Antinociceptive activities of the methanol extract of the bulbs of Dioscorea bulbifera L. var sativa in mice is dependent of NO-cGMP-ATP-sensitive-K(+) channel activation.
BACKGROUND
Dioscorea bulbifera var sativa is a medicinal plant commonly used in Cameroonian traditional medicine to treat pain and inflammation.


OBJECTIVE
The present work evaluated the effects of the methanol extract of the bulbs of Dioscorea bulbifera in inflammatory and neuropathic models of pain and further investigated its possible mechanism of action.


METHODS
The effects of Dioscorea bulbifera administered orally at the doses of 250 and 500mg/kg were tested in mechanical hypernociception induced by intraplantar (i.pl.) injection of complete Freund's adjuvant (CFA), lipopolysaccharides (LPS) or prostaglandin-E(2) (PGE(2)), as well as in partial ligation sciatic nerve (PLSN), nociception induced by capsaicin and thermal hyperalgesia induced by i.pl. injection of CFA. The therapeutic effects of Dioscorea bulbifera on PGE(2)-induced hyperalgesia were evaluated in the absence and in the presence of l-NAME, an inhibitor of nitric oxide synthase (NOS) and glibenclamide, an inhibitor of ATP-sensitive potassium channels.


RESULTS
The extract showed significant antinociceptive effects in persistent pain induced by CFA and on neuropathic pain induced by PLSN. The effects of Dioscorea bulbifera persisted for 5 days after two administrations in CFA-induced hypernociception. Dioscorea bulbifera significantly inhibited acute LPS-induced pain but failed to reduce thermal hypernociception and capsaicin-induced spontaneous nociception. The antinociceptive effects of this plant extract in PGE(2) model was antagonized by either l-NAME or glibenclamide.


CONCLUSIONS
Present demonstrate the antinociceptive activities of Dioscorea bulbifera both in inflammatory and neuropathic models of pain and these effects may result, at least partially, from its ability to activate the NO-cGMP-ATP-sensitive potassium channels pathway.
Nguelefack
Télesphore B
TB
Laboratory of Animal Physiology and Phytopharmacology, Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Dutra
Rafael C
RC
Paszcuk
Ana F
AF
Andrade
Edinéia L
EL
Tapondjou
Léon A
LA
Calixto
João B
JB
D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism / Q000627:therapeutic use; D005260:Female; D005620:Freund's Adjuvant / Q000009:adverse effects / Q000627:therapeutic use; D005905:Glyburide / Q000009:adverse effects / Q000627:therapeutic use; D006930:Hyperalgesia / Q000139:chemically induced / Q000188:drug therapy*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000009:adverse effects / Q000627:therapeutic use; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000009:adverse effects / Q000627:therapeutic use; D009437:Neuralgia / Q000188:drug therapy*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots / Q000378:metabolism
D054086:KATP Channels; D010936:Plant Extracts; D005620:Freund's Adjuvant; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester; D000432:Methanol