On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine.
A new assay for urinary homocysteine is described. The assay relies on an on-column derivatization with o-phthaldialdehyde, using a reversed-phase HPLC column and detection/quantification by fluorescence. The analysis time for reduced and total homocysteine, including sample work-up, was 5 and 13 min, respectively. Quantification limit was 25 nM.
Głowacki
Rafał
R
Department of Environmental Chemistry, University of Łódź, 90-131 Łódź, Poland.
Borowczyk
Kamila
K
Bald
Edward
E
Jakubowski
Hieronim
H
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006710:Homocysteine / Q000652:urine*; D006801:Humans; D009764:o-Phthalaldehyde / Q000737:chemistry
D006710:Homocysteine; D009764:o-Phthalaldehyde