Cadaverine turnover in soybean seedlings using 15N-labelled lysine and cadaverine.
The synthesis and translocation of the diamine cadaverine during soybean (Glycine max L. Meer cv. Sakai) germination were studied using 15N-labelled lysine (the cadaverine precursor) and 15N-labelled cadaverine, both under light/dark (12 h/12 h) and total dark germinating conditions. 15N-cadaverine and non-labelled polyamines were simultaneously detected using ionspray ionization-mass spectrometry. Both 15N-cadaverine and 15N-lysine were taken up by soybean. 15N-lysine was transported to the shoot and root and converted into 15N-cadaverine, whereas relatively little 15N-cadaverine was formed from 15N-lysine in the cotyledon. The acropetal translocation of 15N-cadaverine from the cotyledon to the shoot seemed to predominate over basipetal transport to the root. Although no other 15N-derivatised polyamines were found, supplying exogenous 15N-lysine seemed to indirectly affect the metabolism of 14N putrescine, spermidine and spermine, while no significant effect was detected after supplying 15N-cadaverine.
Department of Biochemistry, Dokkyo University School of Medicine, Tochigi, Japan.
Masato
M
Ohe
Hitomi
H
Sasaki
Masaru
M
Niitsu
Nello
N
Bagni
Annalisa
A
Tassoni
Shigeru
S
Matsuzaki
D002103:Cadaverine / Q000378:metabolism*; D018548:Cotyledon; D003624:Darkness; D018525:Germination; D007553:Isotope Labeling; D008027:Light; D008239:Lysine / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism*; D013025:Glycine max / Q000254:growth & development / Q000378:metabolism*
D011073:Polyamines; D008239:Lysine; D002103:Cadaverine