title: Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells.
abstract: Single hematopoietic cells from carp (Cyprinus carpio) kidney were seeded to each well of 96-well plates and cultured in the presence of a supporting cell layer and conditioned media (CM). The CM were obtained from bulk-cultured carp hematopoietic cells, in which T and macrophage-lineage cells rapidly proliferated as previously reported. After 2-3 weeks, colony formation was found in 0-4 wells of each plate. Three different morphological types of colonies were observed: "type I colonies", "type II colonies" and "mixed-type colonies". Type I colony cells were interpreted as composed by macrophage-lineage cells, since they expressed a specific macrophage marker, M-CSFR/csf1r gene, and most of them phagocytosed latex particles. Type II colony cells were interpreted as composed by T lineage cells, since they expressed several T cell marker genes including gata3, lck and TCRbeta, but did not engulf latex particles. Mixed-type colonies were interpreted as composed by both macrophages and T lineage cells. They expressed not only the M-CSFR gene but also a T cell marker gene, gata3, but not other T cell markers, such as lck and TCRbeta. These results indicated that the mixed-type colonies were developed from immature common progenitors of macrophage and T cell. In contrast, type I and type II colonies were developed from more mature and mono-potent progenitors of macrophage and T cell, respectively.
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katakura
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Miyuki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001681:Biological Assay; D002347:Carps / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002999:Clone Cells; D006411:Hematopoiesis, Extramedullary; D007668:Kidney / Q000502:physiology; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism