An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix.
One of the major obstacles in drug discovery is the lack of in vitro three-dimensional (3D) models that can capture more complex features of a disease.Here we established a in vitro physiological model of the metabolic syndrome (MS) using cell-assembly technique (CAT), which can assemble cells into designated places to form complex 3D structures. Adipose-derived stromal (ADS) cells were assembled with gelatin/alginate/fibrinogen. Fibrin was employed as an effective material to regulate ADS cell differentiation and self-organization along with other methods. ADS cells differentiated into adipocytes and endothelial cells, meanwhile, the cells were induced to self-organize into an analogous tissue structure. Pancreatic islets were then deposited at designated locations and constituted the adipoinsular axis with adipocytes. Analysis of the factors involved in energy metabolism showed that this system could capture more pathological features of MS. Drugs known to have effects on MS showed accordant effects in this system, indicating that the model has potential in MS drug discovery. Overall, this study demonstrated that cell differentiation and self-organization can be regulated by techniques combined with CAT. The model presented could result in a better understanding of the pathogenesis of MS and the development of new technologies for drug discovery.
Key Laboratory for Advanced Materials Processing Technology, Ministry of Education & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing, PR China. xumingen@mail.tsinghua.edu.cn
Mingen
M
Xu
Xiaohong
X
Wang
Yongnian
Y
Yan
Ri
R
Yao
Yakun
Y
Ge
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000473:pathology*; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D019332:Endothelin-1 / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects / Q000503:physiopathology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005340:Fibrinogen / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050356:Lipid Metabolism / Q000187:drug effects; D024821:Metabolic Syndrome / Q000473:pathology* / Q000503:physiopathology; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry*
D054392:Adipokines; D000464:Alginates; D019332:Endothelin-1; D005230:Fatty Acids, Nonesterified; D006603:Hexuronic Acids; D007328:Insulin; D009569:Nitric Oxide; D020723:Glucuronic Acid; D005780:Gelatin; D005340:Fibrinogen; D005947:Glucose