[The etiology and clinical manifestations of 70 patients with hand-foot-mouth disease].
OBJECTIVE
To analyze the etiology and clinical manifestations of 70 patients with hand-foot-mouth disease (HFMD).


METHODS
The viral RNA from the pharynx swab samples were extracted and collected from 70 HFMD patients (of which, 60 cases were under 5 years old) to detect the 5'-UTR gene of EV, the Vp3-Vp1 genes of EV71 and Cox-A16 by utilizing the technique of RT-PCR.


RESULTS
Of 70 HFMD patients, 30 cases (42.8%, 30/70) were positive in enterovirus nucleic acid, including EV71 and Cox-A16, pharynx swab sample testing. While, of the enteroviral pathogen RNA-positive 30 cases, EV71 accounted for 66.7% (20/30). The proportion of etiological positive of 39 samples collected within 4 days after onset was 66.7% (26/39), but the probability of 31 samples collected after more than 5 days after onset was 12.9% (4/31), the difference was significantly (chi(2) = 20.4, P < 0.01).


CONCLUSIONS
HFMD patients were mainly infants and young children between 0-5 years old, but adults could also be attacked. The enterovirus detected was mainly EV71, and the pharynx swab samples should be collected within 4 days after onset to increase the related viral nucleic acid positive detection probability.
Zhang
Xin
X
Research Center for Infection and Immunity of Beijing Youan Hospital, Capital Medical University, China.
Yan
Hui-ping
HP
Huang
Chun
C
Tan
Yu-fen
YF
Ma
Dong-mei
DM
Zhang
Hai-ping
HP
Liu
Yan
Y
Wang
Shu-zhao
SZ
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000235:genetics*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000209:etiology* / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D055815:Young Adult
D012367:RNA, Viral