title: Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens.
abstract: We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.
authors.affiliation: Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands.
authors.forename: Jan W M
authors.initials: JW
authors.lastname: van der Linden
authors.forename: Eveline
authors.initials: E
authors.lastname: Snelders
authors.forename: Jan P
authors.initials: JP
authors.lastname: Arends
authors.forename: Simon M
authors.initials: SM
authors.lastname: Daenen
authors.forename: Willem J G
authors.initials: WJ
authors.lastname: Melchers
authors.forename: Paul E
authors.initials: PE
authors.lastname: Verweij
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001230:Aspergillus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D001393:Azoles / Q000494:pharmacology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D017931:DNA Primers; D004271:DNA, Fungal