title: Clinical isolates of Enterococcus faecalis aggregate human platelets.
abstract: Many endocarditis pathogens activate human platelets and this has been proposed to contribute to virulence. Here we report for the first time that many clinical isolates of Enterococcus faecalis, a common pathogen in infective endocarditis, aggregate human platelets. 84 isolates from human blood and urine were screened for their ability to aggregate platelets from four different donors. Platelet aggregation occurred for between 11 and 65% of isolates depending on the donor. In one donor, a significantly larger proportion of isolates from blood than from urine caused platelet aggregation. Median time to aggregation was 11 min and had a tendency to be shorter for blood isolates as compared to urine isolates. Immunoglobulin G (IgG) was shown to be essential in mediating activation and aggregation. Platelet aggregation could be abolished by an IgG-specific proteinase (IdeS), by an antibody blocking FcRgammaIIa on platelets, or by preabsorption of plasma with an E. faecalis isolate. Fibrinogen binding to bacteria or platelets does not contribute to platelet activation or aggregation under our experimental conditions. These results indicate that platelet activation and aggregation by E. faecalis is dependent on both host and bacterial factors and that it may be involved in the pathogenesis of invasive disease with this organism.
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Lund, Sweden. magnus.rasmussen@med.lu.se
authors.forename: Magnus
authors.initials: M
authors.lastname: Rasmussen
authors.forename: Daniel
authors.initials: D
authors.lastname: Johansson
authors.forename: Sara K
authors.initials: SK
authors.lastname: Söbirk
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Oonagh
authors.initials: O
authors.lastname: Shannon
mesh_terms: D001769:Blood / Q000382:microbiology; D001792:Blood Platelets / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000302:isolation & purification / Q000472:pathogenicity*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D015539:Platelet Activation; D010974:Platelet Aggregation; D013997:Time Factors; D014556:Urine / Q000382:microbiology
chemical_list: D007074:Immunoglobulin G