Genetic transformation of carnation (Dianthus caryophylus L.).
This chapter describes a rapid and efficient protocol for explant preparation and genetic transformation of carnation. Node explants from greenhouse-grown plants and leaf explants from in vitro plants are infected with Agrobacterium tumefaciens AGL0 harboring pKT3 plasmid, consisting of GUS and NPTII genes. Explant preparation is an important factor to obtain the transformed plants. The GUS-staining area was located only on the cut end of explants and only explants with a cut end close to the connecting area between node and leaf, produced transformed shoots. The cocultivation medium is also an important factor for the successful genetic transformation of carnation node and leaf explants. High genetic transformation efficiency of node and leaf explants cocultured with Agrobacterium tumefaciens was achieved when the explants were cocultivated on a filter paper soaked with water or water and acetosyringone mixture (AS).
Nontaswatsri
Chalermsri
C
Department of Horticulture, Faculty of Agricultural Production, Maejo University, Chiangmai, Thailand.
Fukai
Seiichi
S
D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D049109:Cell Proliferation; D046508:Culture Techniques; D029749:Dianthus / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D019868:Kanamycin Kinase / Q000235:genetics; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D030821:Plants, Genetically Modified; D012038:Regeneration / Q000235:genetics; D013997:Time Factors; D014170:Transformation, Genetic
D019868:Kanamycin Kinase; D005966:Glucuronidase