[Effects of recombinant Balillus Calmette-Guerin secreting IL-12 vaccination on development of T cell subsets in neonatal BALB/c mice].
OBJECTIVE
To explore the effects of recombinant Balillus Calmette-Guerin secreting IL-12 on the development of spleen T cell subsets of neonatal BALB/c mice.


METHODS
Twenty-four neonatal BALB/c mice were divided 3 groups: Control, BCG, r-BCG groups, which inoculated with BCG and r-BCG intraperiotoneally within 2-3 days after birth. Four weeks later, spleen cells of mice were isolated and the percentage of CD3(+) CD8(+) INF-gamma(+)/CD3(+) CD8(-) IFN-gamma(+)/CD3(+) CD8(+) IL-4(+)/CD3(+) CD8(-) IL-4(+)/ CD4(+) Foxp3(+) T cells, which represent Tc1/Th1/Tc2/Th2/Treg cells respectively were detected by flow cytometry at single cells level, and Th1/Th2, Tc1/Tc2 ratio were calculated.


RESULTS
The percentages of Th1, Tc1 cell of BCG-vaccinated mice, r-BCG -vaccinated mice were significant higher than control mice (P < 0.01), and there was no difference between the two vaccinated groups. The ratio of Th1/Th2, Tc1/Tc2 and total IFN-gamma/IL-4 of two vaccinated groups were higher than control group. The percentages of Treg cells were no difference among the three groups (P > 0.05).


CONCLUSIONS
Neonatal BCG and r-BCG vaccination can significantly increase Tc1 and Th1 cells and Th1/Th2, Tc1/Tc2 ratio in spleen cells. Neonatal BCG and r-BCG vaccination may have an same effects on development of T cell subsets in neonatal BALB/c mice. The percentage of CD4(+) Foxp3(+) T cells among three groups were no significant difference.
He
Yun
Y
Department of Clinical Immunology Children's Hospital, Chongqing Medical University, China. ncheyun@163.com
Liu
En-mei
EM
Yang
Xi-qiang
XQ
Li
Xin
X
Liu
Wei
W
D000818:Animals; D000831:Animals, Newborn; D001500:BCG Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D018664:Interleukin-12 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D011897:Random Allocation; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D001500:BCG Vaccine; D014614:Vaccines, Synthetic; D018664:Interleukin-12