Increased FKBP51 in induced sputum cells of chronic obstructive pulmonary disease patients after therapy.
OBJECTIVE
Immunophilin FKBP51 assists polypeptide folding, participates in glucocorticoid actions and may play a role in glucocorticoid resistance. FKBP51 is altered in patients with asthma, but its role in chronic obstructive pulmonary disease (COPD) characterized by dysregulation of several pro/antiinflammatory genes is less clear.


METHODS
We assessed changes in nuclear/cytosolic FKBP51 protein using SDS-PAGE/WB and FKBP51 mRNA by qRT-PCR in cells isolated from induced sputum of stable COPD patients treated with formoterol/budesonide or formoterol/budesonide/theo?phylline for 4 wk.


RESULTS
Expression of FKBP51 was higher in formoterol/ budesonide/theophylline-treated patients, compared with formoterol/budesonide group in both cytosolic and nuclear fractions by about 57% and 31%, respectively (P<0.001, P<0.01). FKBP51 mRNA was only slightly, but not significantly, higher in patients on formoterol/ budesonide/theophylline.


CONCLUSIONS
Increased FKBP51 in COPD patients treated with formoterol/ budesonide/theophylline may be important in altering signaling from corticosteroid receptors.
Department of Clinical Pharmacology, Medical University of Bialystok, Poland. Holow_sinai@hotmail.com
A
A
Holownia
R M
RM
Mroz
A
A
Kolodziejczyk
E
E
Chyczewska
J J
JJ
Braszko
D019819:Budesonide / Q000008:administration & dosage; D004359:Drug Therapy, Combination; D004983:Ethanolamines / Q000008:administration & dosage; D000068759:Formoterol Fumarate; D006801:Humans; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D013183:Sputum / Q000737:chemistry*; D022021:Tacrolimus Binding Proteins / Q000032:analysis* / Q000235:genetics; D013806:Theophylline / Q000008:administration & dosage; D000099159:Tacrolimus Binding Protein 5
D019819:Budesonide; D004983:Ethanolamines; D000068759:Formoterol Fumarate; D012333:RNA, Messenger; D022021:Tacrolimus Binding Proteins; D013806:Theophylline; D000099159:Tacrolimus Binding Protein 5