title: Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores.
abstract: We present a novel method for integrating two single-molecule measurement modalities, namely, total internal reflection microscopy and electrical detection of biomolecules using nanopores. Demonstrated here is the electrical measurement of nanopore based biosensing performed simultaneously and in-sync with optical detection of analytes. This method makes it possible, for the first time, to visualize DNA and DNA-protein complexes translocating through a nanopore with high temporal resolution (1000 frames/s) and good signal to background. This paper describes a detailed experimental design of custom optics and data acquisition hardware to achieve simultaneous high resolution electrical and optical measurements on labeled biomolecules as they traverse through a approximately 4 nm synthetic pore. In conclusion, we discuss new directions and measurements, which this technique opens up.
authors.affiliation: Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
authors.forename: Gautam V
authors.initials: GV
authors.lastname: Soni
authors.forename: Alon
authors.initials: A
authors.lastname: Singer
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Yu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Sun
authors.forename: Ben
authors.initials: B
authors.lastname: McNally
authors.forename: Amit
authors.initials: A
authors.lastname: Meller
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D055615:Electrical Equipment and Supplies; D004867:Equipment Design; D008853:Microscopy / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D055096:Optical Devices; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
chemical_list: D011506:Proteins; D004247:DNA