Complete nucleic acid sequence of Penaeus monodon densovirus (PmDNV) from India.
The complete nucleic acid sequence of the Penaeus monodon densovirus (PmDNV) from India was characterized. Analysis of the whole genome, consisting of 6310 bp revealed the presence of three open reading frames (ORFs), comprising 1281 bp, 1734 bp and 2460 bp, respectively. The complete genome and amino acid sequences of three proteins viz., NS1, NS2 and VP were compared with PmDNV from Thailand, PmergDNV from Australia and other partial sequences in GenBank, respectively. Highest nucleotide similarity was observed with the Thai strain (88%), while 33, 32 and 91 amino acid substitutions were observed in the NS2, NS1 and VP, respectively. Phylogenetic analysis of shrimp, insect and vertebrate parvovirus sequences revealed that the Indian PmDNV is more closely related to Thai isolates than all other parvoviruses reported so far.
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences, University, College of Fisheries, Mangalore-575 002, India.
Muhammed P
MP
Safeena
Anuj
A
Tyagi
Praveen
P
Rai
Iddya
I
Karunasagar
Indrani
I
Karunasagar
D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D017990:Densovirus / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D005838:Genotype; D007194:India; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033561:Penaeidae / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins