[Effects of intraorbital injection of 6.0% carboxymethylcellulose in rabbits: histological and ocular mechanics evaluation].
OBJECTIVE
To determine the effects of 6.0% carboxymethylcellulose (CMC) in modifying the oculomotor system of rabbits by means of viscoelastic action forces, the durability of this effect and possible inflammatory reaction.


METHODS
Twenty-five New Zealand rabbits were divided into two experimental groups: one treated with a peribulbar injection of 6.0% CMC and a control group submitted to peribulbar injection of 3.0 cc of physiological saline. The group submitted to peribulbar CMC injection was divided into four subgroups respectively receiving the following volumes: 1.0, 1.5, 2.0 and 3.0 cc. The force needed to promote tangential adduction dislocations, external ophthalmologic signs and intraocular pressure were evaluated. The animals were sacrificed on the 60th day after the injection for histological analysis.


RESULTS
The mean force detected 60 days after the injection of 6.0% CMC was lower in the 1.0 treated subgroup and higher in the 1.5, 2.0 and 3.0 treated subgroups relative to the force before injection. Histological analysis revealed histiocytic infiltration with fibrosis in the subgroups in which there was an increase in force and the presence of CMC in periocular tissues.


CONCLUSIONS
6.0% CMC acted as a modifier of the oculomotor system of rabbits, facilitating or impairing movements. It was not possible to conclude whether the increase in force occurred as a consequence of the inflammatory process alone or of the sum of inflammation and a possible viscous attrition provoked by CMC.
Simão
Maria Lúcia Habib
ML
Departamento de Patologia e Medicina Legal, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brasil, SP. malu.habibsimao@gmail.com
Chahud
Fernando
F
Bicas
Harley Edison Amaral
HE
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage* / Q000009:adverse effects; D054459:Elasticity Imaging Techniques / Q000379:methods; D005260:Female; D006644:Histiocytes / Q000473:pathology; D023421:Models, Animal; D009801:Oculomotor Muscles / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D009915:Orbit / Q000187:drug effects*; D055622:Orbital Myositis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits
D002266:Carboxymethylcellulose Sodium