title: [Sequence analysis of hemin-binding peptide derived from recombinant hemagglutinin-2 of Porphyromonas gingivalis].
abstract: OBJECTIVE
  To determine the sequence of the active peptide derived from recombinant hemagglutinin (rHA-2) of Porphyromonas gingivalis (Pg).
  
  
  METHODS
  The HA-2 gene from PgATCC33277 was cloned, expressed in Escherichia coli (Ec) BL21 (DE3), and purified. The purified recombinant protein was evaluated for its ability to bind hemin-linked agarose. The active peptide was subjected to endoproteinase-mediated sequence analysis.
  
  
  RESULTS
  The protein expressed in Ec BL21 (DE3) was identified as PgHA-2 by plasmid sequence analysis, Western blotting, and mass spectrometry. The recombinant protein was confirmed functional by its ability to bind hemin. The sequence of the active peptide of rHA-2 was determined to be DHYAVMISKTGTNAG.
  
  
  CONCLUSIONS
  The availability of sequence of the active peptide of rHA-2 provides a foundation for the development of immunoprophylactic and therapeutic agents against this human pathogen.
authors.affiliation: Beijing Institute for Dental Research, Capital Medical University School of Stomatology, Beijing 100050, China. qiuboyang2003@163.com
authors.forename: Qiu-bo
authors.initials: QB
authors.lastname: Yang
authors.forename: Fei-yan
authors.initials: FY
authors.lastname: Yu
authors.forename: Sheng-hui
authors.initials: SH
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000235:genetics; D020539:Sequence Analysis, Protein
chemical_list: D001426:Bacterial Proteins; D006388:Hemagglutinins