Human serum albumin binding ibuprofen: a 3D description of the unfolding pathway in urea.
Small angle X-ray scattering (SAXS) technique, supported by light scattering measurements and spectroscopic data (circular dichroism and fluorescence) allowed us to restore the 3D structure at low resolution of defatted human serum albumin (HSA) in interaction with ibuprofen. The data were carried out on a set of HSA solutions with urea concentrations between 0.00 and 9.00M. The Singular Value Decomposition method, applied to the complete SAXS data set allowed us to distinguish three different states in solution. In particular a native conformation N (at 0.00M urea), an intermediate I1 (at 6.05M urea) and an unfolded structure U (at 9.00M urea) were recognized. The low-resolution structures of these states were obtained by exploiting both ab initio and rigid body fitting methods. In particular, for the protein without denaturant, a conformation recently described (Leggio et al., PCCP, 2008, 10, 6741-6750), very similar to the crystallographic heart shape, with only a slight reciprocal movement of the three domains, was confirmed. The I1 structure was instead characterized by only a closed domain (domain III) and finally, the recovered structure of the U state revealed the characteristic feature of a completely open state. A direct comparison with the free HSA pointed out that the presence of the ibuprofen provokes a shift of the equilibrium towards higher urea concentrations without changing the unfolding sequence. The work represents a type of analysis which could be exploited in future investigations on proteins in solution, in the binding of drugs or endogenous compounds and in the pharmacokinetic properties as well as in the study of allosteric effects, cooperation or anticooperation mechanisms.
Galantini
Luciano
L
Dipartimento di Chimica, Sapienza Università di Roma, Piazzale A. Moro 5, Rome, Italy. l.galantini@caspur.it
Leggio
Claudia
C
Konarev
Peter V
PV
Pavel
Nicolae V
NV
D001665:Binding Sites; D006801:Humans; D007052:Ibuprofen / Q000737:chemistry*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects; D053838:Scattering, Small Angle; D012709:Serum Albumin / Q000737:chemistry*; D014508:Urea / Q000737:chemistry* / Q000494:pharmacology; D014961:X-Ray Diffraction
D012709:Serum Albumin; D014508:Urea; D007052:Ibuprofen