[FBAT analysis of polymorphisms of vitamin D receptor (Taq I and Fok I) in aggressive periodontitis families].
OBJECTIVE
To analyze the correlation between polymorphisms of the vitamin D receptor gene (Taq I and Fok I) and the aggressive periodontitis by FBAT method.


METHODS
93 AgP nuclear families including 93 probands and their 155 relatives were recruited. The genotype frequency and polymorphism for VDR for the patients and their pedigree were detected by using polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP).


RESULTS
The frequency of allele T and t accounted for 94.6 % and 5.4 % in the total populations. No tt genotype were detected. The fathers of probands carried more allele t than the mothers(9.8% vs 1.6%, P=0.005). The frequency of allele F and f accounted for 57.1 % and 42.9 % in the total populations. The result of family based associated test (FBAT) including additive model, dominant model and recessive model showed that different alleles of Taq I and Fok I had no correlation with the onset of AgP(P>0.05).


CONCLUSIONS
This was the first time to detect the VDR gene polymorphisms in Chinese families. The result of FBAT analysis can not show the correlation between vitamin D receptor gene polymorphisms (Taq I and Fok I) and the onset of AgP.
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Yu
Y
Tian
Li
L
Xu
Huan Xin
HX
Meng
Xiu Yun
XY
Ren
Zhi Bin
ZB
Chen
Li
L
Zhang
Kai Ning
KN
Liu
D010520:Aggressive Periodontitis / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*; D019914:Taq Polymerase / Q000235:genetics
D018167:Receptors, Calcitriol; D019914:Taq Polymerase; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific