Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish, Fistularia commersonii.
A Gram-stain-negative, non-motile, non-spore-forming and short rod- or rod-shaped bacterial strain, designated 22-5(T), was isolated from a bluespotted cornetfish, Fistularia commersonii, and subjected to taxonomic study. Strain 22-5(T) grew optimally at 30&emsp14;°C and in the presence of 2-5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 22-5(T) belonged to the genus Paracoccus and joined the cluster comprising Paracoccus homiensis DD-R11(T) and Paracoccus zeaxanthinifaciens ATCC 21588(T), with which strain 22-5(T) exhibited 97.4 and 96.9 % 16S rRNA gene sequence similarity, respectively. Strain 22-5(T) exhibited 94.0-96.6 % 16S rRNA gene sequence similarity with the other type strains of species of the genus Paracoccus. Strain 22-5(T) contained Q-10 as the predominant menaquinone and C(18 : 1)ω7c as the predominant fatty acid. In this study, P. zeaxanthinifaciens KCTC 22688(T) also contained Q-10 as the predominant isoprenoid quinone. The DNA G+C content of strain 22-5(T) was 63.6 mol%. Strain 22-5(T) exhibited 44 and 32 % DNA-DNA relatedness to P. homiensis KACC 11518(T) and P. zeaxanthinifaciens KCTC 22688(T), respectively. On the basis of phenotypic, phylogenetic and genetic data, strain 22-5(T) is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccus fistulariae sp. nov. is proposed. The type strain is 22-5(T) (=KCTC 22803(T) =CCUG 58401(T)).
Kim
Young-Ok
YO
Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 619-705, Republic of Korea.
Kong
Hee Jeong
HJ
Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 619-705, Republic of Korea.
Park
Sooyeon
S
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Kim
Kyung-Kil
KK
Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 619-705, Republic of Korea.
Moon
Dae Yeon
DY
Fisheries Resources Research Division, National Fisheries Research and Development Institute, Gijang, Busan 619-705, Republic of Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2