Improvement on the performance of bone regeneration of calcium sulfate hemihydrate by adding mineralized collagen.
Comparative investigations of bone regeneration performance for calcium sulfate hemihydrate (CaSO(4).(1/2)H(2)O; CSH) only and CSH with mineralized collagen are reported in this article. The mineralized collagen is the nanohydroxyapatite/collagen (nHAC). The investigations included biocompatibility in vitro and performance of bone repair in vivo. Quantitative and qualitative biocompatibility assays with bone stromal stem cells were performed. A critical box-shaped defect model in the mandible of the rabbit was used to evaluate the bone-remodeling ability of CSH and nHAC/CSH. Results in vitro indicated that the nHAC/CSH significantly improved bioactivity compared with that of CSH, especially in promoting cell adhesion. Further, a higher bone remodeling activity was observed around nHAC/CSH composite than the CSH, especially at the early stage of remodeling. This result means that nHAC/CSH could cause an earlier accelerator and better osseointegration for bone repair than CSH only.
Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang, China.
Huan-Ye
HY
Liu
Xi
X
Liu
Li-Ping
LP
Zhang
Hong-Jun
HJ
Ai
Fu-Zhai
FZ
Cui
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016723:Bone Remodeling / Q000187:drug effects*; D002133:Calcium Sulfate / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D001672:Biocompatible Materials; C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite; D002133:Calcium Sulfate