Phytoglycoprotein (75 kDa) suppresses release of histamine and expression of IL-4 and IFN- gamma in BPA-treated RBL-2H3 cells.
The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca(2+) level, and the activities of protein kinase C alpha (PKC alpha), transcription factors NFkappaB and cytokines (IL-4, and IFN-gamma) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca(2+) level, translocation of PKC alpha from cytosol to membrane and the translocation of NF-kappaB in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-gamma) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-gamma via down regulations of Ca(2+)/PKCalpha and NF-kappaB on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA.
Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, Gwang-ju, 300, Yongbong-Dong, South Korea.
Cheol-Ho
CH
Park
Kye-Taek
KT
Lim
D000395:Air Pollutants, Occupational / Q000633:toxicity; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001559:Benzhydryl Compounds; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D004336:Drug Antagonism; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D029586:Moraceae / Q000737:chemistry*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D010636:Phenols / Q000633:toxicity; D010940:Plant Proteins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098826:Bisphenol A Compounds
D000395:Air Pollutants, Occupational; D018926:Anti-Allergic Agents; D001559:Benzhydryl Compounds; D002118:Calcium; D006023:Glycoproteins; D007371:Interferon-gamma; D015847:Interleukin-4; D016328:NF-kappa B; D010636:Phenols; D010940:Plant Proteins; D011493:Protein Kinase C; C006780:bisphenol A; D000098826:Bisphenol A Compounds