Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration.
OBJECTIVE
To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation.


METHODS
We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed.


RESULTS
Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect.


CONCLUSIONS
Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.
Azar
Dimitri T
DT
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USA. dazar@uic.edu
Casanova
Fabio H
FH
Mimura
Tatsuya
T
Jain
Sandeep
S
Zhou
Zhongjun
Z
Han
Kyu Yeon
KY
Chang
Jin-Hong
JH
D020533:Angiogenesis Inhibitors / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002461:Cell Line, Transformed; D002465:Cell Movement; D049109:Cell Proliferation; D004730:Endothelium, Vascular / Q000166:cytology*; D019573:Epithelium, Corneal / Q000201:enzymology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D053511:Matrix Metalloproteinase 14 / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011651:Pulmonary Artery / Q000166:cytology; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D020533:Angiogenesis Inhibitors; C507096:Mmp14 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D053511:Matrix Metalloproteinase 14