Quantitative analysis of gamma-hydroxybutyrate at endogenous concentrations in hair using liquid chromatography tandem mass spectrometry.
A method capable of quantifying endogenous concentrations of gamma-hydroxybutyrate (GHB) in human head hair was developed and validated using liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). Hair was digested under alkaline conditions, and GHB was isolated using liquid-liquid extraction. LC/MS/MS was performed using atmospheric pressure chemical ionization in the negative mode, multiple reaction monitoring, and deuterated internal standard (GHB-D(6)). Linearity was observed between 0.1 and 100 ng/mg GHB (R(2) = 1.000). The limits of detection and quantitation in human hair were 0.2 and 0.4 ng/mg, respectively. Accuracy at 2 ng/mg and 10 ng/mg was determined to be 97% and 94%, and intra-assay CVs at these concentrations were 5.2% and 7.4% (n = 4). Beta-hydroxybutyrate (BHB), alpha-hydroxybutyrate, gamma-butyrolactone, and 1,4-butanediol did not produce an interference, and there was negligible ion suppression or enhancement from the matrix.
Stout
Phillip A
PA
Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341, USA.
Simons
Kelsie D
KD
Kerrigan
Sarah
S
D020155:3-Hydroxybutyric Acid / Q000494:pharmacology; D015107:4-Butyrolactone / Q000494:pharmacology; D002072:Butylene Glycols / Q000494:pharmacology; D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D006197:Hair / Q000378:metabolism*; D006801:Humans; D012978:Sodium Oxybate / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D002072:Butylene Glycols; D012978:Sodium Oxybate; C039681:1,4-butanediol; D015107:4-Butyrolactone; D020155:3-Hydroxybutyric Acid