Structure and biosynthesis of amphidinol 17, a hemolytic compound from Amphidinium carterae.
Amphidinol 17 (AM17; 1), a novel amphidinol, has been isolated from a Bahamas strain of Amphidinium carterae. This new congener contains the signature hairpin region and a Delta(6) polyene arm, whereas the polyol arm is distinct from those of other amphidinols. The pattern of acetate incorporation in 1 was directly determined by feeding a single labeled substrate, [2-(13)C]acetate. While the highly conserved regions within the amphidinol family of AM17 have exhibited identical occurrences of cleaved acetates to other amphidinols for which the biosynthesis has been explored, the polyol arm for AM17 displays a higher degree of nascent chain processing that shows similarities to amphidinolide biosynthesis. AM17 exhibited an EC(50) of 4.9 microM in a hemolytic assay using human red blood cells but displayed no detectable antifungal activity.
Meng
Yanhui
Y
Center for Marine Science, University of North Carolina Wilmington, Wilmington, North Carolina 28409, USA.
Van Wagoner
Ryan M
RM
Misner
Ian
I
Tomas
Carmelo
C
Wright
Jeffrey L C
JL
D000818:Animals; D004141:Dinoflagellida / Q000737:chemistry*; D006801:Humans; D007783:Lactones / Q000097:blood / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D000095722:Amphidinols
D007783:Lactones; C548921:amphidinol 17; D000095722:Amphidinols