Clinical performance of a handheld digital infrared monocular pupillometer for measurement of the dark-adapted pupil diameter.
OBJECTIVE
To compare the accuracy of a handheld infrared digital pupillometer and digital infrared photography for measurement of the dark-adapted pupil diameter.


METHODS
Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.


METHODS
The right horizontal pupil diameter in healthy volunteers was measured using a NeurOptics PLR-200 pupillometer and then videographed using the infrared function of a CyberShot video camera after 2 minutes and 5 minutes dark adaptation at 1 lux ambient illumination. The best still image was extracted from the video file, and the horizontal pupil diameter was determined by comparison against an internal photographic length standard using digital image software. Accommodation and alertness were controlled during testing.


RESULTS
The mean horizontal pupil diameter by infrared photography after 2 minutes of dark adaptation by subject age was 7.71 mm for ages 20 to 29 years, 6.80 mm for ages 30 to 39 years, 6.53 mm for ages 40 to 49 years, 5.94 mm for ages 50 to 59 years, and 6.01 mm for ages 60 to 69 years. The mean difference (infrared photography minus pupillometer) was +0.09 mm (range +0.30 to -0.14 mm) at 2 minutes of adaptation and +0.07 mm (range +0.25 to -0.13 mm) at 5 minutes.


CONCLUSIONS
The pupillometer accurately measured the horizontal pupil diameter at 1 lux, with no measurement more than 0.3 mm different from infrared photography measurements. The pupillometer had a slight negative bias that is unlikely to introduce an error greater than 0.5 mm in clinical measurements.
Bradley
Jay C
JC
Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Bentley
Karl C
KC
Mughal
Aleem I
AI
Brown
Sandra M
SM
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology; D003623:Dark Adaptation / Q000502:physiology*; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation*; D005260:Female; D006801:Humans; D007259:Infrared Rays; D008297:Male; D008875:Middle Aged; D010781:Photography; D011680:Pupil / Q000502:physiology*; D015203:Reproducibility of Results; D055815:Young Adult