A nonviral minicircle vector for deriving human iPS cells.
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
Jia
Fangjun
F
Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.
Wilson
Kitchener D
KD
Sun
Ning
N
Gupta
Deepak M
DM
Huang
Mei
M
Li
Zongjin
Z
Panetta
Nicholas J
NJ
Chen
Zhi Ying
ZY
Robbins
Robert C
RC
Kay
Mark A
MA
Longaker
Michael T
MT
Wu
Joseph C
JC
D000328:Adult; D004270:DNA, Circular / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D014162:Transfection
D004270:DNA, Circular