title: Cannabinoid receptor 1 binding activity and quantitative analysis of Cannabis sativa L. smoke and vapor.
abstract: Cannabis sativa L. (cannabis) extracts, vapor produced by the Volcano vaporizer and smoke made from burning cannabis joints were analyzed by GC-flame ionization detecter (FID), GC-MS and HPLC. Three different medicinal cannabis varieties were investigated Bedrocan, Bedrobinol and Bediol. Cannabinoids plus other components such as terpenoids and pyrolytic by-products were identified and quantified in all samples. Cannabis vapor and smoke was tested for cannabinoid receptor 1 (CB1) binding activity and compared to pure Delta(9)-tetrahydrocannabinol (Delta(9)-THC). The top five major compounds in Bedrocan extracts were Delta(9)-THC, cannabigerol (CBG), terpinolene, myrcene, and cis-ocimene in Bedrobinol Delta(9)-THC, myrcene, CBG, cannabichromene (CBC), and camphene in Bediol cannabidiol (CBD), Delta(9)-THC, myrcene, CBC, and CBG. The major components in Bedrocan vapor (>1.0 mg/g) were Delta(9)-THC, terpinolene, myrcene, CBG, cis-ocimene and CBD in Bedrobinol Delta(9)-THC, myrcene and CBD in Bediol CBD, Delta(9)-THC, myrcene, CBC and terpinolene. The major components in Bedrocan smoke (>1.0 mg/g) were Delta(9)-THC, cannabinol (CBN), terpinolene, CBG, myrcene and cis-ocimene in Bedrobinol Delta(9)-THC, CBN and myrcene in Bediol CBD, Delta(9)-THC, CBN, myrcene, CBC and terpinolene. There was no statistically significant difference between CB1 binding of pure Delta(9)-THC compared to cannabis smoke and vapor at an equivalent concentration of Delta(9)-THC.
authors.affiliation: Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, P.O. Box 9502, 2300RA Leiden, the Netherlands.
authors.forename: Justin
authors.initials: J
authors.lastname: Fischedick
authors.forename: Frank
authors.initials: F
authors.lastname: Van Der Kooy
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D000818:Animals; D002186:Cannabinoids / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*; D002188:Cannabis / Q000737:chemistry*; D002460:Cell Line; D013759:Dronabinol / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D012906:Smoke / Q000032:analysis; D014835:Volatilization
chemical_list: D002186:Cannabinoids; D043884:Receptor, Cannabinoid, CB1; D012906:Smoke; D013759:Dronabinol