Bicyclic compounds repress membrane vesicle production and Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa.
Pseudomonas aeruginosa secretes membrane vesicles (MVs) that deliver several virulence factors as a cargo. We found that indole and its derivative compounds, including 4-hydroxyindole, 5-hydroxyindole, 6-hydroxyindole and isatin, repress MV production significantly. These compounds also repressed the synthesis of Pseudomonas quinolone signal (PQS), which is one of the quorum-sensing signals that upregulate virulence gene expression and positively control MV production. Moreover, we showed that other bicyclic compounds, including 1-naphthol, 2-naphthol, 2,3-dihydroxynaphthalene, 1-aminonaphthalene and 8-quinolinol, significantly repress MV production and PQS synthesis. In conclusion, we provide new information about the chemical structures that inhibit P. aeruginosa virulence.
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Yosuke
Y
Tashiro
Masanori
M
Toyofuku
Toshiaki
T
Nakajima-Kambe
Hiroo
H
Uchiyama
Nobuhiko
N
Nomura
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D015363:Quinolones / Q000037:antagonists & inhibitors*; D022142:Secretory Vesicles / Q000187:drug effects* / Q000378:metabolism
C407944:2-heptyl-3-hydroxy-4-quinolone; D000900:Anti-Bacterial Agents; D001643:Bridged Bicyclo Compounds; D015363:Quinolones