title: Simultaneous determination of citalopram and its metabolite in human plasma by LC-MS/MS applied to pharmacokinetic study.
abstract: A simple sensitive and robust method for simultaneous determination of citalopram and desmethylcitalopram was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS). A 200 microL aliquot of plasma sample was employed and deproteinized with methanol and desipramine was used as the internal standard. After vortex mixing and centrifugation, the supernatant was diluted with water (1:1, v/v) and then directly injected to analysis. Analytes were separated by a Zorbax XDB C(18) column with the mobile phase composed of acetonitrile and water (30:70, v/v) with 0.25% formic acid and monitored in MRM mode using a positive electrospray source with tandem mass spectrometry detection. The total run time was 3.5 min. The dynamic range was 0.2-100 ng/mL for citalopram and 0.25-50 ng/mL for desmethylcitalopram, respectively. Compared to the best existing literatures for plasma samples, the same LOQ for CIT (0.5 ng/mL) and lower LOQ for DCIT (0.25 vs 5 ng/mL) were reached, and less sample preparation steps and runtime (3.5 vs 10 min) were taken for our method. Accuracy and precision was lower than 8% and lower than 11.5% for either target. Validation results and its application to the analysis of plasma samples after oral administration of citalopram in healthy Chinese volunteers demonstrated the method was applicable to pharmacokinetic studies.
authors.affiliation: Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Rong
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Yifan
authors.initials: Y
authors.lastname: Xie
authors.forename: Congying
authors.initials: C
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yiping
authors.initials: Y
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Chen
mesh_terms: D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D015283:Citalopram / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D015283:Citalopram; C046304:desmethylcitalopram