Immunoassays for collagenase-mediated cleavage of type I and II collagens.
This chapter describes the production and characterization of antibodies raised against neoepitopes in collagenase-cleaved collagen. It also details the development, validation, and use of immunoassays using such antibodies to measure specifically collagenase-mediated cleavage.
School of Applied Sciences, St. Lawrence College, Kingston, ON, Canada.
R Clark
RC
Billinghurst
Mirela
M
Ionescu
A Robin
AR
Poole
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism*; D024043:Collagen Type II / Q000737:chemistry / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000379:methods*; D008969:Molecular Sequence Data; D012015:Reference Standards
D000906:Antibodies; D015415:Biomarkers; D024042:Collagen Type I; D024043:Collagen Type II; D017364:Collagenases