Effect of dietary Polyphenon E and EGCG on lung tumorigenesis in A/J Mice.
OBJECTIVE
To compare the chemopreventive efficacy of Polyphenon E (Poly E), (-)-epigallocatechin-3-gallate (EGCG) and Polyphenon E without EGCG (Poly E-EGCG) on the development of benzo(a)pyrene (B(a)P)-induced lung tumors in A/J mice.


METHODS
Female A/J mice were given a single intraperitoneal injection of B(a)P (100 mg/kg body weight). One week after B(a)P injection, animals received AIN-76A purified powder diet containing 0.975% (wt/wt) EGCG, 0.525% (wt/wt) Poly E-EGCG or 1.5% (wt/wt) Poly E for 24 weeks or control diet with no additives.


RESULTS
Poly E treatment significantly decreased tumor multiplicity by 52% and tumor load by 64%, while EGCG and Poly E-EGCG did not significantly inhibit lung tumor multiplicity. EGCG was more stable in a complex mixture (Poly E) than as a pure compound.


CONCLUSIONS
EGCG was ineffective when administered by diet likely due to its instability. Thus, EGCG's efficacy on mice lung tumorigenesis requires the presence of other tea catechins.
Zhang
Qi
Q
Department of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis, 660 South Euclid Avenue, Campus Box 8109, St Louis, Missouri 63110, USA.
Fu
Huijing
H
Pan
Jing
J
He
Jun
J
Ryota
Seto
S
Hara
Yukihiko
Y
Wang
Yian
Y
Lubet
Ronald A
RA
You
Ming
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D018890:Chemoprevention; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008175:Lung Neoplasms / Q000178:diet therapy / Q000473:pathology / Q000517:prevention & control*; D051379:Mice
D016588:Anticarcinogenic Agents; D002392:Catechin; C045651:epigallocatechin gallate; C472086:polyphenon E