Isolation of individual cells and tissues from electroporated sea squirt (Ciona) embryos by fluorescence-activated cell sorting (FACS).
Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA. lc121@nyu.edu
Lionel
L
Christiaen
Eileen
E
Wagner
Weiyang
W
Shi
Michael
M
Levine
D000818:Animals; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D002938:Ciona intestinalis / Q000196:embryology* / Q000235:genetics; D018274:Electroporation; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D046228:Microarray Analysis; D023421:Models, Animal; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012313:RNA