Aquaporin expression dynamics in osmoregulatory tissues of Atlantic salmon during smoltification and seawater acclimation.
Osmotic balance in fish is maintained through the coordinated regulation of water and ion transport performed by epithelia in intestine, kidney and gill. In the current study, six aquaporin (AQP) isoforms found in Atlantic salmon (Salmo salar) were classified and their tissue specificity and mRNA expression in response to a hyperosmotic challenge and during smoltification were examined. While AQP-1a was generic, AQP-1b had highest expression in kidney and AQP-3 was predominantly found in oesophagus, gill and muscle. Two novel teleost isoforms, AQP-8a and -8b, were expressed specifically in liver and intestinal segments, respectively. AQP-10 was predominantly expressed in intestinal segments, albeit at very low levels. Transfer from freshwater (FW) to seawater (SW) induced elevated levels of intestinal AQP-1a, -1b and -8b mRNA, whereas only AQP-8b was stimulated during smoltification. In kidney, AQP-1a, -3 and -10 were elevated in SW whereas AQP-1b was reduced compared with FW levels. Correspondingly, renal AQP-1a and -10 peaked during smoltification in April and March, respectively, as AQP-1b and AQP-3 declined. In the gill, AQP-1a and AQP-3 declined in SW whereas AQP-1b increased. Gill AQP-1a and -b peaked in April, whereas AQP-3 declined through smoltification. These reciprocal isoform shifts in renal and gill tissues may be functionally linked with the changed role of these organs in FW compared with SW. The presence and observed dynamics of the AQP-8b isoform specifically in intestinal sections suggest that this is a key water channel responsible for water uptake in the intestinal tract of seawater salmonids.
Tipsmark
C K
CK
Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. ckt@biology.sdu.dk
Sørensen
K J
KJ
Madsen
S S
SS
D000064:Acclimatization / Q000235:genetics*; D000818:Animals; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D002712:Chlorides / Q000097:blood; D005618:Fresh Water; D005786:Gene Expression Regulation; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020317:Salmo salar / Q000097:blood / Q000235:genetics* / Q000502:physiology*; D012623:Seawater; D013997:Time Factors; D014882:Water-Electrolyte Balance / Q000235:genetics*
D020346:Aquaporins; D002712:Chlorides; D012333:RNA, Messenger