Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development.
Satellite cells are resident myogenic progenitors in postnatal skeletal muscle involved in muscle postnatal growth and adult regenerative capacity. Here, we identify and describe a population of muscle-resident stem cells, which are located in the interstitium, that express the cell stress mediator PW1 but do not express other markers of muscle stem cells such as Pax7. PW1(+)/Pax7(-) interstitial cells (PICs) are myogenic in vitro and efficiently contribute to skeletal muscle regeneration in vivo as well as generating satellite cells and PICs. Whereas Pax7 mutant satellite cells show robust myogenic potential, Pax7 mutant PICs are unable to participate in myogenesis and accumulate during postnatal growth. Furthermore, we found that PICs are not derived from a satellite cell lineage. Taken together, our findings uncover a new and anatomically identifiable population of muscle progenitors and define a key role for Pax7 in a non-satellite cell population during postnatal muscle growth.
Mitchell
Kathryn J
KJ
Myology Group, UMR S 787 INSERM, Université Pierre et Marie Curie Paris VI, Paris, 75634, France.
Pannérec
Alice
A
Cadot
Bruno
B
Parlakian
Ara
A
Besson
Vanessa
V
Gomes
Edgar R
ER
Marazzi
Giovanna
G
Sassoon
David A
DA
D000199:Actins / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D018952:Antigens, CD34 / Q000378:metabolism; D000950:Antigens, Ly / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D049109:Cell Proliferation; D019394:Ki-67 Antigen / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000378:metabolism; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000254:growth & development* / Q000502:physiology; D017570:MyoD Protein / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051761:Paired Box Transcription Factors / Q000235:genetics; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D012038:Regeneration / Q000502:physiology; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D000199:Actins; D018952:Antigens, CD34; D000950:Antigens, Ly; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D019394:Ki-67 Antigen; D051741:Kruppel-Like Transcription Factors; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; C497322:Pax7 protein, mouse; C097901:Peg3 protein, mouse; D011506:Proteins; D022661:RNA, Untranslated; C058992:Tagln protein, mouse; C544013:alpha-smooth muscle actin, mouse; C068686:Pax3 protein, mouse; D018995:Myosin Heavy Chains