The nongenomic effects of progesterone in repressing iNOS activation through P38MAPK pathways in gonococci-infected polymorphonuclear leukocytes and the clinical significance.
Progesterone has nongenomic effects on inducible nitric oxide synthase (iNOS), which is mediated by mitogen activated protein kinase (MAPK) pathways. This effect is supposed to have some potential association with asymptomatic gonococcal infections in women by immunological depression. In this study, polymorphonuclear leukocytes (PMNs) challenged by gonococci were used to study the nongenomic effects of progesterone. The activation of iNOS was assessed by measuring [(3)H] L-arginine converses to [(3)H] L-citrulline, and the activity of MAPK was detected by Western blot. It was found that the activity of iNOS and the yields of NO were enhanced significantly in gonococci-challenged PMNs compared with the controls (P<0.01). Progesterone could repress the activation of iNOS through P38MAPK pathway within PMNs (P<0.05), which could be blocked by SB203580 (P<0.01), but not by actinomycin D (P>0.05). It was also found subsequently that in the serum specimens collected from gonococci-infected but asymptomatic women, the progesterone level was higher than that in women with severe symptoms (P<0.01). Moreover, the yield of NO had an inverse correlation with progesterone. With these results it suggested that the rapid nongenomic effects of progesterone may inhibit iNOS activation and NO yields mediated by P38MAPK pathways, which were supposed to be concerned with asymptomatic women infected with gonococci.
Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. sunronarticleas@yahoo.cn
Rongyi
R
Chen
Yating
Y
Tu
Jiaxi
J
Lin
Weibing
W
She
Juan
J
Li
Zhihong
Z
Wu
Li
L
Xu
Hongxiang
H
Chen
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D009344:Neisseria gonorrhoeae / Q000254:growth & development* / Q000302:isolation & purification; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D009569:Nitric Oxide; D011374:Progesterone; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D048051:p38 Mitogen-Activated Protein Kinases