Effects of metformin on serum levels of sex hormone, leptin and insulin in ovariectomized Sprague-Dawley rats.
The aim of the present experiment was to study the effects of metformin (MF) on serum concentrations of sex hormone, leptin and insulin in ovariectomized (OVX) Sprague-Dawley (SD) rats. The serum estradiol (E2), progesterone (P), leptin and insulin concentrations were measured with radioimmunoassay (RIA). Compared with SHAM operated rats, the serum concentrations of E2 and P were significantly lower (P < 0.01), but leptin and insulin were significantly higher in OVX rats (P < 0.01). MF (135mg kg(-1) d(-1), ig, 30 d) had no evident effects on serum concentrations of E2, P, leptin and insulin in OVX rats. MF (270 mg kg(-1) d(-1), ig, 30 d) markedly increased serum concentrations of E2 and P (P < 0.05) while decreased serum concentrations of leptin and insulin (P < 0.05 and P < 0.01) in OVX rats. We concluded that long-term treatment of high dose MF cannot only significantly decrease serum concentrations of leptin and insulin, but also increase E2 and P in OVX SD rats.
Yan
Li
L
Department of Pharmacology, Weifang Medical College, Weifang, P R China.
Kang
Bai
B
Li
Guangzhou
G
Yin
Zhaozhen
Z
Wang
Yanyan
Y
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008687:Metformin / Q000494:pharmacology*; D010052:Ovariectomy; D011374:Progesterone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
D012739:Gonadal Steroid Hormones; D007004:Hypoglycemic Agents; D007328:Insulin; D020738:Leptin; D011374:Progesterone; D004958:Estradiol; D008687:Metformin