Estrogen receptor action in three dimensions - looping the loop.
Due to advances in genomic technologies, our understanding of estrogen receptor (ER)-mediated transcription in breast cancer cells has evolved significantly in recent years. Genome-wide mapping experiments revealed thousands of ER-binding events, but linking them to the target genes has been an ongoing struggle. A recent paper describes a new technique, called ChIA-PET (chromatin interaction analysis using paired-end tag sequencing), that can directly address these questions. ChIA-PET is an unbiased approach for simultaneously identifying all genome-wide binding events of a transcription factor and those involved in long-range chromatin loops.
Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge, CB2 0RE, UK.
Vasiliki
V
Theodorou
Jason S
JS
Carroll
D001943:Breast Neoplasms / Q000235:genetics*; D002843:Chromatin; D005260:Female; D006801:Humans; D011960:Receptors, Estrogen / Q000502:physiology*; D014158:Transcription, Genetic
D002843:Chromatin; D011960:Receptors, Estrogen