Measles virus M protein-driven particle production does not involve the endosomal sorting complex required for transport (ESCRT) system.
Assembly and budding of enveloped RNA viruses rely on viral matrix (M) proteins and host proteins involved in sorting and vesiculation of cellular cargoes, such as the endosomal sorting complex required for transport (ESCRT). The measles virus (MV) M protein promotes virus-like particle (VLP) production, and we now show that it shares association with detergent-resistant or tetraspanin-enriched membrane microdomains with ebolavirus VP40 protein, yet accumulates less efficiently at the plasma membrane. Unlike VP40, which recruits ESCRT components via its N-terminal late (L) domain and exploits them for particle production, the M protein does this independently of this pathway, as (i) ablation of motifs bearing similarity to canonical L domains did not affect VLP production, (ii) it did not redistribute Tsg101, AIP-1 or Vps4A to the plasma membrane, and (iii) neither VLP nor infectious virus production was sensitive to inhibition by dominant-negative Vps4A. Importantly, transfer of the VP40 L domain into the MV M protein did not cause recruitment of ESCRT proteins or confer sensitivity of VLP release to Vps4A, indicating that MV particle production occurs independently of and cannot be routed into an ESCRT-dependent pathway.
Institute for Virology and Immunobiology, University of Wuerzburg, Versbacher Str. 7, D-97078 Wuerzburg, Germany.
Andreas
A
Salditt
Susanne
S
Koethe
Christine
C
Pohl
Harry
H
Harms
Larissa
L
Kolesnikova
Stephan
S
Becker
Sibylle
S
Schneider-Schaulies
D002460:Cell Line; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D008459:Measles virus / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D019065:Virus Assembly; D057074:Virus Release
D056827:Endosomal Sorting Complexes Required for Transport; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins