Slow non-specific accumulation of 2'-deoxy and 2'-O-methyl oligonucleotide probes at mitochondria in live cells.
Molecular beacons (MBs) have the potential to provide a powerful tool for rapid RNA detection in living cells, as well as monitoring the dynamics of RNA expression in response to external stimuli. To exploit this potential, it is necessary to distinguish true signal from background signal due to non-specific interactions. Here, we show that, when cyanine-dye labeled 2'-deoxy and 2'-O-methyl oligonucleotide probes are inside living cells for >5 h, most of their signals co-localize with mitochondrial staining. These probes include random-sequence MB, dye-labeled single-strand linear oligonucleotide and dye-labeled double-stranded oligonucleotide. Using carbonyl cyanide m-chlorophenyl hydrazone treatment, we found that the non-specific accumulation of oligonucleotide probes at mitochondria was driven by mitochondrial membrane potential. We further demonstrated that the dye-labeled oligonucleotide probes were likely on/near the surface of mitochondria but not inside mitochondrial inner membrane. Interestingly, oligonucleotides probes labeled respectively with Alexa Fluor 488 and Alexa Fluor 546 did not accumulate at mitochondria, suggesting that the non-specific interaction between dye-labeled ODN probes and mitochondria is dye-specific. These results may help design and optimize fluorescence imaging probes for long-time RNA detection and monitoring in living cells.
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Won Jong
WJ
Rhee
Gang
G
Bao
D002232:Carbocyanines / Q000737:chemistry; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000737:chemistry*; D051336:Mitochondrial Membranes / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000032:analysis* / Q000737:chemistry
D002232:Carbocyanines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C089187:cyanine dye 3