Short communication: characterization of a monoclonal antibody for kappa-casein B of cow's milk.
A monoclonal antibody (antik-B) against an oligopeptide of 23 AA corresponding to the region 131-153 of bovine kappa-casein (kappa-CN) B was generated using the Human Combinatorial Antibody Library (HuCAL) technology. Both AA substitutions distinguishing kappa-CN A and B are located in that region (positions 136 and 148). In this study, the reactivity of antik-B to milk samples collected from cows previously genotyped as CSN3*AA, CSN3*AB, and CSN3*BB was tested. According to Western blot results, antik-B recognized kappa-CN B and it showed no cross-reactivity toward kappa-CN A and other milk proteins. Furthermore, a modified Western blot method, urea-PAGE Western blot, was set up to assess the reactivity of antik-B toward all isoforms of kappa-CN B. In conclusion, antik-B was specific to kappa-CN B in milk and it seemed to be reactive toward all its isoforms.
Summer
A
A
Department of Animal Production, Veterinary Biotechnologies, Food Quality and Safety, University of Parma, via del Taglio 10, 43126 Parma, Italy.
Santus
E
E
Casanova
L
L
Joerg
H
H
Rossoni
A
A
Nicoletti
C
C
Donofrio
G
G
Mariani
P
P
Malacarne
M
M
D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002364:Caseins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005838:Genotype; D008892:Milk / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002364:Caseins