CD8+ T cells mediate skin allergy to amoxicillin in a mouse model.
BACKGROUND
Delayed allergic skin reactions to drugs are common iatrogenic diseases mediated by activation of specific T cells in the skin.


METHODS
To better understand the role of T cells in these diseases, we developed a mouse model of drug allergy induced by skin sensitization to amoxicillin (amox), a penicillin antibiotic frequently involved in delayed drug allergy.


RESULTS
Whereas wild-type mice could not be sensitized to amox, CD4+ T-cell-deficient mice developed an amox-specific allergic skin response, mediated by IFN-gamma-producing CD8+ T cells. Amox-specific CD8+ T cells, induced in lymphoid organs at a high frequency during sensitization, were recruited in the skin upon challenge. CD8+ T cells were effectors of the allergic skin reaction to amox as in vivo treatment with depleting anti-CD8 mAbs abrogated the skin inflammatory reaction and as purified CD8+ T cells could adoptively transfer the allergic response to naive recipients.


CONCLUSIONS
CD8+ T cells mediate penicillin skin allergy.
Rozieres
A
A
Université Lyon 1, UFR Lyon Sud, Pierre-Bénite, France.
Vocanson
M
M
Rodet
K
K
Benetiere
J
J
Bienvenu
J
J
Berard
F
F
Hennino
A
A
Nicolas
J-F
JF
D000658:Amoxicillin / Q000009:adverse effects* / Q000276:immunology; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity / Q000209:etiology / Q000276:immunology; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000276:immunology; D012871:Skin Diseases / Q000139:chemically induced / Q000276:immunology; D012882:Skin Tests
D000658:Amoxicillin