Qualitative and quantitative analysis of anthraquinone derivatives in rhizomes of tissue culture-raised Rheum emodi Wall. plants.
This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 microM 6-benzylaminopurine (BAP) and 5.0 microM indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months.
Malik
Sonia
S
Institute of Himalayan Bioresource Technology, Palampur, HP, India.
Sharma
Nandini
N
Sharma
Upendra K
UK
Singh
Narendra P
NP
Bhushan
Shashi
S
Sharma
Madhu
M
Sinha
Arun K
AK
Ahuja
Paramvir S
PS
D000880:Anthraquinones / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012250:Rheum / Q000737:chemistry* / Q000254:growth & development; D027343:Rhizome / Q000737:chemistry*
D000880:Anthraquinones; D006027:Glycosides; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid