Determination of Citrus aurantium protoalkaloids using HPLC with acidic potassium permanganate chemiluminescence detection.
Acidic potassium permanganate chemiluminescence was explored as a sensitive and selective mode of detection for phenolic phenethylamines (adrenergic amines) in consumer products containing Citrus aurantium extracts. Nine commercially available weight-loss products were analysed using rapid reversed-phase chromatography with a monolithic column (separation time of 4 min). The results were in good agreement with package labelling, with some notable exceptions. The products contained a wide concentration range of synephrine and total adrenergic amines, and the difference in consumer intake was even greater when the manufacturers' recommended daily consumption was considered. The quantity of the extract, often specified on the packaging as equivalent grams of dry C. aurantium fruit, was a poor indicator of the concentration of the active ingredients. Methionine, a thioether amino acid contained in some weight-loss products, was identified as a potential interferent for this mode of detection.
School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
Dane W
DW
Percy
Jacqui L
JL
Adcock
Xavier A
XA
Conlan
Neil W
NW
Barnett
Michelle E
ME
Gange
Laura K
LK
Noonan
Luke C
LC
Henderson
Paul S
PS
Francis
D000470:Alkaloids / Q000032:analysis*; D019440:Anti-Obesity Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D010627:Phenethylamines / Q000032:analysis*; D028321:Plant Preparations / Q000032:analysis*; D011196:Potassium Permanganate / Q000737:chemistry; D012680:Sensitivity and Specificity
D000470:Alkaloids; D019440:Anti-Obesity Agents; D010627:Phenethylamines; D028321:Plant Preparations; D011196:Potassium Permanganate