[Exploration of developmental toxicity mechanism of pentachlorophenol using cDNA microarray].
0hpf zebrafish embryos were exposed to 50 microg/L pentachlorophenol(PCP) for 8h in vitro. Total RNA sample was extracted then and hybridized with Affymetrix Zebrafish Genome Array representing approximately 14 900 transcripts. A total of 1 149 transcripts was significantly up-regulated while 501 transcripts were down-regulated. Bioinformatic tools were used for further analysis. The result indicated that genes with significant expression changes were related to molecular functions including antioxidant activity, signal transducer activity, translation regulator activity, transcription regulator activity, et al. Genes regulated by BMP signals, FGF signals, and Nodal signals including smad2, smad5, bmp4, bmp7, flh, n-ras may involve in the developmental toxicity of PCP, with Signal log ratios of 4.6, 2.1, 1.6, 1.0, 1, 1.3, 1.0, respectively. This investigation may provide new biomarkers to further study of the developmental toxicity of PCP.
Wu
Zhao-Yi
ZY
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China. wuzhaoyi_83@yahoo.com.cn
Hu
Ping
P
Zhao
Qing-Shun
QS
Yin
Da-Qiang
DQ
D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010416:Pentachlorophenol / Q000633:toxicity*; D015027:Zebrafish
D004785:Environmental Pollutants; D010416:Pentachlorophenol