Novel FKS mutations associated with echinocandin resistance in Candida species.
We studied three clinical isolates of Candida spp. (one C. tropicalis isolate and two C. glabrata isolates) from patients with invasive candidiasis. The first isolate emerged during echinocandin treatment, while the others emerged after the same treatment. These strains harbored an amino acid substitution in Fksp never linked before with reduced echinocandin susceptibility in C. tropicalis or in C. glabrata. The molecular mechanism of reduced susceptibility was confirmed using a 1,3-beta-D-glucan synthase inhibition assay.
Public Health Research Institute, Newark, New Jersey, USA.
Guillermo
G
Garcia-Effron
Daniel Joseph
DJ
Chua
Jon Rupert
JR
Tomada
Joseph
J
DiPersio
David S
DS
Perlin
Mahmoud
M
Ghannoum
Hector
H
Bonilla
D000328:Adult; D000935:Antifungal Agents / Q000627:therapeutic use*; D041221:Candida glabrata / Q000235:genetics*; D041022:Candida tropicalis / Q000235:genetics*; D002177:Candidiasis / Q000188:drug therapy* / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D054714:Echinocandins / Q000627:therapeutic use*; D005260:Female; D005964:Glucosyltransferases / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation
D000935:Antifungal Agents; D054714:Echinocandins; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase