[Neuroprotective effects and mechanisms of Chuanxiong Chatiao pulvis against MPTP-induced dopaminergic neurotoxicity in mice model of Parkinson's disease].
OBJECTIVE
To investigate the neuroprotective effects and mechanisms of Chunxiong Chatiao pulvis (CXCT) on the MPTP-induced dopaminergic neurodegneration in mice model of PD.


METHODS
Three to five months old male mice were randomly divided into following six treatment groups (NS + NS, 50 mg x kg(-1) CXCT + NS, 25 mg x kg(-1) CXCT + MPTP, 50 mg x kg(-1) CXCT + MPTP, 75 mg x kg(-1) CXCT + MPTP, NS + MPTP) (n = 8/group). For behavioral measurement, the mice were evaluated for their motor coordination by pole-test; Striatal dopamine content of mice was measured using standard reverse-phase HPLC; Number of tyrosine hydroxylase (TH)-positive cells in the substantia nigra of mice was detected by immunohistochemistry; SOD activity in the substantia nigra of mice were measured by fluorimetry.


RESULTS
The score of pole test (25, 50, 75 mg x kg(-1)), the striatal dopamine contents (75 mg x kg-1), the residual DA contents (75 mg x kg(-1)) and the SOD activity in substantia nigra (50, 75 mg x kg(-1)) all showed that, the CXCT-pretreated groups significantly higher than that of the group NS + MPTP (P < 0.05 or P < 0.01).


CONCLUSIONS
Chuanxiong Chatiao pulvis improved the motor deficit in MPTP-induced mice, and attenuate MPTP-induced dopaminergic neurodegeneration in mice. Importantly, the neuroprotective effect of Chuanxiong Chatiao pulvis against dopaminergic neurodegeneration may be associated with its strong antioxidant capacity in vivo.
Shu
Dan
D
Experimental Neurobiology Research Institute of Wenzhou Medical College, Wenzhou 325000, China.
He
Jincai
J
Chen
Jiangfan
J
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000009:adverse effects*; D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010300:Parkinson Disease / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D004298:Dopamine