Libanoridin inhibits the mast cell-mediated allergic inflammatory reaction.
OBJECTIVE
Corydalis heterocarpa is a biennial herb in South Korea, with spikes of yellow flowers. It has been used for as a folk medicine to cure travail and spasm. However, studies on this herb and its secondary metabolites have rarely been reported. In the present study, we isolated secondary metabolite libanlibanoridin from Corydalis heterocarpa. We have also examined the effect of libanoridin on the inflammatory cytokines production in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore, A2318 stimulated human mast cell line, HMC-1. PMA plus A23187 significantly increased interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha production compared to media control (P < 0.05).


RESULTS
We report that treatment with libanlibanoridin can inhibit PMA plus A23187-induced IL-1beta, IL-6, IL-8, and TNF-alpha production in a concentration-dependent manner with IC50 of 0.002, 1.38, 1.48, and 0.36 mug/ml, respectively. Maximal inhibition rates of IL-1beta, IL-6, IL-8, and TNF-alpha production by libanlibanoridin were about 117.5%, 86.22%, 86.41%, and 90.74%, respectively. libanoridin inhibits the mRNA expression of IL-1beta, IL-6, IL-8, and TNF-alpha. libanoridin also inhibits the expression of cyclooxygenase-2.


CONCLUSIONS
These results indicate that libanlibanoridin may be helpful in regulating mast cell-mediated allergic inflammatory response.
Kim
Jae-Joong
JJ
Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Kang
Tae-Hee
TH
Seo
Jae-Uk
JU
Na
Ho-Jeong
HJ
Kim
Su-Jin
SJ
Moon
Phil-Dong
PD
Kim
Na-Hyung
NH
Choi
In-Young
IY
Myung
No-Yil
NY
Hong
Seung-Heon
SH
Kim
Sung-Hoon
SH
Kim
You-Ah
YA
Seo
Young-Wan
YW
Kim
Min-Ho
MH
Park
Jin-Han
JH
Kang
In-Cheol
IC
Um
Jae-Young
JY
Kim
Hyung-Min
HM
Jeong
Hyun-Ja
HJ
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control; D020128:Inhibitory Concentration 50; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D055670:Medicine, Korean Traditional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003374:Coumarins; D016207:Cytokines; D007476:Ionophores; C544330:libanoridin; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate