Egg incubation and collection of painted turtle embryos.
This protocol describes how to incubate painted turtle eggs and collect embryos for gene expression analysis. The same basic protocol can be used to incubate eggs and collect embryos from other turtle species by modifying the incubation temperature to suit the particular developmental needs of each species.
Valenzuela
Nicole
N
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA. nvalenzu@iastate.edu
D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology; D020869:Gene Expression Profiling / Q000379:methods; D005821:Genetic Techniques; D007756:Laboratory Animal Science / Q000379:methods*; D010063:Ovum / Q000166:cytology*; D013696:Temperature; D014426:Turtles / Q000196:embryology* / Q000235:genetics*