Comparison of fluorescence tomographic imaging in mice with early-arriving and quasi-continuous-wave photons.
The highly diffuse nature of light propagation in biological tissue is a major challenge for obtaining high-fidelity fluorescence tomographic images. In this work we investigated the use of time-gated detection of early-arriving photons for reducing the effects of light scatter in mice relative to quasi-cw photons. When analyzing sinographic representations of the measured data, it was determined that early photons allowed a reduction in the measured FWHM of fluorescent targets by a factor of approximately 2-3, yielding a significant improvement in the tomographic image reconstruction quality.
Niedre
Mark
M
Department of Electrical and Computer Engineering, Northeastern University, Boston, Massachusetts 02115, USA.
Ntziachristos
Vasilis
V
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004058:Diffusion; D004867:Equipment Design; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D055095:Optics and Photonics; D017785:Photons; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014965:X-Rays
D015415:Biomarkers