title: Antibody microarray typing, a novel technique for Streptococcus pneumoniae serotyping.
abstract: The use of antibody-based miniaturized devices for microbiological applications is a field poorly investigated in the era of more developed molecular amplification techniques. A novel antibody microarray for Streptococcus pneumoniae serotyping was developed, by printing nanolitre volumes of pneumococcal serotype-specific antibodies on multi-well slides. This microarray, which showed high specificity when tested against reference and clinical S. pneumoniae isolates, can be applicable as a faster, cost-effective and accurate serotyping technique for pneumococcal epidemiological studies.
authors.affiliation: Servicio de Microbiología, Hospital Donostia, San Sebastián, Spain. JOSEMARIA.MARIMONORTIZDEZ@osakidetza.net
authors.forename: J M
authors.initials: JM
authors.lastname: Marimon
authors.forename: A
authors.initials: A
authors.lastname: Monasterio
authors.forename: M
authors.initials: M
authors.lastname: Ercibengoa
authors.forename: J
authors.initials: J
authors.lastname: Pascual
authors.forename: I
authors.initials: I
authors.lastname: Prieto
authors.forename: L
authors.initials: L
authors.lastname: Simón
authors.forename: E
authors.initials: E
authors.lastname: Perez-Trallero
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D002648:Child; D006801:Humans; D011008:Pneumococcal Infections / Q000382:microbiology*; D040081:Protein Array Analysis / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000191:economics / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial