The yeast formin Bnr1p has two localization regions that show spatially and temporally distinct association with septin structures.
Formins are conserved eukaryotic proteins that direct the nucleation and elongation of unbranched actin filaments. The yeast formins, Bni1p and Bnr1p, assemble actin cables from the bud cortex and bud neck, respectively, to guide overall cell polarity. Here we examine the regions of Bnr1p responsible for bud neck localization. We define two non-overlapping regions, Bnr1p-L1 (1-466) and Bnr1p-L2 (466-733), that can each localize to the bud neck independently of endogenous Bnr1p. Bnr1p-L1 and Bnr1p-L2 localize with septins at the bud neck, but show slightly differently spatial and temporal localization, reflecting the localization (Bnr1p-L1) or cell cycle timing (Bnr1p-L2) of full-length Bnr1p. Bnr1p is known to be very stably localized at the bud neck, and both Bnr1p-L1 and Bnr1p-L2 also show relatively stable localization there. Overexpression of Bnr1p-L1, but not Bnr1p-L2, disrupts septin organization at the bud neck. Thus Bnr1p has two separable regions that each contribute to its bud neck localization.
Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA.
Lina
L
Gao
Wenyu
W
Liu
Anthony
A
Bretscher
D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D020798:Two-Hybrid System Techniques
D000199:Actins; C106463:BNR1 protein, S cerevisiae; D003598:Cytoskeletal Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins