title: Evolutionary analysis for the functional divergence of the Spo0A protein: the key sporulation control element.
abstract: Sporulation involves inducing scores of genes in a temporally and spatially coordinated cell development programme. Its initiation is controlled by an expanded two-component signal transduction system, termed a phosphorelay. The Spo0A response regulator is the master control element in the decision to sporulate, consisting of a receiver or phosphoacceptor domain and an effector or transcription activation domain having a high degree of sequence conservation. This study examined long-term Spo0A protein evolution for determining whether concerted evolution or purifying selection was the major factor in its evolution. The proportion of synonymous (pS) and non-synonymous nucleotide (pN) differences between Spo0A genes from 28 bacterial species was analysed. DIVERGE software was used for evaluating site-specific amino acids before phylogenetic divergence between Bacillus and Clostridium for detecting site-selective constrains operating on the Spo0A protein. Evolutionary Trace software was used for tracing the phylogenetic markers for this protein.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional de Colombia. andresgutierrez@colombia.com
authors.forename: Andrés Julián Gutiérrez
authors.initials: AJ
authors.lastname: Escobar
authors.forename: Dolly Montoya
authors.initials: DM
authors.lastname: Castaño
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005075:Biological Evolution; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000378:metabolism; D030561:Databases, Nucleic Acid; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D012984:Software; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors