Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis.
Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated by various neuropeptides. We cloned the gene and analyzed the expression of the prothoracicostatic peptide, a unique neuropeptide shown to suppress the production of ecdysteroids in the prothoracic gland of the silkworm, Bombyx mori. We also characterized a Bombyx G protein-coupled receptor, which has previously been identified as an ortholog of the Drosophila sex peptide receptor, as a functional prothoracicostatic peptide receptor. This receptor responded specifically to the prothoracicostatic peptides when examined using a heterologous expression system. The receptor was highly expressed in the prothoracic gland on the day before each larval and pupal ecdysis, when prothoracicostatic peptides are synthesized at a high level in the epiproctodeal glands. These results suggest that the sex peptide receptor functions as a prothoracicostatic peptide receptor in Bombyx and that the peripheral neurosecretory cells as well as the central neuroendocrine system play stage-specific roles in regulating ecdysteroidogenesis.
Yamanaka
Naoki
N
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan.
Hua
Yue-Jin
YJ
Roller
Ladislav
L
Spalovská-Valachová
Ivana
I
Mizoguchi
Akira
A
Kataoka
Hiroshi
H
Tanaka
Yoshiaki
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D026461:Ecdysteroids / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D012739:Gonadal Steroid Hormones / Q000235:genetics / Q000378:metabolism*; D007301:Insect Hormones / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D017931:DNA Primers; D026461:Ecdysteroids; D012739:Gonadal Steroid Hormones; D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C400522:alanyl-tryptophyl-glutaminyl-aspartyl-leucyl-asparagyl-seryl-alanyl-tryptophanamide