Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress.
We show that genomic hybridization allows detection of a spontaneous secondary deletion of 126 genes that occurred during construction of an Escherichia coli ytfE mutant, LMS4209, explaining some of its unexpected growth defects. We confirm that YtfE is required to repair damage to iron-sulfur centres and for hydrogen peroxide resistance.
Vine
Claire E
CE
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
Justino
Marta C
MC
Saraiva
Lígia M
LM
Cole
Jeffrey
J
D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007501:Iron / Q000378:metabolism; D009154:Mutation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017384:Sequence Deletion; D013455:Sulfur / Q000378:metabolism
D029968:Escherichia coli Proteins; C497162:YtfE protein, E coli; D013455:Sulfur; D006861:Hydrogen Peroxide; D007501:Iron