A mutation in H5 haemagglutinin that conferred human receptor recognition is not maintained stably during duck passage.
A/Hong Kong/213/97 (HK213; H5N1), isolated from a human, binds to both avian- and human-type receptors, due to a haemagglutinin (HA) mutation probably acquired during adaptation to humans. Duck passage of this virus conferred lethality in ducks. Sequence analyses of the duck-passaged virus revealed that its HA gene reverted back to one recognizing only avian-type receptors, and consequently it bound human tissue to a lesser extent. This finding suggests that viruses with human-type receptor specificity are unlikely to be maintained in waterfowl, unlike those with the human-type PB2 mutation, such as H5N1 viruses of the Qinghai Lake lineage.
Division of Zoonosis, Department of Microbiology and Infectious Diseases, Graduate School of Medicine, Kobe University 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, Japan. kyoko.tanaka@aioros.ocn.ne.jp
Kyoko
K
Shinya
Akiko
A
Makino
Masato
M
Hatta
Shinji
S
Watanabe
Jin Hyun
JH
Kim
Yoshihiro
Y
Kawaoka
D000818:Animals; D004372:Ducks; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D011201:Poultry Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D016019:Survival Analysis; D053585:Virus Attachment
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral; C109963:hemagglutinin, avian influenza A virus