title: Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis.
abstract: OBJECTIVE
  To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up).
  
  
  METHODS
  Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.
  
  
  RESULTS
  Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.
  
  
  CONCLUSIONS
  We found differences in Gene Ontology terms between the two groups. These differences could potentially be employed to establish markers of fertility success and to identify cellular processes and complex systems related with male infertility.
authors.affiliation: Instituto Universitario IVI Valencia, Universidad de Valencia, Valencia, Spain. sherrero@ivi.es
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Herrero
authors.forename: Nicolás
authors.initials: N
authors.lastname: Garrido
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Martínez-Conejero
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.forename: Marcos
authors.initials: M
authors.lastname: Meseguer
mesh_terms: D000328:Adult; D019295:Computational Biology; D016208:Databases, Factual; D020869:Gene Expression Profiling; D005796:Genes; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D055101:Semen Analysis / Q000379:methods; D012984:Software; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014019:Tissue Donors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger