Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytes.
Understanding mechanisms of post-transcriptional control of gene expression has come under much scrutiny in recent years. A key question in this field is how the translation of specific mRNAs is activated or repressed both spatially and temporally in a given cell. In oocytes of the frog Xenopus laevis a number of mRNAs are localized early in oogenesis and subsequently translated at later stages. We have developed a highly active cell-free translation system from oocytes in the early stages of oogenesis that is applicable to the study of translation and translational control of both endogenous and exogenous mRNAs.
Pratt
Catherine A
CA
Department of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI 02912, USA.
Mowry
Kimberly L
KL
D000818:Animals; D002474:Cell-Free System; D003584:Cytological Techniques; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D008957:Models, Genetic; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014982:Xenopus laevis / Q000378:metabolism*
D012333:RNA, Messenger; D008156:Luciferases