Expression patterns of FGF receptors in the developing mammalian cochlea.
Many studies have shown the importance of the fibroblast growth factor (FGF) family of factors in the development of the mammalian cochlea. There are four fibroblast growth factor receptors (FGFR1-4) and all four are expressed in the cochlea during development. While there are examples in the literature of expression patterns of some of the receptors at specific stages of cochlear development there has been no systematic study. We have assembled a full analysis of the patterns of receptor expression during cochlear development for all four Fgfrs using in situ hybridization. We have analyzed the expression patterns from embryonic day 13.5 through postnatal ages. We find that Fgfr1, 2, and 3 are expressed in the epithelium of the cochlear duct and Fgfr4 is limited in its expression to the mesenchyme surrounding the duct. We compare the receptor expression pattern to markers of the sensory domain (p27kip1) and the early hair cells (math1).
Department of Biological Structure, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98109, USA.
Toshinori
T
Hayashi
Catherine A
CA
Ray
Christa
C
Younkins
Olivia
O
Bermingham-McDonogh
D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D003053:Cochlear Duct / Q000196:embryology*; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000096:biosynthesis*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000096:biosynthesis*; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000096:biosynthesis*
D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors; C496349:Fgfr1 protein, mouse; C496354:Fgfr2 protein, mouse; C496366:Fgfr3 protein, mouse; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3