Acute metal stress in Populus tremula x P. alba (717-1B4 genotype): leaf and cambial proteome changes induced by cadmium 2+.
The comprehension of metal homeostasis in plants requires the identification of molecular markers linked to stress tolerance. Proteomic changes in leaves and cambial zone of Populus tremula x P. alba (717-1B4 genotype) were analyzed after 61 days of exposure to cadmium (Cd) 360 mg/kg soil dry weight in pot-soil cultures. The treatment led to an acute Cd stress with a reduction of growth and photosynthesis. Cd stress induced changes in the display of 120 spots for leaf tissue and 153 spots for the cambial zone. It involved a reduced photosynthesis, resulting in a profound reorganisation of carbon and carbohydrate metabolisms in both tissues. Cambial cells underwent stress from the Cd actually present inside the tissue but also a deprivation of photosynthates caused by leaf stress. An important tissue specificity of the response was observed, according to the differences in cell structures and functions.
Durand
Thomas C
TC
CRP-Gabriel Lippmann, Department Environment and Agro-biotechnologies, Belvaux, GD, Luxembourg.
Sergeant
Kjell
K
Planchon
Sébastien
S
Carpin
Sabine
S
Label
Philippe
P
Morabito
Domenico
D
Hausman
Jean-François
JF
Renaut
Jenny
J
D002104:Cadmium / Q000378:metabolism*; D003433:Crosses, Genetic; D005838:Genotype; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism*; D032107:Populus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological / Q000235:genetics; D013997:Time Factors
D020543:Proteome; D002104:Cadmium