[Env gene sequencing and HIV-1 subtyping of infected MSM from Beijing].
OBJECTIVE
To comprehend the latest HIV-I epidemic tendency and the character of V3 loop in MSM population of Beijing. METHODS; The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-l-infected MSM in Beijing in 2007. The subtype and sequences of V3 loop was analyzed. RESULTS There are 4 subtype B strains, 5 CRF AE, 1 CRFO7BC and 1 CRF15-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common.


CONCLUSIONS
Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing.
Zhang
Hong-Hai
HH
STD & HIV/AIDS Research Laboratory, You' An Hosipital, affiliated to Capital Medical University, Beijing 100069, China.
Sun
Yu
Y
Liu
Ya-Li
YL
Wu
Ya-Song
YS
Dai
Li-Li
LL
Wu
Hao
H
Chen
De-Xi
DX
D000328:Adult; D000595:Amino Acid Sequence; D002681:China; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics*
D054299:env Gene Products, Human Immunodeficiency Virus