title: Portuguese winemaking residues as a potential source of natural anti-adenoviral agents.
abstract: To date there are no licensed systemic or topical treatments in Europe or the USA for adenovirus infections. In the present paper, we evaluate the effect of a polyphenol-based grape extract (NE) obtained from Portuguese white-winemaking by-products, and Resveratrol in pure form, on adenovirus type 5 infection. For this purpose, recombinant adenovirus vectors (Ad-5) and a human-derived cell line (293) were used as models. The NE and Resveratrol at the used concentrations do not induce cell cytotoxicity or direct virucidal activity; however, they reduce 4.5 and 6.5 log (TCID(50)/ml) on total infectious Ad-5 production, respectively. The capacity of Ad-5 replication upon removal of NE and Resveratrol after 24 h post infection was also evaluated. In contrast to Resveratrol, the highest evaluated NE concentration inhibits irreversibly the Ad-5 replication. These results provide useful information for the use of NE and Resveratrol as potential sources of promising natural antiviral agents on Ad-5 infection.
authors.affiliation: Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta do Marquês, Estação Agronómica Nacional, Oeiras, Portugal.
authors.forename: Ana A
authors.initials: AA
authors.lastname: Matias
authors.forename: Ana Teresa
authors.initials: AT
authors.lastname: Serra
authors.forename: A Carina
authors.initials: AC
authors.lastname: Silva
authors.forename: Ricardo
authors.initials: R
authors.lastname: Perdigão
authors.forename: Tiago B
authors.initials: TB
authors.lastname: Ferreira
authors.forename: I
authors.initials: I
authors.lastname: Marcelino
authors.forename: Sandra
authors.initials: S
authors.lastname: Silva
authors.forename: Ana V
authors.initials: AV
authors.lastname: Coelho
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alves
authors.forename: Catarina M M
authors.initials: CM
authors.lastname: Duarte
mesh_terms: D000256:Adenoviridae / Q000187:drug effects* / Q000502:physiology; D000258:Adenovirus Infections, Human / Q000188:drug therapy*; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use; D019649:Food Industry; D005638:Fruit; D005822:Genetic Vectors; D006801:Humans; D007220:Industrial Waste; D010636:Phenols / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D059808:Polyphenols; D011174:Portugal; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D014779:Virus Replication / Q000187:drug effects; D027843:Vitis / Q000737:chemistry*; D014920:Wine
chemical_list: D000998:Antiviral Agents; D005419:Flavonoids; D007220:Industrial Waste; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol