An efficient treatment for detoxification process of cassava starch by plant cell wall-degrading enzymes.
The objective of this work was to remove linamarin in starch from cassava (Manihot esculenta Crantz cv. KU-50) roots, a high-cyanogen variety by using plant cell wall-degrading enzymes, xylanase and cellulase. The combination of xylanase from Bacillus firmus K-1 and xylanase and cellulase from Paenibacillus curdlanolyticus B-6 at the ratio of 1:9 showed the maximum synergism at 1.8 times for hydrolyzing cassava cortex cell walls and releasing linamarase. Combined enzyme treatment enhanced linamarin liberation from the parenchyma by 90%. In addition, when the combined enzymes were applied for detoxification during cassava starch production, a low-cyanide-product was obtained with decreased linamarin concentration (96%) compared to non-enzyme treated tissues. Based on these results, xylanase and cellulase treatment is a good method for low-cyanide-cassava starch production and could be applied for detoxification of cassava products during processing.
King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok, Thailand.
Somphit
S
Sornyotha
Khin Lay
KL
Kyu
Khanok
K
Ratanakhanokchai
D001407:Bacillus / Q000201:enzymology; D002473:Cell Wall / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D002365:Manihot / Q000737:chemistry* / Q000378:metabolism; D009570:Nitriles / Q000032:analysis; D056507:Paenibacillus / Q000201:enzymology; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D013213:Starch / Q000378:metabolism*; D013696:Temperature
D009570:Nitriles; D013213:Starch; C005091:linamarin