Quality assessment of a placental perfusion protocol.
Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3 ml h(-1) from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100 ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.
Mathiesen
Line
L
Institute of Public Health, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
Mose
Tina
T
Mørck
Thit Juul
TJ
Nielsen
Jeanette Kolstrup Søgaard
JK
Nielsen
Leif Kofoed
LK
Maroun
Lisa Leth
LL
Dziegiel
Morten Hanefeld
MH
Larsen
Lise Grupe
LG
Knudsen
Lisbeth Ehlert
LE
D000826:Animal Testing Alternatives; D000983:Antipyrine / Q000493:pharmacokinetics; D004867:Equipment Design; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008431:Maternal-Fetal Exchange; D010477:Perfusion / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011786:Quality Control; D012098:Reproduction / Q000187:drug effects*; D018675:Toxicity Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards*
D000983:Antipyrine