Spectrofluorimetric determination of folic acid in tablets and urine samples using 1,10-phenanthroline-terbium probe.
A new spectrofluorimetric method was reported for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of Tb(3+) -1,10-phenanthroline complex as a fluorescent probe. The quenched fluorescence intensity at an emission wavelength of 545 nm was proportional to the concentration of FA in Tris-HCl buffer solution of pH 6.2. The effects of pH, time, order of addition of reagents, temperature and concentrations of Tb(3+) , buffer and 1,10-phenanthroline were investigated and optimized. The linear range for the determination of FA was 0.01-1.1 mg/L. The detection limit was 0.003 mg/L and the relative standard deviation for replicated determination of 1 mg/L of folic acid was 1.2%. This method was simple, practical and relatively free from interference effects. It was successfully applied to assess FA in pharmaceutical tablets and urine samples.
Manzoori
Jamshid L
JL
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. manzoori@tabrizu.ac.ir
Jouyban
Abolghasem
A
Amjadi
Mohammad
M
Soleymani
Jafar
J
D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D005492:Folic Acid / Q000032:analysis* / Q000652:urine*; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D013725:Terbium / Q000737:chemistry*
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010618:Phenanthrolines; D013607:Tablets; D013725:Terbium; D005492:Folic Acid; C025205:1,10-phenanthroline