In vitro stability of Cucumber mosaic virus nanoparticles carrying a Hepatitis C virus-derived epitope under simulated gastrointestinal conditions and in vivo efficacy of an edible vaccine.
The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intestinal conditions. Then the possibility of inducing a humoral immune response in rabbits fed with R9-CMV infected lettuce plants was demonstrated, suggesting that this system could function as a confirming tool of a bioreactor for the production of a stable edible vaccine against HCV.
Nuzzaci
M
M
Department of Biology, Plant Protection and Agrobiotechnology, University of Basilicata, 85100 Potenza, Italy.
Vitti
A
A
Condelli
V
V
Lanorte
M T
MT
Tortorella
C
C
Boscia
D
D
Piazzolla
P
P
Piazzolla
G
G
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D017799:Cucumovirus / Q000737:chemistry* / Q000235:genetics; D000939:Epitopes / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D016174:Hepacivirus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000517:prevention & control*; D018937:Hepatitis C Antibodies / Q000097:blood; D007422:Intestines; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D016865:Reassortant Viruses / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D013270:Stomach; D022462:Vaccines, Edible / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D014771:Virion / Q000276:immunology
D000939:Epitopes; D018937:Hepatitis C Antibodies; D010455:Peptides; D022462:Vaccines, Edible; D014761:Viral Hepatitis Vaccines