title: Evaluation of a new experimental kit for the extraction of DNA from bones and teeth using a non-powder method.
abstract: An experimental DNA extraction kit (new kit) was recently developed to extract DNA from degraded skeletal remains without the need for powdering the samples. We compared the utility of the new kit with the conventional phenol/chloroform method using real-time quantitative PCR and multiplex STR analysis. The new kit yielded large amounts of DNA from a compact bone fragment compared with the conventional phenol/chloroform method. We were able to extract sufficient DNA for STR analysis from 75% (3 of 4) and 60% (3 of 5) of the un-powdered tooth and bone samples, respectively, using the new kit. We were able to perform mini-STR analysis of the remaining samples using DNA extracted with the new kit. Furthermore, we successfully performed mitochondrial DNA sequencing of every sample. The new kit simplifies the DNA extraction procedure as it does not require powdering samples. Decreasing the number of procedural steps in DNA extraction will be beneficial in controlling DNA contamination in laboratories. Our results suggest that the new kit may be used for the simple, simultaneous extraction of DNA from multiple samples.
authors.affiliation: First Department of Forensic Science, National Research Institute of Police Science, Chiba 277-0882, Japan. tetsushik@nrips.go.jp
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Kitayama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Koji
authors.initials: K
authors.lastname: Fujii
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakahara
authors.forename: Natsuko
authors.initials: N
authors.lastname: Mizuno
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kasai
authors.forename: Noriko
authors.initials: N
authors.lastname: Yurino
authors.forename: Takahide
authors.initials: T
authors.lastname: Yokoi
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Fukuma
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takahito
authors.initials: T
authors.lastname: Oki
authors.forename: Hideki
authors.initials: H
authors.lastname: Asamura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Fukushima
mesh_terms: D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology*; D004247:DNA / Q000302:isolation & purification*; D003648:Decalcification Technique; D018732:Forensic Anthropology / Q000379:methods*; D053803:Forensic Genetics; D006801:Humans; D017422:Sequence Analysis, DNA; D014070:Tooth / Q000378:metabolism* / Q000473:pathology*
chemical_list: D004247:DNA