Culture of human oocytes with granulocyte-macrophage colony-stimulating factor has no effect on embryonic chromosomal constitution.
The effect on ploidy rate in donated human oocytes after in-vitro culture with recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF; 2 ng/ml) from fertilization until day 3 was examined in a multicentre, prospective placebo-controlled and double-blinded study including 73 women donating 86 oocytes. The primary endpoint was to investigate the chromosomal constitution of human embryos (fluorescence in-situ hybridization analysis for chromosomes 13, 16, 18, 21, 22, X and Y) cultured with or without GM-CSF. The secondary endpoints were number of top-quality embryos (TQE) and number of normally developed embryos evaluated morphologically on day 3. The cytogenetic analyses demonstrated non-inferiority and therefore the chromosomal constitution of human embryos cultured in vitro in the presence of 2 ng/ml GM-CSF was no worse than the control group cultured without GM-CSF. In-vitro culture of human embryos in the presence of 2 ng/ml GM-CSF resulted in 34.8% (8/23) uniformly normal embryos. Culture without 2 ng/ml GM-CSF resulted in 33.3% (9/27) uniformly normal embryos. A trend towards a higher number of TQE in the test group was observed; however, due to lack of TQE in the control group, this was considered a random finding.
The Fertility Clinic, Braedstrup Hospital, Sygehusvej 20, Braedstrup, Denmark. inge.e.agerholm@horsens.rm.dk
Inge
I
Agerholm
Anne
A
Loft
Finn
F
Hald
Josephine G
JG
Lemmen
Bibi
B
Munding
Pernille D
PD
Sørensen
Søren
S
Ziebe
D000328:Adult; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects / Q000235:genetics*; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009865:Oocytes / Q000166:cytology*; D011003:Ploidies; D011994:Recombinant Proteins; D020554:Sperm Injections, Intracytoplasmic
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor