Trans fatty acid-induced NF-kappaB activation does not induce insulin resistance in cultured murine skeletal muscle cells.
Long-chain saturated fatty acids such as palmitic acid induce insulin resistance and NF-kappaB activation in skeletal muscle cells. Here we investigated the effects of long-chain fatty acid (FA) saturation and configuration on NF-kappaB activity and insulin sensitivity in cultured skeletal muscle cells. Of all tested unsaturated FAs, only elaidic acid (3-fold), cis9,trans11-CLA (3-fold) and trans10,cis12-CLA (13-fold) increased NF-kappaB transactivation in myotubes. This was not accompanied by decreased insulin sensitivity (measured as insulin-induced glucose uptake and GLUT4 translocation). We therefore conclude that FA-induced NF-kappaB activation is not sufficient for the induction of insulin resistance in skeletal muscle cells.
Hommelberg
Pascal P H
PP
Department of Human Biology, Nutrim School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. pascal_hommelberg@yahoo.com
Langen
Ramon C J
RC
Schols
Annemie M W J
AM
van Essen
Anon L M
AL
Snepvangers
Frank J M
FJ
Mensink
Ronald P
RP
Plat
Jogchum
J
D000818:Animals; D002478:Cells, Cultured; D007333:Insulin Resistance / Q000502:physiology*; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D044242:Trans Fatty Acids / Q000737:chemistry / Q000378:metabolism*
D016328:NF-kappa B; D044242:Trans Fatty Acids