Changes in the activities of semicarbazide-sensitive amine oxidase in inferior mesenteric artery segments and in serum of patients with type 2 diabetes.
This study aimed to evaluate the semicarbazide-sensitive amine oxidase (SSAO) activity in human arterial tissues and in serum of patients with type 2 diabetes. The SSAO activity, with (14)C-benzylamine as substrate, was measured in homogenates of human inferior mesenteric arteries obtained at surgery, from 10 patients with type 2 diabetes and 16 non-diabetic patients and in the serum of 39 patients with type 2 diabetes and 40 non-diabetic control patients. The SSAO activity in the homogenates of vascular tissue was significantly lower in the diabetics than in the non-diabetics (P = 0.001). The SSAO activity in the serum of patients with type 2 diabetes was higher when compared with control group (P = 0.0001). In conclusion, the SSAO activity increased in the serum and decreased in the arterial tissue. These findings suggest damage in the vascular tissue and support the hypothesis that serum SSAO may be a useful biochemical marker for diabetes.
Nunes
Sandra Fernanda
SF
Laboratory of Pharmacology, Faculty of Pharmacy, Coimbra University, 3000-548, Coimbra, Portugal.
Figueiredo
Isabel Vitoria
IV
Pereira
João Santos
JS
Soares
Paulo João
PJ
Caramona
Maria Margarida
MM
Callingham
Brian
B
D000368:Aged; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D015179:Colorectal Neoplasms / Q000601:surgery; D003920:Diabetes Mellitus / Q000097:blood / Q000201:enzymology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications / Q000201:enzymology; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008638:Mesenteric Arteries / Q000201:enzymology* / Q000601:surgery; D008875:Middle Aged; D012016:Reference Values
D015415:Biomarkers; D006631:Amine Oxidase (Copper-Containing)