[Research of ITK regulation on mouse spleen lymphocytes proliferation and differentiation].
OBJECTIVE
By using the RNAi method to inhibit Itk protein expression specificity, to observe lymphocytes proliferation and cytokines production, verify its function as a drug target.


METHODS
Designed siRNA aims at Itk sequence according to its sequence and solid structure, then electrotransfected into mouse spleen lymphocytes, We validated the decrease of Itk protein by Western-Blot, and detected the change of the cell proliferation by MTS and the change of inflammatory cytokines by ELISA.


RESULTS
Itk protein can be suppressed by Itk-siRNA, there were significantly reduced compared to its control group on cell proliferation as well as cytokine secretion such as IL-2, IL-4, IL-5, IFN-gamma. They all have statistical difference (P < 0.05).


CONCLUSIONS
Itk has an important immunomodulatory effect in mouse spleen lymphocytes proliferation and secretion of inflammatory cytokines.This can supply an experimental basis to regard Itk as drug target for inflammation therapy.
Xiao
Zong-Hui
ZH
He
Feng
F
Yao
Hai-Lan
HL
Han
Ji-Sheng
JS
Liu
Zhe-Wei
ZW
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D016207:Cytokines; D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase