In situ hybridization for metalloproteinases and their inhibitors.
In situ hybridization (ISH) is an invaluable tool in understanding tissue-specific gene expression and gene regulation within a spatial context and at a resolution that is not possible by any other method. In this chapter, we provide ISH methodology that has successfully been applied to the detection of metalloproteinases and their inhibitors.
Hurskainen
Tiina L
TL
Clinical Research Center, Oulu University Hospital, Oulu, Finland.
Apte
Suneel S
SS
D000818:Animals; D011232:Chemical Precipitation; D016616:Cryoultramicrotomy; D018076:DNA, Complementary / Q000235:genetics; D004076:Digoxigenin / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D000431:Ethanol; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D015347:RNA Probes / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013194:Staining and Labeling; D013698:Templates, Genetic; D016707:Tissue Fixation; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D015347:RNA Probes; D000431:Ethanol; D012260:Ribonucleases; D045726:Metalloproteases; D004076:Digoxigenin