Real-time fluorescent quantitative polymerase chain reaction study of polo-like kinase-1 in pterygia.
This study was designed to examine the expression of polo-like kinase 1 (PLK1) mRNA in 16 pterygia and 13 normal conjunctival tissue specimens using real-time fluorescent quantitative polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the housekeeping gene. The difference in threshold cycle value (DeltaC(t)) was derived for PLK1 and GAPDH for each sample assayed, and the difference between the paired samples (DeltaDeltaC(t)) was calculated. The mean +/- SD DeltaC(t) of PLK1 mRNA was 9.56 +/- 1.30 in pterygia compared with 10.71 +/- 1.39 in normal conjunctiva. The expression of PLK1 mRNA in pterygium was 2.08 - 2.36 times that in normal conjunctiva; this difference was statistically significant. Real-time fluorescent quantitative PCR analysis appears to be effective and sensitive when determining the level of PLK1 mRNA expression. Using this method, it was demonstrated that PLK1 mRNA is over-expressed in pterygia, indicating a probable role for PLK1 in their development.
Lu
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Department of Ophthalmology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Lou
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DH
Zhu
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D000328:Adult; D000368:Aged; D052160:Benzothiazoles; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D003959:Diamines; D005260:Female; D005453:Fluorescence; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D011625:Pterygium / Q000201:enzymology* / Q000235:genetics*; D011804:Quinolines; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D000097869:Polo-Like Kinase 1
D052160:Benzothiazoles; D018797:Cell Cycle Proteins; D003959:Diamines; D009930:Organic Chemicals; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D011804:Quinolines; D012333:RNA, Messenger; D000097869:Polo-Like Kinase 1; C098022:SYBR Green I