title: Analysis of immunoreaction with localized surface plasmon resonance biosensor.
abstract: The localized surface plasmon resonance (LSPR)-based optical biosensor was used as a potential tool for label-free detection of immunoreaction. The glass substrate covered with the self-assembled monolayer (SAM) of gold colloids was used widely in the sensors. Here, the glass substrate was modified by chemical hydroxylation first, and then gold colloids were immobilized on the substrate by electrostatic adsorption. The LSPR spectra were obtained on UV-vis absorption spectrometer. The specificity was examined by extensive nonspecific binding tests. The resonance condition on the local dielectric environment enables a simple form of molecular sensing. The binding of analyte to the biosensor surface causes a change in the absorbance which was responsive to the concentration of human IgG. So, the LSPR sensing yields similar results to the SPR technique, yet with much simpler instrument.
authors.affiliation: College of Chemistry, Jilin University, Changchun, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Bi
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D005898:Glass / Q000737:chemistry; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D055672:Static Electricity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013499:Surface Properties
chemical_list: D017979:Gold Colloid; D007074:Immunoglobulin G