2D DIGE of the cerebrospinal fluid proteome in neurological diseases.
2D DIGE is a promising approach to comparative proteome analysis known for a high sensitivity and high reproducibility compared with classical 2DE techniques. It offers new possibilities for the detection of cerebrospinal fluid (CSF) biomarkers in neurological diseases, such as dementia, amyotrophic lateral sclerosis or multiple sclerosis. We review the first studies using 2D DIGE for analysis of the CSF proteome in neurological diseases and discuss advantages, as well as drawbacks, of the methodological approach with special emphasis on CSF-related aspects.
Tumani
Hayrettin
H
Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany. hayrettin.tumani@uni-ulm.de
Lehmensiek
Vera
V
Lehnert
Stefan
S
Otto
Markus
M
Brettschneider
Johannes
J
D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods
D002556:Cerebrospinal Fluid Proteins; D020543:Proteome