The protective effect of radicicol against renal ischemia--reperfusion injury in mice.
Overexpression of heat shock protein 70 kDa (HSP70) is known to confer cellular protection against ischemia-reperfusion (I/R) injury. Radicicol, a HSP90 inhibitor, has been reported to induce the expression of HSP70 protein. Here we studied whether radicicol attenuated renal I/R injury in vivo. Treatment of mice with radicicol ameliorated renal I/R injury and increased renal HSP70 mRNA and protein. Administration of radicicol with quercetin, an inhibitor of HSP70 induction, eliminated the renoprotective effect of radicicol. Our results suggest that the up-regulation of renal HSP70 protein by radicicol leads to a novel drug therapy against renal I/R injury.
Sonoda
Hiroko
H
Department of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Prachasilchai
Worapat
W
Kondo
Hiroaki
H
Yokota-Ikeda
Naoko
N
Oshikawa
Sayaka
S
Ito
Katsuaki
K
Ikeda
Masahiro
M
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D018942:Macrolides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011794:Quercetin / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control*; D015854:Up-Regulation / Q000187:drug effects
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D018942:Macrolides; D012333:RNA, Messenger; D011794:Quercetin; C035359:monorden