title: [Antigenic composition and serologic characteristics of domestic acellular pertussis vaccine].
abstract: OBJECTIVE
  To assess antigenic composition consistency and serological characteristics of domestic acellular pertussis vaccine.
  
  
  METHODS
  Amount of pertussis toxin, filamentous hemagglutinin, agglutinogens types 1, 2, and 3 in experimental batches of vaccine was measured by enzyme immunoassay. Levels of antibodies to aforementioned antigens as well as to lipopolysaccbaride in serum samples obtained from patients with pertussis and healthy vaccinated children were measured by the same method. The amount of lypopolysaccharide was determined by LAL test.
  
  
  RESULTS
  Studied batches of vaccine were standard on amount of all protein antigens as well as lipopolysaccharide. Spectrum of antibodies to vaccine components in serum samples from patients with pertussis and healthy vaccinated children included antibodies to individual antigens: pertussis toxin, filamentous hemagglutinin, lipopolysaccharide, agglutinogens types 1, 2, and 3.
  
  
  CONCLUSIONS
  Developed technology for manufacturing acellular pertussis vaccine allows to consistently produce preparations with standard amount of all components. Vaccine components interact with antibodies to wide spectrum of B. pertussis antigens.
authors.forename: E M
authors.initials: EM
authors.lastname: Zaĭtsev
authors.forename: A V
authors.initials: AV
authors.lastname: Poddubikov
authors.forename: M V
authors.initials: MV
authors.lastname: Britsina
authors.forename: N U
authors.initials: NU
authors.lastname: Mertsalova
authors.forename: I G
authors.initials: IG
authors.lastname: Bazhanova
authors.forename: M N
authors.initials: MN
authors.lastname: Ozeretskovskaia
authors.forename: M P
authors.initials: MP
authors.lastname: Nazirov
authors.forename: I K
authors.initials: IK
authors.lastname: Mazurova
authors.forename: M S
authors.initials: MS
authors.lastname: Petrova
authors.forename: O P
authors.initials: OP
authors.lastname: Popova
authors.forename: N S
authors.initials: NS
authors.lastname: Zakharova
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000592:standards*; D006801:Humans; D007223:Infant; D014917:Whooping Cough / Q000097:blood / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines