Estradiol or fluoxetine alters depressive behavior and tryptophan hydroxylase in rat raphe.
The effects of 17beta-estradiol and fluoxetine on behavior of ovariectomized rats subjected to the forced swimming test and the expression of tryptophan hydroxylase (TPH) in dorsal and median raphe were investigated, respectively through time sampling technique of behavior scoring and immunohistochemistry. Both estradiol and fluoxetine increased swimming and decreased immobility in the forced swimming test. The forced swimming stress decreased integrated optical density of TPH-positive regions in dorsal and median raphe. Both estradiol and fluoxetine administration prevented integrated optical density of TPH-positive regions from being decreased by forced swimming stress. These observations suggest that both estradiol and fluoxetine have protective bearing on ovariectomized rats enduring forced swimming stress.
Department of Psychiatry, Huashan Hospital, Fudan University, Shanghai, PR China.
Fu-Zhong
FZ
Yang
Yan
Y
Wu
Wei-Guo
WG
Zhang
Yi-Yun
YY
Cai
Shen-Xun
SX
Shi
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D004967:Estrogens / Q000008:administration & dosage / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D005473:Fluoxetine / Q000008:administration & dosage / Q000494:pharmacology*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D010052:Ovariectomy; D011903:Raphe Nuclei / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017367:Selective Serotonin Reuptake Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*; D013550:Swimming; D014365:Tryptophan Hydroxylase / Q000378:metabolism*
D004958:Estradiol; D004967:Estrogens; D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors; D014365:Tryptophan Hydroxylase