Lack of a central role for osteoprogenitor cells from the femoral canal in heterotopic ossification of the hip: an experimental study in a rat model.
We have developed an animal model to examine the formation of heterotopic ossification using standardised muscular damage and implantation of a beta-tricalcium phosphate block into a hip capsulotomy wound in Wistar rats. The aim was to investigate how cells originating from drilled femoral canals and damaged muscles influence the formation of heterotopic bone. The femoral canal was either drilled or left untouched and a tricalcium phosphate block, immersed either in saline or a rhBMP-2 solution, was implanted. These implants were removed at three and 21 days after the operation and examined histologically, histomorphometrically and immunohistochemically. Bone formation was seen in all implants in rhBMP-2-immersed, whereas in those immersed in saline the process was minimal, irrespective of drilling of the femoral canals. Bone mineralisation was somewhat greater in the absence of drilling with a mean mineralised volume to mean total volume of 18.2% (sd 4.5) versus 12.7% (sd 2.9, p < 0.019), respectively. Our findings suggest that osteoinductive signalling is an early event in the formation of ectopic bone. If applicable to man the results indicate that careful tissue handling is more important than the prevention of the dissemination of bone cells in order to avoid heterotopic ossification.
Department of Traumatology and Orthopaedics University of Tartu, Puusepa 8, Tartu 51014, Estonia. alar.toom@ut.ee
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Toom
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Suutre
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Märtson
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Haviko
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Selstam
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Arend
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D004195:Disease Models, Animal; D005269:Femur / Q000473:pathology; D006621:Hip Joint / Q000473:pathology*; D008297:Male; D009999:Ossification, Heterotopic / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000502:physiology*
D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D015675:Osteocalcin