[Human papillomavirus detection in clear cell carcinoma of the cervix].
OBJECTIVE
To investigate the infection of the high risk human papillomavirus (HPV) in the specimen of the clear cell carcinoma of the cervix.


METHODS
We extracted the nucleic acids in the formalin-fixed and paraffin-embedded specimens from a 37-year-old patient with clear cell carcinoma of the cervix and detected the HPV genotype with the nested PCR.


RESULTS
We identified HPV18, a high-risk genotype, in the specimens.


CONCLUSIONS
The HPV detection with the nested PCR was available for identification of the HPV genotype(s) in the paraffin-embedded specimens of clear cell carcinoma of the cervix (CCCC) with a high accuracy and sensitivity.
Guo
Yi-fan
YF
Department of Obstetrics and Gynaecology, PLA General Hospital, Beijing 100853, China.
Liu
Ai-jun
AJ
Wang
Xiao-li
XL
Wu
Xiao-zhou
XZ
Song
Lei
L
Liu
Hong-tu
HT
D018262:Adenocarcinoma, Clear Cell / Q000175:diagnosis / Q000821:virology*; D000328:Adult; D005260:Female; D005838:Genotype; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000821:virology*