title: Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions: in vivo and in vitro studies.
abstract: Since omega-3 polyunsaturated fatty acids (n-3 PUFAs) can alter ventricular myocyte calcium handling, these fatty acids could adversely affect cardiac contractile function, particularly following myocardial infarction. Therefore, 4 wk after myocardial infarction, dogs were randomly assigned to either placebo (corn oil, 1 g/day, n = 16) or n-3 PUFAs supplement [docosahexaenoic acid (DHA) + eicosapentaenoic acid (EPA) ethyl esters; 1, 2, or 4 g/day; n = 7, 8, and 12, respectively] groups. In vivo, ventricular function was evaluated by echocardiography before and after 3 mo of treatment. At the end of the 3-mo period, hearts were removed and in vitro function was evaluated using right ventricular trabeculae and isolated left ventricular myocytes. The treatment elicited significant (P < 0.0001) dose-dependent increases (16.4-fold increase with 4 g/day) in left ventricular tissue and red blood cell n-3 PUFA levels (EPA + DHA, placebo, 0.42 +/- 0.04; 1 g/day, 3.02 +/- 0.23; 2 g/day, 3.63 +/- 0.17; and 4 g/day, 6.97 +/- 0.33%). Regardless of the dose, n-3 PUFA treatment did not alter ventricular function in the intact animal (e.g., 4 g/day, fractional shortening: pre, 42.9 +/- 1.6 vs. post, 40.1 +/- 1.7%; placebo: pre, 39.2 +/- 1.3 vs. post, 38.4 +/- 1.6%). The developed force per cross-sectional area, changes in length- and frequency-dependent behavior in contractile force, and the inotropic response to beta-adrenoceptor activation were also similar for trabeculae obtained from placebo- or n-3 PUFA-treated dogs. Finally, calcium currents and calcium transients were the same in myocytes from n-3 PUFA- and placebo-treated dogs. Thus dietary n-3 PUFAs did not adversely alter either in vitro or in vivo ventricular contractile function in dogs with healed infarctions.
authors.affiliation: Dept. of Physiology and Cell Biology, The Ohio State Univ., Columbus, 43210-1218, USA. billman.1@osu.edu
authors.forename: George E
authors.initials: GE
authors.lastname: Billman
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nishijima
authors.forename: Andriy E
authors.initials: AE
authors.lastname: Belevych
authors.forename: Dmitry
authors.initials: D
authors.lastname: Terentyev
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Kaylan M
authors.initials: KM
authors.lastname: Haizlip
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Monasky
authors.forename: Nitisha
authors.initials: N
authors.lastname: Hiranandani
authors.forename: William S
authors.initials: WS
authors.lastname: Harris
authors.forename: Sandor
authors.initials: S
authors.lastname: Gyorke
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Carnes
authors.forename: Paul M L
authors.initials: PM
authors.lastname: Janssen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018408:Patch-Clamp Techniques; D014463:Ultrasonography; D016277:Ventricular Function, Left / Q000187:drug effects* / Q000502:physiology*
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids; C035276:eicosapentaenoic acid ethyl ester; C067518:4,7,10,13,16,19-docosahexaenoic acid ethyl ester; D015118:Eicosapentaenoic Acid; D002118:Calcium