Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients.
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Virology Laboratory, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Maria do Carmo
Mdo C
Debur
Luine R Rosele
LR
Vidal
Meri Bordignon
MB
Nogueira
Luciane Aparecida
LA
Pereira
Gislene R A
GR
Takahashi
Indianara
I
Rotta
Sérgio Monteiro de
SM
Almeida
Sonia Mara
SM
Raboni
D000328:Adult; D003587:Cytomegalovirus / Q000276:immunology* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D016867:Immunocompromised Host / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D010750:Phosphoproteins / Q000032:analysis*; D011247:Pregnancy; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000032:analysis*; D014779:Virus Replication
D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa