[Role of cholera toxin-induced apoptosis in alteration of macrophages in mice].
OBJECTIVE
To study the role of active programmed cell death induced by Vibrio cholerae antigens in alteration of peritoneal macrophages of experimental animals.


METHODS
Apoptosis was assessed by cytofluorometric analysis with propidium iodide using cytofluorometer "Coulter" as well as on characteristic morphological changes of cells in stained histological preparations.


RESULTS
Performed experiments carried out by both methods provide evidence that V. cholerae and its antigens (cholera toxin, neuraminidase, chitinase, and lypopolysaccharide) cause apoptosis of mice peritoneal macrophages, which leads to their alteration.


CONCLUSIONS
Our results demonstrate that programmed cell death of phagocytes is one of the causes of cytotoxic effect of V.cholerae and its antigens. Performed experiments show the role of apoptosis of macrophages in formation of postimmunization immunosuppression after vaccination against cholera.
Ivanova
I A
IA
Vasil'eva
G I
GI
Mishan'kin
B N
BN
Kiseleva
A K
AK
Doroshenko
E P
EP
Bespalova
I A
IA
Shimaniuk
N Ia
NIa
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D017209:Apoptosis / Q000276:immunology*; D002771:Cholera / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D014734:Vibrio cholerae / Q000276:immunology*
D000942:Antigens, Bacterial; D002772:Cholera Toxin