Impact of ERCC2 gene polymorphism on HIV-1 disease progression to AIDS among North Indian HIV patients.
HIV/AIDS remains to be one of the killing diseases of mankind. Host genetic response is one of the factor which determine susceptibility to HIV and disease progression to AIDS. The aim of the present study was to evaluate the impact of ERCC2 Lyc ( 751 ) Gln (excision repair cross complementing rodent repair deficiency, complementation group 2) polymorphism on HIV-1 susceptibility and disease progression to AIDS, as this gene has been reported to intervene in degrading retroviral cDNA before it integrates with the host DNA. This case control study included 300 HIV seropositive cases and an equal number of HIV seronegative controls. DNA was isolated from the blood samples of study subjects and genotyping of ERCC2 was conducted by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. The Gln/Gln genotype showed a significant variation between cases and controls (P = 0.047, OR 1.71, 95% CI 1.00-2.93), indicating a possible role of susceptibility in reference to controls and disease progression when compared within cases.
Sobti
Ranbir Chander
RC
Department of Biotechnology, Panjab University, Chandigarh, Chandigarh 16 00 14, India. rcsobti@pu.ac.in
Berhane
Nega
N
Mahdi
Salih Abdul
SA
Kler
Rupinder
R
Hosseini
Seyed Ali
SA
Kuttiat
Vijish
V
Wanchu
Ajay
A
D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology*; D000293:Adolescent; D000328:Adult; D018450:Disease Progression; D005260:Female; D020022:Genetic Predisposition to Disease; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology*; D015497:HIV-1; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*; D055815:Young Adult
D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human