title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci.
abstract: Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered from 22 Spanish localities during a nine-year period (1998-2007). The combination of five loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32 and VNTR-259) enabled the differentiation of 12 allelic profiles, with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.84. Moreover, we obtained MIRU-VNTR patterns that were unique for each of the M. a. paratuberculosis types analyzed (II and III); other patterns were host-related or restricted to geographic areas. Therefore, this MIRU-VNTR approach could be a useful sub-typing molecular tool in order to get a better sense of the epidemiology of Johne's disease.
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria VISAVET, Universidad Complutense de Madrid, Avda Puerta de Hierro, 28040 Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Castellanos
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rodríguez
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.forename: Ana
authors.initials: A
authors.lastname: Mateos
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005798:Genes, Bacterial; D018598:Minisatellite Repeats / Q000235:genetics*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012756:Sheep / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial