[Effects of intermittent hypoxic exposure on the parameter of erythrocyte and serum hypoxia inducible factor-1 alpha and erythropoietin levels].
OBJECTIVE
To investigate the effects of intermittent hypoxic exposure and normoxic convalescence on the parameter of erythrocyte and serum hypoxia inducible factor-1 alpha (HIF-1alpha) and erythropoietin (EPO) levels.


METHODS
Rat models of intermittent hypoxic exposure were established, combined with the clinical research on volunteers experiencing the intermittent plateau work. Blood samples for red blood cell (RBC) counts, hemoglobin (Hb) and hematocrit (HCT) were collected, serum HIF-1alpha and EPO levels were measured using enzyme linked immunosorbent assay.


RESULTS
RBC counts, Hb concentration and HCT were significantly higher than the normoxic group (P < 0.05), after exposure of rats to hypoxia from 7 to 28 days. Compared with the normoxic group, serum HIF-1alpha levels were higher in the group of IH3, 7, 14 days, and EPO had a corresponding increase in the group of IH3, 7 days. Then, a decrease was observed in parameter of erythrocyte and serum HIF-1alpha and EPO levels after 14 days normoxic convalescence treat. In volunteers studies, RBC counts in 8 months group and Hb concentration in 2 years group were significantly higher than the plain group (P < 0.05). Compared with the plain group, EPO had no significant differences in any of plateau group.


CONCLUSIONS
Intermittent hypoxic exposure can enhance serum hypoxia inducible factor-1 alpha and erythropointin levels and the generation of red blood cells, which leads to an increase in hemoglobin concentration and hematocrit. The results have changed with the hypoxic exposure period prolonged. Normoxic convalescence after intermittent hypoxic exposure can make the related indexes reduced, and contribute to the organism recovery.
Zhang
Cheng-yan
CY
Department of Pathology, Beijing Shijitan Hospital, the Ninth Hospital of Peking University, Beijing, China. zhangchy1862@163.com
Zhang
Ji-xin
JX
Lü
Xiao-tao
XT
Li
Bao-yu
BY
D000293:Adolescent; D000328:Adult; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D004921:Erythropoietin / Q000097:blood*; D006801:Humans; D000860:Hypoxia / Q000097:blood*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D055815:Young Adult
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004921:Erythropoietin