Detection of resistance genes and susceptibility patterns in Bacteroides and Parabacteroides strains.
Susceptibility to five antimicrobials was determined for Bacteroides spp. (n = 52) and Parabacteroides distasonis (n = 8). All isolates were susceptible to metronidazole. The resistance rates to ampicillin, cefoxitin, tetracycline and clindamycin were 98%, 9.6%, 65.3% and 19.2% of the Bacteroides strains, respectively. The genes cepA, cfiA, cfxA, tetQ, ermF and nim were found in 69.2%, 17.3% 9.6%, 50%, 7.7% and 3.8% for these strains respectively. All P. distasonis strains were resistant to ampicilin. Cefoxitin, tetracycline and clindamycin resistance rates were 75%, 87.5% and 50%, respectively. The ermF and nim genes were absent and 37.5%, 12.5%, 12.5% and 87.5% of this strains possessed cepA, cfiA, cfxA and tetQ genes, respectively. Ten cfiA gene positive strains of Bacteroides and Parabacteroides were submitted to E-test with imipenem and amoxicillin-clavulanate. The resistance rate to imipenem was 4.1% and 8.3% to amoxicillin-clavulanate. This feature is for the first time described in Brazil.
Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes/UFRJ, Laboratório de Biologia de Anaeróbios, Centro de Ciências da Saúde, Ilha do Fundão, Cidade Universitária, Rio de Janeiro/RJ 21941-902, Brasil. renataboente@yahoo.com.br
Renata F
RF
Boente
Livia Q
LQ
Ferreira
Laís S
LS
Falcão
Karla R
KR
Miranda
Priscilla L S
PL
Guimarães
Joaquim
J
Santos-Filho
Jessica M B D
JM
Vieira
David E
DE
Barroso
Jean-Philippe
JP
Emond
Eliane O
EO
Ferreira
Geraldo R
GR
Paula
Regina M C P
RM
Domingues
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001439:Bacteroides / Q000187:drug effects* / Q000235:genetics*; D001442:Bacteroides Infections / Q000382:microbiology*; D001938:Brazil; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D008795:Metronidazole