title: Preparation and immunological effectiveness of a Swine influenza DNA vaccine encapsulated in PLGA microspheres.
abstract: Optimal preparation conditions of DNA vaccine against swine influenza encapsulated in Poly (D,L)-lactic-co-glycolic acid (PLGA) microspheres were determined. The microspheres were prepared by an emulsion-evaporation method using PLGA as the biodegradable matrix forming polymer. Using the optimal preparation conditions, PLGA microspheres containing the DNA vaccine were produced with good morphology as evident from scanning electron micrographs, high encapsulation rate and high stability. The transfection test indicated that the vaccine could be expressed as an antigen in cells and maintained good bioactivity. Moreover, these results demonstrated that the PLGA microspheres containing DNA vaccine can be used to achieve prolonged release of plasmid DNA. These results have laid a foundation for further development before ultimate industrial application.
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Guo-Xin
authors.initials: GX
authors.lastname: Li
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Jin
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Wei
authors.forename: Qing-Shen
authors.initials: QS
authors.lastname: Sun
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Huang
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Tong
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D005260:Female; D019344:Lactic Acid / Q000737:chemistry*; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D004655:Emulsions; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA