title: Identification of pathogenic Vibrio species by multilocus PCR-electrospray ionization mass spectrometry and its application to aquatic environments of the former soviet republic of Georgia.
abstract: The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
authors.affiliation: U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MA, USA. chris.whitehouse@us.army.mil
authors.forename: Chris A
authors.initials: CA
authors.lastname: Whitehouse
authors.forename: Carson
authors.initials: C
authors.lastname: Baldwin
authors.forename: Rangarajan
authors.initials: R
authors.lastname: Sampath
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Blyn
authors.forename: Rachael
authors.initials: R
authors.lastname: Melton
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Hall
authors.forename: Vanessa
authors.initials: V
authors.lastname: Harpin
authors.forename: Heather
authors.initials: H
authors.lastname: Matthews
authors.forename: Marina
authors.initials: M
authors.lastname: Tediashvili
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Jaiani
authors.forename: Tamar
authors.initials: T
authors.lastname: Kokashvili
authors.forename: Nino
authors.initials: N
authors.lastname: Janelidze
authors.forename: Christopher
authors.initials: C
authors.lastname: Grim
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
authors.forename: Anwar
authors.initials: A
authors.lastname: Huq
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D005846:Georgia (Republic); D016133:Polymerase Chain Reaction / Q000379:methods*; D012623:Seawater / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D002772:Cholera Toxin