title: Preparation and characteristics of interferon-alpha poly(lactic-co-glycolic acid) microspheres.
abstract: By a double emulsion solvent evaporation method, interferon-alpha (IFN-alpha) microspheres were prepared with poly(lactide-co-glycolide) (PLGA) and their characteristics, such as morphology, drug loading, encapsulation efficiency, in vitro release and degradation were evaluated. The IFN-alpha microspheres were prepared by different viscosities from 0.17-1.13 dL g(-1) and concentrations between 5-25% of PLGA, which not only affected the drug loading and encapsulation efficiency of IFN-alpha microspheres, but also strongly influenced the in vitro release. With smooth and porous surface, the drug loading and encapsulation efficiency of the microspheres prepared by 15% 0.89 dL g(-1) PLGA were 7.736% and 77.38%, respectively. The DSC curve of microspheres indicated IFN-alpha was loaded inside the microspheres. The degradation of microspheres was homogeneous and the mass loss was over 80% in 6 weeks. The release profile of microspheres showed a sustained fashion and the IFN-alpha released from microspheres maintained its bioactivity for 7 days.
authors.affiliation: Department of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Feng-Lan
authors.initials: FL
authors.lastname: Song
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Pan
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Wang
authors.forename: Yi-qun
authors.initials: YQ
authors.lastname: Yang
authors.forename: Yao-Ming
authors.initials: YM
authors.lastname: Zhao
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Liang
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D014783:Viscosity
chemical_list: D003692:Delayed-Action Preparations; D016898:Interferon-alpha; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid