Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores.
We present a novel method for integrating two single-molecule measurement modalities, namely, total internal reflection microscopy and electrical detection of biomolecules using nanopores. Demonstrated here is the electrical measurement of nanopore based biosensing performed simultaneously and in-sync with optical detection of analytes. This method makes it possible, for the first time, to visualize DNA and DNA-protein complexes translocating through a nanopore with high temporal resolution (1000 frames/s) and good signal to background. This paper describes a detailed experimental design of custom optics and data acquisition hardware to achieve simultaneous high resolution electrical and optical measurements on labeled biomolecules as they traverse through a approximately 4 nm synthetic pore. In conclusion, we discuss new directions and measurements, which this technique opens up.
Soni
Gautam V
GV
Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Singer
Alon
A
Yu
Zhiliang
Z
Sun
Yingjie
Y
McNally
Ben
B
Meller
Amit
A
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D055615:Electrical Equipment and Supplies; D004867:Equipment Design; D008853:Microscopy / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D055096:Optical Devices; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
D011506:Proteins; D004247:DNA