Viability and functional capacity after thawing of hematopoietic progenitor cells cryopreserved at a cord blood stem cell bank in Colombia.
OBJECTIVE
To evaluate the viability and functional capacity of hematopoietic progenitor cells from cord blood samples cryopreserved at the Banco de Células Stem de Colombia.


METHODS
After thawing and centrifugation of 20 samples, viable white blood cells were numbered by the trypan blue method and CD34(+)CD45(+dim) hematopoietic progenitor cells were numbered by flow cytometry. Clonogenic assays also tested the functional capacity of viable CD34(+)CD45(+dim) cells.


RESULTS
The median rates of viable CD34(+)CD45(+dim) cells were 99.6% before freezing and 73.0% after thawing (P<0.001). The 20 cultures yielded a median of 12 cells with a lineage of red cells, 17.5 cells with a lineage of white cells, and 10 cells with a mixed lineage.


CONCLUSIONS
Although the rate of viable CD34(+)CD45(+dim) cells was decreased by 26.6% after thawing by the method we used, the numbers of CD34(+)CD45(+dim) cells that formed colonies were similar to those obtained by other published methods.
Avila Portillo
Luz Mabel
LM
Banco de Células Stem de Colombia, Bogotá, Colombia. laboratorio@bancodecelulas.com
Guzmán
Juliana
J
Rojas
Carolina
C
Becerra
Andrea
A
Avila
Jennifer
J
Jaimes
Diego
D
Ospino
Benjamín
B
Gomez
Claudia
C
Madero
José Ignacio
JI
D018952:Antigens, CD34 / Q000032:analysis; D002470:Cell Survival; D003105:Colombia; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D011247:Pregnancy
D018952:Antigens, CD34; D017493:Leukocyte Common Antigens