Ru(II) glycodendrimers as probes to study lectin-carbohydrate interactions and electrochemically measure monosaccharide and oligosaccharide concentrations.
We describe a novel platform on which to study carbohydrate-protein interactions based on ruthenium(II) glycodendrimers as optical and electrochemical probes. Using the prototypical concanavalin A (ConA)-mannose lectin-carbohydrate interaction as an example, oligosaccharide concentrations were electrochemically monitored. The displacement of the Ru(II) complex from lectin-functionalized gold surfaces was repeatedly regenerated. This new platform presents a method to monitor many different complex sugars in parallel.
Kikkeri
Raghavendra
R
Laboratory for Organic Chemistry, ETH Zurich, Switzerland.
Kamena
Faustin
F
Gupta
Tarkeshwar
T
Hossain
Laila H
LH
Boonyarattanakalin
Siwarutt
S
Gorodyska
Ganna
G
Beurer
Eva
E
Coullerez
Géraldine
G
Textor
Marcus
M
Seeberger
Peter H
PH
D015082:2,2'-Dipyridyl / Q000737:chemistry; D050260:Carbohydrate Metabolism; D003208:Concanavalin A / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D004563:Electrochemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D009005:Monosaccharides / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D040081:Protein Array Analysis; D012428:Ruthenium / Q000737:chemistry*
D050091:Dendrimers; D005456:Fluorescent Dyes; D037102:Lectins; D009005:Monosaccharides; D009844:Oligosaccharides; D003208:Concanavalin A; D015082:2,2'-Dipyridyl; D006046:Gold; D012428:Ruthenium; D008358:Mannose