title: Fluorescence probe enhanced spectrofluorimetric method for the determination of sparfloxacin in tablets and biological fluids.
abstract: Yttrium-sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buffer solution (pH = 8), and various factors influencing fluorescence were investigated. Under optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of sparfloxacin over the range 8 × 10(-7) to 1.4 × 10(-5)  mol L(-1) with a correlation coefficient of 0.9997. The detection limit (S/N = 3) was determined as 9.01 × 10(-8)  mol L(-1). The mechanism of the sensitizing effect of probe was discussed. This method has been successfully applied for the determination of sparfloxacin in pharmaceuticals, human urine and serum samples; the result obtained was satisfactory.
authors.affiliation: Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt. ak_eldidamony@yahoo.com
authors.forename: Akram M
authors.initials: AM
authors.lastname: El-Didamony
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D024841:Fluoroquinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012016:Reference Values; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D015019:Yttrium / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D013607:Tablets; D015019:Yttrium; C061363:sparfloxacin