Identification of pyrazosulfuron-ethyl binding affinity and binding site subdomain IIA in human serum albumin by spectroscopic methods.
Pyrazosulfuron-ethyl (PY) is a sulfonylurea herbicide developed by DuPont which has been widely used for weed control in cereals. The determination of PY binding affinity and binding site in human serum albumin (HSA) by spectroscopic methods is the subject of this work. From the fluorescence emission, circular dichroism and three-dimensional fluorescence results, the interaction of PY with HSA caused secondary structure changes in the protein. Fluorescence data demonstrated that the quenching of HSA fluorescence by PY was the result of the formation of HSA-PY complex at 1:1 molar ratio, a static mechanism was confirmed to lead to the fluorescence quenching. Hydrophobic probe 8-anilino-1-naphthalenesulfonic acid (ANS) displacement results show that hydrophobic patches are the major sites for PY binding on HSA. The thermodynamic parameters DeltaH degrees and DeltaS degrees were calculated to be -36.32 kJ mol(-1) and -35.91 J mol(-1)K(-1), which illustrated van der Waals forces and hydrogen bonds interactions were the dominant intermolecular force in stabilizing the complex. Also, site marker competitive experiments showed that the binding of PY to HSA took place primarily in subdomain IIA (Sudlow's site I). What presented in this paper binding research enriches our knowledge of the interaction between sulfonylurea herbicides and the physiologically important protein HSA.
Ding
Fei
F
Department of Chemistry, China Agricultural University, Haidian District, Beijing, China.
Liu
Wei
W
Zhang
Xi
X
Wu
Li-Jun
LJ
Zhang
Li
L
Sun
Ying
Y
D001665:Binding Sites; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D011720:Pyrazoles / Q000737:chemistry / Q000378:metabolism; D011743:Pyrimidines / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*; D013816:Thermodynamics
D006540:Herbicides; D011720:Pyrazoles; D011743:Pyrimidines; D012709:Serum Albumin; C433343:ethyl 5-(4,6-dimethoxypyrimidin-2-ylcarbamoylsulfamoyl)-1-methylpyrazole-4-carboxylate