Transfection of nerve cells.
Transfection is a method of transforming cells based on the introduction into living cells of plasmids encoding a particular protein or RNA. This review describes the main methods of transfection and considers their advantages and disadvantages. Most attention is paid to lentivirus transduction as one of the most efficient methods for transforming nerve cells. The development of current transfection systems based on lentivirus vectors is described and a brief review of studies performed using in vivo and in vitro lentivirus transfection of nerve cells is presented.
Salozhin
S V
SV
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia. salodzin@yandex.ru
Bol'shakov
A P
AP
D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus; D009474:Neurons / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering