Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 degrees C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.
Laboratory of Plant Breeding, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Japan.
Takuji
T
Tsukiyama
Jongwon
J
Lee
Yutaka
Y
Okumoto
Masayoshi
M
Teraishi
Takatoshi
T
Tanisaka
Kuniyo
K
Inouye
D003001:Cloning, Molecular; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D010940:Plant Proteins; D025801:Ubiquitin