title: Combined mucosal and systemic immunity following pulmonary delivery of ISCOMATRIX adjuvanted recombinant antigens.
abstract: Deep pulmonary delivery of an influenza ISCOMATRIX vaccine has previously been shown to induce a combined mucosal and systemic antibody response. To explore whether this combined response is influenced by intrinsic properties of the component antigen, we examined the efficacy of deep pulmonary delivery of ISCOMATRIX vaccines containing different recombinant antigens, specifically gB glycoprotein from cytomegalovirus and a fragment of catalase from Helicobacter pylori. Both these vaccines induced antigen-specific mucosal and systemic immunity, as well as antigen-specific proliferative cellular responses. Pulmonary immunisation with ISCOMATRIX vaccines may therefore be a generic way of inducing combined systemic and mucosal immunity.
authors.affiliation: Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Melbourne, VIC 3010, Australia.
authors.forename: Ana
authors.initials: A
authors.lastname: Vujanic
authors.forename: Janet L K
authors.initials: JL
authors.lastname: Wee
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Snibson
authors.forename: Stirling
authors.initials: S
authors.lastname: Edwards
authors.forename: Martin
authors.initials: M
authors.lastname: Pearse
authors.forename: Charles
authors.initials: C
authors.lastname: Quinn
authors.forename: Margaret
authors.initials: M
authors.lastname: Moloney
authors.forename: Shirley
authors.initials: S
authors.lastname: Taylor
authors.forename: Jean-Pierre Y
authors.initials: JP
authors.lastname: Scheerlinck
authors.forename: Philip
authors.initials: P
authors.lastname: Sutton
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000280:Administration, Inhalation; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002374:Catalase / Q000276:immunology; D049109:Cell Proliferation; D002784:Cholesterol / Q000008:administration & dosage*; D004338:Drug Combinations; D005260:Female; D016480:Helicobacter pylori / Q000201:enzymology; D007279:Injections, Subcutaneous; D008214:Lymphocytes / Q000276:immunology; D010743:Phospholipids / Q000008:administration & dosage*; D012503:Saponins / Q000008:administration & dosage*; D012756:Sheep; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D004338:Drug Combinations; C493786:ISCOMATRIX; D010743:Phospholipids; D012503:Saponins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D002784:Cholesterol; D002374:Catalase