Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate.
The preparation of 1-deoxy-d-xylulose 5-phosphate, the key intermediate of MEP biosynthetic pathway for terpenoids by using recombinant 1-deoxy-d-xylulose 5-phosphate synthase of Rhodobacter capsulatus was optimized. The simple one-pot synthesis coupling with a newly established ion-exchange purification process affords the target compound with more than 80% yield and high purity (>95%). The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials.
National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi'an, Shaanxi, PR China.
Yue-Fei
YF
Zhou
Zhe
Z
Cui
Heng
H
Li
Jie
J
Tian
Wen-Yun
WY
Gao
D006863:Hydrogen-Ion Concentration; D010428:Pentosephosphates / Q000096:biosynthesis* / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000201:enzymology; D013696:Temperature; D014166:Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
C109460:1-deoxylulose 5-phosphate; D010428:Pentosephosphates; D011994:Recombinant Proteins; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase