An exposure assessment of di-(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) in human semen.
Levels of the phthalates such as di(2-ethylhexyl) phthalate (DEHP), mono(2-ethylhexyl) phthalate (MEHP, a major metabolite of DEHP), di-n-butyl phthalate (DBP), mono-n-butyl phthalate (MBP, a major metabolite of DBP), and phthalic acid (P, (a common metabolite of phthalates, including DEHP and DBP) were determined in the semen samples of 99 healthy volunteers without known prior medicosurgical history. Samples were obtained from young men (age 20-25 yr) who visited a clinic, and the semen concentrations of phthalates were measured using ultra-performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS). UPLC/MS/MS showed that mean concentrations in semen samples were 1.07 microg/ml for MEHP, 0.61 microg/ml for DEHP, 0.39 microg/ml for PA, 0.06 microg/ml for MBP, and 0.003 microg/ml for DBP. The concentration of MEHP (the metabolite of DEHP) was highest, and the concentrations of the metabolites including MEHP, MBP, and PA were higher than actual concentrations of parent DEHP and DBP. These findings suggest the detection of phthalates in healthy human semen might require further investigation for effects on human fertility.
Han
Sang Won
SW
Department of Urology, Urological Science Institute, Yonsei University College of Medicine, Seoul, South Korea.
Lee
Hyeyoung
H
Han
Soon Young
SY
Lim
Duck Soo
DS
Jung
Ki Kyung
KK
Kwack
Seung Jun
SJ
Kim
Kyu Bong
KB
Lee
Byung Mu
BM
D003993:Dibutyl Phthalate / Q000032:analysis*; D004051:Diethylhexyl Phthalate / Q000032:analysis*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000032:analysis; D006801:Humans; D008297:Male; D010968:Plasticizers / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D055815:Young Adult
D004785:Environmental Pollutants; D010968:Plasticizers; D003993:Dibutyl Phthalate; D004051:Diethylhexyl Phthalate