title: [Constituents from Alternanthera philoxeroides and their antitumor activity].
abstract: OBJECTIVE
  To investigate antitumor constituents from n-butyl alcohol extract of Alternanthera philoxeroides.
  
  
  METHODS
  The constituents were isolated with silica gel, gel permeation chromatography, and purified by HPLC. Their structures were elucidated by spectroscopy and acid hydrolysis. The antitumor effects of extracts and isolated compounds were tested by MTH method in vitro.
  
  
  RESULTS
  Seven compounds were isolated and elucidated as followings: oleanolic acid 3-O-beta-D-glucuronopyranoside (1), oleanolic acid 28-O-beta-D-glucopyranoside (2), oleanolic acid 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (3), chikusetsusaponin IV a methyl ester (4), hederagenin 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (5), 4,5-dihydroblumenol (6), 6S,7E,9R-6,9-di-hydroxymegastigma-4,7-dien-3-one-9-O-beta-D-glucopyranoside (7). Compound 1 showed significant inhibitory effect against Hela and L929 with inhibitive ratios 91.3% and 92.9% at 30 mg x L(-1), respectively.
  
  
  CONCLUSIONS
  Compounds 4, 5 and 7 were isolated from this genus for the first time. Compound 1 showed significant inhibitory effect against Hela and L929 at 30 mg x L(-1).
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Fang
authors.forename: Jiachun
authors.initials: J
authors.lastname: Chen
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal