title: Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells.
abstract: OBJECTIVE
  To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering.
  
  
  METHODS
  New Zealand rabbits (n = 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles.
  
  
  RESULTS
  The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC.
  
  
  CONCLUSIONS
  The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.
authors.affiliation: Department of Plastic Surgery, Lusiada University of Santos, Sao Paulo, Brazil.
authors.forename: Marcelo Paulo Vaccari
authors.initials: MP
authors.lastname: Mazzetti
authors.forename: Isis Sousa
authors.initials: IS
authors.lastname: Oliveira
authors.forename: Regiane
authors.initials: R
authors.lastname: Miranda-Ferreira
authors.forename: Grasiele
authors.initials: G
authors.lastname: Fauaz
authors.forename: Chaibe Nunes
authors.initials: CN
authors.lastname: Ribeiro
authors.forename: Paulo Oliveira
authors.initials: PO
authors.lastname: Gomes
authors.forename: Paulo
authors.initials: P
authors.lastname: Pontes
authors.forename: Alice Teixeira
authors.initials: AT
authors.lastname: Ferreira
authors.forename: Lilian Piñero
authors.initials: LP
authors.lastname: Eça
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000601:surgery; D000818:Animals; D002469:Cell Separation; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023421:Models, Animal; D011817:Rabbits