Use of 99mTc-anti-CD3 scintigraphy in the differential diagnosis of rheumatic diseases.
OBJECTIVE
The aim of this study was to assess the use of anti-CD3, labelled with technetium-99m scintigraphy, for evaluating the joints of patients with RA, juvenile idiopathic arthritis (JIA), OA and gouty arthritis, and to establish the diagnosis parameters for each disease.


METHODS
We evaluated 2044 joints from 77 patients with rheumatic diseases. The clinical evaluation consisted of laboratory assays; examination for joint inflammation (pain and/or oedema); and for patients with RA, the disease activity score of 28 joints. To evaluate the sensitivity and specificity of 99mTc-anti-CD3 in detecting disease activity, patients received an injection of the radiopharmaceutical compound 99mTc-anti-CD3, and underwent a scintigraphy scan 1 h later. Scanning was repeated 3 h later. As a control, after 2 days, the patient was injected with 99mTc-non-specific human immunoglobulins, and scintigraphy scanning performed at 1 and 3 h after the injection. The intensity of uptake and the pattern of activity were defined, and Spearman's correlation and analysis of variance used for statistical evaluation.


RESULTS
Diagnosis criteria were established for 99mTc-anti-CD3 uptake in different diseases. RA and JIA showed joint uptake with progressive increase in late images. Gouty arthritis showed joint uptake with decrease during the late images. Joint uptake was low or absent in patients with OA, although when present the joint uptake decreased during the examination.


CONCLUSIONS
99mTc-anti-CD3 scintigraphy is a useful method in the differential diagnosis of rheumatic diseases.
Lopes
Flávia Paiva Proença Lobo
FP
Serviço de Medicina Nuclear, Departamento de Radiologia, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
de Azevedo
Mário Newton Leitão
MN
Marchiori
Edson
E
da Fonseca
Lea Mirian Barbosa
LM
de Souza
Sergio Augusto Lopes
SA
Gutfilen
Bianca
B
D017252:CD3 Complex / Q000276:immunology*; D003937:Diagnosis, Differential; D006801:Humans; D018796:Immunoconjugates; D007596:Joints; D016719:Radioimmunodetection / Q000379:methods*; D019275:Radiopharmaceuticals; D012216:Rheumatic Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000503:physiopathology; D012720:Severity of Illness Index; D013667:Technetium
D017252:CD3 Complex; D018796:Immunoconjugates; D019275:Radiopharmaceuticals; D013667:Technetium