Effect of di(2-ethylhexyl) phthalate (DEHP) on lipolysis and lipoprotein lipase activities in adipose tissue of rats.
The di(2-ethylhexyl) phthalate (DEHP) is an ubiquitous environmental chemical with detrimental health effects. The present work was designed to asses some potential mechanisms by which DEHP causes, among others, a reduced body fat retention. Since this effect could be related to an alteration of adipocyte triacylglycerol (TG) metabolism, we evaluated the effects of dietary DEHP in adipose tissues upon (1) the number and size of fat cells; (2) the basal and stimulated lipolysis and (3) the lipoprotein lipase (LPL) activity. Groups of male Wistar rats were fed for 21 days a control diet alone (control group) or the same control diet supplemented with 2% (w/w) of DEHP (DEHP group). The LPL activity of DEHP-fed rats was increased in lumbar and epididymal adipose tissues. These rats had significantly reduced weight in epididymal and lumbar tissues, together with reduced size of epididymal adipocytes. These alterations do not seem to be associated with higher lipid mobility because neither basal lipolysis nor 'in vitro' stimulated lipolysis by noradrenaline (NA) showed to be modified by DEHP. Based on these results, we concluded that the adipose tissue size reduction induced by DEHP intake is not due to changes in lipolysis nor to a decreased LPL activity. More research is needed to achieve a comprehensive understanding of the potential mechanisms by which DEHP causes, among others, a reduced body fat retention.
Departamento de Ciencias Biológicas, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Marcela I
MI
Martinelli
Norberto O
NO
Mocchiutti
Claudio A
CA
Bernal
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D052436:Adipose Tissue, White / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D050154:Adiposity; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D002149:Energy Intake / Q000187:drug effects; D004785:Environmental Pollutants / Q000494:pharmacology*; D005506:Food Contamination; D050152:Intra-Abdominal Fat / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010968:Plasticizers / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D004785:Environmental Pollutants; D010968:Plasticizers; D014280:Triglycerides; D004051:Diethylhexyl Phthalate; D008071:Lipoprotein Lipase; D009638:Norepinephrine