Toona sinensis extracts induced cell cycle arrest and apoptosis in the human lung large cell carcinoma.
Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze-drying the supernatant of centrifuged crude extract from Toona sinensis leaves (TSL-1) to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3-(4-,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL-1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin-dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL-1 induced H661 cell apoptosis. Western blot analysis showed that TSL-1 reduced the expression of the anti-apoptotic protein B-cell lymphoma 2, and degraded the DNA repair protein, poly(ADP-ribose) polymerase. TSL-1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.
Wang
Cheng-Yuan
CY
Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
Lin
Kai-Huang
KH
Yang
Chih-Jen
CJ
Tsai
Jong-Rung
JR
Hung
Jen-Yu
JY
Wang
Pei-Hui
PH
Hsu
Hseng-Kuang
HK
Huang
Ming-Shyan
MS
D017209:Apoptosis / Q000187:drug effects*; D018287:Carcinoma, Large Cell / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
D018797:Cell Cycle Proteins; D010936:Plant Extracts