title: The bovine annexin 9 gene (ANXA9) is significantly associated with milk-fat yield in a Spanish Holstein-Friesian population.
abstract: On the basis of QTL studies for milk-fat yield trait on BTA3, annexin 9 protein (ANXA9), fatty acid transport protein type 3 (SLC27A3) and diacylglycerol O-acyltransferase 1 (DGAT1) were selected as candidate genes. Three different single nucleotide polymorphisms (SNPs) of bovine ANXA9, SLC27A3 and DGAT1 genes have been tested in a selective genotyping design for milk-fat yield. Significant allele frequency differences were found for ANXA9 (p=0.02), in Holstein-Friesian animals with high and low breeding values for milk-fat yield. Regression analysis also showed a significant effect (p=0.0207) between estimated breeding values (EBVs) for fat milk content and ANXA9 polymorphism. So ANXA9 gene falls into a significant quantitative trait loci interval for milk-fat yield that was previously reported on bovine chromosome 3 in other dairy populations. Our results suggest that the ANXA9 gene polymorphism or a linked segregating QTL contributes to variation in milk-fat yield.
authors.affiliation: Unidad de Tecnología en Producción Animal, CITA-Gobierno de Aragón, 50059 Zaragoza, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Royo
authors.forename: L
authors.initials: L
authors.lastname: Ordovas
authors.forename: P
authors.initials: P
authors.lastname: Zaragoza
authors.forename: J
authors.initials: J
authors.lastname: Altarriba
authors.forename: M
authors.initials: M
authors.lastname: Serrano
authors.forename: C
authors.initials: C
authors.lastname: Rodellar
authors.forename: J H
authors.initials: JH
authors.lastname: Calvo
mesh_terms: D000818:Animals; D017302:Annexins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics; D050612:Fatty Acid Transport Proteins / Q000235:genetics; D005260:Female; D005838:Genotype; D008055:Lipids / Q000032:analysis*; D008892:Milk / Q000737:chemistry* / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013030:Spain; D013045:Species Specificity
chemical_list: D017302:Annexins; D017931:DNA Primers; D050612:Fatty Acid Transport Proteins; D008055:Lipids; D051048:Diacylglycerol O-Acyltransferase