Implication of glutathione S-transferase M1 and T1 polymorphisms in the development of senile cataract among Egyptians.
OBJECTIVE
To determine the effect of glutathione S-transferases M1 and T1 polymorphisms on the risk of senile cataract among Egyptians.


BACKGROUND
The glutathione S-transferases (GSTs) are polymorphic enzymes that are important in the protection against oxidative damage.


METHODS
Using a multiplex polymerase chain reaction (PCR), GSTM1 and GSTT1 gene polymorphisms were evaluated in 53 Egyptians with senile cataract and in 73 healthy individuals of the control group.


RESULTS
The frequency of GSTM1-positive individuals among the senile cataract group was significantly higher than in controls. The risk among the GSTM1-positive individuals of developing senile cataract was even higher in females. It is also increased with combination of "GSTM1-positive and GSTT1-positive" genotypes. However the combination of "GSTM1-null, GSTT1-positive" was found to be protective (OR = 0.47; 95 % CI: 0.22-0.99; p = 0.045).


CONCLUSIONS
The GSTMI-positive genotype and the combined "GSTM1-positive/GSTT1-positive" genotype may be associated with an increased risk of development of senile cataract among Egyptians. However, the "GSTM1-null/GSTT1-positive" genotype was found to be protective. Therefore, when evaluating the role of a particular GST gene in disease susceptibility, the whole pattern of different biotransformation enzymes should be taken into account (Tab. 4, Fig. 1, Ref. 36). Full Text (Free, PDF) www.bmj.sk.
Abdel Azeem
Amira A
AA
Research Institute of Ophthalmology, National Research Center, Cairo, Egypt. amiraazeem@hotmail.com
Mahmoud
Amany A
AA
Salaheldine
Mostafa M
MM
Amr
Khalda
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002386:Cataract / Q000235:genetics*; D034201:Computers, Handheld; D004534:Egypt; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1