The regulator RamA influences cmytA transcription and cell morphology of Corynebacterium ammoniagenes.
RamA plays a regulatory role for acetate utilization and S-layer biosynthesis in Corynebacterium glutamicum. Looking for any additional role, the function of RamA was analyzed in Corynebacterium ammoniagenes, which is closely related to C. glutamicum. In this study, we showed that the DeltaramA mutant constructed by a markerless knockout strategy possessed increased cell surface hydrophobicity, leading to the formation of aggregated cell masses in liquid media. In addition, the mutant exhibited an elongated cell shape as observed by SEM, suggesting that cell wall-associated proteins might be influenced. Furthermore, cell surface proteome analysis revealed that the expression of cmytA gene encoding corynomycoloyl transferase required for cell wall biosynthesis was down-regulated in the mutant, supporting the regulatory role of RamA in cell wall assembly. These studies support a novel regulatory role of RamA in inducing the expression of proteins required for cell wall assembly.
Lee
Seok-Myung
SM
Department of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam, Korea.
Lee
Joo-Young
JY
Park
Kwang-Jin
KJ
Park
Jun-Sung
JS
Ha
Un-Hwan
UH
Kim
Younhee
Y
Lee
Heung-Shick
HS
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D003352:Corynebacterium / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D023061:Gene Order; D005798:Genes, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020543:Proteome / Q000032:analysis; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014166:Transferases / Q000096:biosynthesis*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D020543:Proteome; D014157:Transcription Factors; D014166:Transferases