Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells.
OBJECTIVE
Bacterial metabolites demineralize dental hard tissues, and soluble factors lead to tertiary dentinogenesis in the area of the dentin-pulp complex. However, it is unclear whether the oral bacteria are directly involved in the differentiation of dental pulp cells. In this study, we evaluated the effect of oral bacterial extracts on cellular differentiation in human dental pulp-derived cells (hDPC).


METHODS
The hDPC were obtained from third molar teeth, and the cells were subcultured. The sonicated extracts were obtained from Porphyromonas gingivalis (gram-negative) and Streptococcus mutans (gram-positive). The effect of bacterial extracts on cellular growth and differentiation in hDPC were tested.


RESULTS
Alkaline phosphatase activity and bone sialoprotein (BSP) gene expression were increased in hDPC exposed to low concentrations of both sonicated extracts, whereas the activity was decreased upon exposure to high concentrations of sonicated extracts from P. gingivalis.


CONCLUSIONS
This is the first evidence that oral bacteria have a positive effect on cellular differentiation in hPDC.
Abe
Shu
S
Division of Stem Sell Engineering, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Imaizumi
Mari
M
Mikami
Yoshikazu
Y
Wada
Yoshiyuki
Y
Tsuchiya
Shuhei
S
Irie
Seiko
S
Suzuki
Shinnosuke
S
Satomura
Kazuhito
K
Ishihara
Kazuyuki
K
Honda
Masaki J
MJ
D000704:Analysis of Variance; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003810:Dentinogenesis / Q000187:drug effects*; D004731:Endotoxins; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D010012:Osteogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D016966:Porphyromonas gingivalis; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D013010:Sonication; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans; D000099311:Dentin Sialophosphoprotein
D017077:Culture Media, Conditioned; D004731:Endotoxins; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C546871:IBSP protein, human