title: [Effect of nano-granule titanium films on synthesis of osteoblasts].
abstract: OBJECTIVE
  To compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility.
  
  
  METHODS
  Four different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 degrees C, 250 degrees C, 380 degrees C substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample(control group). The production of osteocalcin (OC) in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 degrees C group was the highest, and it showed significant differences with 380 degrees C, control group and blank group. In 250 degrees C group, the production of OC also showed significant differences with 380 degrees C, control group and blank group (P < 0.05).
  
  
  CONCLUSIONS
  Titanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Ba
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Yang
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Miao
mesh_terms: D000818:Animals; D010006:Osteoblasts; D015675:Osteocalcin; D051381:Rats; D013499:Surface Properties; D014025:Titanium
chemical_list: D015675:Osteocalcin; D014025:Titanium