The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans.
Carbon dioxide (CO(2)) and its hydration product bicarbonate (HCO(3)(-)) are essential molecules in various physiological processes of all living organisms. The reversible interconversion between CO(2) and HCO(3)(-) is in equilibrium. This reaction is slow without catalyst, but can be rapidly facilitated by Zn(2+)-metalloenzymes named carbonic anhydrases (CAs). To gain an insight into the function of multiple clades of fungal CA, we chose to investigate the filamentous fungi Aspergillus fumigatus and A. nidulans. We identified four and two CAs in A. fumigatus and A. nidulans, respectively, named cafA-D and canA-B. The cafA and cafB genes are constitutively, strongly expressed whereas cafC and cafD genes are weakly expressed but CO(2)-inducible. Heterologous expression of the A. fumigatus cafB, and A. nidulans canA and canB genes completely rescued the high CO(2)-requiring phenotype of a Saccharomyces cerevisiae Deltance103 mutant. Only the DeltacafA DeltacafB and DeltacanB deletion mutants were unable to grow at 0.033% CO(2), of which growth defects can be restored by high CO(2). Defects in the CAs can affect Aspergilli conidiation. Furthermore, A. fumigatus DeltacafA, DeltacafB, DeltacafC, DeltacafD and DeltacafA DeltacafB mutant strains are fully virulent in a low-dose murine infection.
Han
Kap-Hoon
KH
Department of Pharmaceutical Engineering, Woosuk University, Wanju, Korea.
Chun
Yoon-Hee
YH
Figueiredo
Bárbara de Castro Pimentel
Bde C
Soriani
Frederico Marianetti
FM
Savoldi
Marcela
M
Almeida
Agostinho
A
Rodrigues
Fernando
F
Cairns
Charlie Timothy
CT
Bignell
Elaine
E
Tobal
Jaqueline Moisés
JM
Goldman
Maria Helena S
MH
Kim
Jong-Hwan
JH
Bahn
Yong-Sun
YS
Goldman
Gustavo Henrique
GH
Ferreira
Márcia Eliana da Silva
ME
D000595:Amino Acid Sequence; D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000473:pathology; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000235:genetics; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D013172:Spores, Fungal / Q000254:growth & development; D016019:Survival Analysis; D014774:Virulence
D004271:DNA, Fungal; D005656:Fungal Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases