Human progenitor-derived endothelial cells vs. venous endothelial cells for vascular tissue engineering: an in vitro study.
The isolation of endothelial progenitor cells from human peripheral blood generates a great hope in vascular tissue engineering because of particular benefit when compared with mature endothelial cells. We explored the capability of progenitor-derived endothelial cells (PDECs) to line fibrin and collagen scaffolds in comparison with human saphenous and umbilical cord vein endothelial cells (HSVECs and HUVECs): (a) in a static situation, allowing definition of the optimal cell culture conditions with different media and cell-seeding densities to check cell behaviour; (b) under shear stress conditions (flow chambers or tubular vascular constructs), allowing investigation of cell response and mRNA expression on both substrates by oligonucleotide microarray analysis and quantitative real-time PCR. Well characterized PDECs: (a) could not be expanded adequately with the usual mature ECs culture media; (b) were able to colonize and grow on fibrin glue; (c) exhibited higher resistance to oxidative stress than HSVECs and HUVECs; (d) withstood physiological shear stress when lining both substrates in flow chambers, and their gene expression was regulated; (e) colonized a collagen-impregnated vascular prosthesis and were able to sense mechanical forces. Our results provide an improved qualification of PDECs for vascular tissue engineering.
Thebaud
Noélie B
NB
INSERM, U577 Bordeaux, Université Victor Segalen Bordeaux 2, Bordeaux, F-33076 France.
Bareille
Reine
R
Remy
Murielle
M
Bourget
Chantal
C
Daculsi
Richard
R
Bordenave
Laurence
L
D001807:Blood Vessel Prosthesis; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D003470:Culture Media / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012501:Saphenous Vein / Q000166:cytology* / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
D003470:Culture Media; D015718:Fibrin Tissue Adhesive; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D003094:Collagen