title: Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach.
abstract: Larval nematodes were collected from marine fishes from the Yellow Sea, China. Specimens (n=1731) of Anisakis type I from 311 fishes (representing 40 species) were each identified based on morphological characters. From the genomic DNA from individual specimens, a region of nuclear ribosomal DNA was amplified by PCR, followed by digestion with restriction endonuclease HinfI, TaqI or HhaI. Subsequently, the ITS-1 and ITS-2 regions of selected samples were sequenced. The results revealed three species of Anisakis, namely Anisakis pegreffii (n=1709), A. typica (n=3) and a genotype (n=19) proposed, also based on comparison with previous studies, to be a "hybrid" between A. pegreffii and A. simplex sensu stricto. Thus, A. pegreffii was the dominant species, accounting for 98.7% of the total number of specimens examined herein. This is the first report of A. typica and the "hybrid" genotype from fishes from the Yellow Sea. This study provides important basic information on Anisakis in this region and suggests that the genus Anisakis has substantial host and geographical distributions.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang, Hebei Province 050016, P R China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Du
authors.forename: Luping
authors.initials: L
authors.lastname: Zhang
authors.forename: Meiqing
authors.initials: M
authors.lastname: Shi
authors.forename: Zhu
authors.initials: Z
authors.lastname: Ming
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D017163:Anisakis / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005399:Fishes / Q000469:parasitology*; D007814:Larva / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D021903:DNA, Ribosomal Spacer