Application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue.
The present study demonstrates an application of Parallux (a solid-phase fluorescence immunoassay) for amoxicillin analysis in fish tissue. Amoxicillin at the recommended therapeutic dose (400 mg/kg body weight) was orally administered to three groups of 25 olive flounder ( Paralichthys olivaceus ), 25 rockfish ( Sebastes schlegeli ) and 25 red sea bream ( Pagrus major ) for 7 consecutive days. Amoxicillin was detected in the muscle of fish treated by the 3rd day of the withdrawal period. The recovery rates of all spiked muscle samples were > 86% of the spiked values. The present study showed that solid-phase fluorescence immunoassay can be easily adopted in predicting amoxicillin residues in the muscle tissue of farmed fish.
Park
Eun-Kee
EK
University of Occupational and Environmental Health Department of Environmental Epidemiology, Institute of Industrial Ecological Sciences Kitahyushu Japan.
Jung
Won Chul
WC
Lee
Hu-Jang
HJ
D000658:Amoxicillin / Q000008:administration & dosage / Q000032:analysis*; D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000658:Amoxicillin