Environmental determinants of and impact on childhood asthma by the bacterial community in household dust.
Asthma increased dramatically in the last decades of the 20th century and is representative of chronic diseases that have been linked to altered microbial exposure and immune responses. Here we evaluate the effects of environmental exposures typically associated with asthma protection or risk on the microbial community structure of household dust (dogs, cats, and day care). PCR-denaturing gradient gel analysis (PCR-DGGE) demonstrated that the bacterial community structure in house dust is significantly impacted by the presence of dogs or cats in the home (P = 0.0190 and 0.0029, respectively) and by whether or not children attend day care (P = 0.0037). In addition, significant differences in the dust bacterial community were associated with asthma outcomes in young children, including wheezing (P = 0.0103) and specific IgE (P = 0.0184). Our findings suggest that specific bacterial populations within the community are associated with either risk or protection from asthma.
Department of Soil, Water and Environmental Science, University of Arizona, 429 Shantz Building #38, Tucson, AZ 85721, USA. rmaier@ag.arizona.edu
Raina M
RM
Maier
Michael W
MW
Palmer
Gary L
GL
Andersen
Marilyn J
MJ
Halonen
Karen C
KC
Josephson
Robert S
RS
Maier
Fernando D
FD
Martinez
Julia W
JW
Neilson
Debra A
DA
Stern
Donata
D
Vercelli
Anne L
AL
Wright
D000818:Animals; D001249:Asthma / Q000453:epidemiology* / Q000473:pathology; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D004391:Dust; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure; D006801:Humans; D009691:Nucleic Acid Denaturation; D012307:Risk Factors
D004269:DNA, Bacterial; D004391:Dust