Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts.
The transitional behaviors of myoblasts toward differentiation were investigated in the cultures at the low and high seeding densities (respectively, X(0)=1.0x10(3) and 2.0x10(5) cells/cm(2)). In the culture at the low seeding density, an increase in confluence degree accompanied a decrease in growth potential (R(p)), being R(p)=0.85 and 0.11 at t=48 and 672 h, respectively. Myoblasts seeded at the high density resulted in the immediate cessation of growth with keeping the low range of R(p)=0.02-0.09 throughout the culture. The reduction of R(p) led to the generation of three subpopulations of cells in proliferative, quiescent and differentiated states. Close cell contacts in the confluent state of high seeding culture induced cell quiescence to a higher extent with suppressing differentiation.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Shiplu Roy
SR
Chowdhury
Yuichi
Y
Muneyuki
Yasunori
Y
Takezawa
Masahiro
M
Kino-oka
Atsuhiro
A
Saito
Yoshiki
Y
Sawa
Masahito
M
Taya
D002450:Cell Communication / Q000502:physiology*; D002452:Cell Count; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*