SRD1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweetpotato (Ipomoea batatas).
A sweetpotato (Ipomoea batatas cv. 'Jinhongmi') MADS-box protein cDNA (SRD1) has been isolated from an early stage storage root cDNA library. The role of the SRD1 gene in the formation of the storage root in sweetpotato was investigated by an expression pattern analysis and characterization of SRD1-overexpressing (ox) transgenic sweetpotato plants. Transcripts of SRD1 were detected only in root tissues, with the fibrous root having low levels of the transcript and the young storage root showing relatively higher transcript levels. SRD1 mRNA was mainly found in the actively dividing cells, including the vascular and cambium cells of the young storage root. The transcript level of SRD1 in the fibrous roots increased in response to 1000 muM indole-3-acetic acid (IAA) applied exogenously. During the early stage of storage root development, the endogenous IAA content and SRD1 transcript level increased concomitantly, suggesting an involvement of SRD1 during the early stage of the auxin-dependent development of the storage root. SRD1-ox sweetpotato plants cultured in vitro produced thicker and shorter fibrous roots than wild-type plants. The metaxylem and cambium cells of the fibrous roots of SRD1-ox plants showed markedly enhanced proliferation, resulting in the fibrous roots of these plants showing an earlier thickening growth than those of wild-type plants. Taken together, these results demonstrate that SRD1 plays a role in the formation of storage roots by activating the proliferation of cambium and metaxylem cells to induce the initial thickening growth of storage roots in an auxin-dependent manner.
Noh
Seol Ah
SA
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Lee
Haeng-Soon
HS
Huh
Eun Joo
EJ
Huh
Gyung Hye
GH
Paek
Kyung-Hee
KH
Shin
Jeong Sheop
JS
Bae
Jung Myung
JM
D049109:Cell Proliferation / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000494:pharmacology*; D027723:Ipomoea batatas / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007210:Indoleacetic Acids; D010940:Plant Proteins