title: DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae encodes an RNA-binding protein from the pentatricopeptide repeat family required for the maintenance of the mitochondrial 15S ribosomal RNA.
abstract: Pentatricopeptide repeat (PPR) proteins form the largest known RNA-binding protein family and are found in all eukaryotes, being particularly abundant in higher plants. PPR proteins localize mostly in mitochondria and chloroplasts, where they modulate organellar genome expression on the post-transcriptional level. The Saccharomyces cerevisiae DMR1 (CCM1, YGR150C) encodes a PPR protein that localizes to mitochondria. Deletion of DMR1 results in a complete and irreversible loss of respiratory capacity and loss of wild-type mtDNA by conversion to rho(-)/rho(0) petites, regardless of the presence of introns in mtDNA. The phenotype of the dmr1Delta mitochondria is characterized by fragmentation of the small subunit mitochondrial rRNA (15S rRNA), that can be reversed by wild-type Dmr1p. Other mitochondrial transcripts, including the large subunit mitochondrial rRNA (21S rRNA), are not affected by the lack of Dmr1p. The purified Dmr1 protein specifically binds to different regions of 15S rRNA in vitro, consistent with the deletion phenotype. Dmr1p is therefore the first yeast PPR protein, which has an rRNA target and is probably involved in the biogenesis of mitochondrial ribosomes and translation.
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Olga
authors.initials: O
authors.lastname: Puchta
authors.forename: Michal
authors.initials: M
authors.lastname: Lubas
authors.forename: Kamil A
authors.initials: KA
authors.lastname: Lipinski
authors.forename: Jakub
authors.initials: J
authors.lastname: Piatkowski
authors.forename: Michal
authors.initials: M
authors.lastname: Malecki
authors.forename: Pawel
authors.initials: P
authors.lastname: Golik
mesh_terms: D020816:Amino Acid Motifs; D001483:Base Sequence; D019069:Cell Respiration; D003593:Cytoplasm / Q000378:metabolism; D005095:Exoribonucleases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D013379:Substrate Specificity
chemical_list: C545698:Ccm1 protein, S cerevisiae; D024101:Mitochondrial Proteins; C077890:PET127 protein, S cerevisiae; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D005095:Exoribonucleases; C028793:5'-exoribonuclease