The chloride ion is an environmental factor affecting the biosynthesis of pyoluteorin and 2,4-diacetylphloroglucinol in Pseudomonas sp. YGJ3.
The effects of Cl(-) on the antibiotics productivity of Pseudomonas sp. YGJ3 were investigated. YGJ3 produced pyoluteorin and 2,4-diacetylphloroglucinol, depending on the concentration of Cl(-) in the culture medium. Cl(-) stimulated the biosynthesis of pyoluteorin, thereby repressing 2,4-diacetylphloroglucinol biosynthesis. The cell-free extract from the bacteria grown without Cl(-) showed high activity of monoacetylphloroglucinol acetyltransferase, an essential enzyme in 2,4-diacetylphloroglucinol biosynthesis.
Matano
Ikue
I
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan.
Tsunekawa
Masahiro
M
Shimizu
Syotarou
S
Tanaka
Ichiki
I
Mitsukura
Kouichi
K
Maruyama
Kiyofumi
K
D000900:Anti-Bacterial Agents / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D007477:Ions / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002712:Chlorides; D003470:Culture Media; D007477:Ions; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol