CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics.
Owing to its availability, ease of collection, and correlation with pathophysiology of diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited clinical impact, due to factors such as modest numbers of subjects, absence of disease controls, small numbers of defined biomarkers, and diversity of analytical platforms. Therefore, it is difficult to merge biomarkers from different studies into a broadly applicable human urinary proteome database. Ideally, the methodology for defining the biomarkers should combine a reasonable analysis time with high resolution, thereby enabling the profiling of adequate samples and recognition of sufficient features to yield robust diagnostic panels. Capillary electrophoresis coupled to mass spectrometry (CE-MS), which was used to analyze urine samples from healthy subjects and patients with various diseases, is a suitable approach for this task. The database of these datasets compiled from the urinary peptides enabled the diagnosis, classification, and monitoring of a wide range of diseases. CE-MS exhibits excellent performance for biomarker discovery and allows subsequent biomarker sequencing independent of the separation platform. This approach may elucidate the pathogenesis of many diseases, and better define especially renal and urological disorders at the molecular level.
Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Joshua J
JJ
Coon
Petra
P
Zürbig
Mohammed
M
Dakna
Anna F
AF
Dominiczak
Stéphane
S
Decramer
Danilo
D
Fliser
Moritz
M
Frommberger
Igor
I
Golovko
David M
DM
Good
Stefan
S
Herget-Rosenthal
Joachim
J
Jankowski
Bruce A
BA
Julian
Markus
M
Kellmann
Walter
W
Kolch
Ziad
Z
Massy
Jan
J
Novak
Kasper
K
Rossing
Joost P
JP
Schanstra
Eric
E
Schiffer
Dan
D
Theodorescu
Raymond
R
Vanholder
Eva M
EM
Weissinger
Harald
H
Mischak
Philippe
P
Schmitt-Kopplin