Micropropagation of Vanda teres (Roxb.) Lindle.
For high frequency clonal propagation of Vanda teres, nodal segments are cultured on VW medium supplemented with 2% sucrose, 2 mg/L Kinetin, 0.5 mg/L NAA, 2 g/L peptone, 1 g/L activated charcoal and 2.2 g/L gelrite. The cultures are incubated at 24 +/- 2 degrees C under fluorescent light 50 micromol/m(2)/s for a 16 h photoperiod per day. The PLBs (protocorm like bodies) are induced within 12 weeks of culture and are subcultured to proliferate on the fresh nutrient culture medium for 8 weeks. The clumps of the PLBs are dissected and cultured on VW medium containing 2% sucrose, 15% coconut water (CW), 2 g/L peptone, 150 mg/L L-glutamine and 1 g/L activated charcoal. The PLB sections elongate to form shoots, and new PLBs are induced from the base within 8 weeks of culture. For plantlets formation, the shoots are cultured on VW medium amended with 2% sucrose, 15% CW, 2 g/L peptone, 1 g/L activated charcoal, 50 g/L banana pulp and 1 mg/L Indole-3-butyric acid (IBA). The regenerated plants are acclimatized and cultivated in the nursery, where they bloom within 3 years.
Alam
M Firoz
MF
Department of Botany, Rajshahi University, Rajshahi, Bangladesh.
Sinha
Pinaki
P
Hakim
M Lokman
ML
D000064:Acclimatization; D049109:Cell Proliferation; D046508:Culture Techniques; D029595:Orchidaceae / Q000254:growth & development*; D017440:Photoperiod; D018520:Plant Shoots / Q000254:growth & development; D012038:Regeneration; D036226:Seedlings / Q000254:growth & development; D013997:Time Factors