MicroRNA activity is suppressed in mouse oocytes.
MicroRNAs (miRNAs) are small endogenous RNAs that typically imperfectly base pair with 3' untranslated regions (3'UTRs) and mediate translational repression and mRNA degradation. Dicer, which generates small RNAs in the miRNA and RNA interference (RNAi) pathways, is essential for meiotic maturation of mouse oocytes. We found that 3'UTRs of transcripts upregulated in Dicer1(-/-) oocytes are not enriched in miRNA binding sites, implicating a weak impact of miRNAs on the maternal transcriptome. Therefore, we tested the ability of endogenous miRNAs to mediate RNA-like cleavage or translational repression of reporter mRNAs. In contrast to somatic cells, endogenous miRNAs in oocytes poorly repressed translation of mRNA reporters, whereas their RNAi-like activity was much less affected. Reporter mRNA carrying let-7-binding sites failed to localize to P body-like structures in oocytes. Our data suggest that miRNA function is downregulated during oocyte development, an idea supported by normal meiotic maturation of oocytes lacking Dgcr8, which is required for the miRNA but not the RNAi pathway (Suh et al. [1], this issue of Current Biology). Suppressing miRNA function during oocyte growth is likely an early event in reprogramming gene expression during the transition of a differentiated oocyte into pluripotent blastomeres of the embryo.
Ma
Jun
J
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018, USA.
Flemr
Matyas
M
Stein
Paula
P
Berninger
Philipp
P
Malik
Radek
R
Zavolan
Mihaela
M
Svoboda
Petr
P
Schultz
Richard M
RM
D020413:3' Untranslated Regions; D000818:Animals; D053487:DEAD-box RNA Helicases / Q000172:deficiency / Q000235:genetics; D004722:Endoribonucleases / Q000172:deficiency / Q000235:genetics; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D043244:Ribonuclease III
D020413:3' Untranslated Regions; C474044:Dgcr8 protein, mouse; D035683:MicroRNAs; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D004722:Endoribonucleases; C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases