Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia.
Maternal smoking during pregnancy increases the incidence and severity of respiratory infections in neonates. Surfactant proteins A and D (SP-A and SP-D, respectively) are components of pulmonary innate immunity and have an important role in defense against inhaled pathogens. The purpose of this study was to determine if nicotine exposure during the third trimester of pregnancy alters the expression of SP-A and SP-D of fetal lung epithelia. Pregnant ewes were assigned to four groups; a nicotine-exposed full-term and pre-term group, and control full-term and pre-term group. Lung tissue was collected for Western blot and IHC analysis of SP-A level, Western blot analysis of SP-D level and qPCR analysis of SP-A and SP-D mRNA expression. Exposure to nicotine significantly decreased SP-A gene expression (P = 0.01) and SP-A protein level in pre-term lambs. This finding suggests that maternal nicotine exposure during the last trimester of pregnancy alters a key component of lung innate immunity in offspring.
Lazic
Tatjana
T
Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA. tlazic@iastate.edu
Matic
Milan
M
Gallup
Jack M
JM
Van Geelen
Albert
A
Meyerholz
David K
DK
Grubor
Branka
B
Imerman
Paula M
PM
de-Macedo
Marcia M M A
MM
Ackermann
Mark R
MR
D000279:Administration, Cutaneous; D000818:Animals; D015153:Blotting, Western; D003367:Cotinine / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005325:Fetal Organ Maturity / Q000187:drug effects*; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology*; D009538:Nicotine / Q000008:administration & dosage / Q000009:adverse effects*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism*; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012756:Sheep; D012907:Smoking / Q000009:adverse effects
D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D012333:RNA, Messenger; D009538:Nicotine; D003367:Cotinine