Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis.
Bovine tuberculosis caused by Mycobacterium bovis is an important zoonosis. In this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. The technique will be of value in the clinical and field-based diagnosis of M. bovis and can differentiate it from other bacteria such as Corynebacterium diphtheriae, Streptococcus pneumoniae, β-haemolytic streptococci, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Staphylococcus aureus, and Klebsiella pneumoniae. The limit of detection was 10 copies/μL and the results were corroborated by PCR. The method was highly specific and more sensitive than PCR.
Zhang
Jun
J
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, People's Republic of China.
Zhang
Gui-Hong
GH
Yang
Lin
L
Huang
Ren
R
Zhang
Yu
Y
Jia
Kun
K
Yuan
Wen
W
Li
Shou-Jun
SJ
D000818:Animals; D002417:Cattle; D005784:Gene Amplification; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000592:standards / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000175:diagnosis*