Rapid diagnosis in early stage renal tuberculosis by real-time polymerase chain reaction on renal biopsy specimens.
OBJECTIVE
To determine the early rapid diagnosis of renal tuberculosis (RTB) by real-time polymerase chain reaction (PCR) on renal biopsy specimens.


METHODS
Ninety patients were selected for this study. The patients were divided into the following three groups: RTB, non-RTB (N-RTB) and clinically suspected RTB (CS-RTB). The renal biopsy specimens of these patients were used for Mycobacterium tuberculosis DNA detection by real-time PCR, using 35 and 40 as cycle threshold (C(T)) cut-off values. Morning urine samples were collected for M. tuberculosis culture.


RESULTS
In the RTB group, 25 C(T)35 and 28 C(T)40 patients were PCR-positive, seven of whom were urine M. tuberculosis culture-positive. In the N-RTB group, four C(T)35 and 13 C(T)40 patients were PCR-positive, none of whom were urine M. tuberculosis culture-positive. In the CS-RTB group, nine C(T)35 and 14 C(T)40 patients were PCR-positive, two of whom were urine M. tuberculosis culture-positive during 12 months of follow-up. The sensitivity and specificity of real-time PCR (C(T)40) were respectively 93.3% and 56.7%. The sensitivity and specificity of real-time PCR (C(T)35) were respectively 83.3% and 86.7%. The sensitivity and specificity of the urine M. tuberculosis culture were respectively 23.3% and 100%.


CONCLUSIONS
The detection of M. tuberculosis DNA in renal biopsy tissue by real-time PCR is highly sensitive. Real-time PCR can increase diagnostic accuracy and provide valuable information regarding the early diagnosis of RTB.
Sun
L
L
Department of Nephrology, First Affiliated Hospital of China Medical University, Shenyang, China.
Yuan
Q
Q
Feng
J-M
JM
Yang
C-M
CM
Yao
L
L
Fan
Q-L
QL
Liu
L-L
LL
Ma
J-F
JF
Wang
L-N
LN
D000328:Adult; D001706:Biopsy / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014398:Tuberculosis, Renal / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
D004269:DNA, Bacterial