Chemical inducers of targeted protein degradation.
The functions of many cellular proteins have been elucidated by selective gene inactivation and subsequent phenotypic analysis. For example, genetic mutations, gene knock-out generation, and the use of RNA interference to target mRNA for degradation can all result in decreased production of a specific protein, yielding informative cellular phenotypes. However, these techniques each have certain inherent limitations. This minireview focuses on the recent development of new approaches to study protein function at the post-translational level, namely chemical induction of targeted protein degradation.
Raina
Kanak
K
Department of Chemistry, Yale University, New Haven, Connecticut 06520-8103, USA.
Crews
Craig M
CM
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D010447:Peptide Hydrolases / Q000737:chemistry; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D025801:Ubiquitin / Q000737:chemistry
D011506:Proteins; D012333:RNA, Messenger; D025801:Ubiquitin; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex