Regulation effects on abnormal glucose and lipid metabolism of TZQ-F, a new kind of Traditional Chinese Medicine.
OBJECTIVE
Based on the recipe of the traditional anti-diabetic formula TZQ, we developed TZQ-F, a new formula including 8 fractions isolated from Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf with a good quality assurance. The study was aimed at fraction preparation and effects of the fractions on abnormal glucose and lipid metabolism.


METHODS
The active fractions were obtained by macroporous resin, ion-exchange resin and polyamide resin column chromatographies. HPLC analyses were used for quality control. In vitro mechanism study included DPPH radical scavenging, AGEs formation inhibition, alpha-glucosidase inhibition and lipase inhibition, and rats on high-fat diet were used for in vivo study.


RESULTS
In vitro mechanism study showed that among the 8 fractions, three of them had inhibition effects on intestinal disaccharase, three with inhibition effects on lipase, and five with effects of free radical scavenging. In vivo study showed that after 4 weeks of treatment, TZQ-F significantly decreased the levels of serum total cholesterol, TG, glucose, LDL-C and HDL-C in rats on high-fat diet. Consistent with the in vitro and in vivo results, histology study demonstrated that TZQ-F alleviated hepatic steatosis induced by high-fat diet.


CONCLUSIONS
TZQ-F possesses the potential regulation effects on abnormal glucose and lipid metabolism.
Tao
Wang
W
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, #312 Anshanxi Road, Nankai District, Tianjin, 300193, China.
Deqin
Zhang
Z
Yuhong
Li
L
Hong
Liu
L
Zhanbiao
Liu
L
Chunfeng
Zhao
Z
Limin
Hu
H
Xiumei
Gao
G
D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use; D008078:Cholesterol, LDL / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal; D005234:Fatty Liver / Q000188:drug therapy; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D005947:Glucose / Q000494:pharmacology / Q000627:therapeutic use; D044882:Glucose Metabolism Disorders; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D031625:Morus / Q000378:metabolism; D029596:Paeonia / Q000378:metabolism; D010618:Phenanthrolines; D018515:Plant Leaves / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D000520:alpha-Glucosidases / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use
D008078:Cholesterol, LDL; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010618:Phenanthrolines; C028972:dan-shen root extract; D002784:Cholesterol; D008049:Lipase; D000520:alpha-Glucosidases; D005947:Glucose