A systemic proteomic analysis of Populus chloroplast by using shotgun method.
The chloroplast is one of the most important organelles in plants. Proteomic investigations of chloroplasts have been undertaken for many herb plant species, but to date no such investigation has been reported for woody plant chloroplasts. In the present study we initiated a systematic proteomic study of Populus chloroplasts using a shotgun proteomic method. After isolation of chloroplasts and tryptic digestion of the proteins, the protein fragments were separated via HPLC using an SCX column, and the peptides were analyzed by LC-MS/MS; 119 proteins were successfully identified. Based on annotation information in the UniProtKB/Swiss-Prot database, these proteins were identified as being localized in the chloroplast thylakoid membrane, chloroplast stroma, chloroplast thylakoid lumen, and plastoglobules. Over 50% of all identified proteins were confirmed as chloroplast thylakoid proteins, and 85 are encoded by the chloroplast genome with the remaining proteins encoded by the nuclear genome. Based on functional annotation, these proteins were classified into four functional categories, including photosynthesis, redox regulation and stress, primary and secondary metabolism, transport and signaling. These data provide a valuable basis for further studies on photosynthesis in poplar species.
Yuan
Hong-Mei
HM
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Li
Kai-Long
KL
Ni
Rui-Juan
RJ
Guo
Wen-Dong
WD
Shen
Zhuo
Z
Yang
Chuan-Ping
CP
Wang
Bai-Chen
BC
Liu
Gui-Feng
GF
Guo
Chang-Hong
CH
Jiang
Jing
J
D002736:Chloroplasts / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D016208:Databases, Factual; D057166:High-Throughput Screening Assays / Q000379:methods; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D032107:Populus / Q000737:chemistry* / Q000166:cytology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
D010940:Plant Proteins; D020543:Proteome