[Influence of immunodepressors on population level and adhesive characteristics of opportunistic bacteria in large intestine of white mice].
OBJECTIVE
To assess the effects of cyclophosphamide (CP), methotrexate (MT), and prednisolone (PS) on the levels of staphylococci, enterococci, Escherichia coli (EC) and lactose-negative enterobacteria (LNEB) in feces of white mice as well as to determine adhesive properties of isolated microorganisms.


METHODS
Drugs were administered intragastrically during 6 weeks; CP in dose 35.4 mg/kg daily; MT - 2.5 mg/kg over week; PS - 2.5 mg/kg daily; sterile distilled water was administered to controls. Microflora was assessed by common methods; adhesion of microorganisms to group 0 (I) human erythrocytes was determined.


RESULTS
Administration of prednisolone leads to increase of isolation rate of staphylococci, CP increased levels of LNEB and EC with suppressed enzymatic activity (P < 0.05). MT decreased isolation rate of typical EC (P < 0.05). MT also decreased degree of adhesion of EC and LNEB, whereas PS enhanced adhesiveness of LNEB, and CP decreased adhesion of staphylococci (P < 0.05). Statistically significant correlation between number and adhesiveness of enterobacteria was established (r = 0.73, P < 0.01).


CONCLUSIONS
Immunodepressors from different groups have non-similar effects on quantitative characteristics of microflora and adhesiveness of isolated opportunistic microorganisms.
Shitov
L N
LN
Romanov
V A
VA
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D015169:Colony Count, Microbial; D003520:Cyclophosphamide / Q000494:pharmacology; D016983:Enterococcus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007420:Intestine, Large / Q000382:microbiology*; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D011239:Prednisolone / Q000494:pharmacology; D040141:Staphylococcaceae / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology
D007166:Immunosuppressive Agents; D003520:Cyclophosphamide; D011239:Prednisolone; D008727:Methotrexate