Excited states of fluorescent proteins, mKO and DsRed: chromophore-protein electrostatic interaction behind the color variations.
The emitting states of green fluorescent protein (GFP), monomeric Kusabira orange (mKO), and Discosoma red (DsRed) were studied using QM/MM and SAC-CI methods. By comparing the electronic structures among the green-, orange-, and red-emitting states as well as their electrostatic and quantum mechanical interactions within the protein cavity, the basic mechanisms for determining emission colors have been clarified. We found that the orange and red emissions of mKO and DsRed, respectively, result from cancellation between two effects, the pi skeleton extension (red shift) and protein electrostatic potential (blue shift). The extension of the pi skeleton enhances the intramolecular charge-transfer character of the transition, which makes the fluorescence energy more sensitive to the protein's electrostatic potential. On the basis of this mechanism, we predicted amino acid mutations that could red shift the emission energy of DsRed. A novel single amino acid mutation, which was examined computationally, reduced the DsRed emission energy from 2.14 (579 nm) to 1.95 eV (636 nm), which is approaching near-infrared fluorescence.
Hasegawa
Jun-ya
JY
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nshikyo-ku, Kyoto 615-8510, Japan. hasegawa@sbchem.kyoto-u.ac.jp
Ise
Takehiko
T
Fujimoto
Kazuhiro J
KJ
Kikuchi
Akihiro
A
Fukumura
Eiko
E
Miyawaki
Atsushi
A
Shiro
Yoshitsugu
Y
D003198:Computer Simulation; D008164:Luminescent Proteins / Q000737:chemistry*; D008956:Models, Chemical; D011789:Quantum Theory; D055672:Static Electricity; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C413663:fluorescent protein 583