Virulence analysis and gene expression profiling of the pigment-deficient mutant of Xanthomonas oryzae pathovar oryzae.
Xanthomonas oryzae pathovar oryzae (Xoo) causes bacterial blight disease in rice (Oryza sativa L.). For a study of function, we constructed a random insertion mutant library of Xoo using a Tn5 transposon and isolated the mutant strain (M11; aroK::Tn5) that had extremely low pigment production. In addition, M11 had decreased virulence against the susceptible rice cultivar IR24. Thermal asymmetric interlaced-PCR and sequence analysis of M11 revealed that the transposon was inserted into the aroK gene (which encodes a shikimate kinase). To investigate the expression patterns of the pigment- and virulence-deficient mutant, DNA microarray analysis was performed. In addition, reverse transcriptase-PCR was performed to confirm the expression levels of several genes, including the aro genes of the aroK mutant. Our findings reveal that several crucial genes for virulence, including cellulase and hypersensitive response and pathogenicity (hrp) genes, were regulated by mutations in the aroK gene.
Park
Young-Jin
YJ
National Academy of Agricultural Science, Rural Development Administration, Suwon, Korea.
Song
Eun-Sung
ES
Noh
Tae-Hwan
TH
Kim
Hyungtae
H
Yang
Kap-Seok
KS
Hahn
Jang-Ho
JH
Kang
Hee-Wan
HW
Lee
Byoung-Moo
BM
D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000382:microbiology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010860:Pigments, Biological / Q000096:biosynthesis; D010935:Plant Diseases / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis; D014974:Xanthomonas / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010860:Pigments, Biological; D037521:Virulence Factors; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase