Probiotic Lactobacillus strains: in vitro and in vivo studies.
Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine T(H)1-T(H)2 balance toward nonallergic T(H)1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-gamma than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.
Cukrowska
B
B
Department of Pathology, The Children's Memorial Health Institute, Warsaw, Poland. b.cukrowska@czd.pl
Motyl
I
I
Kozáková
H
H
Schwarzer
M
M
Górecki
R K
RK
Klewicka
E
E
Slizewska
K
K
Libudzisz
Z
Z
D000143:Acids / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D018988:Bacterial Translocation; D001647:Bile Acids and Salts / Q000494:pharmacology; D001769:Blood / Q000382:microbiology; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D007778:Lactobacillus / Q000187:drug effects / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D013312:Stress, Physiological; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000143:Acids; D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma