Establishment and characterization of a fibroblast cell line derived from the dorsal fin of red sea bream, Pagrus major (Temminck & Schlegel).
The establishment and partial characterization of a continuous cell line from the dorsal fin of red sea bream, Pagrus major, are described. The cell line, designated RSBF-2, has been subcultured for more than 100 passages since its initiation in November 2000. It was optimally maintained at 28 degrees C in Leibovitz L-15 medium with 10% foetal bovine serum. Propagation of RSBF-2 cells was serum dependent and exhibited low plating efficiency (<1.7%). Aside from long-term cryopreservation, the cells could also be kept at 4 degrees C for 72 days. The distribution of the chromosome number was 38-98 with a mode of 48. The RSBF-2 cell line was susceptible to red sea bream iridovirus but only produced a few rounded and refractory cells. Virus-inoculated RSBF-2 cells were then subcultured to generate a persistently infected cell line. RSBF-2 was also very sensitive to the extracellular products of Photobacterium damselae ssp. piscicida and produced significant fluorescent signals after transfection with pEGFP-C3. Analysis of mitochondrial cytochrome b gene sequences revealed 99% identity between the cell line and Pagrus major.
Ku
C-C
CC
Department of Aquaculture, National Penghu University, Penghu, Taiwan. ccku@npu.edu.tw
Lu
C-H
CH
Wang
C-S
CS
D000818:Animals; D001426:Bacterial Proteins / Q000633:toxicity; D002460:Cell Line; D015925:Cryopreservation; D045303:Cytochromes b / Q000235:genetics; D004266:DNA Virus Infections / Q000662:veterinary / Q000821:virology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000821:virology; D005393:Fish Diseases / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics; D017994:Iridovirus / Q000254:growth & development; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000502:physiology; D010776:Photobacterium / Q000378:metabolism; D012087:Reoviridae / Q000502:physiology; D012088:Reoviridae Infections / Q000662:veterinary; D021541:Sea Bream
D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins; D045303:Cytochromes b