title: Investigation of the oviductal glycoprotein 1 (OVGP1) gene associated with embryo survival and development in the rabbit.
abstract: An association study was performed in rabbits between early embryo survival and development, and the nonconservative SNP 12944C>G located in exon 11 and the triallellic microsatellite [(GT)(15)T(G)(5), (GT)(14)T(G)(5), and (GT)(11)T(G)(7))] located in the promoter region of the oviductal glycoprotein 1 (OVGP1) gene. We analyzed an F(2) cross of 2 lines of rabbits divergently selected for uterine capacity. A total of 172 and 159 females were slaughtered at 48 and 72 h of gestation, respectively, to determine whether OVGP1 influences ovulation rate, fertilization rate, early embryo survival, and embryonic stage of development. The results of the SNP indicated that all genotypes showed similar early embryo survival and a similar embryonic stage of development at 48 h of gestation. However, at 72 h of gestation, the GG genotype showed greater early embryo survival than the CC genotype (0.56 embryos) and their embryos presented less embryonic development. Analysis of the microsatellite was performed to ascertain the presence or absence of the allele (GT)(14)T(G)(5). At both stages of gestation, the (GT)(14)T(G)(5)/(GT)(14)T(G)(5) genotype showed greater early embryo survival (0.94 and 1.54 embryos at 48 and 72 h of gestation, respectively) and less embryonic development than the homozygous genotypes without the allele (GT)(14)T(G)(5).
authors.affiliation: Departamento de Tecnología Agroalimentaria, Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain. mariluz.garcia@umh.es
authors.forename: M L
authors.initials: ML
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: Peiró
authors.forename: M J
authors.initials: MJ
authors.lastname: Argente
authors.forename: M
authors.initials: M
authors.lastname: Merchán
authors.forename: J M
authors.initials: JM
authors.lastname: Folch
authors.forename: A
authors.initials: A
authors.lastname: Blasco
authors.forename: M A
authors.initials: MA
authors.lastname: Santacreu
mesh_terms: D000483:Alleles; D000818:Animals; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006720:Homozygote; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011817:Rabbits
chemical_list: D006023:Glycoproteins