Gastritis induced by Helicobacter pylori infection in experimental rats.
BACKGROUND
Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide.


OBJECTIVE
Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination.


METHODS
Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-year-old male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E(2) (PGE(2)) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis.


RESULTS
Serum gastrin, IL-6, mucosal MPO activity, and PGE(2) demonstrated significant increases joined with a decreased serum pepsinogen I activity (P < 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation.


CONCLUSIONS
Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.
Elseweidy
Mohamed M
MM
Department of Biochemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt. mmelseweidy@yahoo.com
Taha
Mona M
MM
Younis
Nahla N
NN
Ibrahim
Khadiga S
KS
Hamouda
Hamdi A
HA
Eldosouky
Mohamed A
MA
Soliman
Hala
H
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D053938:DNA Fragmentation; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005755:Gastrins / Q000378:metabolism; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism* / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D020116:Pepsinogen A / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011706:Pyloric Antrum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D005755:Gastrins; D015850:Interleukin-6; D020116:Pepsinogen A; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D015232:Dinoprostone