Naturally occurring aflatoxin M(1) in raw bulk milk from farm cooling tanks in Argentina.
The aim was to carry out a survey of aflatoxin M(1) (AFM(1)) in raw whole milk from bulk tanks. The sample collection was performed in farms located in one the most important milk-production zones in the centre of Argentina. A total of 94 samples of milk from 47 dairy farms were analysed. AFM(1) analysis involved the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with prior purification of the extracts using immunoaffinity columns. AFM(1) incidence in raw milk was high as 63.8% and levels were between not detected to 0.07 microg l(-1). Several contaminated samples (39%) were over the European Commission limit for infant milk (0.025 microg l(-1)), although none of samples were above Argentine legislation. Estimates of AFM(1) intake were assessed for different age populations. The average AFM(1) estimated daily intakes were 1.6, 0.5, 0.17 ng kg(-1) body weight day(-1) for 4-year-old babies, young children, and adults, respectively. All tested farms used pastures and silages at similar composition. Even though some farms (13) employed high-risk supplementary feeds, such as peanut pod and/or cotton seed, no statistically significant differences were observed between groups. Information from AFM(1) levels in milk in Argentina is limited. A systematic AFM(1) monitoring programme must be performed by means of accurate and reliable analytical techniques as a strategy for protecting milk consumers.
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Cordoba, Argentina.
V A
VA
Alonso
M P
MP
Monge
A
A
Larriestra
A M
AM
Dalcero
L R
LR
Cavaglieri
S M
SM
Chiacchiera
D000328:Adult; D016607:Aflatoxin M1 / Q000737:chemistry*; D000383:Agriculture; D000821:Animal Feed; D000818:Animals; D001118:Argentina; D001835:Body Weight; D002417:Cattle; D002675:Child, Preschool; D005506:Food Contamination; D006801:Humans; D007223:Infant; D008892:Milk / Q000737:chemistry*
D016607:Aflatoxin M1