[Effect of FK506 on cytokine secretion in whole blood].
OBJECTIVE
To investigate the effect of FK506 on cytokine secretions in whole blood from healthy individuals.


METHODS
Blood samples collected from healthy volunteers were co-cultured with different concentrations of FK506 and stimulated with PMA and IONO. The concentrations of 8 cytokines including IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF were detected by Bio-Plex suspension system.


RESULTS
Compared with the control group, high-concentration FK506 (20 ng/ml) significantly inhibited the secretions of IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF. At a moderate concentration (5 ng/ml), FK506 inhibited the secretion of GM-CSF significantly.


CONCLUSIONS
FK506 effectively inhibits the secretion of proinflammatory cytokines including IL-6, IFN-gamma and TNF-alpha and also the secretion of IL-2, IL-12, IL-17, GM-CSF and G-CSF. FK506 might play the role of immunosuppression by inhibiting the production of these cytokines by the immune cells. Monitoring the levels of these cytokines might be a potential method for evaluating the adequacy of FK506 doses administered.
Yi
He
H
Institute of Transplant Immunology, Department of Organ Transplant, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. heyidiyi@163.com
Min
Li
L
Liu
Zhan-guo
ZG
Luo
Yu-wei
YW
Wang
Ming
M
Zhang
Jie
J
Sun
Er-wei
EW
D000328:Adult; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008297:Male; D016559:Tacrolimus / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism; D055815:Young Adult
D016207:Cytokines; D007166:Immunosuppressive Agents; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016559:Tacrolimus