An effective method for the quantitative detection of porcine endogenous retrovirus in pig tissues.
Xenotransplantation shows great promise for providing a virtually limitless supply of cells, tissues, and organs for a variety of therapeutical procedures. However, the potential of porcine endogenous retrovirus (PERV) as a human-tropic pathogen, particularly as a public health risk, is a major concern for xenotransplantation. This study focus on the detection of copy number in various tissues and organs in Banna Minipig Inbreed (BMI) from 2006 to 2007 in West China Hospital, Sichuan University. Real-time quantitative polymerase chain reaction (SYBR Green I) was performed in this study. The results showed that the pol gene had the most copy number in tissues compared with gag, envA, and envB. Our experiment will offer a rapid and accurate method for the detection of the copy number in various tissues and was especially suitable for the selection of tissues or organs in future clinical xenotransplantation.
Zhang
Peng
P
Department of Urology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
Yu
Ping
P
Wang
Wei
W
Zhang
Li
L
Li
Shengfu
S
Bu
Hong
H
D000818:Animals; D020077:Endogenous Retroviruses / Q000235:genetics / Q000472:pathogenicity*; D018628:Gene Dosage / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013552:Swine; D013556:Swine, Miniature / Q000821:virology*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous / Q000009:adverse effects