Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer.
A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Deltalambda=10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 x 10(-9) to 4.50 x 10(-6) mol L(-1), 3.64 x 10(-8) to 2.20 x 10(-4) mol L(-1), 8.18 x 10(-9) to 1.20 x 10(-4) mol L(-1), 3.26 x 10(-9) to 8.50 x 10(-5) mol L(-1) and 4.88 x 10(-9) to 5.50 x 10(-6) mol L(-1), respectively. The limits of detection (LOD) were found to be 5.25 x 10(-10) mol L(-1) for 1-OHP, 1.10 x 10(-8) mol L(-1) for 1-NAP, 2.46 x 10(-9) mol L(-1) for 2-NAP, 9.77 x 10(-10) mol L(-1) for 9-OHPe and 1.46 x 10(-9) mol L(-1) for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method.
Kang
Rong-Hui
RH
College of Public Health, University of South China, Hengyang 421001, PR China.
Wang
Yong-Sheng
YS
Yang
Hong-Mei
HM
Li
Gui-Rong
GR
Tan
Xuan
X
Xue
Jin-Hua
JH
Zhang
Jin-Quan
JQ
Yuan
Yu-Kun
YK
Shi
Lin-Fei
LF
Xiao
Xi-Lin
XL
D005449:Fluorenes / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009284:Naphthols / Q000652:urine; D010616:Phenanthrenes / Q000652:urine; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000652:urine*; D011136:Polysorbates / Q000737:chemistry*; D011721:Pyrenes / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry*
D005449:Fluorenes; D009284:Naphthols; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011136:Polysorbates; D011721:Pyrenes; D013501:Surface-Active Agents; C029350:1-naphthol; C091375:9-phenanthrol; C477779:2-hydroxyfluorene; C033146:1-hydroxypyrene; C028405:2-naphthol