title: Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL.
abstract: Low-density lipoprotein (LDL) particles are the major cholesterol-carrying lipoprotein in the human circulation from the liver to peripheral tissues. High levels of LDL-Cholesterol (LDL-C) are known risk factor for the development of coronary artery disease (CAD). The most common approach to determine the LDL-C in the clinical laboratory involves the Friedewald formula. However, in certain situations, this approach is inadequate. In this paper we report on the enhancement on the Europium emission band of Europium chlortetracycline complex (CTEu) in the presence of LDL. The emission intensity at 615 nm of the CTEu increases with increasing amounts of LDL. This phenomenon allowed us to propose a method to determine the LDL concentration in a sample composed by an aqueous solution of LDL. With this result we obtained LDL calibration curve, LOD (limit of detection) of 0.49 mg/mL and SD (standard deviation) of 0.003. We observed that CTEu complex provides a wider dynamic concentration-range for LDL determination than that from Eu-tetracycline previously. The averaged emission lifetimes of the CTEu and CTEu with LDL (1.5 mg/mL) complexes were measured as 15 and 46 micros, respectively. Study with some metallic interferents is presented.
authors.affiliation: Centro de Lasers e Aplicações, IPEN/CNEN-SP, Brazil.
authors.forename: Luciane Dos Santos
authors.initials: Ldos S
authors.lastname: Teixeira
authors.forename: Andréa Nastri
authors.initials: AN
authors.lastname: Grasso
authors.forename: Andrea Moreira
authors.initials: AM
authors.lastname: Monteiro
authors.forename: A M Figueiredo
authors.initials: AM
authors.lastname: Neto
authors.forename: Nilson Dias
authors.initials: ND
authors.lastname: Vieira
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
authors.forename: Lilia Coronato
authors.initials: LC
authors.lastname: Courrol
mesh_terms: D015374:Biosensing Techniques; D002751:Chlortetracycline / Q000737:chemistry*; D008078:Cholesterol, LDL / Q000032:analysis*; D005063:Europium / Q000737:chemistry*; D006801:Humans; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
chemical_list: D008078:Cholesterol, LDL; D005063:Europium; D002751:Chlortetracycline