[Effects of occupational microwave irradiation on heat shock protein 70 expressions in rat hippocampus].
OBJECTIVE
To study the change of heat shock protein (HSP)70 expression after exposure to occupational microwave in rats hippocampus, and explore the role of HSP70 in the mechanism of bio-effect of microwave irradiation.


METHODS
The animal model was established by whole body exposures in 90, 5 W/cm(2) microwave irradiation field for 20 min in rats. Changes of the mRNA of hsp70 expressions in rat hippocampus at different time were studied by RT-PCR, and the protein change by Western blot.


RESULTS
The mRNA and protein expression of hsp70 in rat hippocampus increased after 90 W/cm(2) and 5 W/cm(2) microwave irradiation for 20 min. The anal temperature and the value of SAR increased significantly. These changes were positively correlated with power and irradiation time of microwave. The results indicated that microwave irradiation led to HSP70 syntheses effectively.


CONCLUSIONS
Microwave irradiation can obviously induce the thermal effect and activate HSP70, and initiate the endogenous protective mechanism of central nervous system.
Department of Military Occupational Hygiene, Third Military Medical University, Chongqing 400038, China.
Yu-tong
YT
Hao
Li-ping
LP
Pei
Chun-hai
CH
Chen
Xue-sen
XS
Yang
Guang-bin
GB
Zhang
Zhao-hui
ZH
Deng
Zheng-ping
ZP
Yu
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism* / Q000528:radiation effects; D008872:Microwaves / Q000009:adverse effects*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger