title: A protocol for the production of KLRG1 tetramer.
abstract: Killer cell lectin-like receptor G1 (KLRG1) is a type II transmembrane glycoprotein inhibitory receptor belonging to the C type lectin-like superfamily. KLRG1 exists both as a monomer and as a disulfide-linked homodimer. This well-conserved receptor is found on the most mature and recently activated NK cells as well as on a subset of effector/memory T cells. Using KLRG1 tetramer as well as other methods, E-, N-, and R-cadherins were identified as KLRG1 ligands. These Ca(2+)-dependent cell-cell adhesion molecules comprises of an extracellular domain containing five cadherin repeats responsible for cell-cell interactions, a transmembrane domain and a cytoplasmic domain that is linked to the actin cytoskeleton. Generation of the KLRG1 tetramer was essential to the identification of the KLRG1 ligands. KLRG1 tetramer is also a unique tool to elucidate the roles cadherin and KLRG1 play in regulating the immune response and tissue integrity.
authors.affiliation: Department of Molecular Microbiology and Immunology, Brown University, USA.
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Terrizzi
authors.forename: Cindy
authors.initials: C
authors.lastname: Banh
authors.forename: Laurent
authors.initials: L
authors.lastname: Brossay
mesh_terms: D015820:Cadherins / Q000737:chemistry; D037181:Lectins, C-Type / Q000737:chemistry* / Q000302:isolation & purification; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D017510:Protein Folding; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D015820:Cadherins; D037181:Lectins, C-Type; D008024:Ligands; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic