Quantitation of argatroban in plasma using liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS).
The following chapter describes a method to measure argatroban in plasma samples using ultra-performance liquid chromatography combined with electrospray positive ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Samples are pre-treated using methanol containing the internal standard (IS) diclofenac. The plasma extracts are dried under a stream of nitrogen. The residue is reconstituted in ammonium acetate-formic acid-water. The reconstituted plasma is injected into the UPLC-ESI-MS/MS. Argatroban and diclofenac show similar retention times in plasma. Quantification of argatroban in the samples is made by multiple reaction monitoring using the hydrogen adduct mass transitions, from a seven-point calibration curve.
Molinaro
Ross J
RJ
Department of Pathology and Laboratory Medicine, Emory University School of Medicine and Emory Healthcare Systems, Atlanta, GA, USA.
D001120:Arginine / Q000031:analogs & derivatives; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010875:Pipecolic Acids / Q000097:blood*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013449:Sulfonamides; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010875:Pipecolic Acids; D013449:Sulfonamides; D001120:Arginine; C031942:argatroban