Protective effect of kolaviron, a biflavonoid from Garcinia kola seeds, in brain of Wistar albino rats exposed to gamma-radiation.
This study was designed to evaluate the protective effect of kolaviron (KV), a biflavonoid from Garcinia kola seeds, against gamma-radiation (5 Gy)-induced oxidative stress in brain of Wistar rats. Vitamin C (VC) served as standard antioxidant. Forty-four rats were divided into 4 groups of 11 animals each. One group was un-irradiated (normal), two groups were treated with KV and VC (250 mg/kg) for 6 weeks prior to and 8 weeks after irradiation, and fourth group was only irradiated. Rats were sacrificed 1 and 8 weeks after irradiation. Cellular alterations were monitored using changes in the levels of malondialdehyde (MDA)-an index of lipid peroxidation, superoxide dismutase (SOD), glutathione-S-transferase (GST), reduced glutathione (GSH), catalase (CAT), alanine and aspartate aminotransferases (ALT and AST), urea and creatinine. MDA levels increased significantly (p<0.05) by 90% and 151% after 1 and 8 weeks of irradiation. Furthermore, levels of GSH and antioxidant enzymes were significantly (p<0.05) decreased in gamma-irradiated animals. GSH and GST decreased by 61% and 43% after 1 week, and by 75% and 74%, after 8 weeks of exposure, respectively. gamma-Irradiation decreased SOD and CAT levels by 53% and 68%, respectively, and caused significant (p<0.05) increases in serum ALT, AST and urea after 8 weeks of exposure. Treatment with KV and VC significantly decreased the levels of MDA, ALT, AST and urea. The antioxidant indices were significantly ameliorated in KV-treated animals. These data suggest that kolaviron may protect against gamma-radiation-induced oxidative stress in brain of exposed rats.
Adaramoye
Oluwatosin Adekunle
OA
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan-20005, Nigeria. aoadaramoye@yahoo.com
D000818:Animals; D044946:Biflavonoids / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000528:radiation effects*; D005419:Flavonoids / Q000494:pharmacology*; D005720:Gamma Rays / Q000009:adverse effects*; D029763:Garcinia kola / Q000737:chemistry / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry / Q000502:physiology
D044946:Biflavonoids; D005419:Flavonoids; D010936:Plant Extracts; D011837:Radiation-Protective Agents; C045492:kolaviron