Herpesvirus-associated papillomatosis in a green lizard.
Papillomatous skin lesions from a green lizard (Lacerta viridis) were examined histologically, using electron microscopy and DNA was isolated from the lesions for herpes-viral DNA detection. Histology confirmed the lesions to be squamous epithelial papillomas. Using electron microscopy, no virus particles were detected. The specific sequence of herpesviral DNA-directed DNA polymerase (EC 2.7.7.7) was amplified using degenerate primers in a nested format. The 235-base-pair (bp) sequence was sequenced and compared with previously published DNA-directed DNA polymerase sequences from various reptile herpesviruses. The sequence from the green lizard showed significant similarity with sequence of fibropapilloma-associated turtle herpesviruses from sea turtles.
Literak
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I
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic.
Robesova
B
B
Majlathova
V
V
Majlath
I
I
Kulich
P
P
Fabian
P
P
Roubalova
E
E
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D006564:Herpesviridae / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D006814:Hungary / Q000453:epidemiology; D008116:Lizards / Q000821:virology*; D008854:Microscopy, Electron / Q000662:veterinary; D010212:Papilloma / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D014426:Turtles / Q000821:virology
D004279:DNA, Viral