Functional properties of the human ventral mesencephalic neural stem cell line hVM1.
The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiological properties of functional neurons and respond to neurotransmitters in vitro. However, the electrophysiological properties are immature and the cells require longer maturation time than possible under in vitro conditions.
Tønnesen
Jan
J
Experimental Epilepsy Group, Wallenberg Neuroscience Center, Lund University Hospital, 221 84, Lund, Sweden.
Seiz
Emma Gonzalez
EG
Ramos
Milagros
M
Lindvall
Olle
O
Martinez-Serrano
Alberto
A
Kokaia
Merab
M
D000200:Action Potentials / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology*; D005333:Fetus / Q000166:cytology; D006801:Humans; D008636:Mesencephalon / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D018408:Patch-Clamp Techniques; D033581:Stem Cell Transplantation; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D003470:Culture Media; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine