Simultaneous determination of organophosphorus pesticides in Chinese herbal medicines by microwave-assisted extraction coupled with dispersive-solid phase extraction and gas chromatography.
A reliable, rapid and nontoxic analytical method was proposed for the simultaneous determination of 16 organophosphorus pesticides in Chinese herbal medicines. The pesticides were extracted by ethanol and the experimental variables, such as temperature, extraction time and volume of ethanol, were optimized through orthogonal array experimental design. Cleanup of extracts was performed with dispersive-solid phase extraction using primary secondary amine as the sorbent. The determination of pesticides in the final extracts was carried out by gas chromatography-flame photometric detection. Under optimized conditions the obtained recoveries, except for isocarbophos, were in the range 73.8-123%, with relative standard deviations equal to or lower than 15.2% and limits of detection ranging from 0.001 to 0.009 mg/kg.
Wan
Yi-Qun
YQ
Nanchang University, China. yqwanoy@sina.com
Mao
Xue-Jin
XJ
Yan
Ai-Ping
AP
Shen
Ming-Yue
MY
Wu
Yu-Mei
YM
D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008872:Microwaves; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*
D004365:Drugs, Chinese Herbal; D009943:Organophosphorus Compounds; D010575:Pesticides