Australian dingoes are definitive hosts of Neospora caninum.
To provide objective data on the potential role of dingoes (Canis lupus dingo) in the life cycle of Neospora caninum in Australia, the production of N. caninum oocysts by experimentally infected canids was investigated. Three dingo pups raised in captivity and three domestic dogs were fed tissue from calves infected with an Australian isolate of N. caninum, Nc-Nowra. Oocysts of N. caninum, confirmed by species-specific PCR, were shed in low numbers by one dingo pup at 12-14 days p.i. The remaining animals did not shed oocysts. Furthermore, the blood from two out of three dingoes tested positive for DNA of N. caninum using PCR tests at 14 and 28 days p.i. Oocyst shedding from the intestinal tract of a dingo demonstrates that dingoes are definitive hosts of N. caninum and horizontal transmission of N. caninum from dingoes to farm animals and wildlife may occur in Australia.
King
Jessica S
JS
Faculty of Veterinary Science, University of Sydney, NSW, Australia. jessica.king@sydney.edu.au
Slapeta
Jan
J
Jenkins
David J
DJ
Al-Qassab
Sarwat E
SE
Ellis
John T
JT
Windsor
Peter A
PA
D000818:Animals; D000831:Animals, Newborn; D001315:Australia; D002417:Cattle; D016054:DNA, Protozoan / Q000097:blood / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D008018:Life Cycle Stages; D008297:Male; D008969:Molecular Sequence Data; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification*; D033661:Oocysts; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019596:Wolves / Q000469:parasitology*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal