Isolation and characterization of oil palm constitutive promoter derived from ubiquitin extension protein (uep1) gene.
The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828 bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.
Masura
Subhi Siti
SS
Advanced Biotechnology and Breeding Centre, Biological Research Division, Malaysian Palm Oil Board, P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.
Parveez
Ghulam Kadir Ahmad
GK
Ismail
Ismanizan
I
D000040:Abscisic Acid / Q000494:pharmacology; D028023:Arecaceae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000378:metabolism; D007150:Immunohistochemistry; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D025801:Ubiquitin / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010938:Plant Oils; D025801:Ubiquitin; D000073878:Palm Oil; D000040:Abscisic Acid; D005966:Glucuronidase