title: Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta).
abstract: Synthesis and accumulation of molecular chaperones are universal responses found in all cellular organisms when exposed to a variety of unfavorable conditions. Heat shock protein 70 (Hsp70), which is one of the major classes of molecular chaperones, plays a particularly important role in cellular stress responses, and the Hsp70 system is the most intensely studied in higher plants and algae. Therefore, we isolated and characterized a cDNA clone encoding Hsp70 from a sterile strain of Ulva pertusa (Ulvales, Chlorophyta). The sterile U. pertusa Hsp70 (UpHsp70) cDNA consisted of 2,272 nucleotides and had an open reading frame encoding a polypeptide of 663 amino acid (AA) residues with a molecular mass of 71.7 kDa. Amino acid alignment and phylogenetic analysis of Hsp70s from other organisms showed that UpHsp70 was more similar to cytoplasmic Hsp70s from green algae and higher plants (> or =75%) than to those from other algae and microorganisms. Southern blot analysis indicated that the sterile U. pertusa genome had at least four cytoplasmic Hsp70-encoding genes. UpHsp70 mRNA levels were significantly affected by diurnal changes, rapidly increased by high-temperature stress, and gradually increased by exposure to copper, cadmium, and lead. These results suggest that UpHsp70 plays particularly important roles in adaptation to high-temperature conditions and diurnal changes, and is potentially involved in tolerance to heavy metal toxicity.
authors.affiliation: Mie University, Tsu, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tominaga
authors.forename: Daniel Adam
authors.initials: DA
authors.lastname: Coury
authors.forename: Hideomi
authors.initials: H
authors.lastname: Amano
authors.forename: Makoto
authors.initials: M
authors.lastname: Kakinuma
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D005298:Fertility; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological; D014158:Transcription, Genetic / Q000187:drug effects; D044445:Ulva / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D019216:Metals, Heavy; D012333:RNA, Messenger