title: DNA zip codes control an ancient mechanism for gene targeting to the nuclear periphery.
abstract: Many genes in Saccharomyces cerevisiae are recruited to the nuclear periphery after transcriptional activation. We have identified two gene recruitment sequences (GRS I and II) from the promoter of the INO1 gene that target the gene to the nuclear periphery. These GRSs function as DNA zip codes and are sufficient to target a nucleoplasmic locus to the nuclear periphery. Targeting requires components of the nuclear pore complex (NPC) and a GRS is sufficient to confer a physical interaction with the NPC. GRS I elements are enriched in promoters of genes that interact with the NPC, and genes that are induced by protein folding stress. Full transcriptional activation of INO1 and another GRS-containing gene requires GRS-mediated targeting of the promoter to the nuclear periphery. Finally, GRS I also functions as a DNA zip code in Schizosaccharomyces pombe, suggesting that this mechanism of targeting to the nuclear periphery has been conserved over approximately one billion years of evolution.
authors.affiliation: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Ahmed
authors.forename: Donna G
authors.initials: DG
authors.lastname: Brickner
authors.forename: William H
authors.initials: WH
authors.lastname: Light
authors.forename: Ivelisse
authors.initials: I
authors.lastname: Cajigas
authors.forename: Michele
authors.initials: M
authors.lastname: McDonough
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Froyshteter
authors.forename: Tom
authors.initials: T
authors.lastname: Volpe
authors.forename: Jason H
authors.initials: JH
authors.lastname: Brickner
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D016681:Genome, Fungal / Q000235:genetics; D008954:Models, Biological; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics; D022022:Nuclear Pore / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D012568:Schizosaccharomyces / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C519641:INO1 protein, S cerevisiae; D007296:Myo-Inositol-1-Phosphate Synthase