title: Chemical composition and antimycobacterial activity of the essential oil from Anemia tomentosa var. anthriscifolia.
abstract: The essential oil from Anemia tomentosa var. anthriscifolia showed in vitro activity against Mycobacterium tuberculosis (MIC 100 microg/ml) and therefore was characterized by gas chromatography (GC) and by gas chromatography coupled with mass spectrometry (GC-MS). The major constituents of this essential oil were triquinane sesquiterpenes: silphiperfol-6-ene (14.7%), (-)-epi-presilphiperfolan-1-ol (30.6%), presilphiperfol-7-ene (3.9%), cameroonan-7 alpha-ol (4.4%), prenopsan-8-ol (1.9%) and presilphiperfolan-8-ol (8.3%), suggesting the existence of different chemotypes for this species. The essential oil was fractionated by column chromatography and its major constituent and fractions were assayed against Mycobacterium tuberculosis and M. smegmatis. (-)-epi-Presilphiperfolan-1-ol exhibited an MIC of 120 microg/ml against M. tuberculosis H37Rv.
authors.affiliation: Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, CCS, Bloco H, Ilha do Fundão, 21941-590, Rio de Janeiro, Brazil.
authors.forename: Shaft Corrêa
authors.initials: SC
authors.lastname: Pinto
authors.forename: Gilda Guimarães
authors.initials: GG
authors.lastname: Leitão
authors.forename: Danilo Ribeiro
authors.initials: DR
authors.lastname: de Oliveira
authors.forename: Humberto Ribeiro
authors.initials: HR
authors.lastname: Bizzo
authors.forename: Daniela Fernandes
authors.initials: DF
authors.lastname: Ramos
authors.forename: Tatiane Silveira
authors.initials: TS
authors.lastname: Coelho
authors.forename: Pedro Eduardo A
authors.initials: PE
authors.lastname: Silva
authors.forename: Maria Cristina S
authors.initials: MC
authors.lastname: Lourenço
authors.forename: Suzana Guimarães
authors.initials: SG
authors.lastname: Leitão
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry* / Q000494:pharmacology*; D001938:Brazil; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000995:Antitubercular Agents; D008055:Lipids; D009822:Oils, Volatile