Non-homologous end-joining gene profiling reveals distinct expression patterns associated with lymphoma and multiple myeloma.
Repair of DNA strand breaks induced during lymphoid antigen receptor rearrangement involves non-homologous end-joining (NHEJ). We investigated NHEJ in the aetiology of lymphoproliferative disorders (LPDs) and the disease subtypes therein through real-time quantitative RT-PCR gene expression analysis. Lower expression of XRCC6 and MRE11A was observed in all tumours, with higher expression of both XRCC4 and RAD50 observed only in multiple myeloma (MM). Hierarchical clustering enabled tumours to be clearly distinguished from controls, and by morphological sub-type. We postulate this identifies targets worthy of investigation in the genetic predisposition, pathogenesis and prognosis of lymphoid malignancies.
Roddam
Philippa L
PL
The Children's Foundation Research Center, Le Bonheur Children's Medical Center, TN 38103, USA. proddam@uthsc.edu
Allan
James M
JM
Dring
Ann M
AM
Worrillow
Lisa J
LJ
Davies
Faith E
FE
Morgan
Gareth J
GJ
D017766:Acid Anhydride Hydrolases; D034961:Antigens, Nuclear / Q000096:biosynthesis / Q000235:genetics; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000096:biosynthesis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D020022:Genetic Predisposition to Disease; D006801:Humans; D000072200:Ku Autoantigen; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism*; D000076228:MRE11 Homologue Protein; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C121766:MRE11 protein, human; D009363:Neoplasm Proteins; C101467:XRCC4 protein, human; D000076228:MRE11 Homologue Protein; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen; D045643:DNA Repair Enzymes