title: Achromobacter xylosoxidans genomic characterization and correlation of randomly amplified polymorphic DNA profiles with relevant clinical features [corrected] of cystic fibrosis patients.
abstract: Achromobacter xylosoxidans is an emerging pathogen increasingly being isolated from respiratory samples of cystic fibrosis (CF) patients. Its role and clinical significance in lung pathogenesis have not yet been clarified. The aim of the present study was to genetically characterize A. xylosoxidans strains isolated from CF patients by use of randomly amplified polymorphic DNA (RAPD) profiles and to look for a possible correlation between RAPD profiles and the patients' clinical features, such as their spirometry values, the presence of concomitant chronic bacterial flora at the time of isolation, and the persistent or intermittent presence of A. xylosoxidans strains. A set of 106 strains of A. xylosoxidans were typed by RAPD analysis, and their profiles were analyzed by agglomerative hierarchical classification (AHC) and associated with the patient characteristics mentioned above by factorial discriminant analysis (FDA). The overall results obtained in this study showed that (i) there is a marked genetic relationship between strains isolated from the same patients at different times, (ii) characteristic RAPD profiles are associated with different predicted classes for forced expiratory volume in 1 s (FEV1%), (iii) some characteristic RAPD profiles are associated with different concomitant chronic flora (CCF) profiles, and (iv) there is a significant division of RAPD profiles into "persistent strains" and "intermittent strains" of A. xylosoxidans. These findings seem to imply that the lung habitats found in CF patients are capable of shaping and selecting the colonizing bacterial flora, as seems to be the case for the A. xylosoxidans strains studied.
authors.affiliation: Department of Public Health Sciences, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy. annarita.magni@uniroma1.it
authors.forename: Annarita
authors.initials: A
authors.lastname: Magni
authors.forename: Maria
authors.initials: M
authors.lastname: Trancassini
authors.forename: Paola
authors.initials: P
authors.lastname: Varesi
authors.forename: Valerio
authors.initials: V
authors.lastname: Iebba
authors.forename: Anna
authors.initials: A
authors.lastname: Curci
authors.forename: Claudia
authors.initials: C
authors.lastname: Pecoraro
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cimino
authors.forename: Serena
authors.initials: S
authors.lastname: Schippa
authors.forename: Serena
authors.initials: S
authors.lastname: Quattrucci
mesh_terms: D042441:Achromobacter denitrificans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques / Q000379:methods*; D002675:Child, Preschool; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013183:Sputum / Q000382:microbiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial