Novel double-antigen sandwich immunoassay for human hepatitis B core antibody.
A novel diagnostic immunoassay testing procedure for hepatitis B virus core antibody (anti-HBc) using homogeneous purified full-length hepatitis B virus core antigen (HBcAg) capsids obtained from Escherichia coli was compared with Abbott Architect anti-HBc chemiluminescent microparticle immunoassay (CMIA; indirect method) against a library of specimens. A monoclonal anti-HBc neutralization confirmatory assay was then used to determine the degree of discordance between specimens. The new assay was found to be superior in both sensitivity and specificity.
Li
An
A
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science, Xiamen University, Xiamen, Fujian Province 361005, People's Republic of China.
Yuan
Quan
Q
Huang
Zhiyin
Z
Fan
Jian
J
Guo
Ruyi
R
Lou
Bin
B
Zheng
Qin
Q
Ge
Shengxiang
S
Chen
Yixin
Y
Su
Zhijun
Z
Yeo
Anthony E T
AE
Chen
Yu
Y
Zhang
Jun
J
Xia
Ningshao
N
D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000175:diagnosis* / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000097:blood*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens