Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diagnosing hydatidosis.
Echinococcus granulosus causes human cystic echinococcosis as an important public health problem in many regions of the world. There are some problems in primary diagnosis such as cross-reaction with sera from patients with other parasitic disease in serological tests. The use of an appropriate source of antigenic material is a very important and crucial point in the improvement of the serodiagnostic features such as enzyme-linked immunosorbent assay (ELISA) method. We expressed and purified recombinant AgB of Echinococcus granulosus and used as antigen in ELISA method. Serum samples were given from 36 cystic hydatid disease patients that have been confirmed by surgical operation as well as 36 healthy individuals sera were tested by ELISA method using recombinant AgB and compared with commercial kit (Euroimmun) for specificity and sensitivities value. The sensitivity of 91.66% and specificity of 97.22% were determined by homemade kit.
Department of Parasitology And Mycology, Shahid Beheshti University, MC, Tehran, Iran.
Elham
E
Kalantari
Mojgan
M
Bandehpour
Ramin
R
Pazoki
Niloofar
N
Taghipoor-Lailabadi
Hooshang
H
Khazan
Nariman
N
Mosaffa
Mohammad Reza
MR
Nazaripouya
Bahram
B
Kazemi
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004443:Echinococcosis / Q000175:diagnosis*; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008074:Lipoproteins; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D008074:Lipoproteins; D011994:Recombinant Proteins; C514022:antigen B, Echinococcus granulosus