Effects of cerebral microvascular endothelial cells and vascular endothelial growth factor on the proliferation and differentiation of NSCs: a comparative study.
In adult mammalian brain, microvessels may produce a special microenvironment and then influence the characteristics of neural stem cells (NSCs). This work aims to compare the effects of cerebral microvascular endothelial cells (CMECs) and vascular endothelial growth factor (VEGF) on the behaviors of NSCs. An in vitro model with co-cultured CMECs and NSCs was established by using transwell co-culture system. The expression of nestin and NF in the early stage of the co-culture, and NF in the later stage were explored by immunostaining. Results demonstrate that both CMECs and VEGF stimulate the proliferation of NSCs and inhibit the differentiation. After removing CMECs or VEGF from the co-culture system, a further culture in medium for the differentiation of NSCs was performed. The percentage of NF-positive cells in the CMECs co-culture group is the highest, suggesting the improved neuronal differentiation of NSCs by CMECs.
Department of Neurosurgery, 3rd Affiliated Hospital, Basic Medical College, Sun Yat-Sen University, Guangzhou, China. gzguoying@hotmail.com
Ying
Y
Guo
Dejin
D
Shi
Wensheng
W
Li
Chaofeng
C
Liang
Hui
H
Wang
Zhuopeng
Z
Ye
Liping
L
Hu
Yan
Y
Li
D000818:Animals; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D042461:Vascular Endothelial Growth Factor A; D020932:Nerve Growth Factor