Genetic polymorphisms in metabolizing enzymes and susceptibility of chromosomal damage induced by vinyl chloride monomer in a Chinese worker population.
OBJECTIVE
To evaluate whether polymorphisms in metabolizing enzymes contributed to susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM).


METHODS
Cytokinesis block micronucleus test was performed on 185 VCM-exposed workers and 41 control subjects to detect chromosomal damage in peripheral lymphocytes. The polymerase chain reaction and restriction fragment length polymorphism technique was applied to detect polymorphisms of GSTT1, GSTM1, GSTP1G/A, CYP2E1G/C, and CYP2D6G/C. Poisson regression analysis was performed.


RESULTS
Sex, age, VCM exposure, GSTP1, and CYP2E1 genotype can influence chromosomal damage. There was a 1.51-fold increased micronucleus frequency for GSTP1GG genotypes individuals compared with those GSTP1AA/GA genotype individuals (P < 0.05), the effect of polymorphism in CYP2E1 gene was more pronounced for allele C compared with allele G (P < 0.05).


CONCLUSIONS
Polymorphisms of GSTP1G/A and CYP2E1G/C, which are potential susceptibility biomarkers of chromosomal damage in VCM-exposed worker.
Department of Occupational Health and Toxicology, School of Public Health, Fudan University, China.
Wei
W
Wang
Yu-lan
YL
Qiu
Fang
F
Ji
Jing
J
Liu
Fen
F
Wu
Wen-bin
WB
Miao
Yongliang
Y
Li
Paul W
PW
Brandt-Rauf
Zhao-lin
ZL
Xia
D000328:Adult; D000367:Age Factors; D002617:Chemical Industry; D016009:Chi-Square Distribution; D002681:China; D002869:Chromosome Aberrations / Q000139:chemically induced*; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D004781:Environmental Exposure / Q000009:adverse effects; D004798:Enzymes / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000009:adverse effects*; D011110:Polymorphism, Genetic / Q000187:drug effects* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012044:Regression Analysis; D012737:Sex Factors; D014752:Vinyl Chloride / Q000633:toxicity*; D000078329:Workforce
D019389:Cytochrome P-450 CYP2D6; D019392:Cytochrome P-450 CYP2E1; D004798:Enzymes; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D014752:Vinyl Chloride; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human