Sealing effect of a polysaccharide nanosheet for murine cecal puncture.
BACKGROUND
Recent developments in nanobiotechnology have led us to develop a method of producing a free-standing polymer nanosheet composed of polysaccharides (ie, polysaccharide nanosheet) with a thickness of tens of nanometers. Owing to its enormous aspect ratio, the polysaccharide nanosheet is semi-absorbent and has a physical adhesive strength 7.5-fold greater than that of conventional films of >1 microm thickness. Herein, we have investigated the therapeutic sealing effect of this polysaccharide nanosheet on murine cecal puncture as a wound dressing material.


METHODS
Murine cecum was punctured and then overlapped with the polysaccharide nanosheet. Thereafter, we evaluated its sealing effect on bacterial peritonitis as well as the protection offered by the polysaccharide nanosheet against bacterial permeability using an in vitro transmembrane assay.


RESULTS
The 39-nm-thick polysaccharide nanosheet overlapped tightly the perforated cecum. No adhering agents were required because of the ability of the polysaccharide nanosheet to adhere to the tissue surface by physical adsorption (eg, van der Waals interaction). Sealing the perforated cecum with the polysaccharide nanosheet increased survival rate without postoperative adhesion by comparison with untreated mice (90 vs 30%; P < .01). These data were supported by the improvement in peritonitis related to bacterial counts, white blood cell counts, and the serum tumor necrosis factor level. Moreover, using an in vitro transmembrane assay, we showed that the polysaccharide nanosheet inhibited effectively bacterial penetration.


CONCLUSIONS
We have demonstrated the potential clinical benefits of the nanosheet-type biomaterial that can be used for repairing a cecal colotomy without chemical bonding agents.
Fujie
Toshinori
T
Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku, Tokyo, Japan.
Kinoshita
Manabu
M
Shono
Satoshi
S
Saito
Akihiro
A
Okamura
Yosuke
Y
Saitoh
Daizoh
D
Takeoka
Shinji
S
D000818:Animals; D018988:Bacterial Translocation; D001458:Bandages; D002432:Cecum; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D049329:Nanostructures; D010538:Peritonitis / Q000382:microbiology / Q000628:therapy*; D010539:Permeability; D011134:Polysaccharides / Q000627:therapeutic use*; D011677:Punctures; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha