[Infiltration of CD(4)(+)CD(25)(+) T cells into the allergic asthmatic airways caused by house dust mite antigen administration.].
OBJECTIVE
To investigate whether house dust mite (HDM) could induce CD(4)(+) CD(25)(+) T cells infiltration into asthmatic airways in patients vivo.


METHODS
Ten subjects with asthma underwent initial bronchoscopy during which normal saline and HDM were administered to two sublobar segments separately. The second bronchoscopy were carried out and bronchoal lavage fluid from HDM-challenged sites and saline-challenged sites were separately taken 24 h later. The differential cell counts were determined, and the absolute number of each type was calculated. At the same time, CD(3)(+), CD(4)(+) and CD(4)(+) CD(25)(+) T cells were determined by flow-cytometric analysis. We compared cellular counts in airways without and after topical instillation of HDM.


RESULTS
Eosinophile granulocyte cells of broncho-alveolar fluid in the HDM-challenged sites (1.4 +/- 0.1) x 10(6)/ml are more than it in control sites (0.3 +/- 0.1) x 10(6)/ml, P < 0.003. Lymphocyte cells of BALF in the HDM-challenged sites (2.2 +/- 0.3) x 10(6)/ml are more than it in control sites (0.3 +/- 0.1) x 10(6)/ml, P < 0.001; CD(4)(+) CD(25)(+)T cells of BALF in the HDM-challenged sites (784.0 +/- 281.3) cell/microl are more than it in control sites (7.7 +/- 3.6) cell/microl, P < 0.001.


CONCLUSIONS
Our findings suggest that HDM is capable of inducing CD(4)(+) CD(25)(+) T cells recruitment into non-acute mild allergic asthmatic airways.
Chen
Yi-Qiang
YQ
Institute of Respiratory Medicine of Guangxi Medical University, Nanning, Guangxi 530021, China. Email: chenyq163@tom.com.
Huang
Hong-Dong
HD
Wen
Hong-Xia
HX
Zou
Xiao-Ying
XY
Deng
Jing-Min
JM
Shi
Huan-Zhong
HZ
D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma; D001992:Bronchoalveolar Lavage Fluid; D004804:Eosinophils; D006801:Humans; D039981:Pyroglyphidae; D013601:T-Lymphocytes
D039741:Antigens, Dermatophagoides