Comparison of two different microbiological test kits for detection of periodontal pathogens.
OBJECTIVE
The aim of this study was to compare the outcome of two different microbiological tests for detection of Aggregatibacter actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.), Tannerella forsythia (T.f.) and Treponema denticola (T.d.).


METHODS
A total of 69 adult patients with severe chronic (sCP) or aggressive periodontitis (AgP) participated in the study. Microbiological samples were examined for A.a., P.g., T.f. and T.d. using an RNA probe test (PADO) and a real-time polymerase chain reaction test (MERI).


RESULTS
For all periodontal pathogens under investigation, the median bacterial counts detected with PADO were smaller compared to those detected with MERI. P.g., T.f. and T.d. could be found in the majority of all patients with both tests. With MERI, A.a. was detected more often (24.6%) than with PADO (18.8%). Only 10.1% of the patients tested positive for A.a. with both tests.


CONCLUSIONS
Both tests showed a high percentage of agreement for P.g., T.f. and T.d., but exhibited marked differences in the detection of A.a.
Department of Operative Dentistry, Section of Periodontology, University Hospital Heidelberg, Heidelberg, Germany.
Raluca
R
Cosgarea
Amelie
A
Bäumer
Bernadette
B
Pretzl
Sven
S
Zehaczek
Ti-Sun
TS
Kim
D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D010520:Aggressive Periodontitis / Q000382:microbiology* / Q000628:therapy; D001439:Bacteroides / Q000302:isolation & purification; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000628:therapy; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000517:prevention & control; D005260:Female; D005884:Gingival Hemorrhage / Q000145:classification; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification; D010512:Periodontal Index; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D015347:RNA Probes; D012329:RNA, Bacterial / Q000032:analysis; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D048373:Treponema denticola / Q000302:isolation & purification
D015347:RNA Probes; D012329:RNA, Bacterial; D011933:Reagent Kits, Diagnostic