Increased levels of resistin in rotating shift workers: a potential mediator of cardiovascular risk associated with circadian misalignment.
OBJECTIVE
Shift work schedule has been associated with several health problems, including deleterious effects on the cardiovascular system. The present study aimed to evaluate the circulating levels of four biomarkers of atherosclerosis (soluble CD40 ligand [sCD40L], monocyte chemoattractant protein-1 [MCP-1], resistin, and plasminogen activator inhibitor-1 [PAI-1]) in a population-based sample of young adult men exposed to rotating shift work schedule in comparison with day workers.


METHODS
A total of 439 men aged 34.4+/-8.6 years were included in a cross-sectional study comparing 255 day workers with 184 rotating shift workers. Circulating levels of the biomarkers were measured in duplicate by ELISA using monoclonal specific antibodies.


RESULTS
Rotating shift workers had elevated (6440+/-4510 pg/mL) (mean+/-SD) circulating levels of resistin in comparison with day workers (5450+/-3780 pg/mL), and significance remains after adjusting for age and blood leukocyte count (p<0.045, ANCOVA). Shift work schedule explains 1% of the proportion of the total variation in the circulating resistin levels. Multiple regression analysis showed that resistin levels significantly correlate with rotating shift work (p<0.04) and blood leukocyte count (p<0.00003) independently of age, BMI, waist-hip ratio, HOMA, and cardiovascular risk %. Circulating levels of sCD40L, MCP-1, and PAI-1 did not significantly differ between day workers and shift workers.


CONCLUSIONS
Shift work schedule was significantly associated with elevated plasma resistin levels. Resistin, which is probably produced by leukocytes, may play an important role in the pathogenesis of early metabolic syndrome components in young men chronically exposed to circadian misalignment.
Burgueño
Adriana
A
Molecular Genetics and Biology of Complex Diseases Department, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires-National Council of Scientific and Technological Research (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Gemma
Carolina
C
Gianotti
Tomas Fernández
TF
Sookoian
Silvia
S
Pirola
Carlos Jose
CJ
D000328:Adult; D000911:Antibodies, Monoclonal / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood / Q000175:diagnosis; D023201:CD40 Ligand / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood*; D002319:Cardiovascular System; D018932:Chemokine CCL2 / Q000097:blood; D002940:Circadian Rhythm; D003430:Cross-Sectional Studies; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D052243:Resistin / Q000097:blood*; D014940:Work Schedule Tolerance
D000911:Antibodies, Monoclonal; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D017395:Plasminogen Activator Inhibitor 1; D052243:Resistin; C051321:SERPINE1 protein, human; D023201:CD40 Ligand