Effect of the herbicide glyphosate on glyphosate-tolerant maize rhizobacterial communities: a comparison with pre-emergency applied herbicide consisting of a combination of acetochlor and terbuthylazine.
A comparison was made of the effect of glyphosate (RoundupPlus), a post-emergency applied herbicide, and of HarnessGTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplification, cloning and sequencing of soil DNA encoding 16S rRNA, rhizobacterial DNA hybridization to commercially available genome-wide microarrays from the soil bacterium Streptomyces coelicolor, and high-throughput DNA pyrosequencing of the bacterial DNA coding for 16S rRNA hypervariable V6 region. The results obtained strongly suggest that both herbicides do in fact affect the maize rhizobacterial communities, glyphosate being, to a great extent, the environmentally less aggressive herbicide.
Barriuso
Jorge
J
Centro Nacional de Biotecnología (CSIC), c/ Darwin 3, Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Marín
Silvia
S
Mellado
Rafael P
RP
D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology; D048372:Streptomyces coelicolor / Q000187:drug effects* / Q000235:genetics; D014052:Toluidines / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology; D003313:Zea mays / Q000382:microbiology*; D000097797:Glyphosate
D004269:DNA, Bacterial; D005998:Glycine; D006540:Herbicides; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D014052:Toluidines; D014227:Triazines; D000097797:Glyphosate; C043377:acetochlor; C037565:terbutylazine