Biodistribution and pharmacokinetics of PEG-10kDa-cholecystokinin-10 in rats after different routes of administration.
Cholecystokinin, produced in the proximal small intestine, is a short acting satiating peptide hormone. CCK-10, before and after mono-iodination, was previously coupled to 10kDa polyethylene glycol (PEG). The formed conjugates PEG10kDa-CCK-10 and PEG10kDa-[(127)I]-CCK-10 show after i.p. administration to rats a sustained food intake reduction during 8h in comparison to 2h for free CCK-10. The present study examined the blood pharmacokinetics of this pharmacological interesting molecule by means of PEG10kDa-[(123)I]-CCK-10 following intravenous, intraperitoneal, intramuscular and nasal administration and the biodistribution after i.p. administration. HPLC analysis with radiometric detection allowed the differentiation between inorganic iodide and the intact tracer in blood. Blood kinetics after i.v. injection was fitted to a bi-exponential with a distribution half-life of 15 min and with an elimination half-life of 8 hours for intact PEG10kDa-[(123)I]-CCK-10. The biodistribution studies showed a higher accumulation of the tracer for all administration routes in organs expressing CCK receptors localized in the gastrointestinal tract such as pancreas, duodenum and small intestine. No indication of blood brain barrier crossing for the conjugate could be observed independently of the administration route. Main clearance was via the urinary pathway.
Laboratory of Toxicology and Bromatology, Faculty of Pharmaceutical Sciences; Katholieke Universiteit Leuven, Leuven, Belgium.
Fabián
F
León-Tamariz
Isabelle
I
Verbaeys
Maurits
M
Van Boven
Marcel
M
De Cuyper
Johan
J
Buyse
Peter
P
de Witte
Alfons
A
Verbruggen
Marnix
M
Cokelaere
D000818:Animals; D002766:Cholecystokinin / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D004333:Drug Administration Routes; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D006207:Half-Life; D007457:Iodine Radioisotopes / Q000097:blood* / Q000652:urine; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D004337:Drug Carriers; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; C050803:cholecystokinin 10 C-terminal fragment; D011092:Polyethylene Glycols; D002766:Cholecystokinin