Taqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine.
Western flower thrip (Frankliniella occidentalis) is a major global pest of agricultural products. It directly damages crops through feeding, oviposition activity or transmission of several plant viruses. We describe a Taqman real-time quantitative PCR detection system, which can rapidly identify F. occidentalis from thrips larvae to complement the traditional morphological identification. The data showed that our detection system targeted on the ribosomal RNA gene regions of F. occidentalis has high sensitivity and specificity. The rapid method can be used for on-site testing of samples at ports-of-entry in the future.
Huang
K S
KS
Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Hsinchu Branch, Tao-Yuan, Taiwan, Republic of China.
Lee
S E
SE
Yeh
Y
Y
Shen
G S
GS
Mei
E
E
Chang
C M
CM
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000378:metabolism; D007313:Insecta / Q000235:genetics*; D010935:Plant Diseases / Q000469:parasitology* / Q000517:prevention & control*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011790:Quarantine / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism*
D017931:DNA Primers; D015342:DNA Probes; D012335:RNA, Ribosomal; D019914:Taq Polymerase