[Biodiversity of Lactobacilli from fermented milk products in Northern Tibet].
OBJECTIVE
In order to study the biodiversity of Lactobacilli from Northern Tibet in China, we developed enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR) technique.


METHODS
Seventy seven Lactobacillus strains were isolated from traditional fermented milk products which are made by herd man families in Northern Tibet of China. By ERIC-PCR technique method, using NTSYS-pc2.1 software, we investigated the biodiversity of the strains.


RESULTS
The amplified bands of ERIC-PCR were clear and stable with high repetition and high polymorphisms. The cluster analysis showed that at level 0.73, 77 tested strains were clustered into 4 groups: A1: strains of Lactobacillus casei (35.06% of the strains), A2: strains of Lactobacillus fermentum (61.04% of the strains), A3: strains of Lactobacillus helveticus and A4: strains of Lactobacillus plantarum. The biodiversity of the isolates from Aland A2 groups was further investigated. A1 could be divided into 5 genotypes at the similarity of 80.0%. A2 could be divided into 6 genotypes at the similarity of 84.0%.


CONCLUSIONS
Using ERIC-PCR technique, Lactobacilli from Northern Tibet could be distinguished at species level.
Ju
Ning
N
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China. jn2005282@sina.com
Zhang
Jiachao
J
Sun
Zhihong
Z
Liu
Wenjun
W
YeJin
Zhang
Heping
H
Sun
Tiansong
T
D044822:Biodiversity; D002681:China; D043302:Cultured Milk Products / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018609:Tibet