title: Supplementation of fat grafts with adipose-derived regenerative cells improves long-term graft retention.
abstract: Current practice of autologous fat transfer for soft tissue augmentation is limited by poor long-term graft retention. Adipose-derived regenerative cells (ADRCs) contain several types of stem and regenerative cells, which may help improve graft retention through multiple mechanisms. Using a murine fat transplantation model, ADRCs were added to transplanted fat to test whether ADRCs could improve the long-term retention of the grafts. This study showed, at both 6 and 9 months after transplantation, ADRCs not only increased graft retention by 2-fold but also improved the quality of the grafts. ADRC-supplemented grafts had a higher capillary density, indicating ADRCs could promote neovascularization. Further cell tracking and gene expression studies suggest ADRCs may promote angiogenesis and adipocyte differentiation and prevent apoptosis through the expression of various growth factors, including VEGFA and IGF-1. Taken together, these results suggest a potential clinical utility of ADRCs in facilitating autologous fat transfer for soft tissue augmentation.
authors.affiliation: Department of Regenerative Cell Technology, Cytori Therapeutics, Inc., 3020 Callan Road, San Diego, CA 92121, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Ronda
authors.initials: R
authors.lastname: Schreiber
authors.forename: John T
authors.initials: JT
authors.lastname: Ransom
authors.forename: John K
authors.initials: JK
authors.lastname: Fraser
authors.forename: Marc H
authors.initials: MH
authors.lastname: Hedrick
authors.forename: Kai
authors.initials: K
authors.lastname: Pinkernell
authors.forename: Hai-Chien
authors.initials: HC
authors.lastname: Kuo
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation; D005260:Female; D007150:Immunohistochemistry; D007511:Ischemia / Q000503:physiopathology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012038:Regeneration / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*