Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells.
OBJECTIVE
To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering.


METHODS
New Zealand rabbits (n = 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles.


RESULTS
The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC.


CONCLUSIONS
The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.
Mazzetti
Marcelo Paulo Vaccari
MP
Department of Plastic Surgery, Lusiada University of Santos, Sao Paulo, Brazil.
Oliveira
Isis Sousa
IS
Miranda-Ferreira
Regiane
R
Fauaz
Grasiele
G
Ribeiro
Chaibe Nunes
CN
Gomes
Paulo Oliveira
PO
Pontes
Paulo
P
Ferreira
Alice Teixeira
AT
Eça
Lilian Piñero
LP
D000273:Adipose Tissue / Q000166:cytology* / Q000601:surgery; D000818:Animals; D002469:Cell Separation; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023421:Models, Animal; D011817:Rabbits