The effect of saffron consumption on biochemical and histopathological heart indices of rats with myocardial infarction.
This study was designed to assess the effects of saffron (Crocus sativus) on rats' heart with isoproterenol-induced myocardial injury. Animals were divided randomly into four groups: vehicle-control group (CTL); ISO group, administrated with Isoproterenol 85 mg/kg s.c.; saffron group; and finally combined Saffron + ISO group. Basal and final serum levels of heart troponin I, heart tissue antioxidants and histopathological indices were assessed in all groups. Isoproterenol administration significantly increased serum level of troponin I when compared to control group (3.46 +/- 0.77 vs. 0.53 +/- 0.35 ml in ng/ml, P < 0.001) and reduced significantly the glutathione peroxidase activity of heart muscle (1.63 +/- 0.21 vs. 4.01 +/- 0.64 nmol/mg protein, P < 0.05). The grade of heart muscle damages was severe in more than 70% of ISO group animals. Saffron + ISO group showed remarkably decreased intensity of tissue destruction and significantly decreased serum levels of heart troponin I, when compared to ISO group (1.25 +/- 0.23 vs. 3.46 +/- 0.77 ng/ml, P < 0.05). The level of glutathione peroxidase activity in Saffron + ISO animals did not have significant decline compared to saffron alone. These results suggest the protective role of saffron on ischemic hearts by biochemical and histopathological findings.
Joukar
Siyavash
S
Physiology Research Center and Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Iran. sjokar@gmail.com
Najafipour
Hamid
H
Khaksari
Mohammad
M
Sepehri
Gholamreza
G
Shahrokhi
Nader
N
Dabiri
Shahriar
S
Gholamhoseinian
Ahmad
A
Hasanzadeh
Soheil
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D027622:Crocus; D005979:Glutathione Peroxidase / Q000378:metabolism; D006331:Heart Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D019210:Troponin I / Q000097:blood
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D019210:Troponin I; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase