Review of fructosyl amino acid oxidase engineering research: a glimpse into the future of hemoglobin A1c biosensing.
Glycated proteins, particularly glycated hemoglobin A1c, are important markers for assessing the effectiveness of diabetes treatment. Convenient and reproducible assay systems based on the enzyme fructosyl amino acid oxidase (FAOD) have become attractive alternatives to conventional detection methods. We review the available FAOD-based assays for measurement of glycated proteins as well as the recent advances and future direction of FAOD research. Future research is expected to lead to the next generation of convenient, simple, and economical sensors for glycated protein, ideally suited for point-of-care treatment and self-monitoring applications.
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
Stefano
S
Ferri
Seungsu
S
Kim
Wakako
W
Tsugawa
Koji
K
Sode
D000594:Amino Acid Oxidoreductases; D057005:Bioengineering / Q000639:trends; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans
D000594:Amino Acid Oxidoreductases; D001786:Blood Glucose; D006442:Glycated Hemoglobin; C103012:amadoriase