title: Sensitive and rapid high-performance liquid chromatography tandem mass spectrometry method for estimation of fulvestrant in rabbit plasma.
abstract: A rapid and sensitive high-performance liquid chromatography and electrospray tandem mass spectrometry method was developed and validated for estimation of fulvestrant in rabbit plasma using liquid-liquid extraction. The separation and quantification of fulvestrant were achieved by reverse-phase chromatography on a Sunfire C18 column (50 x 2.1. i.d., 3.5 microm) with isocratic elution at a flow rate of 300 microL/min using norethistrone as an internal standard from 500 microL plasma sample. The method was validated over the concentration range from 0.092 to 16.937 ng/mL with a lower limit of detection of 0.023 ng/mL. The intra-day and inter-day accuracy and precision were within 10%. The recovery was 85 and 90% for fulvestrant and norethistrone respectively. The chromatographic run time was only 2.5 min.
authors.affiliation: Department of Pharmaceutical Analysis, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad-34, India.
authors.forename: Varanasi Murali
authors.initials: VM
authors.lastname: Balaram
authors.forename: Dharmesh
authors.initials: D
authors.lastname: Parmar
authors.forename: Bulusu B
authors.initials: BB
authors.lastname: Teja
authors.forename: Shivprakash
authors.initials: S
authors.lastname: Rathnam
authors.forename: Jangala Venkateswara
authors.initials: JV
authors.lastname: Rao
authors.forename: Bhavesh
authors.initials: B
authors.lastname: Dasandi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D000077267:Fulvestrant; D011817:Rabbits; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000077267:Fulvestrant; D004958:Estradiol