title: Engineered biological nanofactories trigger quorum sensing response in targeted bacteria.
abstract: Biological nanofactories, which are engineered to contain modules that can target, sense and synthesize molecules, can trigger communication between different bacterial populations. These communications influence biofilm formation, virulence, bioluminescence and many other bacterial functions in a process called quorum sensing. Here, we show the assembly of a nanofactory that can trigger a bacterial quorum sensing response in the absence of native quorum molecules. The nanofactory comprises an antibody (for targeting) and a fusion protein that produces quorum molecules when bound to the targeted bacterium. Our nanofactory selectively targets the appropriate bacteria and triggers a quorum sensing response when added to two populations of bacteria. The nanofactories also trigger communication between two bacterial populations that are otherwise non-communicating. We envision the use of these nanofactories in generating new antimicrobial treatments that target the communication networks of bacteria rather than their viability.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Rohan
authors.initials: R
authors.lastname: Fernandes
authors.forename: Varnika
authors.initials: V
authors.lastname: Roy
authors.forename: Hsuan-Chen
authors.initials: HC
authors.lastname: Wu
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
mesh_terms: D000907:Antibodies, Bacterial / Q000378:metabolism / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D005434:Flow Cytometry; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism / Q000494:pharmacology; D007783:Lactones / Q000378:metabolism / Q000494:pharmacology*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D053038:Quorum Sensing / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology; D012475:Salmonella / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D011993:Recombinant Fusion Proteins; D006714:Homoserine