The Aspergillus fumigatus P-type Golgi apparatus Ca2+/Mn2+ ATPase PmrA is involved in cation homeostasis and cell wall integrity but is not essential for pathogenesis.
The Aspergillus fumigatus DeltapmrA (Golgi apparatus Ca(2+)/Mn(2+) P-type ATPase) strain has osmotically suppressible basal growth defects and cationic tolerance associated with increased expression of calcineurin pathway genes. Despite increased beta-glucan and chitin content, it is hypersensitive to cell wall inhibitors but remains virulent, suggesting a role for PmrA in cation homeostasis and cell wall integrity.
Pinchai
Nadthanan
N
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA.
Juvvadi
Praveen Rao
PR
Fortwendel
Jarrod R
JR
Perfect
B Zachary
BZ
Rogg
Luise E
LE
Asfaw
Yohannes G
YG
Steinbach
William J
WJ
D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism*; D000077336:Caspofungin; D002412:Cations / Q000378:metabolism* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D054714:Echinocandins / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006056:Golgi Apparatus / Q000201:enzymology*; D006706:Homeostasis / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D055744:Invasive Pulmonary Aspergillosis / Q000235:genetics / Q000473:pathology; D053208:Kaplan-Meier Estimate; D055666:Lipopeptides; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D047071:beta-Glucans / Q000378:metabolism
D000617:Aminoglycosides; D000935:Antifungal Agents; D002412:Cations; D054714:Echinocandins; D005656:Fungal Proteins; D055666:Lipopeptides; D012462:Saline Solution, Hypertonic; D047071:beta-Glucans; D002686:Chitin; D013012:Sorbitol; D004533:Egtazic Acid; C011952:nikkomycin; D000252:Calcium-Transporting ATPases; D000077336:Caspofungin