Exacerbation of corneal scarring in HSV-1 gK-immunized mice correlates with elevation of CD8+CD25+ T cells in corneas of ocularly infected mice.
We have shown previously that exacerbation of corneal scarring (CS) in HSV-1 glycoprotein K (gK) immunized mice was associated with CD8+ T cells. In this study, we investigated the type and the nature of the immune responses that are involved in the exacerbation of CS in gK-immunized animals. BALB/c mice were vaccinated with baculovirus expressed gK, gD, or mock-immunized. Twenty-one days after the third immunization, mice were ocularly infected with 2 x 10(5) PFU/eye of virulent HSV-1 strain McKrae. Infiltration of the cornea by CD4+, CD8+, CD25+, CD4+CD25+, CD8+CD25+, CD19+, CD40+, CD40L+, CD62L+, CD95+, B7-1+, B7-2+, MHC-I+, and MHC-II+ cells was monitored by immunohistochemistry, qRT-PCR and FACS at various times post-infection (PI). This study demonstrated for the first time that the presence of CD8+CD25+ T cells in the cornea is correlated with exacerbation of CS in the gK-immunized group.
Allen
Sariah J
SJ
Center for Neurobiology and Vaccine Development, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA; Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
Mott
Kevin R
KR
Center for Neurobiology and Vaccine Development, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA; Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
Ljubimov
Alexander V
AV
Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA.
Ghiasi
Homayon
H
Center for Neurobiology and Vaccine Development, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA; Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA. Electronic address: ghiasih@CSHS.org.
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology*; D002921:Cicatrix / Q000276:immunology / Q000821:virology*; D003315:Cornea / Q000276:immunology / Q000821:virology*; D005260:Female; D005434:Flow Cytometry; D018259:Herpesvirus 1, Human; D007114:Immunization; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D016849:Keratitis, Herpetic / Q000276:immunology / Q000821:virology*; D017254:Leukemic Infiltration / Q000276:immunology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D013950:Thymus Gland / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D053645:Interleukin-2 Receptor alpha Subunit; C072448:UL53 protein, Human herpesvirus 1; D014764:Viral Proteins