Transversal stiffness and Young's modulus of single fibers from rat soleus muscle probed by atomic force microscopy.
The structural integrity of striated muscle is determined by extra-sarcomere cytoskeleton that includes structures that connect the Z-disks and M-bands of a sarcomere to sarcomeres of neighbor myofibrils or to sarcolemma. Mechanical properties of these structures are not well characterized. The surface structure and transversal stiffness of single fibers from soleus muscle of the rat were studied with atomic force microscopy in liquid. We identified surface regions that correspond to projections of the Z-disks, M-bands, and structures between them. Transversal stiffness of the fibers was measured in each of these three regions. The stiffness was higher in the Z-disk regions, minimal between the Z-disks and the M-bands, and intermediate in the M-band regions. The stiffness increased twofold when relaxed fibers were maximally activated with calcium and threefold when they were transferred to rigor (ATP-free) solution. Transversal stiffness of fibers heavily treated with Triton X-100 was about twice higher than that of the permeabilized ones, however, its regional difference and the dependence on physiological state of the fiber remained the same. The data may be useful for understanding mechanics of muscle fibers when it is subjected to both axial and transversal strain and stress.
Ogneva
Irina V
IV
State Scientific Center of Russian Federation, Institute for Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. iogneva@yandex.ru
Lebedev
Dmitry V
DV
Shenkman
Boris S
BS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000388:Air; D000465:Algorithms; D000818:Animals; D002118:Calcium / Q000378:metabolism; D055119:Elastic Modulus; D004548:Elasticity; D008297:Male; D018625:Microscopy, Atomic Force / Q000379:methods; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000502:physiology*; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D009465:Neuromuscular Agents / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma / Q000187:drug effects / Q000502:physiology
D009465:Neuromuscular Agents; D000255:Adenosine Triphosphate; D017830:Octoxynol; D002118:Calcium