Transfer of the beta-tubulin gene of Botrytis cinerea with resistance to carbendazim into Fusarium graminearum.
BACKGROUND
Resistance to carbendazim and other benzimidazole fungicides in Botrytis cinerea (Pers. ex Fr.) and most other fungi is usually conferred by mutation(s) in a single chromosomal beta-tubulin gene, often with several allelic mutations. In Fusarium graminearum Schwade, however, carbendazim resistance is not associated with a mutation in the corresponding beta-tubulin gene.


RESULTS
The beta-tubulin gene conferring carbendazim resistance in B. cinerea was cloned and connected with two homologous arms of the beta-tubulin gene of F. graminearum by using a double-joint polymerase chain reaction (PCR). This fragment was transferred into F. graminearum via homologous double crossover at the site where the beta-tubulin gene of F. graminearum is normally located (the beta-tubulin gene of F. graminearum had been deleted). The transformants were confirmed and tested for their sensitivity to carbendazim.


CONCLUSIONS
The beta-tubulin gene conferring carbendazim resistance in B. cinerea could not express this resistance in F. graminearum, as transformants were still very sensitive to carbendazim.
Liu
Sheng-Ming
SM
College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China.
Chen
Yu
Y
Yu
Jun-Jie
JJ
Chen
Chang-Jun
CJ
Wang
Jian-Xin
JX
Zhou
Ming-Guo
MG
D001562:Benzimidazoles / Q000633:toxicity*; D020171:Botrytis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D002219:Carbamates / Q000633:toxicity*; D004351:Drug Resistance / Q000235:genetics*; D005670:Fusarium / Q000235:genetics*; D018014:Gene Transfer Techniques; D020128:Inhibitory Concentration 50; D017354:Point Mutation; D014404:Tubulin / Q000235:genetics*
D001562:Benzimidazoles; D002219:Carbamates; D014404:Tubulin; C006698:carbendazim