Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent Trichoderma asperellum T203.
1-aminocyclopropane-1-carboxylate (ACC) deaminase activity was evaluated in the biocontrol and plant growth-promoting fungus Trichoderma asperellum T203. Fungal cultures grown with ACC as the sole nitrogen source showed high enzymatic activity. The enzyme encoding gene (Tas-acdS) was isolated, and an average 3.5-fold induction of the gene by 3 mM ACC was detected by real-time PCR. Escherichia coli bacteria carrying the intron-free cDNA of Tas-acdS cloned into the vector pAlter-EX1 under the control of the tac promoter revealed specific ACC deaminase (ACCD) activity and the ability to promote canola (Brassica napus) root elongation in pouch assays. RNAi silencing of the ACCD gene in T. asperellum showed decreased ability of the mutants to promote root elongation of canola seedlings. These data suggest a role for ACCD in the plant root growth-promotion effect by T. asperellum.
Viterbo
Ada
A
Department of Plant Pathology and Microbiology, The Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel. viterbo@agri.huji.ac.il
Landau
Udi
U
Kim
Sofia
S
Chernin
Leonid
L
Chet
Ilan
I
D000595:Amino Acid Sequence; D000598:Amino Acids, Cyclic / Q000378:metabolism*; D029688:Brassica napus / Q000254:growth & development* / Q000382:microbiology; D019755:Carbon-Carbon Lyases / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D020868:Gene Silencing; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development
D000598:Amino Acids, Cyclic; D004271:DNA, Fungal; D005656:Fungal Proteins; C023863:1-aminocyclopropane-1-carboxylic acid; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases; D009584:Nitrogen