[Resistin inhibits rat insulinoma cell RINm5F proliferation].
OBJECTIVE
Resistin was thought to link the obesity to type 2 diabetes. This study aimed to investigate the effect of resistin on insulinoma cell proliferation.


METHODS
pcDNA3.1-resistin was transfected into rat insulinoma cells RINm5F. Cell proliferation was assessed by the MTT assay. The resistin and SOCS3 mRNA levels were assessed by RT-PCR. The total Akt level and the phosphorylation status were assessed by Western blot.


RESULTS
The over-expressed resistin inhibited the RINm5F cell proliferation (p<0.05). SOCS-3 expression was up-regulated by resistin over-expression (3.2 folds over the control; p<0.05). Akt phosphorylation was down-regulated by resistin over-expression (0.6 fold over the control; p<0.05).


CONCLUSIONS
Resistin impairs the rat insulinoma cell RINm5F proliferation. This might be attributed to a down-regulation of Akt level caused by increased SOCS-3 expression.
Liu
Feng
F
Department of Respiratory Medicine, Nanjing Children's Hospital, Nanjing Medical University, Nanjing 210029, China.
Qiu
Jie
J
Zhang
Chun-Mei
CM
Ji
Chen-Bo
CB
Guo
Xi-Rong
XR
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D007340:Insulinoma / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D052243:Resistin / Q000235:genetics / Q000502:physiology*; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics; D014162:Transfection
D052243:Resistin; C499622:Retn protein, rat; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D051057:Proto-Oncogene Proteins c-akt