Gene expression profiling in rat pancreas after ventromedial hypothalamic lesioning.
OBJECTIVE
We previously reported that vagal hyperactivity produced by ventromedial hypothalamic (VMH) lesions stimulated cell proliferation of rat pancreatic islet B and acinar cells primarily through a cholinergic receptor mechanism.


METHODS
This study examined how gene families involved in cell proliferation are regulated after VMH lesions formation. Total pancreatic RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH-lesioned rats were investigated using DNA microarray and real-time polymerase chain reaction.


RESULTS
The VMH lesions regulated the genes that are involved in functions related to cellular growth and proliferation and neuronal development in the pancreas. Real-time polymerase chain reaction also confirmed that gene expressions of angiotensin II receptor-like 1 (AGTRL1) and proline rich 15 (PRR15) were down-regulated at day 3 after the VMH lesions.


CONCLUSIONS
Ventromedial hypothalamic lesions may change the expression of cell proliferation-related genes and neuron-related genes in a rat pancreas.
Kiba
Takayoshi
T
Faculty of Health Care, Kiryu University, Midori, Gunma, Japan.
Kintaka
Yuri
Y
Suzuki
Yoko
Y
Ishizuka
Noriko
N
Ishigaki
Yasuhito
Y
Inoue
Shuji
S
D000818:Animals; D000075244:Apelin Receptors; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D010179:Pancreas / Q000294:innervation* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D014697:Ventromedial Hypothalamic Nucleus / Q000293:injuries* / Q000502:physiology
D000075244:Apelin Receptors; C491284:Aplnr protein, rat; D043562:Receptors, G-Protein-Coupled