title: [Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene].
abstract: OBJECTIVE
  To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope.
  
  
  METHODS
  An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT.
  
  
  RESULTS
  HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay.
  
  
  CONCLUSIONS
  A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.
authors.affiliation: Shenzhen Third People's Hospital, Shenzhen 518020, China.
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Wei-Long
authors.initials: WL
authors.lastname: Liu
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Yang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Xiu-Yun
authors.initials: XY
authors.lastname: Zhu
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zhang
authors.forename: Bo-Ping
authors.initials: BP
authors.lastname: Zhou
authors.forename: Lonnie
authors.initials: L
authors.lastname: Lybarger
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D018984:Epitopes, T-Lymphocyte / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006183:H-2 Antigens / Q000235:genetics* / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D064532:Histocompatibility Antigen H-2D; D006801:Humans; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018984:Epitopes, T-Lymphocyte; D006183:H-2 Antigens; D006514:Hepatitis B Surface Antigens; D064532:Histocompatibility Antigen H-2D; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins