Use of polystyrene-supported 2-isobutoxy-1-isobutoxycarbonyl-1,2-dihydroquinoline for the preparation of a hapten-protein conjugate for antibody development.
Polystyrene-supported 2-isobutoxy-1-isobutoxycarbonyl-1,2-dihydroquinoline (PS-IIDQ), a polymer-supported covalent coupling reagent, was successfully employed for the first time in the bioconjugation of an example hapten (phytanic acid derivative) to a carrier protein (bovine serum albumin (BSA)) within the context of immunogen preparation for antibody development. The ability of the prepared example phytanic acid derivative-BSA conjugate to bind an anti-phytanic acid antibody was confirmed using an enzyme-linked immunosorbent assay (ELISA).
Sathe
Manisha
M
Cranfield Health, Cranfield University, Cranfield, Bedfordshire MK43 0AL, United Kingdom.
Derveni
Mariliza
M
Allen
Marjorie
M
Cullen
David C
DC
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D010831:Phytanic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D011137:Polystyrenes / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000906:Antibodies; D006241:Haptens; D011137:Polystyrenes; D011804:Quinolines; D010831:Phytanic Acid; D012710:Serum Albumin, Bovine; C026101:IIDQ