Estimation of combined effects of carbon and nitrogen sources on the growth and bacteriocin production of Lactobacillus salivarius from human source.
Vaginal Lactobacillus salivarius CRL 1328 (Centro de Referencia para Lactobacilos Culture Collection) or the bacteriocin that produces could be included in a urogenital probiotic formula to prevent urogenital infections in women. The objective of this work was to determine the effects of different carbon and nitrogen sources on the growth and bacteriocin production of this microorganism. A fractional factorial design 3(5-1) was applied to evaluate the effects of five nutrients (glucose, lactose, yeast extract, tryptone and meat peptone), at three different concentrations (0, 1 and 2%). Results were statistically analyzed for linear and quadratic effects of nutrients, along with their interactions. All the nutrients tested stimulated the cell growth and bacteriocin production, but lactose had not a significant influence on the last response. The linear effects of higher magnitude on biomass and bacteriocin production were those of yeast extract and tryptone. The results demonstrated that there were significant interactions between the different nutrients, depending on the response evaluated. Maximum bacteriocin production was reached in different growth media with a lower cost than conventional culture media used in the laboratory. These findings will contribute to the design of a pharmaceutical product for the restoration of ecological balance of urogenital tract.
Tomás
María Silvina Juárez
MS
Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco, Tucumán, Argentina.
Bru
Elena
E
Nader-Macías
María Elena Fátima
ME
D001430:Bacteriocins / Q000096:biosynthesis*; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D014621:Vagina / Q000382:microbiology
D001430:Bacteriocins; D003470:Culture Media; D002244:Carbon; D009584:Nitrogen