Hepatitis B virus core interacts with the host cell nucleolar protein, nucleophosmin 1.
Hepatitis B virus (HBV) genome replication requires the packaging of viral factors (pregenomic RNA and polymerase) as well as host factors, including heat shock proteins and protein kinase C. Previous reports have suggested that there are several unidentified host factors that affect this encapsidation step. In this study, we identified a new host factor, nucleophosmin (B23) that interacts with the HBV core protein 149 (Cpl49). We analyzed this factor using NHS-activated sepharose resin and MALDI-TOF MS. Using the BIAcore analysis system, we were also able to deduce that the B23.1 residues 259-294 were required for the interaction between Cpl49 and B23.1 in vitro.
Lee
Su Jin
SJ
School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.
Shim
Hee Youn
HY
Hsieh
Antony
A
Min
Ji Young
JY
Jung
Gu hung
Gh
D002460:Cell Line; D002846:Chromatography, Affinity; D006512:Hepatitis B Core Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D011485:Protein Binding; D025941:Protein Interaction Mapping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019065:Virus Assembly
D006512:Hepatitis B Core Antigens; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin