[Reconstruction of osteochondral defects with a stem cell-based cartilage-polymer construct in a small animal model].
OBJECTIVE
Mesenchymal stem cells have a high therapeutic potential for the reconstruction of articular cartilage defects. In this study, a cartilage-polymer construct using mesenchymal stem cells from trabecular bone and a polylactic acid polymer was fabricated with a press-coating technique. We investigated whether cells from human trabecular bone fragments have the same chondrogenic differentiation potential as mesenchymal stem cells derived from bone marrow and whether it is possible to reconstruct an osteochondral lesion in the nude rat with the fabricated construct.


METHODS
Cells were obtained from the femoral head of patients undergoing total hip arthroplasty. The fabrication of the constructs was performed by centrifugation of 1.5x10(6) cells to a cell pellet which was then placed in a polymer block. The fabricated cell constructs were cultivated for 3 weeks in a serum-free medium, supplemented with transforming growth factor beta1. Every third day, the chondrogenic differentiation was analysed using chondrogenic and osteogenic marker genes. After three weeks the constructs were implanted into 5 mm osteochondral defects of the knee joint of nude rats. After 4 and 12 weeks histochemical and immunohistochemical analyses were performed.


RESULTS
At the end of the culture period the constructs showed a proteoglycan-rich extracellular matrix with the expression of collagen types II, IX and X as well as aggrecan und COMP (cartilage oligomeric matrix protein). No osteogenic markers except collagen type I could be detected. The analysis of the in vivo experiment showed a good defect filling with a reconstructed cartilage surface along with increasing resorption of the polymer.


CONCLUSIONS
We have shown that it is possible to fabricate cartilage-polymer constructs from trabecular bone-derived cells, and that the cells have the same chondrogenic differentiation potential as mesenchymal stem cells derived from bone marrow. With the fabricated cartilage-polymer construct it is possible to reconstruct an osteochondral defect in the knee joint of the nude rat.
Abteilung für Unfallchirurgie, Klinikum der Universität Regensburg, Regensburg. arne.berner@klinik.uni-regensburg.de
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Berner
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Siebenlist
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Reichert
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Hendrich
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Nöth
D000818:Animals; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology*; D001842:Bone and Bones / Q000601:surgery*; D002454:Cell Differentiation / Q000235:genetics; D019902:Chondrocytes / Q000166:cytology* / Q000637:transplantation*; D020219:Chondrogenesis / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D005819:Genetic Markers / Q000235:genetics; D007719:Knee Joint / Q000473:pathology / Q000601:surgery; D019344:Lactic Acid; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011091:Polyesters; D011108:Polymers; D019736:Prostheses and Implants; D051381:Rats; D011923:Rats, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
D005819:Genetic Markers; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)