[Human enterovirus 71 was isolated from specimens of hand foot mouth disease in Beijing in 2007].
OBJECTIVE
Analyzing and identifying the type of enterovirus of human Hand Foot Mouth Disease (HFMD) outbreak in Daxing district in Beijing at the end of May in 2007.


METHODS
The liquid of Herpes, throat swab and stool specimen were collected. Using reverse transcription-polymerase chain reaction (RT-PCR) method to amplify the enterovirus specific nucleotide acid fragment from specimens and virus positive cultures, the sensitivity of two methods was compared. Then identifying and typing of the enterovirus of HFMD through analyzing the results of nucleotide sequencing of the virus positive cultures.


RESULTS
In 10 specimens from 5 HFMD children patients, enterovirus specific nucleotide acid fragment was detected in 8 patients, the RT-PCR positive ratio (80%) was higher than enterovirus isolation positive ratio (30%). In 5 enteroviruses isolated from 5 patients and 9 close contacts, 4 isolated from 2 patients and 1 close contact were Enterovirus 71.1 isolated from close contact was Coxsackievirus A16.


CONCLUSIONS
Enterovirus 71 was the pathogen of HFMD outbreak in Daxing district at the end of May in 2007.
Li
Ren-Qing
RQ
Beijing City Center for Disease Control and Prevention, Beijing 100013, China.
Chen
Li-Juan
LJ
Zhang
He-Run
HR
D002460:Cell Line; D002681:China; D029821:Enterovirus A, Human / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D006232:Hand, Foot and Mouth Disease / Q000635:transmission / Q000821:virology*; D006801:Humans; D010614:Pharynx / Q000821:virology*