Isolation of genomic DNA from Ilyanassa snail larvae.
The marine gastropod Ilyanassa obsoleta is a long-standing and very useful model for studies of embryonic development. It is an especially important model for spiralian development, and for studies of asymmetric cell division. The embryos are amenable to classic embryological manipulation techniques as well as a growing number of molecular approaches. Ilyanassa is also an important model for studies of metamorphosis, the ecology of parasitism, the effects of environmental contaminants on morphology and sexual function, and comparative neurobiology. Ilyanassa is host to several species of parasitic trematode worms, so care must be taken to avoid contamination of Ilyanassa genomic DNA with that of the parasites. The easiest way to avoid this contamination is to isolate DNA from veliger larvae, which are not parasitized. This also avoids other problems that can be encountered when isolating DNA from adult mollusc tissues, such as the presence of large amounts of polysaccharides. This protocol describes the isolation of genomic DNA from Ilyanassa larvae.
Gharbiah
Maey
M
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA.
Cooley
James
J
Leise
Esther M
EM
Nakamoto
Ayaki
A
Rabinowitz
Jeremy S
JS
Lambert
J David
JD
Nagy
Lisa M
LM
D000818:Animals; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D005821:Genetic Techniques; D016678:Genome; D007814:Larva / Q000235:genetics* / Q000254:growth & development; D023421:Models, Animal; D012908:Snails / Q000196:embryology* / Q000235:genetics*
D004247:DNA