In vitro TAXOL production, by Pestalotiopsis breviseta--a first report.
Coelomycetous fungi were screened for the production of TAXOL. TAXOL production of Pestalotiopsis breviseta fungi is confirmed by Ultra Violet (UV) spectroscopic analysis, Infra Red (IR) analysis, high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and LC-MASS spectroscopy. TAXOL isolated from the P. breviseta fungus was identical with authentic TAXOL and produces 0.064 mg/L (0.128% dry weight of fungal mat).
Kathiravan
Govindarajan
G
Department of Biotechnology, Vels University, Pallavaram, Chennai 6OO 117, Tamilnadu, India. gkathir72@gmail.com
Sri Raman
Vithiyanathan
V
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013058:Mass Spectrometry; D017239:Paclitaxel / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D013057:Spectrum Analysis; D013098:Xylariales / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel