B-cell-T-cell activation and interaction in common variable immunodeficiency.
Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help.
Rezaei
Nima
N
Department of Infection and Immunity, The University of Sheffield, Sheffield, UK. nima_rezaei@farabi.tums.ac.ir
Wing
James B
JB
Aghamohammadi
Asghar
A
Carlring
Jennifer
J
Lees
Andrew
A
Asgarian-Omran
Hossein
H
Pourpak
Zahra
Z
Sarrafnejad
Abdolfattah
A
Kardar
Gholam A
GA
Shahrestani
Tahereh
T
Masoumi
Farimah
F
Zare
Ahad
A
Saghafi
Shiva
S
Sarrafzadeh
Shokouh
S
Foster
Rachel A
RA
Heath
Andrew W
AW
Read
Robert C
RC
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002478:Cells, Cultured; D017074:Common Variable Immunodeficiency / Q000097:blood / Q000276:immunology* / Q000473:pathology; D003911:Dextrans / Q000378:metabolism; D005260:Female; D006801:Humans; D007114:Immunization; D007072:Immunoglobulin D / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D019899:Paracrine Communication; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D003911:Dextrans; D007072:Immunoglobulin D