Structure of bovine pancreatic ribonuclease complexed with uridine 5'-monophosphate at 1.60 A resolution.
Bovine pancreatic ribonuclease A (RNase A) was crystallized from a mixture of small molecules containing basic fuchsin, tobramycin and uridine 5'-monophosphate (U5P). Solution of the crystal structure revealed that the enzyme was selectively bound to U5P, with the pyrimidine ring of U5P residing in the pyrimidine-binding site at Thr45. The structure was refined to an R factor of 0.197 and an R(free) of 0.253.
Larson
Steven B
SB
Department of Molecular Biology and Biochemistry, The University of California, Irvine, CA 92697-3900, USA.
Day
John S
JS
Nguyen
Chieugiang
C
Cudney
Robert
R
McPherson
Alexander
A
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D018360:Crystallography, X-Ray; D008024:Ligands; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014542:Uridine Monophosphate / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D014542:Uridine Monophosphate; D012259:Ribonuclease, Pancreatic