title: Novel double-antigen sandwich immunoassay for human hepatitis B core antibody.
abstract: A novel diagnostic immunoassay testing procedure for hepatitis B virus core antibody (anti-HBc) using homogeneous purified full-length hepatitis B virus core antigen (HBcAg) capsids obtained from Escherichia coli was compared with Abbott Architect anti-HBc chemiluminescent microparticle immunoassay (CMIA; indirect method) against a library of specimens. A monoclonal anti-HBc neutralization confirmatory assay was then used to determine the degree of discordance between specimens. The new assay was found to be superior in both sensitivity and specificity.
authors.affiliation: National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science, Xiamen University, Xiamen, Fujian Province 361005, People's Republic of China.
authors.forename: An
authors.initials: A
authors.lastname: Li
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Zhiyin
authors.initials: Z
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.forename: Ruyi
authors.initials: R
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Lou
authors.forename: Qin
authors.initials: Q
authors.lastname: Zheng
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Ge
authors.forename: Yixin
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Su
authors.forename: Anthony E T
authors.initials: AE
authors.lastname: Yeo
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000175:diagnosis* / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000097:blood*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens