Real time analysis of neurotransmitters in the brain using a micro-electrode system.
Neurotransmitters catechol and dopamine analogy levels are related to the systematic nerve activity. This assay was performed using cyclic voltammetry (CV) and square wave (SW) stripping voltammetry, while optimization was performed by the carbon fiber microelectrode (CFME) with wire-type reference and counter electrodes. In the electrolyte solutions, the analytical parameters of catechol were as follows: 8.5 pH, -0.4 V anodic, 0.3 V cathodic, 15s deposition time, 15 Hz SW frequency, 25 mV SW amplitude and incremental potential of 4 mV. Under optimal conditions, working ranges of 0-50 mgL(-1) CV, 0.01-50 mgL(-1) cathodic, 1-13 mgL(-1) anodic. The relative standard deviation of 0.02 %, n = 15 using 0.5 mgL(-1) standard. The detection limit was 4.0 ugL(-1). It can be used for in vivo direct analysis. Here, diagnostic applications were performed in the brain skeleton of living cells in real time. The developed techniques can be useful for nerve control and signal detections.
Ly
Suw Young
SY
Biosensor Research Institute, Seoul National University of Technology, 172, gongreung 2-dong Nowon-gu, Seoul,139-743, South Korea. suwyoung@snut.ac.kr
Heo
Hwa Jin
HJ
Kim
Min Jung
MJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008839:Microelectrodes; D018377:Neurotransmitter Agents / Q000378:metabolism*; D013997:Time Factors
D018377:Neurotransmitter Agents