Effect of hydration on silk film material properties.
Effects of hydration on silk fibroin film properties were investigated for water-annealed and MeOH-treated samples. Hydration increased thickness by 60% for MeOH-immersed films, while water-annealed samples remained constant. MeOH-immersed films showed an 80% mass loss due to water, while water-annealed lost only 40%. O(2) permeability was higher in MeOH-immersed films with Dk values of 10(-10) (mL O(2) x cm) x (cm(-1) x s(-1) x mmHg(-1)), while those of water-annealed films reached only one fifth of this value. All films showed a decrease in Young's modulus and increased plastic deformation by two orders of magnitude when submerged in saline solution. FT-IR showed that beta-sheet content in water-annealed films increased with increasing water vapor pressure, while MeOH-immersed films showed no change.
Lawrence
Brian D
BD
Department of Biomedical Engineering, Cornell University, 101 Weill Hall, Ithaca, NY 14853, USA.
Wharram
Scott
S
Kluge
Jonathan A
JA
Leisk
Gary G
GG
Omenetto
Fiorenzo G
FG
Rosenblatt
Mark I
MI
Kaplan
David L
DL
D000042:Absorption; D001672:Biocompatible Materials / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry; D006358:Hot Temperature; D008422:Materials Testing; D000432:Methanol / Q000737:chemistry; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008958:Models, Molecular; D010100:Oxygen / Q000737:chemistry; D010539:Permeability; D017433:Protein Structure, Secondary; D012212:Rheology; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D047011:Silk; D014867:Water; D005349:Fibroins; D010100:Oxygen; D000432:Methanol