Investigation of possible horizontal gene transfer from transgenic rice to soil microorganisms in paddy rice field.
In order to monitor the possibility of horizontal gene transfer between transgenic rice and microorganisms in paddy rice field, the gene flow from bifunctional fusion (TPSP) rice containing trehalose-6-phosphate synthase and phosphatase to microorganisms in soils was investigated. The soil samples collected every month from the paddy rice field during June, 2004 to March, 2006 were investigated by multiplex PCR, Southern hybridization, and amplified fragment length polymorphism (AFLP). The TPSP gene from soil genomics DNAs was not detected by PCR. Soil genomic DNAs were not shown its homologies on the Southern blotting data, indicating that gene-transfer did not occur during the last two years in paddy rice field. In addition, the AFLP band patterns produced by both soil genomic DNAs extracted from transgenic and non-transgenic rice field appeared similar to each other when analyzed by NTSYSpc program. Thus, these data suggest that transgenic rice does not give a significant impact on the communities of soil microorganisms although long-term observation may be needed.
Kim
Sung Eun
SE
Department of Food Science and Technology, Kyungpook National University, Daegu 702-701, Korea.
Moon
Jae Sun
JS
Kim
Jung Kyu
JK
Choi
Won Sik
WS
Lee
Sang Han
SH
Kim
Sung Uk
SU
D022761:Gene Transfer, Horizontal; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D010940:Plant Proteins; D012987:Soil; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase