New horizons of genomic in situ hybridization.
This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint' plant chromosomes. In addition, we describe novel applications, e.g. how GISH banding could be used for karyotyping plant chromosomes. We further discuss recent phylogenetic applications of GISH that allow a semiquantitative signal analysis and the possibility of comparing and combining this cytogenetic technique with DNA sequence-based phylogenetic trees.
Markova
M
M
Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65 Brno, Czech Republic.
Vyskot
B
B
D000818:Animals; D001483:Base Sequence; D032461:Chromosomes, Plant; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007621:Karyotyping; D010802:Phylogeny