[The influence of aminoglycoside antibiotics on the conformation of the unpairing loop adenine of avian leukosis virus RNA as revealed with 2-aminopurine fluorescence].
Fluorescent properties of 2-aminopurine included in the sequence RNA, are now used for the study of structural dynamics and local changes of structure retroviral RNA. We used 2-aminopurine for studying the conformational states of unpaired loop adenine of avian leukosis virus RNA upon the interaction with the aminoglycoside antibiotics. It was shown, that intensity of 2-aminopurine fluorescence for monomer hairpin RNA has greater value in comparison with both RNA dimers. Comparing the 2-aminopurine fluorescence of RNA dimers, it has found out that the intensity of fluorescence for extended dimer significantly lower than the kissing loop dimer RNA. This can be explained by the fact that the stacking contacts forms more compact structure of a loop in extended dimer concerning those in structure of kissing loop dimer. When the binding of aminogycoside antibiotics with kissing loop dimer RNA it was observed that only the tobramycin increases nearly three times the intensity of 2-aminopurine fluorescence. Results of work testify that it is possible to detect local changes in the complexes of retroviral RNA with ligands, using the fluorescence 2-aminopurine.
Shukshina
I Z
IZ
Miniat
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EG
D000617:Aminoglycosides / Q000737:chemistry*; D000632:Aminopyrine / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001354:Avian Leukosis Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012367:RNA, Viral; D000632:Aminopyrine