Comparison of the chromosome maps around a resistance hot spot on chromosome 5 of potato and tomato using BAC-FISH painting.
Potato chromosome 5 harbours numerous genes for important qualitative and quantitative traits, such as resistance to the root cyst nematode Globodera pallida and the late blight fungus, Phytophthora infestans. The genes make up part of a "hot spot" for resistances to various pathogens covering a genetic map length of 3 cM between markers GP21 and GP179. We established the physical size and position of this region on chromosome 5 in potato and tomato using fluorescence in situ hybridization (FISH) on pachytene chromosomes. Five potato bacterial artificial chromosome (BAC) clones with the genetically anchored markers GP21, R1-contig (proximal end), CosA, GP179, and StPto were selected, labeled with different fluorophores, and hybridized in a five-colour FISH experiment. Our results showed the location of the BAC clones in the middle of the long arm of chromosome 5 in both potato and tomato. Based on chromosome measurements, we estimate the physical size of the GP21-GP179 interval at 0.85 Mb and 1.2 Mb in potato and tomato, respectively. The GP21-GP179 interval is part of a genome segment known to have inverted map positions between potato and tomato.
Achenbach
Ute C
UC
Max Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, Köln, Germany.
Tang
Xiaomin
X
Ballvora
Agim
A
de Jong
Hans
H
Gebhardt
Christiane
C
D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D005456:Fluorescent Dyes / Q000378:metabolism; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics*; D020161:Physical Chromosome Mapping; D040641:Quantitative Trait Loci; D011198:Solanum tuberosum / Q000235:genetics*
D005456:Fluorescent Dyes; D005819:Genetic Markers; D007211:Indoles; C007293:DAPI