Encapsulation of recombinant E. coli expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer-Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one.
Recombinant Escherichia coli cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl(2) and NaCl. More than 90% of the cell viability was preserved during the encapsulation process. Moreover, the initial enzyme activity was fully maintained within encapsulated cells while it halved in free cells. Both encapsulated and free cells reached the end point of the Baeyer-Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one to 4,9-dioxabicyclo[4.2.1]non-7-en-3-one at the same time (48 h). Similarly, the enantiomeric excess above 94% was identical for encapsulated and free cells.
Hucík
Martin
M
Faculty of Chemical and Food Technology SUT, Institute of Chemical and Environmental Engineering, Radlinského 9, 812 37, Bratislava, Slovakia.
Bucko
Marek
M
Gemeiner
Peter
P
Stefuca
Vladimír
V
Vikartovská
Alica
A
Mihovilovic
Marko D
MD
Rudroff
Florian
F
Iqbal
Naseem
N
Chorvát
Dusan
D
Lacík
Igor
I
D001704:Biopolymers; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism*; D002214:Capsules / Q000737:chemistry; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001704:Biopolymers; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002214:Capsules; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D010105:Oxygenases; C067215:cyclopentanone monooxygenase