Sublethal effects of the organic antifoulant Mexel(R)432 on osmoregulation and xenobiotic detoxification in the flatfish Solea senegalensis.
Mexel(R)432 is an anionic surfactant used as biocide in the cooling water system of power plants for biofouling reduction. Refrigeration waters from power plants do not usually follow, prior to their discharge to sea, any treatment aimed to eliminate biocides and this can have negative consequences on the aquatic fauna nearby. The effects of different concentrations of the antifoulant Mexel(R)432 (0.5, 1 and 2mgL(-1)) on osmoregulation (osmolality and Na(+)/K(+)-ATPase activity) and stress parameters (cortisol, glucose, and lactate) were assessed in juveniles of the flatfish Solea senegalensis. Gill histopathology and alterations due to oxidative stress (increased lipid peroxidation, LPO, levels) at branchial and hepatic levels were also considered. Other parameters tested were the antioxidant enzymes (catalase, CAT; glutathione peroxidase, GPX; and glutathione reductase, GR), xenobiotic metabolism defenses involved in detoxification (carboxylesterase, CbE; 7-ethoxyresorufin O-deethylase, EROD; and glutathione S-transferase, GST) and the neurotransmitter acetylcholinesterase (AChE) activity. Significant variations in osmoregulatory parameters, histological lesions and decreased branchial Na(+)/K(+)-ATPase activity were observed in exposed fish. Other gill biomarkers presented little or no significant variations in relation to controls. In contrast, hepatic parameters, such as CAT activity, were inhibited while EROD activity was initially elevated but after longer exposures it recovered basal values. These results suggested that under our experimental protocol exists toxic associated to Mexel(R)432 exposures.
López-Galindo
Cristina
C
Departamento de Tecnologías del Medio Ambiente, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Puerto Real, 11510 Cádiz, Spain.
Vargas-Chacoff
Luis
L
Nebot
Enrique
E
Casanueva
José F
JF
Rubio
Daniel
D
Solé
Montserrat
M
Mancera
Juan Miguel
JM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D004202:Disinfectants / Q000633:toxicity*; D005412:Flatfishes / Q000254:growth & development / Q000378:metabolism*; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D009994:Osmolar Concentration; D018384:Oxidative Stress; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013501:Surface-Active Agents / Q000627:therapeutic use*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity*
D000975:Antioxidants; D015415:Biomarkers; D004202:Disinfectants; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D000254:Sodium-Potassium-Exchanging ATPase