla
authors.initials: S
authors.lastname: Ozbey
authors.forename: Ilkay
authors.initials: I
authors.lastname: Orhan
authors.forename: Bilge
authors.initials: B
authors.lastname: Sener
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mirjolet
authors.forename: Olivier
authors.initials: O
authors.lastname: Duchamp
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mitaine-Offer
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D031824:Cyclamen / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012503:Saponins; D014315:Triterpenes