Extractions of isoquinoline alkaloids with butanol and octanol.
Six different isoquinoline alkaloids (sanguinarine, chelerythrine, berberine, coptisine, allocryptopine, and protopine) were extracted by butanol and octanol from aqueous solution, pH 4.5. The samples were analyzed by HPLC. Butanol extraction was non-selective, alkaloids passed into organic phase in 83-98%. Octanol extraction provided more selective yields: sanguinarine 99%, chelerythrine 94%, berberine 18%, coptisine 16%, allocryptopine 7.5%, protopine 7%. Further, we tested octanol treatment of extract from Dicranostigma lactucoides. The octanol extraction yields were also selective: sanguinarine 98%, chelerythrine 92%, chelirubine 92.5%, protopine 6% and allocryptopine 3.5%. 6-Butoxy-5,6-dihydrosanguinarine and 6-butoxy-5,6-dihydrochelerythrine were prepared and their NMR and MS data are reported and discussed.
Gregorová
Jana
J
Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno, Czech Republic. jgregorova@med.muni.cz
Babica
Jan
J
Marek
Radek
R
Paulová
Hana
H
Táborská
Eva
E
Dostál
Jirí
J
D053119:Benzophenanthridines / Q000302:isolation & purification*; D000440:Butanols / Q000737:chemistry; D007546:Isoquinolines / Q000302:isolation & purification*; D000442:Octanols / Q000737:chemistry
D053119:Benzophenanthridines; D000440:Butanols; D007546:Isoquinolines; D000442:Octanols