Terrimonas aquatica sp. nov., isolated from a freshwater spring.
A yellow-pigmented bacterial strain, designated RIB1-6(T), was isolated from a freshwater spring in Taiwan. Strain RIB1-6(T) was aerobic, Gram-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 10-37 °C, at pH 7-8 and with 0-1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain RIB1-6(T) belonged to the genus Terrimonas and its two closest neighbours were Terrimonas ferruginea ATCC 13524(T) and Terrimonas lutea DY(T) (16S rRNA gene sequence similarity 97.4 % and 93.5 %, respectively). Strain RIB1-6(T) contained iso-C(15 : 0) (33.4 %), iso-C(17 : 0) 3-OH (18.2 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 14.7 %) and iso-C(15 : 1) (11.5 %) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The DNA G+C content of strain RIB1-6(T) was 47.3&emsp14;mol%. On the basis of the genotypic and phenotypic data, strain RIB1-6(T) represents a novel species in the genus Terrimonas, for which the name Terrimonas aquatica sp. nov. is proposed. The type strain is RIB1-6(T) (=BCRC 17941(T)=LMG 24825(T)).
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Cho
Nian-Tsz
NT
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka State, India.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7