Detection of G1 genotype of human cystic echinococcosis in Egypt.
The first trial to detect G1 genotype in Egyptian human isolates of hydatid cysts (HC) and serum samples to approach diagnosis of cystic echinococcosis (CE) using human sera by PCR. Using strain specific primers, 27/36 confirmed CE patients (75%) showed G1 specific band in their sera at 254 bp. Specificity was 100% without detecting bands for either other parasitosis, or mass occupying lesions. Using PCR, G1 genotype was detected in 83.3% of HC samples, without significant difference between types of human isolates (pulmonary, hepatic, or multi-organ). G1 genotype detection in human sera was in 75% of CE patients compared to 83.3% in HC samples of the same group of patients proved satisfactory, simple and safer than HCF sampling. IHAT gave sensitivity of 58.3% compared to histopathological examination of surgically removed cysts or examination of hydatid cyst fluid (HCF) for protoscolices (gold standards). The specificity was 70% with false positive reactions with other parasitic infections and mass occupying lesions. PCR detection of G1 genotype in Egyptian animal hydatid cysts showed 90% in camel isolates and 80% in sheep isolates, but pig isolates were negative. The presence of this genotype in a high percentage in camel isolates incriminated sheep strain as the source of CE camel infection. The results may give an explanation to the contradicting results of other studies that did not relay upon molecular aspects.
Abd El Baki
Mohammad H
MH
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
El Missiry
Adel M G
AM
Abd El Aaty
Heba E M
HE
Mohamad
Anhar A
AA
Aminou
Heba A R
HA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002162:Camelus / Q000469:parasitology; D002648:Child; D018509:DNA, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000175:diagnosis / Q000469:parasitology*; D004446:Echinococcus / Q000235:genetics / Q000302:isolation & purification*; D004534:Egypt; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000592:standards*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D012756:Sheep; D055815:Young Adult
D018509:DNA, Helminth