Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.
The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6(4)22 or P6(2)22 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 A.
Higashi
Yasuhiro
Y
Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA. yhigashi@danforthcenter.org
Smith
Thomas J
TJ
Jez
Joseph M
JM
Kutchan
Toni M
TM
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003460:Crystallization; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D010207:Papaver / Q000201:enzymology*; D014961:X-Ray Diffraction
D010088:Oxidoreductases; C520720:salutaridine reductase, Papaver