Molding a silver nanoparticle template on polydimethylsiloxane to efficiently capture mammalian cells.
Herein, a functional template made up of in situ synthesized silver nanoparticles (AgNPs) is prepared on polydimethylsiloxane (PDMS) for the spatial control of cell capture, where the residual Si-H groups in the PDMS matrix are used as reductants to reduce AgNO(3) for forming AgNPs. In virtue of microfluidic system, a one-dimensional array pattern of AgNPs is obtained easily. Further combining with plasma treatment, a two-dimensional array pattern of AgNPs could be achieved. The obtained PDMS-AgNPs composite is characterized in detail. The PDMS-AgNPs composite shows good antibacterial property in E. coli adhesion tests. The patterns possess hifi and high resolution (ca. 8 microm). Cell patterns with high efficiency and spatial selectivity are further formed with the aid of H-Arg-Gly-Asp-Cys-OH (RGDC) tetrapeptide which is grafted on the AgNPs template. Cells immobilized on the template show a good ability for adhesion, spreading, migration, and growth.
Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Hai-Jing
HJ
Bai
Hong-Lei
HL
Gou
Jing-Juan
JJ
Xu
Hong-Yuan
HY
Chen
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D046210:Microfluidic Analytical Techniques; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; D012834:Silver; C013830:baysilon