Purification of MMPs and TIMPs.
A number of matrix metalloproteinases (MMPs) and their endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs) from natural sources have been identified. This chapter describes the purification methods of MMPs-1, -2, -3, -7, -8, -9, -10, -12, and -13 and TIMPs-1 and -2. The sources of the proteins and assay methods to detect their activities are also described.
Department of Physical Pharmacy, Meiji Pharmaceutical University, Tokyo, Japan.
Ken-Ichi
K
Shimokawa
Hideaki
H
Nagase
D000818:Animals; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification; D006801:Humans; D045726:Metalloproteases / Q000302:isolation & purification* / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D051381:Rats; D019714:Tissue Inhibitor of Metalloproteinases / Q000302:isolation & purification* / Q000378:metabolism
D004792:Enzyme Precursors; D019714:Tissue Inhibitor of Metalloproteinases; D045726:Metalloproteases