Choosing the sequence of dsRNA for RNAi in mouse.
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.
Svoboda
Petr
P
Institute of Molecular Genetics, Academy of Sciences of Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. svobodap@img.cas.cz
D020413:3' Untranslated Regions; D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020868:Gene Silencing; D005821:Genetic Techniques; D051379:Mice; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering