Oxidative stress status and lipid profiles of diabetic pregnant rats exposed to cigarette smoke.
This study aimed to evaluate the oxidative stress status and the concentrations of triglycerides, cholesterol and total proteins of pregnant rats exposed to the association of diabetes and cigarette smoke. Female Wistar rats were randomly distributed in four experimental groups, according to presence or not of diabetes and the exposure or not to cigarette smoke. Diabetes was induced by streptozotocin (40 mg/kg i.v.) and exposure to cigarette smoke was for 30 min, twice a day, for 2 months. At day 21 of pregnancy, blood was collected for total protein, triglyceride, cholesterol and oxidative stress determinations. Data were analysed by ANOVA followed by Student-Newman-Keuls test (P<0.05). The association of diabetes and exposure to cigarette smoke was related to the incidence of hypertriglyceridaemia, and this result was due to the severe diabetes and not to exposure to smoke. There was no alteration to protein metabolism in pregnant rats. Diabetes and cigarette smoke exposure led to the activation of the antioxidant system in an attempt to detoxify the organism in face of high lipid peroxidation, which can be characterized by the determination of reactive substances to thiobarbituric acid.
Laboratory of Experimental Research of Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Botucatu Medical School, UNESP/Univ Estadual Paulista, São Paulo State, Brazil.
Maricelma da Silva Soares
Mda S
de Souza
Yuri Karen
YK
Sinzato
Paula Helena Ortiz
PH
Lima
Iracema Mattos Paranhos
IM
Calderon
Marilza Vieira Cunha
MV
Rudge
Débora Cristina
DC
Damasceno
D000818:Animals; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D018384:Oxidative Stress; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012906:Smoke; D012907:Smoking; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014028:Tobacco Smoke Pollution; D014280:Triglycerides / Q000097:blood
D008055:Lipids; D012906:Smoke; D017392:Thiobarbituric Acid Reactive Substances; D014028:Tobacco Smoke Pollution; D014280:Triglycerides; D002784:Cholesterol