title: Inhibition of histone deacetylase causes reduction of appressorium formation in the rice blast fungus Magnaporthe oryzae.
abstract: Post-translational modifications (PTMs) are important for cellular functions. The regulation of histone acetyltransferases (HATs) and histone deacetylases (HDACs) is one of important PTMs for epigenetic control, protein activity and protein stability. The regulation of acetylation of the N-terminal histone tails of core histone affects gene expression. Two class I HDAC genes and two class II HDAC genes have been identified in the Magnaporthe oryzae genome. Treatment with Rpd3/Hda1 family (classical) HDAC inhibitor inhibited the appressorium differentiation of M. oryzae. Treatment with trichostatin A, a classical HDAC inhibitor, also decreased pathogenesis. Furthermore, analyses of HDAC mutants indicated that MoHda1 and MoHos2 were required for vegetative growth and conidiation, and MoHos2 was required for appressorium formation. Disruption MoRPD3 was unsuccessful, as in the case with Aspergillus nidulans RpdA. These data indicated that HDACs have important roles in the asexual differentiation of M. oryzae.
authors.affiliation: Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Izawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Takekawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Arie
authors.forename: Tohru
authors.initials: T
authors.lastname: Teraoka
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamakura
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D020082:Magnaporthe / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity; D009154:Mutation; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D011499:Protein Processing, Post-Translational
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D006655:Histone Deacetylases