A TGF-beta mediated regulatory mechanism modulates the T cell immune response to rotavirus in adults but not in children.
Children with acute RV-gastroenteritis (GE) had low or undetectable levels of circulating IFN-gamma(+), IL-13(+), IL-2(+), IL-10(+) or IL-17(+) RV-T cells. IFN-gamma(+) T cells and low frequencies of IL-10(+) and IL-2(+) CD4(+) T cells were found in adults with RV-GE during acute and convalescence phases, respectively. Circulating single IFN-gamma(+)>double IFN-gamma(+)/IL-2(+)>single IL-2(+)RV-CD4(+)T cells were observed in healthy adults. In this group, frequencies of IFN-gamma(+) RV-T cells increased after removing CD25(+)cells, blocking TGF-beta with its natural inhibitor, LAP, or inhibiting TGF-betaRI signalling pathway with ALK5i. The frequencies of IFN-gamma(+) RV-T cells were also incremented in PBMC depleted of CD25(+)cells and treated with ALK5i, suggesting that TGFbeta inhibition may be independent of Treg cells. The ALK5i effect was observed in adults but not in children with RV-GE, who had normal numbers of TGF-beta+ Treg cells. Thus, a TGF-beta-mediated regulatory mechanism that modulates RV-T cells in adults is not evident in children.
Mesa
Martha C
MC
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Edificio 32, Bogotá, Colombia; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Edificio 50, Bogotá, Colombia. Electronic address: mmesa@javeriana.edu.co.
Gutiérrez
Lina
L
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Edificio 32, Bogotá, Colombia. Electronic address: linamaria11@yahoo.com.
Duarte-Rey
Carolina
C
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Edificio 32, Bogotá, Colombia. Electronic address: carolinaduarterey@gmail.com.
Angel
Juana
J
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Edificio 32, Bogotá, Colombia. Electronic address: jangel@javeriana.edu.co.
Franco
Manuel A
MA
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Edificio 32, Bogotá, Colombia. Electronic address: mafranco@javeriana.edu.co.
D000328:Adult; D000367:Age Factors; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D005260:Female; D005759:Gastroenteritis / Q000276:immunology / Q000821:virology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology / Q000502:physiology; D007223:Infant; D007371:Interferon-gamma / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology; D018655:Lymphocyte Count; D008297:Male; D012401:Rotavirus / Q000276:immunology; D012400:Rotavirus Infections / Q000276:immunology* / Q000821:virology; D013601:T-Lymphocytes / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology*
D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D007371:Interferon-gamma