Characterization of conserved CDR3 sequence of TCR alpha- and beta-chain genes in peripheral blood T-cells from patients with diffuse large B-cell lymphoma.
T-cell immunodeficiency is a common feature in cancer patients, but clonally expanded T cells which were considered to have the specific anti-tumor cytotoxicity can be identified in most cancer patients, including those with hematological malignancies. We previously reported that there were skewed usages of T-cell receptor (TCR) Valpha and TCR Vbeta subfamily in clonally expanded T cells in the peripheral blood (PB) of patients with diffuse large B-cell lymphoma (DLBCL). In order to investigate the characteristics of the complementarity determining region 3 (CDR3) of TCR alpha- and beta-chain genes in clonally expanded T cells, the sequences of TCR-CDR3 region of clonally expanded TCR Valpha and TCR Vbeta subfamily T cells were analyzed. Conserved amino acid motifs of TCR-CDR3 region were identified in different clonally expanded T cells. RSxYNTDKLI, SxGG, GGS, and DSxY motifs were found in the TCR Valpha subfamily T-cell clones, while SxGTG, SRG, and GTxD motifs existed in the TCR Vbeta subfamily T-cell clones. Moreover, the TCR harboring conserved motif in CDR3 region of some T-cell clones had high homology in the three-dimensional spatial structures. These findings revealed the mono/oligoclonally expanded T cells were derived from the stimulation of some antigens in the PB of patients with DLBCL, and may be recognizing an identical antigenic epitope.
Yin
Qingsong
Q
Institute of Hematology, Jinan University, Guangzhou, China.
Tan
Huo
H
Chen
Shaohua
S
Yang
Lijian
L
Ye
Jingmei
J
Li
Yangqiu
Y
D000369:Aged, 80 and over; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002999:Clone Cells / Q000737:chemistry; D022801:Complementarity Determining Regions / Q000235:genetics*; D017124:Conserved Sequence; D000939:Epitopes / Q000276:immunology; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012333:RNA, Messenger / Q000097:blood; D012334:RNA, Neoplasm / Q000097:blood; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016176:T-Lymphocyte Subsets / Q000737:chemistry*
D022801:Complementarity Determining Regions; D000939:Epitopes; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016693:Receptors, Antigen, T-Cell, alpha-beta