Genetic modification of the Salmonella membrane physical state alters the pattern of heat shock response.
It is now recognized that membranes are not simple physical barriers but represent a complex and dynamic environment that affects membrane protein structures and their functions. Recent data emphasize the role of membranes in sensing temperature changes, and it has been shown that the physical state of the plasma membrane influences the expression of a variety of genes such as heat shock genes. It has been widely shown that minor alterations in lipid membranes are critically involved in the conversion of signals from the environment to the transcriptional activation of heat shock genes. Previously, we have proposed that the composition, molecular arrangement, and physical state of lipid membranes and their organization have crucial roles in cellular responses during stress caused by physical and chemical factors as well as in pathological states. Here, we show that transformation of Salmonella enterica serovar Typhimurium LT2 (Salmonella Typhimurium) with a heterologous Delta(12)-desaturase (or with its trans-membrane regions) causes major changes in the pathogen's membrane dynamic. In addition, this pathogen is strongly impaired in the synthesis of major stress proteins (heat shock proteins) under heat shock. These data support the hypothesis that the perception of temperature in Salmonella is strictly controlled by membrane order and by a specific membrane lipid/protein ratio that ultimately causes transcriptional activation of heat shock genes. These results represent a previously unrecognized mode of sensing temperature variation used by this pathogen at the onset of infection.
University of Salerno, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Division of BioMedicine, Salerno, Italy.
Amalia
A
Porta
Zsolt
Z
Török
Ibolya
I
Horvath
Silvia
S
Franceschelli
László
L
Vígh
Bruno
B
Maresca
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000502:physiology*; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D018869:Heat-Shock Response; D006358:Hot Temperature; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase