Disposable pipette extraction for the analysis of pesticides in fruit and vegetables using gas chromatography/mass spectrometry.
Organochlorine, organophosphate pesticides and fungicides in fruits and vegetables were analyzed using disposable pipette extraction (DPX) followed by gas chromatography-mass spectrometry-selective ion monitoring (GC/MS-SIM). The intrinsic rapid mixing capabilities of DPX result in fast and efficient extractions, and eluates are concentrated by using minimal elution solvent volumes rather than solvent evaporation methods. Matrix-matched calibrations were performed with reversed phase mechanisms (DPX-RP), and the limits of detection (LOD) were determined to be lower than 0.1 microg/mL for all targeted pesticides in carrot and orange sample matrices. Coefficients of determination (r(2)) were greater than 0.995 for most studied pesticides. DPX-RP exhibited recoveries between 72 and 116% for nonpolar and slightly polar pesticides (logP>2) with most of the recoveries over 88%. Only very polar pesticides (e.g., acephate, mathamidophos) were not extracted well using DPX-RP.
Department of Chemistry & Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA.
Hongxia
H
Guan
William E
WE
Brewer
Sherry T
ST
Garris
Stephen L
SL
Morgan
D000327:Adsorption; D002138:Calibration; D056148:Chromatography, Reverse-Phase; D032084:Citrus sinensis / Q000737:chemistry*; D018552:Daucus carota / Q000737:chemistry*; D004006:Dichlorvos / Q000737:chemistry; D004209:Disposable Equipment; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D008799:Mevinphos / Q000737:chemistry; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results
D010575:Pesticides; D008799:Mevinphos; D004006:Dichlorvos