Coculture with BJ fibroblast cells inhibits the adipogenesis and lipogenesis in 3T3-L1 cells.
Mouse or human fibroblasts are commonly used as feeder cells to prevent differentiation in stem or primary cell culture. In the present study, we addressed whether fibroblasts can affect the differentiation of adipocytes. We found that the differentiation of 3T3-L1 preadipocytes was strongly suppressed when the cells were cocultured with human fibroblast (BJ) cells. BrdU incorporation analysis indicated that mitotic clonal expansion, an early event required for 3T3-L1 cell adipogenesis, was not affected by BJ cells. The 3T3-L1 cell expression levels of peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer-binding protein alpha (C/EBPalpha), sterol regulatory element binding protein-1c, and Krüppel-like factor 15, but not those of C/EBPbeta or C/EBPdelta, were decreased by coculture with BJ cells. When mature 3T3-L1 adipocytes were cocultured with BJ cells, their lipid contents were significantly reduced, with decreased fatty acid synthase expression and increased phosphorylated form of acetyl-CoA carboxylase 1. Our data indicate that coculture with BJ fibroblast cells inhibits the adipogenesis of 3T3-L1 preadipocytes and decreases the lipogenesis of mature 3T3-L1 adipocytes.
Jeong
Hyun Jeong
HJ
Department of Biochemistry, Kwandong University College of Medicine, Gangneung, Gangwondo 210-701, Republic of Korea.
Park
Sahng Wook
SW
Kim
Hojeong
H
Park
Sang-Kyu
SK
Yoon
Dojun
D
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D050156:Adipogenesis; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts; D051741:Kruppel-Like Transcription Factors; D050155:Lipogenesis; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D004268:DNA-Binding Proteins; C467418:Klf15 protein, mouse; D051741:Kruppel-Like Transcription Factors; D047495:PPAR gamma; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors