title: Red-white-blue emission switching molecular beacons: ratiometric multicolour DNA hybridization probes.
abstract: Molecular beacons with two fluorophores derived from ethynyl pyrene and ethynyl nile red exhibit red emission in the hairpin that changes to blue through white upon binding to the target sequence with a dramatic shift of approximately 225 nm.
authors.affiliation: Univeristy of Regensburg, Institute for Organic Chemistry, Universitätsstr. 31, D-93053 Regensburg, Germany.
authors.forename: Reji
authors.initials: R
authors.lastname: Varghese
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D001483:Base Sequence; D003116:Color; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D015335:Molecular Probes; D009838:Oligodeoxyribonucleotides; D010078:Oxazines; D011721:Pyrenes; D004247:DNA; C030984:pyrene; C044808:nile red