In situ hybridization of Helobdella (leech) embryos.
The purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.
Weisblat
David A
DA
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA. weisblat@berkeley.edu
Kuo
Dian-Han
DH
D000345:Affinity Labels / Q000737:chemistry; D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000196:embryology*; D005821:Genetic Techniques; D017403:In Situ Hybridization / Q000379:methods*; D007865:Leeches / Q000196:embryology* / Q000502:physiology*; D015345:Oligonucleotide Probes / Q000378:metabolism; D010539:Permeability; D013997:Time Factors; D047048:Vitellins / Q000737:chemistry
D000345:Affinity Labels; D015345:Oligonucleotide Probes; D047048:Vitellins