Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum.
The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15(500) (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density.
Fujiwara
Masashi
M
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo 060-8628, Japan.
Aizu
Yu
Y
Shioya
Itaru
I
Takagi
Mutsumi
M
D000818:Animals; D016466:CHO Cells; D002417:Cattle / Q000097:blood*; D018929:Cell Culture Techniques / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005399:Fishes / Q000097:blood*; D044967:Serum / Q000378:metabolism*
D016895:Culture Media, Serum-Free