Quick preparation of genomic DNA from Drosophila.
This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).
Audrey M
AM
Huang
E Jay
EJ
Rehm
Gerald M
GM
Rubin
D000818:Animals; D004247:DNA / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005821:Genetic Techniques; D016678:Genome; D008967:Molecular Biology / Q000379:methods; D012313:RNA / Q000302:isolation & purification; D012260:Ribonucleases / Q000235:genetics*
D012313:RNA; D004247:DNA; D012260:Ribonucleases