Thiol-sensitive mutant forms of human SOD2, L60F, and I58T: the role of Cys140.
We previously described a coding mutation (L60F) in the mitochondrial superoxide dismutase (SOD2) gene of the human T cell leukemia-derived cell line Jurkat. In cell extracts the L60F mutant enzyme showed unusual inhibition by thiol reagents not seen in wild-type enzyme. Here we compare the properties of purified recombinant L60F SOD2 with a previously described SOD2 mutant, I58T. Both mutant proteins display a weakened dimer-dimer interaction and thermal instability at 55 degrees C. Both I58T and L60F lose activity at 37 degrees C in the presence of 5 mM N-ethylmaleimide, whereas the wild-type SOD2 does not. Each subunit contains one exposed, reactive cysteine residue at position 196 and a second cysteine residue at 140, which is buried and unreactive in the wild-type tetramer. We propose that the mutant enzymes, which exist largely as dimers, allow both cysteine residues to react with thiol reagents. When the cysteine residue at 140 was changed to serine by site-directed mutagenesis, both double mutants I58T/C140S and L60F/C140S lost their increased thiol sensitivity. The evolutionary significance of Cys140 is discussed.
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Colorado at Denver, Aurora, CO 80045, USA.
Daniel
D
Hernandez-Saavedra
Celia
C
Quijano
Verónica
V
Demicheli
José M
JM
Souza
Rafael
R
Radi
Joe M
JM
McCord
D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000099156:Superoxide Dismutase 2
D003545:Cysteine; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2