title: Detection of dengue virus in saliva and urine by real time RT-PCR.
abstract: Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto14040-903, São Paulo, Brasil.
authors.forename: Telma R
authors.initials: TR
authors.lastname: Poloni
authors.forename: Anibal S
authors.initials: AS
authors.lastname: Oliveira
authors.forename: Helda L
authors.initials: HL
authors.lastname: Alfonso
authors.forename: Larissa R
authors.initials: LR
authors.lastname: Galvão
authors.forename: Alberto A
authors.initials: AA
authors.lastname: Amarilla
authors.forename: Dimair F
authors.initials: DF
authors.lastname: Poloni
authors.forename: Luiz T
authors.initials: LT
authors.lastname: Figueiredo
authors.forename: Victor H
authors.initials: VH
authors.lastname: Aquino
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000821:virology*; D044967:Serum / Q000821:virology; D014556:Urine / Q000821:virology*
chemical_list: D012367:RNA, Viral