Aligning a new reference genetic map of Lupinus angustifolius with the genome sequence of the model legume, Lotus japonicus.
We have developed a dense reference genetic map of Lupinus angustifolius (2n = 40) based on a set of 106 publicly available recombinant inbred lines derived from a cross between domesticated and wild parental lines. The map comprised 1090 loci in 20 linkage groups and three small clusters, drawing together data from several previous mapping publications plus almost 200 new markers, of which 63 were gene-based markers. A total of 171 mainly gene-based, sequence-tagged site loci served as bridging points for comparing the Lu. angustifolius genome with the genome sequence of the model legume, Lotus japonicus via BLASTn homology searching. Comparative analysis indicated that the genomes of Lu. angustifolius and Lo. japonicus are highly diverged structurally but with significant regions of conserved synteny including the region of the Lu. angustifolius genome containing the pod-shatter resistance gene, lentus. We discuss the potential of synteny analysis for identifying candidate genes for domestication traits in Lu. angustifolius and in improving our understanding of Fabaceae genome evolution.
Nelson
Matthew N
MN
School of Plant Biology and International Centre for Plant Breeding Education and Research, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. mnelson@plants.uwa.edu.au
Moolhuijzen
Paula M
PM
Boersma
Jeffrey G
JG
Chudy
Magdalena
M
Lesniewska
Karolina
K
Bellgard
Matthew
M
Oliver
Richard P
RP
Swiecicki
Wojciech
W
Wolko
Bogdan
B
Cowling
Wallace A
WA
Ellwood
Simon R
SR
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000737:chemistry; D030541:Databases, Genetic; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D000070116:Lotus / Q000235:genetics*; D031297:Lupinus / Q000235:genetics*; D016415:Sequence Alignment; D026801:Synteny
D018744:DNA, Plant