Wine yeast molecular typing using a simplified method for simultaneously extracting mtDNA, nuclear DNA and virus dsRNA.
Quick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains. We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA. It is simpler, cheaper, and faster than the previously reported methods. It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different methods for the separate extraction of each nucleic acid type. The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing.
Departamento de Ciencias Biomédicas (Area de Microbiología), Facultad de Ciencias (Antiguo Rectorado), Universidad de Extremadura, 06071 Badajoz, Spain.
Matilde
M
Maqueda
Emiliano
E
Zamora
Nieves
N
Rodríguez-Cousiño
Manuel
M
Ramírez
D002467:Cell Nucleus / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000821:virology
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012330:RNA, Double-Stranded; D012367:RNA, Viral