Genistein and daidzein act on a panel of genes implicated in cell cycle and angiogenesis by polymerase chain reaction arrays in human prostate cancer cell lines.
BACKGROUND
The prostate cancer most frequently affects men. The ethnic origin and family antecedents of prostate cancer are established as risk factors. The genetic factors associated with environmental factors such as the nutrition also play a role in the development of the cancer. Epidemiological studies showed that the Asian populations exhibited an incidence of prostate cancer markedly subordinate by comparison with the Western populations. This would be explained partially by their important consumption of soy. Both main phytoestrogens of soy, the genistein and the daidzein, present anti-proliferative properties.


METHODS
For that purpose, we used different prostate cancer cell lines (LNCaP, DU 145, PC-3) and, by flow cytometry, we determined the concentration of phytoestrogens inducing a cell cycle arrest and the required time of incubation.


RESULTS
Then, the effects of 40microM genistein or 110microM daidzein for 48h were determined and studied on the expression of genes involved in the human cell cycle and angiogenesis and conducted by SYBR green quantitative PCR.


CONCLUSIONS
We demonstrated modulations of cyclin-dependent kinase-related pathway genes, DNA damage-signaling pathway and a down-regulation of EGF and IGF.
Rabiau
Nadège
N
Centre Jean Perrin, Department of Oncogenetics, EA 4233, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P. 38, 63011 Clermont-Ferrand, France.
Kossaï
Myriam
M
Braud
Martin
M
Chalabi
Nasséra
N
Satih
Samir
S
Bignon
Yves-Jean
YJ
Bernard-Gallon
Dominique J
DJ
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D009389:Neovascularization, Pathologic / Q000235:genetics; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism; D013002:Somatomedins / Q000235:genetics
D016588:Anticarcinogenic Agents; D007529:Isoflavones; D013002:Somatomedins; D004815:Epidermal Growth Factor; C004742:daidzein; D019833:Genistein