Acute toxicity testing of chemicals-Opportunities to avoid redundant testing and use alternative approaches.
Assessment of the acute systemic oral, dermal, and inhalation toxicities, skin and eye irritancy, and skin sensitisation potential of chemicals is required under regulatory schemes worldwide. In vivo studies conducted to assess these endpoints can sometimes be associated with substantial adverse effects in the test animals, and their use should always be scientifically justified. It has been argued that while information obtained from such acute tests provides data needed to meet classification and labelling regulations, it is of limited value for hazard and risk assessments. Inconsistent application of in vitro replacements, protocol requirements across regions, and bridging principles also contribute to unnecessary and redundant animal testing. Assessment of data from acute oral and dermal toxicity testing demonstrates that acute dermal testing rarely provides value for hazard assessment purposes when an acute oral study has been conducted. Options to waive requirements for acute oral and inhalation toxicity testing should be employed to avoid unnecessary in vivo studies. In vitro irritation models should receive wider adoption and be used to meet regulatory needs. Global requirements for sensitisation testing need continued harmonisation for both substance and mixture assessments. This paper highlights where alternative approaches or elimination of tests can reduce and refine animal use for acute toxicity requirements.
Creton
Stuart
S
National Centre for the Replacement, Refinement and Reduction of Animals in Research, London, UK. stuart.creton@nc3rs.org.uk
Dewhurst
Ian C
IC
Earl
Lesley K
LK
Gehen
Sean C
SC
Guest
Robert L
RL
Hotchkiss
Jon A
JA
Indans
Ian
I
Woolhiser
Michael R
MR
Billington
Richard
R
D000284:Administration, Oral; D000287:Administration, Topical; D000826:Animal Testing Alternatives / Q000331:legislation & jurisprudence / Q000379:methods*; D000818:Animals; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects; D007391:International Cooperation; D018570:Risk Assessment / Q000331:legislation & jurisprudence / Q000379:methods; D023422:Skin Irritancy Tests / Q000379:methods; D023382:Toxicity Tests, Acute / Q000379:methods*