[Determination of pseudoprodioscin in Dioscorea panthaica by HPLC].
OBJECTIVE
To develop an HPLC method for the determination of pseudoprodioscin in Dioscorea panthaica and provide scientific basis for quality control of it and its preparation.


METHODS
The analysis was carried out on a Merck Purospher STAR RP-18e column eluted with acetonitrile and water as mobile phases in gradient mode. The detection wavelength was 203 nm, and the flow rate was 1.0 mL min(-1).


RESULTS
The pseudoprodioscin in D. panthaica extract was well separated. The linear range is between 0.0800-4.00 microg, r =0.9999. The average recovery and RSD are 99.9% and 2.3%, respectively.


CONCLUSIONS
This method for quantitation of pseudoprodioscin in D. panthaica is simple, accurate and reliable and can be used for the quality control of D. panthaica and its preparations.
Jing
Wenguang
W
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Zhang
Qiwei
Q
Liu
An
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D004144:Diosgenin