Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum.
Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM approximately = 1 g l(-1)) where the bacterium was grown for 24 h, at 30 degrees C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg(-1)). The growing cells produced 0.076 g ADD from 1 g cholesterol l(-1).
Chaudhari
Priti N
PN
School of Life Sciences, North Maharashtra University, P B 80, Jalgaon, 425 001, India.
Chaudhari
Bhushan L
BL
Chincholkar
Sudhir B
SB
D000332:Aerobiosis; D000730:Androstadienes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism; D045247:Chryseobacterium / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D010084:Oxidation-Reduction; D013696:Temperature; D013997:Time Factors
D000730:Androstadienes; D001426:Bacterial Proteins; D003470:Culture Media; C006573:1,4-androstadiene-3,17-dione; D002784:Cholesterol; D002789:Cholesterol Oxidase