title: The expression of IFN-gamma, IL-4, Foxp3 and perforin genes are not correlated with DNA methylation status in patients with immune thrombocytopenic purpura.
abstract: We investigated the relationship between the expression of IFN-gamma, IL-4, Foxp3 and perforin gene and the methylation status of their promoters in immune thrombocytopenic purpura (ITP) patients. Gene expression and DNA methylation were determined by quantitative PCR and bisulfite genomic sequencing, respectively. The expression of IFN-gamma was higher, while the expression of IL-4 was notably lower in ITP patients when compared with the controls. Most importantly, the Th1/Th2 (IFN-gamma/IL-4) was remarkably higher in ITP patients, showing that the ITP patients were mainly in the Th1 polarization response. Although the methylation rate of CpGs located at -409 of perforin was higher (p = 0.0376) and that of IFN-gamma were relatively higher (p = 0.0582) in ITP, we did not find the negative correlation between the expression of those genes and the methylation of their promoters.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
authors.forename: Weiting
authors.initials: W
authors.lastname: Du
authors.forename: Donghai
authors.initials: D
authors.lastname: Wang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.forename: Tao
authors.initials: T
authors.lastname: Sui
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054353:Perforin; D011401:Promoter Regions, Genetic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D055815:Young Adult
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D054353:Perforin; D015847:Interleukin-4; D007371:Interferon-gamma