Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene.
Photo-excited bioactivities of fullerene derivatives are attracting much attention. In this report, a bis-methanophosphonate fullerene (BMPF) and the other two fullerene derivatives, a bis-malonic acid fullerene (BMAF) and a fullerol were incubated with HeLa cells and irradiated with a green light emitted from a mercury lamp on a fluorescent microscopy. By using DNA fluorescent probe propidium iodide staining method, damage towards cell membrane could be detected when cells were treated by irradiation altogether with BMPF or BMAF at a low concentration (4 microM), and the damage was dose-dependent. The activity of BMPF was much higher than that of BMAF, while fullerol had no effects under the same condition. It was also revealed that different kinds of reactive oxygen species (ROS) correlated to BMPF and BMAF. Additionally, presence of extracellular calcium could promote the activities of both derivatives, while removal of extracellular calcium could not abort their membrane-damaged activities. These results indicated that ROS and calcium were involved in the photosensitization of fullerene derivatives, and BMPF was a superior photosensitizer which would find potential application in biomedical field.
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Xinge
X
Qiao
Cheng
C
Huang
Yabing
Y
Ying
Xinlin
X
Yang
Yang
Y
Liu
Qiu
Q
Tian
D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects* / Q000648:ultrastructure; D037741:Fullerenes / Q000008:administration & dosage*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D037741:Fullerenes; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D002118:Calcium