title: Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton.
abstract: Drosophila melanogaster Kelch (KEL) is the founding member of a diverse protein family defined by a repeated sequence motif known as the KEL repeat (KREP). Several KREP proteins, including Drosophila KEL, bind filamentous actin (F-actin) and contribute to its organization. Recently, a subset of KREP proteins has been shown to function as substrate adaptor proteins for cullin-RING (really interesting new gene) ubiquitin E3 ligases. In this study, we demonstrate that association of Drosophila KEL with Cullin-3, likely in a cullin-RING ligase, is essential for the growth of Drosophila female germline ring canals. These results suggest a role for protein ubiquitylation in the remodeling of a complex F-actin cytoskeletal structure.
authors.affiliation: Department of Genetics, Yale University, New Haven, CT 06520, USA.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Hudson
authors.forename: Lynn
authors.initials: L
authors.lastname: Cooley
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D010063:Ovum / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000199:Actins; C503647:Cul3 protein, Drosophila; D044842:Cullin Proteins; D029721:Drosophila Proteins; D008840:Microfilament Proteins; C080353:kel protein, Drosophila