A bicistronic lentiviral vector based on the 1D/2A sequence of foot-and-mouth disease virus expresses proteins stoichiometrically.
Classic IRES sequences are notorious for exerting biased expression in favor of upstream coding regions when placed into polycistronic vectors. Here, we report the development of a bicistronic lentiviral system based on the 1D/2A sequence from the foot-and-mouth disease virus that is able to maintain tightly balanced control of upstream and downstream protein expression for several days at a stoichiometry very closely approaching 1.0. Our results suggest that the 1D/2A sequence can be optimized in an FUGW lentiviral setting to coordinate expression of multiple polypeptides, presenting a potentially valuable tool to signaling network researchers and to the gene therapy community.
Torres
Viviana
V
Department of Medical Technology, Universidad de Antofagasta, Avenida Angamos 601, Codigo Postal 1270300, Antofagasta, Chile.
Barra
Lucia
L
Garcés
Felipe
F
Ordenes
Kely
K
Leal-Ortiz
Sergio
S
Garner
Craig C
CC
Fernandez
Fabian
F
Zamorano
Pedro
P
D036022:Capsid Proteins / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D014764:Viral Proteins; C057421:virus protein 2A