[Analysis of prognostic factors of retinoblastoma].
OBJECTIVE
To study the prognostic factors of retinoblastoma (RB).


METHODS
Prospective case series study.With Envision System, surgical specimens from 31 RB cases were immunostained by using anti-NSE, GFAP and S-100 antibodies. Number of NSE positive cells was counted at 1000 tumor cells in each slice. The morphology of GFAP and S-100 stained positive cells were analyzed. All cases were followed-up for 5 to 17 years. Cox regression pattern was adopted to analyze the relationship between sex, age, stage, differentiation, number of NSE positive cells, the sizes of GFAP and S-100 positive cells, therapies and prognosis.


RESULTS
(1) Almost all cases were stained positively by anti-NSE antibodies. Positive rates of GFAP and S-100 expression specimens were 61.29% (19/31) and 58.06% (18/31), respectively. (2) The number of NSE positive cells was correlated with a better prognosis (P = 0.0361). The sizes of GFAP and S-100 stained area were also related with a better progress (P = 0.0430, 0.0477). In patients with a greater number of NSE stained cells (averaged 324/1000), the survival time was >/= 5 years. In patients with a less number of NSE stained cells (averaged 182/1000), the survival time was less than 5 years. (3) There was a significant correlation between the number of NSE stained cells and the results for GFAP and S-100 staining (r = 0.547, 0.581), respectively.


CONCLUSIONS
The number of NSE positive cells is correlated with a better prognosis of RB. The expression of GFAP and S-100 protein are also correlated with a better prognosis of RB. The number of NSE positive cells and the expression of GFAP and S-100 protein may be regarded as the standards in judging the degree of differentiation of RB, and may be used as one of criteria for the estimation of the prognosis.
Department of Ophthalmology, Beijing Electric Power Hospital, Beijing 100073, China.
Ying
Y
Lu
Jin-fen
JF
Wang
D002675:Child, Preschool; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006801:Humans; D007223:Infant; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D011379:Prognosis; D011446:Prospective Studies; D019572:Retinal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D012175:Retinoblastoma / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology
D005904:Glial Fibrillary Acidic Protein; D010751:Phosphopyruvate Hydratase