Molecular cloning of the first human monoclonal antibodies neutralizing with high potency swine-origin influenza A pandemic virus (S-OIV).
The pandemic caused by the new H1N1 swine-origin influenza virus (S-OIV) strain is a worldwide health emergency and alternative therapeutic and prophylactic options are greatly needed. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza strain at very low concentrations were cloned from a patient who had a broad-range anti-H1N1 serum neutralizing activity. The two HMabs neutralized S-OIV with an IC50 of 2.8 and 4 microg/mL. The genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered with the potentially of representing a novel drug to be used in the prophylaxis and the treatment of this human infection. This is the first report of molecular cloning of human monoclonal antibodies against the new pandemic swine-origin influenza virus.
Burioni
Roberto
R
Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milano, Italy. burioni.roberto@hsr.it
Canducci
Filippo
F
Mancini
Nicasio
N
Clementi
Nicola
N
Sassi
Monica
M
De Marco
Donata
D
Saita
Diego
D
Diotti
Roberta Antonia
RA
Sautto
Giuseppe
G
Sampaolo
Michela
M
Clementi
Massimo
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D057134:Antibodies, Neutralizing / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D004196:Disease Outbreaks; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D008875:Middle Aged; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G