Enhanced production of longer side-chain polyhydroxyalkanoic acid with omega-aromatic group substitution in phaZ-disrupted Pseudomonas fluorescens BM07 mutant through unrelated carbon source cometabolism and salicylic acid beta-oxidation inhibition.
The deletion of the intracellular polyhydroxyalkanoate (PHA) depolymerase gene (phaZ) in Pseudomonas fluorescens BM07 was found to increase more efficiently the levels of longer medium-chain-length (MCL) omega-aromatic monomer-units than in the wild-type strain when the cells were grown with a mixture of fructose and MCL omega-aromatic fatty acid in the presence of salicylic acid that is known as a beta-oxidation inhibitor in BM07 strain. When 11-phenoxyundecanoic acid was used as co-carbon source, the longest monomer-unit 3-hydroxy-11-phenoxyundecanoate, not reported in literature yet, was incorporated into the polymer chain up to approximately 10 mol%. An advantage of salicylic acid inhibition technique is that salicylic acid is not metabolized in BM07 strain, thus, the effective concentration of the inhibitor remaining constant throughout the cultivation. In conclusion, this new technique could be exploited for the enhanced production of side-chain modulated functional MCL-PHA with improved physicochemical properties in P. fluorescens BM07.
Nano-Biomaterials Science Laboratory, Division of Applied Life Sciences (BK21), Graduate School and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Mun Hwan
MH
Choi
Ju
J
Xu
Jong Kook
JK
Rho
Xu Ping
XP
Zhao
Sung Chul
SC
Yoon
D002210:Caprylates / Q000378:metabolism; D002244:Carbon / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation / Q000235:genetics*; D010084:Oxidation-Reduction / Q000187:drug effects; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D002210:Caprylates; D005227:Fatty Acids; D006841:Hydrocarbons, Aromatic; D054813:Polyhydroxyalkanoates; C016173:undecanoic acid; D005632:Fructose; D002244:Carbon; D020156:Salicylic Acid