title: BODIPY probes to study peroxisome dynamics in vivo.
abstract: There is a continuing need for bioprobes that are target-specific and combine speed of delivery with maintenance of normal cell behaviour. Towards this end, we are developing small pro-fluorescent molecules that provide such specificity through chemical activation by biomolecules. We have generated a set of BODIPY (boron dipyrromethane) fluorophores, including one that is intrinsically non-fluorescent but on incubation with cells becomes fluorescent at its target site. Addition of these BODIPY probes to plant cells identifies peroxisomes, as verified by co-localization with an SKL-FP construct. Interestingly, in mammalian cells, co-localization with the mammalian peroxisomal marker SelectFX(TM) was not observed. These data suggest fundamental differences in peroxisome composition, development or function between plant and animal cells.
authors.affiliation: Centre for Bioactive Chemistry, Department of Chemistry, University of Durham, Science Laboratories, South Road, Durham, DH1 3LE, UK.
authors.forename: Marie
authors.initials: M
authors.lastname: Landrum
authors.forename: Andrei
authors.initials: A
authors.lastname: Smertenko
authors.forename: Robert
authors.initials: R
authors.lastname: Edwards
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hussey
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Steel
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D020675:Peroxisomes / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes