Study of potential cytoprotective activity of different antioxidants.
It is known that infiltration of atherosclerotic plaques by activated T lymphocytes occurs at the early stage and cytokines produced by those lymphocytes become actively involved in inflammatory process. Consequently the preparations, capable of stabilization of immune cells, perhaps will be effective for treatment and prevention of atherosclerosis. Intensively proliferated Jurkat leukemic T cell line (human leukemic cell culture) is widely used in scientific and Therapeutic research, as human T lymphocyte pattern. The aim of the study--To establish the cytoprotective action of different antioxidative preparations on Jurkat cells incubated in oxidative stress conditions. It was revealed the dose (100 microl, 50 microl) and time-related decrease of viability of Jurkat cells incubated in high oxidative stress conditions. Low doses of hydrogen peroxide (H(2)O(2)) (25 microl, 11 microl) present cytotoxicity only within short terms (8 hours). Vitamins C and E have cytotoxic effects on intact Jurkat cells separately; the activity of Megavite and Phenovin is not characterised by cytotoxicity. Incubation of Jurkat cells with antioxidants in moderate oxidative stress condition (H(2)O(2) 25 microl, 50 microl) all antioxidative preparations (except vitamin C) reveal static activity on viability of Jurkat cells. Incubation of Jurkat cells with antioxidants in intensive oxidative stress condition (H(2)O(2) 100 microl), the cytoprotective activity of antioxidative preparations was rather low.
Tbilisi State Medical University.
L
L
Ratiani
T
T
Ratiani
G
G
Sharashenidze
G
G
Janigashvili
T
T
Sanikidze
D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D050197:Atherosclerosis / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D015551:Autoimmunity / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D006801:Humans; D019169:Jurkat Cells; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D010636:Phenols