title: Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.
abstract: We have developed dissociable antibody microarray (DAMA) staining technology that provides a new approach to the global analysis of protein subcellular localization (SCL) in fixed cells. We have developed and optimized this technology for protein SCL profiling, generated ChipView, a program for management and analysis of molecular image database, and utilized the technique to identify proteins with unique SCL in breast cancer cell lines. We compared the SCL profiles of 325 proteins among nine different breast cell lines, and have identified one protein, Cyclin B1, with distinctively different SCLs between normal and cancer cell lines. With classic individual immunostaining, Cyclin B1 was confirmed to localize to the cytoplasm of seven breast cancer cell lines and in both cytoplasm and nuclei of two normal breast cell lines, and to have higher expression levels in the cancer cell lines tested.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Guanyuan
authors.initials: G
authors.lastname: Fu
authors.forename: X Cynthia
authors.initials: XC
authors.lastname: Song
authors.forename: Xufen
authors.initials: X
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Wang
authors.forename: G Wayne
authors.initials: GW
authors.lastname: Zhou
mesh_terms: D000906:Antibodies / Q000276:immunology; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D056744:Cyclin B1 / Q000032:analysis; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D000906:Antibodies; C534699:CCNB1 protein, human; D056744:Cyclin B1; D020543:Proteome