Mossy fiber sprouting, hippocampal damage and spontaneous recurrent seizures in pentylenetetrazole kindling rat model.
OBJECTIVE
The aim of this study was to determine the correlations among hippocampal damage, spontaneous recurrent seizures (SRS), and mossy fiber sprouting (MFS) using pentylenetetrazole (PTZ) kindling model.


METHODS
Chronic epileptic model was established by administration of PTZ. Behaviour and EEG seizure activity were recorded. Rats' hippocampus were analyzed with haematoxylin and eosin (H&E) stain for histological lesions and evaluated for MFS with Timm stain.


RESULTS
Prominent MFS was observed in area CA3 rather than the inner molecular layer in PTZ treated rats and the degree of MFS progressed with the development of behavioral kindled seizures. MFS preceded the occurrence of spontaneous seizures. No obvious neuronal necrosis and loss were observed in different regions of the hippocampus during kindling progression.


CONCLUSIONS
MFS is not the outcome of SRS. Severe hippocampal damage is not required in the development of MFS and SRS.
Tian
Fa-Fa
FF
Department of Neurology, Xiangya Hospital, Central South University, Changsha, China. tianfafa@gmail.com
Zeng
Chang
C
Guo
Ting-Hui
TH
Chen
Ying
Y
Chen
Jin-Mei
JM
Ma
Yun-Feng
YF
Fang
Jia
J
Cai
Xiao-Feng
XF
Li
Feng-Rong
FR
Wang
Xin-Hua
XH
Huang
Wen-Jiao
WJ
Fu
Jing-Jing
JJ
Dang
Jing
J
D000818:Animals; D004569:Electroencephalography; D006624:Hippocampus / Q000473:pathology* / Q000503:physiopathology; D007696:Kindling, Neurologic / Q000473:pathology*; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000473:pathology* / Q000503:physiopathology; D009474:Neurons / Q000473:pathology; D010433:Pentylenetetrazole / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000473:pathology* / Q000503:physiopathology; D013194:Staining and Labeling; D018709:Statistics, Nonparametric
D010433:Pentylenetetrazole