title: Aberrant methylation of DNA-damage-inducible transcript 3 promoter is a common event in patients with myelodysplastic syndrome.
abstract: The epigenetic changes of tumor suppressor genes are now recognized as an alternative mechanism contributing to the development of myelodysplastic syndrome (MDS). The expression of DNA-damage-inducible transcript 3 (DDIT3) gene has been found down-regulated in myeloid malignancies including MDS. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of the CpG island of DDIT3 promoter region in MDS. Aberrant methylation of DDIT3 was detected in 31.8% (21/66) of the cases analyzed. No correlation was found between DDIT3 promoter methylation and clinical parameters and MDS subtypes. Although the estimated 50% survival time of the methylated DDIT3 group was shorter than that of unmethylated group (12.0 months vs. 23.0 months), the difference was not statistically significant (P=0.137). These findings suggest that the hypermethylation of DDIT3 promoter might be one of early events in the development of MDS.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, 212002, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qian
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018899:CpG Islands; D004249:DNA Damage / Q000235:genetics; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015996:Survival Rate; D051742:Transcription Factor CHOP / Q000235:genetics*; D055815:Young Adult
chemical_list: C497249:DDIT3 protein, human; D004273:DNA, Neoplasm; D051742:Transcription Factor CHOP