[Study on the transmission of drug resistant human immunodeficiency virus-1 in Henan province].
OBJECTIVE
To survey the prevalence of HIV-1 drug resistance after five years antiretroviral treatment in Henan province.


METHODS
Through the cross-sectional study, serum specimens of 69 HIV infected individuals that were 2 to 25 years old who were newly diagnosed according the WHO standard from November 2007 to August 2008 and did not receive antiretroviral treatment (ART) were collected. HIV-1 pol genetic mutations associated with drug resistance were identified with RT-PCR and interpreted.


RESULTS
Out of 69 samples, 50 samples were successfully amplified and sequenced. Seven drug resistant mutation in reverse transcriptase region were detected and three mutations in protein region. In one specimen, a mutation (K103N) in reverse transcriptase was identified which caused high level resistance to NNRTIs, but no proteinase inhibitor mutation was found in protein region. According to the sampling and threshold surveillance criteria, the prevalence of drug resistant HIV-1 in Henan was less than 5%.


CONCLUSIONS
The prevalence of drug resistant HIV-1 was still at low level in Henan. However, the proportion of resistant strains would be higher with the antiretroviral treatment. We should pay more attention to the transmission of resistant strains and continue the drug resistance surveillance.
Yuan
Yuan
Y
Center for AIDS/STD Control and Prevention, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
Cao
Xin-liang
XL
Liu
Hong-wei
HW
Xing
Hui
H
Liu
Chun-hua
CH
Cui
Wei-guo
WG
Sun
Ding-yong
DY
Li
Ning
N
Zhu
Qian
Q
Wang
Zhe
Z
Shao
Yi-ming
YM
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D000293:Adolescent; D000328:Adult; D019380:Anti-HIV Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D002681:China; D003430:Cross-Sectional Studies; D024882:Drug Resistance, Viral / Q000187:drug effects / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D009154:Mutation; D055815:Young Adult
D019380:Anti-HIV Agents