Isolation and characterization of a novel glycogen synthase kinase-3 gene, GmGSK, in Glycine max L. that enhances abiotic stress tolerance in Saccharomyces cerevisiae.
A novel glycogen synthase kinase-3 gene, GmGSK, was isolated from Glycine max. It is 1,596 bp in length with one ORF of 410 amino acids. Southern blot analysis revealed that it has at least two copies in the G. max genome. GmGSK, when transiently expressed in Nicotiana tabacum leaves, was localized in both cell membrane and cytoplasm. Northern blot analysis indicated that GmGSK is expressed in all tissues, with highest expression in the root. GmGSK can be induced by various abiotic stresses. When transformed with GmGSK, Saccharomyces cerevisiae exhibited enhanced resistance to salt and drought stress.
College of Life Science, Northeast Normal University, 130024, Changchun, People's Republic of China.
Chunbao
C
Zhang
Hongkun
H
Zhao
Yanzhi
Y
Liu
Qiyun
Q
Li
Xiaodong
X
Liu
Hua
H
Tan
Cuiping
C
Yuan
Yingshan
Y
Dong
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002462:Cell Membrane / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D020869:Gene Expression Profiling; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000201:enzymology*; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics; D019076:Transgenes
D018744:DNA, Plant; D011994:Recombinant Proteins; D038362:Glycogen Synthase Kinase 3