Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data.
We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood.
Marullo
Manuela
M
Department of Pharmacological Sciences and Center for Stem Cell Research, University of Milan, 9 Via Balzaretti, Milan, 20133, Italy. manuela.marullo@unimi.it
Zuccato
Chiara
C
Mariotti
Caterina
C
Lahiri
Nayana
N
Tabrizi
Sarah J
SJ
Di Donato
Stefano
S
Cattaneo
Elena
E
D000328:Adult; D020087:Alu Elements; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D008957:Models, Genetic; D018482:Muscle, Skeletal / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D001613:beta 2-Microglobulin / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D012313:RNA