title: Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling.
abstract: Gadonanotubes (GNTs), which are powerful new T(1)-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml(-1)) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 x 10(9) Gd(3+) ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T(1)-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.
authors.affiliation: Breast Center, Department of Medicine and Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Amy A
authors.initials: AA
authors.lastname: Hassan
authors.forename: Bonita Tak-Yee
authors.initials: BT
authors.lastname: Chan
authors.forename: Lesa A
authors.initials: LA
authors.lastname: Tran
authors.forename: Keith B
authors.initials: KB
authors.lastname: Hartman
authors.forename: Jeyarama S
authors.initials: JS
authors.lastname: Ananta
authors.forename: Yuri
authors.initials: Y
authors.lastname: Mackeyev
authors.forename: Lingyun
authors.initials: L
authors.lastname: Hu
authors.forename: Robia G
authors.initials: RG
authors.lastname: Pautler
authors.forename: Lon J
authors.initials: LJ
authors.lastname: Wilson
authors.forename: Adrian V
authors.initials: AV
authors.lastname: Lee
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D048430:Cell Shape; D005260:Female; D005682:Gadolinium / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging / Q000379:methods; D015335:Molecular Probes / Q000378:metabolism*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D019047:Phantoms, Imaging; D012694:Serine / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D015335:Molecular Probes; D012694:Serine; D005682:Gadolinium