The temporal, PFGE and resistance pattern associations suggest that poultry products are only a minor source of human infections in western Finland.
In order to compare human and retail poultry meat thermophilic Campylobacter isolates originating in a regional area in Western Finland, minimum inhibitory concentration (MICs) for six antimicrobials (96 isolates) and pulsed-field gel electrophoresis (PFGE) (102 isolates) were analysed. Campylobacter spp. were detected in 10.5% out of 305 fresh poultry products studied; 29 (90.5%) isolates were identified as Campylobacter jejuni. Among the 70 human isolates, 66 (94.3%) isolates were identified as C. jejuni. Only one C. jejuni domestic poultry isolate showed resistance (ampicillin), whereas domestic human C. jejuni isolates were more commonly resistant to ciprofloxacin, nalidixic acid, ampicillin and tetracycline. The resistance in foreign human isolates was significantly more common than among domestic isolates. PFGE analysis with KpnI restriction enzyme resulted in 59 different PFGE types among the poultry and human isolates. Three types were detected first in poultry meat and thereafter during the following month in domestic human samples, whereas the other conjoint types were detected only after many months. This study suggests that poultry products play only a minor role in human campylobacteriosis in the study area and that the resistance found in domestic human isolates is not likely related to retail poultry meat products.
Lyhs
Ulrike
U
Ruralia Institute, Seinäjoki unit, University of Helsinki, Kampusranta 9C, FI-60320 Seinäjoki, Finland. ulrike.lyhs@helsinki.fi <ulrike.lyhs@helsinki.fi>
Katzav
Marianne
M
Isohanni
Pauliina
P
Heiska
Helmi
H
Maijala
Riitta
R
D000818:Animals; D002169:Campylobacter Infections / Q000209:etiology* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005387:Finland; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D008461:Meat Products / Q000382:microbiology*; D011200:Poultry / Q000382:microbiology*