title: Green tea catechins and their oxidative protection in the rat eye.
abstract: Catechins, active constituents of green tea, are well-known antioxidative natural products. It was proposed that green tea extract (GTE) consumption could benefit the eye, and the pharmacokinetics of catechins and oxidation status in rat eye were investigated after oral administration. Sprague-Dawley rats were fed GTE and sacrificed at different time intervals. Their eyes were dissected into cornea, lens, retina, choroid-sclera, vitreous humor, and aqueous humor for analysis of catechins and 8-epi-isoprostane by HPLC-ECD and GC-NCI-MS, respectively. Catechins were differentially distributed in eye tissues. Gallocatechin was present at the highest concentration in the retina, 22729.4 +/- 4229.4 pmol/g, and epigallocatechin in aqueous humor at 602.9 +/- 116.7 nM. The corresponding area-under-curves were 207,000 pmol x h/g and 2035.0 +/- 531.7 nM x h, respectively. The time of maximum concentration of the catechins varied from 0.5 to 12.2 h. Significant reductions in 8-epi-isoprostane levels were found in the compartments except the choroid-sclera or plasma, indicating antioxidative activities of catechins in these tissues.
authors.affiliation: Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Kowloon, Hong Kong.
authors.forename: Kai On
authors.initials: KO
authors.lastname: Chu
authors.forename: Kwok Ping
authors.initials: KP
authors.lastname: Chan
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
authors.forename: Ching Yan
authors.initials: CY
authors.lastname: Chu
authors.forename: Wai Ying
authors.initials: WY
authors.lastname: Li
authors.forename: Kwong Wai
authors.initials: KW
authors.lastname: Choy
authors.forename: Michael Scott
authors.initials: MS
authors.lastname: Rogers
authors.forename: Chi Pui
authors.initials: CP
authors.lastname: Pang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002392:Catechin