title: Over-expression of caveolin-1 aggravate LPS-induced inflammatory response in AT-1 cells via up-regulation of cPLA2/p38 MAPK.
abstract: OBJECTIVE
  The aim of this study was to study the effect of caveolin-1 on the cytosolic phospholipase A2 (cPLA2), p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor kappaB (NF-kappaB) in mouse lung alveolar type-1 cells' (AT-1 cells) inflammatory response induced by LPS.
  
  
  METHODS
  Gene clone technique was used to over-express caveolin-1 in AT-1 cells by lentivirus vector. The level of tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), cPLA2, p38 MAPK and NF-kappaB was measured by ELISA, western blotting and EMSA.
  
  
  METHODS
  AT-1 cells were treated with LPS.
  
  
  RESULTS
  Over-expression of caveolin-1 not only increased the production of pro-inflammatory cytokine TNF-alpha and IL-6, but also enhanced the expression of the cPLA2, p38 MAPK, and NF-kappaB.
  
  
  CONCLUSIONS
  Our data demonstrated that over-expression of caveolin-1 aggravates the AT-1 injury induced by LPS, involving in modulation of the cPLA2 mediated by the cPLA2/p38 MAPK pathway.
authors.affiliation: Institute of Respiratory Disease, The Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Li
authors.forename: Yong-Juan
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Mao
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced* / Q000276:immunology; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D054510:Phospholipases A2, Cytosolic / Q000235:genetics / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D051242:Caveolin 1; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D048051:p38 Mitogen-Activated Protein Kinases; D054510:Phospholipases A2, Cytosolic