title: Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress.
abstract: OBJECTIVE
  To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes.
  
  
  METHODS
  Oxidative stress was produced through addition of hydrogen peroxide (H(2)O(2)) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H(2)O(2) (G H(2)O(2)), Group H(2)O(2) and KGF (G H(2)O(2)-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay.
  
  
  RESULTS
  The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001).
  
  
  CONCLUSIONS
  KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
authors.affiliation: Plastic Surgery Division, UNIFESP, Sao Paulo, Brazil. gragnani.dcir@epm.br
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
authors.forename: Marina Bertelli
authors.initials: MB
authors.lastname: Rossi
authors.forename: Janne Cely Medeiros
authors.initials: JC
authors.lastname: Albuquerque
authors.forename: Gabriela Soares Silva
authors.initials: GS
authors.lastname: Brito
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Aloise
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D006861:Hydrogen Peroxide; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor