[Protective effect of N-acetylcysteine on liver and lung in mice after ischemia-reperfusion injury].
OBJECTIVE
To investigate protective effect of N-acetylcysteine (NAC) on liver and lung in mice after hepatic ischemia/reperfusion injury.


METHODS
BALB/s mice were used in a model of partial hepatic ischemia/reperfusion (I/R) injury. They are divided randomly to sham-operated control group (SH), hepatic I/R group or NAC pretreated in hepatic I/R group (I/R-NAC). The level of TNF-alpha in portal vein and plasma ALT were measured at 1 hour and 3 hour, respectively after reperfusion. Lung tissue wet-to-dry (W/D) weight ratio compared.


RESULTS
Lung tissue W/D ratio showed significant difference between two groups; The expressions of TLR2/4 mRNA in liver and lung increased obviously after hepatic I/R injury. Histological evaluation showed several changes in lung tissue in I/R group. The level of TNF-alpha and ALT declined significantly in the group pretreated by NAC.


CONCLUSIONS
N-acetylcysteine can inhibit the activation of TLR2/4 and reduce TNF-alpha secretion resulted from I/R injury it might abate liver and lung injury following partial hepatic ischemia-reperfusion in mice.
Zeng
Shan
S
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China.
Lin
Yi
Y
Di
Jing-fang
JF
Feng
Zheng
Z
D000111:Acetylcysteine / Q000494:pharmacology* / Q000627:therapeutic use; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D051195:Toll-Like Receptor 2 / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D012333:RNA, Messenger; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase; D000111:Acetylcysteine