Fluorescent structural DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates.
Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.
LI-COR Biosciences, Inc., 4647 Superior Street, Lincoln, Nebraska 68504, USA. jon.anderson@licor.com
Jon P
JP
Anderson
Bambi L
BL
Reynolds
Kristin
K
Baum
John G
JG
Williams
D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry*; D010316:Particle Size; D010710:Phosphates / Q000737:chemistry
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D009711:Nucleotides; D010710:Phosphates; D004247:DNA