Development of an immunochromatographic strip for the detection of antibodies against foot-and-mouth disease virus serotype O.
An immunochromatographic strip was developed for the serological detection of type O foot-and-mouth disease (FMD) in swine. In the strip, the expressed protein of VP1, the main protective antigen of FMDV, labeled with colloidal gold was used as the detector, the staphylococcal protein A (SPA) and swine anti-foot-and-mouth disease virus (FMDV) antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. 296 swine serum samples were collected to evaluate the characteristics of the strip in comparison with existing commercial liquid-phage blocking ELISA (LPB ELISA) kit and peptide ELISA kit. The strip was shown to be of high specificity and sensitivity. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents nor equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.
Yang
Suzhen
S
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Yang
Jifei
J
Zhang
Gaiping
G
Wang
Xuannian
X
Qiao
Songlin
S
Zhao
Dong
D
Zhi
Yubao
Y
Li
Xuewu
X
Xing
Guangxu
G
Luo
Jun
J
Fan
Jianming
J
Bao
Dengke
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000276:immunology; D002845:Chromatography / Q000379:methods*; D003101:Collodion; D005536:Foot-and-Mouth Disease / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology / Q000302:isolation & purification*; D017979:Gold Colloid; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000821:virology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D017979:Gold Colloid; D011934:Reagent Strips; C442501:VP1 protein, Foot-and-mouth disease virus; D003101:Collodion