Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis.
Bovine tuberculosis caused by Mycobacterium bovis is an important zoonosis. In this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. The technique will be of value in the clinical and field-based diagnosis of M. bovis and can differentiate it from other bacteria such as Corynebacterium diphtheriae, Streptococcus pneumoniae, β-haemolytic streptococci, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Staphylococcus aureus, and Klebsiella pneumoniae. The limit of detection was 10 copies/μL and the results were corroborated by PCR. The method was highly specific and more sensitive than PCR.
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, People's Republic of China.
Jun
J
Zhang
Gui-Hong
GH
Zhang
Lin
L
Yang
Ren
R
Huang
Yu
Y
Zhang
Kun
K
Jia
Wen
W
Yuan
Shou-Jun
SJ
Li
D000818:Animals; D002417:Cattle; D005784:Gene Amplification; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000592:standards / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000175:diagnosis*