Pectin lyase from Aspergillus giganteus: comparative study of productivity of submerged fermentation on citrus pectin and orange waste.
The aim of this study was to investigate some of the factors affecting pectin lyase (PL) production by an Aspergillus giganteus strain, and to characterize this pectinolytic activity excreted into the medium. The highest activities were obtained with orange waste, citrus pectin and galacturonic acid as carbon sources. The highest activity, using citrus pectin as carbon source, was obtained in 11-day-old standing cultures, but the highest specific activity was obtained in 6.5-day-old shaken cultures, at pH 6.5 and 35 degrees C. Using orange waste as carbon source, the highest activity was observed in 8-day-old standing cultures, at pH 7.0 and 30 degrees C. Optimal assay conditions were pH 8.5-9.0 and 50 degrees C. The PL activity showed thermal stability, with half-lives of 30 and 27 min when incubated at 45 and 50 degrees C, respectively. High stability was observed at room temperature from pH 6.0 to 10.0; more than 85% of enzyme activity was preserved in this pH range. Under optimum conditions, the highest pectin lyase activity in the medium was 470 U/ml, with orange waste as carbon source.
Pedrolli
D B
DB
Biochemystry and Microbiology Department Biosciences Institute, São Paulo State University, Unesp, Caixa Postal 199, 13506-900, Brazil.
Carmona
E C
EC
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D032084:Citrus sinensis; D005656:Fungal Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010368:Pectins / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D013997:Time Factors; D014873:Water Pollutants / Q000378:metabolism*; D018508:Water Purification
D005656:Fungal Proteins; D014873:Water Pollutants; D010368:Pectins; D011133:Polysaccharide-Lyases; C027349:pectin lyase