In vitro skin permeation of hinokitiol loaded in vesicles composed of behenyltrimethylammonium chloride and stearic acid.
BACKGROUND
Behenyltrimethylammonium chloride (BTAC) and stearic acid (SA) could be associated with each other through salt bridges, and the associated BTAC/SA could build bilayer vesicles with the aid of hinokitiol (HKL).


METHODS
The vesicles were prepared by a precipitation method and used to enhance the skin permeation of HKL.


RESULTS
In case the molar ratio of BTAC/SA/HKL was 1/1/0, no vesicle was observed on transmission electron microscope photos. When the molar ratio of BTAC/SA/HKL was 1/1/0.4, vesicle was observed together with some agglomerates. When the content of HKL increased to the ratios of 1/1/0.8 and 1/1/1.2, vesicles were exclusively observed. In vitro fluxes for 18 hours through hairless mouse skin of HKL dissolved in alcoholic solutions were less than 1 mg/cm2/h. Whereas the fluxes of HKL encapsulated in the vesicles were about three times higher than that of HKL in the alcoholic solutions.


CONCLUSIONS
The vesicles could be used for the hair growth promotion.
Yang
Xia
X
Department of Biotechnology and Bioengineering, School of Biotechnology and Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, Chunchon, Korea.
Lee
Hyeon Yong
HY
Kim
Jin-Chul
JC
D000279:Administration, Cutaneous; D000818:Animals; D002712:Chlorides / Q000008:administration & dosage / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage / Q000493:pharmacokinetics; D005260:Female; D008744:Methylamines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051379:Mice; D008812:Mice, Hairless; D039821:Monoterpenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010539:Permeability / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology*; D013229:Stearic Acids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014334:Tropolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D002712:Chlorides; D004337:Drug Carriers; D008744:Methylamines; D039821:Monoterpenes; D013229:Stearic Acids; C031183:stearic acid; D014334:Tropolone; C027451:methylamine; C009479:beta-thujaplicin