Identification and characterization of a mosquito pupicidal metabolite of a Bacillus subtilis subsp. subtilis strain.
The culture supernatant of a strain of Bacillus subtilis subsp. subtilis isolated from mangrove forests of Andaman and Nicobar islands, India was found to kill larval and pupal stages of mosquitoes. A chloroform extract of the culture supernatant of the bacterium showed pupicidal effects at an LC(50) dose of 1 microg/ml. The mosquitocidal metabolite(s) produced by this strain were purified by gel permeation chromatography. The purified fraction was subjected to Fourier transform infrared (FTIR) spectroscopy and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The FTIR spectrum of active fraction/CHCl3 residue showed strong band characteristic of peptides. MALDI-TOF spectrum of the sample showed well-resolved group of peaks at m/z values 1,030.6, 1,046.7, 1,044.6, 1,060.5, 1,058.6, 1,058.7, and 1,074.6. The results indicated production of different isoforms of surfactin, ranging from C13-C15. Further, the sfp gene responsible for the production of surfactin was amplified and sequenced. In conclusion, this study showed that the mosquito pupicidal metabolite(s), produced by B. subtilis subsp. subtilis is the cyclic lipopeptide, surfactin. The mode of action of surfactin on pupae of mosquitoes is discussed. This is the first report on the mosquito pupicidal activity of surfactin produced by B. subtilis subsp. subtilis.
Unit of Microbiology and Biotechnology, Vector Control Research Centre, Indian Council of Medical Research, Indira Nagar, Puducherry, 605 006, India. iglory@yahoo.com
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Geetha
A M
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Manonmani
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KP
Paily
D000818:Animals; D000852:Anopheles / Q000254:growth & development; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D005798:Genes, Bacterial; D007194:India; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011679:Pupa / Q000254:growth & development; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D007306:Insecticides; D055666:Lipopeptides; D010456:Peptides, Cyclic; C082662:phosphopantetheinyl transferase; C009365:surfactin peptide; D017855:Transferases (Other Substituted Phosphate Groups); D010447:Peptide Hydrolases