title: In vitro antibacterial activity of Phx-3 against Helicobacter pylori.
abstract: Phx-3, one of the phenoxazine derivatives, is reported to have inhibitory effect on Mycobacterium species and Chlamydia pneumoniae but not Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes. The bactericidal activities of Phx-3 against Helicobacter pylori strains have not been assessed. Then, we measured minimum inhibitory concentration of Phx-3 for Helicobacter strains and assessed the morphological and biochemical effects of Phx-3 on H. pylori. In present study, it has shown that H. pylori strains including clarithromycin resistant strain and Helicobacter musterae were killed effectively by the treatment with Phx-3. Furthermore, severe morphological changes such as membrane blebbing and formation of hollows in H. pylori were detected. In addition, induction of heat shock protein 60 was observed. Taken together, Phx-3 has antibacterial activity against Helicobacter pylori.
authors.affiliation: Department of Infectious Diseases, Division of Medical Microbiology, Kyorin University, School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan. thanawa@ks.kyorin-u.ac.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hanawa
authors.forename: Takako
authors.initials: T
authors.lastname: Osaki
authors.forename: Taki
authors.initials: T
authors.lastname: Manzoku
authors.forename: Minoru
authors.initials: M
authors.lastname: Fukuda
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.forename: Akio
authors.initials: A
authors.lastname: Tomoda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D010078:Oxazines / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010078:Oxazines; C005512:3-aminophenoxazone