[Screening specific allergens of allergic asthma from DNA expression library of Humulus pollen].
OBJECTIVE
To screen the allergic asthma-specific allergen from complementary DNA (cDNA) expression library of Humulus pollen.


METHODS
Screened the Humulus pollen lambdaTripIEx2 cDNA expression library which had been constructed using the method of immunological screening with patients' blood serum of Humulus pollen allergic asthma, extracted the positive phage DNA and the positive phage DNA, and identified by double restriction enzyme with EcoRI, Hind III and agarose gel electrophoresis. Then the DNA fragments were sequenced and the repeats and sequence homology were analyzed.


RESULTS
After 3 rounds of screening, three specific allergic cDNA clone of Humulus pollen were obtained and the sequences were 868, 550 and 592 base pairs respectively. The results of sequencing showed that these three clones represented different cDNA sequences. The repeats analysis found no repeats and these clones had no high homology with any known gene.


CONCLUSIONS
Three specific allergic cDNA clones of Humulus pollen obtained may be new genes. It provides a rational basis for constructing a recombinant allergen or nuclear acid vaccine.
Department of Respiratory Diseases, Second Affilliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
Jing
J
Xu
Xiu-zhen
XZ
Sun
Yun
Y
Liu
Bian-ling
BL
Yin
Yong-hong
YH
Zhang
Wei
W
Li
D052179:Antigens, Plant / Q000235:genetics / Q000302:isolation & purification*; D018076:DNA, Complementary; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D027582:Humulus / Q000235:genetics*; D008969:Molecular Sequence Data; D011058:Pollen / Q000235:genetics*
D052179:Antigens, Plant; D018076:DNA, Complementary; D018744:DNA, Plant