title: Extremely low frequency magnetic field exposure affects DnaK and GroEL expression in E. coli cells with impaired heat shock response.
abstract: In our earlier experiments, we found that extremely low frequency magnetic fields (ELF-MF) affect heat shock protein (HSP) expression in wild type Escherichia coli cells. In the present work we investigate the ability of ELF-MF exposure to trigger an increase of DnaK and GroEL protein levels also in E. coli cells not exhibiting the classic heat shock response (HSR) when subjected to a 42 degrees C heat stress. We find that these cells, although lacking a HSR to heat shock treatment, show an enhancement of DnaK and GroEL protein levels after 30 or 90 min sinusoidal ELF-MF exposure (50 Hz, 1 mT). This result suggests that the HSP induction pathway triggered by ELF-MF exposure could be different from that elicited by heat shock treatment.
authors.affiliation: Department of Evolutionary Experimental Biology, University of Bologna, via Selmi 3, 40126 Bologna, Italy.
authors.forename: Brunella
authors.initials: B
authors.lastname: Del Re
authors.forename: Pamela
authors.initials: P
authors.lastname: Marcantonio
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Bersani
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Giorgi
mesh_terms: D018834:Chaperonin 60 / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D008280:Magnetics; D013696:Temperature
chemical_list: D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; C063842:dnaK protein, E coli