Planar polarization of node cells determines the rotational axis of node cilia.
Rotational movement of the node cilia generates a leftward fluid flow in the mouse embryo because the cilia are posteriorly tilted. However, it is not known how anterior-posterior information is translated into the posterior tilt of the node cilia. Here, we show that the basal body of node cilia is initially positioned centrally but then gradually shifts toward the posterior side of the node cells. Positioning of the basal body and unidirectional flow were found to be impaired in compound mutant mice lacking Dvl genes. Whereas the basal body was normally positioned in the node cells of Wnt3a(-/-) embryos, inhibition of Rac1, a component of the noncanonical Wnt signalling pathway, impaired the polarized localization of the basal body in wild-type embryos. Dvl2 and Dvl3 proteins were found to be localized to the apical side of the node cells, and their location was polarized to the posterior side of the cells before the posterior positioning of the basal body. These results suggest that posterior positioning of the basal body, which provides the posterior tilt to node cilia, is determined by planar polarization mediated by noncanonical Wnt signalling.
Hashimoto
Masakazu
M
Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Shinohara
Kyosuke
K
Wang
Jianbo
J
Ikeuchi
Shingo
S
Yoshiba
Satoko
S
Meno
Chikara
C
Nonaka
Shigenori
S
Takada
Shinji
S
Hatta
Kohei
K
Wynshaw-Boris
Anthony
A
Hamada
Hiroshi
H
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D002923:Cilia / Q000378:metabolism*; D000072261:Dishevelled Proteins; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins; D051379:Mice; D008817:Mice, Mutant Strains; D018613:Microscopy, Confocal; D009479:Neuropeptides / Q000037:antagonists & inhibitors / Q000502:physiology; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D060508:Wnt3 Protein; D060509:Wnt3A Protein; D020744:rac GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D020830:rac1 GTP-Binding Protein
D048868:Adaptor Proteins, Signal Transducing; D000634:Aminoquinolines; D000072261:Dishevelled Proteins; C000606582:Dvl2 protein, mouse; C000606585:Dvl3 protein, mouse; C487513:NSC 23766; D009479:Neuropeptides; D010750:Phosphoproteins; D011743:Pyrimidines; C484602:Rac1 protein, mouse; D051153:Wnt Proteins; D060508:Wnt3 Protein; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein