Synthesis of poly(vinyl alcohol)/C(60) and poly(N-vinylpyrrolidone)/C(60) nanohybrids as potential photodynamic cancer therapy agents.
Well-defined poly(vinyl acetate) (PVAc) and poly(N-vinylpyrrolidone)-co-poly(vinyl acetate) (PNVP-co-PVAc) chains end-capped by Co(acac)(2) (acac=acetylacetonate) and prepared by cobalt-mediated radical polymerization (CMRP) are grafted onto a fullerene. Homolytic Co-C bond cleavage of the polymer chain ends at 30 degrees C releases the polymeric radicals that add onto C(60), thereby leading to the corresponding PVAc/C(60) and PNVP-co-PVAc/C(60) nanohybrids. The [polymer-Co(acac)(2)]/[C(60)] molar ratio was varied to adjust the structure of the nanohybrids, and more particularly the number of grafted arms. Finally, the potential of the hydrosoluble PVOH/C(60) nanohybrids, which result from the methanolysis of the ester groups of PVAc/C(60), and of the PNVP-co-PVAc/C(60) nanohybrids as photosensitizers for photodynamic therapy (PDT), was approached. First, photobleaching tests demonstrated the ability of these nanohybrids to produce singlet oxygen upon irradiation, which can play a role in cell damage. Second, cell viability assays demonstrated that both types of nanohybrids are deprived of intrinsic cytotoxicity in the dark, whereas they promoted significant cell mortality when subjected to light treatment. The selective response of these materials to irradiation makes them promising compounds for PDT.
Center for Education and Research on Macromolecules (CERM), University of Liège, Sart-Tilman, B6a, 4000 Liège, Belgium.
Marie
M
Hurtgen
Antoine
A
Debuigne
Ange
A
Mouithys-Mickalad
Robert
R
Jérôme
Christine
C
Jérôme
Christophe
C
Detrembleur
D002460:Cell Line; D003035:Cobalt / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011760:Pyrrolidinones / Q000737:chemistry*; D011789:Quantum Theory; D026082:Singlet Oxygen / Q000378:metabolism
D017319:Photosensitizing Agents; D011760:Pyrrolidinones; D026082:Singlet Oxygen; D003035:Cobalt; C042670:N-vinyl-2-pyrrolidinone; D011142:Polyvinyl Alcohol