Early transcriptional response to chloroquine of the Plasmodium falciparum antioxidant defence in sensitive and resistant clones.
Resistance to chloroquine (CQ) in Plasmodium falciparum has a major impact on malaria control worldwide. To gain insight into early parasite stress response, mRNA expression profiles were determined for a set of 10 antioxidant defence genes in synchronized CQ-sensitive (3D7) and CQ-resistant (Dd2) clones under transient IC50 CQ-exposure (Dd2, 200 nM; 3D7, 14 nM). Upon 2-h CQ challenge, the mRNA upregulation detected was greater in 3D7 (six genes overexpressed at 1/3 of the intraerythrocytic cycle) than in Dd2 clone (three genes responding), providing evidence of an early transcriptional response to CQ-induced oxidative stress which might underlie some of the parasite's metabolic adaptation to the drug.
Nogueira
Fátima
F
Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain.
Diez
Amalia
A
Radfar
Azar
A
Pérez-Benavente
Susana
S
do Rosario
Virgilio E
VE
Puyet
Antonio
A
Bautista
José M
JM
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D004351:Drug Resistance; D020869:Gene Expression Profiling; D020128:Inhibitory Concentration 50; D008954:Models, Biological; D018384:Oxidative Stress; D010963:Plasmodium falciparum / Q000187:drug effects*; D013312:Stress, Physiological; D013997:Time Factors
D000962:Antimalarials; D002738:Chloroquine