[The inhibitory effect of curcumin on the proliferation of HT-29 colonic cancer cell induced by deoxycholic acid].
OBJECTIVE
To assess the effect of curcumin on deoxycholic acid (DCA) induced cell proliferation in human HT-29 colon cancer cell line and to discuss its underlying anti-cancer mechanisms.


METHODS
The effect of curcumin on cell proliferation was tested in vitro with MTT assay. RT-PCR was applied to measure cyclooxygenase-2 (COX-2) mRNA transcription. Cellular immunochemical stain was applied to label COX-2 protein expression. Radio immunoassay was applied to assess prostaglandin E(2) (PGE(2)) level.


RESULTS
DCA increased cell proliferation, COX-2 mRNA expression and PGE(2) concentration of HT-29 cell line. Curcumin inhibited DCA-induced cell proliferation of HT-29 cell in a dose and time depended manner. Curcumin may inhibit DCA-induced COX-2 mRNA transcription at 6 hour with more than 5 micromol/L and at 12 hour with more than 0.5 micromol/L. However, Curcumin may decrease DCA-induced COX-2 expression and PGE(2) synthesis at 24 - 48 hour with more than 1 micromol/L and at 6-12 hour with more than 5 micromol/L.


CONCLUSIONS
Curcumin may inhibit HT-29 cell proliferation, COX-2 mRNA transcription, COX-2 protein expression and PGE(2) synthesis induced by DCA in HT-29 cell line. These data provide new insights into the mechanism of its anti-cancer properties.
Wang
Bang-mao
BM
Department of Gastroenterology, General Hospital, Tianjin Medical University, Tianjin 300052, China. gi.tmuh@sohu.com
Zhai
Chun-ying
CY
Fang
Wei-li
WL
Chen
Xin
X
Jiang
Kui
K
Wang
Yu-ming
YM
D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003840:Deoxycholic Acid; D015232:Dinoprostone / Q000096:biosynthesis; D019073:HT29 Cells; D006801:Humans
D003840:Deoxycholic Acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D003474:Curcumin; D015232:Dinoprostone