Immunodiagnosis of Chagas disease: Synthesis of three latex-protein complexes containing different antigens of Trypanosoma cruzi.
This article describes the physical adsorption and the chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes to be used as novel immunodiagnosis reagents for detecting the Chagas disease. The coupled proteins were a homogenate of T. cruzi, or a recombinant protein (either Ag36 or CP1). With the homogenate, between 30 and 60% of the total-linked protein was chemically coupled, showing a small dependence with the pH. For Ag36 and CP1, around 90% of the total-linked protein was chemically coupled, with a maximum coupling at pH 5 (i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36.
INTEC, Universidad Nacional del Litoral and CONICET, Güemes, Santa Fe, Argentina.
Verónica D G
VD
Gonzalez
Valeria S
VS
Garcia
Jorge R
JR
Vega
Ivan S
IS
Marcipar
Gregorio R
GR
Meira
Luis M
LM
Gugliotta
D000953:Antigens, Protozoan / Q000032:analysis*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007840:Latex / Q000737:chemistry*; D011994:Recombinant Proteins / Q000138:chemical synthesis; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D007840:Latex; D011994:Recombinant Proteins