Probiotic bacteria Lactobacillus rhamnosus influences the blood profile in rainbow trout Oncorhynchus mykiss (Walbaum).
This paper reports the effect of feeding probiotic diets on blood profiles in rainbow trout. Two experiments were performed: in the first, fish of average weight 75 g were offered either a commercial feed or the same incorporated with 10(9) CFU g(-1) of lactic acid bacteria Lactobacillus rhamnosus for 30 days; in the second study performed for a similar duration, fish of average weight 126 g were offered formulated diets that either contained the same bacteria in heat-killed or freeze-dried form (nearly 10(11) CFU g(-1)), or the basal diet without the bacteria. Blood samples were collected at different times after commencement of probiotic feeding to determine the total cholesterol, triglyceride contents, the plasma alkaline phosphatase activity, plasma protein and hematocrit value. The plasma cholesterol significantly increased upon probiotic feeding in the first experiment. A significant elevation (P<0.05) of plasma cholesterol and triglyceride and alkaline phosphatase activity level was found in the freeze-dried probiotic fed groups at 20 and 30 days postfeeding. This was concomitant with the increased plasma protein and hematocrit values in FD group at 20 and 30 days. Likewise, the heat-killed probiotic fed group registered significantly high values of triglycerides, alkaline phosphatase activity, and plasma protein compared to the control diet fed groups after 20 days of feeding. Thus, alterations in the blood profiles could serve as supplementary information when examining the benefits of probiotics for fish.
Panigrahi
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A
Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, Tokyo, 108-8477, Japan. apanigrahi2k@yahoo.com
Kiron
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V
Satoh
S
S
Watanabe
T
T
D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001798:Blood Proteins / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D005526:Food, Formulated / Q000382:microbiology*; D006400:Hematocrit; D052201:Lacticaseibacillus rhamnosus; D017686:Oncorhynchus mykiss / Q000097:blood*; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D014280:Triglycerides; D002784:Cholesterol; D000469:Alkaline Phosphatase