Granulocyte colony-stimulating factor enhances bone marrow mononuclear cell homing to the liver in a mouse model of acute hepatic injury.
BACKGROUND
Experiments have reported that granulocyte colony stimulating factor (G-CSF) can mobilize stem cells. However, few studies have examined the effect of G-CSF on bone marrow mononuclear cell (BMMC) mobilization, in particular regarding their capability to home to acutely injured liver.


OBJECTIVE
The aim of this study was to evaluate the effort of G-CSF on BMMC homing to the liver following chemically-induced hepatic failure.


METHODS
BMMC were isolated from mice, pre-labeled with PKH26 and infused into the mice in which hepatic injury had been induced followed by administration of G-CSF or vehicle. Livers were studied by fluorescent microscopy after transplantation of pre-labeled BMMC.


RESULTS
PKH26 labeled cells were found in liver tissue at 102 ± 10 cells/high power field in the BMMC+G-CSF group and 30 ± 5 cells/high power field in the BMMC group, but none in the G-CSF group and the control group (P < 0.05). In the former two groups the majority of PKH26 labeled cells colocalized with proliferative cell nuclear antigen (PCNA). The number of PCNA positive cells in the BMMC+G-CSF group was 20 ± 4 cells/high power field, while in the BMMC group it was 14 ± 2 cells/high power field, in the G-CSF group 12 ± 2 cells/high power field, and 8 ± 1 cells/high power field in the control group. Moreover, albumin expression was increased in the BMMC+G-CSF treated group (149 ± 7/high power field) relative to the BMMC group (48 ± 6/high power field), the G-CSF group (44 ± 5/high power field) and the vehicle group (30 ± 6/high power field), with the former three groups showing elevated levels as compared to vehicle control (30 ± 6) (P < 0.05).


CONCLUSIONS
Transplanted BMMC may home to injured liver, which appears to be enhanced by G-CSF administration.
Department of Gastroenterology, First Hospital, Jilin University, 130021, Changchun, China.
Shi-Zhu
SZ
Jin
Xiang-Wei
XW
Meng
Xun
X
Sun
Ming-Zi
MZ
Han
Bing-Rong
BR
Liu
Xin-Hong
XH
Wang
Li-Ying
LY
Sun
Qi
Q
Huang
Rui-Bo
RB
Zhao
Xiang
X
Ban
Hong-Ying
HY
Yu
Hong-Wei
HW
Yu
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001706:Biopsy; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects; D002477:Cells; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009930:Organic Chemicals
D000418:Albumins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26; D016179:Granulocyte Colony-Stimulating Factor; D002251:Carbon Tetrachloride