Direct determination of tissue aminothiol, disulfide, and thioether levels using HPLC-ECD with a novel stable boron-doped diamond working electrode.
This chapter describes two different methods using reversed-phase HPLC with electrochemical detection on a boron-doped diamond (BDD) working electrode for the direct, routine, sensitive and simultaneous measurement of a number of aminothiols, disulfides, and thioethers, in either plasma or tissue homogenates.
Bailey
Bruce
B
ESA Biosciences, Inc, Chelmsford, MA, USA. bbailey@esainc.com
Waraska
John
J
Acworth
Ian
I
D000818:Animals; D001895:Boron / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000032:analysis; D018130:Diamond; D004220:Disulfides / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005978:Glutathione / Q000032:analysis; D006710:Homocysteine / Q000032:analysis; D006801:Humans; D015203:Reproducibility of Results; D013440:Sulfides / Q000032:analysis*
D004220:Disulfides; D013440:Sulfides; D006710:Homocysteine; D018130:Diamond; D005978:Glutathione; D003545:Cysteine; D001895:Boron