title: A new thermally immobilized fluorinated stationary phase for RP-HPLC.
abstract: A new fluorinated stationary phase was prepared through thermal immobilization of poly(methyl-3,3,3-trifluoropropylsiloxane) onto 5 microm Kromasil silica particles. The best conditions of immobilization time and temperature were determined through a central composite design and response surface methodologies. Physical-chemical characterization using solid-state (29)Si NMR measurements, infrared spectroscopy and elemental analysis showed that the immobilization process was effective to promote a coating of the support that corresponds to a monolayer of polymer. The stationary phase presents selectivity for positional isomers and good peak shape for basic compounds.
authors.affiliation: Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Liane
authors.initials: L
authors.lastname: Maldaner
authors.forename: Isabel C S F
authors.initials: IC
authors.lastname: Jardim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006358:Hot Temperature; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D009930:Organic Chemicals / Q000032:analysis / Q000302:isolation & purification; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013818:Thermogravimetry
chemical_list: D004129:Dimethylpolysiloxanes; D009930:Organic Chemicals; D012997:Solvents; C049525:polymethyl-3,3,3-trifluoropropylsiloxane; D012822:Silicon Dioxide