The use of amniotic membrane in the repair of duodenal wounds in Wistar rats.
OBJECTIVE
In the search of a new material to repair duodenal wounds, a trial was conducted to assess the behavior of human amniotic membrane in the repair of the duodenal wall in rats.


METHODS
Fifty Wistar rats weighing between 250 and 350g, male, were submitted to duodenotomy and randomly distributed into two groups. Group A (n=8) had no treatment and was used as the control group. In Group B (n=42) the duodenal wound was treated with a patch of human amniotic membrane.


RESULTS
All animals in Group A died. In Group B no changes were observed with regards to death or the formation of duodenal fistula. All animals presented peritoneal adherences in the region on the duodenal wall repair and intestinal obstruction was observed in two animals. Healing of the duodenal wall in the region of the patch took place progressively as the post-operatory period increased, with regeneration of the mucosa and of the smooth muscle layer.


CONCLUSIONS
From the clinical standpoint, the amniotic membrane proved to be a biological tissue which served as a temporary seal and allowed the wound to heal by second-intention, with re-establishment of the duodenal wall structure.
Schimidt
Luciano Rodrigues
LR
University Hospital, UFSC, SC, Brazil.
Cardoso
Edson José
EJ
Schimidt
Rogério Rodrigues
RR
Back
Luiz Augusto
LA
Schiazawa
Maria Beatriz
MB
d'Acampora
Armando José
AJ
Sousa
Monique Viana de
MV
D000650:Amnion / Q000637:transplantation*; D000818:Animals; D001684:Biological Dressings; D004386:Duodenum / Q000293:injuries / Q000601:surgery*; D006801:Humans; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology*