Effect of contact with titanium alloys on the proliferation of mouse osteoblastic cells in culture.
This study was aimed at studying the effect of contact with titanium alloy plates of different surface textures on the proliferative capability of mouse osteoblastic MC3T3-E1 cells. First, the proliferation characteristics of MC3T3-E1 cells were investigated. MC3T3-E1 cells showed a high capacity for proliferation and survived for a long period even under nutritionally starved conditions. During logarithmic cell growth, the consumption of Ser, Gln, Val, Ile and Leu increased time-dependently. Contact with an hydoxyapatite (HA)-coated titanium alloy plate resulted in the increase in the recovery of cells from the plate by trypsin, and an increase in the consumption of these amino acids, suggesting enhanced cell proliferation. On the contrary, contact with the sandblasted and anodized titanium alloy plates resulted in the reduction of the recovery of the cells from the plate, but a slight increase in the amino acid consumption, suggesting the tight adhesion of the cells to the plates. This study demonstrates that the present method, based on the amino acid consumption of the cells, is useful for monitoring the cell proliferative capability, without detachment of the cells from the plate. This method may be applicable to the study of the interaction between cells and metal plates.
Onuki
Hiroyuki
H
Division of Oral Maxillofacial Surgery, Department of Diagnostic and Therapeutic Sciences, Sakado, Saitama 350-0283, Japan.
Sakagami
Hiroshi
H
Kobayashi
Masahiko
M
Hibino
Yasushi
Y
Yokote
Yoshiko
Y
Nakajima
Hiroshi
H
Shimada
Jun
J
D000497:Alloys / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
D000497:Alloys; D000596:Amino Acids; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium