Role of RECK methylation in gastric cancer and its clinical significance.
OBJECTIVE
To investigate the relation between RECK methylation and clinicopathological characteristics of gastric cancer patients and evaluate the role of RECK methylation in peritoneal metastasis of gastric cancer.


METHODS
Methylation of RECK gene in 40 paired samples of gastric cancer and its corresponding adjacent normal mucosa, lymph nodes and peritoneal irrigation fluid was detected by methylation-specific polymerase chain reaction.


RESULTS
Aberrant methylation of RECK gene was detected in 27.5% (11/40) of the adjacent normal mucosa samples, in 47.5% (19/40) of gastric cancer samples, in 57.1% (12/21) of the lymph node samples, and in 35% (14/40) of peritoneal irrigation fluid samples, respectively, with a significant difference between the adjacent normal mucosa and lymph node samples (P = 0.023). Presence of RECK methylation in the primary tumor samples was significantly correlated with tumor invasion (P = 0.023). The accuracy of RECK methylation in peritoneal lavage fluid samples for the diagnosis of peritoneal metastasis of gastric cancer was 72.5% (26/40), with a sensitivity of 66.7% (6/9) and a specificity of 74.2% (23/31).


CONCLUSIONS
Aberrant methylation of RECK gene may provide useful information for the early diagnosis and treatment of peritoneal metastasis of gastric cancer.
Department of Surgical Oncology, the First Affiliated Hospital China Medical University, Shenyang 110001, Liaoning Province, China.
Yun-Yi
YY
Du
Dong-Qiu
DQ
Dai
Zhi
Z
Yang
D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics* / Q000556:secondary; D019175:DNA Methylation; D055088:Early Detection of Cancer; D005260:Female; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010533:Peritoneal Lavage; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000556:secondary; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C115460:RECK protein, human