Antrocamphin A, an anti-inflammatory principal from the fruiting body of Taiwanofungus camphoratus , and its mechanisms.
The fungus Taiwanofungus camphoratus is commonly used for medicinal purposes in Taiwan. It is used as a detoxicant for food poisoning and considered to be a precious folk medicine for hepatoprotection and anti-inflammation. In this study, a lipopolysaccaride (LPS)-challenged ICR mouse acute inflammation model and a LPS-induced macrophage model were used to evaluate the anti-inflammatory activity of T. camphoratus. Ethanol extract of T. camphoratus significantly inhibited expression of iNOS and COX-2 in the liver of LPS-challenged acute inflammatory mice. The ethyl acetate fraction and its isolated compound, antrocamphin A, significantly suppressed nitrite/nitrate concentration in LPS-challenged RAW 264.7 cells. Antrocamphin A showed potent anti-inflammatory activity by suppressing pro-inflammatory molecule release via the down-regulation of iNOS and COX-2 expression through the NF-kappaB pathway. This study, therefore, first demonstrates the bioactive compound of T. camphoratus and illustrates the mechanism by which it confers its anti-inflammatory activity.
Hsieh
Yu-Hsin
YH
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
Chu
Fang-Hua
FH
Wang
Ya-Shin
YS
Chien
Shih-Chang
SC
Chang
Shang-Tzen
ST
Shaw
Jei-Fu
JF
Chen
Chieh-Yin
CY
Hsiao
Wen-Wei
WW
Kuo
Yueh-Hsiung
YH
Wang
Sheng-Yang
SY
D000480:Alkynes / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001483:Base Sequence; D001487:Basidiomycota / Q000737:chemistry*; D017931:DNA Primers; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000480:Alkynes; D000840:Anisoles; D000893:Anti-Inflammatory Agents; D017931:DNA Primers; C550852:antrocamphin A