Evaluation of evidenced-based radioprotective efficacy of Gymnema sylvestre leaves in mice brain.
A Gymnema sylvestre leaves extract (GSE) rich in gymnemic acids was examined for its antioxidant activities through various in vitro assays, along with its radioprotective efficacy in mice brain. The IC(50) values of GSE for 2,2-diphenyl-1-picryl-hydrazyl scavenging assays, superoxide radical scavenging assays, inhibition of in vitro lipid peroxidation assays, and protein carbonyl formation assay were 238, 140, 99.46, and 28.03 microg/mL, respectively. Furthermore, the total phenolic content in GSE was equivalent to 18.06 microg/mL of Gallic acid. The rate of *OH radical scavenging activity of GSE is 0.46 times slower than SCN- derived from nanosecond pulse radiolysis studies. Results of in vivo studies showed that radiation (8 Gy)-induced augmentation in the levels of lipid peroxidation and depletion in glutathione and protein levels in mice brain were significantly ameliorated by GSE pretreatment. Results suggest that the radioprotective efficacy of GSE may be due to its antioxidant properties.
Sharma
K
K
Radiation Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, Rajasthan 302055. sharma.krishna.v@gmail.com
Singh
Umang
U
Vats
Sharad
S
Priyadarsini
K
K
Bhatia
A
A
Kamal
Raka
R
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001930:Brain Injuries / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D005978:Glutathione / Q000378:metabolism; D031104:Gymnema sylvestre / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D005978:Glutathione