A time-course study of gene responses of chicken granulosa cells to Salmonella Enteritidis infection.
Consumption of eggs contaminated with Salmonella Enteritidis (SE) has been recognized as one of the important causes of human foodborne salmonellosis. Chicken granulosa cells (cGCs) comprise the last tissue layer surrounding the yolk in preovulatory follicles and are a preferred site for SE invasion. To understand the cGC response to SE infection, we conducted an in vitro time-course study to identify cGC transcriptional changes using chicken whole genome microarrays. The expression of 135 (4h postinfection) and 120 cGC genes (48 h postinfection) were altered (P<.01) compared to uninfected cells. Many of the altered genes were related to immune response, physiological processes, signal transduction, and transcription. Furthermore, we also found that the Jak-STAT pathway, which is essential in the regulation of cellular cytokines and growth factors, was highly active in this study. Among the genes identified by microarray, the mRNA levels of TLR15, IL-6, CXCLi1, CXCLi2, and K203 were shown to be upregulated by real-time RT-PCR (qRT-PCR). In contrast, the mRNA levels of RASD1 and HB-EGF decreased according to both microarray and qRT-PCR analyses. These results suggest that during the SE infection, cGCs recruit cells of the innate immune responses; the infection may also induce suppression of cGC cell proliferation, which alters follicular development and ovulation.
Tsai
Hsiang-Jung
HJ
Zoonoses Research Center and School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.
Chiu
Chih-Hsien
CH
Wang
Chia-Lan
CL
Chou
Chung-Hsi
CH
D000818:Animals; D002645:Chickens; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006107:Granulosa Cells / Q000382:microbiology* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012477:Salmonella enteritidis; D013997:Time Factors
D012333:RNA, Messenger