Complete HOX cluster characterization of the coelacanth provides further evidence for slow evolution of its genome.
The living coelacanth is a lobe-finned fish that represents an early evolutionary departure from the lineage that led to land vertebrates, and is of extreme interest scientifically. It has changed very little in appearance from fossilized coelacanths of the Cretaceous (150 to 65 million years ago), and is often referred to as a "living fossil." An important general question is whether long-term stasis in morphological evolution is associated with stasis in genome evolution. To this end we have used targeted genome sequencing for acquiring 1,612,752 bp of high quality finished sequence encompassing the four HOX clusters of the Indonesian coelacanth Latimeria menadoensis. Detailed analyses were carried out on genomic structure, gene and repeat contents, conserved noncoding regions, and relative rates of sequence evolution in both coding and noncoding tracts. Our results demonstrate conclusively that the coelacanth HOX clusters are evolving comparatively slowly and that this taxon should serve as a viable outgroup for interpretation of the genomes of tetrapod species.
Benaroya Research Institute, Virginia Mason, Seattle, WA 98101, USA.
Chris T
CT
Amemiya
Thomas P
TP
Powers
Sonja J
SJ
Prohaska
Jane
J
Grimwood
Jeremy
J
Schmutz
Mark
M
Dickson
Tsutomu
T
Miyake
Michael A
MA
Schoenborn
Richard M
RM
Myers
Francis H
FH
Ruddle
Peter F
PF
Stadler
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D023061:Gene Order; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family
D018398:Homeodomain Proteins