title: [Oxidative stress of acute paraquat poisoned rats and sodium dimercaptopropane sulfonate intervention].
abstract: OBJECTIVE
  to study the oxidative stress of rats with acute paraquat poisoning and the intervention of Sodium Dimercaptopropane Sulfonate (NA-DMPS).
  
  
  METHODS
  Eighty male SD rats were randomizedly divided into: the normal control group (n=8), NA-DMPS control group (n=8), the PQ group (n=32, the rats were intraperitoneally injected with 1% PQ solution at the dosage of 20 mg/kg) and the NA-DMPS protected group (n=32). The rats in the groups of normal and NA-DMPS control were sacrificed 1d after administration of NS or NA-DMPS. And the rats in the PQ group and the NA-DMPS protected group were sacrificed at 6h, 1, 3, 7d after poisoning. Samples of serum, bronchoalveolar lavage fluid (BALF) and lung tissue were gathered. The MDA and CAT in serum, BALF and lung homogenate, the glutathione (GSH) in serum and BALF were measured. And the expression of Nuclear factor E2-related factor 2 (Nrf2) mRNA in lung was tested with RT-PCR.
  
  
  RESULTS
  Compared with the normal control group, the activities of MDA and CAT in serum, BALF and lung homogenate are higher in both groups of PQ and NA-DMPS protected. And compared with the PQ group, the activities of MDA in serum, BALF and lung homogenate of the NA-DMPS protected group decreased significantly at 6h, 1d after poisoning, whereas the activities of CAT are higher at 6h, 1, 3d in serum and 1, 3d in BALF and lung homogenate (P<0.05 or P<0.001). The serum GSH at 6h, 3d of the NA-DMPS protected group [(730.07 +/- 16.23), (793.66 +/- 7.40)] were higher than those in the PQ group. And the BALF GSH at 1, 3d of the NA-DMPS protected group [(609.75 +/- 6.74), (631.83 +/- 12.03)] were also markedly higher than the PQ group (P<0.05 or P<0.001). The expression of NRF2 mRNA of the lung at 1, 3, 7d in the PQ group [(0.71 +/- 0.061), (1.023 +/- 0.158), (0.969 +/- 0.046)] and the NA-DMPS protected group [(1.005 +/- 0.06), (1.464 +/- 0.166), (1.066 +/- 0.191)] were significantly higher than those in the control groups. Compared with the PQ group, the expression of NRF2 mRNA of the lung increased markedly in the NA-DMPS protected group at 1, 3d (P<0.01).
  
  
  CONCLUSIONS
  Na-DMPS decreases the activity of MDA and increases the activity of CAT, GSH and the expression of Nrf2 mRNA. NA-DMPS can protected rats from PQ intoxication by improving the balance of redox reaction.
authors.affiliation: Emergercy Medical Department, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: He
authors.forename: Guang-ju
authors.initials: GJ
authors.lastname: Zhao
authors.forename: Zhong-qiu
authors.initials: ZQ
authors.lastname: Lu
authors.forename: Guang-liang
authors.initials: GL
authors.lastname: Hong
authors.forename: Fei
authors.initials: F
authors.lastname: He
authors.forename: Huan
authors.initials: H
authors.lastname: Liang
authors.forename: Qiao-meng
authors.initials: QM
authors.lastname: Qiu
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000506:poisoning*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014494:Unithiol / Q000494:pharmacology*
chemical_list: D014494:Unithiol; D010269:Paraquat