Variation of genes encoding GGPLs syntheses among Mycoplasma fermentans strains.
The information of the biosynthesis pathways of Mycoplasma fermentans specific major lipid-antigen, named glycoglycerophospholipids (GGPLs), is expected to be some of help to understand the virulence of M. fermentans. We examined primary structure of cholinephosphotransferase (mf1) and glucosyltransferase (mf3) genes, which engage GGPL-I and GGPL-III synthesis, in 20 strains, and found four types of variations in the mf1 gene but the mf3 gene in two strains was not detected by PCR. These results may have important implications in virulence factor of M. fermentans.
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Japan.
Masatoshi
M
Fujihara
Noriko
N
Ishida
Kozo
K
Asano
Kazuhiro
K
Matsuda
Nobuo
N
Nomura
Yoshihiro
Y
Nishida
Ryô
R
Harasawa
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D017931:DNA Primers; D002798:Diacylglycerol Cholinephosphotransferase / Q000235:genetics; D014644:Genetic Variation; D006017:Glycolipids / Q000235:genetics*; D016858:Mycoplasma fermentans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
C106372:1''-phosphocholine-2''-aminodihydroxypropane-3''-phospho-6'-glucopyranosyl-(1'-3)-1,2-dipalmitoylglycerol; D000942:Antigens, Bacterial; D017931:DNA Primers; D006017:Glycolipids; C091226:6'-O-phosphocholineglucopyranosyl-(1'-3)-1,2-dipalmitoyl; D002798:Diacylglycerol Cholinephosphotransferase