Antisecretory, antioxidative and antiapoptotic effects of montelukast on pyloric ligation and water immersion stress induced peptic ulcer in rat.
In the present study, we tried to explore the mechanism of montelukast as an antiulcerogenic agent in pyloric ligation (PL) and water immersion stress (WIS) induced peptic ulcer. The ameliorative effects of montelukast (5, 10, and 20 mg/kg, p.o.) on gastric volume and total acidity were studied in PL model. We have investigated the alteration in the ulcerative index, thiobarbituric acid reactive substances, reduced glutathione, activity of myeloperoxidase, and total calcium level in both models. Estimation of DNA fragmentation by gel electrophoresis was also performed. Medium and higher doses of montelukast showed significant (p<0.05) ameliorative potential on all the above parameters as compared with omeprazole treated group. DNA fragmentation pattern clearly indicated the antiapoptotic effect of montelukast in preventing mucosal erosion in both models. Hence, the gastroprotective effect of montelukast may be attributed to its antisecretory, antioxidative along with its antiapoptotic effect.
Muthuraman
Arunachalam
A
Rayat Institute of Pharmacy, Nawanshahr District, Railmajra, Near Ropar, Punjab 144533, India. aruachalammu@gmail.com
Sood
Shailja
S
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D003521:Cyclopropanes; D053938:DNA Fragmentation / Q000187:drug effects; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D020024:Leukotriene Antagonists / Q000494:pharmacology*; D008297:Male; D010437:Peptic Ulcer / Q000097:blood* / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D009195:Peroxidase / Q000097:blood; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000473:pathology; D013315:Stress, Psychological / Q000097:blood* / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D013440:Sulfides; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000085:Acetates; D003521:Cyclopropanes; D020024:Leukotriene Antagonists; D011804:Quinolines; D013440:Sulfides; D017392:Thiobarbituric Acid Reactive Substances; D009195:Peroxidase; D005978:Glutathione; C093875:montelukast