Design of double stimuli-responsive polyelectrolyte microcontainers for protein soft encapsulation.
We have designed new double stimuli-responsive polyelectrolyte microcapsules to be useable under physiological conditions to handle biomacromolecules while avoiding the risk of denaturation. They are made of poly(4-vinylpyridine hydrochloride) (PVP) and poly(sodium styrene sulfonate) (PSS). The microcontainers are sensitive to temperature variation, as they irreversibly shrink under heating. In addition, the capsules reversibly swell at pH > 6, making it possible to encapsulate human serum proteins by diffusion through the polymer membrane. Encapsulation efficiency is quantified by fluorescence techniques.
DSV/iBEB/SBTN/LEPC, ICSM, UMR 5257 CEACNRS-UM2-ENSCM, CEA Marcoule, BP 17171, 30207 Bagnols-sur-Ceze cedex, France.
Christian
C
Basset
Christophe
C
Harder
Claude
C
Vidaud
Christophe
C
Déjugnat
D001798:Blood Proteins / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011489:Protein Denaturation; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D004573:Electrolytes