[Activity of selected antioxidant enzymes examination in people with colorectal cancer].
Colorectal cancer is a serious medical problem. According to one hypothesis of carcinogenesis, tumour creation process begins and develops when the balance between the production of reactive oxygen species (ROS) and their disactivation by the antioxidant protective barrier of the organism is disturbed. THE AIM OF THE STUDY was, following this theory, to examine the plasma concentration of the dietary minerals with antioxidant properties.


METHODS
The research material was the blood of 8 people, taken to vacuum test tubes with an anticoagulant - 4 ml of sodium heparin. The activity of catalase was marked following Beers method, while the activity of superoxide dismutase according to H.P. Misra and J. Fridovich method.


RESULTS
The results show that the copper complex added, i.e. dichlorine(3,4,5-trimethyl-N1 - pyrazol-1yl) Cu(II) from derivatives of azoles, has a large impact on the activity of selected enzymes. After adding it, their activity increased by ca 67% in comparison with the control group. It proves the efficacy of this compound.


CONCLUSIONS
The results prove the free radicals theory of carcinogenesis. They also confirm the part which the antioxidant protective barrier plays in the defense against ROS and their carcinogenic consequences.
Klimczak
Alicja
A
Uniwersytet Medyczny w Łodzi, Wydział Fizjoterapii, III Katedra Rehabilitacji, Zakład Historii Nauk i Medycyny Wojskowej. ala_klimczak@o2.pl
Kubiak
Katarzyna
K
Malinowska
Katarzyna
K
Dziki
Łukasz
Ł
D015179:Colorectal Neoplasms / Q000097:blood / Q000201:enzymology*; D003300:Copper / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009942:Organometallic Compounds / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood*
D005609:Free Radicals; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; C546138:dinitrate (V) di(3,4,5-trimethyl-N'-pyrazol-kappaN(2)) copper(II); D003300:Copper; D013482:Superoxide Dismutase