[Establishment of a cell culture model of corpus cavernosum smooth muscle cells derived from diabetic rats with erectile dysfunction].
OBJECTIVE
To investigate the method for culturing corpus cavernosum smooth muscle cells (CCSMs) derived from diabetic rats with erectile dysfunction (ED) for the study of ED caused by diabetes.


METHODS
CCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture. The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed.


RESULTS
The primary culture of CCSM was performed successfully, and the cells were seen to migrate from the small tissue pieces 3 days later, reaching nearly confluence in 16-18 days. A typical "hill-valley" growth pattern was noted in the cell passaging. Immunohistochemical staining for alpha-smooth muscle actin (alpha-SM-actin) and desmin yielded positive results in the cells.


CONCLUSIONS
The modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED, and the cultured CCSMs display a faster proliferation than normal CCSMs. No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.
Ye
Ting-yu
TY
Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. tingyuye@yahoo.com.cn
Wei
An-yang
AY
Wan
Bo
B
Yang
Yong
Y
Luo
Xin-gui
XG
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D007172:Erectile Dysfunction / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D010410:Penile Erection; D010413:Penis / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley