Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera: Curculionidae) midgut.
Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a approximately 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.
Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Building 43421, CEP 91501-970 Porto Alegre, Rio Grande do Sul, Brazil.
Erich Y T
EY
Nakasu
Alexandre A P
AA
Firmino
Simoni C
SC
Dias
Thales L
TL
Rocha
Hudson B
HB
Ramos
Gustavo R
GR
Oliveira
Wagner
W
Lucena
Célia R
CR
Carlini
Maria Fátima
MF
Grossi-de-Sá
D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004064:Digestive System / Q000378:metabolism*; D004731:Endotoxins / Q000737:chemistry / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry / Q000378:metabolism*; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods*; D011485:Protein Binding; D025262:Vacuolar Proton-Translocating ATPases / Q000032:analysis / Q000378:metabolism; D048889:Weevils / Q000378:metabolism*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D050883:HSC70 Heat-Shock Proteins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D025262:Vacuolar Proton-Translocating ATPases