Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum.
A simplest method was followed for isolation of high quality genomic DNA form thallus of Plagiochasma appendiculatum that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4M) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis.
Soni
Arvind
A
Plant Genomic Lab, Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Luckonw 266 001, India.
Kumar
Anil
A
D018744:DNA, Plant / Q000235:genetics*; D035926:Hepatophyta / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant