Assessment and comparison of immunoregulatory activity of four hydrosoluble fractions of Angelica sinensisin vitro on the peritoneal macrophages in ICR mice.
Angelica sinensis (Oliv) Diels, a traditional oriental herbal medicine, is known to have immunostimulatory and antitumor effects. In this paper, four hydrosoluble fractions were obtained and purified from A. sinensis including: Angelica polysaccharide, oligosaccharides (Angelica oligosaccharide and Angelica sucrose) and Angelica total amino acid, named APS, AOS, AS, and TAA, respectively. Moreover, the immunomodulatory activity of these four fractions on murine peritoneal macrophages were assessed and compared from various aspects including cell proliferation, phagocytic activity, nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) release, induced nitric oxide synthetase (iNOS) and lysozyme (LZM) activity, intracellular adhesion molecule-1 (ICAM-1) expression on cell surface and Toll-like receptor 4 (TLR4) gene expression. Although APS exhibited the most obvious growth facilitative activity, AOS did better in activation of H(2)O(2) release; TAA exerted the best activation of phagocytic activity, production of NO and ICAM-1 expression. Furthermore, we found that the action mode of the four fractions was similar to that of LPS and the mechanism may be related to the upregulation of TLR4 mRNA. These results demonstrated that oligosaccharide and amino acid fractions, as well as polysaccharide fraction of Radix A. sinensis, could serve as immunomodulator and TLR4 as one of the immune receptors may play important role for this action.
Chen
Ying
Y
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Duan
Jin-ao
JA
Qian
Dawei
D
Guo
Jianming
J
Song
Bingsheng
B
Yang
Ming
M
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009113:Muramidase / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000032:analysis / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000378:metabolism
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D010936:Plant Extracts; D051197:Toll-Like Receptor 4; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D052247:Nitric Oxide Synthase Type II; D009113:Muramidase