Non-invasive diagnosis of liver diseases by breath analysis using an optimized ion-molecule reaction-mass spectrometry approach: a pilot study.
Breath composition is altered in liver diseases. We tested if ion-molecule-reaction mass spectrometry (IMR-MS) combined with a new statistical modality improves the diagnostic accuracy of breath analysis in liver diseases. We analysed 114 molecules in the breath of 126 individuals (healthy controls, and patients with non-alcoholic and alcoholic fatty liver disease and liver cirrhosis) by IMR-MS. Characteristic exhalation patterns were identified for each group. Combining two to seven molecules in the new stacked feature ranking model reached a diagnostic accuracy (area under the curve) for individual liver diseases between 0.88 and 0.97. IMR-MS followed by sophisticated statistical analysis is a promising tool for liver diagnostics by breath analysis.
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Innsbruck, Austria. gunda.millonig@uni-heidelberg.de
Gunda
G
Millonig
Siegfried
S
Praun
Michael
M
Netzer
Christian
C
Baumgartner
Albert
A
Dornauer
Sebastian
S
Mueller
Johannes
J
Villinger
Wolfgang
W
Vogel
D000079:Acetaldehyde / Q000032:analysis; D000328:Adult; D000368:Aged; D015415:Biomarkers; D001944:Breath Tests; D002070:Butadienes / Q000032:analysis; D000431:Ethanol / Q000032:analysis; D005234:Fatty Liver / Q000175:diagnosis; D005235:Fatty Liver, Alcoholic / Q000175:diagnosis; D005260:Female; D045782:Hemiterpenes / Q000032:analysis; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis; D008107:Liver Diseases / Q000145:classification / Q000175:diagnosis*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010420:Pentanes / Q000032:analysis; D010865:Pilot Projects
D015415:Biomarkers; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D000431:Ethanol; D000079:Acetaldehyde