title: Intrageneric relationships of members of the genus Rhodopseudomonas.
abstract: The intrageneric structure of the genus Rhodopseudomonas was evaluated by studying sequence information on 16S rRNA genes, 16S-23S rRNA gene internal transcribed spacer (ITS) regions, and puf genes using 33 test strains. The topology of phylogenetic trees based on these sequences was similar to those of every other independent method for tree construction. These phylogenetic data indicated that the test strains were grouped into at least 7 clusters possibly at the species level. This was supported by genomic DNA-DNA similarities among 12 representative test strains selected from these clusters. Our molecular data confirmed that the currently available strains of Rhodopseudomonas (Rps.) palustris are genetically quite heterogeneous within the genus. For example, Rps. palustris strains DSM 123(T) and ATCC 17001(T) are different from each other at the species level despite their status as the type strain of the species. Rps. palustris strain ATCC 17005 and the full genome-sequenced strains BisA53, BisB18, BisB5, and HaA2 should be re-classified into different species from Rps. palustris or as novel species of the genus Rhodopseudomonas.
authors.affiliation: Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Okamura
authors.forename: Koji
authors.initials: K
authors.lastname: Takata
authors.forename: Akira
authors.initials: A
authors.lastname: Hiraishi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D045342:Light-Harvesting Protein Complexes / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012241:Rhodopseudomonas / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; C096433:PufL protein, Bacteria; C098543:PufM protein, Bacteria; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S