Induction of indistinguishable gene expression patterns in rats by Vero cell-derived and mouse brain-derived Japanese encephalitis vaccines.
Transcriptomics is an objective index that reflects the overall condition of cells or tissues, and transcriptome technology, such as DNA microarray analysis, is now being introduced for the quality control of medical products. In this study, we applied DNA microarray analysis to evaluate the character of Japanese encephalitis (JE) vaccines. When administered into rat peritoneum, Vero cell-derived and mouse brain-derived JE vaccines induced similar gene expression patterns in liver and brain. Body weights and blood biochemical findings were also similar after administration of the two vaccines. Our results suggest that the two JE vaccines are likely to have equivalent characteristics with regard to reactivity in rats.
Momose
Haruka
H
Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Imai
Jun-ichi
J
Hamaguchi
Isao
I
Kawamura
Mika
M
Mizukami
Takuo
T
Naito
Seishiro
S
Masumi
Atsuko
A
Maeyama
Jun-ichi
J
Takizawa
Kazuya
K
Kuramitsu
Madoka
M
Nomura
Nobuo
N
Watanabe
Shinya
S
Yamaguchi
Kazunari
K
D000818:Animals; D001774:Blood Chemical Analysis; D001835:Body Weight; D001921:Brain / Q000276:immunology / Q000821:virology*; D002522:Chlorocebus aethiops; D018349:Encephalitis Viruses, Japanese / Q000254:growth & development* / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000517:prevention & control*; D020869:Gene Expression Profiling; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D014709:Vero Cells
D022321:Japanese Encephalitis Vaccines