Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of Candida glabrata.
A Candida glabrata calcineurin mutant exhibited increased susceptibility to both azole antifungal and cell wall-damaging agents and was also attenuated in virulence. Although a mutant lacking the downstream transcription factor Crz1 displayed a cell wall-associated phenotype intermediate to that of the calcineurin mutant and was modestly attenuated in virulence, it did not show increased azole susceptibility. These results suggest that calcineurin regulates both Crz1-dependent and -independent pathways depending on the type of stress.
Miyazaki
Taiga
T
Department of Molecular Microbiology and Immunology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. taiga-m@nagasaki-u.ac.jp
Yamauchi
Shunsuke
S
Inamine
Tatsuo
T
Nagayoshi
Yosuke
Y
Saijo
Tomomi
T
Izumikawa
Koichi
K
Seki
Masafumi
M
Kakeya
Hiroshi
H
Yamamoto
Yoshihiro
Y
Yanagihara
Katsunori
K
Miyazaki
Yoshitsugu
Y
Kohno
Shigeru
S
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D041221:Candida glabrata / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009894:Opportunistic Infections / Q000188:drug therapy / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014774:Virulence / Q000235:genetics / Q000502:physiology
D000935:Antifungal Agents; D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D014157:Transcription Factors; D019703:Calcineurin