16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures.
A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.
Justesen
Ulrik Stenz
US
Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark. ujustesen@health.sdu.dk
Skov
Marianne Nielsine
MN
Knudsen
Elisa
E
Holt
Hanne Marie
HM
Søgaard
Per
P
Justesen
Tage
T
D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S