[Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura].
OBJECTIVE
To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-gamma and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP.


METHODS
Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-gamma and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls.


RESULTS
Blood T-bet mRNA expression and IFN-gamma levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (p<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (p<0.01).


CONCLUSIONS
The high expression of T-bet and IFN-gamma and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.
Ren
Shao-Min
SM
Department of Peditrics, Affiliated Hospital of Innner Mongolia Medical College, Hohhot 010050, China. rensm22@yahoo.com.cn
Zhang
Chun-Xia
CX
Li
Wei-Cai
WC
D002648:Child; D002675:Child, Preschool; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*; D012333:RNA, Messenger / Q000097:blood; D020825:T-Box Domain Proteins / Q000235:genetics*; D018417:Th1 Cells / Q000276:immunology; D000098815:T-bet Transcription Factor
D050990:GATA3 Transcription Factor; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007371:Interferon-gamma