A convenient method for saponin isolation in tumour therapy.
Saponinum album (Merck), which is a crude mixture of saponins from Gypsophila paniculata L., was shown to improve the anti cancer therapy when used in vivo in combination with saporin-based targeted toxins. Unfortunately saponinum album cannot be used for further development since Merck has ceased its production in the 1990s. As pure saponins are mandatory for use in medical purposes we developed a convenient method for saponin isolation directly from the roots of Gypsophila paniculata L. The developed method is rapid, cheap and scaling up is also possible. By combining dialysis and HPLC three saponins were isolated in a one-step procedure. Chemical structures of the purified saponins were characterized by extensive one and two-dimensional NMR-spectroscopy and by using ESI-TOF-MS. The biological activities of the purified saponins were also investigated. The method presented herein enabled a rapid and cheap isolation of saponins for tumour therapy.
Institute of Pharmacy, Free University Berlin, Königin-Luise-Str. 2+4, D-14195 Berlin, Germany.
Alexander
A
Weng
Kristina
K
Jenett-Siems
Peter
P
Schmieder
Diana
D
Bachran
Christopher
C
Bachran
Cornelia
C
Görick
Mayank
M
Thakur
Hendrik
H
Fuchs
Matthias F
MF
Melzig
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D029748:Caryophyllaceae / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins