Simultaneous analysis of endogenetic and ectogenic plant hormones by pressurized capillary electrochromatography.
Pressurized CEC (pCEC) with UV detection was developed for the simultaneous analysis of endogenetic and ectogenic plant hormones. Effects of some crucial factors including mobile phase, buffer concentration, pH value and applied voltage on pCEC separation were investigated, respectively. Under the optimum conditions, namely a packed capillary column (3 microm Hypersil C(18), 45 cm total length with 100 microm id x 20 cm effective length) as the separation channel; ACN: 10 mmol/L phosphate (50:50, v/v, %) as the mobile phase, pH 4.0; pump flow rate 0.035 mL/min; separation voltage -10 kV; UV wavelength 222 nm, a complete separation of five hormones was accomplished. The pCEC assay presented good linearity (0.8-100 microg/mL), with LOD of 0.2 microg/mL and R in range of 0.998-0.999. Meanwhile, a lack-of-fit test was performed to evaluate these linear models. Results showed that no significant lack-of-fit was observed and these models were appropriate (alpha=0.05).
Lu
Qiaomei
Q
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Province Key Laboratory of Analysis and Detection for Food Safety, and Department of Chemistry, Fuzhou, Fujian, P. R. China.
Zhang
Lan
L
Chen
Lihui
L
Lu
Minghua
M
Tong
Ping
P
Chen
Guonan
G
D053801:Capillary Electrochromatography; D015394:Molecular Structure; D010937:Plant Growth Regulators / Q000737:chemistry* / Q000145:classification*; D015203:Reproducibility of Results; D013997:Time Factors
D010937:Plant Growth Regulators