Gene expression profiles of diabetic mice treated with whole body hyperthermia: A high-density DNA microarray analysis.
Previously we have demonstrated that whole body hyperthermia (WBH) improves insulin resistance in diabetic mice. The aim of the present study was to perform a gene expression analysis of the liver and adipose tissue of obesity-induced insulin resistant diabetic mice (db/db mice) after WBH and to define the molecules that play the important role in improvement of insulin resistance by WBH. Male db/db mice were treated with WBH 3 times per week for 12 weeks. Total RNA was extracted from the liver and adipose tissue of db/db mice, and differences in the gene expression profiles among db/+ mice, untreated db/db mice, and WBH-treated db/db mice were investigated using a high-density DNA microarray. WBH directly targets liver and adipose tissue, resulting in modifications in NF-kappaB and IL-6 signalling pathways, as well as lipid metabolism. Although the mechanisms have not yet been completely investigated, we can conclude that WBH may provide a new therapeutic or preventive modality against type 2 diabetes mellitus and metabolic or insulin resistance syndrome through the modification of several signalling pathways.
Kyoto Prefectural University of Medicine, Japan.
Satoshi
S
Kokura
Satoko
S
Adachi
Katsura
K
Mizushima
Tetsuya
T
Okayama
Takeshi
T
Hattori
Toshimitsu
T
Okuda
Nami
N
Nakabe
Emiko
E
Manabe
Takeshi
T
Ishikawa
Osamu
O
Handa
Tomohisa
T
Takagi
Yuji
Y
Naito
Toshikazu
T
Yoshikawa
D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D016000:Cluster Analysis; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006979:Hyperthermia, Induced; D007333:Insulin Resistance / Q000235:genetics; D008099:Liver / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction / Q000502:physiology