Genomic analyses of hormone signaling and gene regulation.
Many cellular signaling pathways ultimately control specific patterns of gene expression in the nucleus through a variety of signal-regulated transcription factors (TFs), including nuclear hormone receptors (NRs). The advent of genomic technologies for examining signal-regulated transcriptional responses and TF binding on a genomic scale has dramatically increased our understanding of the cellular programs that control hormonal signaling and gene regulation. Studies of TFs, especially NRs, using genomic approaches have revealed novel and unexpected features of hormone-regulated transcription, and a global view is beginning to emerge. In this review, we discuss the genomic methodologies that have been applied to the study of hormone-regulated gene expression, the results that have been obtained from using them, and the future prospects for these approaches. Given the wealth of information about hormone-dependent gene regulation by NRs, we have focused this review on the knowledge gained from genomic studies of their function.
Cheung
Edwin
E
Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore. cheungcwe@gis.a-star.edu.sg
Kraus
W Lee
WL
D000818:Animals; D002843:Chromatin / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D023281:Genomics; D006728:Hormones / Q000235:genetics* / Q000502:physiology; D006801:Humans; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors
D002843:Chromatin; D006728:Hormones; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors