Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples.
A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
Gomes
Luciana I
LI
Laboratório de Pesquisas Clínicas, Instituto de Pesquisas René Rachou-Fiocruz, Av Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil.
Marques
Letícia H S
LH
Enk
Martin J
MJ
Coelho
Paulo Marcos Z
PM
Rabello
Ana
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D016054:DNA, Protozoan / Q000032:analysis*; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000235:genetics* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D055815:Young Adult
D016054:DNA, Protozoan