KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1.
In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling. [BMB reports 2010; 43(1): 17-22].
Hu
Junjian
J
The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, Peoples' Republic of China.
Yuan
Wuzhou
W
Tang
Ming
M
Wang
Yuequn
Y
Fan
Xiongwei
X
Mo
Xiaoyang
X
Li
Yongqing
Y
Ying
Zaochu
Z
Wan
Yongqi
Y
Ocorr
Karen
K
Bodmer
Rolf
R
Deng
Yun
Y
Wu
Xiushan
X
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D027602:Serum Response Element; D015398:Signal Transduction; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D047908:Intracellular Signaling Peptides and Proteins; C549143:KBTBD7 protein, human; D034741:RNA, Small Interfering; D015534:Trans-Activators; D018808:Transcription Factor AP-1; D020928:Mitogen-Activated Protein Kinases