title: 2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC/UV detection of amino acids.
abstract: The use of 2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC analysis of amino acids is proposed. The compound reacts under mild conditions (10min at ambient temperature) with primary amino groups. The derivatization conditions to obtain quantitative reaction were optimised by considering different parameters (temperature, pH and reagent concentration) using l-Val as the model compound. The synthesized l-Val derivative was characterized by (1)H NMR and UV. The derivatives of 19 amino acids were separated by reversed-phase HPLC and detected at lambda=320nm. The method was applied successfully to the qualitative and quantitative analysis of commercial polyamino acid preparations.
authors.affiliation: Department of Pharmaceutical Science, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. rita.gatti2@unibo.it <rita.gatti2@unibo.it>
authors.forename: Rita
authors.initials: R
authors.lastname: Gatti
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Gioia
authors.forename: Alberto
authors.initials: A
authors.lastname: Leoni
authors.forename: Aldo
authors.initials: A
authors.lastname: Andreani
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: C552731:2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde; D000447:Aldehydes; D000596:Amino Acids; D007202:Indicators and Reagents; D011758:Pyrroles