On the selection of relevant environmental factors to predict microbial dynamics in solidified media.
Several studies have shown that food structure causes slower growth rates and narrower growth boundaries of bacteria compared to laboratory media. In predictive microbiology, both a(w) or corresponding solute concentration (mainly NaCl) have been used as a growth influencing factor for kinetic models or growth/no growth interface models. The majority of these models have been based on data generated in liquid broth media with NaCl as the predominant a(w) influencing solute. However, in complex food systems, other a(w) influencing components might be present, next to NaCl. In this study, the growth rate of Salmonella typhimurium was studied in the growth region and the growth/no growth response was tested in Tryptic Soy Broth at 20 degrees C at varying gelatin concentration (0, 10, 50 g L(-1) gelatin), pH (3.25-5.5) and water activity (a(w)) (0.929-0.996). From the viewpoint of water activity, the results suggest that NaCl is the main a(w) affecting compound. However, gelatin seemed to have an effect on medium a(w) too. Moreover, there is also an interaction effect between NaCl and gelatin. From the microbial viewpoint, the results confirmed that the a(w) decreasing effect of gelatin is less harmful to cells than the effect of Na(+) ions. The unexpected shift of the growth/no growth interface to more severe conditions when going from a liquid medium to a medium with 10 g L(-1) gelatin is more pronounced when formulating the models in terms of a(w) than in terms of NaCl concentrations. At 50 g L(-1) gelatin, the model factored with NaCl concentration shifts to milder conditions (concordant to literature results) while the model with a(w) indicates a further shift to more severe conditions, which is due to the water activity lowering effect of gelatin and the interaction between gelatin and NaCl. The results suggest that solute concentration should be used instead of a(w), both for kinetic models in the growth region and for growth/no growth interface models, if the transferability of models to solid foods is to be increased.
Theys
T E
TE
Chemical and Biochemical Process Technology and Control Section (BioTeC), Department of Chemical Engineering, Katholieke Universiteit Leuven, Belgium.
Geeraerd
A H
AH
Devlieghere
F
F
Van Impe
J F
JF
D003470:Culture Media / Q000737:chemistry; D004777:Environment; D005516:Food Microbiology; D007700:Kinetics; D008954:Models, Biological; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012965:Sodium Chloride / Q000032:analysis; D014867:Water / Q000378:metabolism
D003470:Culture Media; D014867:Water; D012965:Sodium Chloride