title: Studies on biomarkers for oxidative stress in patients with chronic myeloid leukemia.
abstract: BACKGROUND
  Chronic myeloid leukemia (CML) is a myeloproliferative disorder with a unique genetic rearrangement, the Philadelphia chromosome. High reactive oxygen species (ROS) levels favor oxidative stress, which could play a vital role in normal processes and various pathophysiologies including neoplasm. Biomarkers of oxidative stress are measured as products of oxidized proteins and lipids. Plasma levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and total lipid hydroperoxide (LOOH) were used as biomarkers of oxidative stress in the past. The aim of this study was to evaluate the products of protein oxidation and lipid peroxidation in plasma as biomarkers of oxidative stress in CML patients.
  
  
  METHODS
  The study included 40 CML patients and 20 age- and sex-matched healthy volunteers. Of 40 CML patients, 28 were in chronic phase (CML-CP) and 12 in accelerated phase (CML-AP). Plasma levels of PC, TBARS and LOOH as biomarkers of oxidative stress were evaluated by spectrophotometric methods.
  
  
  RESULTS
  There were significant differences (P < .05) in plasma levels of PC, TBARS and LOOH in CML, CML-CP and CML-AP patients as compared to controls.
  
  
  CONCLUSIONS
  PC, TBARS and LOOH might reflect oxidative stress in CML patients and might be used as biomarkers in such patients.
authors.affiliation: Departments of Biochemistry, Chhatrapati Shahvji Maharaj Medical University, Lucknow, Utter Pradesh, India. singhrk23a@hotmail.com
authors.forename: Raj K
authors.initials: RK
authors.lastname: Singh
authors.forename: Anil K
authors.initials: AK
authors.lastname: Tripathi
authors.forename: Payal
authors.initials: P
authors.lastname: Tripathi
authors.forename: Sushma
authors.initials: S
authors.lastname: Singh
authors.forename: Ranjana
authors.initials: R
authors.lastname: Singh
authors.forename: Rizwan
authors.initials: R
authors.lastname: Ahmad
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000473:pathology*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances