[Method of obtaining optical sectioning by grating spatial filter].
Based on the theory of digital spatial filter, we have developed a new method for obtaining optical sectioning by using a grating structured light in a conventional microscope. Theoretical derivation of technology was presented in the paper. In order to remove the unwanted grid pattern superimposed, we have changed the FFTs spectrum of image and have found a method better than the original linear method. Lastly we have explained how to realize the method as well as its advantage and disadvantage.
Li
Jing
J
Department of Medical Engineering, Kunming General Hospital, Kunming 650032, China. li_jingkm@126.com
Zhao
Haiyan
H
D002477:Cells / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008029:Lighting / Q000379:methods*; D008853:Microscopy / Q000379:methods*