[Establishment of transgenic receptor system and aFGF transformation in astragalus].
OBJECTIVE
To establish a high-frequency regeneration system of Astragalus and an aFGF transformation system.


METHODS
Cotyledon node of the Astragalus explants was used for organogenesis to establish a high-frequency regeneration system. GV3101 was used to transform cotyledon node, and aFGF gene was introduced into Astragalus, renewable strain was detected by PCR.


RESULTS
All cotyledon node was explants, adventitious buds were induced in the medium of MS +2.0 mg x L(-1) BA +0.5 mg x L(-1) IBA, the root was taken in the medium of 1/2MS +5.0 mg x L(-1) NAA to give a high frequency regeneration system. All cotyledon node was precultured in medium for 3 days and infected with Agrobacterium (A600 0.3) for 10 min and then cocultured for 2 days. The aFGF gene was confirmed to transform into genome of Astragalus.


CONCLUSIONS
A high-frequency regeneration system of Astragalus and an aFGF transformation system were established.
Li
Dachao
D
College of Life Science, Jilin Agricultural University, Changchun 130118, China.
Wang
Yi
Y
Jiang
Shicui
S
Han
Xiuwen
X
Zhang
Meiping
M
D027883:Astragalus Plant / Q000235:genetics* / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D016220:Fibroblast Growth Factor 1