Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test.
We studied the utility of performing a penicillin binding protein 2a latex agglutination (PBP-LA) assay directly on Bactec blood culture broth samples containing Staphylococcus aureus to rapidly detect methicillin resistance. The sensitivity, specificity, positive predictive value, and negative predictive value of this method were 94.1%, 97.5%, 98%, and 92.9%, respectively.
Qian
Qinfang
Q
Division of Laboratory and Transfusion Medicine, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA. qqian@bidmc.harvard.edu
Venkataraman
Lata
L
Kirby
James E
JE
Gold
Howard S
HS
Yamazumi
Toshiaki
T
D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry* / Q000302:isolation & purification*; D046915:Penicillin-Binding Proteins / Q000032:analysis* / Q000276:immunology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins