Expression of Epiplakin1 in the developing and adult mouse retina.
OBJECTIVE
To assess the expression of Epiplakin1 (Eppk1) in the developing and adult mouse retina.


METHODS
Eppk1 expression was examined using reverse transcription-polymerase chain reaction (RTPCR) analysis and immunoblot analysis. The Eppk1 expression pattern was examined by immunohistochemical analysis in embryonic and adult C57BL/6J mice.


RESULTS
Both Eppk1 mRNA and protein were detected in adult mouse retina. Eppk1 was expressed in nestin-positive neural progenitor cells in the embryonic retina. In adults, Eppk1 was expressed in retinal ganglion cells, bipolar cells, and Müller glial cells.


CONCLUSIONS
These results suggest that Eppk1 is expressed both in embryonic and adult retina and may be involved in retinal development.
Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo, Japan.
Tetsu
T
Yoshida
Xiaoli
X
Guo
Kazuhiko
K
Namekata
Yoshinori
Y
Mitamura
Shoen
S
Kume
Takayuki
T
Harada
D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D005260:Female; D047109:Fetal Development; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000196:embryology* / Q000378:metabolism*; D051245:Retinal Bipolar Cells / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001324:Autoantigens; D012333:RNA, Messenger; C426678:epiplakin; D001973:Bromodeoxyuridine