title: Quantitative determination of flavonoids and cycloartanol glycosides from aerial parts of Sutherlandia frutescens (L.) R. BR. by using LC-UV/ELSD methods and confirmation by using LC-MS method.
abstract: This paper describes the first analytical method for the determination of four flavonoids (sutherlandins A-D) and four cycloartanol glycosides (sutherlandiosides A-D) from the aerial parts of Sutherlandia frutescens (L.) R. Br. A separation by HPLC was achieved by using a reversed phase (RP-18) column, PDA with ELS detection, and a water/acetonitrile gradient as the mobile phase. The wavelength used for quantification of four flavonoids with the diode array detector was 260 nm. Owing to their low UV absorption, the cycloartanol glycosides were detected by evaporative light scattering. The method was validated for linearity, repeatability, limits of detection (LOD) and limits of quantification (LOQ). The limits of detection and limits of quantification of eight compounds were found to be in the range from 0.1 to 7.5 microg/mL and 0.5 to 25 microg/mL, respectively. The analysis of products showed considerable variation of 1.099-5.224 mg/average weight for the major compound, sutherlandioside B. The eight compounds in plant sample and products of S. frutescens were further confirmed by LC-ESI-TOF. This method involved the use of the [M+H](+) and [M+Na](+) ions in the positive ion mode with extractive ion monitoring (EIM).
authors.affiliation: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677, USA. bavula@olemiss.edu
authors.forename: Bharathi
authors.initials: B
authors.lastname: Avula
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Troy J
authors.initials: TJ
authors.lastname: Smillie
authors.forename: Xiang
authors.initials: X
authors.lastname: Fu
authors.forename: Xing Cong
authors.initials: XC
authors.lastname: Li
authors.forename: Wilfred
authors.initials: W
authors.lastname: Mabusela
authors.forename: James
authors.initials: J
authors.lastname: Syce
authors.forename: Quinton
authors.initials: Q
authors.lastname: Johnson
authors.forename: William
authors.initials: W
authors.lastname: Folk
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D007887:Fabaceae; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D008027:Light; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D005419:Flavonoids; D006027:Glycosides