Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds.
A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 microM, and a linearity up to 100 microM (slope: -304 nA microM(-1); intercept: -191 nA, r(2)=0.996, n=19). Among others, it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules.
Arecchi
Alessandra
A
Dipartimento di Scienze e Tecnologie Alimentari, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy.
Scampicchio
Matteo
M
Drusch
Stephan
S
Mannino
Saverio
S
D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D059808:Polyphenols; D015203:Reproducibility of Results
D004800:Enzymes, Immobilized; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D014442:Monophenol Monooxygenase