[Study on effect of enzyme linked immunosorbent assay kits of norovirus].
OBJECTIVE
Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.


METHODS
The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.


RESULTS
RT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.


CONCLUSIONS
RIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.
Zhang
Qing
Q
Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Jin
Miao
M
Cui
Shu-Xian
SX
Liu
Na
N
Li
Lei
L
Duan
Zhao-Jun
ZJ
D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000737:chemistry / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic