[Regulative role of TNFalpha on STEAP4 gene in matured human adipocytes].
OBJECTIVE
Human STEAP4, a novel obesity-related gene, is associated with insulin sensitivity regulation in human adipocytes. This study aimed to explore the regulative role of TNFalpha on STEAP4 gene in matured human adipocytes.


METHODS
Human preadipocytes were cultured and differentiated into matured adipocytes in vitro. Fully differentiated adipocytes (Day 17) were treated with different concentrations of TNFalpha (0, 5, 10, 25 and 50 ng/mL) for 24 hrs. Total RNA and protein were extracted from the adipocytes. Levels of STEAP4 mRNA and protein expression were determined by real-time quantitative RT-PCR and Western blot respectively.


RESULTS
Different concentrations (5, 10, 25 and 50 ng/mL) of TNFalpha treatment for 24 hrs resulted in a significant increase in the STEAP4 mRNA expression of human matured adipocytes.The maximal effect was seen in the 50 ng/mL of TNFalpha treatment group. In parallel, STEAP4 protein synthesis in matured adipocytes increased in response to TNFalpha treatment of different concentrations (5, 10, 25 and 50 ng/mL) for 24 hrs. The maximal up-regulated effect was seen in the 25 ng/mL of TNFalpha treatment group.


CONCLUSIONS
TNFalpha can up-regulate STEAP4 mRNA expression in human matured adipocytes.
Chen
Xiao-Hui
XH
Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Zhao
Ya-Ping
YP
Zhu
Chun
C
Ji
Chen-Bo
CB
Zhang
Chun-Mei
CM
Zhu
Jin-Gai
JG
Gao
Chun-Lin
CL
Guo
Xi-Rong
XR
D017667:Adipocytes / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D008565:Membrane Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D010088:Oxidoreductases; C503310:STEAP4 protein, human