title: Mossy fiber sprouting, hippocampal damage and spontaneous recurrent seizures in pentylenetetrazole kindling rat model.
abstract: OBJECTIVE
  The aim of this study was to determine the correlations among hippocampal damage, spontaneous recurrent seizures (SRS), and mossy fiber sprouting (MFS) using pentylenetetrazole (PTZ) kindling model.
  
  
  METHODS
  Chronic epileptic model was established by administration of PTZ. Behaviour and EEG seizure activity were recorded. Rats' hippocampus were analyzed with haematoxylin and eosin (H&E) stain for histological lesions and evaluated for MFS with Timm stain.
  
  
  RESULTS
  Prominent MFS was observed in area CA3 rather than the inner molecular layer in PTZ treated rats and the degree of MFS progressed with the development of behavioral kindled seizures. MFS preceded the occurrence of spontaneous seizures. No obvious neuronal necrosis and loss were observed in different regions of the hippocampus during kindling progression.
  
  
  CONCLUSIONS
  MFS is not the outcome of SRS. Severe hippocampal damage is not required in the development of MFS and SRS.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, China. tianfafa@gmail.com
authors.forename: Fa-Fa
authors.initials: FF
authors.lastname: Tian
authors.forename: Chang
authors.initials: C
authors.lastname: Zeng
authors.forename: Ting-Hui
authors.initials: TH
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Chen
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Ma
authors.forename: Jia
authors.initials: J
authors.lastname: Fang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Cai
authors.forename: Feng-Rong
authors.initials: FR
authors.lastname: Li
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Huang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Fu
authors.forename: Jing
authors.initials: J
authors.lastname: Dang
mesh_terms: D000818:Animals; D004569:Electroencephalography; D006624:Hippocampus / Q000473:pathology* / Q000503:physiopathology; D007696:Kindling, Neurologic / Q000473:pathology*; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000473:pathology* / Q000503:physiopathology; D009474:Neurons / Q000473:pathology; D010433:Pentylenetetrazole / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000473:pathology* / Q000503:physiopathology; D013194:Staining and Labeling; D018709:Statistics, Nonparametric
chemical_list: D010433:Pentylenetetrazole