Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body.
OBJECTIVE
To investigate the relationships among antioxidant activities, oxidative stress, and vascular endothelial growth factor (VEGF) in the vitreous body and serum from proliferative diabetic retinopathy (PDR) patients.


METHODS
In 21 patients with PDR and 21 controls with macular hole (MH), the VEGF and lipid peroxide (Nepsilon-hexanoyl-lysine [HEL]) levels in the vitreous and serum were measured by enzyme-linked immunosorbent assay, while antioxidant capacity (potential antioxidant [PAO]) was measured by chemical reduction of Cu(2+).


RESULTS
Both the PAO and HEL levels in the vitreous and serum were significantly higher in PDR patients than in those with MH (both p<0.01). The VEGF concentrations in the vitreous were higher in PDR patients than in those with MH (p<0.01); however, the VEGF concentrations in the serum were not different between the two groups (p=0.95). Positive correlations were found between the PAO and VEGF concentrations and between the HEL and VEGF concentrations in the vitreous of both the PDR and the MH patients.


CONCLUSIONS
Our study revealed that the PAO, HEL, and VEGF concentrations in the vitreous were increased in PDR versus MH patients and that there were positive correlations among these factors. This is consistent with VEGF and lipid peroxide levels in the vitreous playing some role in the pathogenesis of PDR.
Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, Gifu, Japan.
Hiroshi
H
Izuta
Nozomu
N
Matsunaga
Masamitsu
M
Shimazawa
Tetsuya
T
Sugiyama
Tsunehiko
T
Ikeda
Hideaki
H
Hara
D000975:Antioxidants / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D008239:Lysine / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012167:Retinal Perforations / Q000097:blood / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism* / Q000473:pathology
D000975:Antioxidants; C120589:N(epsilon)-hexanoyllysine; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D008239:Lysine