title: Integrative analysis of the heat shock response in Aspergillus fumigatus.
abstract: BACKGROUND
  Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30 degrees C to 48 degrees C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding proteins were differentially regulated upon heat shock.
  
  
  RESULTS
  91 differentially regulated protein spots, representing 64 different proteins, were identified by mass spectrometry (MS). They showed a continuous up-, down- or an oscillating regulation. Many of the identified proteins were involved in protein folding (chaperones), oxidative stress response, signal transduction, transcription, translation, carbohydrate and nitrogen metabolism. A correlation between alteration of transcript levels and corresponding proteins was detected for half of the differentially regulated proteins. Interestingly, some previously undescribed putative targets for the heat shock regulator Hsf1 were identified. This provides evidence for Hsf1-dependent regulation of mannitol biosynthesis, translation, cytoskeletal dynamics and cell division in A. fumigatus. Furthermore, computational analysis of promoters revealed putative binding sites for an AP-2alpha-like transcription factor upstream of some heat shock induced genes. Until now, this factor has only been found in vertebrates.
  
  
  CONCLUSIONS
  Our newly established DIGE data analysis workflow yields improved data quality and is widely applicable for other DIGE datasets. Our findings suggest that the heat shock response in A. fumigatus differs from already well-studied yeasts and other filamentous fungi.
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Albrecht
authors.forename: Reinhard
authors.initials: R
authors.lastname: Guthke
authors.forename: Axel A
authors.initials: AA
authors.lastname: Brakhage
authors.forename: Olaf
authors.initials: O
authors.lastname: Kniemeyer
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response; D006358:Hot Temperature; D020543:Proteome / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D020543:Proteome; D014157:Transcription Factors