title: Chemical rescue of protein tyrosine phosphatase activity.
abstract: We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.
authors.affiliation: Amherst College, Department of Chemistry, Amherst, MA, USA.
authors.forename: Vincent L
authors.initials: VL
authors.lastname: Chen
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Bishop
mesh_terms: D020134:Catalytic Domain; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism*; D015870:Gene Expression; D009154:Mutation; D011485:Protein Binding; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005452:Fluoresceins; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12