title: Gastritis induced by Helicobacter pylori infection in experimental rats.
abstract: BACKGROUND
  Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide.
  
  
  OBJECTIVE
  Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination.
  
  
  METHODS
  Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-year-old male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E(2) (PGE(2)) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis.
  
  
  RESULTS
  Serum gastrin, IL-6, mucosal MPO activity, and PGE(2) demonstrated significant increases joined with a decreased serum pepsinogen I activity (P < 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation.
  
  
  CONCLUSIONS
  Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt. mmelseweidy@yahoo.com
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Elseweidy
authors.forename: Mona M
authors.initials: MM
authors.lastname: Taha
authors.forename: Nahla N
authors.initials: NN
authors.lastname: Younis
authors.forename: Khadiga S
authors.initials: KS
authors.lastname: Ibrahim
authors.forename: Hamdi A
authors.initials: HA
authors.lastname: Hamouda
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Eldosouky
authors.forename: Hala
authors.initials: H
authors.lastname: Soliman
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D053938:DNA Fragmentation; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005755:Gastrins / Q000378:metabolism; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism* / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D020116:Pepsinogen A / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011706:Pyloric Antrum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D005755:Gastrins; D015850:Interleukin-6; D020116:Pepsinogen A; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D015232:Dinoprostone