Phytotherapy of ulcerative dermatitis induced by Aeromonas hydrophila infection in goldfish ( Carassius auratus ).
Goldfish ( Carassius auratus ) weighing 13 +/- 2 g were administered intramuscularly a sublethal dose (1.8 x 10 3 cfu/ml) of Aeromonas hydrophila to induce ulcerative dermatitis. On day 3 and day 6 after infection the fish were dip-treated (for 5 min/day) with a tri-herbal concoction of Curcuma longa, Ocimum sanctum and Azadirachta indica (1%). The LD 50 value was recorded at a concentration of 2.3 x 10 4 between 30 and 36 days after infection. In the infected untreated group the cumulative mortality was higher, while in the early-treated group (day 3) there was no mortality. In the late-treated group (6th day) the mortality increased to 23.3% on day 36. In the infected group the size of ulcers progressively increased from 43.3% of the body length on day 18 to 86.7% on day 36. In the early-treated fish the size of ulcers was 23.3% of the body length on day 18; by day 36 after treatment the ulcer had completely healed. In contrast, there was only a moderate recovery in the late-treated group. From the results it can be concluded that early tri-herbal treatment ensures successful recovery from ulcerative dermatitis induced by A. hydrophila .
Jeju National University Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Science, College of Ocean Science Jeju 690-756 South Korea.
Ramasamy
R
Harikrishnan
Chellam
C
Balasundaram
Young-Gun
YG
Moon
Man-Chul
MC
Kim
Ju-Sang
JS
Kim
Subramanian
S
Dharaneedharan
Moon-Soo
MS
Heo
D016980:Aeromonas hydrophila; D000818:Animals; D031601:Azadirachta / Q000737:chemistry; D030024:Curcuma / Q000737:chemistry; D004334:Drug Administration Schedule / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology* / Q000628:therapy; D006054:Goldfish; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000628:therapy / Q000662:veterinary*; D031339:Ocimum / Q000737:chemistry; D008517:Phytotherapy / Q000662:veterinary*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D017192:Skin Diseases, Bacterial / Q000382:microbiology / Q000628:therapy / Q000662:veterinary*
D010936:Plant Extracts