title: Myeloperoxidase content is a marker of systemic inflammation in a chronic condition: the example given by the periodontal disease in rats.
abstract: The study aimed to evaluate the suitability of myeloperoxidase (MPO) content as a local indicator of chronic inflammation, using the periodontal disease model. Anesthetized adult male Holtzman rats had their second left maxilar molar tied by a thread for 11 days and were then killed. Blood samples and photographic images from histopathological inflamed and noninflamed (contralateral) neighboring gingivomucosal specimens were collected for cell counts and MPO level analysis. Diseased animals were also treated with pharmacological tools such as the anti-inflammatory drug celecoxib or the opioid morphine. Increased blood neutrophils and local cell numbers characterized diseased animals. However, local MPO content was increased in inflamed and noninflamed tissues from diseased animals. Celecoxib and morphine reduced blood neutrophils and bilateral MPO content, but only celecoxib reduced local cell numbers in diseased animals. It is concluded that MPO content is a good indicator of a systemic rather than a local inflammation in a chronic inflammatory condition.
authors.affiliation: Department of Pharmacology, Federal University of Minas Gerais, 31270-100 Belo Horizonte, Brazil.
authors.forename: Celso Martins
authors.initials: CM
authors.lastname: Queiroz-Junior
authors.forename: Cinthia Mara da Fonseca
authors.initials: CM
authors.lastname: Pacheco
authors.forename: Allyson Henrique
authors.initials: AH
authors.lastname: Fonseca
authors.forename: André
authors.initials: A
authors.lastname: Klein
authors.forename: Marcelo Vidigal
authors.initials: MV
authors.lastname: Caliari
authors.forename: Janetti Nogueira
authors.initials: JN
authors.lastname: de Francischi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism*; D000068579:Celecoxib; D007249:Inflammation / Q000378:metabolism*; D007958:Leukocyte Count; D008297:Male; D009020:Morphine / Q000627:therapeutic use; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D010510:Periodontal Diseases / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism*; D011720:Pyrazoles / Q000627:therapeutic use; D051381:Rats; D013449:Sulfonamides / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D011720:Pyrazoles; D013449:Sulfonamides; D009020:Morphine; D009195:Peroxidase; D000068579:Celecoxib