title: B-cell-T-cell activation and interaction in common variable immunodeficiency.
abstract: Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help.
authors.affiliation: Department of Infection and Immunity, The University of Sheffield, Sheffield, UK. nima_rezaei@farabi.tums.ac.ir
authors.forename: Nima
authors.initials: N
authors.lastname: Rezaei
authors.forename: James B
authors.initials: JB
authors.lastname: Wing
authors.forename: Asghar
authors.initials: A
authors.lastname: Aghamohammadi
authors.forename: Jennifer
authors.initials: J
authors.lastname: Carlring
authors.forename: Andrew
authors.initials: A
authors.lastname: Lees
authors.forename: Hossein
authors.initials: H
authors.lastname: Asgarian-Omran
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pourpak
authors.forename: Abdolfattah
authors.initials: A
authors.lastname: Sarrafnejad
authors.forename: Gholam A
authors.initials: GA
authors.lastname: Kardar
authors.forename: Tahereh
authors.initials: T
authors.lastname: Shahrestani
authors.forename: Farimah
authors.initials: F
authors.lastname: Masoumi
authors.forename: Ahad
authors.initials: A
authors.lastname: Zare
authors.forename: Shiva
authors.initials: S
authors.lastname: Saghafi
authors.forename: Shokouh
authors.initials: S
authors.lastname: Sarrafzadeh
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Foster
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Heath
authors.forename: Robert C
authors.initials: RC
authors.lastname: Read
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002478:Cells, Cultured; D017074:Common Variable Immunodeficiency / Q000097:blood / Q000276:immunology* / Q000473:pathology; D003911:Dextrans / Q000378:metabolism; D005260:Female; D006801:Humans; D007114:Immunization; D007072:Immunoglobulin D / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D019899:Paracrine Communication; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D003911:Dextrans; D007072:Immunoglobulin D