A plant-tissue-based biophotonic method for herbicide sensing.
Herbicide application incurs water contamination and thus effective herbicide detection methods are needed. In this research, a kinetic model was developed to describe the processes of herbicide diffusion into plant tissues and binding to the active sites. DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea], a commonly used herbicide, was used as a test chemical and its diffusion into plant leaves and binding to plastoquinone B (Q(B)) sites were analyzed by using the model. The dependency of delayed fluorescence (DF) emission on unbound Q(B) sites was first derived theoretically and then observed experimentally. This yields a biophotonic method to measure the concentrations of DCMU and other herbicides that function by a similar mechanism. Experiments were performed to validate the developed method.
Guo
Ya
Y
Department of Biological Engineering, University of Missouri, Columbia, MO 65211, USA.
Tan
Jinglu
J
D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006540:Herbicides / Q000032:analysis* / Q000493:pharmacokinetics*; D008954:Models, Biological; D010783:Photometry / Q000295:instrumentation*; D017785:Photons; D018515:Plant Leaves / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006540:Herbicides