Molecular cloning, characterization and expression of cDNA encoding translationally controlled tumor protein (TCTP) from Jatropha curcas L.
A cDNA encoding translationally controlled tumor protein (TCTP) of Jatropha curcas L., JcTCTP, was isolated from an endosperm cDNA library. JcTCTP consisted of a 5' untranslated region (UTR) of 526 bp, a 3' UTR of 377 bp and an open reading frame (ORF) of 507 bp, encoding a protein of 168 amino acid residues, which contained two signature sequences of TCTP family. Its deduced amino acid sequence was similar to the other known plants TCTPs in a range of 77.4-92.3%. Expression of JcTCTP was the highest in the stem, endosperm at embryo formation stage and embryo of J. curcas tissues, and the lowest in the endosperm at seminal leaf embryo stage and flower, demonstrating a pattern of temporal and spatial specific expression.
Qin
Xiaobo
X
College of Life Sciences, Sichuan University, 610064 Chengdu, China.
Gao
Fan
F
Zhang
Jinping
J
Gao
Jihai
J
Lin
Sha
S
Wang
Yong
Y
Jiang
Luding
L
Liao
Yi
Y
Wang
Lina
L
Jia
Yongjiong
Y
Tang
Lin
L
Xu
Ying
Y
Chen
Fang
F
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism*; D031286:Jatropha / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D000090324:Tumor Protein, Translationally-Controlled 1
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D010940:Plant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1