title: Different cross-reactivity of human and rodent sera to Tula virus and Puumala virus.
abstract: Tula virus (TULV) and Puumala virus (PUUV) are hantaviruses carried by the bank vole (Myodes glareolus) and European common vole (Microtus arvalis), respectively. PUUV is a causative agent of hemorrhagic fever with renal syndrome (HFRS), while TULV is thought to be apathogenic to humans. The N-terminal regions of the N proteins from TULV and PUUV were expressed and applied as enzyme-linked immunosorbent assay (ELISA) antigens. Colonized Japanese grass voles (Microtus montebelli) and BALB/c mice were used for experimental inoculation of the vole-borne hantaviruses TULV and PUUV. Voles and mice showed significant antibody production toward both viruses, but these antisera showed little cross-reactivity between TULV and PUUV in the immunofluorescence antibody assay and ELISA. In contrast, sera from patients with HFRS caused by PUUV exhibited high cross-reactivity against the TULV antigen, and sera from a natural rodent reservoir showed moderate cross-reactivity against the heterologous antigen, indicating that the antigenic cross-reactivity between TULV and PUUV differs in sera from rodents and humans.
authors.affiliation: Department of Laboratory Animal Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
authors.forename: Erdenesaikhan
authors.initials: E
authors.lastname: Tegshduuren
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Midori
authors.initials: M
authors.lastname: Taruishi
authors.forename: Rika
authors.initials: R
authors.lastname: Endo
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimizu
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koma
authors.forename: Shumpei P
authors.initials: SP
authors.lastname: Yasuda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ishihara
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D003411:Arvicolinae; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology* / Q000821:virology; D006801:Humans; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D029262:Puumala virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D012367:RNA, Viral
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D019590:Nucleocapsid Proteins; D012367:RNA, Viral