Hydrophilic biopolymer grafted on poly(dimethylsiloxane) surface for microchip electrophoresis.
A novel covalent strategy was developed to modify the poly(dimethylsiloxane) (PDMS) surface. Briefly, dextran was selectively oxidized to aldehyde groups with sodium periodate and subsequently grafted onto amine-functionalized PDMS surface via Schiff base reaction. As expected, the coated PDMS surface efficiently prevented the biomolecules from adsorption. Electro-osmotic flow (EOF) was successfully suppressed compared with that on the native PDMS microchip. Moreover, the stability of EOF was greatly enhanced and the hydrophilicity of PDMS surface was also improved. To apply thus-coated microchip, the separation of peptides, protein and neurotransmitters was investigated in detail. For comparison, these analytes were also measured on the native PDMS microchips. The results demonstrated that these analytes were efficiently separated and detected on the coated PDMS microchips. Furthermore, the relative standard deviations of their migration times for run-to-run, day-to-day, and chip-to-chip reproducibilities were in the range of 0.6-2.7%. In addition, the coated PDMS microchips showed good stability within 1 month.
Feng
Jiu-Ju
JJ
School of Chemistry and Environmental Science, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Jianshe Road, Xinxiang, Henan 453007, China.
Wang
Ai-Jun
AJ
Fan
Jing
J
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
D001704:Biopolymers / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010504:Periodic Acid / Q000737:chemistry; D010544:Peroxidases / Q000032:analysis; D013499:Surface Properties
D001704:Biopolymers; D004129:Dimethylpolysiloxanes; D018377:Neurotransmitter Agents; D010455:Peptides; D010504:Periodic Acid; C009288:metaperiodate; D010544:Peroxidases