Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media.
Poor compatibility was the major drawback of polymer mixtures when used as DNA separation media. Using poly(ethylene oxide)-b-poly(N, N-dimethylacrylamide) (PEO(44)-b-PDMA(88)) and PEO (M(w): 1.3 MDa) as an example, we demonstrated the concept that the compatibility was significantly improved when mixing a homopolymer with its copolymer. Laser light scattering indicated that the major interaction between PEO and PEO-b-PDMA in dilute solution was the weak hydrodynamic interaction, which showed almost no effect on the viscosity and the static scattering pattern. In semidilute or concentrated solution, viscosity measurement also suggested good compatibility between the two components. When tested as DNA separation medium by CE, the viscosity of the mixture was extremely low, only 5 cP for 5.0 m/v% PEO-b-PDMA+0.1 m/v% PEO at 25 degrees C. The performance on DNA separation could be tuned by varying the concentration of each component as well as the component ratio. Good separation on both dsDNA and ssDNA was achieved.
Ke
Fuyou
F
Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, PR China.
Mo
Xiulei
X
Yang
Runmiao
R
Wang
Yanmei
Y
Liang
Dehai
D
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008689:Methacrylates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012542:Scattering, Radiation; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014783:Viscosity
D008689:Methacrylates; D012996:Solutions; C521514:poly(ethylene oxide)-block-poly(2-((dimethylamino)ethyl methacrylate)); D011092:Polyethylene Glycols; D004247:DNA