Inhibition of the expression on MMP-2, 9 and morphological changes via human fibrosarcoma cell line by 6,6'-bieckol from marine alga Ecklonia cava.
Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-kappaB signal pathway. [BMB reports 2010; 43(1): 62-68].
Zhang
Chen
C
Department of Chemistry, Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
Li
Yong
Y
Shi
Xiujuan
X
Kim
Se-kwon
SK
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement; D004147:Dioxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015536:Down-Regulation; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D015398:Signal Transduction
D000970:Antineoplastic Agents; D004147:Dioxins; D016328:NF-kappa B; C062360:6,6'-bieckol; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9