Lactobacillus-mediated RNA interference in nematode.
We engineered Lactobacillus paracasei to produce a dsRNA that would trigger RNAi-induced silencing of an essential gene in the nematode Caenorhabditis elegans. The dsRNA-expressing L. paracasei can be used in experiments conducted on culture plates and may also be used as an orally administrable dsRNA carrier for humans and other mammals.
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama, Japan.
Ai
A
Kuwahara
Masashi
M
Arita
Akira
A
Kushiro
Yasuji
Y
Sakube
Masahiko
M
Sisido
Takashi
T
Ohtsuki
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D006801:Humans; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism; D009348:Nematoda / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction