Diversity of fungi in creosote-treated crosstie wastes and their resistance to polycyclic aromatic hydrocarbons.
This study was conducted to generate information regarding the diversity of fungi inhabiting creosote-treated wood in a storage yard for crosstie wastes in Gwangmyeong, Korea. Additionally, the resistance to polycyclic aromatic hydrocarbons (PAHs) of indigenous fungi that mainly occupy creosote-treated wood was evaluated. We isolated fungi from the surface and inner area of crosstie wastes and identified them using a combination of traditional methods and molecular techniques. Overall, 179 isolates including 47 different species were isolated from 240 sampling sites. The identified fungal species included 23 ascomycetes, 19 basidiomycetes, and 5 zygomycetes. Three species, Alternaria alternata, Irpex lacteus, and Rhizomucor variabilis, were the most frequently isolated ascomycetes, basidiomycetes, and zygomycetes, respectively. The results of this study showed that there was a large difference in the fungal diversity between the surface and the inner area. Additionally, zygomycetes and ascomycetes were found to have a greater tolerance to PAHs than basidiomycetes. However, two basidiomycetes, Heterobasidion annosum and Schizophyllum commune, showed very high resistance to PAHs, even in response to the highest concentration (1,000 ppm), which indicates that these species may play a role in the degradation of PAHs.
Kim
Min-Ji
MJ
Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, Korea.
Lee
Hwanhwi
H
Choi
Yong-Seok
YS
Kim
Gyu-Hyeok
GH
Huh
Na-Yoon
NY
Lee
Sangjoon
S
Lim
Young Woon
YW
Lee
Sung-Suk
SS
Kim
Jae-Jin
JJ
D000935:Antifungal Agents / Q000633:toxicity*; D044822:Biodiversity; D016000:Cluster Analysis; D003407:Creosote; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D020459:Genes, rRNA; D007220:Industrial Waste; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012331:RNA, Fungal / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014934:Wood / Q000382:microbiology*
D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D012331:RNA, Fungal; D012339:RNA, Ribosomal, 28S; D003407:Creosote