Resveratrol increases iodide trapping in the rat thyroid cell line FRTL-5.
BACKGROUND
Resveratrol, a polyphenol found in grapes, exhibits several beneficial health effects by its antioxidant, antiinflammatory, and chemopreventive properties. The aim of the present study was to determine the effect of resveratrol on iodide trapping and efflux as well as its mode of action using FRTL-5 cells, having in mind the pivotal role of the natrium iodide symporter (NIS) in the treatment of differentiated thyroid cancers.


METHODS
Cells were treated with resveratrol for various times and doses, in the presence or absence of thyrotropin (TSH). Iodide trapping, iodide efflux, rat NIS (rNIS) protein expression, and cyclic AMP (cAMP) production were evaluated.


RESULTS
Resveratrol increased iodide trapping in a time-dependent (optimal 6 hours) and dose-dependent (100 microM) way in the presence of TSH. It showed an additive effect when concomitantly added with an optimal dose of TSH. Resveratrol (50 microM) increased (threefold) rNIS protein expression. In TSH-deprived cells, resveratrol also provoked an increase in rNIS protein (>3-fold in 6 hours) with an optimum at 40 microM. Resveratrol did not inhibit iodide efflux from FRTL-5 cells. It neither increased intracellular cAMP nor induced the arborization of living cells, two TSH-induced effects. A non-cAMP mode of action is highly suspected.


CONCLUSIONS
Resveratrol increases iodide trapping in FRTL-5 cells, increasing iodide influx and rNIS protein level even in the absence of TSH. It has an additive effect with TSH. Consequently, resveratrol could be a promising molecule for radioiodide therapy in follicular and papillary differentiated thyroid carcinoma in association with recombinant human TSH.
INSERM UMR-911 and Research Center in Biological and Oncopharmacological Oncology (CrO2), School of Medicine-Timone, Aix-Marseille University, France.
Hichem
H
Sebai
Sonia
S
Hovsépian
Elodie
E
Ristorcelli
Ezzedine
E
Aouani
Dominique
D
Lombardo
Guy
G
Fayet
D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D007454:Iodides / Q000378:metabolism*; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D027981:Symporters / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology; D000099209:Sodium-Iodide Symporters
D000242:Cyclic AMP; D007454:Iodides; D000077185:Resveratrol; D013267:Stilbenes; D027981:Symporters; D013972:Thyrotropin; D000099209:Sodium-Iodide Symporters