title: Molecular detection and typing of Mycobacterium avium subspecies paratuberculosis from milk samples of dairy animals.
abstract: Genotyping of Mycobacterium avium subspecies paratuberculosis (MAP) is important for precise classification of bacterium and for understanding the molecular epidemiology. The present study reports detection and typing of the MAP from milk. On the basis of clinical signs of diarrhea and/or weakness, the dairy animals suspected for Johne's disease were screened by Ziehl-Neelsen staining of fecal samples. The milk samples from 13 selected animals were processed for DNA extraction and direct IS900 polymerase chain reaction (PCR). MAP identified by IS900 PCR was genotyped using IS1311 PCR-restriction endonuclease analysis (REA). IS900 milk PCR revealed 30.8% animals positive for MAP, including 40% of the moderate and 50% of the heavy fecal shedders. All infected animals showed Bison type MAP in IS1311 PCR-REA. IS900 PCR can be used for screening of milk for MAP; however, the method needs to be evaluated for subclinical cases. IS1311 PCR-REA results indicated the predominance of Bison type MAP in the dairy animals of this region.
authors.affiliation: Department of Epidemiology and Preventive Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India. kaur252@yahoo.com
authors.forename: Prabhdeep
authors.initials: P
authors.lastname: Kaur
authors.forename: G
authors.initials: G
authors.lastname: Filia
authors.forename: S V
authors.initials: SV
authors.lastname: Singh
authors.forename: P K
authors.initials: PK
authors.lastname: Patil
authors.forename: K S
authors.initials: KS
authors.lastname: Sandhu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003612:Dairying; D005243:Feces / Q000382:microbiology; D005260:Female; D008892:Milk / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*