Evaluation of erythrocyte band 3 phosphotyrosine level, glutathione content, CA-125, and human epididymal secretory protein E4 as combined parameters in endometriosis.
OBJECTIVE
To investigate the biochemical parameters of the erythrocyte response to diamide-induced oxidative stress, alone or as adjuncts to serum values of CA-125 and human epididymal secretory protein E4 (HE4), in the diagnosis and study of endometriosis.


METHODS
University of Padova.


METHODS
Prospective study.


METHODS
Forty-five patients of reproductive age undergoing laparoscopy.


METHODS
All women were studied for endometriotic foci during laparoscopic surgery. Forty-one had laparoscopically and histologically confirmed endometriosis, and four did not. Twenty women with confirmed endometriosis were reassessed 1-4 months later.


METHODS
CA-125 and HE4 and two new parameters evaluated in erythrocytes after diamide-induced stress, that is, band 3 tyrosine phosphorylation (Tyr-P) level and decrease in total glutathione content (ΔGSH), were assessed in all patients.


RESULTS
In association with serum CA-125 levels but not with HE4, diamide-related erythrocyte band 3 Tyr-P and ΔGSH were significantly higher in patients with endometriosis and were able to discriminate with high sensitivity and specificity between patients before and after surgery.


CONCLUSIONS
Endometriosis is associated with an increase in systemic oxidative stress, affecting the antioxidative defenses of circulating erythrocytes. All related implications, including evaluation of other oxidative stress-related changes, warrant further study.
Department of Biological Chemistry, University of Padova, Padova, Italy.
Luciana
L
Bordin
Cristina
C
Fiore
Gabriella
G
Donà
Alessandra
A
Andrisani
Guido
G
Ambrosini
Diego
D
Faggian
Mario
M
Plebani
Giulio
G
Clari
Decio
D
Armanini
D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte; D015415:Biomarkers / Q000097:blood; D018394:CA-125 Antigen / Q000097:blood*; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis; D030501:Epididymal Secretory Proteins / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000097:blood*; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D019000:Phosphotyrosine / Q000097:blood*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014591:Uterine Diseases / Q000097:blood* / Q000175:diagnosis; D023083:beta-Defensins
D001457:Anion Exchange Protein 1, Erythrocyte; D015415:Biomarkers; D018394:CA-125 Antigen; C426184:DEFB126 protein, human; D030501:Epididymal Secretory Proteins; D023083:beta-Defensins; D019000:Phosphotyrosine; D005978:Glutathione