Rapid chromatographic immunoassay study of brain PrPsc distribution in classical scrapie.
In the current study, a rapid chromatographic immunoassay submitted for registration in Europe was used to analyze PrP(sc) in 13 different areas of brain from 10 confirmed classical scrapie cases. The levels of PrP(sc) in the different areas of brain were plotted to draw a brain PrP(sc) distribution curve. This curve was compared with the brain PrP(sc) distribution curve obtained from immunoblotting and immunohistochemistry tests on the same samples. The distribution of PrP(sc) in different areas of the brain was similar, irrespective of the test applied, indicating that any of the 3 tests could be used for the characterization of classical cases of scrapie.
Bolea
Rosa
R
PRIOCAT Laboratory, Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
Vidal
Enric
E
Marín
Belén
B
Márquez
Mercedes
M
Vargas
Antonia
A
Pumarola
Martí
M
Badiola
Juan José
JJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002845:Chromatography / Q000379:methods / Q000662:veterinary*; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D018620:PrPSc Proteins / Q000032:analysis*; D012608:Scrapie / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012756:Sheep
D018620:PrPSc Proteins