title: Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis.
abstract: Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans ( Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans.
authors.affiliation: Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, 1085 Neidong, Miryang 627-803, Korea. taejoung@korea.kr
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
authors.forename: Myoung-Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Park
authors.forename: Suk-Bok
authors.initials: SB
authors.lastname: Pae
authors.forename: Kang-Bo
authors.initials: KB
authors.lastname: Shim
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Ko
authors.forename: Sang-Ouk
authors.initials: SO
authors.lastname: Shin
authors.forename: In-Youl
authors.initials: IY
authors.lastname: Baek
authors.forename: Keum-Yong
authors.initials: KY
authors.lastname: Park
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007887:Fabaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006304:Health Status; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013053:Spectrophotometry; D014675:Vegetables / Q000737:chemistry
chemical_list: D000872:Anthocyanins; C017185:delphinidin