Interfacial charge transfer between CdTe quantum dots and gram negative vs gram positive bacteria.
Oxidative toxicity of semiconductor and metal nanomaterials to cells has been well established. However, it may result from many different mechanisms, some requiring direct cell contact and others resulting from the diffusion of reactive species in solution. Published results are contradictory due to differences in particle preparation, bacterial strain, and experimental conditions. It has been recently found that C(60) nanoparticles can cause direct oxidative damage to bacterial proteins and membranes, including causing a loss of cell membrane potential (depolarization). However, this did not correlate with toxicity. In this study we perform a similar analysis using fluorescent CdTe quantum dots, adapting our tools to make use of the particles' fluorescence. We find that two Gram positive strains show direct electron transfer to CdTe, resulting in changes in CdTe fluorescence lifetimes. These two strains also show changes in membrane potential upon nanoparticle binding. Two Gram negative strains do not show these effects-nevertheless, they are over 10-fold more sensitive to CdTe than the Gram positives. We find subtoxic levels of Cd(2+) release from the particles upon irradiation of the particles, but significant production of hydroxyl radicals, suggesting that the latter is a major source of toxicity. These results help establish mechanisms of toxicity and also provide caveats for use of certain reporter dyes with fluorescent nanoparticles which will be of use to anyone performing these assays. The findings also suggest future avenues of inquiry into electron transfer processes between nanomaterials and bacteria.
Dumas
Eve
E
Department of Biomedical Engineering, McGill University, Montreal, QC, Canada H3A 2B4.
Gao
Cherry
C
Suffern
Diana
D
Bradforth
Stephen E
SE
Dimitrijevic
Nada M
NM
Nadeau
Jay L
JL
D002104:Cadmium / Q000633:toxicity*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000378:metabolism*; D053758:Nanoparticles / Q000633:toxicity*; D036103:Nanotechnology; D045663:Quantum Dots; D012666:Semiconductors / Q000009:adverse effects; D013691:Tellurium / Q000633:toxicity*
D002104:Cadmium; D013691:Tellurium