title: Biosensor analysis of blood esterases for organophosphorus compounds exposure assessment: approaches to simultaneous determination of several esterases.
abstract: This paper reviews our previously published data and presents new results on biosensor assay of blood esterases. Tyrosinase and choline oxidase biosensors based on nanostructured polyelectrolyte films were developed for these purposes. Experiments were performed on the quantitative determination of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carboxylesterase (CaE), and neuropathy target esterase (NTE) in samples of whole blood of rats, mice, and humans. Good agreement was found between biosensor and spectrophotometric assays for AChE, BChE, and CaE. No direct comparison could be made for NTE because its activity cannot be measured spectrophotometrically in whole blood. A new method of simultaneous quantitative determination of AChE and BChE in test mixtures is also described. This method represents a bifunctional biosensor for the simultaneous analysis of choline and phenol based on integration of individual sensors. Algorithms for calculation of separate concentrations of AChE and BChE in the mixture were developed. The mean error of calculated component concentrations was approximately 6% for binary test mixtures. The present work provides a foundation for building multiplexed systems for the simultaneous determination of multiple esterases with applications to biomonitoring for exposures to organophosphorus compounds.
authors.affiliation: Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Sigolaeva
authors.forename: Galina
authors.initials: G
authors.lastname: Makhaeva
authors.forename: Elena
authors.initials: E
authors.lastname: Rudakova
authors.forename: Natalia
authors.initials: N
authors.lastname: Boltneva
authors.forename: Marya
authors.initials: M
authors.lastname: Porus
authors.forename: Galina
authors.initials: G
authors.lastname: Dubacheva
authors.forename: Arkadi
authors.initials: A
authors.lastname: Eremenko
authors.forename: Ilya
authors.initials: I
authors.lastname: Kurochkin
authors.forename: Rudy J
authors.initials: RJ
authors.lastname: Richardson
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D002417:Cattle; D002619:Chemical Warfare Agents / Q000633:toxicity; D004950:Esterases / Q000097:blood* / Q000378:metabolism; D008297:Male; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000633:toxicity*; D051381:Rats; D013997:Time Factors
chemical_list: D002619:Chemical Warfare Agents; D009943:Organophosphorus Compounds; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D014442:Monophenol Monooxygenase; D004950:Esterases