Errors and reproducibility of DNA array-based detection of allelic variants in ADME genes: PHARMAchip.
OBJECTIVE
Differences in adverse drug reactions can be explained by genetic variations, especially if they determine the expression of certain protein effectors and/or drug-metabolizing enzymes. Over the last decade, several tests screening for the most frequent polymorphisms in drug-metabolizing enzymes have been marketed for research and diagnostic purposes. The aim of this study was to assess the suitability of PHARMAchip for the genotyping of polymorphisms of genes associated with drug metabolism and response as an alternative to Jurilab Ltd's DrugMEt Test.


METHODS
In this observational study, performed using 100 previously genotyped DNA samples, we report on common genes included in the two different tests examined: the former DrugMEt test and the recently introduced PHARMAchip test.


CONCLUSIONS
Although these tests are based on different methodological approaches, we have found a high concordance of results between both methods. Some of the discrepancies between tests were related to allelic variants not monitored in a particular microarray and the quality of the genomic DNA used.
Human Pharmacology & Clinical Neurosciences Research Group of the Neuropsychopharmacology Program, Institut Municipal d'Investigació Mèdica (IMIM-Hospital del Mar), Parc de Recerca Biomèdica de Barcelona, c/ Dr. Aiguader 88/08003 Barcelona, Spain. rtorre@imim.es
Elisabet
E
Cuyàs
Estibaliz
E
Olano-Martín
Olha
O
Khymenets
Lorena
L
Hernández
Laia
L
Jofre-Monseny
Laura
L
Grandoso
Diego
D
Tejedor
Antonio
A
Martínez
Magí
M
Farré
Rafael
R
de la Torre
D016477:Artifacts; D003580:Cytochromes / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004358:Drug Therapy; D064420:Drug-Related Side Effects and Adverse Reactions; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010597:Pharmacogenetics / Q000592:standards*; D011110:Polymorphism, Genetic / Q000235:genetics; D015203:Reproducibility of Results
D003580:Cytochromes; D004364:Pharmaceutical Preparations; D004247:DNA