A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.
BACKGROUND
Pancreas oxygenation during cold storage has been established in islet isolation and transplantation to prevent ischemic tissue damage using perfluorodecalin (PFD) as hyperoxygen carrier. However, studies in humans and pigs provided conflicting results about the efficiency of PFD for pancreas oxygenation. The aim of this study was to compare PFD with a newly developed oxygen carrier composed of perfluorohexyloctane and polydimethylsiloxane 5 (F6H8S5) for long-term storage of human pancreata.


METHODS
After 24-hr storage in preoxygenated PFD or F6H8S5, pancreata were processed using Liberase HI for pancreas dissociation and a Ficoll gradient for islet purification. Islet quality assessment was performed measuring glucose-stimulated insulin release, viability, islet ATP content, and posttransplant function in diabetic nude mice.


RESULTS
Compared with PFD, F6H8S5 significantly increased the intrapancreatic partial oxygen pressure and islet ATP content. This corresponded to an increase of islet yield, recovery after culture, glucose stimulation index, viability, and improved graft function in diabetic nude mice.


CONCLUSIONS
The present findings indicate clearly that F6H8S5 improves isolation outcome after prolonged ischemia compared with PFD. This observation seems to be related to the significant lipophilicity and almost pancreas-specific density of F6H8S5. Moreover, these characteristics facilitate pancreas shipment without using custom-made transport vessels as required for PFD.
Brandhorst
Heide
H
Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden. Daniel.Brandhorst@klinimm.uu.se
Asif
Sana
S
Andersson
Karin
K
Theisinger
Bastian
B
Andersson
Helene H
HH
Felldin
Maria
M
Foss
Aksel
A
Salmela
Kaija
K
Tibell
Annika
A
Tufveson
Gunnar
G
Korsgren
Olle
O
Brandhorst
Daniel
D
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D005466:Fluorocarbons / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D010100:Oxygen / Q000032:analysis; D010179:Pancreas; D013552:Swine; D014019:Tissue Donors
D001802:Blood Substitutes; D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; C008805:perfluorodecalin; D010100:Oxygen