Two-photon microscopy by wavelength-swept pulses delivered through single-mode fiber.
Nonlinear microscopy through flexible fiber-optic catheters has potential in clinical diagnostic applications. Here, we demonstrate a new approach based on wavelength-swept narrowband pulses that permits simple fiber-optic delivery without need of the dispersion management and allows nonmechanical beam scanning. Using 0.86 ps pulses rapidly tuned from 789 nm to 822 nm at a sweep rate of 200 Hz, we demonstrate two-photon fluorescence and second-harmonic generation imaging through a 5-m-long standard single-mode fiber.
Kang
Jeon Woong
JW
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom Street,BAR-8, Boston, Massachusetts 02114, USA.
Kim
Pilhan
P
Alonzo
Carlo Amadeo
CA
Park
Hyunsung
H
Yun
Seok H
SH
D000818:Animals; D005240:Feasibility Studies; D007834:Lasers; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism; D055595:Mechanical Phenomena; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D055100:Optical Fibers; D017785:Photons; D012235:Rhodamines / Q000378:metabolism; D013623:Tail; D013710:Tendons / Q000166:cytology; D013997:Time Factors
D012235:Rhodamines; C029773:rhodamine B