title: Kinetics of monofluorophosphate hydrolysis in a bacterial test plaque in situ.
abstract: Models to evaluate the anticaries potential of fluoride (F) formulations containing monofluorophosphate (MFP) should consider the release of F ion to the oral environment by its enzymatic hydrolysis. This was tested in situ, using a test plaque of a strain of Streptococcus mutans which presents high MFPase activity at pH 5.0. The test plaque was exposed to non-F or MFP (1,450 microg F/g) dentifrices and the fluid phase of the plaque was analyzed after 15, 30, 45 and 75 min. MFP concentration in the plaque fluid decreased over time after exposure to MFP dentifrice, but F ion reached 134.9 +/- 32.0 microM at 15 min and decreased significantly only at 75 min, suggesting continuous MFP hydrolysis by the test plaque.
authors.affiliation: Piracicaba Dental School, UNICAMP, Piracicaba, Brazil. litenuta@fop.unicamp.br
authors.forename: L M A
authors.initials: LM
authors.lastname: Tenuta
authors.forename: A A
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: C P M
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: G P
authors.initials: GP
authors.lastname: Moi
authors.forename: W J
authors.initials: WJ
authors.lastname: Silva
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000032:analysis / Q000378:metabolism*; D002863:Chromogenic Compounds; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000737:chemistry / Q000382:microbiology*; D003802:Dentifrices / Q000378:metabolism; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010710:Phosphates / Q000032:analysis / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D013053:Spectrophotometry; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult
chemical_list: D002327:Cariostatic Agents; D002863:Chromogenic Compounds; D003802:Dentifrices; D010710:Phosphates; C012980:fluorophosphate; D010744:Phosphoric Monoester Hydrolases; D005459:Fluorides; D002118:Calcium