title: miR-146a induces differentiation of periodontal ligament cells.
abstract: Differentiation of periodontal ligament (PDL) cells occurs under specific induction; furthermore, NF-kappaB signaling is important for regulation of bone differentiation. MicroRNAs are small non-coding RNAs that repress the translation of target genes and modulate cellular processes. miR-146a has been reported to modulate NF-kappaB signaling. This study hypothesized that miR-146a has a regulatory role in PDL differentiation by affecting NF-kappaB signaling. Immortalized PDL (I-PDL) cell lines were established by exogenous telomerase expression. The genesis of alkaline phosphatase and the up-regulation of miR-146a were induced by ascorbic acid in the I-PDL cells and primary PDL cells. I-PDL cells with exogenous miR-146a expression showed attenuation of NF-kappaB activity and exhibited higher differentiation relative to the controls. Exogenous NF-kappaB expression decreased the expression of differentiation markers, while the inactivation of endogenous NF-kappaB increased alkaline phosphatase in I-PDL cells. This study concludes that miR-146a promotes the differentiation in PDL cells through the down-regulation of NF-kappaB signaling.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, Taipei, Taiwan.
authors.forename: P-S
authors.initials: PS
authors.lastname: Hung
authors.forename: F-C
authors.initials: FC
authors.lastname: Chen
authors.forename: S-H
authors.initials: SH
authors.lastname: Kuang
authors.forename: S-Y
authors.initials: SY
authors.lastname: Kao
authors.forename: S-C
authors.initials: SC
authors.lastname: Lin
authors.forename: K-W
authors.initials: KW
authors.lastname: Chang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D015536:Down-Regulation / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D000469:Alkaline Phosphatase