The effect of Xuefu Zhuyu Decoction on in vitro endothelial progenitor cell tube formation.
OBJECTIVE
To observe the effect of Xuefu Zhuyu Decoction ()-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.


METHODS
Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.


RESULTS
After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.


CONCLUSIONS
XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.
Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China.
Dong
D
Gao
Li-ya
LY
Wu
Yu-huan
YH
Jiao
Wen-yuan
WY
Chen
Yan
Y
Chen
Ted J
TJ
Kaptchuk
Bo
B
Lu
Jun
J
Song
Ke-ji
KJ
Chen
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction