[Construction and expression of the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217].
OBJECTIVE
To construct the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217 and express the vector in eukaryotic cells.


METHODS
ZNF217 gene fragment was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), and after analysis of the product by electrophoresis and sequencing, the fragment was inserted into pEGFP-N1 fluorescent expression vector. The constructed expression vector was then transfected into eukaryotic cells for its expression.


CONCLUSIONS
Restriction endonuclease digestion and sequence analysis confirmed correct construction of the recombinant vector pEGFP-N1 and the expression vector pEGFP-N1-ZNF217, which can be stably expressed in eukaryotic cells.
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lijing7405@126.com
Jing
J
Li
Jun
J
Zhou
Mei
M
Zhong
D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000473:pathology; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C113698:ZNF217 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins