The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation.
Proteins with Pumilio RNA binding domains (Puf proteins) are ubiquitous in eukaryotes. Some Puf proteins bind to the 3'-untranslated regions of mRNAs, acting to repress translation and promote degradation; others are involved in ribosomal RNA maturation. The genome of Trypanosoma brucei encodes eleven Puf proteins whose function cannot be predicted by sequence analysis. We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing.
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
Dorothea
D
Droll
Stuart
S
Archer
Katelyn
K
Fenn
Praveen
P
Delhi
Keith
K
Matthews
Christine
C
Clayton
D002467:Cell Nucleus / Q000378:metabolism; D021983:Cyclophilins / Q000378:metabolism*; D048788:Growth and Development / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects / Q000235:genetics; D012335:RNA, Ribosomal / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D012335:RNA, Ribosomal; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D021983:Cyclophilins