[Effect of Cordyceps Cinensis extractant on apoptosis and expression of Toll-like receptor 4 mRNA in the ischemia-reperfusion injured NRK-52E cells].
OBJECTIVE
To observe the apoptosis and expression of Toll-like receptor (TLR)-4's mRNA in ischemia-reperfusion injured rat renal tubular epithelia cells (NRK-52E) in vitro, and effect of Cordyceps Sinensis (CS) extractant.


METHODS
Cultured NRK-52E cells were divided into a control group, a model group, and a CS preincubated group. All first removed media and incubated with antimycin A for 1 h, and then recovered the media to simulate the ischemia-reperfusion injury in vitro. We detected the apoptosis ratio of cells by flow cytometer and the mRNA expression of TLR-4, Bax, Bcl-2 gene by reverse transcription-polymerase chain reaction at different time points.


RESULTS
In ischemia-reperfusion injured NRK-52E, the apoptosis ratio rose as time passed (P<0.05). We also observed increased mRNA expression of TLR-4, Bax (P<0.05) and deceased expression of Bcl-2 (P<0.05). Compared with the model group, the CS preincubated NRK-52E cells showed apparent tolerance to ischemia-reperfusion injury, which manifestated lower apoptosis ratio (P<0.05), decreased expression of mRNA of TLR-4, Bax and increased expression of Bcl-2 (All Ps<0.05).


CONCLUSIONS
The number of apoptosis cells and the expression of TLR-4 mRNA increased with ischemia-reperfusion injury of NRK-52E in vitro. CS can prevent the NRK-52E cells from ischemia-reperfusion injury by downregulating TLR-4 gene.
Zhou
Qiaoling
Q
Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China. zhouqling@yahoo.com.cn
Hu
Sai
S
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015687:Cell Hypoxia; D002460:Cell Line; D032661:Cordyceps / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology; D007684:Kidney Tubules / Q000166:cytology; D008419:Materia Medica / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
D008419:Materia Medica; D012333:RNA, Messenger; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4