Assay systems for measurement of anti-inflammatory activity.
It is widely accepted that cationic antimicrobial peptides possess potent microbicidal properties. Recent studies show that in addition to their antimicrobial action, these peptides can exhibit anti-inflammatory activity. The purpose of this chapter is to describe in vivo ear inflammation models that can be used for evaluating the anti-inflammatory activity of antimicrobial peptides. The models are based on different mechanisms of inflammation development and include irritant dermatitis (a model induced by a single application of 12-o-tetradecanoylphorbol acetate [TPA]) and allergic dermatitis, or delayed type hypersensitivity reaction (a model induced by repetitive application of oxazolone).
Rubinchik
Evelina
E
MIGENIX Inc., Vancouver, BC, Canada.
Pasetka
Christopher
C
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000276:immunology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000276:immunology* / Q000627:therapeutic use; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy / Q000276:immunology; D017453:Dermatitis, Irritant / Q000188:drug therapy / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D051379:Mice; D010081:Oxazolone; D013755:Tetradecanoylphorbol Acetate
D000893:Anti-Inflammatory Agents; D023181:Antimicrobial Cationic Peptides; D010081:Oxazolone; D013755:Tetradecanoylphorbol Acetate