Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats.
OBJECTIVE
An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion.


METHODS
Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay.


RESULTS
In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2.


CONCLUSIONS
From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.
Jamel
Márcio José
MJ
Department of Surgery, Faculty of Medicine, University of Rio de Janeiro, Brazil.
Pereira
Lara de Paula Miranda
Lde P
Mello
Natália Bernardes
NB
Eleuthério
Elis Cristina Araújo
EC
Schanaider
Alberto
A
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004195:Disease Models, Animal; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood* / Q000175:diagnosis
D015415:Biomarkers; D001798:Blood Proteins