Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 degrees C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.
Tsukiyama
Takuji
T
Laboratory of Plant Breeding, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Japan.
Lee
Jongwon
J
Okumoto
Yutaka
Y
Teraishi
Masayoshi
M
Tanisaka
Takatoshi
T
Inouye
Kuniyo
K
D003001:Cloning, Molecular; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D010940:Plant Proteins; D025801:Ubiquitin