Identification of pathogenic Vibrio species by multilocus PCR-electrospray ionization mass spectrometry and its application to aquatic environments of the former soviet republic of Georgia.
The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
Whitehouse
Chris A
CA
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MA, USA. chris.whitehouse@us.army.mil
Baldwin
Carson
C
Sampath
Rangarajan
R
Blyn
Lawrence B
LB
Melton
Rachael
R
Li
Feng
F
Hall
Thomas A
TA
Harpin
Vanessa
V
Matthews
Heather
H
Tediashvili
Marina
M
Jaiani
Ekaterina
E
Kokashvili
Tamar
T
Janelidze
Nino
N
Grim
Christopher
C
Colwell
Rita R
RR
Huq
Anwar
A
D001431:Bacteriological Techniques / Q000379:methods*; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D005846:Georgia (Republic); D016133:Polymerase Chain Reaction / Q000379:methods*; D012623:Seawater / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification*
D004269:DNA, Bacterial; D002772:Cholera Toxin