Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide).
We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. With this method, the conformation of long genomic DNA can be readily and reversibly switched between a very compact condensate and an unfolded macromolecule.
Chen
Ning
N
Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Zinchenko
Anatoly A
AA
Kidoaki
Satoru
S
Murata
Shizuaki
S
Yoshikawa
Kenichi
K
D000180:Acrylic Resins / Q000494:pharmacology*; D017122:Bacteriophage T4; D004247:DNA / Q000737:chemistry*; D016678:Genome / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D012996:Solutions; D013696:Temperature
D000180:Acrylic Resins; D012996:Solutions; C052970:poly-N-isopropylacrylamide; D004247:DNA