[Experimental research of the inhibition of vascular endothelial growth factor C expression in gastric cancer by targeting RNA interference].
OBJECTIVE
To construct the plasmid expression vector pSIH1-H1-copGFP for RNA interference against vascular endothelial growth factor C (VEGF-C) and to evaluate its effect on the expression of VEGF-C mRNA in gastric cancer cells after transfection.


METHODS
Three siRNAs of genome sequence of VEGF-C gene were retrieved from GenBank and one negative chain was used as control. Four siRNAs were cloned into plasmid pSIH1-H1-copGFP,which were then transfected into gastric cancer cells (SGC7901). The expression of VEGF-C mRNA was analyzed by RT-PCR.


RESULTS
The recombinant plasmid of pSIH1-H1-copGFP specific for VEGF-C was confirmed by gene sequencing analysis. The target sequence obtained was completely consistent with the design. Transfection efficiency of the three siRNAs ranged from 60% to 70%. After transfection, the expression of VEGF-C mRNA in SGC7901 cells was significantly inhibited. Inhibition rates of VEGF-C mRNA expression were 35.4%, 33.8% and 81.5% in the three siRNA plasmid vectors, respectively.


CONCLUSIONS
The siRNA expression plasmid vector against VEGF-C mRNA is successfully constructed, and RNAi may be a useful technique to inhibit the lymphangiogenesis of gastric cancer.
Chen
Guo-Ting
GT
Department of Gastrointestinal Surgery, Shanghai East Hospital Affiliated to Tongji University, Shanghai 200120, China. guotingchen@163.com
Niu
Xian-Ping
XP
Li
Qi
Q
Ji
Sheng-Chao
SC
Han
Qing-Hui
QH
Liu
Yang-Zhou
YZ
Li
Xia
X
Zhang
Hui
H
Cai
Duan
D
D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
D034741:RNA, Small Interfering; D042582:Vascular Endothelial Growth Factor C