title: Immature animals have higher cellular density in the healing anterior cruciate ligament than adolescent or adult animals.
abstract: There has been recent interest in the biologic stimulation of anterior cruciate ligament (ACL) healing. However, the effect of age on the ability of ligaments to heal has not yet been defined. In this study, we hypothesized that skeletal maturity would significantly affect the cellular and vascular repopulation rate of an ACL wound site. Skeletally Immature (open physes), Adolescent (closing physes), and Adult (closed physes) Yucatan minipigs underwent bilateral ACL transection and suture repair using a collagen-platelet composite. The response to repair was evaluated histologically at 1, 2, and 4 weeks. All three groups of animals had completely populated the ACL wound site with fibroblasts at 1 week. The Immature animals had a higher cellular density in the wound site than the Adult animals at weeks 2 and 4. Cells in the Immature ligament wounds were larger and more ovoid than in the Adult wounds. There were no significant differences in the vascular density in the wound site. Animal age had a significant effect on the density of cells populating the ACL wound site. Whether this observed cellular difference has an effect on the later biomechanical function of the repaired ACL requires further study.
authors.affiliation: Department of Orthopaedic Surgery, Children's Hospital of Boston, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Ashley N
authors.initials: AN
authors.lastname: Mastrangelo
authors.forename: Brian M
authors.initials: BM
authors.lastname: Haus
authors.forename: Patrick
authors.initials: P
authors.lastname: Vavken
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Palmer
authors.forename: Jason T
authors.initials: JT
authors.lastname: Machan
authors.forename: Martha M
authors.initials: MM
authors.lastname: Murray
mesh_terms: D000375:Aging; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000254:growth & development; D000070598:Anterior Cruciate Ligament Injuries; D002452:Cell Count; D005347:Fibroblasts / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature; D014945:Wound Healing / Q000502:physiology*