Parallel, tag-directed assembly of locally derived short sequence reads.
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
Hiatt
Joseph B
JB
Department of Genome Sciences, University of Washington, Seattle, Washington, USA. jbhiatt@u.washington.edu
Patwardhan
Rupali P
RP
Turner
Emily H
EH
Lee
Choli
C
Shendure
Jay
J
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D020224:Expressed Sequence Tags; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*