title: Molecular analysis of mutations for the adenomatous polyposis coli (APC) gene in Romanian patients with colorectal cancer.
abstract: Mutations in adenomatous polyposis coli (APC) gene have not been previously characterized among Romanian patients with colorectal cancer (CRC). We initiate this study to detect the mutations in APC gene in blood and tumor samples collected from 16 patients (10 men and 6 women) and blood samples from 21 first and second degree relatives of the patients. For this the presence of mutations in exons 6, 7, 12, 13, 14 as well as in regions B, L and W of exon 15 was investigated using PCR multiplex. In the same time, we have searched for 5 bp deletions at codon 1061 of APC gene by PAGE and SSCP methods. These methods allowed us to evidence identification of the presence of mutations in samples from 7 individuals. In one patient, was detected a deletion of exon 13th of APC gene both in DNA extracted from blood and tumor samples. Multiple deletions (e.g. in exon 6, 12, and in 15L and 15W regions) in DNA extracted from the tumor sample were detected, but not in DNA probe obtained from blood cells. We can speculate that these mutations are an example of genomic instability accompanying the malignancy. Till now, no mutation affecting 1061 codon of APC gene was identified in the patients investigated in our study.
authors.affiliation: Departamentul de Genetică Umană, Institutul de Genetică, Universitatea din Bucureşti, România. iahim_t@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Toma
authors.forename: D
authors.initials: D
authors.lastname: Cimponeriu
authors.forename: A
authors.initials: A
authors.lastname: Pompilia
authors.forename: M
authors.initials: M
authors.lastname: Stavarachi
authors.forename: L
authors.initials: L
authors.lastname: Beluşică
authors.forename: I
authors.initials: I
authors.lastname: Radu
authors.forename: L
authors.initials: L
authors.lastname: Gavrilă
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000453:epidemiology / Q000235:genetics*; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016368:Frameshift Mutation; D017491:Genes, APC; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D012383:Romania / Q000453:epidemiology
chemical_list: D025601:Adenomatous Polyposis Coli Protein