Generation of monoclonal antibodies against human regulatory T cells.
Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.
Becker
Christian
C
Department of Dermatology of University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse1, 55101 Mainz, Germany. christian.becker@hautklinik.klinik.uni-mainz.de
Hoschützky
Heinz
H
Rist
Wolfgang
W
Lenter
Martin
M
Schneider
Franz-Joseph
FJ
Jonuleit
Helmut
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D002469:Cell Separation / Q000379:methods; D018604:Epitope Mapping; D000939:Epitopes; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D006825:Hybridomas; D007115:Immunization Schedule; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D007937:Leukapheresis; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D011948:Receptors, Antigen, T-Cell