Multi-walled carbon nanotubes affect drug transport across cell membrane in rat astrocytes.
The impact of carbon nanotubes on the cell membrane is an aspect of particular importance and interest in the study of carbon nanotubes' interactions with living systems. One of the many functions of the cell membrane is to execute substance transport into and out of the cell. We investigated the influence of multi-walled carbon nanotubes (MWCNTs) on the transport of several compounds across in the cell membrane of rat astrocytes using flow cytometry. These compounds are fluorescein diacetate, carboxyfluorescein diacetate, rhodamine 123 and doxorubicin, which are prosubstrate/substrates of multidrug transporter proteins. Results showed that MWCNTs significantly inhibited cellular uptake of doxorubicin but not the other drugs and the mode of loading made a significant difference in doxorubicin uptake. Retention of fluorescein, carboxyfluorescein and rhodamine 123 was remarkably higher in MWCNT-exposed cells after an efflux period. A kinetics study also demonstrated slower efflux of intracellular fluorescein and rhodamine 123. Data presented in this paper suggest that MWCNTs could affect drug transport across cell membranes. The implications of the findings are discussed.
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, 430030 Wuhan, People's Republic of China. mornsmile@yahoo.com
Xiao
X
Chen
Hermann J
HJ
Schluesener
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004317:Doxorubicin / Q000378:metabolism; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D007700:Kinetics; D036103:Nanotechnology; D037742:Nanotubes, Carbon; D051381:Rats; D020112:Rhodamine 123 / Q000378:metabolism
D005452:Fluoresceins; D037742:Nanotubes, Carbon; C027780:carboxyfluoresceindiacetate; D020112:Rhodamine 123; D004317:Doxorubicin; C018506:diacetylfluorescein