The behaviour of neural stem cells on polyhydroxyalkanoate nanofiber scaffolds.
Polyhydroxyalkanoates (PHA) have demonstrated their potentials as medical implant biomaterials. Neural stem cells (NSCs) grown on/in PHA scaffolds may be useful for repairing central nervous system (CNS) injury. To investigate this possibility, nanofiber matrices (scaffolds) prepared from several PHA via a novel phase separation process were studied to mimic natural extracellular matrix (ECM), and rat-derived NSCs grown in the PHA matrices were characterized regarding their in vitro differentiation behaviors. All three PHA materials including poly(3-hydroxybutyrate) (PHB), copolymer of 3-hydroxybutyrate and 4-hydroxybutyrate (P3HB4HB), and copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) supported NSC growth and differentiation both on their 2D films and 3D matrices. Among three PHA nanofiber matrices, PHBHHx one showed the strongest potentials to promote NSC differentiation into neurons which is beneficial for CNS repair. Compared to the 2D films, 3D nanofiber matrices appeared to be more suitable for NSC attachment, synaptic outgrowth and synaptogenesis. It was suggested that PHBHHx nanofiber scaffolds (matrices) that promote NSC growth and differentiation, can be developed for treating central nervous system injury.
Xu
Xian-Yi
XY
Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, China.
Li
Xiao-Tao
XT
Peng
Si-Wu
SW
Xiao
Jian-Feng
JF
Liu
Chao
C
Fang
Guo
G
Chen
Kevin C
KC
Chen
Guo-Qiang
GQ
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D019344:Lactic Acid / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D011091:Polyesters; D054813:Polyhydroxyalkanoates / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D000091402:Prohibitins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*; D014404:Tubulin / Q000378:metabolism
C000717332:Phb protein, rat; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011108:Polymers; D000091402:Prohibitins; D014404:Tubulin; D019344:Lactic Acid; C033616:poly(lactide)