Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans.
A strain of Bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to L: -lactic acid with 85% yield at 50 degrees C was isolated from composted dairy manure. The strain was tolerant to aldehyde growth inhibitors at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. In a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate of 100 g corn fiber/l to 39 g lactic acid/l in 72 h at 50 degrees C. Because of its inhibitor tolerance and ability to fully utilize pentose sugars, this strain has potential to be developed as a biocatalyst for the conversion of agricultural residues into valuable chemicals.
U.S. Department of Agriculture, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA. kenneth.bischoff@ars.usda.gov
Kenneth M
KM
Bischoff
Siqing
S
Liu
Stephen R
SR
Hughes
Joseph O
JO
Rich
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D008372:Manure / Q000382:microbiology; D003313:Zea mays / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D008372:Manure; D019344:Lactic Acid