An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer.
An amperometric chloramphenicol (CAP) immunosensor was fabricated by covalently immobilizing anti-chloramphenicol acetyl transferase (anti-CAT) antibody on cadmium sulfide nanoparticles (CdS) modified-dendrimer that was bonded to the conducting polymer (poly 5, 2': 5', 2''-terthiophene-3'-carboxyl acid (poly-TTCA)) layer. The AuNPs, dendrimers, and CdS nanoparticles were deposited onto the polymer layer in order to enhance the sensitivity of the sensor probes. The particle sizes were determined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The immobilization of dendrimers, CdS, and anti-CAT were confirmed using energy disruptive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and quartz crystal microbalance (QCM) techniques. The detection of CAP was based on the competitive immuno-interaction between the free- and labeled-CAP for active sites of the anti-CAT. Hydrazine was used as the label for CAP, and it electrochemically catalyzed the reduction of H(2)O(2) at -0.35 V vs. Ag/AgCl. Under optimized conditions, the proposed immunosensor exhibited a linear range of CAP detection between 50 pg/mL and 950 pg/mL, and the detection limit was 45 pg/mL. The immunosensor was examined in real meat samples for the analysis of CAP.
Kim
Dong-Min
DM
Department of Chemistry and Institute of BioPhysio Sensor Technology, Pusan National University, Busan 609-735, South Korea.
Rahman
Md Aminur
MA
Do
Minh Hien
MH
Ban
Changill
C
Shim
Yoon-Bo
YB
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D002701:Chloramphenicol / Q000032:analysis*; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods; D050091:Dendrimers / Q000737:chemistry*; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011108:Polymers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry*
D019187:Cadmium Compounds; D050091:Dendrimers; D011108:Polymers; D013440:Sulfides; C034939:cadmium sulfide; D002701:Chloramphenicol