Lactobacillus-mediated RNA interference in nematode.
We engineered Lactobacillus paracasei to produce a dsRNA that would trigger RNAi-induced silencing of an essential gene in the nematode Caenorhabditis elegans. The dsRNA-expressing L. paracasei can be used in experiments conducted on culture plates and may also be used as an orally administrable dsRNA carrier for humans and other mammals.
Kuwahara
Ai
A
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama, Japan.
Arita
Masashi
M
Kushiro
Akira
A
Sakube
Yasuji
Y
Sisido
Masahiko
M
Ohtsuki
Takashi
T
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D006801:Humans; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism; D009348:Nematoda / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction