title: [Study on extraction and purification process of total ginsenosides from Radix Ginseng].
abstract: OBJECTIVE
  To optimize the technological parameters of the extraction and purification process of total ginsenosides from Radix Ginseng.
  
  
  METHODS
  With the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1, the orthogonal design was adopted to optimize the extraction process. The purification process was studied by optimizing the elutive ratio of total ginsenosides as the marker. HPLC and spectrophotometer were employed for the study.
  
  
  RESULTS
  The optimum conditions were as follows:Using 8 times volume of 75% ethanol extracting for 120 minutes and 2 times, the extraction temperature was 85 degrees C. AB-8 macroporous resin was selected, and the eluant was 4 BV 70% ethanol.
  
  
  CONCLUSIONS
  The optimal conditions of extracting and purifying the total ginsenosides from Radix Ginseng is feasible.
authors.affiliation: Department of Biology, Shantou University, Shantou 515063, China. llxie@stu.edu.cn
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Lai
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Cao
authors.forename: Quan-Yi
authors.initials: QY
authors.lastname: Mo
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D036145:Ginsenosides / Q000032:analysis / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012117:Resins, Synthetic; D012997:Solvents; D000431:Ethanol; C049864:ginsenoside Re; C035054:ginsenoside Rg1