Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma.
Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79alphacy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gammadelta (TCR-gammadelta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin's lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs.
Rütgen
Barbara C
BC
Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria. barbara.ruetgen@vetmeduni.ac.at
Hammer
Sabine E
SE
Gerner
Wilhelm
W
Christian
Maria
M
de Arespacochaga
Abigail Guija
AG
Willmann
Michael
M
Kleiter
Miriam
M
Schwendenwein
Ilse
I
Saalmüller
Armin
A
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology*; D044963:Biopsy, Fine-Needle; D045744:Cell Line, Tumor / Q000737:chemistry / Q000473:pathology*; D002469:Cell Separation; D002999:Clone Cells / Q000737:chemistry / Q000473:pathology; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005434:Flow Cytometry; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta