Experimental autoimmune encephalomyelitis in the mouse.
This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.
Northwestern University, Evanston, Illinois.
Stephen D
SD
Miller
Northwestern University, Evanston, Illinois.
William J
WJ
Karpus
NIAID, NIH, Bethesda, Maryland.
Todd Scott
TS
Davidson
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000276:immunology; D011510:Proteolipids / Q000276:immunology
D004676:Myelin Basic Protein; D011510:Proteolipids