title: Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration.
abstract: OBJECTIVE
  To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation.
  
  
  METHODS
  We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed.
  
  
  RESULTS
  Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect.
  
  
  CONCLUSIONS
  Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USA. dazar@uic.edu
authors.forename: Dimitri T
authors.initials: DT
authors.lastname: Azar
authors.forename: Fabio H
authors.initials: FH
authors.lastname: Casanova
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Mimura
authors.forename: Sandeep
authors.initials: S
authors.lastname: Jain
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Kyu Yeon
authors.initials: KY
authors.lastname: Han
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Chang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002461:Cell Line, Transformed; D002465:Cell Movement; D049109:Cell Proliferation; D004730:Endothelium, Vascular / Q000166:cytology*; D019573:Epithelium, Corneal / Q000201:enzymology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D053511:Matrix Metalloproteinase 14 / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011651:Pulmonary Artery / Q000166:cytology; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
chemical_list: D020533:Angiogenesis Inhibitors; C507096:Mmp14 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D053511:Matrix Metalloproteinase 14