title: Diol appended quenchers for fluorescein boronic acid.
abstract: Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Förster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
authors.affiliation: School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Souad A
authors.initials: SA
authors.lastname: Elfeky
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Flower
authors.forename: Naoko
authors.initials: N
authors.lastname: Masumoto
authors.forename: François
authors.initials: F
authors.lastname: D'Hooge
authors.forename: Ludivine
authors.initials: L
authors.lastname: Labarthe
authors.forename: Wenbo
authors.initials: W
authors.lastname: Chen
authors.forename: Christophe
authors.initials: C
authors.lastname: Len
authors.forename: Tony D
authors.initials: TD
authors.lastname: James
authors.forename: John S
authors.initials: JS
authors.lastname: Fossey