Interaction of Sedlin with PAM14.
Sedlin is an evolutionarily conserved and ubiquitously expressed protein that is encoded by the gene SEDL. Mutations in the latter are known to be causative for spondyloepiphyseal dysplasia tarda. However, the mechanism underlying this remains unclear. We have previously shown that Sedlin interacts with the intracellular chloride channel proteins CLIC1 and CLIC2 in the cytoplasm. In this report we show that Sedlin is also physically associated with protein associated with MRG 14 kDa (PAM14), a nuclear protein that interacts with the transcription factor MORF4-related gene on chromosome 15 (MRG15). This was suggested by yeast two-hybrid screening and was confirmed with GST pull-down and immunoprecipitation assays. Moreover, we demonstrate that the C-terminus of Sedlin and the N-terminus of PAM14 are critical for their interaction. Together, these results suggest that nucleus-localized Sedlin may play a role in regulation of transcriptional activities of the MRG family of transcription factors via binding to PAM14.
Liu
Xiaoying
X
Institute of Clinical Pharmacology, Anhui Medical University, 81 Meishan Rd., Hefei, Anhui 230032, People's Republic of China.
Wang
Yunfei
Y
Zhu
Hengrui
H
Zhang
Qinghui
Q
Xing
Xin
X
Wu
Biao
B
Song
Lihua
L
Fan
Libin
L
D000818:Animals; D019556:COS Cells; D002466:Cell Nucleolus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D047908:Intracellular Signaling Peptides and Proteins; C439067:MRFAP1 protein, human; D026901:Membrane Transport Proteins; D009687:Nuclear Proteins; C120759:TRAPPC2 protein, human; D014157:Transcription Factors