title: Expression of hepaCAM and its effect on proliferation of tumor cells in renal cell carcinoma.
abstract: OBJECTIVE
  To evaluate hepaCAM (hepatocyte cell adhesion molecule) gene expression in patients with renal cell carcinoma (RCC) and to explore its effect on proliferation of 786-0 cells. hepaCAM is a tumor suppressor gene, which has been identified as a member of immunoglobulin superfamily cell adhesion molecule.
  
  
  METHODS
  Two-step reverse transcription-polymerase chain reaction was used to determine hepaCAM expression in 30 paired (RCC and the adjacent non-RCC) renal specimens. Transfection studies were carried out by expressing green fluorescent protein and green fluorescent protein-fused hepaCAM in 786-0 cells.
  
  
  RESULTS
  Significant downregulation of hepaCAM was detected in 25 of 30 RCC patients tested. When transfected into 786-0 cells, the number of colony formation was reduced by 5-fold according to colony formation assay. MTT (3-diphenyltetrazolium bromide) showed the inhibition rates on the fourth, fifth, and sixth days of culturing were 26.5%, 38.1%, and 35.7%, respectively.
  
  
  CONCLUSIONS
  Our data show that hepaCAM is frequently downregulated in RCC, and that exogenous hepaCAM exhibits antiproliferative effect on 786-0 cells, suggesting that silencing of hepaCAM may be associated with carcinogenesis of RCC.
authors.affiliation: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Xun
authors.forename: Chunli
authors.initials: C
authors.lastname: Luo
authors.forename: Xiaohou
authors.initials: X
authors.lastname: Wu
authors.forename: Qiaolin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Yan
authors.forename: Shali
authors.initials: S
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology*; D018797:Cell Cycle Proteins; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; C487620:HEPACAM protein, human; D011506:Proteins