E(nos)/CG4699 required for nanos function in the female germ line of Drosophila.
The translational repressor Nanos is required in the germ line stem cells of the Drosophila ovary to maintain their capacity for self-renewal. Following division of the stem cells, Nanos is inhibited in the daughters that differentiate into cysts and ultimately become mature oocytes. The control of Nanos activity is thus an important aspect of the switch from self-renewal to differentiation. In this report, we describe a genetic interaction between nanos and Enhancer of nos, an allele of the previously uncharacterized locus CG4699. We find that E(nos) protein is required for normal accumulation of Nanos in the ovary and thus for maintenance of the germ line. The mechanism by which E(nos)/CG4699 protein acts is not clear, although it has been found in a complex with Mof acetylase. Consistent with the finding that E(nos) interacts with Mof, we observe that nanos and mof also interact genetically to maintain normal oogenesis.
Yu
Lin
L
Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
Song
Yan
Y
Wharton
Robin P
RP
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015153:Blotting, Western; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic; D005260:Female; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D009154:Mutation; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D010063:Ovum / Q000166:cytology / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
C551525:CG4669 protein, Drosophila; D029721:Drosophila Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C070574:nos protein, Drosophila; D049452:Green Fluorescent Proteins