[Study on the immunogenicity of adeno-vector vaccine against H5N1 influenza A virus].
OBJECTIVE
To construct adenovirus vector vaccine against H5N1 influenza virus and study on the immunogenicity.


METHODS
In this study, we amplified hemagglutinin (HA) gene sequence of H5N1 influenza virus (A/Anhui/1/2005), then constructed an adenovirus vector vaccine (Adv-HA), followed by tests in BALB/c mice for the immunogenicity with the vaccine and immunization strategies.


RESULTS
The recombinate Adv-HA vaccine could effectively induce both humoral and cellular immunity against human H5N1 influenza virus.


CONCLUSIONS
The Adv-HA vaccination against H5N1 influenza is a potential strategy and worthy of further investigation.
Zhang
Xiao-guang
XG
Peking Union Medical College, Beijing 100730, China.
Li
Kui-biao
KB
Ma
Jing
J
Wang
Nai-fu
NF
Zhang
Xiao-mei
XM
Sang
Yun-hu
YH
Dong
Jie
J
Xu
Hong
H
Zeng
Yi
Y
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D002460:Cell Line; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D014611:Vaccination
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines