Fixation of Ilyanassa snail embryos and larvae.
The marine gastropod Ilyanassa obsoleta is a long-standing and very useful model for studies of embryonic development. It is an especially important model for spiralian development, and for studies of asymmetric cell division. The embryos are amenable to classic embryological manipulation techniques, as well as a growing number of molecular approaches. Ilyanassa is also an important model for studies of metamorphosis, the ecology of parasitism, the effects of environmental contaminants on morphology and sexual function, and comparative neurobiology. Ilyanassa embryos are particularly well suited for RNA and protein localization studies because of the relatively large cells and favorable properties for imaging. This protocol describes how to fix and store Ilyanassa embryos and larvae for use in whole-mount in situ hybridization and immunohistochemical studies.
Gharbiah
Maey
M
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA.
Cooley
James
J
Leise
Esther M
EM
Nakamoto
Ayaki
A
Rabinowitz
Jeremy S
JS
Lambert
J David
JD
Nagy
Lisa M
LM
D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000196:embryology*; D005821:Genetic Techniques; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007814:Larva / Q000235:genetics* / Q000254:growth & development; D008675:Metamorphosis, Biological; D012313:RNA / Q000378:metabolism; D012908:Snails / Q000196:embryology* / Q000502:physiology*
D012313:RNA