Force transmission in migrating cells.
During cell migration, forces generated by the actin cytoskeleton are transmitted through adhesion complexes to the substrate. To investigate the mechanism of force generation and transmission, we analyzed the relationship between actin network velocity and traction forces at the substrate in a model system of persistently migrating fish epidermal keratocytes. Front and lateral sides of the cell exhibited much stronger coupling between actin motion and traction forces than the trailing cell body. Further analysis of the traction-velocity relationship suggested that the force transmission mechanisms were different in different cell regions: at the front, traction was generated by a gripping of the actin network to the substrate, whereas at the sides and back, it was produced by the network's slipping over the substrate. Treatment with inhibitors of the actin-myosin system demonstrated that the cell body translocation could be powered by either of the two different processes, actomyosin contraction or actin assembly, with the former associated with significantly larger traction forces than the latter.
Fournier
Maxime F
MF
Laboratoire de Biophysique Cellulaire, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. maxime.fournier@epfl.ch
Sauser
Roger
R
Ambrosi
Davide
D
Meister
Jean-Jacques
JJ
Verkhovsky
Alexander B
AB
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D005399:Fishes; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D015603:Keratinocytes / Q000502:physiology* / Q000648:ultrastructure; D008954:Models, Biological; D009218:Myosins / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology
D006576:Heterocyclic Compounds, 4 or More Rings; D011500:Protein Synthesis Inhibitors; C472645:blebbistatin; D015638:Cytochalasin D; D009218:Myosins