Microtubule-dependent localization of profilin I mRNA to actin polymerization sites in serum-stimulated cells.
Specific localization of messenger RNA (mRNA) appears to be a general mechanism to accumulate certain proteins to subcellular compartments for participation in local processes, thereby maintaining cell polarity under strict spatiotemporal control. Transportation of mRNA with associated protein components (RNP granules) by the actin microfilament or the microtubule systems is one important mechanism to achieve this locally distributed protein production. Here we provide evidence for a microtubule-dependent localization of mRNA encoding the actin regulatory protein profilin to sites in mouse embryonic fibroblasts, which express enhanced actin polymerization.
Johnsson
Anna-Karin
AK
Department of Cell Biology, WGI, Stockholm University, SE-106 91 Stockholm, Sweden.
Karlsson
Roger
R
D000199:Actins / Q000378:metabolism*; D000818:Animals; D022081:Cell Surface Extensions / Q000187:drug effects / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D051304:Profilins / Q000235:genetics / Q000378:metabolism*; D034443:RNA Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044967:Serum / Q000378:metabolism*
D000199:Actins; D051304:Profilins; D012333:RNA, Messenger; D003571:Cytochalasin B; D015739:Nocodazole