The zinc-fingers of KREPA3 are essential for the complete editing of mitochondrial mRNAs in Trypanosoma brucei.
Most mitochondrial mRNAs in trypanosomes undergo uridine insertion/deletion editing that is catalyzed by approximately 20S editosomes. The editosome component KREPA3 is essential for editosome structural integrity and its two zinc finger (ZF) motifs are essential for editing in vivo but not in vitro. KREPA3 function was further explored by examining the consequence of mutation of its N- and C-terminal ZFs (ZF1 and ZF2, respectively). Exclusively expressed myc-tagged KREPA3 with ZF2 mutation resulted in lower KREPA3 abundance and a relative increase in KREPA2 and KREL1 proteins. Detailed analysis of edited RNA products revealed the accumulation of partially edited mRNAs with less insertion editing compared to the partially edited mRNAs found in the cells with wild type KREPA3 expression. Mutation of ZF1 in TAP-tagged KREPA3 also resulted in accumulation of partially edited mRNAs that were shorter and only edited in the 3'-terminal editing region. Mutation of both ZFs essentially eliminated partially edited mRNA. The mutations did not affect gRNA abundance. These data indicate that both ZFs are essential for the progression of editing and perhaps its accuracy, which suggests that KREPA3 plays roles in the editing process via its ZFs interaction with editosome proteins and/or RNA substrates.
Guo
Xuemin
X
Seattle Biomedical Research Institute, Seattle, Washington, United States of America.
Ernst
Nancy Lewis
NL
Carnes
Jason
J
Stuart
Kenneth D
KD
D000818:Animals; D008928:Mitochondria / Q000235:genetics*; D009154:Mutation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D016335:Zinc Fingers
D015800:Protozoan Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C437999:TbMP42 protein, Trypanosoma brucei