title: Carboxylated calixarenes bind strongly to CD69 and protect CD69(+) killer cells from suicidal cell death induced by tumor cell surface ligands.
abstract: We have recently identified a new class of high affinity ligands for CD69 leukocyte membrane receptor, carboxylated calixarenes. Of the three compounds investigated here, thiacalix[4]arene had the highest affinity for CD69 in direct binding assays, and proved to be the most specific inhibitor of CD69 identified so far in receptor precipitation and cellular activation experiments. Carboxylated calixarenes also proved effective at protection of CD69(high) lymphocytes from apoptosis triggered by a multivalent ligand or antibody. Thus, carboxylated calixarenes set a new paradigm for noncarbohydrate ligands for CD69 making them attractive for protection of killer cells in combined animal tumor therapies.
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University Prague, Czech Republic. bezouska@biomed.cas.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Bezouska
authors.forename: Renata
authors.initials: R
authors.lastname: Snajdrová
authors.forename: Karel
authors.initials: K
authors.lastname: Krenek
authors.forename: Markéta
authors.initials: M
authors.lastname: Vancurová
authors.forename: Alan
authors.initials: A
authors.lastname: Kádek
authors.forename: David
authors.initials: D
authors.lastname: Adámek
authors.forename: Pavel
authors.initials: P
authors.lastname: Lhoták
authors.forename: Daniel
authors.initials: D
authors.lastname: Kavan
authors.forename: Katerina
authors.initials: K
authors.lastname: Hofbauerová
authors.forename: Petr
authors.initials: P
authors.lastname: Man
authors.forename: Pavla
authors.initials: P
authors.lastname: Bojarová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kren
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D017209:Apoptosis; D047250:Calixarenes / Q000737:chemistry / Q000378:metabolism*; D002264:Carboxylic Acids / Q000737:chemistry*; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D051381:Rats; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D047250:Calixarenes; D002264:Carboxylic Acids; D037181:Lectins, C-Type; D008024:Ligands; D000099192:CD69 Antigens