title: Olive oil phenols modulate the expression of metalloproteinase 9 in THP-1 cells by acting on nuclear factor-kappaB signaling.
abstract: In vivo studies suggest that the phenolic component contributes to the anti-inflammatory and antiatherosclerotic actions of olive oil; however, the effects in circulating cells are not fully characterized. Monocytes play a key role in inflammation-based diseases by expressing several molecules, including metalloproteinases (MMPs). In the present study, we investigated the effects of olive oil phenolic extract and individual compounds on MMP-9 in THP-1 cells, a human monocyte-like cell line. Olive oil extract prevented the stimulation of MMP-9 expression and secretion in tumor necrosis factor alpha-treated THP-1 cells. Oleuropein aglycone, a typical olive oil phenol, was active at concentrations found in the extract, although other compounds probably contribute to the biological activity. We also found that the effect of the extract and individual compounds on MMP-9 is due to impaired nuclear factor-kappaB signaling. Our findings provide further evidence on the mechanisms by which olive oil reduces the inflammatory burden associated with disorders, such as atherosclerosis.
authors.affiliation: Research Centre for the Characterization and Safe Use of Natural Compounds-Giovanni Galli, University of Milano, Via Balzaretti, 9, 20133 Milano, Italy. mario.dellagli@unimi.it
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
authors.forename: Rossana
authors.initials: R
authors.lastname: Fagnani
authors.forename: Germana V
authors.initials: GV
authors.lastname: Galli
authors.forename: Omar
authors.initials: O
authors.lastname: Maschi
authors.forename: Federica
authors.initials: F
authors.lastname: Gilardi
authors.forename: Stefano
authors.initials: S
authors.lastname: Bellosta
authors.forename: Maurizio
authors.initials: M
authors.lastname: Crestani
authors.forename: Enrica
authors.initials: E
authors.lastname: Bosisio
authors.forename: Emma
authors.initials: E
authors.lastname: De Fabiani
authors.forename: Donatella
authors.initials: D
authors.lastname: Caruso
mesh_terms: D045744:Cell Line, Tumor; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007951:Leukemia, Myeloid; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D016328:NF-kappa B / Q000235:genetics* / Q000502:physiology; D000069463:Olive Oil; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D016328:NF-kappa B; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D014409:Tumor Necrosis Factor-alpha; D020780:Matrix Metalloproteinase 9