title: Expression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells.
abstract: Human colorectal cancer antigen GA733-2 fused to the immunoglobin Fc fragment (GA733-2-Fc) was expressed in stably transformed Drosophila melanogaster S2 cells, and the immunogenicity of recombinant GA733-2-Fc was investigated in mice. Recombinant GA733-2-Fc was secreted into a culture medium with a molecular mass of approximately 65 kDa. Recombinant GA733-2-Fc was purified to homogeneity using affinity fractionation with Protein A sepharose 4 Fast Flow. Recombinant GA733-2-Fc proteins elicited production of specific antibodies against recombinant GA733-2 by immunization through an intraperitoneal route. Recombinant GA733-2-Fc-induced antibodies showed a binding activity to human colorectal carcinoma HCT-116 cells. Secretory recombinant GA733-2-Fc from Drosophila S2 cell systems can be used as an effective experimental antigen for research in cancer vaccine development.
authors.affiliation: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Suwon 446-701, Korea.
authors.forename: Yeon Ju
authors.initials: YJ
authors.lastname: Seok
authors.forename: Kyung Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Jeon
authors.initials: J
authors.lastname: Hwang-Bo
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Shon
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Ko
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kang
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Lee
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Joung
authors.forename: In Sik
authors.initials: IS
authors.lastname: Chung
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology*; D004331:Drosophila melanogaster / Q000166:cytology*; D004591:Electrophoresis, Polyacrylamide Gel; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006031:Glycosylation; D045325:HCT116 Cells; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014170:Transformation, Genetic
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D007141:Immunoglobulin Fc Fragments; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins