title: Polymorphism of the bovine ADRB3 gene.
abstract: The β(3)-adrenergic receptor (ADRB3) is predominantly expressed in white and brown adipose tissue and mediates the lipolytic and thermogenic effects of high catecholamine concentrations. Variation in the ADRB3 gene (ADRB3) has been associated with obesity and the earlier onset of non-insulin-dependent diabetes mellitus in some ethnic groups, as well as some production traits of sheep, but to date variation of bovine ADRB3 has not been reported. In this study, variation in the promoter region of bovine ADRB3 was investigated in 737 cattle by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. Six PCR-SSCP patterns representing six allelic variations and containing four single nucleotide polymorphisms (SNPs) and three nucleotide deletions/insertions were observed. Allele A was the most common allele (93.83%), whereas alleles C, D, E and F were rare (0.07, 1.09, 0.41, and 0.34%, respectively). The variation identified here might have an impact on both the function and level of expression of bovine ADRB3.
authors.affiliation: Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Huitong
authors.initials: H
authors.lastname: Zhou
authors.forename: Anna
authors.initials: A
authors.lastname: Smyth
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Luo
authors.forename: Jon G H
authors.initials: JG
authors.lastname: Hickford
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D022702:Receptors, Adrenergic, beta-3