Imaging mass spectrometry using a delay-line detector.
Microscope mode mass spectrometric imaging is crucially dependent on the availability of a high- resolution, position-resolved time-of-flight detector. Here, a new detection method for microscope mode imaging mass spectrometry is presented. A delay-line detector has been used for the first time as a position-sensitive detector in imaging mass spectrometry. The method is implemented on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF), as well as a secondary ion mass spectrometry time-of-flight (SIMS-ToF) instrument. Trypsinogen and bovine serum albumin samples have been used with a metal mask to determine the spatial resolution of the new detector using the MALDI-ToF instrument. The new detector set-up was successfully employed to generate mass resolved SIMS images from biological structures on the surface of thin tissue sections. The biological samples studied were taken from tumor grown from xenografted breast cancer cell lines and chicken embryonal sections.
Froesch
Martin
M
FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam, The Netherlands.
Luxembourg
Stefan L
SL
Verheijde
Duncan
D
Heeren
Ron M A
RM
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002417:Cattle; D045744:Cell Line, Tumor; D002642:Chick Embryo; D004867:Equipment Design; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods
D012710:Serum Albumin, Bovine