Evaluation of a new experimental kit for the extraction of DNA from bones and teeth using a non-powder method.
An experimental DNA extraction kit (new kit) was recently developed to extract DNA from degraded skeletal remains without the need for powdering the samples. We compared the utility of the new kit with the conventional phenol/chloroform method using real-time quantitative PCR and multiplex STR analysis. The new kit yielded large amounts of DNA from a compact bone fragment compared with the conventional phenol/chloroform method. We were able to extract sufficient DNA for STR analysis from 75% (3 of 4) and 60% (3 of 5) of the un-powdered tooth and bone samples, respectively, using the new kit. We were able to perform mini-STR analysis of the remaining samples using DNA extracted with the new kit. Furthermore, we successfully performed mitochondrial DNA sequencing of every sample. The new kit simplifies the DNA extraction procedure as it does not require powdering samples. Decreasing the number of procedural steps in DNA extraction will be beneficial in controlling DNA contamination in laboratories. Our results suggest that the new kit may be used for the simple, simultaneous extraction of DNA from multiple samples.
Kitayama
Tetsushi
T
First Department of Forensic Science, National Research Institute of Police Science, Chiba 277-0882, Japan. tetsushik@nrips.go.jp
Ogawa
Yoshinori
Y
Fujii
Koji
K
Nakahara
Hiroaki
H
Mizuno
Natsuko
N
Sekiguchi
Kazumasa
K
Kasai
Kentaro
K
Yurino
Noriko
N
Yokoi
Takahide
T
Fukuma
Yoshiya
Y
Yamamoto
Kenji
K
Oki
Takahito
T
Asamura
Hideki
H
Fukushima
Hirofumi
H
D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology*; D004247:DNA / Q000302:isolation & purification*; D003648:Decalcification Technique; D018732:Forensic Anthropology / Q000379:methods*; D053803:Forensic Genetics; D006801:Humans; D017422:Sequence Analysis, DNA; D014070:Tooth / Q000378:metabolism* / Q000473:pathology*
D004247:DNA