[Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells].
OBJECTIVE
To study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.


METHODS
HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.


RESULTS
HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.


CONCLUSIONS
Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.
Wu
Shi-Qing
SQ
Dept. of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatology Hospital, The Affiliated Stomatology Hospital of Southern Medical University, Guangzhou 510280, China.
Zheng
Jun-Fa
JF
Zeng
Shu-Guang
SG
Chen
Shao-Peng
SP
Xue
Guo-Chu
GC
Zhang
Jin-Cai
JC
D002478:Cells, Cultured; D066255:EGF Family of Proteins; D042783:Endothelial Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007136:Immunoglobulins; D053634:TYK2 Kinase; D014471:Umbilical Veins
D066255:EGF Family of Proteins; D007136:Immunoglobulins; D053634:TYK2 Kinase