Studies of amyloid-like fibrillogenesis through beta-sheet-mediated self-assembly of short synthetic peptides.
Three structurally different types of small peptides, namely, i) Boc-Ile-Aib-Ile-OMe (Aib=alpha-aminoisobutyric acid), ii) Boc-Xx-m-aminobenzoic acid (Xx=beta-Ala and gamma-aminobutyric acid), and iii) Boc-Xx-m-nitroaniline, were found to exhibit beta-sheet-mediated fibrillogenesis in the solid state, revealed by FT-IR, single-crystal X-ray diffraction, and FE-SEM studies. Interestingly, the fibrils grown from peptides 2 and 3 were found to bind with the physiological dye Congo red, a characteristic feature of amyloid fibrils.
Dutt
Anita
A
Department of Chemistry, University of Calcutta, Kolkata-700 009, India.
Spencer
Elinor C
EC
Howard
Judith A K
JA
Pramanik
Animesh
A
D000682:Amyloid / Q000737:chemistry*; D003224:Congo Red / Q000737:chemistry; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008968:Molecular Conformation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary
D000682:Amyloid; D010455:Peptides; D003224:Congo Red