title: Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma.
abstract: In the cytosol of lipogenic tissue, ketone bodies are activated by AACS (acetoacetyl-CoA synthetase) and incorporated into cholesterol and fatty acids. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. In order to elucidate the mechanism controlling the gene expression of human AACS and to clarify its physiological role, we isolated the human promoter, characterized the elements required to initiate transcription and analysed the expression of the gene in response to PPARgamma (peroxisome-proliferator-activated receptor gamma), an inducer of adipogenesis. We show that the human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1).
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Biomedicine of Barcelona University, Spain.
authors.forename: Francesca
authors.initials: F
authors.lastname: Aguiló
authors.forename: Nuria
authors.initials: N
authors.lastname: Camarero
authors.forename: Joana
authors.initials: J
authors.lastname: Relat
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Marrero
authors.forename: Diego
authors.initials: D
authors.lastname: Haro
mesh_terms: D050156:Adipogenesis; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D047495:PPAR gamma / Q000502:physiology*; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D047495:PPAR gamma; D016329:Sp1 Transcription Factor; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase