title: SDS-PAGE and two-dimensional maps in a radial gel format.
abstract: A novel method for performing 2-D map analysis is here reported, consisting in a modification of the second dimension run, which is performed not in a conventional square- or rectangular-size gel, but in a radial surface. This has the advantage of permitting resolution of closely adjacent bands, representing strings of isoforms of similar or identical mass but of closely spaced isoelectric points. When used in a mono-dimensional, SDS-PAGE format, this system allows the simultaneous running of 62 sample tracks. Examples are given of separation of plasma and urinary proteins.
authors.affiliation: Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Millioni
authors.forename: Manuela
authors.initials: M
authors.lastname: Miuzzo
authors.forename: Paolo
authors.initials: P
authors.lastname: Antonioli
authors.forename: Stefano
authors.initials: S
authors.lastname: Sbrignadello
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Iori
authors.forename: Ryan
authors.initials: R
authors.lastname: Dosselli
authors.forename: Lucia
authors.initials: L
authors.lastname: Puricelli
authors.forename: Markus
authors.initials: M
authors.lastname: Kolbe
authors.forename: Paolo
authors.initials: P
authors.lastname: Tessari
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels / Q000737:chemistry*; D006801:Humans; D007526:Isoelectric Point; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D013499:Surface Properties; D016482:Urinalysis
chemical_list: D001798:Blood Proteins; D005782:Gels; D011506:Proteins; D020543:Proteome