[Fluorescent microbeads-based multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever].
OBJECTIVE
To develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.


METHODS
The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits.


RESULTS
For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously.


CONCLUSIONS
A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.
Li
Jian-dong
JD
State Key Laboratory for Infectious Diseases, Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Shuo
S
Zhang
Quan-fu
QF
Liu
Qin-zhi
QZ
Wei
Yan
Y
Li
Chuan
C
Liang
Mi-fang
MF
Li
De-xin
DX
D000914:Antibodies, Viral / Q000097:blood*; D005453:Fluorescence; D006482:Hemorrhagic Fevers, Viral / Q000097:blood* / Q000276:immunology / Q000821:virology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007075:Immunoglobulin M / Q000097:blood*; D008863:Microspheres; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000276:immunology; D014780:Viruses / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D019590:Nucleocapsid Proteins