Activation of Leishmania (Leishmania) amazonensis arginase at low temperature by binuclear Mn2+ center formation of the immobilized enzyme on a Ni2+ resin.
In Leishmania, arginase is responsible for the production of ornithine, a precursor of polyamines required for proliferation of the parasite. In this work, the activation kinetics of immobilized arginase enzyme from L. (L.) amazonensis were studied by varying the concentration of Mn2+ applied to the nickel column at 23 degrees C. The intensity of the binding of the enzyme to the Ni2+ resin was directly proportional to the concentration of Mn2+. Conformational changes of the enzyme may occur when the enzyme interacts with immobilized Ni2+, allowing the following to occur: (1) entrance of Mn2+ and formation of the metal bridge; (2) stabilization and activation of the enzyme at 23 degrees C; and (3) an increase in the affinity of the enzyme to Ni2+ after the Mn2+ activation step. The conformational alterations can be summarized as follows: the interaction with the Ni2+ simulates thermal heating in the artificial activation by opening a channel for Mn2+ to enter.
Silva
Edson R
ER
Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, São Paulo, SP, Brazil. edsilva@usp.br
Floeter-Winter
Lucile M
LM
D001119:Arginase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000187:drug effects / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D007475:Ion Exchange Resins; D007894:Leishmania mexicana / Q000187:drug effects / Q000201:enzymology*; D017895:Manganese Compounds / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013431:Sulfates / Q000494:pharmacology
D004800:Enzymes, Immobilized; D007475:Ion Exchange Resins; D017895:Manganese Compounds; D011994:Recombinant Proteins; D013431:Sulfates; D009532:Nickel; D001119:Arginase; C039798:manganese sulfate