Injection of lentivirus into stage-X blastoderm for the production of transgenic quail.
This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.
Division of Biology and Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Greg
G
Poynter
David
D
Huss
Rusty
R
Lansford
D000818:Animals; D030801:Animals, Genetically Modified; D001756:Blastoderm / Q000166:cytology*; D018929:Cell Culture Techniques; D003370:Coturnix / Q000235:genetics*; D018014:Gene Transfer Techniques; D005854:Germ Cells / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics*; D010063:Ovum / Q000502:physiology*