title: Cyanobacterial bioreporters as sensors of nutrient availability.
abstract: Due to their ubiquity in aquatic environments and their contribution to total biomass, especially in oligotrophic systems, cyanobacteria can be viewed as a proxy for primary productivity in both marine and fresh waters. In this chapter we describe the development and use of picocyanobacterial bioreporters to measure the bioavailability of nutrients that may constrain total photosynthesis in both lacustrine and marine systems. Issues pertaining to bioreporter construction, performance and field applications are discussed. Specifically, luminescent Synechococcus spp. and Synechocystis spp. bioreporters are described that allow the bioavailability of phosphorus, nitrogen and iron to be accurately measured in environmental samples.
authors.affiliation: Department of Biological Sciences, Bowling Green State University, Bowling Green, OH, 43403, USA.
authors.forename: George S
authors.initials: GS
authors.lastname: Bullerjahn
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Boyanapalli
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Rozmarynowycz
authors.forename: R Michael L
authors.initials: RM
authors.lastname: McKay
mesh_terms: D001682:Biological Availability; D015374:Biosensing Techniques / Q000379:methods*; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004777:Environment; D017930:Genes, Reporter / Q000235:genetics