Effect of 3'-end capping of aptamer with various 2',4'-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers.
The capping of the 3'-ends of thrombin binding aptamers (TBAs) with bridged nucleotides increased the nuclease resistances and the stabilities in human serum. The binding abilities of the aptamers were not affected by the capping. The capping could be simply executed via a one step enzymatic process using 2',4'-bridged nucleoside 5'-triphosphate and terminal deoxynucleotidyl transferase.
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan.
Yuuya
Y
Kasahara
Shunsuke
S
Kitadume
Kunihiko
K
Morihiro
Masayasu
M
Kuwahara
Hiroaki
H
Ozaki
Hiroaki
H
Sawai
Takeshi
T
Imanishi
Satoshi
S
Obika
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D006801:Humans; D009711:Nucleotides / Q000737:chemistry*; D043264:Phosphodiesterase I / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D013997:Time Factors
D052157:Aptamers, Nucleotide; D009711:Nucleotides; C081676:thrombin aptamer; D004253:DNA Nucleotidylexotransferase; D043264:Phosphodiesterase I