Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label.
A sensitive and selective method for the determination of 17beta-oestradiol by fluorescence immunoassay (FIA) was established on the basis of quantum dots (QDs) as label. The complex of biotin-labelled anti-rabbit IgG and strepavidin conjugated by quantum dots (QD-SA) was regarded as a probe in this system and the strepavidin-biotin system as signal amplification system. After optimising the conditions of the immunoreaction, such as the concentration of the reagent and the pH of the buffer solution, the linear range and the limit of detection of 17beta-oestradiol were 0.01-10,000ngml(-1) and 0.00542ngml(-1), respectively. This method was applied to determine oestradiol in water samples, with the percent recoveries in the range of 86-113%.
Sun
Meimei
M
College of Chemistry and Chemical Engineering, Liaocheng University, No. 1 Hunan Road, Liaocheng, Shandong 252059, PR China.
Du
Lingyun
L
Gao
Suqin
S
Bao
Yuhong
Y
Wang
Shuhao
S
D000818:Animals; D004958:Estradiol / Q000032:analysis*; D005455:Fluorescent Antibody Technique / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D045663:Quantum Dots; D011817:Rabbits; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000032:analysis
D005456:Fluorescent Dyes; D014867:Water; D004958:Estradiol; D019809:Streptavidin