The qualities of cryopreserved epididymal sperm collected from feline epididymides stored at low temperature.
We observed the influences of low-temperature storage of the feline epididymis on the epididymal semen qualities before and after cryopreservation to identify the optimal duration for low-temperature storage of the epididymis. After excision, the feline epididymis was stored at 4 degrees C for 0-72 hr and then subjected to epididymal sperm collection. When sperm from the refrigerated cauda epididymis were frozen and thawed, there was no significant difference in sperm motility between the 0- and 24-hr low-temperature storage groups, but sperm motility was significantly decreased in the 48-hr storage group. The above findings suggested that low-temperature storage of the epididymis until 24 hr is useful for frozen sperm collected from the feline cauda epididymis.
Toyonaga
Mari
M
Department of Reproduction, Nippon Veterinary and Life Science University, Japan. marimaru@sd5.so-net.ne.jp
Sato
Yuuka
Y
Morita
Miki
M
Watanabe
Masashi
M
Oba
Hiromichi
H
Mizutani
Tatsuji
T
Hori
Tatsuya
T
Tsutsui
Toshihiko
T
D000818:Animals; D002415:Cats; D002470:Cell Survival; D003080:Cold Temperature; D015925:Cryopreservation / Q000662:veterinary*; D056727:Endangered Species; D004822:Epididymis / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013079:Sperm Maturation / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000166:cytology / Q000502:physiology; D013737:Testis / Q000502:physiology