Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos.
In vertebrates, Evx homeodomain transcription factor-encoding genes are expressed in the posterior region during embryonic development, and overexpression experiments have revealed roles in tail development in fish and frogs. We analyzed the molecular mechanisms of posterior neural development and axis formation regulated by eve1. We show that eve1 is involved in establishing trunk and tail neural ectoderm by two independent mechanisms: First, eve1 posteriorizes neural ectoderm via induction of aldh1a2, which encodes an enzyme that synthesizes retinoic acid; second, eve1 is involved in neural induction in the posterior ectoderm by attenuating BMP expression. Further, eve1 can restore trunk neural tube formation in the organizer-deficient ichabod(-/-) mutant. We conclude that eve1 is crucial for the organization of the antero-posterior and dorso-ventral axis in the gastrula ectoderm and also has trunk- and tail-promoting activity.
School of Biosciences, University of Exeter, Devon EX4 4SP, UK.
Carlos
C
Cruz
Shingo
S
Maegawa
Eric S
ES
Weinberg
Stephen W
SW
Wilson
Igor B
IB
Dawid
Tetsuhiro
T
Kudoh
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis; D004475:Ectoderm / Q000196:embryology* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005775:Gastrula / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000096:biosynthesis; D054259:Neural Tube / Q000196:embryology* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013623:Tail / Q000196:embryology*; D014212:Tretinoin / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
D019485:Bone Morphogenetic Proteins; C101121:HOXB1 homeodomain protein; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014212:Tretinoin