ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.
We have previously performed an unbiased screen to identify genes whose expression is associated with the metastatic phenotype. Secondary screening of these genes using custom microarray chips identified ASAP1, a multi-domain adaptor protein with ADP-ribosylation factor-GAP activity, as being potentially involved in tumor progression. Here, we show that at least three different splice forms of ASAP1 are upregulated in rodent tumor models in a manner that correlates with metastatic potential. In human cancers, we found that ASAP1 expression is strongly upregulated in a variety of tumors in comparison with normal tissue and that this expression correlates with poor metastasis-free survival and prognosis in colorectal cancer patients. Using loss and gain of function approaches, we were able to show that ASAP1 promotes metastasis formation in vivo and stimulates tumor cell motility, invasiveness, and adhesiveness in vitro. Furthermore, we show that ASAP1 interacts with the metastasis-promoting protein h-prune and stimulates its phosphodiesterase activity. In addition, ASAP1 binds to the SH3 domains of several proteins, including SLK with which it co-immunoprecipitates. These data support the notion that ASAP1 can contribute to the dissemination of a variety of tumor types and represent a potential target for cancer therapy.
Forschungszentrum Karlsruhe, Institute of Genetics, Karlsruhe, Germany.
T
T
Müller
U
U
Stein
A
A
Poletti
L
L
Garzia
M
M
Rothley
D
D
Plaumann
W
W
Thiele
M
M
Bauer
A
A
Galasso
P
P
Schlag
M
M
Pankratz
M
M
Zollo
J P
JP
Sleeman
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology*; D017398:Alternative Splicing; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D020869:Gene Expression Profiling; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051381:Rats; D018909:src Homology Domains
C117891:ASAP1 protein, human; D048868:Adaptor Proteins, Signal Transducing