Insight into cellular response of plant cells confined within silica-based matrices.
The encapsulation of living plant cells into materials could offer the possibility to develop new green biochemical technologies. With the view to designing new functional materials, the physiological activity and cellular response of entrapped cells within different silica-based matrices have been assessed. A fine-tuning of the surface chemistry of the matrix has been achieved by the in situ copolymerization of an aqueous silica precursor and a biocompatible trifunctional silane bearing covalently bound neutral sugars. This method allows a facile control of chemical and physical interactions between the entrapped plant cells and the scaffold. The results show that the cell-matrix interaction has to be carefully controlled in order to avoid the mineralization of the cell wall which typically reduces the bioavailability of nutrients. Under appropriate conditions, the introduction of a trifunctional silane (ca. 10%) during the preparation of hybrid gels has shown to prolong the biological activity as well as the cellular viability of plant cells. The relations of cell behavior with some other key factors such as the porosity and the contraction of the matrix are also discussed.
Meunier
Christophe F
CF
Laboratory of Inorganic Materials Chemistry (CMI), The University of Namur (FUNDP), 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Rooke
Joanna C
JC
Hajdu
Kata
K
Van Cutsem
Pierre
P
Cambier
Pierre
P
Léonard
Alexandre
A
Su
Bao-Lian
BL
D000327:Adsorption; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009584:Nitrogen / Q000737:chemistry; D059828:Plant Cells; D010944:Plants / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*
D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D009584:Nitrogen