title: Amino acid substitution at the substrate-binding subsite alters the specificity of the Phanerochaete chrysosporium cellobiose dehydrogenase.
abstract: The active site of cellobiose dehydrogenase from Phanerochaete chrysosporium is composed of two subsites, a catalytic C subsite and a substrate-binding B subsite. Based on the crystal structure of the enzyme with a cellobiose analogue, residue Glu279 was selected for site-directed mutagenesis studies. Substitution of Glu279 to Ala, Asn, and Asp had no effect on the expression of the protein in Pichia pastoris but completely abolished its enzymatic activity. Substitution of Glu279 to Gln drastically altered the enzyme's substrate specificity. While the wild-type cellobiose dehydrogenase efficiently oxidizes cellobiose and lactose, the Glu279Gln mutant retained most of its activity with cellobiose but was completely inactive with lactose. We generated structural models of the active site interacting with cellobiose and lactose to provide an interpretation of these results.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. 50006831704@st.tuat.ac.jp
authors.lastname: Desriani
authors.forename: Stefano
authors.initials: S
authors.lastname: Ferri
authors.forename: Koji
authors.initials: K
authors.lastname: Sode
mesh_terms: D019943:Amino Acid Substitution; D001665:Binding Sites / Q000235:genetics; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005415:Flavins / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology*; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity / Q000235:genetics
chemical_list: D005415:Flavins; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D007785:Lactose