Topical anti-inflammatory potential of Physalin E from Physalis angulata on experimental dermatitis in mice.
The anti-inflammatory effect of physalin E, a seco-steroid isolated from Physalis angulata L. was evaluated on acute and chronic models of dermatitis induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and oxazolone, respectively, in mouse ear. The changes in ear edema/thickness, production of pro-inflammatory cytokines (TNF-alpha and IFN-gamma), myeloperoxidase (MPO) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. Similar to dexamethasone, topically applied Physalin E (0.125; 0.25 and 0.5 mg/ear) potently inhibited the TPA and oxazolone-induced dermatitis, leading to substantial reductions in ear edema/thickness, pro-inflammatory cytokines, and MPO activity. These effects were reversed by mifepristone, a steroid antagonist and confirmed by immunohistochemical and histopathological analysis. The data suggest that physalin E may be a potent and topically effective anti-inflammatory agent useful to treat the acute and chronic skin inflammatory conditions.
Pinto
N B
NB
Department of Physiology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil.
Morais
T C
TC
Carvalho
K M B
KM
Silva
C R
CR
Andrade
G M
GM
Brito
G A C
GA
Veras
M L
ML
Pessoa
O D L
OD
Rao
V S
VS
Santos
F A
FA
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D003877:Dermatitis, Contact / Q000188:drug therapy*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010081:Oxazolone / Q000633:toxicity; D032321:Physalis / Q000737:chemistry*; D012632:Secosteroids / Q000627:therapeutic use*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D000893:Anti-Inflammatory Agents; D012632:Secosteroids; C553897:physalin E; D010081:Oxazolone; D013755:Tetradecanoylphorbol Acetate