title: Halogenated DOPA in a Marine Adhesive Protein.
abstract: The sandcastle worm Phragmatopoma californica, a marine polychaete, constructs a tube-like shelter by cementing together sand grains using a glue secreted from the building organ in its thorax. The glue is a mixture of post-translationally modified proteins, notably the cement proteins Pc-1 and Pc-2 with the amino acid, 3,4-dihydroxyphenyl-L-alanine (DOPA). Significant amounts of a halogenated derivative of DOPA were isolated from the worm cement following partial acid hydrolysis and capture of catecholic amino acids by phenylboronate affinity chromatography. Analysis by tandem mass spectrometry and (1)H NMR indicates the DOPA derivative to be 2-chloro-4, 5-dihydroxyphenyl-L-alanine. The potential roles of 2-chloro-DOPA in chemical defense and underwater adhesion are considered.
authors.affiliation: Molecular, Cell & Developmental Biology Department, University of California at Santa Barbara, Santa Barbara, CA, USA.
authors.forename: Cheng Jun
authors.initials: CJ
authors.lastname: Sun
authors.forename: Aasheesh
authors.initials: A
authors.lastname: Srivastava
authors.forename: Jack R
authors.initials: JR
authors.lastname: Reifert
authors.forename: J Herbert
authors.initials: JH
authors.lastname: Waite