title: tRNA mitochondrial import in yeast: Mapping of the import determinants in the carrier protein, the precursor of mitochondrial lysyl-tRNA synthetase.
abstract: Mitochondria of many species import of nuclear DNA-encoded tRNAs. This widely spread but poorly studied phenomenon proved to be a promising tool for mitochondrial transfection. In yeast Saccharomyces cerevisiae, one cytosolic tRNAs(Lys) is partially targeted into mitochondria. Previous studies have shown that binding of this tRNA to its putative protein carrier, the precursor of mitochondrial lysyl-tRNA synthetase (preMsk1p), IIb class aminoacyl-tRNA synthetase, was a pre-requisite of import. In this work, we identify the hinge region with two adjacent helices H5 and H7 to be responsible for mitochondrial targeting of the tRNA and characterize preMsk1p versions with altered tRK1 import capacities.
authors.affiliation: UMR No. 7156, CNRS - Université de Strasbourg, Department Molecular and Cellular Genetics, 21 rue René Descartes, 67084 Strasbourg, France.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kamenski
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Smirnova
authors.forename: Olga
authors.initials: O
authors.lastname: Kolesnikova
authors.forename: Igor A
authors.initials: IA
authors.lastname: Krasheninnikov
authors.forename: Robert P
authors.initials: RP
authors.lastname: Martin
authors.forename: Nina
authors.initials: N
authors.lastname: Entelis
authors.forename: Ivan
authors.initials: I
authors.lastname: Tarassov
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012357:RNA, Transfer, Lys / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017384:Sequence Deletion
chemical_list: D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D050505:Mutant Proteins; D011498:Protein Precursors; D012357:RNA, Transfer, Lys; D029701:Saccharomyces cerevisiae Proteins; D008250:Lysine-tRNA Ligase