Glycome profiling using modern glycomics technology: technical aspects and applications.
Glycomics research has become indispensable in many research fields such as immunity, signal transduction and development. Moreover, changes in the glycosylation of proteins and lipids have been reported in several diseases including cancer. The analysis of a complex post-translational modification such as glycosylation depends on the availability or development of appropriate analytical technologies. The research goal determines the sensitivity, resolution and throughput requirements and guides the choice of a particular technology. This review highlights the evolution of glycan profiling tools in the past 5 years. We focus on capillary electrophoresis, liquid chromatography, mass spectrometry and lectin microarrays.
Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Technologiepark 927, B-9052 Ghent, Belgium.
Dieter
D
Vanderschaeghe
Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Technologiepark 927, B-9052 Ghent, Belgium.
Nele
N
Festjens
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, B-9000 Ghent, Belgium.
Joris
J
Delanghe
Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Technologiepark 927, B-9052 Ghent, Belgium.
Nico
N
Callewaert
D000818:Animals; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry; D046228:Microarray Analysis; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism
D011134:Polysaccharides