title: Equine laminitis model: lamellar histopathology seven days after induction with oligofructose.
abstract: BACKGROUND
  The histopathology of laminitis during its transition from the acute to the chronic phase has not been previously documented. Studying hoof lamellar tissues 7 days after induction of laminitis may provide insight into the intractable nature of the chronic phase of the disease.
  
  
  OBJECTIVE
  To induce laminitis and investigate hoof wall lamellar tissues 7 days after dosing.
  
  
  METHODS
  Laminitis was induced using oligofructose in 6 normal Standardbred horses. The dorsal hoof lamellar tissues of these and 12 normal horses were processed and examined by light microscopy. Serial sections of a lamellar tip affected by laminitis were used to create a 3 dimensional reconstruction.
  
  
  RESULTS
  Transverse sections of dorsal hoof wall lamellae were significantly longer than normal. Many secondary epidermal lamellae were not connected to primary lamellae and existed as spherical or ovoid, discrete islands isolated in the lamellar dermis. The lamellar basement membrane was intact.
  
  
  CONCLUSIONS
  Lamellar tissue has the ability to reorganise rapidly following an episode of acute laminitis. Although histopathological evidence of ongoing acute laminitis was absent by 7 days, there was marked disruption of lamellar architecture.
  
  
  CONCLUSIONS
  The architecture and subsequent strength of the resultant lamellar interface could be greatly influenced for the better by strategies that minimise mechanical displacement during the acute phase of laminitis.
authors.affiliation: Australian Equine Laminitis Research Unit, School of Veterinary Science, Faculty of Natural Resources Agriculture and Veterinary Science, The University of Queensland, St. Lucia, Queensland 4072, Australia.
authors.forename: A W
authors.initials: AW
authors.lastname: Van Eps
authors.forename: C C
authors.initials: CC
authors.lastname: Pollitt
mesh_terms: D000818:Animals; D005260:Female; D005534:Foot Diseases / Q000139:chemically induced* / Q000473:pathology; D006724:Hoof and Claw / Q000473:pathology*; D006734:Horse Diseases / Q000139:chemically induced* / Q000473:pathology; D006736:Horses; D007249:Inflammation / Q000473:pathology / Q000662:veterinary; D008297:Male; D009844:Oligosaccharides / Q000633:toxicity*; D013997:Time Factors
chemical_list: D009844:Oligosaccharides; C120489:oligofructose