title: Flow injection analysis-solid phase extraction (FIA-SPE) method for preconcentration and determination of trace amounts of penicillins using methylene blue grafted polyurethane foam.
abstract: A simple, fast, and fully automated FIA-SPE method with UV detection for the preconcentration and determination of the investigated penicillins has been developed. This paper provides adequate procedure for the preconcentration and determination of the studied compounds in pharmaceuticals and milk samples. Penicillins (penicillin G, amoxicillin, and ampicillin) are extracted in a mincolumn packed with methylene blue grafted polyurethane foam (MBGPUF) material. The antibiotics are eluted by hydrochloric acid solution to the flow cell of UV-vis spectrophotometer at 230 nm. The analytes are preconcentrated on the sorbent at pH 8.0-9.5 and sample flow rate 3.0 mL/min. Elution was performed with 200 microL 0.2 mol L(-1) hydrochloric acid at 2 mL min(-1). Sample throughput is 12h(-1) at 120 s preconcentration time. High selectivity of the sorbent for the analytes was achieved at the specified pH range. The enrichment factors achieved are 14, 16, and 11 with 3 sigma detection limits of 12, 15, and 19 ng mL(-1) for penicillin G, amoxicillin and ampicillin, respectively. The method was successfully applied to the determination of these antibiotics in pharmaceutical control and contaminated milk samples with RSD<or=8.8%.
authors.affiliation: Chemistry Deparatment, Faculty of Science, Ain Shams University, Cairo, Egypt. elshahatmf@hotmail.com
authors.forename: M F
authors.initials: MF
authors.lastname: El-Shahat
authors.forename: N
authors.initials: N
authors.lastname: Burham
authors.forename: S M Abdel
authors.initials: SM
authors.lastname: Azeem
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification; D017022:Flow Injection Analysis / Q000379:methods; D008751:Methylene Blue / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis* / Q000302:isolation & purification; D011140:Polyurethanes / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D011140:Polyurethanes; C028279:polyurethane foam; D008751:Methylene Blue