title: Minimally permutated peptide analogs as internal standards for relative and absolute quantification of peptides and proteins.
abstract: A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differentiated from their target analytes by LC-MS due to individual retention times and/or by MS/MS due to specific fragment ions. Both quantification methods are demonstrated using peptide mixtures of low and high complexity.
authors.affiliation: Molecular Structure Analysis, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Dominic
authors.initials: D
authors.lastname: Winter
authors.forename: Joerg
authors.initials: J
authors.lastname: Seidler
authors.forename: Dominik
authors.initials: D
authors.lastname: Kugelstadt
authors.forename: Bianca
authors.initials: B
authors.lastname: Derrer
authors.forename: Barbara
authors.initials: B
authors.lastname: Kappes
authors.forename: Wolf D
authors.initials: WD
authors.lastname: Lehmann
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D040901:Proteomics / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; C509918:glideosome-associated protein 45, Plasmodium falciparum