Nocardiopsis nikkonensis sp. nov., isolated from a compost sample.
An actinomycete strain, designated YU1183-22(T), was isolated from a compost sample collected in Nikko, Japan. The isolate formed white aerial mycelium with relatively long aerial hyphae showing chains of arthrospores. Strain YU1183-22(T) grew with 0-10 % (w/v) NaCl, at pH 6-11 and at 10-37 °C (optimum 30 °C). Strain YU1183-22(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H(10)) and MK-10(H(8)). The polar lipids were phosphatidylcholine and phosphatidylglycerol. The major cellular fatty acids were iso-C(16 : 0), anteiso-C(17 : 0) and tuberculostearic acid. The G+C content of the DNA was 72.3 mol%. Chemotaxonomic and morphological characterization clearly demonstrated that strain YU1183-22(T) belonged to the genus Nocardiopsis. Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to Nocardiopsis salina YIM 90010(T) (98.0 % 16S rRNA gene sequence similarity), Nocardiopsis xinjiangensis YIM 90004(T) (97.9 %) and Nocardiopsis kunsanensis HA-9(T) (97.3 %). However, DNA-DNA relatedness as well as physiological and biochemical analyses showed that strain YU1183-22(T) could be differentiated from its closest phylogenetic relatives. It is proposed that this strain be classified as a representative of a novel species of the genus Nocardiopsis, with the name Nocardiopsis nikkonensis sp. nov. The type strain is YU1183-22(T) (=NBRC 102170(T) =KCTC 19666(T)).
Yamamura
Hideki
H
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
Ohkubo
Shin-Ya
SY
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
Ishida
Yuumi
Y
NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Otoguro
Misa
M
NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Tamura
Tomohiko
T
NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Hayakawa
Masayuki
M
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid