Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma.
In the cytosol of lipogenic tissue, ketone bodies are activated by AACS (acetoacetyl-CoA synthetase) and incorporated into cholesterol and fatty acids. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. In order to elucidate the mechanism controlling the gene expression of human AACS and to clarify its physiological role, we isolated the human promoter, characterized the elements required to initiate transcription and analysed the expression of the gene in response to PPARgamma (peroxisome-proliferator-activated receptor gamma), an inducer of adipogenesis. We show that the human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1).
Aguiló
Francesca
F
Department of Biochemistry and Molecular Biology, Institute of Biomedicine of Barcelona University, Spain.
Camarero
Nuria
N
Relat
Joana
J
Marrero
Pedro F
PF
Haro
Diego
D
D050156:Adipogenesis; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D047495:PPAR gamma / Q000502:physiology*; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic
D047495:PPAR gamma; D016329:Sp1 Transcription Factor; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase