Heterogeneity in progenitor cell subtypes in the ventricular zone of the zebrafish adult telencephalon.
The zebrafish has become a new model for adult neurogenesis, owing to its abundant neurogenic areas in most brain subdivisions. Radial glia-like cells, actively proliferating cells, and label-retaining progenitors have been described in these areas. In the telencephalon, this complexity is enhanced by an organization of the ventricular zone (VZ) in fast and slow-dividing domains, suggesting the existence of heterogeneous progenitor types. In this work, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of the adult zebrafish telencephalon (identified by expression of proliferating cell nuclear antigen (PCNA), MCM5, or bromodeoxyuridine incorporation). We demonstrate the existence of distinct populations of dividing cells at the adult telencephalic VZ. Progenitors of the overall slow-cycling domains express high levels of Sox2 and nestin:gfp as well as all glial markers tested. In contrast, domains with an overall fast division rate are characterized by low or missing expression of glial markers. PCNA-positive cells in fast domains further display a morphology distinct from radial glia and co-express PSA-NCAM, suggesting that they are early neuronal precursors. In addition, the VZ contains cycling progenitors that express neither glial markers nor nestin:gfp, but are positive for Sox2 and PSA-NCAM, identifying them as committed neuroblasts. On the basis of the marker gene expression and distinct cell morphologies, we propose a classification for the dividing cell states at the zebrafish adult telencephalic VZ.
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe in the Helmholtz Association, Karlsruhe Institute of Technology, Postfach 3640, 76021 Karlsruhe, Germany.
Martin
M
März
Prisca
P
Chapouton
Nicolas
N
Diotel
Colette
C
Vaillant
Birgit
B
Hesl
Masanari
M
Takamiya
Chen Sok
CS
Lam
Olivier
O
Kah
Laure
L
Bally-Cuif
Uwe
U
Strähle
D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020547:Lateral Ventricles; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D055747:SOX Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000145:classification / Q000166:cytology* / Q000502:physiology; D013687:Telencephalon / Q000166:cytology* / Q000502:physiology; D015027:Zebrafish / Q000033:anatomy & histology* / Q000502:physiology; D029961:Zebrafish Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D055747:SOX Transcription Factors; D012794:Sialic Acids; C544611:Sox2 protein, zebrafish; D029961:Zebrafish Proteins; C103469:polysialyl neural cell adhesion molecule