Guidelines for reporting of biocatalytic reactions.
Enzymes and whole cells are being increasingly applied in research and industry, but the adoption of biocatalysis relies strongly on useful scientific literature. Unfortunately, too many published papers lack essential information needed to reproduce and understand the results. Here, members of the scientific committee of the European Federation of Biotechnology Section on Applied Biocatalysis (ESAB) provide practical guidelines for reporting experiments. The document embraces the recommendations of the STRENDA initiative (Standards for Reporting Enzymology Data) in the context of pure enzymology and provides further guidelines and explanations on topics of crucial relevance for biocatalysis. In particular, guidelines are given on issues such as the selectivity, specificity, productivity and stability of biocatalysts, as well as on methodological problems related to reactions in multiphase systems. We believe that adoption and use of these guidelines could greatly increase the value and impact of published work in biocatalysis, and hence promote the further growth of applications.
Gardossi
Lucia
L
Dipartimento di Scienze Farmaceutiche, Università di Trieste, Piazzale Europa 1, 34127 Trieste, Italy.
Poulsen
Poul B
PB
Ballesteros
Antonio
A
Hult
Karl
K
Svedas
Vytas K
VK
Vasić-Racki
Durda
D
Carrea
Giacomo
G
Magnusson
Anders
A
Schmid
Andreas
A
Wohlgemuth
Roland
R
Halling
Peter J
PJ
D055162:Biocatalysis; D002477:Cells / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005060:Europe; D011643:Publishing / Q000592:standards*
D004798:Enzymes