2-D RP/RPLC method to separate components in Fructus schisandrae chinensis.
An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click beta-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. Beta-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click beta-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click beta-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.
Jin
Gaowa
G
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Dai
Yuntao
Y
Feng
Jiatao
J
Qin
Xuemei
X
Xue
Xingya
X
Zhang
Feifang
F
Liang
Xinmiao
X
D002853:Chromatography, Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D032141:Schisandra / Q000737:chemistry*