[Antagonistic effect of microwave on hematopoietic damage of mice induced by gamma-ray irradiation].
OBJECTIVE
To investigate antagonistic effect of microwave on hematopoietic damage of mice induced by gamma-ray irradiation.


METHODS
Male healthy Kunning mice were treated with low dose microwave radiation before exposure to (60)Co gamma-ray irradiation of 8.0 Gy. The 30-day survival rate and average survival time of the mice after the treatment were examined. Peripheral blood parameters and the organ indexes of thymus and spleen were also observed in the irradiated mice. After exposure to 5.0 Gy gamma irradiation, indexes of hematopoietic foci formation of bone marrow cells (CFU-GM) and the proliferation activity of BMNCs were examined. The serum concentration of hemopoietic factors (GM-CSF and IL-3) were detected by ELISA kits.


RESULTS
Pre-exposure with 120 microW/cm(2) 900 MHz microwave increased the 30-day survival rate (P < 0.05) and the number of white blood cells of gamma-ray treated mice. The increases of the organ indexes of thymus and spleen, proliferation activity of BMNCs and CFU-GM hematopoietic foci numbers, as well as the higher serum concentration of GM-CSF and IL-3 were observed in the microwave pre-exposure group.


CONCLUSIONS
Low dose microwave radiation may exert potential antagonistic effects on hematopoietic injuries induced by ionizing radiation. The underlying mechanisms might be related with stimulation of hematopoietic growth factors expression, promotion of HSCs/HPCs proliferation, suppression on the reduction of HSCs/HPCs caused by (60)Co gamma-ray, and enhanced construction of the hematopoietic system.
Cao
Yi
Y
School of Radiation Medicine and Public Health, Sowchow University, Suzhou 215123, China.
Xu
Qian
Q
Lu
Min-xia
MX
Jin
Zong-da
ZD
Du
Hou-bing
HB
Li
Jian-xiang
JX
Nie
Ji-hua
JH
Tong
Jian
J
D000818:Animals; D001853:Bone Marrow / Q000473:pathology / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000473:pathology / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D007377:Interleukin-3 / Q000097:blood; D008297:Male; D051379:Mice; D008872:Microwaves; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000473:pathology / Q000517:prevention & control*
D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor