Displacement chromatography of proteins.
This unit discusses the important parameters in designing and optimizing a separation by ion-exchange displacement chromatography, including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for testing a column, determining binding and elution conditions, displacing the sample, and cleaning, regenerating, and storing of displacement columns.
McAtee
C Patrick
CP
SACHEM, Inc., Austin, Texas, USA.
D002021:Buffers; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D007475:Ion Exchange Resins; D011506:Proteins / Q000302:isolation & purification*
D002021:Buffers; D007475:Ion Exchange Resins; D011506:Proteins