Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols.
Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
Kim
Gil Ah
GA
Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Kim
Hwa Young
HY
Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Kim
Jee Woong
JW
Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Lee
Gene
G
Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea.
Lee
Eunsong
E
School of Veterinary Medicine, Kangwon National University, Chunchon, South Korea.
Lim
Jeong Mook
JM
Research Institute for Agriculture and Life Science, Seoul National University, Seoul, South Korea. Electronic address: limjm@snu.ac.kr.
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005615:Freezing / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D006080:Ovarian Follicle / Q000473:pathology / Q000648:ultrastructure*; D010053:Ovary; D015044:Zona Pellucida / Q000473:pathology / Q000648:ultrastructure