Protective effects of luteolin-7-O-beta-D-glucuronide methyl ester from the ethyl acetate fraction of Lycopi Herba against pro-oxidant reactive species and low-density lipoprotein peroxidation.
In this study the potent scavenging activity of "Lycopi Herba" (LH) extract was studied using the following: evaluation of the total phenolics, measuring the antioxidant activity by Trolox equivalent antioxidant concentration, measuring the scavenging effects on reactive oxygen species, on reactive nitrogen species, and measuring the inhibitory effect on Cu(2+) induced human low-density lipoprotein oxidation in vitro. The ethyl acetate fraction from the LH extracts were found to have a potent scavenging activity against all of the reactive species tested, as well as an inhibitory effect on LDL oxidation. Therefore, we isolated and identified luteolin-7-O-beta-D-glucuronide methyl ester as the major compound from the ethyl acetate fraction of LH and their antioxidant activities were evaluated.
Lee
Min-Ja
MJ
Cardiovascular Medical Research Center and Department of Diagnostics, College of Oriental Medicine, Dongguk University, Sukjang-Dong 707, Gyeong-Ju 780-714, Gyeongbuk, Republic of Korea.
Lee
Hye-Sook
HS
Park
Sun-Dong
SD
Moon
Hyung-In
HI
Park
Won-Hwan
WH
D000085:Acetates; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D005591:Chemical Fractionation; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D020719:Glucuronides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000032:analysis / Q000737:chemistry; D047311:Luteolin / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D031333:Lycopus / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D026361:Reactive Nitrogen Species / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012997:Solvents; D014934:Wood / Q000737:chemistry
D000085:Acetates; D000975:Antioxidants; D016166:Free Radical Scavengers; D020719:Glucuronides; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D012997:Solvents; C467205:luteolin-7-glucuronide methyl ester; C099252:oxidized low density lipoprotein; C007650:ethyl acetate; D047311:Luteolin