Micropropagation and organogenesis of Anthurium andreanum Lind cv Rubrun.
Tissue culture techniques are routinely used for mass propagation and the establishment of disease free stock material. Virtually all pot type Anthuriums available in the market today are produced by tissue culture. In this chapter, we describe an efficient protocol to obtain Anthurium andreanum cv Rubrun vitro plants through micropropagation and organogenesis. Seeds from plant spadixes were germinated on MS medium supplemented with 0.5 mg/L BA. Micro-cuttings from in vitro germinated seedlings were subcultured on MS medium containing 2 mg/L BA and 0.5 mg/L NAA. Four-week-old in vitro plants obtained from microcuttings, showed callus proliferation at the stem base. The development of shoots and plantlets was observed from callus tissue. We also describe a detailed method for the histological analysis of callus tissue and a vitro plants acclimatization protocol.
Maira
Oropeza
O
Laboratorio de Mejoramiento Vegetal, Centro de Botánica Tropical, Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Los Chaguaramos, Caracas, Venezuela.
Alexander
Mejías
M
Vargas
Teresa Edith
TE
D000064:Acclimatization; D029064:Araceae / Q000196:embryology / Q000254:growth & development*; D049109:Cell Proliferation; D046508:Culture Techniques; D018525:Germination; D038081:Organogenesis; D018520:Plant Shoots / Q000254:growth & development; D012098:Reproduction; D036226:Seedlings / Q000254:growth & development; D012639:Seeds; D013997:Time Factors