Specific induced circular dichroism and enhanced B to z transitions of duplexes stabilized by chromophore-linked alkynylnucleoside residues.
We have developed an induced circular dichroism (ICD) probe with a chromophore-linked alkynyldeoxyribose skeleton for analyzing higher-order structures of DNA duplexes in the visible-light region. When CG-repeated oligonucleotides (ODNs) with the probe at their 5' ends adopted Z-form duplexes at a high NaCl concentration, strong ICD signals were observed at the absorptive region of the chromophore. On the other hand, their B-form duplexes, formed at a low NaCl concentration, produced a faint ICD signal. The specific ICD for the Z-form duplexes was found to appear only when the chromophores were attached at the 5' ends of each of the ODNs. Furthermore, the chromophoric alkynylnucleoside residues effectively promoted the B to Z transition of the ODN.
Fujimoto
Kazuhisa
K
Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan. fujimoto@pha.u-toyama.ac.jp
Aizawa
Sayaka
S
Oota
Ikko
I
Chiba
Junya
J
Inouye
Masahiko
M
D000480:Alkynes / Q000737:chemistry*; D020029:Base Pairing; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry*; D010777:Photochemistry; D013237:Stereoisomerism
D000480:Alkynes; D009705:Nucleosides; D004247:DNA