Quick PCR based diagnosis of typhoid using specific genetic markers.
A quick multiplex PCR based detection method was developed for early diagnosis of typhoid using specific genetic markers of S. typhi. Primers of tyv gene, flag gene, viaB gene and ratA gene confirmed the specificity and sensitivity of the PCR. The serum samples of the suspected typhoid patients were taken directly for PCR without culturing and isolating genomic DNA. Overall diagnosis required 2 h which is the least time ever reported for a PCR based methods. The sensitivity of the method is up to 5 fg genomic DNA. The genetic markers are specific and the four pairs of primers give selective amplification and differentiate S. typhi from closely related S. typhimurium.
Kumar
Ashok
A
Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India. ashokigib@rediffmail.com
Balachandran
Yadu
Y
Gupta
Sunil
S
Khare
Shashi
S
Suman
D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014435:Typhoid Fever / Q000175:diagnosis*
D001426:Bacterial Proteins; D017931:DNA Primers; D005819:Genetic Markers