Influence of the solvent quality on the AhR mediated Procept assay measurement of dioxin and dioxin-like compounds.
Polychlorodibenzo-p-dioxins, polychorodibenzofurans and "dioxin-like" polychlorinated biphenyls are widespread persistent organic pollutants sharing a similar toxicological pathway mediated by the aryl-hydrocarbon receptor (AhR). Since the confirmatory method for their measurement at trace levels in complex matrices (using isotopic dilution and gas chromatography-high resolution mass spectrometry) remains time and cost-consuming, growing efforts of the scientific community have been focused on the development of screening approaches, including AhR mediated assays. Unfortunately, AhR ligands are highly diverse and agonistic/antagonistic effects can be observed on procedural blanks and/or sample extracts. In this study, the influence of solvent grade quality on the response of a DNA-binding AhR mediated assay used for screening dioxins has been investigated. Our results demonstrated a very critical impact of this parameter with both strong agonistic and antagonistic effects observed for any tested solvent lot. A small silver nitrate silica column removed partly these interfering compounds and then can be recommended as final purification step. Some preferable grades can be identified and selected in order to guarantee the best possible performances. However, it appears necessary to test every new lot, even if a grade appeared previously compliant.
Cariou
Ronan
R
LABoratoire d'Etude des Résidus et Contaminants dans les Aliments, Ecole Nationale Vétérinaire de Nantes, USC INRA 2013, BP 50707, 44307 Nantes Cedex 3, France. laberca@vet-nantes.fr
McAlister
Daniel R
DR
Marchand
Philippe
P
Fern
Michael J
MJ
Antignac
Jean-Philippe
JP
Le Bizec
Bruno
B
D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D004247:DNA / Q000378:metabolism*; D004147:Dioxins / Q000032:analysis*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014050:Toluene / Q000737:chemistry*
D002273:Carcinogens; D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon; D014050:Toluene; D004247:DNA