title: Polycomb complexes act redundantly to repress genomic repeats and genes.
abstract: Polycomb complexes establish chromatin modifications for maintaining gene repression and are essential for embryonic development in mice. Here we use pluripotent embryonic stem (ES) cells to demonstrate an unexpected redundancy between Polycomb-repressive complex 1 (PRC1) and PRC2 during the formation of differentiated cells. ES cells lacking the function of either PRC1 or PRC2 can differentiate into cells of the three germ layers, whereas simultaneous loss of PRC1 and PRC2 abrogates differentiation. On the molecular level, the differentiation defect is caused by the derepression of a set of genes that is redundantly repressed by PRC1 and PRC2 in ES cells. Furthermore, we find that genomic repeats are Polycomb targets and show that, in the absence of Polycomb complexes, endogenous murine leukemia virus elements can mobilize. This indicates a contribution of the Polycomb group system to the defense against parasitic DNA, and a potential role of genomic repeats in Polycomb-mediated gene regulation.
authors.affiliation: Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Leeb
authors.forename: Diego
authors.initials: D
authors.lastname: Pasini
authors.forename: Maria
authors.initials: M
authors.lastname: Novatchkova
authors.forename: Markus
authors.initials: M
authors.lastname: Jaritz
authors.forename: Kristian
authors.initials: K
authors.lastname: Helin
authors.forename: Anton
authors.initials: A
authors.lastname: Wutz
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing; D051379:Mice; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000378:metabolism; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D020079:Terminal Repeat Sequences
chemical_list: D046912:Multiprotein Complexes; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins