[Analysis of serum protein fingerprints on non-small cell lung cancer lymphatic metastasis].
OBJECTIVE
To establish a classification model and serum proteomic patterns in non-small cell lung cancer (NSCLC) patients with lymph node metastasis by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS).


METHODS
The relative contents of serum proteins of 84 NSCLC patients with different N stages (35 N0 cases, 19 N1 and 30 N2 respectively) were detected by CM10 chip and SELDI-TOF-MS; two decision trees were generated to distinguish lymph nodes metastasis (N0 versus N1 + N2) and mediastinal lymph nodes metastasis (N0 + N1 versus N2) respectively.


RESULTS
The model in which 50 patients were randomly chosen differentiated patients with lymph nodes metastasis from N0 patients with a sensitivity of 96.3%(26/27) and a specificity of 95.7%(22/23) in the training set, a following blind test was taken. Subsequently, compared with 49 patients with lymph node metastasis (N1 + N2), 15 patients with total negative lymph nodes (including lobar, segmental and subsegmental nodes necessarily) were defined as "true" N0 and were chosen to form a better predictive model with a 77.6% (38/49) sensitivity and a 93.3% (14/15) specificity respectively. And 6682.0Da, together with other five proteins, had significant difference between two groups; the result of this model for distinguishing the mediastinal lymph nodes metastasis is more accurate than thoracic CT analyses by Alongi F and many other clinical centers. It had a sensitivity of 80.0% (24/30) and a specificity of 77.8% (42/54) respectively.


CONCLUSIONS
SELDI-TOF-MS showed a potential value for predicting lymph nodes metastasis in NSCLC patients. And further studies are required to confirm the models and identify the related proteins.
Lü
Chao
C
Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery, Peking University School of Clinical Oncology, Beijing Cancer Hospital and Institute, Beijing, China.
Shen
Jing
J
Wu
Nan
N
Zheng
Qing-feng
QF
Wang
Jia
J
Yan
Shi
S
Feng
Yuan
Y
Yang
Yue
Y
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010449:Peptide Mapping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins