High diversity of extended-spectrum beta-lactamases in Escherichia coli isolates from Italian broiler flocks.
We characterized 67 Escherichia coli isolates with reduced susceptibility to cefotaxime or ceftiofur obtained from healthy broilers housed in five Italian farms. The bla(CTX-M-1), bla(CTX-M-32) and bla(SHV-12) beta-lactamase genes were identified on IncI1, IncN, or IncFIB plasmids. Considerable genetic diversity was detected among the extended-spectrum beta-lactamase (ESBL)-producing isolates, and we identified indistinguishable strains in unrelated farms and indistinguishable plasmids in genetically unrelated strains. The detection of highly mobile plasmids suggests a potential animal reservoir for beta-lactamase genes.
Bortolaia
Valeria
V
Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, 1870 Frederiksberg C, Denmark. vbo@life.ku.dk
Guardabassi
Luca
L
Trevisani
Marcello
M
Bisgaard
Magne
M
Venturi
Luciano
L
Bojesen
Anders Miki
AM
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D018550:Cephalosporin Resistance / Q000235:genetics; D002645:Chickens / Q000382:microbiology*; D004197:Disease Reservoirs / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000635:transmission; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D007558:Italy; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D029968:Escherichia coli Proteins; D001618:beta-Lactamases