title: A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. extralong autumn purple bean.
abstract: Lectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV-1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Evandro Fei
authors.initials: EF
authors.lastname: Fang
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.forename: Jack Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Sai Wah
authors.initials: SW
authors.lastname: Tsao
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D007887:Fabaceae / Q000737:chemistry*; D054303:HIV Reverse Transcriptase / Q000302:isolation & purification*; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000235:genetics; D027805:Phaseolus / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons
chemical_list: D004791:Enzyme Inhibitors; D037102:Lectins; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II; D054303:HIV Reverse Transcriptase