Microinjection of dsRNA into mouse one-cell embryos.
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing in a number of model systems. The following protocol describes delivery of a double-stranded RNA (dsRNA) of choice into mouse one-cell embryos by microinjection. Microinjected embryos can be cultured for up to 4 d, i.e., to the blastocyst stage. The efficiency of knockdown by RNAi can be assayed by quantitative reverse transcriptase (RT)-PCR (qPCR).
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. steinpau@sas.upenn.edu
Paula
P
Stein
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D020868:Gene Silencing; D005821:Genetic Techniques; D008297:Male; D051379:Mice; D008845:Microinjections / Q000379:methods*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012330:RNA, Double-Stranded