Schistosomicidal evaluation of Zanthoxylum naranjillo and its isolated compounds against Schistosoma mansoni adult worms.
Chemical investigation of the EtOAc fraction (EF) obtained from the ethanolic extract of Zanthoxylum naranjillo (Rutaceae) leaves (EE) by preparative HPLC resulted in the isolation of protocatechuic acid (1), gallic acid (2), p-hydroxybenzoic acid (3), and 5-O-caffeoylshikimic acid (4). This is the first time that the presence of compounds 1-4 in Z. naranjillo has been reported. Compounds 1-4, the EE, and EF were tested in vitro against Schistosoma mansoni adult worms. The results showed that the S. mansoni daily egg production decreased by 29.8%, 13.5%, 28.4%, 17.7%, 16.3%, and 6.4%, respectively. Compounds 1 and 3 were also able to separate adult worm pairs into male and female. This activity may be correlated with the reduction in egg production, since 1 and 3 showed better inhibitory properties compared with 2 and 4.
Universidade de Franca, Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
Caio G
CG
Braguine
Eveline S
ES
Costa
Lizandra G
LG
Magalhães
Vanderlei
V
Rodrigues
Ademar A
AA
da Silva Filho
Jairo K
JK
Bastos
Márcio L A
ML
Silva
Wilson R
WR
Cunha
Ana H
AH
Januário
Patrícia M
PM
Pauletti
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000469:parasitology; D009043:Motor Activity / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D012556:Schistosomicides / Q000302:isolation & purification* / Q000494:pharmacology; D032106:Zanthoxylum / Q000737:chemistry*
D012556:Schistosomicides