Designing hyaluronic acid-based layer-by-layer capsules as a carrier for intracellular drug delivery.
Polyelectrolyte microcapsules were prepared by the layer-by-layer assembly of hyaluronic acid (HA) and a polycationic polymer, poly(allylamine) (PAH) or poly(lysine) (PLL). The influence of the polycationic partner on the morphology, stability, permeability properties, and enzymatic degradation of microcapsules was thoroughly analyzed. It was found that these properties could be tuned by shell cross-linking. Confocal microscopy studies of cellular uptake of the capsules showed that the polyelectrolyte shells remain stable outside the cells but readily break open once internalized by cells, suggesting their potential as carrier for intracellular drug delivery.
Szarpak
Anna
A
Centre de Recherches sur les Macromolecules Vegetales-CNRS, BP53, 38041 Grenoble cedex 9, France.
Cui
Di
D
Dubreuil
Frédéric
F
De Geest
Bruno G
BG
De Cock
Liesbeth J
LJ
Picart
Catherine
C
Auzély-Velty
Rachel
R
D000818:Animals; D002151:Calorimetry / Q000379:methods; D002214:Capsules; D002460:Cell Line; D004337:Drug Carriers; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning
D002214:Capsules; D004337:Drug Carriers; D006820:Hyaluronic Acid