[Generation of factor VIII gene knockout mouse by tetraploid embryo complementation technology].
OBJECTIVE
Factor VIII( FVIII) gene knockout mouse model was established for further study on the treatment of hemophilia A.


METHODS
Exons 16-19 of the mouse FVIII gene were knocked out by ET clone, ES homologous recombination and tetraploid embryo compensation technology. PCR, reverse transcriptase-PCR(RT-PCR) and immunohistochemistry were used to detect the transcription and translation pattern of FVIII. The phenotype of the knockout mice was analyzed by examining the activated partial thromboplastin time (APTT) and FVIII activity (FVIII:C).


RESULTS
PCR, RT-PCR and immunohistochemistry confirmed that FVIII was deficient in the FVIII gene knockout mouse. The APTT results showed that FVIII-deficient mouse plasma had a prolonged clotting time compared to normal mouse plasma. The FVIII:C in heterozygous, hemizygous and homozygous mice was 80%, 8% and 10% of that in normal mice, respectively.


CONCLUSIONS
The phenotype of the FVIII gene knockout mouse appears grossly similar to that of human with hemophilia A. Establishment of this model may promote the development of new technologies of treatment to hemophilia A.
Kuang
Ying
Y
Shanghai Research Center for Biomodel Organism, Shanghai, 201210 P.R.China.
Wang
Jinjin
J
Lu
Xibin
X
Lu
Shunyuan
S
Zhang
Liangliang
L
Shen
Chunling
C
Fei
Jian
J
Wang
Zhugang
Z
D000818:Animals; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D005169:Factor VIII / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006467:Hemophilia A / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D010314:Partial Thromboplastin Time
D005169:Factor VIII