Determination of coenzyme A (CoASH) in the presence of different thiols by using flow-injection with a UV/Vis spectrophotometric detector and potentiometric determination of CoASH using an iodide ISE.
Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of CoASH is needed. Here we are described a simple and fast way of determinating CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide concentration range (1 x 10(-6)-1 x 10(-4) M) of CoASH.
Giljanović
Josipa
J
Department of Analytical Chemistry, Faculty of Chemistry and Chemical Technology, Teslina 10/V, 21000 Split, Croatia. josipa@ktf-split.hr
Prkić
Ante
A
D003065:Coenzyme A / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D007454:Iodides / Q000737:chemistry*; D017736:Ion-Selective Electrodes; D007700:Kinetics; D011199:Potentiometry / Q000295:instrumentation*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D007454:Iodides; D013438:Sulfhydryl Compounds; D003065:Coenzyme A