Fixing and sectioning tissue from the plant Kalanchoë daigremontiana.
Tissue samples from Kalanchoë daigremontiana can be fixed for scanning electron microscopy (SEM), histology, and in situ hybridization using several common steps described in this protocol. All steps should be performed in the same manner for all three methods unless otherwise noted.
Garcês
Helena
H
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Sinha
Neelima
N
D001901:Botany / Q000379:methods; D017403:In Situ Hybridization; D031962:Kalanchoe / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D059828:Plant Cells; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods*