Determination of biogenic amines in Korean traditional fermented soybean paste (Doenjang).
Biogenic amines, produced by bacterial decarboxylation of amino acids, have been associated with toxicological symptoms in food products. Twenty-three samples of traditionally available Korean fermented soybean paste samples (Doenjang) were analyzed in order to determine the content of biogenic amines. Amines were extracted with 0.4M perchloric acid and derivatized with dansyl chloride. Nine biogenic amines were separated from Doenjang samples by high performance liquid chromatography using gradient elution (acetonitrile and ammonium acetate), and detected with spectrophotometric UV-vis detection at 254 nm. The pH value of all the samples was ranged from 4.8 to 6.0, and the strong amino acid decarboxylase activity was found to be in an acidic environment. The mean values of biogenic amines (tryptamine, 2-phenyl-ethylamine, putrescine, cadaverine, agmatine, histamine, tyramine, spermidine and spermine) determined in 23 Doenjang samples were found to be 18.37, 82.03, 70.84, 34.24, 47.32, 26.79, 126.66, 74.41 and 244.36 mg%, respectively. The findings of this study enhance the safety of not only Doenjang but other salted and/or fermented food products.
Shukla
Shruti
S
Department of Food Science and Technology, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea.
Park
Hae-Kyong
HK
Kim
Jong-Kyu
JK
Kim
Myunghee
M
D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005504:Food Analysis; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007723:Korea; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*
D001679:Biogenic Amines