[Protective effects of Angelica sinensis polysaccharide on immunological liver injury induced by concanavalin A in mice].
OBJECTIVE
To investigate the protective effects of Angelica sinensis polysaccharide on immunological liver injury induced by concanavalin A (ConA).


METHODS
Sixty NIH mice were randomly divided into 6 groups as normal group, model group, biphenyl group, Angelica sinensis polysaccharide high does group, Angelica sinensis polysaccharide middle dose group and Angelica sinensis polysaccharide low dose group. Immunological liver injury in mice was induced by ConA (20 mg/kg) by injection via tail vein, the control group received saline alone. In the treatment groups, each group was administrated via gavage daily. The control group was given equal volume of saline. Liver index, thymus index and spleen index, contents of serum ALT, AST, TB and NO in serum and TNF-alpha, IL-1beta, ITN-gamma in liver homogenate were measured.


RESULTS
Angelica sinensis polysaccharide remarkably increased the levels of liver index, thymus index and spleen index, decreased the contents ALT, AST, TB and NO levels in serum, and TNF-alpha, IL-1beta, ITN-gamma in liver homogenate (P < 0.05).


CONCLUSIONS
Angelica simesis polysaccharide haride can obviously protect immunological injury liver in mice and between the dose range of 30-120 mg/kg in a dose-dependent manner. Its mechanism may be relate to inhibiting T lymphocyte activity and ITN-gamma, TNF-alpha, IL-1beta, NO release.
Yu
Fa
F
Department of Pharmacy, the 458th Hospital of PLA, Guangzhou 510602, China.
D000410:Alanine Transaminase / Q000097:blood; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001713:Biphenyl Compounds / Q000008:administration & dosage / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D003208:Concanavalin A; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000097:blood; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D011134:Polysaccharides; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D009569:Nitric Oxide; D007371:Interferon-gamma; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase