Labisia pumila extract protects skin cells from photoaging caused by UVB irradiation.
Labisia pumila (Myrsinaceae), known as "Kacip Fatimah," has been used by many generations of Malay women to induce and facilitate child birth as well as a post partum medicine. However, its topical application on skin has not been reported yet. In this study, we have focused on the anti-photoaging effects of L. pumila. Extract of L. pumila was first analyzed for their antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) since UV irradiation is a primary cause of reactive oxygen species (ROS) generation in the skin. The 50% free radical scavenging activity (FSC(50)) of L. pumila extract was determined to be 0.006%, which was equal to that produced by 156 microM ascorbic acid. TNF-alpha and cyclooxygenase (COX-2) play a primary role in the inflammation process upon UV irradiation and are known to be stimulated by UVB. Treatment with L. pumila extract markedly inhibited the TNF-alpha production and the expression of COX-2. Decreased collagen synthesis of human fibroblasts by UVB was restored back to normal level after treatment with L. pumila extract. On the other hand, the enhanced MMP-1 expression upon UVB irradiation was down regulated by L. pumila extract in a dose-dependent manner. Furthermore, treatment of normal keratinocytes with L. pumila extract attenuated UVB-induced MMP-9 expression. These results collectively suggest L. pumila extract has tremendous potential as an anti-photoaging cosmetic ingredient.
Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul 100-715, South Korea.
Hyun-kyung
HK
Choi
Dong-hyun
DH
Kim
Jin Wook
JW
Kim
Sulaiman
S
Ngadiran
Mohamad Roji
MR
Sarmidi
Chang Seo
CS
Park
D000375:Aging / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects; D010936:Plant Extracts / Q000008:administration & dosage*; D029623:Primulaceae / Q000737:chemistry*; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D015595:Skin Aging / Q000502:physiology* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D016207:Cytokines; D010936:Plant Extracts; D011837:Radiation-Protective Agents