Assay of matrix metalloproteinases against matrix substrates.
The assays described allow the activity of members of the matrix metalloproteinase (MMP) family that degrade collagen, gelatin and casein substrates to be measured. The protocols described include the preparation of radiolabeled substrates, methods for the separation of degraded product from undegraded substrate, and methods for the activation of MMPs. The advantages and disadvantages of these methods are discussed in relation to immunoassays that measure the amount of individual MMPs.
Musculoskeletal Research Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Tim E
TE
Cawston
Rachel L
RL
Lakey
Andrew D
AD
Rowan
D000818:Animals; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D057075:Enzyme Assays / Q000379:methods*; D004792:Enzyme Precursors / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D013194:Staining and Labeling; D013379:Substrate Specificity / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; C010024:4-aminophenylmercuriacetate; D014357:Trypsin; D020782:Matrix Metalloproteinases; D010662:Phenylmercuric Acetate