The rate of loss of T-tubules in cultured adult ventricular myocytes is species dependent.
In this study, we compared the rate of detubulation of adult mouse and rat ventricular myocytes over a 72 h culture period. The T-tubule density was measured in the following two ways: (i) as whole-cell capacitance in voltage-clamped myocytes relative to cell area; and (ii) using di-8-ANEPPS staining and confocal microscopy. In adult rat ventricular myocytes, whole-cell capacitance/area was significantly reduced from 47 +/- 3 fF microm(2) (mean +/- s.e.m.; n = 16) in freshly isolated (control) cells to 36 +/- 2 fF microm(2) (n = 20) after 72 h in culture. The T-tubular density, as assessed optically using di-8-ANEPPS staining, at 48 h was significantly reduced to 70 +/- 7% (n = 14) compared with control cells. The T-tubular density was further reduced after 72 h in culture to 43 +/- 7% (n = 10) compared with control cells. In contrast, in mouse myocytes neither whole-cell capacitance relative to cell area nor optical assessment of T-tubules showed any significant reduction in capacitance/cell area or T-tubule density after 72 h of culture. Expression of caveolin-3 (CAV-3) (a marker of T-tubule development) was also measured, and a significant reduction was observed in CAV-3 expression in rat myocytes at 48 (80 +/- 5.5%; n = 6) and 72 h (66 +/- 9.5%; n = 6) compared with control cells. The expression of CAV-3 in mouse myocytes was not significantly reduced even at 72 h. When rat ventricular myocytes were paced in culture for 72 h they exhibited no significant improvement in T-tubule density or CAV-3 expression compared with non-paced cultured cells. In rat myocytes, sarcomere length shortening was significantly reduced in myocytes cultured for 48 (4.96 +/- 0.72%; n = 26) and 72 h (4.32 +/- 0.80%; n = 26) compared with freshly isolated cells (7.12 +/- 0.56%; n = 18). Mouse myocytes, after 24 h in culture, were unable to follow external pacing. These results suggest that detubulation in quiescent culture is slower in the mouse than the rat and that this loss of T-tubules profoundly affects excitation-contraction coupling in rat myocytes.
Pavlović
Davor
D
Cardiovascular Division, King's College London, The Rayne Institute, St Thomas' Hospital, London SE1 7EH, UK.
McLatchie
Linda M
LM
Shattock
Michael J
MJ
D000818:Animals; D051244:Caveolin 3 / Q000096:biosynthesis; D002478:Cells, Cultured; D025802:Electric Capacitance; D022062:Electrophysiologic Techniques, Cardiac; D006352:Heart Ventricles / Q000166:cytology; D051379:Mice; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011726:Pyridinium Compounds; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D051244:Caveolin 3; D011726:Pyridinium Compounds