[Expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and their significance].
OBJECTIVE
To observe the expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and explore their relationship with the metastasis of human prostate cancer.


METHODS
The expressions and distribution of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines (IF11 and IA8) were detected by Western blot and immunofluorescent staining.


RESULTS
The expression of E-cadherin was high in LNCaP, but absent in IF11 and IA8, while beta-catenin was expressed highly in IF11 and LA8, but lowly in LNCaP. Immunofluorescent staining showed that E-cadherin was mainly in the membrane of LNCaP, while beta-catenin both in the membrane of LNCaP and in the nuclei of IF11 and IA8.


CONCLUSIONS
E-cadherin and beta-catenin are differently expressed and distributed in prostate cancer cell lines with different characteristics of epithelial-mesenchymal transition (EMT), and the abnormal activation of the beta-catenin signal pathway may be involved in the EMT of prostate cancer cells.
Jiang
Yong-guang
YG
Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beiing 100029, China. yongguangjiang2008@yahoo.com.cn
Zhao
Jia-hui
JH
Luo
Yong
Y
He
Da-lin
DL
Li
Nan
N
Cui
Xin-hao
XH
Peng
Tao
T
D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism*
D015820:Cadherins; D051176:beta Catenin