title: In vivo tracking of mesenchymal stem cells labeled with a novel chitosan-coated superparamagnetic iron oxide nanoparticles using 3.0T MRI.
abstract: This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl(2) and FeCl(3). The human bone marrow derived mesenchymal stem cells (hBM-MSC) were labeled with 50 microg Fe/mL chitosan-SPIO and Resovist. The labeling efficiency was assessed by iron content, Prussian blue staining, electron microscopy and in vitro MR imaging. The labeled cells were also analyzed for cytotoxicity, phenotype and differentiation potential. Electron microscopic observations and Prussian blue staining revealed 100% of cells were labeled with iron particles. MR imaging was able to detect the labeled MSC successfully. Chitosan-SPIO did not show any cytotoxicity up to 200 microg Fe/mL concentration. The labeled stem cells did not exhibit any significant alterations in the surface markers expression or adipo/osteo/chondrogenic differentiation potential when compared to unlabeled control cells. After contralateral injection into rabbit ischemic brain, the iron labeled stem cells were tracked by periodical in vivo MR images. The migration of cells was also confirmed by histological studies. The novel chitosan-SPIO enables to label and track MSC for in vivo MRI without cellular alteration.
authors.affiliation: Department of Radiology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Alavala Matta
authors.initials: AM
authors.lastname: Reddy
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Kwak
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Shim
authors.forename: Chiyoung
authors.initials: C
authors.lastname: Ahn
authors.forename: Hyo Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Yong Jae
authors.initials: YJ
authors.lastname: Suh
authors.forename: Eon Sub
authors.initials: ES
authors.lastname: Park
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000139:chemically induced / Q000473:pathology / Q000628:therapy; D002454:Cell Differentiation; D048271:Chitosan / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry* / Q000633:toxicity; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology; D053768:Metal Nanoparticles / Q000737:chemistry*; D010641:Phenotype; D011817:Rabbits
chemical_list: D056831:Coordination Complexes; D005290:Ferric Compounds; C000499:ferric oxide; D048271:Chitosan