[Effect of nano-granule titanium films on synthesis of osteoblasts].
OBJECTIVE
To compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility.


METHODS
Four different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 degrees C, 250 degrees C, 380 degrees C substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample(control group). The production of osteocalcin (OC) in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay.


RESULTS
The production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 degrees C group was the highest, and it showed significant differences with 380 degrees C, control group and blank group. In 250 degrees C group, the production of OC also showed significant differences with 380 degrees C, control group and blank group (P < 0.05).


CONCLUSIONS
Titanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.
Ba
Kai
K
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Zhang
Jing
J
Wang
Hu
H
Liu
Yuan-Yuan
YY
Yang
Zhen-Yu
ZY
Li
Ming-Xia
MX
Li
Wei
W
Miao
Jing-Wei
JW
D000818:Animals; D010006:Osteoblasts; D015675:Osteocalcin; D051381:Rats; D013499:Surface Properties; D014025:Titanium
D015675:Osteocalcin; D014025:Titanium