Beta-diketones: peak shape challenges and the use of mixed-mode high-performance liquid chromatography methodology to obtain fast, high resolution chromatography.
Historically, indirect methods have been used for the HPLC analysis of beta-diketone compounds because of the very poor peak shapes and resolution obtained on conventional HPLC stationary phases. In this paper we demonstrate that it is possible to obtain good peak shapes for underivatised beta-diketone compounds, in a simulated reaction mixture, using only conventional mobile phases with mixed-mode stationary phase HPLC columns. Optimum conditions were obtained using the mixed-mode reversed-phase strong anion exchange column Primesep B, supplied by SIELC Technologies, with a 0.1% aq. TFA/MeOH gradient method and a column temperature of 55 degrees C.
Analytical Research and Development, Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent CT13 9NJ, UK. michael.rance@pfizer.com
M J
MJ
Rance
S
S
Wilson
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D006863:Hydrogen-Ion Concentration; D007659:Ketones / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
D007659:Ketones