Trichomonas vaginalis: dehydroepiandrosterone sulfate and 17beta-estradiol alter NTPDase activity and gene expression.
We investigated the effect of dehydroepiandrosterone sulfate (DHEAS) and 17beta-estradiol on NTPDase activity in fresh clinical (VP60) and long-term-grown (30236 ATCC) isolates of Trichomonas vaginalis followed by NTPDase gene transcriptional analysis. ATP hydrolysis was activated in vitro by 17beta-estradiol (0.01-1.0microM) in the VP60 isolate. Treatment for 2h with 17beta-estradiol (0.01-1microM) promoted an inhibition in nucleotide hydrolysis in the 30236 isolate whereas the 12h-treatment promoted an activation of nucleotide hydrolysis in both isolates. ADP hydrolysis was inhibited in vitro by 1.0-5.0microM DHEAS in the ATCC isolate. The treatment with DHEAS (0.01-1.0microM) for 2h inhibited ATP and ADP hydrolysis in VP60; however, during a 12h-treatment with DHEAS, nucleotide hydrolysis was inhibited in both isolates. Two NTPDase orthologous (NTPDaseA and NTPDaseB) were identified and the treatment with DHEAS for 12h was able to inhibit mRNA NTPDaseA transcript levels from the VP60. These findings demonstrate that NTPDase activity and gene expression pattern are modulated by exposure to steroids in T. vaginalis.
Rückert
Caroline
C
Programa de Pós-Graduação em Biologia Celular e Molecular, Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga 6681, Porto Alegre, RS, Brazil.
Stuepp
Cristiane dos Santos
Cdos S
Gottardi
Barbara
B
Rosa
Jessica
J
Cisilotto
Julia
J
Borges
Fernanda Pires
FP
Rosemberg
Denis Broock
DB
Bogo
Mauricio Reis
MR
Tasca
Tiana
T
De Carli
Geraldo Attilio
GA
Bonan
Carla Denise
CD
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D043583:Nucleoside-Triphosphatase / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D004967:Estrogens; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D043583:Nucleoside-Triphosphatase