[Colorectal cancer: tissutal explantation and primary cell culture].
BACKGROUND
Setting of cellular cultures extracted from colorectal cancer tissue represents a valid model for in vitro study of biological and molecular characteristics of each single tumor finalized to obtain a tailored chemiotherapy. The end point of this study is to create primary cellular cultures from "fresh" cancer tissue in different stages of evolution.


METHODS
Cancer tissue samples are obtained by means of surgical excisional biopsy or by means of semi-automatic biopsy instrument (Sprig-Cut). After having compared different approaches, two experimental protocols have been selected to have the highest number or intact cells: enzimatic digestion with trypsin and explantation.


CONCLUSIONS
Primary cell culture free of microbic contamination, obtained mainly by means of Spring-Cut methods, underwent immunohistochemical analysis to evaluate what kind of cell have been grown in vitro by measuring the expression of CK20 and GFAP both resulted positive. The possibility of setting a primary cell culture which represents the cancer of each patient allows a pharmacologic and biomolecular study which can contribute to the development of a tailored adjuvant therapy with many advantages for the patient in terms of positive answer to the treatment and reduced toxicity.
Dipartimento di Chirurgia, Università di Pisa, Italia. r.spisni@dc.med.unipi.it
Roberto
R
Spisni
Alessandra
A
Failli
Giulia
G
Orsini
Olga
O
Kastsiuchenka
Gianfranco
G
Natale
Maura
M
Castagna
Annalisa
A
Legitimo
Alekandr
A
Aghasbabyan
Carlo Enrico
CE
Ambrosini
Rita
R
Consolini
Paolo
P
Miccoli
D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured