title: Genomic characterization of a novel human papillomavirus (HPV-117) with a high viral load in a persisting wart.
abstract: Warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. Human papillomaviruses (HPV) are considered the causal agents for the development of such warts. We isolated the novel type HPV-117 from a persisting wart by rolling circle amplification. One hundred eighteen warts from immunocompetent patients (IC) and 49 warts from OTR were analyzed by HPV-117 E6 type-specific PCR. As inferred from a phylogenetic analysis, the new type HPV-117 belonged to alpha-PV species 2, including the most similar types HPV-10 and HPV-94. The general prevalence of HPV-117 in warts was 2% in IC (2/118), and 12% in OTR (6/49). The high viral load in dysplastic cells of a Verruca vulgaris was shown by in situ hybridization. Our results suggest an active role of the novel type in the development of cutaneous warts of OTR.
authors.affiliation: Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Köhler
authors.affiliation: Department of Biology, Systematic Botany and Mycology, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Gottschling
authors.affiliation: Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Förster
authors.affiliation: Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Röwert-Huber
authors.affiliation: Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany.
authors.forename: Eggert
authors.initials: E
authors.lastname: Stockfleth
authors.affiliation: Charité, Department of Dermatology, Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany. Electronic address: i.nindl@dkfz.de.
authors.forename: Ingo
authors.initials: I
authors.lastname: Nindl
mesh_terms: D000328:Adult; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D016867:Immunocompromised Host; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics; D019562:Viral Load; D014860:Warts / Q000821:virology*
chemical_list: D014759:Viral Envelope Proteins