[Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress].
OBJECTIVE
To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.


METHODS
BALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.


RESULTS
In control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).


CONCLUSIONS
The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.
Xu
Ya-juan
YJ
Deparment of Prosthodontics, Sun Yat-sen University, Guangzhou 510055, China.
Zhang
Yi-ping
YP
Qin
Feng
F
Wu
Chang-jing
CJ
Li
You-rui
YR
Fu
Qiang
Q
D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012212:Rheology; D013314:Stress, Mechanical
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015638:Cytochalasin D