Choosing the sequence of dsRNA for RNAi in mouse.
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.
Institute of Molecular Genetics, Academy of Sciences of Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. svobodap@img.cas.cz
Petr
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Svoboda
D020413:3' Untranslated Regions; D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020868:Gene Silencing; D005821:Genetic Techniques; D051379:Mice; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering