Identification of putative innate immune related genes from a cell line of the mosquito Aedes albopictus following bacterial challenge.
We report identification of putative innate immune related genes from a cell line of the mosquito Aedes albopictus challenged with heat-killed bacteria. Using a subtractive hybridization and sequencing approach, we analyzed a total 309 expressed sequence tags (ESTs) which clustered in 40 contigs. Thirty-five percent of genes yielded homology to known immune genes corresponding to antimicrobial peptides (AMPs), pathogen-associated molecular patterns, protease and immune signaling cascades. Interestingly, most of the genes have not been previously described from this mosquito and thus represent a class of novel immune genes. Further, 25% sequences did not match to any known species in the non-redundant databases, appear to be specific to the mosquito A. albopictus and merit further study.
Dixit
Rajnikant
R
Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune, India. dixit2k@yahoo.com
Patole
Millind S
MS
Shouche
Yogesh S
YS
D000330:Aedes / Q000166:cytology / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D016000:Cluster Analysis; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D023082:Defensins / Q000235:genetics; D005340:Fibrinogen / Q000235:genetics; D020869:Gene Expression Profiling; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D051192:Receptors, Pattern Recognition / Q000235:genetics; D016415:Sequence Alignment; D057057:Serine Proteases / Q000235:genetics; D015843:Serpins / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D000511:alpha-Macroglobulins / Q000235:genetics
D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D018076:DNA, Complementary; D023082:Defensins; D019476:Insect Proteins; D012333:RNA, Messenger; D051192:Receptors, Pattern Recognition; D015843:Serpins; D000511:alpha-Macroglobulins; C118897:peptidoglycan recognition protein; D005340:Fibrinogen; D057057:Serine Proteases