Use of protein AG in an enzyme-linked immunosorbent assay for serodiagnosis of Toxoplasma gondii infection in four species of animals.
An enzyme-linked immunosorbent assay (ELISA) employing protein AG (AG-ELISA) as a conjugate was developed to detect anti-Toxoplasma gondii antibodies (Ab) in experimentally infected pigs and naturally infected pigs, goats, dogs, and cats. The results indicate that AG-ELISA can be a useful method for serological diagnosis of T. gondii infection in these four species of animals.
Zhang
Donglin
D
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Wang
Zhengsong
Z
Fang
Rui
R
Nie
Hao
H
Feng
Huihui
H
Zhou
Yanqin
Y
Zhao
Junlong
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D002415:Cats; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D006041:Goats; D006801:Humans; D012698:Serologic Tests / Q000379:methods* / Q000662:veterinary; D013552:Swine; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan