title: Tn5090-like class 1 integron carrying bla(VIM-2) in a Pseudomonas putida strain from Portugal.
abstract: Three Pseudomonas putida strains containing bla(VIM-2) were isolated from an inanimate surface of a female ward sanitary facility in the Hospital Infante D. Pedro, Aveiro. A novel class 1 integron was found in strain Pp2 (aacA4/bla(VIM-2)/aac6'-IIc disrupted by an insertion sequence IS1382), and strain Pp1 was found to carry a class 1 integron (aacA7/bla(VIM-2)/aacC1/aacA4), which is described for the first time in this species. Strain PF1 carries a class 1 integron associated with a Tn5090-like transposon, constituting the first finding of this type of arrangement in a strain from Portugal. This association highlights further dissemination of bla(VIM-2) in environmental hospital isolates.
authors.affiliation: CESAM and Department of Biology, University of Aveiro, Campus de Santiago, Aveiro, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Santos
authors.forename: T
authors.initials: T
authors.lastname: Caetano
authors.forename: S
authors.initials: S
authors.lastname: Ferreira
authors.forename: S
authors.initials: S
authors.lastname: Mendo
mesh_terms: D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005260:Female; D023061:Gene Order; D006761:Hospitals; D006801:Humans; D032023:Integrons; D008969:Molecular Sequence Data; D011174:Portugal; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases