Global gene expression analysis of lenses from different mouse strains and in the alpha3Cx46 knockout mouse.
OBJECTIVE
Disruption of the mouse gene encoding the gap junction subunit alpha3 connexin 46 (alpha3Cx46) results in the formation of lens cataracts that have a severity affected by the genetic background of the mouse strain. To identify the genes that influence the severity of the nuclear opacity, global gene expression was analyzed in lenses from the 129SvJae strain and compared to the C57BL/6J strain.


METHODS
Lens transcripts were subjected to cDNA microarray analysis. Results on selected genes were confirmed by real-time PCR.


RESULTS
GENES THAT WERE DETERMINED TO BE ALTERED IN EXPRESSION LEVELS AS A RESULT OF STRAIN DIFFERENCES COULD BE CLUSTERED INTO THREE GROUPS: energy metabolism, stress response, and cell growth.


CONCLUSIONS
There were no observed changes in gene expression as a result of the lack of alpha3Cx46 in the different mouse strains, suggesting that the pathways mediated by this connexin do not influence gene transcription in the lens. Analysis of the transcript changes due to strain differences provides new insights into potential genetic modifiers of cataractogenesis. More detailed experimentation will be needed to determine if these observed changes do indeed affect cataractogenesis.
Tang
Yajun
Y
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
Crowley
Thomas E
TE
Kumar
Nalin M
NM
D000818:Animals; D017630:Connexins / Q000172:deficiency* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018390:Gene Targeting; D007908:Lens, Crystalline / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D021122:Protein Subunits / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013045:Species Specificity; D014158:Transcription, Genetic
D017630:Connexins; D021122:Protein Subunits; D012333:RNA, Messenger; C082917:GJA3 protein, human