title: [The assessment of T regulatory cells in the peripheral blood of children with metabolic syndrome].
abstract: The metabolic syndrome is defined as the co-existence of risk factors for the development of cardio-vascular disease: obesity, hypertension, insulin resistance and dyslipidemia. For a few years there has been a growing interest in immune-inflammatory aspects of obesity and metabolic syndrome. According to many authors the disturbances in the number and(or) function of T regulatory cells are responsible for autoimmune diseases. It is possible that they play a role in a pathogenesis of chronic inflammation accompanying obesity. THE AIM OF THE STUDY was to determine the percentages of T regulatory cells in children with metabolic syndrome.
  
  
  METHODS
  Fourty seven children with metabolic syndrome were prospectively enrolled into the study according to the IDF criteria (central obesity and two of: hypertension, hypertriglycerydemia, low HDL, hyperglycemia/glucose intolerance/diabetes). With the use of five-colour flow cytometry the following percentages of T cells in the peripheral blood were assessed: CD4(+), CD4(+)CD25(high), CD4(+)CD25(high)FoxP3(+), CD4(+)CD25(high)CD127(low), CD4(+)CD25(high)FoxP3(+)CD127(low).
  
  
  RESULTS
  In the group of children with metabolic syndrome we noted lower percentages of CD4(+)CD25(high) cells compared to control children: 1.7 vs. 3.7% (p = 0.01). The differences in CD4(+)CD127(low) cells were not statistically significant: 15.7 vs. 17.6% (p = 0.1). We did not observe the differences between examined and control group in the percentages of CD4(+)CD25(high)CD127(low) and CD4(+)CD25(high)FoxP3(+) cells (respectively: 0.54% vs. 0.58%, 0.49 vs. 0.59%, p > 0.05).
  
  
  CONCLUSIONS
  Results of our investigation suggest the lower percentages of CD4(+)CD25(high) but not other Tregs subpopulation cells in children with metabolic syndrome. The further research concerning the role of Tregs in the pathogenesis of immunologic disturbances accompanying metabolic syndrome will continue.
authors.affiliation: Uniwersytet Medyczny w Białymstoku, II Klinika Chorób Dzieci. w.luczynski@wp.pl
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Łuczyński
authors.forename: Anna
authors.initials: A
authors.lastname: Stasiak-Barmuta
authors.forename: Barbara
authors.initials: B
authors.lastname: Głowińska-Olszewska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Szczepański
authors.forename: Mirosława
authors.initials: M
authors.lastname: Urban
authors.forename: Karolina
authors.initials: K
authors.lastname: Buczyńska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Rembińska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Tercjak
authors.forename: Przemysław
authors.initials: P
authors.lastname: Rećko
mesh_terms: D000293:Adolescent; D018791:CD4 Lymphocyte Count; D002648:Child; D006801:Humans; D024821:Metabolic Syndrome / Q000097:blood / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*