title: Effect of topical application of Brazilian propolis on scratching behavior induced by compound 48/80 in mice.
abstract: OBJECTIVE
  We investigated the effect of topical application of Brazilian propolis on scratching behavior induced by compound 48/80 in mice.
  
  
  RESULTS
  Propolis inhibited compound 48/80-induced scratching behavior when applied immediately after treatment with propolis at a dose of 3 mg/site. Dibucaine 0.3 mg/site also significantly inhibited compound 48/80-induced scratching behavior immediately after application. On the other hand, propolis inhibited compound 48/80-induced scratching behavior even 15, 30 and 60 min after application; however, dibucaine showed no significant inhibition of compound 48/80-induced scratching behavior 15, 30 and 60 min after application. In addition, propolis had no effect on increased vascular permeability just after application, but the drug had a significant effect 15, 30 and 60 min after application. On the contrary, histamine-induced scratching behavior was inhibited significantly by propolis just after application. On the other hand, propolis significantly inhibited histamine release from rat mast cells induced by compound 48/80 at a concentration of more than 10 microg/ml.
  
  
  CONCLUSIONS
  From these results, it can be concluded that inhibition of scratching behavior induced by topical application occurred by both its local anesthetic and systemic action through inhibition of histamine release.
authors.affiliation: Department of Medicinal Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Shinmei
authors.forename: Yoto
authors.initials: Y
authors.lastname: Kagawa
authors.forename: Haruna
authors.initials: H
authors.lastname: Yano
authors.forename: Maria Alejandra
authors.initials: MA
authors.lastname: Hossen
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kamei
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000982:Antipruritics / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects*; D001938:Brazil; D002199:Capillary Permeability / Q000187:drug effects / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D011429:Propolis / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D011537:Pruritus / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology; D012867:Skin / Q000098:blood supply / Q000187:drug effects / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D000982:Antipruritics; D003189:p-Methoxy-N-methylphenethylamine; D011429:Propolis