Production of mouse chimeras by injection of embryonic stem cells into the perivitelline space of one-cell stage embryos.
Generation of mouse chimeras is useful for the elucidation of gene function. In the present report, we describe a new technique for the production of chimeras by injection of R1 embryonic stem (ES) cells into the perivitelline space of one-cell stage mouse embryos. One-cell embryos are injected with 2-6 ES cells into the perivitelline space under the zona pellucida without laser-assistance. Our embryo culture experiments reveal that ES cells injected at the one-cell stage embryo start to be incorporated into the blastomeres beginning at the 8-cell stage and form a chimeric blastocyst after 4 days. We have used this approach to successfully produce a high rate of mouse chimeras in two different mouse genetic backgrounds permitting the establishment of germ line transmitters. This method allows for the earlier introduction of ES cells into mouse embryos, and should free up the possibility of using frozen one-cell embryos for this purpose.
Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada.
Yves
Y
De Repentigny
Rashmi
R
Kothary
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D017690:Cell Transplantation / Q000379:methods; D002970:Cleavage Stage, Ovum / Q000166:cytology* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000637:transplantation*; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D018183:Transplantation Chimera / Q000235:genetics
D008164:Luminescent Proteins; D011994:Recombinant Proteins