Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis.
Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans ( Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans.
Ha
Tae Joung
TJ
Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, 1085 Neidong, Miryang 627-803, Korea. taejoung@korea.kr
Lee
Myoung-Hee
MH
Park
Chang-Hwan
CH
Pae
Suk-Bok
SB
Shim
Kang-Bo
KB
Ko
Jong-Min
JM
Shin
Sang-Ouk
SO
Baek
In-Youl
IY
Park
Keum-Yong
KY
D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007887:Fabaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006304:Health Status; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013053:Spectrophotometry; D014675:Vegetables / Q000737:chemistry
D000872:Anthocyanins; C017185:delphinidin