Chemical analysis and screening as anticancer agent of anthocyanin-rich extract from Uva Caimarona ( Pourouma cecropiifolia Mart.) fruit.
The anthocyanin-rich extract (ARE) of the fruit from Pourouma cecropiifolia , a tropical plant native to the Amazon region, showed moderate cytotoxicity toward different cancer cell lines when evaluated by MTT assays. This extract was fractionated using Sephadex LH-20 chromatography to obtain three fractions (F1-F3), the composition of which was analyzed by HPLC-PDA and LC-ESI/MS. F1 was composed primarily of the monomeric anthocyanins delphinidin-3-O-beta-glucopyranoside, cyanidin-3-O-beta-glucopyranoside, and cyanidin-3-O-(6''-malonyl)glucopyranoside. F2 contained the isomeric flavonols quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-galactopyranoside and quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-glucopyranoside, the structures of which were confirmed by (1)H and (13)C NMR. F3 contained polymeric pigments, which were analyzed using tandem ESI/MS with an ion trap-TOF. The structures of two proanthocyanidin and two flavanol-anthocyanin condensed pigments were suggested on the basis of their MS(n) fragmentation patterns. After cell viability assays were performed, only fraction F3 showed a cell growth-inhibitory effect similar to the one found for ARE. F3 significantly reduced the viability of HEp-2 larynx, MKN-45 gastric carcinoma, and MCF-7 breast cancer cells; in contrast, the pure compounds did not show promising cytotoxicity toward the cancer cells evaluated.
Barrios
Juliana
J
Departamento de Química, Universidad Nacional de Colombia, Bogotá, 41012 Sevilla, Spain.
Cordero
Claudia Patricia
CP
Aristizabal
Fabio
F
Heredia
Francisco José
FJ
Morales
Alicia Lucía
AL
Osorio
Coralia
C
D000872:Anthocyanins / Q000302:isolation & purification* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001943:Breast Neoplasms; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D017753:Ecosystem; D029791:Ericaceae / Q000737:chemistry*; D005260:Female; D044948:Flavonols / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008113:Liver Neoplasms; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D014329:Tropical Climate
D000872:Anthocyanins; D000970:Antineoplastic Agents; D044948:Flavonols; D010936:Plant Extracts