title: Identification of rare and novel Cryptosporidium GP60 subtypes in human isolates from Jordan.
abstract: Little is known about the epidemiology of Cryptosporidium in Jordan and no genotyping studies have been conducted on Cryptosporidium isolates from humans or animals from Jordan. Genotyping of 44 Cryptosporidium isolates from Jordanian children at the 18S rRNA locus and a unique diagnostic locus identified four Cryptosporidium species; C. parvum (22), C. hominis (20), C. meleagridis (1) and C. canis (1). Sub-genotype analysis of 29 isolates at the 60-kDa glycoprotein (GP60) locus identified three C. parvum, two C. hominis subtype families and one C. meleagridis subtype. Several rare and novel subtypes were identified indicating unique endemicity and transmission of Cryptosporidium in Jordan.
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, The Hashemite University, P.O. Box 150459, Zarqa 13115, Jordan.
authors.forename: Nawal
authors.initials: N
authors.lastname: Hijjawi
authors.forename: Josephine
authors.initials: J
authors.lastname: Ng
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.forename: Manar F M
authors.initials: MF
authors.lastname: Atoum
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D015746:Abdominal Pain; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry; D003967:Diarrhea; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007223:Infant; D007597:Jordan / Q000453:epidemiology; D008969:Molecular Sequence Data; D015995:Prevalence; D014839:Vomiting
chemical_list: D016054:DNA, Protozoan