The "flipped" state in E71A-K+-channel KcsA exclusively alters the channel gating properties by tetraethylammonium and phosphatidylglycerol.
Mutation E71A in the bacterial K(+)-channel KcsA has been shown to abolish the activation-coupled inactivation of KcsA via significant alterations of the peptide backbone in the vicinity of the selectivity filter. In the present study, we examined channel-blocking behavior of KcsA-E71A by tetraethylammonium (TEA) from both the extra- and the intracellular sides. First, we found that E71A is inserted either in cis or trans orientation in a planar lipid bilayer; however, it exhibits only one orientation in proteoliposomes as determined by extravesicular partial chymotrypsin digestion. Second, E71A exhibits a lower extracellular TEA affinity and is more sensitive to intracellular TEA compared to wild-type KcsA, which apparently has >50-fold higher affinity for extracellular TEA and approximately 2.5-fold lower affinity for intracellular TEA compared to E71A. In additional experiments, we investigated the influence of negatively charged phosphatidylglycerol (PG) on channel-gating properties in phosphatidylcholine lipid bilayers. It was found that high PG content decreases the single-channel conductance and increases the channel open time and open probability. Taken together, our data suggest that the "flipped" conformation of the selectivity filter present in E71A allows weaker extracellular and stronger intracellular TEA binding, whereas higher PG content decreases channel conductivity and stabilizes the channel open "flipped" state via electrostatic interaction in the proximity of the channel pore.
Institute for Biophysics, Johannes Kepler University Linz, 4040 Linz, Austria.
Elisabeth
E
Vales
Mobeen
M
Raja
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli; D015640:Ion Channel Gating / Q000187:drug effects; D008051:Lipid Bilayers / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000378:metabolism / Q000494:pharmacology; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D019789:Tetraethylammonium / Q000378:metabolism / Q000494:pharmacology
D001426:Bacterial Proteins; D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D015221:Potassium Channels; C107790:prokaryotic potassium channel; D019789:Tetraethylammonium