title: The protective effect of ischemic preconditioning associated with altered gene expression profiles in rat lung after reperfusion.
abstract: BACKGROUND
  Ischemia-reperfusion (IR) injury remains an important clinical problem after lung transplantation. It is well-known that ischemic preconditioning (IPC) markedly enhances the ability of organs to withstand a sustained ischemia and reperfusion injury. But the molecular mechanisms of this protective effect are poorly understood. Therefore, we used gene microarrays to profile gene expression patterns involved in ischemia precondition.
  
  
  METHODS
  Three groups of rats were studied: IR group (IR 0, 1, 3, 6, 24, n = 5 each group, IPC group (IP 1, IP 3, 6, 24 h, n = 5 each group) and Sham group (n = 5). Samples were collected from different groups. The RatRef-12 expression Beadchip (22,226 gene probes per array) was used to analyze the pattern of gene expression in all groups.
  
  
  RESULTS
  Microarray analysis showed that 648, 340, 711, 1279, and 641 genes were differentially expressed in IR 0-, 1-, 3-, 6- and 24-h groups, respectively. The profile revealed that IPC induced significant alterations of expression of many genes encoding inflammatory associated factors, oxidation and antioxidant molecules, apoptosis regulatory protein, metabolic enzyme, and ion channel protein.
  
  
  CONCLUSIONS
  The damage and repair after lung IR are a dynamic process. IPC affects gene expression profiles in IR lung tissue mainly within 6 h, and sustains until 24 h later. IPC reduces lung IR injury mainly through anti-inflammatory response, antioxidative stress, and regulating cell energy metabolism and ion channels.
authors.affiliation: Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Nie
authors.initials: N
authors.lastname: Jun
authors.forename: Jiang
authors.initials: J
authors.lastname: Ke
authors.forename: Cheng
authors.initials: C
authors.lastname: Gang
authors.forename: Cheng
authors.initials: C
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Jinsong
authors.forename: Wang
authors.initials: W
authors.lastname: Jianjun
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D007511:Ischemia / Q000378:metabolism*; D019194:Ischemic Preconditioning; D008168:Lung / Q000378:metabolism*; D055370:Lung Injury / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D012313:RNA