[Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector].
OBJECTIVE
To develop a coexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency.


METHODS
The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA.


RESULTS
Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells.


CONCLUSIONS
The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.
Shen
Hong-Hui
HH
College of Life sciences, Jilin University, Changchun 130012, China.
Liu
Su-Jun
SJ
Guo
Lei
L
Hou
Jun
J
Wang
Zhi-Jie
ZJ
Xin
Shao-Jije
SJ
Bai
Bing-Ke
BK
Hu
Yan
Y
Yu
Xiang-Hui
XH
Kong
Wei
W
Mao
Pan-Yong
PY
D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000235:genetics* / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens