Characterization of an activating transcription factor 4 gene containing a consensus phosphorylation site for PKA in the gonads of Xenopus embryos.
Activating transcription factor / cyclic-AMP response element-binding protein (ATF/CREB) has been implicated as a key regulator in the transcriptional control of many genes. In this study, we isolated and characterized a full-length cDNA that encodes a CRE-binding protein 2 (CREB2) called ATF4 in Xenopus embryos. Like other CREB 2 transcription factors, the 342-amino acid ATF4 protein contains a carboxyl terminal leucine-zipper motif, an adjacent basic domain, and an amino terminal leucine-zipper motif. Unlike other CREB2 (ATF4) proteins, the ATF4 isolated from the gonads of Xenopus embryos contains a consensus phosphorylation site for protein kinase A (PKA). In a gel shift analysis, ATF4 bound to a CLS sequence in the promoter of Xenopus aromatase.
Komatsuzaki
Etsuko
E
Laboratory of Biochemistry, Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo, Japan.
Kitamura
Takeaki
T
Murayama
Ikko
I
Saigo
Yuko
Y
Ojima
Kumiko
K
Akatsuka
Naoya
N
Iwabuchi
Junshin
J
Miyata
Shohei
S
D051701:Activating Transcription Factor 4 / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016384:Consensus Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006066:Gonads / Q000196:embryology* / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D016415:Sequence Alignment; D014981:Xenopus / Q000196:embryology*
D051701:Activating Transcription Factor 4; D017868:Cyclic AMP-Dependent Protein Kinases