title: Evaluation of hepatitis B virus promoters for sustained transgene expression in mice by bioluminescence imaging.
abstract: To find new liver-specific expression cassettes for long-term expression of therapeutic genes in the context of pDNA, the function and specificity of hepatitis B virus (HBV)' two hepatic enhancers (EnI and EnII), combined with HBV core and X promoters in cultured cells were evaluated. By bioluminescence imaging and hydrodynamic gene transfer technology, the persistence of transgene expression containing these regulatory sequences in the liver of mice was assessed. Our data indicated that both HBV enhancers were able to stimulate HBV core and X promoter activity in cultured cells of hepatic origin. In vivo, HBV core promoter linked to EnI and EnII (EII-EI-Pc) and X promoter linked to EnI and EnII (EI-EII-Px) could direct a constant and high-level gene expression.
authors.affiliation: Beijing Institute of Transfusion Medicine, Tai ping Road 27#, Beijing 100850, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.forename: Sheng-qiang
authors.initials: SQ
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying-li
authors.initials: YL
authors.lastname: Wang
authors.forename: Hu
authors.initials: H
authors.lastname: Yan
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Hai-ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: Zhan
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D017930:Genes, Reporter; D006515:Hepatitis B virus / Q000235:genetics*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic; D019076:Transgenes; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D049410:Luciferases, Renilla