title: Inducing stroke in aged, hypertensive, diabetic rats.
abstract: Animal models of ischemic stroke often neglect comorbidities common in patients. This study shows the feasibility of inducing stroke by 2 h of thread occlusion of the middle cerebral artery in aged (56 week old) spontaneously hypertensive rats (SHRs) with both acute (2 weeks) and chronic (36 weeks) diabetes. After modifying the streptozotocin dosing regimen to ensure that old SHRs survived the induction of diabetes, few died after induction of stroke. Induction of stroke is feasible in rats with multiple comorbidities. Inclusion of such comorbid animals may improve translation from the research laboratory to the clinic.
authors.affiliation: National Stroke Research Institute and University of Melbourne Department of Medicine, Austin Health, Heidelberg, Victoria, Australia.
authors.forename: Sarah S J
authors.initials: SS
authors.lastname: Rewell
authors.forename: John A
authors.initials: JA
authors.lastname: Fernandez
authors.forename: Susan F
authors.initials: SF
authors.lastname: Cox
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Spratt
authors.forename: Lisa
authors.initials: L
authors.lastname: Hogan
authors.forename: Elena
authors.initials: E
authors.lastname: Aleksoska
authors.forename: Leena
authors.initials: L
authors.lastname: van Raay
authors.forename: Gabriel T
authors.initials: GT
authors.lastname: Liberatore
authors.forename: Peter E
authors.initials: PE
authors.lastname: Batchelor
authors.forename: David W
authors.initials: DW
authors.lastname: Howells
mesh_terms: D000818:Animals; D015897:Comorbidity; D003921:Diabetes Mellitus, Experimental / Q000453:epidemiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000503:physiopathology*; D051381:Rats; D011918:Rats, Inbred SHR; D020521:Stroke / Q000453:epidemiology / Q000401:mortality / Q000503:physiopathology*