title: Glycyrrhetinic acid induces anoikis-like death and cytoskeletal disruption in the central nervous system tumorigenic cells.
abstract: We analyzed the effects of glycyrrhetinic acid (GA), a licorice compound, on the induction of anoikis-like death and cytoskeletal disruption in the central nervous system (CNS) tumorigenic cells. GA was cytotoxic in time- and dose-dependent manners, and the tumorigenic cells shed floating cells upon the GA treatment and even some of the adherent cells were easily detached from the fibronectin-coated culture dish by gentle shaking and aspiration. Reculture of the detached cells revealed that the longer the duration of GA exposure, the less the number of the proliferatable cells. These results indicate that GA perturbs cell adhesion and induces anoikis-like cell death. Further, GA also induced morphologic changes and disturbed cytoskeletal proteins.
authors.affiliation: Department of Pharmacy, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku, Nagoya 468-8503, Japan. hyamagu@ccmfs.meijo-u.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kidachi
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Kamiie
authors.forename: Toshiro
authors.initials: T
authors.lastname: Noshita
authors.forename: Hironori
authors.initials: H
authors.lastname: Umetsu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ryoyama
mesh_terms: D000818:Animals; D023102:Anoikis / Q000187:drug effects* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D016543:Central Nervous System Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003599:Cytoskeleton / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006034:Glycyrrhetinic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
chemical_list: D006034:Glycyrrhetinic Acid