Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion.
The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.
Advanced Research Center, School of Life Dentistry at Niigata, Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. aoy@ngt.ndu.ac.jp
Hidekazu
H
Aoyagi
Kohzo
K
Tsuchikawa
Shin-ichi
S
Iwasaki
D019412:Anatomy, Cross-Sectional / Q000379:methods; D000818:Animals; D001808:Blood Vessels / Q000196:embryology; D004622:Embryo, Mammalian / Q000033:anatomy & histology*; D047108:Embryonic Development; D047109:Fetal Development; D021621:Imaging, Three-Dimensional / Q000662:veterinary*; D051379:Mice; D038081:Organogenesis; D014278:Trigeminal Nuclei / Q000033:anatomy & histology / Q000196:embryology*; D055114:X-Ray Microtomography / Q000662:veterinary*