Modification to the renneting functionality of casein micelles caused by nonionic surfactants.
Nonionic emulsifiers of small molecular weight such as polysorbates are widely used in dairy products. Nevertheless, the mechanism of interaction between these surfactants and milk proteins is not yet fully understood. This work investigated the effect of Tween 20 on casein micelles by studying the renneting behavior of skim milk in the presence of different amounts of surfactant. The presence of Tween accelerated both the first and second phase of renneting in skim milk. The gel obtained showed a higher elastic modulus than that of a skim milk gel, but also showed similar brittleness. By varying the size of the surfactant (Tween 20 or Tween 80) as well as the colloidal state of the proteins in solution, it was possible to demonstrate that the surfactant did not have a direct effect on the activity of the enzyme, but rather had a direct effect on the casein micelles. The effect of surfactant on the gelation point was reduced by increasing surfactant size. The presence of Tween caused an increase in the size of the micelles without affecting their stability. In addition, Tween did not alter the amount of caseins free in the serum phase. These findings can contribute to improving our ability to custom design final structures in rennet-induced gels, though further studies are needed to fully understand the mechanism at play when casein micelles are enzymatically cleaved in the presence of nonionic surfactants of small molecular weight.
Ion Titapiccolo
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Department of Food Science, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Corredig
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Alexander
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D000818:Animals; D002364:Caseins / Q000737:chemistry*; D012085:Chymosin / Q000737:chemistry; D005511:Food Handling; D008823:Micelles; D008892:Milk / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
D002364:Caseins; D008823:Micelles; D011136:Polysorbates; D013501:Surface-Active Agents; C025350:rennet; D012085:Chymosin