Effect of BMP-9 and its derived peptide on the differentiation of human white preadipocytes.
Several studies have shown that bone morphogenetic proteins (BMPs) can influence adipogenic and osteogenic cell lineages. We have shown that a peptide derived from BMP-9 (pBMP-9) at 400 ng/ml inhibits the proliferation of preosteoblasts and induces differentiation. We have now determined the effects of pBMP-9 (400 ng/ml) and equimolar concentrations of BMP-2 (100 ng/ml), BMP-9 (84.6 ng/ml) and pBMP-9 (9.04 ng/ml) on human white preadipocytes (HWP). pBMP-9 dose dependently reduced the proliferation of HWP without affecting the number of apoptotic cells. Incubation of the cells for 1 h with BMP-2, BMP-9 or pBMP-9 activated the Smad1/5/8 pathway, while incubation for 7 days in adipocyte differentiation (AD) serum-free medium containing ciglitazone and equimolar concentrations of BMP-2, BMP-9 or pBMP-9 enhanced the levels of mRNA of the adipogenic markers aP2 and adipoQ and increased the number of lipid vesicles. Thus, pBMP-9, like BMP-9, can increase the AD of HWP in AD serum-free medium.
Lord
E
E
Laboratory of Cell-Biomaterial Biohybrid Systems, Department of Chemical Engineering and Biotechnological Engineering, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, Québec, J1K 2R1, Canada. etienne.lord@usherbrooke.ca
Bergeron
E
E
Senta
H
H
Park
H
H
Faucheux
N
N
D052438:Adipocytes, White / Q000187:drug effects* / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects*; D000328:Adult; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005819:Genetic Markers; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D047495:PPAR gamma / Q000032:analysis; D010455:Peptides / Q000494:pharmacology; D051785:Smad Proteins / Q000187:drug effects; D045162:Thiazolidinediones / Q000494:pharmacology
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; C525887:GDF2 protein, human; D005819:Genetic Markers; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors; D047495:PPAR gamma; D010455:Peptides; D051785:Smad Proteins; D045162:Thiazolidinediones; C039671:ciglitazone