title: [Study of effect of thermolabile enterotoxin of Enterobacter cloacae on expression of cytokine genes in mice].
abstract: OBJECTIVE
  To study the role of production of several cytokines by macrophages and neutrophils in experiment.
  
  
  METHODS
  Production of cytokines--IL-2, 4, 6, 10, 12, 15 as well as interferon gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), transforming growth factor beta (TGF-beta) by macrophages and neutrophils during experiment on mice inoculated with enterotoxigenic strain of Enterobacter cloacae was measured. Expression of cytokines was determined by reverse transcription PCR and dot-hybridization on the basis of specially synthesized nucleotide sequences.
  
  
  RESULTS
  In macrophages of experimental animals inoculated with enterotoxigenic strain of E. cloacae increased production of TGF-beta, IFN-gamma and decreased level of TNF-alpha were revealed. Transcription of mRNAs of IL-15 and IL-6 was detected in macrophages and neutrophils of experimental animals at 24 h after inoculation. Transcription of mRNAs of IL-2, IL-4 and IL-10 was detected neither in macrophages nor in neutrophils.
  
  
  CONCLUSIONS
  Thermolabile enterotoxin of E. cloacae renders negative effect on IFN-gamma, promotes enhanced expression of TGF-beta and suppresses production of IL-2, IL-4, IL- 10. Absence of expression of key cytokines suggests that effect of thermolabile enterotoxin of Enterobacter bacteria excludes it from cytokine regulation of immune response during infectious process.
authors.forename: A A
authors.initials: AA
authors.lastname: Akhtarieva
authors.forename: I I
authors.initials: II
authors.lastname: Dolgushin
authors.forename: Z G
authors.initials: ZG
authors.lastname: Gabidullin
authors.forename: Iu Z
authors.initials: IuZ
authors.lastname: Gabidullin
authors.forename: A A
authors.initials: AA
authors.lastname: Kamalova
authors.forename: R M
authors.initials: RM
authors.lastname: Akhmadeev
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics*; D016972:Enterobacter cloacae / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D005786:Gene Expression Regulation; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D016207:Cytokines; D004768:Enterotoxins; D012333:RNA, Messenger