Multicopy suppression of oxidant-sensitive eos1 mutation by IZH2 in Saccharomyces cerevisiae and the involvement of Eos1 in zinc homeostasis.
EOS1 is required for tolerance to oxidative stress in Saccharomyces cerevisiae; mutants are defective in the gene sensitive to hydrogen peroxide and tolerant to tunicamycin. To clarify the function of Eos1, we screened yeast genomic DNA libraries for heterologous genes that, when overexpressed from a plasmid, can suppress the hydrogen peroxide-sensitive eos1 mutation. We identified one such gene, IZH2, which has previously been reported to be a Zap1-regulated gene. However, the EOS1 and IZH2 genes do not themselves appear to be functionally interchangeable. Double disruption of the EOS1 and IZH2 genes yielded a slow-growth phenotype, suggesting that the two proteins are involved in related cellular processes. DNA microarray analysis revealed decreased expression of Zap1-regulated genes in the eos1-deletion mutant (Deltaeos1). Thus, it is likely that Eos1 is involved in zinc homeostasis.
National Food Research Institute, Ibaraki, Japan.
Toshihide
T
Nakamura
Shunsuke
S
Takahashi
Hiroshi
H
Takagi
Jun
J
Shima
D017353:Gene Deletion; D018628:Gene Dosage; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000633:toxicity; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D016877:Oxidants / Q000633:toxicity; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015032:Zinc / Q000378:metabolism*
C517528:EOS1 protein, S cerevisiae; C497767:IZH2 protein, S cerevisiae; D008565:Membrane Proteins; D016877:Oxidants; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D015032:Zinc