Expression of mutant RPA in human cancer cells causes telomere shortening.
Replication protein A (RPA) binds to single-stranded DNA generated during DNA replication and other processes. The roles of RPA in telomere maintenance have been demonstrated in yeasts, but not in telomerase-positive human cells. In this study, we found that expression of mutant RPA70 in human cells caused telomere shortening, suggesting that RPA is required for telomere-length regulation in human cancer cells.
Kobayashi
Yuka
Y
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
Sato
Koichiro
K
Kibe
Tatsuya
T
Seimiya
Hiroyuki
H
Nakamura
Asako
A
Yukawa
Masashi
M
Tsuchiya
Eiko
E
Ueno
Masaru
M
D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D051716:Replication Protein A / Q000235:genetics* / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*
D051716:Replication Protein A