Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression.
BACKGROUND
MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored.


RESULTS
Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter.


CONCLUSIONS
We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1beta stimulation.
Kasza
Aneta
A
Dept of Cell Biochemistry, Jagiellonian University, Krakow, Poland. aneta.kasza@uj.edu.pl
Wyrzykowska
Paulina
P
Horwacik
Irena
I
Tymoszuk
Piotr
P
Mizgalska
Danuta
D
Palmer
Karren
K
Rokita
Hanna
H
Sharrocks
Andrew D
AD
Jura
Jolanta
J
D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D056945:Hep G2 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012260:Ribonucleases; D026362:Serum Response Factor / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D050807:ets-Domain Protein Elk-1 / Q000378:metabolism*
D053583:Interleukin-1beta; D016328:NF-kappa B; D026362:Serum Response Factor; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D012260:Ribonucleases; C510646:ZC3H12A protein, human