[A transcriptional study of SDF-1alpha expression in PBL and the correlation between SDF-1alpha transcription and HIV-1 infection].
OBJECTIVE
To determine the transcription of SDF-1alpha in peripheral blood lymphocytes (PBL) and analysis the correlation between SDF-1alpha transcription and HIV infection.


METHODS
Three groups of study subjects were recruited: (1) 97 HIV negative healthy donors, (2) 92 HIV patients of A1 to A3 stages and (3) 146 HIV patients of B1 to C3 stages. Total RNA was extracted from PBL. Reverse transcription (RT)-PCR and quantification PCR were developed for the SDF-1alpha transcriptional study. R1 value was calculated based on the ratio of SDF-1alpha copies to beta-globin copies.


RESULTS
SDF-1alpha transcription is heterogeneous among the three study groups. The SDF-1alpha transcription was significantly up-regulated during late stage of HIV infection than the healthy donors. Correlation analysis indicated that R1 value was negatively correlated to CD4+ T cells counts (P = 0.002); and positively correlated to virus load (P = 0.001). The result demonstrated an association between SDF-1alpha transcription and disease progression.


CONCLUSIONS
SDF-1alpha transcription was significantly up-regulated during late stage of HIV infection. It would be worthwhile to determine the mechnism of HIV affecting on SDF-1alpha genes transcription and the up-regulated SDF-1alpha expression on the disease progression.
Zhao
Xiu-Ying
XY
Department of Clinical Laboratory, Beijing You-an Hospital, Capital Medical University, Beijing 100069, China. zhaoxy69@sohu.com
Li
Rui-Shan
RS
Huang
Jia-Qing
JQ
Chen
Zhi-Wei
ZW
Zheng
Bo-Jian
BJ
D016022:Case-Control Studies; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics* / Q000378:metabolism; D015658:HIV Infections / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000821:virology; D014158:Transcription, Genetic; D015854:Up-Regulation
D054377:Chemokine CXCL12