Response of Pseudomonas putida KT2440 to phenol at the level of membrane proteome.
This study led to the extension and refinement of our current model for the global response of Pseudomonas putida KT2440 to phenol by getting insights into the adaptive response mechanisms involving the membrane proteome. A two-dimensional gel electrophoresis based protocol was optimized to allow the quantitative comparison of membrane proteins, by combining inner and outer membrane fractionation with membrane protein solubilization using the detergent dodecylmaltoside. Following phenol exposure, a coordinate increased content of protein subunits of known or putative solvent efflux pump systems (e.g. TtgA, TtgC, Ttg2A, Ttg2C, and PP_1516-7) and a decreased content of porins OprB, OprF, OprG and OprQ was registered, consistent with an adaptive response to reduce phenol intracellular concentration. This adaptive response may in part be mediated by post-translational modifications, as suggested by the relative content of the multiple forms identified for a few porins and efflux pump subunits. Results also suggest the important role of protein chaperones, of cell envelope and cell surface and of a more active respiratory chain in the response to phenol. All these mechanistic insights may be extended to Pseudomonas adaptation to solvents, of possible impact in biodegradation, bioremediation and biocatalysis.
IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Universidade Técnica de Lisboa, Lisboa, Portugal.
Catarina
C
Roma-Rodrigues
Pedro M
PM
Santos
Dirk
D
Benndorf
Erdmann
E
Rapp
Isabel
I
Sá-Correia
D001426:Bacterial Proteins / Q000032:analysis / Q000187:drug effects*; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D019800:Phenol / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D016958:Pseudomonas putida / Q000187:drug effects* / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome; D019800:Phenol