[Establishment of a goat model of tibial bone hole defect suitable for repair using injectable bone materials].
OBJECTIVE
To prepare a goat model of tibial bone hole defect suitable for studies of bone defect repair using tissue-engineered injectable bone materials.


METHODS
A circular hole bone defect 1.2 cm in diameter was induced below the tibial medial plateau of the goat. X-ray, histological inspection, and image analysis were carried out to evaluate the validity of the model in simulating limb bone defect for the study of tissue-engineered injectable bone materials.


RESULTS
At 4 and 8 weeks after the operation, neither X-ray nor histological examination showed obvious bone tissues in the bone defect. Image analysis showed a area of new bone tissue formation of (8.79 - or + 3.63)% in the total defect area at 4 weeks, which increased to (15.41 - or + 4.21)% at 8 weeks.


CONCLUSIONS
The goat model of tibial bone hole defect established in this study is suitable for studying the ability of injectable bone materials for repairing limb bone defect, and offers a simple and reliable means to simulate the local condition of bone regeneration and mechanical environment of bone defect in the limbs.
Cheng
Wen-jun
WJ
Department of Orthopedics and Traumatology, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, China. medi_cwj@126.com
Jin
Dan
D
Pei
Guo-xian
GX
Guo
Gang
G
Li
Xu
X
Xiang
Da-yong
DY
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D006041:Goats; D007267:Injections; D008297:Male; D013977:Tibia / Q000293:injuries*; D013978:Tibial Fractures / Q000628:therapy*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes