[Separation and purification of total flavonoids in Smilax glabra by polyamide resins].
OBJECTIVE
To investigate the process of separating and purifying total flavonoids from Smilax glabra.


METHODS
With the contents of total flavonoids, astilbin and engelitin as indices, the optimum process of separating and purifying flavonoids from Smilax glabra was screened by static and dynamic adsorption tests.


RESULTS
The static saturated adsorption capacity of polyamide resin to flavonoids of Smilax glabra was 144 mg/g (saturated in 2 h). The dynamic adsorption capacity was 128.125 mg/g, the optimum conditions of elution were as follows: the adsorbed resin was washed by 20BV water firstly, then by 20BV 95% ethanol. The content of obtained total flavonoids reached 71.84%, as the content of astilbin was 14.32%, and the content of engelitin was 1. 08%.


CONCLUSIONS
The total flavonoids of Smilax glabra is able to be easily separated and purified by polyamide resin under the optimum conditions above.
Wu
Xin-Rong
XR
Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China. gzwxrong@yahoo.com
Liu
Zhi-Gang
ZG
Yan
Ren-Liang
RL
Sun
Wei-Feng
WF
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D044948:Flavonols / Q000032:analysis; D009757:Nylons / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D032281:Smilax / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D005419:Flavonoids; D044948:Flavonols; D009757:Nylons; C099069:astilbin