The role of reactive oxygen species in the induction of Ty1 retrotransposition in Saccharomyces cerevisiae.
Here we provide evidence for a dependence between the increased production of reactive oxygen species and the activation of Ty1 retrotransposition. We have found that the strong activator of Ty1 mobility, methylmethane sulphonate, can not induce Ty1 retrotransposition in cells with compromised mitochondrial oxidative phosphorylation (rho(-); sco1Delta), which is the major source for production of reactive oxygen species (ROS) in Saccharomyces cerevisiae. The quantitative estimation of superoxide anions in living cells showed that rho(+) cells exposed to methylmethane sulphonate increase Ty1 retrotransposition and superoxide levels. The increase of superoxide anions by the superoxide generator menadione is accompanied by induction of Ty1 mobility without any treatment with a DNA-damaging agent. Higher frequencies of retrotransposition were found in rho(+) and rho(-) cells treated with exogenously added hydrogen peroxide or in cells with disrupted YAP1 gene characterized by increased intracellular levels of hydrogen peroxide. These data indicate that increased levels of ROS may have an independent and key role in the induction of Ty1 retrotransposition.
Stoycheva
Teodora
T
Institute of Cryobiology and Food Technology, Department of Cryobiology and Lyophilization, 53A Cherni Vrah Blvd., 1407 Sofia, Bulgaria. teodora.stoycheva@ikht.bg
Pesheva
Margarita
M
Venkov
Pencho
P
D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018626:Retroelements; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D024483:Vitamin K 3 / Q000378:metabolism
D017382:Reactive Oxygen Species; D018626:Retroelements; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D013481:Superoxides; D024483:Vitamin K 3; D008741:Methyl Methanesulfonate; D006861:Hydrogen Peroxide