title: Effect of BMP-9 and its derived peptide on the differentiation of human white preadipocytes.
abstract: Several studies have shown that bone morphogenetic proteins (BMPs) can influence adipogenic and osteogenic cell lineages. We have shown that a peptide derived from BMP-9 (pBMP-9) at 400 ng/ml inhibits the proliferation of preosteoblasts and induces differentiation. We have now determined the effects of pBMP-9 (400 ng/ml) and equimolar concentrations of BMP-2 (100 ng/ml), BMP-9 (84.6 ng/ml) and pBMP-9 (9.04 ng/ml) on human white preadipocytes (HWP). pBMP-9 dose dependently reduced the proliferation of HWP without affecting the number of apoptotic cells. Incubation of the cells for 1 h with BMP-2, BMP-9 or pBMP-9 activated the Smad1/5/8 pathway, while incubation for 7 days in adipocyte differentiation (AD) serum-free medium containing ciglitazone and equimolar concentrations of BMP-2, BMP-9 or pBMP-9 enhanced the levels of mRNA of the adipogenic markers aP2 and adipoQ and increased the number of lipid vesicles. Thus, pBMP-9, like BMP-9, can increase the AD of HWP in AD serum-free medium.
authors.affiliation: Laboratory of Cell-Biomaterial Biohybrid Systems, Department of Chemical Engineering and Biotechnological Engineering, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, Québec, J1K 2R1, Canada. etienne.lord@usherbrooke.ca
authors.forename: E
authors.initials: E
authors.lastname: Lord
authors.forename: E
authors.initials: E
authors.lastname: Bergeron
authors.forename: H
authors.initials: H
authors.lastname: Senta
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.forename: N
authors.initials: N
authors.lastname: Faucheux
mesh_terms: D052438:Adipocytes, White / Q000187:drug effects* / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects*; D000328:Adult; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005819:Genetic Markers; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D047495:PPAR gamma / Q000032:analysis; D010455:Peptides / Q000494:pharmacology; D051785:Smad Proteins / Q000187:drug effects; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; C525887:GDF2 protein, human; D005819:Genetic Markers; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors; D047495:PPAR gamma; D010455:Peptides; D051785:Smad Proteins; D045162:Thiazolidinediones; C039671:ciglitazone