title: Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution.
abstract: The gene of (all-E) geranylfarnesyl diphosphate synthase that is responsible for the biosynthesis of methanophenazine, an electron carrier utilized for methanogenesis, was cloned from a methanogenic archaeon Methanosarcina mazei Gö1. The properties of the recombinant enzyme and the results of phylogenetic analysis suggest that the enzyme is closely related to (all-E) prenyl diphosphate synthases that are responsible for the biosynthesis of respiratory quinones, rather than to the enzymes involved in the biosynthesis of archaeal membrane lipids, including (all-E) geranylfarnesyl diphosphate synthase from a thermophilic archaeon.
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ogawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hemmi
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D051231:Farnesyltranstransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D017020:Methanosarcina / Q000201:enzymology*; D008969:Molecular Sequence Data; D010619:Phenazines / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D010619:Phenazines; D011994:Recombinant Proteins; C111939:methanophenazine; D051231:Farnesyltranstransferase; D008697:Methane