title: Regulation of cellular actin architecture by S100A10.
abstract: Actin structures are involved in several biological processes and the disruption of actin polymerisation induces impaired motility of eukaryotic cells. Different factors are involved in regulation and maintenance of the cytoskeletal actin architecture. Here we show that S100A10 participates in the particular organisation of actin filaments. Down-regulation of S100A10 by specific siRNA triggered a disorganisation of filamentous actin structures without a reduction of the total cellular actin concentration. In contrast, the formation of cytoskeleton structures containing tubulin was unhindered in S100A10 depleted cells. Interestingly, the cellular distribution of annexin A2, an interaction partner of S100A10, was unaffected in S100A10 depleted cells. Cells lacking S100A10 showed an impaired migration activity and were unable to close a scratched wound. Our data provide first insights of S100A10 function as a regulator of the filamentous actin network.
authors.affiliation: Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, University Hospital Jena, Jena, Germany.
authors.forename: M Juliane
authors.initials: MJ
authors.lastname: Jung
authors.forename: Ulrike
authors.initials: U
authors.lastname: Murzik
authors.forename: Liane
authors.initials: L
authors.lastname: Wehder
authors.forename: Peter
authors.initials: P
authors.lastname: Hemmerich
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D002477:Cells / Q000378:metabolism / Q000648:ultrastructure; D003599:Cytoskeleton / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology; D009418:S100 Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D000099248:S100 Calcium Binding Protein A10
chemical_list: D000199:Actins; D017306:Annexin A2; D034741:RNA, Small Interfering; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10