title: [Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008].
abstract: OBJECTIVE
  To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene.
  
  
  METHODS
  Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4.
  
  
  RESULTS
  The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain.
  
  
  CONCLUSIONS
  Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Gao
authors.forename: You-Gang
authors.initials: YG
authors.lastname: Zhai
authors.forename: De-Shan
authors.initials: DS
authors.lastname: Yu
authors.forename: Guo-Tai
authors.initials: GT
authors.lastname: Li
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Jia
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002681:China; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins