title: Quantification of fibrosis and osteosclerosis in myeloproliferative neoplasms: a computer-assisted image study.
abstract: Evaluation of bone marrow fibrosis and osteosclerosis in myeloproliferative neoplasms (MPN) is subject to interobserver inconsistency. Performance data for currently utilized fibrosis grading systems are lacking, and classification scales for osteosclerosis do not exist. Digital imaging can serve as a quantification method for fibrosis and osteosclerosis. We used digital imaging techniques for trabecular area assessment and reticulin fiber quantification. Patients with all Philadelphia negative MPN subtypes had higher trabecular volume than controls (p<or=0.0015). Results suggest that the degree of osteosclerosis helps differentiate primary myelofibrosis from other MPN. Numerical quantification of fibrosis highly correlated with subjective scores, and interobserver correlation was satisfactory. Digital imaging provides accurate quantification for osteosclerosis and fibrosis.
authors.affiliation: Department of Pathology, University of Utah, Salt Lake City, UT 84108, USA.
authors.forename: Carolin J
authors.initials: CJ
authors.lastname: Teman
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Wilson
authors.forename: Sherrie L
authors.initials: SL
authors.lastname: Perkins
authors.forename: Kimberly
authors.initials: K
authors.lastname: Hickman
authors.forename: Josef T
authors.initials: JT
authors.lastname: Prchal
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Salama
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D001856:Bone Marrow Examination / Q000379:methods*; D004252:DNA Mutational Analysis; D005260:Female; D005355:Fibrosis; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D053614:Janus Kinase 2 / Q000235:genetics; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000145:classification / Q000150:complications / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D015588:Observer Variation; D010026:Osteosclerosis / Q000175:diagnosis / Q000209:etiology / Q000473:pathology*; D053628:Receptors, Thrombopoietin / Q000235:genetics; D015203:Reproducibility of Results; D012155:Reticulin / Q000032:analysis*; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D053628:Receptors, Thrombopoietin; D012155:Reticulin; C082218:MPL protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2