title: PCA3 gene expression in prostate cancer tissue in a Chinese population: quantification by real-time FQ-RT-PCR based on exon 3 of PCA3.
abstract: Prostate cancer (PCa) is the second most common cancer in men, and its incidence is still increasing. PCA3 is the most prostate cancer specific biomarker. Here we confirmed that both exon 3 and exon 4 are in the prostate-specific region of the PCA3 gene, and established the methodology of real-time fluorescent quantitative RT-PCR (FQ-RT-PCR) detecting PCA3 mRNA with primer spanning exons 1 and 3, and evaluated its clinical utility in a Chinese population. What disclosed that PCA3 mRNA is prostate cancer specific and shows increased expression in prostate cancer. It could be a reliable molecular marker in prostate cancer diagnosis. Exon 3-based real-time FQ-RT-PCR may prove useful in prostate cancer diagnosis, given that the associated primer would span only exons 1 and 3, relative to other models spanning exons 1 to 4. A shorter amplicon would not only enhance the efficiency of real-time FQ-RT-PCR, but may also simplify the quantification of PCA3 mRNA.
authors.affiliation: Department of Medical Laboratory, Chongqing Medical University, Chongqing, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Tao
authors.forename: Mo
authors.initials: M
authors.lastname: Shen
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Mao
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Chen
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Yu
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Weng
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Chengdi
authors.initials: C
authors.lastname: Li
authors.forename: Xiuling
authors.initials: X
authors.lastname: Wu
authors.forename: Yuanping
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Ouchen
authors.initials: O
authors.lastname: Wang
authors.forename: Qitong
authors.initials: Q
authors.lastname: Song
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D011470:Prostatic Hyperplasia / Q000235:genetics* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C479341:prostate cancer antigen 3, human