Failure of xenoimplantation using porcine synovium-derived stem cell-based cartilage tissue constructs for the repair of rabbit osteochondral defects.
The use of xenogeneic tissues offers many advantages with respect to availability, quality control, and timing of tissue harvest. Our previous study indicated that implantation of premature tissue constructs from allogeneic synovium-derived stem cells (SDSCs) facilitated cartilage tissue regeneration. The present study investigated the feasibility of xenoimplantation of SDSC-based premature tissue constructs for the repair of osteochondral defects. Porcine SDSCs were mixed with fibrin gel, seeded in polyglycolic acid (PGA) scaffolds, and cultured in a rotating bioreactor system supplemented for 1 month with growth factor cocktails. The engineered porcine premature tissues were implanted to repair surgically induced osteochondral defects in the medial femoral condyles of 12 rabbits. Three weeks after surgery, the xenoimplantation group exhibited a smooth, whitish surface while the untreated control remained empty. Surprisingly, 6 months after surgery, the xenoimplantation group displayed some tissue loss while the untreated control group was overgrown with fibrocartilage tissue. In the xenoimplantation group, chronic inflammation was observed in synovial tissue where porcine major histocompatibility complex (MHC) class II antigen positively stained in the engulfed foreign bodies. In addition, porcine source cells also migrated from the implantation site and may have been responsible for the observed loss of glycosaminoglycans (GAGs) underneath surrounding articular cartilage. The histological score was much worse in the xenoimplanted group than in the untreated control. Our study suggested that SDSC-based xenogeneic tissue constructs might cause delayed immune rejection. Xenotransplantation may not be an appropriate approach to repair osteochondral defects.
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, P.O. Box 9196, One Medical Center Drive, Morgantown, West Virginia 26506-9196, USA. mpei@hsc.wvu.edu
Ming
M
Pei
Zuoqin
Z
Yan
Mark
M
Shoukry
Brandon M
BM
Boyce
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D007718:Knee Injuries / Q000601:surgery*; D008297:Male; D010007:Osteochondritis / Q000628:therapy; D011817:Rabbits; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D013552:Swine; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous / Q000009:adverse effects*