Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog.
A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)).
Bräuer
Suzanna L
SL
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Cadillo-Quiroz
Hinsby
H
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Ward
Rebekah J
RJ
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Yavitt
Joseph B
JB
Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA.
Zinder
Stephen H
SH
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
D002245:Carbon Dioxide / Q000378:metabolism; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism; D017018:Methanomicrobiales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012987:Soil; D013696:Temperature; D014481:United States; D053833:Wetlands
D003470:Culture Media; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002245:Carbon Dioxide; D012965:Sodium Chloride; D006859:Hydrogen; D008697:Methane