Overexpression of SATB1 in laryngeal squamous cell carcinoma.
OBJECTIVE
To investigate the expression of special AT-rich sequence-binding protein 1 (SATB1), and the relationship between SATB1 and clinicopathological factors of laryngeal squamous cell carcinoma (LSCC).


METHODS
Real-time PCR, Western blot and immunohistochemistry were used to analyze 80 samples of LSCC and 25 samples of control mucosa. The relationship between SATB1 expression and clinicopathological parameters was evaluated.


RESULTS
The SATB1 mRNA expression levels in LSCC were 2.5- to 7.5-fold higher than those in control mucosa tissues (p < 0.001). SATB1 protein expression was detected in approximately 66% (53 out of 80) of the LSCC specimens, whereas it was below the detection limit in all the control mucosa specimens (p < 0.001). The SATB1 mRNA levels in positive cervical lymph nodes, in clinical stages III and IV with poor/moderate cell differentiation, were significantly higher than those in negative cervical lymph nodes in clinical stage II with high cell differentiation (p = 0.022, p = 0.005 and p = 0.006, respectively).


CONCLUSIONS
SATB1 mRNA and protein levels are elevated in LSCC tissues, and their levels are correlated with clinical stages and differentiation status. The current findings suggest that SATB1 may be a useful marker for the prognosis and assessment of therapeutic effects.
Zhao
Xu-Dong
XD
Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Shenyang, PR China.
Ji
Wen-yue
WY
Zhang
Wei
W
He
Li-Xia
LX
Yang
Jing
J
Liang
Hong-Jun
HJ
Wang
Li-Li
LL
D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D016009:Chi-Square Distribution; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008207:Lymphatic Metastasis; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human