Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI.
Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI.
Rawe
Vanesa Y
VY
Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina. vrawe@cegyr.com
Boudri
Heydy Uriondo
HU
Alvarez Sedó
Cristian
C
Carro
Martina
M
Papier
Sergio
S
Nodar
Florencia
F
D000328:Adult; D017304:Annexin A5; D002469:Cell Separation / Q000379:methods*; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D007231:Infant, Newborn; D007248:Infertility, Male / Q000628:therapy*; D008280:Magnetics; D008297:Male; D008863:Microspheres; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D017304:Annexin A5