CEP215 is involved in the dynein-dependent accumulation of pericentriolar matrix proteins for spindle pole formation.
CEP 215 is a human orthologue of Drosophila centrosomin which is a core centrosome component for the pericentriolar matrix protein recruitment. Recent investigations revealed that CEP 215 is required for centrosome cohesion, centrosomal attachment of the gamma-TuRC, and microtubule dynamics. However, it remains obscure how CEP 215 functions for recruitment of the centrosomal proteins during the centrosome cycle. Here, we investigated a role of CEP 215 during mitosis. Knockdown of CEP 215 resulted in characteristic mitotic phenotypes, including monopolar spindle formation, a decrease in distance between the spindle pole pair, and detachment of the centrosomes from the spindle poles. We noticed that CEP 215 is critical for centrosomal localization of dynein throughout the cell cycle. As a consequence, the selective centrosomal proteins were not recruited to the centrosome properly. Finally, the centrosomal localization of CEP 215 also depends on the dynein-dynactin complex. Based on the results, we propose that CEP 215 regulates a dynein-dependent transport of the pericentriolar matrix proteins during the centrosome maturation.
Department of Biological Sciences, Seoul National University, Seoul, Korea.
Seongju
S
Lee
Kunsoo
K
Rhee
D018797:Cell Cycle Proteins; D018385:Centrosome / Q000378:metabolism; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
C405929:CDK5RAP2 protein, human; D018797:Cell Cycle Proteins; D000072159:Dynactin Complex; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D004398:Dyneins