CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics.
Owing to its availability, ease of collection, and correlation with pathophysiology of diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited clinical impact, due to factors such as modest numbers of subjects, absence of disease controls, small numbers of defined biomarkers, and diversity of analytical platforms. Therefore, it is difficult to merge biomarkers from different studies into a broadly applicable human urinary proteome database. Ideally, the methodology for defining the biomarkers should combine a reasonable analysis time with high resolution, thereby enabling the profiling of adequate samples and recognition of sufficient features to yield robust diagnostic panels. Capillary electrophoresis coupled to mass spectrometry (CE-MS), which was used to analyze urine samples from healthy subjects and patients with various diseases, is a suitable approach for this task. The database of these datasets compiled from the urinary peptides enabled the diagnosis, classification, and monitoring of a wide range of diseases. CE-MS exhibits excellent performance for biomarker discovery and allows subsequent biomarker sequencing independent of the separation platform. This approach may elucidate the pathogenesis of many diseases, and better define especially renal and urological disorders at the molecular level.
Coon
Joshua J
JJ
Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Zürbig
Petra
P
Dakna
Mohammed
M
Dominiczak
Anna F
AF
Decramer
Stéphane
S
Fliser
Danilo
D
Frommberger
Moritz
M
Golovko
Igor
I
Good
David M
DM
Herget-Rosenthal
Stefan
S
Jankowski
Joachim
J
Julian
Bruce A
BA
Kellmann
Markus
M
Kolch
Walter
W
Massy
Ziad
Z
Novak
Jan
J
Rossing
Kasper
K
Schanstra
Joost P
JP
Schiffer
Eric
E
Theodorescu
Dan
D
Vanholder
Raymond
R
Weissinger
Eva M
EM
Mischak
Harald
H
Schmitt-Kopplin
Philippe
P