title: Comparison of the molecular influences of NO-induced lesions in DNA strands on the reactivity of polynucleotide kinases, DNA ligases and DNA polymerases.
abstract: Nitric oxide (NO) causes DNA damage, generating xanthine (Xan, X) and oxanine (Oxa, O) from guanine (Gua, G) and hypoxanthine (Hyp, H) from adenine (Ade, A) by nitrosative oxidation. Although these NO-induced lesions have been thought to cause mutagenic problems in cellular systems, the influence of these lesions on enzymatic functions has not yet been compared systematically. In this study, we investigated the effect of NO-induced lesions on the activities of DNA-binding/recognizing enzymes such as T4 polynucleotide kinase (T4 PNK), DNA ligases (T4 DNA ligase, Taq DNA ligase) and DNA polymerases (E. coli DNA polymerase I, Klenow fragment, T4 DNA polymerase). The phosphorylation efficiencies of T4 PNK are dependent on the base type at the 5'-end of single-stranded DNA, where Oxa congruent with Hyp congruent with Gua > Xan congruent with Ade. The enzymatic reactions efficiencies of DNA ligases or DNA polymerases were observed to be dependent on the base-pairing type bound by the enzymes, where G:C > H:C > O:C > X:C and A:T congruent with H:T > O:T > X:T. These results suggested that NO-induced lesions and their base-pairs could participate in the interaction mechanisms of the DNA-binding/recognizing enzymes in a similar manner as natural nucleobases.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Doi
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D011088:DNA Ligases / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D009569:Nitric Oxide / Q000494:pharmacology*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases