The effect of AlloDerm on the initiation and growth of human neovessels.
OBJECTIVE
AlloDerm (LifeCell Corp., Branchburg, NJ) is commonly employed for reconstruction of ablative soft tissue and mucosal defects following surgical resections. Although devoid of growth factors, AlloDerm may serve as an adhesive matrix for binding of growth factors, increasing local angiogenesis, and wound healing. We hypothesized that AlloDerm would enhance angiogenesis and might be altered with autologous blood products to enhance initiation of the angiogenic response.


METHODS
We used a human placental vein in a fibrin-thrombin clot-based angiogenesis model. Four groups, human placental vein (HPVM), HPVM with AlloDerm, HPVM with AlloDerm plus platelet-poor plasma, and HPVM with AlloDerm plus platelet-rich plasma were evaluated. Endothelial cell growth was evaluated visually (40x). Hematoxylin and eosin staining and immunofluorescent staining for growth within the AlloDerm matrix were also performed. To assess human umbilical vein endothelial cell (HUVEC) sites of attachment to AlloDerm, we incubated HUVEC cells with AlloDerm for a period of 2 weeks and evaluated attachment with anti-factor VIII immunofluorescence.


RESULTS
Angiogenic initiation decreased in the combined placental vein with AlloDerm group (P < .0001 at day 7, 14, 21). Additionally, initiation in the AlloDerm plus platelet-poor plasma group was significantly better than the AlloDerm alone group when placentas 2 and 3 were compared (P < .0001). On hematoxylin and eosin staining and immunofluorescent factor VIII staining, no endothelial growth into the AlloDerm was noted in the samples analyzed.


CONCLUSIONS
AlloDerm may be enriched with platelet-poor plasma to stimulate greater initiation and wound healing; however, AlloDerm inhibits angiogenic initiation in this model.
Department of Otorhinolaryngology-Head and Neck Surgery, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA.
Sean R
SR
Weiss
Justin M
JM
Tenney
Jessica L
JL
Thomson
Catherine T
CT
Anthony
Ernest S
ES
Chiu
Paul L
PL
Friedlander
Eugene A
EA
Woltering
D016009:Chi-Square Distribution; D003094:Collagen / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects; D005260:Female; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010920:Placenta / Q000098:blood supply*; D053657:Platelet-Rich Plasma; D011247:Pregnancy; D013194:Staining and Labeling; D014471:Umbilical Veins
C440568:Alloderm; D003094:Collagen