Cytotoxic and anti-inflammatory cembranoids from the Dongsha Atoll soft coral Sarcophyton crassocaule.
Five new cembranoids, sarcocrassocolides A-E (1-5), along with three known cembranoids 6-8, have been isolated from a Formosan soft coral Sarcophyton crassocaule. The structures of the new metabolites were elucidated by extensive spectroscopic analysis and the absolute configuration of 1 was determined by a modified Mosher's method. Compounds 1-4 exhibited significantly cytotoxic activity against a limited panel of cancer cell lines. Compounds 1-4, 6 and 8 were shown to exert significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells. Compound 6 also significantly inhibited the accumulation of pro-inflammatory COX-2 protein.
Lin
Wan-Yu
WY
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Su
Jui-Hsin
JH
Lu
Yi
Y
Wen
Zhi-Hong
ZH
Dai
Chang-Feng
CF
Kuo
Yao-Haur
YH
Sheu
Jyh-Horng
JH
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008968:Molecular Conformation
D000893:Anti-Inflammatory Agents; D004224:Diterpenes; C037071:cembrane; D051546:Cyclooxygenase 2