title: Isolation, characterization, differentiation, and application of adipose-derived stem cells.
abstract: While bone marrow-derived mesenchymal stem cells are known and have been investigated for a long time, mesenchymal stem cells derived from the adipose tissue were identified as such by Zuk et al. in 2001. However, as subcutaneous fat tissue is a rich source which is much more easily accessible than bone marrow and thus can be reached by less invasive procedures, adipose-derived stem cells have moved into the research spotlight over the last 8 years.Isolation of stromal cell fractions involves centrifugation, digestion, and filtration, resulting in an adherent cell population containing mesenchymal stem cells; these can be subdivided by cell sorting and cultured under common conditions.They seem to have comparable properties to bone marrow-derived mesenchymal stem cells in their differentiation abilities as well as a favorable angiogenic and anti-inflammatory cytokine secretion profile and therefore have become widely used in tissue engineering and clinical regenerative medicine.
authors.affiliation: Department of Plastic, Hand and Reconstructive Surgery, Podbielskistrasse 380, 30659, Hannover, Germany, joernkuhbier@gmx.de.
authors.forename: Jörn W
authors.initials: JW
authors.lastname: Kuhbier
authors.forename: Birgit
authors.initials: B
authors.lastname: Weyand
authors.forename: Christine
authors.initials: C
authors.lastname: Radtke
authors.forename: Peter M
authors.initials: PM
authors.lastname: Vogt
authors.forename: Cornelia
authors.initials: C
authors.lastname: Kasper
authors.forename: Kerstin
authors.initials: K
authors.lastname: Reimers
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*