Effect of pituitary growth hormone and insulin-like growth factor type-I on proliferation, apoptosis and hormone secretion of the placental cell line JEG-3.
OBJECTIVE
Placenta human choriocarcinoma JEG-3 cells were used to study the possibility that pituitary growth hormone (GH) and insulin-like growth factor type I (IGF-I) act on first trimester of pregnancy progesterone (P4) and human chorionic gonadotropin (hCG) secretion, cell proliferation and apoptosis.


METHODS
The JEG-3 cell line was cultured in Dulbecco modified eagle medium without phenol red containing 10% FBS. The cells were plated in 96-well plates at the density of 3 x 10(3) for 24 h and treated with 10, 50, 100, or 200 ng/ml of GH or 10, 30, 100, or 250 ng/ml of IGF-I for 24 h. At the end of the culture period, cell proliferation was measured using the BrdU colorimetric assay and caspase-3 activity, as a marker of apoptosis, was determined by the colorimetric method. Media were frozen for hormone analysis by enzyme immunoassay. Results. We found that the stimulatory activities of GH and IGF-I on both P4 and hCG secretion paralleled the stimulation of cell proliferation and inhibition of apoptosis.


CONCLUSIONS
Our findings suggest an important role for pituitary GH and IGF-I in normal placental function during the first trimester of pregnancy.
Rak-Mardyła
Agnieszka
A
Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland. agnieszka.rak@uj.edu.pl
Gregoraszczuk
Ewa Łucja
EŁ
D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D010920:Placenta / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone