Characterization of the flounder IL-6 promoter and its regulation by the p65 NF-kappaB subunit.
Interleukin (IL)-6 plays important roles in the regulation of the immune response and inflammation in many cell types and its induction by bacterial endotoxins or cytokines is regulated at the transcriptional level. The present study demonstrates the isolation and characterization of the flounder IL-6 promoter sequence and its transcriptional regulation in olive flounder (hirame) natural embryo (HINAE) cells. The promoter region (-400 to +79 bp from the transcription initiation site) of the flounder IL-6 gene contains putative cis-regulatory elements for CCAAT-enhancer-binding proteins (C/EBP; -346 to -355 bp and -166 to -160 bp), cAMP response element binding (CREB; -81 to -85 bp), the activator protein 1 (AP-1; -56 to -62 bp), and NF-kappaB (-39 to -48 bp). Transfection of p65 stimulated the PoIL-6-luc-WT reporter, but not the PoIL-6-luc-kappaB mt reporter, and treatment with LPS augmented p65-stimulated reporter activity in HINAE cells. In contrast, transfection of C/EBPbeta or c-Jun failed to induce synergistic effects in the LPS-driven PoIL-6-luc-WT reporter activity. These results give us new insight into the regulation of flounder IL-6 transcription by the p65 NF-kappaB subunit.
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 619-705, Republic of Korea. heejkong@hotmail.com
Hee Jeong
HJ
Kong
Bo-Hye
BH
Nam
Young-Ok
YO
Kim
Woo-Jin
WJ
Kim
Hyun Kook
HK
Cho
Chang Hoon
CH
Lee
Sang-Jun
SJ
Lee
Kyung-Kil
KK
Kim
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005432:Flounder / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051996:Transcription Factor RelA / Q000235:genetics* / Q000276:immunology*; D014162:Transfection
D000276:Adjuvants, Immunologic; D015850:Interleukin-6; D008070:Lipopolysaccharides; D051996:Transcription Factor RelA