Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori.
In this study, we have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as a mRNA cap-binding protein. The second gene is BmFOXG1, belonging to a family of forkhead transcription factors, FOXG1. The third gene is MBF2-related (MBF2-R) whose product has high homology to a co-activator protein MBF2 from B. mori. Although BmFOXG1 was up-regulated in the fat body in response to three kinds of bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, eIF4E-1 and MBF2-R were up-regulated by E. coli and B. subtilis, but not S. aureus, suggesting that bacteria possessing meso-diaminopimelic acid-containing peptidoglycan but not lysine-containing peptidoglycan activate eIF4E-1 and MBF2-R, probably through a conserved immune deficiency pathway. We further profiled the expression of three genes in different tissues and a silkworm cell line, NIAS-Bm-aff3, in response to bacteria, and at different times after bacterial challenge in the fat body.
Tanaka
Hiromitsu
H
Innate Immunity Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan. htanaka1@nias.affrc.go.jp
Suzuki
Noriko
N
Nakajima
Yoshiro
Y
Sato
Masaaki
M
Sagisaka
Aki
A
Fujita
Kosuke
K
Ishibashi
Jun
J
Imanishi
Shigeo
S
Mita
Kazuei
K
Yamakawa
Minoru
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D002460:Cell Line; D004926:Escherichia coli / Q000502:physiology; D005216:Fat Body / Q000378:metabolism; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000235:genetics*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D019476:Insect Proteins