Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis.
OBJECTIVE
To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up).


METHODS
Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.


RESULTS
Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.


CONCLUSIONS
We found differences in Gene Ontology terms between the two groups. These differences could potentially be employed to establish markers of fertility success and to identify cellular processes and complex systems related with male infertility.
García-Herrero
Sandra
S
Instituto Universitario IVI Valencia, Universidad de Valencia, Valencia, Spain. sherrero@ivi.es
Garrido
Nicolás
N
Martínez-Conejero
José Antonio
JA
Remohí
José
J
Pellicer
Antonio
A
Meseguer
Marcos
M
D000328:Adult; D019295:Computational Biology; D016208:Databases, Factual; D020869:Gene Expression Profiling; D005796:Genes; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D055101:Semen Analysis / Q000379:methods; D012984:Software; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014019:Tissue Donors; D014158:Transcription, Genetic
D012333:RNA, Messenger