Protection of burn-induced skin injuries by the flavonoid kaempferol.
Thermal burn injury induces inflammatory cell infiltrates in the dermis and thickening of the epidermis. Following a burn injury, various mediators, including reactive oxygen species (ROS), are produced in macrophages and neutrophils, exposing all tissues to oxidative injury. The anti-oxidant activities of flavonoids have been widely exploited to scavenge ROS. In this study, we observed that several flavonoids-kaempferol, quercetin, fisetin, and chrysin-inhibit LPS-induced IL-8 promoter activation in RAW 264.7 cells. In contrast with quercetin and fisetin, pretreatment of kaempferol and chrysin did not decrease cell viability. Inflammatory cell infiltrates in the dermis and thickening of the epidermis induced by burn injuries in mice was relieved by kaempferol treatment. However, the injury was worsened by fisetin, quercetin, and chrysin. Expression of TNF-a induced by burn injuries was decreased by kaempferol. These findings suggest the potential use of kaempferol as a therapeutic in thermal burn-induced skin injuries. [BMB reports 2010; 43(1): 46-51].
Park
Byoung Kwon
BK
Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
Lee
Soohyoung
S
Seo
Jae-Nam
JN
Rhee
Jae Won
JW
Park
Jae-Bong
JB
Kim
Yong-Sun
YS
Choi
Ihn-Geun
IG
Kim
Young-Eun
YE
Lee
Younghee
Y
Kwon
Hyung-Joo
HJ
D000818:Animals; D002056:Burns / Q000188:drug therapy*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000494:pharmacology; D044948:Flavonols; D016209:Interleukin-8 / Q000378:metabolism; D044949:Kaempferols / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D011794:Quercetin / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D005419:Flavonoids; D044948:Flavonols; D016209:Interleukin-8; D044949:Kaempferols; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C043561:chrysin; C006552:kaempferol; D011794:Quercetin; C017875:fisetin