title: Longterm quiescent cells in the aged human subventricular neurogenic system specifically express GFAP-delta.
abstract: A main neurogenic niche in the adult human brain is the subventricular zone (SVZ). Recent data suggest that the progenitors that are born in the human SVZ migrate via the rostral migratory stream (RMS) towards the olfactory bulb (OB), similar to what has been observed in other mammals. A subpopulation of astrocytes in the SVZ specifically expresses an assembly-compromised isoform of the intermediate filament protein glial fibrillary acidic protein (GFAP-delta). To further define the phenotype of these GFAP-delta expressing cells and to determine whether these cells are present throughout the human subventricular neurogenic system, we analysed SVZ, RMS and OB sections of 14 aged brain donors (ages 74-93). GFAP-delta was expressed in the SVZ along the ventricle, in the RMS and in the OB. The GFAP-delta cells in the SVZ co-expressed the neural stem cell (NSC) marker nestin and the cell proliferation markers proliferating cell nuclear antigen (PCNA) and Mcm2. Furthermore, BrdU retention was found in GFAP-delta positive cells in the SVZ. In the RMS, GFAP-delta was expressed in the glial net surrounding the neuroblasts. In the OB, GFAP-delta positive cells co-expressed PCNA. We also showed that GFAP-delta cells are present in neurosphere cultures that were derived from SVZ precursors, isolated postmortem from four brain donors (ages 63-91). Taken together, our findings show that GFAP-delta is expressed in an astrocytic subpopulation in the SVZ, the RMS and the OB. Importantly, we provide the first evidence that GFAP-delta is specifically expressed in longterm quiescent cells in the human SVZ, which are reminiscent of NSCs.
authors.affiliation: Netherlands Institute for Neuroscience, Amsterdam, The Netherlands. berge@nin.knaw.nl <berge@nin.knaw.nl>
authors.forename: Simone A
authors.initials: SA
authors.lastname: van den Berge
authors.forename: Jinte
authors.initials: J
authors.lastname: Middeldorp
authors.forename: C Eleana
authors.initials: CE
authors.lastname: Zhang
authors.forename: Maurice A
authors.initials: MA
authors.lastname: Curtis
authors.forename: Brian W
authors.initials: BW
authors.lastname: Leonard
authors.forename: Diego
authors.initials: D
authors.lastname: Mastroeni
authors.forename: Pieter
authors.initials: P
authors.lastname: Voorn
authors.forename: Wilma D J
authors.initials: WD
authors.lastname: van de Berg
authors.forename: Inge
authors.initials: I
authors.lastname: Huitinga
authors.forename: Elly M
authors.initials: EM
authors.lastname: Hol
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D013234:Stem Cells / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D005904:Glial Fibrillary Acidic Protein