Novel protein ligands of the annexin A7 N-terminal region suggest pro-beta helices engage one another with high specificity.
The N-terminal regions of annexins A7 (synexin) and A11 consist of an extended series of short sequence repeats rich in tyrosine, proline, and glycine that provide binding sites for other proteins that may be recruited to membranes by the annexins and that may modulate the calcium and membrane binding activities of the annexin core domains. In this study two new ligands for the annexin A7 N terminal region were identified by yeast two hybrid screening: the TNFalpha receptor regulatory protein SODD (Suppressor Of Death Domains) and KIAA0280, a protein of unknown function. Strikingly, the sites of interaction of these proteins with the annexin also contain sequence repeats similar to those present in the N-termini of annexins A7 and A11. It was also found that the annexin A7 N-terminal region interacts with itself in the two hybrid assay. These results suggest that sequence repeats of this nature form novel structures, called YP pro-beta helices, that are characterized by an ability to interact with one another. Specificity of interactions between the pro-beta helices in different proteins may be encoded by the variations of residues and lengths of the sequence repeats.
Creutz
Carl E
CE
Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA. Creutz@virginia.edu
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017310:Annexin A7 / Q000737:chemistry* / Q000378:metabolism; D017302:Annexins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D015723:Gene Library; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020798:Two-Hybrid System Techniques
D017310:Annexin A7; D017302:Annexins; D008024:Ligands