title: Distribution of virulence genes related to adhesins and toxins in shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan.
abstract: Shiga toxin-producing Escherichia coli (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., eae (intimin, E. coli attaching and effacing), saa (STEC autoagglutinating adhesin), iha (irgA homologue adhesin), efa1 (E. coli factor for adherence), lpfA(O113) (long polar fimbriae), and ehaA (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic cdt (cytolethal distending toxin), and subAB (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for lpfA(O113) (99%), ehaA (95%), iha (94%), saa (91%), subAB (65%), and cdt-V (49%), respectively, while 2 were positive for eae (1.2%) and efa1 (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for ehaA (98%), iha (97%), efa1 (95%), and eae (95%), respectively, while 11, 2, 2, and 1 were positive for lpfA(O113) (18%), saa (3.3%), cdt-V (3.3%), and subAB (1.6%), respectively. Therefore, in human STEC isolates efa1 and eae whereas in cattle isolates saa, lpfA(O113), cdt-V and subAB were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, 598-8531, Japan.
authors.forename: Yuluo
authors.initials: Y
authors.lastname: Wu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hinenoya
authors.forename: Takashi
authors.initials: T
authors.lastname: Taguchi
authors.forename: Akira
authors.initials: A
authors.lastname: Nagita
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shima
authors.forename: Teizo
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: Norihiko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asakura
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D002417:Cattle; D017931:DNA Primers; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000235:genetics; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012703:Serotyping; D022621:Shiga Toxin / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D018830:Adhesins, Escherichia coli; D017931:DNA Primers; D022621:Shiga Toxin