title: Expression of cyclooxygenase-2 in eutopic endometrium and ovarian endometriotic tissue in women with severe endometriosis.
abstract: OBJECTIVE
  The purpose of this study is to evaluate the level and pattern of cyclooxygenase-2 (Cox-2) expression in endometriosis and to investigate the correlation between the expression of Cox-2 and several clinicosurgical parameters.
  
  
  METHODS
  Twenty-six patients with endometriosis and 21 patients with other benign gynecological conditions were enrolled. The eutopic endometrium was sampled by pipelle, and fragments of ovarian endometrioma and non-endometriotic ovarian cysts were sampled during surgery. Total RNA isolation and semiquantitative reverse transcriptase polymerase chain reaction was performed.
  
  
  RESULTS
  The expression of Cox-2 mRNA (mean +/- SEM) in eutopic endometrium and ovarian endometriotic tissue significantly increased in patients with endometriosis compared with the controls. The expression of Cox-2 increased significantly in the proliferative phase in eutopic endometrium and in the secretory phase in ovarian endometriotic tissue of patients with endometriosis compared with the controls. Cox-2 mRNA expression in the endometrium and ovarian lesions significantly correlated with serum CA-125 and the diameter of the endometrioma.
  
  
  CONCLUSIONS
  Cox-2 expression increased in the eutopic endometrium and ovarian endometriotic tissue of the patients with endometriosis. These findings indicate that Cox-2 may be involved in the pathogenesis and progression of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Gangnam-gu, Seoul, Korea.
authors.forename: SiHyun
authors.initials: S
authors.lastname: Cho
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Park
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Choi
authors.forename: Seok Kyo
authors.initials: SK
authors.lastname: Seo
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Kim
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Park
authors.forename: Dong Jae
authors.initials: DJ
authors.lastname: Cho
authors.forename: Byung Seok
authors.initials: BS
authors.lastname: Lee
mesh_terms: D000328:Adult; D018394:CA-125 Antigen / Q000097:blood; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D004715:Endometriosis / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D010049:Ovarian Diseases / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D018394:CA-125 Antigen; D012333:RNA, Messenger; D051546:Cyclooxygenase 2