Association of oxidative stress and GST-T1 gene with childhood bronchial asthma.
There is increasing evidence that bronchial asthma is associated with increased oxidative stress. Reactive oxygen species are produced in each organism as by-products of several essential reactions and can damage biomolecules. Asthma is a complex chronic inflammatory disorder of the airways, with many candidate genes suspected as being important in its development. Glutathione S-transferase (GST) is such a gene due to its role in protection against oxidative stress. In the present study, we examined the hypothesis that increased oxidative stress and polymorphism in the GST-T1 gene are associated with childhood asthma. We found that the amount of sulfhydryl groups significantly decreased and the content of thiobarbituric acid-reactive substances increased in the group of asthmatic children, compared with healthy controls. The GST-T1 null genotype was more frequent among the asthma patients. These results suggest that the GST-T1 null genotype and increased oxidative stress may play a role in the asthma pathogenesis in children.
Department of Medical Biochemistry, Comenius University in Bratislava, Jessenius Faculty of Medicine, Martin, Slovakia. eva.babusikova@gmail.com
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Babusikova
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Jesenak
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Kirschnerova
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P
Banovcin
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Dobrota
D000293:Adolescent; D000367:Age Factors; D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D002648:Child; D005260:Female; D020868:Gene Silencing; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase