title: Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats.
abstract: OBJECTIVE
  An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion.
  
  
  METHODS
  Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay.
  
  
  RESULTS
  In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2.
  
  
  CONCLUSIONS
  From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.
authors.affiliation: Department of Surgery, Faculty of Medicine, University of Rio de Janeiro, Brazil.
authors.forename: Márcio José
authors.initials: MJ
authors.lastname: Jamel
authors.forename: Lara de Paula Miranda
authors.initials: Lde P
authors.lastname: Pereira
authors.forename: Natália Bernardes
authors.initials: NB
authors.lastname: Mello
authors.forename: Elis Cristina Araújo
authors.initials: EC
authors.lastname: Eleuthério
authors.forename: Alberto
authors.initials: A
authors.lastname: Schanaider
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004195:Disease Models, Animal; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood* / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D001798:Blood Proteins