title: Pharmacokinetics of luteolin and tetra-acetyl-luteolin assayed by HPLC in rats after oral administration.
abstract: Accurate and reproducible HPLC methods were developed and validated for the determination of concentrations of luteolin (LT) and tetra-acetyl-luteolin (TALT) in rat plasma. HPLC analyses were performed on an Agilent TC-C(18) column protected by a guard Agilent Zorbax Eclipse Plus. The mobile phase for LT was a binary mixture of acetonitrile-water (40:60, v/v) containing 0.5% phosphoric acid at a flow rate of 1.0 mL/min, and that for TALT was a binary mixture of methanol-water (70 : 30, v/v) containing 0.5% glacial acetic acid at the same flow rate. The UV detection wavelength for both analytes was set at 350 nm. The calibration curve was linear over the range of 40-1800 ng/mL, the lower limit of quantitation was 40 ng/mL and the lower limit of detection was 20 ng/mL for both LT and TALT. The intra- and inter-day precision (RSD) values for all samples were within 7.9%. The concentration-time curves of LT and TALT after oral administration (30 mg/kg) were both fitted to a two-compartment model. The pharmacokinetic characteristics of TALT were better than that of LT in the maximum plasma concentration (C(max)) and the area under the concentration-time curve (AUC).
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Sun
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Liu
authors.forename: Jiankai
authors.initials: J
authors.lastname: Xu
authors.forename: Sibo
authors.initials: S
authors.lastname: Liu
authors.forename: Bangqing
authors.initials: B
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Yu
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047311:Luteolin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047311:Luteolin