Rapid determination of nanotoxicity using luminous bacteria.
A new luminescence-based toxicity test using luminous bacteria as a reporting agent has been developed to determine EC(50) of different nanomaterials, such as gold nanoparticles and carbon nanotubes on living organisms. The whole assay takes only about 15 min and is as sensitive as other standard methods. Due to its technical simplicity, rapidity and sensitivity, this luminescent bacteria test has the potential to be developed as a general test of toxicity for a wide variety of nanomaterials.
Zheng
Huzhi
H
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, P. R. China. zhenghz@swu.edu.cn
Liu
Li
L
Lu
Yihui
Y
Long
Yijuan
Y
Wang
Lingling
L
Ho
Kam-Piu
KP
Wong
Kwok-Yin
KY
D001419:Bacteria / Q000187:drug effects*; D002244:Carbon / Q000633:toxicity; D005434:Flow Cytometry; D006046:Gold / Q000633:toxicity; D007928:Lethal Dose 50; D049449:Luminescence; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000633:toxicity*; D043942:Nanotubes / Q000633:toxicity; D012710:Serum Albumin, Bovine / Q000737:chemistry
D012710:Serum Albumin, Bovine; D002244:Carbon; D006046:Gold