[Application of interphase FISH on cell smears in detection of hematological diseases].
The study was aimed to investigate the application value of interphase fluorescence in situ hybridization (FISH) on cell smears in hematological diseases. Both interphase FISH on peripheral blood smears and bone marrow smears treated by methanol/acetic acid, and routine interphase FISH of bone marrow cells dropped on slides were done at the same time, in order to detect Ph chromosome by BCR/ABL dual color, dual fusion probe in 20 patients with chronic myelogenous leukemia or acute lymphoblastic leukemia which had been proven to display Ph chromosome positive. The results indicated that as compared with routine interphase FISH, the interphase FISH on cell smears could also offer reliable result. It is concluded that interphase FISH on cell smears is a kind of reliable and time-saving technique, which is also suitable for retrospective research and worthy to further apply in clinic.
Sun
Wan-Ling
WL
Department of Hematology, Xuanwu Hosptial, Capital Medical University, Beijing 100053, China.
Liu
Cong-Yan
CY
Li
Hui
H
He
Jing-Juan
JJ
Sun
Xue-Jing
XJ
Xu
Juan
J
D000328:Adult; D000368:Aged; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006402:Hematologic Diseases / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D008297:Male; D008875:Middle Aged; D055815:Young Adult