Ready-to-use house dust mites atopy patch test (HDM-Diallertest), a new screening tool for detection of house dust mites allergy in children.
OBJECTIVE
to assess the accuracy and safety of a ready-to-use atopy patch test (HDM-Diallertest, DBV Technologies, Paris) in the diagnosis of sensitization to house-dust mite (HDM) allergens in children with or without atopic dermatitis.


METHODS
prospective analysis of a systematic allergic work-up was carried out in 47 children, age 57.4+42 months (mean + SD, range 7 to 176 mo), presenting with isolated or combined atopic dermatitis (AD, n = 28) or other symptoms without AD (control group, n = 19). Children were routinely tested for specific HDM-IgE [against D. pteronyssinus (DPT) and D. farinae (DF)], and skin testing based on HDM (DPT & DF) skin prick test (SPT) and ready-to-use HDM-ATP (HDM-Diallertest), with a reading at 72 hours.


RESULTS
15 children (31.9%) exhibited specific IgE against both DPT and DF, 16 children (34.04%) exhibited positive SPT against DPT and 17 (36.1%) against DF. HDM-Diallertest was positive in 15 cases (31.9%). Among these, 9 exhibited with an eczematous reaction showed an excellent correlation with both SPT and specific IgE for DPT and DF, respectively 93.3%, 97.77%, 90.47%, and 90.47%. The different diagnostic techniques of HDM sensitization neither differ between groups, nor correlated specifically with the different clinical manifestations. No side effect was observed during and after patch testing, except for a local reaction without diffusion outside the local test area.


CONCLUSIONS
The 3 diagnostic techniques exhibited a comparable level of accuracy for the diagnosis of HDM allergens sensitization. The excellent concordance of the highest class reactions of HDM-Diallertest with the other diagnostic techniques indicates a potential role as a screening tool for the detection of HDM sensitization in infancy.
Benhamou
P H
PH
Université Paris Descartes and Department of Pediatrics-Neonatology, Pediatric Gastroenterology & Nutrition Unit, Cochin-Saint Vincent de Paul Hospital, 82 Avenue Denfert-Rochereau, 75674 Paris Cedex 14, France.
Kalach
N
N
Soulaines
P
P
Donne
N
N
Dupont
C
C
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000175:diagnosis* / Q000276:immunology; D005240:Feasibility Studies; D005260:Female; D057166:High-Throughput Screening Assays; D006801:Humans; D007223:Infant; D008297:Male; D010328:Patch Tests / Q000379:methods*; D011446:Prospective Studies; D039981:Pyroglyphidae / Q000276:immunology
D039741:Antigens, Dermatophagoides