title: [First isolation and detection of multiple clones of vancomycin-resistant enterococci in the pediatric unit of Van Yuzuncu Yil University Turkey].
abstract: Upon isolation of the first vancomycin resistant enterococcus (VRE) from the urine sample of a nine months old patient in pediatric unit of Van Yuzuncu Yil University Hospital (located in eastern part of Turkey), we aimed to search for the presence of VRE isolates in the unit, to determine the resistance genotypes and to evaluate the clonal relationships among isolates. A total of 28 rectal swabs and 28 skin swabs from the patients, 12 skin swabs from the staff giving care to the patients, 15 skin swabs from the mothers of the patients and 96 environmental samples from the pediatric unit were screened. Antibiotic susceptibilities were tested and the resistance genotypes were determined. Molecular typing of the isolates was performed using pulsed-field gel electrophoresis (PFGE). Apart from the first case, 13 more VRE isolates, one being a clinical isolate from the urine of a patient and 12 isolates from the screening samples (8 rectal swabs, one skin swab and three swabs from patients' beds) were obtained. All of the isolates were identified as Enterococcus faecium with similar antibiotic susceptibility patterns. VanA gene was present in all of the isolates. PFGE demonstrated two major clones and five clones closely related with the major ones. This was the first VRE isolation and colonization reported in our region. The isolates belonged to more than one clone. Currently, VRE did not seem to be a significant pathogen in Turkey, however, there may be an underestimation of the problem and continuous surveillance studies should be undertaken in every region.
authors.affiliation: Yüzüncü Yil Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Van.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Güdücüoğlu
authors.forename: Elif
authors.initials: E
authors.lastname: Aktaş
authors.forename: Füsun
authors.initials: F
authors.lastname: Beğendik Cömert
authors.forename: Kumru
authors.initials: K
authors.lastname: Aygül
authors.forename: Nagihan
authors.initials: N
authors.lastname: Ozlü
authors.forename: Sanem
authors.initials: S
authors.lastname: Baykal
authors.forename: Mustafa
authors.initials: M
authors.lastname: Berktaş
authors.forename: Abdullah
authors.initials: A
authors.lastname: Ceylan
mesh_terms: D000293:Adolescent; D001426:Bacterial Proteins / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012007:Rectum / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D014421:Turkey; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases