title: Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes.
abstract: The avian influenza viruses (AIVs) can be highly contagious to poultry and a zoonotic threat to humans. Since the memory CD8(+) T lymphocyte responses in chickens to AIV proteins have not been defined, these responses to H5N9 AIV hemagglutinin (HA) and nucleocapsid (NP) proteins were evaluated by ex vivo stimulation with virus infected non-professional antigen presenting cells. Secretion of IFNgamma by activated T lymphocytes was evaluated through macrophage induction of nitric oxide. AIV specific, MHC-I restricted memory CD8(+) T lymphocyte responses to NP and HA were observed 3 to 9 weeks post-inoculation (p.i.). The responses specific to NP were greater than those to HA with maximum responses being observed at 5 weeks p.i. followed by a decline to weakly detectable levels by 9 weeks p.i. The cross-reaction of T lymphocytes to a heterologous H7N2 AIV strain demonstrated their ability to respond to a broader range of AIV.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical, Sciences, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Shailbala
authors.initials: S
authors.lastname: Singh
authors.forename: Worthie E
authors.initials: WE
authors.lastname: Briles
authors.forename: Blanca
authors.initials: B
authors.lastname: Lupiani
authors.forename: Ellen W
authors.initials: EW
authors.lastname: Collisson
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology / Q000821:virology; D003429:Cross Reactions; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology* / Q000821:virology; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D019590:Nucleocapsid Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma