The effect of dopamine agonists: the expression of GDNF, NGF, and BDNF in cultured mouse astrocytes.
In Parkinson's disease, cell death is selectively induced in mesencephalic nigral dopaminergic neurons. At present, no disease modifying therapy or radical treatment has been found for this disease. Some dopamine agonists may have a neuroprotective action in cultured cells and animal models. In the present study, we examined stimulating effects of a non-ergoline D(2) dopamine agonist, ropinirole, on synthesis/secretion of neurotrophic factors, including NGF, BDNF, and GDNF, in cultured mouse astrocytes. These effects were compared with those of ergoline dopamine agonists, SKF-38393, a D(1) agonist, bromocriptine, D(2) agonist, and apomorphine, D(1)/D(2) agonist. Ropinirole elevated GDNF levels to 4-fold, and NGF levels to 6.3-fold, compared with the control group. Of the dopamine agonists examined, ropinirole produced and secreted more GDNF than a 1.8-fold greater amount of apomorphine, a lesser amount of bromocriptine, or a 2.8-fold greater amount of SKF-38393, which served as the control group.
Clinical Research Center, National Hospital Organization, Utano National Hospital, Kyoto 616-8255, Japan.
Kiyoe
K
Ohta
Sadako
S
Kuno
Seiji
S
Inoue
Erika
E
Ikeda
Aya
A
Fujinami
Mitsuhiro
M
Ohta
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D001971:Bromocriptine / Q000494:pharmacology; D002478:Cells, Cultured; D018491:Dopamine Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism*; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D020932:Nerve Growth Factor / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017447:Receptors, Dopamine D1 / Q000819:agonists; D017448:Receptors, Dopamine D2 / Q000819:agonists; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D018491:Dopamine Agonists; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007211:Indoles; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; C046649:ropinirole; D001971:Bromocriptine; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D020932:Nerve Growth Factor; D001058:Apomorphine