Drosophila octamer elements and Pdm-1 dictate the coordinated transcription of core histone genes.
We reveal a set of divergent octamer elements in Drosophila melanogaster (dm) core histone gene promoters. These elements recruit transcription factor POU-domain protein in D. melanogaster 1 (Pdm-1), which along with co-activator dmOct-1 coactivator in S-phase (dmOCA-S), activates transcription from at least the Drosophila histone 2B (dmH2B) and 4 (dmH4) promoters in a fashion similar to the transcription of mammalian histone 2B (H2B) gene activated by octamer binding transcription factor 1 (Oct-1) and Oct-1 coactivator in S-phase (OCA-S). The expression of core histone genes in both kingdoms is coordinated; however, although the expression of mammalian histone genes involves subtype-specific transcription factors and/or co-activator(s), the expression of Drosophila core histone genes is regulated by a common module (Pdm-1/dmOCA-S) in a directly coordinated manner. Finally, dmOCA-S is recruited to the Drosophila histone locus bodies in the S-phase, marking S-phase-specific transcription activation of core histone genes.
Lee
Mei-Chin
MC
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore.
Toh
Ling-Ling
LL
Yaw
Lai-Ping
LP
Luo
Yan
Y
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D005434:Flow Cytometry; D006657:Histones / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D050809:POU Domain Factors / Q000378:metabolism*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D016196:S Phase; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D029721:Drosophila Proteins; D006657:Histones; D018398:Homeodomain Proteins; D050809:POU Domain Factors; D012330:RNA, Double-Stranded; C104121:nub protein, Drosophila; D003850:Deoxyribonuclease I