Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.
OBJECTIVE
CD55 (decay-accelerating factor) is best known for its role in the negative regulation of the complement system. Indeed, lack of this molecule leads to disease aggravation in many autoimmune disease models. However, CD55 is abundantly present on fibroblast-like synoviocytes and is also a ligand of the adhesion-class heptahelical receptor CD97, which is expressed by infiltrating macrophages. Treatment with antibodies to CD97 ameliorates the collagen-induced model of rheumatoid arthritis (RA) in DBA/1 mice, but the net contribution of CD55 is unknown. This study was undertaken to investigate the role of CD55 in experimental RA.


METHODS
Arthritis was induced in wild-type, CD55(-/-), and CD97(-/-) mice using collagen-induced and K/BxN serum-transfer models. Incidence of arthritis was monitored over time, and disease activity was assessed by clinical and immunohistochemical evaluation.


RESULTS
In contrast to observations in many inflammatory disease models, lack of CD55 resulted in decreased arthritis in experimental models of RA. Consistent with the previously reported effects of anti-CD97 antibody treatment, CD97(-/-) mice had reduced arthritis activity compared with wild-type controls.


CONCLUSIONS
Our findings indicate that the lack of CD55 or CD97 in 2 different models of arthritis increases resistance to the disease. These findings provide insight into a role for CD55 interaction with CD97 in the pathogenesis of RA and suggest that therapeutic strategies that disrupt CD55/CD97 may be clinically beneficial.
Hoek
Robert M
RM
Academic Medical Center, Amsterdam, The Netherlands. r.hoek@nin.knaw.nl.
de Launay
Daphne
D
Kop
Else N
EN
Yilmaz-Elis
A Seda
AS
Lin
Feng
F
Reedquist
Kris A
KA
Verbeek
J Sjef
JS
Medof
M Edward
ME
Tak
Paul P
PP
Hamann
Jörg
J
D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics / Q000517:prevention & control*; D018958:CD55 Antigens / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D017353:Gene Deletion; D006801:Humans; D007596:Joints / Q000276:immunology / Q000473:pathology; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D051381:Rats; D043562:Receptors, G-Protein-Coupled
C490633:Adgre5 protein, mouse; D018958:CD55 Antigens; D008562:Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled