Gold nanoparticle-based colorimetric sensor for studying the interactions of beta-amyloid peptide with metallic ions.
In this paper, a kind of beta-amyloid peptide (Abeta1-16) conjugated gold nanoparticles (Abeta1-16@GNPs) are prepared and employed as colorimetric indicator for studying the interaction of beta-amyloid peptide with metallic ions (e.g. Zn(2+) and Ca(2+)). In the presence of Zn(2+), mono-dispersing Abeta1-16@GNPs enable to form aggregates or attach on the SHG-44 (human glioma cell) cellular surface which results in significant color change of the solution. The experimental results indicate that Zn(2+) can interact with Abeta1-16 and form Zn(2+)-beta-amyloid peptide complexes. In particular, in the presence of Zn(2+), a time-dependent interaction of cells with Abeta1-16@GNPs has been observed that may suggest different expression levels of beta-amyloid peptide related proteins in various cell cycles. In addition, the aggregating/binding process can be easily reversed by adding EDTA, a good chelated ligand of Zn(2+), which gives further proof of the interaction mechanism.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Chengke
C
Wang
Jine
J
Wang
Dianjun
D
Liu
Zhenxin
Z
Wang
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007477:Ions / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D007477:Ions; D010446:Peptide Fragments; D006046:Gold; D015032:Zinc; D002118:Calcium