Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens.
We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.
van der Linden
Jan W M
JW
Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands.
Snelders
Eveline
E
Arends
Jan P
JP
Daenen
Simon M
SM
Melchers
Willem J G
WJ
Verweij
Paul E
PE
D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001230:Aspergillus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D001393:Azoles / Q000494:pharmacology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
D000935:Antifungal Agents; D001393:Azoles; D017931:DNA Primers; D004271:DNA, Fungal