Establishment and characterization of dengue virus type 2 nonstructural protein 1 specific T cell lines.
Immunity against dengue viruses (DENV) infection may include cellular immune responses which involve in the immunopathology of DENV infection hosts. This study was to establish short-term dengue virus type 2 (DENV2) nonstructural protein 1 (NS1) specific T cells from splenocytes from BALB/c mice immunized with DENV2 NS1 in vitro, which may be used to identify immunopathologic mechanism of dengue. Nine DENV2 NS1 specific T cell lines were successfully established by using limiting dilution methods and maintained for 20 weeks by re-stimulated with DENV2 NS1, recombinant mouse IL-2 and antigen presenting cell weekly. Phenotypically, these cells were mainly composed of CD3(+)CD4(+) T cells. The culture supernatants of these cells contained large amounts of TNF-α and IFN-γ. Vascular tissue pathological change could be found in the mice adoptive transferred with DENV2 NS1 specific T cells. The results indicate that DENV2 NS1 specific T cells could be established and maintained with syngeneic T cell growth factors in vitro. Meanwhile, DENV2 NS1 specific T cells might contribute to the immunopathology of vascular leakage of dengue.
Xiao-meng
Yang
Y
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-Sen University, 74#, Zhongshan 2nd Road, Guangzhou, 510080, China.
Li-fang
Jiang
J
Yun-xia
Tang
T
Yue
Yin
Y
Wen-quan
Liu
L
Dan-yun
Fang
F
D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D003715:Dengue / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005434:Flow Cytometry; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D017361:Viral Nonstructural Proteins / Q000276:immunology*
D000956:Antigens, Viral; D007376:Interleukin-2; C079340:NS1 protein, Dengue virus type 2; D014409:Tumor Necrosis Factor-alpha; D017361:Viral Nonstructural Proteins; D007371:Interferon-gamma