Effects of thawing, refreezing and storage conditions of tissue samples and protein extracts on 2-DE spot intensity.
We report that reliable quantitative proteome analyses can be performed with tissue samples stored at -80 degrees C for up to 10 years. However, storing protein extracts at 4 degrees C for 24 h and freezing protein extracts at -80 degrees C and thawing them significantly altered 41.6 and 17.5% of all spot intensities on 2-DE gels, respectively. Fortunately, these storing effects did not impair the reliability of quantifying 2-DE experiments. Nonetheless, the results show that freezing and storage conditions should be carefully controlled in proteomic experiments.
Weist
Stephanie
S
Department of Radiology and Nuclear Medicine (Radiochemistry), Charité Universitätsmedizin, Campus Benjamin Franklin, Berlin, Germany.
Brunkau
Cindy
C
Wittke
Janosch
J
Eravci
Murat
M
Broedel
Oliver
O
Krause
Eberhard
E
Stephanowitz
Heike
H
Eravci
Selda
S
Baumgartner
Andreas
A
D000818:Animals; D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005615:Freezing; D008297:Male; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013048:Specimen Handling / Q000379:methods*
D009419:Nerve Tissue Proteins