[Isolation and identification of Banna virus from mosquito for the first time in Inner Mongolia].
OBJECTIVE
To identify the virus isolated from a mosquito Culex modestus collected from Tongliao city of Inner Mongolia Autonomous Region.


METHODS
A strain of virus isolated from mosquito in Tongliao city was identified by serological and molecular biological methods. The nucleotides of the virus isolate were amplified by RT-PCR, and the products were purified and sequenced. Multiple alignment, phylogenetic and amino acid (AA) analysis were carried out by software Clustal X, MEGA4 and MegAlign (DNAStar).


RESULTS
The new isolate was identified to be Banna virus by serological and molecular biological methods. Phylogenetic analysis showed that the Chinese isolates were distributed within one cluster. The homologue of nucleotide and amino acid of 12 segments between the new isolate and other strains isolated from China were 89.6%-98.4% and 90.4%-98.6%.


CONCLUSIONS
The virus isolated from Culex modestus in Inner Mongolia belonged to Banna virus, and it is the first time that Banna virus was isolated in this region.
Cao
Yu-xi
YX
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Fu
Shi-hong
SH
Tian
Zhao-feng
ZF
He
Ying
Y
Wang
Huan-qin
HQ
Wang
Huan-yu
HY
Yang
Hong-mei
HM
Tao
Bo
B
Liang
Guo-dong
GD
D000818:Animals; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D009033:Culicidae / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012088:Reoviridae Infections / Q000276:immunology / Q000821:virology