Eimeria tenella: effects of diclazuril treatment on microneme genes expression in second-generation merozoites and pathological changes of caeca in parasitized chickens.
The effects of diclazuril on mRNA expression levels of invasion-related microneme genes were examined in second-generation merozoites of Eimeria tenella (E. tenella) by quantitative real-time (QRT) PCR. Diclazruil treatment of infected chickens significantly decreased the number of second-generation merozoites by 65.13%, and resulted in downregulation of EtMIC genes: EtMIC1 by 65.63%, EtMIC2 by 64.12%, EtMIC3 by 56.82%, EtMIC4 by 73.48%, and EtMIC5 by 78.17%. SEM images of caecum tissue from uninfected chickens showed regular intestinal villus structure. In infected chickens, a distinct loss of the superficial epithelium, with a flattened mucosa and large-area necrosis and anabrosis, was evident. In diclazruil-treated chickens, a decrease in merozoite number and a visibly improved appearance of the caeca were noted. These improvements appeared to be mediated in part by downregulation of the expression of invasion-related EtMIC genes in response to diclazuril.
Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Bian-hua
BH
Zhou
Hong-wei
HW
Wang
Xiao-yang
XY
Wang
Li-fang
LF
Zhang
Ke-yu
KY
Zhang
Fei-qun
FQ
Xue
D000818:Animals; D002432:Cecum / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D003049:Coccidiostats / Q000494:pharmacology / Q000627:therapeutic use*; D018076:DNA, Complementary / Q000378:metabolism; D016786:Eimeria tenella / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D052940:Merozoites / Q000187:drug effects / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009570:Nitriles / Q000494:pharmacology / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000188:drug therapy* / Q000469:parasitology / Q000473:pathology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D011897:Random Allocation; D014227:Triazines / Q000494:pharmacology / Q000627:therapeutic use*
D003049:Coccidiostats; D018076:DNA, Complementary; D006023:Glycoproteins; D009570:Nitriles; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D014227:Triazines; C408742:microneme protein 5, Eimeria tenella; C057884:diclazuril