Spatiotemporal characterization of phagocytic NADPH oxidase and oxidative destruction of intraphagosomal organisms in vivo using autofluorescence imaging and Raman microspectroscopy.
The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.
Chang
Wei-Tien
WT
National Taiwan University College of Medicine and Hospital, Taipei, Taiwan.
Yang
Yi-Cyun
YC
Lu
Hsueh-Han
HH
Li
I-Lin
IL
Liau
Ian
I
D002470:Cell Survival; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D019255:NADPH Oxidases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013997:Time Factors
D017382:Reactive Oxygen Species; D019255:NADPH Oxidases