title: The effect of cultured autologous periodontal ligament cells on the healing of delayed autotransplanted dog's teeth.
abstract: BACKGROUND
  The regeneration of the periodontal structure for avulsed teeth extended dry times has been a goal of dentists. The aim of this study was to investigate a new strategy of delayed replantation for avulsed teeth that were not suitable for immediate replantation.
  
  
  METHODS
  Extracted dog's premolar teeth were maintained in a dry environment for a month after isolation and proliferation of the periodontal ligament (PDL) cells. Then, tooth roots coated with 1 x 10(6) cultured autologous PDL cells were autotransplanted in artificial sockets created in the mandible. The dogs were sacrificed 60 days after transplantation. Histologic analyses showed that a root-PDL-bone complex was found in all cases of the PDL cell-loaded samples.
  
  
  RESULTS
  The new PDL-like connective tissue was located between the alveolar bone and the transplanted roots, with fibers inserting into the newborn cementum on one end and alveolar bone on the other. For the control samples, no PDL-like tissue was found, and ankylosis was commonly observed.
  
  
  CONCLUSIONS
  The results indicated that cultured autologous PDL cells assist in the re-establishment of periodontal architecture of autotransplanted teeth that is devoid of viable periodontal cells.
authors.affiliation: Research Laboratory of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Gary Shun-pan
authors.initials: GS
authors.lastname: Cheung
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Xu
authors.forename: Shijie
authors.initials: S
authors.lastname: Zhao
authors.forename: Chengfei
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001641:Bicuspid / Q000637:transplantation; D018929:Cell Culture Techniques; D017690:Cell Transplantation; D004285:Dogs; D006085:Graft Survival; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010513:Periodontal Ligament / Q000166:cytology* / Q000637:transplantation; D010519:Periodontium / Q000166:cytology / Q000502:physiology; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014090:Tooth Replantation / Q000379:methods*; D019553:Tooth, Nonvital; D014182:Transplantation, Autologous
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate