[Colorectal cancer: tissutal explantation and primary cell culture].
BACKGROUND
Setting of cellular cultures extracted from colorectal cancer tissue represents a valid model for in vitro study of biological and molecular characteristics of each single tumor finalized to obtain a tailored chemiotherapy. The end point of this study is to create primary cellular cultures from "fresh" cancer tissue in different stages of evolution.


METHODS
Cancer tissue samples are obtained by means of surgical excisional biopsy or by means of semi-automatic biopsy instrument (Sprig-Cut). After having compared different approaches, two experimental protocols have been selected to have the highest number or intact cells: enzimatic digestion with trypsin and explantation.


CONCLUSIONS
Primary cell culture free of microbic contamination, obtained mainly by means of Spring-Cut methods, underwent immunohistochemical analysis to evaluate what kind of cell have been grown in vitro by measuring the expression of CK20 and GFAP both resulted positive. The possibility of setting a primary cell culture which represents the cancer of each patient allows a pharmacologic and biomolecular study which can contribute to the development of a tailored adjuvant therapy with many advantages for the patient in terms of positive answer to the treatment and reduced toxicity.
Spisni
Roberto
R
Dipartimento di Chirurgia, Università di Pisa, Italia. r.spisni@dc.med.unipi.it
Failli
Alessandra
A
Orsini
Giulia
G
Kastsiuchenka
Olga
O
Natale
Gianfranco
G
Castagna
Maura
M
Legitimo
Annalisa
A
Aghasbabyan
Alekandr
A
Ambrosini
Carlo Enrico
CE
Consolini
Rita
R
Miccoli
Paolo
P
D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured