Host-microbe interaction systems biology: lifecycle transcriptomics and comparative genomics.
The use of microarray and comparative genomic technologies for the analysis of host-pathogen interactions has led to a greater understanding of the biological systems involved in infectious disease processes. Transcriptome analysis of intracellular pathogens at single or multiple time points during infection offers insight into the pathogen intracellular lifecycle. Host-pathogen transcriptome analysis in vivo, over time, enables characterization of both the pathogen and the host during the dynamic, multicellular host response. Comparative genomics using hybridization microarray-based comparative whole-genome resequencing or de novo whole-genome sequencing can identify the genetic factors responsible for pathogen evolutionary divergence, emergence, reemergence or the genetic basis for different pathogenic phenotypes. Together, microarray and comparative genomic technologies will continue to advance our understanding of pathogen evolution and assist in combating human infectious disease.
Sturdevant
Daniel E
DE
Genomics Unit, Research Technologies Section, Research Technologies Branch, Rocky Mountain Laboratories, NIH, 904 South 4th Street, Hamilton, MT 59840, USA. dsturdevant@niaid.nih.gov
Virtaneva
Kimmo
K
Martens
Craig
C
Bozinov
Daniel
D
Ogundare
Olajumoke
O
Castro
Nina
N
Kanakabandi
Kishore
K
Beare
Paul A
PA
Omsland
Anders
A
Ohmsland
Anders
A
Carlson
John H
JH
Kennedy
Adam D
AD
Heinzen
Robert A
RA
Celli
Jean
J
Greenberg
David E
DE
DeLeo
Frank R
FR
Porcella
Stephen F
SF
D055028:Comparative Genomic Hybridization / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D049490:Systems Biology / Q000379:methods*