Effects of nonionic surfactant Triton X-100 on the laccase-catalyzed conversion of bisphenol A.
The laccase-catalyzed conversion of bisphenol A (BPA) in aqueous solutions was studied in the absence and presence of nonionic surfactant Triton X-100. It was found that the addition of Triton X-100 into the reaction system increased the conversion of BPA, especially near the critical micelle concentration of Triton X-100. Also it was found that the stability of laccase was greatly improved in the presence of TritonX-100. Studies on the endogenous fluorescence emission of laccase indicated that there existed an interaction between Triton X-100 and laccase, which was beneficial to folding and stabilizating of laccase. The binding of Triton X-100 to the laccase surface also mitigated the inactivation effect caused by the free radicals and polymerization products. Under otherwise identical conditions, a lower dosage of laccase was needed for the higher conversion of BPA in the presence of Triton X-100.
Ji
Guanglei
G
School of Environmental Science and Engineering, Shandong University, Jinan 250100, China. chm_jigl@ujn.edu.cn
Zhang
Haibo
H
Huang
Feng
F
Huang
Xirong
X
D001559:Benzhydryl Compounds; D042845:Laccase / Q000378:metabolism*; D017830:Octoxynol / Q000737:chemistry*; D010636:Phenols / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D042845:Laccase; D017830:Octoxynol; D010636:Phenols; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds