title: A putative transporter is essential for integrating nutrient and hormone signaling with lateral root growth and nodule development in Medicago truncatula.
abstract: Legume root architecture involves not only elaboration of the root system by the formation of lateral roots but also the formation of symbiotic root nodules in association with nitrogen-fixing soil rhizobia. The Medicago truncatula LATD/NIP gene plays an essential role in the development of both primary and lateral roots as well as nodule development. We have cloned the LATD/NIP gene and show that it encodes a member of the NRT1(PTR) transporter family. LATD/NIP is expressed throughout the plant. pLATD/NIP-GFP promoter-reporter fusions in transgenic roots establish the spatial expression of LATD/NIP in primary root, lateral root and nodule meristems and the surrounding cells. Expression of LATD/NIP is regulated by hormones, in particular by abscisic acid which has been previously shown to rescue the primary and lateral root meristem arrest of latd mutants. latd mutants respond normally to ammonium but have defects in responses of the root architecture to nitrate. Taken together, these results suggest that LATD/NIP may encode a nitrate transporter or transporter of another compound.
authors.affiliation: Department of Plant Biology, University of Vermont, Burlington, VT 05405-0086, USA.
authors.forename: Craig R
authors.initials: CR
authors.lastname: Yendrek
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Lee
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Morris
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Catalina I
authors.initials: CI
authors.lastname: Pislariu
authors.forename: Graham
authors.initials: G
authors.lastname: Burkart
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Meckfessel
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehin
authors.forename: Hilary
authors.initials: H
authors.lastname: Kessler
authors.forename: Heath
authors.initials: H
authors.lastname: Wessler
authors.forename: Melanie
authors.initials: M
authors.lastname: Lloyd
authors.forename: Heather
authors.initials: H
authors.lastname: Lutton
authors.forename: Alice
authors.initials: A
authors.lastname: Teillet
authors.forename: D Janine
authors.initials: DJ
authors.lastname: Sherrier
authors.forename: Etienne-Pascal
authors.initials: EP
authors.lastname: Journet
authors.forename: Jeanne M
authors.initials: JM
authors.lastname: Harris
authors.forename: Rebecca
authors.initials: R
authors.lastname: Dickstein
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055170:Plant Root Nodulation; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics
chemical_list: D026901:Membrane Transport Proteins; D009566:Nitrates; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D018749:RNA, Plant; D000040:Abscisic Acid