Prime-boost immunization using a DNA vaccine delivered by attenuated Salmonella enterica serovar typhimurium and a killed vaccine completely protects chickens from H5N1 highly pathogenic avian influenza virus.
H5N1 highly pathogenic avian influenza virus (HPAIV) has posed a great threat not only for the poultry industry but also for human health. However, an effective vaccine to provide a full spectrum of protection is lacking in the poultry field. In the current study, a novel prime-boost vaccination strategy against H5N1 HPAIV was developed: chickens were first orally immunized with a hemagglutinin (HA) DNA vaccine delivered by attenuated Salmonella enterica serovar Typhimurium, and boosting with a killed vaccine followed. Chickens in the combined vaccination group but not in single vaccination and control groups were completely protected against disease following H5N1 HPAIV intranasal challenge, with no clinical signs and virus shedding. Chickens in the prime-boost group also generated significantly higher serum hemagglutination inhibition (HI) titers and intestinal mucosal IgA titers against avian influenza virus (AIV) and higher host immune cellular responses than those from other groups before challenge. These results demonstrated that the prime-boost vaccination strategy provides an effective way to prevent and control H5N1 highly pathogenic avian influenza virus.
Pan
Zhiming
Z
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Zhang
Xiaoming
X
Geng
Shizhong
S
Fang
Qiang
Q
You
Meng
M
Zhang
Lei
L
Jiao
Xinan
X
Liu
Xiufan
X
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D002645:Chickens; D005822:Genetic Vectors; D006385:Hemagglutination Inhibition Tests; D007117:Immunization, Secondary / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000008:administration & dosage; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage; D017201:Virus Shedding / Q000276:immunology
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated