Microemulsion-based organogels as matrices for lipase immobilization.
Organogels based on water-in-oil microemulsions can be formed using various natural polymers such as gelatin, agar or cellulose derivatives. Enzymes entrapped in the water core of the microemulsion can keep their activity and enhance their stability within the gel matrix. The importance of the microemulsion based organogels (MBGs) leans on their numerous potential biotechnological applications. An important example is the use of various lipase microemulsion systems for hydrolytic or synthetic reactions. In this review, several MBGs are being evaluated as immobilization matrices for various enzymes. The main subject focuses on the parameters that affect the use of MBGs as media for bioorganic reactions using lipases as catalysts.
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave 11635, Athens, Greece.
Maria
M
Zoumpanioti
Haralambos
H
Stamatis
Aristotelis
A
Xenakis
D000327:Adsorption; D004655:Emulsions / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D011485:Protein Binding
D004655:Emulsions; D004800:Enzymes, Immobilized; D005782:Gels; D009930:Organic Chemicals; D008049:Lipase