Comparison of HPLC and CZE methods for analysis of DOTA-like esters--reaction intermediates in synthesis of magnetic resonance contrast agents.
RP-HPLC and non-aqueous CZE methods were evaluated for qualitative and quantitative analysis of esterified macrocyclic compounds of the DOTA family (DOTA=1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid). This group of compounds represents important reaction intermediates in synthesis of magnetic resonance contrast agents. Calibration curves in the concentration range from 1.0 x 10(-5) to 1.0 x 10(-3) mol/L were plotted and the experimental data were critically compared in terms of their repeatability, linearity, LOD and LOQ. The optimized methods were successfully applied to the analysis of a real reaction mixture without any further pretreatment.
Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov, Prague, Czech Republic.
Anna
A
Hamplová
Tomás
T
Krízek
Vojtech
V
Kubícek
Zuzana
Z
Bosáková
Pavel
P
Coufal
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D004952:Esters / Q000032:analysis* / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
D003287:Contrast Media; D004952:Esters; D006573:Heterocyclic Compounds, 1-Ring; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid