Metabolic programming of lipid profile and reproductive organs weight by leptin treatment on early life.
OBJECTIVE
To evaluate whether the neonatal leptin treatment during the first days of life can program the male reproductive organs weight and the lipid profile.


METHODS
At birth 6 dams were divided into 2 groups: Leptin - each pup was injected with 50microL of recombinant rat leptin (80ng/g BW, sc), for the first 10 d of lactation; Control - each pup received the same volume of saline. After weaning, all pups received unlimited access to food until 190 days of age when they were killed. Values are given as mean + or - SEM of 6 animals and Test t Student was used to analyze the results.


RESULTS
The leptin treatment resulted in a significant increase in body weight (Control= 411.8 + or - 16.31; Leptin= 481.8 + or - 11.29, p=0.005) and food consumption (Control= 25.32 + or - 0.09; Leptin= 32.42 + or - 0.15, p=0.0001) and a significant reduction in triglycerides levels (Control= 540.0 + or - 117.9; Leptin= 93.25 + or - 15.21, p=0.006) and in the weight of hypothalamus (Control= 0.234 + or - 0.016; Leptin= 0.154 + or - 0.015, p=0.007), pituitary (Control= 0.104 + or - 0.0120; Leptin= 0.033 + or - 0.012, p=0.003), testis (Control= 3.75 + or - 0.055; Leptin= 3.19 + or - 0.10, p=0.002) and prostate (Control=1.641 + or - 0.1389; Leptin= 0.91 + or - 0.07, p=0.001).


CONCLUSIONS
Leptin treatment on the first days of life can program the reproductive organs weight and the lipid profile of the progeny.
Marques
Durval Santos
DS
Urogenital Research Unit, UERJ, Rio de Janeiro, Brazil.
Gombar
Flávia Meireles
FM
Pereira
Jorge Luiz Alves
JL
Sampaio
Francisco José Barcellos
FJ
Ramos
Cristiane da Fonte
Cda F
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling / Q000502:physiology; D005837:Genitalia, Male / Q000033:anatomy & histology / Q000187:drug effects*; D020738:Leptin / Q000494:pharmacology*; D008055:Lipids / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D020738:Leptin; D008055:Lipids