DNA-hydrolyzing activity of IgG antibodies from the sera of patients with tick-borne encephalitis.
DNase autoantibodies (Abzs) can be found in the blood of patients with several autoimmune diseases, while the blood of healthy donors or patients with diseases with an insignificant disturbance of the immune status does not contain DNase Abzs. Here we present the first analysis of the DNase Abzs activity in the patients with tick-borne encephalitis (TBE). Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of TBE patients but not from healthy donors. The relative activity of IgGs has been shown to vary extensively from patient to patient, but most of the preparations (91%) had detectable levels of the DNase activity. Polyclonal DNase IgGs were not active in the presence of EDTA or after a dialysis against EDTA, but could be activated by several externally added metal ions, with the level of activity decreasing in the order Mn(2+) + Ca(2+) > or = Mn(2+)+ Mg(2+) > or = Mn(2+) > or = Mg(2+) + Ca(2+) > or = Co(2+) > or = Mg(2+) > Ca(2+), while K(+), Na(+), Ni(2+), Zn(2+), and Cu(2+) did not stimulate DNA hydrolysis. Affinity chromatography on DNA-cellulose separated the DNase IgGs into many subfractions with various affinities for DNA and very different levels of the relative activity. Possible reasons for catalytic diversity of polyclonal human Abzs are discussed.
Parkhomenko
Taisiya A
TA
Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Division, Novosibirsk 630090, Russia.
Buneva
Valentina N
VN
Tyshkevich
Ol'ga B
OB
Generalov
Igor I
II
Doronin
Boris M
BM
Nevinsky
Georgy A
GA
D002853:Chromatography, Liquid; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004675:Encephalitis, Tick-Borne / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification; D008670:Metals / Q000378:metabolism
D007074:Immunoglobulin G; D008670:Metals; D004247:DNA