title: Antioxidant and cytotoxic activities of Aphanes arvensis extracts.
abstract: The objectives of this study were to examine the free radical scavenging activity and the protective effects against macromolecular oxidation as well as the cytotoxic activity of Aphanes arvensis aqueous and methanolic extracts. Free radical scavenging activity was determined by DPPH method. The methanolic extract showed a scavenging activity nearly equivalent to Trolox and Vitamin C and has an IC(50) value of 4.54 microg/mL. Total antioxidant capacity was determined by CUPRAC method. The antioxidant capacity of aqueous and methanolic extract was 0.792 and 1.550 mmol TE/g DWE, respectively. The protective effect of A. arvensis extracts against lipid peroxidation was evaluated using a liposome oxidation system. The methanolic extract was more active than the aqueous extract. The aqueous extract possessed protective effect against protein oxidation in a dose dependent manner. Both extracts showed inhibitory effect on DNA oxidation as measured by plasmid relaxation assay. Results presented here indicate that A. arvensis possess strong antioxidant activity and protective effects with very little cytotoxic effect, and they can therefore be used as a natural additive in food, cosmetic and pharmaceutical industries.
authors.affiliation: Biochemistry Department, Upper Nile University, Khartoum, Sudan.
authors.forename: Ismail
authors.initials: I
authors.lastname: Hamad
authors.forename: Ozlem
authors.initials: O
authors.lastname: Erol-Dayi
authors.forename: Murat
authors.initials: M
authors.lastname: Pekmez
authors.forename: Evren
authors.initials: E
authors.lastname: Onay-Uçar
authors.forename: Nazli
authors.initials: N
authors.lastname: Arda
mesh_terms: D000975:Antioxidants / Q000009:adverse effects / Q000494:pharmacology*; D001205:Ascorbic Acid; D002839:Chromans; D003603:Cytotoxins; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects*; D027824:Rosaceae
chemical_list: D000975:Antioxidants; D002839:Chromans; D003603:Cytotoxins; D016166:Free Radical Scavengers; D008081:Liposomes; D010936:Plant Extracts; D004247:DNA; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid