The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors.
All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts.
Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark. jglahder@hotmail.com
Jacob A
JA
Glahder
Karen
K
Kristiansen
Marjorie
M
Durand
Jeppe
J
Vinther
Bodil
B
Norrild
D020413:3' Untranslated Regions; D019677:Artificial Gene Fusion; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D011062:Polynucleotide Adenylyltransferase; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism*
D020413:3' Untranslated Regions; C490386:CPEB1 protein, human; D012333:RNA, Messenger; D012367:RNA, Viral; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors; D008156:Luciferases; D011062:Polynucleotide Adenylyltransferase; C550174:TENT2 protein, human