The use of quantum dot nanocrystals in multicolor flow cytometry.
Because of their unique fluorescence properties, quantum dots (QDs) represent a promising new technology in the realm of multicolor flow cytometry. Although commercial reagents and applications for the technology are still in the early phases of their development, the strategies and considerations necessary for successful use are becoming known. This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology.
Chattopadhyay
Pratip K
PK
ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA. pchattop@mail.nih.gov
Perfetto
Stephen P
SP
Yu
Joanne
J
Roederer
Mario
M
D000818:Animals; D003116:Color; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D045663:Quantum Dots