Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea.
A moderately halophilic Gram-staining-negative bacterium, designated strain Y26(T), was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26(T) was observed at 15-35&emsp14;°C (optimum 25-30°C), pH 6.0-8.0 (optimum pH6.5-7.5) and with 1.5-6.0 % (w/v) NaCl (optimum 2.0-3.0 %). The predominant fatty acids were C(18 : 1)ω7c (66.2 %), C(16 : 0) (12.4 %) and C(10 : 0) 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0&emsp14;mol%. Strain Y26(T) contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26(T) formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8-94.6 % sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26(T) represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26(T) (=KACC 13705(T) =DSM 22009(T)).
Kim
Jeong Myeong
JM
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Jung
Ji Young
JY
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Chae
Ho Byoung
HB
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Park
Woojun
W
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-701, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2