Dimethoate-induced oxidative stress in human erythrocytes and the protective effect of vitamins C and E in vitro.
Organophosphorus insecticides may induce oxidative stress leading to the generation of free radicals and alteration in the antioxidant system. The aim of this study was to examine the potency of Dimethoate (Dim) to induce oxidative stress response in human erythrocyte in vitro and the role of Vitamins C (Vit C) and E (Vit E) in alleviating the cytotoxic effects. Erythrocytes were divided into three groups. The first group, erythrocytes were incubated for 4 h at 37 °C with different concentrations (0, 20, 40, 60, 80, and 100 mM) of Dim. The second and third groups were preincubated with Vit C or Vit E, respectively, for 30 min and followed by Dim incubation for 4 h at 37 °C. Following in vitro exposure, Dim caused a significant increase in malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) in erythrocytes at different concentrations. Vit E or Vit C pretreated erythrocytes showed a significant protection against the cytotoxic effects inducted by Dim on the studied parameters. In conclusion, antioxidant Vit E and C could protect against Dim-induced oxidative stress by decreasing lipid peroxidation and hyperactivity of SOD and CAT in human erythrocytes.
Abdallah
Fatma Ben
FB
Human Pathologies and Oxidative Stress Unit, Higher Institute of Biotechnology of Sfax, Tunisia. fatma_isbs@yahoo.fr
Gargouri
Bochra
B
Bejaoui
Hafedh
H
Lassoued
Saloua
S
Ammar-Keskes
Leila
L
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004117:Dimethoate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D007306:Insecticides; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D004117:Dimethoate