Appearance of high-molecular weight sialoglycoproteins recognized by Maackia amurensis leukoagglutinin in gastric cancer tissues: a case report using 2-DE-lectin binding analysis.
Aberrant expression of sialoglycoconjugates has been thought to play an important role in cancer progression. Our previous lectin-histochemical study showed that overexpression of sialoglycoconjugates recognized by alpha2,3-sialic acid-specific Maackia amurensis leukoagglutinin (MAL) was significantly related to the malignancy of gastric cancer. The present study analyzed the sialoglycoproteins in gastric cancer tissues by 2-dimensional electrophoresis (2-DE) in combination with lectin-binding analysis using MAL. Various MAL-positive sialoglycoproteins were detected in cancer tissues but not in non-cancer tissues. The sialoglycoproteins have a high molecular weight of near 200 kDa and over 200 kDa with different pI values for the two. This suggests that the MAL-positive sialoglycoproteins detected in gastric cancer tissues have high molecular weights and may contain different numbers of alpha2,3-linked sialic acid residues in the carbohydrate moiety.
Inagaki
Yoshinori
Y
Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa, Japan.
Usuda
Mayumi
M
Xu
Huanli
H
Wang
Fengshan
F
Cui
Shuxiang
S
Mafune
Ken-ichi
K
Sugawara
Yasuhiko
Y
Kokudo
Norihiro
N
Tang
Wei
W
Nakata
Munehiro
M
D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D029901:Maackia / Q000737:chemistry*; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism*
D010835:Phytohemagglutinins; D012795:Sialoglycoproteins; C100156:leukoagglutinins, plants