title: FRT-seq: amplification-free, strand-specific transcriptome sequencing.
abstract: We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Lira
authors.initials: L
authors.lastname: Mamanova
authors.forename: Robert M
authors.initials: RM
authors.lastname: Andrews
authors.forename: Keith D
authors.initials: KD
authors.lastname: James
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Sheridan
authors.forename: Peter D
authors.initials: PD
authors.lastname: Ellis
authors.forename: Cordelia F
authors.initials: CF
authors.lastname: Langford
authors.forename: Tobias W B
authors.initials: TW
authors.lastname: Ost
authors.forename: John E
authors.initials: JE
authors.lastname: Collins
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Turner
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D014157:Transcription Factors