Extraction of RNA from the plant Kalanchoë daigremontiana.
This protocol describes how to isolate total RNA from several tissues of Kalanchoë daigremontiana. Total RNA can be isolated by using the TRI-reagent method, scaled up for processing 2 g of tissue, or by using the protocol described here, which gives higher concentrations of high quality RNA. The resulting RNA can be used for various applications including generation of cDNA for reverse transcriptase-PCR (RT-PCR), Northern blots, or other purposes.
Garcês
Helena
H
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Sinha
Neelima
N
D001901:Botany / Q000379:methods*; D018076:DNA, Complementary / Q000378:metabolism; D017343:Genes, Plant; D005821:Genetic Techniques; D031962:Kalanchoe / Q000235:genetics*; D012313:RNA / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D018749:RNA, Plant; D012313:RNA