title: Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia.
abstract: OBJECTIVE
  To systematically search for leukemic cells in cryopreserved ovarian cortex from Danish female patients with leukemia, who had ovarian cortex cryopreserved for fertility preservation before potentially sterilizing treatment.
  
  
  METHODS
  Retrospective analysis of data in a clinical project.
  
  
  METHODS
  University hospital laboratories.
  
  
  METHODS
  In total, 26 patients diagnosed with leukemia, who had ovarian tissue cryopreserved before potentially sterilizing chemotherapy and conditioning.
  
  
  METHODS
  Ovarian cortex from each patient was examined with histology and immunohistochemistry. In addition, in eight cases a specific chromosomal abnormality could be used as a genetic marker for detection of malignant cells by polymerase chain reaction (PCR).
  
  
  METHODS
  Evidence of malignant cells by immunohistochemistry or PCR.
  
  
  RESULTS
  Histology and immunohistochemistry did not reveal malignant cell infiltration in the ovarian cortex of any of the patients. In six of the eight patients (75%) with chromosomal abnormalities in the malignant cells, PCR showed evidence of leukemic cells in the ovarian tissue.
  
  
  CONCLUSIONS
  Immunohistochemistry was unable to locate leukemic cells in the ovarian cortex; however, PCR detected potentially malignant cells in the majority of cases. The viability and malignancy of these cells remains to be determined. At present, reimplantation of ovarian cortex to leukemia patients cannot be recommended.
authors.affiliation: Laboratory of Reproductive Biology and The Fertility Clinic, Department of Clinical Genetics, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark. mikro@dadlnet.dk
authors.forename: Mikkel
authors.initials: M
authors.lastname: Rosendahl
authors.forename: Morten Tolstrup
authors.initials: MT
authors.lastname: Andersen
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ralfkiær
authors.forename: Lars
authors.initials: L
authors.lastname: Kjeldsen
authors.forename: Mette Klarskov
authors.initials: MK
authors.lastname: Andersen
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000379:methods; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007247:Infertility, Female / Q000517:prevention & control; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D018365:Neoplasm, Residual; D010053:Ovary; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor