title: Toxicity comparison of chlorinated and brominated dibenzo-p-dioxins and dibenzofurans in industrial source samples by HRGC/HRMS and enzyme immunoassay.
abstract: Limited information is available on the applicability of polychlorinated dibenzo-p-dioxin/furan (PCDD/F) toxicity assays to their brominated counterparts: polybrominated dibenzo-p-dioxins/furans (PBDDs/Fs). We estimated the toxicity of mixtures of chlorinated, brominated, and mixed bromochloro-dioxins and -furan (PBCDDs/Fs) laboratory standards using a chemically-activated luciferase gene expression cell bioassay (CALUX). The relative effects potency (REP) values obtained were comparable to the World Health Organization (WHO) toxic equivalency factors (TEFs) and in agreement with the concept of additive congener toxicity of mixtures of dioxins and furans. Enzyme immunoassay (EIA)-based toxic equivalents (TEQs), however, showed overestimation for PCDDs/Fs (0-4 orders of magnitudes higher) and underestimation for PBDDs/Fs (0-1 orders of magnitude lower) when compared to high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS)-based TEQ calculation (using WHO TEFs) in samples from an industrial source line. No correlation was found between the EIA and the HRGC/HRMS data, which could be attributed to differences in homologue-specific cross-reactivity responses, sample matrix type, and presence of other compounds competing for antibody binding in the immunoassay.
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 109 T.W. Alexander Drive, Research Triangle Park, NC 27711, USA.
authors.forename: Fatin
authors.initials: F
authors.lastname: Samara
authors.forename: Barbara
authors.initials: B
authors.lastname: Wyrzykowska
authors.forename: Dennis
authors.initials: D
authors.lastname: Tabor
authors.forename: Dahman
authors.initials: D
authors.lastname: Touati
authors.forename: Brian K
authors.initials: BK
authors.lastname: Gullett
mesh_terms: D001572:Benzofurans / Q000032:analysis*; D001681:Biological Assay / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D004147:Dioxins / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006842:Hydrocarbons, Brominated / Q000032:analysis*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D007220:Industrial Waste; D013058:Mass Spectrometry / Q000379:methods; D013223:Statistics as Topic
chemical_list: D001572:Benzofurans; D004147:Dioxins; D004785:Environmental Pollutants; D006842:Hydrocarbons, Brominated; D006843:Hydrocarbons, Chlorinated; D007220:Industrial Waste; C023614:dibenzofuran; C020887:dibenzo(1,4)dioxin