[Detection and clinical implication of up-regulated gene 1 mRNA levels with real-time quantitative RT-PCR in chronic lymphocytic leukemia cells.].
OBJECTIVE
To investigate the expression level of chronic lymphocytic leukemia (CLL) up-regulated gene 1 (CLLU1) mRNA in CLL and its prognostic value in CLL.


METHODS
Real-time quantitative RT-PCR (qRT-PCR) was performed on 41 CLL patients using Taqman probe system. Correlation of CLLU1 expression ratio with other prognostic factors was carried out using Spearman correlation coefficient.


RESULTS
The correlation coefficients of the standard curves in qRT-PCR were above 0.99. The coefficients of variation (CV) of interrun assay and intrarun assay were < 5% and the sensitivity reached 10(2) copies/microg RNA. The median CLLU1 mRNA expression level was 0.139 (0 - 5256.912) in 41 CLL patients. CLLU1 expression was significantly associated with Binet stages (P = 0.040) and IgVH mutation status (P = 0.021). CLLU1 expression was also associated with CD(38) expression (P = 0.045).


CONCLUSIONS
qRT-PCR assay is reliable and sensitive. CLLU1 mRNA expression significantly correlates with clinical stages, IgVH mutation status and CD(38) expression and might be a prognostic maker of CLL.
Gu
Wei-Jun
WJ
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
Xu
Wei
W
Miao
Kou-Rong
KR
Zhu
Dan-Xia
DX
Qiao
Chun
C
Li
Jian-Yong
JY
D051997:ADP-ribosyl Cyclase 1; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051746:ZAP-70 Protein-Tyrosine Kinase
D012333:RNA, Messenger; D051746:ZAP-70 Protein-Tyrosine Kinase; D051997:ADP-ribosyl Cyclase 1