In situ hybridization for metalloproteinases and their inhibitors.
In situ hybridization (ISH) is an invaluable tool in understanding tissue-specific gene expression and gene regulation within a spatial context and at a resolution that is not possible by any other method. In this chapter, we provide ISH methodology that has successfully been applied to the detection of metalloproteinases and their inhibitors.
Clinical Research Center, Oulu University Hospital, Oulu, Finland.
Tiina L
TL
Hurskainen
Suneel S
SS
Apte
D000818:Animals; D011232:Chemical Precipitation; D016616:Cryoultramicrotomy; D018076:DNA, Complementary / Q000235:genetics; D004076:Digoxigenin / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D000431:Ethanol; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D015347:RNA Probes / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013194:Staining and Labeling; D013698:Templates, Genetic; D016707:Tissue Fixation; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D015347:RNA Probes; D000431:Ethanol; D012260:Ribonucleases; D045726:Metalloproteases; D004076:Digoxigenin