Novel FKS mutations associated with echinocandin resistance in Candida species.
We studied three clinical isolates of Candida spp. (one C. tropicalis isolate and two C. glabrata isolates) from patients with invasive candidiasis. The first isolate emerged during echinocandin treatment, while the others emerged after the same treatment. These strains harbored an amino acid substitution in Fksp never linked before with reduced echinocandin susceptibility in C. tropicalis or in C. glabrata. The molecular mechanism of reduced susceptibility was confirmed using a 1,3-beta-D-glucan synthase inhibition assay.
Garcia-Effron
Guillermo
G
Public Health Research Institute, Newark, New Jersey, USA.
Chua
Daniel Joseph
DJ
Tomada
Jon Rupert
JR
DiPersio
Joseph
J
Perlin
David S
DS
Ghannoum
Mahmoud
M
Bonilla
Hector
H
D000328:Adult; D000935:Antifungal Agents / Q000627:therapeutic use*; D041221:Candida glabrata / Q000235:genetics*; D041022:Candida tropicalis / Q000235:genetics*; D002177:Candidiasis / Q000188:drug therapy* / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D054714:Echinocandins / Q000627:therapeutic use*; D005260:Female; D005964:Glucosyltransferases / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation
D000935:Antifungal Agents; D054714:Echinocandins; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase