title: Temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza.
abstract: The question of which dendritic cells (DCs) respond to pulmonary antigens and cross-prime CD8(+) T cells remains controversial. We show here that influenza-specific CD8(+) T cell priming was controlled by different DCs at different times after infection. Whereas early priming was controlled by both CD103(+)CD11b(lo) and CD103(-)CD11b(hi) DCs, CD103(-)CD11b(hi) DCs dominated antigen presentation at the peak of infection. Moreover, CD103(-)CD11b(hi) DCs captured exogenous antigens in the lungs and directly cross-primed CD8(+) T cells in the draining lymph nodes without transferring antigen to CD8alpha(+) DCs. Finally, we show that CD103(-)CD11b(hi) DCs were the only DCs to express CD70 after influenza infection and that CD70 expression on CD103(-)CD11b(hi) DCs licensed them to expand CD8(+) T cell populations responding to both influenza and exogenous ovalbumin.
authors.affiliation: Department of Medicine, Division of Allergy, Immunology and Rheumatology, University of Rochester, Rochester, New York, USA.
authors.forename: André
authors.initials: A
authors.lastname: Ballesteros-Tato
authors.forename: Beatriz
authors.initials: B
authors.lastname: León
authors.forename: Frances E
authors.initials: FE
authors.lastname: Lund
authors.forename: Troy D
authors.initials: TD
authors.lastname: Randall
mesh_terms: D000818:Animals; D053282:CD27 Ligand / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D045142:Cross-Priming / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016130:Immunophenotyping; D009980:Influenza A virus / Q000276:immunology*; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000821:virology
chemical_list: D053282:CD27 Ligand; D000949:Histocompatibility Antigens Class II; D019590:Nucleocapsid Proteins