title: Arabidopsis thaliana cells: a model to evaluate the virulence of Pectobacterium carotovorum.
abstract: Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall-degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.
authors.affiliation: LEM-EA3514-Universite Paris Diderot-Paris 7, 2 place Jussieu, 75251 Paris cedex 05, France.
authors.forename: Meriam
authors.initials: M
authors.lastname: Terta
authors.forename: Mohamed
authors.initials: M
authors.lastname: Kettani-Halabi
authors.forename: Khadija
authors.initials: K
authors.lastname: Ibenyassine
authors.forename: Daniel
authors.initials: D
authors.lastname: Tran
authors.forename: Patrice
authors.initials: P
authors.lastname: Meimoun
authors.forename: Raja Ait
authors.initials: RA
authors.lastname: M'hand
authors.forename: Hayat
authors.initials: H
authors.lastname: El-Maarouf-Bouteau
authors.forename: Florence
authors.initials: F
authors.lastname: Val
authors.forename: M Mustapha
authors.initials: MM
authors.lastname: Ennaji
authors.forename: François
authors.initials: F
authors.lastname: Bouteau
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000382:microbiology*; D016923:Cell Death; D008954:Models, Biological; D016973:Pectobacterium carotovorum / Q000201:enzymology / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D011133:Polysaccharide-Lyases; C023911:pectate lyase