One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase.
The newly established difructose anhydride IV (DFA IV) production system is comprised of the effective production of levan from sucrose by Serratia levanicum NN, the conversion of the levan into DFA IV by levan fructotransferase from Arthrobacter nicotinovorans GS-9, which is highly expressed in an Escherichiacoli transformant, and a practical purification step. The chemical properties of DFA IV were also investigated.
Kikuchi
Hiroto
H
Research Center, Nippon Beet Sugar Mfg. Co., Ltd., 9-13, Inada-cho, Obihiro, Hokkaido 080-0831, Japan.
Sakurai
Hiroaki
H
Nagura
Taizo
T
Aritsuka
Tsutomu
T
Tomita
Fusao
F
Yokota
Atsushi
A
D001173:Arthrobacter / Q000201:enzymology*; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry; D005630:Fructans / Q000737:chemistry* / Q000378:metabolism*; D006602:Hexosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D012705:Serratia / Q000378:metabolism*
D004187:Disaccharides; D005630:Fructans; C033869:difructose anhydride IV; C072599:levan; D006602:Hexosyltransferases; C040074:levan fructotransferase