Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste.
Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste.
Synchrotron Light Research Institute (Public Organization), 111 University Avenue, Nakhon Ratchasima 30000, Thailand.
Chomphunuch
C
Songsiriritthigul
Sasithorn
S
Lapboonrueng
Phornsiri
P
Pechsrichuang
Puntarika
P
Pesatcha
Montarop
M
Yamabhai
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D017931:DNA Primers; D011994:Recombinant Proteins; D002688:Chitinases