title: Expression of collagen types I and II on articular cartilage in a rat knee contracture model.
abstract: The purpose of our study was to clarify the expression patterns of collagen types I and II on articular cartilage after immobilization in a rat knee contracture model in 3 specific areas (noncontact area, transitional area, contact area). The unilateral knee joints of adult male rats were rigidly immobilized at 150 degrees of flexion using screws and a rigid plastic plate. Sham-operated animals had holes drilled in the femur and the tibia and screws inserted but were not plated. The expression patterns of collagen types I and II in each area were evaluated by in situ hybridization (ISH), immunohistochemistry (IHC), and quantitative real-time polymerase chain reaction (qPCR). The expression of collagen type II in the noncontact area was decreased by ISH but appeared unchanged when examined by IHC. In the transitional and contact areas, the expression of collagen type II was initially shown to have decreased and then increased at the hypertrophic chondrocytes by ISH but appeared decreased by IHC. Quantitative PCR revealed the decreased expression of type II collagen in the contact area. Immunostaining of collagen type I was increased at the noncontact area and transitional areas. Alterations of collagen types I and II expression may also affect the degeneration of articular cartilage after immobilization and the changes were different in the three areas.
authors.affiliation: Department of Orthopaedic Surgery, Tohoku University School of Medicine, Sendai; Takeda General Hospital, Aizuwakamatsu, Japan. hagi@mail.tains.tohoku.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: Akira
authors.initials: A
authors.lastname: Ando
authors.forename: Eiichi
authors.initials: E
authors.lastname: Chimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takahashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sasano
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Onoda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Suda
authors.forename: Eiji
authors.initials: E
authors.lastname: Itoi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001860:Bone Plates; D001863:Bone Screws; D002358:Cartilage, Articular / Q000378:metabolism / Q000503:physiopathology*; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics* / Q000378:metabolism; D003286:Contracture / Q000150:complications*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D019637:Orthopedic Procedures / Q000295:instrumentation / Q000379:methods; D020370:Osteoarthritis, Knee / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000295:instrumentation / Q000379:methods
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024043:Collagen Type II; D012333:RNA, Messenger