[Bioinformatics analysis and function prediction of the novel gene AY358935].
OBJECTIVE
To obtain the functional information of AY358935 gene.


METHODS
The properties, subcellular location, and structure of AY358935 protein, and the expression profile of AY358935 gene were analyzed by bioinformatics software and the biological functions of the gene were predicted. AY358935 expression was detected by Western blot analysis in early virus infection.


RESULTS
AY358935 was evolutionally conserved. The human AY358935 protein had an amino acid similarity of 74%, 60%, 38% and 33% with its counterpart in horses, mice, zebrafish and Xenopus laevis, respectively. Bioinformatics analysis indicated that AY358935 protein was located likely in the mitochondria. There was a N-terminal signal peptide and single transmembrane structure in AY358935 protein, which contained several phosphorylation sites. The secondary structure mainly comprised of alpha helices and random coils. AY358935 was ubiquitously expressed in normal tissues and carcinomas and regulated by the expression of double-stranded RNA-dependent protein kinase. AY358935 protein expression was obviously upregulated in cells 2 h after infection by vesicular stomatitis virus.


CONCLUSIONS
As a predicted secretary protein with a small molecular weight, AY358935 might have important functions in cellular proliferation and anti-viral innate immune regulation.
Xiong
Shao-quan
SQ
Department of Oncology, First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China. xsq106@yahoo.com.cn
Yang
Han-shuo
HS
Long
Qi-da
QD
D000595:Amino Acid Sequence; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012984:Software; D054243:Vesicular Stomatitis / Q000378:metabolism*
D011506:Proteins