title: Identification and assignment of three disulfide bonds in mammalian leukocyte cell-derived chemotaxin 2 by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Mammalian leukocyte cell-derived chemotaxin 2 (LECT2) contains six evolutionarily conserved cysteine residues. To date, however, the presence of disulfide linkages between these residues has not been determined. To search for disulfide bonds, the protein was proteolitically digested and the resulting peptides were analyzed by matrix-assisted laser desorption/ionization?time of flight mass spectrometry. The analysis showed that murine and human LECT2 have three intramolecular disulfide bonds (Cys25-Cys60; Cys36-Cys41; Cys99-Cys142) and no free cysteine residues.
authors.affiliation: Department of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon University, Tokyo, Japan. okumura@phys.chs.nihon-u.ac.jp
authors.forename: Akinori
authors.initials: A
authors.lastname: Okumura
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Tomoya
authors.initials: T
authors.lastname: Okabe
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Nakazato
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ohno
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamagoe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D003545:Cysteine / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004220:Disulfides; D036341:Intercellular Signaling Peptides and Proteins; C103468:LECT2 protein, human; C491213:Lect2 protein, mouse; D003545:Cysteine