Overexpression of gamma-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: physiological and chlorophyll a fluorescence measurements.
Tocopherols (vitamin E) are lipid soluble antioxidants synthesized by plants and some cyanobacteria. We have earlier reported that overexpression of the gamma-tocopherol methyl transferase (gamma-TMT) gene from Arabidopsis thaliana in transgenic Brassica juncea plants resulted in an over six-fold increase in the level of alpha-tocopherol, the most active form of all the tocopherols. Tocopherol levels have been shown to increase in response to a variety of abiotic stresses. In the present study on Brassica juncea, we found that salt, heavy metal and osmotic stress induced an increase in the total tocopherol levels. Measurements of seed germination, shoot growth and leaf disc senescence showed that transgenic Brassica juncea plants overexpressing the gamma-TMT gene had enhanced tolerance to the induced stresses. Analysis of the chlorophyll a fluorescence rise kinetics, from the initial "O" level to the "P" (the peak) level, showed that there were differential effects of the applied stresses on different sites of the photosynthetic machinery; further, these effects were alleviated in the transgenic (line 16.1) Brassica juncea plants. We show that alpha-tocopherol plays an important role in the alleviation of stress induced by salt, heavy metal and osmoticum in Brassica juncea.
Yusuf
Mohd Aslam
MA
School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Kumar
Deepak
D
Rajwanshi
Ravi
R
Strasser
Reto Jörg
RJ
Tsimilli-Michael
Merope
M
Govindjee
Sarin
Neera Bhalla
NB
D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D005453:Fluorescence; D018525:Germination; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology*; D009149:Mustard Plant / Q000378:metabolism*; D010788:Photosynthesis; D030821:Plants, Genetically Modified / Q000378:metabolism*; D013312:Stress, Physiological
D002734:Chlorophyll; D008780:Methyltransferases; C047259:gamma-tocopherol methyltransferase; D000077194:Chlorophyll A