title: [Investigation epidemiology and sub-clinical infection of hepatitis E in voluntary blood donors in south Zhejiang].
abstract: OBJECTIVE
  To investigate the southern region of Zhejiang hepatitis E virus (HEV) infection.
  
  
  METHODS
  A cluster sampling strategy was used to sample all blood donors from February to October in 2008 in Wenzhou blood center. Their blood was tested for IgG and IgM antibody against HEV. Reverse transcriptase-polymerase chain reaction(RT-PCR) and sequencing were applied to detect its genotype and sequence homology in HEV IgM-positive specimen.
  
  
  RESULTS
  The prevalence of anti-HEV IgG in 3044 cases of blood donors was 33.28%. IgG increased with age. There are certain increase in positive rates between the 20-year-old group and over 40 years of age group from 21.16% to 50.36%. The positive rate of IgM was 0.92%. The ratio of infection among different age group was the highest in the age range from 31 to 40 years and up to 1.90%. IgG and IgM through their negative and positive analysis of samples found in their group with donors age, sex and blood type does not significantly related to each other. Nucleic acids were found in three cases through PCR amplification in all 28 cases of HEV IgM positive samples. The total positive rate was one-thousandth, of which two cases for gene 4, 1 cases of infection for gene 1.
  
  
  CONCLUSIONS
  The results indicate that there was a certain percentage of HEV virus in voluntary blood donors in south Zhejiang.
authors.affiliation: Wenzhou blood center, Wenzhou 325001, China. wzxzzgk@126.com
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Huang
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Wang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Yan-Chai
authors.initials: YC
authors.lastname: Tu
authors.forename: Jian-Dao
authors.initials: JD
authors.lastname: Li
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Sheng-Xiang
authors.initials: SX
authors.lastname: Ge
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood; D016751:Hepatitis E / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D055815:Young Adult
chemical_list: D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M