title: Simultaneous presence of bovine papillomavirus in blood and in short-term lymphocyte cultures from dairy cattle in Pernambuco, Brazil.
abstract: Bovine papillomaviruses (BPV) are the causal agents of benign and malignant lesions; they can cause dramatic economic losses in cattle. Although 10 virus types have been described, three types are most common in tumors, namely BPV-1, -2 and -4. Previous studies have reported BPV in blood cells and the possibility of blood acting as a latent virus site and/or transmission agent of virus dissemination. We studied a Holstein dairy herd in Pernambuco, Brazil, in which several animals showed severe cutaneous papillomatosis, without previous determination of BPV types. Blood samples and short-term lymphocyte cultures were collected from 54 cows. We compared the BPV types detected in peripheral blood to those identified in the respective lymphocyte cultures: BPV-1 was detected in 74% and BPV-2 in 87% of the whole blood samples. Simultaneous virus presence (BPV-1 and BPV-2) was found in 65% of the blood samples. BPV-1 or BPV-2 were detected in the lymphocyte cultures in 93% of the samples, and both in 89%. The detection of viral DNA in whole blood and in lymphocyte cultures is evidence that this virus is carried by lymphocytes.
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: N
authors.initials: N
authors.lastname: Diniz
authors.forename: T C
authors.initials: TC
authors.lastname: Melo
authors.forename: J F
authors.initials: JF
authors.lastname: Santos
authors.forename: E
authors.initials: E
authors.lastname: Mori
authors.forename: P E
authors.initials: PE
authors.lastname: Brandão
authors.forename: L J
authors.initials: LJ
authors.lastname: Richtzenhain
authors.forename: A C
authors.initials: AC
authors.lastname: Freitas
authors.forename: W
authors.initials: W
authors.lastname: Beçak
authors.forename: R F
authors.initials: RF
authors.lastname: Carvalho
authors.forename: R C
authors.initials: RC
authors.lastname: Stocco
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002478:Cells, Cultured; D004279:DNA, Viral; D003612:Dairying; D008214:Lymphocytes / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014766:Viremia / Q000097:blood*
chemical_list: D004279:DNA, Viral