Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells.
Silk fibroin, produced by the silkworm Bombyx mori, has been widely studied as a scaffold in tissue engineering. Although it has been shown to be slowly biodegradable, cellular responses to degraded silk fibroin fragments are largely unknown. In this study, silk fibroin was added to MG-63 cell cultures, and changes in gene expression in the MG-63 cells were screened by DNA microarray analysis. Genes showing a significant (2-fold) change were selected and their expression changes confirmed by quantitative RT-PCR and western blotting. DNA microarray results showed that alkaline phosphatase (ALP), collagen type-I alpha-1, fibronectin, and transforming growth factor-beta1 expressions significantly increased. The effect of degraded silk fibroin on osteoblastogenic gene expression was confirmed by observing up-regulation of ALP activity in MG-63 cells. The finding that small fragments of silk fibroin are able to increase the expression of osteoblastogenic genes suggests that controlled degradation of silk fibroin might accelerate new bone formation. [BMB reports 2010; 43(1): 52-56].
Kim
Jwa-Young
JY
Department of Oral and Maxillofacial Surgery, Hallym University, Chuncheon 200-702, Korea.
Choi
Je-Yong
JY
Jeong
Jae-Hwan
JH
Jang
Eun-Sik
ES
Kim
An-Sook
AS
Kim
Seong-Gon
SG
Kweon
Hae Yong
HY
Jo
You-Young
YY
Yeo
Joo-Hong
JH
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D012831:Bombyx / Q000378:metabolism; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D000090602:Collagen Type I, alpha 1 Chain; D005349:Fibroins / Q000494:pharmacology*; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D046228:Microarray Analysis; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
C000716127:COL1A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D005353:Fibronectins; D053773:Transforming Growth Factor beta1; D005349:Fibroins; D000469:Alkaline Phosphatase