In vitro model of cartilage degradation.
This 14-day model of cartilage breakdown involves stimulation of bovine nasal cartilage with a combination of interleukin-1 and oncostatin M. Media is harvested on days 7 and 14 and the conditioned media and remaining cartilage at day 14 assayed for the levels of proteoglycan and collagen fragments using biochemical assays.
Hui
Wang
W
Musculoskeletal Research Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Cawston
Tim E
TE
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D004210:Dissection; D008954:Models, Biological; D011509:Proteoglycans / Q000378:metabolism; D046509:Tissue Culture Techniques
D011509:Proteoglycans; D003094:Collagen