Application of laccase-mediator system (LMS) for the degradation of organophosphorus compounds.
Degradation of organophosphorus compounds was achieved in the presence of purified fungal laccase from Trametes versicolor and a small molecular weight redox mediator (ABTS). This laccase-mediator system (LMS) catalyzed degradation of VX, PhX and VR while had no apparent effect on CVX, ecothiophate or demeton. Inhibition of ABTS oxidation was shown with VX, PhX, VR and CVX. Results with CVX suggest either no degradation subsequent to interaction with the laccase active site or the formation of a new toxic compound. PhX degradation was also monitored by mass spectroscopy, a method that allowed us to identify certain intermediates formed during OP degradation. Altogether, results underline the importance of the OP nitrogen atom at beta-position and of its substituents, even though the intimate mechanism of laccase-catalyzed degradation is not yet known.
Trovaslet-Leroy
Marie
M
Département de Toxicologie, Centre de Recherches du Service de Santé des Armées (CRSSA), 24 Avenue des maquis du Grésivaudan, 38702 La Tronche Cedex, France. mtrovaslet@hotmail.com
Jolivalt
Claude
C
Froment
Marie-Thérèse
MT
Brasme
Bernardin
B
Lefebvre
Bertrand
B
Daveloose
Denis
D
Nachon
Florian
F
Masson
Patrick
P
D001711:Biotransformation; D042845:Laccase / Q000378:metabolism*; D013058:Mass Spectrometry; D009943:Organophosphorus Compounds / Q000378:metabolism* / Q000633:toxicity; D010084:Oxidation-Reduction; D055454:Trametes / Q000201:enzymology
D009943:Organophosphorus Compounds; D042845:Laccase