[Evaluation of Powerplex Y system in genotyping of male component after sexual intercourse].
Y-STRs are of an importance in forensic genetics in cases of selective genotyping of male haplotypes and/or determination of a number of perpetrators. Experimental vaginal swabs containing semen were collected to evaluate the effect of storage conditions and male/female DNA ratio on the efficiency of amplification of Powerplex Y system alleles. The specimens were collected on disposable devices from 20 volunteer pairs of sexual partners. The swabs were stored on selected supports in different temperature conditions. DNA templates were extracted using Chelex and quantitated spectrophotometrically. Powerplex Y system (Promega) ABI Prism 310 (Applera) were used to obtain Y-STR profiles. Vaginal swabs airdryed at room temperature were found the best source of DNA for male identification. Full Y-STR haplotypes were obtained in male/female mixtures prepared in proportion of 1 : 1000.
Ptaszyńska-Sarosiek
Iwona
I
Zakład Medycyny Sadowej Akademii Medycznej w Białymstoku, ul. Kilińskiego 1, 15-230 Białystok.
Niemcunowicz-Janica
Anna
A
Pepiński
Witold
W
Skawrońska
Małgorzata
M
Koc-Zórawska
Ewa
E
Janica
Jerzy
J
D041322:Chromosomes, Human, Y / Q000235:genetics*; D003075:Coitus / Q000502:physiology*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D011902:Rape / Q000175:diagnosis; D012661:Semen / Q000737:chemistry*; D013048:Specimen Handling; D020080:Tandem Repeat Sequences; D014626:Vaginal Smears / Q000379:methods*