Fourier transform infrared analysis of the thermal modification of human cornea tissue during conductive keratoplasty.
This paper presents a study using in vitro Fourier transform infrared spectroscopy (FT-IR) analysis to determine the thermal damage induced to the human cornea by the conductive keratoplasty (CK) procedure. Human cornea tissues were treated with CK at different radiofrequency power (58-64%) and pulse duration (0.6-1.0 s) settings. The cornea tissues were cryo-sectioned and FT-IR analysis was performed to detect the extent of thermal damage by the second-derivative analysis of the infrared (IR) spectral bands corresponding to protein secondary structure. The protein amide I and II spectral bands measured in vitro mainly arose from collagen. The denatured cornea tissue showed a higher beta-sheet content than the native tissue. The extent of the thermal damage created by the CK treatment depended on power and duration settings, with the latter having a stronger effect. With clinical settings (60%, 0.6 s), the thermal damage area was confined within a radius of 100 microm. CK treatment duration had a more significant effect on the damage zone than the power setting.
Mechanical Engineering Department, University of Minnesota, Minneapolis 55455, USA.
Li
L
Zhang
Alptekin
A
Aksan
D000328:Adult; D000368:Aged; D001718:Birefringence; D003094:Collagen / Q000032:analysis*; D003315:Cornea / Q000737:chemistry* / Q000601:surgery; D016039:Corneal Transplantation / Q000009:adverse effects* / Q000379:methods; D004564:Electrocoagulation / Q000009:adverse effects* / Q000379:methods; D004867:Equipment Design; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D011489:Protein Denaturation; D011846:Radio Waves / Q000009:adverse effects*; D000078702:Radiofrequency Therapy; D017550:Spectroscopy, Fourier Transform Infrared; D041623:Tomography, Optical Coherence
D005136:Eye Proteins; D003094:Collagen