Phase behavior and coassembly of DNA and lysozyme in dilute aqueous mixtures: a model investigation of DNA-protein interactions.
Results from an experimental investigation of the phase behavior of an aqueous system of DNA from salmon testes and the protein lysozyme are presented. At very low concentrations of either or both of the macromolecular components, wormlike assemblies with a width of the order of 10 nm are formed. There are strong indications that direct interactions between the protein units are instrumental both in driving the phase separation and in controlling the morphology of the formed assemblies.
Lundberg
Dan
D
Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal. dlundberg@qui.uc.pt
Carnerup
Anna M
AM
Schillén
Karin
K
Miguel
Maria da Graça
Mda G
Lindman
Björn
B
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D014867:Water / Q000737:chemistry*
D004268:DNA-Binding Proteins; D014867:Water; D004247:DNA; D009113:Muramidase