Expression of Runx2 and type X collagen in vertebral growth plate of patients with adolescent idiopathic scoliosis.
The different expression of type X collagen and Runx2 between the convex and concave side of vertebral growth plate in scoliosis may help to improve our understanding of the role that growth plate tissue play in the development or progression of idiopathic scoliosis. In this investigation, there were significant differences of the total expression of type X collagen, Runx2 protein, and Runx2 mRNA between convex side and concave side growth plates of the apex vertebrae (p < 0.05). The total expression of type X collagen in the concave side growth plates of the lower end vertebrae was higher than that in the same side growth plates of apex (p < 0.05). The total expression of Runx2 in the concave side growth plates in the upper and lower end vertebrae were higher than that in the concave side growth plates of apex (p < 0.05). The expression of type X collagen, Runx2, and Runx2 mRNA, the cell density of type X collagen and Runx2 positive chondrocytes, and histological changes between convex side and concave side of the vertebral growth plate indicated that the vertebral growth plate was affected by mechanical forces, which was a secondary change and could contribute to progression of adolescent idiopathic scoliosis.
Wang
Shoufeng
S
Spine Surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.
Qiu
Yong
Y
Ma
Zhaolong
Z
Xia
Caiwei
C
Zhu
Feng
F
Zhu
Zezhang
Z
D000293:Adolescent; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002648:Child; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D024121:Collagen Type X / Q000235:genetics / Q000378:metabolism*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006132:Growth Plate / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012600:Scoliosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013131:Spine / Q000378:metabolism* / Q000473:pathology
D024121:Collagen Type X; D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; C493709:RUNX2 protein, human