Bioconversion of 2,6-dimethylpyridine to 6-methylpicolinic acid by Exophiala dermatitidis (Kano) de Hoog DA5501 cells grown on n-dodecane.
Alkane-assimilating microorganisms were isolated from enrichment cultures using n-octane, n-dodecane, n-hexadecane, or pristane (2,6,10,14-tetramethylpentadecane) as a sole carbon source to find microbial catalysts oxidizing methyl groups of 2,6-dimethylpyridine. The cells of Exophiala dermatitidis (Kano) de Hoog DA5501, an n-dodecane-assimilating fungus, oxidized a single methyl group of 2,6-dimethylpyridine to produce 6-methylpicolinic acid (6-methylpyridine-2-carboxylic acid) without the formation of dipicolinic acid (pyridine-2,6-dicarboxylic acid); 67 mM 6-methylpicolinic acid (9.2 g/l) accumulated with a molar conversion yield of 89% by 54-h incubation. The fungus cells also oxidized the methyl group of 2,6-dimethylpyrazine and 2,4,6-trimethylpyridine regioselectively.
Yoshida
Toyokazu
T
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu, Japan. toyosida@gifu-u.ac.jp
Sada
Yuki
Y
Nagasawa
Toru
T
D000473:Alkanes / Q000378:metabolism*; D001711:Biotransformation; D005093:Exophiala / Q000254:growth & development* / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D010848:Picolinic Acids / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D013997:Time Factors
D000473:Alkanes; D010848:Picolinic Acids; D011725:Pyridines; C007548:n-dodecane