The basic helix-loop-helix transcription factor MYC1 is involved in the regulation of the flavonoid biosynthesis pathway in grapevine.
Previous results indicated that in grapevine (Vitis vinifera), regulation of the flavonoid pathway genes by MYB transcription factors depends on their interaction with basic helix-loop-helix proteins (bHLHs). The present study describes the first functional characterization of a bHLH factor from grapevine named VvMYC1. This transcription factor is phylogenetically related to Arabidopsis bHLH proteins, which participate in the control of flavonoid biosynthesis and epidermal cell fate. Transient promoter and yeast two-hybrid assays demonstrated that VvMYC1 physically interacts with MYB5a, MYB5b, MYBA1/A2, and MYBPA1 to induce promoters of flavonoid pathway genes involved in anthocyanin and/or proanthocyanidin (PA) synthesis. Additionally, transient promoter assays revealed that VvMYC1 is involved in feedback regulation of its own expression. Transcript levels of VvMYC1 during berry development correlate with the synthesis of anthocyanins and PAs in skins and seeds of berries, suggesting that VvMYC1 is involved in the regulation of anthocyanins and PA synthesis in these organs. Likewise, transient expression of VvMYC1 and VvMYBA1 induces anthocyanin synthesis in grapevine suspension cells. These results suggest that VvMYC1 is part of the transcriptional cascade controlling anthocyanin and PA biosynthesis in grapevine.
Hichri
Imène
I
Institut des Sciences de la Vigne et du Vin, UMR 1287 Ecophysiologie et Génomique Fonctionnelle de la Vigne--INRA, Université de Bordeaux, 210 Chemin de Leysotte, 33882 Villenave d'Ornon, France.
Heppel
Simon C
SC
Pillet
Jérémy
J
Léon
Céline
C
Czemmel
Stefan
S
Delrot
Serge
S
Lauvergeat
Virginie
V
Bogs
Jochen
J
D000872:Anthocyanins / Q000096:biosynthesis; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis* / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D044945:Proanthocyanidins / Q000096:biosynthesis; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D027843:Vitis / Q000235:genetics / Q000378:metabolism*
D000872:Anthocyanins; D051792:Basic Helix-Loop-Helix Proteins; D005419:Flavonoids; D010940:Plant Proteins; D044945:Proanthocyanidins; C013221:proanthocyanidin