TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes.
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor complex. Each of the NF-Y subunits (NF-YA, NF-YB and NF-YC) in plants is encoded by multiple genes. Quantitative RT-PCR analysis revealed that five wheat NF-YC members (TaNF-YC5, 8, 9, 11 and 12) were upregulated by light in both the leaf and seedling shoot. Co-expression analysis of Affymetrix wheat genome array datasets revealed that transcript levels of a large number of genes were consistently correlated with those of the TaNF-YC11 and TaNF-YC8 genes in three to four separate Affymetrix array datasets. TaNF-YC11-correlated transcripts were significantly enriched with the Gene Ontology term photosynthesis. Sequence analysis in the promoters of TaNF-YC11-correlated genes revealed the presence of putative NF-Y complex binding sites (CCAAT motifs). Quantitative RT-PCR analysis of a subset of potential TaNF-YC11 target genes showed that ten out of the 13 genes were also light-upregulated in both the leaf and seedling shoot and had significantly correlated expression profiles with TaNF-YC11. The potential target genes for TaNF-YC11 include subunit members from all four thylakoid membrane-bound complexes required for the conversion of solar energy into chemical energy and rate-limiting enzymes in the Calvin cycle. These data indicate that TaNF-YC11 is potentially involved in regulation of photosynthesis-related genes.
Stephenson
Troy J
TJ
CSIRO Plant Industry, 306 Carmody Road, St. Lucia, QLD, 4067, Australia.
McIntyre
C Lynne
CL
Collet
Christopher
C
Xue
Gang-Ping
GP
D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D030561:Databases, Nucleic Acid; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010788:Photosynthesis / Q000235:genetics* / Q000528:radiation effects; D018515:Plant Leaves / Q000235:genetics / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000528:radiation effects; D011401:Promoter Regions, Genetic / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000528:radiation effects; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects; D015854:Up-Regulation / Q000235:genetics / Q000528:radiation effects*
D023081:CCAAT-Binding Factor; D010940:Plant Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; C402244:nuclear factor Y