title: Autoantibodies against IL-17A, IL-17F, and IL-22 in patients with chronic mucocutaneous candidiasis and autoimmune polyendocrine syndrome type I.
abstract: Most patients with autoimmune polyendocrine syndrome type I (APS-I) display chronic mucocutaneous candidiasis (CMC). We hypothesized that this CMC might result from autoimmunity to interleukin (IL)-17 cytokines. We found high titers of autoantibodies (auto-Abs) against IL-17A, IL-17F, and/or IL-22 in the sera of all 33 patients tested, as detected by multiplex particle-based flow cytometry. The auto-Abs against IL-17A, IL-17F, and IL-22 were specific in the five patients tested, as shown by Western blotting. The auto-Abs against IL-17A were neutralizing in the only patient tested, as shown by bioassays of IL-17A activity. None of the 37 healthy controls and none of the 103 patients with other autoimmune disorders tested had such auto-Abs. None of the patients with APS-I had auto-Abs against cytokines previously shown to cause other well-defined clinical syndromes in other patients (IL-6, interferon [IFN]-gamma, or granulocyte/macrophage colony-stimulating factor) or against other cytokines (IL-1beta, IL-10, IL-12, IL-18, IL-21, IL-23, IL-26, IFN-beta, tumor necrosis factor [alpha], or transforming growth factor beta). These findings suggest that auto-Abs against IL-17A, IL-17F, and IL-22 may cause CMC in patients with APS-I.
authors.affiliation: Laboratory of Human Genetics of Infectious Diseases, Necker Branch, Institut National de la Santé et de la Recherche Médicale (INSERM), U550, 75015 Paris, France. anne.puel@inserm.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Puel
authors.forename: Rainer
authors.initials: R
authors.lastname: Döffinger
authors.forename: Angels
authors.initials: A
authors.lastname: Natividad
authors.forename: Maya
authors.initials: M
authors.lastname: Chrabieh
authors.forename: Gabriela
authors.initials: G
authors.lastname: Barcenas-Morales
authors.forename: Capucine
authors.initials: C
authors.lastname: Picard
authors.forename: Aurélie
authors.initials: A
authors.lastname: Cobat
authors.forename: Marie
authors.initials: M
authors.lastname: Ouachée-Chardin
authors.forename: Antoine
authors.initials: A
authors.lastname: Toulon
authors.forename: Jacinta
authors.initials: J
authors.lastname: Bustamante
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Muhsen
authors.forename: Mohammed
authors.initials: M
authors.lastname: Al-Owain
authors.forename: Peter D
authors.initials: PD
authors.lastname: Arkwright
authors.forename: Colm
authors.initials: C
authors.lastname: Costigan
authors.forename: Vivienne
authors.initials: V
authors.lastname: McConnell
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Cant
authors.forename: Mario
authors.initials: M
authors.lastname: Abinun
authors.forename: Michel
authors.initials: M
authors.lastname: Polak
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Bougnères
authors.forename: Dinakantha
authors.initials: D
authors.lastname: Kumararatne
authors.forename: László
authors.initials: L
authors.lastname: Marodi
authors.forename: Amit
authors.initials: A
authors.lastname: Nahum
authors.forename: Chaim
authors.initials: C
authors.lastname: Roifman
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blanche
authors.forename: Alain
authors.initials: A
authors.lastname: Fischer
authors.forename: Christine
authors.initials: C
authors.lastname: Bodemer
authors.forename: Laurent
authors.initials: L
authors.lastname: Abel
authors.forename: Desa
authors.initials: D
authors.lastname: Lilic
authors.forename: Jean-Laurent
authors.initials: JL
authors.lastname: Casanova
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D015551:Autoimmunity; D015153:Blotting, Western; D002178:Candidiasis, Chronic Mucocutaneous / Q000097:blood / Q000209:etiology / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology*; D007378:Interleukins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016884:Polyendocrinopathies, Autoimmune / Q000097:blood / Q000150:complications / Q000276:immunology*; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D001323:Autoantibodies; D007371:Interferon-gamma; D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22