The effects of selective and nonselective phosphodiesterase inhibitors on phagocytic cells in mice.
Milrinone (1 mg/kg i.m.), sildenafil (1 mg/kg p.o), and aminophylline (20 mg/kg i.m.) were administered to mice once or five times. The drugs increased the production of IL-1beta and NO by peritoneal macrophages. Milrinone or aminophylline did not change the percentage of phagocytosing cells. A single administration of sildenafil increased the percentage of phagocytosing granulocytes (after 12 h). Sildenafil administered five times decreased the percentage of phagocytosing monocytes (72 h after the last dose). A single administration of the drugs did not change the oxidative burst activity. PDE inhibitors administered five times temporarily enhanced the percentage of cells producing reactive oxidants.
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. marianna.szczypka@up.wroc.pl
Marianna
M
Szczypka
Bozena
B
Obmińska-Mrukowicz
D000628:Aminophylline / Q000494:pharmacology*; D000818:Animals; D005260:Female; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D020105:Milrinone / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects; D009569:Nitric Oxide / Q000032:analysis; D010586:Phagocytes / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology*
D053583:Interleukin-1beta; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000628:Aminophylline; D009569:Nitric Oxide; D000068677:Sildenafil Citrate; D020105:Milrinone