Optimization of mono and diacylglycerols production from enzymatic glycerolysis in solvent-free systems.
This work reports solvent-free enzymatic glycerolysis of olive oil with an immobilized lipase (Novozym 435) using Tween 40, Tween 65, Tween 80, Tween 85, Triton X-100, and soy lecithin as surfactants. The first step was the screening of two potential surfactants for Monoacylglycerol (MAG) and Diacylglycerol (DAG) production with a pre-established operating condition and 2 h of reaction time. Afterwards, a sequential experimental design strategy was carried out in order to optimize MAG and DAG production using Tween 65 and Triton X-100 as surfactants. The operating conditions that optimized MAG and DAG yields were 70 degrees C, stirring rate of 600 rpm, glycerol:olive oil molar ratio of 6:1, 16 wt% of surfactant Tween 65 and 9.0 wt% of Novozym 435, leading to a content of 26 and 17 wt% of MAG and DAG, respectively.
Valério
Alexsandra
A
Department of Food Engineering, URI-Campus de Erechim, Erechim, RS 99700-000, Brazil.
Rovani
Suzimara
S
Treichel
Helen
H
de Oliveira
Débora
D
Oliveira
J Vladimir
JV
D004075:Diglycerides / Q000138:chemical synthesis* / Q000302:isolation & purification*; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; D013501:Surface-Active Agents; C093667:Novozyme 435; D008049:Lipase