Measurement of anterior chamber volume with rotating scheimpflug camera and anterior segment optical coherence tomography.
BACKGROUND
Measurement of anterior segment parameters plays an important role in diagnosis and treatment of glaucoma. The objective of this study was to evaluate the repeatability and reproducibility of anterior chamber volume (ACV) measurements with rotating scheimpflug camera (RSC) and to examine agreement with anterior segment optical coherence tomography (AS-OCT).


METHODS
Thirty nine healthy normal subjects were recruited from the Eye Center of Tongren Hospital. ACV was measured using RSC and AS-OCT in a randomly selected eye for each subject. For RSC measurements, both automatic and manual ACV measurements and 2 independent operators' ACV measurements were obtained. All subjects were invited for 3 visits within a week to evaluate repeatability and reproducibility of ACV measurement by RSC. Agreement was evaluated between RSC and AS-OCT.


RESULTS
Good repeatability and reproducibility were found for both automatic and manual ACV measurements obtained by RSC. For intrasession repeatability, coefficient of variation (CVw) and intraclass correlation coefficient (ICC) values for automatic were 3.52% and 0.98; the values for manual were 3.44% and 0.97, respectively. For intersession reproducibility, the respective CVw and ICC values were 3.96% and 0.96. Good agreement was also found in 2 operators for both automatic and manual ACV measurements; nevertheless, poor agreement was found between RSC and AS-OCT (95% confidence interval (CI) for agreement of automatic RSC measurement versus AS-OCT were -96.3 to 72.8 microl and 95% CI for agreement of manual RSC measurement versus AS-OCT were between -41.7 to 10.1 microl).


CONCLUSIONS
Both RSC automatic and manual ACV measurements showed good repeatability and reproducibility, and showed comparable agreement between 2 independent operators, but poor agreement was found between RSC and AS-OCT.
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Jing
J
Fu
Shu-ning
SN
Li
Xiao-zhen
XZ
Wang
Ge-wei
GW
Wu
Da-peng
DP
Mu
Jian
J
Wang
Ning-li
NL
Wang
D000328:Adult; D000867:Anterior Chamber / Q000033:anatomy & histology*; D000869:Anterior Eye Segment / Q000033:anatomy & histology*; D003315:Cornea / Q000033:anatomy & histology*; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation*; D005260:Female; D005901:Glaucoma / Q000175:diagnosis; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000379:methods*; D055815:Young Adult