Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin.
Cylindrospermopsin (CYN) is a cyanotoxin which has been implicated in human intoxication and animal mortality. Genotoxic activity of this hepatotoxin is known but its carcinogenic activity remains to be elucidated. In this work, CYN was assessed for its cell-transforming activity using the Syrian hamster embryo (SHE) cell transformation assay. This in vitro assay is used to evaluate the carcinogenic potential of chemical, physical and biological agents in SHE cells, which are primary, normal, diploid, genetically stable and capable of metabolic activation. We demonstrated that CYN induced a significant increase in morphological cell transformation in SHE cells following a 7-day continuous treatment in the range of non-cytotoxic concentrations 1 x 10(-7)-1 x 10(-2) ng/mL.
Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes (LIEBE), Université Paul Verlaine de METZ, UMR CNRS 7146, Rue du Général Delestraint, 57070 Metz, France. marie-aline.maire@univ-metz.fr
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D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins; D001711:Biotransformation / Q000187:drug effects; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002477:Cells / Q000187:drug effects / Q000648:ultrastructure; D002999:Clone Cells; D006224:Cricetinae; D000087522:Cyanobacteria Toxins; D008647:Mesocricetus; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D014498:Uracil / Q000031:analogs & derivatives* / Q000633:toxicity
D000470:Alkaloids; D001427:Bacterial Toxins; D002273:Carcinogens; D000087522:Cyanobacteria Toxins; D009153:Mutagens; C089595:cylindrospermopsin; D014498:Uracil