Differential diagnosis of feline leukemia virus subgroups using pseudotype viruses expressing green fluorescent protein.
Feline leukemia virus (FeLV) is classified into three receptor interference subgroups, A, B and C. In this study, to differentiate FeLV subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (GFP). We prepared gfp pseudotype viruses, named gfp(FeLV-A), gfp(FeLV-B) and gfp(FeLV-C) harboring envelopes of FeLV-A, B and C, respectively. The gfp pseudotype viruses completely interfered with the same subgroups of FeLV reference strains on FEA cells (a feline embryonic fibroblast cell line). We also confirmed that the pseudotype viruses could differentiate FeLV subgroups in field isolates. The assay will be useful for differential diagnosis of FeLV subgroups in veterinary diagnostic laboratories in the future.
Nakamura
Megumi
M
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Japan.
Sato
Eiji
E
Miura
Tomoyuki
T
Baba
Kenji
K
Shimoda
Tetsuya
T
Miyazawa
Takayuki
T
D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D002415:Cats; D003937:Diagnosis, Differential; D049452:Green Fluorescent Proteins / Q000235:genetics; D007564:Japan; D005256:Leukemia Virus, Feline / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016582:Leukemia, Feline / Q000175:diagnosis*; D014766:Viremia / Q000097:blood / Q000276:immunology / Q000662:veterinary
D000956:Antigens, Viral; D049452:Green Fluorescent Proteins