[Diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-Vetiver zizanioides].
OBJECTIVE
Vetiver zizanioides is a perennial grass of the Poaceae family, known of its silage, soil and water conservation role. The aim of the study was to collect and identify the resources of the nitrogen-fixing bacteria associated with Vetiver zizanioides.


METHODS
Associated nitrogen-fixing bacteria isolated from Vetiver zizanioides were studied by SDS-PAGE whole-cell protein patterns, insert sequence (IS)-PCR finger printing, utilization of sole carbon sources and 16S rRNA gene sequence analysis.


RESULTS
Based on the results of finger printing analysis, protein patterns and biological test, isolates were grouped into 6 clusters, except 4 single strains. Phylogenetic analysis of 16S rDNA sequences indicated that isolates belonged to Herbaspirillum frisingense, Enterobacter ludwigii, Pseudacidovorax intermedius, Mitsuaria chitosanitabida, Pseudomonas putida, Pseudomonas fluorescens, Burkholderia vietnamiensis and Enterobacter cloacae.


CONCLUSIONS
The nitrogen fixers associated with Vetiver zizanioides showed great diversity and may have a potential application for the grass forage and agriculture.
Zhao
Xianwei
X
Guangdong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. 2006215016@stu.scau.edu.cn
Javed
Chaudhary Hassan
CH
He
Yumei
Y
Zhang
Zhiying
Z
Peng
Guixiang
G
Tan
Zhiyuan
Z
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D044822:Biodiversity; D002681:China; D049930:Chrysopogon / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S