title: Chromosome integration of BAC (bacterial artificial chromosome): evidence of multiple rearrangements.
abstract: This paper reports our attempts to characterize transgene integration sites in transgenic mouse lines generated by the microinjection of large (from 30 to 145 kb) pig DNA fragments encompassing a mammary specific gene, the whey acidic protein gene (WAP). Among the various methods used, the thermal asymmetric interlaced (TAIL-) PCR method allowed us (1) to analyze transgene/genomic borders and internal concatamer junctions for eleven transgenic lines, (2) to obtain sequence information for seven borders, (3) to place three transgenes in the mouse genome, and (4) to obtain sequence data for seven transgene junctions in concatamers. Finally, we characterized various rearrangements in the borders and the inner parts of the transgene. The possibility of such complex rearrangements should be carefully considered when transgenic animals are produced with large genomic DNA fragments.
authors.affiliation: Biologie du Développement et Reproduction, UMR1198, INRA ENVA, 78352, Jouy-en-Josas, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Le Saux
authors.forename: Louis-Marie
authors.initials: LM
authors.lastname: Houdebine
authors.forename: Geneviève
authors.initials: G
authors.lastname: Jolivet
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D008845:Microinjections; D008894:Milk Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D008894:Milk Proteins; C024471:whey acidic proteins