Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma.
Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79alphacy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gammadelta (TCR-gammadelta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin's lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs.
Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria. barbara.ruetgen@vetmeduni.ac.at
Barbara C
BC
Rütgen
Sabine E
SE
Hammer
Wilhelm
W
Gerner
Maria
M
Christian
Abigail Guija
AG
de Arespacochaga
Michael
M
Willmann
Miriam
M
Kleiter
Ilse
I
Schwendenwein
Armin
A
Saalmüller
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology*; D044963:Biopsy, Fine-Needle; D045744:Cell Line, Tumor / Q000737:chemistry / Q000473:pathology*; D002469:Cell Separation; D002999:Clone Cells / Q000737:chemistry / Q000473:pathology; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005434:Flow Cytometry; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta