Alterations in PNA binding of keratinocytes in oral keratosis.
Changes in the expression of peanut lectin (PNA) were examined in keratinocytes of oral keratosis showing a mixture of hyperortho- and hyperparakeratinized epithelium. In the hyperorthokeratinized epithelium, which was reacted with anti-filaggrin antibody in both granular and cornified cells, PNA bound to the surface of keratinocytes from the spinous layer to the granular layer. Neither anti-filaggrin nor PNA reactions were detected in keratinocytes of the hyperparakeratinized epithelium. After neuraminidase pretreatment, however, PNA staining appeared in all cells, except cornified cells, of both hyperortho- and hyperparakeratinized epithelia. These findings suggest that PNA-binding epitopes in keratinocytes were modified by sialic acid during the hyperparakeratotic process of oral keratosis.
Ohno
J
J
Pathology Section, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan. johno@college.fdcnet.ac.jp
Iwahashi
T
T
Ehara
M
M
Taniguchi
K
K
D004848:Epithelium / Q000378:metabolism; D000091344:Filaggrin Proteins; D006801:Humans; D007381:Intermediate Filament Proteins / Q000276:immunology; D015603:Keratinocytes / Q000378:metabolism*; D007972:Leukoplakia, Oral / Q000378:metabolism / Q000473:pathology; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000737:chemistry; D019887:Peanut Agglutinin / Q000378:metabolism*
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D019887:Peanut Agglutinin; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid