Thrombin receptors in vascular smooth muscle cells - function and regulation by vasodilatory prostaglandins.
The vast majority of thrombin (>95%) is generated after clotting is completed, suggesting that thrombin formation serves purposes beyond coagulation, such as tissue repair after vessel injury. Two types of vascular thrombin binding sites exist: protease-activated receptors (PARs) and thrombomodulin (TM). Their expression is low in contractile vascular smooth muscle cells (SMC), the dominating subendothelial cell population, but becomes markedly up-regulated upon injury. In human SMC, PAR-1, PAR-3, and PAR-4 mediate thrombin-induced proliferation, migration and matrix biosynthesis as well as generation of inflammatory and growth-promoting mediators. Thrombin-responsive PARs are transcriptionally down-regulated in human vascular SMC by vasodilatory prostaglandins (PGI2/PGE2). For PAR-1 and PAR-3 this mechanism involves cAMP-dependent inactivation of the transcription factor NFAT. The human PAR-4 promoter does not possess NFAT recognition motifs suggesting involvement of other cAMP-regulated effectors. Unlike PARs, TM is induced in SMC exposed to vasodilatory prostaglandins. Enhanced thrombin binding to TM might ameliorate PAR-mediated SMC stimulation. Also expressed in human SMC is the endothelial protein C receptor (EPCR), which serves as an anchor to facilitate generation of activated protein C (aPC) by TM-bound thrombin. Whether prostaglandins affect aPC-generation is not known. In SMC, thrombin and aPC act synergistically via PAR-1 to modify tissue remodelling, in contrast to their antagonistic interaction in the coagulation pathways. Overall, this will contribute to plaque stability and wound healing. The processes outlined here are likely to become clinically relevant after up-regulation of vascular cyclooxygenase2, the rate limiting step in vascular PGE2/PGI2 biosynthesis, such as in advanced atherosclerosis and acute coronary syndromes.
Schrör
Karsten
K
Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Universitätsstrasse 1, Gebäude 22.21, 40225 Düsseldorf, Germany. karsten.schroer@uni-duesseldorf.de
Bretschneider
Ellen
E
Fischer
Kerstin
K
Fischer
Jens W
JW
Pape
Robert
R
Rauch
Bernhard H
BH
Rosenkranz
Anke C
AC
Weber
Artur-Aron
AA
D054058:Acute Coronary Syndrome / Q000378:metabolism / Q000473:pathology; D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology; D001777:Blood Coagulation; D005786:Gene Expression Regulation; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D011453:Prostaglandins / Q000378:metabolism*; D018179:Receptors, Thrombin / Q000235:genetics / Q000378:metabolism*; D013927:Thrombosis / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D014664:Vasodilation; D014945:Wound Healing
D011453:Prostaglandins; D018179:Receptors, Thrombin