title: Proteomic analysis of time-dependent difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells.
abstract: The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal development in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be activated in cultured embryonic stem cells and our two dimensional electrophoresis data should be useful for investigating some of the mechanism that regulates neuronal differentiation.
authors.affiliation: Department of Biological science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Shim
authors.forename: Sun A
authors.initials: SA
authors.lastname: Cho
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Na Kyung
authors.initials: NK
authors.lastname: Ryu
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Yang
authors.forename: Seyoon
authors.initials: S
authors.lastname: Kim
authors.forename: Young Yil
authors.initials: YY
authors.lastname: Bahk
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006863:Hydrogen-Ion Concentration; D051379:Mice; D055495:Neurogenesis / Q000187:drug effects / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D014212:Tretinoin