title: Platelet-rich plasma, rhOP-1 (rhBMP-7) and frozen rib allograft for the reconstruction of bony mandibular defects in sheep. A pilot experimental study.
abstract: A 6 cm bony defect in the mandible of 15 sheep, 8 years old, was reconstructed using variously allograft of frozen rib, rhOP-1 (rh BMP-7), platelet-rich plasma (PRP), and a combination of frozen rib allograft and rhOP-1. The histological, histomorphometric, immunohistochemical and radiographic features of reconstruction were analysed. The animals were euthanised at 2 months postoperatively. In the control and PRP groups, no bone formation was detected. The sheep receiving rhOP-1 showed some and those receiving both rhOP-1 and allograft showed most new bone formation; in both groups this was through endochondral and also fibrous ossification. The combination of bone allograft with growth factors demonstrated osteoconductive as well as osteoinductive properties, and is appealing in the management of problem fractures.
authors.affiliation: Research Unit FREMAP Hospital, Ctra Pozuelo 61, 28220 Majadahonda, Madrid, Spain. fforiol@gmail.com
authors.forename: Francisco
authors.initials: F
authors.lastname: Forriol
authors.forename: Umile Giuseppe
authors.initials: UG
authors.lastname: Longo
authors.forename: Carlos
authors.initials: C
authors.lastname: Concejo
authors.forename: Purificacion
authors.initials: P
authors.lastname: Ripalda
authors.forename: Nicola
authors.initials: N
authors.lastname: Maffulli
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Denaro
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D055419:Bone Morphogenetic Protein 7 / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods; D004337:Drug Carriers; D020007:Implants, Experimental; D008334:Mandible / Q000000981:diagnostic imaging / Q000601:surgery*; D023421:Models, Animal; D010027:Osteotomy; D010865:Pilot Projects; D053657:Platelet-Rich Plasma; D011859:Radiography; D011897:Random Allocation; D012272:Ribs / Q000637:transplantation*; D012756:Sheep; D013048:Specimen Handling; D014184:Transplantation, Homologous
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D004337:Drug Carriers