title: PINK1 is selectively stabilized on impaired mitochondria to activate Parkin.
abstract: Loss-of-function mutations in PINK1 and Parkin cause parkinsonism in humans and mitochondrial dysfunction in model organisms. Parkin is selectively recruited from the cytosol to damaged mitochondria to trigger their autophagy. How Parkin recognizes damaged mitochondria, however, is unknown. Here, we show that expression of PINK1 on individual mitochondria is regulated by voltage-dependent proteolysis to maintain low levels of PINK1 on healthy, polarized mitochondria, while facilitating the rapid accumulation of PINK1 on mitochondria that sustain damage. PINK1 accumulation on mitochondria is both necessary and sufficient for Parkin recruitment to mitochondria, and disease-causing mutations in PINK1 and Parkin disrupt Parkin recruitment and Parkin-induced mitophagy at distinct steps. These findings provide a biochemical explanation for the genetic epistasis between PINK1 and Parkin in Drosophila melanogaster. In addition, they support a novel model for the negative selection of damaged mitochondria, in which PINK1 signals mitochondrial dysfunction to Parkin, and Parkin promotes their elimination.
authors.affiliation: Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Derek P
authors.initials: DP
authors.lastname: Narendra
authors.forename: Seok Min
authors.initials: SM
authors.lastname: Jin
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tanaka
authors.forename: Der-Fen
authors.initials: DF
authors.lastname: Suen
authors.forename: Clement A
authors.initials: CA
authors.lastname: Gautier
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Mark R
authors.initials: MR
authors.lastname: Cookson
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Youle
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D045726:Metalloproteases / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology; D051336:Mitochondrial Membranes / Q000378:metabolism; D008954:Models, Biological; D010300:Parkinson Disease / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D000099202:PTEN-Induced Putative Kinase
chemical_list: D045726:Metalloproteases; D011494:Protein Kinases; D044767:Ubiquitin-Protein Ligases; D000099202:PTEN-Induced Putative Kinase; C111567:parkin protein