Altered expression of the small guanosine triphosphatase RhoA in human temporal lobe epilepsy.
The small guanosine triphosphatase RhoA has recently been implicated in the pathogenesis of epilepsy in animals. In this study, we investigated the expression of RhoA in human epilepsy. Brain tissue samples from 40 patients with intractable epilepsy and 14 histologically normal temporal lobes from control patients were used to compare RhoA immunoreactivity using immunohistochemistry, immunofluorescent staining, and western blotting. Immunohistochemical staining and western blot analysis showed that RhoA immunoreactivity was increased in the patient group (p < 0.05). Immunofluorescent staining showed that RhoA was mainly expressed on cell membranes and in the cytoplasm. Our findings demonstrate that upregulation of RhoA immunoreactivity occurs in the brains of patients with intractable epilepsy.
Department of Neurology, The First Affiliated Hospital, Chongqing, Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China.
Jie
J
Yuan
Lin-yuan
LY
Wang
Jin-mei
JM
Li
Ning-jia
NJ
Cao
Liang
L
Wang
Gui-bo
GB
Feng
Tao
T
Xue
Yang
Y
Lu
Xue-feng
XF
Wang
D000293:Adolescent; D000328:Adult; D002648:Child; D004833:Epilepsy, Temporal Lobe / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013702:Temporal Lobe / Q000166:cytology / Q000201:enzymology; D055815:Young Adult; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D020742:rhoA GTP-Binding Protein