[Correlation between mTOR signaling transduction pathway and arsenic trioxide response].
This study was aimed to investigate the correlation between mTOR signaling transduction pathway and arsenic trioxide (ATO) effect. The expressions of pmTOR, pAKT and pP70S6K in K562/DNR treated with ATO for different time were detected by Western blot. The apoptosis rate of K562/DNR treated by ATO combined with LY294002 or rapamycin for 120 hours was assayed by flow cytometry. The results showed that the expression of pmTOR in K562/DNR cells treated with ATO for 60 minutes or 120 minutes was higher than that in the control group (p < 0.01); the expressions of pAKT in the cells treated with ATO for 30 minutes or 60 minutes were higher than that in the control group (p < 0.01); the expression of pP70S6K in the cells treated with ATO for 60 minutes was higher than that in the control group (p < 0.01). The apoptosis rate of K562/DNR cells treated with combination of ATO and LY294002 or rapamycin were higher than that in the control group. It is concluded that the mTOR signaling pathway in K562/DNR cells is activated by a certain concentration of ATO, and mTOR signaling pathway inhibitors enhance ATO to trigger apoptosis in K562/DNR cells.
Jiang
Fei
F
Department of Hematology, Shengjing Hospital, China Medical Univercity, Shenyang 110004, Liaoning Province, China.
Liu
Zhuo-Gang
ZG
D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D010087:Oxides / Q000494:pharmacology*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
D001152:Arsenicals; D010087:Oxides; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D000077237:Arsenic Trioxide