Relocalization of upstream binding factor to viral replication compartments is UL24 independent and follows the onset of herpes simplex virus 1 DNA synthesis.
Herpes simplex virus 1 (HSV-1) induces relocalization of several nucleolar proteins. We have found that, as for fibrillarin, the HSV-1-induced redistribution of two RNA polymerase I components, upstream binding factor (UBF) and RPA194, was independent of the viral protein UL24, which affects nucleolin localization. Nevertheless, the kinetics and sites of redistribution for fibrillarin and UBF differed. Interestingly, UBF remained associated with RPA194 during infection. Although UBF is redistributed to viral replication compartments during infection, we did not detect foci of UBF at early sites of viral DNA synthesis, suggesting that it may not be directly involved in this process at early times.
INRS-Institut Armand-Frappier, Université du Québec, 531 Boulevard des Prairies, Laval, Québec H7V 1B7, Canada.
Maria H
MH
Lymberopoulos
Angela
A
Pearson
D000818:Animals; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D018613:Microscopy, Confocal; D035463:Pol1 Transcription Initiation Complex Proteins / Q000378:metabolism*; D011485:Protein Binding; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D002868:Chromosomal Proteins, Non-Histone; D004279:DNA, Viral; D035463:Pol1 Transcription Initiation Complex Proteins; C086267:UL24 protein, Human herpesvirus 1; D014764:Viral Proteins; C047458:fibrillarin; C063844:transcription factor UBF