Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis.
L-Amino acid alpha-ligase (Lal), catalyzing the formation of alpha-dipeptides from unprotected L-amino acids in an ATP-dependent manner, is used in cost-effective fermentative production of dipeptides. We searched for novel Lals by in silico screening using Hidden Markov Model-based profile analysis, and identified five novel Lals that showed low similarity and different substrate specificity from known Lals.
Senoo
Akihiro
A
Bioprocess Development Center, Kyowa Hakko Bio Co., Ltd., Tsukuba, Ibaraki, Japan. akihiro.senoo@kyowa-kirin.co.jp
Tabata
Kazuhiko
K
Yonetani
Yoshiyuki
Y
Yagasaki
Makoto
M
D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology; D003198:Computer Simulation; D004151:Dipeptides / Q000096:biosynthesis* / Q000235:genetics; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008390:Markov Chains; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012995:Solubility; D013379:Substrate Specificity / Q000235:genetics
D000596:Amino Acids; D004151:Dipeptides; D009842:Oligopeptides; D011994:Recombinant Proteins; D008025:Ligases