title: Molecular analysis of vector genome structures after liver transduction by conventional and self-complementary adeno-associated viral serotype vectors in murine and nonhuman primate models.
abstract: Vectors based on several new adeno-associated viral (AAV) serotypes demonstrated strong hepatocyte tropism and transduction efficiency in both small- and large-animal models for liver-directed gene transfer. Efficiency of liver transduction by AAV vectors can be further improved in both murine and nonhuman primate (NHP) animals when the vector genomes are packaged in a self-complementary (sc) format. In an attempt to understand potential molecular mechanism(s) responsible for enhanced transduction efficiency of the sc vector in liver, we performed extensive molecular studies of genome structures of conventional single-stranded (ss) and sc AAV vectors from liver after AAV gene transfer in both mice and NHPs. These included treatment with exonucleases with specific substrate preferences, single-cutter restriction enzyme digestion and polarity-specific hybridization-based vector genome mapping, and bacteriophage phi29 DNA polymerase-mediated and double-stranded circular template-specific rescue of persisted circular genomes. In mouse liver, vector genomes of both genome formats seemed to persist primarily as episomal circular forms, but sc vectors converted into circular forms more rapidly and efficiently. However, the overall differences in vector genome abundance and structure in the liver between ss and sc vectors could not account for the remarkable differences in transduction. Molecular structures of persistent genomes of both ss and sc vectors were significantly more heterogeneous in macaque liver, with noticeable structural rearrangements that warrant further characterizations.
authors.affiliation: Gene Therapy Program, Department of Pathology, Laboratory of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.forename: You
authors.initials: Y
authors.lastname: Lu
authors.forename: Lawrence T
authors.initials: LT
authors.lastname: Bish
authors.forename: Roberto
authors.initials: R
authors.lastname: Calcedo
authors.forename: James M
authors.initials: JM
authors.lastname: Wilson
authors.forename: Guangping
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D004277:DNA, Single-Stranded / Q000235:genetics; D005822:Genetic Vectors; D016678:Genome; D022781:Hepatocytes / Q000821:virology; D008099:Liver / Q000821:virology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D057079:Pongo; D011323:Primates / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded