title: Evaluation of alpha-glucosidase, alpha-amylase and protein glycation inhibitory activities of edible plants.
abstract: The present study was to investigate in vitro alpha-glucosidase, pancreatic alpha-amylase and protein glycation inhibitory activities of nine edible plants. The results indicated that total phenolics, flavonoids, and condensed tannins of nine edible plants showed marked variations, ranging from 12.2 to 80.1 mg gallic acid equivalent/g extract, 2.34 to 13.65 mg quercetin equivalent/g extract, and 97.2 to 460.1 mg catechin equivalent/g extract, respectively. Our findings showed that grape seed, Cat's whiskers and Sweetleaf extract were the most effective pancreatic alpha-amylase, intestinal maltase, and sucrase inhibitor with IC(50) values of 0.29 +/- 0.01 mg/ml, 0.97 +/- 0.10 mg/ml and 0.86 +/- 0.01 mg/ml, respectively. All extracts (1 mg/ml) markedly inhibited the glycation of bovine serum albumin in fructose-mediated non-enzyme glycation by 50-30% at week 1. It was found that Pennywort maintained the high percentage inhibition among those of the extracts during the 4 weeks of experiment. These edible plants may be used for controlling blood glucose level and prevention of the development of type 2 diabetes.
authors.affiliation: The Medical Food Research and Development Center, Department of Transfusion Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand. Sirichai.a@chula.ac.th
authors.forename: Sirichai
authors.initials: S
authors.lastname: Adisakwattana
authors.forename: Pariwat
authors.initials: P
authors.lastname: Jiphimai
authors.forename: Pornsawan
authors.initials: P
authors.lastname: Prutanopajai
authors.forename: Benjanut
authors.initials: B
authors.lastname: Chanathong
authors.forename: Suwimol
authors.initials: S
authors.lastname: Sapwarobol
authors.forename: Tipayanate
authors.initials: T
authors.lastname: Ariyapitipan
mesh_terms: D000818:Animals; D002417:Cattle; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D065089:Glycoside Hydrolase Inhibitors; D006031:Glycosylation; D007004:Hypoglycemic Agents / Q000032:analysis / Q000494:pharmacology*; D007422:Intestines / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010945:Plants, Edible / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism; D013393:Sucrase / Q000037:antagonists & inhibitors; D013552:Swine; D013634:Tannins / Q000032:analysis / Q000494:pharmacology; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D005419:Flavonoids; D017127:Glycation End Products, Advanced; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010636:Phenols; D010936:Plant Extracts; D011506:Proteins; D012709:Serum Albumin; D013634:Tannins; D000516:alpha-Amylases; D013393:Sucrase