Glycan and lectin microarrays for glycomics and medicinal applications.
Three different array formats to study a challenging field of glycomics are presented here, based on the use of a panel of immobilized glycan or lectins, and on in silico computational approach. Glycan and lectin arrays are routinely used in combination with other analytical tools to decipher a complex nature of glycan-mediated recognition responsible for signal transduction of a broad range of biological processes. Fundamental aspects of the glycan and lectin array technology are discussed, with the focus on the choice and availability of the biorecognition elements, fabrication protocols, and detection platforms involved. Moreover, practical applications of both technologies especially in the field of clinical diagnostics are provided. The future potential of a complementary in silico array technology to reveal details of the protein-glycan-binding profiles is discussed here.
Katrlík
Jaroslav
J
Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Svitel
Juraj
J
Gemeiner
Peter
P
Kozár
Tibor
T
Tkac
Jan
J
D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D002460:Cell Line; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D054794:Glycomics / Q000379:methods*; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000737:chemistry*; D008024:Ligands; D008958:Models, Molecular; D009369:Neoplasms / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D015398:Signal Transduction
D002241:Carbohydrates; D000939:Epitopes; D037102:Lectins; D008024:Ligands; D011134:Polysaccharides