High-resolution light microscopy using luminescent nanoparticles.
This review presents recent progress in the development of the luminescent nanoparticles for confocal and multiphoton microscopy. Four classes of nanomaterials are discussed: (1) silica-based nanoparticles doped with fluorescent molecules, (2) gold nanoparticles, (3) semiconductor nanocrystals (quantum dots/rods), and (4) nanophosphors. Special considerations are given to recently developed imaging nanoprobes, such as (1) organically modified silica (ORMOSIL) nanoparticles doped with two-photon absorbing fluorophores, which exhibit aggregation-enhanced fluorescence (AEF), and (2) nanophosphors (ceramic nanoparticles containing luminescent lanthanoid ions). Advantages and disadvantages of every class of nanomaterials and their specific applications are briefly discussed.
Ohulchanskyy
Tymish Y
TY
Institute for Lasers, Photonics and Biophotonics, SUNY at Buffalo, Buffalo, NY, USA. tyo2@buffalo.edu
Roy
Indrajit
I
Yong
Ken-Tye
KT
Pudavar
Haridas E
HE
Prasad
Paras N
PN
D005456:Fluorescent Dyes / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*
D005456:Fluorescent Dyes; D049408:Luminescent Agents