Trypanosoma cruzi-specific immune responses in subjects from endemic areas of Chagas disease of Argentina.
Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-gamma ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-gamma responses were detected in 42% of subjects evaluated (15/26 positive by conventional serology and 22/62 seronegative subjects). Using immunoblotting, T. cruzi-specific IgG reactivity was detected in all seropositive subjects and in 11% (7/61) of subjects negative by conventional serology. Measurements of T cell responses and antibodies by immunoblotting, in conjunction with conventional serology, might enhance the capability of detection of exposure to T. cruzi in endemic areas.
Olivera
Gabriela C
GC
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, Buenos Aires 1063, Argentina.
Albareda
Maria C
MC
Alvarez
Maria G
MG
De Rissio
Ana M
AM
Fichera
Laura E
LE
Cooley
Gretchen
G
Yachelini
Pedro
P
Hrellac
Hugo A
HA
Riboldi
Hilda
H
Laucella
Susana A
SA
Tarleton
Rick L
RL
Postan
Miriam
M
D000328:Adult; D000368:Aged; D000913:Antibodies, Protozoan / Q000097:blood; D001118:Argentina; D014355:Chagas Disease / Q000276:immunology*; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007371:Interferon-gamma