Characterization of the antigenic O-polysaccharide produced by Escherichia coli serotype O:70.
The structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of D-glucose, D-galactose, D-fucose, 2-acetamido-2-deoxy-D-galactose, and 3-acetamido-3-deoxy-D-quinovose in a ratio of 1:1:1:1:1. From the use of DOC-PAGE, methylation, Smith-type periodate oxidation, and (1)H and (13)C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structure: [structure: see the text]
MacLean
Leann L
LL
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6.
Perry
Malcolm B
MB
D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000276:immunology*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology*
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate