A systemic proteomic analysis of Populus chloroplast by using shotgun method.
The chloroplast is one of the most important organelles in plants. Proteomic investigations of chloroplasts have been undertaken for many herb plant species, but to date no such investigation has been reported for woody plant chloroplasts. In the present study we initiated a systematic proteomic study of Populus chloroplasts using a shotgun proteomic method. After isolation of chloroplasts and tryptic digestion of the proteins, the protein fragments were separated via HPLC using an SCX column, and the peptides were analyzed by LC-MS/MS; 119 proteins were successfully identified. Based on annotation information in the UniProtKB/Swiss-Prot database, these proteins were identified as being localized in the chloroplast thylakoid membrane, chloroplast stroma, chloroplast thylakoid lumen, and plastoglobules. Over 50% of all identified proteins were confirmed as chloroplast thylakoid proteins, and 85 are encoded by the chloroplast genome with the remaining proteins encoded by the nuclear genome. Based on functional annotation, these proteins were classified into four functional categories, including photosynthesis, redox regulation and stress, primary and secondary metabolism, transport and signaling. These data provide a valuable basis for further studies on photosynthesis in poplar species.
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Hong-Mei
HM
Yuan
Kai-Long
KL
Li
Rui-Juan
RJ
Ni
Wen-Dong
WD
Guo
Zhuo
Z
Shen
Chuan-Ping
CP
Yang
Bai-Chen
BC
Wang
Gui-Feng
GF
Liu
Chang-Hong
CH
Guo
Jing
J
Jiang
D002736:Chloroplasts / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D016208:Databases, Factual; D057166:High-Throughput Screening Assays / Q000379:methods; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D032107:Populus / Q000737:chemistry* / Q000166:cytology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
D010940:Plant Proteins; D020543:Proteome