[Lung adenocarcinoma stem cell phenotypes and their correlation with patient prognosis].
OBJECTIVE
To detect the cancer stem cells and to evaluate their prognostic implication in patients with lung adenocarcinoma.


METHODS
Three phenotypic markers of cancer stem cells (SP-C, CCSP and OCT4) in lung adenocarcinoma were detected by immunofluorecence staining. The correlation among the clinicopathological parameters and phenotypes of cancer stem cells as well as survival were analyzed by Cox proportional hazard method.


RESULTS
Of the 57 cases, cancer stem cells were detected in 52, including OCT4(+) bronchioloalveolar stem cell (BASC) phenotype (SP-C(+) CCSP(+) OCT4(+)) in 40 cases and OCT4(-) BASC phenotype (SP-C(+) CCSP(+) OCT4(-)) in 12 cases. Statistical analysis revealed that the phenotype of cancer stem cells was related with the cellular differentiation, i.e. the OCT4(+) BASC phenotype occurred more frequently in the well-differentiated tumors, while the OCT4(-) BASC phenotype usually presented in most of the poorly-differentiated ones. Cox analysis showed that the OCT4(+) BASC phenotype was one of prognostic factors.


CONCLUSIONS
The lung adenocarcinoma stem cells have phenotypic features of bronchioalveolar stem cells (SP-C(+) CCSP(+)). The expression of self-renewal regulatory gene OCT4 in these cells indicates an aggressive nature and unfavorable prognosis.
Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
Xue-yan
XY
Zhang
Bi-qiang
BQ
Zheng
Bao-hui
BH
Han
Jin-su
JS
Huang
Qin
Q
Geng
Hui-li
HL
Xu
Jin
J
Zhou
Qiang-gang
QG
Dong
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D016016:Proportional Hazards Models; D037721:Pulmonary Surfactant-Associated Protein C / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D014598:Uteroglobin / Q000235:genetics / Q000378:metabolism
D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D037721:Pulmonary Surfactant-Associated Protein C; C556389:SCGB1A1 protein, human; C489679:SFTPC protein, human; D014598:Uteroglobin