title: Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test.
abstract: The new EU legislations for cosmetics (Seventh Amendment) have laid down deadlines for the replacement of animal tests in cosmetics. This policy stimulates the acceptance of in vitro approaches to test embryotoxic potentials of compounds in cosmetics products. The embryonic stem cell test (EST) designed by The European Centre for the Validation of Alternative Methods (ECVAM) is currently the most promising in vitro assay to predict the embryotoxic potential of compounds. In this study, six selected compounds (hydroquinone, eugenol, dibutyl phthalate, antimony (III) oxide, neodymium (III) nitrate hydrate, melamine) formerly involved in cosmetic products were selected to test their embryotoxic potentials by the EST. 5-Fluorouracil and penicillin G were separately set as positive and negative control. The embryotoxic potential was determined by the prediction model (PM), which was calculated from three endpoints, the IC(50) 3T3, IC(50) ES, and ID(50). Hydroquinone, eugenol, and antimony (III) oxide indicated with strong embryotoxicity, while dibutyl phthalate, neodymium (III) nitrate hydrate, and melamine exhibited a weak embryotoxicity. These results may provide a valuable attempt to expand the application of EST to the cosmetics field.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, 74# Zhongshan Road 2, Guangzhou, Guangdong. PR China 510080.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Shujun
authors.initials: S
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Li
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Jiao
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.forename: Bruce T
authors.initials: BT
authors.lastname: Lahn
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
mesh_terms: D000818:Animals; D000965:Antimony / Q000633:toxicity; D002460:Cell Line; D003358:Cosmetics / Q000633:toxicity*; D003993:Dibutyl Phthalate / Q000633:toxicity; D053595:Embryonic Stem Cells / Q000187:drug effects*; D005054:Eugenol / Q000633:toxicity; D006873:Hydroquinones / Q000633:toxicity; D051379:Mice; D009354:Neodymium / Q000633:toxicity; D010087:Oxides / Q000633:toxicity; D013723:Teratogens / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*; D014227:Triazines / Q000633:toxicity
chemical_list: D003358:Cosmetics; D006873:Hydroquinones; D010087:Oxides; D013723:Teratogens; D014227:Triazines; C091696:antimony oxide; D003993:Dibutyl Phthalate; D009354:Neodymium; D005054:Eugenol; D000965:Antimony; C011907:melamine; C031927:hydroquinone