Quantification and improvement of error rate during ligase detection reaction.
We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3' ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3' ends.
Ginya
Harumi
H
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, Japan. harumi.ginya@pss.co.jp
Matsushita
Ryouhei
R
Yohda
Masafumi
M
D020137:Base Pair Mismatch / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D004795:Enzyme Stability; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008962:Models, Theoretical; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity
D008025:Ligases