Characterization of non-endcapped polymeric ODS column for the separation of triacylglycerol positional isomers.
The characteristics of a non-endcapped polymeric ODS column for the resolution of triacylglycerol positional isomers (TAG-PI) were examined using a recycle HPLC-atmospheric pressure chemical ionization/mass spectrometry system. A pair of TAG-PI containing saturated fatty acids at least 12 carbons was separated. Except for TAG-PI containing elaidic acid, pairs of TAG-PI containing three unsaturated fatty acids were not separated, even by recycle runs. These results indicate that the resolution of TAG-PI on a non-endcapped polymeric ODS stationary phase is realized by the recognition of the linear structure of the fatty acid and the binding position of the saturated fatty acid in TAG-PI. Chain length was also an important factor for resolution. This method may be a useful and simple for measuring the abundance ratio of TAG-PI containing saturated fatty acids in natural oils.
Gotoh
Naohiro
N
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Matsumoto
Yumiko
Y
Yuji
Hiromi
H
Nagai
Toshiharu
T
Mizobe
Hoyo
H
Ichioka
Kenji
K
Kuroda
Ikuma
I
Noguchi
Noriko
N
Wada
Shun
S
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007536:Isomerism; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification*
D011108:Polymers; D014280:Triglycerides; C026679:octadecylsilica; D012822:Silicon Dioxide