title: Surrobodies with functional tails.
abstract: Surrobodies(2) are a unique type of binding protein based on the pre-B-cell receptor (pre-BCR). The pre-BCR is transiently expressed during development of the antibody repertoire. Unlike heterotetrameric canonical antibodies that are composed of identical pairs of heavy and light chains, the pre-BCR is a heterohexameric complex composed of identical pairs of heavy chains that are each paired with a two-subunit surrogate light chain (SLC). The SLC contains nonimmunoglobulin-like peptide extensions on each of the two SLC components. This arrangement provides unique opportunities for protein engineering by functional derivatization of these nonimmunoglobulin-like tails. Here we report recombinant fusions to these tails with either a fully active cytokine or with single-chain variable fragment (scFv) domains to generate Surrobodies with unique functions or Surrobodies that are bispecific with respect to targeted binding.
authors.affiliation: Sea Lane Biotechnologies, 1455 Adams Drive, Menlo Park, CA 94025, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Angeles
authors.initials: A
authors.lastname: Estellés
authors.forename: Raffaella
authors.initials: R
authors.lastname: Briante
authors.forename: Aaron L
authors.initials: AL
authors.lastname: Kurtzman
authors.forename: Charles H
authors.initials: CH
authors.lastname: Hannum
authors.forename: Arun K
authors.initials: AK
authors.lastname: Kashyap
authors.forename: Lawrence
authors.initials: L
authors.lastname: Horowitz
authors.forename: Michael
authors.initials: M
authors.lastname: Horowitz
authors.forename: Ramesh R
authors.initials: RR
authors.lastname: Bhatt
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000096:biosynthesis; D016466:CHO Cells; D036022:Capsid Proteins / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D054444:Immunoglobulin Light Chains, Surrogate / Q000737:chemistry* / Q000235:genetics; D007376:Interleukin-2 / Q000378:metabolism; D015202:Protein Engineering; D021122:Protein Subunits / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D018033:Antibodies, Bispecific; D036022:Capsid Proteins; D054444:Immunoglobulin Light Chains, Surrogate; D007376:Interleukin-2; D021122:Protein Subunits; D018121:Receptors, Cytokine; D011993:Recombinant Fusion Proteins