Nano-encapsulation of lipase by self-assembled nanogels: induction of high enzyme activity and thermal stabilization.
The effects of self-assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially k(cat), drastically increased in the presence of nanogels of cholesterol-bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 degrees C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization-induced aggregation. Nano-encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme.
Sawada
Shin-ichi
S
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Akiyoshi
Kazunari
K
D055162:Biocatalysis; D016956:Burkholderia cepacia / Q000201:enzymology; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry; D002850:Chromatography, Gel; D002942:Circular Dichroism; D004337:Drug Carriers / Q000737:chemistry*; D004795:Enzyme Stability; D005612:Freeze Drying; D005936:Glucans / Q000737:chemistry; D006358:Hot Temperature; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000008:administration & dosage / Q000378:metabolism*; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D011487:Protein Conformation; D011489:Protein Denaturation
D004337:Drug Carriers; D005936:Glucans; D020100:Hydrogels; C009109:pullulan; D002784:Cholesterol; D008049:Lipase