title: [Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector].
abstract: OBJECTIVE
  To develop a coexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency.
  
  
  METHODS
  The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA.
  
  
  RESULTS
  Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells.
  
  
  CONCLUSIONS
  The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.
authors.affiliation: College of Life sciences, Jilin University, Changchun 130012, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Shen
authors.forename: Su-Jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Shao-Jije
authors.initials: SJ
authors.lastname: Xin
authors.forename: Bing-Ke
authors.initials: BK
authors.lastname: Bai
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Pan-Yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000235:genetics* / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans
chemical_list: D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens