Brevibacterium salitolerans sp. nov., an actinobacterium isolated from salt-lake sediment.
A novel bacterium, designated TRM 415(T), belonging to the genus Brevibacterium, was isolated from a sediment sample from a salt lake in Xinjiang province, China. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 415(T) was phylogenetically most closely related to Brevibacterium album YIM 90718(T) (98.4 % sequence similarity) and had low similarity (<95.5 %) to other species of the genus Brevibacterium; however, DNA-DNA hybridization studies between strain TRM 415(T) and B. album YIM 90718(T) showed only 41.3 % relatedness. Strain TRM 415(T) possessed meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, MK-8(H(2)) as the major menaquinone and polar lipids including phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C(17 : 0) and anteiso-C(15 : 0). The genomic DNA G+C content was 69&emsp14;mol%. Based on the evidence from this polyphasic study, strain TRM 415(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium salitolerans sp. nov. is proposed. The type strain is TRM 415(T) (=JCM 15900(T) =CCTCC AB 208328(T) =KCTC 19616(T)).
Guan
Tong-Wei
TW
Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625000, PR China.|Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang 843300, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhao
Ke
K
Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625000, PR China.
Xiao
Jing
J
Key Laboratory of Marine Biological Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian 361005, PR China.
Liu
Ying
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Xia
Zhan-Feng
ZF
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang 843300, PR China.
Zhang
Xiao-Ping
XP
Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625000, PR China.
Zhang
Li-Li
LL
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang 843300, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid