Medium optimization for phenazine-1-carboxylic acid production by a gacA qscR double mutant of Pseudomonas sp. M18 using response surface methodology.
Statistics based experimental designs were applied to optimize the culture medium components for enhancing phenazine-1-carboxylic acid (PCA) production by Pseudomonas sp. strain M18GQ, a gacA qscR double mutant of Pseudomonas sp. strain M18. The medium components, including soybean meal, glucose and corn steep liquor, had significant effects on PCA production based on a 2(5-1) fractional factorial design. The concentrations of these three significant factors were subsequently optimized using a central composite design. An optimum concentration of soybean meal 73.3g/L, corn steep liquor 18.1g/L, glucose 17.9g/L, and ethanol 18.0ml/L was obtained by response surface analysis. The predicted maximum PCA yield was as high as 4011.5mg/L, and was confirmed by validation experiments. The double mutant M18GQ produced 4032.2mg/L PCA, which was almost 2 to 3.3-fold that produced by either single mutant M18G and M18Q. The achieved production level is economically useful for industrial application.
Su
Jin-Jiang
JJ
Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Zhou
Quan
Q
Zhang
Hong-Yan
HY
Li
Ya-Qian
YQ
Huang
Xian-Qing
XQ
Xu
Yu-Quan
YQ
D009154:Mutation; D010619:Phenazines / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max
D010619:Phenazines; C037165:1-phenazinecarboxylic acid