Induction of tumoricidal activity in mouse peritoneal macrophages by ginseng polysaccharide.
This study examined the effects of ginseng polysaccharide (GPS) on mouse peritoneal macrophage (PM)-mediated cytotoxicity towards K562, HL-60, or KG1alpha cells. GPS had no direct effect on killing of tumor cells. However, when mouse PMs were treated with GPS, cytotoxic activity against K562, HL-60, or KG1alpha cells was significantly induced. In addition, phagocytic activity was enhanced in GPS-treated mouse PMs compared to the control. The expressions of CD(68), ACP and alpha-ANE in mouse PMs were increased by the treatment with GPS. Moreover, the levels of cytokines, including tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), IL-6 were increased and the production of nitric oxide (NO) was enhanced. Taken together, these results suggest that GPS possess a potent antitumor activity by stimulating macrophage and a potentiality as an immunomodulator against diseases such as cancer.
Department of Histology and Embryology, Chongqing Medical University, Yuzhong District, China.
J
J
Wang
G
G
Zuo
J
J
Li
T
T
Guan
C
C
Li
R
R
Jiang
B
B
Xie
X
X
Lin
F
F
Li
Y
Y
Wang
D
D
Chen
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects* / Q000276:immunology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis; D005894:Panax / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*
D000970:Antineoplastic Agents; D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide