[Significance and expression of S100A9 and NMP238 in cervical carcinoma tissues with different concurrent chemoradiotherapy sensitivities].
OBJECTIVE
To determine the significance and expression of S100A9 and NMP238 in cervical carcinoma with different concurrent chemoradiotherapy sensitivities.


METHODS
Fresh carcinoma tissues were collected from untreated cervical carcinoma patients and preserved at -80 degree. The tissues were classified into 2 groups: a high sensitivity group (HS) and a low sensitivity group (LS) according to their response to concurrent chemoradiotherapy. Protein was separated by 2-dimensional gel electrophoresis (2-DE). Peptide mass fingerprintings (PMF) were acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascot-database. Differential expressed proteins were assayed by Western blot and immunohistochemistry.


RESULTS
Most of the gels were clear and were successfully and reproductively analyzed. Intensity and rate of S100A9 expression were higher in the HS group than in the LS group,and those of NMP238 expression were higher in the LS group than in the HS group.


CONCLUSIONS
S100A9 and NMP238 expression is associated with concurrent chemoradiotherapy sensitivity in cervical carcinoma.
Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China.
Hong
H
Zhu
Shan
S
Zeng
Liang
L
Zeng
Xuewei
X
Wang
Zhiqiang
Z
Xiao
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D040502:Calgranulin B / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000628:therapy; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000532:radiotherapy / Q000628:therapy*; D055815:Young Adult
D040502:Calgranulin B; D009687:Nuclear Proteins; C115808:nuclear matrix protein 238