[Complete genome sequence analysis of Japanese encephalitis virus newly isolated in China].
OBJECTIVE
To study the complete genome sequence of Japanese encephalitis virus (JEV) strain XJ69 isolated in ZheJiang province and explore its evolution.


METHODS
Overlapping primers were designed according to the full-length genomes from GenBank. RT-PCR was used to amplify the fragments and RT-PCR products were cloned T vector, sequenced and analyzed.


RESULTS
The genome of strain XJ69 and XJP613 were 10 964 nucleotides in length with a single open reading frame encoding 3432 amino acids. Comparison of the complete genome sequences of different JEV isolates showed XJ69 and XJP613 were 83.5%-99.2% and 83.4%-99.4% nucleotide sequence homology among them respectively, which resulted in 94.8%-99.7% amino acid sequence homology. Phylogenetic analysis through PrM/C,E and full-length genome showed that the XJ69 and XJP613 strain belonged to genotype I.


CONCLUSIONS
The nucleotitede sequence and deduced amino acid sequence of XJ69 and XJP613 strain were similar to that of those of genotype I of Japanese encephalitis virus. It belonged to genotype I and were close to the isolates SH17M-07.
Xie
Rong-Hui
RH
Zhu
Han-Ping
HP
Fu
Shi-Hong
SH
Cheng
Yin-Kai
YK
Xu
Fang
F
Yao
Ping-Ping
PP
Yang
Zhang-Nv
ZN
Zhou
Xiao-Long
XL
Zhu
Zhi-Yong
ZY
D000818:Animals; D002460:Cell Line; D002681:China; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny