Hairpin DNA probe based surface plasmon resonance biosensor used for the activity assay of E. coli DNA ligase.
Using hairpin DNA probe self-structure change during DNA ligation process, a sensitive, label-free and simple method of E. coli DNA ligase assay via a home-built high-resolution surface plasmon resonance (SPR) instrument was developed. The DNA ligation process was monitored in real-time and the effects of single-base mutation on the DNA ligation process were investigated. Then an assay of E. coli DNA ligase was completed with a lower detection limit (0.6 nM), wider concentration range and better reproducibility. Moreover, the influence of Quinacrine on the activity of E. coli DNA ligase was also studied, which demonstrated that our method was useful for drug screening.
Luan
Qingfen
Q
College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing, 100049, China.
Xue
Ying
Y
Yao
Xin
X
Lu
Wu
W
D001681:Biological Assay; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000378:metabolism*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D020349:Surface Plasmon Resonance
D015342:DNA Probes; D004269:DNA, Bacterial; D011088:DNA Ligases; D000072481:DNA Ligase ATP