Isolation and identification of arabinose mycolates of Cell Wall Skeleton (CWS) derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105).
A unique hydrolysis method using a two-layer solution, consisting of diluted hydrochloric acid and toluene was developed to isolate whole arabinose mycolates from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) in order to reveal its pivotal role in enhancing immune responses against tumors.
Uenishi
Yuko
Y
Technology Research & Development Division, Dainippon Sumitomo Pharma Co., Ltd., Konohana-ku, Osaka 554-0022, Japan. yuko-uenishi@ds-pharma.co.jp
Kusunose
Naoto
N
Yano
Ikuya
I
Sunagawa
Makoto
M
D000276:Adjuvants, Immunologic / Q000737:chemistry; D001089:Arabinose / Q000031:analogs & derivatives* / Q000737:chemistry; D017358:Cell Wall Skeleton / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D015394:Molecular Structure; D009163:Mycobacterium bovis / Q000737:chemistry*; D009171:Mycolic Acids / Q000737:chemistry / Q000302:isolation & purification; D014041:Tokyo; D014050:Toluene / Q000737:chemistry
D000276:Adjuvants, Immunologic; D017358:Cell Wall Skeleton; D009171:Mycolic Acids; D014050:Toluene; D001089:Arabinose; D006851:Hydrochloric Acid