Morphology of dopaminergic amacrine cells in the mouse retina: independence from homotypic interactions.
To determine the role of homotypic interactions between neighboring dopaminergic amacrine (DA) cells upon dendritic morphogenesis, the morphology of single cells was examined relative to the positioning of all neighboring homotypic cells. For each labeled cell, the dendritic field was reconstructed, its Voronoi domain was calculated, and the two were related. The dendritic fields of DA cells were observed to be large, sparse, and highly irregular. Dendrites readily overlapped those of neighboring cells, showing no evidence for dendritic tiling or inter-digitation consistent with homotypic repulsion or avoidance. Furthermore, a direct comparison of dendritic field area with the Voronoi domain area of the same cell showed no evidence for dendritic growth being constrained or biased by the local distribution of homotypic neighbors in wild-type retinas. A comparison of the processes of adjacent filled cells confirmed their immediate proximity to one another within the inner plexiform layer, indicating that they do not engage in mutual avoidance by coursing at different depths. Together, these results suggest that the morphogenesis of DA cells is independent of homotypic interactions. However, in the absence of the pro-apoptotic Bax gene, which yields a fourfold increase in DA cell number, a small but significant reduction in dendritic field size was obtained, although not so great as would be predicted by the increase in density. The present results are considered in light of recent studies on the role of cell adhesion molecules expressed by developing DA cells.
Keeley
Patrick W
PW
Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California 93106-9625, USA.
Reese
Benjamin E
BE
D025042:Amacrine Cells / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D002450:Cell Communication; D049108:Cell Enlargement; D003712:Dendrites / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012160:Retina / Q000166:cytology* / Q000502:physiology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein
D004298:Dopamine; D008164:Luminescent Proteins; D051028:bcl-2-Associated X Protein; D000097573:Red Fluorescent Protein; C494827:Bax protein, mouse