Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton-Valentine Leukocidin gene.
In this study, three Panton-Valentine Leukocidin gene carrying methicillin-susceptible Staphylococcus aureus (MSSA) strains (M1-AAG42B, PY30C-b and YF1B-b) were isolated from different food samples in Kesan-Edirne, Turkey. These strains were characterized on the basis of MLST type, spa type, virulence factor gene contents, antibiotic susceptibilities against 21 antibiotics and biofilm formation. The genetic relatedness of the strains was determined by PFGE. In addition, the complete gene sequences of lukS-PV and lukF-PV were also investigated. All strains were found to be susceptible to tested antibiotics and they were mecA negative. Three strains showed the same PFGE band pattern, ST152 clonal type and t355 spa type. In the detection of virulence factor genes, sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, seu, eta, etb, set1, geh and tst genes were not detected. All strains showed the positive results for alpha- and beta-haemolysin genes (hla and hlb), protease encoding genes (sspA, sspB and aur), lukE and lukD leukocidin genes (lukED). The strains were found to be non-biofilm formers. By this study, the virulence properties of the strains were described and this is one of the first reports regarding PVL-positive MSSA strains from food.
Biotechnology and Bioengineering Central Research Laboratory, Izmir Institute of Technology, Gulbahce Campus, Urla, 35430 Izmir, Turkey. msudagidan@yahoo.com.tr
Mert
M
Sudagidan
Ali
A
Aydin
D018829:Adhesins, Bacterial / Q000235:genetics; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D018441:Biofilms; D005098:Exotoxins / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D007956:Leukocidins / Q000235:genetics*; D016106:Methicillin Resistance; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014421:Turkey; D037521:Virulence Factors / Q000235:genetics*
D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D006460:Hemolysin Proteins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C082314:SspA protein, bacteria; D037521:Virulence Factors; C021091:staphylococcal alpha-toxin