title: Culture human mesenchymal stem cells with calcium phosphate cement scaffolds for bone repair.
abstract: Because of its moldability and excellent osteoconductivity, calcium phosphate cement (CPC) is highly promising for craniofacial and orthopedic applications. The objectives of this study were to investigate the response of human mesenchymal stem cells (hMSCs) to a high-strength CPC-chitosan scaffold and to examine cell proliferation and osteogenic differentiation. hMSCs were seeded onto CPC-chitosan composite, CPC control, and tissue culture polystyrene (TCPS). Alkaline phosphatase activity (ALP) and mineralization of hMSCs were measured. CPC-chitosan had a flexural strength (mean + or - SD; n = 5) of (19.5 + or - 1.4) MPa, higher than (8.0 + or - 1.4) MPa of CPC control (p < 0.05). The percentage of live hMSCs on CPC-chitosan was (90.5 + or - 1.3)% at 8 days, matching (90.7 + or - 3.8)% of CPC control (p > 0.1). The CPC-chitosan surface area covered by the attached hMSCs increased from (51 + or - 11)% at 1 day to (90 + or - 4)% at 8 days (p < 0.05), matching those of CPC control (p > 0.1). Hence, the CPC strength was significantly increased via chitosan without compromising the hMSC response. At 8 days, there was a significant increase in ALP of cells in osteogenic media (10.99 + or - 0.93) [(mM pNpp/min)/(microg DNA)] versus control media (3.62 + or - 0.40) (p < 0.05). hMSCs in osteogenic media exhibited greater mineralization area of (47.5 + or - 19.7)% compared with (6.1 + or - 2.3)% in control medium on TCPS (p < 0.05). In conclusion, hMSCs showed excellent attachment and viability on the strong and tough CPC-chitosan scaffold, matching the hMSC response on CPC control. hMSCs were successfully differentiated down the osteogenic lineage. Hence, the strong, in situ hardening CPC-chitosan scaffold may be useful as a moderate load-bearing vehicle to deliver hMSCs for maxillofacial and orthopedic bone tissue engineering.
authors.affiliation: Department of Endodontics, Prosthodontics and Operative Dentistry, Dental School, University of Maryland, Baltimore, MD 21201, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Weir
authors.forename: Hockin H K
authors.initials: HH
authors.lastname: Xu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001696:Biomechanical Phenomena; D001843:Bone Cements; D001861:Bone Regeneration; D002130:Calcium Phosphates; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D003470:Culture Media; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D003470:Culture Media; D008903:Minerals; D048271:Chitosan; D000469:Alkaline Phosphatase