title: The disinfection efficacy of MeniCare soft multipurpose solution against Acanthamoeba and viruses using stand-alone biocidal and regimen testing.
abstract: OBJECTIVE
  To assess the disinfection efficacy of MeniCare Soft contact lens multipurpose solution against Acanthamoeba and viruses in suspension and when inoculated on to contact lenses and subjected to rub-and-rinse or no-rub-and-rinse care regimes.
  
  
  METHODS
  MeniCare Soft was challenged with Acanthamoeba spp trophozoites or cysts, herpes simplex virus (type 1), adenovirus (type 8), and poliovirus (type 2) and the log reduction in Acanthamoeba viability and viral infectivity determined over time. In addition, contact lenses were incubated with Acanthamoeba and viruses and the number of viable organisms determined after the lenses were processed using rub-and-rinse or no-rub-and-rinse care regimes followed by a 4 hr soaking time.
  
  
  RESULTS
  MeniCare Soft showed >3 log reduction against Acanthamoeba spp trophozoites and cysts after 6 hr exposure. Approximately 1 log reduction was found against the 3 viruses after 4 hr exposure. No surviving Acanthamoeba trophozoites or cysts were recovered from any of the contact lens tested when MeniCare Soft was used in a rub-and-rinse or no-rub-and-rinse care regimes (>5.0 log reduction). Rub-and-rinse regimen resulted in a 4.5 to 5.0 log reduction in viruses compared with 3.7 to 5.2 log when no-rub-and-rinse was used.
  
  
  CONCLUSIONS
  MeniCare Soft showed effective disinfection efficacy against Acanthamoeba trophozoites and cysts using solution and regimen assays. The viruses were more resistant to disinfection in solution but were removed effectively from contact lenses using a rub-and-rinse or no-rub-and-rinse care regimen.
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
authors.forename: Wayne
authors.initials: W
authors.lastname: Heaselgrave
authors.forename: James
authors.initials: J
authors.lastname: Lonnen
authors.forename: Simon
authors.initials: S
authors.lastname: Kilvington
authors.forename: Jacinto
authors.initials: J
authors.lastname: Santodomingo-Rubido
authors.forename: Osamu
authors.initials: O
authors.lastname: Mori
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects* / Q000254:growth & development; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology* / Q000469:parasitology*; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods* / Q000592:standards; D006801:Humans; D013997:Time Factors; D053058:Trophozoites / Q000187:drug effects; D014780:Viruses / Q000187:drug effects*
chemical_list: D018639:Contact Lens Solutions; D004202:Disinfectants