Matrix supported liquid culture and machine vision analysis of regenerated shoots of gladiolus.
Enhancement of efficiency and efficacy of plant regeneration are primary goals of micropropagation. An efficient method with rapid and improved shoot regeneration of gladiolus based upon matrix supported liquid culture technique has been demonstrated. This technique in addition ensured the quality of the regenerated shoots by effectively alleviating the phenomenon of hyperhydricity. Efficacy in plant regeneration can be brought about by the ability to sort and objectively select plants or group of plants with a desired feature. A reliable, noninvasive, machine vision-neural network based sorting method for clustering photometric variants of regenerated shoots of gladiolus has also been described.
Gupta
S Dutta
SD
Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, India.
Prasad
V S S
VS
D000064:Acclimatization; D049109:Cell Proliferation; D046508:Culture Techniques; D027621:Iridaceae / Q000254:growth & development*; D007700:Kinetics; D016571:Neural Networks, Computer; D010363:Pattern Recognition, Automated; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D012038:Regeneration; D015203:Reproducibility of Results