New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures.
Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d(3)-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.
El Tahchy
Anna
A
Groupe S.U.C.R.E.S., UMR 7565 CNRS-Nancy-Université, Nancy-Vandoeuvre, France.
Boisbrun
Michel
M
Ptak
Agata
A
Dupire
François
F
Chrétien
Françoise
F
Henry
Max
M
Chapleur
Yves
Y
Laurain-Mattar
Dominique
D
D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D019668:Liliaceae / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D046509:Tissue Culture Techniques
D047151:Amaryllidaceae Alkaloids; D010636:Phenols; D003903:Deuterium