A bio-friendly and economical technique for chronic implantation of multiple microelectrode arrays.
Many neurophysiological experiments on rodents and non-human primates involve the implantation of more than one multi-electrode array to record from many regions of the brain. So called 'floating' microelectrode arrays are implanted in cortical regions of interest and are coupled via a flexible cable to their connectors which are fixed to the skull by a cement cap or a titanium pedestal, such as the Cereport system, which has been approved for human use. The use of bone cement has several disadvantages including the creation of infection prone areas at the interface with the skull and surrounding skin. Alternatively, the more biocompatible Cereport has a limited carrying capacity and is far more expensive. In this paper, we describe a new implantation technique, which combines the biocompatibility of titanium, a high carrying capacity with a minimal skull footprint, and a decreased chance of infection, all in a relatively inexpensive package. This technique utilizes an in-house fabricated 'Nesting Platform' (NP), mounted on a titanium headpost to hold multiple connectors above the skin, making the headpost the only transcutaneous object. The use of delrin, a durable, lightweight and easily machinable material, allows easy customization of the NP for a wide variety of floating electrodes and their connectors. The ultimate result is a longer survival time with superior neural recordings that can potentially last longer than with traditional implantation techniques.
Department of Physiology and Pharmacology, SUNY Downstate Medical Center, Brooklyn, 450 Clarkson Av, Box# 31, Brooklyn, NY 11203, USA.
Pratik Y
PY
Chhatbar
Lee M
LM
von Kraus
Mulugeta
M
Semework
Joseph T
JT
Francis
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000502:physiology; D020099:Coated Materials, Biocompatible; D004582:Electronics, Medical / Q000295:instrumentation / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005260:Female; D008255:Macaca radiata; D008839:Microelectrodes / Q000639:trends; D023421:Models, Animal; D009474:Neurons / Q000502:physiology; D009482:Neurophysiology / Q000295:instrumentation* / Q000379:methods; D019635:Neurosurgical Procedures / Q000295:instrumentation* / Q000379:methods; D019919:Prosthesis Implantation / Q000379:methods*; D012117:Resins, Synthetic; D014025:Titanium
D020099:Coated Materials, Biocompatible; D012117:Resins, Synthetic; C010102:delrin; D014025:Titanium