[Cytophotometric expressions of CASPASE-3 and CD-34 in breast cancer].
OBJECTIVE
Describe, correlate and compare the expression of the tumor markers CD 34 (angiogenesis) and caspase-3 (apoptosis) in invasive breast adenocarcinoma, through image cytometry with the system SAMBA4000.


METHODS
Twenty-two cases of invasive breast adenocarcinoma from paraffin-embedded archival tissue, and after specific prepare, fifteen cases presented a satisfactory lecture with SAMBA4000 and could, finally, be evaluated by the software IMMUNO(R) (n = 15). The parameters analysed were the label index - in percentage, indicating the marked surface - and the optical density, in pixels - indicator of the marker intensity. The results were tabulated and expressed in averages, mediums, minimum and maximum values. The statistic analysis was realized by the Shapiro-Wilkins, Student test, Pearson's and Spearman's correlation, with statistic significance for values from p < 0,05.


RESULTS
There was no data normality for the label index CD34 (p= 0,019), there was normality in the analysis of the optical density of both markers and label index of the marker Caspase-3. There was no difference relating to the average of the index marker and the optical density when they were compared.


CONCLUSIONS
There was a tendency to correlate the label index and the optical density of the tumor marker caspase-3, the same did not occur with the tumor marker CD 34. Other analysis did not show any correlation between the two studied markers. Other studies involving theses two cell processes are needed to extend the knowledge of the cancer biomolecular mechanic and to permit new diagnostic and therapeutic strategies.
dos Passos
Fábio Santana
FS
Programa de Pós-Graduação em Princípios da Cirurgia, Hospital Universitário Evangélico de Curitiba, Faculdade Evangélica do Paraná, Curitiba, PR - BR.
Cuenca
Ronaldo Máfia
RM
Malafaia
Osvaldo
O
Ribas-Filho
Jurandir Marcondes
JM
Czeczko
Nicolau Gregori
NG
Nassif
Paulo Afonso Nunes
PA
Ribas
Carmen Austrália Paredes Marcondes
CA
D018952:Antigens, CD34 / Q000032:analysis* / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000378:metabolism*; D053148:Caspase 3 / Q000032:analysis* / Q000096:biosynthesis*; D003592:Cytophotometry; D005260:Female; D006801:Humans
D018952:Antigens, CD34; D053148:Caspase 3