Cloning of an orange-spotted grouper Epinephelus coioides heat shock protein 90AB (HSP90AB) and characterization of its expression in response to nodavirus.
The heat shock proteins (HSPs) family which consists of HSP90, HSP70, and low molecular mass HSPs are involved in chaperone activity. Here, we report the cloning and characterization of HSP90AB gene from orange-spotted grouper, Epinephelus coioides. The full-length of grouper HSP90AB was 727 amino acids and possessed an ATPase domain as well as an evolutionarily conserved molecular chaperone. The HSP90AB-green fluorescent protein fusion protein was evenly distributed in the cytoplasm. Immunohistochemistry (IHC) and real-time polymerase chain reaction (PCR) analyses indicated that the expression of grouper HSP90AB was marginally increased following nodavirus infection. Grouper E. coioides that received HSP90 inhibitor geldanamycin (GA) showed an increase in HSP90AB expression and growth of nodavirus supporting nodavirus replication.
Laboratories of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan.
Young-Mao
YM
Chen
Cham-En
CE
Kuo
Ting-Yu
TY
Wang
Pei-Shiuan
PS
Shie
Wei-Chen
WC
Wang
Shao-Ling
SL
Huang
Tieh-Jung
TJ
Tsai
Peng-Peng
PP
Chen
Jiann-Chu
JC
Chen
Tzong-Yueh
TY
Chen
D000595:Amino Acid Sequence; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000276:immunology*; D016227:Benzoquinones / Q000494:pharmacology; D036022:Capsid Proteins / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology*; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D008969:Molecular Sequence Data; D030381:Nodaviridae; D010802:Phylogeny; D012327:RNA Virus Infections / Q000276:immunology / Q000662:veterinary*; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D016227:Benzoquinones; D036022:Capsid Proteins; D006360:Heat-Shock Proteins; D047029:Lactams, Macrocyclic; C001277:geldanamycin