title: Engineered transaminopeptidase, aminolysin-S for catalysis of peptide bond formation to give linear and cyclic dipeptides by one-pot reaction.
abstract: Aminopeptidase from Streptomyces thermocyaneoviolaceus NBRC14271 was engineered into transaminopeptidase and used to catalyze an aminolysis reaction to give linear and cyclic dipeptides from cost-effective substrates such as the ester derivatives of amino acids.
authors.affiliation: Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Usuki
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Uesugi
authors.forename: Jiro
authors.initials: J
authors.lastname: Arima
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Masaki
authors.initials: M
authors.lastname: Iwabuchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatanaka
mesh_terms: D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D004151:Dipeptides / Q000138:chemical synthesis* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D004151:Dipeptides; D010456:Peptides, Cyclic; D000626:Aminopeptidases