An instrument-based screening assay for DNA-targeted anticancer drugs using resonance light scattering.
DNA is a valid drug target for development of target-based therapeutics against cancer. Screening DNA-targeted anticancer drugs is a key process for the research and development of new anticancer drugs. The traditional anticancer drug screening methods, including animal experiments and cell-based screening assays, have unavoidable drawbacks. In this contribution, the new instrument-based screening assay for DNA-targeted anticancer drugs in vitro using resonance light scattering (RLS) technique was proposed. The experiments suggested that the increment of RLS intensity was directly proportional to the antitumor effect of anticancer drugs. Therefore, it was intuitive to obtain the sequence of the antitumor activity of four anticancer drugs without data processing as follows: mitoxantrone (MIT) > pirarubicin (PIR) > daunorubicin (DAU) > doxorubicin (DOX) by RLS screening spectra. Moreover, the apparent equilibrium constant (K) was 1.23 x 10(4), 2.22 x 10(4), 4.66 x 10(4) L/mol for DOX, DAU, and PIR, respectively. The inhibitory concentration 50 (IC50) was 0.148, 0.102, 0.025, 0.013 micromol/L for DOX, DAU, PIR, MIT, respectively. Therefore, the antitumor effect of four drugs was as follows: MIT > PIR > DAU > DOX, which was in good agreement with the result obtained from RLS screening assays. The mechanism between DNA and anthracycline drugs was investigated using UV-vis spectroscopy, fluorescence spectroscopy, and electrophoresis experiments. The proposed assay is a rapid, intuitive, and easy-to-conduct bioassay with good accuracy and reproducibility.
Department of Chemistry, Shantou University, Shantou, China. kqlu@stu.edu.cn
Zhanguang
Z
Chen
Guoliang
G
Liu
Maohuai
M
Chen
Xi
X
Chen
Mingyao
M
Wu
Xingtong
X
Chen
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis* / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation* / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D009994:Osmolar Concentration; D010957:Plasmids; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation
D000970:Antineoplastic Agents; D004247:DNA