Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima.
Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.
Huang
Z
Z
Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan, China.
Long
H
H
Jiang
Q-T
QT
Wei
Y-M
YM
Yan
Z-H
ZH
Zheng
Y-L
YL
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics*; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D011003:Ploidies; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics
D018744:DNA, Plant; D005983:Glutens; C037302:glutenin