Lapachol induces clastogenic effects in rats.
Lapachol is a naturally occurring naphthoquinone derivative found in the heartwood of several plants, particularly those of the genus Tabebuia (Bignoneaceae). Despite its use as a therapeutic product with anti-inflammatory, analgesic, antipsoriatic, trypanocidal effects, among others, its in vivo mutagenic potential has still not been investigated. This paper reports the effects after a single oral administration of lapachol in the in vivo micronucleus (MN) and chromosome aberration (CA) assays. Both assays were performed using bone marrow cells from male Wistar rats. The animals were treated by oral gavage with hydroalcoholic solutions of lapachol at the doses of 122, 244 and 365 mg/kg, chosen on the basis of the LD(50) in male rats. The results show that the higher administered lapachol dose induced a significant increase in the frequency of micronucleated polychromatic erythrocytes (MNPCE) and CAs in rat bone marrow cells, indicating that lapachol shows clastogenic effects under the experimental conditions used.
Maistro
Edson Luis
EL
Departamento de Fonoaudiologia, Faculdade de Filosofia e Ciências, Universidade Estadual Paulista (UNESP), Marília, São Paulo, Brazil. edson.maistro@marilia.unesp.br
Fernandes
Diego Mota
DM
Pereira
Fernanda Maria
FM
Andrade
Sergio Faloni
SF
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D002869:Chromosome Aberrations; D004912:Erythrocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000009:adverse effects*; D009285:Naphthoquinones / Q000009:adverse effects*; D010936:Plant Extracts / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D029663:Tabebuia / Q000737:chemistry*; D014934:Wood
D009153:Mutagens; D009285:Naphthoquinones; D010936:Plant Extracts; C008252:lapachol