title: Cell-seeding techniques in vascular tissue engineering.
abstract: Previous studies have demonstrated the benefits of cell seeding in the construction of tissue-engineered vascular grafts (TEVG). However, seeding methods are diverse and no method is clearly superior in either promoting seeding efficiency or improving long-term graft function. As we head into an era during which a variety of different TEVG are under investigation in clinical trials around the world, it is important to consider the regulatory issues surrounding the translation of these technologies. In this review, we summarize important advances in the field of vascular tissue engineering, with particular attention on cell-seeding techniques for TEVG development and special emphasis placed on regulatory issues concerning the clinical translation of these various methods.
authors.affiliation: Department of Surgery, Yale University School of Medicine, Yale New Haven Hospital, New Haven, Connecticut 06520-8062, USA.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Villalona
authors.forename: Brooks
authors.initials: B
authors.lastname: Udelsman
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Duncan
authors.forename: Edward
authors.initials: E
authors.lastname: McGillicuddy
authors.forename: Rajendra F
authors.initials: RF
authors.lastname: Sawh-Martinez
authors.forename: Narutoshi
authors.initials: N
authors.lastname: Hibino
authors.forename: Christopher
authors.initials: C
authors.lastname: Painter
authors.forename: Tamar
authors.initials: T
authors.lastname: Mirensky
authors.forename: Benjamin
authors.initials: B
authors.lastname: Erickson
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shinoka
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Breuer
mesh_terms: D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D017690:Cell Transplantation / Q000379:methods*; D042783:Endothelial Cells / Q000637:transplantation; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds