Vitreous levels of interleukine-8 and monocyte chemoattractant protein-1 in macular oedema with branch retinal vein occlusion.
OBJECTIVE
To investigate whether interleukine-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are related with macular oedema in patients with branch retinal vein occlusions (BRVOs).


METHODS
Retrospective case-control study.


METHODS
Nineteen patients who had macular oedema due to BRVO and nine patients with non-ischaemic ocular diseases (control group).


METHODS
Macular oedema was examined by optical coherence tomography. Both venous blood and vitreous samples were obtained at the time of vitreoretinal surgery. IL-8 and MCP-1 levels in vitreous fluid and plasma were determined with enzyme-linked immunosorbent assay kits. Variables were compared with the Mann-Whitney U-test, Wilcoxon's signed-ranked test, and the chi2-test, when appropriate. To examine correlations, Spearman's rank-order correlation coefficients were calculated. Statistical significance was set at P<0.05.


RESULTS
The vitreous fluid levels of IL-8 (median: 63.5 pg/ml) and MCP-1 (median: 1522.4 pg/ml) were significantly higher in the patients with BRVO than in the control group (median: 5.1 and 746.5 pg/ml respectively; P<0.001 and <0.001 respectively). Vitreous IL-8 and MCP-1 were significantly correlated in patients with BRVO (P=0.009).


CONCLUSIONS
Both IL-8 and MCP-1 were elevated in the vitreous fluid of patients with BRVO and macular oedema. Both chemokines may contribute to the pathogenesis of macular oedema in patients with BRVO.
Fonollosa
A
A
Department of Ophthalmology, Hospital Valle Hebron de Barcelona, Barcelona, Spain. 36427afc@comb.es
Garcia-Arumi
J
J
Santos
E
E
Macia
C
C
Fernandez
P
P
Segura
R M
RM
Zapata
M A
MA
Rodriguez-Infante
R
R
Boixadera
A
A
Martinez-Castillo
V
V
D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D054426:Chemokine CXCL2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000378:metabolism*; D008269:Macular Edema / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012170:Retinal Vein Occlusion / Q000150:complications / Q000378:metabolism*; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence; D014822:Vitreous Body / Q000378:metabolism*
D015415:Biomarkers; D054426:Chemokine CXCL2; D016209:Interleukin-8