title: [Effects and mechanisms of 5-aza-2'-deoxycytidine on endometrial cancer cell.].
abstract: OBJECTIVE
  To investigate the effects and mechanisms of 5-aza-2'-deoxycytidine (5-Aza-CdR) on endometrial cancer cell.
  
  
  METHODS
  In vitro experiments of 5-Aza-CdR were done using human endometrial cancer cell line HEC-1B. Evaluation of cellular proliferation and apoptosis was ascertained respectively using trypan blue exclusion and flow cytometry. RT-PCR and methylation specific PCR(MSP) was done to detect the expression of RASSF1A mRNA and methylation status of RASSF1A promoter of HEC-1B cell line.
  
  
  RESULTS
  (1) The status of cellular growth and apoptosis of HEC-1B cell line: the growth inhibition effects of 5-Aza-CdR on HEC-1B cell line were both concentration-dependent (P < 0.01) and time-dependent (P < 0.01), as well as the apoptosis rate of HBC-1-B cell line depended on the dose of 5-Aza-CdR obviously (P < 0.01). (2) The expression of RASSF1A mRNA of HEC-1B cell line: RASSF1A mRNA was expressed in HEC-1B cell after 5-Aza-CdR treatment, but it was undetectable before the treatment. In the groups with different concentration of 5-Aza-CdR (0.05, 0.1, 1, 5, 10 nmol/ml), the expression of RASSF1A mRNA was respectively 0.074 +/- 0.004, 0.105 +/- 0.004, 0.167 +/- 0.006, 0.334 +/- 0.005, 0.484 +/- 0.007, which were remarkably different from the group without 5-Aza-CdR(the expression of RASSF1A mRNA was 0; P < 0.01). (3) The hypermethylation of RASSF1A promoter of HEC-1B cell line: the hypermethylation of RASSF1A promoter was detected in HEC-1B cell line. The status of hypermethylation was decreased after treatment with 5-Aza-CdR of 0.05, 0.1, 1, 5 nmol/ml, meanwhile, both methylation bands and demethylation bands were observed by methylation specific PCR. After the treatment with 5-Aza-CdR of 10 nmol/ml the hypermethylation was absent absolutely.
  
  
  CONCLUSIONS
  (1) In HEC-1B cell line, 5-Aza-CdR can inhibit cell proliferation and induce cell apopotosis. (2) 5-Aza-CdR can renew the expression of RASSF1A mRNA of HEC-1B cell line and reverse the hypermethylation of RASSF1A promoter.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226000, China. Email: jsnt_zhangyuquan@tom.com.
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Mao
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Ma
authors.forename: Mu
authors.initials: M
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Sheng
mesh_terms: D045744:Cell Line, Tumor; D019175:DNA Methylation; D016889:Endometrial Neoplasms / Q000235:genetics; D006801:Humans; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger