Determination of proteins induced in response to jasmonic acid and salicylic acid in resistant and susceptible cultivars of tomato.
Jasmonic acid (JA) and salicylic acid (SA) are signaling molecules that play key roles in the regulation of metabolic processes, reproduction, and defense against pathogens. The proteomics approach was used to identify proteins that are induced by JA and SA in the tomato cultivars Roma and Pant Bahr, which are susceptible and resistant to bacterial wilt, respectively. Threonine deaminase and leucine amino peptidase were upregulated, and ribulose-1,5-bisphosphate carboxylase/oxygenase small chain was downregulated by time-course application of JA. Translationally controlled tumor protein was upregulated by time-course application of SA. Protein disulfide isomerase was upregulated by application of either JA or SA. Proteins related to defense, energy, and protein destination/storage are suspected to be responsible for the susceptibility or resistance of the cultivars. Furthermore, in Roma, iron ABC transporter was upregulated by JA and down-regulated by SA. Iron ABC transporter plays a part in the signal transduction of both JA and SA in cultivars of tomato that are resistant to bacterial wilt.
National Institute of Crop Science, Tsukuba 305-8518, Japan.
Amber
A
Afroz
Muhammad Rashid
MR
Khan
Setsuko
S
Komatsu
D003517:Cyclopentanes / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D018551:Solanum lycopersicum / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000187:drug effects*; D020156:Salicylic Acid / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D020543:Proteome; C011006:jasmonic acid; D020156:Salicylic Acid