A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance.
A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 0.1-150 nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5.
International Centre of Biodynamics, Intrarea Portocalelor 1B, 060101 Bucharest, Romania.
Cristina
C
Polonschii
Sorin
S
David
Sara
S
Tombelli
Marco
M
Mascini
Mihaela
M
Gheorghiu
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D006046:Gold / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D012710:Serum Albumin, Bovine; D006046:Gold; D013917:Thrombin