Highly luminescent quantum dots functionalized and their conjugation with IgG.
A sol-gel approach including partially removing capping agents, depositing SiO(2) monomers, and growing a SiO(2) shell was developed to generate a bio-compatible functionalization on CdTe quantum dots (QDs). The QDs retained their high photoluminescence (PL) efficiency after coating with a SiO(2) shell (22.5%) by controlling the surface state of the QDs to decrease PL degradation during a sol-gel preparation. Furthermore, the CdTe QDs with a SiO(2) shell were conjugated with IgG using 3-sulfo-N-hydroxysuccinimide or streptavidin-maleimide as linkers. The biotin-streptavidin linker resulted in a high PL efficiency retained (only ~23% lower than the initial value of the QDs), which is crucial for bio-applications.
Yang
Ping
P
School of Materials Science and Engineering, University of Jinan, 250022 Jinan, PR China. mse_yangp@ujn.edu.cn
Zhang
Aiyu
A
Sun
Hongsheng
H
Liu
Futian
F
Jiang
Qinghui
Q
Cheng
Xin
X
D000818:Animals; D001710:Biotin / Q000737:chemistry; D006041:Goats; D007074:Immunoglobulin G / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry; D045663:Quantum Dots; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry
D007074:Immunoglobulin G; D008301:Maleimides; D013388:Succinimides; C043592:maleimide; D001710:Biotin; D012822:Silicon Dioxide; D019809:Streptavidin