[In search of the guilty: analysis of encephalitogenic T cell migration pathways on preclinical phase of EAE after their intravenous or intraperitoneal injection].
Adaptive-transfer experimental autoimmune encephalomyelitis is an inflammatory neurodegenerative disease which is induced by injection of activated encephalitogenic T cells. Adaptive-transfer experimental autoimmune encephalomyelitis is a major experimental tool for investigation ofT cell function in multiple sclerosis development. Activated myelin basic protein specific T cells are able to invade and inflame the central nervous system which is followed by axonal injury and paralysis on the third day after injection. In the prodromal phase of EAE encephalitic T cells migrate through different organs which alter their phenotype before invading the CNS. We compared migratory patterns of encephalitic T cells after intravenous and intraperitoneal injection to elucidate which organs may play an important role in the formation of "migratory" phenotype. We found that encephalitogenic T cells ultimately migrate through spleen and parathymic lymph nodes regardless of the start point of cells migration after i.p. and i.v. injection. We hypothesise that cellular and extracellular components of these organs could be involved in the formation of T cells "migratory" phenotype which is necessary for penetration via blood-brain barrier.
M A
MA
Nosov
A
A
Fluegel
E A
EA
Korneva
D019264:Adoptive Transfer; D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D002490:Central Nervous System / Q000276:immunology* / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D017710:Lymphocyte Transfusion; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology