Scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus Scardovia and Scardovia inopinata.
Six strains of anaerobic, pleomorphic Gram-positive bacilli, isolated from the human oral cavity and an infected arm wound, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16S rRNA gene sequence analysis revealed that the isolates were most closely related to Scardovia inopinata CCUG 35729(T) (94.8-94.9 % 16S rRNA gene sequence similarity). The isolates were saccharolytic and produced acetic and lactic acids as end products of fermentation. The major fatty acids were C(16 : 0) (49.8 %) and C(18 : 1)ω9c (35.8 %). Polar lipid analysis revealed a variety of glycolipids, diphosphatidylglycerol, an unidentified phospholipid and an unidentified phosphoglycolipid. No respiratory quinones were detected. The peptidoglycan was of the type A4α L-Lys-Thr-Glu, with L-lysine partially replaced by L-ornithine. The DNA G+C content of one of the strains, C1A_55(T)(,) was 55 mol%. A novel species, Scardovia wiggsiae sp. nov., is proposed to accommodate the six isolates, with the type strain C1A_55(T) (=DSM 22547(T)=CCUG 58090(T)).
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, London, SE1 9RT, UK.
Julia
J
Downes
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, London, SE1 9RT, UK.
Maria
M
Mantzourani
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, London, SE1 9RT, UK.
David
D
Beighton
Cardiff University School of Dentistry, Heath Park, Cardiff, CF14 4XY, UK.
Samuel
S
Hooper
Cardiff University School of Dentistry, Heath Park, Cardiff, CF14 4XY, UK.
Melanie J
MJ
Wilson
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Ainsley
A
Nicholson
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, London, SE1 9RT, UK.
William G
WG
Wade
D019342:Acetic Acid / Q000378:metabolism; D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D019342:Acetic Acid