Expression and functional characterization of tumor-targeted fusion protein composed of NGR peptide and 15-kDa actin fragment.
To induce tumor cell apoptosis, a modified 15 kDa actin linked with a peptide NGR "homing" into tumor or tumor vessels was expressed in Escherichia coli. After refolding and purification, this fusion protein NGR-15actin was labeled with FITC to testify whether NGR-15actin could integrate into the cytoskeleton. It was found that this targeted peptide could induce HepG2 and HeLa cells apoptosis through its effect on the cytoskeleton function by binding to cytoskeleton protein. Thus, targeted NGR-15actin could be a candidate molecule for the therapy of cancer.
Lei
Huanzong
H
Department of Biology, School of Chemistry and Life Sciences, Lishui University, Lishui, 323000 Zhejiang, People's Republic of China.
Cao
Peng
P
Miao
Guoping
G
Lin
Zhihua
Z
Diao
Zhenyu
Z
D000199:Actins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000503:physiopathology; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
D000199:Actins; C526428:NGR peptide; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins