Tailored adsorption of His6-tagged protein onto nickel(II)-cyclam grafted mesoporous silica.
The specific immobilisation of a histidine-tagged protein, Spi, onto mono-anchored nickel(II)-cyclam functionalised SBA-15 is reported.
Gaffney
Darragh A
DA
Materials and Surface Science Institute, SFI-SRC in Solar Energy Conversion, Department of Chemical and Environmental Science, University of Limerick, Limerick, Ireland.
O'Neill
Sarah
S
O'Loughlin
Mary C
MC
Hanefeld
Ulf
U
Cooney
Jakki C
JC
Magner
Edmond
E
D000327:Adsorption; D001426:Bacterial Proteins / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D001426:Bacterial Proteins; D006571:Heterocyclic Compounds; C471213:His-His-His-His-His-His; D055767:Immobilized Proteins; D009842:Oligopeptides; C509969:SBA-15; C024560:cyclam; D006639:Histidine; D012822:Silicon Dioxide; D009532:Nickel