title: Gain of chromosome 2p in chronic lymphocytic leukemia: significant heterogeneity and a new recurrent dicentric rearrangement.
abstract: Array-based comparative genomic hybridization (arrayCGH) studies in chronic lymphocytic leukemia (CLL) have revealed novel recurrent chromosomal imbalances, such as a gain of chromosome 2p. However, a detailed cytogenetic analysis of the 2p gain region has not been elucidated. Here, we present cytogenetic and molecular cytogenetic analysis of 16 such cases selected from a group of 200 patients with CLL based on CGH and/or arrayCGH data. We revealed significant heterogeneity of the region of gain on 2p in CLL, including a new recurrent aberration: the dicentric chromosome, dic(2;18). In our cases, the region of gain involved three genes (MYCN, REL, and ALK) and was associated with an unmutated IgVH status in 14 out of 16 cases. We consider this aberration clinically important in CLL and suggest that an examination of the gene(s) located in region of gain should be included in the routine fluorescence in situ hybridization screening method used for patients with CLL.
authors.affiliation: Department of Hemato-Oncology, University Hospital Olomouc, Czech Republic. marie.jarosova@fnol.cz
authors.forename: Marie
authors.initials: M
authors.lastname: Jarosova
authors.forename: Helena
authors.initials: H
authors.lastname: Urbankova
authors.forename: Radek
authors.initials: R
authors.lastname: Plachy
authors.forename: Tomas
authors.initials: T
authors.lastname: Papajik
authors.forename: Milena
authors.initials: M
authors.lastname: Holzerova
authors.forename: Jana
authors.initials: J
authors.lastname: Balcarkova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Pikalova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Divoky
authors.forename: Karel
authors.initials: K
authors.lastname: Indrak
mesh_terms: D000328:Adult; D000368:Aged; D002503:Centromere / Q000235:genetics*; D002869:Chromosome Aberrations; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D005260:Female; D018740:Genetic Heterogeneity; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged