title: Chemical structure of Bacteriovorax stolpii lipid A.
abstract: Bdellovibrionales is a phylogenetically diverse group of predatory prokaryotes, which consists of the two families Bdellovibrionaceae and Bacteriovoracaceae. We describe LPS and lipid A of the type strain Bacteriovorax stolpii DSM 12778, representing the first characterized endotoxin of a Bacteriovoracaceae member. It has a smooth form LPS, which was identified by SDS-polyacrylamide gel electrophoresis. The lipid A structure was determined by combined gas chromatography-mass spectrometry, electrospray ionization mass spectrometry and NMR spectroscopy. Its backbone consists of two beta-(1-->6)-linked 2,3-diamino-2,3-dideoxy-D-glucopyranoses (GlcpN3N) carrying a pyrophosphoethanolamine at O-4' of the non-reducing sugar and a phosphate group linked to O-1 of the reducing GlcpN3N. Positions 2, 3, 2' and 3' of the two GlcpN3N are acylated with primary 3-hydroxy fatty acids and one of those carries a secondary fatty acid.
authors.affiliation: Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Beck
authors.forename: Frederic D
authors.initials: FD
authors.lastname: Müller
authors.forename: Eckhard
authors.initials: E
authors.lastname: Strauch
authors.forename: Lothar
authors.initials: L
authors.lastname: Brecker
authors.forename: Michael W
authors.initials: MW
authors.lastname: Linscheid
mesh_terms: D020564:Deltaproteobacteria / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005227:Fatty Acids; D008050:Lipid A