title: Genetic verification of Bordetella pertussis seed strains used for production of Japanese acellular pertussis vaccines.
abstract: In Japan, the Bordetella pertussis strain Tohama provided by the National Institute of Health, Japan has been used for the production of acellular pertussis (aP) vaccines since 1981. In the present study, in order to verify the genetic consistency of B. pertussis vaccine seed strains, we analyzed the genetic properties of the working seeds obtained from five Japanese vaccine manufacturers, and compared them with those of B. pertussis Tohama reference strains (NIID L-7 and ATCC BAA-589). Genetic analyses with pulsed-field gel electrophoresis and allele typing showed 100% genetic identity among the five seed strains and the Tohama reference strains. In addition, Southern blot analyses revealed the absence of four orthologous genes (BB0537, BB0920, BB1149 and BB4885), which are specifically absent in the strain Tohama, and in the genome of all seed strains tested, suggesting that the regions of difference (RD11-RD14) are absent in their genomes. Consequently, no genetic difference was observed among the working seeds and Tohama reference strains. Our observations indicate that B. pertussis seed strains for Japanese aP vaccine production are genetically comparable with B. pertussis Tohama.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan. kamachi@nih.go.jp
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kamachi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fukuda
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Han
authors.forename: Hiromi
authors.initials: H
authors.lastname: Toyoizumi-Ajisaka
authors.forename: Keiko
authors.initials: K
authors.lastname: Mochida
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Konda
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques; D001885:Bordetella Infections / Q000276:immunology / Q000517:prevention & control*; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D016680:Genome, Bacterial; D006801:Humans; D007564:Japan; D010567:Pertussis Vaccine / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D022282:Vaccines, Acellular / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D004269:DNA, Bacterial; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular