Broad spectrum drug screening using liquid chromatography-hybrid triple quadrupole linear ion trap mass spectrometry.
Centrifuged urine, internal standard (promazine), and ammonium formate buffer are mixed in an autosampler vial to achieve a 10-fold dilution of the specimen. Without additional pretreatment, 10 microL of the sample is injected onto a C18 reverse phase column for gradient analysis with ammonium formate/acetonitrile mobile phases. Drugs in the column eluent become charged in the ion source using positive electrospray atmospheric pressure ionization. Pseudomolecular drug ions are analyzed by a hybrid triple quadrupole linear ion trap mass spectrometer operated with a 264-drug selected ion monitoring (SRM) acquisition method that includes an information-dependant acquisition (IDA) algorithm.
Stone
Judy
J
Clinical Laboratories, San Francisco General Hospital, San Francisco, CA 94804, USA.
D000097:Acetonitriles / Q000737:chemistry; D000465:Algorithms; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006801:Humans; D004364:Pharmaceutical Preparations / Q000652:urine*; D011395:Promazine / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000097:Acetonitriles; D005561:Formates; D004364:Pharmaceutical Preparations; C030544:formic acid; D011395:Promazine; C032159:acetonitrile