Effect of ERK inhibitor on pulmonary metastasis of inoculated human adenoid cystic carcinoma cells in nude mice.
In this study, we detected the expression of phosphylated ERK1/2 in human salivary adenoid cystic carcinoma (SACC) by immunohistochemical method, the effect of various concentrations of PD98059 on the growth of ACC-M cells (a cell line of human adenoid cystic carcinoma of the salivary glands) by MTT assay, and the effect of PD98059 on the lung metastasis of inoculated ACC-M cells in nude mice. The results showed that the phosphylated ERK1/2 was overexpressed in SACC, the ACC-M cell line proliferation was inhibited by PD98059, and the lung metastasis level was reduced by PD98059, indicating that ERK may be a key molecule in targeted therapy of adenoid cystic carcinoma.
Hu
Ji-an
JA
College of Stomatology, Zhejiang University, Hangzhou, China.
Li
Yining
Y
Fang
Jinhua
J
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy / Q000201:enzymology* / Q000556:secondary*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000556:secondary*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D012468:Salivary Gland Neoplasms / Q000201:enzymology / Q000473:pathology*
D005419:Flavonoids; D047428:Protein Kinase Inhibitors; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one