Molecular epidemiology of Staphylococcus aureus in food samples based on the protein A gene polymorphic region DNA sequence.
Foodborne disease due to Staphylococcus aureus is a common and important disease worldwide. Molecular typing of S. aureus strains plays a crucial role in epidemiological studies examining the origin and performing surveillance of major infections. In this survey, we collected 913 food samples and detected 93 S. aureus isolates by using culture and biochemical tests. Subsequently, the X region of the protein A gene was amplified by the polymerase chain reaction (PCR) and amplicons were digested with HaeII and HindIII. Seven different patterns, ranging in length between 1200 and 1600 bp, showed gene polymorphism of the spa gene. The most prevalent spa types were D (20%) and C (16%) in dairy products and D (6%) and E (3%) in meat products. Consequently, 16 genotypes were obtained by HaeII digestion. Typeability of PCR - restriction fragment length polymorphism in genotyping of S. aureus strains in our study was perfect. Therefore, this method is a reliable, rapid, and powerful system in epidemiological investigations.
Microbiology Division, Pathobiology Department, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran. soltanirad34@yahoo.com
M M
MM
Soltan-Dallal
Z
Z
Salehipour
Jalil F
JF
Mehrabadi
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D005838:Genotype; D008460:Meat / Q000382:microbiology; D055624:Methicillin-Resistant Staphylococcus aureus; D017720:Molecular Epidemiology / Q000379:methods*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D013205:Staphylococcal Protein A