[Genetic characteristics of the matrix protein of human respiratory syncytial viruses].
OBJECTIVE
Describe the genetic characteristics of Matrix protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.


METHODS
RT-PCR was performed to amplify the M protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in 2004. The RT-PCR products were sequenced for M protein coding region. The sequences of M protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.


RESULTS
The nucleotide acid and deduced amino acid different numbers between 2 A Beijing isolates and prototype strain Long were 24, 26 (3.1%, 3.4%) and 1 (0.39%). The nucleotide acid and deduced amino acid different numbers between 2 B Beijing 2004 isolates and reference strain 9320 were 16, 19 (2.1%, 2.46%) and 2, 1 (0.78%, 0.39%). The nucleotide acid and deduced amino acid different numbers were 24-119 (3.1%-15.4%) and 1-21 (0.39%-8.2%) respectively between 4 Beijing 2004 isolates and GenBank sequences.


CONCLUSIONS
M gene was the highly preserved gene in the HRSV genome. However, numbers of variation occurred in the entire gene of M protein. The variation between A and B subgroups were widely distributed in the entire gene of M protein, while the differences within the A or B subgroups HRSV was pretty low. These sequence data will be the basic gene databank and will be contribute to the development of the quick gene diagnosis assay for HRSV.
Zhang
Yan
Y
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wang
Hui-Ling
HL
Xie
Zheng-De
ZD
D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000235:genetics*
D014763:Viral Matrix Proteins