Crypt abscess-associated microbiota in inflammatory bowel disease and acute self-limited colitis.
OBJECTIVE
To evaluate whether crypt abscesses from inflammatory bowel disease (IBD) patients contain bacteria and to establish their nature.


METHODS
We studied 17 ulcerative colitis patients, 11 Crohn's disease patients, 7 patients with acute self-limited colitis (ASLC) and normal colonic biopsies from 5 subjects who underwent colonoscopy for colon cancer screening. A fluorescent in situ hybridization technique was applied to colonic biopsies to assess the microbiota composition of the crypts and crypt abscesses.


RESULTS
Crypts colonized by bacteria were observed in 42.9% and 3.6% of ASLC and IBD patients, respectively (P = 0.019). Crypt abscesses colonized by bacteria were observed in 28.6% and 0.0% of ASLC and IBD patients, respectively (P = 0.035).


CONCLUSIONS
These results do not support the hypothesis that crypt abscesses in IBD are the result of localized dysbiosis arising from persistence of living bacteria colonizing the crypts.
Sokol
Harry
H
Gastroenterology and Nutrition Department, Saint Antoine Hospital, Pierre et Marie Curie-Paris6 University, AP-HP, 184 rue du Faubourg Saint-Antoine, 75571, Paris, France. sokol_harry@yahoo.fr
Vasquez
Nadia
N
Hoyeau-Idrissi
Nadia
N
Seksik
Philippe
P
Beaugerie
Laurent
L
Lavergne-Slove
Anne
A
Pochart
Philippe
P
Marteau
Philippe
P
D000038:Abscess / Q000382:microbiology* / Q000473:pathology; D000328:Adult; D003092:Colitis / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000382:microbiology / Q000473:pathology; D003113:Colonoscopy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015212:Inflammatory Bowel Diseases / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged