Adsorptive properties of albumin, fibrinogen, and gamma-globulin on fluorinated diamond-like carbon films coated on PTFE.
Fluorinated diamond-like carbon (F-DLC) films were deposited on polytetrafluoroethylene (PTFE) using radio frequency (RF) plasma-enhanced chemical vapor deposition (CVD) by changing the ratio of tetrafluoromethane (CF(4)) and methane (CH(4)). To enhance the adhesion strength of the F-DLC film to the PTFE substrate, the PTFE surface was modified with a N(2) plasma pre-treatment. XPS analysis of the films showed that the C-C bond decreased with increases in the CF(4) ratio, whereas the C-F bond increased with the CF(4) ratio. The F/C ratio of the film also increased with the CF(4) ratio. The pull-out test showed that the adhesion strengths of the films (CF(4)-0-60%) were improved with the plasma pre-treatment. In the film without the plasma pre-treatment, adhesion strength increased with the CF(4) ratio. In contrast, in the case with the plasma pre-treatment, the adhesion strength of the F-DLC film decreased with the increased CF(4) ratio. Regarding the adsorption of albumin, fibrinogen, and gamma-globulin, the amount of adsorbed albumin on the film decreased with an increasing CF(4) ratio, and the amount of adsorbed fibrinogen and gamma-globulin increased with the CF(4) ratio. The CF(4)-0% DLC film showed the most adsorbed albumin and the least adsorbed fibrinogen and gamma-globulin. This indicates that the CF(4)-0% DLC film has higher anti-thrombogenicity than the F-DLC film.
Ozeki
K
K
Department of Mechanical Engineering, Ibaraki University, 4-12-1, Nakanarusawa, Hitachi, Ibaraki, 316-8511, Japan. ozeki@mx.ibaraki.ac.jp
Nagashima
I
I
Hirakuri
K K
KK
Masuzawa
T
T
D000327:Adsorption; D000418:Albumins; D002244:Carbon / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D004304:Dosage Forms; D005340:Fibrinogen; D010100:Oxygen / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011138:Polytetrafluoroethylene; D005719:gamma-Globulins
D000418:Albumins; D004304:Dosage Forms; D005719:gamma-Globulins; D002244:Carbon; D018130:Diamond; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D010100:Oxygen