PCR-based detection of Leishmania major kDNA within naturally infected Phlebotomus papatasi in southern Iran.
The annual incidence of cutaneous leishmaniasis (CL) in Iran rose by 43% over a five year period, from 2002 to 2006; most of these cases were caused by Leishmania major. Two complementary standard and nested polymerase chain reactions (PCRs) were used to detect parasites within their natural vector, Phlebotomus papatasi. Twelve different sand fly species were morphologically identified. The most abundant species (31.3%) was P. papatasi. Leptomonads were found in nine (2.4%) phlebotomines. Twenty (5.3%) sand fly species were found positive for Leishmania-genus DNA using standard PCR. The infection rate of this species was 5% and 7% by microscopic and molecular methods, respectively.
Azizi
Kourosh
K
Department of Medical Entomology, Research Centre for Health Sciences, School of Health and Nutrition, Shiraz University of Medical Sciences, P.O. Box 71645-111, Shiraz, Iran. azizik@hums.ac.ir
Rassi
Yavar
Y
Moemenbellah-Fard
Mohammad Djaefar
MD
D000818:Animals; D018105:DNA, Kinetoplast / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D004197:Disease Reservoirs; D005260:Female; D006801:Humans; D007303:Insect Vectors; D007492:Iran; D018320:Leishmania major / Q000145:classification* / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000635:transmission*; D008297:Male; D010691:Phlebotomus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction
D018105:DNA, Kinetoplast; D016054:DNA, Protozoan