Respiratory viral RNA on toys in pediatric office waiting rooms.
BACKGROUND
Toys in pediatric office waiting rooms may be fomites for transmission of viruses.


METHODS
Eighteen samples were taken from office objects on 3 occasions. Samples were tested for presence of picornavirus (either rhinovirus or enterovirus) on all 3 sample days; in addition, January samples were tested for respiratory syncytial virus and March samples were tested for influenza A and B. In addition, 15 samples were obtained from the sick waiting room before and after cleaning. Polymerase chain reaction was used to detect picornavirus, respiratory syncytial virus, and influenza A or B virus. Finally, 20 samples were obtained from the fingers of a researcher after handling different toys in the sick waiting room, and samples were then obtained from all the same toys; all samples were tested for picornavirus by polymerase chain reaction.


RESULTS
Viral RNA was detected on 11 of 52 (21%) of toys sampled. Ten of the positives were picornavirus; 1 was influenza B virus. Three (30%) of 10 toys from the new toy bag, 6 of 30 (20%) in the sick child waiting room, and 2 of 12 (17%) in the well child waiting room were positive. Six (40%) of 15 toys in the sick waiting room were positive for picornaviral RNA before cleaning; after cleaning, 4 (27%) of 15 were positive in spite of the fact that RNA was removed from 4 of 6 of the original positives. Three (15%) of 20 toys in the sick waiting room were positive for picornaviral RNA, but RNA was not transferred to the fingers of the investigator who handled these toys.


CONCLUSIONS
About 20% of the objects in a pediatric office may be contaminated with respiratory viral RNA, most commonly picornavirus RNA. Cleaning with a disinfectant cloth was only modestly effective in removing the viral RNA from the surfaces of toys, but transfer of picornaviral RNA from toys to fingers was inefficient.
Department of Pediatrics, University of Virginia Health System, Charlottesville, VA 22908, USA. dep6b@virginia.edu
Diane E
DE
Pappas
J Owen
JO
Hendley
Richard H
RH
Schwartz
D002648:Child; D002675:Child, Preschool; D004783:Environmental Microbiology; D005260:Female; D006225:Hand / Q000821:virology; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010824:Physicians' Offices; D010849:Picornaviridae / Q000235:genetics / Q000302:isolation & purification*; D010988:Play and Playthings; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral