[Association of ENA-78, IP-10 and VEGF gene polymorphism with idiopathic pulmonary fibrosis].
OBJECTIVE
To examine whether there was an association between epithelial neutrophil activating peptide 78 (ENA-78), interferon-inducible protein 10 (IP-10), vascular endothelial growth factor (VEGF) polymorphism and Chinese patients with idiopathic pulmonary fibrosis (IPF).


METHODS
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to analyze the gene polymorphisms of ENA-78 (-156G/C), IP-10 (-1596C/T) and VEGF (+405G/C) in 60 IPF patients and 60 controls with trauma or bone fracture matched in age, gender and smoking status.


RESULTS
The distribution of carrying GC + CC genotype frequency (20.0%) and C allele frequency (12.7%) for ENA-78 in IPF patients was significantly higher than that in healthy controls [6.7% (P = 0.032) and 3.3% (P = 0.008), respectively], the relative risk of suffering from IPF of -156C allele gene carrier significantly increased (OR = 4.23, 95%CI: 1.35-13.20). The distribution of carrying CT + TT genotype frequency (10.0%) and T allele frequency (5.8%) for IP-10 in IPF patients was significantly lower than that in healthy controls [26.7% (P = 0.018) and 14.2% (P = 0.031), respectively], the relative risk of suffering from IPF of -1596T allele gene carrier decreased (OR = 0.38, 95%CI: 0.15-0.95). No association was found between VEGF (+405G/C) polymorphism and IPF.


CONCLUSIONS
-156C allele for ENA-78 may be a risk factor of IPF and -1596T allele for IP-10 a beneficial factor of IPF. The VEGF (+405G/C) gene polymorphism has no effect upon the predisposition to IPF.
Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Diseases, Beijing Chao-yang Hospital, Capital Medical University, Beijing 100020, China.
Long
L
Liu
Hua-Ping
HP
Dai
Bai
B
Xiao
Shu
S
Zhang
Cheng-Jun
CJ
Ban
Ping
P
Xin
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D054357:Chemokine CXCL10 / Q000235:genetics*; D054365:Chemokine CXCL5 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
C114753:CXCL10 protein, human; C486263:CXCL5 protein, human; D054357:Chemokine CXCL10; D054365:Chemokine CXCL5; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A