Production of tropane alkaloids during de-differentiation of Scopolia parviflora calli.
The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6beta-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.
Kim
Yong Duck
YD
Institute of Hadong Green Tea, Hadong, Gyeongnam, 667-804, South Korea, Division of Forest Research, Gyeongsangnam-do Forest Environment Research Institute, Jinju, Gyeongnam, 660-870, South Korea.
Kang
Seung Mi
SM
Min
Ji Yun
JY
Choi
Won Kyun
WK
Jeong
Mi Jin
MJ
Karigar
Chandrakant S
CS
Choi
Myung Suk
MS
D007723:Korea; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D012601:Scopolamine / Q000096:biosynthesis*; D032603:Scopolia / Q000737:chemistry* / Q000254:growth & development; D014326:Tropanes / Q000737:chemistry / Q000302:isolation & purification*
D014326:Tropanes; D012601:Scopolamine