A reason for overlap peaks in direct sequencing of rRNA gene ITS in Pleurotus nebrodensis.
To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis, we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.
State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing, China.
Chenyang
C
Huang
Jingyu
J
Xu
Wei
W
Gao
Qiang
Q
Chen
Hexiang
H
Wang
Jinxia
J
Zhang
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D054643:INDEL Mutation; D020076:Pleurotus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011523:Protoplasts; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer