A preliminary X-ray study of D,D-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis E264.
D,D-Heptose-1,7-bisphosphate phosphatase (GmhB), which is involved in the third step of the NDP-heptose biosynthesis pathway, converts D,D-heptose-1,7-bisphosphate to D,D-heptose-1-phosphate. This biosynthesis pathway is a target for new antibiotics or antibiotic adjuvants for Gram-negative pathogens. Burkholderia thailandensis is a useful surrogate organism for studying the pathogenicity of melioidosis owing to its extensive genomic similarity to B. pseudomallei. Melioidosis caused by B. pseudomallei is a serious invasive disease of animals and humans in tropical and subtropical areas. In this study, GmhB has been cloned, expressed, purified and crystallized. X-ray data have also been collected to 2.50 A resolution using synchrotron radiation. The crystal belonged to space group P6, with unit-cell parameters a = 243.2, b = 243.2, c = 41.1 A.
Kim
Mi Sun
MS
College of Pharmacy, Division of Life and Pharmaceutical Sciences and Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Seoul 120-750, Republic of Korea.
Shin
Dong Hae
DH
D019117:Burkholderia / Q000201:enzymology*; D018360:Crystallography, X-Ray; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*
D010744:Phosphoric Monoester Hydrolases