Effect of a single-use toothbrush on plaque microflora.
OBJECTIVE
To study and compare the microbial flora of dental plaque after the use of a self-contaminated toothbrush and that of a single-use toothbrush.


METHODS
The study group included 40 young volunteers from Yenepoya Dental College, who were free from any systemic or oral disease. In these subjects, plaque samples were collected after 1 month use of a self-contaminated toothbrush. Each subject was given a set of 30 new toothbrushes and a toothpaste tube and instructed to use one toothbrush everyday and discard it after use. The plaque samples were collected on a weekly interval and cultured on Mitis Salivarius agar. The colonies were identified and speciated and their count was recorded.


RESULTS
Streptococcus mitis, S. mutans, S. sanguis, S. milleri and Candida were recovered from the samples. A highly significant decrease in their numbers was found after the use of a single-use toothbrush (P value 0.001).


CONCLUSIONS
As a contaminated toothbrush can reintroduce microorganisms into the oral cavity, it may be a sound practice to change the toothbrush as frequently as possible.
Department of Microbiology, Yenepoya Medical College, Mangalore, Karnataka, India.
Vidya
V
Pai
D000293:Adolescent; D000328:Adult; D002175:Candida / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D013291:Streptococcus / Q000302:isolation & purification*; D034364:Streptococcus milleri Group / Q000302:isolation & purification; D034361:Streptococcus mitis / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000302:isolation & purification; D013298:Streptococcus sanguis / Q000302:isolation & purification; D014099:Toothbrushing / Q000295:instrumentation*; D055815:Young Adult