The submergence tolerance regulator Sub1A mediates stress-responsive expression of AP2/ERF transcription factors.
We previously characterized the rice (Oryza sativa) Submergence1 (Sub1) locus encoding three ethylene-responsive factor (ERF) transcriptional regulators. Genotypes carrying the Sub1A-1 allele are tolerant of prolonged submergence. To elucidate the mechanism of Sub1A-1-mediated tolerance, we performed transcriptome analyses comparing the temporal submergence response of Sub1A-1-containing tolerant M202(Sub1) with the intolerant isoline M202 lacking this gene. We identified 898 genes displaying Sub1A-1-dependent regulation. Integration of the expression data with publicly available metabolic pathway data identified submergence tolerance-associated pathways governing anaerobic respiration, hormone responses, and antioxidant systems. Of particular interest were a set of APETALA2 (AP2)/ERF family transcriptional regulators that are associated with the Sub1A-1-mediated response upon submergence. Visualization of expression patterns of the AP2/ERF superfamily members in a phylogenetic context resolved 12 submergence-regulated AP2/ERFs into three putative functional groups: (1) anaerobic respiration and cytokinin-mediated delay in senescence via ethylene accumulation during submergence (three ERFs); (2) negative regulation of ethylene-dependent gene expression (five ERFs); and (3) negative regulation of gibberellin-mediated shoot elongation (four ERFs). These results confirm that the presence of Sub1A-1 impacts multiple pathways of response to submergence.
Jung
Ki-Hong
KH
Department of Plant Pathology, University of California, Davis, California 95616, USA.
Seo
Young-Su
YS
Walia
Harkamal
H
Cao
Peijian
P
Fukao
Takeshi
T
Canlas
Patrick E
PE
Amonpant
Fawn
F
Bailey-Serres
Julia
J
Ronald
Pamela C
PC
D000064:Acclimatization; D016000:Cluster Analysis; D055868:Floods; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000032:analysis; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014867:Water
D010940:Plant Proteins; D014157:Transcription Factors; D014867:Water; D006861:Hydrogen Peroxide