Analysis of lidocaine interactions with serum proteins using high-performance affinity chromatography.
High-performance affinity chromatography was used to study binding by the drug lidocaine to human serum albumin (HSA) and alpha(1)-acid glycoprotein (AGP). AGP had strong binding to lidocaine, with an association equilibrium constant (K(a)) of 1.1-1.7 x 10(5) M(-1) at 37 degrees C and pH 7.4. Lidocaine had weak to moderate binding to HSA, with a K(a) in the range of 10(3) to 10(4) M(-1). Competitive experiments with site selective probes showed that lidocaine was interacting with Sudlow site II of HSA and the propranolol site of AGP. These results agree with previous observations in the literature and provide a better quantitative understanding of how lidocaine binds to these serum proteins and is transported in the circulation. This study also demonstrates how HPAC can be used to examine the binding of a drug with multiple serum proteins and provide detailed information on the interaction sites and equilibrium constants that are involved in such processes.
Soman
Sony
S
Chemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE 68588-0304, USA.
Yoo
Michelle J
MJ
Jang
Yoon Jeong
YJ
Hage
David S
DS
D001667:Binding, Competitive; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008012:Lidocaine / Q000737:chemistry / Q000378:metabolism*; D016014:Linear Models; D009961:Orosomucoid / Q000737:chemistry / Q000378:metabolism*; D011433:Propranolol / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
D009961:Orosomucoid; D012709:Serum Albumin; D008012:Lidocaine; D011433:Propranolol