Human ovarian tissue cryopreservation: quality of follicles as a criteria of effectiveness.
Experiments comparing vitrification and conventional freezing of mammalian ovarian tissue show that vitrification can also guarantee the storage of viable follicles after warming, but conventional freezing is more effective. The central goal of cryotechnology is the preservation of intact follicles. This article presents a critical opinion about the normality of follicles after vitrification of human ovarian tissue and microbial contamination as a result of direct contact of this tissue with liquid nitrogen at vitrification.
Department of Obstetrics and Gynaecology, University of Ulm, Prittwitzstrasse 43, Ulm, Germany.
V
V
Isachenko
E
E
Isachenko
R
R
Kreienberg
M
M
Woriedh
Jm
J
Weiss
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D005615:Freezing; D006801:Humans; D009584:Nitrogen / Q000009:adverse effects; D006080:Ovarian Follicle; D058989:Vitrification
D003451:Cryoprotective Agents; D009584:Nitrogen