Real-time PCR expression profiling of MMPs and TIMPs.
Quantitative reverse transcriptase polymerase chain reaction enables the accurate quantification of gene expression in cultured cells or small tissue samples. In this chapter, we describe the use of Taqman((R)) technology to measure expression of matrix metalloproteinases and related genes.
Pennington
Caroline J
CJ
School of Biological Sciences, University of East Anglia, Norwich, UK.
Edwards
Dylan R
DR
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D015347:RNA Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D048348:Reverse Transcription / Q000235:genetics; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*
D015347:RNA Probes; C034477:RNA primers; D019714:Tissue Inhibitor of Metalloproteinases; D012313:RNA; D020782:Matrix Metalloproteinases