title: Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis.
abstract: Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Yoo Seong
authors.initials: YS
authors.lastname: Choi
authors.forename: Tyler W
authors.initials: TW
authors.lastname: Johannes
authors.forename: Michael
authors.initials: M
authors.lastname: Simurdiak
authors.forename: Zengyi
authors.initials: Z
authors.lastname: Shao
authors.forename: Haige
authors.initials: H
authors.lastname: Lu
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002867:Chromones / Q000378:metabolism; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008957:Models, Genetic; D005810:Multigene Family; D011753:Pyrones / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002867:Chromones; D011753:Pyrones; C508401:spectinabilin; C012198:aureothin