title: Ekhidna lutea gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from the South East Pacific Ocean.
abstract: A novel aerobic, heterotrophic bacterium, designated BiosLi39(T), was isolated from the South East Pacific Ocean. Cells were Gram-negative gliding rods forming yellow colonies on marine agar. The isolate was oxidase-, catalase- and alkaline phosphatase-positive and β-galactosidase-negative. Strain BiosLi39(T) grew at 20-37°C (optimum 30°C), at pH7.0-9.0 (optimum pH8.0) and with 20-60 g NaCl l(-1) (optimum 30-50 g NaCl l(-1)). The fatty acids (>1 %) comprised iso-C(14 : 0), iso-C(15 : 1) G, iso-C(15 : 0), anteiso-C(15 : 0), C(15 : 1) G, C(15 : 0), iso-C(15 : 0) 2-OH, iso-C(16 : 1) G, iso-C(16 : 0), iso-C(16 : 0) 3-OH, iso-C(16 : 0) 2-OH, iso-C(17 : 0) 3-OH, C(17 : 0) 2-OH and three unidentified components with equivalent chain lengths of 17.87, 18.10 and 18.71. A significant proportion of the hydroxylated fatty acids are amide-linked. The lipid pattern indicated the presence of phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The strain contained menaquinone 7 as the sole respiratory lipoquinone and did not produce flexirubin-type pigments. The G+C content of the genomic DNA was 37.2&emsp14;mol%. Comparative 16S rRNA gene sequence analysis indicated that strain BiosLi39(T) was distantly related to all of the representatives of the phylum Bacteroidetes. Its closest relative was Marinoscillum furvescens IFO 15994(T), with which it shared 92.5 % 16S rRNA gene sequence similarity. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, we propose a novel genus and species, Ekhidna gen. nov., sp. nov., with type strain BiosLi39(T) (=DSM 19307(T) =CIP 109600(T) =OOB 398(T)).
authors.affiliation: Université Pierre et Marie Curie - Paris 6, CNRS, UMR7621, F-66650 Banyuls-sur-Mer, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Alain
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Tindall
authors.affiliation: Université Pierre et Marie Curie - Paris 6, CNRS, UMR7621, F-66650 Banyuls-sur-Mer, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Catala
authors.affiliation: Université Pierre et Marie Curie - Paris 6, CNRS, UMR7621, F-66650 Banyuls-sur-Mer, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Intertaglia
authors.affiliation: Université Pierre et Marie Curie - Paris 6, CNRS, UMR7621, F-66650 Banyuls-sur-Mer, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lebaron
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7