title: Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors.
abstract: Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab(2) as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab(2) or MSN-HRP-Ab(2) as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H(2)O(2). The immunosensor using MSN-TH-Ab(2) as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules.
authors.affiliation: Department of Mechanical Engineering, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
authors.forename: Minghui
authors.initials: M
authors.lastname: Yang
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Alireza
authors.initials: A
authors.lastname: Javadi
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Gong
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007074:Immunoglobulin G; D012822:Silicon Dioxide