Extraction and analysis of auxins in plants using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with fluorescence detection.
Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl(3) (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl(3) was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL(-1) and coefficient correlations in the range of 0.9980-0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350108, China.
Qiaomei
Q
Lu
Lihui
L
Chen
Minghua
M
Lu
Guonan
G
Chen
Lan
L
Zhang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007210:Indoleacetic Acids / Q000032:analysis*; D010944:Plants / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007210:Indoleacetic Acids