title: Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen.
abstract: Newly designed primers for [Fe-Fe]-hydrogenases indicated that (i) fermenters, acetogens, and undefined species in a fen harbor hitherto unknown hydrogenases and (ii) Clostridium- and Thermosinus-related primary fermenters, as well as secondary fermenters related to sulfate or iron reducers might be responsible for hydrogen production in the fen. Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and inconsistencies between 16S rRNA gene- and [Fe-Fe]-hydrogenase-based phylogenies, necessitating appropriate qualification of [Fe-Fe]-hydrogenase gene data for diversity analyses.
authors.affiliation: Department of Ecological Microbiology, University of Bayreuth, 95445 Bayreuth, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schmidt
authors.forename: Harold L
authors.initials: HL
authors.lastname: Drake
authors.forename: Marcus A
authors.initials: MA
authors.lastname: Horn
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006864:Hydrogenase / Q000235:genetics*; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D056186:Metagenomics; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007506:Iron-Sulfur Proteins; D012336:RNA, Ribosomal, 16S; C118830:iron hydrogenase; D006864:Hydrogenase