title: Nisin affects the growth of Listeria monocytogenes on ready-to-eat turkey ham stored at four degrees Celsius for sixty-three days.
abstract: The objectives of this study were to determine pH, anti-Listeria and general antimicrobial properties of nisin on ready-to-eat vacuum-packaged diced turkey ham inoculated with Listeria monocytogenes, and the usage level that would exert maximum antimicrobial effect during 63 d of storage. Ready-to-eat diced turkey ham was inoculated with a 5-strain L. monocytogenes cocktail; treated with 0.2, 0.3, 0.4, and 0.5% nisin treatment solutions; vacuum-packaged; stored at 4+/-1 degrees C for 63 d; and analyzed at 1-wk intervals for total aerobic counts, pH, L. monocytogenes, and lactic acid bacteria. Antimicrobial effectiveness of nisin increased as concentration increased from 0.2 to 0.5%. Aerobic plate counts for 0.4 and 0.5% nisin were lower (P<0.05) than negative and positive controls. All nisin treatments resulted in 4 log reductions (P<0.05) in L. monocytogenes when compared with the positive control on d 0. Four log reductions were also observed on d 7 for 0.4% nisin treatment and d 7 and 14 for 0.5% nisin treatment when compared with the positive control. Listeria monocytogenes counts decreased from 4.97 log cfu/g on d 0 and remained less than 2 log cfu/g through 63 d of storage for the 0.5% nisin treatment. Lactic acid bacteria counts were lower (P<0.05) for 0.5% nisin treatment when compared with positive and negative controls from 28 through 63 d. Except for d 56 and 63, pH was similar (P<0.05) for all treatments. This study revealed that nisin could be used for postprocessing intervention to control L. monocytogenes in ready-to-eat poultry products.
authors.affiliation: Department of Animal Sciences, PO Box 110910, University of Florida, Gainesville 32611-0910, USA.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz
authors.forename: S K
authors.initials: SK
authors.lastname: Williams
authors.forename: N
authors.initials: N
authors.lastname: Djeri
authors.forename: A
authors.initials: A
authors.lastname: Hinton
authors.forename: G E
authors.initials: GE
authors.lastname: Rodrick
mesh_terms: D000818:Animals; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008461:Meat Products / Q000382:microbiology*; D009561:Nisin / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014422:Turkeys / Q000382:microbiology*
chemical_list: D005520:Food Preservatives; D009561:Nisin