title: In silico analysis of Ardmore, a novel mycobacteriophage isolated from soil.
abstract: Ardmore is a novel mycobacteriophage isolated from a soil sample collected in County Waterford, Ireland. The genome of this phage has been fully sequenced and is composed of 52,141 bp of linear double stranded DNA with a GC content of 61.49%. 88 ORFs were identified of which 34 were assigned predicted functions based on their homology to previously characterised proteins, their location in the genome, computer-predicted structural characteristics or presence of conserved motifs in their sequence. The Ardmore genome appears highly similar to mycobacteriophages Fruitloop and Tweety with BLASTn analysis showing 87% and 80% identity respectively. A predicted beta-lactamase gene was detected in the sequence, and an unusual +1 frameshift event for the translation of tail genes was also observed.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Ireland.
authors.forename: Marine
authors.initials: M
authors.lastname: Henry
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.forename: Roy D
authors.initials: RD
authors.lastname: Sleator
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Olivia
authors.initials: O
authors.lastname: McAuliffe
authors.forename: Jim M
authors.initials: JM
authors.lastname: O'Mahony
mesh_terms: D019295:Computational Biology; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D009160:Mycobacteriophages / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D012988:Soil Microbiology
chemical_list: D004279:DNA, Viral