title: Effects of unfocused extracorporeal shock wave therapy on healing of wounds of the distal portion of the forelimb in horses.
abstract: OBJECTIVE
  To determine effects of extracorporeal shock wave therapy (ESWT) on healing of wounds in the distal portion of the forelimb in horses.
  
  
  METHODS
  6 horses.
  
  
  METHODS
  Five 6.25-cm2 superficial wounds were created over both third metacarpi of 6 horses. Forelimbs were randomly assigned to treatment (ESWT and bandage) or control (bandage only) groups. In treated limbs, each wound was treated with 625 shock wave pulses from an unfocused electrohydraulic shock wave generator. In control limbs, each wound received sham treatment. Wound appearance was recorded weekly as inflamed or healthy and scored for the amount of protruding granulation tissue. Standardized digital photographs were used to determine the area of neoepithelialization and absolute wound area. Biopsy was performed on 1 wound on each limb every week for 6 weeks to evaluate epithelialization, fibroplasia, neovascularization, and inflammation. Immunohistochemical staining for A smooth muscle actin was used to label myofibroblasts.
  
  
  RESULTS
  Control wounds were 1.9 times as likely to appear inflamed, compared with treated wounds. Control wounds had significantly higher scores for exuberant granulation tissue. Treatment did not affect wound size or area of neoepithelialization. No significant difference was found for any of the histologic or immunohistochemical variables between groups.
  
  
  CONCLUSIONS
  Treatment with ESWT did not accelerate healing of equine distal limb wounds, but treated wounds had less exuberant granulation tissue and appeared healthier than controls. Therefore, ESWT may be useful to prevent exuberant granulation tissue formation and chronic inflammation of such wounds, but further studies are necessary before recommending ESWT for clinical application.
authors.affiliation: Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada. silveira@uoguelph.ca
authors.forename: Andressa
authors.initials: A
authors.lastname: Silveira
authors.forename: Judith B
authors.initials: JB
authors.lastname: Koenig
authors.forename: Luis G
authors.initials: LG
authors.lastname: Arroyo
authors.forename: Donald
authors.initials: D
authors.lastname: Trout
authors.forename: Noël M M
authors.initials: NM
authors.lastname: Moens
authors.forename: Jonathan
authors.initials: J
authors.lastname: LaMarre
authors.forename: Andrew
authors.initials: A
authors.lastname: Brooks
mesh_terms: D000818:Animals; D001458:Bandages; D005121:Extremities / Q000293:injuries*; D006097:Granulation Tissue; D019220:High-Energy Shock Waves / Q000627:therapeutic use*; D006736:Horses / Q000293:injuries*; D014945:Wound Healing / Q000528:radiation effects*; D014947:Wounds and Injuries / Q000662:veterinary*