Identification, bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa.
Messenger RNA-like non-coding RNAs (mlncRNAs) are a newly identified group of non-coding RNAs (ncRNAs) that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.
Xiao
Bang
B
Huazhong Agricultural University, Wuhan, China.
Zhang
Xingju
X
Li
Yong
Y
Tang
Zhonglin
Z
Yang
Shulin
S
Mu
Yulian
Y
Cui
Wentao
W
Ao
Hong
H
Li
Kui
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000145:classification; D017423:Sequence Analysis, RNA; D034421:Sus scrofa / Q000235:genetics*
D012313:RNA