Electrografted poly(N-mercaptoethyl acrylamide) and Au nanoparticles-based organic/inorganic film: a platform for the high-performance electrochemical biosensors.
In this study, we describe the use of the combination of eletrografting poly(N-mercaptoethyl acrylamide) and Au nanoparticles in the construction of high-performance biosensors. The poly(N-mercaptoethyl acrylamide) was electrografted onto the glassy carbon electrode surface, which provided a strongly adhering primer film for the stable attachment of Au nanoparticles and horseradish peroxidase (HRP) enzymes. The performances of the biosensors based on the HRP immobilized in the Au/poly(N-mercaptoethyl acrylamide) composite film were investigated. A couple of redox peaks were obtained, indicating that the Au nanoparticles could facilitate the direct-electron transfer between HRP and the underlying electrode. The biosensor showed an excellent electrocatalytic activity toward the reduction of hydrogen oxide and long-term stability, owing to the stable electrografted film and biocompatible Au nanoparticles. Our results demonstrate that the combination of electrografting and Au nanoparticles provides a promising platform for the immobilization of biomolecules and analysis of redox enzymes for their sensing applications.
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Limiao
L
Li
Shoujiang
S
Xu
Zhifeng
Z
Du
Yanfang
Y
Gao
Jinghong
J
Li
Taihong
T
Wang
D000180:Acrylic Resins / Q000737:chemistry*; D015374:Biosensing Techniques; D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D011108:Polymers; C550609:poly(N-mercaptoethyl acrylamide); D006046:Gold; D006735:Horseradish Peroxidase