Chromium(VI) is more toxic than chromium(III) to freshwater algae: a paradigm to revise?
The behavior and toxicity of Cr(III) and Cr(VI) to the green algae Pseudokirchneriella subcapitata and Chlorella kessleri were studied in a standard culture medium (ISO medium) and, for P. subcapitata only, in ultrafiltered natural water enriched with all ISO components (modified ISO medium). In all solutions amended with Cr(III), initial chromium concentrations decreased by 60-90% over 72h (the duration of algal tests) indicating that protocols for testing poorly soluble substances are required to properly evaluate Cr(III) toxicity. After accounting for its behavior in test solutions, chromium(III) was 5-10 times more toxic than Cr(VI) in both media. For P. subcapitata, the average 72h EC50 of Cr(III) in ISO medium was 17.4+/-4.7 microg/L (n=9); lower than corresponding hardness-corrected Continuous Concentration Criteria of the US EPA and well within the range of Cr concentrations found in waters impacted by tannery discharges. These results follow from intrinsic chemical properties of Cr(III) in circumneutral solutions, so that the actual toxicity of Cr(III) to aquatic organisms may be generally underestimated.
Vignati
Davide A L
DA
Institut F-A Forel, CP 416, 1290 Versoix, Switzerland. vignati@irsa.cnr.it
Dominik
Janusz
J
Beye
Mamadou L
ML
Pettine
Maurizio
M
Ferrari
Benoît J D
BJ
D000460:Chlorophyta / Q000187:drug effects*; D002857:Chromium / Q000633:toxicity*; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion