Isoprene emission protects photosynthesis in sunfleck exposed Grey poplar.
In the present study, we combined transient temperature and light stress (sunfleck) and comparably analyzed photosynthetic gas exchange in Grey poplar which has been genetically modified in isoprene emission capacity. Overall, we demonstrate that for poplar leaves the ability to emit isoprene is crucial to maintain photosynthesis when exposed to sunflecks. Net CO2 assimilation and electron transport rates were strongly impaired in sunfleck-treated non-isoprene emitting poplars. Similar impairment was not detected when the leaves were exposed to high light (lightflecks) only. Within 10 h non-isoprene emitting poplars recovered from sunfleck-related impairment as indicated by chlorophyll fluorescence and microarray analysis. Unstressed leaves of non-isoprene emitting poplars had higher ascorbate contents, but also higher contents of malondialdehyde than wild-type. Microarray analyses revealed lipid and chlorophyll degradation processes in the non-isoprene emitting poplars. Thus, there is evidence for an adjustment of the antioxidative system in the non-isoprene emitting poplars even under normal growth conditions.
Behnke
Katja
K
Karlsruhe Institute of Technology, Institute for Meteorology and Climate Research (IMK-IFU), Garmisch-Partenkirchen, Germany.
Loivamäki
Maaria
M
Zimmer
Ina
I
Rennenberg
Heinz
H
Schnitzler
Jörg-Peter
JP
Louis
Sandrine
S
D002070:Butadienes; D002245:Carbon Dioxide / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D045782:Hemiterpenes / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010420:Pentanes; D010788:Photosynthesis / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology; D018526:Plant Transpiration / Q000502:physiology; D032107:Populus / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology; D013472:Sunlight; D013696:Temperature
D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D002245:Carbon Dioxide