Maxillary sinus floor elevation using a tissue engineered bone complex with BMP-2 gene modified bMSCs and a novel porous ceramic scaffold in rabbits.
OBJECTIVE
To study the effects of maxillary sinus floor elevation by a tissue engineered bone complex with bone morphogenetic protein-2 (BMP-2) gene modified bone marrow stromal cells (bMSCs) and a novel porous ceramic scaffold (OsteoBone) in rabbits.


METHODS
bMSCs derived from New Zealand rabbit bone marrow were cultured and transduced with adenovirus AdBMP-2 and with AdEGFP gene (without BMP-2 gene sequence) as a control, respectively, in vitro. These bMSCs were then combined with OsteoBone scaffold at a concentration of 2 x 10(7)cells/ml and used to elevate the maxillary sinus floor in rabbits. Eight rabbits were randomly allocated into groups and sacrificed at weeks 2 and 4. For each time point, 8 maxillary sinus floor elevation surgeries were made bilaterally in 4 rabbits for the two groups (n=4 per group): group A (AdBMP-2-bMSCs/material) and group B (AdEGFP-bMSCs/material). All samples were evaluated by histologic and histomorphometric analysis.


RESULTS
The augmented maxillary sinus height was maintained for both groups over the entire experimental period, while new bone area increased over time for group A. At week 4 after operation, bone area in group A was significantly more than that in group B (P<0.05), and was more obviously detected in the center of the elevated space. Under a confocal microscope, green fluorescence in newly formed bone was observed in the EGFP group, which suggests that those implanted bMSCs had contributed to the new bone formation.


CONCLUSIONS
bMSCs modified with AdBMP-2 gene can promote new bone formation in elevating the rabbit maxillary sinus. OsteoBone scaffold could be an ideal carrier for gene enhanced bone tissue engineering.
Sun
X-Juan
XJ
Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. ms_sunxiaojuan@126.com
Xia
L-Guo
LG
Chou
L Lee
LL
Zhong
Wei
W
Zhang
X-Li
XL
Wang
S-Yi
SY
Zhao
Jun
J
Jiang
X-Quan
XQ
Zhang
Z-Yuan
ZY
D000256:Adenoviridae / Q000235:genetics; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002516:Ceramics / Q000737:chemistry; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D049408:Luminescent Agents; D008297:Male; D008437:Maxilla / Q000473:pathology / Q000601:surgery; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery*; D018613:Microscopy, Confocal; D023421:Models, Animal; D010012:Osteogenesis / Q000502:physiology; D010027:Osteotomy / Q000379:methods; D011817:Rabbits; D011897:Random Allocation; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014161:Transduction, Genetic
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D049408:Luminescent Agents; D049452:Green Fluorescent Proteins