The in vivo performance of polyvinyl alcohol macro-encapsulated islets.
Islet transplantation is a method for the treatment of type 1 diabetes mellitus (DM) and has been widely performed around the world. The long-term cryopreservation of islets shows many advantages in the field of islet transplantation. Previous studies have described the development of sheet-type polyvinyl alcohol (PVA) macro-encapsulated islets (MEI) to treat type 1 DM without any immunotherapy. The present study examined their beneficial effects on islet cryopreservation. PVA MEI of Wistar rats were divided into three groups of 1-day, 7-day and 30-day cryopreservation at -80 degrees C. The 30-day group showed a lower recovery rate of the islet number and impaired insulin release in comparison to the 1-day group, whereas no significant differences of the in vitro results were observed between the 1-day and 7-day groups. The MEI transplantation recipient mice in the 1-day and 7-day groups reached normoglycemia for a 4-week observation period, and the recipients in 30-day group also showed a significant decrease followed by a slightly higher non-fasting blood glucose level. These results suggest that the PVA MEI are useful for islet long-term cryopreservation, and that the use of cryopreserved PVA MEI may, therefore, be a promising modality for performing DM therapy.
Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
Zhi
Z
Qi
Yanna
Y
Shen
Goichi
G
Yanai
Kaichiang
K
Yang
Yasumasa
Y
Shirouzu
Akihito
A
Hiura
Shoichiro
S
Sumi
D000818:Animals; D002096:C-Peptide / Q000097:blood; D015925:Cryopreservation / Q000379:methods*; D005951:Glucose Tolerance Test; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000378:metabolism* / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011142:Polyvinyl Alcohol / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous
D002096:C-Peptide; D007328:Insulin; D011142:Polyvinyl Alcohol