Single domain antibody templated nanoparticle resistors for sensing.
The diversity of biomolecules enables materials to be synthesized at ambient conditions into complex shapes and structures while affording improved control over nanoparticle synthesis, structures, properties, and functionality. Also, biomolecules can impart recognition and sensing capabilities to metal nanoparticles. Here, llama single domain antibodies selected towards the beta-subunit of cholera toxin were used to template the synthesis of gold nanoparticles and subsequently assembled onto electrodes via dielectrophoresis. These assemblies were then tested for the detection of cholera toxin via changes in the conductance of antibody coated gold nanoparticles.
Slocik
Joseph M
JM
Nanostructured & Biological Materials Branch, Materials & Manufacturing Directorate, Air Force Research Lab, Wright-Patterson Air Force Base, OH 45433, USA.
Kim
Sang Nyon
SN
Auvil
Tyler
T
Goldman
Ellen R
ER
Liu
Jinny
J
Naik
Rajesh R
RR
D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002772:Cholera Toxin / Q000032:analysis* / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D017097:Electric Impedance; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D017434:Protein Structure, Tertiary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D002772:Cholera Toxin