Synthetic surfaces for human embryonic stem cell culture.
Human embryonic stem cells (hESCs) have numerous potential biomedical applications owing to their unique abilities for self-renewal and pluripotency. Successful clinical application of hESCs and derivatives necessitates the culture of these cells in a fully defined environment. We have developed a novel peptide-based surface that uses a high-affinity cyclic RGD peptide for culture of hESCs under chemically defined conditions.
Kolhar
Poornima
P
Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106, USA.
Kotamraju
Venkata Ramana
VR
Hikita
Sherry T
ST
Clegg
Dennis O
DO
Ruoslahti
Erkki
E
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid