Effects of enzymatic digestion on compressive properties of rat intervertebral discs.
Enzymatic treatments were applied to rat motion segments to establish structure-function relationships and determine mechanical parameters most sensitive to simulated remodeling and degeneration. Rat caudal and lumbar disc biomechanical behaviors were evaluated to improve knowledge of their similarities and differences due to their frequent use during in vivo models. Caudal motion segments were assigned to four groups: soaked (control), genipin treated, elastase treated, and collagenase treated. Fresh lumbar and caudal discs were also compared. The mechanical protocol involved five force-controlled loading stages: equilibration, cyclic compression-tension, quasi-static compression, frequency sweep, and creep. Crosslinking was found to have the greatest effect on IVD properties at resting stress. Elastin's role was greatest in tension and at higher force conditions, where GAG content was also a contributing factor. Collagenase treatment caused tissue compaction, which impacted mechanical properties at both high and low force conditions. Equilibration creep and cyclic compression-tension tests were the mechanical tests most sensitive to alterations in specific matrix constituents. Caudal and lumbar motion segments had many similarities but biomechanical differences suggested some distinctions in collagenous structure and water transport characteristics in addition to the geometric differences. Results provide a basis for interpreting biomechanical changes observed in animal model studies of degeneration and remodeling, and underscore the need to maintain and/or repair collagen integrity in IVD health and disease.
Barbir
Ana
A
College of Engineering and Mathematical Sciences, University of Vermont, 33 Colchester Avenue, 207 Perkins Hall, Burlington, VT 05405, USA. ana.barbir@uvm.edu
Michalek
Arthur J
AJ
Abbott
Rosalyn D
RD
Iatridis
James C
JC
D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000737:chemistry / Q000502:physiology; D017364:Collagenases; D019245:Compressive Strength; D004549:Elastin / Q000737:chemistry / Q000502:physiology; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000737:chemistry / Q000502:physiology*; D055959:Intervertebral Disc Degeneration / Q000503:physiopathology; D008954:Models, Biological; D009068:Movement; D051381:Rats; D017207:Rats, Sprague-Dawley
D003094:Collagen; D004549:Elastin; D017364:Collagenases