title: Expression profile of multiple aquaporins in human gastric carcinoma and its clinical significance.
abstract: BACKGROUND
  New evidence for involvement of aquaporins (AQPs) in cell migration and proliferation adds AQPs to an expanding list of effectors in tumor biology. But there is few report concerning the expression and role of AQPs in human gastric carcinogenesis so far. The aim of this current study was to investigate the expression profile of AQPs in human gastric carcinoma and its significance.
  
  
  METHODS
  We screened the expression profile of AQP0 approximately AQP12 in gastric adenocarcinoma tissues and corresponding normal mucosa from 89 patients with gastric cancer by reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis and immunochemical assay. The relationship between AQPs expression and clinicopathologic characteristics of patients was evaluated.
  
  
  RESULTS
  Based on RT-PCR of 13 AQPs examined, AQP1, 3, 4, 5 and 11 were expressed in human gastric cancers or normal gastric tissues, and AQP3, 4 and 5 exhibited differential expression between human gastric carcinomas and corresponding normal tissues, which was confirmed by Western blot analyses. Immunohistochemical assay showed that AQP4 protein was expressed mainly in the membrane of parietal cell and chief cell in the normal gastric mucosa, and absent in carcinoma tissues. AQP3 and AQP5 were detected remarkably stronger in the carcinoma tissues than that in normal mucosa by immunofluorescence. AQP3 expression in cases with undifferentiated tumor was more than that in cases with well-differentiated tumor. Both AQP3 and AQP5 expression were associated with lymph node metastasis and lymphovascular invasion in patients.
  
  
  CONCLUSIONS
  These findings of differential expressions of AQPs and their correlation with clinicopathologic characteristics implicated AQPs might play a role in human gastric carcinogenesis.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, PR China. shenlz@163.com
authors.forename: Lizong
authors.initials: L
authors.lastname: Shen
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yiming
authors.initials: Y
authors.lastname: Huang
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Shu
authors.forename: Maocai
authors.initials: M
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhang
authors.forename: Renhua
authors.initials: R
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Wenxi
authors.initials: W
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism; D051402:Aquaporin 5 / Q000235:genetics / Q000378:metabolism; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C496027:AQP3 protein, human; C496030:AQP4 protein, human; C496036:AQP5 protein, human; D051401:Aquaporin 4; D051402:Aquaporin 5; D020346:Aquaporins; D051400:Aquaporin 3