Age-related changes in HSP25 expression in basal ganglia and cortex of F344/BN rats.
Normal aging is associated with chronic oxidative stress. In the basal ganglia, oxidative stress may contribute to the increased risk of Parkinson's disease in the elderly. Neurons are thought to actively utilize compensatory defense mechanisms, such as heat shock proteins (HSPs), to protect from persisting stress. Despite their protective role, little is known about HSP expression in the aging basal ganglia. The purpose of this study was to examine HSP expression in striatum, substantia nigra, globus pallidus and cortex in 6-, 18- and 30-month-old Fischer 344/Brown Norway rats. We found robust age-related increases in phosphorylated and total HSP25 in each brain region studied. Conversely, HSP72 (the inducible form of HSP70) was reduced with age, but only in the striatum. p38 MAPK, a protein implicated in activating HSP25, did not change with age, nor did HSC70 (the constitutive form of HSP70), or HSP60. These results suggest that HSP25 is especially responsive to age-related stress in the basal ganglia.
Gupte
Anisha A
AA
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
Morris
Jill K
JK
Zhang
Hongyu
H
Bomhoff
Gregory L
GL
Geiger
Paige C
PC
Stanford
John A
JA
D000367:Age Factors; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis*; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis; D010766:Phosphorylation; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis
D055551:HSP27 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D048051:p38 Mitogen-Activated Protein Kinases