Plasma membrane electron transport in frog blood vessels.
In an attempt to see if frog blood vessels possess a plasma membrane electron transport system, the postcaval vein and aorta isolated from Rana tigrina were tested for their ability to reduce ferricyanide, methylene blue, and 2,6-dichloroindophenol. While the dyes remained unchanged, ferricyanide was reduced to ferrocyanide. This reduction was resistant to inhibition by cyanide and azide. Heptane extraction or formalin fixation of the tissues markedly reduced the capability to reduce ferricyanide. Denuded aortas retained only 30% of the activity of intact tissue. Our results indicate that the amphibian postcaval vein and aorta exhibit plasma membrane electron transport.
Rao
Rashmi P
RP
Department of Biochemistry, Kasturba Medical College, Manipal 576 104, India.
Nalini
K
K
Rao
J Prakasa
JP
D015086:2,6-Dichloroindophenol / Q000737:chemistry; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism; D001386:Azides / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism*; D003486:Cyanides / Q000737:chemistry; D004579:Electron Transport / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D010084:Oxidation-Reduction; D011898:Ranidae / Q000378:metabolism*; D014680:Veins / Q000737:chemistry / Q000378:metabolism
D001386:Azides; D003486:Cyanides; D004791:Enzyme Inhibitors; D005292:Ferricyanides; D007202:Indicators and Reagents; D015086:2,6-Dichloroindophenol; D008751:Methylene Blue