Immunodiagnosis of Chagas disease: Synthesis of three latex-protein complexes containing different antigens of Trypanosoma cruzi.
This article describes the physical adsorption and the chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes to be used as novel immunodiagnosis reagents for detecting the Chagas disease. The coupled proteins were a homogenate of T. cruzi, or a recombinant protein (either Ag36 or CP1). With the homogenate, between 30 and 60% of the total-linked protein was chemically coupled, showing a small dependence with the pH. For Ag36 and CP1, around 90% of the total-linked protein was chemically coupled, with a maximum coupling at pH 5 (i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36.
Gonzalez
Verónica D G
VD
INTEC, Universidad Nacional del Litoral and CONICET, Güemes, Santa Fe, Argentina.
Garcia
Valeria S
VS
Vega
Jorge R
JR
Marcipar
Ivan S
IS
Meira
Gregorio R
GR
Gugliotta
Luis M
LM
D000953:Antigens, Protozoan / Q000032:analysis*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007840:Latex / Q000737:chemistry*; D011994:Recombinant Proteins / Q000138:chemical synthesis; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D007840:Latex; D011994:Recombinant Proteins