Liposomal siRNA delivery.
With the recent discovery of small interfering RNA (siRNA), to silence the expression of genes in vitro and in vivo, there has been a need to deliver these molecules to the cell nucleus. Forming a lipid/nucleic acid complex has become a solution and is explored here. Certain methods and ideas are used, such as: the positive/negative electrostatic interaction with a cationic lipid and an anionic RNA molecule, the size of the lipid vesicle aiding the uptake target tissues, targeted lipoplexes which can increase efficiency, and the protection of the siRNA molecule from the natural defenses of the immune system. Many lipid formulations exist and can be experimented with to achieve varying results depending on the application.
Hughes
Jeffrey
J
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA.
Yadava
Preeti
P
Mesaros
Ryan
R
D000818:Animals; D002118:Calcium / Q000737:chemistry; D002478:Cells, Cultured; D000431:Ethanol / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D014162:Transfection
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; D000431:Ethanol; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; D002118:Calcium