Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development.
Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT-PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development.
CSIRO Entomology, Canberra, ACT, 2601, Australia. Tony.Duan@csiro.au
Guowen
G
Duan
Robert B
RB
Saint
Chris A
CA
Helliwell
Carolyn A
CA
Behm
Peter M
PM
Waterhouse
Karl H J
KH
Gordon
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D018509:DNA, Helminth / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D005260:Female; D015870:Gene Expression; D017238:Genes, Helminth; D008297:Male; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology; D010802:Phylogeny; D034622:RNA Interference; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D011994:Recombinant Proteins; C406772:EGO-1 protein, C elegans; D012324:RNA-Dependent RNA Polymerase