title: The first low microM SecA inhibitors.
abstract: SecA ATPase is a critical member of the Sec family, which is important in the translocation of membrane and secreted polypeptides/proteins in bacteria. Small molecule inhibitors can be very useful research tools as well as leads for future antimicrobial agent development. Based on previous virtual screening work, we optimized the structures of two hit compounds and obtained SecA ATPase inhibitors with IC(50) in the single digit micromolar range. These represent the first low micromolar synthetic inhibitors of bacterial SecA and will be very useful for mechanistic studies.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA 30302-4098, USA.
authors.forename: Weixuan
authors.initials: W
authors.lastname: Chen
authors.forename: Ying-ju
authors.initials: YJ
authors.lastname: Huang
authors.forename: Sushma Reddy
authors.initials: SR
authors.lastname: Gundala
authors.forename: Hsiuchin
authors.initials: H
authors.lastname: Yang
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
authors.forename: Phang C
authors.initials: PC
authors.lastname: Tai
authors.forename: Binghe
authors.initials: B
authors.lastname: Wang
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008962:Models, Theoretical; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins