Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species.
OBJECTIVE
To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS).


METHODS
Prospective study.


METHODS
Tertiary care hospital.


METHODS
Infertile men (n=101).


METHODS
Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while group II (n=44) included men with seminal ROS levels (≥250 relative light units/sec/×10(6) sperm).


METHODS
Seminal ROS, TAC, sperm DNA fragmentation, ROS/TAC score were measured.


RESULTS
Group II had a high incidence of sperm DNA fragmentation than group I. The odds ratio of 1.25 for elevated ROS levels corresponded to >10% greater DNA fragmentation in our patients (95% confidence interval 1.01-1.53). Group II showed poor motility, a higher incidence of leukocytospermia, and higher ROS-TAC scores compared with group I. ROS was negatively correlated with sperm curvilinear velocity (r=-.24), linearity (r=-.24), and sperm motility (r=-.31). Sperm motility was correlated with %TUNEL(+ve) sperm (r=-.39).


CONCLUSIONS
An increase in seminal ROS levels by 25% was associated with a 10% increase in sperm DNA fragmentation. Sperm motility was affected by seminal ROS and sperm DNA fragmentation.
Mahfouz
Reda
R
Center for Reproductive Medicine, Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Sharma
Rakesh
R
Thiyagarajan
Aparna
A
Kale
Vaishali
V
Gupta
Sajal
S
Sabanegh
Edmund
E
Agarwal
Ashok
A
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D053938:DNA Fragmentation; D006801:Humans; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D012189:Retrospective Studies; D055101:Semen Analysis; D013094:Spermatozoa / Q000378:metabolism*
D000975:Antioxidants; D017382:Reactive Oxygen Species