title: Angiogenesis, fibrinogenesis and presence of synechiae after exeresis of a swine vocal fold mucosal microflap and use of topical mitomycin-C.
abstract: OBJECTIVE
  To evaluate swine vocal fold healing in a period of 30 days after topical mitomycin-C application.
  
  
  METHODS
  Twelve swine underwent exeresis of mucosal flaps from the free edge of the anterior third of both vocal folds with a cold instrument (laryngeal scissors). The animals were divided into two groups: EG (Experimental Group), consisting of 6 animals undergoing topical MMC application (4 mg/dL) on the operated area for 4 minutes; CG (Control Group), 6 animals undergoing topical saline solution application on the operated area for 4 minutes. After 30 days, the animals were sacrificed and the larynges were collected and examined for the presence of synechiae as well as a histological immunohistochemical assessment of immature and mature collagen deposition, number of blood vessels and myofibroblasts.
  
  
  RESULTS
  Mature collagen deposition in the EG was 452.12 microm(2) and 1332.31 microm(2) in the CG; immature collagen deposition was 1511.73 microm(2) in the EG and 1020.61 microm(2) in the CG. The number of myofibroblasts was 1.556 in the EG and 3.583 in the CG. The number of blood vessels was 2.565 in EG and 6.917 in the CG. There were no synechiae in the two studied groups.
  
  
  CONCLUSIONS
  There was an increase in immature collagen deposition in the experimental group when compared with the control group. There was a decrease in mature collagen deposition in the experimental group when compared with the control group. There was a decrease in the number of myofibroblasts in the experimental group when compared with the control group. A decrease in blood vessels was observed in the experimental group when compared with the control group. There was no synechia formation in either studied group.
authors.affiliation: Evangelic Faculty of Parana, University Evangelic Hospital, Curitiba, PR, Brazil. ipem@evangelico.org.br
authors.forename: Vinicius Ribas de Carvalho Duarte
authors.initials: VR
authors.lastname: Fonseca
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.forename: Jurandir Marcondes
authors.initials: JM
authors.lastname: Ribas Filho
authors.forename: Paulo Afonso Nunes
authors.initials: PA
authors.lastname: Nassif
authors.forename: Nicolau Gregori
authors.initials: NG
authors.lastname: Czeczko
authors.forename: Carmen Austrália Paredes
authors.initials: CA
authors.lastname: Marcondes
authors.forename: Marcelo Mazza do
authors.initials: MM
authors.lastname: Nascimento
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis / Q000145:classification; D005260:Female; D005337:Fibrin / Q000096:biosynthesis; D007820:Laryngeal Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D011897:Random Allocation; D013552:Swine; D000267:Tissue Adhesions; D014827:Vocal Cords / Q000098:blood supply* / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016685:Mitomycin; D005337:Fibrin; D003094:Collagen