The effects of selective and nonselective phosphodiesterase inhibitors on phagocytic cells in mice.
Milrinone (1 mg/kg i.m.), sildenafil (1 mg/kg p.o), and aminophylline (20 mg/kg i.m.) were administered to mice once or five times. The drugs increased the production of IL-1beta and NO by peritoneal macrophages. Milrinone or aminophylline did not change the percentage of phagocytosing cells. A single administration of sildenafil increased the percentage of phagocytosing granulocytes (after 12 h). Sildenafil administered five times decreased the percentage of phagocytosing monocytes (72 h after the last dose). A single administration of the drugs did not change the oxidative burst activity. PDE inhibitors administered five times temporarily enhanced the percentage of cells producing reactive oxidants.
Szczypka
Marianna
M
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. marianna.szczypka@up.wroc.pl
Obmińska-Mrukowicz
Bozena
B
D000628:Aminophylline / Q000494:pharmacology*; D000818:Animals; D005260:Female; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D020105:Milrinone / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects; D009569:Nitric Oxide / Q000032:analysis; D010586:Phagocytes / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology*
D053583:Interleukin-1beta; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000628:Aminophylline; D009569:Nitric Oxide; D000068677:Sildenafil Citrate; D020105:Milrinone