title: High-performance liquid chromatographic method for the separation of enantiomeric gatifloxacin.
abstract: A high-performance liquid chromatographic method has been developed in normal-phase conditions for the separation of enantiomeric gatifloxacin, (+/-) 1-cyclopropyl-6-fluoro-8-methoxy-7-(3-methylpiperazin-1-yl)-4-oxo-quinoline-3-carboxylic acid, an antibiotic in bulk drug. The method involved the use of an amylose-based Chiralpak AD-H (150 mm x 4.6 mm, 5 microm) column using a mobile phase system containing n-hexane-ethanol-diethylamine (85:15:0.1% v/v). The conditions affording the best resolution were found by selection and variation of the mobile-phase compositions and the differences in separation capability of the method is noted. Relative standard deviation of retention times and peak areas were better than 0.2% and 0.4%, respectively, for precision. Gatifloxacin sample solution and mobile phase are found to be stable for at least 48 h.
authors.affiliation: Suven Life Sciences Limited, Serene Chambers, Road 5, Avenue 7, Banjara Hills, Hyderabad - 500034, India. amakrishna_nirogi@yahoo.co.in
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nirogi
authors.forename: Srinivasulu
authors.initials: S
authors.lastname: Kota
authors.forename: Saritha
authors.initials: S
authors.lastname: Vennila
authors.forename: Bujjibabu
authors.initials: B
authors.lastname: Lingavarapu
authors.forename: Vishwottam
authors.initials: V
authors.lastname: Kandikere
authors.forename: Koteshwara
authors.initials: K
authors.lastname: Mudigonda
authors.forename: Hima Bindu
authors.initials: HB
authors.lastname: Vurimindi
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D024841:Fluoroquinolones / Q000737:chemistry / Q000302:isolation & purification*; D000077734:Gatifloxacin; D057230:Limit of Detection; D048448:Phenylcarbamates / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D048448:Phenylcarbamates; C080033:Chiralpak AD; D000688:Amylose; D000077734:Gatifloxacin