title: Detection of nitric oxide in tissue samples by ESI-MS.
abstract: A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chosen as a probe for the detection of NO by mass spectrometry (MS) in biological tissue samples. The o-phenylenediamine group reacts with NO to form a characteristic benzotriazole. The product was identified using electrospray ionization mass spectrometry (ESI-MS) and the method validated within the range of 95-1900 nM. NO levels associated with tissue biopsies (approximately 10 mg) from rat vasculature and intestine tissue biopsies have been successfully determined. The different rates of NO generated from tissue samples under hypoxic and normoxic conditions have been studied by this simple and sensitive method.
authors.affiliation: Department of Chemistry, Cottingham Rd, Hull, UK HU6 7RX.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shen
authors.forename: Abigail
authors.initials: A
authors.lastname: Webster
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Welham
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Dyer
authors.forename: John
authors.initials: J
authors.lastname: Greenman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Haswell
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D000860:Hypoxia; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D009569:Nitric Oxide / Q000032:analysis*; D010655:Phenylenediamines / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010655:Phenylenediamines; D009569:Nitric Oxide; C034193:1,2-diaminobenzene