MiRNA-26b regulates the expression of cyclooxygenase-2 in desferrioxamine-treated CNE cells.
Here we report that miR-26b is involved in COX-2 overexpression in desferrioxamine (DFOM)-treated carcinoma of nasopharyngeal epithelial (CNE) cells. The level of miR-26b in DFOM-treated CNE cells is inversely proportional to the expression level of the COX-2 protein. Overexpression of miR-26b in DFOM-treated CNE cells inhibits cell proliferation. A luciferase reporter gene experiment suggests that the 3' untranslated region of COX-2 carries a binding site for miR-26b. Overexpression of miR-26b marginally reduces the levels of COX-2 protein in DFOM-treated CNE cells. Moreover, knockdown of COX-2 expression had a similar effect to overexpression of miR-26b. Taken together, these results suggest that miR-26b regulates COX-2 expression in DFOM-treated cells.
Ji
Yanhong
Y
MOE Key laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. jiyh@scnu.edu.cn
He
Yonghong
Y
Liu
Le
L
Chong
Xinyuan
X
Zhong
Xingyuan
X
D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017262:Siderophores / Q000494:pharmacology
D035683:MicroRNAs; D017262:Siderophores; D051546:Cyclooxygenase 2; D003676:Deferoxamine