A nonviral minicircle vector for deriving human iPS cells.
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.
Fangjun
F
Jia
Kitchener D
KD
Wilson
Ning
N
Sun
Deepak M
DM
Gupta
Mei
M
Huang
Zongjin
Z
Li
Nicholas J
NJ
Panetta
Zhi Ying
ZY
Chen
Robert C
RC
Robbins
Mark A
MA
Kay
Michael T
MT
Longaker
Joseph C
JC
Wu
D000328:Adult; D004270:DNA, Circular / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D014162:Transfection
D004270:DNA, Circular