title: Production of xylanolytic enzymes by Penicillium janczewskii.
abstract: The production of extracellular xylanase, beta-xylosidase and alpha-l-arabinofuranosidase by the mesophilic fungus Penicillium janczewskii under submerged cultivation was investigated with different carbon sources. Optimization steps included studies of carbon source concentration, temperature of cultivation and initial pH of culture medium. The production of these enzymes was increased two times when cultures were supplemented with brewer's spent grain at 2% concentration, pH 6.0 and carried out at 25 degrees C. Under these optimized conditions were obtained xylanase activity of 15.19UmL(-1) and 23.54Umgprot(-1), beta-xylosidase activity of 0.16UmL(-1) and 0.25Umgprot(-1) and alpha-l-arabinofuranosidase activity of 0.67UmL(-1) and 1.04Umgprot(-1). Brewer's spent grain is a promising substrate for P. janczewskii growth and xylanolytic enzyme production, since it is the main by-product from the brewing industry, available in large amounts and at low-cost in many countries.
authors.affiliation: Biochemistry and Microbiology Department, Biosciences Institute, UNESP, São Paulo State University, PO 155, Rio Claro, SP, CEP 13506-900, Brazil. ecarmona@rc.unesp.br
authors.forename: César Rafael Fanchini
authors.initials: CR
authors.lastname: Terrasan
authors.forename: Beatriz
authors.initials: B
authors.lastname: Temer
authors.forename: Marta Cristina Teixeira
authors.initials: MC
authors.lastname: Duarte
authors.forename: Eleonora Cano
authors.initials: EC
authors.lastname: Carmona
mesh_terms: D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000201:enzymology*; D013696:Temperature; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D014990:Xylans; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase