title: Vitreous levels of interleukine-8 and monocyte chemoattractant protein-1 in macular oedema with branch retinal vein occlusion.
abstract: OBJECTIVE
  To investigate whether interleukine-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are related with macular oedema in patients with branch retinal vein occlusions (BRVOs).
  
  
  METHODS
  Retrospective case-control study.
  
  
  METHODS
  Nineteen patients who had macular oedema due to BRVO and nine patients with non-ischaemic ocular diseases (control group).
  
  
  METHODS
  Macular oedema was examined by optical coherence tomography. Both venous blood and vitreous samples were obtained at the time of vitreoretinal surgery. IL-8 and MCP-1 levels in vitreous fluid and plasma were determined with enzyme-linked immunosorbent assay kits. Variables were compared with the Mann-Whitney U-test, Wilcoxon's signed-ranked test, and the chi2-test, when appropriate. To examine correlations, Spearman's rank-order correlation coefficients were calculated. Statistical significance was set at P<0.05.
  
  
  RESULTS
  The vitreous fluid levels of IL-8 (median: 63.5 pg/ml) and MCP-1 (median: 1522.4 pg/ml) were significantly higher in the patients with BRVO than in the control group (median: 5.1 and 746.5 pg/ml respectively; P<0.001 and <0.001 respectively). Vitreous IL-8 and MCP-1 were significantly correlated in patients with BRVO (P=0.009).
  
  
  CONCLUSIONS
  Both IL-8 and MCP-1 were elevated in the vitreous fluid of patients with BRVO and macular oedema. Both chemokines may contribute to the pathogenesis of macular oedema in patients with BRVO.
authors.affiliation: Department of Ophthalmology, Hospital Valle Hebron de Barcelona, Barcelona, Spain. 36427afc@comb.es
authors.forename: A
authors.initials: A
authors.lastname: Fonollosa
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Arumi
authors.forename: E
authors.initials: E
authors.lastname: Santos
authors.forename: C
authors.initials: C
authors.lastname: Macia
authors.forename: P
authors.initials: P
authors.lastname: Fernandez
authors.forename: R M
authors.initials: RM
authors.lastname: Segura
authors.forename: M A
authors.initials: MA
authors.lastname: Zapata
authors.forename: R
authors.initials: R
authors.lastname: Rodriguez-Infante
authors.forename: A
authors.initials: A
authors.lastname: Boixadera
authors.forename: V
authors.initials: V
authors.lastname: Martinez-Castillo
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D054426:Chemokine CXCL2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000378:metabolism*; D008269:Macular Edema / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012170:Retinal Vein Occlusion / Q000150:complications / Q000378:metabolism*; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D054426:Chemokine CXCL2; D016209:Interleukin-8