Analysis of MMP-dependent cell migration and invasion.
Analysing cell migration and invasion is of interest to many investigators as they mimic a part of physiological or pathological events. In this chapter, methods to analyse MMP-dependent 2D and 3D cell migration are described in detail. To study 2D cell migration, the phagokinetic track motility assay can be used. To study 3D cell migration, Matrigel invasion assay or microcarrier beads invasion assay can be used to obtain quantitative results.
Imperial College London, Kennedy Institute of Rheumatology, London, UK.
Ralf
R
Palmisano
Yoshifumi
Y
Itoh
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D007797:Laminin / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008863:Microspheres; D011509:Proteoglycans / Q000378:metabolism
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D020782:Matrix Metalloproteinases