MC1R stimulation by alpha-MSH induces catalase and promotes its re-distribution to the cell periphery and dendrites.
We demonstrated a direct correlation between melanogenic and catalase activities on in vitro and ex vivo models. Here, we investigated whether the stimulation of Melanocortin-1 Receptor (MC1R) could influence catalase expression, activity and cellular localization. For this purpose, we treated B16-F0 melanoma cells with alpha-Melanocyte Stimulating Hormone (alpha-MSH) and we showed a rapid induction of catalase through a cAMP/PKA-dependent, microphthalmia-associated transcription factor (MITF) independent mechanism, acting at post-transcriptional level. Moreover, alpha-MSH promoted a partial re-distribution of catalase to the cell periphery and dendrites. This work strengthens the correlation between melanogenesis and anti-oxidants, demonstrating the induction of catalase in response to a melanogenic stimulation, such as alpha-MSH-dependent MC1R activation. Moreover, this study highlights catalase regulatory mechanisms poorly known, and attributes to alpha-MSH a protective role in defending melanocytes, and possibly keratinocytes, not only on the basis of its pigmentary action, but also for its capacity to stimulate a quick anti-oxidant defence.
Maresca
Vittoria
V
Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy.
Flori
Enrica
E
Bellei
Barbara
B
Aspite
Nicaela
N
Kovacs
Daniela
D
Picardo
Mauro
M
D000818:Animals; D002374:Catalase / Q000096:biosynthesis / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D054746:Cyclic AMP-Dependent Protein Kinase Type II / Q000378:metabolism; D003712:Dendrites / Q000187:drug effects* / Q000201:enzymology; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D021381:Protein Transport / Q000187:drug effects; D044102:Receptor, Melanocortin, Type 1 / Q000819:agonists / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D000521:alpha-MSH / Q000494:pharmacology*
D044102:Receptor, Melanocortin, Type 1; D000521:alpha-MSH; D000242:Cyclic AMP; D002374:Catalase; D054746:Cyclic AMP-Dependent Protein Kinase Type II