Electrokinetic concentration of DNA polymers in nanofluidic channels.
DNA molecules can be concentrated in a narrow region of a nanochannel when driven electrokinetically in submillimolar salt solutions. Transport experiments and theoretical modeling reveal the interplay of electrophoresis, electro-osmosis, and the unique statistical properties of confined polymers that lead to DNA aggregation. A finite conductance through the bulk of the device also plays a crucial role by influencing the electric fields in the nanochannel. We build on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA at selected locations and at physiological salt concentrations that are relevant to lab-on-a-chip applications.
Kavli Institute of NanoScience, Delft University of Technology, Delft, The Netherlands.
Derek
D
Stein
Zeno
Z
Deurvorst
Frank H J
FH
van der Heyden
Wiepke J A
WJ
Koopmans
Alan
A
Gabel
Cees
C
Dekker
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D008956:Models, Chemical; D009038:Motion; D049329:Nanostructures / Q000737:chemistry*
D004247:DNA