Radioimmunotherapy of human colon cancer xenografts with 131I-labeled anti-CEA monoclonal antibody.
Radioimmunotherapy (RIT) is a promising approach for the treatment of a wide variety of malignancies. The aim of this study was to investigate the therapeutic efficacy of (131)I-labeled anticarcinoembryonic antigen (CEA) monoclonal antibody CL58 in a human colon cancer mouse model. In vitro and in vivo characteristics of (125)I-CL58 were evaluated in LS180 human colon cancer cells and the nude mouse model. (131)I-CL58 was prepared and its in vivo therapeutic efficacy was tested. (125)I-CL58 showed high affinity to LS180 cells, as well as high tumor uptake and long tumor retention in LS180 tumor xenografts. (131)I-CL58 exhibited dose-dependent inhibition of LS180 tumor growth. With the excellent in vitro and in vivo characteristics, and the effective therapy for colon cancer in animal model, (131)I-CL58 is a promising agent for RIT of CEA-positive tumors including colon cancer.
Liu
Zhaofei
Z
Medical Isotopes Research Center, Peking University, Beijing 100191, China.
Jin
Cunjing
C
Yu
Zilin
Z
Zhang
Jing
J
Liu
Yan
Y
Zhao
Huiyun
H
Jia
Bing
B
Wang
Fan
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology* / Q000532:radiotherapy*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry; D051379:Mice; D016499:Radioimmunotherapy / Q000379:methods*; D011877:Radionuclide Imaging; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007457:Iodine Radioisotopes