Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.
OBJECTIVE
This study was undertaken to test injectable surgical sealants that are biocompatible with fetal membranes and that are to be used eventually for the closure of iatrogenic membrane defects.


METHODS
Dermabond (Ethicon Inc, Norderstedt, Germany), Histoacryl (B. Braun GmbH, Tuttlingen, Germany), and Tissucol (Baxter AG, Volketwil, Switzerland) fibrin glue, and 3 types of in situ forming poly(ethylene glycol)-based polymer hydrogels were tested for acute toxicity on direct contact with fetal membranes for 24 hours. For the determination of elution toxicity, extracts of sealants were incubated on amnion cell cultures for 72 hours. Bonding and toxicity was assessed through morphologic and/or biochemical analysis.


RESULTS
Extracts of all adhesives were nontoxic for cultured cells. However, only Tissucol and 1 type of poly(ethylene glycol)-based hydrogel, which is a mussel-mimetic tissue adhesive, showed efficient, nondisruptive, nontoxic bonding to fetal membranes. Mussel-mimetic tissue adhesive that was applied over membrane defects that were created with a 3.5-mm trocar accomplished leak-proof closure that withstood membrane stretch in an in vitro model.


CONCLUSIONS
A synthetic hydrogel-type tissue adhesive that merits further evaluation in vivo emerged as a potential sealing modality for iatrogenic membrane defects.
Bilic
Grozdana
G
Department of Obstetrics, University Hospital Zurich, Switzerland.
Brubaker
Carrie
C
Messersmith
Phillip B
PB
Mallik
Ajit S
AS
Quinn
Thomas M
TM
Haller
Claudia
C
Done
Elisa
E
Gucciardo
Leonardo
L
Zeisberger
Steffen M
SM
Zimmermann
Roland
R
Deprest
Jan
J
Zisch
Andreas H
AH
D000650:Amnion / Q000166:cytology / Q000187:drug effects* / Q000601:surgery*; D003487:Cyanoacrylates / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D004659:Enbucrilate / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005322:Premature Rupture of Fetal Membranes; D005332:Fetoscopy; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels / Q000008:administration & dosage / Q000627:therapeutic use*; D066298:In Vitro Techniques; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D014014:Tissue Adhesives / Q000008:administration & dosage / Q000494:pharmacology*
D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D020100:Hydrogels; D014014:Tissue Adhesives; D011092:Polyethylene Glycols; C100832:octyl 2-cyanoacrylate; D004659:Enbucrilate