Effect of ascorbic acid on blood oxidative stress in experimental chronic arsenicosis in rodents.
Ascorbic acid is a sugar acid and an essential vital food nutrient found mainly in fruits and vegetables. The purpose of this study was to investigate the effects of ascorbic acid against arsenic induced oxidative stress in blood of rat. In rat, treatment with ascorbic acid prevented the increased serum enzymatic activity of AST, ALT, ALP, ACP and LDH. In addition, treatment with ascorbic acid prevented elevated production of LPO, PC and NO and restored the depletion of reduced SOD and CAT activities. Interestingly, ascorbic acid markedly upregulated lymphocytes relative mRNA expression of lymphocytes SOD2 gene corresponding to GAPDH, house keeping candidate gene in arsenic-treated rat, which might provide anti-oxidative activity in the blood.
Rana
Tanmoy
T
Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata 700 037, India.
Bera
Asit Kumar
AK
Das
Subhashree
S
Pan
Diganta
D
Bandyopadhyay
Subhasish
S
Bhattacharya
Debasis
D
De
Sumanta
S
Sikdar
Sourav
S
Das
Subrata Kumar
SK
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D020261:Arsenic Poisoning / Q000378:metabolism / Q000517:prevention & control*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000378:metabolism; D002908:Chronic Disease; D004195:Disease Models, Animal; D004798:Enzymes / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D001205:Ascorbic Acid; D015415:Biomarkers; D002374:Catalase; D004798:Enzymes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009569:Nitric Oxide; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2