Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood.
The conditions for cryopreservation of CD34(+) hematopoietic stem cells (HSC) from umbilical cord blood (UCB) were optimized with a new cryo-medium containing 10% ethylene glycol (EG) and 2% dimethyl sulfoxide (Me(2)SO) using a controlled-rate freezing (CRF) method. After the cryopreservation of mononuclear cells (MNC) from UCB, recoveries of MNC, CD34(+) cells, and total colony-forming units (CFU) were significantly improved compared to those in the control cryo-medium containing 10% Me(2)SO and 2% Dextran-40 (P<0.05). This study shows that the new cryo-medium and CRF method provide better recoveries of MNC, HSC and total CFU than the control cryo-medium and isopropylalcohol freezing (IPA) method. Therefore, this cryo-medium, combined with the CRF method, is valuable for optimizing cryopreservation conditions for HSC from UCB to obtain satisfactory HSC recovery.
Son
Jeong Hwa
JH
Binex Research Institute, Binex Co. Ltd., 480-2, Jangrim-dong, Saha-gu, Busan 604-846, Republic of Korea. sjhwa@bi-nex.com
Heo
Yoon Jeong
YJ
Park
Mi Young
MY
Kim
Hyung Hoi
HH
Lee
Kyu Sup
KS
D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005312:Fetal Blood; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D006801:Humans
D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide