title: Multilocus sequence typing of sequential Candida albicans isolates from patients with persistent or recurrent fungemia.
abstract: Multilocus sequence typing (MLST) is a useful tool to explore the phylogenetics and epidemiology of Candida albicans isolates recovered from cases of invasive candidiasis. The goal of this study was to determine whether the same or different strains were responsible for persistent or recurrent fungemia through the use of MLST and ABC typing on sequential C. albicans isolates from the same patient. We applied both typing methods to 21 C. albicans strains recovered from 8 patients with persistent or recurrent candidemia. The isolates were collected during a multicenter surveillance study in four public tertiary care hospitals in Brazil. Persistent candidemia was defined as two or more blood cultures positive for C. albicans on 2 or more separate days. Recurrent candidemia was defined as an episode of candidemia occurring at least 1 month after the apparent complete resolution of an infectious episode caused by Candida species. We observed that, except for one patient, all strains from the first and second samples of the same patient showed the same MLST diploid sequence type (DST), ABC type and susceptibility profile to antifungals. Three distinct strains, well discriminated by MLST, were found in the seven samples collected sequentially over 10 days from one patient. The strains from the first four samples were indistinguishable, the fifth and sixth were also indistinguishable but different from the first four and seventh samples. Significantly, the seventh strain was the only C. albicans clade 2 isolate found in our total collection involving 61 patients, although clade 2 is commonly found worldwide. To the best of our knowledge, this is the first study describing the recovery of three distinct C. albicans strains in the same patient with a persistent blood stream infection within a short period of time.
authors.affiliation: Division of Infectious Diseases, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Da Matta
authors.forename: Analy S
authors.initials: AS
authors.lastname: Melo
authors.forename: Thaís
authors.initials: T
authors.lastname: Guimarães
authors.forename: João P
authors.initials: JP
authors.lastname: Frade
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Lott
authors.forename: Arnaldo L
authors.initials: AL
authors.lastname: Colombo
mesh_terms: D001938:Brazil; D002176:Candida albicans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D016469:Fungemia / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D012008:Recurrence; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal