MoRic8 Is a novel component of G-protein signaling during plant infection by the rice blast fungus Magnaporthe oryzae.
An insertional mutagenesis screen was used to investigate the biology of plant infection by the devastating rice blast pathogen, Magnaporthe oryzae. Here, we report the identification of a new mutant, LY-130, which is defective in multiple steps during infection-related morphogenesis and pathogenicity. Analysis of the mutation confirmed an insertion into gene MoRIC8, which encodes a 480-amino-acid protein that is a putative homologue of the Ric8 regulator of GTP-binding protein (G-protein) signaling, previously described in animals. Targeted gene deletion mutants of MoRIC8 were nonpathogenic and impaired in cellular differentiation associated with sporulation, sexual development, and plant infection. MoRic8 physically interacts with the Galpha subunit MagB in yeast two-hybrid assays and appears to act upstream of the cyclic AMP response pathway that is necessary for appressorium morphogenesis. Taken together, our results indicate that MoRic8 may act as a novel regulator of the G-protein signaling during infection-related development of rice blast fungus M. oryzae.
Li
Ya
Y
State Key Laboratory For Rice Biology, Biotechnology Institute, Zhejian University, Huajiachi Campus, Hangzhou, China.
Yan
Xia
X
Wang
Hong
H
Liang
Shen
S
Ma
Wei-Bin
WB
Fang
Min-Yan
MY
Talbot
Nicholas J
NJ
Wang
Zheng-Yi
ZY
D000595:Amino Acid Sequence; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000382:microbiology; D020082:Magnaporthe / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D014774:Virulence / Q000235:genetics
D005656:Fungal Proteins; C549596:MoRic8 protein, Magnaporthe oryzae; D049452:Green Fluorescent Proteins; D000242:Cyclic AMP; D019204:GTP-Binding Proteins