Ror2 is required for midgut elongation during mouse development.
The receptor tyrosine kinase Ror2 acts as a receptor for Wnt5a to mediate noncanonical Wnt signaling, and it plays essential roles in morphogenesis. Ror2-/- embryos exhibit phenotypes similar to, albeit generally milder than, those of Wnt5a-/- embryos. During mouse embryogenesis, Ror2 is expressed in various organs and regions, although little is known about its expression pattern and roles in the developing gut, while Wnt5a is expressed in the developing gut, where its absence causes abnormal phenotypes. Here, we demonstrated that Ror2 was strongly and differentially expressed in the rostral and middle midgut endoderm from embryonic day (E) 10.5 through embryonic day (E) 12.5. At E11.5, Ror2-/- embryos exhibited a shorter middle midgut with a larger diameter and more accumulation of epithelial cells in the middle midgut than control embryos, while the total cell numbers remained unaltered. These findings suggest that Ror2 plays important roles in midgut elongation by means of an epithelial convergent extension mechanism.
Yamada
Makiko
M
Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Japan. makiyamd@med.shimane-u.ac.jp
Udagawa
Jun
J
Matsumoto
Akihiro
A
Hashimoto
Ryuju
R
Hatta
Toshihisa
T
Nishita
Michiru
M
Minami
Yasuhiro
Y
Otani
Hiroki
H
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D016764:Cell Polarity; D004847:Epithelial Cells / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007422:Intestines / Q000196:embryology*; D008024:Ligands; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000378:metabolism*; D013997:Time Factors; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein
D008024:Ligands; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D001973:Bromodeoxyuridine