Comparative cytogenetic analysis of Avena macrostachya and diploid C-genome Avena species.
The chromosome set of Avena macrostachya Balansa ex Coss. et Durieu was analyzed using C-banding and fluorescence in situ hybridization with 5S and 18S-5.8S-26S rRNA gene probes, and the results were compared with the C-genome diploid Avena L. species. The location of major nucleolar organizer regions and 5S rDNA sites on different chromosomes confirmed the affiliation of A. macrostachya with the C-genome group. However, the symmetric karyotype, the absence of "diffuse heterochromatin" and the location of large C-band complexes in proximal chromosome regions pointed to an isolated position of A. macrostachya from other Avena species. Based on the distribution of rDNA loci on the C-genome chromosomes of diploid and polyploid Avena species, we propose a model of the chromosome alterations that occurred during the evolution of oat species.
Badaeva
Ekaterina D
ED
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, Moscow, Russia. K_Badaeva@mail.ru
Shelukhina
Olga Yu
OY
Diederichsen
Axel
A
Loskutov
Igor G
IG
Pukhalskiy
Vitaly A
VA
D018554:Avena / Q000235:genetics*; D002871:Chromosome Banding; D032461:Chromosomes, Plant; D018744:DNA, Plant; D004275:DNA, Ribosomal / Q000235:genetics; D004171:Diploidy; D019143:Evolution, Molecular; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D013045:Species Specificity
D018744:DNA, Plant; D004275:DNA, Ribosomal