Effects of magnetic nanoparticle of Fe3O4 on apoptosis induced by Gambogic acid in U937 leukemia cells.
This study was aimed to explore the potential therapy of Gambogic acid (GA) combined with magnetic nanoparticle of Fe3O4 (Fe3O4-MNP) on leukemia. The proliferation of U937 cells and the cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscopy and flow cytometry respectively. The expressions of gene and protein were detected by quantitative real-time polymerase chain reaction and Western blot respectively. The results showed that GA enhanced the cytotoxicity for U937 cells in dose- and time-dependent manners. The Fe3O4-MNP itself had not cytotoxicity, but could enhance the inhibitory effect of GA on proliferation of U937 cells. The apoptotic rate of U937 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The typical apoptotic features of cells treated with GA and Fe3O4-MNP were observed. The expression levels of caspase-3 and bax after co-treatment of GA and Fe3O4-MNP were higher than that exposed to GA or Fe3O4-MNP alone, but the expressions of bcl-2, NF-kappaB and survivin were down-regulated. It is concluded that Fe3O4-MNP can promote GA-induced apoptosis in U937 cells, and the combination of GA with Fe3O4-MNP may be a safer and less toxic new therapy for leukemia.
Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical School, Nanjing 210009, Jiangsu Province, China.
Yi-Qiong
YQ
Liang
Bao-An
BA
Chen
Wei-Wei
WW
Wu
Feng
F
Gao
Guo-Hua
GH
Xia
Ze-Ye
ZY
Shao
Jian
J
Cheng
Jia-Hua
JH
Ding
Chong
C
Gao
Guo-Hong
GH
Li
Wen-Ji
WJ
Chen
Ning-Na
NN
Chen
Wen-Lin
WL
Xu
Xin-Chen
XC
Sun
Li-Jie
LJ
Liu
Xiao-Mao
XM
Li
Xue-Mei
XM
Wang
D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D058085:Iron Compounds / Q000008:administration & dosage* / Q000494:pharmacology*; D008280:Magnetics; D053758:Nanoparticles; D020298:U937 Cells; D044004:Xanthones / Q000494:pharmacology*
D058085:Iron Compounds; D044004:Xanthones; C052659:gambogic acid