title: High prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand.
abstract: OBJECTIVE
  To determine the prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand.
  
  
  METHODS
  Bacteria in stool specimens were screened for extended-spectrum beta-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates.
  
  
  RESULTS
  A markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M beta-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M beta-lactamase-producing bacteria were Escherichia coli (85.1%).
  
  
  CONCLUSIONS
  The study revealed the wide dissemination of CTX-M beta-lactamase-producing Enterobacteriaceae in the healthy population.
authors.affiliation: Department of Bioinformatics, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Sasaki
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Marie
authors.initials: M
authors.lastname: Niki
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakamura
authors.forename: Chalit
authors.initials: C
authors.lastname: Komalamisra
authors.forename: Wanna
authors.initials: W
authors.lastname: Maipanich
authors.forename: Teera
authors.initials: T
authors.lastname: Kusolsuk
authors.forename: Surapol
authors.initials: S
authors.lastname: Sa-Nguankiat
authors.forename: Somchit
authors.initials: S
authors.lastname: Pubampen
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases