title: The nitric oxide synthase 3 gene polymorphisms and their association with deep vein thrombosis in Asian Indian patients.
abstract: BACKGROUND
  Endothelium derived nitric oxide is formed from l-arginine by endothelial nitric oxide synthase encoded by the nitric oxide synthase 3 (NOS3) gene. Nitric oxide possesses a variety of protective effects on endothelial cells and therefore NOS3 is a logical candidate gene to be investigated for the susceptibility of deep vein thrombosis (DVT).
  
  
  METHODS
  One hundred consecutive patients (M: F=56:44) with idiopathic deep vein thrombosis and an equal number of age and sex matched healthy controls were the study subjects. All study subjects were typed for five NOS3 polymorphisms (-786C/T, -922A/G, 894G/T, Intron 4 VNTR, and Intron 23 G10T).
  
  
  RESULTS
  Two polymorphisms (-922A/G and 894G/T) are showing their association with DVT. -922A/G shows both genotypic (P=0.0218; chi2=5.25; O.R=1.94) as well as allelic association (P=0.0014; chi2=10.19; O.R=2.0) while 894G/T shows only allelic (P=0.04; chi2=3.93; O.R=3.93) association with DVT.
  
  
  CONCLUSIONS
  Susceptibility to DVT in North Indian Asian patients may be associated with some variants of NOS3 gene.
authors.affiliation: All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Mohd Suhail
authors.initials: MS
authors.lastname: Akhter
authors.forename: Arijit
authors.initials: A
authors.lastname: Biswas
authors.forename: Ravi
authors.initials: R
authors.lastname: Ranjan
authors.forename: Amit
authors.initials: A
authors.lastname: Sharma
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Renu
authors.initials: R
authors.lastname: Saxena
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D020246:Venous Thrombosis / Q000235:genetics*; D055815:Young Adult
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III