Larkinella bovis sp. nov., isolated from fermented bovine products, and emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006.
A novel bacterial strain, designated M2T2B15(T), was isolated from fermented bovine products and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Cells were strictly aerobic, Gram-reaction-negative, oxidase- and catalase-positive rods that lacked flagella and were motile by gliding. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that strain M2T2B15(T) was related most closely to Larkinella insperata LMG 22510(T) (94.4 % similarity) but shared <87 % similarity with other members of the phylum Bacteroidetes. The major cellular fatty acids were C(16 : 1)ω5c, iso-C(15 : 0) and iso-C(17 : 0) 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylserine, two unidentified aminophospholipids and two unidentified polar lipids. Menaquinone 7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain M2T2B15(T) was 52&emsp14;mol%. The phenotypic, genotypic and phylogenetic data presented clearly indicate that strain M2T2B15(T) represents a novel species of the genus Larkinella, for which the name Larkinella bovis sp. nov. is proposed. The type strain is M2T2B15(T) (=KACC 14040(T) =NBRC 106324(T)). Emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006 are also proposed.
Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625 104, Tamil Nadu, India.|Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Rangasamy
R
Anandham
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Soon-Wo
SW
Kwon
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Hang-Yeon
HY
Weon
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Soo-Jin
SJ
Kim
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Yi-Seul
YS
Kim
Regional Research Station, Tamil Nadu Agricultural University, Vriddhachalam 606001, Tamil Nadu, India.
Pandiyan Indira
PI
Gandhi
Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Yong Ki
YK
Kim
Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Hyeong Jin
HJ
Jee
D000818:Animals; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D016000:Cluster Analysis; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D008124:Locomotion; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011090:Polyenes / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011090:Polyenes; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C017313:flexirubins; D010088:Oxidoreductases; D002374:Catalase