The influence of the environment on Cajal-Retzius cell migration.
During cerebral cortex development, different cell populations migrate tangentially through the preplate, traveling from their site of origin toward their final positions. One of the earliest populations formed, the Cajal-Retzius (C-R) cells, is mainly generated in different cortical hem (CH) domains, and they migrate along established and parallel routes to cover the whole cortical mantle. In this study, we present evidence that the phenotype of -Retzius cells, as well as some of their migratory characteristics, is specified in the area where the cells are generated. Nevertheless, when implanted ectopically, these cells can follow new migratory routes, indicating that locally provided genetic cues along the migratory path nonautonomously influence the position of these cells emanating from different portions of the CH. This was witnessed by performing CH implants of tissue expressing fluorescent tracers in live whole embryos. In the same way, tracer injections into the hem of Small eye mutant mice were particularly informative since the lack of Pax6 affects some guidance factors in the migratory environment. As a result, in these animals, the C-R cell population is disorganized, and it forms 1 day late, showing certain differences in gene expression that might help explain these disruptions.
Ceci
María Laura
ML
Instituto Cajal Consejo Superior de Investigaciones Científicas, Madrid 28002, Spain.
López-Mascaraque
Laura
L
de Carlos
Juan A
JA
D000818:Animals; D001578:Benzopyrans / Q000378:metabolism; D016380:Brain Tissue Transplantation / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology* / Q000637:transplantation; D004622:Embryo, Mammalian; D005136:Eye Proteins / Q000235:genetics; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000502:physiology*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics; D011247:Pregnancy; D012097:Repressor Proteins / Q000235:genetics
C505935:5-carboxyfluorescein diacetate acetoxymethyl ester; D001578:Benzopyrans; D005136:Eye Proteins; D005452:Fluoresceins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; C063917:SNARF dye; D049452:Green Fluorescent Proteins