A transgenic Cre mouse line for the study of cortical and hippocampal development.
Wnt signaling regulates cortical and hippocampal development. In a previous study we found that a particular Wnt receptor, Frizzled9 (Fzd9), was selectively expressed in both the developing and adult hippocampus. Taking advantage of the specificity of this promoter, we generated a transgenic cre mouse line using the putative control elements of the Fzd9 gene. In the Fzd9-cre mice, Cre is mainly detected in the developing cortex and hippocampus and is confined to the CA fields and dentate gyrus in adults. Furthermore, by crossing the Fzd9-cre mouse with the ROSA26 reporter line, we examined the activity of Cre and found that it has very high recombination efficiency. Thus, this mouse line will likely prove to be a useful tool for studying cortical and hippocampal development via activation or inactivation of interesting genes.
Zhou
Wei
W
Key Laboratory of Developmental Genes and Human Diseases, MOE, Institute of Life Science, Southeast University, Nanjing, Jiangsu, People's Republic of China.
Zhang
Ying
Y
Li
Yiping
Y
Wei
Yu-Sheng
YS
Liu
Guang
G
Liu
De-Pei
DP
Pleasure
Samuel J
SJ
Xie
Wei
W
Zhao
Chunjie
C
D000818:Animals; D002540:Cerebral Cortex / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology* / Q000378:metabolism; D051157:Frizzled Receptors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000196:embryology* / Q000378:metabolism; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated; D017981:Receptors, Neurotransmitter / Q000235:genetics; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D051157:Frizzled Receptors; C403212:Fzd9 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D011506:Proteins; D022661:RNA, Untranslated; D017981:Receptors, Neurotransmitter; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase