title: Separate and combined effects of caffeine and dbcAMP on olive baboon (Papio anubis) sperm.
abstract: BACKGROUND
  Improvement of baboon sperm capacitation is necessary for achieving high in vitro fertilization (IVF) rates in baboons. In this study, we evaluated separate and combined effects of caffeine and dbcAMP on baboon sperm capacitation.
  
  
  METHODS
  Sixteen male baboons (n = 16) were electroejaculated. Each sperm sample was divided into two aliquots: one for chemical activation and the other untreated control. Group 1: dbcAMP (n = 6); Group 2: caffeine (n = 6) and Group 3: combination of caffeine and dbcAMP (n = 4). In each aliquot, sperm motility after 30 minutes of incubation was evaluated as well as zona pellucida (ZP) binding ability after overnight incubation with 4-5 ZP from unfertilized human oocytes.
  
  
  RESULTS
  Sperm motility and ZP binding ability in all chemically activated groups increased significantly as compared to their respective controls (P < 0.05).
  
  
  CONCLUSIONS
  Combined and separate effects of caffeine and dbcAMP increases baboon sperm motility and ZP binding ability and may improve baboon IVF.
authors.affiliation: Leuven University Fertility Centre, University Hospital Gasthuisberg, Leuven, Belgium.
authors.forename: Atunga
authors.initials: A
authors.lastname: Nyachieo
authors.forename: Carl
authors.initials: C
authors.lastname: Spiessens
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Chai
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Kiulia
authors.forename: Jason M
authors.initials: JM
authors.lastname: Mwenda
authors.forename: Thomas M
authors.initials: TM
authors.lastname: D'Hooghe
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D005307:Fertilization in Vitro / Q000187:drug effects; D008297:Male; D048530:Papio anubis; D013075:Sperm Capacitation / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D002110:Caffeine; D003994:Bucladesine