Heat shock-induced three-dimensional-like proliferation of normal human fibroblasts mediated by pressed silk.
The aim of this study was to determine the optimal heat treatment conditions for enhancement of pressed silk-mediated 3D-like proliferation of normal human dermal fibroblasts, as well as to determine the responses to heat shock of cells and intracellular signaling pathways. The beginning of 3D-like pattern formation of cells was observed in the second week after the start of the experiment. The mean rates of beginning of 3D-like pattern formation by cells heat-treated at 40 masculineC and 43 masculineC for 10 min were significantly higher (3.2- and 8.6-fold, respectively) than that of untreated cells. We found that apoptosis had occurred in 7.5% and 50.0% of the cells at one week after heat treatment for 10 min at 43 masculineC and 45 masculineC, respectively. Western blot analysis demonstrated that phosphorylation of p38 MAPK and that of Hsp27 were markedly increased by heat treatment at 43 masculineC for 10 min. The results of an experiment using a p38 MAPK inhibitor and Hsp27 inhibitor suggest that activation of p38 MAPK by heat shock is associated with 3D-like cell proliferation and that Hsp27 contributes to the inhibition of apoptosis. The results of this study should be useful for further studies aimed at elucidation of the physiologic mechanisms underlying thermotherapy.
Hiragami
Fukumi
F
Department of Physical Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan.
Motoda
Hirotoshi
H
Department of Physical Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan.
Takezawa
Toshiaki
T
Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences, Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan.
Takabayashi
Chiyuki
C
Laboratory of New Silk Materials, National Institute of Agrobiological Sciences, Gouda 1-4-8, Okaya, Nagano 394-0021, Japan.
Inoue
Shigeki
S
Department of Physical Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan.
Wakatake
Yuji
Y
Department of Physical Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan.
Kano
Yoshio
Y
Department of Occupational Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan.
D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D047011:Silk / Q000737:chemistry* / Q000494:pharmacology; D013696:Temperature; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D055551:HSP27 Heat-Shock Proteins; D047011:Silk; D048051:p38 Mitogen-Activated Protein Kinases