title: Viability and functional capacity after thawing of hematopoietic progenitor cells cryopreserved at a cord blood stem cell bank in Colombia.
abstract: OBJECTIVE
  To evaluate the viability and functional capacity of hematopoietic progenitor cells from cord blood samples cryopreserved at the Banco de Células Stem de Colombia.
  
  
  METHODS
  After thawing and centrifugation of 20 samples, viable white blood cells were numbered by the trypan blue method and CD34(+)CD45(+dim) hematopoietic progenitor cells were numbered by flow cytometry. Clonogenic assays also tested the functional capacity of viable CD34(+)CD45(+dim) cells.
  
  
  RESULTS
  The median rates of viable CD34(+)CD45(+dim) cells were 99.6% before freezing and 73.0% after thawing (P<0.001). The 20 cultures yielded a median of 12 cells with a lineage of red cells, 17.5 cells with a lineage of white cells, and 10 cells with a mixed lineage.
  
  
  CONCLUSIONS
  Although the rate of viable CD34(+)CD45(+dim) cells was decreased by 26.6% after thawing by the method we used, the numbers of CD34(+)CD45(+dim) cells that formed colonies were similar to those obtained by other published methods.
authors.affiliation: Banco de Células Stem de Colombia, Bogotá, Colombia. laboratorio@bancodecelulas.com
authors.forename: Luz Mabel
authors.initials: LM
authors.lastname: Avila Portillo
authors.forename: Juliana
authors.initials: J
authors.lastname: Guzmán
authors.forename: Carolina
authors.initials: C
authors.lastname: Rojas
authors.forename: Andrea
authors.initials: A
authors.lastname: Becerra
authors.forename: Jennifer
authors.initials: J
authors.lastname: Avila
authors.forename: Diego
authors.initials: D
authors.lastname: Jaimes
authors.forename: Benjamín
authors.initials: B
authors.lastname: Ospino
authors.forename: Claudia
authors.initials: C
authors.lastname: Gomez
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Madero
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D002470:Cell Survival; D003105:Colombia; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D011247:Pregnancy
chemical_list: D018952:Antigens, CD34; D017493:Leukocyte Common Antigens