Nuclear proteome analysis of monkey embryonic stem cells during differentiation.
The nuclear proteome enables, manages, and regulates the genome by the collective actions and interactions of proteins found in the nucleus. We applied a proteomic approach to analyze a nuclear proteome during embryonic stem cell (ESC) proliferation, and 3 and 9 days after initiation of differentiation. The nuclei were isolated from cells and their proteins were separated using 2-DE. Out of about 560 protein spots reproducible detected on any give gel, 49 differentially expressed proteins were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF/TOF) mass spectrometry. Of them, several nuclear located proteins involved in chromatin remodeling, transcription regulation, apoptosis, cell proliferation, and differentiation were identified including CTBP1, MM-1, RUVBL1, HCC-1, SGTA, SUMO2, and Galectin-1. Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53.
Nasrabadi
Davood
D
Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box: 19395-4644, Karaj, Iran.
Larijani
Mehran Rezaei
MR
Fathi
Ali
A
Gourabi
Hamid
H
Dizaj
Ahmad V
AV
Baharvand
Hossein
H
Salekdeh
Ghasem Hosseini
GH
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D008252:Macaca fascicularis; D009687:Nuclear Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D009687:Nuclear Proteins