Echinacoside retards cellular senescence of human fibroblastic cells MRC-5.
In this study, effects of echinacoside, one of the phenylethanoids isolated from the stems of Cistanches salsa, a Chinese traditional herbal medicine, on human embryo lung fibroblastic MRC-5 cells, was investigated. Activity of cell proliferation was evaluated with Alamar Blue, showing that treatment with echinacoside could retard the senescence. Flow cytometry results show that echinacoside could trigger cells in the G1 phase to enter the S phase and G2 phase, and could improve ROS degradation. The results from comet assay indicate that echinacoside could protect cells from DNA damage, partly elucidating the mechanism of its effects. All of the above results suggest that echinacoside has potential anti-senescence activity.
Xie
Hong
H
Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Medicine, Tsinghua University, Beijing, China.
Zhu
Hui
H
Cheng
Cong
C
Liang
Yu
Y
Wang
Zhao
Z
D002460:Cell Line; D002470:Cell Survival; D016922:Cellular Senescence / Q000187:drug effects*; D031671:Cistanche; D004396:Coloring Agents; D020552:Comet Assay; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016196:S Phase / Q000187:drug effects
D004396:Coloring Agents; D006027:Glycosides; D017382:Reactive Oxygen Species; C060297:echinacoside