title: Functional properties of the human ventral mesencephalic neural stem cell line hVM1.
abstract: The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiological properties of functional neurons and respond to neurotransmitters in vitro. However, the electrophysiological properties are immature and the cells require longer maturation time than possible under in vitro conditions.
authors.affiliation: Experimental Epilepsy Group, Wallenberg Neuroscience Center, Lund University Hospital, 221 84, Lund, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Tønnesen
authors.forename: Emma Gonzalez
authors.initials: EG
authors.lastname: Seiz
authors.forename: Milagros
authors.initials: M
authors.lastname: Ramos
authors.forename: Olle
authors.initials: O
authors.lastname: Lindvall
authors.forename: Alberto
authors.initials: A
authors.lastname: Martinez-Serrano
authors.forename: Merab
authors.initials: M
authors.lastname: Kokaia
mesh_terms: D000200:Action Potentials / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology*; D005333:Fetus / Q000166:cytology; D006801:Humans; D008636:Mesencephalon / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D018408:Patch-Clamp Techniques; D033581:Stem Cell Transplantation; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D003470:Culture Media; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine