Citrus limetta leaves extract antagonizes the hypertensive effect of angiotensin II.
BACKGROUND
Citrus limetta Risso (Rutaceae) is widely used in Mexico for healing purposes, among them as antihypertensive treatment.


OBJECTIVE
To assess the antihypertensive effect of C. limetta leaves as one of its ethnomedical uses.


METHODS
The acute response of blood pressure to angiotensin II administration was measured in mice. Additionally, the acute oral toxicity profiles were determined.


RESULTS
The findings of the current investigation showed that different concentrations of the aqueous extract prevented the raise of systolic blood pressure (p< or =0.001 vs. vehicle), diastolic blood pressure (p< or =0.0002 vs. vehicle) and mean blood pressure (p< or =0.0000 vs. vehicle); with a dose dependent effect for diastolic pressures at 125-500mg/kg dosages. The 500 and 1000mg/kg doses inhibited the action of Ang II in similar extent to telmisartan. Toxic signs or deaths were not observed in mice treated at 2000mg/kg of C. limetta extract.


CONCLUSIONS
All doses of C. limetta aqueous extract, used in this assay, were safe and effective.
Perez
Yolanda Y
YY
Centro de Investigacion Biomedica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Centro, 62790, Xochitepec, Morelos, Mexico. perezyy@yahoo.com
Jimenez-Ferrer
Enrique
E
Alonso
Daniel
D
Botello-Amaro
Carol A
CA
Zamilpa
Alejandro
A
D000804:Angiotensin II / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D001562:Benzimidazoles; D001565:Benzoates; D001794:Blood Pressure / Q000187:drug effects*; D002957:Citrus / Q000187:drug effects; D002986:Clinical Trials as Topic; D004304:Dosage Forms; D006801:Humans; D006973:Hypertension / Q000188:drug therapy*; D008297:Male; D008800:Mexico; D051379:Mice; D008813:Mice, Inbred ICR; D018515:Plant Leaves / Q000187:drug effects; D011897:Random Allocation; D000077333:Telmisartan
D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D004304:Dosage Forms; D000804:Angiotensin II; D000077333:Telmisartan