Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test.
The new EU legislations for cosmetics (Seventh Amendment) have laid down deadlines for the replacement of animal tests in cosmetics. This policy stimulates the acceptance of in vitro approaches to test embryotoxic potentials of compounds in cosmetics products. The embryonic stem cell test (EST) designed by The European Centre for the Validation of Alternative Methods (ECVAM) is currently the most promising in vitro assay to predict the embryotoxic potential of compounds. In this study, six selected compounds (hydroquinone, eugenol, dibutyl phthalate, antimony (III) oxide, neodymium (III) nitrate hydrate, melamine) formerly involved in cosmetic products were selected to test their embryotoxic potentials by the EST. 5-Fluorouracil and penicillin G were separately set as positive and negative control. The embryotoxic potential was determined by the prediction model (PM), which was calculated from three endpoints, the IC(50) 3T3, IC(50) ES, and ID(50). Hydroquinone, eugenol, and antimony (III) oxide indicated with strong embryotoxicity, while dibutyl phthalate, neodymium (III) nitrate hydrate, and melamine exhibited a weak embryotoxicity. These results may provide a valuable attempt to expand the application of EST to the cosmetics field.
Chen
Rui
R
Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, 74# Zhongshan Road 2, Guangzhou, Guangdong. PR China 510080.
Chen
Jing
J
Cheng
Shujun
S
Qin
Jie
J
Li
Weiqiang
W
Zhang
Lirong
L
Jiao
Hong
H
Yu
Xinbing
X
Zhang
Xiuming
X
Lahn
Bruce T
BT
Xiang
Andy Peng
AP
D000818:Animals; D000965:Antimony / Q000633:toxicity; D002460:Cell Line; D003358:Cosmetics / Q000633:toxicity*; D003993:Dibutyl Phthalate / Q000633:toxicity; D053595:Embryonic Stem Cells / Q000187:drug effects*; D005054:Eugenol / Q000633:toxicity; D006873:Hydroquinones / Q000633:toxicity; D051379:Mice; D009354:Neodymium / Q000633:toxicity; D010087:Oxides / Q000633:toxicity; D013723:Teratogens / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*; D014227:Triazines / Q000633:toxicity
D003358:Cosmetics; D006873:Hydroquinones; D010087:Oxides; D013723:Teratogens; D014227:Triazines; C091696:antimony oxide; D003993:Dibutyl Phthalate; D009354:Neodymium; D005054:Eugenol; D000965:Antimony; C011907:melamine; C031927:hydroquinone