title: Immunohistochemical organization patterns of the follicular dendritic cells, myofibroblasts and macrophages in the human spleen--new considerations on the pathological diagnosis of splenectomy pieces.
abstract: There is reliable information about how changes in spleen histology are influenced by the relationship among B and T lymphocytes, macrophages, dendritic cells and myofibroblasts. Moreover, if it can be applied in the day-by-day pathology laboratory. This work intends to elucidate morpho-functional aspects of relationships of these cells in the different spleen compartments, how they are influenced by pathological conditions and how basic immunohistochemical techniques could optimize the histopathological diagnosis. We analyzed the usefulness of the monoclonal antibodies CD45RO, CD20, CD21, CD35, CD68, caldesmon, the smooth muscle alpha-actin type 1 (SMA-1) in 91 specimens. CD21(+) CD35(+) follicular dendritic cells were organized into three patterns in agreement with the immune condition of the lymphoid follicle. Smooth muscle alpha-actin type 1(+)and caldesmon(+)myofibroblasts draw two double rings: marginal-perifollicular and germinal-marginal. The latter is closely related to T-cells. CD68(+)red pulp macrophages had clear and linear configuration. The interruption of this CD68(+) linear pattern in splenic marginal zone lymphoma cases could be a criterion to differentiate it from reactive hyperplasia. CD45RO, CD20, CD21, CD68 and SMA-1 provide a basic and quality immunohistochemical battery for a better comprehension of the human spleen and could improve its histopathological diagnosis.
authors.affiliation: University Hospital Ramón y Cajal. Colmenar Viejo Road, km. 9,100. Internal Medicine Department. Zip code: 28034, Madrid, Spain. pg_vasco@yahoo.es
authors.forename: Pablo
authors.initials: P
authors.lastname: Guisado Vasco
authors.forename: José L
authors.initials: JL
authors.lastname: Villar Rodríguez
authors.forename: José
authors.initials: J
authors.lastname: Ibañez Martínez
authors.forename: Ricardo
authors.initials: R
authors.lastname: González Cámpora
authors.forename: Hugo
authors.initials: H
authors.lastname: Galera Davidson
mesh_terms: D020566:Dendritic Cells, Follicular / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000601:surgery; D013156:Splenectomy