LXRbeta deficient mice have reduced hepatic insulin clearance during hyperinsulinemic euglucemic clamp.
The present study addresses the insulin sensitivity in mice deficient in LXRbeta (LXRbeta(-/-)) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRbeta(-/-) mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRbeta(-/-) mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRbeta(-/-) mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRbeta(-/-) mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26weeks.
Holm
Sverre
S
Department of Nutrition, University of Oslo, Blindern, N-0316 Oslo, Norway. sverre.holm@rr-research.no
Voshol
Peter J
PJ
Gustafsson
Jan-Ake
JA
Nebb
Hilde I
HI
D000818:Animals; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000009:adverse effects; D004032:Diet / Q000009:adverse effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D005234:Fatty Liver / Q000235:genetics; D015309:Glucose Clamp Technique; D006505:Hepatitis / Q000235:genetics*; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007333:Insulin Resistance / Q000235:genetics*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism
D002791:Cholesterol, Dietary; D004041:Dietary Fats; D007328:Insulin; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D014280:Triglycerides