KIF5C, a kinesin motor involved in apical trafficking of MDCK cells.
Polarized traffic in epithelial cells depends on well-organized pathways that direct secretory cargo to the apical or basolateral plasma membrane. In MDCK cells, apical trafficking can be further divided into a lipid raft-dependent and a raft-independent route, which separate biosynthetic cargo in a post-Golgi endosomal compartment. We have now identified KIF5C as a kinesin motor for apical trafficking of both raft-associated sucrase isomaltase and raft-independent neurotrophin receptor. KIF5C was identified by mass spectrometry in vesicle enriched fractions and on immunoisolated post-Golgi vesicles carrying apical cargo. The amount of vesicle-associated KIF5C was highest on material isolated directly after trans-Golgi network release and declined thereafter. Altogether, our data suggest that KIF5C is involved in the passage of apical cargo molecules to a post-Golgi endosomal compartment, where further segregation into distinct vesicle populations proceeds.
Astanina
Ksenia
K
Department of Cell Biology and Cell Pathology, Philipps University of Marburg, Marburg, Germany.
Jacob
Ralf
R
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism; D015151:Immunoblotting; D047468:Immunoprecipitation; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D016547:Kinesins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000494:pharmacology; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
D034741:RNA, Small Interfering; D020800:Receptor, Nerve Growth Factor; D016547:Kinesins