Preliminary enrichment and separation of genistein and apigenin from extracts of pigeon pea roots by macroporous resins.
Enrichment and separation of genistein and apigenin from extracts of pigeon pea roots were studied using eleven macroporous resins with different physical and chemical properties. ADS-5 resin showed the maximum effectiveness among the tested resins. The solute affinity towards ADS-5 resin at different temperatures was described in terms of Langmuir and Freundlich isotherms, and the equilibrium experimental data were well-fitted to the two isotherms. In order to optimize the operating parameters for separating genistein and apigenin, dynamic adsorption and desorption tests were carried out. After one run treatment with ADS-5 resin, the contents of genistein and apigenin in the product were 9.36-fold and 11.09-fold increased with recovery yields of 89.78% and 93.41%, respectively. The process achieved easy and effective enrichment and separation of genistein and apigenin by using ADS-5 resin, and it is a promising basis for large-scale preparation of genistein and apigenin from pigeon pea or other plants extracts.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Wei
W
Liu
Su
S
Zhang
Yuan-Gang
YG
Zu
Yu-Jie
YJ
Fu
Wei
W
Ma
Dong-Yang
DY
Zhang
Yu
Y
Kong
Xiao-Juan
XJ
Li
D000327:Adsorption; D047310:Apigenin / Q000302:isolation & purification*; D019833:Genistein / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D018532:Pisum sativum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D013696:Temperature
D010936:Plant Extracts; D012117:Resins, Synthetic; D047310:Apigenin; D019833:Genistein