Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli.
This is the first report of glycoside hydrolase family 43 beta-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH.
Suzuki
Satoshi
S
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.
Fukuoka
Mari
M
Ookuchi
Hikaru
H
Sano
Motoaki
M
Ozeki
Kenji
K
Nagayoshi
Emi
E
Takii
Yukio
Y
Matsushita
Mayumi
M
Tada
Sawaki
S
Kusumoto
Ken-Ichi
K
Kashiwagi
Yutaka
Y
D001236:Aspergillus oryzae / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D013379:Substrate Specificity; D014995:Xylosidases / Q000378:metabolism*
D005656:Fungal Proteins; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase