title: Trypanosoma cruzi-specific immune responses in subjects from endemic areas of Chagas disease of Argentina.
abstract: Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-gamma ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-gamma responses were detected in 42% of subjects evaluated (15/26 positive by conventional serology and 22/62 seronegative subjects). Using immunoblotting, T. cruzi-specific IgG reactivity was detected in all seropositive subjects and in 11% (7/61) of subjects negative by conventional serology. Measurements of T cell responses and antibodies by immunoblotting, in conjunction with conventional serology, might enhance the capability of detection of exposure to T. cruzi in endemic areas.
authors.affiliation: Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, Buenos Aires 1063, Argentina.
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Olivera
authors.forename: Maria C
authors.initials: MC
authors.lastname: Albareda
authors.forename: Maria G
authors.initials: MG
authors.lastname: Alvarez
authors.forename: Ana M
authors.initials: AM
authors.lastname: De Rissio
authors.forename: Laura E
authors.initials: LE
authors.lastname: Fichera
authors.forename: Gretchen
authors.initials: G
authors.lastname: Cooley
authors.forename: Pedro
authors.initials: P
authors.lastname: Yachelini
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Hrellac
authors.forename: Hilda
authors.initials: H
authors.lastname: Riboldi
authors.forename: Susana A
authors.initials: SA
authors.lastname: Laucella
authors.forename: Rick L
authors.initials: RL
authors.lastname: Tarleton
authors.forename: Miriam
authors.initials: M
authors.lastname: Postan
mesh_terms: D000328:Adult; D000368:Aged; D000913:Antibodies, Protozoan / Q000097:blood; D001118:Argentina; D014355:Chagas Disease / Q000276:immunology*; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007371:Interferon-gamma