Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria.
BACKGROUND
We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.


METHODS
Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.


RESULTS
By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.


CONCLUSIONS
This is the first study demonstrated the occurrence of SHV-12 in Nigeria.
Kasap
Murat
M
Tibbi Biyoloji AD, KOU Tip Fakultesi, Izmit, Turkey. mkasap@vt.edu
Fashae
Kayode
K
Torol
Sinem
S
Kolayli
Fetiye
F
Budak
Fatma
F
Vahaboglu
Haluk
H
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial / Q000235:genetics; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009549:Nigeria; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases