Endocytosis is required for efficient apical constriction during Xenopus gastrulation.
Coordinated apical constriction (AC) in epithelial sheets drives tissue invagination [1, 2] and is required for diverse morphogenetic movements such as gastrulation [3], neurulation [4, 5], and organogenesis [6]. We showed previously that actomyosin contractility drives AC in Xenopus laevis bottle cells [7]; however, it remained unclear whether it does so in concert with other processes. Here we report that endocytosis-driven membrane remodeling is required for efficient AC. We found endosomes exclusively in bottle cells in the early gastrula. Disrupting endocytosis with dominant-negative dynamin or rab5 perturbed AC, with a significant decrease in constriction rate late in the process, suggesting that endocytosis operates downstream of actomyosin contractility to remove excess membrane. Additionally, disrupting endocytosis during neurulation inhibits AC in hingepoint cells, resulting in neural tube closure defects. Thus, membrane remodeling during AC could be a general mechanism to achieve efficient invagination in embryos.
Lee
Jen-Yi
JY
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. jenyilee@berkeley.edu <jenyilee@berkeley.edu>
Harland
Richard M
RM
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D046149:Embryo Culture Techniques; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000502:physiology; D054262:Gastrulation / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000502:physiology; D009024:Morphogenesis; D054259:Neural Tube / Q000196:embryology; D014982:Xenopus laevis / Q000196:embryology* / Q000502:physiology*