Production of Xylanase with a transformant of Aspergillus oryzae RIB40 in a Liquid-Surface Immobilization (LSI) System.
Xylanase production by a XynF1 (33 kDa)-transformant of Aspergillus oryzae RIB40 was compared between submerged cultivation (SmC) and liquid-surface immobilization (LSI) systems. While the accumulation of xylanase in the SmC decreased by prolonged incubation, LSI system enabled the continuation of xylanase production to afford 4.5-fold xylanase production compared with the SmC system.
Ozeki
Kenji
K
Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan. ozeki@neptune.kanazawa-it.ac.jp
Takagi
Yoshihiro
Y
Oda
Shinobu
S
Ohashi
Shinichi
S
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*