Molecular analysis of antimicrobial resistance in gram-negative bacteria isolated from fish farms in Egypt.
As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range of antimicrobial resistance genes in Gram-negative bacteria isolated from water samples taken from fish farms in the northern part of Egypt. Ninety-one out of two hundred seventy-four (33.2%) non-repetitive isolates of Gram-negative bacteria showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing results showed that 72 (26.3%) isolates contain tetracycline resistance genes and 19 (6.9%) isolates were positive for class 1 integrons with 12 different gene cassettes. The beta-lactamase-encoding genes were identified in 14 (5.1%) isolates. The plasmid-mediated quinolone resistance genes, qnr and aac(6')-Ib-cr, were identified in 16 (5.8%) and 3 (1.1%) isolates, respectively. Finally, the florphenicol resistance gene, floR, was identified in four (1.5%) isolates. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from fish farms in Africa.
Ishida
Yojiro
Y
Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Japan.
Ahmed
Ashraf M
AM
Mahfouz
Nadia B
NB
Kimura
Tomomi
T
El-Khodery
Sabry A
SA
Moawad
Amgad A
AA
Shimamoto
Tadashi
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004534:Egypt; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D032023:Integrons / Q000235:genetics
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial