[Improvement of the rapid identification method for H1 and H2 genotype of measles virus].
OBJECTIVE
To simplify the procesure of the rapid genotyping method of H1 and H2 genotypes of measles virus and make the identifying operation easily.


METHODS
To improve the reverse transcription nested-polymerase chain reaction (RT-nPCR) to RT-PCR by using the inner primer pair of the RT-nPCR, identify the genotype of the PCR product by using restriction fragment length polymorphism (RFLP) method, and also use the safer GeneFinder in electrophoresis instead of EB which maybe induce cancer.


RESULTS
3 strains measles virus of known H1 genotype got good positive results by the improved one-step RT-PCR and the PCR products were also cut well by Sal I. The improved method keep the sensitivity and specificity of the old method well.


CONCLUSIONS
The improved method of identifying H1 and H2 genotype is a genotyping method which shows more rapidly, simply and applicably.
Zhou
Jian-Hui
JH
Center for Disease Control and Prevention of Jilin Province, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Du
Zhan-Sen
ZS
Wang
Shuang
S
D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D008459:Measles virus / Q000235:genetics* / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral