[Influence of magnetic Fe3O4 nanoparticle on functions of lymphocytes and macrophages in mice].
This study was purposed to investigate the effects of magnetic nanoparticle of Fe3O4 (Fe3O4-MNPs) on murine immune system. ICR mice were assigned randomly into four groups which were treated with normal saline, low, middle and high dose of MNP-Fe3O4 respectively. The mice were killed after being exposed by intragastric administration for 2 weeks. The ratios of spleen weight to body weight, lymphocyte transformation rate in spleen suspension and phagocytic index of macrophage in abdominal cavity were detected. The results showed that the ratios of spleen weight to body weight in Fe3O4-MNP groups were not significantly different in comparison with the control (p > 0.05). The lymphocyte transformation rate in spleen suspension in Fe3O4-MNP groups were all higher than that in control group (-0.1775 +/- 0.0246), especially in the middle dose group (0.1833 +/- 0.0593) (p < 0.05), and the phagocytic index of macrophages in abdominal cavity of middle dose group (0.2051 +/- 0.0213) was higher than that of control group and other two Fe3O4-MNP group (low dose 0.1538 +/- 0.0100, high dose 0.1511 +/- 0.0184) (p < 0.05). It is concluded that suitable dose of Fe3O4-MNP can enhance the cellular immune activity and phagocytic function of macrophages of mice.
Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College; Nanjing 210009, Jiangsu Province, China.
Nan
N
Jin
Jun
J
Wang
Bao-An
BA
Chen
Jia-Hua
JH
Ding
Chong
C
Gao
Jian
J
Cheng
Gang
G
Zhao
Wen
W
Bao
Feng
F
Gao
Guo-Hua
GH
Xia
Yin
Y
Zhou
Yue
Y
Chen
Gui-Na
GN
Zhou
Xiao-Mao
XM
Li
Xue-Mei
XM
Wang
D000818:Animals; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis
D058185:Magnetite Nanoparticles