title: [Significantly increase of glycolytic flux and pyruvate productivity in Torulopsis glabrata by heterologous expression of NADH alternative oxidase].
abstract: OBJECTIVE
  This study aimed at increasing the glycolytic flux and pyruvate productivity in Torulopsis glabrata with glucose as carbon source.
  
  
  METHODS
  For this target, we introduced a mitochondrial located alternative oxidase encoded by Histoplasma capsulatum AOX1 gene into T. glabrata, and a mutant strain named as AOX with total NADH oxidase activity was 1.8-fold higher than that of the parent stain, was achieved.
  
  
  RESULTS
  The heterologous expression of NADH alternative oxidase resulted in decrease of the dry cell weight and fermentation time by 20.3% and 10.7%, but the specific rate of glucose consumption and pyruvate production increased 34.7% and 54.1% higher, respectively. The reasons for high glycolytic flux were the intracellular NADH/NAD+ ratio and ATP concentration decreased 74.7% and 52.9% respectively, the specific activity of phosphofructokinase, pyruvate kinase increased 185.0% and 28.1%.
  
  
  CONCLUSIONS
  Introduction of a novel NADH oxidation pathway by alternative oxidase can efficiently increase the rate of glucose consumption and the target metabolite productivity.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. s19820911@sian.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Qin
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Dong
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006658:Histoplasma / Q000201:enzymology*; D024101:Mitochondrial Proteins; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins; D019289:Pyruvic Acid / Q000378:metabolism*; D000099188:Alternative Oxidase
chemical_list: D005656:Fungal Proteins; D005947:Glucose; D024101:Mitochondrial Proteins; D009243:NAD; D010088:Oxidoreductases; D010940:Plant Proteins; D019289:Pyruvic Acid; D000099188:Alternative Oxidase