Free radical scavenging activity and antiproliferative potential of Polygonum cuspidatum root extracts.
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, ethanol and ethyl acetate extracts of P. cuspidatum root were assayed for their 1,1-diphenyl-2-hydrazyl (DPPH) and hydroxyl free radical scavenging activities, total phenolics content, protective effect against DNA damage, and antiproliferative activity on human lung cancer cells. The ethanol and ethyl acetate (lipophilic phase) extracts of P. cuspidatum had significant scavenging effects on DPPH and hydroxyl radicals. Total phenolics content of ethanol and ethyl acetate (lipophilic phase) extracts of P. cuspidatum were 276.78 +/- 39.31 and 231.73 +/- 5.04 mg/ml, respectively; both extracts protected against hydroxyl radical-induced DNA strand scission. Furthermore, the extracts of P. cuspidatum induced apoptosis and inhibited cell growth in A549 and H1650 cell lines, suggesting that P. cuspidatum root extracts exhibit an antiproliferative effect on human lung cancer cells.
Lin
Yun-Wei
YW
Department of Biochemical Science and Technology, National Chiayi University, 300 University Road, Chiayi, Taiwan, ROC.
Yang
Fu-Jung
FJ
Chen
Chia-Ling
CL
Lee
Wei-Ting
WT
Chen
Ruey-Shyang
RS
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D045468:Fallopia japonica; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D014407:Tumor Cells, Cultured
D016166:Free Radical Scavengers; D006131:Growth Inhibitors; D010936:Plant Extracts