Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.
We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.
Bub
Gil
G
Department of Physiology Anatomy and Genetics, Oxford, UK. gil.bub@dpag.ox.ac.uk
Tecza
Matthias
M
Helmes
Michiel
M
Lee
Peter
P
Kohl
Peter
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted; D008856:Microscopy, Fluorescence; D051381:Rats; D012815:Signal Processing, Computer-Assisted
D002118:Calcium