Loss of ability to self-heal malaria upon taurine transporter deletion.
Deletion of the taurine transporter gene (taut) results in lowered levels of taurine, the most abundant amino acid in mammals. Here, we show that taut-/- mice have lost their ability to self-heal blood-stage infections with Plasmodium chabaudi malaria. All taut-/- mice succumb to infections during crisis, while about 90% of the control taut(+/+) mice survive. The latter retain unchanged taurine levels even at peak parasitemia. Deletion of taut, however, results in the lowering of circulating taurine levels from 540 to 264 micromol/liter, and infections cause additional lowering to 192 micromol/liter. Peak parasitemia levels in taut-/- mice are approximately 60% higher than those in taut(+/+) mice, an elevation that is associated with increased systemic tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) levels, as well as with liver injuries. The latter manifest as increased systemic ammonia levels, a perturbed capacity to entrap injected particles, and increased expression of genes encoding TNF-alpha, IL-1beta, IL-6, inducible nitric oxide synthase (iNOS), NF-kappaB, and vitamin D receptor (VDR). Autopsy reveals multiorgan failure as the cause of death for malaria-infected taut-/- mice. Our data indicate that taut-controlled taurine homeostasis is essential for resistance to P. chabaudi malaria. Taurine deficiency due to taut deletion, however, impairs the eryptosis of P. chabaudi-parasitized erythrocytes and expedites increases in systemic TNF-alpha, IL-1beta, and ammonia levels, presumably contributing to multiorgan failure in P. chabaudi-infected taut-/- mice.
Delic
Denis
D
Division of Molecular Parasitology, Department of Biology, and Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine- University, Universitätsstr 1, Clinic for Gastroenterology, Hepatology, and Infectiology, Heinrich-Heine-University, Moorenstr 5, 40225 Düsseldorf, Germany.
Warskulat
Ulrich
U
Borsch
Elena
E
Al-Qahtani
Saad
S
Al-Quraishi
Saleh
S
Häussinger
Dieter
D
Wunderlich
Frank
F
D000641:Ammonia / Q000097:blood; D000818:Animals; D005260:Female; D053583:Interleukin-1beta / Q000097:blood; D008099:Liver / Q000473:pathology; D008288:Malaria / Q000276:immunology* / Q000401:mortality* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000172:deficiency*; D026901:Membrane Transport Proteins / Q000172:deficiency*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009102:Multiple Organ Failure; D018512:Parasitemia; D016788:Plasmodium chabaudi / Q000276:immunology* / Q000472:pathogenicity*; D017384:Sequence Deletion; D016019:Survival Analysis; D013654:Taurine / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D000099247:Taurine Transporters
D000641:Ammonia; D053583:Interleukin-1beta; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D013654:Taurine; D014409:Tumor Necrosis Factor-alpha; D000099247:Taurine Transporters