The prophylactic effects of platelet-leucocyte gel in osteomyelitis: an experimental study in a rabbit model.
Platelet-leucocyte gel (PLG), a new biotechnological blood product, has hitherto been used primarily to treat chronic ulcers and to promote soft-tissue and bone regeneration in a wide range of medical fields. In this study, the antimicrobial efficacy of PLG against Staphylococcus aureus (ATCC 25923) was investigated in a rabbit model of osteomyelitis. Autologous PLG was injected into the tibial canal after inoculation with Staph. aureus. The prophylactic efficacy of PLG was evaluated by microbiological, radiological and histological examination. Animal groups included a treatment group that received systemic cefazolin and a control group that received no treatment. Treatment with PLG or cefazolin significantly reduced radiological and histological severity scores compared to the control group. This result was confirmed by a significant reduction in the infection rate and the number of viable bacteria. Although not comparable to cefazolin, PLG exhibited antimicrobial efficacy in vivo and therefore represents a novel strategy to prevent bone infection in humans.
Jia
W-T
WT
Department of Orthopaedic Surgery, Shanghai Jiaotong University, 200233 Shanghai, People's Republic of China.
Zhang
C-Q
CQ
Wang
J-Q
JQ
Feng
Y
Y
Ai
Z-S
ZS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002437:Cefazolin / Q000627:therapeutic use; D002908:Chronic Disease; D004195:Disease Models, Animal; D005782:Gels; D017708:Leukocyte Transfusion / Q000379:methods*; D010019:Osteomyelitis / Q000000981:diagnostic imaging / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D017713:Platelet Transfusion / Q000379:methods*; D011817:Rabbits; D011859:Radiography; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control*; D013977:Tibia / Q000000981:diagnostic imaging / Q000382:microbiology / Q000473:pathology
D000900:Anti-Bacterial Agents; D005782:Gels; D002437:Cefazolin