Comparison of two different isolation methods of benzimidazoles and their metabolites in the bovine liver by solid-phase extraction and liquid chromatography-diode array detection.
A new analytical method that uses high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the analysis of 14 benzimidazoles residues, including metabolites, in bovine liver. Samples were extracted using two different extraction procedures: with phosphate buffer after enzymatic hydrolysis (method A) or using organic solvent, i.e. acetonitrile (method B). Then, samples were purified on a strong cation exchange (SCX) cartridge and analyzed in HPLC/DAD. The recovery percentages, obtained spiking the matrix (liver) at concentrations of 500 and 100 microg kg(-1) with a standard mixture of benzimidazoles, were in the range 6-101% and 80-102% for methods A and B, respectively. The repeatability of the methods was assessed in all cases by the % of correlation value (CV) that was lower than 19%. The limits of quantification (LOQs) in the matrix for methods A and B were in the range 40-60 and 20-50 microg kg(-1), respectively. The best of the two methods, method B, was used for the analysis of 10 bovine liver samples.
Caprioli
Giovanni
G
Dipartimento di Scienze Chimiche, Facoltà di Farmacia, Università di Camerino, via S. Agostino 1, Camerino, Italy.
Cristalli
Gloria
G
Galarini
Roberta
R
Giacobbe
Dania
D
Ricciutelli
Massimo
M
Vittori
Sauro
S
Zuo
Yanting
Y
Sagratini
Gianni
G
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001562:Benzimidazoles / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
D000097:Acetonitriles; D001562:Benzimidazoles; D010710:Phosphates; D010447:Peptide Hydrolases; C032159:acetonitrile