Comparative cytotoxicity of five root canal sealers on cultured human periodontal ligament fibroblasts.
OBJECTIVE
To evaluate the cytotoxicity of current root canal sealers to periodontal ligament (PDL) fibroblasts.


METHODS
Five root canal sealers (Canals, Canals-N, Topseal, Sealapex, Tubliseal) were prepared and placed into transwells. After initial setting for 1 h, the transwells with sealers were placed into cultured PDL fibroblasts. They were cultured for further 3 or 18 h. Morphological changes were observed. Cell viability was estimated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5- diphenyl-tetrazolium bromide (MTT) assay.


RESULTS
Marked retraction and death of PDL fibroblasts were observed after exposure to Canals or Topseal for 3 h. A 3-h exposure of PDL fibroblasts to Tubliseal stimulated MTT reduction. Canals-N showed little cytotoxicity even after an exposure of 18 h.


CONCLUSIONS
Canals was the most toxic sealer, followed by Topseal. Sealapex and Tubliseal had comparable and moderate cytotoxicity to PDL fibroblasts, whereas Canals-N showed little cytotoxicity. Exposure to Tubliseal may modulate MTT reduction in PDL fibroblasts. Canals-N had good biocompatibility.
Biomedical Science Team, Chang Gung Institute of Technology, Taoyuan, Taiwan.
M C
MC
Chang
L D
LD
Lin
Y J
YJ
Chen
Y L
YL
Tsai
Y A
YA
Cheng
C S
CS
Kuo
H H
HH
Chang
T F
TF
Tai
H J
HJ
Lin
J H
JH
Jeng
D000704:Analysis of Variance; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D018709:Statistics, Nonparametric
D012387:Root Canal Filling Materials