The use of high-hydrostatic pressure treatment to decellularize blood vessels.
A decellularization method using high-hydrostatic pressure (HHP) technology (>600MPa) is described. The HHP disrupts the cells inside the tissue. The cell debris can be eliminated with a simple washing process, producing clean, decellularized tissue. In this study, porcine aortic blood vessel was decellularized by HHP. The mechanical properties and in vivo performance of the decellularized tissue were evaluated. Mechanical properties of the decellularized tissue were not altered by the HHP treatment. Reduced inflammation of the decellularized tissue was confirmed by xenogenic transplant experimentation. An allogenic transplantation study showed that decellularized blood vessel endured the arterial blood pressure, and there was no clot formation on the luminal surface. In addition, cellular infiltration into the vessel wall was observed 4 weeks after implantation, suggesting that HHP treatments could be applied widely as a high-quality decellularization method.
Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Seiichi
S
Funamoto
Kwangwoo
K
Nam
Tsuyoshi
T
Kimura
Ayako
A
Murakoshi
Yoshihide
Y
Hashimoto
Kazuo
K
Niwaya
Soichiro
S
Kitamura
Toshiya
T
Fujisato
Akio
A
Kishida
D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000502:physiology; D006874:Hydrostatic Pressure; D007053:Ice; D013552:Swine; D014183:Transplantation, Heterologous
D007053:Ice