Increase in membrane thickness during development compensates for eggshell thinning due to calcium uptake by the embryo in falcons.
We compared membrane thickness of fully developed eggs with those of non-developed eggs in different endangered falcon taxa. To our knowledge, membrane thickness variation during development has never been examined before in falcons or any other wild bird. Yet, the egg membrane constitutes an important protective barrier for the developing embryo. Because eggshell thinning is a general process that occurs during bird development, caused by calcium uptake by the embryo, eggs are expected to be less protected and vulnerable to breakage near the end of development. Thus, egg membranes could play an important protective role in the later stages of development by getting relatively thicker. We used linear mixed models to explore the variation in membrane thickness (n = 378 eggs) in relation to developmental stage, taxon, female age, mass and identity (73 females), egg-laying sequence (105 clutches) and the study zone. Our results are consistent with the prediction that egg membranes are thicker in fully developed eggs than in non-developed eggs, suggesting that the increase in membrane thickness during development may compensate for eggshell thinning. In addition, our data shown that thicker membranes are associated with larger, heavier and relatively wider eggs, as well as with eggs that had thinner eggshells. Egg-laying sequence, female age and the study zone did not explain the observed variation of membrane thickness in the falcon taxa studied. As we provide quantitative data on membrane thickness variation during development in falcons not subjected to contamination or food limitation (i.e. bred under captive conditions), our data may be used as a reference for studies on eggs from natural populations. Considering the large variation in membrane thickness and the multiple factors affecting on it and its importance in the protection of the embryo, we encourage other researchers to include measurements on membranes in studies exploring eggshell thickness variation.
Castilla
Aurora M
AM
Estación Biológica y de Cultura Científica de Sanaüja, Ap. Correos no 35, 25280 Solsona, Lleida, Spain. castilla.aurora@gmail.com
Van Dongen
Stefan
S
Herrel
Anthony
A
Francesch
Amadeu
A
Martínez de Aragón
Juan
J
Malone
Jim
J
Negro
Juan José
JJ
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002136:Calcium, Dietary; D004528:Egg Shell / Q000033:anatomy & histology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology*; D004785:Environmental Pollutants / Q000633:toxicity; D046093:Falconiformes / Q000254:growth & development / Q000502:physiology*; D005260:Female; D009425:Nesting Behavior / Q000187:drug effects / Q000502:physiology; D010058:Oviposition / Q000187:drug effects / Q000502:physiology
D002136:Calcium, Dietary; D004785:Environmental Pollutants; D002118:Calcium