title: Human RNA polymerase I-driven reverse genetics for influenza a virus in canine cells.
abstract: We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
authors.affiliation: Novartis Vaccines and Diagnostics, 45 Sidney Street, Cambridge, MA 02139, USA.
authors.forename: Pirada
authors.initials: P
authors.lastname: Suphaphiphat
authors.forename: Bjoern
authors.initials: B
authors.lastname: Keiner
authors.forename: Heidi
authors.initials: H
authors.lastname: Trusheim
authors.forename: Stefania
authors.initials: S
authors.lastname: Crotta
authors.forename: Annunziata Barbara
authors.initials: AB
authors.lastname: Tuccino
authors.forename: Pu
authors.initials: P
authors.lastname: Zhang
authors.forename: Philip R
authors.initials: PR
authors.lastname: Dormitzer
authors.forename: Peter W
authors.initials: PW
authors.lastname: Mason
authors.forename: Michael
authors.initials: M
authors.lastname: Franti
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics / Q000502:physiology*
chemical_list: D007252:Influenza Vaccines; D012318:RNA Polymerase I