Illuminating the ubiquitin/proteasome system.
The ubiquitin/proteasome system (UPS) is responsible for the regulated processive degradation of proteins residing in the cytosol, nucleus, and endoplasmic reticulum. The two central players are ubiquitin, a small protein that is conjugated to substrates, and the proteasome, a large multi-subunit proteolytic complex that executes degradation of ubiquitylated proteins. Ubiquitylation and proteasomal degradation are highly dynamic processes. During the last decade, many researchers have started taking advantage of fluorescent proteins, which allow studying the dynamic nature of this system in the context of its natural environment: the living cell. In this review, we will summarize studies that have implemented this approach to examine the UPS and discuss novel insights in the dynamic organization of the UPS.
Department of Cell and Molecular Biology, Karolinska Institutet, S-17177 Stockholm, Sweden.
Florian A
FA
Salomons
Klàra
K
Acs
Nico P
NP
Dantuma
D000818:Animals; D005453:Fluorescence; D049452:Green Fluorescent Proteins; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000502:physiology*
D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex