Low-cost fluorimetric determination of radicals based on fluorogenic dimerization of the natural phenol sesamol.
A novel fluorimetric method for determining radicals using the natural phenol sesamol as a fluorogenic reagent is reported. In this assay, sesamol was reacted with aqueous radicals to yield one isomer of a sesamol dimer exclusively. The dimer emitted purple fluorescence near 400 nm around neutral pH, where it assumed the monoanionic form. This method was applied to the straightforward detection of radical nitric oxide (NO). The ready availability of sesamol should enable rapid implementation of applications utilizing this new assay, particularly in high-throughput analysis or screening.
Makino
Yumi
Y
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Uchiyama
Seiichi
S
Ohno
Ken-ichi
K
Arakawa
Hidetoshi
H
D000042:Absorption; D000840:Anisoles / Q000737:chemistry; D052117:Benzodioxoles / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry*; D002021:Buffers; D019281:Dimerization; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000191:economics* / Q000379:methods*; D005609:Free Radicals / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D009569:Nitric Oxide / Q000032:analysis / Q000737:chemistry; D010087:Oxides / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D000840:Anisoles; D052117:Benzodioxoles; D001688:Biological Products; D002021:Buffers; D005456:Fluorescent Dyes; D005609:Free Radicals; D010087:Oxides; D010636:Phenols; D012997:Solvents; D014867:Water; C052893:tert-butoxy; D009569:Nitric Oxide; C025583:sesamol; C060998:anisole