Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats.
OBJECTIVE
To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats.


METHODS
Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number.


RESULTS
After 16 weeks, mean of apoptotic cells number in control group (19,3 + or - 3,2) was greater than 40mg/Kg group (11,8 + or - 1,9; p<0,01) and 80mg/Kg group (7,0 + or - 1,4; p<0,01). Significant difference also observed between 40mg/Kg and 80mg/Kg (p<0,05). After 18 weeks, mean of apoptotic cells number in control group (18,0 + or - 2,2) was greater than 40mg/Kg group (8,8 + or - 1,7; p<0,01) and 80mg/Kg group (5,5 + or - 1,3; p<0,01). Significant difference wasn't observed between 40mg/Kg and 80mg/Kg (ns).


CONCLUSIONS
Intra-pulmonary instillation of Benzo[a]pyrene induces significant decrease of apoptotic activity in lung tissue.
Silva
Baldomero Antonio Kato da
BA
Central West Region, UFMS, MS, Brazil. ftbaldock@uol.com.br
Aydos
Ricardo Dutra
RD
Silva
Iandara Schettert
IS
Pereira
Daniel Martins
DM
Carvalho
Paulo de Tarso Camillo de
Pde T
Dourado
Doroty Mesquita
DM
Reis
Filipe Abdalla dos
FA
Nacer
Renato Silva
RS
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000008:administration & dosage*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D001564:Benzo(a)pyrene