Cytotype regulation in Drosophila melanogaster: synergism between telomeric and non-telomeric P elements.
The X-linked telomeric P elements TP5 and TP6 interact synergistically with non-telomeric P elements to repress hybrid dysgenesis. In this repression, the telomeric P elements exert maternal effects, which, however, are not sufficient to establish synergism with the non-telomeric P elements. Once synergism is established, the capacity to repress dysgenesis in the offspring of a cross persists for at least two generations after removing the telomeric P element from the genotype. At the molecular level, synergism between telomeric and non-telomeric P elements is correlated with effective elimination of P-element mRNA in the germ line. Maternally transmitted mutations in the genes aubergine, piwi and Suppressor of variegation 205 [Su(var)205] block the establishment of synergism between telomeric and non-telomeric P elements, and paternally transmitted mutations in piwi and Su(var)205 disrupt synergism that has already been established. These findings are discussed in terms of a model of cytotype regulation of P elements based on Piwi-interacting RNAs (piRNAs) that are amplified by cycling between sense and antisense species.
Belinco
Carina
C
Department of Biology, Normandale Community College, Bloomington, MN 55431, USA.
Diprima
Stephanie N
SN
Wolff
Ryan E
RE
Thorp
Michael W
MW
Buschette
Jared T
JT
Simmons
Michael J
MJ
D000818:Animals; D030801:Animals, Genetically Modified; D000090266:Chromobox Protein Homolog 5; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D012333:RNA, Messenger / Q000378:metabolism; D016615:Telomere / Q000235:genetics*
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D012333:RNA, Messenger; C000716867:Su(var)205 protein, Drosophila; D000090266:Chromobox Protein Homolog 5