[Cloning and characterization of open reading frame encoding beta-amyrin synthase in Glycyrrhiza uralensis].
OBJECTIVE
To clone and sequence the open reading frame of beta-amyrin synthase (bAS) from Glycyrrhiza uralensis.


METHODS
The primers were designed according to the cDNA sequence of beta-amyrin synthase from G. glabra reported by Hiroaki HAYASHI, and the open reading frame of beta-amyrin synthase was cloned by RT-PCR strategy with the template of total RNA extracted from roots of G. uralensis.


RESULTS
The GubAS (GenBank Accession number: FJ627179) was 2 289 bp in length encoding one pelypeptide of 762 amino acid. Deduced amino acid sequence had 99%, 92%, 90%, 90% and 89% homology to the amino acid sequence of G. glabra, Lotus japonicus, Pisum sativum, Medicago truncatula, Glycine max, respectively.


CONCLUSIONS
The open reading frame of bAS from G. uralensis is cloned and reported for the first time. The conclusion will provide a foundation for exploring the mechanism of triterpenes biosynthesis.
Shen
Zhanyun
Z
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Liu
Chunsheng
C
Wang
Xueyong
X
D003001:Cloning, Molecular; D029882:Glycyrrhiza uralensis / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000145:classification / Q000235:genetics
D010940:Plant Proteins; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase