Advances in imaging RNA in plants.
Increasing evidence shows that many RNAs are targeted to specific locations within cells, and that RNA-processing pathways occur in association with specific subcellular structures. Compartmentation of mRNA translation and RNA processing helps to assemble large RNA-protein complexes, while RNA targeting allows local protein synthesis and the asymmetric distribution of transcripts during cell polarisation. In plants, intercellular RNA trafficking also plays an additional role in plant development and pathogen defence. Methods that allow the visualisation of RNA sequences within a cellular context, and preferably at subcellular resolution, can help to answer important questions in plant cell and developmental biology. Here, we summarise the approaches currently available for localising RNA in vivo and address the specific limitations inherent with plant systems.
Christensen
Nynne M
NM
Biosystems Department, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, 4000 Roskilde, Denmark.
Oparka
Karl J
KJ
Tilsner
Jens
J
D005821:Genetic Techniques; D008164:Luminescent Proteins / Q000032:analysis; D059828:Plant Cells; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000032:analysis*
D008164:Luminescent Proteins; D018749:RNA, Plant