title: Lymphoid tyrosine phosphatase R620W variant and inflammatory bowel disease in Tunisia.
abstract: OBJECTIVE
  To assess the possible association between PTPN22 (R620W) gene polymorphism and inflammatory bowel disease (IBD).
  
  
  METHODS
  One hundred and sixty-four patients with IBD [105 Crohn's disease (CD) and 59 ulcerative colitis (UC)] and 100 healthy controls were recruited. Genotyping of the PTPN22 gene 1858C-->T polymorphism was performed by restriction fragment length polymorphism-polymerase chain reaction with RsaI digestion.
  
  
  RESULTS
  The genotypic and allelic frequencies of (R620W) PTPN22 gene polymorphism reveal a significant association of the PTPN22 620-W allele with IBD, compared to the healthy control group (OR: 17.81, 95% CI: 4.18-21.86, P = 0.00001). Nevertheless, no difference in this polymorphism was found between CD and UC patients. No significant association was found between the frequencies of genotypes of the PTPN22 gene with either the clinical features such as sex, age, age at disease onset, and extent of colitis, or the production of serological markers (anti-Saccharomyces cerevisiae antibody in CD and perinuclear anti-neutrophil cytoplasmic antibody in UC).
  
  
  CONCLUSIONS
  These observations confirm the association of IBD susceptibility with the PTPN22 1858T (620-W) allele in Tunisian patients.
authors.affiliation: Laboratory of Immunology, Charles Nicolle Hospital, Boulevard 9 Avril, Tunis 1006, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Sfar
authors.forename: Walid
authors.initials: W
authors.lastname: Ben Aleya
authors.forename: Leila
authors.initials: L
authors.lastname: Mouelhi
authors.forename: Houda
authors.initials: H
authors.lastname: Aouadi
authors.forename: Thouraya
authors.initials: T
authors.lastname: Ben Rhomdhane
authors.forename: Mouna
authors.initials: M
authors.lastname: Makhlouf
authors.forename: Salwa
authors.initials: S
authors.lastname: Ayed-Jendoubi
authors.forename: Houda
authors.initials: H
authors.lastname: Gargaoui
authors.forename: Taoufik
authors.initials: T
authors.lastname: Najjar
authors.forename: Taieb
authors.initials: T
authors.lastname: Ben Abdallah
authors.forename: Khaled
authors.initials: K
authors.lastname: Ayed
authors.forename: Yousr
authors.initials: Y
authors.lastname: Gorgi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics*; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054596:Protein Tyrosine Phosphatase, Non-Receptor Type 22 / Q000235:genetics*; D014416:Tunisia; D055815:Young Adult
chemical_list: C516765:PTPN22 protein, human; D054596:Protein Tyrosine Phosphatase, Non-Receptor Type 22