Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients.
A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.
Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi 4701192, Japan. mihira@fujita-hu.ac.jp
Masaru
M
Ihira
Hiroko
H
Sugiyama
Yoshihiko
Y
Enomoto
Yuki
Y
Higashimoto
Ken
K
Sugata
Yoshizo
Y
Asano
Tetsushi
T
Yoshikawa
D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019349:Roseolovirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology*; D014180:Transplantation; D014773:Virology / Q000379:methods*
D017931:DNA Primers; D004279:DNA, Viral; C531960:IE2 protein, human herpesvirus 6A; D017874:Immediate-Early Proteins