Renin, endothelial NO synthase and endothelin gene expression in the 2kidney-1clip Goldblatt model of long-term renovascular hypertension.
OBJECTIVE
Numerous reports have shown the influence of renin, nitric oxide (NO) and the endothelin (ET) systems for regulation of blood pressure and renal function. Furthermore, interactions between these peptides have been reported. Aim of our study was to investigate the relative contribution of these compounds in long-term renovascular hypertension / renal ischemia.


METHODS
Hypertension / left-sided renal ischemia was induced using the 2K1C-Goldblatt rat model. Renal renin, ET-1, ET-3 and endothelial NO synthase (eNOS) gene expression was measured by means of RNAse protection assay at different timepoints up to 10 weeks after induction of renal artery stenosis.


RESULTS
Plasma renin activity and renal renin gene expression in the left kidney were increased in the clipped animals while eNOS expression was unchanged. Furthermore, an increase in ET-1 expression and a decrease of ET-3 expression was detected in early stenosis.


CONCLUSIONS
While renin is obviously involved in regulation of blood pressure and renal function in unilateral renal artery stenosis, ET-1, ET-3 and endothelium derived NO do not appear to play an important role in renal adaptation processes in long-term renal artery stenosis, although ET-1 and ET-3 might be involved in short-term adaptation processes.
Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Regensburg, Germany. stephan.reinhold@klinik.uni-regensburg.de
S W
SW
Reinhold
D C
DC
Uihlein
C A
CA
Böger
S
S
Kloiber
K
K
Frölich
T
T
Bergler
B
B
Banas
F
F
Schweda
B K
BK
Krämer
D000818:Animals; D004195:Disease Models, Animal; D016232:Endothelins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006978:Hypertension, Renovascular / Q000235:genetics* / Q000378:metabolism; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000235:genetics* / Q000378:metabolism
D016232:Endothelins; D052250:Nitric Oxide Synthase Type III; D012083:Renin