Nano aptasensor for protective antigen toxin of anthrax.
We demonstrate a highly sensitive nano aptasensor for anthrax toxin through the detection of its polypeptide entity, protective antigen (PA toxin) using a PA toxin ssDNA aptamer functionalized single-walled carbon nanotubes (SWNTs) device. The aptamer was developed in-house by capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) and had a dissociation constant (K(d)) of 112 nM. The aptasensor displayed a wide dynamic range spanning up to 800 nM with a detection limit of 1 nM. The sensitivity was 0.11 per nM, and it was reusable six times. The aptasensor was also highly selective for PA toxin with no interference from human and bovine serum albumin, demonstrating it as a potential tool for rapid and point-of-care diagnosis for anthrax.
Cella
Lakshmi N
LN
Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
Sanchez
Pablo
P
Zhong
Wenwan
W
Myung
Nosang V
NV
Chen
Wilfred
W
Mulchandani
Ashok
A
D000881:Anthrax / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D015374:Biosensing Techniques; D019075:Electrophoresis, Capillary; D036103:Nanotechnology
D000942:Antigens, Bacterial