Evaluation of performance and community dynamics of microorganisms during treatment of distillery spent wash in a three stage bioreactor.
The ability of Emericella nidulans var. lata, Neurospora intermedia and Bacillus sp. to treat distillery spent wash in a three stage bioreactor was investigated. Process parameters were optimized in shake flask cultures with the individual strains before treatment of the effluent in a 15-l bioreactor. Treatment was first carried out by the fungi followed by bacteria. The treated effluent showed significant reduction in color (82%) and COD (93%) after 30 h. Metabolites formed after degradation of complex polymers in distillery effluent were assayed by gas chromatography-mass spectroscopy and included furan, simple acid types and organic compounds. Denaturing gradient gel electrophoresis of 16S rDNA and 18S rDNA sequences amplified from DNA isolated from the reactor communities indicated the presence of other organisms besides those introduced initially. The microbial communities were able to carry out bioremediation of distillery effluent and produce discharge that conforms to safety standards.
Kaushik
Garima
G
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. garima4rinku@rediffmail.com
Gopal
Madhuban
M
Thakur
Indu Shekhar
IS
D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000494:pharmacology; D003116:Color; D057168:Distillation; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000187:drug effects / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000302:isolation & purification; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D002244:Carbon; D009584:Nitrogen; D010100:Oxygen