title: Quality assessment of a placental perfusion protocol.
abstract: Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3 ml h(-1) from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100 ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.
authors.affiliation: Institute of Public Health, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Line
authors.initials: L
authors.lastname: Mathiesen
authors.forename: Tina
authors.initials: T
authors.lastname: Mose
authors.forename: Thit Juul
authors.initials: TJ
authors.lastname: Mørck
authors.forename: Jeanette Kolstrup Søgaard
authors.initials: JK
authors.lastname: Nielsen
authors.forename: Leif Kofoed
authors.initials: LK
authors.lastname: Nielsen
authors.forename: Lisa Leth
authors.initials: LL
authors.lastname: Maroun
authors.forename: Morten Hanefeld
authors.initials: MH
authors.lastname: Dziegiel
authors.forename: Lise Grupe
authors.initials: LG
authors.lastname: Larsen
authors.forename: Lisbeth Ehlert
authors.initials: LE
authors.lastname: Knudsen
mesh_terms: D000826:Animal Testing Alternatives; D000983:Antipyrine / Q000493:pharmacokinetics; D004867:Equipment Design; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008431:Maternal-Fetal Exchange; D010477:Perfusion / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011786:Quality Control; D012098:Reproduction / Q000187:drug effects*; D018675:Toxicity Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards*
chemical_list: D000983:Antipyrine