2-D RP/RPLC method to separate components in Fructus schisandrae chinensis.
An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click beta-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. Beta-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click beta-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click beta-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Gaowa
G
Jin
Yuntao
Y
Dai
Jiatao
J
Feng
Xuemei
X
Qin
Xingya
X
Xue
Feifang
F
Zhang
Xinmiao
X
Liang
D002853:Chromatography, Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D032141:Schisandra / Q000737:chemistry*