title: Comparative evaluation of the erbB2 and hormone receptor status of neighboring invasive and in situ components of ductal carcinomas of the breast.
abstract: BACKGROUND
  It remains unknown whether erbB2 expression and hormone receptor status predict the invasive potential of ductal carcinoma in situ (DCIS) of the breast.
  
  
  OBJECTIVE
  To examine erbB2 and estrogen/progesterone receptor (ER/PR) status in the precise areas where DCIS turns into invasive ductal carcinoma (IDC).
  
  
  METHODS
  Eighty-seven cases of breast malignancies harboring contiguous regions of DCIS and IDC were selected. Separate histological samples from the DCIS and the neighboring IDC were obtained using tissue microarrays. The erbB2 and ER/PR statuses were assessed using immunohistochemistry (erbB2 and ER/PR) and fluorescence in situ hybridization (FISH - only erbB2).
  
  
  RESULTS
  The expression of erbB2 did not differ in the DCIS and IDC components of the breast tumors (p=0.35). There was good agreement in sample-by-sample comparisons of erbB2 (intraclass correlation coefficient [ICC]=0.78), PR (ICC=0.61) and ER (ICC=0.70) expression in the DCIS and IDC components.
  
  
  CONCLUSIONS
  Our findings suggest that the expressions of erbB2 and ER/PR do not differ in the contiguous regions from DCIS to IDC.
authors.affiliation: CAISM Women's Hospital, University of Campinas UNICAMP, Campinas, Brazil.
authors.forename: Raquel Mary Rodrigues
authors.initials: RM
authors.lastname: Peres
authors.forename: Kátia Piton
authors.initials: KP
authors.lastname: Serra
authors.forename: Sophie F M
authors.initials: SF
authors.lastname: Derchain
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Glauce Aperecida
authors.initials: GA
authors.lastname: Pinto
authors.forename: Marcelo
authors.initials: M
authors.lastname: Alvarenga
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Soares
authors.forename: Juliana K
authors.initials: JK
authors.lastname: Heinrich
authors.forename: Isabela Werneck
authors.initials: IW
authors.lastname: da Cunha
authors.forename: Jose
authors.initials: J
authors.lastname: Vassallo
authors.forename: Luís Otávio
authors.initials: LO
authors.lastname: Sarian
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human