title: The qualities of cryopreserved epididymal sperm collected from feline epididymides stored at low temperature.
abstract: We observed the influences of low-temperature storage of the feline epididymis on the epididymal semen qualities before and after cryopreservation to identify the optimal duration for low-temperature storage of the epididymis. After excision, the feline epididymis was stored at 4 degrees C for 0-72 hr and then subjected to epididymal sperm collection. When sperm from the refrigerated cauda epididymis were frozen and thawed, there was no significant difference in sperm motility between the 0- and 24-hr low-temperature storage groups, but sperm motility was significantly decreased in the 48-hr storage group. The above findings suggested that low-temperature storage of the epididymis until 24 hr is useful for frozen sperm collected from the feline cauda epididymis.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, Japan. marimaru@sd5.so-net.ne.jp
authors.forename: Mari
authors.initials: M
authors.lastname: Toyonaga
authors.forename: Yuuka
authors.initials: Y
authors.lastname: Sato
authors.forename: Miki
authors.initials: M
authors.lastname: Morita
authors.forename: Masashi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Oba
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Mizutani
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D002415:Cats; D002470:Cell Survival; D003080:Cold Temperature; D015925:Cryopreservation / Q000662:veterinary*; D056727:Endangered Species; D004822:Epididymis / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013079:Sperm Maturation / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000166:cytology / Q000502:physiology; D013737:Testis / Q000502:physiology