Transient up-regulation of immunity- and apoptosis-related genes in Caco-2 cells cocultured with THP-1 cells evaluated by DNA microarray analysis.
We analyzed changes in gene expression in Caco-2 cells cocultured with THP-1 cells over time periods of 0, 1, 3, 6, 24, and 48 h using a DNA microarray. Differentially expressed genes extracted with maSigPro indicated that an early defense response and cell death occured at the same time, causing an inflammatory condition.
Ishimoto
Yoko
Y
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. sabuyoko43@hotmail.com
Nakai
Yuji
Y
Satsu
Hideo
H
Totsuka
Mamoru
M
Shimizu
Makoto
M
D017209:Apoptosis / Q000235:genetics*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004247:DNA / Q000235:genetics*; D006801:Humans; D007109:Immunity / Q000235:genetics*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015854:Up-Regulation / Q000235:genetics
D004247:DNA