Differential influence of non-synaptic mechanisms in two in vitro models of epileptic field bursts.
Non-synaptic interactions are known to promote epileptiform activity through mechanisms that have primarily been studied in one particular in vitro model (low Ca(2+) model). Here we characterize another non-synaptic model, where ictal-like field bursts are induced in the CA1 area of rat hippocampal slices by exposure to Cs(+) (4-5mM) together with blockers of fast chemical synaptic transmission, and compare it with the low Ca(2+) model. The Cs-induced field bursts were blocked by 1 microM tetrodotoxin, but persisted in the presence of 200 microM Cd(2+) or 300 microM Ni(2+). Hyperosmotic condition (addition of 30 mM sucrose), reduced burst amplitude, but, unlike field bursts induced by 0mM Ca(2+)/5.25 mM K(+), sucrose had no effect on frequency or duration. Intracellular alkalinization-acidification sequence induced by NH(4)Cl potentiated and blocked, respectively, the field bursts. Octanol (100-250 microM) blocked all activity in most experiments. A quantitative comparison of three gap junction antagonists (carbenoxolone (100 microM), quinidine (100-250 microM), and endothelin-3 (1-2 microM)) indicated that gap junction communication is implicated in both models. However, endothelin-3 had selective effect on the low Ca(2+)-induced field burst. The data suggest that extracellular space-dependent processes, including field effects, significantly contribute to ongoing field burst activity, whereas initiation of a field burst can occur with or without the aid of such interactions, depending on the level of neuronal excitability. Gap junctions seem to have a general role in initiating field bursts. However, the contribution to this effect from neuronal versus glial connexin types differs in the two epileptic models studied.
Department of Physiology and Biophysics, Aarhus University, Arhus C, Denmark.
Nikolaj
N
Kraglund
Mogens
M
Andreasen
Steen
S
Nedergaard
D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000503:physiopathology*; D004827:Epilepsy / Q000503:physiopathology*; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013997:Time Factors