[Study on the automatic ribotyping for Enterobacter sakazakii].
OBJECTIVE
To analyze the ribotyping fingerprint of Enterobacter sakazakii (E. sakazakii) isolated from food and its typing power.


METHODS
Two standard strains and twenty-eight isolates of E.sakazakii were analyzed by the DuPont Riboprinter(TM) microbial characterization system. The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software.


RESULTS
This system grouped two standard strains and twenty-eight E.sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively, the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters.


CONCLUSIONS
The automatic ribotyping method is convenient and fast in E.sakazakii typing.
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
Xiao-yan
XY
Pei
Yun-chang
YC
Guo
Xiu-mei
XM
Liu
D044083:Cronobacter sakazakii / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005516:Food Microbiology; D021521:Ribotyping / Q000379:methods*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal