Measurement of long-range interatomic distances by solid-state tritium-NMR spectroscopy.
For the structural determination of a ligand bound to an amorphous macromolecular system, solid-state NMR can be used to provide interatomic distances. It is shown here that selective labeling in discrete locations with tritium enables accurate measurement of long-range distances owing to the high gyromagnetic ratio of this nucleus, without structural modification of the molecule. This approach gives access to the largest NMR distance ever measured between two nuclei (14.4 A). (3)H MAS NMR appears to be a promising tool for structural applications in the biological and material sciences.
Yuen
Alexander K L
AK
CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, F-91191 Gif sur Yvette, France.
Lafon
Olivier
O
Charpentier
Thibault
T
Roy
Myriam
M
Brunet
Francine
F
Berthault
Patrick
P
Sakellariou
Dimitrios
D
Robert
Bruno
B
Rimsky
Sylvie
S
Pillon
Florence
F
Cintrat
Jean-Christophe
JC
Rousseau
Bernard
B
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D014316:Tritium
D014316:Tritium