Part 1. Spectrophotometric determination of trace mercury (II) in dental-unit wastewater and fertilizer samples using the novel reagent 6-hydroxy-3-(2-oxoindolin-3-ylideneamino)-2-thioxo-2H-1,3-thiazin-4(3H)-one and the dual-wavelength beta-correction spectrophotometry.
A simple and low cost method was developed and validated for the determination of trace mercury (II) ions in dental-unit wastewater and fertilizer samples. The method was based upon the reaction of mercury (II) ions with the novel reagent 6-hydroxy-3-(2-oxoindolin-3-ylideneamino)-2-thioxo-2H-1,3-thiazin-4(3H)-one, the formed complex shows an absorption maximum at 505 nm (lambda(max)) in Britton-Robinson (B-R) buffer (pH 4-6).The corrected absorbance of the formed complex at lambda(max) was obtained employing beta-correction spectrophotometric method. Beer's-Lambert law and Ringbom's plots of the colored Hg-reagent complex were obeyed in the concentration range of 0.2-2.0 and 0.32-0.96 microg mL(-1) mercury (II) ions, respectively with a relative standard deviation in the range of 2.1+/-1.3%. The limits of detection (LOD) and quantification (LOQ) of the procedure were 0.026 and 0.086 microg mL(-1) Hg(2+), respectively. The proposed method was applied for the analysis of mercury (II) in dental-unit wastewater and fertilizer samples. The validation of the method was tested by comparison with the data obtained by the inductively coupled plasma-mass spectrometry (ICP-MS). The statistical treatment of data in terms of Student's t-tests and variance ratio f-tests has revealed no significance differences.
Hamza
A
A
Department of Chemistry, Faculty of Science, King Abdulaziz University, P. O. Box 80203, Jeddah 21589, Saudi Arabia.
Bashammakh
A S
AS
Al-Sibaai
A A
AA
Al-Saidi
H M
HM
El-Shahawi
M S
MS
D000042:Absorption; D002614:Chelating Agents / Q000737:chemistry*; D003813:Dentistry; D005308:Fertilizers / Q000032:analysis*; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008506:Medical Waste / Q000032:analysis*; D008628:Mercury / Q000032:analysis*; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013843:Thiazines / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
C550715:6-hydroxy-3-(2-oxoindolin-3-ylideneamino)-2-thioxo-2H-1,3-thiazin-4(3H)-one; D002614:Chelating Agents; D005308:Fertilizers; D007202:Indicators and Reagents; D007211:Indoles; D008506:Medical Waste; D013843:Thiazines; D014874:Water Pollutants, Chemical; D008628:Mercury