title: Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols.
abstract: Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
authors.affiliation: Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Gil Ah
authors.initials: GA
authors.lastname: Kim
authors.affiliation: Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Hwa Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Jee Woong
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: Gene
authors.initials: G
authors.lastname: Lee
authors.affiliation: School of Veterinary Medicine, Kangwon National University, Chunchon, South Korea.
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.affiliation: Research Institute for Agriculture and Life Science, Seoul National University, Seoul, South Korea. Electronic address: limjm@snu.ac.kr.
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005615:Freezing / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D006080:Ovarian Follicle / Q000473:pathology / Q000648:ultrastructure*; D010053:Ovary; D015044:Zona Pellucida / Q000473:pathology / Q000648:ultrastructure