An autoradiographic study of cochlea and organs of guinea pigs after intraperitoneal injection of 125I-labelled bFGF.
CONCLUSIONS
The results of our research indicate that 125I-labelled basic fibroblast growth factor (125I-bFGF) has some difficulty in getting across the blood-labyrinth barrier (BLB). Therefore, it is necessary to carry out further study on the route of bFGF administration.


OBJECTIVE
To observe whether bFGF could pass through the BLB after intraperitoneal injection and to establish an experimental basis for its clinical applications.


METHODS
Thirty guinea pigs were divided into three equal groups. Animals in group 1 were administered 125I-bFGF, while animals in groups 2 and 3 were given 125I or saline solution, respectively, via intraperitoneal injection. The animals were sacrificed 2 h later and samples of blood, brain, cochlea, thyroid gland, heart, liver, and kidney were collected and weighed. A radioimmunoassay analyzer was employed to count the CPM values of each sample, and autoradiography was performed on section samples of both cochleae.


RESULTS
The CPM value of organ samples in the 125I group was higher than that in other groups, and radioactive grain was observed in cochlear samples of this group. In the 125I-bFGF group, blood demonstrated the highest CPM value, while cochlea and brain demonstrated the lowest CPM value, with no radioactive grain observed in cochlear samples.
Institute of Otolaryngology, PLA General Hospital, Beijing, PR China. zhaisq@301hospital.com.cn
Suoqiang
S
Zhai
Panzao
P
Chen
Weiwei
W
Guo
Ning
N
Yu
Jianhe
J
Sun
Yunyan
Y
Hu
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology; D002199:Capillary Permeability / Q000502:physiology*; D003051:Cochlea / Q000000981:diagnostic imaging* / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000378:metabolism*; D006168:Guinea Pigs; D007274:Injections, Intraperitoneal; D007457:Iodine Radioisotopes / Q000008:administration & dosage / Q000378:metabolism*; D007668:Kidney / Q000000981:diagnostic imaging / Q000473:pathology; D008099:Liver / Q000000981:diagnostic imaging / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D011863:Radioimmunoassay; D011877:Radionuclide Imaging; D013961:Thyroid Gland / Q000000981:diagnostic imaging / Q000473:pathology; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D016222:Fibroblast Growth Factor 2