Biological evaluation of layered double hydroxides as efficient drug vehicles.
Recently there has been a rapid expansion of the development of bioinorganic hybrid systems for safe drug delivery. Layered double hydroxides (LDH), a variety of available inorganic matrix, possess great promise for this purpose. In this study, an oxidative stress biomarker system, including measurement of reactive oxygen species, glutathione content, endogenous nitric oxide, carbonyl content in proteins, DNA strand breaks and DNA-protein crosslinks, was designed to evaluate the biocompatibility of different concentrations of nano-Zn/Al-LDH with a Hela cell line. The drug delivery activity of the LDH-folic-acid complex was also assessed. The resulting data clearly demonstrated that nano-LDH could be applied as a relatively safe drug vehicle with good delivery activity, but with the caveat that the effects of high dosages observed here should not be ignored when attempting to maximize therapeutic activity by increasing LDH concentration.
Li
Yan
Y
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, People's Republic of China.
Liu
Dan
D
Ai
Hanhua
H
Chang
Qing
Q
Liu
Dandan
D
Xia
Ying
Y
Liu
Shuwen
S
Peng
Nanfang
N
Xi
Zhuge
Z
Yang
Xu
X
D017209:Apoptosis / Q000187:drug effects; D020552:Comet Assay; D004337:Drug Carriers / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D006878:Hydroxides / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D004337:Drug Carriers; D006878:Hydroxides; D017382:Reactive Oxygen Species