title: [Biodiversity of Lactobacilli from fermented milk products in Northern Tibet].
abstract: OBJECTIVE
  In order to study the biodiversity of Lactobacilli from Northern Tibet in China, we developed enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR) technique.
  
  
  METHODS
  Seventy seven Lactobacillus strains were isolated from traditional fermented milk products which are made by herd man families in Northern Tibet of China. By ERIC-PCR technique method, using NTSYS-pc2.1 software, we investigated the biodiversity of the strains.
  
  
  RESULTS
  The amplified bands of ERIC-PCR were clear and stable with high repetition and high polymorphisms. The cluster analysis showed that at level 0.73, 77 tested strains were clustered into 4 groups: A1: strains of Lactobacillus casei (35.06% of the strains), A2: strains of Lactobacillus fermentum (61.04% of the strains), A3: strains of Lactobacillus helveticus and A4: strains of Lactobacillus plantarum. The biodiversity of the isolates from Aland A2 groups was further investigated. A1 could be divided into 5 genotypes at the similarity of 80.0%. A2 could be divided into 6 genotypes at the similarity of 84.0%.
  
  
  CONCLUSIONS
  Using ERIC-PCR technique, Lactobacilli from Northern Tibet could be distinguished at species level.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China. jn2005282@sina.com
authors.forename: Ning
authors.initials: N
authors.lastname: Ju
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Sun
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.lastname: YeJin
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
authors.forename: Tiansong
authors.initials: T
authors.lastname: Sun
mesh_terms: D044822:Biodiversity; D002681:China; D043302:Cultured Milk Products / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018609:Tibet