Complete genome sequence of the multiresistant taxonomic outlier Pseudomonas aeruginosa PA7.
Pseudomonas aeruginosa PA7 is a non-respiratory human isolate from Argentina that is multiresistant to antibiotics. We first sequenced gyrA, gyrB, parC, parE, ampC, ampR, and several housekeeping genes and found that PA7 is a taxonomic outlier. We report here the complete sequence of the 6,588,339 bp genome, which has only about 95% overall identity to other strains. PA7 has multiple novel genomic islands and a total of 51 occupied regions of genomic plasticity. These islands include antibiotic resistance genes, parts of transposons, prophages, and a pKLC102-related island. Several PA7 genes not present in PAO1 or PA14 are putative orthologues of other Pseudomonas spp. and Ralstonia spp. genes. PA7 appears to be closely related to the known taxonomic outlier DSM1128 (ATCC9027). PA7 lacks several virulence factors, notably the entire TTSS region corresponding to PA1690-PA1725 of PAO1. It has neither exoS nor exoU and lacks toxA, exoT, and exoY. PA7 is serotype O12 and pyoverdin type II. Preliminary proteomic studies indicate numerous differences with PAO1, some of which are probably a consequence of a frameshift mutation in the mvfR quorum sensing regulatory gene.
Roy
Paul H
PH
Département de biochimie et de microbiologie, and Centre de recherche en infectiologie, Université Laval, Québec, Québec, Canada.
Tetu
Sasha G
SG
Larouche
André
A
Elbourne
Liam
L
Tremblay
Simon
S
Ren
Qinghu
Q
Dodson
Robert
R
Harkins
Derek
D
Shay
Ryan
R
Watkins
Kisha
K
Mahamoud
Yasmin
Y
Paulsen
Ian T
IT
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D016680:Genome, Bacterial; D008826:Microbial Sensitivity Tests; D020543:Proteome; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D053038:Quorum Sensing; D037521:Virulence Factors / Q000235:genetics
D000900:Anti-Bacterial Agents; D020543:Proteome; D037521:Virulence Factors