title: Receptor changes in brain tissue of rats treated as neonates with capsaicin.
abstract: Capsaicin, the hot chemical in chillies, administered to neonatal rats, causes destruction of polymodal nociceptive primary afferent neurons by acting on TRPV1 receptors causing intrinsic somatosensory deprivation. Although the effects of neonatal capsaicin treatment in the periphery have been extensively investigated, less is known about the brain networks to which the capsaicin sensory neurons are relayed. In the present study the effect of neonatal capsaicin treatment on brain receptors that have been shown to interact with TRPV1 was examined. Wistar rats were treated on neonatal day 2 with capsaicin and at 15-16 weeks of age, brains were processed to measure levels of muscarinic M(1)/M(2) and M(2)/M(4), serotonin 5HT(2A), cannabinoid CB(1), dopamine D(1), D(2) receptors and dopamine transporter. Overall increases in levels of muscarinic M(1)/M(4) (F=8.219, df=1, p=0.005), muscarinic M(2)/M(4) (F=99.759, df=1, p<0.0001), serotonin 5HT(2A) (F=28.892, df=1, p<0.0001), dopamine D(1) (F=8.726, df=1, p=0.008) and cannabinoid CB(1) (F=25.084, df=1, p<0.0001) receptors were found in the brains of capsaicin-treated rats, although significant regional changes occurred only in muscarinic M(2)/M(4) and serotonin 5HT(2A) receptors. The results of the present study suggest that neonatal intrinsic somatosensory deprivation may have a significant impact on substrates at the central nervous system that manifest as changes in central cholinergic, monaminergic and cannabinoid systems in the adult animal.
authors.affiliation: Radiopharmaceutical Research Institute, Australian Nuclear Science and Technology Organization (ANSTO), Menai, New South Wales, Australia. katerina.zavitsanou@ansto.gov.au <katerina.zavitsanou@ansto.gov.au>
authors.forename: Katerina
authors.initials: K
authors.lastname: Zavitsanou
authors.forename: Victoria S
authors.initials: VS
authors.lastname: Dalton
authors.forename: Hongqin
authors.initials: H
authors.lastname: Wang
authors.forename: Penny
authors.initials: P
authors.lastname: Newson
authors.forename: Loris A
authors.initials: LA
authors.lastname: Chahl
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects* / Q000254:growth & development / Q000503:physiopathology; D063385:Cannabinoid Receptor Modulators / Q000378:metabolism; D002211:Capsaicin / Q000633:toxicity*; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000187:drug effects / Q000378:metabolism; D005260:Female; D008297:Male; D009415:Nerve Net / Q000187:drug effects* / Q000254:growth & development / Q000503:physiopathology; D010146:Pain / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000187:drug effects / Q000378:metabolism; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000187:drug effects* / Q000378:metabolism; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism; D012683:Sensory Deprivation / Q000502:physiology; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism; D018689:Sensory System Agents / Q000633:toxicity; D012701:Serotonin / Q000378:metabolism; D050916:TRPV Cation Channels / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D063385:Cannabinoid Receptor Modulators; D050483:Dopamine Plasma Membrane Transport Proteins; D043884:Receptor, Cannabinoid, CB1; D011954:Receptors, Dopamine; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; D011985:Receptors, Serotonin; D018689:Sensory System Agents; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D012701:Serotonin; D000109:Acetylcholine; D002211:Capsaicin; D004298:Dopamine