Light-mediated spatial control via photolabile fluorescently quenched peptide cassettes.
Light-regulatable compounds are finding increasing utility as spatial and temporal probes of biological behavior. An independent measure of successful light-induced structural change is possible when alteration (e.g., activation, deactivation, etc.) of the bioprobe can be directly linked to a fluorescent readout. We have identified a series of photolabile fluorescently quenched cassettes that display extraordinarily large fluorescence enhancements upon photolysis. A pair of cassettes has been inserted into mitochondrial localization sequences to assess an organelle-targeted light-mediated release strategy for controlling biological activity. The peptide constructs are readily absorbed by mitochondria and subsequently can be cleaved in a light-dependent fashion as assessed by the predicted changes in absorbance and fluorescence.
Lee
Hsien-Ming
HM
Department of Chemistry, Division of Medicinal Chemistry and Natural Products, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Priestman
Melanie A
MA
Lawrence
David S
DS
D000595:Amino Acid Sequence; D005456:Fluorescent Dyes / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008928:Mitochondria / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis*; D010782:Photolysis; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D010455:Peptides