A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia.
A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.
Laboratory of Virology, Department of Plant Sciences, Wageningen University, Wageningen, The Netherlands.
Afshin
A
Hassani-Mehraban
Marleen
M
Botermans
J Th J
JT
Verhoeven
Ellis
E
Meekes
Janneke
J
Saaijer
Dick
D
Peters
Rob
R
Goldbach
Richard
R
Kormelink
D031402:Alstroemeria / Q000821:virology*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003105:Colombia; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D017865:Tospovirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins