title: Identification of thioredoxin target disulfides in proteins released from barley aleurone layers.
abstract: Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the starchy endosperm. Here we have applied a thiol-specific labeling approach to identify specific disulfide targets of barley thioredoxin in proteins released from barley aleurone layers incubated in buffer containing gibberellic acid.
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Søltofts Plads, Building 224, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. ph@bio.dtu.dk
authors.forename: Per
authors.initials: P
authors.lastname: Hägglund
authors.forename: Jakob
authors.initials: J
authors.lastname: Bunkenborg
authors.forename: Fen
authors.initials: F
authors.lastname: Yang
authors.forename: Lea Mørch
authors.initials: LM
authors.lastname: Harder
authors.forename: Christine
authors.initials: C
authors.lastname: Finnie
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
mesh_terms: D002021:Buffers; D004247:DNA / Q000737:chemistry; D004220:Disulfides; D056625:Endosperm / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination; D005875:Gibberellins / Q000737:chemistry; D001467:Hordeum / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry*
chemical_list: D002021:Buffers; D004220:Disulfides; D005875:Gibberellins; D010940:Plant Proteins; D013879:Thioredoxins; D004247:DNA; C007842:gibberellic acid