Comparison of laboratory colonies and field populations of Tamarixia radiata, an ectoparasitoid of the Asian citrus psyllid, using internal transcribed spacer and cytochrome oxidase subunit I DNA sequences.
The genetic diversity of Tamarixia radiata Waterston (Hymenoptera: Eulophidae) laboratory colonies derived from collections in China, northern Vietnam, Pakistan, and a mixed colony from Taiwan and southern Vietnam was evaluated using the internal transcribed spacer (ITS) region 1, ITS-2, and the 5' end of the cytochrome oxidase subunit I gene. The strains share the same ITS sequence, consistent with the morphological hypothesis that the collections represent a single species. The COI marker was variable and could distinguish the northern Vietnam and Pakistan colonies from each other and from the other colonies. Comparison of COI sequences from field-collected populations of Puerto Rico, Guadeloupe, and Texas indicates that Florida is not a likely source of the introduction into Puerto Rico but is a likely source of the introduction into Texas.
Barr
N B
NB
Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, Edinburg, TX 78541, USA. norman.b.barr@aphis.usda.gov
Hall
D G
DG
Weathersbee
A A
AA
Nguyen
R
R
Stansly
P
P
Qureshi
J A
JA
Flores
D
D
D000818:Animals; D001208:Asia; D002957:Citrus / Q000469:parasitology; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D049810:Genetic Speciation; D014644:Genetic Variation; D006430:Hemiptera / Q000469:parasitology*; D006790:Host-Parasite Interactions; D032101:Murraya / Q000469:parasitology; D010572:Pest Control, Biological; D014863:Wasps / Q000235:genetics*
D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV