Extraction of DNA from the plant Kalanchoë daigremontiana.
This protocol describes how to isolate genomic DNA from leaves and stems of Kalanchoë daigremontiana. The procedure can be applied to adult leaves, but it is best to use younger leaves because they have fewer secondary metabolites and polysaccharides, which can interfere with the DNA extraction. The resulting DNA can be used for polymerase chain reactions (PCRs), Southern blots, or other applications.
Garcês
Helena
H
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Sinha
Neelima
N
D001901:Botany / Q000379:methods; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005821:Genetic Techniques; D031962:Kalanchoe / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism
D018744:DNA, Plant