Characterization of anti-Coxsackie virus B3 constituents of Radix Astragali by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
To profile the anti-Coxsackie virus B3 constituents of Radix Astragali, an HPLC-DAD-MS(n) analytical method, combined with an in vivo test, has been developed to identify the constituents of the active part, which has been demonstrated to have potency to inhibit the proliferation of virus in cardiac muscle, alleviate infraction in heart and elevate the survival rate of the animal. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 19 compounds, including 11 isoflavonoids and eight saponins, were identified, among which one pterocarpane glucoside was reported for the first time. The present study provides an approach to rapidly screening bioactive constituents in traditional Chinese medicines.
Ye
Guan
G
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China. yg4847@yahoo.com.cn
Tang
Yi-Hong
YH
Xia
Guang-Xin
GX
Sun
Zhao-Lin
ZL
Li
Zhi-Xiong
ZX
Huang
Cheng-Gang
CG
D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003384:Coxsackievirus Infections / Q000188:drug therapy / Q000821:virology; D029822:Enterovirus B, Human / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000998:Antiviral Agents; D010936:Plant Extracts