Microchip electrophoresis for monitoring covalent attachment of poly(ethylene glycol) to proteins.
A microchip electrophoretic method was applied to monitor and characterize the covalent attachment of poly(ethylene glycol) (PEGylation) of two proteins, alpha-lactalbumin and bovine serum albumin, using several poly(ethylene glycol) (PEG) derivatives with molecular weights from 1 to 20 kDa. This method effectively separated multi-PEGylated proteins in a size-based manner and allowed monitoring of the PEGylation pattern with the advantages of high speed, minimal sample consumption, and high reproducibility. Microchip electrophoresis would be a very useful tool for protein PEGylation studies such as reaction monitoring, purity checks, and characterization of PEGylated protein products.
Park
Eun Ji
EJ
College of Pharmacy, Kyungsung University, Nam-ku, Busan 608-736, South Korea.
Na
Dong Hee
DH
D000818:Animals; D002417:Cattle; D046209:Electrophoresis, Microchip / Q000379:methods*; D007768:Lactalbumin / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D007768:Lactalbumin