Genome-wide screening of genes whose enhanced expression affects glycogen accumulation in Escherichia coli.
Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.
Eydallin
Gustavo
G
Instituto de Agrobiotecnología, Universidad Pública de Navarra/Gobierno de Navarra/Consejo Superior de Investigaciones Científicas, Mutiloako etorbidea zenbaki gabe, Mutiloabeiti, Nafarroa, Spain.
Montero
Manuel
M
Almagro
Goizeder
G
Sesma
María Teresa
MT
Viale
Alejandro M
AM
Muñoz
Francisco José
FJ
Rahimpour
Mehdi
M
Baroja-Fernández
Edurne
E
Pozueta-Romero
Javier
J
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006003:Glycogen / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism
D029968:Escherichia coli Proteins; D006003:Glycogen; D009584:Nitrogen