Isolation, characterization, and evaluation of wild isolates of Lactobacillus reuteri from pig feces.
Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2 approximately 10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.
Lee
Deog Yong
DY
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Priority Research Institute and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
Seo
Yeon-Soo
YS
Rayamajhi
Nabin
N
Kang
Mi Lan
ML
Lee
Su In
SI
Yoo
Han Sang
HS
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004798:Enzymes / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D006461:Hemolysis; D007211:Indoles / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010649:Phenylalanine / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids / Q000032:analysis; D019936:Probiotics / Q000008:administration & dosage* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004798:Enzymes; D007211:Indoles; D012336:RNA, Ribosomal, 16S; D010649:Phenylalanine; D000641:Ammonia; C030374:indole