The CXCL12/CXCR4 pair in aged human thymus.
CXCL12 is an important CXC chemokine involved in numerous biological processes. We had previously demonstrated the synergistic participation of CXCL12 and IL-7 in the control of both survival and proliferation of CD34(+) human thymic lymphoid progenitors. On this basis, we hypothesize a presumptive role for CXCL12 and its receptor, CXCR4, in the thymus involution. In this respect, in the current report we describe the expression of both molecules in the human thymus during aging. Our results demonstrate that, despite the profound alterations observed in the thymic epithelial microenvironment of aged thymuses, the proportions of different CD4/CD8 thymocyte subsets do not undergo significant variations. Remarkably, a strong CXCL12 expression was found in older thymuses, which appeared in the same locations as in younger thymuses: the subcapsulary and medullary areas. The proportions of CXCR4(+) cells, most of them belonging to the CD3(-) compartment, showed no important variations in the older thymuses. However, within the CD34(+) cell population, a significant reduction in the expression of CXCR4 molecules was observed.
Center for Cytometry and Microscopy, Complutense University of Madrid, Madrid, Spain. carmenh@bio.ucm.es
Carmen
C
Hernández-López
Alberto
A
Varas
Rosa
R
Sacedón
Victor G
VG
Martínez
Laura
L
Hidalgo
Jaris
J
Valencia
Agustín G
AG
Zapata
Angeles
A
Vicente
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology*; D018952:Antigens, CD34 / Q000378:metabolism; D001284:Atrophy / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D054377:Chemokine CXCL12 / Q000378:metabolism*; D006706:Homeostasis / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D019718:Receptors, CXCR4 / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D055815:Young Adult
D018952:Antigens, CD34; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4