Propyl gallate inhibits the growth of calf pulmonary arterial endothelial cells via glutathione depletion.
Propyl gallate (PG) as a synthetic antioxidant exerts a variety of effects on tissue and cell functions. Here, we evaluated the effects of PG on the growth and death of endothelial cells (ECs), especially calf pulmonary artery endothelial cells (CPAEC) in relation to reactive oxygen species (ROS) and glutathione (GSH). PG dose-dependently inhibited the growth of CPAEC and human umbilical vein endothelial cells (HUVEC) at 24h. PG induced cell death in CPAEC, which was accompanied by the loss of mitochondrial membrane potential (MMP; DeltaPsi(m)). PG generally increased ROS level in CPAEC but not in HUVEC. PG also dose-dependently increased GSH depleted cells in both ECs. The treatment with antioxidant of N-acetyl-cysteine (NAC) or ascorbate acid (AA) prevented CPAEC growth inhibition and death by PG, which was accompanied by the attenuation of GSH depletion but not by the reduction of ROS level. In conclusion, PG induced growth inhibition and death of ECs, especially CPAEC via GSH depletion.
Department of Physiology, Medical School, Institute for Medical Sciences, Chonbuk National University, JeonJu 561-180, Republic of Korea.
Yong Hwan
YH
Han
Hwa Jin
HJ
Moon
Bo Ra
BR
You
Woo Hyun
WH
Park
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D006131:Growth Inhibitors / Q000633:toxicity*; D006801:Humans; D011435:Propyl Gallate / Q000633:toxicity*; D011651:Pulmonary Artery / Q000166:cytology; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D006131:Growth Inhibitors; D017382:Reactive Oxygen Species; D011435:Propyl Gallate; D005978:Glutathione; D001205:Ascorbic Acid; D000111:Acetylcysteine