title: Bacillus oceanisediminis sp. nov., isolated from marine sediment.
abstract: A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample from the South Sea in China. The isolate, designated H2(T), grew at 4-45 °C (optimum 37 °C) and pH 6-10 (optimum pH 7.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major isoprenoid quinone was MK-7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. The major fatty acid was iso-C(15 : 0). The genomic DNA G+C content of strain H2(T) was 44.8mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a monophyletic clade with Bacillus firmus IAM 12464(T). DNA-DNA relatedness between the isolate and B. firmus ATCC 14575(T) was low (27.5 %). Strain H2(T) also had a phenotypic profile that readily distinguished it from its closest phylogenetic neighbours. It is evident from the combination of genotypic and phenotypic data that the organism should be classified in a novel species of the genus Bacillus, for which the name Bacillus oceanisediminis sp. nov. is proposed. The type strain is H2(T) (=CGMCC 1.10115(T) =JCM 16506(T)).
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Jiewei
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Caiyuan
authors.initials: C
authors.lastname: Fang
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xin
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qu
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Ding
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009792:Oceans and Seas; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7