Simultaneous determination of paracetamol, phenylephrine hydrochloride and chlorpheniramine maleate in pharmaceutical preparations using multivariate calibration 1.
Resolution of binary mixtures of paracetamol, phenylephrine hydrochloride and chlorpheniramine maleate with minimum sample pre-treatment and without analyte separation has been successfully achieved by methods of partial least squares algorithm with one dependent variable, principal component regression and hybrid linear analysis. Data of analysis were obtained from UV-vis spectra of the above compounds. The method of central composite design was used in the ranges of 1-15 mg L(-1) for both calibration and validation sets. The models refinement procedure and their validation were performed by cross-validation. Figures of merit such as selectivity, sensitivity, analytical sensitivity and limit of detection were determined for all three compounds. The procedure was successfully applied to simultaneous determination of the above compounds in pharmaceutical tablets.
Samadi-Maybodi
Abdolraouf
A
Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran. samadi@umz.ac.ir
Hassani Nejad-Darzi
Seyed Karim
SK
D000082:Acetaminophen / Q000032:analysis*; D002138:Calibration; D002744:Chlorpheniramine / Q000032:analysis*; D004583:Electrons; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010656:Phenylephrine / Q000032:analysis*; D025341:Principal Component Analysis; D012044:Regression Analysis; D015203:Reproducibility of Results; D013057:Spectrum Analysis
D004364:Pharmaceutical Preparations; D010656:Phenylephrine; D000082:Acetaminophen; D002744:Chlorpheniramine