Violacein-producing Collimonas sp. from the sea surface microlayer of costal waters in Trøndelag, Norway.
A new strain belonging to the genus Collimonas was isolated from the sea surface microlayer off the coast of Trøndelag, Norway. The bacterium, designated Collimonas CT, produced an antibacterial compound active against Micrococcus luteus. Subsequent studies using LC-MS identified this antibacterial compound as violacein, known to be produced by several marine-derived bacteria. Fragments of the violacein biosynthesis genes vioA and vioB were amplified by PCR from the Collimonas CT genome and sequenced. Phylogenetic analysis of these sequences demonstrated close relatedness of the Collimonas CT violacein biosynthetic gene cluster to those in Janthinobacterium lividum and Duganella sp., suggesting relatively recent horizontal gene transfer. Considering diverse biological activities of violacein, Collimonas CT shall be further studied as a potential producer of this compound.
Hakvåg
Sigrid
S
Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway. sigrid.hakvag@biotech.ntnu.no
Fjaervik
Espen
E
Klinkenberg
Geir
G
Borgos
Sven Even F
SE
Josefsen
Kjell D
KD
Ellingsen
Trond E
TE
Zotchev
Sergey B
SB
D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects; D016984:Enterococcus faecium / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005798:Genes, Bacterial; D007211:Indoles / Q000302:isolation & purification* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008835:Micrococcaceae / Q000187:drug effects; D008969:Molecular Sequence Data; D005810:Multigene Family; D009664:Norway; D042802:Oxalobacteraceae / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny
D000890:Anti-Infective Agents; D007211:Indoles; C063155:violacein