title: Live birth after ovarian tissue autograft in a patient with sickle cell disease treated by allogeneic bone marrow transplantation.
abstract: OBJECTIVE
  To report the first case of restoration of ovarian activity and live birth after cryopreserved ovarian tissue autograft in a patient without cancer treated by allogeneic bone marrow transplantation.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  One woman with homozygous sickle cell anemia.
  
  
  METHODS
  An orthotopic autotransplantation of ovarian cortical strips was performed after freeze-thawing.
  
  
  METHODS
  Cryopreservation of ovarian tissue, bone marrow transplantation, ovarian autograft, and restoration of ovarian function.
  
  
  RESULTS
  In autumn 2005, biopsy samples of ovarian tissue were cryopreserved before chemotherapy followed by bone marrow transplantation. In spring 2008, because the patient had been menopausal for 2.5 years as a result of the conditioning therapy, an orthotopic autotransplantation of thawed ovarian cortex was performed. The patient conceived spontaneously in a natural cycle in autumn 2008, and delivered a healthy female child in June 2009.
  
  
  CONCLUSIONS
  Cryopreservation of ovarian tissue with subsequent autotransplantation is an emerging procedure for preserving the fertility of young patients with a high risk of premature ovarian failure (POF) resulting from gonadotoxic treatment. This case opens up new perspectives in cases of nonmalignant diseases.
authors.affiliation: Service de Génétique Histologie Biologie du Développement et de la Reproduction (CECOS Franche-Comté Bourgogne), Centre Hospitalier Universitaire, Université de Franche-Comté, Besançon, France. christophe.roux@univ-fcomte.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Roux
authors.forename: Clotilde
authors.initials: C
authors.lastname: Amiot
authors.forename: Germain
authors.initials: G
authors.lastname: Agnani
authors.forename: Yves
authors.initials: Y
authors.lastname: Aubard
authors.forename: Pierre-Simon
authors.initials: PS
authors.lastname: Rohrlich
authors.forename: Pascal
authors.initials: P
authors.lastname: Piver
mesh_terms: D000328:Adult; D000755:Anemia, Sickle Cell / Q000534:rehabilitation* / Q000628:therapy*; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000379:methods / Q000534:rehabilitation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D050498:Live Birth; D010053:Ovary / Q000601:surgery / Q000637:transplantation*; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000534:rehabilitation / Q000628:therapy; D016649:Primary Ovarian Insufficiency / Q000209:etiology / Q000534:rehabilitation / Q000628:therapy; D019172:Transplantation Conditioning / Q000009:adverse effects; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous / Q000009:adverse effects