title: Aquaporin-6 expression in the cochlear sensory epithelium is downregulated by salicylates.
abstract: We characterize the expression pattern of aquaporin-6 in the mouse inner ear by RT-PCR and immunohistochemistry. Our data show that in the inner ear aquaporin-6 is expressed, in both vestibular and acoustic sensory epithelia, by the supporting cells directly contacting hair cells. In particular, in the Organ of Corti, expression was strongest in Deiters' cells, which provide both a mechanical link between outer hair cells (OHCs) and the Organ of Corti, and an entry point for ion recycle pathways. Since aquaporin-6 is permeable to both water and anions, these results suggest its possible involvement in regulating OHC motility, directly through modulation of water and chloride flow or by changing mechanical compliance in Deiters' cells. In further support of this role, treating mice with salicylates, which impair OHC electromotility, dramatically reduced aquaporin-6 expression in the inner ear epithelia but not in control tissues, suggesting a role for this protein in modulating OHCs' responses.
authors.affiliation: Department of Physiology, Section of General Physiology, University of Pavia, 27100 Pavia, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Perin
authors.forename: Simona
authors.initials: S
authors.lastname: Tritto
authors.forename: Laura
authors.initials: L
authors.lastname: Botta
authors.forename: Jacopo Maria
authors.initials: JM
authors.lastname: Fontana
authors.forename: Giulia
authors.initials: G
authors.lastname: Gastaldi
authors.forename: Sergio
authors.initials: S
authors.lastname: Masetto
authors.forename: Marisa
authors.initials: M
authors.lastname: Tosco
authors.forename: Umberto
authors.initials: U
authors.lastname: Laforenza
mesh_terms: D000818:Animals; D051403:Aquaporin 6 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology*; D003051:Cochlea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D015536:Down-Regulation / Q000187:drug effects; D004848:Epithelium / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009925:Organ of Corti / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D051403:Aquaporin 6; D012333:RNA, Messenger; D001241:Aspirin