title: Gibberellic acid and cGMP-dependent transcriptional regulation in Arabidopsis thaliana.
abstract: An ever increasing amount of transcriptomic data and analysis tools provide novel insight into complex responses of biological systems. Given these resources we have undertaken to review aspects of transcriptional regulation in response to the plant hormone gibberellic acid (GA) and its second messenger guanosine 3',5'-cyclic monophosphate (cGMP) in Arabidopsis thaliana, both wild type and selected mutants. Evidence suggests enrichment of GA-responsive (GARE) elements in promoters of genes that are transcriptionally upregulated in response to cGMP but downregulated in a GA insensitive mutant (ga1-3). In contrast, in the genes upregulated in the mutant, no enrichment in the GARE is observed suggesting that GARE motifs are diagnostic for GA-induced and cGMP-dependent transcriptional upregulation. Further, we review how expression studies of GA-dependent transcription factors and transcriptional networks based on common promoter signatures derived from ab initio analyses can contribute to our understanding of plant responses at the systems level.
authors.affiliation: Department of Biotechnology, University of the Western Cape, Bellville, South Africa.
authors.forename: René
authors.initials: R
authors.lastname: Bastian
authors.forename: Adam
authors.initials: A
authors.lastname: Dawe
authors.forename: Stuart
authors.initials: S
authors.lastname: Meier
authors.forename: Ndiko
authors.initials: N
authors.lastname: Ludidi
authors.forename: Vladimir B
authors.initials: VB
authors.lastname: Bajic
authors.forename: Chris
authors.initials: C
authors.lastname: Gehring
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D005875:Gibberellins / Q000378:metabolism*; D009154:Mutation; D010937:Plant Growth Regulators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D014157:Transcription Factors; C007842:gibberellic acid; D006152:Cyclic GMP