Pharmacodynamic interaction of garlic with hydrochlorothiazide in rats.
The purpose of current study was to evaluate the influence of hydrochlorothiazide (HCTZ) on protective effects of garlic homogenate (GH) in rats subjected to myocardial damage induced by ischemia-reperfusion (IRI) and isoproterenol (ISO). Female Wistar albino rats were treated with GH at three different doses of 125; 250 and 500 mg/kg orally for 30 days and HCTZ (10 mg/kg, p.o.) was incorporated in the interactive groups during the last seven days of GH treatment. At the end of treatment, animals were subjected to IRI or ISO induced myocardial damage in their respective models. The GH 250 mg/kg was found to dislodge the effect of ISO and IRI on superoxide dismutase & catalase and retained the activities of LDH and CK-MB. Adding HCTZ improved biochemical, antioxidant and histological status of myocardium during myocardial damage. Since no toxic effects were observed, HCTZ may safely be used to improve GH therapy.
Asdaq
Syed Mohammed Basheeruddin
SM
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore - 560 035. basheer_1@rediffmail.com
Inamdar
Mohammed Naseeruddin
MN
D000284:Administration, Oral; D000818:Animals; D002317:Cardiovascular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005737:Garlic / Q000737:chemistry; D006331:Heart Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D041743:Herb-Drug Interactions; D006852:Hydrochlorothiazide / Q000008:administration & dosage / Q000494:pharmacology*; D007545:Isoproterenol; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D002317:Cardiovascular Agents; D010936:Plant Extracts; D006852:Hydrochlorothiazide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D052279:Creatine Kinase, MB Form; D007545:Isoproterenol