title: The role of MT2-MMP in cancer progression.
abstract: The role of MT2-MMP in cancer progression remains to be elucidated in spite of many reports on MT1-MMP. Using a human fibrosarcoma cell, HT1080 and a human gastric cancer cell, TMK-1, endogenous expression of MT1-MMP or MT2-MMP was suppressed by siRNA induction to examine the influence of cancer progression in vitro and in vivo. In HT1080 cells, positive both in MT1-MMP and MT2-MMP, the migration as well as the invasion was impaired by MT1-MMP or MT2-MMP suppression. Also cell proliferation in three dimensional (3D) condition was inhibited by MT1-MMP or MT2-MMP suppression and tumor growth in the nude mice transplanted with tumor cells were reduced either MT1-MMP or MT2-MMP suppression with a prolongation of survival time in vivo. MT2-MMP suppression induces more inhibitory effects on 3D proliferation and in vivo tumor growth than MT1-MMP. On the other hand, TMK-1 cells, negative in MT1-MMP and MMP-2 but positive in MT2-MMP, all the migratory, invasive, and 3D proliferative activities in TMK-1 are decreased only by MT2-MMP suppression. These results indicate MT2-MMP might be involved in the cancer progression more than or equal to MT1-MMP independently of MMP-2 and MT1-MMP.
authors.affiliation: Department of Molecular Pathology, Osaka University, Suita, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Ito
authors.forename: Ikuo
authors.initials: I
authors.lastname: Yana
authors.forename: Chisato
authors.initials: C
authors.lastname: Fujita
authors.forename: Aiko
authors.initials: A
authors.lastname: Irifune
authors.forename: Maki
authors.initials: M
authors.lastname: Takeda
authors.forename: Ayako
authors.initials: A
authors.lastname: Madachi
authors.forename: Seiji
authors.initials: S
authors.lastname: Mori
authors.forename: Yoshinosuke
authors.initials: Y
authors.lastname: Hamada
authors.forename: Naomasa
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D006801:Humans; D053517:Matrix Metalloproteinase 15 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000201:enzymology / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D053517:Matrix Metalloproteinase 15