title: Structure analysis of endosialidase NF at 0.98 A resolution.
abstract: Endosialidase NF (endoNF) is a bacteriophage-derived endosialidase that specifically degrades alpha-2,8-linked polysialic acid. The structure of a new crystal form of endoNF in complex with sialic acid has been refined at 0.98 A resolution. The 210 kDa homotrimeric multi-domain enzyme displays outstanding stability and resistance to SDS. Even at atomic resolution, only a minor fraction of side chains possess alternative conformations. However, multiple conformations of an active-site residue imply that it has an important catalytic function in the cleavage mechanism of polysialic acid.
authors.affiliation: Abteilung für Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
authors.forename: Eike C
authors.initials: EC
authors.lastname: Schulz
authors.forename: Piotr
authors.initials: P
authors.lastname: Neumann
authors.forename: Rita
authors.initials: R
authors.lastname: Gerardy-Schahn
authors.forename: George M
authors.initials: GM
authors.lastname: Sheldrick
authors.forename: Ralf
authors.initials: R
authors.lastname: Ficner
mesh_terms: D001435:Bacteriophages / Q000201:enzymology*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
chemical_list: C035355:endo-N-acetylneuraminidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid