[The contents changes of polysaccharides and total flavonoids during flower bud differentiation of Epimedium sagittatum].
OBJECTIVE
To study the dynamic changes of the contents of polysaccharides and total flavonoids Daring flower bud differentiation of Epimedium sagittatum.


METHODS
The polysaccharides and total flavonoids from Epimedium sagittatum were extracted by Microwave-assisted method. The content of polysaccharides was determined by anthrone sulphuric acid colorimetry and that of total flavonoids was determined at 270 nm by ultraviolet spectrophotometry.


RESULTS
The polysaccharides content in leaf decreased during the initial stages of floral bud differentiation and then increased, while the content in stem and fibrous root decreased steadily until floral bud differentiation completed. The content in rhizome kept invariant. In the flower bud physiological differentiation period,the content of total flavonoids in leaf and stem decreased and then kept invariant, while the content in rhizome increased and kept invariant. The content of total flavonoids in fibrous root increased during the initial stages of floral bud differentiation and then decreased.


CONCLUSIONS
The contents of polysaccharide and total flavonoids have relation with the flower bud differentiation of Epimedium sagittatum.
Wang
Rong
R
College of Life Science, China West Normal University, Nanchong 637002, China. 0532wangrong@163.com
Li
Yun-Xiang
YX
Quan
Qiu-Mei
QM
D034181:Epimedium / Q000254:growth & development* / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism*; D035264:Flowers / Q000254:growth & development*; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
D005419:Flavonoids; D011134:Polysaccharides