title: Protein-protein-interactions in a multiplexed, miniaturized format a functional analysis of Rho GTPase activation and inhibition.
abstract: A miniaturized, bead-based protein-protein-interaction assay was developed to study the interaction of Rho GTPases with regulatory proteins. The setup, which uses only minute amounts of sample, was used to analyze small molecules that inhibit the interaction between Rho GTPases and RhoGDI alpha. Prenylcysteine analogues and the replacement of GDP by non-hydrolysable GTP analogues prevented the formation of Rho GTPase-RhoGDI alpha complexes in a concentration-dependent manner.
authors.affiliation: NMI Natural and Medical Sciences Institute, University of Tuebingen, Reutlingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmohl
authors.forename: Stefanie
authors.initials: S
authors.lastname: Rimmele
authors.forename: Oliver
authors.initials: O
authors.lastname: Pötz
authors.forename: Yoel
authors.initials: Y
authors.lastname: Kloog
authors.forename: Peter
authors.initials: P
authors.lastname: Gierschik
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Joos
authors.forename: Nicole
authors.initials: N
authors.lastname: Schneiderhan-Marra
mesh_terms: D004789:Enzyme Activation; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D020741:rho GTP-Binding Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
chemical_list: D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D020741:rho GTP-Binding Proteins