SDS-PAGE and two-dimensional maps in a radial gel format.
A novel method for performing 2-D map analysis is here reported, consisting in a modification of the second dimension run, which is performed not in a conventional square- or rectangular-size gel, but in a radial surface. This has the advantage of permitting resolution of closely adjacent bands, representing strings of isoforms of similar or identical mass but of closely spaced isoelectric points. When used in a mono-dimensional, SDS-PAGE format, this system allows the simultaneous running of 62 sample tracks. Examples are given of separation of plasma and urinary proteins.
Millioni
Renato
R
Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy.
Miuzzo
Manuela
M
Antonioli
Paolo
P
Sbrignadello
Stefano
S
Iori
Elisabetta
E
Dosselli
Ryan
R
Puricelli
Lucia
L
Kolbe
Markus
M
Tessari
Paolo
P
Righetti
Pier Giorgio
PG
D001798:Blood Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels / Q000737:chemistry*; D006801:Humans; D007526:Isoelectric Point; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D013499:Surface Properties; D016482:Urinalysis
D001798:Blood Proteins; D005782:Gels; D011506:Proteins; D020543:Proteome