Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier.
An aerobic, Gram-negative, yellow-pigmented bacterial strain, designated 0533(T), was isolated from frozen soil from the China No. 1 glacier. Phylogenetic analysis of the 16S rRNA gene sequence demonstrated that strain 0533(T) was a member of the genus Flavobacterium and exhibited 97.1-98.7 % 16S rRNA sequence similarity with its nearest phylogenetic neighbours. Strain 0533(T) exhibited phenotypic and chemotaxonomic characteristics common to the genus Flavobacterium: menaquinone-6 (MK-6) was the predominant quinone and iso-C(15 : 0), C(17 : 1)ω6c, anteiso-C(15 : 0), iso-C(15 : 0) 3-OH, C(15 : 1)ω6c, iso-C(16 : 0) 3-OH, summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), iso-C(15 : 1) G and iso-C(17 : 0) 3-OH were the major fatty acids (>5 %). The DNA G+C content was 32.5 mol%. On the basis of phenotypic and genotypic data, a novel species, Flavobacterium sinopsychrotolerans sp. nov., is proposed. The type strain is 0533(T) (=CGMCC 1.8704(T) =JCM 16398(T)).
Xu
Mingshuang
M
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Xin
Yuhua
Y
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Tian
Jiesheng
J
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Dong
Kun
K
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Yu
Yong
Y
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Zhang
Jianli
J
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Liu
Hongcan
H
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhou
Yuguang
Y
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2