Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (microFIC).
Microfluidic systems have significant implications in the field of in vitro cell-based assays since they may allow conventional cell-based assays to be conducted in an automated and high-throughput fashion. In this study, we combined a simple microfluidic cells-on-chip system with a morphology-based image cytometric analysis approach for the assessment of Cd(2+) induced apoptosis of Chang liver cell line. A simple and efficient in situ monitoring method for quantifying the progress of a cell death event was developed and is presented here. Reasonable agreement of the estimated EC(50) value from this study with those from the literature and a close correlation between the observed changes in cell morphology (i.e., circularity) and the amount of reactive oxygen species (ROS) generation confirmed the validity of this morphology-based microfluidic image cytometric (microFIC) assessment method. We propose this morphology-based microFIC approach as an easy and efficient way to assess cytotoxicity which can be adapted to high-throughput screening platforms for in vitro cytotoxicity assays as well as drug screening.
Kim
Min Jung
MJ
Dept. of Chemistry, Hanyang University, Seoul, 133-791, Korea.
Lim
Kook Hee
KH
Yoo
Hyun Ju
HJ
Rhee
Seog Woo
SW
Yoon
Tae Hyun
TH
D017209:Apoptosis / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D002151:Calorimetry; D002452:Cell Count; D002460:Cell Line; D057166:High-Throughput Screening Assays; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
D002104:Cadmium