Cloning of the gene encoding an endo-acting pectate lyase from Streptomyces thermocarboxydus.
An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative pectate lyase of S. coelicolor. Recombinant PL I had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis.
Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan. symbio@cc.hirosaki-u.ac.jp
Akio
A
Tonouchi
Yuichi
Y
Hara
Ryousuke
R
Umehara
Tamotsu
T
Sanuki
Takuma
T
Fukusawa
Kazuo
K
Miyairi
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D011133:Polysaccharide-Lyases / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase