Co-expression of alpha' and beta subunits of beta-conglycinin in rice seeds and its effect on the accumulation behavior of the expressed proteins.
A transgenic rice that produces both the alpha' and beta subunits of beta-conglycinin has been developed through the crossing of two types of transgenic rice. Although the accumulation level of the alpha' subunit in the alpha'beta-transgenic rice was slightly lower than that in the transgenic rice producing only the alpha' subunit, the accumulation level of the beta subunit in the alpha'beta-transgenic rice was about 60% higher than that in the transgenic rice producing only the beta subunit. Results from sequential extraction and gel-filtration experiments indicated that part of the beta subunit formed heterotrimers with the alpha' subunit in a similar manner as in soybean seeds and that the heterotrimers interacted with glutelin via cysteine residues. These results imply that the accumulation level of the beta subunit in the alpha'beta-transgenic rice increases by an indirect interaction with glutelin. Immunoelectron microscopy revealed that the alpha' and beta subunits are localized in a low electron-dense region of protein body-II (PB-II) and that alpha' homotrimers in the alpha'beta-transgenic rice seeds seem to accumulate outside of this low electron-dense region.
Motoyama
Takayasu
T
Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyou, Kyoto, Japan.
Okumoto
Yutaka
Y
Tanisaka
Takatoshi
T
Utsumi
Shigeru
S
Maruyama
Nobuyuki
N
D052179:Antigens, Plant / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D003433:Crosses, Genetic; D003545:Cysteine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D005916:Globulins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D005983:Glutens / Q000737:chemistry; D016253:Microscopy, Immunoelectron; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D025941:Protein Interaction Mapping; D055503:Protein Multimerization; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D055314:Seed Storage Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000378:metabolism; D030262:Soybean Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D019076:Transgenes
D052179:Antigens, Plant; D005916:Globulins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D005983:Glutens; D003545:Cysteine