Characterization of the Aspergillus nidulans monodictyphenone gene cluster.
Deletion of cclA, a component of the COMPASS complex of Aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. Through a set of targeted deletions in a cclA deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. Identification of an intermediate, endocrocin, from an mdpHDelta strain suggests that mdpH might encode a decarboxylase. Furthermore, by replacing the promoter of mdpA (a putative aflJ homolog) and mdpE (a putative aflR homolog) with the inducible alcA promoter, we have confirmed that MdpA functions as a coactivator. We propose a biosynthetic pathway for monodictyphenone and emodin derivatives based on bioinformatic analysis and characterization of biosynthetic intermediates.
Graduate Institute of Pharmaceutical Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan, Republic of China.
Yi-Ming
YM
Chiang
Edyta
E
Szewczyk
Ashley D
AD
Davidson
Ruth
R
Entwistle
Nancy P
NP
Keller
Clay C C
CC
Wang
Berl R
BR
Oakley
D000880:Anthraquinones / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D017353:Gene Deletion; D005800:Genes, Fungal; D005810:Multigene Family; D013057:Spectrum Analysis
D000880:Anthraquinones