Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles.
Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
Ghosh
Partha
P
Department of Chemistry, University of Massachusetts at Amherst, Amherst, Massachusetts 01003, USA.
Yang
Xiaochao
X
Arvizo
Rochelle
R
Zhu
Zheng-Jiang
ZJ
Agasti
Sarit S
SS
Mo
Zhihong
Z
Rotello
Vincent M
VM
D000818:Animals; D016923:Cell Death; D002460:Cell Line; D002463:Cell Membrane Permeability; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000008:administration & dosage* / Q000378:metabolism
D010455:Peptides; D006046:Gold; D001616:beta-Galactosidase