Effect of transport temperature on the Scantibodies total intact parathyroid hormone immunoradiometric assay using the RIAMAT-280.
BACKGROUND
Inadequate temperature affects the stability of intact parathyroid hormone (i-PTH) kits. Room temperature during transport modifies i-PTH results.


METHODS
Percent bound (%B/Bmax) and concentrations (pmol/L) of standards, controls (C1, C2) and pool from eight standard curves were divided into: group I (three curves from kits kept at room temperature for more than 48 h) and group II (five curves from kits kept at 2-8 degrees C) during transport. i-PTH was measured using Scantibodies total i-PTH assay with RIAMAT-280.


RESULTS
%B/Bmax for standards, C1 and C2 were significantly higher in group I versus II (P = 0.04). %B/Bmax for the pool were significantly lower in group I (P = 0.001). i-PTH pool concentration in group I was 51% lower (95% confidence interval, 47-53%, P = 0.001); differences were not significant for C1 (P = 0.25) and C2 (P = 0.57) in both groups.


CONCLUSIONS
Room temperature on i-PTH kit during transport alters the standard curve, resulting in a decrease in i-PTH. Using a pool as internal quality control allows the detection of these changes not detected by kit controls.
Plancha-Mansanet
Ma Carmen
MC
Servicio de Medicina Nuclear, Hospital Universitario Dr Peset, Valencia, Spain. plancha_mca@gva.es
Félix-Fontestad
Jesús
J
Caballero-Calabuig
Elisa
E
Reyes-Ojeda
Ma Dolores
MD
Abreu-Sánchez
Pedro
P
Sopena-Novales
Pablo
P
Martínez-Sanchís
Begoña
B
D001681:Biological Assay / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods; D019411:Clinical Laboratory Techniques; D015592:Immunoradiometric Assay / Q000379:methods; D010281:Parathyroid Hormone / Q000032:analysis*; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
D010281:Parathyroid Hormone; D011933:Reagent Kits, Diagnostic