Microarray-based transcriptional profiling of renieramycin M and jorunnamycin C, isolated from Thai marine organisms.
Renieramycin M and jorunnamycin C, two isoquinolinequinone compounds differing only at the C-22 ester side chain, were evaluated for their cytotoxic effects on human colon (HCT116) and breast (MDA-MB-435) cancer cell lines. These two compounds displayed potent cancer cell growth inhibition, their IC(50) values reaching nanomolar order. To examine their effects on transcription, we carried out oligonucleotide microarray analysis with focus on the similarities and differences between the two compounds in terms of transcriptional profiles. We found that the down-regulation of PTPRK (protein tyrosine phosphatase receptor type K) can be considered as a biomarker responsive to the cytotoxic effects of this class of antitumor marine natural products.
Charupant
Kornvika
K
Department of Pharmacognocy and Pharmaceutical Botany, Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
Suwanborirux
Khanit
K
Daikuhara
Naomi
N
Yokoya
Masashi
M
Ushijima-Sugano
Rie
R
Kawai
Takatoshi
T
Owa
Takashi
T
Saito
Naoki
N
D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D004354:Drug Screening Assays, Antitumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000302:isolation & purification* / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D011809:Quinones / Q000302:isolation & purification* / Q000494:pharmacology; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000378:metabolism; D044005:Tetrahydroisoquinolines / Q000302:isolation & purification* / Q000494:pharmacology; D013785:Thailand; D014158:Transcription, Genetic
D000970:Antineoplastic Agents; D007546:Isoquinolines; D011809:Quinones; D044005:Tetrahydroisoquinolines; C550260:jorunnamycin C; C482478:renieramycin M; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2