title: Development and statistical validation of a guinea pig model for vaccine potency testing against Infectious Bovine Rhinothracheitis (IBR) virus.
abstract: Infectious Bovine Rhinothracheitis (IBR) caused by bovine herpesvirus 1 (BoHV-1) infection is distributed worldwide. BoHV-1 either alone or in association with other respiratory cattle pathogens causes significant economic losses to the livestock industry. The aim of this work was to validate a guinea pig model as an alternative method to the current BoHV-1 vaccine potency testing in calves. Guinea pigs were immunized with two doses of vaccine, 21 days apart and sampled at 30 days post vaccination (dpv). BoHV-1 antibody (Ab) response to vaccination in guinea pigs, measured by ELISA and virus neutralization (VN), was statistically compared to the Ab response in cattle. The guinea pig model showed a dose-response relationship to the BoVH-1 antigen concentration in the vaccine and it was able to discriminate among vaccines containing 1log(10) difference in its BoHV-1 concentration with very good repeatability and reproducibility (CV < or = 20%). A regression analysis of the Ab titers obtained in guinea pigs and bovines at 30 and 60dpv, respectively, allowed us to classify vaccines in three potency categories: "very satisfactory", "satisfactory" and "unsatisfactory". Bovines immunized with vaccines corresponding to each of these three categories were experimentally challenged with BoVH-1 virus, the level of protection, as measured by reduction of virus shedding and disease severity, correlated well with the vaccine category used. Data generated by 85 experiments, which included vaccination of calves and guinea pigs with 18 reference vaccines of known potency, 8 placebos and 18 commercial vaccines, was subjected to statistical analysis. Concordance analysis indicated almost perfect agreement between the model and the target species for Ab titers measured by ELISA and almost perfect to substantial agreement when Ab titers were measured by VN. Taken together these results indicate that the developed guinea pig model represents a novel and reliable tool to estimate batch-to-batch vaccine potency and to predict efficacy of killed BoHV-1 veterinary vaccines.
authors.affiliation: Instituto de Virología, CICV y A - INTA, Buenos Aires, Argentina. vparreno@cnia.inta.gov.ar
authors.forename: Viviana
authors.initials: V
authors.lastname: Parreño
authors.forename: María Virginia
authors.initials: MV
authors.lastname: López
authors.forename: Daniela
authors.initials: D
authors.lastname: Rodriguez
authors.forename: María Marta
authors.initials: MM
authors.lastname: Vena
authors.forename: Mercedes
authors.initials: M
authors.lastname: Izuel
authors.forename: Jorge
authors.initials: J
authors.lastname: Filippi
authors.forename: Alejandra
authors.initials: A
authors.lastname: Romera
authors.forename: Claudia
authors.initials: C
authors.lastname: Faverin
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Bellinzoni
authors.forename: Fernando
authors.initials: F
authors.lastname: Fernandez
authors.forename: Laura
authors.initials: L
authors.lastname: Marangunich
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D007242:Herpesvirus 1, Bovine / Q000276:immunology* / Q000472:pathogenicity*; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D009500:Neutralization Tests; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D014481:United States; D014765:Viral Vaccines / Q000276:immunology*; D017201:Virus Shedding / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014765:Viral Vaccines