Antiviral activities of diarylheptanoids against influenza virus in vitro.
The anti-influenza A/PR/8/34 (H1N1) virus activities of ten diarylheptanoids isolated from Alpinia officinarum were examined using the MTT method. The 50% inhibitory concentration of each diarylheptanoid examined was clearly lower than its 50% cytotoxic concentration determined by the MTT assay and/or maximum non-cytotoxic concentration (MNCC) determined by the morphological change of cells. In particular, the influenza virus was more susceptible to 7-(4''-hydroxy-3''-methoxyphenyl)-1-phenyl-4E-hepten-3-one (3) and (5S)-5-hydroxy-7-(4''-hydroxyphenyl)-1-phenyl-3-heptanone (8) than the other diarylheptanoids. Thus, all diarylheptanoids exhibited potential antiviral activity against influenza virus in vitro.
Sawamura
Rie
R
Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, Japan.
Sun
Yi
Y
Yasukawa
Ken
K
Shimizu
Tomomi
T
Watanabe
Wataru
W
Kurokawa
Masahiko
M
D032429:Alpinia / Q000737:chemistry*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D036381:Diarylheptanoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D013778:Tetrazolium Salts; D013844:Thiazoles
D000998:Antiviral Agents; D036381:Diarylheptanoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue