In vitro screening of essential oil from young and mature leaves of Artemisia scoparia compared to its major constituents for free radical scavenging activity.
The present study investigated the chemical characterization, and antioxidant activity of essential oil hydrodistilled from young and mature leaves of Artemisia scoparia. GC-MS analyses revealed a monoterpenoid nature (64-67%) with 44 and 31 constituents in young and mature leaves oil, respectively. The oil from young leaf contained greater amount of oxygenated compounds. Beta-myrcene (24.13%) and p-cymene (27.06%) were the major constituents in young and mature leaves oil, respectively. A. scoparia leaf oils (25-200 microg/ml) exhibited a strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity and antioxidant activity against hydroxyl radical and hydrogen peroxide. However, the activities of major constituent monoterpenes, beta-myrcene and p-cymene, were less. In general, the DPPH radical scavenging and antioxidant activity was in the order: mature leaf oil > young leaf oil > beta-myrcene > p-cymene.
Singh
Harminder Pal
HP
Department of Environment and Vocational Studies, Panjab University, Chandigarh 160 014, India. hpsingh_01@yahoo.com
Kaur
Shalinder
S
Mittal
Sunil
S
Batish
Daizy R
DR
Kohli
Ravinder K
RK
D000375:Aging / Q000502:physiology; D019306:Artemisia / Q000737:chemistry* / Q000254:growth & development; D002734:Chlorophyll / Q000032:analysis; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D016166:Free Radical Scavengers; D010938:Plant Oils; D010940:Plant Proteins; D017392:Thiobarbituric Acid Reactive Substances; D002734:Chlorophyll; D006861:Hydrogen Peroxide