Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling.
Gadonanotubes (GNTs), which are powerful new T(1)-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml(-1)) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 x 10(9) Gd(3+) ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T(1)-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.
Hassan
Amy A
AA
Breast Center, Department of Medicine and Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Chan
Bonita Tak-Yee
BT
Tran
Lesa A
LA
Hartman
Keith B
KB
Ananta
Jeyarama S
JS
Mackeyev
Yuri
Y
Hu
Lingyun
L
Pautler
Robia G
RG
Wilson
Lon J
LJ
Lee
Adrian V
AV
D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D048430:Cell Shape; D005260:Female; D005682:Gadolinium / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging / Q000379:methods; D015335:Molecular Probes / Q000378:metabolism*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D019047:Phantoms, Imaging; D012694:Serine / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
D015335:Molecular Probes; D012694:Serine; D005682:Gadolinium