Toward a bioartificial pancreas: diffusion of insulin and IgG across immunoprotective membranes with controlled hydrophilic channel diameters.
Research continued toward a bioartificial pancreas (BAP). Our BAPs consist of a perforated nitinol scaffold coated with reinforced amphiphilic conetwork membranes and contain live pancreatic islets. The membranes are assemblages of cocontinuous hydrophobic domains and hydrophilic channels whose diameters were varied by the MW of hydrophilic segments between crosslinks (M(c,HI) = 32, 44, and 74 kg x mol(-1)). We studied the diffusion rate of insulin, BSA, and IgG across the membrane of the BAP in the absence of islets. Membranes of M(c,HI) = 74 kg x mol(-1) showed rapid insulin and BSA transport and negligible IgG diffusion. BAPs containing approximately 300 mouse islets showed appropriate response upon glucose challenge in vitro. The BAP implanted into diabetic mice reduced hyperglycemia and maintained islet viability for at least 4 d.
Kang
Jungmee
J
Institute of Polymer Science, The University of Akron, Akron, Ohio 44325-3909, USA.
Erdodi
Gabor
G
Kennedy
Joseph P
JP
Chou
Hongshiue
H
Lu
Lina
L
Grundfest-Broniatowski
Sharon
S
D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D021522:Bioartificial Organs; D001786:Blood Glucose / Q000378:metabolism; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D004058:Diffusion; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis / Q000737:chemistry; D005947:Glucose / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000378:metabolism; D010179:Pancreas; D010539:Permeability; D011140:Polyurethanes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012833:Siloxanes / Q000737:chemistry
D000178:Acrylamides; D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D007328:Insulin; D008567:Membranes, Artificial; D011140:Polyurethanes; D012833:Siloxanes; C402373:poly(methylhydrosiloxane); D012710:Serum Albumin, Bovine; C099046:N,N-dimethylacrylamide; D005947:Glucose; D010100:Oxygen