Somatic embryogenesis and vegetative cutting capacity are under distinct genetic control in Coffea canephora Pierre.
The purpose of the study was to evaluate the possible genetic effect on vegetative propagation of Coffea canephora. Diversity for somatic embryogenesis (SE) ability was observed not only among two groups of C. canephora Pierre (Congolese and Guinean), but also within these different genetic groups. The results therefore showed that, under given experimental conditions, SE ability is depending on genotype. Furthermore the detection of quantitative trait loci (QTLs) controlling the SE and cutting abilities of C. canephora was performed on a large number of clones including accessions from a core collection, three parental clones and their segregating progenies. On the one hand we detected eight QTLs determining SE. Six positive QTLs for SE ability, whatever the criteria used to quantify this ability, were localized on one single chromosome region of the consensus genetic map. Two negative QTLs for SE ability (frequency of micro calli without somatic embryo) were detected on another linkage group. Deep analysis of the six QTLs detected for SE ability came to the conclusion that they can be assimilated to one single QTL explaining 8.6-12.2% of the observed variation. On the other hand, two QTLs for average length of roots and length of the longest sprouts of cuttings were detected in two linkage groups. These QTLs detected for cutting ability are explaining 12-27% of the observed variation. These observations led to conclude that SE and cutting abilities of C. canephora Pierre appeared to be genetic dependent but through independent mechanisms.
Priyono
Indonesian Coffee and Cocoa Research Institute, Jl. PB. Sudirman 90, Jember 68118, Indonesia.
Florin
Bruno
B
Rigoreau
Michel
M
Ducos
Jean-Paul
JP
Sumirat
Ucu
U
Mawardi
Surip
S
Lambot
Charles
C
Broun
Pierre
P
Pétiard
Vincent
V
Wahyudi
Teguh
T
Crouzillat
Dominique
D
D002678:Chimera; D002874:Chromosome Mapping; D040503:Coffea / Q000196:embryology* / Q000235:genetics* / Q000254:growth & development; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D040641:Quantitative Trait Loci; D046509:Tissue Culture Techniques
D018744:DNA, Plant