Relation between human LPIN1, hypoxia and endoplasmic reticulum stress genes in subcutaneous and visceral adipose tissue.
BACKGROUND
LPIN1 is the phosphatidic acid phosphatase that produces 1,2-diacylglycerol, and thus it is related to the synthesis of triglycerides in the adipocyte. LPIN1 has a role in lipid synthesis and nuclear receptor coactivation, both of which may be involved in lipid homeostasis and metabolism. Among others, hypoxia and endoplasmic reticulum (ER) stress are being shown to be related to the adipose dysfunction found in human obesity.


OBJECTIVE
The aim of this study was to analyze LPIN1 gene expression in human adipose tissue in parallel with several hypoxia, angiogenic, ER stress and peroxisome proliferator-activated receptor (PPAR)-related genes in human obesity.


METHODS
Gene expression was quantified in abdominal (subcutaneous and visceral) adipose tissue from 62 subjects.


RESULTS
We have shown a marked association between LPIN1 and PPARalpha gene expression both in subcutaneous and visceral adipose tissues. Similarly, a strong interdependence with vascular endothelial growth factor (VEGF) gene expression was also described; in fact, LPIN1 and VEGF expression levels were significantly decreased with obesity to a similar extent.


CONCLUSIONS
These associations might suggest a possible role of LPIN1 in stress conditions that occur in chronic obesity and underlie insulin resistance.
Miranda
M
M
Unitat de Recerca, Hospital Universitari de Tarragona Joan XXIII, IISPV, Tarragona, Spain. mmiranda.hj23.ics@gencat.cat
Escoté
X
X
Ceperuelo-Mallafré
V
V
Megía
A
A
Caubet
E
E
Näf
S
S
Gómez
J M
JM
González-Clemente
J M
JM
Vicente
V
V
Vendrell
J
J
D017667:Adipocytes / Q000378:metabolism; D015153:Blotting, Western; D015992:Body Mass Index; D015687:Cell Hypoxia / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics / Q000502:physiology*; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D047493:PPAR alpha / Q000235:genetics* / Q000378:metabolism; D010711:Phosphatidate Phosphatase; D050151:Subcutaneous Fat / Q000378:metabolism
D009687:Nuclear Proteins; D047493:PPAR alpha; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase