Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P.
3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO(3) to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30 degrees C.
Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan. toyosida@gifu-u.ac.jp
Toyokazu
T
Yoshida
Yuki
Y
Inami
Tsuyoshi
T
Matsui
Toru
T
Nagasawa
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001639:Bicarbonates / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D003067:Coenzymes / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D016972:Enterobacter cloacae / Q000201:enzymology* / Q000378:metabolism*; D005707:Gallic Acid / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology; D013237:Stereoisomerism; D062366:para-Aminobenzoates
D001426:Bacterial Proteins; D001639:Bicarbonates; D002396:Catechols; D003067:Coenzymes; D003470:Culture Media; D062385:Hydroxybenzoates; D017680:Potassium Compounds; D062366:para-Aminobenzoates; C023730:Acedoben; C032948:3-hydroxybenzoic acid; C009091:protocatechuic acid; D005707:Gallic Acid; D002262:Carboxy-Lyases; C099799:3,4-dihydroxybenzoate decarboxylase; C026329:potassium bicarbonate; C034221:catechol; D010129:4-Aminobenzoic Acid