The associations of hA3G and hA3B mRNA levels with HIV disease progression among HIV-infected individuals of China.
OBJECTIVE
To explore correlations between mRNA (hA3G, hA3F, and hA3B) levels and CD4 T-cell counts and HIV-1 viral loads to evaluate their respective roles in disease progression.


METHODS
Real-time polymerase chain reaction was used to quantify the mRNA levels of hA3G, hA3B, and hA3F in peripheral blood mononuclear cells from slow progress patients (SP), asymptomatic HIV-infected patients (AS), AIDS patients, and HIV-negative controls.


RESULTS
The levels of hA3G and hA3B mRNA correlated positively with CD4 T-cell counts (r = 0.436, P = 0.002, r = 0.334, P = 0.025), and negatively with HIV-1 viral loads (r = -0.306, P = 0.038, r = -0.301 P = 0.044). The levels of hA3G and hA3B mRNA in HIV-infected subjects were lower than in HIV-negative controls (P < 0.05), and hA3G and hA3B mRNA levels were significantly higher in SP than in AIDS patients (P < 0.05). There was no correlation between the hA3F mRNA level and CD4 T-cell counts or between the hA3F mRNA level and HIV-1 viral loads.


CONCLUSIONS
Higher expression levels of hA3G and hA3B mRNA in the peripheral blood mononuclear cells of Chinese HIV-infected individuals were found to be associated with slower HIV disease progression, suggesting their potential roles in antiviral innate immunity.
Zhao
Min
M
The First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Geng
Wenqing
W
Jiang
Yongjun
Y
Han
Xiaoxu
X
Cui
Hualu
H
Dai
Di
D
Bao
Mingjia
M
Pan
Ying
Y
Wang
Yating
Y
Zhang
Xiaoli
X
Zhang
Min
M
Qi
Guan
G
Shang
Hong
H
D019380:Anti-HIV Agents / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D018450:Disease Progression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy / Q000821:virology; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D019562:Viral Load
D019380:Anti-HIV Agents; D012333:RNA, Messenger