Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1.
ATP-dependent proteases are currently emerging as key regulators of mitochondrial functions. Among these proteolytic systems, Pim1, a Lon-like serine protease in Saccharomyces cerevisiae, is involved in the control of selective protein turnover in the mitochondrial matrix. In the absence of Pim1, yeast cells have been shown to accumulate electron-dense inclusion bodies in the matrix space, to lose integrity of mitochondrial genome, and to be respiration-deficient. Because of the severity of phenotypes associated with the depletion of Pim1, this protease appears to be an essential component of the protein quality control machinery in mitochondria and to exert crucial functions during the biogenesis of this organelle. Nevertheless, its physiological substrates and partners are not fully characterized. Therefore, we used the combination of different proteomic techniques to assess the nature of oxidized protein substrates and physiological partners of Pim1 protease under non-repressing growth conditions. The results presented here supply evidence that Pim1-mediated proteolysis is required for elimination of oxidatively damaged proteins in mitochondria.
Laboratoire de Biologie Cellulaire du Vieillissement, UR4, Vieillissement, Stress et Inflammation, Université Pierre et Marie Curie-Paris 6, Case Courrier 256, Batiment A, 5ème Etage, 7 Quai Saint Bernard, 75252 Paris Cedex 05, France.
Aurélien
A
Bayot
Monique
M
Gareil
Adelina
A
Rogowska-Wrzesinska
Peter
P
Roepstorff
Bertrand
B
Friguet
Anne-Laure
AL
Bulteau
D049069:ATP-Dependent Proteases / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D049070:Protease La / Q000378:metabolism*; D011489:Protein Denaturation; D017510:Protein Folding; D025941:Protein Interaction Mapping; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D013379:Substrate Specificity
D024101:Mitochondrial Proteins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D010447:Peptide Hydrolases; D049069:ATP-Dependent Proteases; C085155:PIM1 protein, S cerevisiae; D012697:Serine Endopeptidases; D049070:Protease La; D010100:Oxygen