Inhibitory effects of fermented grape marc from Vitis vinifera Negroamaro on antigen-induced degranulation.
To investigate the antiallergic effects of fermented grape marc from Negroamaro (N-FGM), we examined antigen (Ag)-induced degranulation of rat basophilic leukemia (RBL-2H3) cells. Among supernatants of N-FGM suspensions in water, ethanol, and dimethyl sulfoxide (DMSO), supernatants of DMSO-suspended N-FGM but not of nonfermented Negroamaro grape marc (N-GM) markedly suppressed the Ag-induced degranulation and phosphorylation of Syk in RBL-2H3 cells. Supernatants of DMSO-suspended N-FGM did not reduce the expression of FcepsilonRI on RBL-2H3 cells. Analyses of supernatants of N-FGM suspensions in water, ethanol, and DMSO by high-performance liquid chromatography revealed higher amounts of quercetin in supernatants of DMSO-suspended N-FGM than those in the other supernatants. Quercetin also suppressed the Ag-induced degranulation and phosphorylation of Syk but did not reduce the expression of FcepsilonRI on RBL-2H3 cells. These results suggest that inhibition of the Ag-induced degranulation and Syk phosphorylation by N-FGM might be due to the action of quercetin, as an active component in N-FGM.
Kaneko
Masahiro
M
Department of Biosciences, School of Science, Kitasato University, 1-15-1 Sagamihara, Kanagawa 228-8555, Japan. kanekom@sci.kitasato-u.ac.jp
Kanesaka
Manabu
M
Yoneyama
Miho
M
Tominaga
Takanari
T
Jirillo
Emilio
E
Kumazawa
Yoshio
Y
D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology; D015549:Basophil Degranulation Test; D001491:Basophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D005285:Fermentation; D007073:Immunoglobulin E / Q000276:immunology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D000072377:Syk Kinase; D014407:Tumor Cells, Cultured; D027843:Vitis / Q000737:chemistry*; D014920:Wine
D018926:Anti-Allergic Agents; D000941:Antigens; D047908:Intracellular Signaling Peptides and Proteins; D007073:Immunoglobulin E; D011794:Quercetin; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606898:Syk protein, rat