Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells.
A small stem-like subpopulation was isolated from human hepatocellular carcinoma (HCC) MHCC97 cells, characterized by their high efflux ability of the Hoechst 33342 dye. These side population (SP) cells were able to generate a heterogeneous mixture of SP and main population (MP) cells, while the MP cells rarely generated SP cells. Cell cycle analysis also revealed that more SP cells were in the G0 phase. They express higher levels of BCRP1, AFP and CK19 than MP cells. SP cells showed significantly higher viability than MP cells following treatment with doxorubicin or methotrexate. Actin polymerization and migration assays indicate that SP cells have a higher migration capacity and in vivo tumorigenicity of these cells is also higher. Collectively, we conclude that the SP is an enriched source of stem-like cells and may be an effective target for therapy and a useful tool to investigate the HCC tumorigenic and metastatic process.
Zhang
Ning
N
Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi Province 710032, P.R. China.
Li
Ren
R
Tao
Kai-Shan
KS
Cao
Da-Yong
DY
Ti
Zhen-Yu
ZY
Ding
Rui
R
Cai
Lei
L
Zhang
Fu-Qin
FQ
Dou
Ke-Feng
KF
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D016764:Cell Polarity; D054377:Chemokine CXCL12 / Q000502:physiology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000473:pathology*
D054377:Chemokine CXCL12