The +49A>G CTLA-4 polymorphism is associated with rheumatoid arthritis in Mexican population.
BACKGROUND
The Cytotoxic T lymphocyte antigen (CTLA-4) is one of the major susceptibility genes associated with autoimmune diseases. Susceptibility to rheumatoid arthritis (RA) is determined by both environmental and genetic factors. The genetic contribution approaches 50-60%. The association between RA with the +49A>G CTLA-4 polymorphism in the Mexican population was investigated.


METHODS
The polymerase chain reaction-restriction fragment was used to amplify the +49A>G CTLA-4 polymorphism in RA patients and healthy subjects (HS).


RESULTS
We analyzed the association between the +49A>G CTLA-4 polymorphism and RA. The G allele frequency was higher in RA patients than HS (46.8 vs 37.7%, OR=1.45, p=0.01). RA patients carrying the A/G genotype were significantly more likely to be positive to CRP and RF. There was no evidence of an association between SNP genotypes and the clinical characteristics of rheumatoid arthritis.


CONCLUSIONS
The +49A>G CTLA-4 polymorphism is a genetic marker of susceptibility for RA in western Mexican population.
Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. biologiamolecular@hotmail.com
José F
JF
Muñoz-Valle
Yeminia
Y
Valle
Jorge R
JR
Padilla-Gutiérrez
Isela
I
Parra-Rojas
Hector
H
Rangel-Villalobos
Mónica
M
Vázquez del Mercado
Iris Y
IY
Ledezma-Lozano
José R
JR
Villafan-Bernal
Juan
J
Armendáriz-Borunda
Ana Laura
AL
Pereira-Suárez
D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D060908:CTLA-4 Antigen; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012217:Rheumatoid Factor / Q000097:blood
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D002097:C-Reactive Protein; D012217:Rheumatoid Factor