Controlling the particle size of interpolymer complexes through host-guest interaction for drug delivery.
A new method to adjust the particle size of interpolymer complexes has been developed by introduction of host-guest interaction into the dilute aqueous solution of poly(acrylic acid) (PAA) and poly(ethylene glycol) (PEG). Because of the cooperative hydrogen-bonding interaction, PAA can form the interpolymer complexes with PEG. Putting beta-cyclodextrin (beta-CD) into dilute PAA/PEG aqueous solution, the competition between host-guest and hydrogen-bonding interactions happens. The beta-CD/PAA/PEG ternary systems have been well characterized by ultraviolet-visible absorption spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), diffusion NMR spectroscopy, attenuated total reflectance-Fourier transform infrared (ATR-FTIR), and solid-state (13)C NMR spectroscopy. The results indicate that the hydrophobic cavity of beta-CD is threaded by linear polymers so that the hydrophilicity of PAA/PEG interpolymer complexes is improved greatly. Adjusting the amounts of beta-CD, the particle size of the interpolymer complexes can be readily controlled. The low cytotoxicity of various beta-CD/PAA/PEG ternary complexes has been confirmed using the MTT assay in COS-7 cell line. Doxorubicin (DOX), an anticancer drug, has been encapsulated into the beta-CD/PAA/PEG ternary complexes. The DOX-loaded beta-CD/PAA/PEG ternary complexes have been analyzed by confocal laser scanning microscopy (CLSM), flow cytometry analysis, and the MTT assay against human cervical carcinoma cell (Hela). The results indicate that beta-CD/PAA/PEG ternary complexes with controlled particle size could be used as safe and promising drug carriers.
Chen
Yan
Y
School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
Pang
Yan
Y
Wu
Jieli
J
Su
Yue
Y
Liu
Jinyao
J
Wang
Ruibin
R
Zhu
Bangshang
B
Yao
Yefeng
Y
Yan
Deyue
D
Zhu
Xinyuan
X
Chen
Qun
Q
D016503:Drug Delivery Systems; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D011108:Polymers