Low-energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha(v) beta(3) integrin in rats.
It has previously been reported that low-energy laser irradiation stimulated the velocity of tooth movement via the receptor activator of nuclear factor kappa B (RANK)/RANK ligand and the macrophage colony-stimulating factor/its receptor (c-Fms) systems. Matrix metalloproteinase (MMP)-9, cathepsin K, and alpha(v) beta(3) [alpha(v)beta3] integrin are essential for osteoclastogenesis; therefore, the present study was designed to examine the effects of low-energy laser irradiation on the expression of MMP-9, cathepsin K, and alpha(v)beta3 integrin during experimental tooth movement. Fifty male, 6-week-old Wistar strain rats were used in the experiment. A total force of 10g was applied to the rat molars to induce tooth movement. A Ga-Al-As diode laser was used to irradiate the area around the moving tooth and, after 7 days, the amount of tooth movement was measured. To determine the amount of tooth movement, plaster models of the maxillae were made using a silicone impression material before (day 0) and after tooth movement (days 1, 2, 3, 4, and 7). The models were scanned using a contact-type three-dimensional (3-D) measurement apparatus. Immunohistochemical staining for MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 was performed. Intergroup comparisons of the average values were conducted with a Mann-Whitney U-test for tooth movement and the number of tartrate-resistant acid phosphatase (TRAP), MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3-positive cells. In the laser-irradiated group, the amount of tooth movement was significantly greater than that in the non-irradiated group at the end of the experiment (P < 0.05). Cells positively stained with TRAP, MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 were found to be significantly increased in the irradiated group on days 2-7 compared with those in the non-irradiated group (P < 0.05). These findings suggest that low-energy laser irradiation facilitates the velocity of tooth movement and MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 expression in rats.
Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan. yamaguchi.masaru@nihon-u.ac.jp
Masaru
M
Yamaguchi
Masami
M
Hayashi
Shouji
S
Fujita
Takamasa
T
Yoshida
Tadahiko
T
Utsunomiya
Hirotsugu
H
Yamamoto
Kazutaka
K
Kasai
D000539:Alveolar Process / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D016723:Bone Remodeling / Q000528:radiation effects*; D056657:Cathepsin K / Q000096:biosynthesis; D003799:Dental Stress Analysis; D039302:Integrin alphaVbeta3 / Q000096:biosynthesis; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D010010:Osteoclasts / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014087:Tooth Movement Techniques
D039302:Integrin alphaVbeta3; D056657:Cathepsin K; D020780:Matrix Metalloproteinase 9