Expression of transient receptor potential channel mucolipin (TRPML)  and polycystine (TRPP) in the mouse inner ear.
CONCLUSIONS
TRPML3 may play distinct roles in the inner ear, such as stereociliar organization, sensory cell transduction, and inner ear fluid homeostasis, and TRPP3 may be important for fluid homeostasis in the inner ear.


OBJECTIVE
To study the expression of TRPML1-3 and TRPP2, 3, and 5 in the mouse inner ear.


METHODS
Localization of TRPML1-3 and TRPP2, 3, and 5 in the inner ear of CBA/J mice was investigated by immunohistochemistry.


RESULTS
TRPML1-3 immunoreactivity was evident in the stria vascularis, spiral prominence, and spiral ligament. TRPML immunoreactivity was also observed in outer and inner hair cells, supporting cells, and spiral ganglion cells. The vestibular end organs, vestibular sensory cells, dark cells, and ganglion cells all showed immunoreactivity to TRPML. TRPP2 immunoreactivity was evident in the outer lining of the lateral wall of the cochlea, spiral ganglion cells, vestibular sensory cells, and ganglion cells. TRPP3 immunoreactivity was present in stria vascularis, spiral ganglion cells, vestibular sensory cells, dark cells, and ganglion cells. Faint TRPP5 immunoreactivity was observed in the spiral ganglion cells and vestibular ganglion cells.
Takumida
Masaya
M
Department of Otolaryngology, Hiroshima University Faculty of Medicine, Hiroshima 734-8551, Japan. masati@hiroshima-u.ac.jp
Anniko
Matti
M
D000483:Alleles; D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D003051:Cochlea / Q000166:cytology / Q000378:metabolism; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D017950:Ganglia, Sensory / Q000166:cytology / Q000378:metabolism; D006199:Hair Cells, Auditory, Inner / Q000166:cytology / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D020125:Mutation, Missense / Q000235:genetics; D009925:Organ of Corti / Q000166:cytology / Q000378:metabolism; D017354:Point Mutation / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D013136:Spiral Ganglion / Q000166:cytology / Q000378:metabolism; D013316:Stria Vascularis / Q000166:cytology / Q000378:metabolism; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism; D050396:TRPP Cation Channels / Q000235:genetics* / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism; D014725:Vestibular Nerve / Q000166:cytology / Q000378:metabolism
D015220:Calcium Channels; D011956:Receptors, Cell Surface; D050053:TRPM Cation Channels; D050396:TRPP Cation Channels; D050051:Transient Receptor Potential Channels; C510642:MCOLN3 protein, human; C512321:Mcoln1 protein, mouse; C117185:PKD2L1 protein, human