Protective effects of antioxidant combination against D-galactosamine-induced kidney injury in rats.
The protective effects of an antioxidant combination in kidney injury induced by the injection of D-galactosamine (D-GaIN) were examined in the present study. Sprague Dawley female rats were used and divided into four groups as follows: (1) animals injected physiological saline solution, intraperitoneally, (2) animals treated with the combination of ascorbic acid (100 mg kg(-1) day(-1)), beta-carotene (15 mg kg(-1) day(-1)), alpha-tocopherol (100 mg kg(-1) day(-1)), and sodium selenate (0.2 mg kg(-1) day(-1)) for three days orally, (3) rats injected D-GaIN (500 mg kg(-1)) intraperitoneally as a single dose, and (4) animals treated with the antioxidant combination for three days, then injected D-GaIN. The tissue and blood samples of animals were collected for morphological and biochemical evaluations. Histopathological injury in kidney tissues was observed together with a significant increase in tissue lipid peroxidation (LPO) level, myeloperoxidase (MPO), lactate dehydrogenase, catalase and superoxide dismutase (SOD) activities, and serum creatinine and urea levels, and a significant decrease in glutathione level and glutathione peroxidase activity in D-GaIN injected rats. However, a decrease in the degenerative changes was detected in the kidney tissue of D-GaIN + antioxidant group, and biochemical results showed reversed effects. In conclusion, it seems reasonable to conclude that the treatment of the antioxidant combination has a protective effect on D-GaIN-induced kidney injury of rats.
Catal
Tunc
T
Department of Biology, Istanbul University, Vezneciler, Turkey.
Sacan
Ozlem
O
Yanardag
Refiye
R
Bolkent
Sehnaz
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D003404:Creatinine / Q000097:blood; D005260:Female; D005688:Galactosamine / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D045022:Lactate Dehydrogenases / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064586:Selenic Acid; D018036:Selenium Compounds / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000097:blood; D024502:alpha-Tocopherol / Q000494:pharmacology; D019207:beta Carotene / Q000494:pharmacology
D000975:Antioxidants; D018036:Selenium Compounds; D019207:beta Carotene; D005688:Galactosamine; D014508:Urea; D003404:Creatinine; D045022:Lactate Dehydrogenases; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D024502:alpha-Tocopherol; D064586:Selenic Acid; D001205:Ascorbic Acid