Effect of bee venom on transforming growth factor-beta1-treated hepatocytes.
Bee venom (BV) has been used as treatment against a wide variety of ailments, including inflammatory diseases. Various studies have demonstrated anti-inflammatory and anticancer effects of BV. Transforming growth factor (TGF)-beta1 induces hepatocyte apoptosis via the mitochondrial permeability transition. However, there is no evidence or information regarding the antiapoptotic effect of BV on hepatocytes. The authors investigated the antiapoptotic effect of BV on TGF-beta1-treated hepatocytes. The results showed significant protection from DNA damage by BV treatment compared to corresponding TGF-beta1-treated hepatocytes without BV. BV suppressed TGF-beta1-induced activation of the bcl-2 family and caspase family of proteins, which resulted in inhibition of poly ADP-ribose polymerase (PARP) cleavage. Furthermore, BV is not cytotoxic in the low concentrations used in this study. Low concentrations of BV potently suppress the apoptotic response in TGF-beta1-treated hepatocytes; therefore, BV may have therapeutic potential for the treatment of liver diseases.
Park
Jung-Hyun
JH
Department of Rehabilitation Medicine, Eulji University Hospital, Daejeon, South Korea.
Kim
Kyung-Hyun
KH
Kim
Soo-Jung
SJ
Lee
Woo-Ram
WR
Lee
Kwang-Gill
KG
Park
Ji-Hyun
JH
Park
Kwan-Kyu
KK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001514:Bee Venoms / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005562:Formazans / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D001514:Bee Venoms; D005562:Formazans; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D013778:Tetrazolium Salts; D016212:Transforming Growth Factor beta; C079782:MTT formazan; D011065:Poly(ADP-ribose) Polymerases