title: Establishment of long-term monolayer cultures of somatic cells from human fetal testes and expansion of peritubular myoid cells in the presence of androgen.
abstract: The somatic (Sertoli cell (SC), Leydig cell (LC), and peritubular myoid (PTM) cell) cells play key roles in development of the fetal testis. We established monolayer cultures from second trimester human testes and investigated the pattern of expression of cell-lineage characteristic mRNAs. Expression of some SC-associated genes (SRY, SOX9, WT1, GATA4, and SF1) was detectable up to and including passage 3 (P3), while others (anti-Müllerian hormone; desert hedgehog) present prior to dissociation were not expressed in the cultured cells. Transcripts encoding the androgen receptor were expressed but addition of dihydrotestosterone (DHT) had no impact on expression of mRNAs expressed in SC or LC. Total concentrations of mRNAs encoding smooth muscle actin (ACTA2) and desmin increased from P1 to P3; an increasing proportion of the cells in the cultures were immunopositive for ACTA2 consistent with proliferation/differentiation of PTM cells. In conclusion, somatic cell monolayer cultures were established from human fetal testes; these cultures could form the basis for future studies based on isolation of purified populations of somatic cells and manipulation of gene expression that is difficult to achieve with organ culture systems. Our results suggest that fetal SC do not maintain a fully differentiated phenotype in vitro, yet PTM (ACTA2 positive) cells readily adapt to monolayer culture conditions in the presence of DHT. This culture system provides an opportunity to study the impact of regulatory factors on gene expression in PTM cells, a population thought to play a key role in mediating androgen action within the developing testis.
authors.affiliation: MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, Edinburgh EH16 4TJ, UK.
authors.forename: Gillian
authors.initials: G
authors.lastname: Cowan
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Childs
authors.forename: Richard A
authors.initials: RA
authors.lastname: Anderson
authors.forename: Philippa T K
authors.initials: PT
authors.lastname: Saunders
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000502:physiology; D006801:Humans; D008297:Male; D009095:Mullerian Ducts / Q000166:cytology / Q000196:embryology / Q000502:physiology; D009928:Organ Specificity; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D012708:Sertoli Cells / Q000166:cytology* / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D054928:Validation Studies as Topic
chemical_list: D000728:Androgens; D015415:Biomarkers