In vitro anti-enterovirus 71 activity of gallic acid from Woodfordia fruticosa flowers.
OBJECTIVE
The anti-enterovirus 71 (EV71) activity of six Nepalese plants' extracts and gallic acid (GA) isolated from Woodfordia fruticosa Kurz (family; Lythaceae) flowers were evaluated in Vero cells.


RESULTS
The anti-EV71 activity of tested compounds was evaluated by a cytopathic effect reduction method. Our results demonstrated that flowers' extracts of W. fruticosa exerted strong anti-EV71 activity, with a 50% inhibitory concentration (IC(50)) of 1.2 microg ml(-1) and no cytotoxicity at a concentration of 100 microg ml(-1), and the derived therapeutic index (TI) was more than 83.33. Rivabirin showed no antiviral activity against EV71. Furthermore, GA isolated from W. fruticosa flowers exhibited a higher anti-EV71 activity than the extract of W. fruticosa flowers, with an IC(50) of 0.76 microg ml(-1) and no cytotoxicity at a concentration of 100 microg ml(-1), and the derived TI was 99.57.


CONCLUSIONS
This study demonstrated that flower extracts of W. fruticosa possessed anti-EV71 activity and GA isolated from these flowers showed stronger anti-EV71 activity than that the extracts.


CONCLUSIONS
Our results suggest that the GA from W. fruticosa flowers may be used as a potential antiviral agent.
Jeonnam Institute of Natural Resources Research, Gisan-ri, Anyang-myeon, Jangheung-gun, Jeollanamdo, South Korea.
H J
HJ
Choi
J H
JH
Song
K S
KS
Park
S H
SH
Baek
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D029821:Enterovirus A, Human / Q000187:drug effects*; D035264:Flowers / Q000737:chemistry; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014709:Vero Cells; D031565:Woodfordia / Q000737:chemistry*
D000998:Antiviral Agents; D010936:Plant Extracts; D005707:Gallic Acid