title: MiRNA expression profile of human subcutaneous adipose and during adipocyte differentiation.
abstract: BACKGROUND
  Potential regulators of adipogenesis include microRNAs (miRNAs), small non-coding RNAs that have been recently shown related to adiposity and differentially expressed in fat depots. However, to date no study is available, to our knowledge, regarding miRNAs expression profile during human adipogenesis. Thereby, the aim of this study was to investigate whether miRNA pattern in human fat cells and subcutaneous adipose tissue is associated to obesity and co-morbidities and whether miRNA expression profile in adipocytes is linked to adipogenesis.
  
  
  RESULTS
  We performed a global miRNA expression microarray of 723 human and 76 viral mature miRNAs in human adipocytes during differentiation and in subcutaneous fat samples from non-obese (n = 6) and obese with (n = 9) and without (n = 13) Type-2 Diabetes Mellitus (DM-2) women. Changes in adipogenesis-related miRNAs were then validated by RT-PCR. Fifty of 799 miRNAs (6.2%) significantly differed between fat cells from lean and obese subjects. Seventy miRNAs (8.8%) were highly and significantly up or down-regulated in mature adipocytes as compared to pre-adipocytes. Otherwise, 17 of these 799 miRNAs (2.1%) were correlated with anthropometrical (BMI) and/or metabolic (fasting glucose and/or triglycerides) parameters. We identified 11 miRNAs (1.4%) significantly deregulated in subcutaneous fat from obese subjects with and without DM-2. Interestingly, most of these changes were associated with miRNAs also significantly deregulated during adipocyte differentiation.
  
  
  CONCLUSIONS
  The remarkable inverse miRNA profile revealed for human pre-adipocytes and mature adipocytes hints at a closely crosstalk between miRNAs and adipogenesis. Such candidates may represent biomarkers and therapeutic targets for obesity and obesity-related complications.
authors.affiliation: Service of Diabetes, Endocrinology and Nutrition, Institut d'Investigació Biomédica de Girona, CIBEROBN (CB06/03/0010), Girona, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ortega
authors.forename: José M
authors.initials: JM
authors.lastname: Moreno-Navarrete
authors.forename: Gerard
authors.initials: G
authors.lastname: Pardo
authors.forename: Monica
authors.initials: M
authors.lastname: Sabater
authors.forename: Manuela
authors.initials: M
authors.lastname: Hummel
authors.forename: Anna
authors.initials: A
authors.lastname: Ferrer
authors.forename: Jose I
authors.initials: JI
authors.lastname: Rodriguez-Hermosa
authors.forename: Bartomeu
authors.initials: B
authors.lastname: Ruiz
authors.forename: Wifredo
authors.initials: W
authors.lastname: Ricart
authors.forename: Belen
authors.initials: B
authors.lastname: Peral
authors.forename: José M
authors.initials: JM
authors.lastname: Fernández-Real
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000235:genetics; D005215:Fasting / Q000097:blood; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000150:complications / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D035683:MicroRNAs; D014280:Triglycerides