title: Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats.
abstract: OBJECTIVE
  To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats.
  
  
  METHODS
  Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number.
  
  
  RESULTS
  After 16 weeks, mean of apoptotic cells number in control group (19,3 + or - 3,2) was greater than 40mg/Kg group (11,8 + or - 1,9; p<0,01) and 80mg/Kg group (7,0 + or - 1,4; p<0,01). Significant difference also observed between 40mg/Kg and 80mg/Kg (p<0,05). After 18 weeks, mean of apoptotic cells number in control group (18,0 + or - 2,2) was greater than 40mg/Kg group (8,8 + or - 1,7; p<0,01) and 80mg/Kg group (5,5 + or - 1,3; p<0,01). Significant difference wasn't observed between 40mg/Kg and 80mg/Kg (ns).
  
  
  CONCLUSIONS
  Intra-pulmonary instillation of Benzo[a]pyrene induces significant decrease of apoptotic activity in lung tissue.
authors.affiliation: Central West Region, UFMS, MS, Brazil. ftbaldock@uol.com.br
authors.forename: Baldomero Antonio Kato da
authors.initials: BA
authors.lastname: Silva
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Daniel Martins
authors.initials: DM
authors.lastname: Pereira
authors.forename: Paulo de Tarso Camillo de
authors.initials: Pde T
authors.lastname: Carvalho
authors.forename: Doroty Mesquita
authors.initials: DM
authors.lastname: Dourado
authors.forename: Filipe Abdalla dos
authors.initials: FA
authors.lastname: Reis
authors.forename: Renato Silva
authors.initials: RS
authors.lastname: Nacer
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000008:administration & dosage*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001564:Benzo(a)pyrene