Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord.
Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwann-cell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.
Patel
Vivek
V
The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, Florida 33101, USA.
Joseph
Gravil
G
Patel
Amit
A
Patel
Samik
S
Bustin
Devin
D
Mawson
David
D
Tuesta
Luis M
LM
Puentes
Rocio
R
Ghosh
Mousumi
M
Pearse
Damien D
DD
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery*; D013535:Suspensions / Q000008:administration & dosage / Q000737:chemistry
D013535:Suspensions