Small-molecule screen identifies reactive oxygen species as key regulators of neutrophil chemotaxis.
Neutrophil chemotaxis plays an essential role in innate immunity, but the underlying cellular mechanism is still not fully characterized. Here, using a small-molecule functional screening, we identified NADPH oxidase-dependent reactive oxygen species as key regulators of neutrophil chemotactic migration. Neutrophils with pharmacologically inhibited oxidase, or isolated from chronic granulomatous disease (CGD) patients and mice, formed more frequent multiple pseudopodia and lost their directionality as they migrated up a chemoattractant concentration gradient. Knocking down NADPH oxidase in differentiated neutrophil-like HL60 cells also led to defective chemotaxis. Consistent with the in vitro results, adoptively transferred CGD murine neutrophils showed impaired in vivo recruitment to sites of inflammation. Together, these results present a physiological role for reactive oxygen species in regulating neutrophil functions and shed light on the pathogenesis of CGD.
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Hidenori
H
Hattori
Kulandayan K
KK
Subramanian
Jiro
J
Sakai
Yonghui
Y
Jia
Yitang
Y
Li
Timothy F
TF
Porter
Fabien
F
Loison
Bara
B
Sarraj
Anongnard
A
Kasorn
Hakryul
H
Jo
Catlyn
C
Blanchard
Dorothy
D
Zirkle
Douglas
D
McDonald
Sung-Yun
SY
Pai
Charles N
CN
Serhan
Hongbo R
HR
Luo
D000818:Animals; D002633:Chemotaxis; D004353:Drug Evaluation, Preclinical; D055785:Gene Knockdown Techniques; D006105:Granulomatous Disease, Chronic / Q000201:enzymology / Q000276:immunology*; D018922:HL-60 Cells; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054852:Small Molecule Libraries
D017382:Reactive Oxygen Species; D054852:Small Molecule Libraries; D019255:NADPH Oxidases