Polyelectrolyte stabilized drug nanoparticles via flash nanoprecipitation: a model study with beta-carotene.
Polyelectrolyte protected beta-carotene nanoparticles (nanosuspensions) with average diameter of <100 nm were achieved by turbulent mixing and flash nanoprecipitation (FNP). Three types of multi-amine functional polyelectrolytes, epsilon-polylysine (epsilon-PL), poly(ethylene imine) (PEI), and chitosan, were investigated to electrosterically protect the nanoparticles. Particle size and distribution were measured by dynamic light scattering (DLS); particles were imaged via scanning electron microscopy (SEM) and cryogenic transmission electron microscopy (cryo-TEM). Low pH and high polyelectrolyte molecular weight gave the smallest and most stable particles. High drug loading capacity, >80 wt%, was achieved by using either PEI or chitosan. X-ray diffraction (XRD) patterns showed that beta-carotene nanoparticles were amorphous. These findings open the way for utilization of FNP for preparation of nanoparticles with enhanced bioavailability for highly water insoluble drugs.
Zhu
Zhengxi
Z
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Margulis-Goshen
Katrin
K
Magdassi
Shlomo
S
Talmon
Yeshayahu
Y
Macosko
Christopher W
CW
D001682:Biological Availability; D004573:Electrolytes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D053758:Nanoparticles; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D014961:X-Ray Diffraction; D019207:beta Carotene / Q000737:chemistry* / Q000493:pharmacokinetics
D004573:Electrolytes; D004364:Pharmaceutical Preparations; D019207:beta Carotene