Expression and responses to dehydration and salinity stresses of V-PPase gene members in wheat.
Vacuolar H(+)-translocating pyrophosphatase (V-PPase) is a key enzyme related to plant growth as well as abiotic stress tolerance. In this work, wheat V-PPase genes TaVP1, TaVP2 and TaVP3 were identified. TaVP1 and TaVP2 are more similar to each other than to TaVP3. Their deduced polypeptide sequences preserve the topological structure and essential residues of V-PPases. Phylogenetic studies suggested that monocot plants, at least monocot grasses, have three VP paralogs. TaVP3 transcripts were only detected in developing seeds, and no TaVP2 transcripts were found in germinating seeds. TaVP2 was mainly expressed in shoot tissues and down-regulated in leaves under dehydration. Its expression was up-regulated in roots under high salinity. TaVP1 was relatively more ubiquitously and evenly expressed than TaVP2. Its expression level in roots was highest among the tissues examined, and was inducible by salinity stress. These results indicated that the V-PPase gene paralogs in wheat are differentially regulated spatially and in response to dehydration and salinity stresses.
Wang
Yuezhi
Y
Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
Xu
Haibin
H
Zhang
Guangxiang
G
Zhu
Huilan
H
Zhang
Lixia
L
Zhang
Zhengzhi
Z
Zhang
Caiqin
C
Ma
Zhengqiang
Z
D018744:DNA, Plant / Q000187:drug effects; D003681:Dehydration; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D017343:Genes, Plant / Q000187:drug effects* / Q000502:physiology; D054712:Salinity; D012639:Seeds / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000009:adverse effects; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism; D014881:Water Supply
D018744:DNA, Plant; D012965:Sodium Chloride