title: Bowman-Birk inhibitor affects pathways associated with energy metabolism in Drosophila melanogaster.
abstract: Bowman-Birk inhibitor (BBI) is toxic when fed to certain insects, including the fruit fly, Drosophila melanogaster. Dietary BBI has been demonstrated to slow growth and increase insect mortality by inhibiting the digestive enzymes trypsin and chymotrypsin, resulting in a reduced supply of amino acids. In mammals, BBI influences cellular energy metabolism. Therefore, we tested the hypothesis that dietary BBI affects energy-associated pathways in the D. melanogaster midgut. Through microarray and metabolomic analyses, we show that dietary BBI affects energy utilization pathways in the midgut cells of D. melanogaster. In addition, ultrastructure studies indicate that microvilli are significantly shortened in BBI-fed larvae. These data provide further insights into the complex cellular response of insects to dietary protease inhibitors.
authors.affiliation: Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: H-M
authors.initials: HM
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: O
authors.initials: O
authors.lastname: Mittapalli
authors.forename: W M
authors.initials: WM
authors.lastname: Muir
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: B J
authors.initials: BJ
authors.lastname: Schemerhorn
authors.forename: A
authors.initials: A
authors.lastname: Jannasch
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Adamec
authors.forename: L L
authors.initials: LL
authors.lastname: Murdock
authors.forename: B R
authors.initials: BR
authors.lastname: Pittendrigh
mesh_terms: D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004734:Energy Metabolism / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D020869:Gene Expression Profiling; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D055432:Metabolomics; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology*
chemical_list: D014157:Transcription Factors; D014358:Trypsin Inhibitor, Bowman-Birk Soybean