title: Characterization of non-endcapped polymeric ODS column for the separation of triacylglycerol positional isomers.
abstract: The characteristics of a non-endcapped polymeric ODS column for the resolution of triacylglycerol positional isomers (TAG-PI) were examined using a recycle HPLC-atmospheric pressure chemical ionization/mass spectrometry system. A pair of TAG-PI containing saturated fatty acids at least 12 carbons was separated. Except for TAG-PI containing elaidic acid, pairs of TAG-PI containing three unsaturated fatty acids were not separated, even by recycle runs. These results indicate that the resolution of TAG-PI on a non-endcapped polymeric ODS stationary phase is realized by the recognition of the linear structure of the fatty acid and the binding position of the saturated fatty acid in TAG-PI. Chain length was also an important factor for resolution. This method may be a useful and simple for measuring the abundance ratio of TAG-PI containing saturated fatty acids in natural oils.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yuji
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.forename: Kenji
authors.initials: K
authors.lastname: Ichioka
authors.forename: Ikuma
authors.initials: I
authors.lastname: Kuroda
authors.forename: Noriko
authors.initials: N
authors.lastname: Noguchi
authors.forename: Shun
authors.initials: S
authors.lastname: Wada
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007536:Isomerism; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011108:Polymers; D014280:Triglycerides; C026679:octadecylsilica; D012822:Silicon Dioxide