[Expression of the cold receptor TRPM8 in the smooth muscles of the seminal ejaculatory ducts in rats].
By means of real-time RT-PCR and immunofluorscent staining combined with confocal microscopy we show for the first time the expression ofmRNA and protein ofthe cold/menthol-sensitive cationic channel, TRPM8, in the smooth muscle cells (SMC) from the epididimal and prostatic portions of the rat vas deferens. Expression of TRPM8 mRNA correlated with the expression ofmRNA for androgen receptor (AR): orchidectomy of the animals resulted in the enhancement of the expression of both mRNAs, which likely reflects specific for the vas deferens compensatory response to the decreasing levels of circulating androgens. TRPM8 protein in the SMC from both parts of the vas deferens primarily localized outside the plasma membrane (PM), however, in the SMC from prostatic portion we observed higher TRPM8 protein targeting specifically the endoplasmic reticulum and PM, where it can form functional cold/menthol-sensitive cationic channel capable of modulating contractile activity of the smooth muscle.
Boldyrev
O I
OI
Sotkis
H V
HV
Kuliieva
Ie M
IeM
Vladymyrova
I A
IA
Filippov
I B
IB
Skryma
R
R
Prevars'ka
N
N
Shuba
Ia M
IaM
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000502:physiology; D004822:Epididymis / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000378:metabolism* / Q000502:physiology; D009919:Orchiectomy; D011467:Prostate / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050053:TRPM Cation Channels / Q000096:biosynthesis*; D014649:Vas Deferens / Q000378:metabolism* / Q000502:physiology
D050053:TRPM Cation Channels; C498947:Trpm8 protein, rat