[Study on extraction and purification process of total ginsenosides from Radix Ginseng].
OBJECTIVE
To optimize the technological parameters of the extraction and purification process of total ginsenosides from Radix Ginseng.


METHODS
With the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1, the orthogonal design was adopted to optimize the extraction process. The purification process was studied by optimizing the elutive ratio of total ginsenosides as the marker. HPLC and spectrophotometer were employed for the study.


RESULTS
The optimum conditions were as follows:Using 8 times volume of 75% ethanol extracting for 120 minutes and 2 times, the extraction temperature was 85 degrees C. AB-8 macroporous resin was selected, and the eluant was 4 BV 70% ethanol.


CONCLUSIONS
The optimal conditions of extracting and purifying the total ginsenosides from Radix Ginseng is feasible.
Xie
Li-Ling
LL
Department of Biology, Shantou University, Shantou 515063, China. llxie@stu.edu.cn
Ren
Li
L
Lai
Xian-Sheng
XS
Cao
Jun-Hui
JH
Mo
Quan-Yi
QY
Chen
Wei-Wen
WW
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D036145:Ginsenosides / Q000032:analysis / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012117:Resins, Synthetic; D012997:Solvents; D000431:Ethanol; C049864:ginsenoside Re; C035054:ginsenoside Rg1