Interaction of hepatitis B virus core protein with human GIPC1.
Up to now, little is known about hepatitis B virus core protein (HBc) interactions with host-cell proteins, although such interactions might be essential for virus propagation and pathogenicity. In this work, a human liver cDNA library was screened for proteins interacting with HBc. Among several HBc-interacting partners selected, it interacted most strongly with the human protein GIPC1. A common protein interaction domain, PDZ, was identified as the region that is sufficient for the interaction with HBc. The core protein has a putative C-terminal PDZ-interacting motif, and this sequence proved to be important for the interaction with GIPC1.
Razanskas
Raimundas
R
Institute of Biotechnology, Graiciuno 8, 02241, Vilnius, Lithuania. raimis@ibt.lt
Sasnauskas
Kestutis
K
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D006512:Hepatitis B Core Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping
D048868:Adaptor Proteins, Signal Transducing; C492009:GIPC1 protein, human; D006512:Hepatitis B Core Antigens