title: Crucial roles of membrane stability and its related proteins in the tolerance of peach fruit to chilling injury.
abstract: Proteome patterns in peach fruit (Prunus persica L.) stored at different low temperatures were examined in order to gain a better understanding why peach fruit is less prone to chilling injury when stored at 0 degrees C than at 5 degrees C. Some differently expressed proteins in peach fruit stored at 0 and 5 degrees C were identified using electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Among these proteins, four membrane stability related proteins, i.e., enolase, temperature-induced lipocalin, major allergen Pru p 1, and type II SK2 dehydrin were enhanced, but three proteins related to phenolic compounds metabolization, cinnamyl-alcohol dehydrogenase 5, cinnamyl-alcohol dehydrogenase 1, and chorismate mutase, were repressed in peach fruit at 0 degrees C as compared to that at 5 degrees C. The abundance of glucose-6-phosphate dehydrogenase, NADP-dependent isocitrate dehydrogenase, and NADP-dependent malic enzyme, which catalyze the reactions during sugar metabolism and energy pathways, was found to decrease in peach fruit stored at 0 degrees C. In addition, our data revealed that low temperature of 0 degrees C might regulate the endogenous H(2)O(2) level, resulting in activating the transcriptional level of genes encoding the proteins related to membrane stability. These results provide a comprehensive knowledge to understand the mechanisms by which peach fruit stored at 0 degrees C showed a higher chilling tolerance than that at 5 degrees C.
authors.affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Handian District, 100093, Beijing, China.
authors.forename: Changfeng
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Ding
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Guozheng
authors.initials: G
authors.lastname: Qin
authors.forename: Shiping
authors.initials: S
authors.lastname: Tian
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D005519:Food Preservation; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D027861:Prunus / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D010940:Plant Proteins