title: Short communication: characterization of a monoclonal antibody for kappa-casein B of cow's milk.
abstract: A monoclonal antibody (antik-B) against an oligopeptide of 23 AA corresponding to the region 131-153 of bovine kappa-casein (kappa-CN) B was generated using the Human Combinatorial Antibody Library (HuCAL) technology. Both AA substitutions distinguishing kappa-CN A and B are located in that region (positions 136 and 148). In this study, the reactivity of antik-B to milk samples collected from cows previously genotyped as CSN3*AA, CSN3*AB, and CSN3*BB was tested. According to Western blot results, antik-B recognized kappa-CN B and it showed no cross-reactivity toward kappa-CN A and other milk proteins. Furthermore, a modified Western blot method, urea-PAGE Western blot, was set up to assess the reactivity of antik-B toward all isoforms of kappa-CN B. In conclusion, antik-B was specific to kappa-CN B in milk and it seemed to be reactive toward all its isoforms.
authors.affiliation: Department of Animal Production, Veterinary Biotechnologies, Food Quality and Safety, University of Parma, via del Taglio 10, 43126 Parma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Summer
authors.forename: E
authors.initials: E
authors.lastname: Santus
authors.forename: L
authors.initials: L
authors.lastname: Casanova
authors.forename: H
authors.initials: H
authors.lastname: Joerg
authors.forename: A
authors.initials: A
authors.lastname: Rossoni
authors.forename: C
authors.initials: C
authors.lastname: Nicoletti
authors.forename: G
authors.initials: G
authors.lastname: Donofrio
authors.forename: P
authors.initials: P
authors.lastname: Mariani
authors.forename: M
authors.initials: M
authors.lastname: Malacarne
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002364:Caseins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005838:Genotype; D008892:Milk / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D002364:Caseins