Rapid identification and preparative isolation of antioxidant components in licorice.
This study employed the online HPLC-2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate radical cation (ABTS(+*)) bioassay to rapidly determine antioxidant compounds occurring in the licorice extract of Glycyrrhiza uralensis. The negative peaks of the ABTS(+*) radical scavenging detection system, which indicated the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The ABTS(+)-based antioxidant activity profile showed that three peaks exhibited antioxidant activity, and then the high-speed counter-current chromatography technique of preparative scale was successfully applied to separate the three peaks I-III in one step from the licorice extract. The high-speed counter-current chromatography was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (6.5:5.5:6:4, v/v). Yields of the three peaks, dehydroglyasperin C (I, 95.1% purity), dehydroglyasperin D (II, 96.2% purity), and isoangustone A (III, 99.5% purity), obtained were 10.33, 10.43, and 6.7% respectively. Chemical structures of the purified dehydroglyasperin C (I), dehydroglyasperin D (II), and isoangustone A (III) were identified by ESI-MS and (1)H- and (13)C-NMR analysis.
Lee
Yeon Sil
YS
Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Republic of Korea.
Kim
Seon Ha
SH
Kim
Jin Kyu
JK
Shin
Hyun-Kyung
HK
Kang
Young-Hee
YH
Park
Jung Han Yoon
JH
Lim
Soon Sung
SS
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D001578:Benzopyrans / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006035:Glycyrrhiza / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D013997:Time Factors
D000975:Antioxidants; D001578:Benzopyrans; D005419:Flavonoids; D007529:Isoflavones; C551631:isoangustone A; C548688:dehydroglyasperin C; C551630:dehydroglyasperin D