Granulocyte-colony stimulating factor treatment of chronic myocardial infarction.
OBJECTIVE
The aim of this study was to investigate the impact of granulocyte-colony stimulating factor (G-CSF) administration on cardiac function of rats with chronic myocardial infarction through two different protocols: high dose short term and low dose long term protocols.


METHODS
Wistar rats were submitted to MI surgery and after 4 weeks they received recombinant human G-CSF (Filgrastim) or vehicle subcutaneously. We tested the classical protocol (50 microg/kg/day during 7 days) and the long term low dose treatment (four cycles of 5 days of 10 microg/kg/day). Cardiac performance was evaluated before, 4 and 6 weeks after G-CSF injections by electro- and echocardiography, hemodynamic and treadmill exercise test.


RESULTS
All infarcted groups exhibited impaired function compared to sham operated animals. Moreover, all cardiac functional parameter were not different between G-CSF and Vehicle group at resting conditions as well as after treadmill exercise stress test, despite intense white blood cell mobilization in both protocols at all time points. Hypertrophy was not different and infarct size was similar in histological analysis


CONCLUSIONS
These data clearly show that G-CSF treatment was unable to restore cardiac function impaired by myocardial infarction either with classical approach or long term low dose administration.
Louzada
Ruy A N
RA
Laboratório de Cardiologia Celular e Molecular do Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, CEP 21941-902, Brasil.
Oliveira
Patricia F
PF
Cavalcanti-de-Albuquerque
Joao Paulo A
JP
Cunha-Carvalho
Leandro
L
Baldanza
Marcelo R
MR
Kasai-Brunswick
Taís H
TH
Goldenberg
Regina C S
RC
Campos-de-Carvalho
Antonio C
AC
Werneck-de-Castro
Joao P S
JP
D000818:Animals; D001769:Blood / Q000187:drug effects; D001794:Blood Pressure; D002452:Cell Count; D004452:Echocardiography; D004562:Electrocardiography; D005080:Exercise Test; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D006098:Granulocytes / Q000166:cytology; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006439:Hemodynamics / Q000502:physiology; D007958:Leukocyte Count; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D018487:Ventricular Dysfunction, Left / Q000473:pathology / Q000503:physiopathology
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim