A competitive indirect enzyme-linked immunoassay for lead ion measurement using mAbs against the lead-DTPA complex.
Immunoassays for quantitative measurement of environmental heavy metals offer several advantages over other traditional methods. To develop an immunoassay for lead, Balb/c mice were immunized with a lead-chelate-protein conjugate to allow maximum exposure of the metal to the immune system. Three stable hybridoma cell lines were obtained through spleen cells fusion with Sp2/0 cells. One cell line, 2A11D11, produced mAbs with preferential selectivity and sensitivity for Pb-DTPA than DTPA, exhibiting an affinity constant of 3.34 + or - 0.24 x 10(9) M(-1). Cross reactivity (CR) with other metals were below 1%, except for Fe(III) with a CR less than 5%. This quantitative indirect ELISA for the lead ion was used to detect environmental lead content in local water sources; importantly, the results from the immunoassay were in excellent agreement with those from ICP-MS. Development of immunoassays for metal ions may thus facilitate the detection and regulation of environmental pollution.
Xiang
Jun-jian
JJ
Molecular Immunology and Antibody Engineering Center, Jinan University, Guangzhou 510632, China.
Zhai
Yi-fan
YF
Tang
Yong
Y
Wang
Hong
H
Liu
Bin
B
Guo
Chang-wei
CW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002460:Cell Line; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007854:Lead / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D004369:Pentetic Acid / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants; D007854:Lead; D004369:Pentetic Acid