Screening of DNA aptamer which binds to alpha-synuclein.
Alpha-synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against alpha-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the alpha-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to alpha-synuclein. Moreover, M5-15 specifically binds to the alpha-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in alpha-synuclein analysis and diagnosis.
Tsukakoshi
Kaori
K
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Harada
Ryuichi
R
Sode
Koji
K
Ikebukuro
Kazunori
K
D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D011485:Protein Binding; D051844:alpha-Synuclein / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D051844:alpha-Synuclein