title: Discrepancies between two automated immunoassay systems in determining hepatitis B virus markers in serum samples with concomitant presence of antigens and antibodies.
abstract: This study compared the assay results of HBV markers obtained with two automated immunoassay systems in serum samples with concomitant HBV antigens and antibodies. Eighty-nine samples of concomitant HBsAg and anti-HBs and 74 samples of concomitant HBeAg and anti-HBe were analyzed by the Abbott Architect i2000 and the Roche Elecsys 2010 immunoassay systems. The overall concordance rates of the Architect and Elecsys results were 78.6% for samples with concomitant HBsAg and anti-HBs and 77% for samples with concomitant HBeAg and anti-HBe. The data show that substantial differences exist between the results obtained by these two systems, especially in determining anti-HBs and HBeAg at low concentrations.
authors.affiliation: Department of Laboratory Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Cheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Guan
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Ziyong
authors.initials: Z
authors.lastname: Sun
mesh_terms: D001331:Automation; D015415:Biomarkers / Q000097:blood*; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006513:Hepatitis B e Antigens / Q000097:blood* / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D011379:Prognosis
chemical_list: D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens