KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1.
In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling. [BMB reports 2010; 43(1): 17-22].
The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, Peoples' Republic of China.
Junjian
J
Hu
Wuzhou
W
Yuan
Ming
M
Tang
Yuequn
Y
Wang
Xiongwei
X
Fan
Xiaoyang
X
Mo
Yongqing
Y
Li
Zaochu
Z
Ying
Yongqi
Y
Wan
Karen
K
Ocorr
Rolf
R
Bodmer
Yun
Y
Deng
Xiushan
X
Wu
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D027602:Serum Response Element; D015398:Signal Transduction; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D047908:Intracellular Signaling Peptides and Proteins; C549143:KBTBD7 protein, human; D034741:RNA, Small Interfering; D015534:Trans-Activators; D018808:Transcription Factor AP-1; D020928:Mitogen-Activated Protein Kinases