Combined mucosal and systemic immunity following pulmonary delivery of ISCOMATRIX adjuvanted recombinant antigens.
Deep pulmonary delivery of an influenza ISCOMATRIX vaccine has previously been shown to induce a combined mucosal and systemic antibody response. To explore whether this combined response is influenced by intrinsic properties of the component antigen, we examined the efficacy of deep pulmonary delivery of ISCOMATRIX vaccines containing different recombinant antigens, specifically gB glycoprotein from cytomegalovirus and a fragment of catalase from Helicobacter pylori. Both these vaccines induced antigen-specific mucosal and systemic immunity, as well as antigen-specific proliferative cellular responses. Pulmonary immunisation with ISCOMATRIX vaccines may therefore be a generic way of inducing combined systemic and mucosal immunity.
Vujanic
Ana
A
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Melbourne, VIC 3010, Australia.
Wee
Janet L K
JL
Snibson
Kenneth J
KJ
Edwards
Stirling
S
Pearse
Martin
M
Quinn
Charles
C
Moloney
Margaret
M
Taylor
Shirley
S
Scheerlinck
Jean-Pierre Y
JP
Sutton
Philip
P
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000280:Administration, Inhalation; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002374:Catalase / Q000276:immunology; D049109:Cell Proliferation; D002784:Cholesterol / Q000008:administration & dosage*; D004338:Drug Combinations; D005260:Female; D016480:Helicobacter pylori / Q000201:enzymology; D007279:Injections, Subcutaneous; D008214:Lymphocytes / Q000276:immunology; D010743:Phospholipids / Q000008:administration & dosage*; D012503:Saponins / Q000008:administration & dosage*; D012756:Sheep; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D004338:Drug Combinations; C493786:ISCOMATRIX; D010743:Phospholipids; D012503:Saponins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D002784:Cholesterol; D002374:Catalase