Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells.
The umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities.
Cai
Jinglei
J
Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China.
Li
Wen
W
Su
Huanxing
H
Qin
Dajiang
D
Yang
Jiayin
J
Zhu
Fan
F
Xu
Jianyong
J
He
Wenzhi
W
Guo
Xiangpeng
X
Labuda
Krystyna
K
Peterbauer
Anja
A
Wolbank
Susanne
S
Zhong
Mei
M
Li
Zhiyuan
Z
Wu
Wutian
W
So
Kwok-Fai
KF
Redl
Heinz
H
Zeng
Lingwen
L
Esteban
Miguel Angel
MA
Pei
Duanqing
D
D000650:Amnion / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009046:Motor Neurons / Q000378:metabolism; D018408:Patch-Clamp Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism*