title: LAMP using a disposable pocket warmer for anthrax detection, a highly mobile and reliable method for anti-bioterrorism.
abstract: A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that uses a disposable pocket warmer without the need for electricity (pocket-warmer LAMP). In our tests, the detection limit of the pocket-warmer LAMP was 1,000 copies of Bacillus anthracis pag and capB gene fragments per tube. The pocket-warmer LAMP also detected B. anthracis genes from DNA extracted from 0.1 volume of a B. anthracis colony. The lower detection limit of the pocket-warmer LAMP was not significantly different from that of a conventional LAMP using a heat block, and was not changed under cold (4 degrees C) or warm (37 degrees C) conditions in a Styrofoam box. The pocket-warmer LAMP could be useful against bioterrorism, and as a sensitive, reliable detection tool in areas with undependable electricity infrastructures.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
authors.forename: Ben
authors.initials: B
authors.lastname: Hatano
authors.forename: Takayuki
authors.initials: T
authors.lastname: Maki
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Obara
authors.forename: Hitomi
authors.initials: H
authors.lastname: Fukumoto
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hagisawa
authors.forename: Yoshitaro
authors.initials: Y
authors.lastname: Matsushita
authors.forename: Akiko
authors.initials: A
authors.lastname: Okutani
authors.forename: Boldbaastar
authors.initials: B
authors.lastname: Bazartseren
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D023482:Bioterrorism; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C030325:anthrax toxin