Engineered transaminopeptidase, aminolysin-S for catalysis of peptide bond formation to give linear and cyclic dipeptides by one-pot reaction.
Aminopeptidase from Streptomyces thermocyaneoviolaceus NBRC14271 was engineered into transaminopeptidase and used to catalyze an aminolysis reaction to give linear and cyclic dipeptides from cost-effective substrates such as the ester derivatives of amino acids.
Usuki
Hirokazu
H
Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan.
Uesugi
Yoshiko
Y
Arima
Jiro
J
Yamamoto
Yukihiro
Y
Iwabuchi
Masaki
M
Hatanaka
Tadashi
T
D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D004151:Dipeptides / Q000138:chemical synthesis* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*
D004151:Dipeptides; D010456:Peptides, Cyclic; D000626:Aminopeptidases