title: Suppression of T cell proliferation by root apical papilla stem cells in vitro.
abstract: The use of allogeneic stem cells strongly extends the range of stem cell applications in dentistry; however, immunological rejection remains a major concern. There is little information about the immunological features of dental-related stem cells in the literature. Therefore, we investigated the immunological characteristics of stem cells from the root apical papilla (SCAP) of swine in vitro by measuring T cell immunomodulation and apoptosis. We found that SCAP expressed a low level of swine leukocyte antigen (SLA) class I molecules and were negative for SLA class II DR molecules. Moreover, SCAP could inhibit autologous T cell proliferation stimulated by phytohemagglutinin (PHA) and a one-way mixed lymphocyte reaction in a dose-dependent manner. In addition, SCAP could suppress proliferation of allogeneic T cells in a dose-dependent manner, with or without mitomycin C pretreatment. Moreover, soluble factor(s) may be involved in the SCAP-mediated immune suppression. After a 5-day coculture of SCAP, allogeneic T cells, and PHA, only 1.22% of T cells were apoptotic. These data indicated that SCAP were weakly immunogenic and suppressed T cell proliferation in vitro through an apoptosis-independent mechanism.
authors.affiliation: Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yunqing
authors.initials: Y
authors.lastname: An
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Songtao
authors.initials: S
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D013234:Stem Cells / Q000166:cytology / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D013601:T-Lymphocytes / Q000502:physiology*; D019227:Tooth Apex / Q000166:cytology* / Q000276:immunology*
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen