Evaluation of gene polymorphisms in exercise-induced oxidative stress and damage.
Many potentially significant genetic variants related to oxidative stress have been identified and performance in endurance sports is a multi-factorial phenotype. Thus, it was decided to investigate the influences of the haptoglobin (Hp), MnSOD (Val9Ala), CAT (21A/T), GPX1 (Pro198Leu), ACE, glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) genes' polymorphisms on the oxidative stress and damage suffered by human athletes (runners). Blood samples taken immediately after a race were submitted to genotyping, comet and TBARS assays, biochemical analyses of creatine kinase (CK), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). MnSOD significantly influenced results of CK and a possible association between Hp1F-1S and Hp1S-2 genotypes with a superior TBARS values was found. Higher or lower TBARS and CK values or DNA damage also depended on the interaction between Hp and ACE or GST genotypes, indicating that MnSOD and Hp polymorphisms can be determining factors in performance, at least for runners.
Akimoto
Arthur Kenji
AK
Departamento de Genética e Morfologia, Laboratório de Genética, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília/DF, Brazil.
Miranda-Vilela
Ana Luisa
AL
Alves
Penha Cristina Zaidan
PC
Pereira
Luiz Carlos da Silva
LC
Lordelo
Graciana Souza
GS
Hiragi
Cassia de Oliveira
Cde O
da Silva
Izabel Cristina Rodrigues
IC
Grisolia
Cesar Koppe
CK
Klautau-Guimarães
Maria de Nazaré
Mde N
D000293:Adolescent; D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D056352:Athletes; D002374:Catalase / Q000235:genetics; D020552:Comet Assay; D003402:Creatine Kinase / Q000097:blood; D004249:DNA Damage / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics; D006242:Haptoglobins / Q000235:genetics; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012420:Running / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics; D055815:Young Adult
D006242:Haptoglobins; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D007703:Peptidyl-Dipeptidase A