title: Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV).
abstract: The new H1N1 swine-origin influenza virus (S-OIV) strain is a global health problem. The elucidation of the virus-host relationship is crucial for the control of the new infection. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza strain at very low concentrations were cloned before the emergence of S-OIV from a patient who had a broad-range H1N1 serum neutralizing activity. The two HMabs neutralized all tested H1N1 strains, including S-OIV and a swine strain with IC(50) ranging from 2 to 7 microg/ml. Data demonstrate that infection with previously circulating H1N1 strains can elicit antibodies neutralizing S-OIV. Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection.
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy. Electronic address: Burioni.roberto@hsr.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Burioni
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Canducci
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Nicasio
authors.initials: N
authors.lastname: Mancini
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Clementi
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Sassi
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Donata
authors.initials: D
authors.lastname: De Marco
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Roberta Antonia
authors.initials: RA
authors.lastname: Diotti
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Saita
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Sampaolo
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sautto
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Pianezze
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, via Olgettina 60, 20132 Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002645:Chickens / Q000821:virology; D004306:Dose-Response Relationship, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000276:immunology / Q000821:virology; D008875:Middle Aged; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000821:virology; D016415:Sequence Alignment; D013552:Swine / Q000821:virology; D010948:Viral Plaque Assay
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments