Bipolar properties of red seabream (Pagrus major) transforming growth factor-beta in induction of the leucocytes migration.
TGF-beta is one of the pleiotropic cytokines and plays a pivotal role in immune regulation and orchestrating the subsequent healing response. Recombinant red seabream TGF-beta (rTGF-beta) mature peptide was expressed and purified under native conditions in vitro. Bio-assay showed that the rTGF-beta could significantly induce head kidney (HK) and peripheral blood (PB) leucocytes migration in a dose dependent manner, whereas the rTGF-beta suppressed HK and PB leucocyte migration when the leucocytes was activated by primed with lipopolysaccharide (LPS). Both enhancing and suppressing roles of rTGF-beta on the HKL and PBL chemotactic activity indicated that the fish TGF-beta shared the similar bipolar nature with mammalian TGF-beta. Furthermore, the results indicated that the activity of TGF-beta induction of leucocyte migration appears not to be an innate feature but function by regulation the chemokines activity. This is the first time we reported that fish TGF-beta has innate bipolar property in regulation of fish immune function.
Cai
Zhonghua
Z
Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, PR China. caizh@sz.tsinghua.edu.cn
Gao
Chunping
C
Li
Lin
L
Xing
Kezhi
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002465:Cell Movement / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D021541:Sea Bream / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta