title: Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway.
abstract: BACKGROUND
  Norwegian production of rainbow trout (Oncorhynchus mykiss) has been without any outbreaks of VHS for many years until the disease emerged in a farm in western Norway in November 2007. The fish were, in addition to VHS virus, positive for gill chlamydia-like bacteria, Flavobacterium psychrophilum, and a microsporidian. A new VHS virus genotype III was isolated from the fish in RTgill-W1 cells and the complete coding region (11,065 nucleotides) was sequenced. This virus was also used in a challenge experiment to see if it could cause any mortality in rainbow trout in sea water.
  
  
  RESULTS
  This is the first time a nearly complete sequence of a genotype III virus isolate has been presented. The organization of the genes is the same as in the other VHS virus genotypes studied (GI and GIV). Between the ORFs are nontranslated regions that contain highly conserved sequences encompassing the polyadenylation signal for one gene, and the putative transcription initiation site of the next gene. The intergenic regions vary in length from 74 nt to 128 nt. The nucleotide sequence is more similar to genotype I isolates compared to isolates from genotype II and IV. Analyses of the sequences of the N and G protein genes show that this new isolate is distinct from other VHS virus isolates and groups closely together with isolates from genotype III. In a challenge experiment, using intraperitoneal (ip) injection of the isolate, co-habitation with infected fish, and bath challenge, mortalities slightly above 40% were obtained. There was no significant difference in mortality between the bath challenged group and the ip injected group, while the mortality in the co-habitation group was as low as 30%.
  
  
  CONCLUSIONS
  All VHS virus isolates in genotype III are from marine fish in the North East Atlantic. Unlike the other known genotype III isolates, which are of low virulence, this new isolate is moderately virulent. It was not possible to detect any changes in the virus genome that could explain the higher virulence. A major problem for the study of virulence factors is the lack of information about other genotype III isolates.
authors.affiliation: Department of Biology, University of Bergen, Thormohlensgt 55, 5020 Bergen, Norway.
authors.forename: Henrik
authors.initials: H
authors.lastname: Duesund
authors.forename: Stian
authors.initials: S
authors.lastname: Nylund
authors.forename: Kuninori
authors.initials: K
authors.lastname: Watanabe
authors.forename: Karl F
authors.initials: KF
authors.lastname: Ottem
authors.forename: Are
authors.initials: A
authors.lastname: Nylund
mesh_terms: D000818:Animals; D017756:Aquaculture; D016000:Cluster Analysis; D021901:DNA, Intergenic; D023061:Gene Order; D005838:Genotype; D031941:Hemorrhagic Septicemia, Viral / Q000401:mortality / Q000821:virology*; D008969:Molecular Sequence Data; D009664:Norway; D029141:Novirhabdovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017686:Oncorhynchus mykiss / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016019:Survival Analysis; D026801:Synteny; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
chemical_list: D021901:DNA, Intergenic; D012367:RNA, Viral; D014764:Viral Proteins