Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus.
Brucella abortus is the intracellular bacterium that causes bovine brucellosis and a chronic human disease known as undulant fever. Interferon (IFN)-gamma plays critical roles in defending against intracellular bacterial infection. In this experiment, we demonstrated the difference in IFN-gamma production between the splenocytes of mice inoculated with outer membrane proteins (OMPs) of B. abortus and whole live bacteria. Our results showed that the OMP-inoculated group showed more IFN-gamma production than did the bacteria-infected group, suggesting that OMPs are candidates for the induction of immune response.
Cha
Seung-Bin
SB
Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Rayamajhi
Nabin
N
Kang
Mi-Lan
ML
Lee
Won-Jung
WJ
Shin
Min-Kyoung
MK
Yoo
Han-Sang
HS
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit; D007371:Interferon-gamma