Effect of UVA or UVB irradiation on cutaneous lipids in films or in solution.
The barrier function of the skin is largely due to the stratum corneum which is essentially composed of lipids. Different external factors, such as UV irradiation, affect this skin layer and are responsible for a destabilization of the supramolecular organization of its constituted lipids. In this work, mass spectrometry and infrared spectroscopy are combined to study the correlation between the formation of oxidative compounds by UV irradiation and the lipid organization. Experiments were carried out on unsaturated lipids in film or solution form, exposed to UVA or UVB irradiation. UV exposure leads to the formation of oxygenated entities in the case of lipids with an unsaturated fatty acid moiety, resulting in a decrease in their packing which is greater when the lipids are in solution. The packing decrease is even greater following UVB irradiation.
Group Chimie Analytique de Paris-Sud EA 4041, IFR 141 School of Pharmacy, Univ. Paris-Sud F, Châtenay-Malabry, France. chloe.merle@club-internet.fr
Chloé
C
Merle
Cécile
C
Laugel
Arlette
A
Baillet-Guffroy
D005231:Fatty Acids, Unsaturated / Q000528:radiation effects*; D008055:Lipids / Q000528:radiation effects*; D013058:Mass Spectrometry; D008954:Models, Biological; D010084:Oxidation-Reduction; D012867:Skin / Q000737:chemistry*; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D014466:Ultraviolet Rays / Q000009:adverse effects*
D005231:Fatty Acids, Unsaturated; D008055:Lipids; D012996:Solutions