Mechanism of neurodegeneration of neurons with mitochondrial DNA mutations.
Mutations of mitochondrial DNA are associated with a wide spectrum of disorders, primarily affecting the central nervous system and muscle function. The specific consequences of mitochondrial DNA mutations for neuronal pathophysiology are not understood. In order to explore the impact of mitochondrial mutations on neuronal biochemistry and physiology, we have used fluorescence imaging techniques to examine changes in mitochondrial function in neurons differentiated from mouse embryonic stem-cell cybrids containing mitochondrial DNA polymorphic variants or mutations. Surprisingly, in neurons carrying a severe mutation in respiratory complex I (<10% residual complex I activity) the mitochondrial membrane potential was significantly increased, but collapsed in response to oligomycin, suggesting that the mitochondrial membrane potential was maintained by the F(1)F(o) ATPase operating in 'reverse' mode. In cells with a mutation in complex IV causing approximately 40% residual complex IV activity, the mitochondrial membrane potential was not significantly different from controls. The rate of generation of mitochondrial reactive oxygen species, measured using hydroethidium and signals from the mitochondrially targeted hydroethidine, was increased in neurons with both the complex I and complex IV mutations. Glutathione was depleted, suggesting significant oxidative stress in neurons with a complex I deficiency, but not in those with a complex IV defect. In the neurons with complex I deficiency but not the complex IV defect, neuronal death was increased and was attenuated by reactive oxygen species scavengers. Thus, in neurons with a severe mutation of complex I, the maintenance of a high potential by F(1)F(o) ATPase activity combined with an impaired respiratory chain causes oxidative stress which promotes cell death.
Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London, WC1N 3BG, UK. a.abramov@ucl.ac.uk
Andrey Y
AY
Abramov
Tora K
TK
Smulders-Srinivasan
Denise M
DM
Kirby
Rebeca
R
Acin-Perez
José Antonio
JA
Enriquez
Robert N
RN
Lightowlers
Michael R
MR
Duchen
Douglass M
DM
Turnbull
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D004272:DNA, Mitochondrial; D042967:Electron Transport Complex I / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione / Q000172:deficiency / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D009154:Mutation; D009410:Nerve Degeneration / Q000235:genetics* / Q000503:physiopathology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
D004272:DNA, Mitochondrial; D004791:Enzyme Inhibitors; D009840:Oligomycins; D017382:Reactive Oxygen Species; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D005978:Glutathione; D002118:Calcium