[The frequency and function of FoxP3+ regulatory T cell in patients with acute hepatitis B].
OBJECTIVE
To investigate the dynamic variety of frequency and function of FoxP3+ regulatory T cells in patients with acute hepatitis B (AHB).


METHODS
Peripheral blood mononuclear cells (PBMCs) from 15 AHB patients at acute phase (week 1 of illness), convalescent phase (primary occurrence of both ALT level normalization and HBsAg negative conversion), resolved phase (at least 8 weeks after both ALT normalization and HBsAg seroconversion, and 15 health subjects were analyzed for FoxP3 (Forkhead/winged helix transcription factor) mRNA expression in MACS magnetic beads-purified CD4+ T cells by real-time RT-PCR assay. The effects of Treg cells on the proliferation of CD4+ CD25- T cells were examined by a 3H-thymidine incorporation assay.


RESULTS
AHB patients presented a significantly higher FoxP3 mRNA expression at convalescent phase than acute phase (t= -6.04, P<0.01) and resolved phase (t=4.45, P<0.01), and healthy controls (t=3.44, P<0.01). We also observed that the suppression efficiency of Treg cells on proliferation of CD4+ CD25- T cells was lower at acute phase than convalescent phase (t= -5.30, P<0.01) and resolved phase (t= -3.20, P<0.05), but there was no significant difference between healthy controls and any phase of AHB.


CONCLUSIONS
AHB patients presented lower circulating Treg frequency and suppression function at acute phase, and both of them are increase at convalescent phase, and then return to normal level along with disease resolved. This follow-up study furthers our understanding of Treg's role in immunopathogenesis of hepatitis B.
Qin
Chen-xi
CX
Department of Clinical Laboratory, 263 Hospital Affiliateda to General Hospital of Beijing Military Region of PLA, Beijing, China. qinchenxi003@163.com
Gao
Hong-wei
HW
Zhang
Ying
Y
Gao
Lei
L
Jia
Feng
F
Zou
Yong-hong
YH
Yang
Xiu-qing
XQ
Cuo
Xue-qing
XQ
D000328:Adult; D016022:Case-Control Studies; D002452:Cell Count; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006509:Hepatitis B / Q000097:blood / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger