title: [Determination of pseudoprodioscin in Dioscorea panthaica by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for the determination of pseudoprodioscin in Dioscorea panthaica and provide scientific basis for quality control of it and its preparation.
  
  
  METHODS
  The analysis was carried out on a Merck Purospher STAR RP-18e column eluted with acetonitrile and water as mobile phases in gradient mode. The detection wavelength was 203 nm, and the flow rate was 1.0 mL min(-1).
  
  
  RESULTS
  The pseudoprodioscin in D. panthaica extract was well separated. The linear range is between 0.0800-4.00 microg, r =0.9999. The average recovery and RSD are 99.9% and 2.3%, respectively.
  
  
  CONCLUSIONS
  This method for quantitation of pseudoprodioscin in D. panthaica is simple, accurate and reliable and can be used for the quality control of D. panthaica and its preparations.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Jing
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhang
authors.forename: An
authors.initials: A
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D004144:Diosgenin