title: Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy.
abstract: In this study, stress in relation to slaughter was investigated in a model system by the use of (13)C, (1)H, and (31)P nuclear magnetic resonance (NMR) spectroscopy for elucidating changes in the metabolites in C2C12 myotubes exposed to H(2)O(2)-induced stress. Oxidative stress resulted in lower levels of several metabolites, mainly amino acids; however, higher levels of alanine were apparent in the (13)C spectra after incubation with [(13)C(1)]glucose. In the (13)C spectra [(13)C(3)]lactate tended to increase after exposure to increasing concentrations of H(2)O(2); conversely, a tendency to lower levels of the unlabeled ((12)C) lactate were identified in the (1)H spectra after stress exposure. These data indicate an increase in de novo synthesis of alanine, concomitant with a release of lactate from the myotubes to the medium at oxidative stress conditions. The changes in the metabolite levels could possibly be useful as markers for meat quality traits.
authors.affiliation: Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, DK-8830 Tjele, Denmark.
authors.forename: Ida K
authors.initials: IK
authors.lastname: Straadt
authors.forename: Jette F
authors.initials: JF
authors.lastname: Young
authors.forename: Bent O
authors.initials: BO
authors.lastname: Petersen
authors.forename: Jens Ø
authors.initials: JØ
authors.lastname: Duus
authors.forename: Niels
authors.initials: N
authors.lastname: Gregersen
authors.forename: Peter
authors.initials: P
authors.lastname: Bross
authors.forename: Niels
authors.initials: N
authors.lastname: Oksbjerg
authors.forename: Peter K
authors.initials: PK
authors.lastname: Theil
authors.forename: Hanne C
authors.initials: HC
authors.lastname: Bertram
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress
chemical_list: D000596:Amino Acids; D006861:Hydrogen Peroxide; D005947:Glucose