title: Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea.
abstract: The first Korean strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in 1997, and it exhibited high similarity to strain VR-2332 (type II PRRSV; North American type). Recently, however, infection with type I PRRSV (European type) has also been reported in Korea. To date, preliminary data about type I PRRSV prevalence in Korea have not been reported. Here, using reverse transcriptase (RT)-PCR, we analyzed 383 archived field samples from 101 pig farms in Korea that were collected from 2007 to 2008. We identified 155 samples from 68 farms that were positive for PRRSV. Fifty-one samples (51/155; 32.9%) and 20 farms (20/68; 29.4%) were type I PRRSV-positive/type II PRRSV-negative. Furthermore, we tried to isolate the type I PRRSV from positive samples and seven type I PRRSV were isolated using PAM. The phylogenetic analysis using the type I PRRSV isolates (7 isolates) was performed based on open reading frame (ORF)5 (accession numbers GU325642 to GU325648) and ORF7 (accession numbers GU325635 to GU325641). In the phylogenetic study, seven type I PRRSV isolates were closely related with panEuropean based on ORF7, while they were genetically distinct from Lelystad virus and made a unique clade based on ORF5. The results of this study demonstrate that infection with type I PRRSV is not uncommon in Korean pig farms, which suggests that diagnosis and control of type I PRRSV should be considered in Korea. A new approach to vaccination against, and epidemiological analysis of, Korean PRRSV is urgently needed.
authors.affiliation: Chung-Aang University School of Medicine, Seoul 156-756, Republic of Korea.
authors.forename: Chulseung
authors.initials: C
authors.lastname: Lee
authors.forename: Hyekwon
authors.initials: H
authors.lastname: Kim
authors.forename: Bokyu
authors.initials: B
authors.lastname: Kang
authors.forename: Minjoo
authors.initials: M
authors.lastname: Yeom
authors.forename: Sangyoon
authors.initials: S
authors.lastname: Han
authors.forename: Hyoungjoon
authors.initials: H
authors.lastname: Moon
authors.forename: Seongjun
authors.initials: S
authors.lastname: Park
authors.forename: Hyunil
authors.initials: H
authors.lastname: Kim
authors.forename: Daesub
authors.initials: D
authors.lastname: Song
authors.forename: Bongkyun
authors.initials: B
authors.lastname: Park
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D012367:RNA, Viral