title: Slow magic-angle coil spinning: a high-sensitivity and high-resolution NMR strategy for microscopic biological specimens.
abstract: High-resolution magic-angle spinning has become a valuable tool to study biologic samples; however, there are only a few high-resolution magic-angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high-resolution (1)H NMR spectroscopic approach using magic-angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large-volume commercial magic-angle spinning rotor, where the microcoil offers incredible sensitivity and the large-volume magic-angle spinning rotor provides stable slow magic-angle spinning. This approach provides high-resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis.
authors.affiliation: Commissariat à l'Energie Atomique Saclay, IRAMIS, SIS2M/LSDRM, Gif-sur-Yvette, France.
authors.forename: Alan
authors.initials: A
authors.lastname: Wong
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Aguiar
authors.forename: Dimitris
authors.initials: D
authors.lastname: Sakellariou
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D008280:Magnetics / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers