Single nucleotide polymorphism genotyping in MMP genes: the 5' nuclease assay.
Many molecular genetic studies of human diseases involve determining the genotypes for single nucleotide polymorphisms. This chapter summarises a number of different techniques for the single nucleotide polymorphism genotyping which can be applied to MMP genes. The chapter also provides a protocol and technical notes for the 5'-nuclease assay, one of the most commonly used genotyping techniques.
Barts and the London School of Medicine and Dentistry, William Harvey Research Institute, London, UK.
Ross
R
Laxton
Shu
S
Ye
D004247:DNA / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D005838:Genotype; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013223:Statistics as Topic
D004247:DNA; D003851:Deoxyribonucleases; D020782:Matrix Metalloproteinases