High complexity of Plasmodium vivax infections in symptomatic patients from a rural community in central Vietnam detected by microsatellite genotyping.
Fourteen published and three newly identified polymorphic microsatellites were used to genotype 69 Plasmodium vivax samples obtained from 39 patients detected over a period of two years who lived in a rural community of central Vietnam. All samples were polyclonal with an average expected heterozygosity of 0.86. Among the 39 patients, 16 experienced 1-5 recurrent episodes of P. vivax malaria, most of them (83%) with a different genotype profile compared with previous infections. The minimal set of microsatellites required for differentiating the genotype profiles of the recurrent infections compared with the full set of 17 microsatellites was explored. A combination of five markers was sufficient to identify all recurrent infections with an unrelated or different genotype profile compared with all previous episodes.
Department of Parasitology, Institute of Tropical Medicine, Nationalestraat 155, Antwerp, Belgium. pvandeneede@itg.be
Peter
P
Van den Eede
Annette
A
Erhart
Gert
G
Van der Auwera
Chantal
C
Van Overmeir
Ngo Duc
ND
Thang
Le Xuan
le X
Hung
Jozef
J
Anné
Umberto
U
D'Alessandro
D000293:Adolescent; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D018895:Microsatellite Repeats; D010966:Plasmodium vivax / Q000235:genetics*; D014744:Vietnam / Q000453:epidemiology; D055815:Young Adult