title: Effects of thawing, refreezing and storage conditions of tissue samples and protein extracts on 2-DE spot intensity.
abstract: We report that reliable quantitative proteome analyses can be performed with tissue samples stored at -80 degrees C for up to 10 years. However, storing protein extracts at 4 degrees C for 24 h and freezing protein extracts at -80 degrees C and thawing them significantly altered 41.6 and 17.5% of all spot intensities on 2-DE gels, respectively. Fortunately, these storing effects did not impair the reliability of quantifying 2-DE experiments. Nonetheless, the results show that freezing and storage conditions should be carefully controlled in proteomic experiments.
authors.affiliation: Department of Radiology and Nuclear Medicine (Radiochemistry), Charité Universitätsmedizin, Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Weist
authors.forename: Cindy
authors.initials: C
authors.lastname: Brunkau
authors.forename: Janosch
authors.initials: J
authors.lastname: Wittke
authors.forename: Murat
authors.initials: M
authors.lastname: Eravci
authors.forename: Oliver
authors.initials: O
authors.lastname: Broedel
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.forename: Heike
authors.initials: H
authors.lastname: Stephanowitz
authors.forename: Selda
authors.initials: S
authors.lastname: Eravci
authors.forename: Andreas
authors.initials: A
authors.lastname: Baumgartner
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005615:Freezing; D008297:Male; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013048:Specimen Handling / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins