title: Downregulation of mouse intestinal Na(+)-coupled glucose transporter SGLT1 by gum arabic (Acacia Senegal).
abstract: Intestinal Na(+)-coupled glucose transporter SGLT1 determines the rate of glucose transport, which in turn influences glucose-induced insulin release and development of obesity. The present study explored effects of Gum Arabic (GA), a dietary polysaccharide from dried exudates of Acacia Senegal, on intestinal glucose transport and body weight in wild-type C57Bl/6 mice. Treatment with GA (100 g/l) in drinking water for four weeks did not affect intestinal SGLT1 transcript levels but decreased SGLT1 protein abundance in jejunal brush border membrane vesicles. Glucose-induced jejunal short-circuit currents revealed that GA treatment decreased electrogenic glucose transport. Drinking a 20% glucose solution for four weeks significantly increased body weight and fasting plasma glucose concentrations, effects significantly blunted by simultaneous treatment with GA. GA further significantly blunted the increase in body weight, fasting plasma glucose and fasting insulin concentrations during high fat diet. In conclusion, the present observations disclose a completely novel effect of gum arabic, i.e. its ability to decrease intestinal SGLT1 expression and activity and thus to counteract glucose-induced obesity.
authors.affiliation: Department of Physiology, University of Tuebingen, 72076 Tuebingen, Germany.
authors.forename: Omaima
authors.initials: O
authors.lastname: Nasir
authors.forename: Ferruh
authors.initials: F
authors.lastname: Artunc
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.forename: Rexhep
authors.initials: R
authors.lastname: Rexhepaj
authors.forename: Michael
authors.initials: M
authors.lastname: Föller
authors.forename: Ammar
authors.initials: A
authors.lastname: Ebrahim
authors.forename: Daniela S
authors.initials: DS
authors.lastname: Kempe
authors.forename: Raja
authors.initials: R
authors.lastname: Biswas
authors.forename: Madhuri
authors.initials: M
authors.lastname: Bhandaru
authors.forename: Michael
authors.initials: M
authors.lastname: Walter
authors.forename: Nilufar
authors.initials: N
authors.lastname: Mohebbi
authors.forename: Carsten A
authors.initials: CA
authors.lastname: Wagner
authors.forename: Amal M
authors.initials: AM
authors.lastname: Saeed
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D015536:Down-Regulation; D005947:Glucose / Q000378:metabolism; D006170:Gum Arabic / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012964:Sodium / Q000378:metabolism*; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D007328:Insulin; D051269:Sodium-Glucose Transporter 1; D006170:Gum Arabic; D012964:Sodium; D005947:Glucose