Differential effect of chondroitin-4-sulfate on the immediate and delayed prostaglandin E2 release from osteoblasts.
The present study examines the effect of chondroitin-4-sulfate (C4S) on the immediate (non-inflammatory conditions) and the delayed (inflammatory conditions) prostaglandin E(2) (PGE(2)) release from rat calvarial osteoblasts. An immediate low release of PGE(2) was induced by PAF, phorbol ester and arachidonic acid but not by IL1beta, TNF-alpha and LPS whereas a delayed high release of PGE(2) was induced by the inflammatory agents IL1beta, TNF-alpha and LPS but not by PAF, phorbol ester and arachidonic acid. C4S had no effect on the immediate PGE(2) release but inhibited the delayed release of PGE(2). IL1beta, TNF-alpha and LPS enhanced the expression of COX-2 and mPGES1 whereas phorbol ester enhanced COX-2 expression only. PAF and arachidonic acid had no effect on the expression of COX-2 and mPGES1. C4S inhibited the enhanced expression of COX-2 and mPGES1 but had no effect on the IL1beta-induced decrease of I-kappaBalpha and nuclear translocation of NF-kappaB. These results indicate that the beneficial effects of C4S in bone inflammatory diseases might be due to a specific inhibition of the delayed high PGE(2) release from osteoblasts.
Kunze
Reiner
R
Institute of Physiological Chemistry, Carl Gustav Carus Faculty of Medicine, Dresden University of Technology, Fiedlerstrasse 42, D-01307 Dresden, Germany. reiner.kunze@mailbox.tu-dresden.de
Hempel
Ute
U
Dieter
Peter
P
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism*; D007249:Inflammation / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D012886:Skull / Q000166:cytology
D016328:NF-kappa B; D016718:Arachidonic Acid; D002809:Chondroitin Sulfates; D015232:Dinoprostone