Iodine scanning of a phenazine inhibitor of vacuolar sorting.
Affinity reagents are often used to address the target identification problem in chemical genetics. The design of such reagents so that the linker does not occlude interactions with protein targets is an ongoing challenge. This work describes a systematic approach to synthesize derivatives of a bioactive that should avoid interference with binding to targets and be readily converted to affinity reagents.
Department of Botany and Plant Sciences and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
Marci
M
Surpin
Yunfan
Y
Zou
Chiyi
C
Xiong
Natasha V
NV
Raikhel
Michael C
MC
Pirrung
D017360:Arabidopsis / Q000378:metabolism; D007455:Iodine / Q000737:chemistry*; D010619:Phenazines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship; D014617:Vacuoles / Q000378:metabolism*
D010619:Phenazines; D011506:Proteins; D007455:Iodine