[Effects of respiratory syncytial virus upon expressions of mRNA and protein of intercellular adhesion molecule-1 in human embryonic lung fibroblast cells].
OBJECTIVE
To explore the effects of respiratory syncytial virus (RSV) upon the expressions of mRNA and protein of intercellular adhesion molecule-1 (ICAM-1) in human embryonic lung fibroblast cells.


METHODS
The expressions of mRNA and protein of ICAM-1 were determined in human embryonic lung fibroblast cells after being infected by the LONG strain type A RSV and in normal fibroblast cells with real-time PCR and flow cytometry.


RESULTS
The mRNA of ICAM-1 expression in human embryonic lung fibroblast cells was 2.51 times at 24 h post-infection as that in normal fibroblast cells (P < 0.05). The protein of ICAM-1 expression of RSV control group (1.25 +/- 0.09, 1.87 +/- 0.18, 4.78 +/- 0.52, 13.34 +/- 0.64, 1.58 +/- 0.37) were significantly higher than those of normal cell group (0.21 +/- 0.06, 0.30 +/- 0.06, 0.29 +/- 0.07, 0.35 +/- 0.17, 0.35 +/- 0.14) at 12, 24, 48, 72 and 96 h post-infection (all P < 0.01). The protein of ICAM-1 expression of RSV control group achieved a peak value between 48 h and 72 h, and then it decreased significantly at 96 h.


CONCLUSIONS
Lung fibroblast cell and ICAM-1 may play some roles in pathogenic mechanism of RSV viral pneumonia.
Children's Medical Center, Second Hospital Affiliated to Nanjing Medical University, Nanjing 210011, China. ccxjwxjj@yahoo.com.cn
Cai-xia
CX
Chen
Shou-chuan
SC
Wang
Yu
Y
Hu
Jian-ya
JY
Xu
D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000196:embryology; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism* / Q000821:virology; D012136:Respiratory Syncytial Viruses
D018799:Intercellular Adhesion Molecule-1