[Systemic amyloidosis: practical diagnosis].
Amyloid deposits have particular ultrastructural appearance with 7 to 10 nm-diameter fibrils. Amyloid is defined by its tinctorial affinity, which includes Congo red positivity, which must polarize and produce apple-green birefringence. Immunohistochemical characterization allows to know the origin of amyloidosis (characterization, which must be performed on frozen tissue). This restatement is proposed to renal pathologists in indicating the traps of studying amyloidosis, the tissues performing for the diagnosis, the specific staining and the immunohistochemical characterization.
Noël
Laure-Hélène
LH
Inserm U 845, hôpital Necker, 161, rue de Sèvres, 75015 Paris, France. laure-helene.noel@inserm.fr
D000686:Amyloidosis / Q000175:diagnosis*; D001718:Birefringence; D004396:Coloring Agents; D003224:Congo Red; D003937:Diagnosis, Differential; D006651:Histocytochemistry; D006801:Humans; D008859:Microscopy, Polarization; D011379:Prognosis; D013194:Staining and Labeling
D004396:Coloring Agents; D003224:Congo Red