Polyaniline/carbon nanotubes platform for sexually transmitted disease detection.
Polyaniline/carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5'-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 x 10(-6) M to 1 x 10(-17) M with detection limit of 1.2 x 10(-17) M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB).
Singh
Renu
R
Department of Science & Technology Centre on Biomolecular Electronics, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi-110012, India.
Dhand
Chetna
C
Sumana
Gajjala
G
Verma
Rachna
R
Sood
Seema
S
Gupta
Rajinder Kumar
RK
Malhotra
Bansi Dhar
BD
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009344:Neisseria gonorrhoeae / Q000737:chemistry* / Q000235:genetics; D012680:Sensitivity and Specificity; D012749:Sexually Transmitted Diseases / Q000175:diagnosis*; D017550:Spectroscopy, Fourier Transform Infrared
D000814:Aniline Compounds; D004269:DNA, Bacterial; D037742:Nanotubes, Carbon; C416807:polyaniline