title: Hydrophilic biopolymer grafted on poly(dimethylsiloxane) surface for microchip electrophoresis.
abstract: A novel covalent strategy was developed to modify the poly(dimethylsiloxane) (PDMS) surface. Briefly, dextran was selectively oxidized to aldehyde groups with sodium periodate and subsequently grafted onto amine-functionalized PDMS surface via Schiff base reaction. As expected, the coated PDMS surface efficiently prevented the biomolecules from adsorption. Electro-osmotic flow (EOF) was successfully suppressed compared with that on the native PDMS microchip. Moreover, the stability of EOF was greatly enhanced and the hydrophilicity of PDMS surface was also improved. To apply thus-coated microchip, the separation of peptides, protein and neurotransmitters was investigated in detail. For comparison, these analytes were also measured on the native PDMS microchips. The results demonstrated that these analytes were efficiently separated and detected on the coated PDMS microchips. Furthermore, the relative standard deviations of their migration times for run-to-run, day-to-day, and chip-to-chip reproducibilities were in the range of 0.6-2.7%. In addition, the coated PDMS microchips showed good stability within 1 month.
authors.affiliation: School of Chemistry and Environmental Science, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Jianshe Road, Xinxiang, Henan 453007, China.
authors.forename: Jiu-Ju
authors.initials: JJ
authors.lastname: Feng
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010504:Periodic Acid / Q000737:chemistry; D010544:Peroxidases / Q000032:analysis; D013499:Surface Properties
chemical_list: D001704:Biopolymers; D004129:Dimethylpolysiloxanes; D018377:Neurotransmitter Agents; D010455:Peptides; D010504:Periodic Acid; C009288:metaperiodate; D010544:Peroxidases