17beta-Estradiol increases the number of effector memory CD8+ lymphocytes in mice with contact hypersensitivity and among cultured splenocytes.
To determine how estrogen exacerbates allergies, the effects of 17beta-estradiol (E2) on lymphocyte proliferation were investigated. BALB/c mice were ovariectomized, administered 3.2 microg E2, and sensitized with 50 microL 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one. After 7 days, their spleens were excised and flow cytometrically analyzed. The CD8(+)CD45RA(-)CCR7(-) cell-to-CD8(+) cell ratio in the spleen was greater in the E2-administered mice than in the controls. Splenocytes were cultured under concanavalin A stimulation, with or without E2. After 4 days, the above ratio was greater in the case of E2-treated splenocytes. E2 increases the number of effector memory CD8(+) lymphocytes during sensitization in contact hypersensitivity.
Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan. sakazaki@pharm.setsunan.ac.jp
Fumitoshi
F
Sakazaki
Hitoshi
H
Ueno
Katsuhiko
K
Nakamuro
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000276:immunology*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D005434:Flow Cytometry; D007156:Immunologic Memory / Q000187:drug effects*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D010052:Ovariectomy; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D052244:Endocrine Disruptors; D003208:Concanavalin A; D004958:Estradiol