title: Libanoridin inhibits the mast cell-mediated allergic inflammatory reaction.
abstract: OBJECTIVE
  Corydalis heterocarpa is a biennial herb in South Korea, with spikes of yellow flowers. It has been used for as a folk medicine to cure travail and spasm. However, studies on this herb and its secondary metabolites have rarely been reported. In the present study, we isolated secondary metabolite libanlibanoridin from Corydalis heterocarpa. We have also examined the effect of libanoridin on the inflammatory cytokines production in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore, A2318 stimulated human mast cell line, HMC-1. PMA plus A23187 significantly increased interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha production compared to media control (P < 0.05).
  
  
  RESULTS
  We report that treatment with libanlibanoridin can inhibit PMA plus A23187-induced IL-1beta, IL-6, IL-8, and TNF-alpha production in a concentration-dependent manner with IC50 of 0.002, 1.38, 1.48, and 0.36 mug/ml, respectively. Maximal inhibition rates of IL-1beta, IL-6, IL-8, and TNF-alpha production by libanlibanoridin were about 117.5%, 86.22%, 86.41%, and 90.74%, respectively. libanoridin inhibits the mRNA expression of IL-1beta, IL-6, IL-8, and TNF-alpha. libanoridin also inhibits the expression of cyclooxygenase-2.
  
  
  CONCLUSIONS
  These results indicate that libanlibanoridin may be helpful in regulating mast cell-mediated allergic inflammatory response.
authors.affiliation: Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Jae-Joong
authors.initials: JJ
authors.lastname: Kim
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Kang
authors.forename: Jae-Uk
authors.initials: JU
authors.lastname: Seo
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Na-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: No-Yil
authors.initials: NY
authors.lastname: Myung
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: You-Ah
authors.initials: YA
authors.lastname: Kim
authors.forename: Young-Wan
authors.initials: YW
authors.lastname: Seo
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Kim
authors.forename: Jin-Han
authors.initials: JH
authors.lastname: Park
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Kang
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control; D020128:Inhibitory Concentration 50; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D055670:Medicine, Korean Traditional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003374:Coumarins; D016207:Cytokines; D007476:Ionophores; C544330:libanoridin; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate