title: [Effect of shuizhongcao granule on cellular immune function of experimental animal with recurrent aphthous stomatitis].
abstract: OBJECTIVE
  To investigate the effect of Shuizhongcao Granule (SZCG) on cellular immune function in animal model of recurrent aphthous stomatitis (RAS).
  
  
  METHODS
  Experimental animal model of RAS were established by immunological method. The modeled animals were randomized into 4 groups, Group A was the model control group, Group B was treated by Levamisole (2.5 mg/mL), Group C and D was treated with high-dose (0.5 g/mL) and low-dose (0.24 g/mL) SZCG respectively. The medication was administered via gastric perfusion, starting from the 7th week of the experiment, and continued for 28 days. Meanwhile, a normal control group was set up. Percentage of T-lymphocyte subsets, CD3, CD4 and CD8, and serum levels of interleukin 10 and 12 (IL-10 and IL-12) in peripheral blood were detected before and after treatment.
  
  
  RESULTS
  Percentages of CD3, CD4 and CD4/CD8 ratio in the model control group were 40.50 +/- 7.46%, 30.80 +/- 5.33% and 79.56 +/- 8.32 respectively, all lower than those in the normal control group (P<0.05); level of serum IL-10 was higher (8.02 +/- 0.53 ng/L) and that of IL-12 was lower (15.51 +/- 1.35 ng/L) in the model animal than those in normal control (P<0.05). After treatment, levels of CD3, CD4, CD4/CD8 and IL-12 in Group B were 55.30 +/- 6.33%, 43.50 +/- 5.33%, 99.29 +/- 13.84 and 17.72 +/- 1.70 ng/L, those in Group C were 61.50 +/- 4.32%, 47.20 +/- 4.37%, 103.30 +/- 7.42 and 18.18 +/- 1.54 ng/L, and in Group D were 58.50 +/- 6.03%, 42.80 +/- 3.17%, 110.27 +/- 12.85 and 17.74 +/- 1.96 ng/L, respectively, all were higher than those in Group A, but levels of IL-10 in the three treatment groups were lower than that in Goup A (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  The immune function of RAS model animals is in the suppressed condition; SZCG can improve the immune suppression to achieve its therapeutic effect on the disease.
authors.affiliation: Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, China. smh3288@yahoo.com.cn
authors.forename: Min-He
authors.initials: MH
authors.lastname: Shen
authors.forename: Shan-Ming
authors.initials: SM
authors.lastname: Ruan
authors.forename: Min-Huan
authors.initials: MH
authors.lastname: Bao
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D008297:Male; D009061:Mouth Mucosa; D008517:Phytotherapy; D011817:Rabbits; D013281:Stomatitis, Aphthous / Q000188:drug therapy* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D004365:Drugs, Chinese Herbal; D016753:Interleukin-10; D018664:Interleukin-12