Differential protein levels and post-translational modifications in spinal cord injury of the rat.
Although changes in protein expression in spinal cord injury (SCI) would be of pivotal interest, information so far is limited. It was therefore the aim of the study to determine protein levels and post-translational modifications in the early phase following SCI in the rat. SCI was induced in Sprague-Dawley rats and sham operated rats served as controls. A gel-based proteomic approach using two-dimensional gel electrophoresis followed by quantification with specific software and subsequent identification of differentially expressed proteins by nano-ESI-LC-MS/MS was applied. Proteins of several pathways and cascades were dysregulated in SCI: 14-3-3 epsilon protein, dynein light chain 1, and tubulin beta-5 chain showed higher levels in SCI, whereas adenylyl cyclase associated protein 1, dihydropyrimidinase-related protein 2, F-actin capping protein subunit beta, glyceraldehyde-3-phosphate dehydrogenase, stress-induced phosphoprotein 1 and transthyretin showed lower levels in the injured tissue. Post-translational modifications indicated free oxygen radical attack on proteins in SCI. The occurrence of stress is indicated by deranged stress-induced phosphoprotein 1 and signaling abnormalities are reflected by adenylyl cyclase-associated protein 1 and 14-3-3 epsilon protein. The findings propose the involvement of the corresponding cascades and challenge further work into aberrant signaling and oxidative stress in SCI, which may form the basis for experimental intervention for spinal cord trauma.
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Leila
L
Afjehi-Sadat
Mika
M
Brejnikow
Sung Ung
SU
Kang
Vinay
V
Vishwanath
Nadja
N
Walder
Kurt
K
Herkner
Heinz
H
Redl
Gert
G
Lubec
D048948:14-3-3 Proteins / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D013058:Mass Spectrometry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014404:Tubulin / Q000032:analysis / Q000378:metabolism
D048948:14-3-3 Proteins; C081782:Cap1 protein, rat; D003598:Cytoskeletal Proteins; D011506:Proteins; D014404:Tubulin