Regulation of IL-9 expression by IL-25 signaling.
The physiological regulation of the expression of interleukin (IL)-9, a cytokine traditionally regarded as being T(H)2 associated, remains unclear. Here, we show that IL-9-expressing T cells generated in vitro in the presence of transforming growth factor-beta and IL-4 express high levels of mRNA for IL-17 receptor B (IL-17RB), the receptor for IL-25. Treatment of these cells with IL-25 enhances IL-9 expression in vitro. Moreover, transgenic and retroviral overexpression of IL-17RB in T cells results in IL-25-induced IL-9 production that is IL-4 independent. In vivo, the IL-25-IL-17RB pathway regulates IL-9 expression in allergic airway inflammation. Thus, IL-25 is a newly identified regulator of IL-9 expression.
Department of Immunology, University of Texas and MD Anderson Cancer Center, Houston, Texas, USA.
Pornpimon
P
Angkasekwinai
Seon Hee
SH
Chang
Manoj
M
Thapa
Hiroshi
H
Watarai
Chen
C
Dong
D000818:Animals; D016535:Bronchial Hyperreactivity / Q000235:genetics / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002452:Cell Count; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D016906:Interleukin-9 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D053722:Receptors, Interleukin-17 / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*
D016906:Interleukin-9; D007378:Interleukins; C442281:Mydgf protein, mouse; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17