Arcicella aurantiaca sp. nov., isolated from stream water.
A bacterial strain designated TNR-18(T) was isolated from stream water in southern Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TNR-18(T) were Gram-stain-negative, strictly aerobic, non-motile and vibrioid, and the strain formed orange colonies. Growth occurred at 10-30°C, with 0-0.2 % NaCl and at pH6.0-10.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-18(T) belonged to the genus Arcicella; its closest neighbours were Arcicella rosea TW5(T) and Arcicella aquatica NO-502(T), with respective sequence similarities of 97.9 and 97.8 %. Predominant cellular fatty acids (>10 %) were summed feature 3 (C(16 : 1)ω7c/C(16 : 1)ω6c; 37.7 %), C(16 : 1)ω5c (13.0 %) and iso-C(15 : 0) (11.1 %). The major respiratory quinone was MK-7. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized polar lipids. The DNA G+C content was 39.9&emsp14;mol%. On the basis of the phylogenetic and phenotypic data, strain TNR-18(T) should be classified as representing a novel species, for which the name Arcicella aurantiaca sp. nov. is proposed. The type strain is TNR-18(T) (=LMG 25207(T) =BCRC 17969(T)).
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Yang
Cho-Song
CS
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Chen
Ming-Hui
MH
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Arun
A B
AB
Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka, India.
Young
Chiu-Chung
CC
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7