Effects on the expression of GABAA receptor subunits by jujuboside A treatment in rat hippocampal neurons.
OBJECTIVE
To determine the effect of jujuboside A (JuA) in modulating the gamma-aminobutyric acid (GABA(A)) receptor subunits gene expression of hippocampal neurons at different terms in vitro.


METHODS
Hippocampal neurons of rat were cultured in vitro, treated with JuA or diazepam (DZP). Then GABA(A) receptor mRNAs were evaluated by semi-quantitative RT-PCR.


RESULTS
JuA at the low dose of 41 microM (about 0.05 g/l) induced significant increase of GABA(A) receptor alpha1, alpha5, beta2 subunit mRNAs in both 24 and 72h treatments. JuA at the high dose of 82 microM (about 0.1g/l) significantly increased GABA(A) receptor alpha1, alpha5 subunit mRNA levels and decreased beta2 subunit mRNA level at 24h treatment, and decreased GABA(A) receptor subunit alpha1, beta2 mRNAs expression at 72h treatment. DZP of 10 microM significantly increased expression of GABA(A) receptor subunit alpha1, alpha5 and decreased expression of beta2 at 24h treatment, and decreased alpha1, alpha5, beta2 subunits gene expression at 72h treatment.


CONCLUSIONS
Differences in alterations in GABA(A) receptor subunit mRNAs expression following JuA and DZP treatments could help to explain the differences in the pharmacological action of the two drugs.
You
Zi-li
ZL
Key Laboratory for NeuroInformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. youzili@uestc.edu.cn
Xia
Qing
Q
Liang
Fan-rong
FR
Tang
Yi-jun
YJ
Xu
Cong-lun
CL
Huang
Jian
J
Zhao
Ling
L
Zhang
Wen-zheng
WZ
He
Jia-jia
JJ
D000818:Animals; D002478:Cells, Cultured; D003975:Diazepam / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
C546242:Gabra1 protein, rat; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D012503:Saponins; C087808:jujuboside A; D005680:gamma-Aminobutyric Acid; D003975:Diazepam