Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA.
Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis (TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine industry. Previously, we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA polymerase (RdRP) gene of TGEV with more than 95% of virus inhibition in vitro. In this study, inhibition of the TGEV replication by pEGFP-U6/P1 and pEGFP-U6/P2 was tested in mini-pigs. SPF mini-pigs at 25 days old were injected with the shRNA-expressing plasmids and then infected with TGEV. The results from the analyses of clinical signs, histopathology, indirect immunofluorescence (IIF) and RT-PCR show that the two shRNA-expressing plasmids could significantly decrease the quantity of TGEV in different organs and protect mini-pigs from TGEV infection. These findings illustrate the prospect for TGEV-specific shRNAs to be new anti-TGEV agents.
Zhou
Junfang
J
East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Science, 300 Jungong Road, Shanghai 200090, China.
Huang
Fen
F
Hua
Xiuguo
X
Cui
Li
L
Zhang
Wen
W
Shen
Yan
Y
Yan
Yijia
Y
Chen
Piren
P
Ding
Dezhong
D
Mou
Jing
J
Chen
Qi
Q
Lan
Daoliang
D
Yang
Zhibiao
Z
D000825:Animal Structures / Q000473:pathology / Q000821:virology; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D001688:Biological Products / Q000235:genetics / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005761:Gastroenteritis, Transmissible, of Swine / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use*; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors / Q000235:genetics; D013552:Swine; D013556:Swine, Miniature; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000502:physiology*; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D001688:Biological Products; D034741:RNA, Small Interfering; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase