Role of activating transcription factor 3 (ATF3) in sublytic C5b-9-induced glomerular mesangial cell apoptosis.
Sublytic complement C5b-9 complexes can cause cell apoptosis, but the mechanism of glomerular mesangial cell (GMC) apoptosis mediated by these complexes has not been well defined. The activating transcription factor 3 (ATF3) gene is an immediate early gene for the cell to cope with a variety of stress signals and can promote apoptosis of some cells. In this study, ATF3 expression and cell apoptosis in GMCs induced by sublytic C5b-9 were measured, and then the effects of ATF3 gene over-expression or knockdown on GMC apoptosis induced by sublytic C5b-9 were examined at a fixed time. The results showed that both ATF3 expression and GMC apoptosis were markedly increased and ATF3 over-expression obviously increased sublytic C5b-9-induced GMC apoptosis, whereas ATF3 gene silencing had a significant opposite effect. Collectively, these findings indicate that upregulation of ATF3 gene expression is involved in regulating GMC apoptosis induced by sublytic C5b-9 complexes.
Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China.
Xiaoming
X
Jiang
Jing
J
Zhang
Mei
M
Xia
Wen
W
Qiu
Hui
H
Wang
Dan
D
Zhao
Yingwei
Y
Wang
D051700:Activating Transcription Factor 3 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D015938:Complement Membrane Attack Complex / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D050527:Mesangial Cells / Q000166:cytology* / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats
D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D015938:Complement Membrane Attack Complex; D034741:RNA, Small Interfering