[The sonic hedgehog induce vascular adventitial fibroblasts phenotypic modulation, proliferation and migration].
OBJECTIVE
To study the effect of shh on the migration, proliferation and phenotypic modulation of vascular adventitial fibroblasts.


METHODS
Cultivate the vascular adventitial fibroblast in vitro. Use immunofluorescent, laser confocal microscopy, Western-blot and real-time PCR to detect the expression of mRNA and protein of related index. Estimate cell proliferation according to the expression of Ki67 and cell proliferation curve. Application of wound healing test to estimate migration of fibroblast. The expression of alpha-actin is thought to be marker of phenotypic modulation of fibroblast.


RESULTS
The expression of shh was detected in vascular adventitial fibroblast in vitro. After addition of exogenous shh (3.5 microg/ml), there were more Ki67(+) cells and the wounding area which was covered by cells became larger. The expression of alpha-actin was detected. After addition of cyclopamine (40 micromol/L), there were less Ki67(+) cells and the wounding area which was covered by cells became smaller.


CONCLUSIONS
Shh can promote proliferation, migration and phenotypic modulation of vascular adventitial fibroblasts.
Li
Feng-he
FH
Department of Vascular Surgery, First Affiliated Hospital, China Medical University, Shenyang, China.
Xin
Shi-jie
SJ
Zhang
Si-yang
SY
Cui
Ze-shi
ZS
Gao
Jian
J
Zhang
Jian
J
Duan
Zhi-quan
ZQ
D001808:Blood Vessels; D002454:Cell Differentiation; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D053823:Hedgehog Proteins / Q000494:pharmacology*; D006801:Humans
D053823:Hedgehog Proteins