An Evaluation of the Effectiveness of a Semi-automatic Metaphase Locating and On-screen Karyotyping System.
Karyotyping is currently the "gold standard" test for the detection of human chromosome abnormalities. Over the past 40 years, changes in techniques have improved the band definition of chromosomes; however, very little has changed with respect to improvements through automation. In this study, we compare chromosome analysis by traditional microscopy with semi-automatic karyotyping using robotic equipment from MetaSystems (Altlussheim, Germany). Analysis using MetaSystems was significantly quicker than using the microscope with an average reduction in analysis time of 26.5 minutes; for the average analyst, this equates to a reduction of 27 percent. Analysis checking times using MetaSystems showed even greater improvement with an average reduction in checking time of 11.4 minutes; for the average checker, this equates to a reduction of 48 percent. The MetaSystems semi-automatic karyotyping equipment offers increased throughput of cases for karyotype analysis while maintaining accuracy.
May
Philippa C
PC
Cytogenetics Department, Guy's Hospital, Great Maze Pond, London.
Ogilvie
Caroline Mackie
CM
Mohammed
Shehla
S
Docherty
Zoe
Z
Hall
Richard Peter
RP