Comparison of two different isolation methods of benzimidazoles and their metabolites in the bovine liver by solid-phase extraction and liquid chromatography-diode array detection.
A new analytical method that uses high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the analysis of 14 benzimidazoles residues, including metabolites, in bovine liver. Samples were extracted using two different extraction procedures: with phosphate buffer after enzymatic hydrolysis (method A) or using organic solvent, i.e. acetonitrile (method B). Then, samples were purified on a strong cation exchange (SCX) cartridge and analyzed in HPLC/DAD. The recovery percentages, obtained spiking the matrix (liver) at concentrations of 500 and 100 microg kg(-1) with a standard mixture of benzimidazoles, were in the range 6-101% and 80-102% for methods A and B, respectively. The repeatability of the methods was assessed in all cases by the % of correlation value (CV) that was lower than 19%. The limits of quantification (LOQs) in the matrix for methods A and B were in the range 40-60 and 20-50 microg kg(-1), respectively. The best of the two methods, method B, was used for the analysis of 10 bovine liver samples.
Dipartimento di Scienze Chimiche, Facoltà di Farmacia, Università di Camerino, via S. Agostino 1, Camerino, Italy.
Giovanni
G
Caprioli
Gloria
G
Cristalli
Roberta
R
Galarini
Dania
D
Giacobbe
Massimo
M
Ricciutelli
Sauro
S
Vittori
Yanting
Y
Zuo
Gianni
G
Sagratini
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001562:Benzimidazoles / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
D000097:Acetonitriles; D001562:Benzimidazoles; D010710:Phosphates; D010447:Peptide Hydrolases; C032159:acetonitrile