Photobiological activity of Ru(II) dyads based on (pyren-1-yl)ethynyl derivatives of 1,10-phenanthroline.
Several mononuclear Ru(II) dyads possessing 1,10-phenanthroline-appended pyrenylethynylene ligands were synthesized, characterized, and evaluated for their potential in photobiological applications such as photodynamic therapy (PDT). These complexes interact with DNA via intercalation and photocleave DNA in vitro at submicromolar concentrations when irradiated with visible light (lambda(irr) > or = 400 nm). Such properties are remarkably sensitive to the position of the ethynylpyrenyl substituent on the 1,10-phenanthroline ring, with 3-substitution showing the strongest binding under all conditions and causing the most deleterious DNA damage. Both dyads photocleave DNA under hypoxic conditions, and this photoactivity translates well to cytotoxicity and photocytotoxicity models using human leukemia cells, where the 5- and 3-substituted dyads show photocytotoxicity at 5-10 microM and 10-20 microM, respectively, with minimal, or essentially no, dark toxicity at these concentrations. This lack of dark cytotoxicity at concentrations where significant photoactivity is observed emphasizes that agents with strong intercalating units, previously thought to be too toxic for phototherapeutic applications, should not be excluded from the arsenal of potential photochemotherapeutic agents under investigation.
Department of Chemistry, Acadia University, Wolfville, NS B4P 2R6, Canada.
Susan
S
Monro
John
J
Scott
Abdellatif
A
Chouai
Richard
R
Lincoln
Ruifa
R
Zong
Randolph P
RP
Thummel
Sherri A
SA
McFarland
D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010618:Phenanthrolines / Q000737:chemistry / Q000494:pharmacology*; D018462:Photobiology; D017975:Ruthenium Compounds / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D010618:Phenanthrolines; D017975:Ruthenium Compounds; D004247:DNA; C025205:1,10-phenanthroline