title: Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract.
abstract: The novel pandemic influenza H1N1 (H1N1pdm) virus of swine origin causes mild disease but occasionally leads to acute respiratory distress syndrome and death. It is important to understand the pathogenesis of this new disease in humans. We compared the virus tropism and host-responses elicited by pandemic H1N1pdm and seasonal H1N1 influenza viruses in ex vivo cultures of human conjunctiva, nasopharynx, bronchus, and lung, as well as in vitro cultures of human nasopharyngeal, bronchial, and alveolar epithelial cells. We found comparable replication and host-responses in seasonal and pandemic H1N1 viruses. However, pandemic H1N1pdm virus differs from seasonal H1N1 influenza virus in its ability to replicate in human conjunctiva, suggesting subtle differences in its receptor-binding profile and highlighting the potential role of the conjunctiva as an additional route of infection with H1N1pdm. A greater viral replication competence in bronchial epithelium at 33 degrees C may also contribute to the slight increase in virulence of the pandemic influenza virus. In contrast with highly pathogenic influenza H5N1 virus, pandemic H1N1pdm does not differ from seasonal influenza virus in its intrinsic capacity for cytokine dysregulation. Collectively, these results suggest that pandemic H1N1pdm virus differs in modest but subtle ways from seasonal H1N1 virus in its intrinsic virulence for humans, which is in accord with the epidemiology of the pandemic to date. These findings are therefore relevant for understanding transmission and therapy.
authors.affiliation: Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China. malik@hkucc.hku.hk.
authors.forename: Michael C W
authors.initials: MC
authors.lastname: Chan
authors.forename: Renee W Y
authors.initials: RW
authors.lastname: Chan
authors.forename: Wendy C L
authors.initials: WC
authors.lastname: Yu
authors.forename: Carol C C
authors.initials: CC
authors.lastname: Ho
authors.forename: Kit M
authors.initials: KM
authors.lastname: Yuen
authors.forename: Joanne H M
authors.initials: JH
authors.lastname: Fong
authors.forename: Lynsia L S
authors.initials: LL
authors.lastname: Tang
authors.forename: Wico W
authors.initials: WW
authors.lastname: Lai
authors.forename: Amy C Y
authors.initials: AC
authors.lastname: Lo
authors.forename: W H
authors.initials: WH
authors.lastname: Chui
authors.forename: Alan D L
authors.initials: AD
authors.lastname: Sihoe
authors.forename: Dora L W
authors.initials: DL
authors.lastname: Kwong
authors.forename: David S H
authors.initials: DS
authors.lastname: Wong
authors.forename: George S W
authors.initials: GS
authors.lastname: Tsao
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: John M
authors.initials: JM
authors.lastname: Nicholls
authors.forename: Joseph S M
authors.initials: JS
authors.lastname: Peiris
mesh_terms: D056809:Alveolar Epithelial Cells / Q000378:metabolism; D000818:Animals; D001980:Bronchi / Q000166:cytology; D003228:Conjunctiva / Q000821:virology*; D016207:Cytokines / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism*; D007251:Influenza, Human / Q000821:virology*; D009975:Orthomyxoviridae / Q000378:metabolism; D058873:Pandemics; D012137:Respiratory System / Q000821:virology*; D012621:Seasons; D013045:Species Specificity
chemical_list: D016207:Cytokines