Expression changes of serotonin receptor gene subtype 5HT3a in peripheral blood mononuclear cells from schizophrenic patients treated with haloperidol and Olanzapin.
Serotonin receptors are involved in pathophysiology of schizophrenia and may mediate other neurotransmitter effects. We investigated serotonin receptors gene expression in peripheral blood mononuclear cells (PBMC) of naïve schizophrenic patients, before and after treatment. Also serotonin receptor gene expression was compared in two treatment groups including Haloperidol and Olanzapine. The PBMC was separated from whole blood by Ficoll-hypaque. The total cellular RNA was extracted and the cDNA was synthesized. This process was followed by real-time PCR using primer pairs specific for 5HT(3a) serotonin receptor mRNA and beta-actin as internal control. The results showed the presence of subtype of serotonin receptor in lymphocytes. Serotonin gene expression showed significant changes in Olanzapine treatment group which correlated with Clinical Global Impression (CGI) score improvement. In conclusion, the present study has shown that human PBMC express serotonin receptors 5HT(3a). Moreover, clinical symptom improvement of Olanzapin may be demonstrated by a change in serotonin receptor gene expression.
Shariati
Gholam Reza
GR
Department of Medical Genetic, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. ghah@nrcgeb.ac.ir.
Ahangari
Ghasem
G
Hossein-nezhad
Arash
A
Asadi
Seyed Mohammad
SM
Pooyafard
Farzaneh
F
Ahmadkhaniha
Hamid Reza
HR
D000328:Adult; D014150:Antipsychotic Agents / Q000627:therapeutic use*; D001569:Benzodiazepines / Q000627:therapeutic use*; D006220:Haloperidol / Q000627:therapeutic use*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D000077152:Olanzapine; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics*; D012559:Schizophrenia / Q000188:drug therapy* / Q000378:metabolism
D014150:Antipsychotic Agents; D044406:Receptors, Serotonin, 5-HT3; D001569:Benzodiazepines; D006220:Haloperidol; D000077152:Olanzapine