Ant fat extraction with a Soxhlet extractor.
Stored fat can be informative about the relative age of an ant, its nutritional status, and the nutritional status of the colony. Several methods are available for the quantification of stored fat. Before starting a project involving fat extraction, investigators should weigh the advantages and disadvantages of different methods in order to choose the one that is best suited to the question being addressed. This protocol, although not as accurate as some alternatives, facilitates the rapid quantification of many individuals.
School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. csmith38@asu.edu
Chris R
CR
Smith
Walter R
WR
Tschinkel
D000273:Adipose Tissue / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D001000:Ants / Q000033:anatomy & histology / Q000502:physiology*; D001695:Biology / Q000379:methods*; D004867:Equipment Design; D007756:Laboratory Animal Science / Q000379:methods*