title: A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.
abstract: BACKGROUND
  Pancreas oxygenation during cold storage has been established in islet isolation and transplantation to prevent ischemic tissue damage using perfluorodecalin (PFD) as hyperoxygen carrier. However, studies in humans and pigs provided conflicting results about the efficiency of PFD for pancreas oxygenation. The aim of this study was to compare PFD with a newly developed oxygen carrier composed of perfluorohexyloctane and polydimethylsiloxane 5 (F6H8S5) for long-term storage of human pancreata.
  
  
  METHODS
  After 24-hr storage in preoxygenated PFD or F6H8S5, pancreata were processed using Liberase HI for pancreas dissociation and a Ficoll gradient for islet purification. Islet quality assessment was performed measuring glucose-stimulated insulin release, viability, islet ATP content, and posttransplant function in diabetic nude mice.
  
  
  RESULTS
  Compared with PFD, F6H8S5 significantly increased the intrapancreatic partial oxygen pressure and islet ATP content. This corresponded to an increase of islet yield, recovery after culture, glucose stimulation index, viability, and improved graft function in diabetic nude mice.
  
  
  CONCLUSIONS
  The present findings indicate clearly that F6H8S5 improves isolation outcome after prolonged ischemia compared with PFD. This observation seems to be related to the significant lipophilicity and almost pancreas-specific density of F6H8S5. Moreover, these characteristics facilitate pancreas shipment without using custom-made transport vessels as required for PFD.
authors.affiliation: Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden. Daniel.Brandhorst@klinimm.uu.se
authors.forename: Heide
authors.initials: H
authors.lastname: Brandhorst
authors.forename: Sana
authors.initials: S
authors.lastname: Asif
authors.forename: Karin
authors.initials: K
authors.lastname: Andersson
authors.forename: Bastian
authors.initials: B
authors.lastname: Theisinger
authors.forename: Helene H
authors.initials: HH
authors.lastname: Andersson
authors.forename: Maria
authors.initials: M
authors.lastname: Felldin
authors.forename: Aksel
authors.initials: A
authors.lastname: Foss
authors.forename: Kaija
authors.initials: K
authors.lastname: Salmela
authors.forename: Annika
authors.initials: A
authors.lastname: Tibell
authors.forename: Gunnar
authors.initials: G
authors.lastname: Tufveson
authors.forename: Olle
authors.initials: O
authors.lastname: Korsgren
authors.forename: Daniel
authors.initials: D
authors.lastname: Brandhorst
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D005466:Fluorocarbons / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D010100:Oxygen / Q000032:analysis; D010179:Pancreas; D013552:Swine; D014019:Tissue Donors
chemical_list: D001802:Blood Substitutes; D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; C008805:perfluorodecalin; D010100:Oxygen