A pulsed-field gel electrophoresis study of the genetic diversity of Campylobacter jejuni and Campylobacter coli in poultry flocks in Slovenia.
Campylobacter jejuni and C. coli have recently become the most frequent cause of bacterial foodborne enteric infection in most industrialised countries. Consumption and handling of undercooked contaminated poultry meat was identified as an important risk factor for human campylobacteriosis. The aim of this study was to ascertain the genetic diversity of C. jejuni and C. coli strains isolated from poultry in Slovenia. A total of 68 isolates (42 C. jejuni , 26 C. coli ) from faeces (n = 48), meat (n = 15) and skin/carcasses (n = 5) of chicken (n = 60) and turkey samples (n = 5) were analysed by pulsed-field gel electrophoresis. Sma I macrorestriction discriminated between C. jejuni and C. coli isolates. C. jejuni isolates exhibited a higher degree of diversity compared to C. coli isolates. In the C. jejuni group, a number of small clusters were apparent, while C. coli strains formed less but larger clusters. Additional Kpn I digestion of selected isolates resulted in poor subtyping. Strains with identical or very similar profiles were found on different farms, either in the same or different regions and time periods. Some of the clones indicated possible cross-contamination at slaughterhouses.
University of Ljubljana Institute of Microbiology and Parasitology, Veterinary Faculty Gerbiceva 60 1000 Ljubljana Slovenia.
Igor
I
Gruntar
Matjaz
M
Ocepek
Jana
J
Avbersek
Jasna
J
Mićunović
Mateja
M
Pate
D000818:Animals; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017000:Campylobacter coli / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary*; D014644:Genetic Variation; D011200:Poultry; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D017524:Slovenia / Q000453:epidemiology