Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum.
The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15(500) (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density.
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo 060-8628, Japan.
Masashi
M
Fujiwara
Yu
Y
Aizu
Itaru
I
Shioya
Mutsumi
M
Takagi
D000818:Animals; D016466:CHO Cells; D002417:Cattle / Q000097:blood*; D018929:Cell Culture Techniques / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005399:Fishes / Q000097:blood*; D044967:Serum / Q000378:metabolism*
D016895:Culture Media, Serum-Free