Porous poly (L-lactic acid) scaffolds are optimal substrates for internal colonization by A6 mesoangioblasts and immunocytochemical analyses.
In this study, mouse mesoangioblasts were seeded onto bidimensional matrices within three-dimensional porous scaffolds of poly (L-lactic acid) (PLLA), in the presence or absence of a type I collagen coating. The cells were observed under a scanning electron microscope and tested for their adhesion, survival and proliferation. Immunolocalization of heat shock protein (Hsp) 70, an abundant and ubiquitous intracellular protein in these cells, was also performed in sectioned cell-containing scaffolds under a confocal fluorescence microscope to determine if in situ analysis of intracellular constituents was feasible. The data show that PLLA films allow direct cell adhesion and represent an optimal support for cell growth, and that the internal surfaces of PLLA polymeric sponges can be colonized by mesoangioblasts, which can be submitted for in situ confocal microscopic analyses for possible monitoring of timedependent expression of differentiation markers.
Carfí-Pavia
F
F
Dipartimento di Ingegneria Chimica dei Processi e dei Materiali, Viale delle Scienze 90128 Palermo, Italy.
Turturici
G
G
Geraci
F
F
Brucato
V
V
La Carrubba
V
V
Luparello
C
C
Sconzo
G
G
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D050527:Mesangial Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D024042:Collagen Type I; D018840:HSP70 Heat-Shock Proteins; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)