Steroids and triterpenes from the fruit bodies of Ganoderma lucidum and their anti-complement activity.
To determine the anti-complement activity of natural triterpenes, chromatographic separation of the EtOAc-soluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of three steroids and five triterpenoids. They were identified as ergosterol peroxide (1), ergosterol (2), genoderic acid Sz (3), stella sterol (4), ganoderic aic C1 (5), ganoderic acid A (6), methyl ganoderate A (7), and lucidenic acid A (8) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Compounds 2 and 3 showed potent anti-complement activity with IC50 values of 52.0 and 44.6 microM, respectively. Compound 1 exhibited significant inhibitory activity with an IC50 value of 126.8 microM, whereas compounds 4-8 were inactive. Our findings suggested that in addition to the ketone group at C-3, the delta7(8), delta9(11)-lanostadiene type triterpene also plays an important role in inhibiting the hemolytic activity of human serum against erythrocytes.
Seo
Hyo Won
HW
College of Pharmacy, Catholic University of Daegu, Gyeongsan, Gyeongbuk 712-702, Korea.
Hung
Tran Manh
TM
Na
MinKyun
M
Jung
Hyun Ju
HJ
Kim
Jin Cheol
JC
Choi
Jae Sue
JS
Kim
Jung Hee
JH
Lee
Hyeong-Kyu
HK
Lee
IkSoo
I
Bae
KiHwan
K
Hattori
Masao
M
Min
Byung Sun
BS
D000818:Animals; D003167:Complement Activation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D048690:Fruiting Bodies, Fungal; D006801:Humans; D020128:Inhibitory Concentration 50; D020940:Reishi / Q000737:chemistry*; D012756:Sheep; D013256:Steroids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
D013256:Steroids; D014315:Triterpenes