Myeloid translocation gene 16b is a dual A-kinase anchoring protein that interacts selectively with plexins in a phospho-regulated manner.
The myeloid translocation gene (MTG) homologue Nervy associates with PlexinA on the plasma membrane, where it functions as an A-kinase anchoring protein (AKAP) to modulate plexin-mediated semaphorin signaling in Drosophila. Mammalian MTG16b is an AKAP found in immune cells where plexin-mediated semaphorin signaling regulates immune responses. This study provides the first evidence that MTG16b is a dual AKAP capable of binding plexins. These interactions are selective (PlexinA1 and A3 bind MTG, while PlexinB1 does not) and can be regulated by PKA-phosphorylation. Collectively, these data suggest a possible mechanism for the targeting and integration of adenosine 3',5'-cyclic monophosphate (cAMP) and semaphorin signaling in immune cells.
VA Medical Center and Department of Endocrinology, Oregon Health & Sciences University, Portland, OR 97239, USA.
Sarah E
SE
Fiedler
Robynn V
RV
Schillace
Crystal J
CJ
Daniels
Sarah F
SF
Andrews
Daniel W
DW
Carr
D054758:A Kinase Anchor Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D054743:Cyclic AMP-Dependent Protein Kinase Type I / Q000235:genetics / Q000378:metabolism; D054746:Cyclic AMP-Dependent Protein Kinase Type II / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D054758:A Kinase Anchor Proteins; C115744:CBFA2T3 protein, human; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C492116:PLXNA1 protein, human; C097871:PLXNA3 protein, human; C492119:PLXNB1 protein, human; D010750:Phosphoproteins; C492114:Plxna1 protein, mouse; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; C091529:plexin; D054743:Cyclic AMP-Dependent Protein Kinase Type I; D054746:Cyclic AMP-Dependent Protein Kinase Type II