title: Investigation on possible transformations of cortisol, cortisone and cortisol glucuronide in bovine faecal matter using liquid chromatography-mass spectrometry.
abstract: Given the close resemblance of the ring A structure of prednisolone and prednisone on the one hand, and of androstadienedione on the other, the transformation of cortisol and cortisone into prednisolone and prednisone in cattle faeces was evaluated. A simple method that does not involve extraction but only the 1:100 dilution of cattle faeces, spiking with 400ng/mL cortisol, cortisone or cortisol glucuronide and incubation of the suspension, was used. The analyses were performed by HPLC-MS(3) to detect the supposed Delta(1) dehydrogenation of the glucocorticoids. The decision limits (CCalpha) and detection capabilities (CCbeta) were 2.0 and 3.0ng/mL for cortisol, cortisone and prednisolone, 3.0 and 4.0ng/mL for cortisol glucuronide and 7.0 and 10.0ng/mL for prednisone, respectively. Intra-day and inter-day coefficients of variation (CV%), were 5.6-6.2 and 5.2-6.6 for cortisol glucuronide, cortisol, cortisone and prednisolone, and 16.0 and 16.2 for prednisone, respectively. The recoveries were in the range 110-143% for all analytes. Regression coefficients (R2) were in the range 0.996-0.999 for all analytes. The results show the hydrolysis of the conjugated form and the dehydrogenation in ring A in diluted faeces. It is therefore predicted that urine contaminated with faeces may be positive for prednisone and prednisolone in the same way as they are positive for boldenone, i.e. as a result of microbiological dehydrogenase activity on cortisol and cortisone.
authors.affiliation: Department of Veterinary Sciences and Technologies for Food Safety, University of Milan, Via Celoria 10, 20133 Milan, Italy. francesco.arioli@unimi.it <francesco.arioli@unimi.it>
authors.forename: Francesco
authors.initials: F
authors.lastname: Arioli
authors.forename: Marco
authors.initials: M
authors.lastname: Fidani
authors.forename: Alessio
authors.initials: A
authors.lastname: Casati
authors.forename: Maria L
authors.initials: ML
authors.lastname: Fracchiolla
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pompa
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D003348:Cortisone / Q000737:chemistry / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D020719:Glucuronides / Q000737:chemistry / Q000378:metabolism*; D006854:Hydrocortisone / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D020719:Glucuronides; D003348:Cortisone; D006854:Hydrocortisone