Delta-catenin affects the localization and stability of p120-catenin by competitively interacting with E-cadherin.
E-cadherin is a member of the cadherin family of Ca(2+)-dependent cell-cell adhesion molecules. p120-Catenin and delta-catenin are known to bind to similar juxtamembrane regions of E-cadherin, and p120-catenin is known to stabilize E-cadherin. However, the function of competition between p120-catenin and delta-catenin for E-cadherin has not been fully explained. In this report, we show that cells overexpressing delta-catenin contain less p120-catenin than control cells at the cell-cell interface and that this causes the relocalization of p120-catenin from the plasma membrane to the cytosol. We show that successful binding by either one to E-cadherin adversely affects the stability of the other.
Yang
Ilhwan
I
The College of Pharmacy and Research Institute for Drug Development, Chonnam National University, Gwangju, 500-757, Korea.
Chang
Ockyoung
O
Lu
Qun
Q
Kim
Kwonseop
K
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015854:Up-Regulation; D000095002:Delta Catenin
D015820:Cadherins; D051177:Catenins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D000095002:Delta Catenin