title: High-resolution light microscopy using luminescent nanoparticles.
abstract: This review presents recent progress in the development of the luminescent nanoparticles for confocal and multiphoton microscopy. Four classes of nanomaterials are discussed: (1) silica-based nanoparticles doped with fluorescent molecules, (2) gold nanoparticles, (3) semiconductor nanocrystals (quantum dots/rods), and (4) nanophosphors. Special considerations are given to recently developed imaging nanoprobes, such as (1) organically modified silica (ORMOSIL) nanoparticles doped with two-photon absorbing fluorophores, which exhibit aggregation-enhanced fluorescence (AEF), and (2) nanophosphors (ceramic nanoparticles containing luminescent lanthanoid ions). Advantages and disadvantages of every class of nanomaterials and their specific applications are briefly discussed.
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, SUNY at Buffalo, Buffalo, NY, USA. tyo2@buffalo.edu
authors.forename: Tymish Y
authors.initials: TY
authors.lastname: Ohulchanskyy
authors.forename: Indrajit
authors.initials: I
authors.lastname: Roy
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
authors.forename: Haridas E
authors.initials: HE
authors.lastname: Pudavar
authors.forename: Paras N
authors.initials: PN
authors.lastname: Prasad
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D049408:Luminescent Agents