Chloride channel conductance is required for NGF-induced neurite outgrowth in PC12 cells.
We have previously shown that in PC12 cells: (1) high extracellular KCl induces moesin phosphorylation, an event which was dependent on chloride channel activation, and (2) NGF induces moesin phosphorylation which is required for neurite outgrowth. These results suggest that NGF-induced intracellular signaling and neurite outgrowth is also mediated by activation of anion channels. Using a patch-clamp technique, we found that NGF treatment increased anionic conductance in PC12 cells, an effect which was completely blocked by NPPB, a chloride channel inhibitor. Also, the NGF-induced moesin phosphorylation was suppressed by NPPB. Additionally, NPPB and SITS, another chloride channel blocker, suppressed NGF-induced TrkA phosphorylation and subsequent PI3K/Akt phosphorylation and Rac1 activation in PC12 cells. Moreover, the chloride channel inhibitors also suppressed the neurite outgrowth and decreased the cell viability in response to long-term treatment of NGF. In summary, our results suggest that chloride ion flux plays an important role in TrkA-mediated signaling pathway during NGF-induced differentiation of PC12 cells.
Kim
Soo Yeon
SY
Department of Medical Biotechnology, Dongguk University, Republic of Korea.
Shin
Dong Hun
DH
Kim
Sung Joon
SJ
Koo
Byung-Soo
BS
Bae
Chang-Dae
CD
Park
Joobae
J
Jeon
Songhee
S
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D004594:Electrophysiology; D047468:Immunoprecipitation; D007473:Ion Channels / Q000187:drug effects; D008840:Microfilament Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D009579:Nitrobenzoates / Q000494:pharmacology; D051058:Oncogene Protein v-akt / Q000378:metabolism; D016716:PC12 Cells; D018408:Patch-Clamp Techniques; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D020917:Receptor, trkA / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
D020533:Angiogenesis Inhibitors; D018118:Chloride Channels; D007473:Ion Channels; D008840:Microfilament Proteins; D009414:Nerve Growth Factors; D009579:Nitrobenzoates; C071058:moesin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D019869:Phosphatidylinositol 3-Kinases; D020917:Receptor, trkA; D051058:Oncogene Protein v-akt; D020830:rac1 GTP-Binding Protein; D011188:Potassium