title: In vitro screening of essential oil from young and mature leaves of Artemisia scoparia compared to its major constituents for free radical scavenging activity.
abstract: The present study investigated the chemical characterization, and antioxidant activity of essential oil hydrodistilled from young and mature leaves of Artemisia scoparia. GC-MS analyses revealed a monoterpenoid nature (64-67%) with 44 and 31 constituents in young and mature leaves oil, respectively. The oil from young leaf contained greater amount of oxygenated compounds. Beta-myrcene (24.13%) and p-cymene (27.06%) were the major constituents in young and mature leaves oil, respectively. A. scoparia leaf oils (25-200 microg/ml) exhibited a strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity and antioxidant activity against hydroxyl radical and hydrogen peroxide. However, the activities of major constituent monoterpenes, beta-myrcene and p-cymene, were less. In general, the DPPH radical scavenging and antioxidant activity was in the order: mature leaf oil > young leaf oil > beta-myrcene > p-cymene.
authors.affiliation: Department of Environment and Vocational Studies, Panjab University, Chandigarh 160 014, India. hpsingh_01@yahoo.com
authors.forename: Harminder Pal
authors.initials: HP
authors.lastname: Singh
authors.forename: Shalinder
authors.initials: S
authors.lastname: Kaur
authors.forename: Sunil
authors.initials: S
authors.lastname: Mittal
authors.forename: Daizy R
authors.initials: DR
authors.lastname: Batish
authors.forename: Ravinder K
authors.initials: RK
authors.lastname: Kohli
mesh_terms: D000375:Aging / Q000502:physiology; D019306:Artemisia / Q000737:chemistry* / Q000254:growth & development; D002734:Chlorophyll / Q000032:analysis; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D010938:Plant Oils; D010940:Plant Proteins; D017392:Thiobarbituric Acid Reactive Substances; D002734:Chlorophyll; D006861:Hydrogen Peroxide