title: Altered immunodominance hierarchies of CD8+ T cells in the spleen after infection at different sites is contingent on high virus inoculum.
abstract: Activated epitope-specific CD8+ T cells after virus infection can be organized into hierarchies (immunodominance), based on their ability to focus the response on few viral determinants. The mechanisms responsible for immunodominance can be multifactorial, with CD8+ T cells precursor frequencies recently highlighted as a key regulator. Employing the LCMV infection model, we demonstrate that the hierarchies were altered when comparing different sites of infection but only at high viral doses. These findings have significant implications when investigating immunity to viruses with different replication abilities that may override the influence of T cell precursor frequencies.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, ON, Canada.
authors.forename: Sarah
authors.initials: S
authors.lastname: Siddiqui
authors.forename: Esther
authors.initials: E
authors.lastname: Tarrab
authors.forename: Alain
authors.initials: A
authors.lastname: Lamarre
authors.forename: Sameh
authors.initials: S
authors.lastname: Basta
mesh_terms: D000818:Animals; D001117:Arenaviridae Infections / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology*
chemical_list: D016207:Cytokines