Injection of lentivirus into stage-X blastoderm for the production of transgenic quail.
This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.
Poynter
Greg
G
Division of Biology and Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Huss
David
D
Lansford
Rusty
R
D000818:Animals; D030801:Animals, Genetically Modified; D001756:Blastoderm / Q000166:cytology*; D018929:Cell Culture Techniques; D003370:Coturnix / Q000235:genetics*; D018014:Gene Transfer Techniques; D005854:Germ Cells / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics*; D010063:Ovum / Q000502:physiology*