Anti-inflammatory effects of gomisin N, gomisin J, and schisandrin C isolated from the fruit of Schisandra chinensis.
Schiandra chinensis is a well-known Chinese traditional medicine for the treatment of hepatic disease. In this study, we investigated whether the nine major compounds of Schiandra chinensis could be applied to suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Among the nine lignans, three, gomisin J, gomisin N, and schisandrin C, were found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. These three lignans showed low cytotoxic effects in Raw 264.7 cells. Pre-treatment of Raw 264.7 cells with gomisin J, gomisin N, or schisandrin C reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to be caused by blockage of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and c-Jun N-terminal kinase (JNK) phosphorylation.
Oh
Su-Yeon
SY
Department of Biology Education, College of Education, Chungbuk National University, Chungcheongbuk-Do, Republic of Korea.
Kim
Young Hoon
YH
Bae
Deok Sung
DS
Um
Byung Hun
BH
Pan
Cheol-Ho
CH
Kim
Chul Young
CY
Lee
Hee Ju
HJ
Lee
Jae Kwon
JK
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D034242:Cyclooctanes / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000378:metabolism; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011083:Polycyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D032141:Schisandra / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C015499:schizandrin B; C031409:schizandrin C; C082091:gomisin J