Use of a redox-sensing GFP (c-roGFP1) for real-time monitoring of cytosol redox status in Arabidopsis thaliana water-stressed plants.
Using Arabidopsis plants transformed with a redox-sensing green fluorescent protein targeted to the cytosol (c-roGFP1), we have demonstrated, in real time, measurements of reversible changes of redox status in the cytosol of plants subjected to a gradual water-stress, followed by re-watering. Plants sensed water stress, and changed the redox potential of their cytosol to a more oxidized value after a gradually-imposed water stress. Small variations in the cytosol redox potential and ascorbate (AA) values suggest that this parameter was tightly regulated. The re-watering was paralleled by a return of water stress, redox potential and ascorbate to initial values, showing the reversibility of water stress and its consequences.
Jubany-Mari
T
T
Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain. tanajubany@ub.edu
Alegre-Batlle
L
L
Jiang
K
K
Feldman
L J
LJ
D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D001205:Ascorbic Acid / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D003681:Dehydration / Q000378:metabolism*; D003683:Dehydroascorbic Acid / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species
D017382:Reactive Oxygen Species; D049452:Green Fluorescent Proteins; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid