[Effect of adenovirus-mediated stromal cell-derived factor-alpha gene transfer on ventricular remodeling in rats with myocardial infarction].
OBJECTIVE
To explore the effect of adenovirus-mediated human stromal cell-derived factor-1alpha (hSDF-1alpha) on ventricular remodeling in rats with myocardial infarction.


METHODS
A recombinant adenoviral plasmid containing hSDF-1alpha cDNA was constructed using homologous recombination in bacteria and the recombinant adenovirus particles expressing hSDF-1alpha (AdV-SDF-1) were prepared. In rat models of myocardial infarction induced by left anterior descending artery occlusion, 1x10(10) PFU AdV-SDF-1 or PFU AdV-LacZ were injected at multiple sites into the infarcted myocardium 1 h after the operation, using 200 l cell-free PBS as the control. Four weeks after the injection, the cardiac function of the rats was analyzed, and the heart tissues were taken after the measurement of hemodynamics. On serial frozen sections, histological observation and morphometric measurement were carried out using a microscopic image analysis system, and the expression of hSDF-1alpha was detected by immunocytochemistry.


RESULTS
Four weeks after AdV-SDF-1 injection, the myocardium in the infracted area showed significantly higher expression rates of hSDF-1alpha. The injection resulted in a obvious reduction in the infarct size and collagen content and a marked increase in the left ventricle wall, and the rats showed improved cardiac functions.


CONCLUSIONS
SDF-1alpha can improve the cardiac structure and function in rats with myocardial infarction by inhibiting collagen synthesis and deposition in the infarcted area.
Yang
Jian-ye
JY
Institute of Clinical Medicine, Shiyan People's Hospital, Department of Physiology, Yunyang Medical College, Shiyan 442000, China. syanghb@163.com
Zhang
Ying-chun
YC
Tang
Jun-ming
JM
An
Qing-bao
QB
Guo
Ling-yun
LY
Kong
Xia
X
Huang
Yong-zhang
YZ
Zheng
Fei
F
Wang
Jia-ning
JN
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D054377:Chemokine CXCL12 / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D020257:Ventricular Remodeling / Q000187:drug effects*
D054377:Chemokine CXCL12; D011994:Recombinant Proteins