title: Low-cost fluorimetric determination of radicals based on fluorogenic dimerization of the natural phenol sesamol.
abstract: A novel fluorimetric method for determining radicals using the natural phenol sesamol as a fluorogenic reagent is reported. In this assay, sesamol was reacted with aqueous radicals to yield one isomer of a sesamol dimer exclusively. The dimer emitted purple fluorescence near 400 nm around neutral pH, where it assumed the monoanionic form. This method was applied to the straightforward detection of radical nitric oxide (NO). The ready availability of sesamol should enable rapid implementation of applications utilizing this new assay, particularly in high-throughput analysis or screening.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Makino
authors.forename: Seiichi
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
mesh_terms: D000042:Absorption; D000840:Anisoles / Q000737:chemistry; D052117:Benzodioxoles / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry*; D002021:Buffers; D019281:Dimerization; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000191:economics* / Q000379:methods*; D005609:Free Radicals / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D009569:Nitric Oxide / Q000032:analysis / Q000737:chemistry; D010087:Oxides / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D000840:Anisoles; D052117:Benzodioxoles; D001688:Biological Products; D002021:Buffers; D005456:Fluorescent Dyes; D005609:Free Radicals; D010087:Oxides; D010636:Phenols; D012997:Solvents; D014867:Water; C052893:tert-butoxy; D009569:Nitric Oxide; C025583:sesamol; C060998:anisole