Reinekea aestuarii sp. nov., isolated from tidal flat sediment.
Two Gram-negative, non-motile, non-pigmented and curved rod-shaped bacterial strains, designated IMCC4489(T) and IMCC4451, were isolated from a tidal flat sediment of the Yellow Sea. Strains IMCC4489(T) and IMCC4451 shared 99.9 % 16S rRNA gene sequence similarity and 78.5 % DNA-DNA relatedness, which suggested that they belonged to the same species. The isolates were most closely related to Reinekea blandensis MED297(T) (98.7-98.8 % 16S rRNA gene sequence similarity) and Reinekea marinisedimentorum DSM 15388(T) (95.3-95.4 %). DNA-DNA relatedness between the strains and R. blandensis CCUG 52066(T) was 31-34 %. Strains IMCC4489(T) and IMCC4451 could also be differentiated from the type strains of the two recognized Reinekea species by several phenotypic properties. The DNA G+C content was 51.3-51.5&emsp14;mol% and the major isoprenoid quinone was Q-8. On the basis of the data obtained in this study, it is proposed that strains IMCC4489(T) and IMCC4451 represent a novel species, Reinekea aestuarii sp. nov. The type strain is IMCC4489(T) (=KCTC 22813(T) =KCCM 42938(T) =NBRC 106079(T)).
Choi
Ahyoung
A
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Cho
Jang-Cheon
JC
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D044130:Oceanospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009792:Oceans and Seas; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8