Caveolin-1 exists and may function in cardiomyocytes.
Whether ventricular cardiac myocytes of mouse contain caveolin-1 is disputed. It has been claimed to be exclusively in nearby endothelial cell profiles. Recently, matrix metalloproteinase-2 (MMP-2) was reported to be present in mouse ventricular cardiac myocytes, colocalized with caveolin-1, and caveolin-1 knockout was found to cause the loss of MMP-2 from mouse ventricular cardiac myocytes and affect their functioning. To resolve this dispute, we labeled cardiac myocytes with caveolin-1 and endothelial cells with caveolin-2. Caveolin-2 is agreed to be present exclusively in endothelial cells. The results showed that mouse ventricular myocytes were labeled with caveolin-1 antibodies independently of any caveolin-2 labeling, and endothelial cells were labeled with both caveolin-1 and caveolin-2 antibodies. This confirms that caveolin-1 is present in mouse ventricular cardiac myocytes as well as endothelial cells. Previous evidence confirms that loss of caveolin-1 affects the function of mouse ventricular cardiac myocytes and suggests that MMP-2 may be involved.
Department of Pharmacology, Medical Sciences Building, University of Alberta, Edmonton, AB T6G2H7, Canada.
Woo Jung
WJ
Cho
Ava K
AK
Chow
Richard
R
Schulz
Edwin E
EE
Daniel
D000818:Animals; D051242:Caveolin 1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051243:Caveolin 2 / Q000032:analysis; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D006352:Heart Ventricles / Q000737:chemistry / Q000166:cytology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D032383:Myocytes, Cardiac / Q000737:chemistry* / Q000166:cytology / Q000502:physiology*
D051242:Caveolin 1; D051243:Caveolin 2