Simultaneous quantification of 14 bioactive constituents in Forsythia suspensa by liquid chromatography-electrospray ionisation-mass spectrometry.
BACKGROUND
Forsythia suspensa is a commonly used traditional Chinese medicine including phenylethanoid glycosides, lignans, flavonoids, terpenes and volatile oils. Quantification of multi-components is important for the quality control of Forsythia suspensa.


OBJECTIVE
To establish a liquid chromatography-electrospray ionisation-mass spectrometry method for simultaneous quantification of 14 bioactive constituents of Forsythia suspensa in different places of China and different parts of this herb.


METHODS
The optimal chromatographic conditions were achieved on a Kromasil C(18) column (150 yen 4.6 mm, 5 microm) with gradient elution of methanol, acetonitrile and 0.1% formic acid in 27 min. Detection was performed in negative ionisation mode by monitoring the precursor-product combination in multiple reaction monitoring (MRM) mode. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy.


RESULTS
All calibration curves showed good linear regression (r > 0.9990) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.7-4.3 and 1.1-3.9% respectively, and overall recoveries of 96.65-101.2% for the compounds analysed.


CONCLUSIONS
The proposed method was successfully applied for the quantitative analysis of 14 constituents in 12 Forsythia suspensa samples.
Cui
Yang
Y
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, People's Republic of China.
Wang
Qiao
Q
Shi
Xiaowei
X
Zhang
Xiaowei
X
Sheng
Xiaona
X
Zhang
Lantong
L
D002109:Caffeic Acids / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D031657:Forsythia / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D006569:Hesperidin / Q000032:analysis; D017705:Lignans / Q000032:analysis; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D017705:Lignans; D002726:Chlorogenic Acid; D012431:Rutin; C037876:suspensaside; C542294:phillygenin; C021304:hyperoside; D011794:Quercetin; D006569:Hesperidin; C058049:forsythiaside; C075528:phillyrin