Comparison of lipid profiles in relation to APOB EcoRI polymorphism in obese children with hyperlipidemia.
BACKGROUND
We aimed to evaluate apolipoprotein B-100 (APOB) EcoRI polymorphism and plasma lipid parameters together in children and adolescents. This is the first such study in Turkey to determine possible relationships of these parameters.


METHODS
Three separate groups were studied: a group of obese children with hyperlipidemia, a group of obese children without hyperlipidemia, and a group of healthy children neither with hyperlipidemia nor obesity. Polymerase chain reaction (PCR), denaturating gradient gel electrophoresis (DGGE) and automatic sequence analysis techniques were used. Sequencing results were examined by Proseq and BioEdit computer programmes.


RESULTS
Mutant A allele was not observed in the healthy control group, whereas it was more frequent in the hyperlipidemic obese children; the GA genotype was correlated with total and low density lipoprotein-cholesterol levels.


CONCLUSIONS
In this study, we suggest that obese child patients having the A allele could have a higher risk for developing hyperlipidemia.
Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul University, Capa-Istanbul, Turkey.
Ozlem
O
Timirci
Feyza
F
Darendeliler
Firdevs
F
Bas
Ergen H
EH
Arzu
Zeybek
Z
Umit
Turgay
T
Isbir
D000293:Adolescent; D001055:Apolipoproteins B / Q000235:genetics*; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D009765:Obesity / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology
D001055:Apolipoproteins B; C020098:DNA modification methylase EcoRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)