title: Eradication of Helicobacter pylori increases ghrelin mRNA expression in the gastric mucosa.
abstract: It has been suggested that Helicobacter pylori eradication may influence production of some peptides in the stomach, which can affect appetite. This hypothesis is controversial. To verify the hypothesis, we conducted this randomized controlled trial using H. pylori infected subjects without any gastrointestinal symptoms. The treatment group received triple H. pylori eradication therapy for 7 days and the control group received no medication. We measured ghrelin, obestatin and the tumor necrosis factor-alpha (TNF-alpha) mRNA levels in endoscopic biopsy specimens and the changes from baseline to follow-up. The plasma active n-octanoyl ghrelin and obestatin levels were measured in both groups. The ghrelin/obestatin ratios in plasma and gastric mRNA expression were calculated at baseline and follow-up. Ghrelin mRNA expression in the fundic mucosa after H. pylori eradication increased significantly compared to the control group (4.47+/-2.14 vs. 1.79+/-0.96, P=0.009), independent of inflammatory changes. However, obestatin mRNA expression decreased in the antral mucosa (-0.57+/-1.06 vs. 0.41+/-0.72, P=0.028). The treatment group showed a marginal increase (P=0.060) in plasma ghrelin/obestatin ratio. The TNF-alpha mRNA expression also decreased significantly with treatment. This randomized controlled trial demonstrates that H. pylori eradication increases ghrelin mRNA expression, independent of inflammatory cell changes.
authors.affiliation: Department of Family Medicine, Center for Health Promotion and Clinical Research Center, Ilsan-Paik Hospital, College of Medicine, Inje University, Goyang, Korea.
authors.forename: Eon Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Yeong Sook
authors.initials: YS
authors.lastname: Yoon
authors.forename: Cheol-Young
authors.initials: CY
authors.lastname: Park
authors.forename: Han-Seong
authors.initials: HS
authors.lastname: Kim
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Um
authors.forename: Hyun Wook
authors.initials: HW
authors.lastname: Baik
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Jang
authors.forename: Sangyeoup
authors.initials: S
authors.lastname: Lee
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Park
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Oh
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D005773:Gastroscopy; D054439:Ghrelin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D054439:Ghrelin; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha