title: A multi-parametric islet perifusion system within a microfluidic perifusion device.
abstract: A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes. The device consists of three layers: first layer contains an array of microscale wells (500 mum diameter and 150 mum depth) that help to immobilize the islets while exposed to flow and maximize the exposed surface area of the islets; the second layer contains a circular perifusion chamber (3 mm deep, 7 mm diameter); and the third layer contains an inlet-mixing channel that fans out before injection into the perifusion chamber (2 mm in width, 19 mm in length, and 500 mum in height) for optimizing the mixing efficiency prior to entering the perifusion chamber. The creation of various glucose gradients including a linear, bell shape, and square shapes also can be created in the microfluidic perifusion network and is demonstrated.
authors.affiliation: Department of Surgery.
authors.forename: Adeola F
authors.initials: AF
authors.lastname: Adewola
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Tricia
authors.initials: T
authors.lastname: Harvat
authors.forename: David T
authors.initials: DT
authors.lastname: Eddington
authors.forename: Dongyoung
authors.initials: D
authors.lastname: Lee
authors.forename: Jose
authors.initials: J
authors.lastname: Oberholzer
mesh_terms: D000818:Animals; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D007328:Insulin