Colon cancer chemopreventive activities of pomegranate ellagitannins and urolithins.
Pomegranate juice derived ellagitannins and their intestinal bacterial metabolites, urolithins, inhibited TCDD-induced CYP1-mediated EROD activity in vitro with IC(50) values ranging from 56.7 microM for urolithin A to 74.8 microM for urolithin C. These compounds exhibited dose- and time-dependent decreases in cell proliferation and clonogenic efficiency of HT-29 cells. Inhibition of cell proliferation was mediated through cell cycle arrest in the G(0)/G(1) and G(2)/M stages of the cell cycle followed by induction of apoptosis. These results indicate that the ellagitannins and urolithins released in the colon upon consumption of pomegranate juice in considerable amounts could potentially curtail the risk of colon cancer development, by inhibiting cell proliferation and inducing apoptosis.
Kasimsetty
Sashi G
SG
Department of Pharmacognosy, Research Institute for Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA.
Bialonska
Dobroslawa
D
Reddy
Muntha K
MK
Ma
Guoyi
G
Khan
Shabana I
SI
Ferreira
Daneel
D
D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D001628:Beverages; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000502:physiology; D003110:Colonic Neoplasms / Q000473:pathology / Q000517:prevention & control*; D019073:HT29 Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D047348:Hydrolyzable Tannins / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000627:therapeutic use; D029561:Lythraceae / Q000737:chemistry*; D008751:Methylene Blue / Q000302:isolation & purification* / Q000627:therapeutic use; D011419:Propidium
D017304:Annexin A5; D047348:Hydrolyzable Tannins; D011419:Propidium; D008751:Methylene Blue