title: [Construction of the hemagglutinin-2-deficient mutant of Porphyromonas gingivalis].
abstract: OBJECTIVE
  To construct and identify the Porphyromonas gingivalis(Pg)ATCC33277 hemagglutinin-2(HA-2)-deficient mutant.
  
  
  METHODS
  The genomic DNA of Pg was isolated from PgATCC33277. The up/down stream genes of HA-2-HA(u), HA(l) were amplified by PCR, and inserted into pSY118 separately which contains a 2.1 kb antibiotic resistance ermF-ermAM cassette. The resultant recombinant plasmid-pSY118-HA was linearized as the gene targating fragment HA-ermF-ermAM and used in the electroporation of PgATCC33277. The Pg HA-2-deficient mutant was screened by allelic exchange. The test of aggregation of red blood cells was used to investigate the function change between PgHA2-deficient mutant and the wild type of PgATCC33277.
  
  
  RESULTS
  The PgHA-2-deficient mutant was identified by PCR. The ability of Pg HA-2-deficient mutant to aggregate red blood cell was significantly decreased compared with the wild type.
  
  
  CONCLUSIONS
  HA-2-deficient mutant of Pg ATCC33277 was constructed successfully, which lays a foundation for further study of its biological function.
authors.affiliation: Beijing Institute for Dental Research, Capital Medical University School of Stomatology, Beijing 100050, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.forename: Qiu-bo
authors.initials: QB
authors.lastname: Yang
authors.forename: Sheng-hui
authors.initials: SH
authors.lastname: Yang
mesh_terms: D000483:Alleles; D004903:Erythrocyte Aggregation; D006388:Hemagglutinins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics*
chemical_list: D006388:Hemagglutinins