Cytotoxic drug residues in urine of dogs receiving anticancer chemotherapy.
BACKGROUND
The presence of cytotoxic drug residues in urine of dogs may represent an exposure risk for pet owners and other people as well as a potential environmental contaminant. However, studies on cytotoxic drug residues in excretions of clinical patients are lacking in veterinary oncology.


OBJECTIVE
Variable concentrations of cytotoxic residues are present in urine samples, depending on sampling time and substance.


METHODS
Client-owned dogs with lymphoma or mast cell tumors treated with standard chemotherapy protocols.


METHODS
Urine samples were collected before, directly after, and on days after administration of chemotherapy. Measurement of vincristine, vinblastine, cyclophosphamide, and doxorubicin residues in canine urine was performed by a quantitative liquid chromatography tandem mass spectrometry (LC/MS/MS) method.


RESULTS
Median cyclophosphamide residue concentration was 398.2 microg/L directly after treatment (d0) and was below the level of detection on days 1-3 (d1, d2, d3). Median vincristine residue concentration was 53.8 microg/L directly after treatment and was 20.2, 11.4, and 6.6 microg/L on days 1, 2, and 3. Median vinblastine residues were 144.9 (d0), 70.8 (d1), 35.6 (d2), and 18.7 microg/L (d3) with low concentrations detectable for 7 days after treatment. Median urine doxorubicin concentrations were 354.0 (d0), 165.6 (d1), 156.9 (d2), and 158.2 microg/L (d3). Low concentrations of doxorubicin were measurable up to 21 days after administration.


CONCLUSIONS
Variable concentrations of chemotherapeutics were measured in urine samples, depending on sampling time point and drug. Findings may inform current chemoprotection guidelines and help minimize exposure risks.
Knobloch
A
A
Small Animal Hospital, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Mohring
S A I
SA
Eberle
N
N
Nolte
I
I
Hamscher
G
G
Simon
D
D
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000652:urine*; D003520:Cyclophosphamide / Q000652:urine; D004283:Dog Diseases / Q000652:urine*; D004285:Dogs; D004317:Doxorubicin / Q000652:urine; D004350:Drug Residues / Q000032:analysis*; D004781:Environmental Exposure; D008223:Lymphoma / Q000188:drug therapy / Q000662:veterinary*; D012515:Mast-Cell Sarcoma / Q000188:drug therapy / Q000662:veterinary*; D014747:Vinblastine / Q000652:urine; D014750:Vincristine / Q000652:urine
D000970:Antineoplastic Agents; D014750:Vincristine; D014747:Vinblastine; D004317:Doxorubicin; D003520:Cyclophosphamide