Expression of somatostatin receptors in human melanoma cell lines: effect of two different somatostatin analogues, octreotide and SOM230, on cell proliferation.
Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was < 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.
Martinez-Alonso
M
M
Department of Basic Medical Sciences, Hospital Universitari Arnau de Vilanova, University of Lleida, IRB-Lleida, Lleida, Spain.
Llecha
N
N
Mayorga
M E
ME
Sorolla
A
A
Dolcet
X
X
Sanmartin
V
V
Abal
L
L
Casanova
J M
JM
Baradad
M
M
Yeramian
A
A
Egido
R
R
Puig
S
S
Vilella
R
R
Matias-Guiu
X
X
Marti
R M
RM
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D015282:Octreotide / Q000494:pharmacology*; D017481:Receptors, Somatostatin / Q000235:genetics* / Q000378:metabolism; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000494:pharmacology
D017481:Receptors, Somatostatin; D013004:Somatostatin; C517782:pasireotide; D015282:Octreotide