[Identify the original plant of Forsythia suspensa by nucleotide sequence].
OBJECTIVE
To investigate the means of distinguishing the original plant of Forsythia suspensa from confusion.


METHODS
To amplify the chloroplast matK gene by PCR using a consensus primer set and determine their nucleotide sequence by PCR direct sequencing. The ITS sequences were gained from NCBI. The characteristic analysis of matK and ITS sequences were generated using Clustal aligned.


RESULTS
There were 30 bp and 8 bp unique nucleotide in ITS and matK sequence in Forsythia suspensa. The matK gene and ITS sequences might be good molecular marker to be used to identify the original plant of Forsythia suspensa.


CONCLUSIONS
The sequence analysis of matK gene ITS sequences might become the mean to identify the original plant of Forsythia suspensa.
Yuan
Wang-Jun
WJ
Institute of Natural Products, Henan University, Kaifeng 475004, China. yuanwangjun@henu.edu.cn
Xu
Shou-Ming
SM
Zhang
Wei-Rui
WR
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018742:DNA, Chloroplast / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics*; D031657:Forsythia / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D018742:DNA, Chloroplast; D018744:DNA, Plant; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases