Inorganic polyphosphate differentiates human mesenchymal stem cells into osteoblastic cells.
The existence of inorganic polyphosphates [poly(P)] in human cells has been demonstrated. In osteoblasts, it is suggested that the concentration of cellular poly(P) is relatively high. In this study, we examined whether poly(P) accelerates the differentiation of human mesenchymal stem cells (hMSCs) from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) into osteoblastic cells. Alkaline phosphatase (ALP) activity was induced by poly(P) in hMSCs from both OA and RA. In Alizarin Red S and osteocalcin EIA, there was a significant difference between the control and poly(P) group. In real-time PCR, there was a significant difference in ALP, collagen type 1A, osteocalcin, and bone sialoprotein between the control and poly(P) group. Our findings suggest that poly(P) have the potent role of differentiating hMSCs into osteoblastic cells at the early and later stages of osteoblastic differentiation.
Department of Orthopedics, Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan.
Daiki
D
Morimoto
Tetsuya
T
Tomita
Shoko
S
Kuroda
Chikahisa
C
Higuchi
Shinichi
S
Kato
Toshikazu
T
Shiba
Hironori
H
Nakagami
Ryuichi
R
Morishita
Hideki
H
Yoshikawa
D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000494:pharmacology*
D011122:Polyphosphates; D015675:Osteocalcin; D000469:Alkaline Phosphatase