title: Investigation of human papillomavirus DNA in colorectal carcinomas and adenomas.
abstract: Human papillomavirus (HPV) has been considered to be an etiological agent for anogenital cancers, such as cervical cancer and possibly a subset of cancers of the aerodigestive tract. The aim of the study was to evaluate the presence of human papillomavirus DNA in colorectal carcinomas and adenomas. Formalin-fixed and paraffin-embedded archival tissue samples were used for DNA extraction. One hundred and six colorectal carcinomas and 62 adenomas were screened by nested polymerase chain reaction (PCR) for HPV DNA with a control group of 49 cervical tissues with invasive cervical carcinoma and cervical intraepithelial neoplasia (CIN). In the study group, we did not find HPV DNA positivity in any of all the colorectal carcinomas and adenomas. In the control group with cervical lesions, 34 out of 49 (69.4%) samples were positive for the HPV DNA. These results indicated that there was no correlation between HPV infection and colorectal carcinomas and adenomas.
authors.affiliation: Department of Pathology, Dr Lutfi Kirdar Kartal Training and Research Hospital, 34865 Cevizli/Istanbul, Turkey. dilekyavuzer@yahoo.com
authors.forename: Dilek
authors.initials: D
authors.lastname: Yavuzer
authors.forename: Nimet
authors.initials: N
authors.lastname: Karadayi
authors.forename: Taflan
authors.initials: T
authors.lastname: Salepci
authors.forename: Huseyin
authors.initials: H
authors.lastname: Baloglu
authors.forename: Resat
authors.initials: R
authors.lastname: Dabak
authors.forename: Oya Uygur
authors.initials: OU
authors.lastname: Bayramicli
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000821:virology*; D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000821:virology; D000236:Adenoma / Q000235:genetics / Q000821:virology*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000821:virology
chemical_list: D004279:DNA, Viral