title: Induction of indistinguishable gene expression patterns in rats by Vero cell-derived and mouse brain-derived Japanese encephalitis vaccines.
abstract: Transcriptomics is an objective index that reflects the overall condition of cells or tissues, and transcriptome technology, such as DNA microarray analysis, is now being introduced for the quality control of medical products. In this study, we applied DNA microarray analysis to evaluate the character of Japanese encephalitis (JE) vaccines. When administered into rat peritoneum, Vero cell-derived and mouse brain-derived JE vaccines induced similar gene expression patterns in liver and brain. Body weights and blood biochemical findings were also similar after administration of the two vaccines. Our results suggest that the two JE vaccines are likely to have equivalent characteristics with regard to reactivity in rats.
authors.affiliation: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Momose
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Imai
authors.forename: Isao
authors.initials: I
authors.lastname: Hamaguchi
authors.forename: Mika
authors.initials: M
authors.lastname: Kawamura
authors.forename: Takuo
authors.initials: T
authors.lastname: Mizukami
authors.forename: Seishiro
authors.initials: S
authors.lastname: Naito
authors.forename: Atsuko
authors.initials: A
authors.lastname: Masumi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Maeyama
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takizawa
authors.forename: Madoka
authors.initials: M
authors.lastname: Kuramitsu
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nomura
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D001835:Body Weight; D001921:Brain / Q000276:immunology / Q000821:virology*; D002522:Chlorocebus aethiops; D018349:Encephalitis Viruses, Japanese / Q000254:growth & development* / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000517:prevention & control*; D020869:Gene Expression Profiling; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D014709:Vero Cells
chemical_list: D022321:Japanese Encephalitis Vaccines