Agrobacterium rhizogenes-mediated transformation of Hypericum tomentosum L and Hypericum tetrapterum Fries.
This is the first evidence on successful Agrobacterium rhizogenes-mediated genetic transformation of two species from the genus Hypericum, H. tomentosum L. and H. tetrapterum Fries. Hairy root cultures were induced from root segments of both Hypericum species by two agropine wild-type strains of A. rhizogenes, ATCC 15834 and A4. The transgenic character of the hairy root cultures was proved by PCR amplification of the rolABCD genes. In some H. tetrapterum transgenic lines aux genes were detected as well.
P. J. Safirik University, Faculty of Science, Institute of Biology and Ecology, Mánesova 23, 041 54 KoSice, Slovakia.
Hedviga
H
Komarovská
Annalisa
A
Giovannini
Ján
J
Kosutha
Eva
E
Cellárovia
D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D020902:Hypericum / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D004269:DNA, Bacterial