title: Identification of two critically deleted regions within chromosome segment 7q35-q36 in EVI1 deregulated myeloid leukemia cell lines.
abstract: Chromosomal rearrangements involving the EVI1 proto-oncogene are a recurrent finding in myeloid leukemias and are indicative of a poor prognosis. Rearrangements of the EVI1 locus are often associated with monosomy 7 or cytogenetic detectable deletions of part of 7q. As EVI1 overexpression alone is not sufficient to induce leukemia, loss of a 7q tumour suppressor gene might be a required cooperating event. To test this hypothesis, we performed high-resolution array comparative genomic hybridization analysis of twelve EVI1 overexpressing patients and three EVI1 deregulated cell lines to search for 7q submicroscopic deletions. This analysis lead to the delineation of two critical regions, one of 0.39 Mb on 7q35 containing the CNTNAP2 gene and one of 1.33 Mb on chromosome bands 7q35-q36 comprising nine genes in EVI1 deregulated cell lines. These findings open the way to further studies aimed at identifying the culprit EVI1 implicated tumour suppressor genes on 7q.
authors.affiliation: Centre for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: De Weer
authors.forename: Bruce
authors.initials: B
authors.lastname: Poppe
authors.forename: Sarah
authors.initials: S
authors.lastname: Vergult
authors.forename: Pieter
authors.initials: P
authors.lastname: Van Vlierberghe
authors.forename: Marjan
authors.initials: M
authors.lastname: Petrick
authors.forename: Robrecht
authors.initials: R
authors.lastname: De Bock
authors.forename: Yves
authors.initials: Y
authors.lastname: Benoit
authors.forename: Lucien
authors.initials: L
authors.lastname: Noens
authors.forename: Anne
authors.initials: A
authors.lastname: De Paepe
authors.forename: Nadine
authors.initials: N
authors.lastname: Van Roy
authors.forename: Björn
authors.initials: B
authors.lastname: Menten
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002897:Chromosomes, Human, Pair 7; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D000074008:MDS1 and EVI1 Complex Locus Protein; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics*; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000074008:MDS1 and EVI1 Complex Locus Protein; C063575:MECOM protein, human; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors