Evaluation of the transdermal permeation of different paraben combinations through a pig ear skin model.
Although parabens have several features of ideal preservatives, different studies have shown that they may affect human health due to their estrogenic activity. Therefore, various strategies have been applied to reduce their skin penetration. However, the effect of paraben combinations on transdermal permeation has not yet been investigated. Thus, the objective of this study was to evaluate paraben permeation in pig ear skin using a Franz diffusion cell system with capillary electrophoresis detection, in order to identify which paraben combinations (defined by a factorial design) have the lowest skin permeation. The permeation of isolated parabens was also evaluated and the permeation characteristics, obtained by the Moser model, confirmed that lipophilicity and molecular weight may influence the systemic absorption of these compounds. In previous tests using isolated parabens, methyl and ethyl parabens presented greater retention in the epidermis compared to the dermis, while propyl and butyl parabens had similar retention profiles in these layers. An increase in ethanol concentration and experimental time promoted greater parabens retention in the dermis compared to the epidermis. The binary combinations of methyl and ethyl parabens as well as of methyl and propyl parabens (added to several cosmetic products in order to increase the antimicrobial spectrum) reduced significantly their permeation rates through pig ear skin (with the exception of EP), probably due to the high retention of these parabens in the epidermis and dermis.
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil.
Thiago
T
Caon
Ana Carolina Oliveira
AC
Costa
Marcone Augusto Leal
MA
de Oliveira
Gustavo Amadeu
GA
Micke
Cláudia Maria Oliveira
CM
Simões
D000279:Administration, Cutaneous; D000818:Animals; D020405:Dermis / Q000378:metabolism; D004431:Ear, External / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D004817:Epidermis / Q000378:metabolism; D023421:Models, Animal; D010226:Parabens / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010539:Permeability; D012869:Skin Absorption; D012995:Solubility; D013552:Swine
D010226:Parabens