title: Crystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1 from Encephalitozoon cuniculi.
abstract: Transcription elongation by eukaryotic RNA polymerase II requires the coupling of mRNA synthesis and mRNA processing and export. The essential protein Iws1 is at the interface of these processes through its interaction with histone chaperone and elongation factor Spt6 as well as with complexes involved in mRNA processing and export. Upon crystallization of the evolutionarily conserved domain of Iws1 from Encephalitozoon cuniculi, four different crystal forms were obtained. Three of the crystal forms belonged to space group P2(1) and one belonged to space group P222(1). Preliminary X-ray crystallographic analysis of one of the crystal forms allowed the collection of data to 2.5 A resolution.
authors.affiliation: IGBMC (Institut de Génétique et Biologie Moléculaire et Cellulaire), Département de Biologie et Génomique Structurales, UDS, CNRS, INSERM, 1 Rue Laurent Fries, BP 10142, 67404 Illkirch CEDEX, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Koch
authors.forename: Marie Laure
authors.initials: ML
authors.lastname: Diebold
authors.forename: Jean
authors.initials: J
authors.lastname: Cavarelli
authors.forename: Christophe
authors.initials: C
authors.lastname: Romier
mesh_terms: D018360:Crystallography, X-Ray; D009670:Encephalitozoon cuniculi / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D035602:Transcriptional Elongation Factors / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D035602:Transcriptional Elongation Factors