Relationship between gene expressions of aquaporin 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis.
OBJECTIVE
To explore the relationship between gene expressions of aquaporin (AQP) 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis (CSG).


METHODS
Twenty-four CSG patients were divided into three groups according to the degrees of spleen-stomach dampness-heat syndrome: mild, moderate and severe groups. There were 8 patients in each group, and another 8 healthy persons were selected as normal control. Samples of mucosa of upper stomach in the included patients and normal persons were collected by gastroscopy, and then were stored in liquid nitrogen. The gene expressions of AQP3 and AQP4 in gastric tissue were examined by fluorescence quantitative polymerase chain reaction.


RESULTS
The gene expressions of AQP3 and AQP4 in the moderate and severe groups were higher than that in the mild group and normal group (P<0.05, P<0.01). The gene expression of AQP3 in the severe group was higher than that in the moderate group (P<0.05).


CONCLUSIONS
The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of CSG. There is a relationship between expressions of AQP3 and AQP4 genes and the degrees of spleen-stomach dampness-heat syndrome, and the gene expressions will increase with the aggravation of the dampness-heat syndrome.
Mei
Wu-xuan
WX
Department of Internal Medicine, Xianning Medical College, Xianning 437100, Hubei Province, China. meifeil@163.com
Lao
Shao-xian
SX
Yu
Na
N
Zhou
Zheng
Z
Huang
Lie-ping
LP
Hu
Bin
B
D000328:Adult; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000175:diagnosis / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged
C496027:AQP3 protein, human; C496030:AQP4 protein, human; D051401:Aquaporin 4; D051400:Aquaporin 3