Diminished lipocalin-type prostaglandin D(2) synthase expression in human lung tumors.
Previously, we demonstrated that lipocalin-type prostaglandin D(2) synthase (L-PGDS) induces apoptosis and prevents cell cycle progression in several cell types. In this study we determined the expression of L-PGDS in a variety of human lung tumor types. While L-PGDS expression was evident in the surrounding margins, we observed significantly decreased protein and gene expression in the tumor tissue. Using RT-PCR we demonstrated that L-PGDS gene expression decreased proportionately with tumor progression. In addition, we demonstrated that exogenously added L-PGDS could suppress the hyperproliferation and PDGF-stimulated migration of A549 cells, a cultured carcinomic human alveolar basal epithelial cell line. We conclude that L-PGDS may play a key role in modulating lung cancer growth and may offer a novel diagnostic and therapeutic approach for treatment.
Ragolia
Louis
L
Vascular Biology Institute, Winthrop-University Hospital, Mineola, NY 11501, USA. LRagolia@Winthrop.org
Palaia
Thomas
T
Hall
Christopher E
CE
Klein
Jonathan
J
Büyük
Arzu
A
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D018450:Disease Progression; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology; D054834:Lipocalins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054834:Lipocalins; D010982:Platelet-Derived Growth Factor; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase