Evaluation of the automated system Vitek2 for identification and antimicrobial susceptibility testing of Brazilian Gram-positive cocci strains.
Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance of this equipment for Brazilian isolates that had been characterized using reference identification and antimicrobial susceptibility testing methods. Ninety-nine strains of Gram-positive cocci from a local reference center collection were analyzed, consisting of 50 coagulase-negative Staphylococcus (CoNS) and 49 Enterococcus and related species. Vitek2 correctly identified 79.8% (79/99) of the isolates. Oxacillin resistance was detected in 76% (19/25) of resistant S. epidermidis strains and in 88% (22/25) of other resistant CoNS species strains. Vancomycin resistance was detected in 100% (20/20) of resistant Enterococcus and related species strains. Vitek2 performed very well for the identification of S. epidermidis and non-epidermidis staphylococci, and for the detection of vancomycin resistance in Enterococcus and related species. However, the system needs improvement in order to provide reliable results for the characterization of some CoNS species, identification of Enterococcus and related species and for detecting oxacillin resistance in CoNS.
d'Azevedo
Pedro Alves
PA
Department of Microbiology and Parasitology, Clinical Medical School of Porto Alegre, Rua Sarmento Leite 245/204, Porto Alegre, RS, Brazil. pedroaze@ufcspa.edu.br
Siquiera
Itacy
I
Gugel
Juliana
J
Antunes
Ana Lúcia Souza
AL
Secchi
Carina
C
Pasternak
Jacyr
J
Martino
Marinês Dalla Vale
MD
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D057205:Automation, Laboratory / Q000379:methods*; D016983:Enterococcus / Q000145:classification / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
D000900:Anti-Bacterial Agents