title: [Effectiveness of a polymerase chain reaction using seminested and multiplex strategy for the identification of the three main bacteria responsible for meningitis in Burkina Faso].
abstract: A prospective study (from August 2006 to April 2007) was carried out with 214 cerebrospinal fluid samples with suspicion of bacterial meningitis. The aim of the study was to assess the effectiveness of the simultaneous detection of Neisseria meningitidis, Streptococcus sp. and Haemophilus influenzae using seminested polymerase chain reaction strategy. Among the 214 samples tested by both PCR and culture, the overall confirmation rate was 64% for PCR and 40.1% for culture (P = 2 x 10⁻⁶). Taking culture method as the standard reference, the overall sensitivity of PCR was 98.8% and specificity, 59.4%. The sensitivity of PCR was 100, 97.3 and 100% respectively for N. meningitidis, Streptococcus sp. and H. influenzae with respective specificities of 70, 93.2 and 97.2%. In conclusion, the seminested PCR strategy is a sensitive method and it can be implemented in the reference public health laboratories for an exhaustive microbiological surveillance of bacterial meningitis.
authors.affiliation: Université de Ouagadougou, 3, BP 7021 Ouagadougou 03,Burkina Faso. abdoulniki@yahoo.fr
authors.forename: A
authors.initials: A
authors.lastname: Nikièma
authors.forename: L
authors.initials: L
authors.lastname: Toé
authors.forename: G
authors.initials: G
authors.lastname: Adjami
authors.forename: R
authors.initials: R
authors.lastname: Ouédraogo Traoré
mesh_terms: D002050:Burkina Faso; D004269:DNA, Bacterial / Q000032:analysis; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid* / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial