Expression of recombinant MMP-28 in mammalian cells.
The expression of a recombinant MMP in a mammalian cell line can be useful, e.g., for purification of the enzyme, to characterize function of the enzyme, or to uncover its substrates. In this chapter, we have therefore documented our experience with the recently discovered MMP-28.
Rodgers
Ursula R
UR
School of Biological Sciences, University of East Anglia, Norwich, UK.
Clark
Ian M
IM
D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation; D006801:Humans; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D050505:Mutant Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*; D014162:Transfection
D018076:DNA, Complementary; D050505:Mutant Proteins; D011994:Recombinant Proteins; D053505:Matrix Metalloproteinases, Secreted