Bioconversion of the antihistaminc drug loratadine by tobacco cell suspension cultures expressing human cytochrome P450 3A4.
In this study we have expanded the metabolic potential of plant cell suspension cultures by introducing active human cytochrome P450 monooxygenase 3A4 into tobacco cells. Exogenously supplied loratadine was metabolized in a 3A4-specific manner, showing the capacity of this system for the generation of metabolites.
Warzecha
Heribert
H
Technische Universitaet Darmstadt, Department for Biology, Schnittspahnstr.3-5, 65287 Darmstadt, Germany. warzecha@bio.tu-darmstadt.de
Ferme
Daniela
D
Peer
Markus
M
Frank
Andreas
A
Unger
Matthias
M
D051544:Cytochrome P-450 CYP3A / Q000235:genetics* / Q000378:metabolism*; D006633:Histamine Antagonists / Q000378:metabolism*; D006801:Humans; D017336:Loratadine / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000502:physiology*
D006633:Histamine Antagonists; D011994:Recombinant Proteins; D017336:Loratadine; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human