title: Cloning and characterization of the zeaxanthin glucosyltransferase gene (crtX) from the astaxanthin-producing marine bacterium, Paracoccus haeundaensis.
abstract: Zeaxanthin glucosyltransferase (CrtX) mediates the formation of zeaxanthin to zeaxanthin diglucoside. Here, we report cloning of the crtX gene responsible for zeaxanthin diglucoside biosynthesis from Paracoccus haeundaensis and the production of the corresponding carotenoids in transformed cells carrying this gene. An expression plasmid containing the crtX gene (pSTCRT-X) was constructed, and Escherichia coli cells containing this plasmid produced the recombinant protein of approximately 46 kDa. Biosynthesis of zeaxanthin diglucoside was obtained when the plasmid pSTCRT-X was co-transformed into E. coli containing the pET-44a(+)-CrtEBIYZ carrying crtE, crtB, crtI, crtY, and crtZ genes required for zeaxanthin beta-D-diglucoside biosynthesis.
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 608-737, Korea.
authors.forename: Yong Bae
authors.initials: YB
authors.lastname: Seo
authors.forename: Seong Seok
authors.initials: SS
authors.lastname: Choi
authors.forename: Soo Wan
authors.initials: SW
authors.lastname: Nam
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
mesh_terms: D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial; D005960:Glucosides / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology; D008970:Molecular Weight; D010230:Paracoccus / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014871:Water Microbiology; D024341:Xanthophylls / Q000096:biosynthesis; D065146:Zeaxanthins
chemical_list: D005960:Glucosides; D011994:Recombinant Proteins; D024341:Xanthophylls; D065146:Zeaxanthins; C549597:zeaxanthin diglucoside; C005948:astaxanthine; D005964:Glucosyltransferases; C077175:zeaxanthin glucosyltransferase