[Detection of periodental pathogens in atherosclerotic lesions in patients].
OBJECTIVE
To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction (PCR) techniques.


METHODS
Ten human specimens of atherosclerotic plaque were obtained sterilely. The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples. To detect Porphyromonas gingivalis(Pg), Tannerella forsythia(Tf), Aggregatibacter actinomycetemcomitans(Aa), Prevotella intermedia(Pi), Prevotella nigrescens(Pn), Treponema denticola(Td), Campylobacter rectus(Cr), PCR amplification of bacterial 16S ribosomal DNA (rDNA) was carried out.


RESULTS
PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens. DNA of Pg were found in 3 samples, Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples.


CONCLUSIONS
The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques. Pg, Tf, Pn may play a role in the development and progression of atherosclerosis in these Chinese patients. Further studies with large size samples are needed.
An
Na
N
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Ou-yang
Xiang-ying
XY
Han
Wei
W
D000368:Aged; D000369:Aged, 80 and over; D050197:Atherosclerosis / Q000382:microbiology* / Q000473:pathology; D055113:Chronic Periodontitis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D018713:Prevotella intermedia / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S