Confocal immunocytochemistry of embryonic and juvenile Hawaiian bobtail squid (Euprymna scolopes) tissues.
The Hawaiian bobtail squid Euprymna scolopes is a cephalopod whose small size, short lifespan, rapid growth, and year-round availability make it suitable as a model organism. This protocol describes the preparation of whole juvenile squids by whole-mount immunocytochemistry for visualization by confocal microscopy.
Lee
Patricia N
PN
Department of Zoology, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
McFall-Ngai
Margaret J
MJ
Callaerts
Patrick
P
de Couet
H Gert
HG
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D049832:Decapodiformes / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D006254:Hawaii; D007150:Immunohistochemistry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D009928:Organ Specificity; D016707:Tissue Fixation
D000906:Antibodies