title: Molecular identification and full genome analysis of an echovirus 7 strain isolated from the environment in Greece.
abstract: Two enteroviruses from river water and four from sewage treatment plant were isolated in Larissa, Greece, that all shared the same sequence. A full genome analysis was conducted in an attempt to reveal the evolutionary pathways of one of the isolated strains (LR11F7). VP1 nucleotide and phylogenetic analysis revealed that the isolated strain had 78% homology with the echovirus 7 prototype strain Wallace. Full genome analysis revealed that LR11F7 P1 region is related to echoviruses 7 and that P2 and P3 regions are originating from contemporary enteroviruses isolated in South Asia. Two recombination events were shown to be involved into the evolutionary history of LR11F7, the one event concerning 3A, 3B, and 2C, and the other concerning 3D genomic region, both with new types of HEV-B. The contribution of recombination to enterovirus evolution is substantial, giving rise to new genetic lineages with unknown properties.
authors.affiliation: Department of Biochemistry & Biotechnology, University of Thessaly, 26 Ploutonos & Aeolou Str, Larissa 41221, Greece.
authors.forename: Zaharoula
authors.initials: Z
authors.lastname: Kyriakopoulou
authors.forename: Evaggelos
authors.initials: E
authors.lastname: Dedepsidis
authors.forename: Vaia
authors.initials: V
authors.lastname: Pliaka
authors.forename: Panayotis
authors.initials: P
authors.lastname: Mastorakos
authors.forename: Anastassia
authors.initials: A
authors.lastname: Stamati
authors.forename: Anastassia
authors.initials: A
authors.lastname: Pratti
authors.forename: Stamatina
authors.initials: S
authors.lastname: Levidiotou-Stefanou
authors.forename: Panayotis
authors.initials: P
authors.lastname: Markoulatos
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005618:Fresh Water / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006115:Greece; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D045483:Rivers; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral