[Effects of diethylstilbestrol on cultured testis gubernacular cells in mice].
OBJECTIVE
To establish a primary culture of the testis gubernacular cells of Kunming mice, observe the morphological characteristics of the cells, and explore the effects of exogenous estrogens (EEs) on the development of the testis gubernacula in vitro.


METHODS
We removed the gubernacula from 3-day-old mice with the surgical magnifier and cultured the gubernacular cells. Then we detected the cell viability by trypan blue and cell morphology by HE staining. The subcultured cells were randomly divided into a blank control, a DMSO (0.1%, v/v) control, and 4 experimental groups (given 0.01, 0.10, 1.00 and 10.00 micdrog/ml of diethylstilbestrol [DES] dissolved in DMSO, respectively). After treated for 12, 24 and 48 hours, the gubernacular cells were observed for morphological changes and proliferation inhibition by CCK-8.


RESULTS
Most of the cultured gubernacular cells were fibroblasts, and a few were epithelioids. The primary cells showed a viability of 85%-90%. Dose- and time-dependent inhibition of cell proliferation was found in the four experimental groups at three different times, with statistically significant differences (P < 0.01).


CONCLUSIONS
Gubernacular cells can be cultured in vitro. EEs inhibit the proliferation of gubernacular cells in a dose- and time-dependent manner. An in- sight into the effects EES on cultured gubernacular cells is an effective approach to the study of their influence on the development of the reproductive system.
Zhang
Xuan
X
Department of Pediatric Surgery, The Second Hospital, China. zhxuan2006@yahoo.com.cn
Jiang
Xue-wu
XW
Li
Jian-hong
JH
Ma
Lian
L
Huang
Tian-hua
TH
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013085:Spermatic Cord / Q000166:cytology / Q000187:drug effects; D013737:Testis / Q000166:cytology* / Q000187:drug effects*
D004968:Estrogens, Non-Steroidal; D004054:Diethylstilbestrol