title: Haemophilus ducreyi SapA contributes to cathelicidin resistance and virulence in humans.
abstract: Haemophilus ducreyi is an extracellular pathogen of human epithelial surfaces that resists human antimicrobial peptides (APs). The organism's genome contains homologs of genes sensitive to antimicrobial peptides (sap operon) in nontypeable Haemophilus influenzae. In this study, we characterized the sap-containing loci of H. ducreyi 35000HP and demonstrated that sapA is expressed in broth cultures and H. ducreyi-infected tissue; sapA is also conserved among both class I and class II H. ducreyi strains. We constructed a nonpolar sapA mutant of H. ducreyi 35000HP, designated 35000HPsapA, and compared the percent survival of wild-type 35000HP and 35000HPsapA exposed to several human APs, including alpha-defensins, beta-defensins, and the cathelicidin LL-37. Unlike an H. influenzae sapA mutant, strain 35000HPsapA was not more susceptible to defensins than strain 35000HP was. However, we observed a significant decrease in the survival of strain 35000HPsapA after exposure to LL-37, which was complemented by introducing sapA in trans. Thus, the Sap transporter plays a role in resistance of H. ducreyi to LL-37. We next compared mutant strain 35000HPsapA with strain 35000HP for their ability to cause disease in human volunteers. Although both strains caused papules to form at similar rates, the pustule formation rate at sites inoculated with 35000HPsapA was significantly lower than that of sites inoculated with 35000HP (33.3% versus 66.7%; P = 0.007). Together, these data establish that SapA acts as a virulence factor and as one mechanism for H. ducreyi to resist killing by antimicrobial peptides. To our knowledge, this is the first demonstration that an antimicrobial peptide resistance mechanism contributes to bacterial virulence in humans.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Dr., Room MS 420, Indianapolis, IN 46202-5124, USA.
authors.forename: Kristy L B
authors.initials: KL
authors.lastname: Mount
authors.forename: Carisa A
authors.initials: CA
authors.lastname: Townsend
authors.forename: Sherri D
authors.initials: SD
authors.lastname: Rinker
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Gu
authors.forename: Kate R
authors.initials: KR
authors.lastname: Fortney
authors.forename: Beth W
authors.initials: BW
authors.lastname: Zwickl
authors.forename: Diane M
authors.initials: DM
authors.lastname: Janowicz
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Spinola
authors.forename: Barry P
authors.initials: BP
authors.lastname: Katz
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Bauer
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002602:Chancroid / Q000382:microbiology / Q000473:pathology; D017124:Conserved Sequence; D024881:Drug Resistance, Bacterial; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D006191:Haemophilus ducreyi / Q000187:drug effects* / Q000472:pathogenicity*; D006805:Human Experimentation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008875:Middle Aged; D012867:Skin / Q000473:pathology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*; D054804:Cathelicidins
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; C548036:SapA protein, Haemophilus ducreyi; D037521:Virulence Factors; D054804:Cathelicidins