A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device.
Porcine articular cartilage tissue was crushed using Multi-Beads-Shocker() and digested with trypsin and collagenase type II. Chondrocyte cell yield was maximized by automatic crushing for 10 s, which was 4 times that by manual dicing with a surgical blade and might be useful for the automation of the cell processing.
Hokkaido University, Sapporo, Japan. takagi-m@eng.hokudai.ac.jp
Mutsumi
M
Takagi
Hiromichi
H
Yoshioka
Shigeyuki
S
Wakitani
D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D017364:Collagenases / Q000378:metabolism; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D014357:Trypsin / Q000378:metabolism
D014357:Trypsin; D017364:Collagenases