Effect of amnion-derived cellular cytokine solution on healing of experimental partial-thickness burns.
BACKGROUND
Amnion-derived multipotent progenitor (AMP) cells, unlike most stem cells, have been demonstrated to be nontumorigenic and nonimmunogenic. Amnion-derived cellular cytokine solution (ACCS), a secreted product of AMP cells, is a cocktail of cytokines existing at physiological levels and has been used to accelerate epithelialization of experimental partial-thickness burns.


METHODS
Using modifications of Zawacki's guinea pig partial-thickness scald burn model, a total of 65 animals were treated with ACCS, ACCS + AMP cells, unconditioned medium (UCM) + AMP cells, or either UCM alone or saline as controls. Dosage times ranged from every other day to once a week. Percent epithelialization was serially determined from acetate wound tracings. Histology was performed on wound biopsies.


RESULTS
ACCS, UCM + AMP cells, and ACCS + AMP cells improved epithelialization compared with the two control groups (P < 0.05). When ACCS was delivered more frequently, statistically significant more rapid epithelialization occurred (P < 0.05). By day 7, all groups treated with ACCS had reached at least 90% epithelialization, whereas control groups were only 20-40% epithelialized (P < 0.05). Histology showed excellent regeneration of the epidermis with rete ridge formation. Hair growth occurred in ACCS-treated animals but not in the control group.


CONCLUSIONS
Amnion-derived cellular cytokine solution accelerates the healing of experimental partial-thickness burns. Based on these findings, a multicenter clinical trial is underway.
Payne
Wyatt G
WG
Institute for Tissue Regeneration, Repair, and Rehabilitation, Bay Pines Veterans Healthcare System, Bay Pines, FL, USA.
Wachtel
Thomas L
TL
Smith
Charlotte A
CA
Uberti
M Georgina
MG
Ko
Francis
F
Robson
Martin C
MC
D000279:Administration, Cutaneous; D000650:Amnion / Q000166:cytology*; D000818:Animals; D002056:Burns / Q000188:drug therapy / Q000601:surgery*; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D008297:Male; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000637:transplantation*; D033581:Stem Cell Transplantation; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
D016207:Cytokines