Zona pellucida domain proteins remodel the apical compartment for localized cell shape changes.
The zona pellucida domain (ZPD) defines a conserved family of membrane-anchored matrix proteins that are, as yet, poorly characterized with respect to their functions during development. Using genetic approaches in flies, we show here that a set of eight ZPD proteins is required for the localized reorganization of embryonic epidermal cells during morphogenesis. Despite varying degrees of sequence conservation, these ZPD proteins exert specific and nonredundant functions in the remodeling of epidermal cell shape. Each one accumulates in a restricted subregion of the apical compartment, where it organizes local interactions between the membrane and the extracellular matrix. In addition, ZPD proteins are required to sculpture the actin-rich cell extensions and maintain appropriate organization of the apical compartment. These results on ZPD proteins therefore reveal a functional subcompartmentalization of the apical membrane and its role in the polarized control of epithelial cell shape during development.
Fernandes
Isabelle
I
Université de Toulouse, UPS, Centre de Biologie du Développement, F-31062, Toulouse, France.
Chanut-Delalande
Hélène
H
Ferrer
Pierre
P
Latapie
Yvan
Y
Waltzer
Lucas
L
Affolter
Markus
M
Payre
François
F
Plaza
Serge
S
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D016764:Cell Polarity / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D022081:Cell Surface Extensions / Q000378:metabolism / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology
D029721:Drosophila Proteins; D004527:Egg Proteins