[Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008].
OBJECTIVE
To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene.


METHODS
Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4.


RESULTS
The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain.


CONCLUSIONS
Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.
Li
Ming-Hua
MH
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Fu
Shi-Hong
SH
Feng
Yun
Y
Gao
Xiao-Yan
XY
Zhai
You-Gang
YG
Yu
De-Shan
DS
Li
Guo-Tai
GT
Jia
Yu-Xin
YX
Liang
Guo-Dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002681:China; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins