Use of folded micromachined pillar array column with low-dispersion turns for pressure-driven liquid chromatography.
In this study, we show for the first time that the separation efficiency of a pillar array column under pressure-driven liquid chromatography (LC) conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. With the low-dispersion-turn geometry, a column with a length and width of 110 mm and 400 microm, respectively, could be fabricated on a 20 x 20 mm microchip. Under nonretained conditions, the solute bands obtained for fluorescent compounds remained almost unchanged even after passing through the low-dispersion turns; however, significant skewing of the solute bands was observed in the case of constant-radius turns. Two coumarin dyes were well resolved under reversed-phase conditions, and a maximum theoretical plate number of 8000 was obtained. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s. These results indicated that the separation efficiency of microchip chromatography could be significantly improved using a long separation channel with low-dispersion turns.
Aoyama
Chiaki
C
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Saeki
Akira
A
Noguchi
Masao
M
Shirasaki
Yoshitaka
Y
Shoji
Shuichi
S
Funatsu
Takashi
T
Mizuno
Jun
J
Tsunoda
Makoto
M
D002853:Chromatography, Liquid / Q000295:instrumentation*; D003374:Coumarins / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000302:isolation & purification; D007267:Injections; D046228:Microarray Analysis; D011312:Pressure
D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin