Effects of flavin7 on allergen induced hyperreactivity of airways.
Some studies have suggested that the polyphenolic compounds might reduce the occurrence of asthma symptoms. The aim of our experiments was to evaluate the effects of 21 days of the flavonoid Flavin7 administration on experimentally induced airway inflammation in ovalbumin-sensitized guinea pigs. We assessed tracheal smooth muscle reactivity by an in vitro muscle-strip method; changes in airway resistance by an in vivo plethysmographic method; histological picture of tracheal tissue; and the levels of interleukin 4 (IL-4), and interleukin 5 (IL-5) in bronchoalveolar lavage fluid (BALF). Histological investigation of tracheal tissue and the concentrations of the inflammatory cytokines IL-4 and IL-5 in BALF were used as indices of airway inflammation. Administration of Flavin7 caused a significant decrease of specific airway resistance after histamine nebulization and a decline in tracheal smooth muscle contraction amplitude in response to bronchoconstricting mediators. Flavin7 minimized the degree of inflammation estimated on the basis of eosinophil calculation and IL-4 and IL-5 concentrations. In conclusion, administration of Flavin7 showed bronchodilating and anti-inflammatory effects on allergen-induced airway inflammation.
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia. Franova@jfmed.uniba.sk
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Franova
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Joskova
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Novakova
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Adamicova
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Sutovska
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Nosal
D000818:Animals; D016535:Bronchial Hyperreactivity / Q000188:drug therapy* / Q000276:immunology; D016207:Cytokines / Q000502:physiology; D005419:Flavonoids / Q000627:therapeutic use*; D006168:Guinea Pigs; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D013267:Stilbenes / Q000627:therapeutic use*
D016207:Cytokines; C531446:Flavin7; D005419:Flavonoids; D013267:Stilbenes; D010047:Ovalbumin