Quantitation of fentanyl in blood and urine using gas chromatography-mass spectrometry (GC-MS).
Fentanyl is a potent, short-acting synthetic opioid analgesic. Fentanyl is measured in blood and urine following mixed-mode solid phase extraction. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. The final extracts are reconstituted in methanol and analyzed using selected ion monitoring gas chromatography-mass spectrometry.
Goldberger
Bruce A
BA
Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Chronister
Chris W
CW
Merves
Michele L
ML
D005283:Fentanyl / Q000097:blood* / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
D005283:Fentanyl