Ultraendurance exercise increases the production of reactive oxygen species in isolated mitochondria from human skeletal muscle.
Exercise-induced oxidative stress is important for the muscular adaptation to training but may also cause muscle damage. We hypothesized that prolonged exercise would increase mitochondrial production of reactive oxygen species (ROS) measured in vitro and that this correlates with oxidative damage. Eight male athletes (24-32 yr) performed ultraendurance exercise (kayaking/running/cycling) with an average work intensity of 55% V(O(2peak)) for 24 h. Muscle biopsies were taken from vastus lateralis before exercise, immediately after exercise, and after 28 h of recovery. The production of H(2)O(2) was measured fluorometrically in isolated mitochondria with the Amplex red and peroxidase system. Succinate-supported mitochondrial H(2)O(2) production was significantly increased after exercise (73% higher, P = 0.025) but restored to the initial level at recovery. Plasma level of free fatty acids (FFA) increased fourfold and exceeded 1.2 mmol/l during the last 6 h of exercise. Plasma FFA at the end of exercise was significantly correlated to mitochondrial ROS production (r = 0.74, P < 0.05). Mitochondrial content of 4-hydroxy-nonenal-adducts (a marker of oxidative damage) was increased only after recovery and was not correlated with mitochondrial ROS production. Total thiol group level and glutathione peroxidase activity were elevated after recovery. In conclusion, ultraendurance exercise increases ROS production in isolated mitochondria, but this is reversed after 28 h recovery. Mitochondrial ROS production was not correlated with oxidative damage of mitochondrial proteins, which was increased at recovery but not immediately after exercise.
Sahlin
Kent
K
The Swedish School of Sport and Health Sciences (GIH), Astrand Laboratory of Work Physiology, Stockholm, Sweden. kent.sahlin@gih.se
Shabalina
Irina G
IG
Mattsson
C Mikael
CM
Bakkman
Linda
L
Fernström
Maria
M
Rozhdestvenskaya
Zinaida
Z
Enqvist
Jonas K
JK
Nedergaard
Jan
J
Ekblom
Björn
B
Tonkonogi
Michail
M
D000328:Adult; D056352:Athletes; D001706:Biopsy; D003402:Creatine Kinase / Q000378:metabolism; D015444:Exercise; D017079:Exercise Tolerance / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D052097:Quadriceps Muscle / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055815:Young Adult
D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D003402:Creatine Kinase