title: Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as HPLC stationary phases.
abstract: Molecularly imprinted polymers (MIPs) of poly(ethylene glycol dimethacrylate) and poly(N-isopropylacrylamide-co-ethylene glycol dimethacrylate) were synthesized for the first time in supercritical carbon dioxide (scCO(2)), using Boc-L-tryptophan as template. Supercritical fluid technology provides a clean and one-step synthetic route for the preparation of affinity polymeric materials with sensing capability for specific molecules. The polymeric materials were tested as stationary HPLC phases for the enantiomeric separation of L- and D-tryptophan. HPLC results prove that the synthesized MIPs are able to recognize the template molecule towards its enantiomer which opens up potential applications in chromatographic chiral separation.
authors.affiliation: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Mara Soares
authors.initials: MS
authors.lastname: da Silva
authors.forename: Eva R
authors.initials: ER
authors.lastname: Vão
authors.forename: Márcio
authors.initials: M
authors.lastname: Temtem
authors.forename: Luís
authors.initials: L
authors.lastname: Mafra
authors.forename: Jorge
authors.initials: J
authors.lastname: Caldeira
authors.forename: Ana
authors.initials: A
authors.lastname: Aguiar-Ricardo
authors.forename: Teresa
authors.initials: T
authors.lastname: Casimiro
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007536:Isomerism; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D011108:Polymers / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D011108:Polymers; D014364:Tryptophan