title: Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection.
abstract: A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 94 degrees C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity (R(2)>0.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps, which provides another view for quality control of Cordyceps sinensis.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Macao SAR, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Feng-Qing
authors.initials: FQ
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Feng
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D008027:Light; D009705:Nucleosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D012542:Scattering, Radiation
chemical_list: D002241:Carbohydrates; D004365:Drugs, Chinese Herbal; D005229:Fatty Acids, Monounsaturated; D009705:Nucleosides; C001996:thermozymocidin