title: Herpesvirus-associated papillomatosis in a green lizard.
abstract: Papillomatous skin lesions from a green lizard (Lacerta viridis) were examined histologically, using electron microscopy and DNA was isolated from the lesions for herpes-viral DNA detection. Histology confirmed the lesions to be squamous epithelial papillomas. Using electron microscopy, no virus particles were detected. The specific sequence of herpesviral DNA-directed DNA polymerase (EC 2.7.7.7) was amplified using degenerate primers in a nested format. The 235-base-pair (bp) sequence was sequenced and compared with previously published DNA-directed DNA polymerase sequences from various reptile herpesviruses. The sequence from the green lizard showed significant similarity with sequence of fibropapilloma-associated turtle herpesviruses from sea turtles.
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Literak
authors.forename: B
authors.initials: B
authors.lastname: Robesova
authors.forename: V
authors.initials: V
authors.lastname: Majlathova
authors.forename: I
authors.initials: I
authors.lastname: Majlath
authors.forename: P
authors.initials: P
authors.lastname: Kulich
authors.forename: P
authors.initials: P
authors.lastname: Fabian
authors.forename: E
authors.initials: E
authors.lastname: Roubalova
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D006564:Herpesviridae / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D006814:Hungary / Q000453:epidemiology; D008116:Lizards / Q000821:virology*; D008854:Microscopy, Electron / Q000662:veterinary; D010212:Papilloma / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D014426:Turtles / Q000821:virology
chemical_list: D004279:DNA, Viral