title: Dissociation of nystatin and amphotericin analogues: characterisation of minor anti-fungal macrolides.
abstract: Tandem mass spectrometry combined with Fourier transform ion cyclotron resonance (FT-ICR) has been the basis for rationalizing the fragmentation mechanisms of anti-fungal macrolides nystatin A(1), amphotericin B and pimaricin. The positive ion mass spectra were not informative, however, the dissociation of deprotonated molecules led to structurally significant ring-opened fragments. Using this approach of tandem FT-ICR mass spectrometry and electrospray ionisation coupled with high-performance liquid -chromatography (HPLC), 11 macrolide natural analogues or degradation products were characterised in the nystatin mixture.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Ulrych
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Derrick
authors.forename: Frantisek
authors.initials: F
authors.lastname: Adamek
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
authors.forename: Vladimir
authors.initials: V
authors.lastname: Havlicek
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D018942:Macrolides / Q000737:chemistry*; D015394:Molecular Structure; D009761:Nystatin / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000935:Antifungal Agents; D018942:Macrolides; D009761:Nystatin; D000666:Amphotericin B