[The expression of mucins gene in the human nasal polyps and allergic rhinitis].
OBJECTIVE
To detect the mucin gene (MUC2, MUC5AC, MUC5B, MUC18 and MUC19) expression in the nasal polyps, allergic rhinitis (AR) and the normal nasal mucosa in human. To investigate the role and clinical significance of mucin gene in the pathogenesis of nasal polyps and AR patients.


METHODS
We obtained samples from 35 cases of nasal polyps, 18 cases of AR inferior turbinate and 18 cases of simple nasal septum deviation inferior turbinate. Specimens were analyzed with RT-PCR and Real-time FQ-RT-PCR.


RESULTS
The results of RT-PCR and FQ-RT-PCR showed that the expression of MUC5AC, MUC5B in nasal polyps and AR patients was significantly higher than that in normal mucosa (P<0.05). The expression of MUC5AC, MUC5B in nasal polyps was not significantly different from that in AR patients (P>0.05). The expression of MUC2, MUC18 in nasal polyps and AR was not significantly different from that in normal mucosa (P>0.05). And the results of RT-PCR for MUC19 expression in AR was higher than that in nasal polyps group and normal group (P<0.05 or P<0.01).


CONCLUSIONS
MUC5AC and MUC5B are highly expressed in epithelium of human nasal polyps and AR, and they take part in mucus over-secretion in nasal polyps and AR. The expression of MUC19 in AR was higher than that in nasal polyps group and normal group. It indicates that the secretion of MUC19 in allergic rhinitis was on high level. There was no difference of the expression of MUC2 and MUC18 in nasal polyps group, AR group and in normal group.
Department of Otolaryngology, Sun Yat-sen Hospital of Xiamen University, Xiamen, 361004, China.
Chenqi
C
Ji
Yongqing
Y
Guo
D000293:Adolescent; D000328:Adult; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000235:genetics; D055262:Mucin-2 / Q000235:genetics; D055257:Mucin-5B / Q000235:genetics; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism; D012220:Rhinitis / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
C482349:MUC19 protein, human; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524578:MUC5B protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055257:Mucin-5B; D009077:Mucins