Coordinated transcriptional regulation of the divinyl ether biosynthetic genes in tobacco by signal molecules related to defense.
In tobacco, 9-divinyl ethers (DVEs) produced by the lipoxygenase NtLOX1 and DVE synthase NtDES1 are important for full resistance to pathogens. In this work, the regulation of NtLOX1 and NtDES1 expression by signal molecules was investigated in LOX1 promoter-reporter transgenic plants and by RT-qPCR. Methyl jasmonate, ACC and elicitor were shown to coordinately trigger the DVE pathway. Induction was strongly attenuated in the presence of salicylic acid, which seems to act as a negative regulator of the 9-DVE biosynthetic enzymes. Our data suggest that, in tobacco, DVE biosynthesis is cross-regulated by jasmonates, and by other hormonal and signal molecules such as ethylene and SA.
University of Turin, DiVaPRA - Plant Pathology, via L. da Vinci 44, I-10095 Grugliasco (TO), Italy.
Alessandro
A
Fammartino
Bertrand
B
Verdaguer
Joëlle
J
Fournier
Giacomo
G
Tamietti
Francis
F
Carbonne
Marie-Thérèse
MT
Esquerré-Tugayé
Francesca
F
Cardinale
D000085:Acetates / Q000378:metabolism; D000598:Amino Acids, Cyclic / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics*; D008084:Lipoxygenase / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014753:Vinyl Compounds / Q000378:metabolism*
D000085:Acetates; D000598:Amino Acids, Cyclic; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014753:Vinyl Compounds; C100195:vinyl ether; C023863:1-aminocyclopropane-1-carboxylic acid; C072239:methyl jasmonate; D003577:Cytochrome P-450 Enzyme System; C100485:divinyl ether synthase; D008084:Lipoxygenase; D020156:Salicylic Acid