title: [Construction and immunogenicity evaluation of chimerical DNA vaccine of human papillomavirus type 11].
abstract: OBJECTIVE
  To construct chimerical DNA vaccine plasmid of human papillomavirus type 11 (HPV11) L1-E7, and to evaluate its immunogenicity.
  
  
  METHODS
  Molecular cloning techniques were used to construct recombinant plasmid pcDNA3 L1-E7 as a DNA vaccine. BALB/c mice were vaccinated with DNA recombinants through muscle injection.IL-2 and gamma-INF secreted by immunized spleens lymphocyte and HPV 11 L1 or E7 specific antibodies were assayed by ELISA method. Spleens lymphocyte proliferation was measured by MTT assay.
  
  
  RESULTS
  The chimerical DNA plasmid of pcDNA3 L1-E7 was constructed correctly. Specific anti-HPV11 E7 and L1 antibodies, specific lymphocyte proliferation and secretions of IL-2 and gamma-INF were detected in vaccinated mice.
  
  
  CONCLUSIONS
  Specific immune response, including cellular immunity and humoral immunity, could been detected in mice vaccinated with chimerical DNA vaccine of pcDNA3 L1-E7.
authors.affiliation: No.4 People's Hospital Affiliated to Suzhou University, Wuxi 214062, China.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Huang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Li
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Guo
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Liu
authors.forename: Jin-Dong
authors.initials: JD
authors.lastname: Ren
authors.forename: Ming-Xu
authors.initials: MX
authors.lastname: Song
authors.forename: Xi-Ke
authors.initials: XK
authors.lastname: Zhou
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Bi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D001483:Base Sequence; D005260:Female; D005818:Genetic Engineering; D052140:Human papillomavirus 11 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D011897:Random Allocation; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D053918:Papillomavirus Vaccines; D019444:Vaccines, DNA