[Typeability of AmpFlSTR SGM Plus loci in kidney specimens incubated in different environments].
In consecutive stages of postmortem decomposition human hard tissues e.g. bones and teeth have been the most suitable material for genetic identification, however, its processing and DNA extraction is relatively costly and time-consuming. The aim of the study was the assessment of the environmental effect on typeability of AmpFlSTR SGM Plus loci in the kidney. The specimens were collected during autopsies of five persons aged 20-30 years with post mortem interval (PMI) limited to 14 hours, and subsequently incubated at 21 degrees C and 4 degrees C in different environmental conditions. DNA was extracted by the organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. The fastest decline of SGM Plus profiles was noted in specimens incubated at 21 degrees C in closed containers. The specimens incubated at 4 degrees C in closed containers were the most stable for SGM Plus typing. The differences in typeability rates for specimens incubated at 21degrees C in open vs. closed containers can be attributed to desiccation of the outermost layers of the former specimens.
Zakład Medycyny Sadowej Akademii Medycznej w Białymstoku, ul. Kilińskiego 1, 15-230 Białystok.
Anna
A
Niemcunowicz-Janica
Witold
W
Pepiński
Jerzy
J
Janica
Iwona
I
Ptaszyńska-Sarosiek
Małgorzata
M
Skawrońska
Ewa
E
Koc-Zórawska
D000328:Adult; D001344:Autopsy / Q000379:methods*; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D011180:Postmortem Changes; D013048:Specimen Handling; D020080:Tandem Repeat Sequences; D014022:Tissue Survival