Transient up-regulation of immunity- and apoptosis-related genes in Caco-2 cells cocultured with THP-1 cells evaluated by DNA microarray analysis.
We analyzed changes in gene expression in Caco-2 cells cocultured with THP-1 cells over time periods of 0, 1, 3, 6, 24, and 48 h using a DNA microarray. Differentially expressed genes extracted with maSigPro indicated that an early defense response and cell death occured at the same time, causing an inflammatory condition.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. sabuyoko43@hotmail.com
Yoko
Y
Ishimoto
Yuji
Y
Nakai
Hideo
H
Satsu
Mamoru
M
Totsuka
Makoto
M
Shimizu
D017209:Apoptosis / Q000235:genetics*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004247:DNA / Q000235:genetics*; D006801:Humans; D007109:Immunity / Q000235:genetics*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015854:Up-Regulation / Q000235:genetics
D004247:DNA