T lymphocytes mediate hypertension and kidney damage in Dahl salt-sensitive rats.
This study examined mechanisms by which immune cells participate in the development of hypertension and renal disease in Dahl salt-sensitive (SS) rats. Increasing dietary salt from 0.4% to 4.0% NaCl significantly increased renal infiltration of T lymphocytes from 8.8 +/- 1.2 x 10(5) to 14.4 +/- 2.0 x 10(5) cells/2 kidneys, increased arterial blood pressure from 131 +/- 2 to 165 +/- 6 mmHg, increased albumin excretion rate from 17 +/- 3 to 129 +/- 20 mg/day, and resulted in renal glomerular and tubular damage. Furthermore, renal tissue ANG II was not suppressed in the kidneys of SS rats fed 4.0% NaCl. Administration of the immunosuppressive agent mycophenolate mofetil (MMF; 20 mg.kg(-1).day(-1)) prevented the infiltration of T lymphocytes and attenuated Dahl SS hypertension and renal disease. In contrast to vehicle-treated rats, Dahl SS rats administered MMF demonstrated a suppression of renal tissue ANG II from 163 +/- 26 to 88 +/- 9 pg/g of tissue when fed high salt. Finally, it was demonstrated that the T lymphocytes isolated from the kidney possess renin and angiotensin-converting enzyme activity. These data indicate that infiltrating T cells are capable of participating in the production of ANG II and are associated with increased intrarenal ANG II, hypertension, and renal disease. The suppression of T-cell infiltration decreased intrarenal ANG II and prevented Dahl SS hypertension and kidney damage. As such, infiltrating cells are capable of participating in the established phase of Dahl SS hypertension.
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Carmen
C
De Miguel
Satarupa
S
Das
Hayley
H
Lund
David L
DL
Mattson
D000804:Angiotensin II / Q000097:blood; D000818:Animals; D001794:Blood Pressure / Q000276:immunology; D006973:Hypertension / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D007668:Kidney / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D007684:Kidney Tubules / Q000276:immunology / Q000473:pathology; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D051381:Rats; D020303:Rats, Inbred Dahl / Q000502:physiology; D012083:Renin / Q000097:blood; D012709:Serum Albumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D012709:Serum Albumin; D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A; D012083:Renin