title: [Epitope screening of influenza A (H3N2) by using phage display library].
abstract: OBJECTIVE
  To screen the influenza A (H3N2) mimotopes by using phage display library.
  
  
  METHODS
  Using influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
  
  
  RESULTS
  21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2).
  
  
  CONCLUSIONS
  Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.
authors.affiliation: Viral hepatitis research laboratary, Institute of Infectious Disease of P1A 302 Hospital, Beijing 100039, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Zhong
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.forename: Jiw-Zeng
authors.initials: JZ
authors.lastname: Dai
authors.forename: Biao
authors.initials: B
authors.lastname: Xu
authors.forename: Le
authors.initials: L
authors.lastname: Li
mesh_terms: D018604:Epitope Mapping; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library
chemical_list: D019151:Peptide Library