Application of point-to-point matching algorithms for background correction in on-line liquid chromatography-Fourier transform infrared spectrometry (LC-FTIR).
A new background correction method for the on-line coupling of gradient liquid chromatography and Fourier transform infrared spectrometry has been developed. It is based on the use of a point-to-point matching algorithm that compares the absorption spectra of the sample data set with those of a previously recorded reference data set in order to select an appropriate reference spectrum. The spectral range used for the point-to-point comparison is selected with minimal user-interaction, thus facilitating considerably the application of the whole method. The background correction method has been successfully tested on a chromatographic separation of four nitrophenols running acetonitrile (0.08%, v/v TFA):water (0.08%, v/v TFA) gradients with compositions ranging from 35 to 85% (v/v) acetonitrile, giving accurate results for both, baseline resolved and overlapped peaks.
Department of Analytical Chemistry, Universitat de València, Edificio Jeroni Muñoz, 50th Dr Moliner, 46100 Burjassot, Spain.
J
J
Kuligowski
G
G
Quintás
S
S
Garrigues
M
M
de la Guardia
D000465:Algorithms; D002853:Chromatography, Liquid / Q000379:methods*; D009596:Nitrophenols / Q000032:analysis / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D009596:Nitrophenols