Observation of the solid-state photo-CIDNP effect in entire cells of cyanobacteria Synechocystis.
Cyanobacteria are widely used as model organism of oxygenic photosynthesis due to being the simplest photosynthetic organisms containing both photosystem I and II (PSI and PSII). Photochemically induced dynamic nuclear polarization (photo-CIDNP) (13)C magic-angle spinning (MAS) NMR is a powerful tool in understanding the photosynthesis machinery down to atomic level. Combined with selective isotope enrichment this technique has now opened the door to study primary charge separation in whole living cells. Here, we present the first photo-CIDNP observed in whole cells of the cyanobacterium Synechocystis.
Janssen
Geertje J
GJ
Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, The Netherlands.
Daviso
Eugenio
E
van Son
Martin
M
de Groot
Huub J M
HJ
Alia
A
A
Matysik
Jörg
J
D000622:Aminolevulinic Acid / Q000378:metabolism; D002247:Carbon Isotopes; D002853:Chromatography, Liquid; D007553:Isotope Labeling; D008027:Light; D055537:Light Signal Transduction / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055668:Photochemical Processes / Q000528:radiation effects*; D046939:Synechocystis / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*
D002247:Carbon Isotopes; D000622:Aminolevulinic Acid