Expression of recombinant ADAMTS in insect cells.
The "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) enzymes are secreted proteinases involved in development, blood clotting and the turnover of extracellular matrix. Manufacturing recombinant enzyme presents quite a challenge due to the presence of disulphide bridges, the large size and modular structure. A sub-group of these enzymes are known as "aggrecanases" and it is likely that they are involved in a number of pathologies related to increased turnover of the extracellular matrix, particularly in tissues where the concentration of proteoglycans is high, such as cartilage and the central nervous system. We have expressed three of these enzymes, ADAMTS-1, -4 and -5, in insect cells using plasmid-based systems.
School of Biological Sciences, University of East Anglia, Norwich, UK.
Gavin C
GC
Jones
Mireille N
MN
Vankemmelbeke
David J
DJ
Buttle
D051722:ADAM Proteins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics; D007313:Insecta / Q000166:cytology*; D008967:Molecular Biology / Q000379:methods*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014162:Transfection
D011994:Recombinant Proteins; D051722:ADAM Proteins