title: Genetic elements associated with antimicrobial resistance in enteropathogenic Escherichia coli (EPEC) from Brazil.
abstract: BACKGROUND
  We recently observed an association of resistance with a certain enteropathogenic Escherichia coli (EPEC) serotypes and identified a conjugative plasmid, similar to plasmid pED208, that was conserved among archival O111:H2/NM and O119:H2 strains of diverse geographical origin. In this study, we sought to determine the prevalence and distribution of this plasmid among a collection of EPEC isolates from Brazil, as well as to study the susceptibilities of these isolates to antimicrobial agents.
  
  
  RESULTS
  Resistance was more commonly seen in typical EPEC than atypical strains. The most prevalent resistances were to ampicillin, tetracycline, streptomycin and the sulfonamides. Markers for the EPEC conjugative multiresistance plasmid, were detected in 21 (30%) of typical but only 4 (5%) of atypical strains (p = 0.001, Chi-squared test). This plasmid, previously reported from only O111 and O119 strains was found in O55 and O127 strains and was associated with the presence of class 1 integrons.
  
  
  CONCLUSIONS
  Our data suggest a limited but expanding host range for the EPEC resistance plasmid.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de São Paulo, Rua Botucatu 862, São Paulo 04023-062, Brazil. scaletsky@unifesp.br
authors.forename: Isabel C A
authors.initials: IC
authors.lastname: Scaletsky
authors.forename: Tamara B
authors.initials: TB
authors.lastname: Souza
authors.forename: Katia R S
authors.initials: KR
authors.lastname: Aranda
authors.forename: Iruka N
authors.initials: IN
authors.lastname: Okeke
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D016009:Chi-Square Distribution; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D015995:Prevalence
chemical_list: D000900:Anti-Bacterial Agents