Entamoeba histolytica: expression and localization of Gal/GalNAc lectin in virulent and non-virulent variants from HM1:IMSS strain.
We have purified Gal/GalNAc lectin from Entamoeba histolytica by electroelution. The purified protein was used to immunize rabbits and obtain polyclonal IgG's anti-lectin. These antibodies were used as tools to analyze the expression and localization of the amoebic lectin in both virulent (vEh) and non-virulent (nvEh) variants of axenically cultured HM1:IMSS strain. vEh is able to induce liver abscesses in hamsters, whereas nvEh has lost this ability. In vitro, amoebic trophozoites from both variants equally express this protein as shown by densitometric analysis of the corresponding band in Western blots from lysates. In both types of trophozoites, the pattern of distribution of the lectin was mainly on the surface. We have also compared by immunohistochemistry the presence and distribution of lectin in the in vivo liver lesions produced in hamsters. In order to prolong the survival of nvEh to analyze both variants in an in vivo model, hamsters inoculated with nvEh were treated with methyl prednisolone. Our results suggest that the Gal/GalNAc lectin is equally expressed in both nvEh and vEh.
López-Vancell
R
R
Medicine Faculty, National Autonomus University of Mexico, General Hospital of Mexico, Mexico City, Mexico. lvsario@yahoo.com.mx
Arreguín Espinosa
R
R
González-Canto
A
A
Néquiz Avendaño
M
M
García de León
M C
MC
Olivos-García
A
A
López-Vancell
D
D
Pérez-Tamayo
R
R
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D006224:Cricetinae; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity*; D007150:Immunohistochemistry; D037102:Lectins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008101:Liver Abscess, Amebic / Q000276:immunology / Q000469:parasitology; D008297:Male; D011817:Rabbits; D053058:Trophozoites / Q000276:immunology / Q000378:metabolism; D014774:Virulence
D000913:Antibodies, Protozoan; D015295:Antigens, Tumor-Associated, Carbohydrate; D037102:Lectins; C016467:Thomsen-Friedenreich antigen