title: Spatiotemporal characterization of phagocytic NADPH oxidase and oxidative destruction of intraphagosomal organisms in vivo using autofluorescence imaging and Raman microspectroscopy.
abstract: The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.
authors.affiliation: National Taiwan University College of Medicine and Hospital, Taipei, Taiwan.
authors.forename: Wei-Tien
authors.initials: WT
authors.lastname: Chang
authors.forename: Yi-Cyun
authors.initials: YC
authors.lastname: Yang
authors.forename: Hsueh-Han
authors.initials: HH
authors.lastname: Lu
authors.forename: I-Lin
authors.initials: IL
authors.lastname: Li
authors.forename: Ian
authors.initials: I
authors.lastname: Liau
mesh_terms: D002470:Cell Survival; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D019255:NADPH Oxidases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D019255:NADPH Oxidases