Natural variation for seed dormancy in Arabidopsis is regulated by additive genetic and molecular pathways.
Timing of germination is presumably under strong natural selection as it determines the environmental conditions in which a plant germinates and initiates its postembryonic life cycle. To investigate how seed dormancy is controlled, quantitative trait loci (QTL) analyses has been performed in six Arabidopsis thaliana recombinant inbred line populations by analyzing them simultaneously using a mixed model QTL approach. The recombinant inbred line populations were derived from crosses between the reference accession Landsberg erecta (Ler) and accessions from different world regions. In total, 11 delay of germination (DOG) QTL have been identified, and nine of them have been confirmed by near isogenic lines (NILs). The absence of strong epistatic interactions between the different DOG loci suggests that they affect dormancy mainly by distinct genetic pathways. This was confirmed by analyzing the transcriptome of freshly harvested dry seeds of five different DOG NILs. All five DOG NILs showed discernible and different expression patterns compared with the expression of their genetic background Ler. The genes identified in the different DOG NILs represent largely different gene ontology profiles. It is proposed that natural variation for seed dormancy in Arabidopsis is mainly controlled by different additive genetic and molecular pathways rather than epistatic interactions, indicating the involvement of several independent pathways.
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands. l.bentsink@uu.nl
Leónie
L
Bentsink
Johannes
J
Hanson
Corrie J
CJ
Hanhart
Hetty
H
Blankestijn-de Vries
Colin
C
Coltrane
Paul
P
Keizer
Mohamed
M
El-Lithy
Carlos
C
Alonso-Blanco
M Teresa
MT
de Andrés
Matthieu
M
Reymond
Fred
F
van Eeuwijk
Sjef
S
Smeekens
Maarten
M
Koornneef
D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D014644:Genetic Variation; D040641:Quantitative Trait Loci; D012639:Seeds
D029681:Arabidopsis Proteins; C532384:DOG1 protein, Arabidopsis