A new DNA combing method for biochemical analysis.
A simple molecular combing method for analysis of biochemical reactions, called the moving droplet method, has been developed. In this method, small droplets containing DNA molecules run down a sloped glass substrate, and this creates a moving interface among the air, droplet, and substrate that stretches the DNA molecules. This method requires a much smaller volume of sample solution than other established combing methods, allowing wider application in various fields. Using this method, lambdaDNA molecules were stretched and absorbed to a glass substrate, and single-molecule analysis of DNA synthesis by DNA polymerases was performed.
Oshige
Masahiko
M
Department of Chemical and Environmental Engineering, Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan.
Yamaguchi
Kouji
K
Matsuura
Shun-Ichi
S
Kurita
Hirofumi
H
Mizuno
Akira
A
Katsura
Shinji
S
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D005898:Glass; D051381:Rats
D002232:Carbocyanines; D004279:DNA, Viral; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D004259:DNA-Directed DNA Polymerase