title: Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.
abstract: GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Jinek
authors.forename: Marc R
authors.initials: MR
authors.lastname: Fabian
authors.forename: Scott M
authors.initials: SM
authors.lastname: Coyle
authors.forename: Nahum
authors.initials: N
authors.lastname: Sonenberg
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Doudna
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D018360:Crystallography, X-Ray; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D039122:Poly(A)-Binding Protein I / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020836:Protein Structure, Quaternary; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D039122:Poly(A)-Binding Protein I; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C540399:TNRC6C protein, human