Impact of selenium on NFκB translocation in isoproterenol-induced myocardial infarction in rats.
NFκB is a major transcription factor that controls the expression of various genes. Its activation is a complex process that can be triggered by many agents and one among them is reactive oxygen species. The aim of this study was to investigate the effect of selenium on NFκB activation in rats induced with myocardial infarction by isoproterenol (ISP). The markers of myocardial infarction showed increased activity in the serum of rats induced with ISP compared to the group that was pretreated with selenium along with ISP. Cellular selenium status was also found to be very low in the ISP-induced group of rats. The concentration of cytosolic NFκB was comparatively lower in the ISP group than in the group treated with selenium and ISP. Whereas higher levels of NFκB were found in the nuclear extract of the ISP-treated animals than in the selenium + ISP group. Elevated levels of malondialdehyde, hydroperoxides, and conjugated diens in the ISP-treated rats revealed the higher levels of oxidative stress in this group. Thus, our studies reveal the inhibitory effect of selenium in the nuclear translocation of NFκB during myocardial infarction. Histopathological studies of the heart also support the cardioprotective role of selenium.
Panicker
Seema
S
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.
Swathy
S S
SS
John
Febi
F
Madambath
Indira
I
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005260:Female; D007545:Isoproterenol; D009203:Myocardial Infarction / Q000139:chemically induced* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*
D000975:Antioxidants; D016328:NF-kappa B; D012643:Selenium; D007545:Isoproterenol