title: [Experimental research of the inhibition of vascular endothelial growth factor C expression in gastric cancer by targeting RNA interference].
abstract: OBJECTIVE
  To construct the plasmid expression vector pSIH1-H1-copGFP for RNA interference against vascular endothelial growth factor C (VEGF-C) and to evaluate its effect on the expression of VEGF-C mRNA in gastric cancer cells after transfection.
  
  
  METHODS
  Three siRNAs of genome sequence of VEGF-C gene were retrieved from GenBank and one negative chain was used as control. Four siRNAs were cloned into plasmid pSIH1-H1-copGFP,which were then transfected into gastric cancer cells (SGC7901). The expression of VEGF-C mRNA was analyzed by RT-PCR.
  
  
  RESULTS
  The recombinant plasmid of pSIH1-H1-copGFP specific for VEGF-C was confirmed by gene sequencing analysis. The target sequence obtained was completely consistent with the design. Transfection efficiency of the three siRNAs ranged from 60% to 70%. After transfection, the expression of VEGF-C mRNA in SGC7901 cells was significantly inhibited. Inhibition rates of VEGF-C mRNA expression were 35.4%, 33.8% and 81.5% in the three siRNA plasmid vectors, respectively.
  
  
  CONCLUSIONS
  The siRNA expression plasmid vector against VEGF-C mRNA is successfully constructed, and RNAi may be a useful technique to inhibit the lymphangiogenesis of gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Shanghai East Hospital Affiliated to Tongji University, Shanghai 200120, China. guotingchen@163.com
authors.forename: Guo-Ting
authors.initials: GT
authors.lastname: Chen
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Niu
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Sheng-Chao
authors.initials: SC
authors.lastname: Ji
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Han
authors.forename: Yang-Zhou
authors.initials: YZ
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Duan
authors.initials: D
authors.lastname: Cai
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D042582:Vascular Endothelial Growth Factor C