Generation of DNA profiles from fabrics without DNA extraction.
DNA profiles can be obtained from fabrics where a person has made direct contact with clothing. A standard approach is to cut out a section of the fabric and then use a commercially available method to extract and isolate the DNA. Alternative methods to isolate DNA include the use of adhesive tape to remove traces of cellular material from the fabric prior to extraction. We report on a process to obtain full DNA profiles using direct amplification from a range of fabrics. The absence of an extraction step both reduces the opportunity for contamination and reduces the loss of DNA during the extraction process, increasing the sensitivity of the process of generating a DNA profile. The process does not require the use of commercially available extraction kits thus reducing the cost of generating a DNA profile from trace amounts of starting material. The results are in part dependent upon the nature of the fabric used to which the DNA has been transferred.
Linacre
Adrian
A
Centre for Forensic Science, WestChem, University of Strathclyde, Glasgow G1 1XW, UK. a.m.t.linacre@strath.ac.uk
Pekarek
Vera
V
Swaran
Yuvaneswari Chandramoulee
YC
Tobe
Shanan S
SS
D003020:Clothing; D032561:Cotton Fiber; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000191:economics / Q000379:methods*; D004355:Drug Stability; D004586:Electrophoresis; D005554:Forensic Medicine / Q000379:methods; D005784:Gene Amplification; D006801:Humans; D009757:Nylons; D011091:Polyesters; D016133:Polymerase Chain Reaction / Q000379:methods*; D013784:Textiles / Q000032:analysis*
D009757:Nylons; D011091:Polyesters; D004247:DNA