title: Immunophenotypic, cytogenetic and clinical features of 113 acute lymphoblastic leukaemia patients in China.
abstract: BACKGROUND
  The analysis of immunophenotype of the leukaemic cells has been of great importance for the diagnosis, classification and prognosis of acute lymphoblastic leukaemia (ALL).
  
  
  METHODS
  One hundred and thirteen Chinese patients with ALL were immunophenotyped by fl ow cytometry and 74 cases were also subjected to karyotype analysis by G-banding technology.
  
  
  RESULTS
  Of the 113 Chinese ALL patients, 14.2% were identified as T-ALL and 85.8% as B-ALL. Myeloid antigen (MyAg) expression was documented in 34.9% of the cases analysed and CD13 was most commonly expressed MyAg in ALL patients (23.6%). MyAg positivity was higher in adult with ALL (47.6%) than in children with ALL (26.6%). Abnormal karyotypes were detected in 39 out of 74 (52.7%) cases. The clinical and biological characteristics of ALL patients between MyAg+ and MyAg- groups showed that increased white blood count (WBC) (>50 x 109/L), higher CD34 positivity and higher percentage of adult patients were found to be correlated with MyAg+ ALL.
  
  
  CONCLUSIONS
  Our results indicate that the immunophenotype did have relevance to the abnormal cytogenetic changes and clinical features in ALL. Flow cytometry immunophenotype has become the most important method for diagnosis and typing of ALL.
authors.affiliation: Laboratory of Hematology, Shengjing Hospital of China Medical University, China. tonghaixia18@yahoo.com.cn
authors.forename: Haixia
authors.initials: H
authors.lastname: Tong
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Chunwei
authors.initials: C
authors.lastname: Lu
authors.forename: Zhuogang
authors.initials: Z
authors.lastname: Liu
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D020732:Cytogenetic Analysis; D004171:Diploidy; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D008875:Middle Aged; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification* / Q000175:diagnosis / Q000235:genetics*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification* / Q000175:diagnosis / Q000235:genetics*; D014178:Translocation, Genetic; D055815:Young Adult