title: Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin.
abstract: With an increase in the importance of umbilical cord blood (CB) as an alternative source of haematopoietic progenitors for allogenic transplantation, donor lymphocyte infusion (DLI) with donor CB-derived activated CD4(+) T cells in the unrelated CB transplantation setting is expected to be of increased usefulness as a direct approach for improving post-transplant immune function. To clarify the characteristics of activated CD4(+) T cells derived from CB, we investigated their mRNA expression profiles and compared them with those of peripheral blood (PB)-derived activated CD4(+) T cells. Based on the results of a DNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), a relatively high level of forkhead box protein 3 (Foxp3) gene expression and a relatively low level of interleukin (IL)-17 gene expression were revealed to be significant features of the gene expression profile of CB-derived activated CD4(+) T cells. Flow cytometric analysis further revealed protein expression of Foxp3 in a portion of CB-derived activated CD4(+) T cells. The low level of retinoic acid receptor-related orphan receptor gamma isoform t (RORgamma t) gene expression in CB-derived activated CD4(+) T cells was speculated to be responsible for the low level of IL-17 gene expression. Our data indicate a difference in gene expression between CD4(+) T cells from CB and those from PB. The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4(+) T cells may be a more appropriate source for DLI.
authors.affiliation: Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
authors.forename: Nakaba
authors.initials: N
authors.lastname: Ochiai
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Imadome
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Keiko
authors.initials: K
authors.lastname: Onda
authors.forename: Misako
authors.initials: M
authors.lastname: Yajima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Yohko U
authors.initials: YU
authors.lastname: Katagiri
authors.forename: Hajime
authors.initials: H
authors.lastname: Okita
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morio
authors.forename: Norio
authors.initials: N
authors.lastname: Shimizu
authors.forename: Junichiro
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Fujiwara
mesh_terms: D001773:Blood Cells / Q000166:cytology; D001804:Blood Transfusion, Autologous; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D017710:Lymphocyte Transfusion; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis*; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D000097768:Retinoic Acid Receptor gamma
chemical_list: D051858:Forkhead Transcription Factors; D020381:Interleukin-17; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000097768:Retinoic Acid Receptor gamma; C418974:FOXP3 protein, human