Application of gel permeation HPLC for lipoprotein profiling in dogs.
A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.
Mizutani
Hisashi
H
Veterinary Internal Medicine, Nippon Veterinary and Life Science University, Japan. hisashi-m@nvlu.ac.jp
Sako
Toshinori
T
Arai
Noriaki
N
Kuriyama
Koji
K
Yoshimura
Itaru
I
Mori
Akihiro
A
Iwase
Kenji
K
Hirose
Hisashi
H
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification; D008079:Lipoproteins, VLDL / Q000097:blood / Q000302:isolation & purification; D012016:Reference Values; D014280:Triglycerides / Q000097:blood / Q000302:isolation & purification; D014461:Ultracentrifugation / Q000379:methods
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides