Ultrafast LC method for purification and metabolite analysis of PET probes.
An ultrafast and efficient high-performance liquid chromatographic (LC) method was developed to purify positron emission tomography (PET) radiopharmaceuticals as well as for metabolite analysis of the plasma sample. Chromatographic separation was achieved on a short (60 mm length) semipreparative (10 mm I.D.) column packed with 2.5-mum particles using a mixture of acetonitrile and sodium phosphate buffer as the mobile phase at a flow rate of 8-10 ml/min. Under the optimum conditions, excellent separation of the target PET probe was obtained from chemical/radiochemical impurities or radioactive metabolites with a very short run time of 2 min. This characteristic enabled significant shortening of the purification and evaporation processes in the production of short-lived radiopharmaceuticals and highly sensitive radiometric analysis with good temporal resolution during the metabolism study.
Nakao
Ryuji
R
Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan. nakaor@nirs.go.jp
Ito
Takehito
T
Hayashi
Kazutaka
K
Fukumura
Toshimitsu
T
Suzuki
Kazutoshi
K
D002250:Carbon Radioisotopes / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006801:Humans; D049268:Positron-Emission Tomography; D011687:Purines / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D019275:Radiopharmaceuticals / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D013997:Time Factors
D002250:Carbon Radioisotopes; C502525:N-benzyl-N-ethyl-2-(7,8-dihydro-7-methyl-8-oxo-2-phenyl-9H-purin-9-yl)acetamide; D011687:Purines; D019275:Radiopharmaceuticals