title: On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine.
abstract: A new assay for urinary homocysteine is described. The assay relies on an on-column derivatization with o-phthaldialdehyde, using a reversed-phase HPLC column and detection/quantification by fluorescence. The analysis time for reduced and total homocysteine, including sample work-up, was 5 and 13 min, respectively. Quantification limit was 25 nM.
authors.affiliation: Department of Environmental Chemistry, University of Łódź, 90-131 Łódź, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Głowacki
authors.forename: Kamila
authors.initials: K
authors.lastname: Borowczyk
authors.forename: Edward
authors.initials: E
authors.lastname: Bald
authors.forename: Hieronim
authors.initials: H
authors.lastname: Jakubowski
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006710:Homocysteine / Q000652:urine*; D006801:Humans; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D006710:Homocysteine; D009764:o-Phthalaldehyde