Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats.
Stem cell-based therapy using bone marrow stromal cells (MSCs) has been expected to be a promising therapy for neuronal regeneration. To repair the injured spinal cord, neuronal differentiation of MSCs before transplantation has a more satisfactory effect. Recently, neuronal differentiation of neural progenitor/stem cells by an intracellular delivery of a pVHL-derived synthetic peptide (VHL peptide) has been shown. Here, we show that VHL peptide-delivered MSCs differentiated into neuron-like cells, and that engrafted VHL peptide-delivered MSCs more recovered the behaviors of the rats than that of nondelivered MSCs. Our result suggests that the use of VHL peptide-delivered MSCs would be a promising therapeutic strategy for repairing the injured spinal cord.
Yamazaki
Yoshiyuki
Y
Department of Orthopaedic Surgery, Yokohama City University School of Medicine, Yokohama, Japan.
Kanno
Hiroshi
H
Maeda
Kazuhiko
K
Yoshida
Tetsuhiko
T
Kobayashi
Nahoko
N
Kubo
Atsuhiko
A
Yamaguchi
Yu
Y
Saito
Tomoyuki
T
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009416:Nerve Regeneration; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D010452:Peptide Biosynthesis / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000494:pharmacology; D020127:Recovery of Function; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C051774:neurofilament protein H; D051794:Von Hippel-Lindau Tumor Suppressor Protein