Baicalin attenuates oxygen-glucose deprivation-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase activation in PC12 cells.
OBJECTIVE
To determine whether the flavonoid baicalin attenuates oxygen-glucose deprivation (OGD)-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase (5-LOX) activation in PC12 cells.


METHODS
The effects of baicalin and the 5-LOX inhibitor zileuton on the changes induced by OGD/recovery or H(2)O(2) (an exogenous reactive oxygen species [ROS]) in green fluorescent protein-5-LOX-transfected PC12 cells were compared.


RESULTS
Both baicalin and zileuton attenuated OGD/recovery- and H(2)O(2)-induced injury and inhibited OGD/recovery-induced production of 5-LOX metabolites (cysteinyl leukotrienes) in a concentration-dependent manner. However, baicalin did not reduce baseline cysteinyl leukotriene levels. Baicalin also reduced OGD/recovery-induced ROS production and inhibited 5-LOX translocation to the nuclear envelope and p38 phosphorylation induced by OGD/recovery and H(2)O(2). In contrast, zileuton did not show these effects.


CONCLUSIONS
Baicalin can inhibit 5-LOX activation after ischemic injury, which may partly result from inhibition of the ROS/p38 mitogen-activated protein kinase pathway.
Department of Pharmacology, Zhejiang University, Hangzhou, China.
Cheng-tan
CT
Li
Wei-ping
WP
Zhang
San-hua
SH
Fang
Yun-bi
YB
Lu
Li-hui
LH
Zhang
Ling-ling
LL
Qi
Xue-qin
XQ
Huang
Xiao-jia
XJ
Huang
Er-qing
EQ
Wei
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D004789:Enzyme Activation; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D005419:Flavonoids; D017382:Reactive Oxygen Species; C038044:baicalin; D006861:Hydrogen Peroxide; D001094:Arachidonate 5-Lipoxygenase; D048051:p38 Mitogen-Activated Protein Kinases; D005947:Glucose; D010100:Oxygen