title: Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).
abstract: Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Eustermann
authors.forename: Christoph
authors.initials: C
authors.lastname: Brockmann
authors.forename: Pawan Vinod
authors.initials: PV
authors.lastname: Mehrotra
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Yang
authors.forename: David
authors.initials: D
authors.lastname: Loakes
authors.forename: Stephen C
authors.initials: SC
authors.lastname: West
authors.forename: Ivan
authors.initials: I
authors.lastname: Ahel
authors.forename: David
authors.initials: D
authors.lastname: Neuhaus
mesh_terms: D000595:Amino Acid Sequence; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016335:Zinc Fingers
chemical_list: D010750:Phosphoproteins; D000075223:Poly-ADP-Ribose Binding Proteins; D011064:Poly Adenosine Diphosphate Ribose; C520557:APLF protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase