title: Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide).
abstract: We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. With this method, the conformation of long genomic DNA can be readily and reversibly switched between a very compact condensate and an unfolded macromolecule.
authors.affiliation: Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Anatoly A
authors.initials: AA
authors.lastname: Zinchenko
authors.forename: Satoru
authors.initials: S
authors.lastname: Kidoaki
authors.forename: Shizuaki
authors.initials: S
authors.lastname: Murata
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D017122:Bacteriophage T4; D004247:DNA / Q000737:chemistry*; D016678:Genome / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D012996:Solutions; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D012996:Solutions; C052970:poly-N-isopropylacrylamide; D004247:DNA