Gene expression microarray analysis of heat stress in the soil invertebrate Folsomia candida.
Sudden temperature changes in soil can induce stress in soil-dwelling invertebrates. Hyperthermic conditions have an impact on gene expression as one of the first steps. We use a transcriptomics approach using microarrays to identify expression changes in response to heat in the springtail Folsomia candida. An elevation of temperature (Delta 10 degrees C) altered the expression of 142 genes (116 up-, 26 down-regulated). Many up-regulated genes encoded heat shock proteins, enzymes involved in ATP synthesis, oxidative stress responsive enzymes and anion-transporting ATPases. Down-regulated were glycoside hydrolases, involved in catalysis of disaccharides. The small number of altered transcripts suggest a mild response to heat in this soil invertebrate, but further research is needed to confirm this. This study presents candidate genes for future functional studies concerning thermal stress in soil-dwelling invertebrates.
Nota
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Department of Animal Ecology, VU University Amsterdam, Amsterdam, The Netherlands. benjamin.nota@gmail.com
van Straalen
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Ylstra
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Roelofs
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D
D000818:Animals; D001181:Arthropods / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D018869:Heat-Shock Response / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012987:Soil / Q000469:parasitology*; D013312:Stress, Physiological / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
D012987:Soil