title: Transgenic inhibition of glial NF-kappa B reduces pain behavior and inflammation after peripheral nerve injury.
abstract: The transcription factor nuclear factor kappa B (NF-kappaB) is a key regulator of inflammatory processes in reactive glial cells. We utilized a transgenic mouse model (GFAP-IkappaBalpha-dn) where the classical NF-kappaB pathway is inactivated by overexpression of a dominant negative (dn) form of the inhibitor of kappa B (IkappaBalpha) in glial fibrillary acidic protein (GFAP)-expressing cells, which include astrocytes, Schwann cells, and satellite cells of the dorsal root ganglion (DRG) and sought to determine whether glial NF-kappaB inhibition leads to a reduction in pain behavior and inflammation following chronic constriction injury (CCI) of the sciatic nerve. As expected, following CCI nuclear translocation, and hence activation, of NF-kappaB was detected only in the sciatic nerve of wild type (WT) mice, and not in GFAP-IkappaBalpha-dn mice, while upregulation of GFAP was observed in the sciatic nerve and DRGs of both WT and GFAP-IkappaBalpha-dn mice, indicative of glial activation. Following CCI, mechanical and thermal hyperalgesia were reduced in GFAP-IkappaBalpha-dn mice compared to those in WT, as well as gene and protein expression of CCL2, CCR2 and CXCL10 in the sciatic nerve. Additionally, gene expression of TNF, CCL2, and CCR2 was reduced in the DRGs of transgenic mice compared to those of WT after CCI. We can therefore conclude that transgenic inhibition of NF-kappaB in GFAP-expressing glial cells attenuated pain and inflammation after peripheral nerve injury. These findings suggest that targeting the inflammatory response in Schwann cells and satellite cells may be important in treating neuropathic pain.
authors.affiliation: Department of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL 33136, United States The Neuroscience Program, University of Miami Miller School of Medicine, Miami, FL 33136, United States The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
authors.forename: Eugene S
authors.initials: ES
authors.lastname: Fu
authors.forename: Yan Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Sagen
authors.forename: Keith A
authors.initials: KA
authors.lastname: Candiotti
authors.forename: Paul D
authors.initials: PD
authors.lastname: Morton
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Liebl
authors.forename: John R
authors.initials: JR
authors.lastname: Bethea
authors.forename: Roberta
authors.initials: R
authors.lastname: Brambilla
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D007839:Functional Laterality / Q000502:physiology; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein; D006930:Hyperalgesia / Q000503:physiopathology; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000172:deficiency* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000378:metabolism; D017288:Pain Threshold / Q000502:physiology*; D012584:Sciatic Nerve / Q000378:metabolism / Q000473:pathology; D020426:Sciatic Neuropathy / Q000150:complications*; D013997:Time Factors
chemical_list: D016207:Cytokines; D005904:Glial Fibrillary Acidic Protein; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; C528900:glial fibrillary astrocytic protein, mouse