MR molecular imaging of HER-2 in a murine tumor xenograft by SPIO labeling of anti-HER-2 affibody.
In vivo molecular imaging is a rapidly growing research area both for basic and clinical science. Non-invasive imaging of in vivo conditions at the molecular level increases understanding of the biological characteristics of normal and diseased tissues without the need for invasive surgical procedures. Among the various imaging modalities, magnetic resonance imaging (MRI) has garnered interest as a molecular imaging modality due to its high spatial resolution. Here, we have demonstrated that the combined use of HER-2 targeting affibody, a small 7 kDa molecule that behaves similarly to antibodies, and superparamagnetic iron oxide (SPIO) can non-invasively image HER-2 expressing cells or tissues both in vitro and in vivo by MRI. This preliminary study demonstrates that affibody-SPIO is a feasible, target-specific contrast agent for in vivo MR molecular imaging.
Kinoshita
Manabu
M
Department of Neurosurgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. m-kinoshita@nsurg.med.osaka-u.ac.jp
Yoshioka
Yoshichika
Y
Okita
Yoshiko
Y
Hashimoto
Naoya
N
Yoshimine
Toshiki
T
D000818:Animals; D045744:Cell Line, Tumor; D054815:Cyclic Nucleotide-Gated Cation Channels; D003911:Dextrans; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D006801:Humans; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013194:Staining and Labeling; D023041:Xenograft Model Antitumor Assays
D054815:Cyclic Nucleotide-Gated Cation Channels; D003911:Dextrans; D052203:Ferrosoferric Oxide; D007501:Iron; D058185:Magnetite Nanoparticles; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C508053:ERBB2 protein, human; C065507:ferumoxides