The nitric oxide synthase 3 gene polymorphisms and their association with deep vein thrombosis in Asian Indian patients.
BACKGROUND
Endothelium derived nitric oxide is formed from l-arginine by endothelial nitric oxide synthase encoded by the nitric oxide synthase 3 (NOS3) gene. Nitric oxide possesses a variety of protective effects on endothelial cells and therefore NOS3 is a logical candidate gene to be investigated for the susceptibility of deep vein thrombosis (DVT).


METHODS
One hundred consecutive patients (M: F=56:44) with idiopathic deep vein thrombosis and an equal number of age and sex matched healthy controls were the study subjects. All study subjects were typed for five NOS3 polymorphisms (-786C/T, -922A/G, 894G/T, Intron 4 VNTR, and Intron 23 G10T).


RESULTS
Two polymorphisms (-922A/G and 894G/T) are showing their association with DVT. -922A/G shows both genotypic (P=0.0218; chi2=5.25; O.R=1.94) as well as allelic association (P=0.0014; chi2=10.19; O.R=2.0) while 894G/T shows only allelic (P=0.04; chi2=3.93; O.R=3.93) association with DVT.


CONCLUSIONS
Susceptibility to DVT in North Indian Asian patients may be associated with some variants of NOS3 gene.
Akhter
Mohd Suhail
MS
All India Institute of Medical Sciences, New Delhi, India.
Biswas
Arijit
A
Ranjan
Ravi
R
Sharma
Amit
A
Kumar
Sunil
S
Saxena
Renu
R
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D020246:Venous Thrombosis / Q000235:genetics*; D055815:Young Adult
C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III