Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death.
In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.
Sánchez-Torres
L E
LE
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas-IPN, Prol. Carpio y Plan de Ayala s/n, Colonia Santo Tomás, México, D.F., C.P. 11340, México. luviasanchez@hotmail.com
Torres-Martínez
J A
JA
Godínez-Victoria
M
M
Omar
J-M
JM
Velasco-Bejarano
B
B
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D019659:Asteraceae / Q000737:chemistry*; D020169:Caspases / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007536:Isomerism; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism; D008027:Light; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000627:therapeutic use
D000972:Antineoplastic Agents, Phytogenic; D051033:Apoptosis Inducing Factor; D010718:Phosphatidylserines; D010936:Plant Extracts; D012717:Sesquiterpenes; D020169:Caspases