title: Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry.
abstract: The use of high resolution mass spectrometry to detect signature peptides within proteolytic digests of the isolated matrix M1 protein, and whole virus digests, for both human and animal strains of influenza is shown to be able to rapidly and reliably type the virus. Conserved sequences for predicted tryptic peptides were identified through alignments of matrix M1 protein sequences across all human, avian and swine strains of the influenza virus. Peptides with unique masses, when compared with those from the in silico digestion of all influenza antigens and those proteins known to contaminate egg grown strains, were identified using the purpose built FluGest algorithm. Their frequency of occurrence within the matrix M1 protein across all type A and type B strains was established with the FluAlign algorithm. The subsequent detection of the signature peptides of matrix M1 protein within proteolytic digests of type A and type B human and avian strains has been demonstrated.
authors.affiliation: School of Molecular & Microbial Biosciences, University of Sydney, Sydney, NSW, Australia.
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Schwahn
authors.forename: Jason W H
authors.initials: JW
authors.lastname: Wong
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Downard
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000378:metabolism; D001717:Birds; D017124:Conserved Sequence; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry / Q000145:classification*; D009981:Influenza B virus / Q000737:chemistry / Q000145:classification*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000821:virology*; D010446:Peptide Fragments / Q000032:analysis / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D014357:Trypsin / Q000737:chemistry; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000956:Antigens, Viral; C545845:M1 matrix protein, influenza B virus; C488936:M1 protein, Influenza A virus; D010446:Peptide Fragments; D014763:Viral Matrix Proteins; D014357:Trypsin