Proposal of a punch biopsy protocol as a pre-requisite for the establishment of a tissue bank from resected esophageal tumors.
With the development of tissue banking, a need for homogeneous methods of collection, processing, and storage of tissue has emerged. We describe the implementation of a biological bank in a high-volume, tertiary care University referral center for esophageal cancer surgery. We also propose an original punch biopsy technique of the surgical specimen. The method proved to be simple, reproducible, and not expensive. Unified standards for specimen collection are necessary to improve results of specimen-based diagnostic testing and research in surgical oncology.
Bonavina
Luigi
L
Department of Medical and Surgical Sciences, Division of General Surgery, IRCCS Policlinico San Donato, University of Milano Medical School, Milan, Italy. luigi.bonavina@unimi.it
Laface
Letizia
L
Picozzi
Stefano
S
Nencioni
Marco
M
Siboni
Stefano
S
Bona
Davide
D
Sironi
Andrea
A
Sorba
Francesca
F
Clemente
Claudio
C
D001706:Biopsy / Q000379:methods*; D002277:Carcinoma / Q000473:pathology / Q000601:surgery*; D004938:Esophageal Neoplasms / Q000473:pathology / Q000601:surgery*; D006801:Humans; D008495:Medical Oncology; D010340:Pathology, Surgical; D013048:Specimen Handling / Q000379:methods* / Q000592:standards*; D014015:Tissue Banks / Q000592:standards