title: Herbicide formulation with glyphosate affects growth, acetylcholinesterase activity, and metabolic and hematological parameters in piava (Leporinus obtusidens).
abstract: The teleost fish Leporinus obtusidens (piava) was exposed to different concentrations of Roundup, a commercial herbicide formulation containing glyphosate (0, 1, or 5 mg L(-1)), for 90 days. Acetylcholinesterase (AChE) activity was verified in brain and muscle. Hepatic and muscular metabolic parameters as well as some hematological parameters were determined. The results showed that brain AChE activity was significantly decreased in fish exposed to 5 mg L(-1) Roundup, whereas muscular AChE activity was not altered. Both Roundup concentrations significantly decreased liver glycogen without altering the muscle glycogen content. Hepatic glucose levels were reduced only in fish exposed to 5 mg L(-1) Roundup. Lactate levels in the liver and muscle significantly increased in fish exposed to both Roundup concentrations. Hepatic protein content remained constant at 1 mg L(-1) but increased at 5 mg L(-1) Roundup. In the muscle however, protein content decreased with increasing exposure concentration. The herbicide exposure produced a decrease in hematological parameters at both concentrations tested. The majority of observed effects occur at environmental relevant concentrations, and in summary, the results show that Roundup affects brain AChE activity as well as metabolic and hematologic parameters of piavas. Thus, we can suggest that long-term exposure to Roundup causes metabolic disruption in Leporinus obtusidens.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105.900, Santa Maria, RS, Brazil.
authors.forename: Joseânia
authors.initials: J
authors.lastname: Salbego
authors.forename: Alexandra
authors.initials: A
authors.lastname: Pretto
authors.forename: Carolina Rosa
authors.initials: CR
authors.lastname: Gioda
authors.forename: Charlene Cavalheiro
authors.initials: CC
authors.lastname: de Menezes
authors.forename: Rafael
authors.initials: R
authors.lastname: Lazzari
authors.forename: João
authors.initials: J
authors.lastname: Radünz Neto
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
authors.forename: Vania Lucia
authors.initials: VL
authors.lastname: Loro
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D005399:Fishes / Q000097:blood / Q000254:growth & development* / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects; D000097797:Glyphosate
chemical_list: D000110:Acetylcholinesterase; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate