title: WellReader: a MATLAB program for the analysis of fluorescence and luminescence reporter gene data.
abstract: BACKGROUND
  Fluorescent and luminescent reporter gene systems in combination with automated microplate readers allow real-time monitoring of gene expression on the population level at high precision and sampling density. This generates large amounts of data for the analysis of which computer tools are missing to date.
  
  
  RESULTS
  We have developed WellReader, a MATLAB program for the analysis of fluorescent and luminescent reporter gene data. WellReader allows the user to load the output files of microplate readers, remove outliers, correct for background effects and smooth and fit the data. Moreover, it computes biologically relevant quantities from the measured signals, notably promoter activities and protein concentrations, and compares the resulting expression profiles of different genes under different conditions.
  
  
  BACKGROUND
  WellReader is available under a LGPL licence at http://prabi1.inrialpes.fr/trac/wellreader.
authors.affiliation: INRIA Grenoble-Rhône-Alpes, Saint Ismier Cedex, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Boyer
authors.forename: Bruno
authors.initials: B
authors.lastname: Besson
authors.forename: Guillaume
authors.initials: G
authors.lastname: Baptist
authors.forename: Jérôme
authors.initials: J
authors.lastname: Izard
authors.forename: Corinne
authors.initials: C
authors.lastname: Pinel
authors.forename: Delphine
authors.initials: D
authors.lastname: Ropers
authors.forename: Johannes
authors.initials: J
authors.lastname: Geiselmann
authors.forename: Hidde
authors.initials: H
authors.lastname: de Jong
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D003196:Computer Graphics; D005456:Fluorescent Dyes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049449:Luminescence; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011381:Programming Languages; D012984:Software
chemical_list: D005456:Fluorescent Dyes