title: Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.
abstract: The 70-kDa heat shock proteins (Hsp70) are chaperones with central roles in processes that involve polypeptide remodeling events. Hsp70 proteins consist of two major functional domains: an N-terminal nucleotide binding domain (NBD) with ATPase activity, and a C-terminal substrate binding domain (SBD). We present the first crystal structures of four human Hsp70 isoforms, those of the NBDs of HSPA1L, HSPA2, HSPA5 and HSPA6. As previously with Hsp70 family members, all four proteins crystallized in a closed cleft conformation, although a slight cleft opening through rotation of subdomain IIB was observed for the HSPA5-ADP complex. The structures presented here support the view that the NBDs of human Hsp70 function by conserved mechanisms and contribute little to isoform specificity, which instead is brought about by the SBDs and by accessory proteins.
  
  
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authors.affiliation: Structural Genomics Consortium, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wisniewska
authors.forename: Tobias
authors.initials: T
authors.lastname: Karlberg
authors.forename: Lari
authors.initials: L
authors.lastname: Lehtiö
authors.forename: Ida
authors.initials: I
authors.lastname: Johansson
authors.forename: Tetyana
authors.initials: T
authors.lastname: Kotenyova
authors.forename: Martin
authors.initials: M
authors.lastname: Moche
authors.forename: Herwig
authors.initials: H
authors.lastname: Schüler
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry*; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D018076:DNA, Complementary; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; C000717247:HSPA5 protein, human; D020033:Protein Isoforms; D000251:Adenosine Triphosphatases