title: Novel colorimetric sensor for oral malodour.
abstract: Volatile sulphur compounds are the primary constituents of oral malodour. Quantitative tools for the detection of oral malodour are beneficial to evaluate the intensity of malodour, analyse its causes and monitor the effectiveness of customized treatments. We have developed an objective, cost effective, do-it-yourself colorimetric sensor for oral malodour quantification. The sensor consisted of a sensing solution, a gas sampling unit for collecting a known volume of mouth air and a photometric detector. The sensing solution was iodine and the depletion of iodine on reaction with hydrogen sulphide was detected colorimetrically using starch. The detection limit of the sensor is 0.05 microg L(-1) of hydrogen sulphide, which is fit-for-purpose for oral malodour detection in healthy subjects as well as halitosis patients. Volatile sulphur compounds in mouth air were quantified in healthy human volunteers using this portable sensor and the detected levels were in the range of 0.2-0.4 microg L(-1). There was a good correlation between the VSC levels detected by the colorimetric sensor and halimeter (R(2)=0.934). The developed sensor can be easily fabricated in the laboratory, and it shows high potential to be used as a clinical evaluation tool for oral malodour assessments.
authors.affiliation: Hindustan Unilever Research Center, 64 Main Road, Whitefield, Bangalore 560066, India.
authors.forename: Nethaji
authors.initials: N
authors.lastname: Alagirisamy
authors.forename: Sarita S
authors.initials: SS
authors.lastname: Hardas
authors.forename: Sujatha
authors.initials: S
authors.lastname: Jayaraman
mesh_terms: D001944:Breath Tests / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation*; D006209:Halitosis / Q000175:diagnosis*; D006801:Humans; D007455:Iodine / Q000737:chemistry; D010084:Oxidation-Reduction; D010783:Photometry; D013457:Sulfur Compounds / Q000032:analysis / Q000737:chemistry*
chemical_list: D013457:Sulfur Compounds; D007455:Iodine