Hydrogen-rich saline ameliorates the severity of l-arginine-induced acute pancreatitis in rats.
Molecular hydrogen, which reacts with the hydroxyl radical, has been considered as a novel antioxidant. Here, we evaluated the protective effects of hydrogen-rich saline on the l-arginine (l-Arg)-induced acute pancreatitis (AP). AP was induced in Sprague-Dawley rats by giving two intraperitoneal injections of l-Arg, each at concentrations of 250mg/100g body weight, with an interval of 1h. Hydrogen-rich saline (>0.6mM, 6ml/kg) or saline (6ml/kg) was administered, respectively, via tail vein 15min after each l-Arg administration. Severity of AP was assessed by analysis of serum amylase activity, pancreatic water content and histology. Samples of pancreas were taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa B (NF-kappaB) were detected with immunohistochemistry. Hydrogen-rich saline treatment significantly attenuated the severity of l-Arg-induced AP by ameliorating the increased serum amylase activity, inhibiting neutrophil infiltration, lipid oxidation and pancreatic tissue edema. Moreover, hydrogen-rich saline treatment could promote acinar cell proliferation, inhibit apoptosis and NF-kappaB activation. These results indicate that hydrogen treatment has a protective effect against AP, and the effect is possibly due to its ability to inhibit oxidative stress, apoptosis, NF-kappaB activation and to promote acinar cell proliferation.
Department of General Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, PR China.
Han
H
Chen
Yan Ping
YP
Sun
Yang
Y
Li
Wen Wu
WW
Liu
Hong Gang
HG
Xiang
Lie Ying
LY
Fan
Qiang
Q
Sun
Xin Yun
XY
Xu
Jian Mei
JM
Cai
Can Ping
CP
Ruan
Ning
N
Su
Rong Lin
RL
Yan
Xue Jun
XJ
Sun
Qiang
Q
Wang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001120:Arginine / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D006859:Hydrogen / Q000627:therapeutic use*; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use
D012965:Sodium Chloride; D006859:Hydrogen; D001120:Arginine