[Human papillomavirus (HPV) genotypes in cervix uterine cancer patients in a public hospital and private clinic from Santiago, Chile].
We compared HPV genotypes among squamous cervical cancer samples from a public hospital (n = 55) and a private clinic (n = 35 cases) of Santiago. Paraffin-embedded specimens were analyzed by PCR followed by an immunoenzimatic assay. Reverse line blotting was used for the identification of 36 HPV genotypes. We found HPVDNAm 94.4% of all cancers. Single mfections: HPV16: 40.0%, (clinic 37.1%, hospital 41.8%) VPH18:7.8% (clinic 2.9%, hospital 10.9%); single+multiple mfections: VPH16: 61.1% (clinic 53.1%, hospital 71.7%), VPH18: 34.4% (clinic 21.9%, hospital 45.2%). HPV16 orHPV18 occurredin 75.6% of cases, higher in the hospital than the clinic (87.3%-95% CI: 84.9-96.3 - and 57. l%-95% CI: 46.6-66 - respectively, p = 0.002). Other genotypes in single mfections: HPV 26, 31, 33, 45, 58, 67; in co-mfections: HPV 35,52,56,59 and 66. HPV16 but specially HPV 18 were significantly more frequent in the public hospital; 75.6% of squamous cervical cancer were associated to the vaccine preventable HPV16/18.
Valdivia L
Isabel M
IM
Laboratorio de Patología Molecular y Epidemiología, Centro de Investigaciones Médicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Aguayo G
Francisco
F
Pruyas A
Martha
M
Snijders
Peter J F
PJ
Corvalán R
Alejandro
A
Ferreccio R
Catterina
C
D000328:Adult; D052685:Alphapapillomavirus / Q000235:genetics* / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D002677:Chile; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D017149:Private Sector; D017150:Public Sector; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral