Differential effects of cream, glucose, and orange juice on inflammation, endotoxin, and the expression of Toll-like receptor-4 and suppressor of cytokine signaling-3.
OBJECTIVE
We have recently shown that a high-fat high-carbohydrate (HFHC) meal induces an increase in plasma concentrations of endotoxin (lipopolysaccharide [LPS]) and the expression of Toll-like receptor-4 (TLR-4) and suppresser of cytokine signaling-3 (SOCS3) in mononuclear cells (MNCs) in addition to oxidative stress and cellular inflammation. Saturated fat and carbohydrates, components of the HFHC meal, known to induce oxidative stress and inflammation, also induce an increase in LPS, TLR-4, and SOCS3.


METHODS
Fasting normal subjects were given 300-calorie drinks of either glucose, saturated fat as cream, orange juice, or only water to ingest. Blood samples were obtained at 0, 1, 3, and 5 h for analysis.


RESULTS
Indexes of inflammation including nuclear factor-kappaB (NF-kappaB) binding, and the expression of SOCS3, tumor necrosis factor-alpha (TNF-alpha), and interleukin (IL)-1beta in MNCs, increased significantly after glucose and cream intake, but TLR-4 expression and plasma LPS concentrations increased only after cream intake. The intake of orange juice or water did not induce any change in any of the indexes measured.


CONCLUSIONS
Although both glucose and cream induce NF-kappaB binding and an increase in the expression of SOCS3, TNF-alpha, and IL-1beta in MNCs, only cream caused an increase in LPS concentration and TLR-4 expression. Equicaloric amounts of orange juice or water did not induce a change in any of these indexes. These changes are relevant to the pathogenesis of atherosclerosis and insulin resistance.
Deopurkar
Rupali
R
Division of Endocrinology, Diabetes, and Metabolism, State University of New York at Buffalo and Kaleida Health, Buffalo, New York, USA.
Ghanim
Husam
H
Friedman
Jay
J
Abuaysheh
Sanaa
S
Sia
Chang Ling
CL
Mohanty
Priya
P
Viswanathan
Prabhakar
P
Chaudhuri
Ajay
A
Dandona
Paresh
P
D000209:Acute-Phase Proteins; D000328:Adult; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood; D032084:Citrus sinensis; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage / Q000097:blood; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000276:immunology; D007328:Insulin / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood; D008562:Membrane Glycoproteins / Q000097:blood; D008875:Middle Aged; D008892:Milk; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D019518:Postprandial Period / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D001786:Blood Glucose; D002352:Carrier Proteins; D016207:Cytokines; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005947:Glucose; D007328:Insulin; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D012333:RNA, Messenger; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D051197:Toll-Like Receptor 4; D000099231:Lipopolysaccharide-Binding Protein; C106644:SOCS3 protein, human; C495345:TLR4 protein, human