title: A highly efficient protocol for micropropagation of Begonia tuberous.
abstract: A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 +/- 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.
authors.affiliation: Plant Molecular Biology and Plant Breeding Laboratory, Tay Nguyen Institute of Biology, Dalat, Lam Dong, Vietnam.
authors.forename: Tan Nhut
authors.initials: TN
authors.lastname: Duong
authors.forename: Thanh Hai
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Xuan Phan
authors.initials: XP
authors.lastname: Mai
mesh_terms: D000064:Acclimatization; D031112:Begoniaceae / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D049109:Cell Proliferation; D046508:Culture Techniques; D038081:Organogenesis; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D012038:Regeneration; D013997:Time Factors
chemical_list: D010937:Plant Growth Regulators