Tissue engineering approach to repair abdominal wall defects using cell-seeded bovine tunica vaginalis in a rabbit model.
The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.
Ayele
T
T
Faculty of Veterinary Medicine, University Putra Malaysia, 43400, Serdang, Selangor, Darul Ehsan, Malaysia. ayutadei@yahoo.com
Zuki
A B Z
AB
Noorjahan
B M A
BM
Noordin
M M
MM
D034861:Abdominal Wall / Q000601:surgery*; D000818:Animals; D002417:Cattle; D002477:Cells; D003094:Collagen; D005109:Extracellular Matrix; D020139:Gastroschisis / Q000601:surgery; D008297:Male; D032446:Myoblasts; D011817:Rabbits; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
D003094:Collagen