Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV).
The new H1N1 swine-origin influenza virus (S-OIV) strain is a global health problem. The elucidation of the virus-host relationship is crucial for the control of the new infection. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza strain at very low concentrations were cloned before the emergence of S-OIV from a patient who had a broad-range H1N1 serum neutralizing activity. The two HMabs neutralized all tested H1N1 strains, including S-OIV and a swine strain with IC(50) ranging from 2 to 7 microg/ml. Data demonstrate that infection with previously circulating H1N1 strains can elicit antibodies neutralizing S-OIV. Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection.
Burioni
Roberto
R
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy. Electronic address: Burioni.roberto@hsr.it.
Canducci
Filippo
F
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Mancini
Nicasio
N
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Clementi
Nicola
N
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Sassi
Monica
M
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
De Marco
Donata
D
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Diotti
Roberta Antonia
RA
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Saita
Diego
D
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Sampaolo
Michela
M
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Sautto
Giuseppe
G
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Pianezze
Matteo
M
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
Clementi
Massimo
M
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002645:Chickens / Q000821:virology; D004306:Dose-Response Relationship, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000276:immunology / Q000821:virology; D008875:Middle Aged; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000821:virology; D016415:Sequence Alignment; D013552:Swine / Q000821:virology; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments