A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009.
A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus.
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science, Xiamen University, 422 South Siming Rd., Xiamen, China.
Yixin
Y
Chen
Feihai
F
Xu
Xun
X
Gui
Kunyu
K
Yang
Xingrong
X
Wu
Qingbing
Q
Zheng
Shengxiang
S
Ge
Quan
Q
Yuan
Anthony E T
AE
Yeo
Jun
J
Zhang
Yi
Y
Guan
Honglin
H
Chen
Ningshao
N
Xia
D000818:Animals; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D011933:Reagent Kits, Diagnostic