A novel molecularly imprinted solid-phase microextraction fiber coupled with high performance liquid chromatography for analysis of trace estrogens in fishery samples.
The combination of molecular imprinting and solid-phase microextraction (SPME) technique provides a powerful sample preparation tool in terms of selectivity, simplicity, and flexibility. This paper reports a novel molecularly imprinted polymer (MIP) coated SPME fiber with 17beta-estradiol as template by improved multiple co-polymerization method. The obtained fiber exhibits excellent characteristics such as high porosity, good thermal and chemical stability. Extraction performance shows that the MIP-coated fiber has stronger affinity to the template molecule as compared with the commercial SPME fibers and the control polymer-coated fiber without addition of template. Owing to the shape and structural compatibility, the obtained fiber also demonstrated specific selectivity to the structural related compounds of 17beta-estradiol, such as estriol, estrone and 17 alpha-ethynylestradiol, and thus can be applied to simultaneous determination of these estrogens from complex samples coupled with high performance liquid chromatography. The variables that influence extraction were investigated. The MIP-coated fiber demonstrated its efficiency for extraction of estrogens in fishery samples. The detection limits were in the range of 0.98-2.39 microg L(-1), and the recoveries were 80.0-83.6% and 85.0-94.1% for fish and shrimp tissue samples, respectively.
School of Chemistry and Chemical Engineering, Sun Yat-sen University, 135 Xingang Xi Road, Guangzhou 510275, China.
Yuling
Y
Hu
Yangyang
Y
Wang
Xiaoguang
X
Chen
Yufei
Y
Hu
Gongke
G
Li
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004958:Estradiol / Q000032:analysis / Q000302:isolation & purification; D004967:Estrogens / Q000032:analysis* / Q000302:isolation & purification; D005398:Fisheries; D005399:Fishes; D057230:Limit of Detection; D054802:Molecular Imprinting; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
D004967:Estrogens; D004958:Estradiol