Bovine papillomavirus type 1 E2 and E7 proteins down-regulate Toll Like Receptor 4 (TLR4) expression in equine fibroblasts.
BPV-1 and less commonly BPV-2 are associated with the pathogenesis of equine skin tumours termed sarcoids. We recently documented the transcriptional changes that are induced by BPV-1 in equine fibroblasts using microarray analyses. TLR4 expression was found to be significantly down-regulated by BPV-1. In the present study, we show that TLR4 expression is significantly decreased following the exogenous expression of BPV-1 E2 and E7 in primary equine fibroblasts. The results were confirmed by the demonstration of increased TLR4 expression following siRNA suppression of BPV-1 E2 and E7 viral gene expression. These data imply that BPV-1 is able to subvert the innate immune response by downregulation of TLR4.
Yuan
Z Q
ZQ
Division of Pathological Sciences, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Garscube Campus, Glasgow, Scotland G61 1QH, UK.
Bennett
L
L
Campo
M S
MS
Nasir
L
L
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000276:immunology* / Q000472:pathogenicity; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000276:immunology*; D015536:Down-Regulation; D018397:Equidae; D005347:Fibroblasts / Q000821:virology*; D020868:Gene Silencing; D054884:Host-Pathogen Interactions; D009856:Oncogene Proteins, Viral / Q000037:antagonists & inhibitors / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis*; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000276:immunology*
D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D009856:Oncogene Proteins, Viral; D034741:RNA, Small Interfering; D051197:Toll-Like Receptor 4; D014764:Viral Proteins; C075621:oncogene protein E7, Bovine papillomavirus type 1