Proteomic analysis of porcine saliva.
Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS-PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.
Gutiérrez
A M
AM
Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain.
Miller
I
I
Hummel
K
K
Nöbauer
K
K
Martínez-Subiela
S
S
Razzazi-Fazeli
E
E
Gemeiner
M
M
Cerón
J J
JJ
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008297:Male; D013058:Mass Spectrometry / Q000662:veterinary; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis
D015415:Biomarkers; D020543:Proteome; D012471:Salivary Proteins and Peptides