10-Hydroxycamptothecin produced by a new endophytic Xylaria sp., M20, from Camptotheca acuminata.
A new 10-hydroxycamptothecin (HCPT)-producing fungus was isolated from Camptotheca acuminata. The strain was classified as a Xylaria sp. based on the internal transcribed spacer and 18S rDNA gene analysis. All elicitors tested, except methyl jasmonate, increased HCPT production in submerged culture. The maximum yield was 5.4 mg HCPT/l(-1), when salicylic acid was added at 0.1 mM to the culture medium.
Liu
Kaihui
K
School of Biological Sciences and Engineering, Shaanxi University of Technology, 723001, Hanzhong, Shaanxi, People's Republic of China. kaihhui168@yahoo.com.cn
Ding
Xiaowei
X
Deng
Baiwan
B
Chen
Wenqiang
W
D029769:Camptotheca / Q000382:microbiology*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013098:Xylariales / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*
D003470:Culture Media; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; C028098:10-hydroxycamptothecin; D002166:Camptothecin