Time-dependent changes in concentration of two clinically used acetylcholinesterase reactivators (HI-6 and obidoxime) in rat plasma determined by HPLC techniques after in vivo administration.
A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 +/- 1.71 microg/mL. The distribution of obidoxime was fast; the single maximum 23.62 +/- 3.563 microg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.
Zdarova Karasova
Jana
J
Department of Toxicology, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. karasova@pmfhk.cz
Novotny
Ladislav
L
Antos
Karel
K
Zivna
Helena
H
Kuca
Kamil
K
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D002801:Cholinesterase Reactivators / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007273:Injections, Intramuscular; D008297:Male; D009768:Obidoxime Chloride / Q000097:blood* / Q000493:pharmacokinetics*; D010091:Oximes / Q000097:blood* / Q000493:pharmacokinetics*; D011726:Pyridinium Compounds / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014238:Trichloroacetic Acid / Q000737:chemistry
D001798:Blood Proteins; D002801:Cholinesterase Reactivators; D007202:Indicators and Reagents; D010091:Oximes; D011726:Pyridinium Compounds; D009768:Obidoxime Chloride; D014238:Trichloroacetic Acid; C022870:asoxime chloride