The statistically optimized production of poly(gamma-glutamic acid) by batch fermentation of a newly isolated Bacillus subtilis RKY3.
For the production of poly(gamma-glutamic acid), a newly isolated Bacillus sp. RKY3 was phylogenetically identified as Bacillus subtilis based on its 16S rRNA gene sequence. The culture medium for the production of poly(gamma-glutamic acid) by B. subtilis RKY3 was optimized statistically. The parameters significantly affecting poly(gamma-glutamic acid) production were found to be glycerol, glutamic acid, yeast extract, and K(2)HPO(4). A further advanced statistical approach, central composite design, found the optimum levels of the screened variables as follows (gl(-1)): glycerol 17.6, glutamic acid 59.6; yeast extract 2.7; K(2)HPO(4) 2.3. The predicted response as poly(gamma-glutamic acid) production under the statistically optimized conditions was 48.5 g l(-1), which was only 0.4% different from the maximum poly(gamma-glutamic acid) concentration (48.7 g l(-1)) observed at the validation experiment using 7-l lab-scale fermentor containing 3 l of working volume.
School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Jae-Hoon
JH
Jeong
Jin-Nam
JN
Kim
Young-Jung
YJ
Wee
Hwa-Won
HW
Ryu
D000704:Analysis of Variance; D001412:Bacillus subtilis / Q000302:isolation & purification* / Q000378:metabolism*; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D015233:Models, Statistical; D008970:Molecular Weight; D010802:Phylogeny; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D013997:Time Factors; D014783:Viscosity
D012336:RNA, Ribosomal, 16S; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid