Glycyrrhetinic acid induces anoikis-like death and cytoskeletal disruption in the central nervous system tumorigenic cells.
We analyzed the effects of glycyrrhetinic acid (GA), a licorice compound, on the induction of anoikis-like death and cytoskeletal disruption in the central nervous system (CNS) tumorigenic cells. GA was cytotoxic in time- and dose-dependent manners, and the tumorigenic cells shed floating cells upon the GA treatment and even some of the adherent cells were easily detached from the fibronectin-coated culture dish by gentle shaking and aspiration. Reculture of the detached cells revealed that the longer the duration of GA exposure, the less the number of the proliferatable cells. These results indicate that GA perturbs cell adhesion and induces anoikis-like cell death. Further, GA also induced morphologic changes and disturbed cytoskeletal proteins.
Yamaguchi
Hideaki
H
Department of Pharmacy, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku, Nagoya 468-8503, Japan. hyamagu@ccmfs.meijo-u.ac.jp
Kidachi
Yumi
Y
Kamiie
Katsuyoshi
K
Noshita
Toshiro
T
Umetsu
Hironori
H
Ryoyama
Kazuo
K
D000818:Animals; D023102:Anoikis / Q000187:drug effects* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D016543:Central Nervous System Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003599:Cytoskeleton / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006034:Glycyrrhetinic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
D006034:Glycyrrhetinic Acid