GammaH2AX as a molecular marker of aging and disease.
Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form gammaH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete gammaH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of gammaH2AX as a molecular marker of aging and disease is analysed.
Epigenomic Medicine, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, Melbourne, VIC, AU.
Li-Jeen
LJ
Mah
Assam
A
El-Osta
Tom C
TC
Karagiannis
D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004249:DNA Damage; D004194:Disease; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000473:pathology
D015415:Biomarkers; D006657:Histones