Administration of antibiotics during infancy promoted the development of atopic dermatitis-like skin lesions in NC/Nga mice.
This study aimed to determine whether oral antibiotic administration during infancy is associated with the spontaneous development of atopic dermatitis-like skin lesions by modulating intestinal microbiota. Female NC/Nga mice at 3 weeks of age were orally administered kanamycin or polymyxin B. Clinical symptoms, scratching behavior, and serum antibody levels were evaluated. Changes in intestinal microbiota were determined by culture-independent analysis and cultural analysis. The kanamycin-treated mice showed higher clinical scores and scratching frequency than the control mice. IgE levels were significantly higher in the kanamycin-treated mice than in the control mice. Transient changes in intestinal microbiota were observed under kanamycin treatment. Polymyxin B treatment failed to affect scratching behavior. These results suggest that oral administration of kanamycin during infancy promoted the development of atopic dermatitis-like skin lesions in NC/Nga mice and was associated with a transient change in intestinal microbiota.
Creative Research Initiative Sousei, Hokkaido University, Sapporo, Japan. nabej@affrc.go.jp
Jun
J
Watanabe
Reiko
R
Fujiwara
Naho
N
Sasajima
Susumu
S
Ito
Kei
K
Sonoyama
D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects*; D003876:Dermatitis, Atopic / Q000473:pathology*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D011537:Pruritus / Q000473:pathology; D012720:Severity of Illness Index; D012867:Skin / Q000473:pathology*
D000900:Anti-Bacterial Agents; D007073:Immunoglobulin E