Visualizing "green oil" in live algal cells.
We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content.
Cooper
Mark Scott
MS
Department of Biology, University of Washington, Seattle, WA 98195-1800, USA. mscooper@u.washington.edu
Hardin
William Robert
WR
Petersen
Timothy Wayne
TW
Cattolico
Rose Ann
RA
D001896:Boron Compounds / Q000737:chemistry*; D056890:Eukaryota / Q000737:chemistry / Q000166:cytology*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes