[Effect of alpha-lipoic acid on fibroblasts 3T3 and 3T3-SV40. Comparison with N-acetylcysteine action].
In this study we investigated the effect of alpha-lipoic acid (ALA) in concentration range 0.7-5.0 mM on the intracellular level of reduced glutathione, the cell cycle phase distribution, the structure of microfilaments and microtubules of normal (3T3) and transformed (3T3-SV40) fibroblasts. We obtained that ALA increased the glutathione content in transformed cells, but did not change its level in normal cells, induced cell cycle arrest of 3T3 cells (but not 3T3-SV40 cells), and disrupted actin microfilaments in cells of both lines. The effect of ALA was compared with N-acetylcysteine (NAC) action. The whole complex of findings allows us to affirm that each of these antioxidants acts on its own target molecules in normal and transformed cells and activates different signal and metabolic pathways in these cells. But at the same time the intermediate steps of ALA and NAC action can be common (alteration of the intracellular level of glutathione, reorganization of actin cytoskeleton, etc.).
Vakhromova
E A
EA
Polozov
Iu S
IuS
Kirpichnikova
K M
KM
Aksenov
N D
ND
Gamaleĭ
I A
IA
D016475:3T3 Cells; D000111:Acetylcysteine / Q000494:pharmacology*; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D013539:Simian virus 40; D008063:Thioctic Acid / Q000494:pharmacology*
D000975:Antioxidants; D016166:Free Radical Scavengers; D008063:Thioctic Acid; D005978:Glutathione; D000111:Acetylcysteine