[On the quantitative analysis of focal ischemic cerebral infarction by TTC staining].
It is known that ischemic cerebrovascular disease is causing enormous harm to human health on account of the resultant high morbidity and disability rate. In this connexion, the anticipated target is to control the size of focal ischemic cerebral infarction, which is also an important method for judgment of therapeutic efficacy. The key question is to survey the size accurately and objectively; at the same time, the quantitative analysis of focal ischemic cerebral infarction is the pivotal question affecting the experiment conclusion and the reliability level. In this paper are introduced and summarized the methods being recently and commonly used in survey and computation, and the studies made on quantitative analysis of focal ischemic cerebral infarction by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining method. Also are summarized the principles of dyeing in TTC method, the preparatory work, and the commonly used method of surveying and computation. It is the intent of this review to provide relevant data and suggestion for research workers.
Feng
Chunyan
C
Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Fan
Xiaonong
X
Zhang
Chunhong
C
Shi
Xuemin
X
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology*; D002544:Cerebral Infarction / Q000473:pathology*; D004396:Coloring Agents; D006801:Humans; D015427:Reperfusion Injury / Q000473:pathology; D013778:Tetrazolium Salts
D004396:Coloring Agents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium