title: [Nitric oxide synthase (NOS) expression and nitric oxide (NO) content in the skeletal muscles in transverse process syndrome of the 3rd lumbar vertebra of model rats treated with acupotomology therapy].
abstract: OBJECTIVE
  To study the relation between the nitric-oxide synthase (NOS) expression and nitric oxide (NO) content in the skeletal muscles and the injury condition of soft tissue in the 3rd lumbar vertebrae syndrome model rats, and to observe the effect of acupotomology therapy.
  
  
  METHODS
  One hundred and twenty-eight adult SD rats were allocated to 4 groups randomly: normal group, model group, aminoguanidin group and acupotomology treatment group, 32 rats in each group. NOS expression, NO content and injury of the soft tissue in the 3rd lumbar vertebra were observed on the 1st, 3rd, 7th and 14th day after the acupotomology treatment and aminoguanidine intervention.
  
  
  RESULTS
  1) Inducible NOS (iNos) activity and NO content in model group was significantly higher (F = 522.860, P < 0.01), in acupotomology group and aminoguanidine group was significantly lower than the model group (FiNOS = 28.894, P < 0.01), and iNOS activity and NO content in all groups was in competence with the condition of soft tissue injuries. 2) Endothelium NOS (eNOS) expression raised in model group and acupotomology group, and achieve peak on the 7th day. There was significant difference between the eNOS expression in acupotomology group and the model group (FeNOS = 3.454, P < 0.05). 3) The expression of neuron NOS (nNOS) in the model group, aminoguanidine group and acupotomology group had no significant (FnNOS = 0.962, P > 0.05).
  
  
  CONCLUSIONS
  Acupotomology treatment can restrain the development of high content NO, release the inflammatory reaction and injury condition, improve microcirculation, prevent the development of scar tissue of the injured soft tissue, and has significant recovering effectiveness in the soft tissue injured model rats.
authors.affiliation: Department of Rehabilitation and Physiotherapy, Navy General Hospital of PLA, Beijing 100037, China.
authors.forename: Jin-Niu
authors.initials: JN
authors.lastname: Li
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Qiao
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Guo
authors.forename: Guang-Cheng
authors.initials: GC
authors.lastname: Ji
authors.forename: Guang-Hao
authors.initials: GH
authors.lastname: Ma
authors.forename: Ben-Sheng
authors.initials: BS
authors.lastname: Fu
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Xiang
authors.forename: Yi-Ying
authors.initials: YY
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Can-Kun
authors.initials: CK
authors.lastname: Liu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D006146:Guanidines / Q000627:therapeutic use; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000601:surgery*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013577:Syndrome; D013997:Time Factors
chemical_list: D006146:Guanidines; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; C004479:pimagedine