Host factor Ebp1 inhibits rinderpest virus transcription in vivo.
ErbB3 binding protein Ebp1 has been shown to downregulate ErbB3 receptor-mediated signaling to inhibit cell proliferation. Rinderpest virus belongs to the family Paramyxoviridae and is characterized by the presence of a non-segmented negative-sense RNA genome. In this work, we show that rinderpest virus infection of Vero cells leads to the down-regulation of the host factor Ebp1, at both the mRNA and protein levels. Ebp1 protein has been shown to co-localize with viral inclusion bodies in infected cells, and it is packaged into virions, presumably through its interaction with the N protein or the N-RNA itself. Overexpression of Ebp1 inhibits viral transcription and multiplication in infected cells, suggesting that a mutual antagonism operates between host factor Ebp1 and the virus.
Gopinath
M
M
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Raju
S
S
Honda
A
A
Shaila
M S
MS
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D000818:Animals; D002522:Chlorocebus aethiops; D015536:Down-Regulation; D015870:Gene Expression; D012302:Rinderpest virus / Q000472:pathogenicity*; D014709:Vero Cells
D048868:Adaptor Proteins, Signal Transducing