Light modulated toxicity of isoproturon toward natural stream periphyton photosynthesis: a comparison between constant and dynamic light conditions.
This study tested if a variation in light intensity, in comparison to constant light required in well-designed toxicity test, could have measurable consequences on the sensitivity of phototrophic biofilms (periphyton) to isoproturon. Two independent experiments were carried out to investigate the combined effects of light and isoproturon on the photochemical behavior of intact natural biofilms by measurements of chlorophyll fluorescence and pigment composition. Experiment 1 consisted of exposing biofilms to series of isoproturon concentrations (0-2 mg L(-1)) for 7 h under constant light at different irradiance levels (25-300 micromol m(-2) s(-1)). In experiment 2, biofilms were exposed using more environmentally realistic conditions to three selected concentrations of isoproturon (2, 6 and 20 microg L(-1)) during a 7-h-simulated daily light cycle. Our results demonstrated that light, considered here as a direct physical stressor, slightly modulated the acute toxicity of isoproturon on these diatom dominated communities. This was attributed to the fact that these two factors act specifically on the photosynthetic activity. Furthermore, it was shown that a dynamic light regime increased periphyton sensitivity to isoproturon by challenging its photoprotective mechanisms such as the xanthophyll cycle, therefore implying that traditional ecotoxicological bioassays lead to underestimate the effect of isoproturon.
Laviale
Martin
M
Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille-Lille 1, F-59655 Villeneuve d'Ascq Cedex, France. martin.laviale@univ-nantes.fr
Prygiel
Jean
J
Créach
Anne
A
D018441:Biofilms / Q000187:drug effects*; D002734:Chlorophyll / Q000378:metabolism; D017377:Diatoms / Q000187:drug effects / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D005470:Fluorometry; D006540:Herbicides / Q000633:toxicity*; D010671:Phenylurea Compounds / Q000633:toxicity*; D017440:Photoperiod; D010788:Photosynthesis / Q000187:drug effects; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D006540:Herbicides; D010671:Phenylurea Compounds; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; C028904:isoproturon