Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae.
Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4gL(-1) ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.
School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea. gcdu@jiangnan.edu.cn
Kyung Ok
KO
Yu
Seung Wook
SW
Kim
Sung Ok
SO
Han
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism
D000431:Ethanol; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D005990:Glycerol