A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. extralong autumn purple bean.
Lectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV-1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy.
Fang
Evandro Fei
EF
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Lin
Peng
P
Wong
Jack Ho
JH
Tsao
Sai Wah
SW
Ng
Tzi Bun
TB
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D007887:Fabaceae / Q000737:chemistry*; D054303:HIV Reverse Transcriptase / Q000302:isolation & purification*; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000235:genetics; D027805:Phaseolus / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons
D004791:Enzyme Inhibitors; D037102:Lectins; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II; D054303:HIV Reverse Transcriptase