Analysis of the degradome with the CLIP-CHIP microarray.
The degradome microarray - CLIP-CHIP - is a dedicated and focused array that allows the analysis of all proteases, non-proteolytic homologs, and protease inhibitor gene transcripts in the human and murine genomes at the mRNA transcript level.Based on unique 70-mer oligonucleotides, designed to match parts of the sequence of known or predicted protease and inhibitor mRNAs in both species and printed on a glass-matrix surface, the CLIP-CHIP microarray can be used to analyze differentially expressed protease and inhibitor gene products and give expression profiles for any analyzed sample.
Kappelhoff
Reinhild
R
Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
Auf dem Keller
Ulrich
U
Overall
Christopher M
CM
D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification; D013223:Statistics as Topic
D005456:Fluorescent Dyes; D020543:Proteome; D012313:RNA