Synthesis of a trigalacturonic acid analogue mimicking the expected transition state in the glycosidases.
A trigalacturonic acid analogue carrying a cyclohexene framework in place of the central pyranose ring was synthesized as a molecular probe for the mechanistic investigation of endo-polygalacturonase 1 (endo-PG 1). Preliminary enzymatic studies revealed that this analogue inhibited endo-PG 1 activity by about 30% at 0.3mM concentration.
Faculty of Agriculture and Life Science, Hirosaki University, 3-Bunkyo-cho, Hirosaki 036-8561, Japan.
Kazunori
K
Yamamoto
Shogo
S
Noguchi
Noboru
N
Takada
Kazuo
K
Miyairi
Masaru
M
Hashimoto
D006603:Hexuronic Acids / Q000737:chemistry*; D008958:Models, Molecular; D018716:Molecular Mimicry; D011096:Polygalacturonase / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
D006603:Hexuronic Acids; C007819:galacturonic acid; D011096:Polygalacturonase