Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells.
Although adipose tissue is an expandable and readily attainable source of proliferating, multipotent stem cells, its potential for use in regenerative medicine has not been extensively explored. Here we report that adult human and mouse adipose-derived stem cells can be reprogrammed to induced pluripotent stem (iPS) cells with substantially higher efficiencies than those reported for human and mouse fibroblasts. Unexpectedly, both human and mouse iPS cells can be obtained in feeder-free conditions. We discovered that adipose-derived stem cells intrinsically express high levels of pluripotency factors such as basic FGF, TGFbeta, fibronectin, and vitronectin and can serve as feeders for both autologous and heterologous pluripotent cells. These results demonstrate a great potential for adipose-derived cells in regenerative therapeutics and as a model for studying the molecular mechanisms of feeder-free iPS generation and maintenance.
Sugii
Shigeki
S
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Kida
Yasuyuki
Y
Kawamura
Teruhisa
T
Suzuki
Jotaro
J
Vassena
Rita
R
Yin
Yun-Qiang
YQ
Lutz
Margaret K
MK
Berggren
W Travis
WT
Izpisúa Belmonte
Juan Carlos
JC
Evans
Ronald M
RM
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002678:Chimera; D018920:Coculture Techniques; D005346:Fibroblast Growth Factors; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005353:Fibronectins / Q000096:biosynthesis; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis; D019096:Vitronectin / Q000096:biosynthesis
D005353:Fibronectins; D016211:Transforming Growth Factor alpha; D019096:Vitronectin; D005346:Fibroblast Growth Factors