title: 6,7-Difluoro-1,4-dihydro-1-methyl-4-oxo-3-quinolinecarboxylic acid, a newly designed fluorescence enhancement-type derivatizing reagent for amino compounds.
abstract: A novel fluorescence enhancement-type derivatizing reagent for amino compounds, 6,7-difluoro-1,4-dihydro-1-methyl-4-oxo-3-quinolinecarboxylic acid (FMQC), was developed. FMQC reacts with aliphatic primary amino compounds to afford strong fluorescent derivatives having high photo-and thermo-stabilities. The FMQC derivatives of amino compounds showed 12-159 times higher fluorescence quantum efficiencies than those of FMQC in aqueous and polar organic media. Additionally, the absorption and fluorescence emission wavelength of the derivatives are red-shifted from those of FMQC. These differences in the fluorescence properties between FMQC and the fluorescent derivative enabled the simple and highly sensitive determination of amino compounds without removing any excess unreacted FMQC by using a simple spectrofluorometer as well as HPLC.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Hirano
authors.forename: Kenji
authors.initials: K
authors.lastname: Hamase
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miyata
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Zaitsu
mesh_terms: D000042:Absorption; D000588:Amines / Q000737:chemistry*; D002138:Calibration; D002264:Carboxylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004048:Diethylamines / Q000737:chemistry; D005453:Fluorescence; D009025:Morpholines; D011437:Propylamines / Q000737:chemistry; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: C549937:6,7-difluoro-1,4-dihydro-1-methyl-4-oxo-3-quinolinecarboxylic acid; D000588:Amines; D002264:Carboxylic Acids; D004048:Diethylamines; D009025:Morpholines; D011437:Propylamines; D011804:Quinolines; D014867:Water; C020526:tiemonium methylsulfate; C034281:diethylamine