Inhibition of tomato yellow leaf curl virus (TYLCV) using whey proteins.
The antiviral activity of native and esterified whey proteins fractions (alpha-lactalbumin, beta-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (TYLCV) on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using alpha-lactoferrin and alpha-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native beta-lactoglobulin showed inhibition after 7 and 15 days whereas esterified beta-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified alpha-lactalbumin whereas native alpha-lactalbumin inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants.
Abdelbacki
Ashraf M
AM
Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. amaeg@hotmail.com
Taha
Soad H
SH
Sitohy
Mahmoud Z
MZ
Abou Dawood
Abdelgawad I
AI
Abd-El Hamid
Mahmoud M
MM
Rezk
Adel A
AA
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D053839:Begomovirus / Q000187:drug effects*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D004279:DNA, Viral / Q000302:isolation & purification; D018551:Solanum lycopersicum / Q000821:virology*; D008894:Milk Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology*; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*; D000067816:Whey Proteins
D000998:Antiviral Agents; D001688:Biological Products; D004279:DNA, Viral; D008894:Milk Proteins; D000067816:Whey Proteins