Early transcriptional response to chloroquine of the Plasmodium falciparum antioxidant defence in sensitive and resistant clones.
Resistance to chloroquine (CQ) in Plasmodium falciparum has a major impact on malaria control worldwide. To gain insight into early parasite stress response, mRNA expression profiles were determined for a set of 10 antioxidant defence genes in synchronized CQ-sensitive (3D7) and CQ-resistant (Dd2) clones under transient IC50 CQ-exposure (Dd2, 200 nM; 3D7, 14 nM). Upon 2-h CQ challenge, the mRNA upregulation detected was greater in 3D7 (six genes overexpressed at 1/3 of the intraerythrocytic cycle) than in Dd2 clone (three genes responding), providing evidence of an early transcriptional response to CQ-induced oxidative stress which might underlie some of the parasite's metabolic adaptation to the drug.
Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain.
Fátima
F
Nogueira
Amalia
A
Diez
Azar
A
Radfar
Susana
S
Pérez-Benavente
Virgilio E
VE
do Rosario
Antonio
A
Puyet
José M
JM
Bautista
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D004351:Drug Resistance; D020869:Gene Expression Profiling; D020128:Inhibitory Concentration 50; D008954:Models, Biological; D018384:Oxidative Stress; D010963:Plasmodium falciparum / Q000187:drug effects*; D013312:Stress, Physiological; D013997:Time Factors
D000962:Antimalarials; D002738:Chloroquine