[Detection of Helicobacter species related genes coding for 16S rRNA in the liver tissue of patients with chronic liver disease].
OBJECTIVE
To investigate the infection of Helicobacter pylori (Hp) in the liver tissue of patients with chronic liver disease and the association between Hp and chronic liver disease.


METHODS
Liver tissue samples were obtained by liver biopsy and surgical resection from 30 healthy subjects, 30 patients with chronic hepatitis, 30 with cirrhosis and 30 with liver cancer. All the samples were confirmed by pathological examination. The gene fragment coding for 16SrRNA were amplified by PCR with sequence analysis.


RESULTS
The PCR product of the 16SrRNA gene was 109 bp in length. Hp 16SrRNA was detected in 18 out of 30 liver biopsy samples from patients with primary cancer (60.0%), in 14 samples from patients with liver cirrhosis (47.0%), and in none of the samples from normal subjects or patients with chronic hepatitis. Sequencing analysis of Hp 16SrRNA gene in the liver tissue showed a 98.8% homology with the gene fragment encoding Hp 16SrRNA.


CONCLUSIONS
Hp is identified in the liver tissue of patients with chronic liver disease, suggesting the possible correlation between Hp infection and hepatocellular carcinoma.
Chen
Ren
R
Department of Infectious Diseases, Guangdong Provincial People's Hospital , Guangzhou 510080, China. crlmczx@yahoo.com.cn
Chen
Xiao-ping
XP
Lin
Qiu-qiong
QQ
Lin
Bing-liang
BL
Cao
Hui-juan
HJ
D000328:Adult; D000368:Aged; D001483:Base Sequence; D005260:Female; D016481:Helicobacter Infections / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006521:Hepatitis, Chronic / Q000150:complications / Q000382:microbiology*; D006801:Humans; D008103:Liver Cirrhosis / Q000150:complications / Q000382:microbiology*; D008113:Liver Neoplasms / Q000150:complications / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D055815:Young Adult
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S