title: Single nucleotide polymorphism in the methyl-CpG binding domain 4 gene and the risk for immune thrombocytopenic purpura in Chinese population.
abstract: Epigenetics might contribute to autoimmune diseases including immune thrombocytopenic purpura (ITP). Methyl-CpG binding domain protein 4 (MBD4) plays an important role in DNA methylation and transcriptional regulation of gene expression. The polymorphism of the MBD4 gene may influence MBD4 activity on gene expression profiles, thereby influencing individual susceptibility to ITP. To verify this hypothesis, we investigated the association between the MBD4 polymorphism and the risk for ITP in the Chinese population. The polymorphism of MBD4 rs140693 was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In this study, there was no significant difference in genotype and alleles distribution between the ITP patients and the controls. Similar results were observed between the two groups when stratified by age and disease course including acute childhood, chronic childhood, acute adult and chronic adult. In the conclusion, MBD4 polymorphism may not be a stratification marker to predict the susceptibility to ITP, at least in Chinese population.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
authors.forename: Weiting
authors.initials: W
authors.lastname: Du
authors.forename: Donghai
authors.initials: D
authors.lastname: Wang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.forename: Tao
authors.initials: T
authors.lastname: Sui
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004706:Endodeoxyribonucleases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000503:physiopathology; D012307:Risk Factors; D055815:Young Adult
chemical_list: D004706:Endodeoxyribonucleases; C118712:MBD4 protein, human