4-Chlorobenzoyl berbamine induces apoptosis and G2/M cell cycle arrest through the PI3K/Akt and NF-kappaB signal pathway in lymphoma cells.
Berbamine is an herbal compound derived from Berberis amurensis, which is used in Chinese traditional medicine. However, few studies have investigated this anti-tumor effect or the underlying mechanisms of berbamine on lymphoma cells. We investigate the effect, as well as the mechanism of action, of 4-chlorobenzoyl berbamine (BBD9) on Raji, L428, Namalwa and Jurkat lymphoma cells lines. Our findings show that BBD9 inhibits cell proliferation and induces cell apoptosis in lymphoma cell lines as well as G2/M cell cycle arrest through PI3K/Akt and NF-kappaB signaling pathways in a caspase-dependent manner. These results may provide new insights into the treatment of lymphoma.
Du
Hua-Ping
HP
Institute of Hematology, The First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang 310003, P.R. China.
Shen
Jia-Kun
JK
Yang
Min
M
Wang
Yun-Qui
YQ
Yuan
Xiang-Qui
XQ
Ma
Qiu-Ling
QL
Jin
Jie
J
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D056744:Cyclin B1 / Q000502:physiology; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology; D016328:NF-kappa B / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000502:physiology
C548958:4-chlorobenzoylberbamine; D000970:Antineoplastic Agents; D044182:Benzylisoquinolines; C534699:CCNB1 protein, human; D056744:Cyclin B1; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human