Secreted production of self-assembling peptides in Pichia pastoris by fusion to an artificial highly hydrophilic protein.
The undecapeptides CH(3)CO-Gln-Gln-Arg-Phe-Gln-Trp-Gln-Phe-Glu-Gln-Gln-NH(2) (P(11)-2) and CH(3)CO-Gln-Gln-Orn-Phe-Orn-Trp-Orn-Phe-Orn-Gln-Gln-NH(2) (P(11)-14) have unique self-assembly characteristics and broad application potential. Originally, these peptides were produced by chemical synthesis, which is costly and difficult to scale up to industrial levels in an economically feasible way. This article describes the efficient secreted production of these peptides (with free termini and ornithines replaced with lysines) in the methylotrophic yeast Pichia pastoris. The peptides were produced as enterokinase-cleavable fusions to the C-terminus of an artificial Solubility-Enhancing Protein (SEP). In vitro, the fused highly hydrophilic SEP proved to prevent self-assembly of the peptides. The SEP domain also facilitates product detection and allows convenient separation of the fusion protein from the broth by simple salt precipitation. After cleavage of the purified fusion protein with enterokinase, the free undecapeptides were obtained and P(11)-2 spontaneously assembled into a self-supporting gel, as intended. The properties of the SEP carrier could be advantageous for the production of other peptides.
Moers
Antoine P H A
AP
Biobased Products, Agrotechnology & Food Sciences Group, Wageningen UR, NL-6708 WG Wageningen, The Netherlands.
Wolbert
Emil J H
EJ
de Wolf
Frits A
FA
Werten
Marc W T
MW
D002851:Chromatography, High Pressure Liquid; D004765:Enteropeptidase / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D016018:Least-Squares Analysis; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides; D011993:Recombinant Fusion Proteins; D004765:Enteropeptidase