[Genetic analysis of a strain of adenovirus Ad7d2 isolated from an infant died of pneumonia].
OBJECTIVE
To analyze the genetic characteristics of a strain of adenovirus, Ad7d2, isolated from an infant died of severe pneumonia.


METHODS
Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia. The viral DNA was amplified by PCR and the products were sequenced.


RESULTS
One strain of virus was isolated and was named as BJ060316-1. Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2, which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993. Blast with deduced amino acid sequence showed that BJ060316-1 lost glutamine at site 253, and at the site 495 arginine replaced serine. For fiber gene, BJ060316-1 showed 99.7% homology with AB243118 Ad7 isolated in Japan in 2005 for 975 bp fragment.


CONCLUSIONS
Adenovirus Ad7d2 strain BJ060316-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.
Tang
Liu-ying
LY
Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China. tangly812@hotmail.com
Liu
Xiu-yun
XY
Xu
Wen-bo
WB
D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000821:virology*; D017809:Fatal Outcome; D006801:Humans; D007223:Infant; D008297:Male; D011014:Pneumonia / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins