A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera.
A simple and sensitive high performance liquid chromatography method with photodiode array detection (HPLC-DAD) was developed for simultaneous determination of eight bioactive constituents (schisandrin, schisandrol B, schisantherin A, schisanhenol, anwulignan, deoxyshisandrin, schisandrin B and schisandrin C) in the ripe fruit of Schisandra sphenanthera and its traditional Chinese herbal preparations Wuzhi-capsule by optimizing the extraction, separation and analytical conditions of HPLC-DAD. The chemical fingerprint of S. sphenanthera was established using raw materials of 15 different origins in China. The chromatographic separations were obtained by an Agilent Eclipse XDB-C18 reserved-phase column (250 mm x 4.6 mm i.d., 5 microm) using gradient elution with water-formic acid (100:0.1, v/v) and acetonitrile, at a flow rate of 1.0 mL min(-1), an operating temperature of 35 degrees C, and a wavelength of 230 nm. The constituents were confirmed by (+) electrospray ionization LC-MS. The new method was validated and was successfully applied to simultaneous determination of components in 13 batches of Wuzhi-capsule. The results indicate that this multi-component determination method in combination with chromatographic fingerprint analysis is suitable for quantitative analysis and quality control of S. sphenanthera.
Wei
Hua
H
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Sun
Lianna
L
Tai
Zongguang
Z
Gao
Shouhong
S
Xu
Wen
W
Chen
Wansheng
W
D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000032:analysis / Q000737:chemistry; D004149:Dioxoles / Q000032:analysis / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis*; D011083:Polycyclic Compounds / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D032141:Schisandra / Q000737:chemistry*
D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; C015499:schizandrin B; C045309:schisanhenol; C034557:schizandrer A; C031409:schizandrin C; C011105:schizandrin