Application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue.
The present study demonstrates an application of Parallux (a solid-phase fluorescence immunoassay) for amoxicillin analysis in fish tissue. Amoxicillin at the recommended therapeutic dose (400 mg/kg body weight) was orally administered to three groups of 25 olive flounder ( Paralichthys olivaceus ), 25 rockfish ( Sebastes schlegeli ) and 25 red sea bream ( Pagrus major ) for 7 consecutive days. Amoxicillin was detected in the muscle of fish treated by the 3rd day of the withdrawal period. The recovery rates of all spiked muscle samples were > 86% of the spiked values. The present study showed that solid-phase fluorescence immunoassay can be easily adopted in predicting amoxicillin residues in the muscle tissue of farmed fish.
University of Occupational and Environmental Health Department of Environmental Epidemiology, Institute of Industrial Ecological Sciences Kitahyushu Japan.
Eun-Kee
EK
Park
Won Chul
WC
Jung
Hu-Jang
HJ
Lee
D000658:Amoxicillin / Q000008:administration & dosage / Q000032:analysis*; D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000658:Amoxicillin