Micropropagation of Phalaenopsis Blume.
For high frequency regeneration of Phalaenopsis, young leaf segments are cultured on gelrite-gelled (1/2) MS medium supplemented with 2% sucrose, 2.0 mg/L BA, 0.5 mg/L NAA, 10% coconut water (CW), 2 g/L peptone and 1 g/L activated charcoal. Cultures are incubated at 24 +/- 2 degrees C under fluorescence light 50 micromol/m(2)/s for 16 h photoperiod per day. The PLBs (protocorm like bodies) are induced within 12 weeks of culture and are subcultured to proliferate on the fresh nutrient culture medium for 8 weeks. The PLBs clumps are dissected and cultured on (1/2) MS medium containing 2% sucrose, 10% coconut water (CW), 2 g/L peptone, 150 mg/L L-glutamine and 1 g/L activated charcoal. The PLB sections elongate to form shoots and new PLBs are induced from the base within 8 weeks of culture. For plantlets formation the shoots are cultured on (1/2) MS medium supplemented with 2% sucrose, 10% CW, 2 g/L peptone, 1 g/L activated charcoal and 50 g/L banana pulp. The regenerated plants are acclimatized and cultivated in the nursery, where they bloom within 2 years.
Sinha
Pinaki
P
Department of Botany, Rajshahi University, Rajshahi, Bangladesh.
Alam
M Firoz
MF
Hakim
M Lokman
ML
D000064:Acclimatization; D049109:Cell Proliferation; D046508:Culture Techniques; D029595:Orchidaceae / Q000196:embryology / Q000254:growth & development*; D038081:Organogenesis; D017440:Photoperiod; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D012038:Regeneration; D013997:Time Factors