Determination of catechin diastereomers from the leaves of Byrsonima species using chiral HPLC-PAD-CD.
When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase.
Institute of Chemistry, São Paulo State University, Unesp, Francisco Degni Street, Araraquara-SP, Brazil.
Daniel
D
Rinaldo
João M
JM
Batista
Juliana
J
Rodrigues
Ana C
AC
Benfatti
Clenilson M
CM
Rodrigues
Lourdes C
LC
dos Santos
Maysa
M
Furlan
Wagner
W
Vilegas
D002392:Catechin / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D004566:Electrodes; D016014:Linear Models; D029581:Malpighiaceae / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism
D002392:Catechin; D000432:Methanol