Determination of nimodipine in human plasma by HPLC-ESI-MS and its application to a bioequivalence study.
A simple, specific, and precise liquid chromatographic-electrospray ionization mass spectrometric (LC-ESI-MS) method has been developed for determination of nimodipine concentration in human plasma. The method involves the addition of 200 microL of saturated sodium bicarbonate (NaHCO(3)) solution to plasma to improve the extraction recovery, liquid-liquid extraction of nimodipine from plasma samples with anhydrous diethyl ether, simple reversed-phase chromatography, and ESI mass spectrometric detection in negative ion selected ion monitoring mode (SIM) using target [M-] ions at m/z 417 and m/z 359 for nimodipine and nitrendipine (internal standard, IS), respectively. A complete analytical run was achieved within 3.5 min. The limit of quantification was 0.5 ng/mL. The method was validated within a linear range of 0.5-100 ng/mL. The correlation coefficient for the calibration regression line was 0.9995 or better. Intra- and inter-batch accuracy and precision were acceptable. Analyte was stable in a battery of stability studies. The method has been successfully used in a bioequivalence study.
Zhao
Ying
Y
School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
Zhai
Desheng
D
Chen
Xijing
X
Yu
Qiaoling
Q
He
Hui
H
Sun
Ya
Y
Gao
Zidong
Z
Wang
Lei
L
Wang
Huanhuan
H
Han
De'en
D
Ji
Hui
H
D002121:Calcium Channel Blockers / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D009553:Nimodipine / Q000097:blood* / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013810:Therapeutic Equivalency
D002121:Calcium Channel Blockers; D009553:Nimodipine