title: Nuclear proteome analysis of monkey embryonic stem cells during differentiation.
abstract: The nuclear proteome enables, manages, and regulates the genome by the collective actions and interactions of proteins found in the nucleus. We applied a proteomic approach to analyze a nuclear proteome during embryonic stem cell (ESC) proliferation, and 3 and 9 days after initiation of differentiation. The nuclei were isolated from cells and their proteins were separated using 2-DE. Out of about 560 protein spots reproducible detected on any give gel, 49 differentially expressed proteins were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF/TOF) mass spectrometry. Of them, several nuclear located proteins involved in chromatin remodeling, transcription regulation, apoptosis, cell proliferation, and differentiation were identified including CTBP1, MM-1, RUVBL1, HCC-1, SGTA, SUMO2, and Galectin-1. Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53.
authors.affiliation: Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box: 19395-4644, Karaj, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Nasrabadi
authors.forename: Mehran Rezaei
authors.initials: MR
authors.lastname: Larijani
authors.forename: Ali
authors.initials: A
authors.lastname: Fathi
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
authors.forename: Ahmad V
authors.initials: AV
authors.lastname: Dizaj
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D008252:Macaca fascicularis; D009687:Nuclear Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D009687:Nuclear Proteins