A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.
The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.
Schulz
Stephan
S
Technische Universität München, Chair of Soil Ecology, 85764 Neuherberg, Germany.
Peréz-de-Mora
Alfredo
A
Engel
Marion
M
Munch
Jean Charles
JC
Schloter
Michael
M
D001419:Bacteria / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial; D014644:Genetic Variation; D006899:Mixed Function Oxygenases / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D006899:Mixed Function Oxygenases