Immunohistochemistry of MMPs and TIMPs.
Immunohistochemistry is a useful and powerful method to determine the cells responsible for the production of MMPs and TIMPs and localize them to the tissue areas where they are functioning. This chapter describes the detailed methods of the immunohistochemistry applied to human pathological tissues using commercially available monoclonal antibodies against MMPs and TIMPs. Information about the monoclonal antibodies and solution of the problems with immunostaining is also provided.
Okada
Yasunori
Y
Department of Pathology, School of Medicine, Keio University, Tokyo, Japan.
D001360:Avidin / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D016612:Paraffin Embedding; D009195:Peroxidase / Q000378:metabolism; D016622:Silver Staining; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*
D019714:Tissue Inhibitor of Metalloproteinases; D001360:Avidin; D001710:Biotin; D009195:Peroxidase; D020782:Matrix Metalloproteinases