title: Preliminary crystallographic characterization of the Grb2 SH2 domain in complex with a FAK-derived phosphotyrosyl peptide.
abstract: Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein with a single SH2 domain that specifically binds to focal adhesion kinase (FAK) when residue Tyr925 of FAK is phosphorylated. The Grb2-FAK interaction is associated with cellular integrin-activated signal transduction events leading to the activation of the Ras-MAPK pathway. Crystals of the Grb2 SH2 domain in complex with a phosphopeptide corresponding to residues 921-930 of FAK have been obtained using the sitting-drop vapour-diffusion technique. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 102.7, c = 127.6 A, alpha = beta = 90.0, gamma = 120.0 degrees . A diffraction data set was collected from a flash-cooled crystal at 100 K to 2.49 A resolution using synchrotron radiation. Structure determination by molecular replacement and analysis of the detailed structure of the complex are currently in progress.
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsiao Hsin
authors.initials: HH
authors.lastname: Chen
authors.forename: Cuei Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Yu Yung
authors.initials: YY
authors.lastname: Chang
authors.forename: Tang Long
authors.initials: TL
authors.lastname: Shen
authors.forename: Chun Hua
authors.initials: CH
authors.lastname: Hsu
mesh_terms: D003461:Crystallography; D051417:Focal Adhesion Kinase 1 / Q000737:chemistry* / Q000378:metabolism; D051380:GRB2 Adaptor Protein / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D014961:X-Ray Diffraction; D018909:src Homology Domains
chemical_list: D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D010455:Peptides; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human