[Saccharomyces boulardii reduced intestinal inflammation in mice model of 2,4,6-trinitrobencene sulfonic acid induced colitis: based on microarray].
OBJECTIVE
Saccharomyces boulardii has been reported to be beneficial in the treatment of inflammatory bowel disease. The aim of this work was to evaluate the effect of S. boulardii in a mice model of 2,4,6-trinitrobencene sulfonic acid (TNBS) induced colitis and analyze the expression of genes in S. boulardii treated mice by microarray.


METHODS
BALB/c mice received TNBS or TNBS and S. boulardii treatment for 4 days. Microarray was performed on total mRNA form colon, and histologic evaluation was also performed.


RESULTS
In mice treated with S. boulardii, the histological appearance and mortality rate were significantly restored compared with rats receiving only TNBS. Among 330 genes which were altered by both S. boulardii and TNBS (>2 folds), 193 genes were down-regulated by S. boulardii in microarray. Most of genes which were down-regulated by S. bouardii were functionally classified as inflammatory and immune response related genes.


CONCLUSIONS
S. boulardii may reduce colonic inflammation along with regulation of inflammatory and immune responsive genes in TNBS-induced colitis.
Lee
Sang Kil
SK
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. sklee@yuhs.ac
Kim
Hyo Jong
HJ
Chi
Sung Gil
SG
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000628:therapy*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D019936:Probiotics; D012440:Saccharomyces; D014302:Trinitrobenzenesulfonic Acid
D014302:Trinitrobenzenesulfonic Acid