[Effect of microwave radiation on primary cultured Sertoli cells].
OBJECTIVE
To explore whether microwave radiation may cause injury of primary cultured Sertoli cells.


METHODS
The model of primary cultured Sertoli cells in vitro was established, which was radiated by microwave with average power density 0, 30 and 100 mW/cm(2) for five minutes. The changes of cell cycle, apoptosis and death, and intracellular Ca2+ concentration in the Sertoli cells were measured at sixth hours through Annexin V-PI double labeling and Fluo-3-AM labeling, flow cytometry combined with laser scanning confocal microscopy after microwave exposure.


RESULTS
The numbers of Sertoli cells were obviously reduced in G0-G1 and G2-M phase (62.57% +/- 3.22% and 8.25% +/- 1.75%) and increased in S phase (29.17% +/- 4.87%) compared with the control groups (79.18% +/- 0.24%, 11.17% +/- 0.50% and 9.64% +/- 0.62%) (P < 0.05 or P < 0.01), but the changes of rate of apoptosis and death and intracellular Ca2+ concentration showed no difference at 6 h after exposure to 30 mW/cm(2) microwave. There was a significant increase in the Sertoli cell counts of G0-G1 phase (87.69% +/- 1.32%), and decrease in the Sertoli cell counts of G2-M and S phase (7.41% +/- 0.60% and 4.87% +/- 0.91%) (P < 0.01). There was also a significant increase in intracellular Ca2+ concentration and rate of apoptosis and death (P < 0.05 or P < 0.01) at 6 h after exposure to 100 mW/cm(2) microwave.


CONCLUSIONS
100 mW/cm(2) microwave radiation may cause growth inhibition and increase of apoptosis and death in the primary cultured Sertoli cells. The increase of intracellular Ca2+ concentration is one of the injury mechanisms.
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Xiao-fang
XF
Gao
Shui-ming
SM
Wang
Rui-yun
RY
Peng
Li-feng
LF
Wang
Hong-yan
HY
Zuo
Ya-bing
YB
Gao
Qi
Q
Dong
Bo
B
Dong
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000528:radiation effects; D002478:Cells, Cultured; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*
D002118:Calcium