title: Mast cells in canine cutaneous hemangioma, hemangiosarcoma and mammary tumors.
abstract: Mast cell count (MCC) in 45 dogs with cutaneous hemangioma (HA, n = 12), hemangiosarcoma (HSA, n = 12), mammary adenoma (AD, n = 9) and mammary adenocarcinoma (AC, n = 12) was made using Toluidine blue stained sections. Antibodies against endothelial cell markers, Factor VIII and VEGF were used to visualize and determine the hot spot micro-vessel density (MVD). Total MCC and MCC along the invasive edges were significantly higher (p < 0.001) in canine mammary AC than in AD. The total MCC did not significantly differ (p > 0.05), in HSAs (8.6 +/- 3.3) than in HAs (5.5 +/- 2.8). There is a positive correlation (r = 0.14) between the hot spot MCC and MVD in mammary AC, although not significant (p = 0.3172), indicating that mast cells are associated with angiogenesis in canine mammary AC. This study suggests that mast cells may play an important role in neovascularization of canine cutaneous vascular and mammary neoplasms. Detailed studies encompassing correlation of MCC and MVD with clinical outcomes and prognosis in these neoplasms are recommended.
authors.affiliation: Department of Pathology, Tifton Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, 43 Brighton Rd, Tifton, GA, 31793, USA. mwoldem@uga.edu
authors.forename: Moges
authors.initials: M
authors.lastname: Woldemeskel
authors.forename: Sreekumari
authors.initials: S
authors.lastname: Rajeev
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply / Q000473:pathology / Q000662:veterinary; D000236:Adenoma / Q000098:blood supply / Q000473:pathology / Q000662:veterinary; D000818:Animals; D002452:Cell Count / Q000662:veterinary; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005169:Factor VIII / Q000378:metabolism; D005260:Female; D006391:Hemangioma / Q000098:blood supply / Q000473:pathology / Q000662:veterinary*; D006394:Hemangiosarcoma / Q000098:blood supply / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D015674:Mammary Neoplasms, Animal / Q000098:blood supply / Q000473:pathology*; D008407:Mast Cells / Q000473:pathology*; D008415:Mastocytosis / Q000098:blood supply / Q000473:pathology / Q000662:veterinary*; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D012878:Skin Neoplasms / Q000098:blood supply / Q000473:pathology / Q000662:veterinary*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D005169:Factor VIII