title: NMR characterisation of the minimal interacting regions of centrosomal proteins 4.1R and NuMA1: effect of phosphorylation.
abstract: BACKGROUND
  Some functions of 4.1R in non-erythroid cells are directly related with its distinct sub-cellular localisation during cell cycle phases. During mitosis, 4.1R is implicated in cell cycle progression and spindle pole formation, and co-localizes with NuMA1. However, during interphase 4.1R is located in the nucleus and only partially co-localizes with NuMA1.
  
  
  RESULTS
  We have characterized by NMR the structural features of the C-terminal domain of 4.1R and those of the minimal region (the last 64 residues) involved in the interaction with NuMA1. This subdomain behaves as an intrinsically unfolded protein containing a central region with helical tendency. The specific residues implicated in the interaction with NuMA1 have been mapped by NMR titrations and involve the N-terminal and central helical regions. The segment of NuMA1 that interacts with 4.1R is phosphorylated during mitosis. Interestingly, NMR data indicates that the phosphorylation of NuMA1 interacting peptide provokes a change in the interaction mechanism. In this case, the recognition occurs through the central helical region as well as through the C-terminal region of the subdomain meanwhile the N-terminal region do not interact.
  
  
  CONCLUSIONS
  These changes in the interaction derived from the phosphorylation state of NuMA1 suggest that phosphorylation can act as subtle mechanism of temporal and spatial regulation of the complex 4.1R-NuMA1 and therefore of the processes where both proteins play a role.
authors.affiliation: Departamento de Espectroscopía y Estructura Molecular, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, 28006 Madrid, Spain. mbruix@iqfr.csic.es
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Treviño
authors.forename: Mar
authors.initials: M
authors.lastname: Rodríguez-Rodríguez
authors.forename: Isabel
authors.initials: I
authors.lastname: Correas
authors.forename: Miguel
authors.initials: M
authors.lastname: Marcilla
authors.forename: Juan P
authors.initials: JP
authors.lastname: Albar
authors.forename: Manuel
authors.initials: M
authors.lastname: Rico
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Jiménez
authors.forename: Marta
authors.initials: M
authors.lastname: Bruix
mesh_terms: D000595:Amino Acid Sequence; D034961:Antigens, Nuclear / Q000737:chemistry*; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D034521:Nuclear Matrix-Associated Proteins / Q000737:chemistry*; D010766:Phosphorylation; D054730:Protein Interaction Domains and Motifs
chemical_list: D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C050045:NUMA1 protein, human; D034521:Nuclear Matrix-Associated Proteins; C028841:erythrocyte membrane band 4.1 protein