The enigmatic lack of glucose utilization in Streptomyces clavuligerus is due to inefficient expression of the glucose permease gene.
Streptomyces clavuligerus ATCC 27064 is unable to use glucose but has genes for a glucose permease (glcP) and a glucose kinase (glkA). Transformation of S. clavuligerus 27064 with the Streptomyces coelicolor glcP1 gene with its own promoter results in a strain able to grow on glucose. The glcP gene of S. clavuligerus encodes a 475 amino acid glucose permease with 12 transmembrane segments. GlcP is a functional protein when expressed from the S. coelicolor glcP1 promoter and complements two different glucose transport-negative Escherichia coli mutants. Transcription studies indicate that the glcP promoter is very weak and does not allow growth on glucose. These results suggest that S. clavuligerus initially contained a functional glucose permease gene, like most other Streptomyces species, and lost the expression of this gene by adaptation to glucose-poor habitats.
Pérez-Redondo
Rosario
R
Instituto de Biotecnología, INBIOTEC, Parque Científico de León, Avda. Real  °1, 24006 León, Spain.|área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
Santamarta
Irene
I
Instituto de Biotecnología, INBIOTEC, Parque Científico de León, Avda. Real  °1, 24006 León, Spain.|área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
Bovenberg
Roel
R
DSM Anti-Infectives B.V., Delft, The Netherlands.
Martín
Juan F
JF
Instituto de Biotecnología, INBIOTEC, Parque Científico de León, Avda. Real  °1, 24006 León, Spain.|área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
Liras
Paloma
P
Instituto de Biotecnología, INBIOTEC, Parque Científico de León, Avda. Real  °1, 24006 León, Spain.|área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005941:Glucokinase / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; C096446:GlkA protein, Bacteria; D005941:Glucokinase; D005947:Glucose