title: Tailored adsorption of His6-tagged protein onto nickel(II)-cyclam grafted mesoporous silica.
abstract: The specific immobilisation of a histidine-tagged protein, Spi, onto mono-anchored nickel(II)-cyclam functionalised SBA-15 is reported.
authors.affiliation: Materials and Surface Science Institute, SFI-SRC in Solar Energy Conversion, Department of Chemical and Environmental Science, University of Limerick, Limerick, Ireland.
authors.forename: Darragh A
authors.initials: DA
authors.lastname: Gaffney
authors.forename: Sarah
authors.initials: S
authors.lastname: O'Neill
authors.forename: Mary C
authors.initials: MC
authors.lastname: O'Loughlin
authors.forename: Ulf
authors.initials: U
authors.lastname: Hanefeld
authors.forename: Jakki C
authors.initials: JC
authors.lastname: Cooney
authors.forename: Edmond
authors.initials: E
authors.lastname: Magner
mesh_terms: D000327:Adsorption; D001426:Bacterial Proteins / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D006571:Heterocyclic Compounds; C471213:His-His-His-His-His-His; D055767:Immobilized Proteins; D009842:Oligopeptides; C509969:SBA-15; C024560:cyclam; D006639:Histidine; D012822:Silicon Dioxide; D009532:Nickel