The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins.
Tuberculosis remains a major human health problem worldwide, and strategies for its prevention include the generation and characterization of new recombinant vaccines containing immunodominant antigens from Mycobacterium tuberculosis. By comparing the secretomes of wild-type Mycobacterium bovis and a PstS1-recombinant M. bovis BCG vaccine substrain (rBCG38), we identified six conserved hypothetical proteins (BCG2696, BCG1674, BCG0372, BCG0427, BCG2436c, and BCG3053) that are differentially expressed. Our findings will aid in the identification of highly immunogenic proteins present in rBCG.
Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio de Investigación, 4 degrees piso, Av. Universidad #3000, Coyoacán, 04510 México, D.F., Mexico.
Mauricio
M
Rodríguez-Alvarez
Iliana D
ID
Palomec-Nava
Guillermo
G
Mendoza-Hernández
Yolanda
Y
López-Vidal
D001500:BCG Vaccine / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D007525:Isoelectric Focusing; D009163:Mycobacterium bovis / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000276:immunology
D001500:BCG Vaccine; D001426:Bacterial Proteins; D016056:Immunodominant Epitopes; D014614:Vaccines, Synthetic