Comparison of paired cervical scrape and tumor tissue samples for detection of human papillomaviruses in patients with cervical cancer.
OBJECTIVE
To compare the detection and distribution of HPV genotypes in paired cervical scrape samples and tumor tissue samples in patients with cervical cancer.


METHODS
Forty cervical scrape samples and 40 paired archival or fresh frozen tissue samples were collected from women with cervical cancer. Polymerase chain reaction with GP5+ and GP6+ primers was performed in all samples for HPV DNA detection. All GP5+/GP6+ negative samples were additionally tested using INNO-LiPA HPV Genotyping Extra Test.


RESULTS
Overall, 39/40 (97.5%) of CC samples were HPV DNA positive. HPV 16 was found in 24/40 samples, HPV 18 in 5/40 samples. A co-infection with two different HPV genotypes was identified in one cervical scrape specimen, while in tissue samples only single infections were detected. Overall agreement between paired samples was 98.75%.


CONCLUSIONS
The present study has shown that cervical scrape samples are equally useful for HPV genotype determination as tumor tissue samples in patients with cervical cancer. They can be used as accurate clinical samples for detection of HPV genotype causing cervical cancer or for epidemiological molecular studies.
Jancar
N
N
Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Kocjan
B J
BJ
Poljak
M
M
Bokal
E Vrtacnik
EV
D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics* / Q000302:isolation & purification; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult