Dynamics of sperm DNA fragmentation in the swine: ejaculate and temperature effects.
The dynamics of sperm DNA fragmentation was examined in 16 boar ejaculates using the sperm chromatin dispersion (SCD) test and a two-tail comet assay. The net sperm rich fraction was preserved at two different temperatures (Trial 1: 15 degrees C, n=10; Trial 2: 37 degrees C, n=6) and sub-samples were taken every day until a sperm motility of zero. Significant differences in the dynamics of DNA fragmentation were observed among the different ejaculates and also according to the storage temperature. After analyzing the dynamic response of the sperm DNA damage, when the sperm samples are incubated at 15 or 37 degrees C, each ejaculate could be classified and a considerable variation among individuals for an increase in DNA damage was observed. Thus, while in some ejaculates no rise in DNA fragmentation was observed, in others, the sperm DNA fragmentation process was triggered during the initial days of the experiment. In general, sperm incubation at 37 degrees C diminished sperm DNA quality. The two-tail comet assay indicated that at time zero existing DNA damage mainly consisted of double stranded DNA breakage. During storage, DNA damage affected one of the DNA strands until a second wave of DNA damage, in which there was both single and double stranded DNA damage.
Gestión Veterinaria Porcina S.L., C/Calibre 121, C. Villalba, Madrid, Spain. bperez@acromax.net
B
B
Pérez-Llano
C
C
López-Fernández
P
P
García-Casado
F
F
Arroyo
A
A
Gosalbez
R
R
Sala
J
J
Gosálvez
D000818:Animals; D002843:Chromatin / Q000648:ultrastructure; D020552:Comet Assay; D053938:DNA Fragmentation; D004542:Ejaculation; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D013552:Swine; D013696:Temperature; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary
D002843:Chromatin