Hexaminolevulinate-mediated photodynamic purging of leukemia cells from BM.
Photodynamic therapy (PDT) with porphyrin precursors has been established for tumor treatment. This study aimed at examining applicability of hexaminolevulinate (HAL) for photodynamic purging of leukemic cells from BM grafts and evaluating the clinical relevance of in vitro models. The PDT dose resulting in no colony formation by leukemic cells in vitro, in pure form or in a mixture with BM cells, was insufficient for complete killing of the leukemic cells ex vivo and for the treatment of the leukemia-bearing animals in vivo. The efficacy of HAL-PDT in cell lines in vitro should be verified in clinically relevant in vivo models.
Čunderlíková
B
B
Department of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
Vasovič
V
V
Sieber
F
F
Furre
T
T
Nesland
J M
JM
Peng
Q
Q
D000622:Aminolevulinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry / Q000187:drug effects*; D016465:Bone Marrow Purging / Q000379:methods*; D016026:Bone Marrow Transplantation; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005720:Gamma Rays; D006098:Granulocytes / Q000737:chemistry / Q000187:drug effects; D007939:Leukemia L1210 / Q000401:mortality / Q000628:therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D009994:Osmolar Concentration; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000032:analysis; D016019:Survival Analysis; D014410:Tumor Stem Cell Assay; D014916:Whole-Body Irradiation / Q000401:mortality
D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; C419924:5-aminolevulinic acid hexyl ester