Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles.
Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
Department of Chemistry, University of Massachusetts at Amherst, Amherst, Massachusetts 01003, USA.
Partha
P
Ghosh
Xiaochao
X
Yang
Rochelle
R
Arvizo
Zheng-Jiang
ZJ
Zhu
Sarit S
SS
Agasti
Zhihong
Z
Mo
Vincent M
VM
Rotello
D000818:Animals; D016923:Cell Death; D002460:Cell Line; D002463:Cell Membrane Permeability; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000008:administration & dosage* / Q000378:metabolism
D010455:Peptides; D006046:Gold; D001616:beta-Galactosidase