[Epitope screening of influenza A (H3N2) by using phage display library].
OBJECTIVE
To screen the influenza A (H3N2) mimotopes by using phage display library.


METHODS
Using influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.


RESULTS
21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2).


CONCLUSIONS
Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.
Zhong
Yan-Wei
YW
Viral hepatitis research laboratary, Institute of Infectious Disease of P1A 302 Hospital, Beijing 100039, China.
Xu
Dong-Ping
DP
Li
Xiao-Dong
XD
Dai
Jiw-Zeng
JZ
Xu
Biao
B
Li
Le
L
D018604:Epitope Mapping; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library
D019151:Peptide Library