Isl-1 down-regulates DRG cell proliferation during chicken embryo development.
OBJECTIVE
Protein Isl-1 RNA interference and over expression in early chicken embryo dorsal root ganglia (DRG) were used to investigate the function of Isl-1 in DRG cell proliferation.


METHODS
Isl-1 targeted shRNA expression vector and Isl-1 over-expression vector were transfected into chicken embryo DRG by in ovo electroporation. Then, the DRG proliferation rate was detected by BrdU immunohistochemistry.


RESULTS
The rate of DRG cell proliferation increased after Isl-1 knock-down and decreased after Isl-1 over-expression.


CONCLUSIONS
In this study, we found that Isl-1 negatively modulates DRG cell proliferation.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Dawei
D
Chen
Guoxin
G
Wang
Haoshu
H
Luo
Jiali
J
Liu
Sheng
S
Cui
D000818:Animals; D030801:Animals, Genetically Modified; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo / Q000378:metabolism / Q000502:physiology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D005727:Ganglia, Spinal / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D060850:LIM-Homeodomain Proteins; D034741:RNA, Small Interfering / Q000494:pharmacology; D014157:Transcription Factors
D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1