Aluminum uptake and disease resistance in Nicotiana rustica leaves.
The comparative effectiveness of aluminum hydroxide and aluminum chloride has been studied in the development of bacterial wilt infection on leaves of Nicotiana rustica cv. Gansu yellow flower. We have analyzed the changes of foliar H(2)O(2) content, as well as of non-enzymatic and enzymatic antioxidants under aluminum stress. Pretreatment with aluminum hydroxide before pathogen challenge reduced the development of Ralstonia solanacearum infection and decreased the extent of leaf injury. The pretreatment also reduced the Al uptake in comparison to pretreatment with aluminum chloride. H(2)O(2) generation was significantly enhanced by pretreatment with aluminum hydroxide. Increased NADPH oxidase and superoxide dismutase activities were correlated with limited infection. Aluminum hydroxide pretreatment shifted the leaf redox homeostasis of AsA/DHA and GSH/GSSG toward oxidation, yielding higher oxidant levels than aluminum chloride before bacterial inoculation. The results support the idea that aluminum hydroxide induced H(2)O(2) accumulation through non-enzymatic and enzymatic regulation, ultimately resulting in resistance to tobacco wilt disease.
Zhang
Bo
B
MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China.
Wang
Xiao-qin
XQ
Li
Xin
X
Ni
Yong-qing
YQ
Li
Hong-yu
HY
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000378:metabolism* / Q000494:pharmacology; D000536:Aluminum Hydroxide / Q000378:metabolism* / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D019255:NADPH Oxidases / Q000032:analysis / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D043368:Ralstonia solanacearum / Q000254:growth & development; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology
D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D000536:Aluminum Hydroxide; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D019255:NADPH Oxidases