Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis.
In plants, the mechanisms that regulate the transit of vacuolar soluble proteins containing C-terminal and N-terminal vacuolar sorting determinants (VSDs) to the vacuole are largely unknown. In a screen for Arabidopsis thaliana mutants affected in the trafficking of C-terminal VSD containing proteins, we isolated the ribosomal biogenesis mutant rpl4a characterized by its partial secretion of vacuolar targeted proteins and a plethora of developmental phenotypes derived from its aberrant auxin responses. In this study, we show that ribosomal biogenesis can be directly regulated by auxins and that the exogenous application of auxins to wild-type plants results in vacuolar trafficking defects similar to those observed in rpl4a mutants. We propose that the influence of auxin on ribosomal biogenesis acts as a regulatory mechanism for auxin-mediated developmental processes, and we demonstrate the involvement of this regulatory mechanism in the sorting of vacuolar targeted proteins in Arabidopsis.
Rosado
Abel
A
Department of Botany and Plant Sciences and Center for Plant Cell Biology, University of California, Riverside, California 92521, USA.
Sohn
Eun Ju
EJ
Drakakaki
Georgia
G
Pan
Songqin
S
Swidergal
Alexandra
A
Xiong
Yuqing
Y
Kang
Byung-Ho
BH
Bressan
Ray A
RA
Raikhel
Natasha V
NV
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009154:Mutation; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D014617:Vacuoles / Q000378:metabolism*
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D020543:Proteome; D018749:RNA, Plant; D012269:Ribosomal Proteins; C036483:T-DNA; C056368:ribosomal protein L4