OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice.
Phosphate (Pi) homeostasis in plants is required for plant growth and development, and is achieved by the coordination of Pi acquisition, translocation from roots to shoots, and remobilization within plants. Previous reports have demonstrated that over-expression of OsPHR2 (the homolog of AtPHR1) and knockdown of OsSPX1 result in accumulation of excessive shoot Pi in rice. Here we report that OsPHR2 positively regulates the low-affinity Pi transporter gene OsPT2 by physical interaction and upstream regulation of OsPHO2 in roots. OsPT2 is responsible for most of the OsPHR2-mediated accumulation of excess shoot Pi. OsSPX1 suppresses the regulation on expression of OsPT2 by OsPHR2 and the accumulation of excess shoot Pi, but it does not suppress induction of OsPT2 or the accumulation of excessive shoot Pi in the Ospho2 mutant. Our data also show that OsSPX1 is a negative regulator of OsPHR2 and is involved in the feedback of Pi-signaling network in roots that is defined by OsPHR2 and OsPHO2. This finding provides new insight into the regulatory mechanism of Pi uptake, translocation, allocation and homeostasis in plants.
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Fang
F
Liu
Zhiye
Z
Wang
Hongyan
H
Ren
Chenjia
C
Shen
Ye
Y
Li
Hong-Qing
HQ
Ling
Changyin
C
Wu
Xingming
X
Lian
Ping
P
Wu
D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics
D004269:DNA, Bacterial; D010710:Phosphates; D010940:Plant Proteins; D018749:RNA, Plant; C036483:T-DNA