title: The effect of interferon-gamma and lipopolysaccharide on the growth of Francisella tularensis LVS in murine macrophage-like cell line J774.
abstract: BACKGROUND
  Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells.
  
  
  OBJECTIVE
  To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide.
  
  
  RESULTS
  Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection.
  
  
  CONCLUSIONS
  Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis.
authors.affiliation: Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Králové, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Holická
authors.forename: Jakub
authors.initials: J
authors.lastname: Novosad
authors.forename: Martina
authors.initials: M
authors.lastname: Loudová
authors.forename: Manuela
authors.initials: M
authors.lastname: Kudlová
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
mesh_terms: D000818:Animals; D002460:Cell Line; D005604:Francisella tularensis / Q000254:growth & development*; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins
chemical_list: D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007371:Interferon-gamma