Simian betaretrovirus infection in a colony of cynomolgus monkeys (Macaca fascicularis).
Of the 419 laboratory-bred cynomolgus macaques (Macaca fascicularis) in a breeding colony at our institution, 397 (95%) exhibited antibodies or viral RNA (or both) specific for simian betaretrovirus (SRV) in plasma. Pregnant monkeys (n= 95) and their offspring were tested to evaluate maternal-infant infection with SRV. At parturition, the first group of pregnant monkeys (n = 76) was antibody-positive but RNA-negative, the second group (n = 14 monkeys) was positive for both antibody and RNA, and the last group (n = 5) was antibody-negative but RNA-positive. None of the offspring delivered from the 76 antibody-positive/RNA-negative mothers exhibited viremia at birth. Eight of the offspring (including two newborns delivered by caesarian section) from the 14 dually positive mothers exhibited SRV viremia, whereas the remaining 6 newborns from this group were not viremic. All of the offspring (including 2 newborns delivered by caesarian section) of the 5 antibody-negative/RNA-positive mothers exhibited viremia at birth. One neonatal monkey delivered by CS and two naturally delivered monkeys that were viremic at birth remained viremic at 1 to 6 mo of age and lacked SRV antibodies at weaning. Family analysis of 2 viremic mothers revealed that all 7 of their offspring exhibited SRV viremia, 6 of which were also antibody-negative. The present study demonstrates the occurrence of transplacental infection of SRV in viremic dams and infection of SRV in utero to induce immune tolerance in infant monkeys.
Fujiomto
Koji
K
National Institute of Biomedical Innovation, Tsukuba, Japan. fujimoto@primate.or.jp
Takano
Jun-ichiro
J
Narita
Toyoko
T
Hanari
Koji
K
Shimozawa
Nobuhiro
N
Sankai
Tadashi
T
Yosida
Takashi
T
Terao
Keiji
K
Kurata
Takeshi
T
Yasutomi
Yasuhiro
Y
D000818:Animals; D030121:Betaretrovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005260:Female; D008252:Macaca fascicularis; D008297:Male; D011247:Pregnancy; D012367:RNA, Viral / Q000235:genetics; D014766:Viremia
D012367:RNA, Viral