A novel selenium and copper-containing peptide with both superoxide dismutase and glutathione peroxidase activities.
Superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS. In order to imitate the synergism of these enzymes, we designed and synthesized a novel 32-mer peptide (32P) on the basis of the previous 15-mer peptide with GPX activity and a 17-mer peptide with SOD activity. Upon the selenation and chelation of copper, the 32-mer peptide is converted to a new Se- and Cu-containing 32-mer peptide (Se-Cu-32P) and displays both SOD and GPX activities and its kinetics was studied. Moreover, the novel peptide was demonstrated to be able to better protect vero cells from the injury induced by xanthine oxidase (XOD)/xanthine/Fe2+ damage system than its parents. Thus, this bifunctional enzyme imitated the synergism of SOD and GPX and could be a better candidate of therapeutic medicine.
Zou
Xian-Feng
XF
Jilin University, Changchun, China.
Ji
Yue-Tong
YT
Gao
Gui
G
Zhu
Xue-Jun
XJ
Lv
Shao-Wu
SW
Yan
Fei
F
Han
Si-Ping
SP
Chen
Xing
X
Gao
Chang-Cheng
CC
Liu
Junqiu
J
Luo
Gui-Min
GM
D000818:Animals; D002522:Chlorocebus aethiops; D003300:Copper / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D018384:Oxidative Stress / Q000187:drug effects; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012643:Selenium / Q000737:chemistry; D013482:Superoxide Dismutase / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D014709:Vero Cells
D010455:Peptides; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium