title: Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus.
abstract: Brucella abortus is the intracellular bacterium that causes bovine brucellosis and a chronic human disease known as undulant fever. Interferon (IFN)-gamma plays critical roles in defending against intracellular bacterial infection. In this experiment, we demonstrated the difference in IFN-gamma production between the splenocytes of mice inoculated with outer membrane proteins (OMPs) of B. abortus and whole live bacteria. Our results showed that the OMP-inoculated group showed more IFN-gamma production than did the bacteria-infected group, suggesting that OMPs are candidates for the induction of immune response.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Cha
authors.forename: Nabin
authors.initials: N
authors.lastname: Rayamajhi
authors.forename: Mi-Lan
authors.initials: ML
authors.lastname: Kang
authors.forename: Won-Jung
authors.initials: WJ
authors.lastname: Lee
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Han-Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit; D007371:Interferon-gamma