Hydrogel/calcium phosphate composites require specific properties for three-dimensional culture of human bone mesenchymal cells.
To provide multipotent cells with a three-dimensional environment closer to bone matrix, an engineered construct mimicking bone components has been designed and evaluated. A biocompatible hydrogel (silated hydroxypropylmethyl cellulose) was used as an extra-cellular matrix while biphasic calcium phosphate ceramic particles were used to replace mineralized matrix. Finally, human bone mesenchymal cells were cultured in three dimensions in the resulting constructs to study their cell viability, proliferation, interactions within the composites, and maintenance of their osteogenic potential. This approach resulted in homogeneous structures in which cells were viable and retained their osteoblastic differentiation potential. However, the cells did not proliferate nor colonize the constructs, possibly because of a lack of suitable interactions with their micro-environment.
Laboratory for Osteo-Articular and Dental Tissue Engineering, INSERM U791, University of Nantes, 1 Pl. A. Ricordeau, 44042 Nantes, France. jerome.sohier@univ-nantes.fr
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Sohier
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Corre
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Weiss
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Layrolle
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology; D019149:Bioreactors; D001842:Bone and Bones / Q000166:cytology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D013194:Staining and Labeling; D046509:Tissue Culture Techniques / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000469:Alkaline Phosphatase