[Detecting significance of HBV-LP in the HBeAg negative patients with chronic hepatitis B].
OBJECTIVE
To explore the clinical significance of hepatitis B virus large envelope protein (HBV-LP) in diagnosing viral replication, we detected the HBV-LP and HBV DNA in the serum of the patients infected with HBV.


METHODS
Serum HBV DNA was detected by fluorescent quantitative PCR (FQ-PCR). HBV-LP was analyzed by enzyme linked immunosorbent assay (ELISA).


RESULTS
(1) No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the HBeAg negative patients without of anti-virus treatment. Of all HBeAg negative patients without of anti-virus treatment, the copies of HBV-DNA was related positively to the absorbency HBV-LP. (2) There was significant diference between the detectable rate of HBV DNA and HBV-LP in the HBeAg negative patients after anti-virus treatment.


CONCLUSIONS
There is a perfect correlation between the positive rate of HBV-LP and HBV DNA in the HBeAg negative patients.The HBV DNA is a reliable serological marker that can reflect the replication of HBV in the HBeAg negative patients.
Shi
Qian-feng
QF
The People's Hospital of Changxing, Zhejiang 313100, China.
Wang
Ye-ping
YP
D000328:Adult; D000368:Aged; D005260:Female; D006513:Hepatitis B e Antigens / Q000097:blood*; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000175:diagnosis / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014759:Viral Envelope Proteins / Q000097:blood*; D055815:Young Adult
D006513:Hepatitis B e Antigens; C096362:L protein, hepatitis B virus; D014759:Viral Envelope Proteins