Broad-host-range vectors for protein expression across gram negative hosts.
Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad-host-range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad-host-range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad-host-range pBBR1 replicon as well as the arabinose-inducible P(BAD) promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli, Pseudomonas putida, and Burkholderia cepacia.
Prior
Jamie E
JE
Department of Chemical and Biological Engineering, University of Colorado, ECCH 111/UCB 424, Boulder, CO 80309, USA.
Lynch
Michael D
MD
Gill
Ryan T
RT
D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005822:Genetic Vectors / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism*
D011994:Recombinant Proteins