The Arabidopsis defense component EDM2 affects the floral transition in an FLC-dependent manner.
Arabidopsis thaliana EDM2 was previously shown to be specifically required for disease resistance mediated by the R protein RPP7. Here we provide additional data showing that the role of EDM2 in plant immunity is limited and does not include a function in basal defense. In addition, we found that EDM2 has a promoting effect on the floral transition. We further found that the protein kinase WNK8 physically interacts with EDM2 in the nucleus. Unlike EDM2, which serves as a substrate of this kinase, WNK8 appears not to be required for RPP7-mediated defense. As reported previously, however, WNK8 does affect flowering time. Epistasis analyses suggested that EDM2 acts upstream of the floral repressor FLC (AT5G10140) and downstream of WNK8 (AT5G41990) in a regulatory module that resembles the autonomous floral promotion pathway, comprising a set of mechanisms that are known to affect the floral transition by regulating FLC transcript levels.
Tsuchiya
Tokuji
T
Center for Plant Cell Biology, Institute for Integrative Genome Biology, Department of Botany and Plant Sciences, University of California at Riverside, Riverside, CA 92521, USA.
Eulgem
Thomas
T
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic; D035264:Flowers / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D026161:MADS Domain Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C523205:EDM2 protein, Arabidopsis; C418902:At5g10140 protein, Arabidopsis; D026161:MADS Domain Proteins; D018749:RNA, Plant; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; C508913:WNK8 protein, Arabidopsis