title: Crypt abscess-associated microbiota in inflammatory bowel disease and acute self-limited colitis.
abstract: OBJECTIVE
  To evaluate whether crypt abscesses from inflammatory bowel disease (IBD) patients contain bacteria and to establish their nature.
  
  
  METHODS
  We studied 17 ulcerative colitis patients, 11 Crohn's disease patients, 7 patients with acute self-limited colitis (ASLC) and normal colonic biopsies from 5 subjects who underwent colonoscopy for colon cancer screening. A fluorescent in situ hybridization technique was applied to colonic biopsies to assess the microbiota composition of the crypts and crypt abscesses.
  
  
  RESULTS
  Crypts colonized by bacteria were observed in 42.9% and 3.6% of ASLC and IBD patients, respectively (P = 0.019). Crypt abscesses colonized by bacteria were observed in 28.6% and 0.0% of ASLC and IBD patients, respectively (P = 0.035).
  
  
  CONCLUSIONS
  These results do not support the hypothesis that crypt abscesses in IBD are the result of localized dysbiosis arising from persistence of living bacteria colonizing the crypts.
authors.affiliation: Gastroenterology and Nutrition Department, Saint Antoine Hospital, Pierre et Marie Curie-Paris6 University, AP-HP, 184 rue du Faubourg Saint-Antoine, 75571, Paris, France. sokol_harry@yahoo.fr
authors.forename: Harry
authors.initials: H
authors.lastname: Sokol
authors.forename: Nadia
authors.initials: N
authors.lastname: Vasquez
authors.forename: Nadia
authors.initials: N
authors.lastname: Hoyeau-Idrissi
authors.forename: Philippe
authors.initials: P
authors.lastname: Seksik
authors.forename: Laurent
authors.initials: L
authors.lastname: Beaugerie
authors.forename: Anne
authors.initials: A
authors.lastname: Lavergne-Slove
authors.forename: Philippe
authors.initials: P
authors.lastname: Pochart
authors.forename: Philippe
authors.initials: P
authors.lastname: Marteau
mesh_terms: D000038:Abscess / Q000382:microbiology* / Q000473:pathology; D000328:Adult; D003092:Colitis / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000382:microbiology / Q000473:pathology; D003113:Colonoscopy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015212:Inflammatory Bowel Diseases / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged