Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis.
L-Amino acid alpha-ligase (Lal), catalyzing the formation of alpha-dipeptides from unprotected L-amino acids in an ATP-dependent manner, is used in cost-effective fermentative production of dipeptides. We searched for novel Lals by in silico screening using Hidden Markov Model-based profile analysis, and identified five novel Lals that showed low similarity and different substrate specificity from known Lals.
Bioprocess Development Center, Kyowa Hakko Bio Co., Ltd., Tsukuba, Ibaraki, Japan. akihiro.senoo@kyowa-kirin.co.jp
Akihiro
A
Senoo
Kazuhiko
K
Tabata
Yoshiyuki
Y
Yonetani
Makoto
M
Yagasaki
D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology; D003198:Computer Simulation; D004151:Dipeptides / Q000096:biosynthesis* / Q000235:genetics; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008390:Markov Chains; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012995:Solubility; D013379:Substrate Specificity / Q000235:genetics
D000596:Amino Acids; D004151:Dipeptides; D009842:Oligopeptides; D011994:Recombinant Proteins; D008025:Ligases