Alterations in the salivary proteome associated with periodontitis.
OBJECTIVE
To identify changes in the salivary proteome associated with active periodontitis.


METHODS
Quantitative proteomics (two-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis) was used to investigate whole saliva from individuals with severe periodontitis and their proteomic profiles before and after periodontal treatment were compared.


RESULTS
A comparison of 128 proteins across all saliva samples identified 15 protein spots with altered abundance. The predominant alteration observed was an increase in the abundance of the S100 proteins S100A8/A9/A6. Of the remaining proteins with altered abundance, haptoglobin, prolactin inducible protein and parotid secretory protein have previously been associated with host defence.


CONCLUSIONS
These results highlight the predominant involvement of S100 proteins in the host response during periodontitis, identify host defence components that have not been linked previously to this disease and suggest new potential biomarkers for monitoring disease activity in periodontitis.
Haigh
Brendan J
BJ
AgResearch, Hamilton, New Zealand. brendan.haigh@agresearch.co.nz
Stewart
Kevin W
KW
Whelan
John R K
JR
Barnett
Matthew P G
MP
Smolenski
Grant A
GA
Wheeler
Thomas T
TT
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012720:Severity of Illness Index
D020543:Proteome; D009418:S100 Proteins; D012471:Salivary Proteins and Peptides