In vitro study on human trophoblast cells infected with HCMV.
Human trophoblast cells were isolated and cultured in vitro in order to investigate possible pathogenesis of intrauterine infection caused by HCMV. Trophoblast cells were obtained by compound enzymes digestion and discontinuous percoll gradient. Cells and purity were identified by using immunocytochemistry assay with anti-CK7, Vim and beta-hCG antibodies. HCMV AD169 strain replication in isolated trophoblast cells and cell apoptosis were detected at different time points post infection (p.i.). The results showed that highly purified trophoblast cells were obtained. Specific virus replication was increased dramatically at the 24th h p.i., and then increased slowly during 48 h and 72 h. Apoptosis rate of trophoblast cells infected with HCMV was (34.68+/-3.14)% at 24th h p.i., while that in control group was (15.32+/-2.34)% (P<0.05). It was suggested that highly purified trophoblast cells can be isolated by the simplified cell purification method. HCMV can infect human trophoblast cells, and be quickly replicated, resulting in the accelerated apoptosis of human trophoblast cells during early time.
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. cjj8275@163.com
Juan
J
Xiao
Dandan
D
Zhang
Juanjuan
J
Chen
Zongzhi
Z
Yin
Tao
T
Liu
Jihui
J
Ai
Suhua
S
Chen
D017209:Apoptosis / Q000502:physiology*; D002469:Cell Separation; D003587:Cytomegalovirus / Q000472:pathogenicity* / Q000502:physiology; D005260:Female; D006801:Humans; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D061251:Primary Cell Culture; D014327:Trophoblasts / Q000166:cytology / Q000821:virology*; D014779:Virus Replication