[Application of SPSS orthogonal design in tissue culture of Anoectochilus roxburghii].
OBJECTIVE
To study the effect of the different constitutions of plant hormone on the development of Anoectochilus roxburghii.


METHODS
A. roxburghii were harvested after having been cultured for 60 days. An orthogonal design was used to study the effect of NAA and 6-BA on the leaf number, eustipe number, lateral branch number of the stem tip and stem section, and the height of the stem tips. All of the data were processed by SPSS.


CONCLUSIONS
It is reported for the first time that NAA could make different development of A. roxburghii at low concentration ( < 1 mg L(-1)) and high concentration ( > 1 mg L(-1)). The optimum constitution of MS medium was NAA 0.5 mg L(-1) + 6-BA 1 mg L(-1) for the growth of the stem tip of A. roxburghii, and NAA 1 mg L(-1) + 6-BA 2 mg L(-1) for the differentiation of bud and the formation of lateral branch of the stem section. The different concentrations of NAA and 6-BA had different effects on the growth and differentiation of the stem tip and the stem section of A. roxburghii.
Zhang
Fusheng
F
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Guo
Shunxing
S
D001593:Benzyl Compounds; D003627:Data Interpretation, Statistical; D007701:Kinetin / Q000378:metabolism; D009280:Naphthaleneacetic Acids / Q000378:metabolism; D029595:Orchidaceae / Q000254:growth & development* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D011687:Purines; D012984:Software; D046509:Tissue Culture Techniques
D001593:Benzyl Compounds; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C480551:benzylaminopurine; D007701:Kinetin