Overexpression of the fragile histidine triad (FHIT) gene in inflammatory bowel disease.
OBJECTIVE
FHIT gene encodes human diadenosine triphosphate hydrolase involved in the regulation of cell cycle and nucleotide metabolism and is a candidate tumor suppressor gene.


OBJECTIVE
To investigate expression of FHIT gene at the mRNA and protein levels in sporadic inflammatory bowel disease (IBD).


METHODS
FHIT mRNA was quantified by the validated real-time PCR (QPCR) and FHIT protein was detected by immunohistochemistry (IHC) in mucosal biopsies of 139 ulcerative colitis (UC), 19 Crohn's disease (CD) and 37 control patients.


RESULTS
Significant FHIT gene overexpression was found in 78% of active UC but not in CD. IHC showed comparable results to QPCR.


CONCLUSIONS
The local up-regulation of FHIT gene and protein expression in active UC may represent an adequate response against inflammatory challenge of epithelial cell homeostasis and protect against DNA damage and cell cycle disturbances.
Wierzbicki
P M
PM
Department of Histology, Medical University of Gdansk, Gdansk, Poland. pwierzb@amg.gda.pl
Adrych
K
K
Kartanowicz
D
D
Wypych
J
J
Stanislawowski
M
M
Zwolinska-Wcislo
M
M
Celinski
K
K
Skrodzka
D
D
Godlewski
J
J
Korybalski
B
B
Smoczynski
M
M
Kmiec
Z
Z
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017931:DNA Primers; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011044:Poland; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D017931:DNA Primers; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase