T-cadherin modulates tumor-associated molecules in gallbladder cancer cells.
T-cadherin is believed to act against carcinogenesis in various tissues; however, its tumor-suppressor mechanism remains largely unclear. Using subtractive mRNA hybridization and immunoblotting, the present study identified several cancer-associated molecules whose expression was modified by T-cadherin in gallbladder cancer cells. Restoration of T-cadherin decreased the expression of Akt3 and phosphorylated Akt molecules. SET7/9, which stabilizes chromatin-bound p53, was downregulated by silencing of T-cadherin but was not regulated by the expression of T-cadherin. These finding suggest that T-cadherin might inhibit tumor progression through multiple pathways, including the Akt and SET7/9-p53 pathways.
Adachi
Yoshihiro
Y
Department of Pathology, Kochi Medical School, Nankoku 783-8505, Japan.
Takeuchi
Tamotsu
T
Nagayama
Tomoko
T
Furihata
Mutsuo
M
D015820:Cadherins / Q000502:physiology*; D045744:Cell Line, Tumor; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D014162:Transfection; D015854:Up-Regulation
D015820:Cadherins; C099972:H-cadherin; D034741:RNA, Small Interfering; D011495:Histone-Lysine N-Methyltransferase; C540312:SETD7 protein, human; C491754:AKT3 protein, human; D051057:Proto-Oncogene Proteins c-akt