title: Sulforaphane Increases Cyclin-Dependent Kinase Inhibitor, p21 Protein in Human Oral Carcinoma Cells and Nude Mouse Animal Model to Induce G(2)/M Cell Cycle Arrest.
abstract: Previously, our group reported that sulforaphane (SFN), a naturally occurring chemopreventive agent from cruciferous vegetables, effectively inhibits the proliferation of KB and YD-10B human oral squamous carcinoma cells by causing apoptosis. In this study, treatment of 20 and 40 microM of SFN for 12 h caused a cell cycle arrest in the G(2)/M phase. Cell cycle arrest induced by SFN was associated with a significant increase in the p21 protein level and a decrease in cyclin B expression, but there was no change in the cyclin A protein level. In addition, SFN increased the p21 promoter activity significantly. Furthermore, SFN induced p21 protein expression in a nude mouse xenograft model suggesting that SFN is a potent inducer of the p21 protein in human oral squamous carcinoma cells. These findings show that SFN is a promising candidate for molecular-targeting chemotherapy against human oral squamous cell carcinoma.
authors.affiliation: Department of Oral Pathology, School of Dentistry and Institute of Oral Bioscience, Brain Korea 21 project, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Jun-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Ki
authors.initials: K
authors.lastname: Han Kwon
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Jung
authors.forename: Hye-Suk
authors.initials: HS
authors.lastname: Han
authors.forename: Jung
authors.initials: J
authors.lastname: Hyun Shim
authors.forename: Sejun
authors.initials: S
authors.lastname: Oh
authors.forename: Kyeong-Hee
authors.initials: KH
authors.lastname: Choi
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Choi
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Shin
authors.forename: Dae-Ho
authors.initials: DH
authors.lastname: Leem
authors.forename: Yunjo
authors.initials: Y
authors.lastname: Soh
authors.forename: Nam-Pyo
authors.initials: NP
authors.lastname: Cho
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho