The dolognawmeter: a novel instrument and assay to quantify nociception in rodent models of orofacial pain.
Rodent pain models play an important role in understanding the mechanisms of nociception and have accelerated the search for new treatment approaches for pain. Creating an objective metric for orofacial nociception in these models presents significant technical obstacles. No animal assay accurately measures pain-induced orofacial dysfunction that is directly comparable to human orofacial dysfunction. We developed and validated a high throughput, objective, operant, nociceptive animal assay, and an instrument to perform the assay termed the dolognawmeter, for evaluation of conditions known to elicit orofacial pain in humans. Using the device our assay quantifies gnawing function in the mouse. We quantified a behavioral index of nociception and demonstrated blockade of nociception in three models of orofacial pain: (1) TMJ inflammation, (2) masticatory myositis, and (3) head and neck cancer. This assay will be useful in the study of nociceptive mediators involved in the development and progression of orofacial pain conditions and it will also provide a unique tool for development and assessment of new therapeutic approaches.
Dolan
John C
JC
Department of Orofacial Sciences, University of California, San Francisco, USA.
Lam
David K
DK
Achdjian
Stacy H
SH
Schmidt
Brian L
BL
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000473:pathology; D004867:Equipment Design; D005157:Facial Pain / Q000175:diagnosis* / Q000473:pathology / Q000523:psychology*; D005620:Freund's Adjuvant; D006258:Head and Neck Neoplasms / Q000150:complications / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000175:diagnosis / Q000473:pathology; D008406:Masseter Muscle / Q000473:pathology; D008410:Masticatory Muscles / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009220:Myositis / Q000139:chemically induced / Q000175:diagnosis / Q000473:pathology; D009368:Neoplasm Transplantation; D010147:Pain Measurement / Q000295:instrumentation*; D013705:Temporomandibular Joint Disorders / Q000139:chemically induced / Q000175:diagnosis / Q000473:pathology
D005620:Freund's Adjuvant