Organelle-specific, rapid induction of molecular activities and membrane tethering.
Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.
Komatsu
Toru
T
Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Baltimore, Maryland, USA.
Kukelyansky
Igor
I
McCaffery
J Michael
JM
Ueno
Tasuku
T
Varela
Lidenys C
LC
Inoue
Takanari
T
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D019281:Dimerization; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D041681:NIH 3T3 Cells; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D058570:TOR Serine-Threonine Kinases; D022021:Tacrolimus Binding Proteins / Q000378:metabolism*
D047908:Intracellular Signaling Peptides and Proteins; C546842:MTOR protein, human; C546843:mTOR protein, mouse; D017346:Protein Serine-Threonine Kinases; D058570:TOR Serine-Threonine Kinases; D022021:Tacrolimus Binding Proteins