Nrf3-deficient mice are not protected against acute lung and adipose tissue damages induced by butylated hydroxytoluene.
We found that both wild type and Nrf3 (NF-E2-related factor 3) deficient mice are sensitive to BHT single administration exhibiting respiratory distress and considerably lose body weight following treatment. At time of sacrifice, the BHT-treated Nrf3-/- mice had lost significantly more body weight than their WT counterparts. In the lung, transcript levels of the transcription factors Nrf1, Nrf2 and Nrf3 were differentially regulated by BHT treatment. In addition, genes implicated in adipogenesis were repressed following BHT exposure in the white adipose tissue. Together, our data provide the first evidence that BHT exposure not only affects lung function but also leads to impaired adipogenesis in adipocytes.
Chevillard
Grégory
G
Lady Davis Institute for Medical Research, Department of Medicine, Division of Experimental Medicine, McGill University, Montreal, Quebec, Canada.
Nouhi
Zaynab
Z
Anna
Derjuga
D
Paquet
Marilène
M
Blank
Volker
V
D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D051265:NF-E2-Related Factor 1 / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000235:genetics
D050976:Basic-Leucine Zipper Transcription Factors; D051265:NF-E2-Related Factor 1; D051267:NF-E2-Related Factor 2; C088357:NFE2L1 protein, human; C495636:Nfe2l2 protein, mouse; C485120:Nrf3 protein, mouse; D002084:Butylated Hydroxytoluene; D002374:Catalase