Liquid chromatographic analysis of oxcarbazepine and its metabolites in plasma and saliva after a novel microextraction by packed sorbent procedure.
A rapid and reliable analytical method suitable for the simultaneous determination of the antiepileptic drug, oxcarbazepine and its metabolites in human plasma and saliva by means of liquid chromatography with diode array detection (DAD) has been developed. Oxcarbazepine and its metabolites (10,11-dihydro-10-hydroxycarbamazepine, trans-10,11-dihydro-10,11-dihydroxycarbamazepine and 3-hydroxycarbamazepine) were baseline separated within 6.5 min on a reversed-phase C18 column with a phosphate buffer-acetonitrile-triethylamine mixture as the mobile phase. The DAD detector was set at 240 nm. A sample preparation method for biological samples using a microextraction by packed sorbent technique has been implemented, employing a C18 sorbent inserted into a microvolume syringe and using only a small volume (25 microL) of plasma or saliva. The extraction yield values were satisfactory for all analytes (>86.5%) as well as the precision data, which were always in the low percentage of relative standard deviation values (<4.6%). The method was successfully applied to both plasma and saliva samples drawn from psychiatric and neurological patients undergoing treatment with oxcarbazepine (Tolep) tablets.
Saracino
Maria Addolorata
MA
Department of Pharmaceutical Sciences, Laboratory of Pharmaco-Toxicological Analysis, Faculty of Pharmacy, University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.
Tallarico
Katianna
K
Raggi
Maria Augusta
MA
D000327:Adsorption; D053000:Analytic Sample Preparation Methods; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D002853:Chromatography, Liquid; D019963:Clinical Chemistry Tests / Q000379:methods*; D006801:Humans; D000078330:Oxcarbazepine; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism*; D052617:Solid Phase Microextraction / Q000379:methods*
D002220:Carbamazepine; D000078330:Oxcarbazepine