Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes.
Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lss, IFN-gamma, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-gamma) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-gamma/CCR5 and IL-1ss/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro medico Nacional Siglo XXI, México. srdotor@yahoo.com.mx
Sara
S
Rojas-Dotor
Julia
J
Pérez-Ramos
Juan Antonio
JA
Giménez-Scherer
Francisco
F
Blanco-Favela
Guadalupe
G
Rico-Rosillo
D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004748:Entamoeba histolytica / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009842:Oligopeptides / Q000096:biosynthesis / Q000494:pharmacology*; D054398:Receptors, CCR4 / Q000187:drug effects* / Q000276:immunology; D019713:Receptors, CCR5 / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
D016207:Cytokines; D009842:Oligopeptides; D054398:Receptors, CCR4; D019713:Receptors, CCR5; C430647:monocyte locomotion inhibitory factor; D013755:Tetradecanoylphorbol Acetate