Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as HPLC stationary phases.
Molecularly imprinted polymers (MIPs) of poly(ethylene glycol dimethacrylate) and poly(N-isopropylacrylamide-co-ethylene glycol dimethacrylate) were synthesized for the first time in supercritical carbon dioxide (scCO(2)), using Boc-L-tryptophan as template. Supercritical fluid technology provides a clean and one-step synthetic route for the preparation of affinity polymeric materials with sensing capability for specific molecules. The polymeric materials were tested as stationary HPLC phases for the enantiomeric separation of L- and D-tryptophan. HPLC results prove that the synthesized MIPs are able to recognize the template molecule towards its enantiomer which opens up potential applications in chromatographic chiral separation.
da Silva
Mara Soares
MS
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Vão
Eva R
ER
Temtem
Márcio
M
Mafra
Luís
L
Caldeira
Jorge
J
Aguiar-Ricardo
Ana
A
Casimiro
Teresa
T
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007536:Isomerism; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D011108:Polymers / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014364:Tryptophan / Q000737:chemistry*
D011108:Polymers; D014364:Tryptophan