title: Proteomics approaches to understand protein phosphorylation in pathway modulation.
abstract: Signaling pathways in all organisms consist of series of phosphorylation and dephosphorylation events that define directionality and allow different levels of feedback-regulation. Mass spectrometry-based proteomic analyses in recent years have led to a proteome-wide identification of thousands of phosphorylation sites in various plant species. Given this magnitude of mostly qualitative information about protein phosphorylation, discovery of specific phosphoproteins with regulatory functions represents a major challenge. In future large-scale experiments, combinations of data-driven modeling strategies based on quantitative data, targeted kinase-substrate screens, and verification in biochemical and genetic experiments are required to specifically spot phosphorylation sites with specific roles in signaling pathway modulation.
authors.affiliation: Max Planck Institut für Molekulare Pflanzenpyhsiologie, Am Mühlenberg 1, 14476 Golm, Germany. wschulze@mpimpgolm.mpg.de
authors.forename: Waltraud X
authors.initials: WX
authors.lastname: Schulze
mesh_terms: D013058:Mass Spectrometry; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D020543:Proteome