Expression of receptor for activated C kinase 1 in healing skin and mucosal wounds.
Postnatal (adult) mammalian wound healing results in the formation of scar, whereas fetal mammals are able to effect wound repair without scar. We have investigated the expression pattern of the receptor of activated C kinase 1 (RACK1), a pleiotropic G-protein-like molecule, in healing skin and mucosal wounds in a rabbit model after obtaining a full-length clone of the rabbit RACK1 cDNA. In both adult skin and mucosal wounds, RACK1 mRNA expression is decreased relative to unwounded controls. In contrast, in fetal skin wounds RACK1 expression is unaltered from fetal control. Fibroblasts derived from adult skin tissue express more RACK1 message than fetal skin fibroblasts. These observations suggest that RACK1 may play an important role in distinguishing scarless fetal wound healing from its scirrhous counterpart in adults.
Satish
Latha
L
Wound Healing Program, Center for Genomic Sciences, Allegheny-Singer Research Institute, 320 East North Ave., Pittsburgh, PA 15212, USA.
Hogg
Justin
J
Johnson
Sandra
S
Oswald
Duane
D
Post
J Christopher
JC
Ehrlich
Garth D
GD
Kathju
Sandeep
S
D000367:Age Factors; D000818:Animals; D002610:Cheek; D005333:Fetus / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D009061:Mouth Mucosa / Q000293:injuries / Q000503:physiopathology; D011493:Protein Kinase C / Q000378:metabolism* / Q000502:physiology; D011817:Rabbits; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries*; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011493:Protein Kinase C