title: Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes.
abstract: The minor pilin FctB is an integral part of the pilus assembly expressed by Streptococcus pyogenes. Since it is located at the cell wall, it can be hypothesized that it functions as a cell-wall anchor for the streptococcal pilus. In order to elucidate its structure, the genes for FctB from the S. pyogenes strains 90/306S and SF370 were cloned for overexpression in Escherichia coli. FctB from strain 90/306S was crystallized by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. The hexagonal FctB crystals belonged to space group P6(1) or P6(5), with unit-cell parameters a = b = 95.15, c = 100.25 A, and diffracted to 2.9 A resolution.
authors.affiliation: School of Biological Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: Christian
authors.initials: C
authors.lastname: Linke
authors.forename: Paul G
authors.initials: PG
authors.lastname: Young
authors.forename: Hae Joo
authors.initials: HJ
authors.lastname: Kang
authors.forename: Thomas
authors.initials: T
authors.lastname: Proft
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
mesh_terms: D003001:Cloning, Molecular; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013297:Streptococcus pyogenes / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D036781:Fimbriae Proteins