Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis.
Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.
Choi
Yoo Seong
YS
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
Johannes
Tyler W
TW
Simurdiak
Michael
M
Shao
Zengyi
Z
Lu
Haige
H
Zhao
Huimin
H
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002867:Chromones / Q000378:metabolism; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008957:Models, Genetic; D005810:Multigene Family; D011753:Pyrones / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002867:Chromones; D011753:Pyrones; C508401:spectinabilin; C012198:aureothin