The putative-farnesoic acid O-methyl transferase (FAMeT) gene of Ceratitis capitata: characterization and pre-imaginal life expression.
Farnesoic acid O-methyl transferase (FAMeT) is the enzyme involved in the penultimate step of insect juvenile hormone (JH) biosynthesis and is thus a key regulator in insect development and reproduction. We report the characterization of the putative-FAMeT in the medfly or Mediterranean fruit fly, Ceratitis capitata. This gene was identified by suppressive subtractive hybridization and completely sequenced by the screening of a medfly cDNA library. The obtained sequence was analyzed for conserved protein domain identification and its expression profile was evaluated by quantitative Real-Time PCR in medfly pre-imaginal life. The tissue expression of the isolated gene was verified by in situ hybridization on third instar larvae sections. The characterization of the isolated gene pointed out several typical features of methyl transferase genes. The pre-imaginal putative-FAMeT expression levels were consistent with JH titer change in Diptera. As recognized in some crustaceans, this gene seems to be widely expressed in the medfly as well. Ceratitis capitata is one of the most relevant agricultural pests against which insecticides and the sterile insect technique (SIT) are extensively used in spite of the well-known limitations of these approaches. Although results are not conclusive for the physiological role of the isolated gene, they suggest the characterization of a new gene in the Mediterranean fruit fly potentially involved in JH biosynthesis and may, therefore, have implications for pest control.
Vannini
Laura
L
Department of Evolutionary Biology, University of Siena, Italy. vannini18@unisi.it
Ciolfi
Silvia
S
Spinsanti
Giacomo
G
Panti
Cristina
C
Frati
Francesco
F
Dallai
Romano
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D033622:Ceratitis capitata / Q000201:enzymology / Q000235:genetics*; D020869:Gene Expression Profiling; D007605:Juvenile Hormones / Q000096:biosynthesis; D007814:Larva / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D007605:Juvenile Hormones; D008780:Methyltransferases; C032433:farnesoic acid O-methyltransferase