Larkinella bovis sp. nov., isolated from fermented bovine products, and emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006.
A novel bacterial strain, designated M2T2B15(T), was isolated from fermented bovine products and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Cells were strictly aerobic, Gram-reaction-negative, oxidase- and catalase-positive rods that lacked flagella and were motile by gliding. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that strain M2T2B15(T) was related most closely to Larkinella insperata LMG 22510(T) (94.4 % similarity) but shared <87 % similarity with other members of the phylum Bacteroidetes. The major cellular fatty acids were C(16 : 1)ω5c, iso-C(15 : 0) and iso-C(17 : 0) 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylserine, two unidentified aminophospholipids and two unidentified polar lipids. Menaquinone 7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain M2T2B15(T) was 52&emsp14;mol%. The phenotypic, genotypic and phylogenetic data presented clearly indicate that strain M2T2B15(T) represents a novel species of the genus Larkinella, for which the name Larkinella bovis sp. nov. is proposed. The type strain is M2T2B15(T) (=KACC 14040(T) =NBRC 106324(T)). Emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006 are also proposed.
Anandham
Rangasamy
R
Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625 104, Tamil Nadu, India.|Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Weon
Hang-Yeon
HY
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kim
Soo-Jin
SJ
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kim
Yi-Seul
YS
Korean Agricultural Culture Collection (KACC), National Agro Biodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Gandhi
Pandiyan Indira
PI
Regional Research Station, Tamil Nadu Agricultural University, Vriddhachalam 606001, Tamil Nadu, India.
Kim
Yong Ki
YK
Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Jee
Hyeong Jin
HJ
Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
D000818:Animals; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D016000:Cluster Analysis; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D008124:Locomotion; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011090:Polyenes / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011090:Polyenes; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C017313:flexirubins; D010088:Oxidoreductases; D002374:Catalase