Aberrant expression of NK cell receptors in Epstein-Barr virus-positive gammadelta T-cell lymphoproliferative disorders.
To clarify the difference between gene expression signature of Epstein-Barr virus (EBV)-positive gammadelta T-cell lymphoproliferative disorders (LPD) and those in normal gammadelta T-cells, we set out to determine gene expression profiling using three gammadelta T-cell lines derived from patients with EBV-positive gammadelta T-cell LPD. Gene expression profiling using an Affymetrix Genechip revealed a unique gene expression pattern of NK cell receptors in gammadelta T-LPD cell lines. Genes encoding killer cell lectin-like receptors (KLR), such as KLRG1 and KLRB1 (CD161), were generally low in gammadelta T-LPD cell lines, while killer cell immunoglobulin-like receptors varied among cell lines. In addition, expression of CD161 surface antigen was found to be remarkably decreased in gammadelta T-LPD cell lines by flow cytometric analysis. We conclude that gammadelta T-cell expansion in EBV-positive gammadelta T-LPD is in part due to inappropriate expression of the NK cell receptors which related to impaired function cytotoxic T cells.
First Department of Internal Medicine, Tokyo Medical University, Japan.
Yu
Y
Zhang
Junko H
JH
Ohyashiki
Norio
N
Shimizu
Kazuma
K
Ohyashiki
D015703:Antigens, CD / Q000032:analysis; D045744:Cell Line, Tumor / Q000276:immunology / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D016130:Immunophenotyping; D039661:Integrin beta3 / Q000096:biosynthesis* / Q000235:genetics; D037181:Lectins, C-Type / Q000096:biosynthesis* / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000821:virology; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
D015703:Antigens, CD; C061248:ITGB3 protein, human; D039661:Integrin beta3; C527203:KLRB1 protein, human; C113413:KLRG1 protein, human; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; D015534:Trans-Activators